LOCUS       DAVBGY010000001        85095 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVBGY010000001 DAVBGY010000000
VERSION     DAVBGY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 85095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85095
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.001"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(144..731)
                     /locus_tag="VQ620_000001"
     CDS             complement(144..731)
                     /locus_tag="VQ620_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2762711.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            complement(824..967)
                     /locus_tag="VQ620_000002"
     CDS             complement(824..967)
                     /locus_tag="VQ620_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2762712.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            complement(980..1360)
                     /locus_tag="VQ620_000003"
     CDS             complement(980..1360)
                     /locus_tag="VQ620_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET2762713.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            complement(1353..1693)
                     /locus_tag="VQ620_000004"
                     /pseudo
     CDS             complement(1353..1693)
                     /locus_tag="VQ620_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1707..1955)
                     /locus_tag="VQ620_000005"
                     /pseudo
     CDS             complement(1707..1955)
                     /locus_tag="VQ620_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            complement(1955..2125)
                     /locus_tag="VQ620_000006"
     CDS             complement(1955..2125)
                     /locus_tag="VQ620_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2762714.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            complement(2312..2680)
                     /gene="rplL"
                     /locus_tag="VQ620_000007"
     CDS             complement(2312..2680)
                     /gene="rplL"
                     /locus_tag="VQ620_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET2762715.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKTKLEEAGASVTLK"
     gene            complement(2756..3256)
                     /locus_tag="VQ620_000008"
     CDS             complement(2756..3256)
                     /locus_tag="VQ620_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET2762716.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     misc_feature    complement(3295..3430)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3506..4765)
                     /locus_tag="VQ620_000009"
     CDS             complement(3506..4765)
                     /locus_tag="VQ620_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HET2762717.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQIQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     gene            complement(5067..6815)
                     /locus_tag="VQ620_000010"
     CDS             complement(5067..6815)
                     /locus_tag="VQ620_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2762718.1"
                     /translation="MKRQTVNQTLKRLVVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKELSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTISRVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            complement(6817..8541)
                     /locus_tag="VQ620_000011"
     CDS             complement(6817..8541)
                     /locus_tag="VQ620_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2762719.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFLSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENLPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            complement(8599..9537)
                     /gene="msrB"
                     /locus_tag="VQ620_000012"
     CDS             complement(8599..9537)
                     /gene="msrB"
                     /locus_tag="VQ620_000012"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET2762720.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            complement(9679..9756)
                     /locus_tag="VQ620_000013"
                     /pseudo
     CDS             complement(9679..9756)
                     /locus_tag="VQ620_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(9832..10701)
                     /locus_tag="VQ620_000014"
     CDS             complement(9832..10701)
                     /locus_tag="VQ620_000014"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET2762721.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTKGVRVEAK"
     gene            complement(10703..11989)
                     /locus_tag="VQ620_000015"
     CDS             complement(10703..11989)
                     /locus_tag="VQ620_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET2762722.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            complement(12140..12877)
                     /gene="mecA"
                     /locus_tag="VQ620_000016"
     CDS             complement(12140..12877)
                     /gene="mecA"
                     /locus_tag="VQ620_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HET2762723.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSFEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            complement(13077..14111)
                     /locus_tag="VQ620_000017"
     CDS             complement(13077..14111)
                     /locus_tag="VQ620_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET2762724.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            complement(14140..15573)
                     /locus_tag="VQ620_000018"
     CDS             complement(14140..15573)
                     /locus_tag="VQ620_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HET2762725.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFIAKMLTKEN"
     gene            complement(15589..16575)
                     /locus_tag="VQ620_000019"
     CDS             complement(15589..16575)
                     /locus_tag="VQ620_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2762726.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQIYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            complement(16577..17680)
                     /locus_tag="VQ620_000020"
     CDS             complement(16577..17680)
                     /locus_tag="VQ620_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET2762727.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDLEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQIIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            complement(17670..18515)
                     /locus_tag="VQ620_000021"
     CDS             complement(17670..18515)
                     /locus_tag="VQ620_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2762728.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLTLDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            complement(18794..20068)
                     /gene="psr"
                     /locus_tag="VQ620_000022"
     CDS             complement(18794..20068)
                     /gene="psr"
                     /locus_tag="VQ620_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HET2762729.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            complement(20065..20913)
                     /gene="pheA"
                     /locus_tag="VQ620_000023"
     CDS             complement(20065..20913)
                     /gene="pheA"
                     /locus_tag="VQ620_000023"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET2762730.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYIDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            complement(20910..21386)
                     /locus_tag="VQ620_000024"
     CDS             complement(20910..21386)
                     /locus_tag="VQ620_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET2762731.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRHVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            complement(21379..22662)
                     /gene="aroA"
                     /locus_tag="VQ620_000025"
     CDS             complement(21379..22662)
                     /gene="aroA"
                     /locus_tag="VQ620_000025"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET2762732.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            complement(22754..23092)
                     /locus_tag="VQ620_000026"
     CDS             complement(22754..23092)
                     /locus_tag="VQ620_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HET2762733.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            complement(23103..24206)
                     /locus_tag="VQ620_000027"
     CDS             complement(23103..24206)
                     /locus_tag="VQ620_000027"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HET2762734.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            complement(24216..25382)
                     /gene="aroC"
                     /locus_tag="VQ620_000028"
     CDS             complement(24216..25382)
                     /gene="aroC"
                     /locus_tag="VQ620_000028"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET2762735.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            complement(25392..26459)
                     /locus_tag="VQ620_000029"
     CDS             complement(25392..26459)
                     /locus_tag="VQ620_000029"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET2762736.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKTRGNTLKLVLVS
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            complement(26478..27332)
                     /locus_tag="VQ620_000030"
     CDS             complement(26478..27332)
                     /locus_tag="VQ620_000030"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET2762737.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            complement(27322..27999)
                     /locus_tag="VQ620_000031"
     CDS             complement(27322..27999)
                     /locus_tag="VQ620_000031"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HET2762738.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            complement(27996..29159)
                     /locus_tag="VQ620_000032"
     CDS             complement(27996..29159)
                     /locus_tag="VQ620_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET2762739.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRSAYYQDDLTTAYRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASHTTSVDLAKRSRKLSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            complement(29843..31477)
                     /locus_tag="VQ620_000033"
     CDS             complement(29843..31477)
                     /locus_tag="VQ620_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2762740.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEIPIYFSLMVAILLVIAFSTSLTAFYNVFYESK
                     DLVSYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTTVFRKYQSIFSNVMIGIGVLIPLIFVFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSIEAILGLLAWIALTVFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPILTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(31458..32207)
                     /locus_tag="VQ620_000034"
     CDS             complement(31458..32207)
                     /locus_tag="VQ620_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2762741.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGKVED
                     LRKDHPDQSLESIYLSLAGRKEEVADASQGH"
     gene            32386..33840
                     /locus_tag="VQ620_000035"
     CDS             32386..33840
                     /locus_tag="VQ620_000035"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HET2762742.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPKVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(33930..36548)
                     /gene="alaS"
                     /locus_tag="VQ620_000036"
     CDS             complement(33930..36548)
                     /gene="alaS"
                     /locus_tag="VQ620_000036"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET2762743.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMLIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNHNASQLSSKLVAIVADNAEVEAVSEGTTSLIFAETPFYAEMGGQVA
                     DHGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENTELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQPKIQELLDAVAGKL"
     gene            complement(36570..37055)
                     /locus_tag="VQ620_000037"
     CDS             complement(36570..37055)
                     /locus_tag="VQ620_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HET2762744.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(37330..37569)
                     /locus_tag="VQ620_000038"
     CDS             complement(37330..37569)
                     /locus_tag="VQ620_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2762745.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(37822..38892)
                     /locus_tag="VQ620_000039"
     CDS             complement(37822..38892)
                     /locus_tag="VQ620_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2762746.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(38889..39662)
                     /locus_tag="VQ620_000040"
     CDS             complement(38889..39662)
                     /locus_tag="VQ620_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2762747.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKLSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(39659..40465)
                     /locus_tag="VQ620_000041"
     CDS             complement(39659..40465)
                     /locus_tag="VQ620_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2762748.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(40446..41603)
                     /locus_tag="VQ620_000042"
     CDS             complement(40446..41603)
                     /locus_tag="VQ620_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2762749.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(41763..42668)
                     /gene="murB"
                     /locus_tag="VQ620_000043"
     CDS             complement(41763..42668)
                     /gene="murB"
                     /locus_tag="VQ620_000043"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET2762750.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(42780..43532)
                     /locus_tag="VQ620_000044"
                     /pseudo
     CDS             complement(42780..43532)
                     /locus_tag="VQ620_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
     gene            complement(43542..44273)
                     /gene="budA"
                     /locus_tag="VQ620_000045"
     CDS             complement(43542..44273)
                     /gene="budA"
                     /locus_tag="VQ620_000045"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HET2762751.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            44415..45686
                     /locus_tag="VQ620_000046"
     CDS             44415..45686
                     /locus_tag="VQ620_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET2762752.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(45706..46521)
                     /locus_tag="VQ620_000047"
     CDS             complement(45706..46521)
                     /locus_tag="VQ620_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2762753.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(46678..47331)
                     /gene="phoU"
                     /locus_tag="VQ620_000048"
     CDS             complement(46678..47331)
                     /gene="phoU"
                     /locus_tag="VQ620_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET2762754.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(47343..48101)
                     /gene="pstB"
                     /locus_tag="VQ620_000049"
     CDS             complement(47343..48101)
                     /gene="pstB"
                     /locus_tag="VQ620_000049"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2762755.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(48114..48917)
                     /gene="pstB"
                     /locus_tag="VQ620_000050"
     CDS             complement(48114..48917)
                     /gene="pstB"
                     /locus_tag="VQ620_000050"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2762756.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKDFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(48928..49812)
                     /gene="pstA"
                     /locus_tag="VQ620_000051"
     CDS             complement(48928..49812)
                     /gene="pstA"
                     /locus_tag="VQ620_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET2762757.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(49802..50719)
                     /gene="pstC"
                     /locus_tag="VQ620_000052"
     CDS             complement(49802..50719)
                     /gene="pstC"
                     /locus_tag="VQ620_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET2762758.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNLSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(50719..51597)
                     /gene="pstS"
                     /locus_tag="VQ620_000053"
     CDS             complement(50719..51597)
                     /gene="pstS"
                     /locus_tag="VQ620_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET2762759.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(52171..53475)
                     /locus_tag="VQ620_000054"
     CDS             complement(52171..53475)
                     /locus_tag="VQ620_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HET2762760.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTLWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLISNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGHLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYASGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(53476..54249)
                     /locus_tag="VQ620_000055"
     CDS             complement(53476..54249)
                     /locus_tag="VQ620_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET2762761.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILFRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(54239..54517)
                     /locus_tag="VQ620_000056"
     CDS             complement(54239..54517)
                     /locus_tag="VQ620_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HET2762762.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(54517..54918)
                     /locus_tag="VQ620_000057"
     CDS             complement(54517..54918)
                     /locus_tag="VQ620_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HET2762763.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(55128..55745)
                     /locus_tag="VQ620_000058"
     CDS             complement(55128..55745)
                     /locus_tag="VQ620_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HET2762764.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(55735..56508)
                     /locus_tag="VQ620_000059"
     CDS             complement(55735..56508)
                     /locus_tag="VQ620_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HET2762765.1"
                     /translation="MKYKGYLIDLDGTIYKGKDQIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(56519..57256)
                     /locus_tag="VQ620_000060"
     CDS             complement(56519..57256)
                     /locus_tag="VQ620_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HET2762766.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEESISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(57261..58391)
                     /locus_tag="VQ620_000061"
     CDS             complement(57261..58391)
                     /locus_tag="VQ620_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HET2762767.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(58475..58864)
                     /locus_tag="VQ620_000062"
     CDS             complement(58475..58864)
                     /locus_tag="VQ620_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET2762768.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(58880..59263)
                     /locus_tag="VQ620_000063"
     CDS             complement(58880..59263)
                     /locus_tag="VQ620_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HET2762769.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(59264..60052)
                     /locus_tag="VQ620_000064"
     CDS             complement(59264..60052)
                     /locus_tag="VQ620_000064"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET2762770.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(60045..60980)
                     /locus_tag="VQ620_000065"
     CDS             complement(60045..60980)
                     /locus_tag="VQ620_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HET2762771.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLSETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(61110..61286)
                     /locus_tag="VQ620_000066"
     CDS             complement(61110..61286)
                     /locus_tag="VQ620_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET2762772.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(61426..62133)
                     /locus_tag="VQ620_000067"
     CDS             complement(61426..62133)
                     /locus_tag="VQ620_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HET2762773.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            62284..63312
                     /gene="queA"
                     /locus_tag="VQ620_000068"
     CDS             62284..63312
                     /gene="queA"
                     /locus_tag="VQ620_000068"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET2762774.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIINMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDSMFIY"
     gene            complement(63983..64372)
                     /locus_tag="VQ620_000069"
     CDS             complement(63983..64372)
                     /locus_tag="VQ620_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2762775.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(64426..65109)
                     /locus_tag="VQ620_000070"
     CDS             complement(64426..65109)
                     /locus_tag="VQ620_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2762776.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGGIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEYGYVTTTRTYDVNPETGHVTEQVTVD
                     RKQATDTVIKVPAKSKVEEVLVPCATKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDDPTLDKSQEVGEIG
                     KLLLLQSIL"
     gene            complement(65824..66375)
                     /locus_tag="VQ620_000071"
     CDS             complement(65824..66375)
                     /locus_tag="VQ620_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2762777.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDIEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(66773..67597)
                     /gene="nadE"
                     /locus_tag="VQ620_000072"
     CDS             complement(66773..67597)
                     /gene="nadE"
                     /locus_tag="VQ620_000072"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HET2762778.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(67594..69054)
                     /locus_tag="VQ620_000073"
     CDS             complement(67594..69054)
                     /locus_tag="VQ620_000073"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET2762779.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(69170..69658)
                     /locus_tag="VQ620_000074"
     CDS             complement(69170..69658)
                     /locus_tag="VQ620_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2762780.1"
                     /translation="MKNRFYYYQLLDEREEQLINKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(69648..69857)
                     /locus_tag="VQ620_000075"
     CDS             complement(69648..69857)
                     /locus_tag="VQ620_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2762781.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(70247..70689)
                     /locus_tag="VQ620_000076"
                     /pseudo
     CDS             complement(70247..70689)
                     /locus_tag="VQ620_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640911.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(70981..71787)
                     /locus_tag="VQ620_000077"
     CDS             complement(70981..71787)
                     /locus_tag="VQ620_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2762782.1"
                     /translation="MILKDINFALNKGEIVGLVGRNGVGKSTLMKILVQNNQPTSGNI
                     ISSDNVGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQERFRSLIQELDLTQSINKKV
                     KTYSLGTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLAVLKKLALNENVGILI
                     SSHKLEDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGD
                     IVQEEGLRITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNRSK"
     gene            complement(72193..72921)
                     /locus_tag="VQ620_000078"
                     /pseudo
     CDS             complement(72193..72921)
                     /locus_tag="VQ620_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(73157..73468)
                     /locus_tag="VQ620_000079"
     CDS             complement(73157..73468)
                     /locus_tag="VQ620_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2762783.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYVCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(73483..74769)
                     /locus_tag="VQ620_000080"
     CDS             complement(73483..74769)
                     /locus_tag="VQ620_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2762784.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMKELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     KGEILEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESSEKFEAIKQNLVDEMWKVAQRELATGFYYGTPSENEQLFGARRKIPEYKFVAEVV
                     SYDDATQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVKAGDMVRALKEGLINLYKEDGTSVTVRA"
     gene            75393..75575
                     /locus_tag="VQ620_000081"
     CDS             75393..75575
                     /locus_tag="VQ620_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET2762785.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            75585..75950
                     /locus_tag="VQ620_000082"
     CDS             75585..75950
                     /locus_tag="VQ620_000082"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET2762786.1"
                     /translation="MPNSENTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
     gene            75947..77137
                     /locus_tag="VQ620_000083"
     CDS             75947..77137
                     /locus_tag="VQ620_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET2762787.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
                     RNGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSS
                     NLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNEC
                     LNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
                     QQLL"
     gene            complement(77652..78644)
                     /locus_tag="VQ620_000084"
     CDS             complement(77652..78644)
                     /locus_tag="VQ620_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2762788.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
     gene            78804..80558
                     /locus_tag="VQ620_000085"
     CDS             78804..80558
                     /locus_tag="VQ620_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2762789.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAVLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIVTPILVLVLGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAEVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAIIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            80542..82263
                     /locus_tag="VQ620_000086"
     CDS             80542..82263
                     /locus_tag="VQ620_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2762790.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERIHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            82274..82903
                     /locus_tag="VQ620_000087"
     CDS             82274..82903
                     /locus_tag="VQ620_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET2762791.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALIALGAFLGGILGAYIGKALLKKHFSNGLYCVGYFTPCLILWCYLN"
     gene            82804..83361
                     /locus_tag="VQ620_000088"
     CDS             82804..83361
                     /locus_tag="VQ620_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2762792.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGINFKNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            83349..84713
                     /locus_tag="VQ620_000089"
     CDS             83349..84713
                     /locus_tag="VQ620_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2762793.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGSGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDILEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDESSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAI
                     ASVMCKTSPFIFFDEPSSGIDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADE
                     IFEL"
BASE COUNT        26155 a        18128 c        15516 g        25296 t
ORIGIN      
        1 tctctccacc agtaaaatgc aagcaattat acaatgttgt cacatagaaa ataatgtttc
       61 cgtaactttt caaagtaact tccatctctc tcccaaaact ggaagttagt tttagaagtt
      121 acctaaaaat caggtcacct attttaaaaa agcagcaaac tataaactag taggttccac
      181 accaaatgta gtcccatact gccccataag tcagatttat agcgcaccat acctaaaaac
      241 atcccaagtg aaacatacaa acaccaagct agaatggttc ctgtatgatg tgctaaggca
      301 aataaaacac ttgtcaaagc aactctgata tctaattttc tgaccaaatt ccataaaatt
      361 tctcgataca gaaattcttc aaccatactc gcattgatta agaacaataa aaatgaaaac
      421 caaggaattt gatgttgaag gccaattaag tttgcttgat tcgtgcttcc ttgagcatga
      481 atcagactaa aacatagact tataatcagt aggctaacaa attcaacacc aagccatttc
      541 atcctagatt tcatattgac cttatgcgct tgtttgcgtt ggccatacat ccataaaaaa
      601 gaaatgagtg acgaaccata gagaatctgt agtatagtta actcaccgat acaaagaaat
      661 ttcaataagt atagagatac caataggaca tttacttgtt ggaatatata aactggaatt
      721 attcttttca tagttacctc cgaaataaat cttcataatc taaatctaat acctgcacaa
      781 tcctttctac ccatggactt tgaggcattc attgttccat cttctagtgg cgaatctttt
      841 gatacaaacg attcaattca cttggatagt gaaactctcc cgcaaacatt tttctggtta
      901 actcaatcca gctgatattt ctttcagcca aaataatgga caagttctcc caaaatcgtt
      961 cagccatatt gcttctcctt tagttagata aataatgtgt ttgcgccatg taaatcaatt
     1021 gtttcgtatc tcttggcaat agagctctag cctcttccaa attcagactt ggataaactc
     1081 gcttatttga aaccgcaaga ggaagtctga tggttagttc aggatttttt aaaattatct
     1141 caacgaaatc cgttaatctt agattgtcac ggttcttaaa tcgtaataaa ttgggagata
     1201 aaaactcaaa acaatctgaa gaatagctca tcatctcaat taatttgtcc tttgtcattt
     1261 cagaaactga atgacaagat acctctatgc catagttttg gaagaaatct aaaagaagtt
     1321 gatttctttg tctattttta cttagataga gatcaatcat gggagacctc ccaaagattc
     1381 ggttccattt gatattctga cacgattaag gaatctaata aattaaggaa tctaataaat
     1441 ttgcgaagtt aatcggtttc ttgtcttcat cataagcttt tacagttact tgggttgtaa
     1501 gtattccctc ttttccctcg gctcgatagc cttgtccata taaaacaaaa acgagatttt
     1561 gatgatcatc tacaaaggca tcaaccccat tctttatgtc ttgactttca aggaattcca
     1621 taacgttttg aagataggat tcgtaaaata gtgggtagtt atgtttttta tggtaatcat
     1681 ctaaaaatgt cacttcaaac tcacatggag agtaattttg actttgaaca gcctaaaagt
     1741 gccatcaaat ttgaattgga ataaatcaaa taaatagccc catcctcatc aatccaacct
     1801 ttgctcaaag acaactccaa ccgatctttt aaaactgagt aaaccacctt aacctccagt
     1861 ttcatattct tataccgttc actctcaaat aaaagtttgg ggagcttata ataacgctct
     1921 gatgtctgat attgattagc ggtaatacac ttcattattg tccctccaag actaaaattc
     1981 caacatttcc aaattcatca aatcggatta aacctacttg ttccatttca tcaactaact
     2041 gagttgcttt tacaatatca actcccatga tagtcatgag atgactcttc acgaattgac
     2101 gtgatgactg tccttccttt tgcataatta cctccgaaac acaaaaaaag gggtagacaa
     2161 tctagtgtct acccccgaaa gtttattaaa acaaaaatcc tgccaaagaa tttttggcag
     2221 gaaaccaaat caatttatca gtttctatca atcgcttatc gctctcaaag actggtaaat
     2281 agggattccg caatcaaatt gcgatactct attatttaag agtaactgaa gctccagctt
     2341 cttccaattt agttttgatt tcttcagctt ctgcagttgc aacgccttct ttaacaagtg
     2401 ctggtgcacc gtcaacaagt tctttagctt ctttaagacc aagaccagtg atttcacgta
     2461 caactttgat aacgccaact tttttgtcgc ctgcagatgt caattcaacg tcgaatgaat
     2521 ctttagcagc accagcatca gctgcatcag ctgcagcaac agctacagga gcagctgcag
     2581 ttacaccaaa ttcttcttcg atagctttta caaggtcgtt caattcaagg attgaagctt
     2641 ctttaatttc agcaataatg ttttcaatgt tcaatgccat tgttatttcc tccaaataag
     2701 ttttaaattt tataatagtt tttttcgtag ctaggctacg ctgtgtagct taagattaag
     2761 ccgcgtcttc tttgctttct gcaaccgctt tgactgcaag agcaacgttg cgcactggcg
     2821 cttgaagtac agaaaggagc atagaaagaa gtccttcgcg gtttggaaga gttgcaagtg
     2881 caagaatctc ttctttagat gcgacagcgc cttcgattgc accaccttta atttcaagtg
     2941 cttcagcgtt tttagaaaag tcgttcaaga ttttcgctgg tgcgataaca tcttcattag
     3001 aaaatgctac tgcagatggt ccaacaaata cagatgcaag atcttcaaga ccagcttttt
     3061 cagctgcacg acgcaagatt gagtttttaa taactttata ctcaacttcg cttccacgaa
     3121 gctcgcgacg aagaactgta tcttgctcaa ctgtcaaacc acgagcgtct acaacgacga
     3181 tagatgcagc agctttcatt ttttcagcta ctacgtcaac tagttccgct tttttagcaa
     3241 taattgcttc actcattagt gtgttcacct ccgtaattat tttgcttggg gaatttttca
     3301 aaaagaaaaa cgcgcccaaa cctagacacg aaagtacaat acgcttcttt ttacatgata
     3361 cgtcttgtcc tcggtaggat atttatgagt cgagctcccc tactgtctta ggcagttttt
     3421 ttaaaccgta tataagtata gcatagtcaa aaaaagaatg caagattttt gcaaactttt
     3481 tttaaaattt ttcgtgattt ttcttttaaa gttctactgt caggacttga ccttgcttaa
     3541 caacctgttc tccggcgata taaacatcat ctacatcact agatttaact gcataaacca
     3601 ggtgagacag catattttcc tgaggttgga gatgaatttt cccttgtggt tgaatgacca
     3661 gaaaatctgc ttgcttgccg acttccagac ttcctatctg attttccatt ccaaggacct
     3721 tagccccttc gattgtcagt accttgagag ctgtttcgat tggaaactgg ctggcatccc
     3781 cacttttcat cttctgaaga agagctgcag tccttccttc ctcaaacata tctagattgt
     3841 tattggaagc aaccgagtca gtcgcaattc cgactgctac tcccgctttt tggatctgga
     3901 taattggagc aattcctgat gccagtttga ggttactgat aggattgtgg gcgatagcca
     3961 cttgagaaga tgccaagcgt tcaatttctc tctcgtttaa ttcgaccccg tgagcaaata
     4021 cggacggatg atctaaataa cccagttctt caagaaaagc aagggggcgt ttgccgtatc
     4081 gtttgaggat aattcctgac tcctccttgg tctccgccac atggacatgg agcggaatat
     4141 ttagctcttt tgccatttcc aaactcgctt ccagcaagtc tctactgcag ctatacggag
     4201 aatgaggtgc taccataacc ttgaaatttg gatttttata ttttaagatt tcgtctatga
     4261 tggatcgagt tctgcttata gtctcagcag ttgtttctgt ctctgaagaa aagagagtcg
     4321 gagaaaaata acaacgcatc ttggaagttt tcaccacctg ataaatttgc tggatatcca
     4381 caccattggg attatacata tcgttaaagg ttgttgttcc tgactggagc atctctgtca
     4441 gggcttcttt gaccgcattg gtagtcatgt cgggagtaaa ctcagattct gctggccaga
     4501 tatagtcatt gagccattca tggagattgc tgtcatctcg gatccctctc agacctgtca
     4561 ttgcagaatg ggtgtgacaa ttgaccaaac caggcataat ccaagctccc tgatagtcta
     4621 taatctgctc agcttgctct aaaaacgctg gcttatcttg accgacatag acgatttgag
     4681 aatccttgac tgctaagatt ccatcaagat aaacatggaa atcttgatca caagtcacga
     4741 tatttacatg ctgaaagact ttcattatag gctccttttc taaaagacaa gttgtactct
     4801 cttcaaacca cttcagattc actttgccgt aggtgtggtt actgacttcg tcagttctat
     4861 ctgcaacctc aaagctgtgc tttgagctga cttcgtcagt cttatctaca acctcaaagc
     4921 tgtgctttga gcaacctacg gctagcttcc tagtttgctc tttgattttc attgagtatt
     4981 acagtaaaca atttttctag ctattgtaac aaaaaagtca ctcgaaggca acttttttcg
     5041 tccataaact ttcaaaagag aatggcttat tcagaactaa aagccgcagc tttttgcatt
     5101 tggtaatact taccctttct atccatgagt tcttgatggt taccatattc aacaatatca
     5161 ccatctacta agacaagaat taaatccgca tcctgaatgg ttgacaaacg gtgagcaatg
     5221 atgaagcttg tgcggccctt catgagtttt gcaaaggcat cctgtaccag cacttctgtc
     5281 cgtgtatcaa tggaagaagt tgcctcgtct aagataagaa tctttggaat agccagaaag
     5341 actcgggata tggtcaagag ctgagcttgg ccgacagaga gagattctcc agcattttcc
     5401 aacttggtat cgtatccctg tggcaactgt tggatgaaaa agtctgcatt agctgctttg
     5461 gcagcagcaa ttacttgctc tcgactggct tcaggattgc caaaggcaat attatcatga
     5521 atggtccctt gtgtgagcca ggtttcttga agcaccatac caaactgctg tctcaatgat
     5581 actcgtgtat aatcataaat ggattgccca tccagcaaga tatctcccga gctaatggga
     5641 taaaaacgca taaggagatt gataagagtt gattttccag cacctgtcgg accaacgatg
     5701 gctaccttac taccagctgg aatatcgata gacaactcct taatcaaaat tttttcagga
     5761 tggtagccaa aagagacatg tttaaaggaa atagctccct taacttggtc actggtcaag
     5821 acttccttac ctgtttcagc cacttcaggg ctatctaaga ccccatagat acgctctacg
     5881 caagccagag cactttgcaa ctcagctagc actgaagaaa tatcgttaaa gggcttggtg
     5941 tattgctgaa catagttcaa aaaagtcact aaacgaccga cggtcaaggt tgaacccatc
     6001 atgatacgat aagctcctac tccagctaaa agggcataaa tgagtgcatt tacaaagcga
     6061 gtcgaaggat tgaccgttga agaataaaag atggctgact gagaatagcc tgagtagttg
     6121 tcatgagcct cacgcaatct ttggataaat tctgtttgag cattgaagga ctggattata
     6181 gtctgctggc taagcgattc ttcaatcaac tgagtctgaa ttcccctcgt ctctgtttgc
     6241 ttctggaaga gatgatagga tctcttggca ataaagcgtg aaatcaccat ggacagtggc
     6301 gtcaacagca agactaagag agtcatgagg agatgaattt ggagcatggc tagaatacta
     6361 accaaaatca tcaaaacacc aatgaaaaat tggttaaaaa tcatggtcaa gccagctgcc
     6421 aactgttcga tatccgtggt tacacgactg accatctctc cattgccttg ccgatccgca
     6481 aaagcaatcg gtaaccgatg gagtttatgg atgattcgct ctcgtaaatc tctggtataa
     6541 gagaagatta gacgattata gaggagagga ttggcccatt gtaccagagt atttcctatt
     6601 accaccaaga gcatctggag aaaaatctgc caaaaaactg gtgatgaacc agccactagg
     6661 acttggtcaa tgacctgccc aatcagaata ggtaggtaaa ttgataagcc aacttgggca
     6721 atagttccta gaaaggctag gaaaaggagg aagggatggt ttgctaaatc tacgactaaa
     6781 cgtttgagcg tctggtttac agtttgtcgt ttcattctag tcctcctttc catgttggga
     6841 tgcattgatt tcacgataga cttgactgga tttcatcaag tcatcgtgct tgccaacagc
     6901 tagcaactca cctttttcca agaggagaat ctggtccgcc atctggagtg tcgaagtccg
     6961 ttgagaaatc aagattaagc tcatatttgg cagattttcg cggatagctt tcaagagctt
     7021 ggactctgta atggtgtcca gtgctgaggt cgcatcatct aggatgagaa acggagcctg
     7081 gcgcaagact gctcgtgcga tagacagcct ttgtttttgt ccacctgaga aatttcgccc
     7141 tcctgcttca actagagcat ccaagagtcc ttccttatca ctgacaaaat ccttagcttg
     7201 cgcaatctcc aaggcttgcc agagttcttg atcagtcact tcttgattta aacctaaagt
     7261 caagttggaa cgaatagttc ccttaaagag ttcgaccttt tgaggtacat aggcaatcca
     7321 agaccgccac tgctccaaat taagaggact acgtccattt tgataaaggt caatgttccc
     7381 cttgtctact ggataaagtc caagtaagag ttgcaccaag cttgatttac cagaaccagt
     7441 ccccccaatg ataccaagga tttgtccttg agtcatatca aaggaaatgt ctctcagaga
     7501 aggctgggcc gcatcaggat aggtaaaagt caattcttgg acttgtaaaa cccgaccact
     7561 ggtaacttgc ttttgttcta attctgaatg gatgttttcg ggagattccg caaagacctc
     7621 ctcgattctc ttggctgaga tataggactg gttgagggaa ttgatcagca tggctagctt
     7681 gaccaattcc accaaaatct gtaagaggta attgataaga gcaatgagag caccttgact
     7741 gagtactcct ccttgaattg aaatatagcc ttgccagata ataacgagaa gagttccatt
     7801 gacaatcaga taggtcagag gtgttaataa actagacaag aaacctgtct tttcttgtaa
     7861 tctagcataa acttggttaa gggtttgaaa aatctgtaac tctcgttttt cttgaccaaa
     7921 agcacgaata acccgcatcc cttgcaattg ctggcgcgtt tcctgaacca gttggtccgt
     7981 tttctttctg agactactgt agagaggatt gaccaatcga gataacccta caatgacaat
     8041 ggtcaaaatg gcaaccatga ctaagaacca gaaagtcaac tcagctgaga ttcgataagc
     8101 cataaaaatg gcaccaaaaa cgataatggg cgctcgtaaa aagagacgca ggaattgatt
     8161 gataccagtc tgaatctggt aggtatccga agtcaagcga gtaaccaaac tagaagttgt
     8221 cagacggtct ctgctgtcct ttggcaagga aagaatatga cgataaagat cgtttgtcaa
     8281 ttccttagca aaacctactg ctgcctttgc tgagtaaaat tgagctatca aggccactaa
     8341 aacgccaatt actgcaaaga taaggagcag gccaatctgc atccagagat gaccttgatc
     8401 tccctgaggt aaagattggt caacaatccc agcaatcacc atgggaacca agagctcaaa
     8461 aacagcttct aacagcttga acaagggggc taaaattgat tccttgatgt agggtttgaa
     8521 gtaagataat aggtgtttca taaatccctt ctattcatat tctagaaatg aagaaagtgg
     8581 gaagccccac tctctgtttt atttgtttaa gtaaggcaat agatagccat atcctgcttt
     8641 ttccatctca tccttggcca caaagcgtaa agaagcagaa ttgatacagt aacggaggcc
     8701 gcctaactcc cgcggtccat ctgtgaaaac atgacccaag tgagcactgc ctgaacgaga
     8761 acgaacttca attcgctcca ttccatggct cagatccttg taataatgaa tcaactcttt
     8821 ggaaatcgga cggctaaaac ttggccaacc acaacctgaa gcaaacttat ccttggcaaa
     8881 aaagagtggc tcacctgtcg taatatctac ataaatcccc tcttcaaagg tttggtcata
     8941 ggcattggta aatggagcct ctgtagcagc ttcttgtgtg acacgataag actcttcaga
     9001 tagactggcc ttcaacacct cttgactagg cttttcatag tttgctgcat caatcaatgg
     9061 cttatcagca tcggtcacat cgatatgaca gtaacctgaa ggattcttcc tgagatagtc
     9121 ttggtggtag tcttcagcca gaatgtagtg gcgtaattgc tccacttcta ctgcaatctt
     9181 tcgacccagc atgcgttcct gctcctgcac cactgtgtag atagctggca aatctgcttc
     9241 atcctgataa taaatcccag ttcgatattg gcgaccacgg tcattccctt gttgattgat
     9301 agatagagga tcgataactc ggaaataata aagtaaaatc tctctgagtg acacttcctt
     9361 ctcatcgtaa atcacttgga ccgtttctgc atggtctgtt tccttgatca actggtaatt
     9421 ggtcgtttcg acttgaccat tagcgtagcc aacactggtt tctagcactc cagaaatgcg
     9481 tgaaaaatat tcctctaggc cccaaaaaca accacctgct agataaattt ctgccatctt
     9541 tatttctcct ttatcacgtt tttaactaat actcttcgaa aatctcttca aaccacgtca
     9601 gcgtcacctt accgtaggta tgttactgac ttcgtcagtt ctatctgcaa cctcaaaaca
     9661 gtgtcttgag ctgacttcgt cagttctatc tgcaacctca aaacagtgtt ttgagcagcc
     9721 tgcggctagc ttcctagttt gctctttgat tttcattgag tataaacttc ttttcacaaa
     9781 aaggatatga aaccctctgg tcaagagttt ccccatccta tcttctattc tttattttgc
     9841 ttctacacgg acacctttgg tatcaactct caagtcatgc agttttcctt tgaaaggttg
     9901 cttttccaat tctgccttaa ttgttggcat cttgtcatga gaagccagaa ccataactgt
     9961 cggcccagca ccagaaaggt aggttgcata ggccccattt tctttggtca cttgcttaat
    10021 catcgcaaat tctcttacca agtcctgacg atagcgctca tggaagaggt ctccctcgat
    10081 tgcttgccca gcggtcacca tgtctcctgc caacaaggca gcaaccgcta cattggcgat
    10141 agaacttgca gcaacagctt ccttataaga caatttttta ggcaagacac tacggctgtc
    10201 gcgagtacgt aattcatagt ttggaatgta agctagaaaa tcacactctg gaaagtctgc
    10261 tacgatagca gagacttgcc cttcaacaga acttgcaata acgagattac cataaatggc
    10321 tggagccaca ttgtcaggat gcccttcaat cttggtcgct aactgcaatt tttcatggtc
    10381 tgataagttg agttgaccca gttggttggc tagttcaatc ccagcaacga taaccgagct
    10441 ggaagaaccc aaaccgcgcg ccaaagggac atcactggtc attttcaagc gtcttggttg
    10501 caagtctggt acaatttgca aagcgatttt aagcaagaga ttacgctcgt catgtggaat
    10561 ccatttgcca atctggtgtt caatcagcca ctcatctcgt tcttcgcaga cctcaatttg
    10621 aagatacttg gttacagcta caccgaccga gtcaaaacct ggcccgatat tggcactggt
    10681 tgcaggtaca ataatcttca tcttattctc ctagcacctt gaaggtattc aagaggtcga
    10741 attctgaaac cttcttcaat tcagctgaga cattttcaag ctgggcttta ttaatcttgt
    10801 gtgtgatgat aacgacacgc gccttgtcac cctctttgcc atcttgaagg atttgcttaa
    10861 aggaaatatc ttgagcattg aagatttcag ccaacttcaa gacctgacct tttgagtcta
    10921 gagccaagat tgagaaatag tagtttgctt tgacatcttc aggatttgcc aagaccaagt
    10981 cacggctata ttcgttgaag tctttgccaa tagtaccatc attcaaacga cgaacgatac
    11041 ggacaatatc agctacaaca cttgttgcag ttggtttttg acccgcacct ggtccgtagt
    11101 acatagactc accaataccg atagattcta caaagacagc gttcattacg ccattcacac
    11161 tagcaagtgg gtgcgcttta ggtaggaagg ttggagtcac ttctgcagca atacctgaag
    11221 aagtttcctc aatagaacca accaatttca ctacgtaacc aagctcttga gctacagcta
    11281 cgtcttctgg tgtgatattg cggattccct tgtgggctac atcatcaaag gcaatcttca
    11341 tgccaaaggc aaattggctc aaaataacca tcttgtaggc tgcatcaatc ccatctacgt
    11401 cattcgtcgg atcgctttct gcaaatccta gacgttgtgc ttccgcaaga gcatcatcgt
    11461 aagaccagcc ttcttccacc atcttggtca ccatgaagtt ggaagttccg ttgactactc
    11521 caagcacgcg cgtaatttta tcagaagcca aggaatttgc taaagtacga agaattggaa
    11581 tcccaccagc aactgctgct tcgtagtaaa gtgctacctt gttagcttga gcgatttcta
    11641 gcaattctgc gccatggaca gctaaaaggt ccttgttagc agtaacaacg tgttttccag
    11701 cttccaaggc acgagtgata aaggttttag caggctcaat acgccccatc aattccacta
    11761 cgatagtaat atcctggtct gataaaatat catccacatt ggttacaaag ttaaagtcat
    11821 tccctgctgc aagcaagcga tttttttcat cttcatcctt gaccaatacc ttagcaactt
    11881 tgatatctga atgtgctgat tgattgattt ttcctccatt ttcctttagg aggaaaggca
    11941 caccacttgc aacggtacca aatcctagta aagcaatttt aactgtcatc tttatctcct
    12001 cgaaattttc taatatagcc attataacag aattttgtga aaattcctat tatagtaaat
    12061 cactatttca gtataaaaag aaaaaacgaa tcagacgatt cgctcttctt aaaatctgaa
    12121 aatagctttc cagaaaagat tagccgattt tttgcagatt gagcactgca tcgtgactca
    12181 tcaagacttg accatactct tgtaagactg agcgactgat atcactatcg tctgcaaact
    12241 cgcgcatacg ggccaacagc caagctggat atgggcttgg atgattttca atatccacta
    12301 aaatggtcaa ataatagcgc tcgttcattt tgtagagttc agaagtttcc atttcaaaag
    12361 tcactgtctt ggcaaaagct accaagtcag ccaacttagc aaaagaaagg atgtagtaga
    12421 tgtaaggttc tttcttactc tcagcttctt gttcagcctg ctcttgctct tcttccttgg
    12481 cttcaacttg ctcaaaagat tgaatggctt cgatatcatc cttggttttg tctgcgatgc
    12541 ttttttccag ggttttgata aattcatctg gagacatttg agccaattct tccatatctg
    12601 gcaaatccga taagtcttca aaatctagat tttggtcaat ctttgacttg gtcacaaaga
    12661 catctacctt atcaggtttt ggagtcacac ggaagctcaa catgcctgta tccagaaagc
    12721 tatcaggcat ctctagctca tccaagatag cataaaagaa ctcttctgtt ttttcttgag
    12781 gaacgagaaa gtcagcaatc tccattccac gatccatcaa atcctctaaa gacatcgtga
    12841 tttttaaagt tgtatcacta atttgtttca ttttcattgc tagtaacctc atactttcag
    12901 ttctatctat tatactagat ttttacgatt ttatcaaaag aaggctcctc tatacggata
    12961 gattttccct agggtctttc tataggagac tccaagagaa aatttctgca gacagataga
    13021 aaaagccttc aaaatcggct aagagccgat tttgaagacc ttatacatca gaatacttat
    13081 aatttaaagg ttgctacacc gaggatagaa cgatttaagt ttctgagaat ttgaagactt
    13141 tgctcaaatt tcttataacg agtcactccg tactcttcaa caagaaggac tgtatctctt
    13201 tccaaaagag atgatacatc ctgtaaatct acaaaatgca ttccttttaa agcttcttga
    13261 ctctgtttca atttatctag gatagcttta tttgagctaa cgatggtcaa ttcctgtcca
    13321 gtatttttgt atgacaaaac atctgctagg ttagcaattg ttgtaatctc tgttacaaaa
    13381 tcaatttgat actgagaaaa atcacctact ctattgattg ttggattaaa gagataaact
    13441 aacacatttc ccatcacaac caaaatcaca caaaccactc caataacaac taaacgaaga
    13501 atcagatttt tcacatttaa gccaagcgct gtttcaccat ttgcgttcaa ttctttagag
    13561 ttgatggttt ccagtttttc aattttcaca tttgcatagg catgtttaaa tttctcaatc
    13621 aacccatcaa tttttttctc taacaagtca ttggcatctt tacttgatgt caaaattttc
    13681 acaccaaccc ctgcatcgtc aatcatatag tagacggtca attttttcca ccaatagtca
    13741 ttcgttgaat ttttcaaggt tgtttctgtc gtgtctaatt cactggcaat ttttttcaac
    13801 tcactgggtt ctacatcatt gaaaagataa gctccattca aattaccatc aatcaatttc
    13861 ccataaaaat cactataacc accaatttga tgattcaaaa tcgttttggc cgactctttt
    13921 ggaggagtga ttttatagat aagataagtt gaataacttg ttgtatcttt gacagtgttt
    13981 ttattcctaa ctgctttaat tgtaaatggt acagcaatga gagcaaataa agcgatgaga
    14041 gctaaaatat ttgcttttcg ctttttataa agatttgcaa acaaatcagc tactgaataa
    14101 tgttcaaaca tgattttttt ctcctttgtt tagtagatac tagttttcct ttgtaagcat
    14161 ttttgctata aatataatca caagaacaat tccccagaat tgcattgtaa ataaattgaa
    14221 gaaactttct gaaaagctgc ttcttggcat aaagaataga ttattcaaga tgagtaggga
    14281 taaagcaaat aggattgtcc ttgagcgata ggctacttgc agcatggcta taaataatac
    14341 gccgagtaag aaactaagca gaaagactcc aatcatacca tagtcggtat acaactccat
    14401 gatataacta cttccgatac catgcccttt caggtattcc ttgttcaaga caagatagga
    14461 tagattgtgg gcataactat tactatcaat agctagttcc acactattgg ttgtatgttc
    14521 aaaggctttt cctccgaaaa tggctcccaa actccccctt gcaaaataat caagaacagg
    14581 accaaaagta aaattacgga aatctcggta agggaggcta ctgttaaata gaaaacctcg
    14641 agccagaaca ccaaaactag tcccttgttt atagataaag tcaagtaaga tatcccagaa
    14701 acctgtatgg gaaacttgga cattatcccg tacataattg agtactccca tcgctaacat
    14761 gagaatagga gaacctacaa aaatcgctaa cttttcttta aacccaatcc attttccttt
    14821 ttcagtttgc tcccgcataa agtaataaac aaaagcaaat aaaatactta aaataaaggg
    14881 atttcgtgtc ccaattgcca aatgaatagt attagctgca ataaaggaga caagcactgc
    14941 tgtggcctgc aatttctttg gcttggttgc cagatacata cacattgcat agaccgtaaa
    15001 ggtagacaaa atgtaggtaa aataaggcag tttactttca aaatttgcat agtaggcata
    15061 gtaggaagtc tgcaaacgat acaagagccg ttcaaataac cgaatgaaat agaaaggata
    15121 agttagaaga aaaactccta gtgatacaaa gcgtaaccgc ttgatataaa cctcttttag
    15181 agaatttcct atatttgcta cttttatttt cttcctagct atgaagtaac gagccagaat
    15241 gcctcctgtg gtcaagccca gaatcgaaac catgacaact ataaaggcaa aacgataggc
    15301 tattggatga taggtatcca aagcaccatc cctaaaataa tcaatggtcg gtcttgatac
    15361 cagaaataca aaaatggtta aatagaaaat aaaatggatt aagtaatact tgatatcatt
    15421 ccaacaagca attaagctac taaccaacaa gaacaataaa gtagaaagta agctaacatt
    15481 attattatta aacagataca caattccact tactagcgtc aaggcataac tgactatggt
    15541 caaactaaat aataatcgtt tcccatcaat cacttggtca cccccgttct aatgtaattt
    15601 tttagatttt tcaatatttt tcagtaataa gaatcgatat aaggaaatat ttatgaatag
    15661 ggccaaagca ctaattcttc tccccttacg gaaaattgga ttcctagaaa tagcaaaggc
    15721 atggcctttt aaaaaacgat gaatctgaga ataggcttca aactgtttat actgatcatc
    15781 tagcaacatc ttatccagaa taaagaagtg ggcataggcc aatctgaaaa aagcgacctc
    15841 tttcaagtca ggatagtttt tcacaacttc attataaaac ttttggtaga tatcaatata
    15901 ggctaaatcc ttctctgcat agggtttggt cgtaatacta tcccctctat ggaaatagta
    15961 ataatagggt ttagtattaa ccacatactt cttggccaac ttgattaaat caaaatggta
    16021 ataggcatct tcgtaaatca accccttagg aaaggatagg gcagttgcaa tctgtctctt
    16081 gattagctta ttgcaaatcg tcccaggtat tttttcacct atgaggtatt cctttagaaa
    16141 tgtttgagaa tcacagacaa aatagtcatc ctgattggct gactgtgggc tttcatcatt
    16201 agcatagaca ttcatgacac cacagcttga aacatccgca tcttcttgaa ctaattgctc
    16261 atataagctc tgaatcattt ctggatggat ataatcatct gagtcaataa aaatcagata
    16321 atccccgtga gcctgcttca tcccatcatt tcgtgcttgc gacaatcctt cgttcttttt
    16381 atgaagcact gacaccctgt catcttgttc agcgattgag tcacacaagc gaccactttc
    16441 atctgttgca ccatcatcaa caagaataat ttccagattt tgatagatct gcttctgaat
    16501 ggaagctatc gatttttcta ggtactgcgc cacattatag actggcacaa tcacactaat
    16561 taatgcagtt tccatgctac tcctctaata gtttttctac ttgttcgatt tgttttgtaa
    16621 ttgtaaattg ttgaatgaat tggctagcct catcgacatc aaagtttgag gcagaagtca
    16681 tgtaattagt aatcgcctga gctgcctctt gattgctctc aatgatttgt ccaaatcgtc
    16741 cttcttggga taattcctca gcccctccaa cgtccgtaga gataaaaggg agtcccagac
    16801 tcaaggcctc cacatacact ccaggaaaac cttcttgttt agacatagac aagagaactt
    16861 tcgtctgaga taaatactga taaggatttt tttgataacc aaggaaatgt acatagtcct
    16921 caatcccata ctctttgact cgttttttca gttcctcttc catatcacca gccccgataa
    16981 aatagagatg atagtttttt ccctcttggt gtaataatcg tatcacttcc actacacggt
    17041 cagaaccctt attttcctca atccgtccga tagtacagat actttgagga gcaatctcga
    17101 tatcgatctt ctcttgagat ttttctagaa tagtctgaaa atcatatcca ttgtagatta
    17161 tctgtaattt agaagcataa tctggataaa cttccttgat agaattgctg gtcttttttg
    17221 aaatccctac aattgtattc gcagcatcca actggcttct atgtgattct cttttagagc
    17281 tatccttaag aagttcttca atacttccat gaatccaaga tatcttcttg acttctcttc
    17341 ttttagagaa caacagtggt ggattcataa tggtaaaaga aacttcaaca tcataatcat
    17401 cttttacaag caaacgacga gtcagtcttg gaaaataaat tctcattctc cacaaaaaag
    17461 ctcgtaacca tctggtttgg cgataatctt gaagggattt taaaatgcgt acatgctttg
    17521 gaacagattc atatcccttg tcaaagtgct ccatttcaag aatatcaata tcatactttt
    17581 ctagatccag atttgaaaca atggttgata gaatcttctc tgcaccacct ccaagagaaa
    17641 aagaccacat aaaaaataag atttttttct tagccaccat attctccctt gtattctgta
    17701 taagacttat ccatatcagc gatgacagca tcatgatgcg gtacctgctt gtctgctggt
    17761 ggaggcgtca tataatcccc aaaagcagtt ctgagataga catcatagcc gattggaata
    17821 ggcatctctg ttccttcaaa tggcaagaaa agattgtctt caaaagatgt gattgggtac
    17881 ttgtttctca tgtagccagg acctgagcat aattctgtaa tgccatcaca atcagccaaa
    17941 tcatacttag tcatttcttt ctcagctttt ttccagatgc gataacggag agattttgga
    18001 gtcaaaccca gtaaaatgcg acttccccat ttcatgagag caccatgctt ttctggaata
    18061 gtttgcgcac aaaagagtga ataaatcaag gcccaacgaa cctgtttttt ccgctcagct
    18121 ggatttttcg gataataatc caaaggcaaa acatccaagg tcagaccatg tggcaaatct
    18181 aaatcctgct gataaggctt gatacaggtg gtttccttgt cacgaatggt aataaaaaga
    18241 ttacgatcaa caaaatcctt gtgactcttt gacaagaaat aacgttcatc tgcataacga
    18301 ggccataatt ctgctaattt ctcataatct ttacgaggca taaaaaagtc taggtcgtcg
    18361 tcccaaggaa taaatccctt gtttcgaagg gcaccaatag cgcctccgcc acagagataa
    18421 cagagcaaat catgttcttt acaaaaggcc acaaaatatt cagccatctc cagactacga
    18481 gcctgaattg cttttaaatc agtcatattg ttcattattc tttctatcgt atcgtttcat
    18541 tataccacaa acaaggggtg aaaatctatt gcagactgta aaaaatcaaa gcctgactgc
    18601 tatccaaata gctatcaaac tttgattttt ctgtcttata ctcttcgaaa atctcttcaa
    18661 accacgtcag cttcaccttg ccgtaggtat aggtaactga cttcgtcagt cttatctaca
    18721 acctcaaaac tgtgttttta gcagcctgcg gctagcttcc tagtttgcac tttaattttc
    18781 attgagtatt atcttatctc aagcccattt gagcgagctt ggtttgatat ttgttttgat
    18841 caaccagcaa gcccaagcct ccataaacat cataggcatc tacccagtca cccagttctg
    18901 gaatcgtcaa tttttcaata ccattttttg ctccatccaa aacagataaa ccgtttgtta
    18961 ggaggaaagt atagggtacg ttggttgagg tcatagcaaa aacctttcca agagcttcag
    19021 aaccagtgaa aagtttagtg ggatctttaa tttgctctaa aattgctgtt aaaacttgtt
    19081 gctgtctttt tgtacggccg taatccgcct catcatcatc acggaaacga gcataattga
    19141 gcagggtcga gccattcatc tgctgttttc cgactttaat ggtttgggtt ggagactcag
    19201 tctcggtagc gtataaatca tctccgactg tagcttctgt taggggacgc ccattcaatg
    19261 ttgaaaattg agcatcaatc gtcaccccat cagggaaaag cgtgtcaatc gctgtggcaa
    19321 aggcctggaa atcaaccaag gcgtagtact taatgtccaa gtcaaaatta tctttcaaga
    19381 cttggcgaac catttctgcc cctttttgcc cctcttgttc tcctaactcg taggctacgt
    19441 ttaacttgtt atctgtctgt tttctaccat taatcacttg actataacca tctatataga
    19501 ccaaattatc acgcatgaaa ctgactagct tcattttctt atctgagccc ccgacattta
    19561 ataccataat agagtcagtt cgtgtctcaa cactgttctg gccgattcga ccatcagtac
    19621 ccatgattaa aatattaact ccatctctag tgtcctgacc attaaagact tctacttgag
    19681 ctgcccgggc atcagcagtt ttctttgcgc tagcatcttg gtaaccacgc aaaaacatga
    19741 ataccatggc caaagccaca cagaccaaaa gtgaaaaaat caccataaaa attcgtttaa
    19801 gacggagctt ccgtcttttc ttttttggag ggaaagagag tgcttgtgat ttggattgtg
    19861 agcgactccg gttcgcatag cttggtaagt caacctgctc ttctctttct tgttccaagc
    19921 tagagctact atttccccta gcaagagtta gcttttcttg caaataggca aactcatttt
    19981 tttctctctc attgagatag tgaatatttt ttagcaaata atcataacgc aactgctcat
    20041 gatgacttaa gggattttct ttactcatct tctctccttt ccatggtctg atattggata
    20101 aataggatag gcacccagaa ttttatactg gattccaatc gcttctaatt ctttttgggc
    20161 aaagtggacc aagtccttat cgatataatc cacatcgata atgaaaaagt attcacccag
    20221 tgctgtcttg agtggacgac tttcaatttt tgtcaagtca attcctcgcc aagcaaaagt
    20281 cgacagggcc ttataaagtg cacctggaag gttgtcaggt aatgtcaagg ccaaactcat
    20341 cttttcagtt tgtgcttgca agggaatact aggcttttca gctcctagaa cccagaaacg
    20401 tgtgaaattg gcttccattt cctgaatatc ctcggcaatc agttccaatc catattcttc
    20461 agcagaactt ctaggtgcaa ctgctgcaaa gggctggtct ggatgttcgg aaataaaacg
    20521 ggccgcataa gctgtactag ctgttacctc gatttgagcc tctggatatt gttcatcgat
    20581 gaatttcttt ccttgagcca aggcctgtgg atgtgaaaaa atcttttcaa tcttagtatg
    20641 gcctggaacc accatcaact gctgatgaat aggctgaacg atttctgcta ctgcttggat
    20701 gtgagcctga tgaaaaagat agtccaaggt ttcatgaaca ctaccctcaa tagaattttc
    20761 aactggcacc acagaatagt ccactaatcc ttgctcataa gccttgatga catctgtaat
    20821 gttggcaaaa gcctgcaatt cctcatgagg aaaagctgtc tgcacaacgt ggtgtgaaaa
    20881 tgatcccttg ggacctagat aagctatttt catcttagtt cctctataat ttcctctggg
    20941 cttagcttgg tcacatccaa aacccgacta gccacttcct cataccaagc ctgtctttct
    21001 tgaaaaatag ctactagttc ttccttgcta ttatttagaa aaagcggtcg ctgattgtcc
    21061 ttatcagctg cgatacgtag gtagagggtt tcaaaatctg ctttcaggta gatgttatct
    21121 gtattagtct tgagtaagtc acgatttctc tgagaaataa ccactcctcc tccagttgac
    21181 acgacttggt ctgtttgtag taaatcagct aggacttctg attctacatg acgaaaggct
    21241 gcttctccct tttcagcgaa aaaattcgca atggacatac ctaggcgctt ctcaatcaga
    21301 gcatccatat caaggtaatt agggtccaag cctcttgcaa tagtcgattt tccagccccc
    21361 ataaacccta ataacacctt agccatgaat caagctctcc aaatcatcaa agaaactagg
    21421 atagctggta ttgatggctt ctgcacggtc aagctccacc tctccatctg caaccaagag
    21481 ggctgcgata gctgtcatca tgccgatacg gtggtcacca aacgtattga ctctagcacc
    21541 gtgaagagct gatttccctt tgataatcat cccatctgcc gtaggagtaa tatctgctcc
    21601 catactattt aaggcgtctg ccacaacctg aatacggtct gtttccttga ccttgagctc
    21661 ctcagcatcc ttgataactg ttacaccttg ggcttgagtc gcaagcaggg caataatggg
    21721 caattcatca atcaatcgtg gaatcaaagc gccaccaatc tctgttcctt tcaagtcaga
    21781 agactcaaca atcaaggtag cagatttagc gactggatcg atttcagtta tttccaattt
    21841 tccacccatg gcacgaatga catcaataat accggtgcga gtttcgttga tccccacatt
    21901 ctgcagcact agacgagaat ttggagcaat caaacctgcg actaaccaaa aggctgcact
    21961 ggaaatatct cctggtacga ccaccttctg tcctgtcaat ttttgtggcc cctggactgt
    22021 gattttctta ccatccacac ttaaatgacc accaaattgt tgcaacatat cttcagtatg
    22081 attacgggtg tactcttttt cgataataac tgactccccc ttagcttgta aggctgcaaa
    22141 catcaaggct gacttgactt gggcagaggc aattggcaac tcataatgaa taggtcttag
    22201 gtttttcgtc ccttttaagc gaaggggagg caagtctcgt tcagtttgcc ctgaaatgct
    22261 gacgcccatt tttttcagtg gaagggtcac acggtccata ggacgtttgg aaagactatc
    22321 atctccaaac atctctactt cgaaatctgc accagcaagg acacctgaaa tcaggcgaat
    22381 cgaggtgcca gaatttccca tattaagggc attttgtggc gcttttaagc cagccatgcc
    22441 tacaccttga atggtaataa ccccatcttt atcctcaatt tcaacaccaa ggtcacgaaa
    22501 aacctgcatg gtcgaaagaa cgtcttcacc tcgcagaata tcataaacct tggtctcacc
    22561 ctcagccaaa cttccaaaga taatggaacg gtggctgata gacttgtcac ctgggacgcg
    22621 gatactacca tgtaaatggc gaatgtttgt ttttagtttc atactggacc tcatacttgc
    22681 aatactttta cctattttat cataaaaagt cagaaattcc ttaaaaattc ctgactttag
    22741 gatcgttctt ttcttatttc agcaattctg aaactggttc aaaaacaatt ttttcaatgt
    22801 cagaaaggta aatggccaat tgttgttgct tggtaaagaa ttctgacaag aggctatttc
    22861 cttgaatctg tttaccaaag ccttccatct tagcttggaa ggacgcatct ggcatttgac
    22921 ctgtctgtgc tagtttttga atttcctctt gaaaggcaag atattctgta aagattttgc
    22981 ttgcctcagc atctgctgca atcgcatctt tagctgcttt aacagccttg tattctggta
    23041 atccgcgtag accgcgactg agttcgtttg cactatcgta aatatttgac atgttcttct
    23101 ccttatttga tgacgactgt atagtcggta ttttctgtta tgagatgctc agctctttcc
    23161 aagtcttgag catttttaaa tgaaatttgt aggattccgt gaatatcctc acgattttcc
    23221 tcgttgatgt ggatgttaac caaggaagtt ccacgtagca gttccaaaat ccgcaggatg
    23281 acatcttctt catcaggaac gtcaacatag aggtcgtaag agctatccac accaccacgc
    23341 ttatggattt ccatggtctg gcgttgttca cgcgcttggt taaaaaagtt ccaaatttgc
    23401 tcttcatctc ccttactaat ggcctgacca atcacttcca aacgttcctt gaaatcctca
    23461 attctatcca gaatggtctc gctattggac aagagaatgg aggtccacat tcctggctcg
    23521 ctttccgcaa ttcgggtcat atctcgaaaa ccacctgccg caaagcgcct tgccatctca
    23581 tgctcttgag catagaccgc agtctgctcc atgagactag aagccaaaat atgaggaaaa
    23641 tggctaatct gagaagtgac acgatcatgc tccttggcat caatctcgat aaaacgagca
    23701 tgaagacctg aaagcagatc cttcatttcc ttaagcgtgt cctgacttgt caggcttgaa
    23761 ggtgtaaaga tataataggc attttcaaaa agattgacat ctgccgaagc agcccctgtc
    23821 ttgtgactac cagccatggg atgggccccg acaaagcgaa cagacttgcc agccaaatac
    23881 tgctccgccg catccacaat ggttgacttg gtcgaaccag catctgaaat aataacgcct
    23941 tctcgcaaat ccaaattggc caactcctta atgaaagcaa tagtttgttt gattggcaag
    24001 ctgaggataa tgacatctgc caaaggagca aaactagcaa aatcatccgt tgcacggtca
    24061 atcatacctt ctttcaaggc gatatctctc gaagcttgac tacgattata acctaaaatt
    24121 tcataatctg gatgatcgcg tttgatacca agtgccatag aggctccaat caacccaaga
    24181 cctgcgatat agattgtttt tgccatagga actccttaat agttctttgt atagtctcgg
    24241 tgtttggcta ccgcttcttt tagttcctca agattatctg atgagaattt ttcgaggatt
    24301 tcttgcgcca gaaccgttgc tacaactgct tccatgacca ttcctgcagc tggaagagca
    24361 gtcggatcac ttctctccac ggttgccttg taaggttcgt gggtttcgat atccacactc
    24421 ataagaggtt tataaagagt aggaatgggt ttcatgaccc cacgaacaac gatgggttgc
    24481 ccattagtca taccaccttc aaaaccacct agattattgg tacggcgagt ataaccgtct
    24541 tctttagacc agagaatttc atccataact tggctgcctt tacgataacc agcctcaaag
    24601 ccaagaccaa attccacccc tttaaaggca ttgatagaga caacagcttg agccaatctt
    24661 gcatccaatt ttctatccca ttggacatag gaaccaagac caactggaac gcctccgacg
    24721 actgtctcca caaccccacc gatggtatca ccatcacgtt tgatttggtc aatatagtcc
    24781 ttgatttcct gttctcgttc ttggttgaca atagaaactt cagactgggc agctctttgc
    24841 ttaatttcag cgactgtcag attttcagga acatctattt ccttaccacc aaagaccacg
    24901 acatggttgg caatctccat atccagctca gccaagaggc gtttggctac tgcaccaact
    24961 gccacccgca tggtggtttc acgagctgat gaacgctcca aagaatttcg caaatcatca
    25021 aaacggtact taatcccccc aaccaaatcg gcatgacctg ggcgaggatg agtaattttc
    25081 cgcttgcttt taaggcggtc ttcaatgtcc tccgcagaca tgatgtccag ccatttctgg
    25141 tggtccttat tgatgacatc catagtaata ggcgcccctg tcgtcttccc gtggcgaacg
    25201 cccgaagtaa agacaacctg gtcactctca atcttcatac gaccaccacg accgtagcca
    25261 ccctgacggc gtctaaggtc ctcattgata tcctcagctg tcaatggaag tccagctgga
    25321 attccctcaa taatagctgt tagacggggg ccgtgtgatt ctcctgcagt taaatatctc
    25381 atacactctc cttattttac caagtagtct ttcatctctt ccagagaaac tgggtgaatg
    25441 atcgctgaac caagctctga caccaagacc aatttcaagg tgttaccacg cgttttcttg
    25501 tcatgagtaa gagcctgata aagcttgtca acttcccaat tttcatagtc aacaggcaag
    25561 ccgaatttct gacacatctc tgtgatagat tgggtaatgc cagctggcat gaggcctttt
    25621 tcctcagcaa ccttggaaat ctgtaccatt cccatggcaa cagcctctcc atgcatgacc
    25681 ttgccataac cggcagtcgc ttcgatggca tggccaatag tgtggccaaa attgaggtaa
    25741 agacgaatac cattgtccaa ctcatcttca accaccatct tgcgcttcac ctgacaagaa
    25801 tgttcaatca aggtctctgc atgttccaaa atactctcaa cagaaccatt cagtcccgtc
    25861 aagagagccc acagttctgg atcctcaatc aagccatact tgataacttc acccatccct
    25921 tcaatcaact ctctttttcc gagggtttca agaacaagtg gatcaatcag aaccccatct
    25981 ggttgggcaa aggtccccac catattttta gcaaatggtg tgttaacgcc tgtctttcca
    26041 ccgatagaag aatcaacctg agctgtcaaa ctagtcggaa tctgaacaaa gtgaataccc
    26101 cgcatatagg tagaggctac aaagccagcc aggtccccaa caacgccacc accaagagca
    26161 acgattccat cgctacgagt cagaccttgc ttgactagaa attcatagac tttctgaaca
    26221 gtagttaaat tctttctttc ttcaccttct aagaaatcaa aaacagctac ctgaaaacca
    26281 gcatcttcta ggctgagctt gaccttctct gcatagagag aggctacatg gttatctgtc
    26341 acaatgacta ccttttgcgg ttgccagagt tctcgcaacc actgaccagc ctgggccata
    26401 caaccttttt caatctgaat atcataagga tgatgaggaa tatcgattct gattttcata
    26461 ggagagtctc cctttcttta ttggtatttt tctgttaaag actgccaaat ctcttctgtc
    26521 ggcatttcct tgcctgtcca cagttgaaaa gcttctgcag cttgatagag taacattccc
    26581 agaccattga ctgctggatt gccctgactt ctagcccatt tcaaaaacgg tgtttcaaag
    26641 ggttggtata tgatatctgc aactaaaaga gtttctggta agactatgtt ttcaggaaca
    26701 ggagaggatt ggccatccat gcccacactg gtggcattaa ctagcaaatc cgactcggca
    26761 atccttgctt gcagttcaga aacatattct aaagcacaca aatccacttt aaagcctgtc
    26821 tgctcctgta acttgtctag gtaaggtctt gttttttcca tagaaacgga acgaacaaag
    26881 acagaaatct gactgacgcc atccaaaata gcctgtgcca agattgattt agccgcacca
    26941 cctgcaccca gcagggtcat ctttttacct gaaattgtaa aagaaggcaa gcacttaaaa
    27001 aatcccttgc catctgtatt atatccaatt aaattgccat tctcattgac aaccgtatta
    27061 accgcaccaa tcaagcgcgc ttcatcgctc agctcatcca aataaggaat cacctgctcc
    27121 ttatagggca tggacagatt gatgccaaac atctggtagc gacgaatatt ggccactgtt
    27181 tctaccaagt cactcgcttc aatctcccaa gccacataag caccgttggt agctgtcgcc
    27241 tcaaaggctc tattgtggat gaagggagaa atagaatgct taataggatt ggcaacaact
    27301 gcagctaaac gtgtatagcc atcaagcttc atccaaaatc tccctgattt ttttcatgct
    27361 agctagagaa atctgcccag gggcactaac ctcatccaga ctggcaaaag accaactcga
    27421 accagtcaca tccgcagtga tacgagagac cttgcccacc ttacccatag aaatggtcac
    27481 atattcctgt tcaggattga gggttttaaa gcctcgtgta tagttcatca agtctaagac
    27541 atcctgctcc gtgtgagcca tcaccgcaac cttaacaagt tttggattta gaatcgtcaa
    27601 ctctgacaag atttccatca tgttctcagg tgtttcttgg aaattatggt aactcaaaac
    27661 aagatttggg aagtccagca tttcctcaaa aacatccttg tagctatagt actcaaaatc
    27721 aatatagtct ggttgataga gttgcgcaac ttccttgatt agatggatat actcttctgg
    27781 agaaaggtcg atttctccac cttcggagcg agttcgtagc gtgaaaacca actcacggcc
    27841 tgcgaatttt tcaaaaatgg ctggagctac ctgcaaaatc gcttctttag gcagatagtc
    27901 ggcacgccat tcaatgatgt cggcatccag gtacctcgtg gcatccagag cctgagcctc
    27961 ctctaaactt cttggcatta ctgaaacgat taatttcatt tactaacctt catactaatc
    28021 accttgaggt aattactact ttcatctttt ttattatagg caaaatctgc tggaagacca
    28081 tatttgttta aaatctggta acttcttcct gcaaaacctt tatcaatttg ttctgtaaat
    28141 ttctgacggg aaacattggc agcattggta ctggcaatga taatccctcc cggatttaaa
    28201 atctcaagac tctgggaaat caacttgtga taatccttgg ccacagagaa agtttgtttt
    28261 ttattccgag caaagctagg cggatctagg acaatcacat cgtaggtcaa gtctttgcgt
    28321 ttggcatatt tgaaatactc aaagacatcc atgactataa aacgatgctc gtctgtgctg
    28381 agcccatttg cctgaaaatg cgcttgagac aattttcgtg aacgtttggc tagatcaaca
    28441 gaagttgtat ggctagctcc tcccatggcc gcagctactg aaaaagccgc tgtgtaggaa
    28501 aacatattga gtaaggattt acccatagcc aagccgtcaa ctaaactacc gcgaacctca
    28561 tgctggtcta ggaaaattcc tgtcatcaag ccatcattca taaagacttg atacaggaca
    28621 ccattttcta aaacagtgaa aaagtcaggt gcttcttgac cataaacatg ggcagattca
    28681 tagtccaaac ccttaaagcg gattttctca taagctccta aaacctcagg gaaaacctgt
    28741 ctaaaggctt ctgatatagt ctgacgaatc tgataaacat aagagttata ccaagaaaag
    28801 acggcgtagt cgccataaag atccactgtc agacccccaa agccatctcc ctcttgatta
    28861 aagagacgat aggcagttgt taaatcgtct tgatagtagg cgcttctctt ttctttggct
    28921 tttctaaaca acgtttcaaa gaaagcttga ttgaaggcca ccttgtcttt gctgacaaac
    28981 cagcccaagc ccttgttttg ctgagaaagg taggcagtcc caagaaagtt tccttcctga
    29041 ccctgcacct ctacttcctg atccttaaga ttgacattct caagatcact ggcttctagt
    29101 aaaactagcc ccttagcaag cttcttttca acccttttgc tgactcttat tctattcata
    29161 actaccatta tatcaaactt ttagacaatt ctcaaaaaag aaactaccct tgctttttta
    29221 ctcttctttt aaaaaatggt atactagact tcctgcaaaa ctagaaagta aatgtgtaag
    29281 aatcacaata aaaaatgctc ttccgtcttg gaggagcatt ttttttatca acgaaaatca
    29341 aatagcaaac tatgaaacta gcctcaggtt aactgtgaga ttataggtag agaggttgta
    29401 tcagcaatat gtgtctgtca aatttagtga caaaggtagt agaagaaaga taaagaaata
    29461 aatcagcttc agtaggtatc tggaaaattt gattttatag agaagccttt tgttacaaac
    29521 tcaatatact atcaataaat aatattatag aagcaacaat aattataatt tcacctatct
    29581 gcatcattct atttcgaact ctaaatatat gttctatcaa aaatacttga aaaagacaca
    29641 ttataggaat taacgttttt gaaattgaaa aatatccaaa taaataaact ataaacaaca
    29701 aaaatagaac tatattatat ttcttattca aaacattcct ccctatattt ttgattacca
    29761 atcttaatca tttacaacta cattctaaca aactataaaa gcgtttgtcg aattgaattt
    29821 atcaagcaag caaccaacta tctcatcttt tttctatttc tgccaatatg cgtgacaggt
    29881 aataatgata gccaaaaata gcaagagcaa gcaagacgat aagagctcct actcccaagt
    29941 tgatggcaag gataggggag agagactgaa ccaagaatat gctcccaatt acaagggcca
    30001 tcaggattgc actataaata aacaataaaa ctatggcgac tatgccattt gaacgattca
    30061 ccaggtccgt aatgctactc caattggttg acagattttt aacgtcctta aagtaatggt
    30121 ggcaagaaag gatgacactg gcaatgatcc agactacaag aaggtaaatc atcgaaagga
    30181 tggacaagcc tagatataga gaaagaccga gcaaagtcag aattggtaaa aaggactgaa
    30241 cagcaaagat aatccaaaat ttcactttca cataacgagc aaagtcaaag ggtaaactct
    30301 tcagaaaatc aacattttcc ctctccaagg acaaggcaat tgaatgcagg ctggtgatat
    30361 tgttattgac aactgctata aacactccta caaaaaacaa gggtaaccag tatggaggat
    30421 gaatgtctgg aactatctga gaatctcgga ttttggaaat cagactgatc atcatgagat
    30481 aaggaaggaa agcacttgta aaaagcactg taatcacgcc agtcccctgt cccaagaggg
    30541 tgaggtggta gcgtaaaacc atacggaaaa agcccttttt agtagttgaa atcctctcct
    30601 tgctgcgacg ttcttttttg accttctcct cactgttaag caggatcacg tcataaaaac
    30661 gaggaaggac cttctttttg gtcaggtaaa gcaagaaaac agttaaagct atccaagcga
    30721 gcagacccaa tatggcttct attgaaaaag gctccactgc tattttgtaa aagatatgaa
    30781 gaggataaag gagaaatgga atgtctctaa ctttgtcaac aatacttcca aaagtcgact
    30841 gtagaaagaa gacaaatatt aaaggtatga gaactcctat cccaatcatc acattcgaaa
    30901 aaatagactg atactttctg aagaccgtag tttgagccaa gaaatgcact gccactacca
    30961 tcactagagc cacagagaca aataataagg tcaaggacag tagcatcaaa ggcaaaccca
    31021 gccatagaga aggagctagc ctaatgtaga ggactagaaa ataagctagg attggtacaa
    31081 ttccagttag agctggcaaa aggacagaca gtcctttagc aattataatc tctgattctt
    31141 taaaggcata gggcctatac gataccaaat ccttactctc ataaaagaca ttgtaaaagg
    31201 ccgttaaaga agttgaaaag gcaatcacta gtaaaatagc aaccatcaaa ctaaaataaa
    31261 taggtatttc ctcaaaagga aaatgaatgg ctatattact aaaacagatg agcatcaaga
    31321 gactggaaaa aatgtaagaa cttaggactc tagcggaaac atttactttt ttcccaggat
    31381 tcttagcctg cttctttcgt agattagcca gattagcttc ttgagatgaa tagaggatat
    31441 tgatatcaac taatttttta atgaccttga gacgcatccg caacctcctc ttttctacca
    31501 gcaagactaa ggtagatact ttccaaagac tggtctgggt ggtctttcct caagtcctct
    31561 accttaccac aataaatcaa atgccccttt ttcaaaatag caatccgatc acagacttgc
    31621 tcagctacct ctagcacatg agttgaaaac aagaccgtct tccctttttg tgcatgttcc
    31681 ttcatcatct gtttcaaatc aaaggcagcc tggggatcca aaccagtcaa gggttcgtcc
    31741 aagacccaaa tatcaggatc agacaagagt gccccgatga caaaaacttt ctgacgcatt
    31801 ccatgagaaa gggtttcaat aacctgataa cgattttcag caaaatcaaa aacgttcaat
    31861 agcctagcta gactagtctc caagtcagag ctagtcagat cataggatga ggcgatcaat
    31921 tcccaaaatt cattggccgt taaacgtaaa aataagtcag gcgagtctgc tacgtagcca
    31981 atctttcgtt tgatcgccaa gcgattttcc gataactcct gaccatctac caaaatacga
    32041 ccactgctgg gtgaaatgat actgactagg gattttatgg tggtcgattt tccagcccca
    32101 ttatgaccaa tcaagcccat aatctctcca ttttcaatct gcaaattgag attactcaag
    32161 gcctctttat caccatacaa cttactaaca ttttgaaatt caatcatggg atgactccta
    32221 tctttatctg tattatacat cgctatacta gcactttgat acaaaaaaat caatacttta
    32281 ttttaagtag ttaaaatagt ttccttctat cttacgcaaa cgtttgctcc gagagatact
    32341 ttatgataga ataaagaaca agattgacaa gtaagaggaa acatcatgca aaatcaaaca
    32401 ctcatgcaat actttgaatg gtatctgccc cacgacggtc aacactggac gcgtctggct
    32461 gaaaatgctc cacacctagc tcatctgggg atcagtcacg tctggatgcc accagccttc
    32521 aaggcaacca acgaaaaaga tgtcggctat ggggtctatg acttatttga cttaggagag
    32581 ttcaaccaaa aagggactgt ccgcaccaag tatggtttca aagaagacta tcttcaagcc
    32641 attcaagccc ttaaagcgca gggaattcaa cctatggccg atgtagttct caaccacaag
    32701 gctgctgctg atcacaggga agcctttcag gttattgaag tggatcctgt agaccgtaca
    32761 gttgaacttg gagaaccctt caccatcaat ggctggacta gttttacctt cgatggtcgc
    32821 caagatacct acaatgactt ccactggcac tggtaccact tcacaggtac agactatgat
    32881 gccaaacgcc gtaaatccgg gatttaccta atccaaggag acaacaaggg ctgggctaac
    32941 gaggaattgg tcgataacga aaacggaaac tacgactacc tcatgtatgc cgacctagac
    33001 tttaaacatc ctaaagtcat ccaaaacatc tatgactggg ctgattggtt catggaaacg
    33061 actggtgtag ctggtttccg tttggatgcc gttaagcata ttgactcttt ctttatgcgc
    33121 aatttcatcc gcgatatgaa ggaaaaatac ggtgacgatt tctatgtttt tggtgaattt
    33181 tggaacccag acaaggaagc caatctggac tatctcgaaa aaacggaaga acactttgac
    33241 cttgtcgatg ttcgtctcca ccagaatctc tttgaagcca gtcaagctgg cgcaaactat
    33301 gaccttcgtg gcattttcac agatagcctg gttgaactca agcctgacaa ggctgtgact
    33361 tttgtcgaca accacgatac ccaacgagga caagcccttg agtctaccgt tgaagaatgg
    33421 ttcaagccag cagcctatgc cctcattttg ttacgccaag acggccttcc atgtgtcttt
    33481 tacggagact actatgggat ttcaggccag tatgctcaag aagatttcaa agaaatcctt
    33541 gaccgcctcc tagccatccg aaaagacttg gcctatggag aacaaaatga ctactttgac
    33601 catgctaact gtatcggttg ggtacgttca ggtgctgaaa atcaatcccc aatcgcagtc
    33661 cttatctcaa atgaccaaga aaacagcaag tcaatgtttg tcggtcaaga atggactaat
    33721 caaacctttg tagatttact tggtaaccac caaggtcaag ttacaattga tgaggaaggt
    33781 tatggacaat tccctgtctc agctagatcc gtaagtgtct gggcagtcaa taccatctaa
    33841 tagctcataa taaccaagct aggtccaagc ggatttggct tttttgtatt cacaaaaaga
    33901 cctacccaaa tggatagatc tttacttgat tacaatttac ctgctactgc atccaacaat
    33961 tcttggatct taggttggtt gcttcctcct gccatggcca tgtctggttt accaccacca
    34021 cgtccatcga tgattggtgc taattctttg acaaggtttc ctgcatgaag gtcttttgtc
    34081 ttgcttgcta caaggacatt gactttgtca ccgatagcgg caactaggac aagaagatca
    34141 gagtagtctt tttgtttcca gttatctgca aaggtacgaa gggcacctgc atcggataca
    34201 gacacttgac tagcaatgta acgatgaccg ttgacttcct taacatcttt gaagatatcg
    34261 cctgcggctg cagctgcggc tttttctttc aactcagtat tttctttttg aagttgacga
    34321 agttgttctt gaagtccttc taccttgtga ggtacttcct tgacttgagg tgctttcaag
    34381 gttgctgcga cagctttaag agcatcctct tgttcacgat aggcttcaaa ggcttcctta
    34441 ccagtcactg ccaagatacg gcgagttcct gaaccgattc cttcttcttt gacaattttg
    34501 aagagaccaa tctcagaagt gttgccaaca tgagtaccac cacaaagttc aatagagtag
    34561 tcaccaatag tcacgacacg aacttccttg ccgtatttct caccaaagag ggccatcgct
    34621 cccatttctt tagcagtgtc aatatccgtt tcaactgtct taacttcaag agcttcccag
    34681 attttttcgt tgacttgctg ttcaatcgca cgcaattctt cagcagttac agcttggaag
    34741 tgggtaaagt caaagcgaag gaattcaact tcgttaagag atcctgcctg tgttgcgtgg
    34801 tttccaagga tattgtgaag ggcagcgtga agcaaatgag tcgcagtgtg gtttttcatg
    34861 acacggtgac ggcgattgct atcaattgcc aaggtatatt cttggttcaa ggcaagcggt
    34921 gcaaggactt caactgtatg aagggcttga ccatttgggg ctttctgaac attggtcaca
    34981 gtagccacaa ccttacctga ctcatccaag atttgtccgt ggtcagcgac ctgtccaccc
    35041 atttcagcat agaatggcgt ttccgcaaag ataagtgagg tagttccttc tgatacagct
    35101 tctacttctg cattgtcagc aacgatagcc accaatttag aagacaattg gctagcattg
    35161 tggttgaaga cactttctac agtgatgttt tgaagagttt cattttgcat acccattgag
    35221 ccacccttga cagctgacgc acgcgcgcgt tcttgttgtt ctttcatggc tgcttcaaaa
    35281 ccttcacggt ctacagtcat accagcctct tcagcaattt cttcagtcaa ttcaactggg
    35341 aatccgtatg tatcatagag tttaaagaca tcttgaccag caatgacaga ttgacctttt
    35401 tctttcaagt ctgccacaat gccttgggca aagtgttgac ctgagtgaag ggtacgggca
    35461 aatgattctt cttcgctctt aacgattttc tcaataaagt cacgtttctc aagcacttct
    35521 gggtagtagc tttccatgat ttttccaaca gttggaacga gtttgtaaag gaaaggctcg
    35581 ttgataccca atttttgacc atgcatagaa gcacgacgga gaagacgacg aagaacataa
    35641 ccacgacctt catttcctgg aagggcacca tcaccgatag caaatgaaag agaacgaatg
    35701 tggtctgcga taaccttgaa gctcatgttg tcgccatctt ggtcataaac cttaccagac
    35761 aatttctcga cttcacggat aatcagcatg aagaggtccg tttcaaagtt ggtcttagcc
    35821 ccttggataa cggccaccaa acgctccaaa ccagcgcccg tatcaatgtt cttatgtggc
    35881 aattccttgt attcgctacg aggaacagca gggtctgcgt taaattgtga caaaacgatg
    35941 ttccagattt cgatgtaacg gtcgttttca atatcttctg caagcaggcg aagaccgata
    36001 ttttctgggt caaaggcttc cccacggtca aagaaaatct ctgtatctgg tccagaaggt
    36061 cccgcaccaa tttcccagaa gttgtcctca attggaatca agtgacttgg atccactccc
    36121 acttcaatcc agcggttgta agaatcttta tcatctggat agtaagtcat gtaaagtttc
    36181 tcagctggga agtcaaacca ttcagggctt gtcaaaagct cataagccca agtgatggct
    36241 tcatcacgga agtaatcccc gatagagaag ttccccaaca tttcaaacat ggtatggtga
    36301 cgcgcagtct tccctacgtt ttcgatgtcg ttggtacgga tagccttttg ggcattggta
    36361 atacgtggat tttcagggat aatggtcccg tcaaagtatt tcttgagggt tgctacccca
    36421 gagttgatcc acaaaagagt tgggtcattt acaggaacca aacttactga tggttctact
    36481 gagtgacctt tggtcgccca gaaatcaagc cacatttggc gtacttgtgc actagatagt
    36541 tgtttcatat tgtctcctta tttacttgtt taatgtgatt ggctttccag catttccaca
    36601 tagtcaatcg cgacacagag ggaaatgact aggtctgcat aagcgtcttc aagaaccgtt
    36661 acggtatagg tagaggtcag atggaagagt tccttcttaa tttccgcaat caactgatcg
    36721 cgatcatcca gcaatttgaa attcaaatcc cagatattgc cctcgatacg aagacctaga
    36781 ttatcaaact catacttatc tcgccaaaag gtcaacttct tacgaatgac aaaactcgag
    36841 ccatcccgaa gctgaatctc aaaacgagga agcaaggtca agatttcttt actgatctga
    36901 ctgacttgtt caccagccgc atcatagatg gtaaaagttt tgggaatctt aaaaaatgat
    36961 ccctccacct gataggcaat ttctcccctg tcatccttga tagcgaagcg ttcgcctcca
    37021 agacgaaact tttgtttgac aagaaatgtt ttcatcaaca cctccaaaaa tcaaaagaca
    37081 agctcatatc acgaagggcg aaaaaccgcg gtaccacctt cattcaatga acttgtcatt
    37141 ctcttgttct tatgcaattg tatgattgag tagcatgact ttctagctta gatggctcgc
    37201 agcaccgcca tttctctgga ctaagacaag tgaaaattaa ttctcaactt tcttattata
    37261 acgttttttt aagcttgcgt caactggaaa tgatctccgt tgaattagac caattcccta
    37321 catctctgat tactttttca ggatatattt tttcttactg ccgtttttct ttttatccca
    37381 aattttcata ttactaaaca cagctactag aatatttcca aatataaagg tgcctatcac
    37441 ccaatatatg gactcagttg ttaggtattg tcgatccaag ccatccttta aatggaatag
    37501 tataacagtt tggttaacaa tcataaaggt tggccagaaa ctttttttga aaaaagtaga
    37561 cattttcatt atttgttgcc gctttctgta aggttaatac tcaataaaaa tcaaaaagca
    37621 aactaggaag ctagcctcaa gctgtacttg agtacggcaa ggcaacgctg acatggtttg
    37681 aagagtatag gcttagtata ctactaggca agcaaataag caaataaaca actagaatag
    37741 aaaaagatag ggctctaaaa actgacttct attccttaaa aacgaaccag cttgactgat
    37801 tcgtcttctt acgtttatct cctacttccg atacatttta aactgtagga agaggtcgct
    37861 atatttccct gtccatttat ggtcaaattt ctcataaact tctaggtgtt tcatggtttc
    37921 aacatcggga tagaaggcct tatcttcctt tgtttcctct gggagcaatt ccttcgctgg
    37981 taggtttggt gttgaatagc cgacatactc cgcattttgg agagcatttt caggtttcaa
    38041 cataaagttg ataaaggcat aggctgagtt ttggttttta actgttttgg gaatgaccat
    38101 attgtcaaac caaagattgc tggcctctgt cggtaccaca taacgtagat tttcattttt
    38161 ttctaacatt tggctggctt caccagagaa ggtcacgccg attgcaacat tattctgaat
    38221 catatagccc ttcatctcgt ccgcaacgat agccttgata tttggagtca gtttgtagag
    38281 cttatccact gtctcttcca actgctgcag atccttggag ttgaggctgt agccgaggga
    38341 attgagtcct agtcccagca cctcacgcgc cccatcaaag agcatgatag aattcttata
    38401 ctccggcttc caaaggtcat cccaatgctc aggcgcttca tctaccatgg tttcgttgta
    38461 gacaattcct aaggttcccc agaagtaagg gatggagaat ttattacctg ggtcaaagga
    38521 ctggttgaga aactctggtc cgatattttc gattccttca atttttgaat aatcaagcgg
    38581 aaccaagagg tcttcgtcct tcatcttgtt aatcatgtat tcacttggaa tggcaatatc
    38641 gtaggtcgtt ccaccctgct ttatcttagt gtacatggct tcgttggagt caaaagtctc
    38701 gtactgaact tgaattcctg tttcttctgt aaactgagtc aagagttcag gatcgatata
    38761 gtctccccag ttatagataa ccaatttttg actatctcga ctattgattt tactatctaa
    38821 atgagtcgca attccccaca agacaaggat aatcgctgca attcctgcta aaaatgaata
    38881 gatttttttc atgcttgctc ctccttctca cgagagataa agtaataacc tacaactagg
    38941 ataatactaa agagaaagac tagagcagac agggcattga tttctaagga aatccccttg
    39001 cgagcacgag agtaaatctc gactgatagg gttgaaaagc catttcctgt tacaaagaag
    39061 gtcacggcaa agtcatctaa cgaataggtg aaggccatga aataaccagt aatgatagac
    39121 ggagtcaggt aaggaagcat gatttccttg aacatctgaa attgactagc tcccaagtca
    39181 taggccgcat gaatcatgtc gccattcatt tccttgagtc gaggcaagac catcaagacc
    39241 acgataggaa tggagaaggc cacgtgacta gatagaacgg tcaaaaagcc aagtgacaac
    39301 ttgagttggg taaagagaat caagaagcta gcaccaatca taacgtcagg cgcaaccatg
    39361 aggatattat tgagtgatag aaaggcttct tggtatttct tacgagactg gtagatgtaa
    39421 atggcaccaa aagtcccgat aatggtcgct atcaaggctg ataggaaggc caagaaaaat
    39481 gtctgagcca aaatcagcat gagtctccca tctccaaaca tggtttcaaa gtgagtccag
    39541 ctaaaacctg taaagctatt catatcatca ccagcattaa aggcatagcc aatcaagtaa
    39601 aagataggca ggtagagaac cagaaagacc agtcccagat aaaggttggc aaattttttc
    39661 atcgttctct cctttcctta gtcacccaca tggtgatgaa catggtcagg atgagaatca
    39721 caccgatggt tgaacccata ccatagttgt cattggttag aaaattctgc tcaatagccg
    39781 tccccaaggt gataacgcgg ttcccaccaa tcaaacgggt cagcatgaag agactcaaac
    39841 ttgggataaa gaccgactga accccacttc gcacaccgtt catagataga gggaagatga
    39901 catgacggaa ggtctcccac ttagttgcac caaggtcgta actagcattg atgagattat
    39961 tatccatatc gtctaagaca ttgaaaatcg gcaaaatcat aaaggggagc tcgatgtagc
    40021 ttgcgacaaa gataaaggaa aaatcggtaa aaagcaactg ttgtgaacca attccgataa
    40081 attccaagaa ttggttaata gagccatttt gaccaaaaat cccgataaaa gcataggctt
    40141 taaggagcaa attgatccag gtaggaagga taatcagcat gagccagagt tgacggtgct
    40201 tgagacgggt caaaaagagg gctgttggat agctgataag caaggtcaca aaggtcacaa
    40261 tccctgcata aagcactgag ttgaaactca ttttgaggta ggtcaagttt tgtgacgcaa
    40321 agtaagattt ataattttct aaactaaact ggccttcaat gttgaaaaag gattgtccga
    40381 aaatcaagac caagggtgcc aatacaaaga gggcaatcca aagcatgtag ggcactacaa
    40441 agagtttaga gcttgttttc ttcatctctt tcctcctcga tcgcattgat cagacctgct
    40501 tcttgctctt cgatttccac gtattcttcg atacgagcat cgaactcttc ttcggtttca
    40561 ttgagacgca tgatgtggat gtcttctgat tcaaagtcca gaccgatttc ctcacccacg
    40621 atggccttac gggttgagtg gatcatccat tcatttccaa gttcgtcata ggcgataatt
    40681 tcataatgaa ctccacggaa gagctgggtg tcgaccttaa cttggagttt gccttcttca
    40741 ggaagggtaa tgcgcaagtc ctctggacga ataacgacct caacaggttc atttggcttc
    40801 atcccaccat caaccgcttc aaagcgtttg ccgttaaatt cgaccaagta gtcctcaatc
    40861 atggtacctg gcaagatgtt tgactccccg ataaaggtgg caacaaagtg gttgattggc
    40921 tcatcgtaga tgtccacagg ggttccagac tggacaatct cgccatcatt cataacgaaa
    40981 atccagtcac tcatggcaag agcttcttcc tgatcgtgag tgacaaagac aaaggtaatg
    41041 cccaatcgtt gttgtaattc acgcaattcg tactgcatgt ctgttctcaa tttcaagtcc
    41101 agcgctgata aaggctcgtc caacaagacc acacggggtt ggttgatgat agcacgggcg
    41161 atggccacac gctgacgttg tcctccagaa agtttgcgga tggaacgttt ttcataacct
    41221 tccaactgaa ccatcttgag aacttccgct acacgctgct cgatttcttt cttatcaatt
    41281 ttacgcaagc gaagtggaaa ggcaacattt tcaaacacat tcatatgtgg gaacaaggca
    41341 taggattgga agacggtatg tacgtcgcgc ttgttggttg gaatatcatt gatacgaaca
    41401 ccgtctagca tgatatctcc tgtcgtcgca tccagtaaac ctgcaataat gtttaggata
    41461 gttgatttcc ccgaaccaga tgcacctaga agggtgtaga atttcccttc ttccaactca
    41521 aagttgatgt ctttgagaac cttggtgttg ctgtcttcaa aaactttaga gacgtttttg
    41581 aattcgataa ttggtttttt caattggtat aaattccttc tttttcatag attaacagat
    41641 cggggctctg tcaggtcgcc actacctctt gcagggagta aaaccacctg catacatctt
    41701 cgctaccgat aggctttcac cccctttcca tgacatagca gcagcttttc aaatacagct
    41761 tcctacttgc tttcacccaa gatccggact tctctttcaa gcgtaatacc tgagtgttcc
    41821 ttgacttttt cgataaccga ttggatcaag tcctcgtagt ctttggccgt tccatctgcg
    41881 acattgatca taaatcctgc atgcttttct gacacttcta cgccaccgat acgatagcct
    41941 ttcaagccag cttctgaaat taactgacct gcaaaatgcc cgactggacg cttaaagacc
    42001 gagccacaag atgggtattc caaaggttgc ttgagttcac gtaggtgcgt caagcggtcc
    42061 atttcctgct tgataacctg atgggttcct ggagctaggg caaatttaac tgacaagaca
    42121 actgcaccag actcctgaat agctgaatgg cggtaaccaa aagccaagtc tttagcagac
    42181 agggtttcga tttctccatc cttggtcaag accttacaag actgcaagat gtgagcaatc
    42241 tcgccaccat aggcacccgc attcataaag acagcaccgc caacgcttcc tggaatacca
    42301 caagcaaact caaagccagt caaactatga cggagggcaa tgcgagttgt ttcaatcaag
    42361 ttagccccag cttctgcttc aatggtatag ccatcaacag aaacgttatt gagcttgtca
    42421 cacaagatga caaatccacg aatcccacca tcacgaacga tgatattgct tgcattgcca
    42481 agaaccatcc aagggatatt ttcttggttg gcaaatttca caacgcgagc caactcaaaa
    42541 cgatttcgtg gaaagaccaa ataatcagcc tctccaccta cttttgtata actatagcta
    42601 tgcaagggtt cctgaaaacg gatatcaatt ccttctaaga tttcaagcat tttttctctt
    42661 acagacatgt cactcttcct tttacaaaat tcattccatt ataccatttt tagagacatt
    42721 tgacgaccat aaaaatacct tgtttggatt ttgcataaga aaaagaggtt cccctctttt
    42781 tatgattttt tacaaaagat ttccttggtt ccataggcga ccagaacgag ctccagtgct
    42841 agaatcactt caaccaagac tggatttgtc aaccagccta cttggaaaag agatggtgcc
    42901 agatcaaaga aggcatgcaa gccataggct gttaggagat aaatccattt cttctggcga
    42961 acagcttgat aaacccaaac tgtcaaaagt aattggaaac caagcgccaa gattcgctca
    43021 aaaccaagca aataaatctg ccagaccgaa agtgactgaa tggtttttaa catattttca
    43081 gacagtaatt gcataacctg tggattctga gtttgaactg ccgaaagaac aatgtaaaga
    43141 ttgagtaaac tagtaaggcc tagaaaaatc aactccaagc caccatgccc caatccatag
    43201 gccaaggcgt ctgccttttc caaacttctc tttttctcca accatttgaa gaaaacaaga
    43261 cgagccgttt cctcaaaaaa tgctaccatg gctaggccat agataatata gataagcgga
    43321 tgaacttaca agagggcaat actaccgtct ttttgaggat gcaaaatcaa gatatgcacc
    43381 agtttttcca aaatctgcga agagacaaag aaagcgactg cacccaagtc caagacagct
    43441 agattaatct ggtatttctt tttggcaaac caaatgcttc ctatcagaaa agctagcaac
    43501 agcaacatgg taataatgat atgaatggtc attttcttct cctattctgc cttttcaata
    43561 tcttttttca tctcgtcaac attgaattta gcaaacaagt attgacggtc ttggactggg
    43621 aaacgttggt ccaactggtc aactgctccc acctcgataa tgccttcctt gatgacaaaa
    43681 tccatgacat gtccaccgaa ggtcaaatca tctgatatga agtgcagatg gtagcctgcc
    43741 acactgaccc catggaaaat ctcaggcgtc cagaaaccaa caatagtccc cgcaacattg
    43801 tcacgactat attccggttg atgggttgcg acatcagcaa acttggtatc gggtgttgac
    43861 ttaggaatca tacgcacatg catatgtgaa aattcccccc gaatcttgat agagcggaaa
    43921 agattttccc catcataata agactcaatt cgttctttca attccttgtc tgtcatctca
    43981 aagcgctggc ggaaaatgac ctctgcctga tgcggtacca ctgcagcgta aggaataagg
    44041 gcatctggtg acacttccac aatttctggc tggtctcctg accctttggc ctgataagcc
    44101 ttgccatcca agacaatcaa ttccccatca atggaatcca aggttcccaa accaaggtca
    44161 ccatgttcta gcaattctcc tactgtcata gtaccaccat aaaggccagc catcaaggct
    44221 ccaagagtat tgtattgaaa taatttcact ggttcctgca cgttcttatc cattcacttt
    44281 cttgtctgtt attctatgaa tcttactcct aagtatacca catttgcccc tagatgtgaa
    44341 cgagagaaac gctctagaca ttgccaagaa ggaaaaaaaa gggtacaatg taacaaaatc
    44401 aagggaggtc tggaatgaag aaacaaagca agtacaaaga ggtcgtttcc tatctgaaaa
    44461 atggtatcga gtctggacga tttccgacgg gtagtcgcct gccttctatc cgtcaactga
    44521 gccttgactt tcactgcagc aaggacacca ttcaacgagc cctgctggaa ttacggcacg
    44581 aacaatacct ctatgccaag ccccagagcg gctactatgt cctagaacaa gggcaacatc
    44641 aagacctaga aatcgaggtt accgacgaac atgccagtgc ctatgacgat ttccgactct
    44701 gtgtcaatga aaccttgatt ggccgagaaa actacctctt caactactat gacaatcaag
    44761 aaggattaga agacctaaga cagtccattc acaaactcct ctttgagcaa gctctctact
    44821 gcaaggctaa ccaactagta ctgacttctg gaacccaaca agccttgttt atcctctctc
    44881 aaatatcctt tcctagacaa gccaaggaaa tcttggtgga acagccaacc taccatcgga
    44941 tgaatcgcct cttgattgca caggggctgg actatcaaac gattgaacga ggcattgatg
    45001 ggattgactt ggaggagctg gaaggccact tcaaaacagg aaaaattaaa tttttctaca
    45061 ccattcctag atttcactat cctctgggac attcctattc tgagcaagac aaacgatcta
    45121 ttcttaactt agctgccaag tatgatgtct atatcgtaga ggacgattat ctgggtgatt
    45181 tggactccaa gaagggccaa accttccact atcttgatac agaggagcat gtcatttata
    45241 tcaagtcctt ctcgaccagc ctttttcctg cccttcgtat tacagcactc attcttccaa
    45301 atgctatcaa agaagcattt gtggcctaca aaaatatcct agactacgac agcaacctca
    45361 ttatgcaaaa ggccctgtca ctctatattg acagtcaatt gtttgaaaaa aatcgtttgg
    45421 ctcgcttgac caatcatgaa tcttaccaaa aacaaatcga ggaaaggata actaaaacac
    45481 cttgtcccct tcctcattat cccctacacg atggtttatt gctagacctg agacagtatc
    45541 ctaaaatcgc cagtctcaaa cacagtcaac tgggcttgga cttctttgaa gaggcctatt
    45601 taagcacctg tccttatcaa tttgccaagg tgtccctaga caatctggaa aatgttttaa
    45661 actatttaaa agcagaattg gaatgacatc caactctgct ttttcttatt cttcaacttc
    45721 agttagactt gctgcttttt caagatagct ttggtaagtt ccttcatcct taagtttttg
    45781 gatgaccttg tctaccactt ctttcaaatc atcactattt tttctaagag caacggcatt
    45841 ggcgtcgccg tccttcatct tcaagctgac agttgcgaca gctaagccag cgtttttagc
    45901 agcataacta agtgcaacag gctcatccat atgaacagca tctatttttc cagcctgcaa
    45961 ttcattgact gcttcaccca tattagttag ggaagttaat tgagcttttg gcaattgttc
    46021 cttgaccatt gattctggaa cagtcccttt ttgggctgca atattagcac tttctaggct
    46081 agttaaatcc ttgtattttt ccacatcagc cttacgaacc aagaaactaa tcttgttttc
    46141 atagtatggg attgaaaaat caaagacttc ttttctctcg tcagtagcac taattcctgc
    46201 aactgctagg tcagccttac cagtttgaag actggtcaaa acattgtcaa aactcatgct
    46261 tgagatttcc aacttaaccc caagttcatc agcgatagcc tgagccatgt cgatgtctgc
    46321 accgactacc tggttctttc catcaaccaa tgattgaaat tcaaagggtg catagtcagg
    46381 actggttgcc acaactaatt tccccttttg cttaatggcc tcaacagcag actgagaacc
    46441 attagaacct gactgacaag ctactaagaa gaaacttgca agaaaattac ataaaacaaa
    46501 tatccatttt tttgatttca taaataaaca acctttctgt taattttgta taattataac
    46561 gatatccaag ttacttgtca agtgtttttt aaatttttat ctcaaaaata ttttttcgtt
    46621 caaaaaaagg agccatcagt tgatttcaag ctccttttta tacagaatta aactatttta
    46681 tagttcgaca atcttacctg tttcaaagta gacaacccat tcacagatat ttttagcata
    46741 gtcaccgata cgctccaagt aggaaataac ttggaaataa tcacgacccg taacaatggc
    46801 ttctggattt ttcttaatct cttcagtcgc aaggtcacgg atagtttcaa aatagtggtt
    46861 aatttgctca tccatggagg ccacccggta tgcgtcgtca acagaaccat taagataaag
    46921 atcaagtgct gcttcaacaa cgcttttaac ttcacgtccc atttttttaa tttcttcctc
    46981 tacagctggg atgcgttctt cccccttcat acggatggtt gcctgggcaa tggctacagc
    47041 gtgatccccc atacgctcca catctgatac agccttaagg acagtcaaga ctgtacgcaa
    47101 atcttgagag actggttgtt ggagtgcgat catttcaaat gatttctttt ccagtttcac
    47161 ttcgtattca tttacttctg catcatcttc gatgacctct tttgccaggt cacggtcatg
    47221 cgtgacaaaa gcacgtaccg tacgattgat ttgtgagagc acttcttgtc ccatagcgta
    47281 gaactggtta tgtaatttct ctaaatcttc ttcaaattga gatcgtaaca tctttcatct
    47341 ccttatccaa attttcctgt aatatagtct tccgtttcct tgtgttgggg atcaaggaac
    47401 atctgcttgg tatcattaaa ttcaatcaaa tctccatcta ggaaaaatcc tgtcttatca
    47461 gagatacgtg aagcttgctg catggaacgg gttaccagaa gcatggtgta cttgtctttt
    47521 agaccataca aggtttcctc aattttacca gctgaaatcg gatccaaagc cgaagttggc
    47581 tcatccaaga ggatgatttt aggactagtt gccaagacac gggccacgca gacacgctgc
    47641 tgttgaccac ctgacaatcc aatagctgaa tcatatagac gatccttgac ctcatcccag
    47701 atagaggcac cttgcaaggc tttttctacg gcttcatcca gaacctgctt atccttaatt
    47761 ccattgatac gaagcccgta gacaacattc tcatagatag tcatagggaa aggattaggt
    47821 tgttggaaaa ccattccgat ttccttacgt aattcaaccg tatctgtacg cggactgtag
    47881 atgttgtgac cattgtacac cacggatcca gttgtggtca cctctggatt gagatctccc
    47941 atgcggttga gagacttgag gagggttgac ttccctgatc cagatggacc aatcaaggct
    48001 gtaatttcct taggttggaa agatagggaa acactattca aagccttctt tttattataa
    48061 taaacggaca ggtctgatac ctgtaaaatc gcatctgtca tacggtttcc tttctaacca
    48121 aagtgaccag atacatagtc attggtggac tgtagcttgg cattttggaa aatagttgca
    48181 gtcttgtcat actcaatcaa atcacccaag taaaagaagc ctgtatagtc acttgcacga
    48241 gcagcctgct gcatattatg cgttacaatg atgatggtaa agtctttctt gagctcaaac
    48301 atggtctctt ctagttgcat ggtcgcaatc ggatccaagg ctgaggctgg ctcatccatt
    48361 aagaggatat ctggcttaac agagatggca cgagcgatac agagacgttg ttgctgacca
    48421 cctgataagg tcaaggctga cttgtggaga tcgtctttaa cctgatccca gagggcagcc
    48481 tgacgaaggg aggtttctac gatttcatct aggacttgct tatccttaac tccagcacgt
    48541 tcatgcgcaa aggtaatatt acggtaaatt gacttagcaa atggattggg gcgttgaaaa
    48601 accattccaa tgtgtttacg catttcataa acgttgattt ctggacggtt gacatcaatt
    48661 ccacgataga gaatctgccc agttacttta gcaatatcaa tagtatcatt catgcgattg
    48721 agactgcgta agtaggtaga tttccccgat cccgacggac caatcaaagc tgtaatttta
    48781 tttctttcaa attgcatatc aatcccctta atggattcat ttttaccata gtaaacatgg
    48841 acatccttag tagaaagggc tactttttct tcaggaaagg taaggatatg cttctcatcc
    48901 cagttatatg ttgacatggc ttctccttta ggcagcggtt aatttcttgt gtagatagct
    48961 tccgaactta cgagctccaa agttaaaaat caggataaag atcaggagca cagcggcaga
    49021 acctgctgat acaatggttc catctggaat agtgccttca ctattgactt tccagatatg
    49081 gacagccaag gtttctgctt gacggaagat agagatgggg ctagtcacac tgaggatatt
    49141 ccagttagac cagtcaagag ctggcgccga ttgccctgct gtatagatca gagctgcagc
    49201 ttcgccaaag atacgaccag atgccaagac gacacccgtt acaatacctg gaagcgcttc
    49261 tggaataaca acatgaacca ctgtctccca gcgagaaatc ccaagagcca gaccagcctc
    49321 acgttgggta tggtgaacgt gtttcaaact atcctctaca ttacgcgtca tctgaggcaa
    49381 gttaaagact gttaaggcca aggcacctga aatgattgaa aatccatact caaactggac
    49441 tacaaagatc aagtaaccaa agagacccac caccactgat ggtaaagagg acaagatttc
    49501 aatacaagtc cgcacaaagt tggtaacagg acctttttta gcatattcag ccaagtaaat
    49561 cccagctccc atagaaagag gtacagaaat aatcaaggta atgaccaata ggaaaaagga
    49621 attgtaaagc tgaatgccaa tcccaccacc tgcttgaaaa gcagaagacc ttccagtcaa
    49681 gaaagaccaa gagatatggg gcaagccccg aaccaagata tagagaatca aggaagccaa
    49741 gattgtcaca atgatgctag caatcgtata gaggacagct gttgcaagtt tatctaattt
    49801 cttagcgcgc ataatttttc tttcctcttt ctttcgtaat caatttaatc acactgttaa
    49861 aaactaagct catcaagagc agtaccaagg ccagtgacca gagaacatta ttatttacag
    49921 ttcccatgac agtgttccca attcccatag ttaatataga agttaaagtt gcagctggtg
    49981 tggtcaagga agttgggata acagctgagt tgccgacaac catctggata gctagagcct
    50041 caccaaaggc acgcgccatc ccaaagacca ctgcagtgaa aataccagaa cgggccgcct
    50101 tcaagatcac acgccagata gtctgccagc gagtggctcc catagcgaaa ctggcttcac
    50161 gataataacg aggaaccgca cgcaagctat ccgttgtcat aaaggttacg gtcggcaaaa
    50221 tcatgacaaa gaggacggaa atccctgaca aaatcccaaa accagtccca ccaaagacac
    50281 tgcgaacaaa aggaacgacg acttgcaagc caataaatcc gtacactact gaaggaatcc
    50341 caaccaggag ttcaatagct ggttgcaaaa tcttcgcccc ttttggtgat acttcggtca
    50401 taaaaactgc tgcaccaata gcaaagggtg ttgcgataag ggctgagaga atggtaacga
    50461 taaaggaacc caaaatcata ggaagggcac caaattcttt actagaaaga ttccaagttc
    50521 ctcccaaaag aaagtcaaag atattcacac cattgacaaa gaaggtcgac aagccttttt
    50581 gcgctacgaa aaccaaaatc atggccacaa ggatgactat caaagaaaga caggcaaagg
    50641 tcaaaccttt tcctaatttc tccagacgag aattctttga tggaagcaac attttcttag
    50701 ctaattcttc ttgattcatt attgtctccc ttccaacact gtcacagttc cggcagcatc
    50761 tttttcaacc ttcatttcct taatcggaat atacttcaat cctttgacaa tcccttcttg
    50821 ggtctcatcc gagagaacaa aattgagaaa ttctgcagcc aactcattgg gctgccccaa
    50881 tgtatacata tgctcataag accacaaggg ccaattattg ctacttatat tttctggact
    50941 taagtcatag ccattcaact tcatgctttt gaccgaatca tctatatagg taagagataa
    51001 ataagagata gctcctggac tttttgatac gattgatttt accgctccat ttgaatcctg
    51061 ctcctgactt tgcatggcag actgaccttc cataatgaca gtatcaaagg tagcacgaga
    51121 gccagagccg gctgcccgat tgataacaga gatgggtaag tccttaccac caacctcttt
    51181 ccaattggtt acctcaccta tgaagatttg acgaagttgc tctgtcgtta ggttatcaac
    51241 atcaacctcc ttattgacaa tcagagccaa gccagctacc gcgaccttgt ggtcaacaag
    51301 agcagaagca tcaattccgt ctttttcctc agcaaataca tctgaatttc ctatatcaac
    51361 tgccccagac tgaacctggg acaagcctgt accagaacct cccccttgga cattgaccgt
    51421 ttttccaaca tggatcgtgc caaattcatc tgccgctact tcaaccaagg gttgcaaggc
    51481 agttgagcca acagccgtta tggattctcc acgatcaatc cagctagcac agcctactaa
    51541 acaagccgtc agccaaaaag cgataagaga cagagcaagc ttttttcttt ttttcactgt
    51601 ttttctcctc gaaaataatt atgaatactg tgaatttttt aagtagttct ttatgagttg
    51661 acgcatgaat tcttaccaaa tttctgcgca attgattatt tatataatat aggctatatt
    51721 actctttcct aacctccttt tttcatatgt ggataaaatc tcttgtctat cccttccccc
    51781 attgtcaccc attatagtca tttcgtgtct ctttttcccc tttttaatgc aagggaaatt
    51841 actctcctta gatgataatc caaaagctag aaaggtatct caaacctctc tactctccca
    51901 gactagttta caactaaaag gaaaagattc tattttatgg gaaatctagt ttacaagcgg
    51961 taagaacgct aataactaaa cttcttgtac tctttgaaaa tctcttcaaa ccaatgtttt
    52021 gagctatcta tggctagctt cctagtttgc tctttgattt tcattgagta gtaaaactac
    52081 atgtaatggc aatcaagata tcaagaatca tcctactaaa aaaatccata ctttcactat
    52141 aacatagaat aagatatttg actagcattt tcatttgaat ctgaggcctt ttggaaaata
    52201 atttttcaaa acatttccag taacctttgc aaagcccaag ccattgcctt taaccaaaac
    52261 ttggtaccaa ccatttggca gactttctgc cagctgaacg gtttctccag acgcatactt
    52321 gacaaacgct tcttggccaa tttcaaccga ctgttcgacc tgactcggtt tcaaggctaa
    52381 accaagagcg aaactgggct caaagcgttt cttcttaaaa gtgcccagat gcagtccatt
    52441 gcgagcaatc ttgagcttcc ctaaatctgg caaaagttct ggcaagagat aaagctggtc
    52501 tccaaaagtc tgcaagatac ccggtagatt gaccttcaaa tggttttggg caaattcctg
    52561 ccacaaggca acttgttcac ggctgaggtt actcttactt gccttaaatt taggagctgg
    52621 attgttaccc ttaaactgta gatgggcaac aaactgaccc tctcccttaa actgatgagg
    52681 atacatccga gccgtttctg gcaggtcaat accagctacc attccattga tatgctctac
    52741 tggcaacaag tcaaaatcat actcttccag caaccaattg acaatctctt cgttttcctc
    52801 gggtgcccag gtacaagtcg aataaaccag atgaccacct tcagctaaca tggtcactgc
    52861 atcctccaga atttctcttt gcaagctagc acattgactc ggataatcta agctccaata
    52921 gtccatagca tcaggttgct tacgaaacat tccttcacca gagcaagggg catcaagaac
    52981 gattaagtca aaatagcctt taaagacctt gaccaagcgg tcggcagatt cattggtcac
    53041 cacgacattt gtcgctccaa aacgctccat gttttcaacc aaaatcttag cccgtttgct
    53101 tgaaatttca ttggaaataa gtagcccctc ccctgctaga taggctgcca gttgagttga
    53161 tttgcccccc ggtgcagcag ccaagtccaa gaccttcata ccaggactgg gttgggctac
    53221 ttgagccacc atttgagcag caggttcttg cgaataaact aaacctgtag catgctcagg
    53281 cgatttccct gaaaccttcc catagtggcc ccaaagggtt tgagtaatgg catcagaaaa
    53341 ggaaagttgc tcttctttta agggattgac ccgaaaggcc gaaaccgctt cctcctcaaa
    53401 agaggcaaga aaatctcttg cctcatctcc tagtatttct ttatattttt caacaaatcc
    53461 ttctggaaat tgcatttaag ttcttttcct ttcgtaaata taggactgaa tttcctcctg
    53521 catctcaaga ggcaccatca tgacaggctg tctggtttga aaatcaggag cttcaccaaa
    53581 aagggtcaca acccgatagc ccagactttc ccctaaaata ctagctgcgg cataatccca
    53641 tggttgcaga taagtgagat aggtcaacaa acgccctgac aaaatcttgg caaaactaat
    53701 ggccgcactt ccatagacac gaacaccaag aaccgctcgg ctcaaatcag ccagccccca
    53761 ttcattggtt tccagcatac cactattccc tgcaatgaga aaatctccaa gtggtttagt
    53821 tttaaaagga gctaggggcc tatcatttag acaaactgga aattccccac caccgtggta
    53881 acaatcccct ttgaccacat cataaatcag accaaactgt ccctgaccat tttcaaaata
    53941 agccatcata acagcaaaat cttcctgctg ggctacaaaa ttattggtac catcaatggg
    54001 atcaatgacc caaaccttgc cctcttgaac cgaggctcgc agacaacctt cttcagcaca
    54061 aatcttatcc tcaggataac ggaacaaaat ctcaccaacc aagagttcct gaacttcttt
    54121 gtccagtctg gtcaccaaat ctgttggaga ggacttggtt tcaacacgca agtcttcctg
    54181 catatggtca agaatgtact ggcctgcttt cttaacaagc tctttagcaa attcaaattt
    54241 actttccaag agaaatcttt ccttcccctt tttctttggc agcctgaact gctcggtaaa
    54301 gtgaatagcc actaaccgtt tcaaattctc gtcccaaacg tttctcttcg ctcttgcttg
    54361 gcacgaccgc tttgaatccc ttataggagt ccagtaactt tttagcctct actttgcctt
    54421 cataggcagc ttcaacatca ttaaaaaaag aaagcactga agcaagttct tcagtgctcc
    54481 acgacaaatc tagtgggtaa ctatactgtt tgttcattaa ctaataccag ctctcattct
    54541 tgcttctttt agttcttgct tacgataact acgagggaga aaagcacgaa tctcatcttc
    54601 attaaaaccg atttgcatac gcttggcatc aataataatt ggacgacgca aaagactagg
    54661 atactgctca atcaaatgaa gcaattccga taccgaaata ctctctacat caatattcaa
    54721 tttttgaaaa atttttgaac gagttgaaat gatgtcatca gtaccatttt cggtcaagga
    54781 aaggatgtgt tgcaattctt ttcttgttaa aggactggtc ataatattgt gttccacaaa
    54841 gggaacctta tgtttttcta accaggcctt agccttacga catgatgtac agctcggtga
    54901 tagaaatagt gtaatcatgc ttttctcttc ttatctatac tttgctactt ctattataca
    54961 aaaaaataaa gcgcttgact agggattttt agaaaaaaag cctatttttt caagaaaaat
    55021 aggctgtttg cgaacgattg acacaattgg atttggttaa ttcactctta acgatggttt
    55081 taaacgatat atatttttat atatgcaaat taaaaacatc tttcatttca cttcctacga
    55141 cttttcagac acagatagcc aaagaagctt tcatagaggg caaaaaagag gaggaaggca
    55201 tgaagaaaga aggtctctgg caaaatcata ataacaggat ccttggctgg atcaaaaagc
    55261 caggtatcat ctcccacaaa gagaatttga tggaaaagag taaagaattg gtcaaaacca
    55321 atcaaaactc ccccaagtcc aatcatcaca ggtaagacta ctagagtcag gagacttttt
    55381 cgataaagag acaaaaagtc ctttttcaca atcctattga caaagacata gaaacttggc
    55441 agtgtcacta gagctactag ctgaaccaaa tgaaagagat tcttgaccac tgcgaaatgg
    55501 tgcagaccag ctgctgacga acgaaaatca ggcatctgta agacctgact aaaaggattg
    55561 gtcagataat tcatcaagat atgaaaattg tattgaatgg tttctggttt tagatagact
    55621 cgattcgtta agtttagcca ctgaatctcc ataggataga aaatccaagc cagataaatg
    55681 gtcagaagga ttgagaggga gaggagaaag agcatagagc cccaaaaggt caatttagtt
    55741 ttcatcaaaa tcccactccg caaggctaga aaccacatgt gtcggtgcga ttggcaggcc
    55801 agctacttct tctgccttgg taaaacctgt cgtcaccaag agtgttggaa tgccattgtc
    55861 aatcccagcc cggatatcag tcaaataatt gtccccaacc atgattaact cttcacgttc
    55921 caaacctaag tgctcaaccg ccttgtccat aatgatggca ttgggttttc cgatataaac
    55981 cggcttcact cgtgtcgcta cttcaagcag cgtaatcagt gagccagcac ctggcaaaag
    56041 accacgttcc gtcgggatgt tgaggtcagg attggttccg ataaaatggg cacccttttg
    56101 aatagcaaga gttgctgtgg caaatttttc atagtcgact tgccaatcca gaccaactac
    56161 cacgtaggca ggtttttcct tgtcttccac ataaccagcc gccttgatgg cttccttgag
    56221 tcctgcttct ccgacaacat agacggtctt ttcaagcccc aaatcattca tatagtcgat
    56281 ggttgccaaa gtcgctgtgt agacagtcga taggggcgta tcgatattaa aattctgagc
    56341 caacatctcc ttaacactct ctggagtgcg ggttgtattg ttggttacaa agagataggg
    56401 aatgtcccgc ttttgcaatt catgaacaaa agtctctcca gcagggattt ggtctttccc
    56461 cttataaatg gttccgtcta aatcaattaa atagccttta tatttcatct atttctccct
    56521 aagccttttt tatttcttgc caagtaatga ttgcttgggc attgataacc ccatcacttg
    56581 taatttcatg cttgctttcc agtccagtcc gttcaacagc cgatgtaatc accccacctg
    56641 gtcgaacttc cttgacatac ttgaggttga ttttcttggg aatatagtgg gtcaaaaaat
    56701 ccgctcccat gacctcaaaa atccagtcta agtatttact gttattgaca tgaccattca
    56761 tatccaagtc gtaaaaacga acatggtaat ccttgctgat cgactcttcc aaggactcat
    56821 acttcggtcc acggataagt tttttatcaa aatcagactg gtaaggagcc acaatctcag
    56881 gttcaacagc atggactttt cgactgtcgc ggtccatgag aacaaaggtc gccatcatat
    56941 ggatgagctc ctgccccgct tcattataaa tagtaaagcg acggtagcaa aaaagtcgat
    57001 tgtagctcaa ggcttccgtt tcgatggtaa tttcttccgc aaaacgaggc aaacgaacca
    57061 cctcaatatc atattctacg ataatccaga ccagattata gtcttccaaa atggccttat
    57121 cactaactcc cagttcaatc gactgcatcc ctgaaacttg cagtgacagc aaaattacat
    57181 ctggaagttt gatatgaccg ttcatatcag ccatatcaaa aggaattttc attttcattt
    57241 gataagttaa gcccatgatc ctactccaaa ataaatcgtt ccgctacagt atctcccaaa
    57301 aagagacctc tctttgtcat gcgaacgtgg tcaccctcaa tctgcatgag gccttgttga
    57361 accaaatctc tgacaatttc tccataaagt ccagcaaaag actgtccaaa tttttcctca
    57421 aatcgcgcca tggaaacccc ggatttcttg cggagtccca agaacatttc ttcttccatt
    57481 tgctcctttt gactcaggtg atcttctgta atacaagcat tgccttcctc aaccgcactg
    57541 agataatgac gaatgggacc atgattttta tagcgtactc cattgacata accagatgcc
    57601 cctgcaccaa taccatagta ttcagcattg tcccagtaca tgagattgtg acgactttca
    57661 aagccgggtt tggagaaatt agaaatctca taatgctcaa aacccgctcg ctccagctct
    57721 gcaatgatgt actcaaacat ctccgcttct agttcctcct taggcagagg caatttccca
    57781 cgtcgcatcc gattcataaa gaccgtatgg ttttctaaaa tcaaactata caaactcatg
    57841 tggggaatat ccagtccaat ggctttagcc acattttcct ttacttgctc catggtctga
    57901 ccaggcagag cataaatcaa atcaatggag atgttgtcaa aaccagccag tttcaggcga
    57961 tcgatatttt cataaatatc cttctccaaa tgactgcgcc caatcttttt caacatctta
    58021 tcatcaaagg tctggacacc tagcgaaaca cgattgacag ccgaattttt caaaacagct
    58081 atcttatccg catccaaatc gcctggattg gcttcaatgg tcaactcttc taagacagac
    58141 aagtccaagt ttttagtcaa gccattcagt aaaacctcca gttgcggagc cgacagggct
    58201 gtcggtgttc caccaccgat ataaagggtt gacaactttt caatatcata agaacgaaac
    58261 tcttccagca gatgctctaa atagctgtcg actggctgat ttttgatgaa gacctttgaa
    58321 aaatcacaat aataacaaat ctgggtacaa aatgggatgt gcacataggc tgacgttggt
    58381 tttttctgca tagtaattat tataccacaa agactagatt ccagataaaa atcaccatcc
    58441 ccagatacat agtccgtccg gagatggtga tggtttattc ttctgttata tcaatcacaa
    58501 tctcttctga gtcatcaaga gcttcggctt tttcttgcca ttgctccttg agattattta
    58561 attgattttt tgatgcttct gtcgcttgaa aagcatagga tttagcttga gcaagtatac
    58621 tgtccacagt gatttcacct gactcaacct gttcttttgt tttcagaaca aaatctgtag
    58681 cctgctcctt aacttctgtc agtttttcac agacttgctc cttggcatac tccggatctt
    58741 ctctcaaatc atctagaaaa tcttgagcct gactgcaaac ttgtttgccc ttatcacttg
    58801 ttaaaaacaa ggcaagagct gcacctgaaa cggttcctaa aaggattgag gataatttac
    58861 ccataaggat tctccttttt tattttttga aaaatttact tgcaagacga agagctgaca
    58921 gacttgcacc agtcttgagt gtttttgaac cagctgatga agctttcttg ctcaagacac
    58981 gcgcatggtc attgaggtct gaaacagata gagataaatc tgcaacagca ctgaagagtg
    59041 gatcaatcgt agccaccttg acattgatat catctgccaa gacattgacc ttagccaaca
    59101 actcattggt gtgatgcaag gtcacatcca catctgaagt caaggtttta atcgtctttt
    59161 ctgtttcatc gatgacacga ccaagctttt gtacagtaat gatcagatag accaaaaaga
    59221 caatcaaagc tagggcaaca agaatatatg caacttctaa catttagttt tcctcctctg
    59281 taatatagta aggggccttc tttcgatttt gataaataac gatcattata ccgagaccga
    59341 taaggacaac tgacagccat tgggacactc gaaggccgaa gaacatgaga ctatctgttc
    59401 gcataccttc gatgaccata cgaccgaaac cataccaaat caagtaaaag gccgtgatat
    59461 gacctcgtct gagactcttc catttccgtc taaaaatcag aatcaaggca aagccaagca
    59521 gattccatag agactcataa aggaaagtcg gttgacggta gctcccctca atatacatct
    59581 ggtcacggat aaagccaggt agataatcca gattatccac tgttgcacca taagcttctt
    59641 ggttaaagaa attaccccaa cgccccaaac tttgagcaat cataacgcta ggcgccgcaa
    59701 tatctagaaa atcccaagta ttgatgagtt tacggtcagc aaagatatag agcacaagag
    59761 ccccagttat caaaccaccg taaatggcca aaccaccatt ccaaatggca aaaatctctc
    59821 ctaaattctg actatagtaa tcaaatcgga aaataacata gtagagacga gctcctaaaa
    59881 tagccaaggg aaaggctact aagataaaat ctaaaatatc gtctggtatg atcttctttc
    59941 taggtgcttc tttcatggtc aaataaaccg caagaatcaa gcctgtcaca atacataagg
    60001 cataccaacg aatggctagg ggtcctagtt gaatagcaat tggatcaagc attttgcacc
    60061 tcatttcgag cgatgagact tgtcagtcgt tcgtcgaaca aacgggtcgc atcaaagccc
    60121 atttccttgg cacgataatt catggcagct gcctcaatca caacagagat attacgacct
    60181 gttttaactg gaatacgaat acgaggaatg gctacgccag aaacttcaag ttcctctgca
    60241 ttgtttccaa gacgatcaaa ggtcttatgc gtatcgtaat tttccaaata gacagcaagc
    60301 tgaacctgtg aagaatcctt gacagcactc gcaccgtaga gactcataac atcgataata
    60361 ccaaccccac gaatttcaat caagtgtttc aaaatttcag ctggttcacc ccagagagta
    60421 atctcatcct tggcaaagat atcgacacgg tcatctgcta ccaaacggtg accacgtttg
    60481 acaagctcaa gacctgtctc gctcttacca attccactat ctccctgaat caagacgccc
    60541 atcccataaa tatccatcaa gacaccgtgc acactggtac gttctgccaa acgagaatcc
    60601 agatagctag ataactctcc agataaacga ctggtagctg cacggctggt taaaatagca
    60661 atcttacatt ctctagcagc ctttaacatc tcctctggaa ccaccaaacc acgggcaaca
    60721 atgactgctg gtgtctcaga tagaaacatt tttttcaaaa cttcataacg gctgttggaa
    60781 ggcatgctga tcagataaga ccactccttc atccccaaaa gttgaatccg ctctggtgtg
    60841 tagtagtcaa aatagcctgt catttcaaga ccaggtcgcg taatatccgc tgtattgatt
    60901 tccttttcaa gcaactctgg ttcaccatag acaatatcta gtctaagctt ttcaatcact
    60961 tcttttacta aaaccgacat catttcctcc ttcaaatgag ttcttctctc tattatatca
    61021 aattgtagga ggaagtttca gatttttgca ggctttggag tatttattta aaagaaaaag
    61081 gactagattt ctctagcctt tcattccaat tagaattttt tacgtttacg agctacttct
    61141 gatttacgtt tacgttttac agaaggtttt tcatagaatt cacgtttgcg tgtttcttga
    61201 agagtaccag ctttagtaac cgcacgtttg aaacggcgaa gtgcgtcgtc aagagattca
    61261 ttcttacgta ctactgtttt agacattttt ttcactccct tcaagttcga tatctatttc
    61321 attctacacg taaaaggaat aattgtcaag caaaaatgct gtttaatttt gattttttct
    61381 tccatttata caagagttga aagcgttcaa tagaacattg cttttttatt tttcgagtaa
    61441 gctaagcgct tcagcatctg cgatgatggt tacatcaggg tgattttgga ggctacttgc
    61501 aggtaggttc tcagtcactg ggccagatac tgttccggca atggcttctg ctttcgactc
    61561 accgtaagca aaaagaataa tagacttggc atccaaaatg tttttaatcc ccattgaaat
    61621 agcttgggtt gggacgtctt caatcttggc aaagaagcgt gcattggctt cgatagtaga
    61681 ctggtcaagt tctactagat gcgtttgact gtcaaatgga gtaccaggct cattaaagcc
    61741 gatatgccca ttgcgaccga ttcccaagat ttggaggtca actggatggt cagccaaaat
    61801 ttggttgtag cgttcaactt cagcttcagc attgtcctta acaccacgag gcaagaaact
    61861 ttctttaaat ggtttttggt tgaacaagtt ttcttgcatg aagtaacgat aagactgtgg
    61921 attgtcccca tcaaggccca catactcatc aaggttgaca ctggttagat ttgaaaaatc
    61981 aaggtcactc tcaacaattt ccttgtaaaa ttcaagtggg ctacttcctg tcgcaagtcc
    62041 tagagtttga gctccattag ccaatttttc cttcaaaatc tcaaaagcaa cttttccacc
    62101 ttgaacttgg ttttcaactt taataacttt catcttttca tcctccattt ctgtctatat
    62161 ttattatatg gtatagacca atttttgtca agtgaaaacg atttaatttc taaaaaaaga
    62221 gcgtccaaac ttgaaacgtg ttataatgga taggattaga acttagaaag aatgaacaga
    62281 tatatgaata cagctgattt tgatttccac ttgccagagg aattgattgc ccaaacgccc
    62341 cttgaaaaac gtgatgcctc caaactcctc atcgtcaacc gtgagacagg agaaatgcaa
    62401 gataaacact tccactctat tattaatatg ctggaacctg gtgatgccct tgtcatgaac
    62461 gacacccgag ttctccctgc ccgcctctat ggtcaaaaag tggagacagg aggtcatgtg
    62521 gaacttctcc tccttaagaa cactagtgga gacgagtggg aagttctggc taaacctgcc
    62581 aaacgcctca aggtcggtac tcgtatcagc tttggtgatg gccgcctcag cgctgtcgtt
    62641 acagaagaat tgacccacgg gggacgcatt gtccgctttg aataccaagg aattttccta
    62701 gaagtcttgg aaagtctggg agaaatgcct ctgccacctt atatccacga aaaattagat
    62761 gaccgtgaac gttatcaaac cgtctacgcc aaggaaagcg gctctgctgc agcaccgact
    62821 gctggtcttc acttcaccaa agaactgctg gcagaaatcc aagctaaggg tgttcatcta
    62881 gtctatctga ctctccatgt cggactcgga acctttagac ctgtttctgt ggataatctg
    62941 gacgaacacg aaatgcattc agagttctat caactttctg aggaagctgc tgccaccctt
    63001 cgctctgtca aagaaaatgg tggtcgcgtc atcgctgtcg gaaccacttc tatccgcacc
    63061 ttggaaacta ttggttccaa gtttgatggg caaatccaag cagattctgg ttggaccaat
    63121 atctttatca aacctgggta tgagtggaag gtcgtggatg ccttctcaac caacttccat
    63181 ctgccaaaat caactctggt catgttggtt tctgcctttg caggccgtga attagtctta
    63241 gatgcctacc accatgccat ccaagaacac taccgcttct tcagttttgg tgactccatg
    63301 tttatttatt gagaaagaat ttctctaaat cttctaatac caataaatcg ctaagatatt
    63361 atttcaaaga acatctacaa ttgaaactct agctagctgt agaagacgcc tagtacattg
    63421 aaattaaaat gcttccccct agcttcgaaa atattgccat agattgcgtt gactctccaa
    63481 attgattcat ctatatttta tttcagcttc ctatattttc ttcgctgttt gtaaatcaaa
    63541 atgcaagaca catgagtagc accatatttg ttactcttat ctgtcctctc aagagactat
    63601 tatgagttat ttcagaatca ttcactactt tgaccctgac tctccttagt ctcaaaatca
    63661 aagacttata ctcttcaaaa atctcttcaa actgcgtcaa cgtcaccttg gattatatat
    63721 gtgactgact tcgtcagttc tatctacaac ctcaaagcag tactttgagc aacctgcgac
    63781 tagttttcta gtttgctctt tgattttcat tgagtattaa acaaaaagtg aacaaatctg
    63841 aattctaatg tacagaagac taggcttgtt cactttttta tagtcgctat aagatgacct
    63901 tatctatagc tttttatata taattatata taattatata ttcagacata ctattatcaa
    63961 ttttgtcgca gggaggaatc tgttaacgca cccattcacc attatcattg actctatagc
    64021 catctatact tgtattgacc gctaactcac ccgatgcatt tacataatac catttaccac
    64081 caacttggaa ccattgattg actttcatag aaccgttgct gttgaggtag taccatgaac
    64141 cattaacttg tacccaacct gttgccatgg aaccatcagt attataaaaa taccacatac
    64201 cattttcttg tttccagtct gttgttggag caactgcttt agctggttct actgctacat
    64261 ctgttccttg gttagatgta acagatacag gatacgaagg aatagatgat tgctcaggaa
    64321 caacaacttt ttcaggttct ctcgtccctc tccttatacg tctttttacc atctctttag
    64381 taatttgacg agaagtagtt tcttcaattg ttccatcacg ttcatctaca gtatagattg
    64441 tagtaagagt aatttaccaa tttctcctac ttcttgactt ttatcaagag ttgggtcatc
    64501 gagatattct gtttcgattg gaatttcttg gacaagaact tggggcttgg ttcctttttt
    64561 aacaactctt gtttgagagt cttctttttg acttaaagta ctctcagtta cttgtccact
    64621 ctttccatct acatcataag ttatcgttgt aactgttttc ccattctttc ctagagtaat
    64681 ctcttgctcc tgtcctgcag aaaggtcatt gtctgcttca tatttagtag cacatggaac
    64741 aagaacttct tcaaccttgc ttttagctgg aactttgata actgtatccg tggcttgttt
    64801 tctatcaaca gtaacctgtt cggtaacatg accagtctct ggattaacat cgtaggtcct
    64861 tgtcgtagtt acatagccat actctccatc aattgtaaca ggattttcac tacggtcttt
    64921 tgtttcatct ttttcataac gaattcgcgt acttgaaatt ttcttggtta ctaccttagg
    64981 tttagtcgct acttttacaa taataccccc attgtcagcg tcatcatact ctattccctc
    65041 ttctttatct ctagtatcat ctctgacata ttgaatccca tcagcagcat gaacaaaact
    65101 tgtattcaga ttcctcctaa aaataaagtt agcccgatta ccgcagaacc aaaaatcttt
    65161 ccgagtttac gtaatgcata gcgcttatta gtattagatt ttgccattac atcctacttc
    65221 tagtatagca tcttttctat caaacgttaa acaatatact ttatatataa aatatactta
    65281 gaatgatata ttgattattg aactaacact ttaactatat cgtaatcaat ctcatatata
    65341 aaggattgca gacatcttat ctaaatacat gctaatatat ttagatacaa acattccaac
    65401 ttgataattt tcactcatct ttcatcattc cttatacaac tatgcagtat aaatagaata
    65461 gttttctcat cagaatgaga ctattttaat attagataat ttgatagaaa aaacatgaat
    65521 aaaattggtt gaatttaatt ccctaactcg atagaggatt ctattttaaa tttaacaaca
    65581 gctaggatta cactataaaa agtacattac cacctgttcg gctggagaat ctagtcgatt
    65641 agctccctct tagttacaag cttaaagcga ttttttaact aatactaaac cagattaaag
    65701 aaggaactag aacatctgtc ttgtactaat caatatgcta ttttaaagtt tcaaataaac
    65761 ttggaatgct aattattata tttacaataa atagttgact tcgatagaaa cagaaaatag
    65821 aattcaacat tcttccctac ttataccata ataaataaga tccgcaagat agccttttct
    65881 ctctacacca ttaacaatgg tttgtagata ggacattcca gccttttcca tgacacgacc
    65941 tgaagctggg ttgagactgg cataacgtgc tctgaccttt tgaaaacctg cttgagtaaa
    66001 acaaaagtcc aagatagctt tcaaagtctc tgtcatcata ccatttcccc agtaagcctt
    66061 gcctaacaca tagccaattt cacagcttaa atcagcctcg tctatcttaa cgatgctgat
    66121 atctcctatc acttgctctg ggttttcttt tagacaaatg gcccatttat aatagttgag
    66181 attagtatag gaagcaaccc aattgcgaat cgagtttcga gtgatttcga tatcaggatg
    66241 gggatcccag gtaacatagg tcagattctc agcagatgaa gcccaatttt gaaacatggc
    66301 ttctgcatca ctctccacaa atcttcttaa aatcaaacgt tctgtctgta atatttgcgt
    66361 accgatagat ttcatgatgc tacctcactt ccttatttct atgctcgtag taaaaaggct
    66421 caactcagta acagctagaa aacgagtttg tcttctaatt tcttgacgca gtcgctgcct
    66481 ggtttgaaat gcgctctagc aagctttttc aaacctagtc atcgttgcag gggtaagacg
    66541 atgaaatcga actcttcgag ttaccgaatc tgcctcattt tcttatttct aaactcgggg
    66601 ttcaagatcc cccagacctt ctctcacttt cttatttcaa gatgagaggc tgaaaacctc
    66661 cagtggaggt ttttcttgct ttttaatttc agggcaagaa gctaaaaagg tccccggacc
    66721 ttcttcgctt tctcatttct aggctcaggg taaaaacctc caatggaggt ttttactccc
    66781 aaaagtcatc aaatacagtg atgggtaagt ggcgtttgtg ttggcctttg tgccaccagt
    66841 tttcaatggt cgcttgagct tctgggctga ttgttttgcc ttctaggtag tcgtcaatct
    66901 ctgcgtaggt gactccaagt gcgacttcgt cagctaggcc tggtttatct tcttctaggt
    66961 ctgccgttgg gattttttca taaagggctg gctctgcgcc aagtttctgc aagagctgtt
    67021 ttccttggcg tttattgagg cggtaaagag ggagaatatc cgcaccgccg tcaccaaact
    67081 tggtaaagaa acctgtgata ttttccgcgg cgtggtctgt tccaatgacc gctccgctat
    67141 gggaaccagc aagggcatac tgagcaatca tacggcaacg tgccttgata ttccccttgt
    67201 tgaagtctga aacaggacta cctgtcgctt caactgcagc tgtcatggca tcagctgatt
    67261 ccttgatatt cacaaccaag ctgacatctg gctggatgaa ggctagggct ttttgagcat
    67321 ctgcttcatc agcttgcact ccgtatggca ggcggacagc gataaatttg tagctgtcgt
    67381 ctcccgtttc agctcgcagt tcttccatag ctaattgcgc caaacgccct gccaaggttg
    67441 agtcttgtcc cccagaaatc cctagtacaa aggtttttag gaagggatgt tttttcagat
    67501 atctttttaa gaaatcaata gaacgacgga tttcttcctg ggcatcaatc actggtttga
    67561 cacccagctc ttggataatc gtttcttgca aactcattct tcttctcctt caccaagggc
    67621 ttccttgcgc atcttatcaa tcaagtccat cttatcttgc catacatcac gcgccaaatc
    67681 cactggatag tgctgcggat tgagcacacg cttatactca tcccacaact tgtcaaattc
    67741 cttacgggca taatcctgaa tgtcagtcaa actaggcaag ttgtaaacta atattccttc
    67801 tttgaagata tccaccaaga gaggaacggc atcaaaatta cgaaccgtct tcttgatgta
    67861 tgtataggtc ggatggaaca tcttgatttc tgtcatgtcg ctaatatcca caccatcata
    67921 agtgatatag tcgccttctg acttgccttt ttcacgactg gtaatgcgcc acacctgctt
    67981 cttacctggc gtagagactt tttccgcatt attagacagc ttaatcgtat tgcgcatctg
    68041 accagtttca tcttcgattg caacaatctt gtaaactgca ccaagagctg gctggtcata
    68101 agctgtaatc agcttggtac ccacgcccca gacatcaatc ttggcctttt gcatcttgag
    68161 attgaggatg gtattttcat ctagatcatt agaagcataa atcttagcct ctgtaaatcc
    68221 agcctcatcc agttgctgac ggactttctt agaaatgtag gcaatatccc cagagtcaat
    68281 ccgcacaccc ataaagttaa tctgatcacc cagctcacgc gccacctgaa tggcagctgg
    68341 tacaccgatg cgaagggtgt cataggtatc cacaagaaag acacaatttt tgtgggtcgc
    68401 agcgtaagcc ttgaaagctt catagtcatt gccataaacc tgtaccaagg catgggcatg
    68461 ggttcccaaa acaggaatgt caaagagctt acccgcacgc acgttgctgg ttccattggc
    68521 gccaccaatc acagctgcgc gtgttcccca gatggccgca tccatttctt gagcccgacg
    68581 tgtcccaaac tccatcaagg gttcatcttc gataaccgaa cgaatacgag ctgccttcgt
    68641 cgccaccaag gtctggtagt tgacgatgtt caaaagagcc gtttcgacca actgacattg
    68701 ggctagaggt ccttccacct gcacaatcgg ttcattagca aaaaccaaat ccccttcttg
    68761 ggcagaacga acggtcaact ccaacttgaa attgcgaagg taatccaaga acgccccatg
    68821 ataaccaagc gactccaaat aggctatatc actatctgaa aaacgcaagt cttcaagata
    68881 gttcacaatt ctttccaaac ctgcaaaaac cgcatagccg ttcttaaaag gctgttggcg
    68941 gaaatacacc tcaaagaccg ccttcttatt gtgaatccct tggtcaaagt aaacctgcat
    69001 catgttgatc tggtacaagt ccgtgtgcaa tgtcaaacta tcatctggat acatactttt
    69061 cctacttcct tagctagaaa cccatgaaaa ttttcaagaa ctttcatgta ttccaataaa
    69121 ttagtactat tatatcacat tttagctgga ttgagaaaag agtaacaagc tattctccac
    69181 tctccaattc atccatatct tgttcaaatt ttttctgagc ccattcgcca tagctcttaa
    69241 gaccaagatt gccaataaag acccacggaa ggtaaatgac ataagtaatg acccaagcag
    69301 acaggtattt aaaattcaaa ggattgtgct gataaatttc tatgttgaat tgataattct
    69361 gcaacatcaa aagagccgta atagccaagg ttaggaaaaa acaacccaaa atcgtaaaat
    69421 gaaaacgact atagtaggtc actcccagat aacgggcacg gttaaaaaag taaaatgtcc
    69481 ctatgatgat aacgattagc agcatattag aattaaaaag gcttggtgct aatactgaaa
    69541 tgatataaga taggagcgac aaagcgatgc agatatagaa actttcagag cccgctttat
    69601 tgatcagttg ttcttctctt tcgtctagta attgataata ataaaatcta tttttcatct
    69661 tcttcctccc aaaatagttg gtctagggtt ttccctaaac atctgcaaat agactggcag
    69721 agcgagagac tgggattgta ttttcccgcc tctatcaaac caatagtctg gcgtgtcacc
    69781 ccgacagcct ctgccagttg accttgtgtt aaatcacgct ctacccgagc taattttaat
    69841 tttaaatttt tagccacctt cgtcctcctt atagttttaa tactcatcta ctcttaaaaa
    69901 atccaaacta acacaagctg tcagctattt attataaccc cattaatttg cactccagta
    69961 ttatcttcag ccgaaaatca gtattttagg ttgtttgaaa taaacttcct agtttactct
    70021 tttattttta tttttattta gcattagctt tagtaggatc ttttaaacga gtttttattg
    70081 cttatatttc ttgtttagtt tagttggttt catatcacta ttatataatg cttttggaaa
    70141 tgagcctagt ataagtcaaa aacgtgttga atagtgaaag caggagcata ccaatatgac
    70201 tgatttttta ctgtatttta ggtatgcaaa ataatactaa atgaaactaa aatgaatact
    70261 tcttgctttt cttgagagtg aaaaagtaca ataaaatgat tgtagtgatt gccacaagat
    70321 actgactaat agacatcgta tttgaagcca caaatacagg taaagaataa aaaggaagaa
    70381 gttcaattgc tttgataaac aaatcattac tagtattcat catcgtattt aatatattag
    70441 ggataaagag tagtaaagct actaaaatac caagccactt ttcaatttgt ataaatagaa
    70501 aatgtaagat aatagaaaat aaaacaggac taacaaaatt gtattgacta caatagctac
    70561 tataaaatct aatcctaaat cacctggaat acccaattta cttactatga ctagtaacac
    70621 aatgccattc aaagcataaa taatggaagc tgagatggca tatagaaaat tagacaagac
    70681 gtaaatcatc cgattcttct gttgattata aaacaaactt accgtattgt gggaaaaatc
    70741 tctactaatg actcgattag catgtataac agccattaac ataccgagca tagcataaaa
    70801 gtttgaaata tgcttaatac caagcgatgt cccttaagtc tttagaataa agaatgtaag
    70861 caatatcggc ggtataaacg agataagcaa actgagatat attacgcctg atgaatataa
    70921 atttcttata ttttccttaa gaaaattcaa tttatttaat ttcatgatta taaccttcct
    70981 ttattttgaa cgatttaggt aaatatcttt aagcgtctct tttttagttt caaaatcaac
    71041 tactttaata gagttttcgt taaaaaattt aaaaagctca ctactttgaa tattcccaga
    71101 catagtaatt ctcaatccct cttcctgaac aatatcccca aattcttgtt tggtaatgaa
    71161 aatgtctcta tctgtagctg atgaaaaagc tatctcaaac aagaaattat gactatcttt
    71221 tcctactttt tgaaatgtca aaagcccgtt ctccaagaaa agaactctct cacaaatttc
    71281 ttcaatgtct tctaatttat gactcgatat taaaattccc acgttttcat ttaaagctaa
    71341 tttttttaga accgctaaaa ctatttgtga tgattcaata tctaaaccat tagtcggttc
    71401 atctaaaatc aatatatcag gttccgtaac gagagttaga agcaaagcta atttttgttt
    71461 tgtacccaaa gaataggtct ttactttttt attaatagac tgagtcaaat ctaactcttg
    71521 gatcaaactt ctaaatcttt cttgattgta gtcaacacca tataaatttg acaaatattt
    71581 taaattctct aaacctgttt tagataaaaa taattttggt tcttcgatta aatacccaac
    71641 attatcactg cttataatat tacctgaagt cggttgatta ttttgaacaa gaattttcat
    71701 caacgtactc ttaccaactc catttctccc tactagacca acaatttcac ccttgttaag
    71761 tgcaaaattt atatctttga gaatgattga atgaccataa tgcttactta catttttatt
    71821 cctacattaa tattcatggt ttactctttg attcttattg agtttaaaaa gttgcgcctc
    71881 tttttaacaa tattatatca tatatctaac ttgatacaat atataaatgt cattttgtaa
    71941 aattttatta acaaaaaact ctctacaaca aaataataga gagttttctg cttattaatt
    72001 tttttcaaaa tatctaagac atcgcttctt tgtttaattt gataggcttt gatacctaag
    72061 ctccgacttt atcacttaaa aaataaaaaa gacagctaat ctcttgcgca tcaagggatt
    72121 tcgccattct ttaatctcgt ttaagtgttg tatatggagc tttgcataat tttttaaaac
    72181 taaacaacta cattaagcac gaactgtgac gctggttcca tcttccttat aaagattgat
    72241 aagcccctct ttaagagctc gaaccatgtc tcctgattga acgggttgtg ggactttaat
    72301 agtcaatagt tccattggat ttggagcgcg gtcgatttta ttgcctttgg catcatgcaa
    72361 atcttcaata taggtttcaa aatgacggaa acctggacca taaaactcaa cttggtcccc
    72421 ttcgttaatg acgtttcgtt gacgaatagt tgctgtttgt gccgcatcat cataagaaac
    72481 cacttcagcg acaaacttgt actcagggat tttacgacga gcaccaaaca actgctcatt
    72541 ttcagatggt gtaccatagt aaaatcctgt agccagttca cgttgggcaa ccttccacat
    72601 ctcgtccacc aagtcttgtt tgatagcttc aaacttttca ggactttcaa gataggcatc
    72661 cacagccgcc ttgtagcagt tggttactgt taatacatag tgaatagact ccatacgtcc
    72721 ttcgattttt agactgtcca caccattttc aatcatatct ggaatgtggt caatcataga
    72781 catgtcaacg gctgacattg aaaattcttc tggaatctca ccctgcaaac tcttacgttc
    72841 tttcccaaat ggcatatcgt aaaggtcgta tttccaacgg catgactgag aacatccacc
    72901 acggttggca tcacgcatac tctagacgca attctaatct tttagacttt gcataataac
    72961 tgttaaacag ttgaaaatta acccagtttt aacaagtagt caaaatacca gataaaggaa
    73021 aaaaagtctt taaaatctta aaaacgcata gtatcaggta ttgaacaatc ttgatattat
    73081 gcgttttatt gtgggaagat ttacttcatt ttctcttgga attgagtttt ctcctacttc
    73141 aaaactagat gatttgctat ttaaaatagc gttccttatg attttggcaa cctggattaa
    73201 agcgagaaaa acaaaagggg caacttaagc tttctttata ttgcttatat gttagtagtt
    73261 ttagacaaac accacataaa ataggcttat cctgtatcaa agataaggga tacggctcaa
    73321 aagggtgatt ttctaatgaa tcatgacacc gataacaagc atagtacttt ttacaaacat
    73381 agcattgtaa agcaataata tccagcttgc tatggtaatg aatgcatctt gtttgcttat
    73441 ccactaagaa accctgtgcc tctatcattt ccttttcctt tcttaagctc gaactgtgac
    73501 gctggttcca tcttccttat aaagattgat gagtccttct tttaatgcac gaaccatgtc
    73561 tccagcttta acaggttgag gcaccttaat agtcaatagt tccattggat ttggagcgcg
    73621 gtcgatttta ttgcctttgg catcatgcaa atcttcaata taggtttcaa aatgacggaa
    73681 acctggacca taaaactcaa cttggtcccc ttcgttaatg acgtttcgtt gacgaatagt
    73741 tgctgtttgt gtcgcatcat cataagaaac cacttcagcg acaaacttgt actcaggaat
    73801 tttacgacga gcaccaaaca actgctcatt ttcagatggt gtaccatagt aaaatcctgt
    73861 agccagttca cgttgggcaa ccttccacat ctcgtccact aagttttgtt tgatggcttc
    73921 aaacttttca gaactttcaa gataggcatc cacagccgcc ttgtagcagt tggttactgt
    73981 tgatacgtag tgaatagact tcatacgtcc ttcgattttt agactgtcca caccattttc
    74041 aatcatatct ggaatgtggt caatcataga catatcaacg gctgacattg aaaattcttc
    74101 tagaatctca cccttcaaac tcttacgttc tttcccaaat ggcatatcgt aaaggtcgta
    74161 tttccaacgg catgactgag aacacccacc acggttggca tcacgcatac tcatgtggtt
    74221 tgaaagagta caacgtccag agtatgaaat acacatagct ccatggacaa aggcttcaat
    74281 ttcaacatct gtacgtttgc ggatttcagc taattctttc attgaaacct cacgtgctaa
    74341 aacgacacga gtcaagccta gctctttcca gaactcaagg gtttcatagt tagtggcact
    74401 ggcttgggta gaaaggtgga tttcaaggcc tggtgcttca gtcgctgcaa tcataatcaa
    74461 ggctgggtca gatacgataa ctgctgcaat cccgatatca cgcagtttac gaaaccactc
    74521 accagcacca gcttcatttc cttcgtgcat aaccatatta gccgctacat agaccttggc
    74581 accatacttg gccgcaaact gcacgccttc ttccatctgt tcgaaagtaa agtttcccgc
    74641 acggctacga agaccatagg cctgaccacc gataaagaca gcatctgctc catactgaac
    74701 agctaccttt agcttctcta aagtccctgc aggtgataaa acctcaggac gttttaatgt
    74761 ttttgtcatg atttctccta tttgcaatat aaaagtttga catttttcct tctcatttta
    74821 tagtaaataa aggataaaat caagcctaga cggattgttt tgacaattct aatcttttag
    74881 actttgcata ataactatta aacagtcgaa aattacccca gttttaacaa gtagtcaaaa
    74941 agtctaaaaa agtaaaaacc agtatcccaa gatactgact cgtaaaaata tataaattag
    75001 tccttagatg actactccca ctctgttgct cctataatgg aaaaggattg atacagtcta
    75061 gaatatcgct tttttattta ttattctgat ataaaactgt tagaattgat tttttgtttt
    75121 atacccaaat atgaatagag acgtagctaa taagttgaat aacatatttg gaaaaatgag
    75181 aaatagcaat attactagaa cttttagaaa caatcaatat ttaaaaagaa aaaagctcaa
    75241 agaatttatt aaaccatgtt ataatagata tagctataat tttatttata acaagaggat
    75301 agaaatgacc gaattagaaa gaaaaaatcg aaaaattagc taagaaatat tctgataact
    75361 taaacatcaa agttcaagag agagttcgtg aaatggcaaa tgataataag agccattatt
    75421 tgatatacag agttttaggt atttcatttg aagaaggaga aaatatcgat ttgtatcaaa
    75481 ataaaggtcg ttttttatac aaatatgctg gttcattttt agaagaagct gcagtactat
    75541 cctttaacga aaaatttggt acagaaaata cttaaaaagt taacattcct aattctgaaa
    75601 atacaaaacc taagactttt gaaattgatt gtttagtcgg agaaaaacac gcatacgaaa
    75661 taaaatggtg ggatgcaact acagatggag accatataac taaagaacac actagaataa
    75721 aagttattca taacaaagga tatataccaa ttcggttaat gttctactat ccaaatagaa
    75781 ctcaagctat aaaaattcag caaactttag aaacattgta taacggtatt ggagggaaat
    75841 attattatgg agattctgcc tgggaacatt taagagcagt gaccggtatt gatttactta
    75901 gtattctaac agatattgca aataaaaaaa caggggtaaa atcaaaatga cagtattaaa
    75961 aggagataac ttagaaatat taaaaactat tgaatcctca agtattgatt taatctatat
    76021 ggaccctcct ttctttacac agaaaaccca aaaattatct aataacaaaa atattatgta
    76081 ttcattcgaa gatacgtgga cttcgattga ggattacaaa gaatttttgt ctgtaagatt
    76141 agaagaatgc aaaagagtgc taaaaaatag tggcagtatt ttcgttcatt gtgataaaat
    76201 tgcaaatcat catattagat taattttaga taatatcttt ggagcagata tgtttcaaag
    76261 cgaaattata tggaactata aacggtggtc taattcaaaa aagggattat tgaacaatca
    76321 tcaaaacatt tacttttatt caaagtcaaa agattttaaa tttaatacaa tttttacaga
    76381 gtattcttct actacaaata tcgaccaaat actagtagaa cgaaaacgaa atggaaactc
    76441 taaaactata tataaagttg ataataatgg taactatatt ctagcaaaag agaaaaatgg
    76501 agttcccctt tcagatgttt ggaatatacc atttcttaat ccaaaagcta aagaaagagt
    76561 aggttatcct acacaaaaac ctattctgtt attagaacaa attataaaga ttgctactga
    76621 taaaaatgat atagttttag acccgttctg tggaagtgga actactttag tagcctccaa
    76681 gattttgaat agaaattata tggggattga tttatctgag gaagctatca atataactca
    76741 gcaacgtctg gaaaatgtta taaaaacaag ttcaaattta ttgaataaag gaatcgaagc
    76801 atatagaacc aaaactgagg aagaggaaaa cattcttaaa ttattacagg caaaaattgt
    76861 tcaaagaaat aaaggaattg atggtttttt acctaaacat tttcaaaaaa aaccgatacc
    76921 tataaaaatt caaaaaaata atgaatgtct gaatgagagt atctctttat tacagaatgc
    76981 tataaactcc aaaaaacttg attttggagt agttataaaa actcattcag ataatttatt
    77041 atttgatttt gatacgattc ctgaaaatat aatagtagtt gaccattttg aacttacaat
    77101 cgaaaaatgg ttaagtaaaa gtcaacagtt actatagttt gcaaattatt tacagaaaga
    77161 acaaaaaatg ctctgaaaca ctgatttcaa ggcatttttt gttagtttaa aattactccc
    77221 attcttcttt tccaaacgta caatatatcc aaaaccattc aaaatactag attctatttt
    77281 ttataatatc actaaatcca cctaattata ggacgttttc aaatttttag tcccgtccca
    77341 gtcccagtac cggagaaata ttgttttaat ataatatctc tttttgtctt ctaagctctt
    77401 aaaagcaaaa gaacaagtaa agagtcaaga caaggataaa aagtccatat tagggcaaat
    77461 aaaaagcttt aagacagatg acaaatctaa gtcaaataag aaagaccata gcaaaggtgc
    77521 agagagataa atattggcgg tcttcggact gcctttattt ttttatccat ttttcaaatc
    77581 aaatttattc agactatata tgcacatata cacttaaatt catataaaaa catggcttgt
    77641 aaaaaattac tttaatcaca ataatcgcat ttaaaattgt gatgtttgca agctaaatta
    77701 cggacttcac ttggaagttt tcccttgtat cttttataat agatagaaaa tttgctggca
    77761 gatgaatatc caacagattc tgctatctct tttataggta gttcagtgtt taaaagaaga
    77821 gtttcagcta cattcattct ttttctttga gtgtactctg taatgctttg acaatatttt
    77881 tccttaaata aattttttaa tttagtacca ctcattttag atattttttc aagcgtgcct
    77941 tgatttacat tcgttgcaaa atgatcatct aagaatcttg ctacatcttc aagtgcttta
    78001 tcatcatcaa tttcaatttt atattttttt ctatttaagt atgtgtcaat tactatactt
    78061 atccattcat ttgcctttgc tttaaagaaa aaatcagcgg caggagcgtc catcttacaa
    78121 tttaatattt ccattgccac tctttctaag gcctttgtaa gtattatttg attcggttga
    78181 agcaaggttg aataaaaaga ttctggatta atgttaatag atgctaaatg tttttctatt
    78241 agctcttttt taaaaccaat ggaaacagca agataacaac aattctcgtg taataaaaaa
    78301 acaaaatcat cttttatatt atcaaaatca aaagtacata aagagtttgc ggtaatagtt
    78361 tgatacggat taaacttttc tccgtttgca ctgacaatgt aacttgaata aattgaaaca
    78421 tagtctgaca tactataagt gctattttga actacttcct ctttgatata aaaatcatgt
    78481 atatcgataa tgaagctgcc tccttcataa aaccaatata ggccttctgc ataagttgaa
    78541 ttctcttttt tccaacaaaa tgtatgacct gcacttgaat acttctcatt gtttttacat
    78601 tcatctactt tcatataatc ttttacaaaa tcataatatg acataacaca ctatcccttt
    78661 tagacaatat tccaattagc cttattaatt caaaactatt gtattagtaa ttataacaga
    78721 tgtataatag aaaagcaatg atagatatta tcaattaagc gaatttatat ctaaaaggga
    78781 tattaaagaa aggagatatg cttatgaaga tttacaaaaa actatttgct tatgtccaag
    78841 ataagaaata tcttggggtt ttggccataa ttttttctgc tatatctgct gtacttacag
    78901 tatatggata ttatttaatc tacaaatttc tagataagtt aataattaat tcaaacttat
    78961 ccggtgcaga gagtatagca ttaaaatctg ttattacact aacaagtgga gcgatatttt
    79021 attttgtctc aggaatgttt tcacatatct tgggatttag gcttgaaaca aatttaagaa
    79081 aaaggggaat cgatggtctg gaaaaagcaa gttttaggtt ctttgactta aatccatctg
    79141 gtcaaataag aaagatcata gatgataatg cagcacaaac tcatcaggtg gtagctcaca
    79201 tgattccaga tagctctcag gcaatagtca cacctatact tgtccttgta cttggcttta
    79261 tagtaagtat aagagttggc ataattttgc ttgctcttac tataattggt ggcttaattt
    79321 taggggcaat gatgggcgag caagaattta tgaagatata ccaagaatcc ctatctaaac
    79381 taagtgctga aactgttgag tacgtgagag gaatgcaagt tgtaaaaata tttaaagcaa
    79441 atgtagagtc ttttaaaagc ttttataagg cgataaaaga ttactcaaag tatgcttatg
    79501 attattccct atcttgtaaa aggccttatg ttttgtatca atggttattt tttggactga
    79561 ttgcaatttt aattattcct atagtttatt ttatgactag cttagctagc gcagaggtga
    79621 ttttacttga gcttatcatg attttatttt tatcaggagt tctctttgtt tcattcatga
    79681 gaatgatgtg gtactccatg tatatttctc aaggaaatta tgcagtagat actttagagg
    79741 cgctttacga agatatgcaa aaagacaaat tagtgcatgg taatgtcaat aattttaaaa
    79801 actataatat agaatttgag aatgttagct ttgcttataa tgataaagct atcattgaaa
    79861 atttatcctt taatttagaa gaaggaaagt cctacgcact tgtcggttca tctggatcag
    79921 gcaaatcaac agtagcaaaa cttatatcag gtttttacaa tgttaataaa ggaagcataa
    79981 agataggcgg gatagcaata agtgaatatt ctgacgaagc cttaattaaa gccatttcct
    80041 ttgtttttca agattcaaaa ttattcaaga agagcattta tgataatgta gctttagcta
    80101 ataaagatgc gacgaaagat gacgttatga gagccttaaa attagcagga tgcgatttaa
    80161 tattagacaa attcccagaa agagaaaata caatcatagg ctcaaaaggt gtttatttat
    80221 ccggtggaga aaaacaaaga attgcaattg ctagagcaat tttaaaggat tccaaaatta
    80281 ttattatgga tgaagcatca gcatctattg acccagataa cgagtttgaa ttgcaaaaag
    80341 cttttaaaaa tcttatgaag gataaaacag ttatcatgat tgcacacagg ctatctacaa
    80401 ttaaagacct tgatgaaatt attgtcatgg atagtggaaa aattatagaa agagggtctg
    80461 acaaagaatt aatgtcaaaa gatacaaggt ataagagcct gcaagagatg tttaacagtg
    80521 cgaatgaatg gagggtttca aatgaaagag ttttataaaa aaagatttgc tcttacagat
    80581 ggaggagcaa gaaatttaag taaagcaaca ctggcttcat ttttcgttta ttgtataaac
    80641 atgcttcctg ccatattact tatgattttt gctcaggaag ttttggaaaa tatgggcaaa
    80701 agcaatggct tttatatagt attctcagtt ttgactttga tagcaatgta tattttgctt
    80761 tctatcgaat acgataaatt atataacaca acctatcaag aaagtgcaga tttaagaata
    80821 aggacagcgg agaatttatc aaaattacct ctatcttact tttctaaaca tgacatttcc
    80881 gacatttcac aaacaatcat ggctgatatt gaaggcatag agcatgcaat gagccactca
    80941 ataccaaagg tgggcggcat ggtactgttt ttcccattaa tatctgtaat gatgctagcg
    81001 ggcaatgtca agatgggttt agctgtaatt attccatcta ttttaagctt tatatttata
    81061 cctttatcta aaaaatatca ggttaatgga cagaatagat attatgatgt cttaagaaaa
    81121 aactcagaaa gctttcaaga aaatatcgaa atgcaaatgg agattaaagc atataattta
    81181 tcgaaggata ttaaagatga cttatataaa aaaatggaag atagtgagag aatacactta
    81241 aaggcggaag taactacaat tttaactttg tctatatctt caatatttag ctttatatct
    81301 cttgctgttg tgatatttgt cggcgtaaat ctaattatta ataaagagat aaattctctc
    81361 taccttatag gatatttact agctgctatg aagataaaag actctttaga tgcatctaaa
    81421 gagggcttga tggaaatatt ttatttatcg cccaaaatag aaagattaaa agaaattcaa
    81481 aatcaagatt tacaagaagg cgatgactat agcttaaaaa aatttgatat tgatctaaaa
    81541 gatgttgagt ttgcctacaa taaagacgca aaagttttaa atggtgtaag ttttaaagct
    81601 aagcagggag aggtcactgc tttggtaggt gcaagtggct gcggtaaaac aactatcttg
    81661 aaacttatat caagacttta tgattatgac aagggacaaa tcttaatcga tggcaaagat
    81721 ataaaagaaa tatcaacaga atctcttttt gataaggtct ctatagtttt ccaagatgtg
    81781 gttctcttta atcaaagcgt tatggaaaat attagaatcg gtaagcaaga tgcaagtgac
    81841 gaagaggtta aaagagcagc aaaacttgca aattgcacag attttataga aaaaatggat
    81901 aaaggtttcg atacagttat tggtgaaaac ggagctgagc tatcaggagg agaaagacaa
    81961 agattatcaa tagccagagc cttcttaaaa gatgcgccga tattgatctt agatgagata
    82021 acagcaagcc ttgatgttaa caacgagaaa aagattcaag agtctttaaa taatttagtt
    82081 aaagataaaa ctgttgtaat catttcacat agaatgaaat ccatagaaaa tgcagacaag
    82141 atagtagttc ttcaaaacgg aagagtagaa agcgaaggta agcatgaaga gcttttacaa
    82201 aaatcaaaaa tttacaaaaa tttaatagaa aagacaaaaa tggcagaaga atttatttat
    82261 taggaggact acaatggata ataaaaaatt aaaagtaaaa gatttagtaa gtatcggtgt
    82321 ttttggcgta atttattttg ccttcatgtt tggagttggt atgatgggct tgattccaat
    82381 attgttctta atatacccga cagtattagc catagttgca ggaactgttg ttatgttatt
    82441 tatggctaag gttcaaaagc catgggcact atttatattt ggtatgatat caccacttgt
    82501 gatgtttgca gctggtcata cctacgtagt tgtggtttta tcacttatag taatgataat
    82561 agcagaatta attagaaaga ttggtaatta taattcattt aaatacaata tgctttctta
    82621 tgcaatcttc agcacatgga tatgtagctc tttaatgcaa atgcttttag caaaagaaaa
    82681 atatatggag tggtctttga tgacgatggg aaaagattat gttgatgtat tagaaaagtt
    82741 aataacttat cctcacatgg ctttaatagc cttaggtgct ttcttaggag gaattcttgg
    82801 agcatatata ggcaaggctc tattgaaaaa acacttttca aatggattat attgtgtggg
    82861 atactttact ccttgcctaa ttttatggtg ttatctgaat taaaccctat agttaagatg
    82921 tttttgagta tacctattgt tattagaatg tttattttac catttatggc agcaagcttt
    82981 atgataaaga cctcggatgt aggcgcaata atttcatcga tggataagct taagatttca
    83041 aagaatgtat ccatacctat tgcggttatg tttagattct tcccatcttt taaggaggag
    83101 aagaaaaaca tcaaaatggc tatgagagta agagggataa attttaaaaa cccagtcaaa
    83161 tatcttgaat atgtttctgt gccactactc attatatcat ctaatatatc agatgacatt
    83221 gcaaaagcgg cagaaacaaa ggcaatagaa aatccaattg ccaagaccag atacattcgc
    83281 gtaaagatac agctaattga ttttgtttat gttttagcgg ttgctggact tattgtggga
    83341 ggcttaatat ggttgaaata aaaaatttaa gtcttgatta tggtgaagag catatattag
    83401 atgatatatc actatccata gccgagggag agtgcgtgct atttacagga aaaagtggaa
    83461 gtggtaagtc atctttaata aattcaatca atggactagc tgtaaggtat gataacgcaa
    83521 agacaaaggg cgaaataatt attgatggta agaatataaa aaatttggaa ctttatcaaa
    83581 tctcaatgct tgtttcaact gtttttcaaa atcctaagac atattttttt aatgtcaata
    83641 cgacattaga attattattt tatttggaaa atatcggtct tgcaagagaa gagatggaca
    83701 ggcgtttgaa ggatatactt gagatattcc cgataaaaaa tcttttgaac agaaatatat
    83761 ttaatctatc cggcggtgaa aaacaaattc tttgcattgc agcttcttat atagcaggta
    83821 caaagattat agttatggat gagtcttcat cgaatttaga tattaaaagc ataagtgttt
    83881 tggcaaagat gctaaagata ttaaaagaga aaggcataag cataattgtt gcagagcata
    83941 gaatttatta tttgatggac atagttgacc gtgtattttt aatagataaa ggaaagctta
    84001 aaaaaactta tactagaagt gaatttttaa agctagataa aaatgaatta aatgctttaa
    84061 gtttaagaga taaagaatta agtaaattaa aagttcctta tttaaaagaa ggtggagagt
    84121 atcagataaa aaatcttagc tacaaattta ctgatgatga gtgtttaagc ttaaaagata
    84181 tttcgttcaa gcttgggaaa atttatggca taataggatc caacggacga ggaaaatcaa
    84241 cgcttttaag atgtttaata ggtcttgaga aaaaatcaaa agaagaaatt tattttaagg
    84301 gagagaagct atctaaaaaa gaaagactca aaaactcttc acttgttatg caagatgtaa
    84361 atcatcaatt attcacagat gaagtattca acgagcttag attaggagta aagaattttg
    84421 atgaagaaaa ggcgaaaatc attttaaaag atttaggcct ggacgaattt attgaaaggc
    84481 atccgatgag tttatcagga gggcaaaagc aaaggcttgc aatagcatct gttatgtgca
    84541 agacttctcc atttatcttt tttgacgaac cttcaagtgg tatagattat tccaatatga
    84601 taaaaatatc tgaactgatt aataagtata aaaccatgga taaaataatt tttattgttt
    84661 cccatgatat agaattttta aatgaagtgg cagatgaaat ttttgaattg taaaatagag
    84721 tctgttaaac atgtctatag aactggtaaa ttgtctaaaa atttaaaaga tactaacaca
    84781 tattgatatt tattagaaaa ttaacttcag gatttttaca gtatttataa actttataat
    84841 atgtatttat agtattaata atttccatat ctttagggaa attatttatt tacttaccat
    84901 aattaaagag atgagagata aatatcggca gtcttcggac tgcctttatt tttttactta
    84961 atttaatgca gtaaatcata cgattgaaag gaatctgcgt tatatgctat aattaggtag
    85021 ttaatctata catttagaaa ggtaaaatat agaattggag aatgatatgg gattttcata
    85081 taataaattg taacc
//
LOCUS       DAVBGY010000002        79209 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVBGY010000002 DAVBGY010000000
VERSION     DAVBGY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 79209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79209
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.002"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            1..235
                     /locus_tag="VQ620_000090"
                     /pseudo
     CDS             1..235
                     /locus_tag="VQ620_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            232..450
                     /locus_tag="VQ620_000091"
     CDS             232..450
                     /locus_tag="VQ620_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2762794.1"
                     /translation="MSISPRFEALEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(507..791)
                     /locus_tag="VQ620_000092"
     CDS             complement(507..791)
                     /locus_tag="VQ620_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HET2762795.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(845..1780)
                     /locus_tag="VQ620_000093"
     CDS             complement(845..1780)
                     /locus_tag="VQ620_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET2762796.1"
                     /translation="MEICQQILEKIKEYNTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNVTDAETAAIVGAPGRIDSVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            1884..2327
                     /locus_tag="VQ620_000094"
     CDS             1884..2327
                     /locus_tag="VQ620_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HET2762797.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            2443..2709
                     /locus_tag="VQ620_000095"
     CDS             2443..2709
                     /locus_tag="VQ620_000095"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET2762798.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSYILKRSRDYQDQNIK"
     gene            2706..3080
                     /gene="crcB"
                     /locus_tag="VQ620_000096"
     CDS             2706..3080
                     /gene="crcB"
                     /locus_tag="VQ620_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET2762799.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            3074..3403
                     /gene="crcB"
                     /locus_tag="VQ620_000097"
     CDS             3074..3403
                     /gene="crcB"
                     /locus_tag="VQ620_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET2762800.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            3522..3869
                     /gene="rplS"
                     /locus_tag="VQ620_000098"
     CDS             3522..3869
                     /gene="rplS"
                     /locus_tag="VQ620_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET2762801.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            3912..3983
                     /locus_tag="VQ620_000099"
     tRNA            3912..3983
                     /locus_tag="VQ620_000099"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3945..3947,aa:Arg,seq:cct)
     gene            complement(4150..5148)
                     /locus_tag="VQ620_000100"
     CDS             complement(4150..5148)
                     /locus_tag="VQ620_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HET2762802.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNELECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLTVVELKTILSKHN
                     LATSGKKAELITRIINNVNIDNLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDISMED
                     YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHA
                     LIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECYSPLNNLELDDFE"
     gene            complement(5191..5997)
                     /gene="yidA"
                     /locus_tag="VQ620_000101"
     CDS             complement(5191..5997)
                     /gene="yidA"
                     /locus_tag="VQ620_000101"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HET2762803.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRPMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(6010..7314)
                     /locus_tag="VQ620_000102"
     CDS             complement(6010..7314)
                     /locus_tag="VQ620_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET2762804.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTVTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPVENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKNFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESQNPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            7386..7763
                     /locus_tag="VQ620_000103"
     CDS             7386..7763
                     /locus_tag="VQ620_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HET2762805.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELMMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            7854..8186
                     /locus_tag="VQ620_000104"
     CDS             7854..8186
                     /locus_tag="VQ620_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HET2762806.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            8198..9769
                     /gene="ffh"
                     /locus_tag="VQ620_000105"
     CDS             8198..9769
                     /gene="ffh"
                     /locus_tag="VQ620_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET2762807.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(9964..11247)
                     /locus_tag="VQ620_000106"
     CDS             complement(9964..11247)
                     /locus_tag="VQ620_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HET2762808.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(11501..12214)
                     /gene="rsmG"
                     /locus_tag="VQ620_000107"
     CDS             complement(11501..12214)
                     /gene="rsmG"
                     /locus_tag="VQ620_000107"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET2762809.1"
                     /translation="MKPETFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            12308..12868
                     /locus_tag="VQ620_000108"
     CDS             12308..12868
                     /locus_tag="VQ620_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET2762810.1"
                     /translation="MTWIILGVLALVVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTSEEEKSVPKVDFSGLGD"
     gene            12870..13769
                     /gene="htpX"
                     /locus_tag="VQ620_000109"
     CDS             12870..13769
                     /gene="htpX"
                     /locus_tag="VQ620_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET2762811.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(13813..15372)
                     /locus_tag="VQ620_000110"
     CDS             complement(13813..15372)
                     /locus_tag="VQ620_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2762812.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKTAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLAQELQLTVRGQEKIGIIGPNGVGKSTLLAKLQQLLNDKREISLGFMPQDYHK
                     KLQLDLSSIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(15611..15928)
                     /locus_tag="VQ620_000111"
     CDS             complement(15611..15928)
                     /locus_tag="VQ620_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET2762813.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSPLIGLGSLKLNDQARTAWRNYDKIHYEHVKHVLSLYGPV
                     FGF"
     gene            16107..16649
                     /locus_tag="VQ620_000112"
     CDS             16107..16649
                     /locus_tag="VQ620_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET2762814.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            16649..17278
                     /gene="nth"
                     /locus_tag="VQ620_000113"
     CDS             16649..17278
                     /gene="nth"
                     /locus_tag="VQ620_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET2762815.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            17489..18010
                     /gene="pyrR"
                     /locus_tag="VQ620_000114"
     CDS             17489..18010
                     /gene="pyrR"
                     /locus_tag="VQ620_000114"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET2762816.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            18029..18952
                     /locus_tag="VQ620_000115"
     CDS             18029..18952
                     /locus_tag="VQ620_000115"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET2762817.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            19002..20081
                     /gene="carA"
                     /locus_tag="VQ620_000116"
     CDS             19002..20081
                     /gene="carA"
                     /locus_tag="VQ620_000116"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET2762818.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            20393..23569
                     /gene="carB"
                     /locus_tag="VQ620_000117"
     CDS             20393..23569
                     /gene="carB"
                     /locus_tag="VQ620_000117"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET2762819.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(23844..24653)
                     /locus_tag="VQ620_000118"
     CDS             complement(23844..24653)
                     /locus_tag="VQ620_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2762820.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDQFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(24655..25458)
                     /locus_tag="VQ620_000119"
     CDS             complement(24655..25458)
                     /locus_tag="VQ620_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2762821.1"
                     /translation="MKQLTVEDAKQIELEILNYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRLVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSATFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(25468..26955)
                     /gene="tacF"
                     /locus_tag="VQ620_000120"
     CDS             complement(25468..26955)
                     /gene="tacF"
                     /locus_tag="VQ620_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HET2762822.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGDILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            27291..27998
                     /locus_tag="VQ620_000121"
     CDS             27291..27998
                     /locus_tag="VQ620_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET2762823.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDSIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            28003..29025
                     /locus_tag="VQ620_000122"
     CDS             28003..29025
                     /locus_tag="VQ620_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET2762824.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            29042..29911
                     /gene="licA"
                     /locus_tag="VQ620_000123"
     CDS             29042..29911
                     /gene="licA"
                     /locus_tag="VQ620_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HET2762825.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            29901..30779
                     /locus_tag="VQ620_000124"
     CDS             29901..30779
                     /locus_tag="VQ620_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET2762826.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            30791..31480
                     /locus_tag="VQ620_000125"
     CDS             30791..31480
                     /locus_tag="VQ620_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET2762827.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQNIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     HKLEEILKNEN"
     gene            31578..32426
                     /gene="dprA"
                     /locus_tag="VQ620_000126"
     CDS             31578..32426
                     /gene="dprA"
                     /locus_tag="VQ620_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HET2762828.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            33310..34353
                     /locus_tag="VQ620_000127"
     CDS             33310..34353
                     /locus_tag="VQ620_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HET2762829.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGHNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFYTESEEQ"
     gene            34520..36607
                     /gene="topA"
                     /locus_tag="VQ620_000128"
     CDS             34520..36607
                     /gene="topA"
                     /locus_tag="VQ620_000128"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET2762830.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            36828..37187
                     /locus_tag="VQ620_000129"
     CDS             36828..37187
                     /locus_tag="VQ620_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HET2762831.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKIYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            37280..37912
                     /locus_tag="VQ620_000130"
     CDS             37280..37912
                     /locus_tag="VQ620_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HET2762832.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGTLTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQLGVTQLHGTKVVF"
     gene            37956..38612
                     /locus_tag="VQ620_000131"
     CDS             37956..38612
                     /locus_tag="VQ620_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET2762833.1"
                     /translation="MFEIFKSYQFNQEKAHAFGFVVNSGVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETDDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            38631..39263
                     /locus_tag="VQ620_000132"
     CDS             38631..39263
                     /locus_tag="VQ620_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET2762834.1"
                     /translation="MNFQLAKYSLLKKFSENIEFTTPGEYGAIFKYLIENVKTDRQII
                     CSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            39291..39725
                     /locus_tag="VQ620_000133"
     CDS             39291..39725
                     /locus_tag="VQ620_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HET2762835.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENACEMGRSVSYHDMPSV"
     gene            39737..40774
                     /gene="leuB"
                     /locus_tag="VQ620_000134"
     CDS             39737..40774
                     /gene="leuB"
                     /locus_tag="VQ620_000134"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET2762836.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADVILLAAIGSPQYDGAAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     VMLMISNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIYGSA
                     PDIAGQGIANPTSMILSVAMMLRDSFGRYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            40771..41040
                     /locus_tag="VQ620_000135"
     CDS             40771..41040
                     /locus_tag="VQ620_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HET2762837.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRTWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            41044..41403
                     /locus_tag="VQ620_000136"
     CDS             41044..41403
                     /locus_tag="VQ620_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET2762838.1"
                     /translation="MAGSSREHAAWALANYGFKVVIAGTFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDHVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            41565..42133
                     /locus_tag="VQ620_000137"
                     /pseudo
     CDS             41565..42133
                     /locus_tag="VQ620_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007122522.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            42227..42292
                     /locus_tag="VQ620_000138"
                     /pseudo
     CDS             42227..42292
                     /locus_tag="VQ620_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            42455..44395
                     /locus_tag="VQ620_000139"
     CDS             42455..44395
                     /locus_tag="VQ620_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HET2762839.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELMSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNESDENDENDDADY"
     gene            44382..45734
                     /locus_tag="VQ620_000140"
     CDS             44382..45734
                     /locus_tag="VQ620_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HET2762840.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKKQKILETINQEIKTGNVIDFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSDYSAFCKMMENSEENFKAIIDEEVGRK
                     "
     gene            45909..46895
                     /locus_tag="VQ620_000141"
     CDS             45909..46895
                     /locus_tag="VQ620_000141"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HET2762841.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKQMHDQGLIASISVGVKDYEY
                     DFVSQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            46959..47030
                     /locus_tag="VQ620_000142"
     tRNA            46959..47030
                     /locus_tag="VQ620_000142"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46992..46994,aa:Arg,seq:ccg)
     gene            47213..47911
                     /locus_tag="VQ620_000143"
     CDS             47213..47911
                     /locus_tag="VQ620_000143"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET2762842.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            47902..51441
                     /gene="smc"
                     /locus_tag="VQ620_000144"
     CDS             47902..51441
                     /gene="smc"
                     /locus_tag="VQ620_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET2762843.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKSEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAITVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDAVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLRSEEAALKTLQDQMATLTERLEVIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQDRLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRYLQSQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKDRFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            51438..52232
                     /locus_tag="VQ620_000145"
     CDS             51438..52232
                     /locus_tag="VQ620_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2762844.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGETWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            52232..53050
                     /locus_tag="VQ620_000146"
     CDS             52232..53050
                     /locus_tag="VQ620_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2762845.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKATLKAASDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGHEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            53054..54274
                     /gene="ftsY"
                     /locus_tag="VQ620_000147"
     CDS             53054..54274
                     /gene="ftsY"
                     /locus_tag="VQ620_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET2762846.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAVLEESQDSEPAVGVSQEEIEELPEEEKLEHEGTIEENNSEVLEAERPQTEETVQ
                     EKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEELLIMSDVGVQVASNLTEELR
                     YEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQDNLTVMLFVGVNGVGKTTS
                     IGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVDVPVVTGPEKADPASVVFDG
                     MERAVAEGIDILMIDTAGRLQNKDNLMTELEKIGRIIKRVVPEAPHETFLALDASTGQ
                     NALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREELNIPVKLIGFGEKIDDIGEF
                     NSENFMKGLLEGLI"
     gene            complement(54320..55807)
                     /locus_tag="VQ620_000148"
     CDS             complement(54320..55807)
                     /locus_tag="VQ620_000148"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET2762847.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPAIFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRFE"
     gene            complement(55956..56696)
                     /locus_tag="VQ620_000149"
     CDS             complement(55956..56696)
                     /locus_tag="VQ620_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2762848.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDNLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(56696..58861)
                     /locus_tag="VQ620_000150"
     CDS             complement(56696..58861)
                     /locus_tag="VQ620_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET2762849.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            59034..59987
                     /locus_tag="VQ620_000151"
     CDS             59034..59987
                     /locus_tag="VQ620_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2762850.1"
                     /translation="MKNKRIFKDFQASKMNLNLYTSPLLAFVFVFIGEFVAYTLYGIS
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENCLSNLLKGFSLGLALFLLTLLSLVVLGQYRLESIHLNPYYLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            60064..62052
                     /gene="uvrB"
                     /locus_tag="VQ620_000152"
     CDS             60064..62052
                     /gene="uvrB"
                     /locus_tag="VQ620_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HET2762851.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            62062..62625
                     /locus_tag="VQ620_000153"
     CDS             62062..62625
                     /locus_tag="VQ620_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2762852.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNCLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            62591..63079
                     /locus_tag="VQ620_000154"
     CDS             62591..63079
                     /locus_tag="VQ620_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2762853.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWREAYDDLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAIGDLKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            63153..63620
                     /locus_tag="VQ620_000155"
     CDS             63153..63620
                     /locus_tag="VQ620_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2762854.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(63617..64132)
                     /locus_tag="VQ620_000156"
     CDS             complement(63617..64132)
                     /locus_tag="VQ620_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HET2762855.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(64144..64671)
                     /locus_tag="VQ620_000157"
     CDS             complement(64144..64671)
                     /locus_tag="VQ620_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2762856.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(64949..65512)
                     /locus_tag="VQ620_000158"
     CDS             complement(64949..65512)
                     /locus_tag="VQ620_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2762857.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(65496..66047)
                     /gene="coaC"
                     /locus_tag="VQ620_000159"
     CDS             complement(65496..66047)
                     /gene="coaC"
                     /locus_tag="VQ620_000159"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HET2762858.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(66059..66748)
                     /gene="coaB"
                     /locus_tag="VQ620_000160"
     CDS             complement(66059..66748)
                     /gene="coaB"
                     /locus_tag="VQ620_000160"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HET2762859.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            66978..68648
                     /locus_tag="VQ620_000161"
     CDS             66978..68648
                     /locus_tag="VQ620_000161"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET2762860.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            68976..70151
                     /gene="mutY"
                     /locus_tag="VQ620_000162"
     CDS             68976..70151
                     /gene="mutY"
                     /locus_tag="VQ620_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET2762861.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     LIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            70206..70910
                     /locus_tag="VQ620_000163"
     CDS             70206..70910
                     /locus_tag="VQ620_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2762862.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            70903..72252
                     /gene="vicK"
                     /locus_tag="VQ620_000164"
     CDS             70903..72252
                     /gene="vicK"
                     /locus_tag="VQ620_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HET2762863.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            72254..73063
                     /locus_tag="VQ620_000165"
     CDS             72254..73063
                     /locus_tag="VQ620_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET2762864.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            73154..73396
                     /locus_tag="VQ620_000166"
     CDS             73154..73396
                     /locus_tag="VQ620_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HET2762865.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            73398..73652
                     /locus_tag="VQ620_000167"
     CDS             73398..73652
                     /locus_tag="VQ620_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET2762866.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            73699..74472
                     /locus_tag="VQ620_000168"
     CDS             73699..74472
                     /locus_tag="VQ620_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HET2762867.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFTTKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLAKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPASIFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSSQRILLEKQSKKIEELHKMIQDLIEKT"
     gene            complement(74732..75718)
                     /locus_tag="VQ620_000169"
     CDS             complement(74732..75718)
                     /locus_tag="VQ620_000169"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HET2762868.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGLYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            75912..78380
                     /gene="gyrA"
                     /locus_tag="VQ620_000170"
     CDS             75912..78380
                     /gene="gyrA"
                     /locus_tag="VQ620_000170"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET2762869.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIALRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEE"
     gene            78380..79123
                     /gene="srtA"
                     /locus_tag="VQ620_000171"
     CDS             78380..79123
                     /gene="srtA"
                     /locus_tag="VQ620_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HET2762870.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT        24162 a        14128 c        17358 g        23561 t
ORIGIN      
        1 ccttaaagat tccgactaat attggctttg catttattcc tccatacaca ccagagatga
       61 acccattgaa caagtgtgga aagagattcg taaacgtgga tttaagaata aagcctttta
      121 aactttggaa gatgtcatga atcaactcca agatgttata caaggactgg agaaggaggt
      181 gataaagtcc atcgttaatc ggagatggac tagaatgctt tttgaaagca gatgagtata
      241 agtcctcgtt ttgaagcctt ggaacaggcc attgcctcca aagatttgga aaaagtccga
      301 gaagctttta agaaaatgaa ttccacttgg accatcaatg aaagtgtggt tcgtgacaat
      361 agtacagctc attatggacg tgttgaaaca gctatttcct tcctgcctag tagcatggaa
      421 accgaaccta cagatgagtc tggaacatga aaacagcagt tgatttcaag aaacaaaaaa
      481 gaacagtcga aactgttctt ttatcattat ttgagaccgt attttttgtt gaaacgatcc
      541 acgcgtccat ctgcttgagt gaacttttga cgtccagtgt agaatgggtg tgagtctgat
      601 gaaatttcca cacggatcaa tgggtaagtt tcgccttcga actcaactgt ttcgttagag
      661 cgttttgttg aaccgctaag gaattggtaa ccagtagttg tgtccatgaa gacaactggg
      721 cgatattctg gatggatatc ttttttcatt ttgcaatatt tcctttctgc catggtctct
      781 ttgcgagcca tagattgtta ccatactagt ctatcagatt ctgaaaagtt tggcaagtat
      841 tttatcagtt tttaagcaag ttttttaact tttggtagat gatttcattt tcttctaggc
      901 tataggaatt agcaccactt gctagagggt ggcctccacc atcatgctcc ttggcgattt
      961 cattgatagg atggatttta ctgcgtaagc gaacgcggta gtggccatca gcctgttcca
     1021 caaaaattcc ccagagacta acactatcaa tacgcccagg tgcacctaca atggccgcag
     1081 tttcagcatc ggttacattg tattgtttca agattttctg actcaggata acgcgagcag
     1141 caccattttc atccacttcc agatggtcgt agatgtagcc ttgcagttta gcaattttgt
     1201 agctcatagt gtccattttg cgagtgagag ccgcaaagtc aaagttatgt tctctcaaat
     1261 aagcagccag gcgaagagtc cgtgcagtgg tagaagggta gaggaagcga cctgtatcac
     1321 cgacaattcc tgcaaagagc aactcagcat cgcgatctgc caaggctagt tgggttgttt
     1381 gggcaaatag ggtaatcatc tcgctagcgc tacttgaact agtatcgacc caagacaggt
     1441 caccgtatac atcatcattt ggatggtggt caatcttaat gagaaaatca ccttgactat
     1501 agcgcttatc atcgatacga gcagtattag ctgtatcaca gacgatgaca agtgcgcctt
     1561 ggtaggctct atcttcaaca agatccatct cagccatcca agtaagagtt ggttcatcaa
     1621 aaccgacggc tttgatggtt ttttctggga aatgatgttc cagcaaggct ttcaatccca
     1681 cctgacttcc caaggcatca gggtctggtt tcatatgacg atgaatgata atggtgttgt
     1741 attctttaat tttttctaaa atttgttggc aaatctccat aaataacctc aattctttct
     1801 catttcattg tagcacaaga atttttattt ttaaaatgaa aaagatgtga tataatggag
     1861 aaagataaat tgaggaggac gatatggcat tagcaaaaat tgtatttgcc agtatgaccg
     1921 gtaataccga agaaattgca gatattgtag cagacaaatt acgtgacttg ggcttggatg
     1981 ttgatgttga tgaatgtaca actgttgacg cttcagactt cttggaagca gacatcgcta
     2041 tcgttgcgac ctatacttat ggtgatggtg aattgccaga tgagatgatg gacttctacg
     2101 aagacctagc agatctcaac ttgaatggca aaatctacgg agtggtcggc tcaggagata
     2161 ccttctacga tgaattctgt aaggctgttg atgactttga ccgtgtcttt gtgtcaacag
     2221 gagcagaaaa aggttcagag tgtgttaaag ttgatctttc tgccgaggaa gaagatattg
     2281 aacgcttgga acaattcgca gaagaattgg ctgctaaagt aggataagca taaaagtgcg
     2341 ctgatgtaat caatcagtgc atttttctta tcgagagttg ggattttact agcctaattg
     2401 atggcttttt gatacaataa ttcaaataaa ggaggagact gtatggattt agatattatt
     2461 cgtcaagaaa tcgatcaaat cgacgaccaa attgtcaaac tgttagaaga acgaatgcat
     2521 ttagttgagg gggtggttgc ttataagaaa gcttcaggta aaccgatttt agatactaag
     2581 agagaagaag ttatttttga aaaggttagg agtcgtgtag aggacaagcg ctatcaggag
     2641 actattgtcg cgactttttc gtacatactc aaacgttcgc gtgattatca ggatcaaaat
     2701 atcaaatgaa aaaagaacaa ttttatccgc taggaatttt tctagctgct atgttgggcg
     2761 gacttgtccg atatctagtt tccacctggt taccagccag tccagacttt ccttggggca
     2821 ctctctttgt caactatctg ggaattttct gcttgatttt tcttgtcaag ggctatctgg
     2881 tctataaggg gactagtaag ggcttgattt tagcactggg gacgggtttt tgcggaggtt
     2941 taacaacttt ttccagccta atgcttgata ctgtgaagct gcttgataca gggcgttatt
     3001 ttagtttagt cctgtatttg cttttgagta tcggtggagg cctgctttta gcttactatt
     3061 tggggaggaa gaaatggtaa tcgtctatct tgcaatcgcc tgtggcttgg gagcactcgt
     3121 acggtatttc ttttctcgct ataatcaagc ttctaaattg ccactcggaa cgctcatagc
     3181 taatctttta ggttgttttt taattggagt attctacaat catgtagagt ctaaggaagt
     3241 atatgctatt ctagcaacag gattttgtgg cggtttaaca actttttcga ccttgaatga
     3301 cgaacttcaa agactgctaa gtgataagaa ggtcttttat tcttacctga ctttgactta
     3361 cataggtggt ttggttgcga tttttttagg aattttgcta taaatttctt tacttttttt
     3421 tagaaatttg gtaaactata tcttgtgtgt aatggacgca caaaaataca gtttatccgc
     3481 tgaggaagat tcctcaagat tgacaaagat aggagaataa aatgaatcca ttaatccaaa
     3541 gcttgactga aggtcaactt cgtacagata tcccatcatt ccgtcctggt gacactgttc
     3601 gtgtacatgc gaaagttgtc gaaggtaacc gtgaacgtat ccagattttt gaaggtgttg
     3661 ttattgcacg taaaggtgct ggcatctctg aaaactacac agttcgtaaa atctctaacg
     3721 gtgtaggtgt tgagcgtatc ttcccaatcc acactccacg tgttgaaaaa atcgaagttg
     3781 tacgttacgg taaagtacgt cgtgcgaaat tgtactactt gcgtgctctt caaggtaagg
     3841 cagctcgtat caaagaaatc cgtcgttaat tcgactaaaa aactctgctt tttcagcaga
     3901 gtttttatct agctccctta gttcaatgga tataacaact ccctcctaag gagtagttgc
     3961 aggttcgatt cctgcagggg gcatgtaatt agatatcaaa gaagtttgtc aaagagtctt
     4021 tgatattaaa agaacgcata atatagggga ttgtctccat aatattatgc gttttattat
     4081 tgaaaaattt gggcaatcat cagataaaat tgagactata tcgagtatat aaatgtttgt
     4141 atagtcaatt tattcaaaat cgtcaagttc taaattattt aaaggactat aacattcata
     4201 tttctttaag taattgttta tttcttcagc agtggaacga ggtaaattaa ctcttaaata
     4261 gtcaatatct tccttagata aaaaatgatt tgcattcaaa attgagaagc gaaataatgc
     4321 ttcatcaaaa gcaaaatcat aatcgtcatc agataatgat tccattaatg tttgaatatg
     4381 ttttagtgag tagtcaggta tcgaatcggg ataatatact ggataatcag tggctgaaaa
     4441 attgttttct aatcctgaaa tagtaattat caaagattct atgtaatata ttaaagcatg
     4501 tttaatatta ccttcttgtt ccaaatgtct tccctgagcc tttcgtgtgt tagataagca
     4561 tccaaaatct cctgatacag tattcctatg agcttgttta tttaagagac tccattttat
     4621 atcaccaaaa gttgctttaa aagagatatt gtttttttct ttacaataat cttccatact
     4681 tatgtcttta tcatagtatg ctttgatata gtcactatgt tcgataataa gattttgtgc
     4741 ttcttttgtt agtttaaatt cgaacggaat atctaaattg tcaatgttaa cattattaat
     4801 tattcttgta attaattctg ctttttttcc agaagtagct aaattatgtt tagataatat
     4861 agtttttaat tctactactg taagatatga taaagtctta tttttagtga agtatcctaa
     4921 tttgtataat tgattgtgtg tagattcagg gtcaatttga agagagtatt taaaatatct
     4981 tgggaaatta taatttctcc ctgttttttg agtccaatct atgagtatga gttgcccagg
     5041 agtaaggcca cattctaatt cgtttaggta aaaattgtta caatcagaat ttgtgtctct
     5101 attatgatta ttttgtttta aattaaatag tttgctaaaa aaattcattg gattttattg
     5161 ctaatcaatt cttattgaaa aatgatactt ttacagtacc catgttcgga tggcatgggc
     5221 aacgccggat tcgtcatttg ttttggtgat gtatttggcg atttttttga tttctggatt
     5281 tccattttcc atgacaacgg ggtttccaac gacttccagc atgggacggt cattttcttc
     5341 atcaccgatt gccatggttt catctttggt caatccgagt ttttcagcca agtgagtaat
     5401 ggctgaaccc ttgtctacat tctttttaag gagttcgagg tagaaaggag cagatttgtt
     5461 gatggagtag cgctcgtaaa attctgctgg aattttttca atcgcagcat cgagaatttc
     5521 tggttcatcg ataaacatac atttaacaat ttctttgcca gccatttctt caggggtacg
     5581 gtagaagata ggcatgctga cgagggttga ttcgtgtaca gtgtattttc cgatattgcg
     5641 atttgcagta tagataccgt ccttggtaat ggcatgcatg tggacaccga gcttgcgact
     5701 gaggaattcc atatccagat aatcctcata ggtcaaggat tcgctgataa tctcatggcc
     5761 agtagcagtt tcttggacaa gggcaccatt gaaggtcacc acatagtcac cttggtctct
     5821 caactgcaag tcgtccagaa gtttggcaac acctgcgatg gggcggccag ttgcaatcac
     5881 gactttgaca ccagcttctt tggcatcttg gatggcagaa aagacttcag gagtgatttc
     5941 cttttggctg ttgactaggg ttccgtcaat atcgacggca attagtttaa tactcataaa
     6001 attcctcttc tagtttttat ttggggtaaa atgatcgttc tcaatcaagt gtaaaaattg
     6061 ctgggtaatg cttgcgaaga tgctgttttg gtctaacatt tctttaggga aatagaaacg
     6121 attatctccg tggcgactgc cagcaaggga ttggacgata ggagacaggc tagagagttc
     6181 ggccagttct ccattttttt gtaaaatctc aatctgtgtc cgtggatttt gagattcggg
     6241 acgatagata tcataaggga ggtcaaagtt cttatgaatg gcagtatagt agtcgggatc
     6301 aaagccgatg tcctcaacca attttctcat gctagtaagt tgatcttggt cctcttgtga
     6361 aaaggtaatg gatttaaaga ccttgcggtt gacaaagcgt tgagataaat ctgcaagaat
     6421 tttgtcagga ctagtcatcc aaagctggaa gtaggtattc atcacgccat catccagagc
     6481 cagatagtca gtcaaggtca catttttttc gaagaaaggc aggagatgtg gggaagttcg
     6541 tgcaaagaaa ttcttgtcct cagggtagag ttccttggcg cgcttgagaa gattctgtag
     6601 gagaacttcc atggcgcgtg ttgctgggtg gaaataaacc tgcatgtaca tctggtagcg
     6661 actgaggacg tagtcttcga tggcgtgcat gccattgcgc tgaaaggcga taccattttc
     6721 gacaggacga atgactcgga ggatgcgagt caggtcaaat tccccatagg atgctcctgt
     6781 aaaataggag tcgcgcaaga gatagtccat gcgatctgcg tcaatctgac tagaaatgag
     6841 ctgcacgacc tgcttattag gataggtatg gtcaatgaca ctggccacct tttctgggaa
     6901 atcaggtgcc acttgtagca ggacttggtg aatctctgtc tcaggatttt gaataatctc
     6961 ctgagtaatg gcttcatggt ctgtatcaaa gagattttca aaagtatggg agtaggcacc
     7021 atgcccaagg tcgtgtagga gagcagcggt cacggtcaag agagactcgg caggattcca
     7081 ttcctcagga tatttttctt cgaaaatctc tgtgatgcgt cgtgcaattt catagactcc
     7141 tagacagtga gagaagcgac tgtgttctcc accgtggaag gtataactgg aagttcccag
     7201 ttgtttgatc cggcgcaaac gctgaaattc ttttgtatta atcaagtcat agatgatttg
     7261 attattgaca tggatgtagt tgtgaactgg gtcacggaat actttttcgt tcatatgacc
     7321 attatagcaa aatcctgctc tttttggtaa aatagtagga caagagacaa gaaagagggc
     7381 ctgatgtgaa gattcggatg cgaaatacaa ttcagtttga tgagcagttg gaagtgattg
     7441 accagcttta tgatgtggaa gtgcatgaaa aaggagatta tagctacctg cttttctata
     7501 atgaggaaaa ggaaaaagta gtgattaaat ttcatggtca agaacttatg atgagccgat
     7561 tttctaatcc caagaccatt atgcgctttc taaaggatag cgatagttta gcctatattc
     7621 ctacacctat gggcatgcag gagtttatca tccaaacgag ccattatcaa gttgataggc
     7681 aaaagattga actagattat caactacaaa atcaagaggg acatcctttt gccagctatc
     7741 aattggaaat tacttggggc taggctcatg tctttttcaa atttagctat ctatcttctt
     7801 ggatcggctt cctagtgtgg tagattaggc taatttttgg tataataaaa gttatggaaa
     7861 tcgaaaaaac caatcgtatg aatgcgctct ttgaatttta tgcggcgctt ttgacagata
     7921 agcaaatgaa ttatatcgag ctctactacg ctgatgatta cagccttgct gaaattgccg
     7981 aggagtttgg tgtcagtcgt caggctgtct atgacaatat caagcgaaca gaaaagattc
     8041 tggaagatta tgagatgaaa ttgcacatgt actcggacta tattgtccgc agtcagattt
     8101 ttgatcagat tttggagcgc tatcccaagg atgactttct gcaggagcag atagaaattt
     8161 taacaagcat tgataataga gaataagagg aagaaaaatg gcatttgaaa gtttaacaga
     8221 acgtttgcag aacgtcttta aaaatctacg taaaaaagga aaaatctctg aatctgatgt
     8281 ccaagaggca accaaagaaa ttcgcttggc cttgctcgag gccgacgttg ccttgcctgt
     8341 tgtaaaggac tttatcaaga aagttcgtga gcgtgcagtc gggcatgagg tcattgatac
     8401 acttaatcct gcgcaacaga ttattaaaat cgttgatgag gaactgacag ccgttttagg
     8461 ttctgatacg gcagaaatta tcaagtcacc taagattcca accatcatca tgatggttgg
     8521 tttacaaggg gctggtaaaa caacctttgc tggtaaattg gccaacaaac tcaagaaaga
     8581 agaaaatgct cgtcctttga tgattgcggc ggatatttat cgtccagctg ccattgacca
     8641 gcttaagacc ttgggacaac agattgatgt gcctgtcttt gcacttggaa cagaagtacc
     8701 agctgttgag attgtacgtc aaggtttgga gcaagcccaa actaatcata acgactatgt
     8761 cttgattgat actgcgggtc gtttgcagat tgatgagctc ctcatgaatg agcttcgtga
     8821 tgtgaaagca ttggctcaac caaatgaaat cttgcttgtc gttgatgcta tgattggtca
     8881 ggaagcagcc aatgttgcgc gtgagtttaa tgctcagttg gaagtgactg gggtcatcct
     8941 taccaagatt gatggcgata ctcgtggtgg tgctgctctg tctgttcgtc acattactgg
     9001 aaaaccaatc aagttcactg gtacaggtga aaagattacg gacattgaaa ccttccaccc
     9061 agaccgcatg tctagccgta tccttggtat gggggatatg ctcactttga ttgagaaagc
     9121 ttctcaggaa tacgatgaac aaaaagccct tgaaatggct gagaagatgc gcgaaaacac
     9181 ctttgatttt aatgatttca tcgatcaatt agatcaggtg caaaatatgg ggccgatgga
     9241 agacttgctc aagatgattc caggtatggc aaacaatcca gcccttcaaa acatgaaggt
     9301 ggatgaacgc cagattgctc gtaaacgtgc cattgtgtct tcgatgacac ctgaagagcg
     9361 tgaaaaccca gatttgttaa atccaagccg tcgccgtcgt attgctgctg gttctggaaa
     9421 tacattcgtc gaagtcaata aattcatcaa ggactttaac caggctaaac agctcatgca
     9481 gggtgttatg tctggggata tgaataaaat gatgaagcaa atggggatta atccaaataa
     9541 ccttcctaaa aatatgccaa atatgggagg aatggatatg tctgcccttg aaggaatgat
     9601 gggacaaggc ggtatgcctg acttatcagc tctcggagga gcaggaatgc cagatatgag
     9661 ccagatgttt ggtggcggtt tgaaaggtaa aattggtgaa tttgctatga aacagtccat
     9721 gaaacgtatg gctaacaaaa tgaagaaagc gaagaagaaa cgcaagtaag acaagagatt
     9781 gatgcaaatc tcatttgatg agaaaataaa gtaaaaattt ctatgataaa acgtacagta
     9841 tcaaggtttt agaatatctg atattatacg ttttttgatt ttaataactt tgttaagaca
     9901 cttttattat ggtatacgaa aaatccactc agctgtctga gtggattagg tgtatttaga
     9961 ctcttagtct ttattttcgt atggcaagat caagttcaag atgattcctg tcatggctga
    10021 aagggcagta cctgaaagtg taactggacc aagtttaagg atagctcctc caagtccaag
    10081 aaccaacata gcacttgcga tgatgaggtt tcgcatttga gcgaaatcaa cacgttcttt
    10141 aatcaagact ttcaaaccat tgctggcgat aaccccatag agaaggattg acataccacc
    10201 aagtacagcg tttggaatag ttgaaatcaa ggcagtgaat ttaccaagga agctgagggc
    10261 aatcgcgatg aaggcagcgt tacggataac tgagacagaa gcgatacgag tcataccgat
    10321 aacccctgta ttttctccgt aagttgtatt ggctggtcca ccaaggaagg cggaaacaga
    10381 agttgcgata ccgtcaccaa taagagtacg gtgaagacct ggttctttta agaattgacg
    10441 gccacagatt tgacccaaaa cagtatggtc tccgatatgt tcagaaattg ttacgatagc
    10501 gattggcaag atagcgatgg cttctggacc aaagtaaaga ttgtactctt taaaggcacc
    10561 acctgtgcta aatggcaagt agaaaccagg aatttcaaac cagttagctt ctaggacagg
    10621 cgtaaagtca accaaaccaa gagttagtgc gaagaggtaa ccaccgataa tggcaaagag
    10681 gaatggaatg attcgtagga agccttttcc ttttgtattg ataaaggcag caattaggaa
    10741 agtaacaacg gctaccagag catttttcca atttccgtct gctacaagac ctgcattggt
    10801 aacagctgaa cctgcaagtc caagaccgat aacgatgatc ataggaccga taatgattgg
    10861 tggcaagagt ttatcaatcc attttgttcc tacaaatcgg atgctggtag caacaaggac
    10921 atagaccaaa ccagtcaaga taacccctgt ttgggcagca gatacatccc cccccatttc
    10981 tttcatagcc agtgacatag ctgtgataaa ggcgaatgaa gaacccagat aaactggaac
    11041 tttaaaacca gtagcaatca tgtagatgag tgttccaaca cctgaagcaa aaagggcaac
    11101 agatacaggc attcccaaaa tcaatggtac caagatggtc gcaccaaaca tggcgaaaac
    11161 gtgttggaaa ctaaggagaa ttccttttcc agccgaagga cgttggtcaa cgtctagtaa
    11221 caaatcaaca gttgattctt gtttcatata aacctcactt ttgggcatca aaaaagagcc
    11281 cattatttta cagcaatagg ccctcagagt aagacttctt taaaatttag attttaaaga
    11341 gttttaaata tttctgcgtc ttcgtcacct cacgggatga cattaaaaac ctttgcttag
    11401 gatagtatag cagaaaaaaa tgattttgta aatcattttt tcccgagcct agaaataaaa
    11461 gagcgaggct gattttgtaa acatttgttt actaaaaaat ttaaagtggg cgtttatttg
    11521 gcataccagc cttacgtgga tatttatttg gtgtttcttt tttcttttct accactgtga
    11581 tatagcgcgg atctctattc ggtagggcgt agctgagatt gtcttcgacc ttactaaaaa
    11641 ggagattgag ggcattctta gcttctaata attcctcagg cgcattgctg gccttgagtg
    11701 ccaatagttt gccaccaacc ttaaggtagg gaatagtcaa ttcagatagg acctgcatac
    11761 gggcaaccgc acgagctgtt acaaaatcat attgagcacg gaagttcttg tcttgggcaa
    11821 aatcttcggc acgtccgtgg tagaaatgaa ctccgttcaa atccagttct tgagccaaga
    11881 gttgtaggaa gttgatgcgc ttattgagtg aatcaataat ggtcacatct aactccggat
    11941 agagaatttt cattggtaga ctaggaaatc ctgccccagc cccgatatca agaagtttga
    12001 tagtttcatt gggaatcaaa ccttgaagaa tgggtgcaat cgaatcgtaa aaatgtttga
    12061 gataaacttc ttccttgtcc gtaatcgccg tcaaattaat cttctcattc cactcgacca
    12121 agagctcaaa ataacgttca aattgttctt tttgctggtc cgaaagtgga agattctgct
    12181 cggcaagcaa gttgtaaaat gtttctggtt tcatagttat ttccttcttt agtatcatct
    12241 tattttacca caatcataga aaatttgata tactggtact aatctaaaag cagaaaggac
    12301 ttatattatg acttggatta ttcttggagt tttagctctt gttgttattt ttgtaattgt
    12361 tagctataac ggtttggtta aaaatcgtat gcaaaccaag gaggcttgga gtcagattga
    12421 tgttcagttg aaacgtcgca atgatctctt gccaaacttg attgagactg taaaaggtta
    12481 tgccaaatat gaaggttcta cccttgaaaa ggtggcagaa ctacgtaacc aagtggcggc
    12541 agcgacttca ccagcagaag ctatgaaagc cagtgatgcc ctcactcgtc aggtttcagg
    12601 tatttttgca gttgcagaaa gctatccaga tttgaaagct agtgctaact ttgttaaatt
    12661 gcaagaggag ttgacaaaca cagaaaataa aatttcttac tctcgtcaac tctataacag
    12721 tgttgtcagc aactacaatg taaaattaga aactttcccg agcaatatta tcgctggaat
    12781 gtttggattt aaagcggcag atttccttca aacatctgaa gaggaaaagt cggttcctaa
    12841 agttgatttt agcggtttag gtgactaaga tgttgtttga tcaaattgca agcaataaac
    12901 gaaaaacctg gattttgttg ctggtatttt tcctactctt agctcttgtt ggttatgcgg
    12961 ttggttatct ctttataaga tctggacttg gtggtttggt tatcgcactg attatcggct
    13021 ttatctacgc tttgtctatg atttttcaat cgacagagat tgtcatgtcc atgaatggag
    13081 cgcgtgaggt ggatgagcaa acggcaccag acctctacca tgtagtggaa gatatggctc
    13141 tggtcgctca gattcctatg ccccgtgttt tcatcattga tgatccagcc ttaaatgcct
    13201 ttgcgacagg ttctaatcct caaaatgcgg ctgttgctgc gacttcaggt ctactagcta
    13261 tcatgaatcg tgaagaacta gaagctgtta tgggacatga agtcagtcat attcgtaatt
    13321 atgatatccg tatttcgact attgcagttg cccttgctag tgctatcacc atgctttcta
    13381 gtatggcagg tcgtatgatg tggtggggtg gagcaggtcg cagacgaagt gatgatgacc
    13441 gagatggaaa tggtcttgaa atcattatgc tagtggtttc cctactagct attgtactgg
    13501 cacctctcgc tgcaaccttg gttcagctcg ctatttctcg tcagagggaa tttctggcag
    13561 atgcatctag tgtcgagctg actcgcaatc cccagggaat gattaatgcc ctagataagt
    13621 tggacaatag caaacctatg agtcgccacg tcgatgatgc tagcagtgcc ctttatatca
    13681 atgatcctaa gaaaggtggg gggttccaaa aactctttta tacccaccca cctatctcag
    13741 aacggattga acgtttaaaa cagatgtaaa agaatccaga ggtcgtctct ggatttttgc
    13801 tatgttgcaa atttataaat cttctaaatt aactagcttc aaaccgtgtt ctgtcatgcg
    13861 atagatgatc gagcagactt cttttaagaa acgacggtca tgcgaaacag tgatgagacc
    13921 gcctggatag gttgcaaaga gttttctgat ttggggttga gaagtgggag aaaagtttcg
    13981 tgtgggttca tccagcagga gaaagtttgg tttgcgcaga actagatcca aaagcaggag
    14041 ttttccctgt tgtccgccag ataaggagcg aatttgatgc tgcatttctg gataactgaa
    14101 attgagactg gctaggtgag attggatttt ctgaagttcc tctttttccc cagttttgct
    14161 gatataagct attgaggata aatccaattg cagttttttg tggtaatctt gtggcataaa
    14221 accaagtgaa atctctcttt tatcattaag aagttgctgt aatttggcta acagagttga
    14281 tttcccaaca ccatttgggc cgatgatacc gattttttct tggccacgga cagttagttg
    14341 cagttcctga gccaaaacac ggtcgtcaat ggacaaattt tctttttcca gttggactaa
    14401 gactttagaa gctggtaatg gttggatgtc tgaaaagaaa agttggattt gttcctcttc
    14461 aagtggcttt tgagtcatgg actgagctgt cttttcgtag cgtttttctt gtgagaggac
    14521 agttttcatc tttttagcca ataggcgacc ggcagtacta tctttggtag ctcgaagcgc
    14581 agtttctaca ttttgcttaa ctctccgatg tttttccatg gttttatcgt aggctctttg
    14641 gttgttagca gcttgctgac tttgtttggc aaaattagcc tttctctgct cactatagct
    14701 atcatagtct aaatgctcta ctagcgtttc cgcttcttta cggtgtttga ccagtcgcaa
    14761 gtgaacaata gtgtctgccg tttcagaaag aaagtcttca tcatgggaaa tgaaaataac
    14821 ggtttgcctg gtcttttgaa tctggccttt tagccaatca actgtctcaa ggtctaggtc
    14881 atttgaaggt tcatctaaaa atagaatctc aaagggtttg gctaactcat ggataagctg
    14941 aattttcaaa gcttcgcccc ctgatagatt gccaatctct tggtcacttg cgaaacgatt
    15001 gctatcaaaa tgcaattcct ccgccaaacg atagaggata ctgtagtcta aatcaataga
    15061 atctaaaaag aagtagtcgt gtaaagtttt cttttttagg tcctcaggga ctttttgagg
    15121 aatgtaggcc agtgactgat aatcagactg gatgtctccc ttgatagtga aatcagacaa
    15181 atcttccccc attaaagttt taagtaaggt tgatttacca tttccttctt cgccaataat
    15241 agctaccttt tccccgtctt gtatggtcat gcttaggtca gagacaaggt ctcgtaaatc
    15301 tttgttttgt gtgatggtca ggtgattgat ttttatcata ctattgccct ttctagaatg
    15361 tcgatagtgc ataacaaaaa gctctacttt acctagtaga gcccaaagtc tatgtcaaaa
    15421 aaactctatt aacatgccag aaaaagagca taaaagagca cacaaaaaga gacaaaaaca
    15481 agatctacta aataaaggtt ctttatttga tagacttagt tgttcatgtc tcccttacct
    15541 ccgagtatta gtatctttat actatacttt taaggaaatt ttgtcaacag aaaactggaa
    15601 attctatgct ctaaaatcca aaaacaggtc catagagact tagtacgtgt ttgacatgct
    15661 cgtaatggat tttatcatag tttcgccaag cagtgcgtgc ttgatcgttt agttttaggc
    15721 tacccagtcc aatcagagga ctggtgggtt ggtaaaattt ctcaagatca attaagatac
    15781 tgttgttcaa cttttccgtt tttttgagct cttgcatagt agtgtttagc agctcttgta
    15841 gtcgaatatc atctgccgta ccctgtttac agttttggta gcttttattt aacttggata
    15901 ggagttgaat gagtttcttg tcttccatgt cttccatatg agcatcctcc ttgatctgat
    15961 gtactctttc cctatagtat agcactaaaa aataaaactg tcatagtaaa tatgttaaaa
    16021 ttttgaattt taattacaaa gtttggtgtg ctgatttgta gccttttcat ggtataatca
    16081 agagagtaaa tctttatgga ggaagtatga aattaaatat tcaagaaatt cgtaagcagt
    16141 ctgaaggttt gaactttgaa caaacgttag atttagttga tgacctgcgt gcacgtaatc
    16201 aagaaatttt agatgtaaaa gatatccttg cagttgggaa agtacaatat gaagaccgta
    16261 tgtatttctt agattatcaa ctatcttata ccattgttct tgcttcgagt cgcagtatgg
    16321 agccagttga gttagttgaa tcttatccag tcacggaagt tttcatggaa ggcgcaacta
    16381 accagctaga tcaagaagtt ttagatgatg acttggtctt gcccattgaa aatggggagc
    16441 ttgaccttgc tgagagtgta tcagacaata tcctgctaaa cattcctatc aaggtcttga
    16501 cggctgaaga agaagctggt caaggattta tctcaggaaa tgactggcaa atcatgacag
    16561 aggaagaata ccaagctcaa caagcagtaa agaaagaaga aaacagtcct tttgctggct
    16621 tacaaggact atttgacgga gatgaataat ggtcttgtca aaaaaacgag cacgaaaggt
    16681 gctagaagaa atcattgccc tcttcccaga tgccaagcct agtcttgatt ttaccaatca
    16741 ttttgaactc ctggttgcgg tcatgttatc agcccagacg acagatgcag cggtaaataa
    16801 ggccacacca ggtctctttg ttgcctttcc aacaccccaa gctatgtctg tagcgacaga
    16861 gagtgagatt gcttcacata tttctcgctt gggattgtat cggaataaag ctaaattcct
    16921 taaaaaatgt gcccaacagt tactagacga ttttgatggt caagtccctc agacacgtga
    16981 agaattggag agtttggcag gtgttggtcg caagacagcc aatgttgtca tgagtgtagg
    17041 atttgggatt ccagcctttg cagtggatac tcatgtggag cgtatttgca aacaccacga
    17101 tattgtcaaa aaatcagcga cgccacttga ggtggaaaag cgggtcatgg atatcttgcc
    17161 gcctgagcag tggttagctg cccatcaggc catgatttat tttggaagag ccatttgcca
    17221 tccaaaaaat ccagagtgtg accagtaccc acaattatat gattttagca atttgtaaga
    17281 tgctacattg aaactttttg cttgatttta agcatgcttt gtgctatagt aaatgataac
    17341 taaatattcc tttaaacctg tcccgtgagg caggcaagga gcgcgataaa gtagaagggt
    17401 gaagtctata ctgtttgcag tagggctcgc ttggctataa atttcaagtc tccttgttta
    17461 aggagacttt tctttttgga ggtttgtcat gaaaacaaaa gaagttgtag acgaattgac
    17521 cgtcaaacga gcgattacgc gtattactta tgagattatc gaacgcaaca aagatttgaa
    17581 taagatcgtc ttggctggta ttaaaactcg tggtgtcttt attgcccacc gaatccaaga
    17641 acgtttgaag cagttagaaa atctttcagt tcctgttgtg gaattggata ctaaaccttt
    17701 ccgtgatgat gttaaaagtg gagaagatac ttctttggtt tctgtcgatg tgacagaccg
    17761 cgaagttatc ttggtggatg atgtgctcta tacaggtcgt accatccgtg ctgctattga
    17821 taatattgtc ggtcatggtc gtcctgcgcg tgtgagttta gcagttctag tcgatcgtgg
    17881 acatagagaa ttgccaatcc gtccagatta cgttggaaaa aatatcccaa ccagtcgttc
    17941 tgaagaaatc atcgtagaga tgacagaact tgatgaccaa gacagagttc tgattactga
    18001 agaagcttag aaagttaaag gagtagccat gtcagaaaat caacaagcat tgaaccatgt
    18061 ggtgtccatg gaagacctca ctgtcgatca agtaatgaaa ttgatcaagc gaggaattga
    18121 gtttaaaaat ggagcccagc ttccctatga agaccatccg attgtttcca atctcttctt
    18181 tgaggattct acacggacac ataagtcctt tgaagtcgca gagattaaac ttggattgga
    18241 acgacttgac tttgatgtga agactagttc ggttaataag ggtgagacac tttatgacac
    18301 cattttgact ctgtctgctt taggagtgga tgtctgtgtg attcgccatc cagaggtcga
    18361 ctactataga gaattgattg ctagtccaac gattacgact tccatcatca atggtggaga
    18421 tggttcgggc caacacccta gccagagctt gcttgatttg atgaccattt atgaggaatt
    18481 tggccacttt gagggtctta aagttgctat tgcaggtgac ttggaccact cacgcgttgc
    18541 caaatccaat atgcagattt tgaaacgctt gggagctgaa ctctttttcg ctggacctga
    18601 ggaatggaga agtcaagagt ttgcagacta tggaaagttt gtaaccattg atgaaatcat
    18661 tgatcaggtt gatgtcatga tgtttctccg tgtgcaacac gaacgccatg atagtggagc
    18721 tgtattttca aaagaagact atcatgccca acatggcttg actcaagaac gttacgatcg
    18781 tttgaaagaa acagcaatcc tcatgcaccc agctccaatc aatcgtgatg tagaaatagc
    18841 agaccacttg gttgaagcac caaaatcacg gattgtccaa caaatgacca atggtgtctt
    18901 tgttcgaatg gcaatcttag aatccgtact agcgagtaga aacgccaact aaaacacaag
    18961 acgttaaaag acgccagtga gcgtccacga gaggtgaaaa tatgacaaaa agaattctag
    19021 tattagaaga tggcacagtt tttgaaggca aggccttcgg agcagatatt gatgtaacag
    19081 gcgaaatcgt ctttaataca gggatgaccg gctaccaaga atccattaca gaccagtctt
    19141 ataatggaca aatcttgacc tttacttatc ctttggtagg aaattatggt attaatcgtg
    19201 atgattatga atccattatt ccaacttgta agggagtcgt tgtttttgaa gaagcgcgta
    19261 gagctagcaa ctggcgcaac caaatgacct tggatgaatt tttgaaagcc aagaaaattc
    19321 cagggatttc aggaattgat acgcgtgctc ttaccaagat tatccgtaag catggtacta
    19381 tgcgtgcaac cttgacccat gttggggaca gtatggacca tgtgacggac cagctccaag
    19441 caacagtttt accgacagac aatatcaaac aggtttctac taaaacgtct tatccagctc
    19501 caggagttgg tttgagcgtg gtgctagtgg actttggtct caagcactca atcctacgtg
    19561 aactttctaa acgcaactgt aacgtgacag ttgttcctta ttcgacaacg gctgaagaaa
    19621 ttctccatct caatcctgat ggagttatgt tgtcaaatgg tccaggtaac ccagaagacg
    19681 ttccacaagc actcgacatg attcgcggtg tacaaggaaa aattccaatc tttggtattt
    19741 gtatgggaca ccaactcttt gcaatggcaa acggggctaa gacctataag atgaagtttg
    19801 gccaccgtgg attcaaccat gcggtacgtg aaatcgcaac aggacgagta gacttcacca
    19861 gccagaacca tggttatgca gtcagccgcg aggatttgcc agagcacttg attattaccc
    19921 acgaagaaat caatgacaag tcagtggaag gtgtgcgcca cagataccaa cctgctttct
    19981 ctgttcaata ccacccagat gcagctcctg ggccacacga cgctagctac ctatttgacg
    20041 agtttatcga gatgatggaa gtttttaaac aatcaaacta agaatgagta aaagctcgtc
    20101 ggagaattta tgtaactgaa catggacgga aagctcagaa tttagagaaa ccaacttgga
    20161 ttgtcaatcc ttccttgatt tcctaaaatt caatcgcttt ctagtcgtcc tttgtatctt
    20221 atttaatgtc gccctgagag gcgacgaata gaaaggagaa gatacattgt tcgcggaagt
    20281 gaacacgccc taagaactgt gtgaaaaaga taaacatcgt cttgacgctg aaggcgtctg
    20341 cgccgagttt cctattttca ctgtgttctt tacgggcttt gtatcataaa ctatgcctaa
    20401 acgtactgat attcaaaaaa ttatggtgat tggttctggt ccgattatta ttggtcaggc
    20461 tgctgagttt gactacgctg ggactcaggc ttgcttgtcg ttgaaagagg aaggttatga
    20521 ggttgttttg gttaactcaa atcctgcaac catcatgacg gacaaggaaa ttgctgacaa
    20581 ggtttacatt gaaccgatta cacttgagtt tgtgacacgt attcttcgta aggaacgtcc
    20641 agatgccttg ctaccaacac tcggtggtca gacaggtctc aacatggcca tggaattgtc
    20701 taaaaatggt atcttagatg agcttggcgt tgaacttctg ggtaccaaat tatctgccat
    20761 tgaccaagcg gaggaccgtg acctctttaa acaattgatg gaagagcttg aacaacccat
    20821 tccagaatct gaaattgtta acacagtaga agaagcagtt gcctttgcag cgacaattgg
    20881 ctacccagtc atcgttcgtc cagcctttac cctcggtggt actggtggtg gtatgtgtgc
    20941 caacgagaaa gaattgcgtg aaatcactga aaatggtttg aaattgtcac ctgttaccca
    21001 atgtttgatt gagcgttcga ttgccggttt caaggaaatt gaatacgaag tgatgcgtga
    21061 ctcagctgac aatgccttgg ttgtttgtaa catggaaaac tttgacccag ttgggattca
    21121 cacgggggat tccattgtat ttgcccctgc gcaaaccatg tcagactatg aaaaccaaat
    21181 gcttcgtgat gcgagcttga gcattattcg tgccctcaag attgaaggtg gatgtaatgt
    21241 tcagctagct cttgatccta acagcttcaa gtattatgtt atcgaagtaa accctcgtgt
    21301 atcacgttcg tcagcccttg cttctaaagc gacaggatat ccgattgcta agttggctgc
    21361 caagattgca gtaggtttga ccttggatga ggttataaac ccagttacag gttcaaccta
    21421 tgccatgttt gaaccagccc ttgactacgt agttgctaag attccacgtt tcccatttga
    21481 caagtttgaa aagggtgaac gccgtctcgg tacacaaatg aaggcgactg gagaagtcat
    21541 ggcaatcggt cgtaacatcg aggaatctct tctcaaggcc tgccgctccc ttgaaattgg
    21601 ggtgcaccac aatgaaatac ctgaacttgc agcagtttct gatgatgcct tgattgaaaa
    21661 ggttgtcaaa gcccaagatg accgtctctt ctacgtatca gaagcaattc gtcgtggcta
    21721 cacacctgaa gaaattgcag agcttacaaa aattgatatc ttctatcttg ataaactctt
    21781 gcatatcttt gaaattgagc aagaattggg tgctcatcca caagatctag aagttttgaa
    21841 aacagccaaa cttaatggtt tttcagaccg taagattgct gaactctggg gaacgactga
    21901 tgatcaagtt cgccaacttc gcttggaaaa caagattgtt ccagtttata agatggtaga
    21961 cacttgtgcg gcagagttcg actccgaaac gccatatttc tattcaacct atggttggga
    22021 aaatgaatct atcagatctg ataaagaatc tgtacttgtc ctaggttcag gtccaatccg
    22081 tattggtcaa ggggttgagt ttgactacgc aaccgttcac tctgttaagg ctatccaggc
    22141 agctggttat gaagctatca tcatgaactc aaacccagag accgtttcaa cagacttctc
    22201 tgtatcagat aagctttact ttgagccatt gacattcgaa gatgttatga acgtcattga
    22261 cttggaacaa ccaaagggag ttatcgttca gttcggtggt caaacagcca tcaaccttgc
    22321 ggagccattg gcaaaagcag gtgtgaccat ccttggtaca caggttgctg acctagatcg
    22381 tgccgaagac cgtgacctct tcgagcaagc tctcaaagaa ttggatattc cacagccacc
    22441 aggacaaaca gctaccaatg aagaagaagc agctcttgca gctcgcaaga ttggcttccc
    22501 agttcttgtc cgcccatctt atgtacttgg tggacgtgcc atggaaatcg ttgaaaacga
    22561 agaagacctt cgttcttaca tgcgaactgc tgttaaggct agtccagacc acccagttct
    22621 tgtcgactct tatatcgttg ggcaagagtg cgaagttgat gccatttcag atggtaaaaa
    22681 tgtactcatt cctggtatca tggagcatat cgaacgtgct ggtgtccact caggtgactc
    22741 aatggctgtt tacccaccac aaaccttgtc gcaaaaggtt caggagacca tcgcagacta
    22801 caccaaacgc ttagcaatcg gtcttcactg ccttggaatg atgaacatcc agtttgttat
    22861 caaggatgaa aaagtctacg ttattgaggt caatccacgt gccagccgta ctgttccatt
    22921 cctatctaag gtaaccaata ttcctatggc tcaagtagcg accaaactca ttcttggtca
    22981 aagtttatca gaacttggct accaaaatgg actttaccct gaaagcaccc gtgttcatat
    23041 caaggcacct gtcttctcct ttaccaaact agctaaggta gacagcttgc tcggtcctga
    23101 aatgaagtca acaggtgaag ttatgggttc tgatgctact ttggaaaaag ctctctataa
    23161 agcctttgaa gcttcttatc tacacttgcc aacttttggt aatgtcgtct ttaccatcgc
    23221 tgatgatgcc aaagaagaag ccttgaactt ggctcgtcgt ttccaaaaca ttggttatgg
    23281 aatccttgcg acagaaggga cagcagcctt ctttgctagc catggcttgc aagcccaacc
    23341 tgttggtaag attggtgatg acgataagga tatcccaagt tttgtacgca aaggaagaat
    23401 tcaagctatc attaacacag taggaacaaa acgaactgct gacgaagatg gtgagcaaat
    23461 tcgtcgttca gccattgaac acggggtgcc cctcttcaca gcgctagata cagctaatgc
    23521 catgcttaag gtacttgaaa gtcgtagctt tgttacagaa gcgatttaaa tagaaaaata
    23581 agatatagca gactgaattt agaacagtac acaggaatac taaaatattt ctagaaatta
    23641 atttgaattt tctaattgat ttgttcgcat cttatttcaa tctactatag aaaaaagtct
    23701 ttaaaattat aaaacgcatc atatcaaggt ttttcaaaaa ccttgatatg atgcgtttta
    23761 ttgtgggaat atgtatttca ttttctacta aaattatgtt tttgaataac ctctatctta
    23821 gtagtttgta taatccccct caatcagttt ttgcgataag ctttaatgct atgactatac
    23881 cactcttgca tttcttttga tggtggtgtc atataatcgc catacatctg ggttaaaaat
    23941 tggtcatatt ttttgggaac aggcaacata cggccctcaa actcagttaa aatcagttct
    24001 ttaaaggtat caactgggaa gatttctttc atcccttcct taccgatccc aactcctcct
    24061 tcatattgag gaatgttggt tacagcattt ttgactagtt gatcaatttt cttgtaaaag
    24121 tagcgaggat tgacaaatcg gagagcgtac cagctacata atctaagaaa atcttttagt
    24181 ttgctatcac cgtgaactgc tcgtgatttt ttgatgtaag ctagttgacg aagggccaca
    24241 tacttatagc tcttgtcgac aatgctcaag tctgtaaatt gatcaattgg gaagacatcg
    24301 atgaaaaggc tggtatcatg acgcttgtac ttaacatggt cttctataac agtagaagta
    24361 tccaaaatcg atgcgaaatt atggaagtac caagaagatg tatcgtagga aagaaccttg
    24421 tagcgagggt gattttcttc ttcaataatc ttcagtaaac gctcataatc ctcacgataa
    24481 agggaaatat caatatcatc atcccaagga atcatacctt tgtggcggat ggctccaagc
    24541 atggttccat aactgagaaa ataaggaata tcatgtttct tacaagtctc atcaatatag
    24601 tccagcaggg ctagttgaat ttctttaatt tctttttttt ctaaatattg catcctaatc
    24661 ctccaattta taagcgtgaa attcatgact gtagaagcgt ttttcttctg gtggtagggt
    24721 catataatct ccataaaatt gtgtcaaaat agtatcaaat ttttcaggtg caggaaggct
    24781 taaattctca aagggtaaat cgattgtttt atcaaaggta gcacttggga agacttcctt
    24841 ttccttaaat tttgaaggaa taaaagccat atactgccca ttttcacgac tatatttttg
    24901 aatttctttc tcgattttat ttgcaaaata acgaggagaa accagtcgaa ggagcaacca
    24961 aaaggctgtt cgtatccaat cttttaaaag gctatcctta tagacaatat ttttatgttt
    25021 actgaaagac agcagtttga agctttctag tttataacaa gtatcaatga ccttaggatc
    25081 atcaaagcga tccataggga aaatatcgat aaagatacca gactcatagg tttttgtatt
    25141 tcgagtatca ataattttag tcgtactgtc ggttatcttg ataaagttgt taaagtagtt
    25201 cttatcagtt tctaaggata ggagcttata cttgcttttt tccttttgaa aaatgttaat
    25261 aaatcgttgg tagtcttctc taggcatgga taaatcaata tcgtcgtccc aagggataaa
    25321 gccctcatgt cgaaccgccc caatcagagt accgtagtta ataatatagt tgatattgtg
    25381 ctttttacag agagtatcaa tataattcaa aatttctaat tcaatttgtt tggcatcttc
    25441 aacggttagt tgtttcattt taaactccta agatttttta aatttatttt ttaaggctag
    25501 gacatggttt aaaaattcat agaaaatgct atcttttgtg aagacaagta gactaatata
    25561 agagatagct gatagcaaga caatcaaacc agtattaatc aaaaatggca aattaatgac
    25621 catatccaca ggatacacga aattaatcag gaaataaatt gctacaaagg aaagtgaaaa
    25681 gagagagtat cgaacagtat agctaaagat atgtcccaag tggatgagtt gttttctatg
    25741 gatgaaaatg atatagaaaa caagtagaga agcctctgat aacatagttg tcagtaagta
    25801 gtattcggga gccacgatat ggttgaaaaa gaggagacta ttcaagccca aattgagtag
    25861 cccagcaaag actgtataga ctgtgatacg tttttcatag ccattggtaa agagaatttg
    25921 ggaaccaaga atggtatcta aggccaggat aatcgtacga aaagcgaaga gagaggtcaa
    25981 gatgtcgcct ccgatatatt tttcactacc gtaaagtagg atggcatttg gtcctaaaac
    26041 catgagtcca aaactcagtg gaatgataaa gaagttaaag attcgactac ctctattaac
    26101 cagagaaaca taggcttctt tgtctccttt ccccagatag taactgagac gaggcacact
    26161 cactccaatt gcacctgtta caaccccagc tataacggtc acaattcgct gagctatggt
    26221 atagtaacta acgttgacat caatccctgt tttaacgagg aagaggcgat ctaaaaaagt
    26281 gaagagcata ttggcattgg caaagactaa catggctgtc agagggagaa agagtggttt
    26341 aaaatcactt aggtgaatct taacaagttt gatgtctctt ttaatccaaa aataactaat
    26401 caggtagtta atcagcgtcg ataaactcat cacaagtgta tagacaacaa tatcgtgttc
    26461 atttttaaca aataagaaaa tagagaccag catcaggata cggatgaagg cagttttgta
    26521 aaagagaaaa ctgtaatttt ccagagcttc attgacccat tcgattgaaa aaatctgggc
    26581 aatgagttga atccccataa caaggtagac ctttttgacg attggattat cagtaaagaa
    26641 gagaggatag gctaggatat agacagcagt ggtcaaaatc gtacaagcga tgcacaaata
    26701 aaaaagacta gaaaaggttc tgttaagatc ttttttgtta tccttgacat tactgatagc
    26761 ccttaaaccg tagttataga caccataagt tgcaaagggc aagaaaaatg acaaaatagt
    26821 gtcgactgag ttgaagtaac catagtcagt tcggtccaag acacgcgcga cataggttcc
    26881 agttaggatg ggaaaaataa tattcaagac acgaattccc atgtaagata gagcatttaa
    26941 ttttatactt ttcattcaat ttacctcgtt tttcattata tcataaagtt agctaataag
    27001 aaatgaaggg cagtaagtca agtaatcact ttgaagtttc aaatcttaag ttttaagttt
    27061 tctttaagga aagtatatta ttctgaagga ctctaaaatt tcgcagccat ttattagtaa
    27121 ttgctacaga attcctagtc attactagaa atggactagt ttctttgaat aatagaactg
    27181 cataattctc ctattctaga aggggaggac cagtatttct tttatgatag gactagattg
    27241 tggtataata gagagaataa gtttttttag taagacaaag gagaaaatag atgatttatg
    27301 caggaattct tgccggtgga actggcacac gcatggggat cagtaacttg ccaaaacaat
    27361 ttttagagct aggtgatcga cctattttga ttcatacaat tgaaaaattt gtcttggagc
    27421 caagtattga aaaaattgta gttggtgttc atggagactg ggtttctcat gcagaagatc
    27481 ttgtagataa atatcttcct ctttataagg aacgtatcat cattacaaag ggtggtgctg
    27541 accgcaatac aagtattaag aaaatcattg aagccattga tgcttatcgt ccgcttactc
    27601 cagaggatat cgttgttacc cacgattctg ttcgtccatt tattacactt cgcatgattc
    27661 aggacagtat ccaacttgcc caaaatcatg acgcagtgga cacagtggta gaagcggttg
    27721 atactatcgt tgaaagtacc aatggtcaat ttattacaga tattccaaat cgtgctcacc
    27781 tttatcaagg acaaacacct caaacattcc gttgcaagga cttcatggac ctttatggat
    27841 ctctttctga tgaagagaag gaaatcttga cagatgcatg taaaatcttt gtgatcaaag
    27901 gaaaagatgt ggccttggcc aaaggtgaat actcaaatct gaagattaca accgtaacag
    27961 atttgaagat tgcaaaaagt atgattgaga aagactagta aaatgattaa tcaaatttat
    28021 caactaacta agcctaagtt tatcaatgtc aaatatcagg aagaggctat tgaccaagag
    28081 aatcatatcc ttatccgtcc caactacatg gctgtctgtc atgcggatca gcgttactat
    28141 cagggaaaac gtgatcccaa gattttgaat aaaaagcttc caatggcaat gattcacgag
    28201 tcatgtggaa ccgtcatttc tgacccgacc ggaacctacg aggttggtca aaaagttgtc
    28261 atgattccca atcagtctcc tatgcagagt gatgaagaat tctatgaaaa ctacatgaca
    28321 gggacccatt tcttgtctag tggatttgat ggctttatga gagagtttgt ttctctccct
    28381 aaagatcgtg tggtggctta tgatgctatt gaagatacgg ttgcagccat tacagagttt
    28441 gtcagtgtgg gcatgcacgc tatgaatcgt ctattgactc ttgctcatag caagcgggag
    28501 cggatcgccg ttattggaga tggaagttta gcttttgtgg ttgccaatat tatcaactat
    28561 actttgccag aagcagagat tgtggttatt ggtcgtcatt gggaaaagtt ggaactcttc
    28621 tcatttgcca aagaatgcta tattacggat aatattcctg aagagttggc ctttgaccat
    28681 gcttttgaat gttgtggtgg tgatggtact ggaccagcta ttaatgactt gattcgctac
    28741 attcgtcctc agggaacgat tctcatgatg ggagttagcg aatataaagt caatctcaat
    28801 actcgcgatg ccttagaaaa gggcttgatt ttggttgggt catctcgttc tggtcgcatt
    28861 gattttaaaa atgctatcca aatgatggaa gtcaagaaat ttgccaatcg tcttaaaaat
    28921 atcctttatc tagaagaacc tgtaagagaa attaaagata ttcatcgtgt ctttgcaacc
    28981 gatttaaaca cagcctttaa aacagtgttt aagtgggaag tataagtact ggaggttaat
    29041 tgtggagaaa atcattaaag aaaaaatttc ttccttactt agtcaagaag aggaagtcct
    29101 cagtgttgaa caactgggtg gaatgaccaa tcaaaactat ttggccaaaa caacaaataa
    29161 gcaatacatt gttaaattct ttggtaaagg gacagaaaag cttatcaatc gacaagatga
    29221 aaagtacaat cttgaactac taaaggattt aggcttagat gtaaaaaatt atctttttga
    29281 tattgaagct ggtatcaaag taaatgagta tatcgaatct gcgattacgc ttgattcaac
    29341 gtcaatcaag accaagttcg acaaaattgc tccaatatta caaactattc atacgtctgc
    29401 taaggaatta agaggagaat ttgctccttt tgaagaaatc aaaaaatacg aatccttgat
    29461 tgaagaacaa attccttatg ccaactatga atctgttaga aatgcagtct tctccttaga
    29521 gaaaagactg gctgacttag gtgttgacag aaaatcttgt catatcgatt tggtgcctga
    29581 aaactttatc gaatcacctc aaggacgact ttatttgatt gactgggaat attcatcaat
    29641 gaatgatcca atgtgggatt tggctgccct ctttttagag tctgaattca cttcccaaga
    29701 ggaagaaact ttcttatctc actatgagag tgaccaaaca ccggtttctc atgaaaagat
    29761 tgctatttat aaaattttac aagatactat ttggagtcta tggactgtct ataaggaaga
    29821 gcaaggtgaa gattttggtg actatggtgt gaatcgttac caaagagctg ttaaaggttt
    29881 ggcttcttat ggaggttcag atgaaaagta aaaacggagt tccttttggc cttctctcag
    29941 gtattttctg gggcttgggt ctaacggtta gtgcttatat cttttcgatt tttacagatt
    30001 tgtcaccctt tgtggtggct gcagctcatg attttttgag catctttatc ttactagctt
    30061 ttctcttggt aaaagaaggg aaagttcgcc tctcaatttt cttaaatatt cgcaatgtca
    30121 gtgttatcat cggagccttg ctagcaggcc ctatcggtat gcaggccaat ctttatgcag
    30181 ttaagtatat cggaagttct ttagcttcat ctgtatcggc tatttaccct gcgatttcag
    30241 ttctattggc tttcttcttt ttgaagcaca agatttcgaa aaatactgta tttgggattg
    30301 tcttgattat tggagggatt attgcccaga cctataaagt tgaacaggtt aattctttct
    30361 acattgggat tctttgtgct ttggtttgtg ctattgcatg gggaagtgag agtgttctta
    30421 gctcttttgc catggaaagt gaattgagtg aaatcgaagc cctcttaatc cgtcaagtaa
    30481 cttcgttctt gtcctatctt gtgattgtgc tcttctctca tcagtcattt actgcagtag
    30541 ccaatggaca attgctaggt ctcatgattg tttttgcagc ctttgatatg atttcctact
    30601 tggcttatta tatcgctatc aatcgcttgc aaccagccaa ggctacaggc ttgaacgtga
    30661 gctatgtagt atggacggtc ttgtttgcag ttgttttctt gggtgcaccg ctagatatgc
    30721 tgaccattat gacgtcactt gtcgtcattg ctggagttta tattattatt aaagaataaa
    30781 ggagattcgt gtgaaagcca tcatcttagc agcgggattg ggaactcgct tgcgtcctat
    30841 gactgaaaat acccctaaag ccttggttca ggttaatcaa aaacctttga ttgagtacca
    30901 aattgagttt ctcaaagaaa aaggaatcaa tgacatcatc atcattgttg gttatcttaa
    30961 agaacaattc gattacttga aagagaaata cggtgttcgt ctcgttttca atgataaata
    31021 cgctgactac aataactttt actctctcta tcttgtaaaa gaagaattag ccaatagtta
    31081 tgttatcgat gccgataact atctctttaa aaatatgttc cgcaatgatt tgacacgttc
    31141 tacttatttt agtgtctatc gtgaagattg taccaacgaa tggttcttgg tctatggaga
    31201 tgactacaag gttcaaaaca ttattgttga tagcaaggca ggtcgcatcc ttagtggtgt
    31261 atccttctgg gatgctccaa cagcagaaaa aattgttagc tttatcgata aggcttatgc
    31321 aagcggcgaa tttgttgatc tctattggga caatatggtt aaggataaca tcaaagagct
    31381 agatgtctat gttgaagaat tagaaggcaa tagcatttat gagattgata gtgtccaaga
    31441 ctatcataaa ttagaagaaa ttcttaaaaa cgaaaattaa aagcttccaa catctgacaa
    31501 aatagtcgga tgtttttttg attttttacg aacttttacg aatagataga tgagtagaaa
    31561 aagaaatgga gttatttatg aaaatcacaa actatgaaat ctataagtta aaaaaatcag
    31621 gtttgaccaa tcaacagatt ttgaaagttc tagaatacgg tgaaaatgtt gatcaggagc
    31681 ttttgttggg tgatattgca gatatatcag gttgccgtaa tccagccgtt tttatggaac
    31741 gttattttca gatagacgat gcgcatttgt cgaaagagtt tcaaaaattt ccatctttct
    31801 ctattttaga tgactgttat ccttgggatt tgagtgaaat atatgatgcg cctgtacttt
    31861 tattttacaa gggaaatctt gacctcctga aattcccgaa ggtagcggtc gtgggcagtc
    31921 gtgcttgtag taaacaggga gctaaatcag ttgaaaaagt cattcaaggc ttggaaaatg
    31981 aactggttat tgtcagtggt ctggccaagg gcattgacac agcagctcat atggcagctc
    32041 ttcagaatgg cggaaaaacc attgcagtga ttggaacagg actggatgtg ttttatccta
    32101 aagccaataa acgcttgcaa gactacatcg gcaatgacca tctggttcta agtgaatatg
    32161 gacctggcga acaacctctg aaatttcatt ttcctgcccg taatcgcatc attgctggac
    32221 tttgtcgtgg tgtgattgta gcagaggcta agatgcgttc aggtagtctc attacgtgtg
    32281 agcgagcaat ggaagaagga cgcgatgtct ttgctattcc tggtagcatt ttagatggac
    32341 tatcagacgg ttgccatcat ttgattcaag aaggagcaaa attggtcacc agtgggcaag
    32401 atgttcttgc ggaatttgaa ttttaaaaat gacctaagct agaattctaa gaaaaaatca
    32461 attttaagag aaaatgaacc caacatttcc ataataaaac gcatattagc aagtttttaa
    32521 cacttgataa tatgcgtttt ttctaagtgg attagtagag tagaggattt ttctcatata
    32581 atactcttcg aaaatctctt caaactacgt cagcttccat ctgcaacctc aaaacagtat
    32641 tttgagctga cttcgtcagt cttatctaca acctcaaagc agtgctttga gcaacctgtg
    32701 gctagcttcc tagtttgcgc tttgattttc attgagtata agggaaagta tagtgaattg
    32761 aaataagatg tgaacaactc tatcaggaaa gtcaaattaa tttatagaaa tattttagca
    32821 gccaaggtgc actgttatag attcaataca ctataattta gtgtaattga gaaaggagaa
    32881 atgattgtga ttgatgttgg ctaggttatg ttcaatgatt cctaccgtct caaatcttgt
    32941 cagtaaggaa aaataaattc ttcaaaagta gagattacaa ggcttgttta agaaagaatt
    33001 caaagacctt gacaaataaa aataaaatgg ttattataaa aaatggtctg aaatagatga
    33061 tgatactttt cgaaaatctt tttacgtcag ctcagctttg ccttgctgtg ttttgagcaa
    33121 gctacggtta gcttccgagt ttgattttca tttactagaa atgaaactga tgagagatat
    33181 cagtagacat ttgagtcagg atattatgga aaatgataaa aagagctcgt gagattggca
    33241 tatcagacta ctaaagtatt gagtttgtta ggattttagc gactagttag ctgggaaagg
    33301 aagatatttg tgacaaataa taaactgtat tcgttgatag aatttagaaa taaaatatat
    33361 gaagaattag aactttccag aagtgattta gcgattttac tatgtgccat gcttatcgcc
    33421 tctatcggat taaatatgga ttcgactccc gtgattattg gagccatgtt aatctctcct
    33481 ttgatgacac ctattctggg agtggggctc tctctagcta tatttgattt taaattgtta
    33541 agaaaatctt ttaaaatatt agctattcaa attcttgcca gtttaatagc ttcaacactt
    33601 tatttttatc tttctcccat ttcgtatgct agttcggaaa ttgttgctag aacctctccg
    33661 actatttggg atgttctcat tgcttttgta ggagggatag caggtattat tggtgctagg
    33721 aaaaaagaga ccaataatat tgttcctggt gttgctattg caaccgcctt gatgcctcct
    33781 ctttgtacag taggttatgc tattgcttct gctaatctaa aatttatcat aggctcctct
    33841 tacctattcc tcatcaattg tagctttatt gtcattgcga cttatatagg tgttaggttg
    33901 atgatggtta agaaacatta ttttaaagat aatgaagaag actctaaaat gcgtaggatt
    33961 ttgcttctag ttgctgtttt gctgatgatt ccgagtttca tctctgcaac gactttagtg
    34021 agagaaacgt tgaaaaaaga gtcccttaag aaatttatat cagagcagtt tcaggggcat
    34081 aatattttga aaaaaaccta ttctaaaaag actcataccc taaagctaac catttcagga
    34141 aattatttga cagaagaaga actcgatatg atttcaagta agagaggtga ctatggttta
    34201 agtgatgttt ctgttcaagt ttcacaattg tctgattcag aacaacttag taaggaagaa
    34261 ctggtggagt atttcttcca gtatatcaag gataaggaag caaaagaaaa ggaaaaagct
    34321 aataagtttt atacagagtc tgaggagcaa taatttcttg agaatagctg gtttctctcg
    34381 tgagtcttct atgtatatca aaggaagact gaggttttaa gtataaaact tttcttctat
    34441 tatagtagct aagtcttgac agggttcaaa aaatggttta cactttataa agtttattac
    34501 tttgaaaagg tgtgatactg tggctacggc aacaaaaaag aaaaaatcaa cagttaaaaa
    34561 aaatctagtc atcgtggagt cgcctgctaa ggccaagacg attgaaaaat atctaggcag
    34621 aaactacaag gttttagcca gtgtcgggca tatccgtgat ttgaagaaat ccagtatgtc
    34681 cgtcgatatt gaaaataatt atgaaccgca atatatcaat atccgaggaa aaggccctct
    34741 tatcaatgac ttgaaaaaag aagctaaaaa agctaataaa gtttttctcg cgagtgaccc
    34801 ggaccgtgaa ggagaagcga tttcttggca tttggcccat attctcaact tggatgaaaa
    34861 tgatgccaac cgtgtggtct tcaatgaaat caccaaggat gcagtcaaaa atgcttttaa
    34921 agaacctcgt aagatcgata tggacttggt cgatgcccaa caagctcgtc ggatcttgga
    34981 tcgcttggta gggtattcga tttcgcctat tttgtggaag aaggtcaaga agggcttgtc
    35041 agcaggtcgc gttcagtcca ttgcccttaa actcatcatt gaccgtgaaa atgaaatcaa
    35101 tgccttccag ccagaagaat actggacagt tgatgctgtc tttaaaaagg gaaccaaaca
    35161 atttcatgct tccttctatg gagtagatgg taaaaagatg aaactgacca gcaatgacga
    35221 agtcaaggaa gtcttgtctc gtctgactag taaagacttt tcagtagatc aggtggataa
    35281 gaaagagcgc aagcgcaatg ctcctttacc ctataccact tcatctatgc agatggatgc
    35341 tgccaataaa atcaatttcc gtactcgaaa aaccatgatg gttgcccaac agctctatga
    35401 aggaattaat attggttctg gtgttcaagg tttgattacc tatatgcgta ccgattcgac
    35461 tcgtatcagt cctgtagcgc aaaatgaggc ggcaagcttc attacggatc gttttggtag
    35521 caagtattct aagcacggta gcaaggtcaa aaacgcatca ggtgctcagg atgcccatga
    35581 ggctattcgt ccgtcaagtg tctttaatac accagaaagc atcgctaagt atctggacaa
    35641 ggatcagctc aagctatata cccttatctg gaatcgtttt gtggctagcc agatgacagc
    35701 ggccgttttt gataccatgg ctgttaaatt gtctcaaaat ggagttcaat ttgctgccaa
    35761 tggtagtcag gttaagtttg atggttatct tgccatttat aatgattctg acaagaataa
    35821 gatgttaccg gacatggttg ttggagatgt ggtcaaacag gtcaatagca aaccagagca
    35881 acatttcacc caaccgcctg cccgttattc tgaagcaaca ctgattaaaa ccttagagga
    35941 aaatggggtt ggacgtccat caacctatgc gccaaccatt gaaaccattc agaaacgtta
    36001 ttatgttcgc ctggcagcca aacgttttga accgacagag ttgggagaaa ttgtcaataa
    36061 gctcatcgtt gaatatttcc cagatatcgt aaacgtgacc ttcacagctg aaatggaagg
    36121 taaactggat gatgtcgaag ttggaaaaga gcagtggcga cgggtcattg atgcttttta
    36181 caaaccattc tctaaagaag ttgccaaggc tgaagaagaa atggaaaaaa tccagattaa
    36241 ggatgaacca gctggatttg actgtgaagt gtgtggcagt ccaatggtca ttaaacttgg
    36301 tcgttttggt aaattctacg cttgtagcaa tttcccagat tgccgtcata cccaagcaat
    36361 cgtgaaagag attggtgttg agtgtccaag ctgtcatcag ggacaaatta ttgagcgaaa
    36421 aaccaagcgt aatcgcctat tctatggttg caatcgctat ccagaatgtg aatttacctc
    36481 ttgggacaag cctgttggtc gtgactgtcc aaaatgtggc aacttcctca tggagaaaaa
    36541 agtccgtggt ggtggcaagc aggttgtttg tagcaaaggc gactacgagg aagaaaagat
    36601 taaataagag gaggggcccg aaagtaaatt tcaggctcct tttggttaga acttgacaaa
    36661 atacatcatt ttatgcgaaa ctagaagaag attattttat actcaatgag aatcaaagag
    36721 caaattagaa agctagtcgc aggttgctca aagcactact ttgaggttga agatggaagc
    36781 tgacctggtt tgaagagatt ttcgaagagt attaactagg agaagttatg cgtcttatct
    36841 atctgataat tggtttttta tcactggcct tggctattgt tggggttgtt ttacccttgt
    36901 tgccgacaac gcctttcctt ttgttgtcta ttgcttgttt ctccagaagt tccaagcgtt
    36961 tcgaagactg gctttatcat accaagatct atcaaacata tgtagctgat tttcgcgaga
    37021 ctaagtctat tacgcgtgaa cgaaagaaaa aaatcatcgt ctctatctac gtcttgatgg
    37081 gaatttctat ttattttgca cctcttttac cagtcaaaat cggtctgggt gctttgacca
    37141 tctttattac ttattatctc ttcaaggtca ttccagacaa agaatagtta aaacagtagt
    37201 tatttgcctt gataaaattg aaagcatatt cataacaata tgatataata aatttgaagt
    37261 aatattcaag gagaatcaaa tgatttacga attttgtgct gaaaatgtga ctttacttga
    37321 aaaagcgatg caggctggag ctcgtcggat tgaactctgt gataatctag cagttggtgg
    37381 gacaacaccc agctatggag tgactaaggc agcggttgaa ctggcagcta actacgatac
    37441 aaccatcatg accatgattc ggccacgtgg tggtgacttt gtctataatg acctagaaat
    37501 tgctatcatg ctagaagaca ttcgtttgac tgctcaggct ggaagtcaag gggttgtatt
    37561 tggaacttta actgctgata aaaagttgga taagcctaat ctggaaaagt taattgctgc
    37621 atcaaaagga atggaaattg tctttcacat ggcctttgat gaactaagtg atgaagatca
    37681 accggaagct attgactggc tcagtcaagc cggtgtcact cgtatcctaa ctcgtgctgg
    37741 tgtgtctggc gactccttag aaaaacgttt tgttcactat cacagaattt tggagtacgc
    37801 taaaggtaaa attgaaattc taccaggtgg ggggattgac cttgaaaacc gtcaaacctt
    37861 tatcgaccag ttgggggtaa cacaattgca tggtactaag gttgtttttt aaaaaataga
    37921 aaggaactgc tagctttggg tagcagtttt cacttatgtt tgaaattttt aaatcctatc
    37981 aatttaatca agaaaaggct catgcttttg gttttgtagt aaatagtgga gtctggacct
    38041 atagttgcca gattttgcaa ggtgactttg tcatgactgt gtccatcact gctgataatg
    38101 tgaactttca agtctttgac caagagactg atgacctcta tcctcacgtt catatggaaa
    38161 gcatgagggg aagttttgtc ggaaatgtcc gtgaggcttg tctggagatt ctttaccaga
    38221 ttcggaaggc ttgttttgat gtgcaagatt ttatctgtca tcagactaag cgtattatga
    38281 ctcaagttca ggaaaagtat ggaaaccagt tggagtatct gtgggaaaaa tcgcctgata
    38341 cagctgtatt gcgccatgaa ggcaatcaaa agtggtatgc cgtcttgatg aaaatctctt
    38401 ggaataagct ggaaaagggc agagaaggac aagtggaagc agtcaacctc aagcatgacc
    38461 aagtagctaa tttactttca caaaagggga tttatccagc ctttcatatg agcaagcgct
    38521 actggattag tgtgtccctt gatgatactt tatcagatga agaagtactg gaattgatag
    38581 aaaaaagttg gaacttaacc tctaaaaaat gaaatatttt aataattttc atgaactttc
    38641 aattagctaa atattcttta ctgaagaaat tttcagaaaa tatagaattt accacaccag
    38701 gggaatatgg tgccatcttc aaatacctga ttgagaatgt caagacggat cgtcagatca
    38761 tctgttcgcc tcactgtcat gatgacctcg gaatggcagt ggcaaatagc cttgctgctg
    38821 tcaagaatgg tgcaggacgt gttgaaggga ctatcaatgg tattagggag cgagctgaaa
    38881 atgctgcttt ggaagaaatt gcagtggctc tcaatattcg ccaagattac taccaagtag
    38941 aaaccagtat tgtcctaaat gagaccatca atacgtcaga aatggtttct cgcttctctg
    39001 gtattccagt tcctaaaaac aaagccgtcg ttggtggcaa taccttctcc catgaatctg
    39061 gtattcacca agatggagtc cttaaaaatc ctctcactta tgagatcatc acacctgaat
    39121 tggttggtgt taagattccg cttggaaaat tatctggtcg ccatgctttt gttgagaaac
    39181 tgagagaatt ggccctagat tttacagaag aggatatcaa accactcttt gctaagttca
    39241 aggcactggc cgataagaag taagaaatca cagatgcaga tactcgagct ttggtagctg
    39301 gaaccatggt tgaaaatcca gaaggcttcc actttgatga tttacaactt caaactcatg
    39361 cagataatga cattgaagcg ctcgttagcc tagccaatat ggatggtgag aaagtcgaat
    39421 ttaatgcgac agggcaaggt tccgttgaag caatctttaa cgctatcgat aagttcttta
    39481 accaatctgt tcgtttggtg tcctacacta tcaatgcggt aacagatgga atcgatgccc
    39541 aggatcgggt tttggtcact gttgaaaaca gagatacaga aaccatcttt aatgcagcag
    39601 ggcttgattt tgatgtgttg aaggcttctg ctattgccta tataaacgct aatacctttg
    39661 ttcaaaaaga gaatgcatgt gagatgggac gcagtgtttc ttaccacgat atgcctagtg
    39721 tgtaaaggag aaggctatgg caaagaaaat agtagctcta gtaggagacg gaattggccc
    39781 agaaatcatg gaggctggtt tagaagttct ggaggctcta gctgaaaaaa caggttttga
    39841 ctatgagatt gacagacgac cgttcggagg tgcagatatt gatgcagcag gacctccctt
    39901 acctgatgaa acccttaagg caagtaggga agcagatgtt atcctactag cagctatcgg
    39961 tagtcctcag tatgatggag cagcggttcg ccctgaacaa ggcctgatgg ctctccgtaa
    40021 ggaactcaat ctttacgcta atattcgtcc tgtaaaaatc tttgacagtc tcaagtattt
    40081 gtcaccactt aaaccggaac gaatttctgg tgtagacttt gtcgtggtgc gtgaattgac
    40141 aggcgagatt tactttggag atcatattct tgaagagcgc aaagcgcgtg atatcaacga
    40201 ctatagctat gaggaagtgg agcggattat tcgcaaagcc tttgaaattg caagaaatcg
    40261 cagaaaaatc gttactagta tcgataagca aaatgttcta gcgacctcaa aactctggcg
    40321 gaaagtagct gaggaagtcg cacaggattt cccagatgta accttggaac accagctggt
    40381 agactcagct gttatgctta tgattagcaa tcctgctaag tttgatgtta ttgtaacgga
    40441 gaatcttttt ggagatattt tatctgatga atcaagcgtc ttatctggta cacttggggt
    40501 tatgccatca gccagtcatt ctgaaaatgg accaagtctc tatgaaccta tttacggttc
    40561 agcacctgat attgcaggtc aaggaattgc caatcctact tccatgattt tatcagttgc
    40621 catgatgttg agagatagtt tcggacgtta tgaggatgca gagcgtatca aacgtgctgt
    40681 tgagacaagt ctggcggcag gaattttaac gagagatata ggggggcagg cttcaacaaa
    40741 ggaaatgacg gaagctatta ttgcaaggtt atgaagttag acgaaaaaat tactctagtc
    40801 cttttgattt ggaatgtcat cattttcttg atttatggca ttgacaaatt caaggcaaga
    40861 agaagaactt ggcgcatcca agagaaaatc ttactcattt tagcccttac ttgtggtggt
    40921 tttggggcct ggttagctgg aatcactttt caccacaaga ctcgaaaatg gtactttaaa
    40981 acagtttggt ttcttgggat ggtgaccaca ctagtagcct tatattttat ttggaggtaa
    41041 tggatggcag ggtcttcgag ggaacacgct gcttgggctc tagcgaacta tggttttaag
    41101 gtcgtgattg caggaacttt cggtgacatt cattacaata atgaactcaa taatggcctg
    41161 ttgccaatcg ttcagcctag agaggttaga gagaaactag cccagctaaa accaaccgac
    41221 catgtaactg tggacttgga acaacaaaaa atcatctcac cagttgaaga attcaccttc
    41281 gagatagata gcgagtggaa acataaactc ctaaatagtt tggatgatat cggtattacc
    41341 ttgcagtatg aagagttgat tgctgcttat gaaaaacaac gaccagccta ctggcaggat
    41401 tagaaaaaat agaaaaggaa atatagtaaa ctgaaataag atgtaaacaa atgaattaga
    41461 gcttaacatc catttccagc aattttttag aaactacagt ggactattct ggattcaaca
    41521 cattataaaa ttatgacaag acacattcac aagaaggcta cgacatttta aaaggtgagg
    41581 gcggatgtat cgtttgccct actaaagttg gttacattat catgaccagt gacaaggcag
    41641 gacttgagcg taagttcgca gccaaagaac gtaagcgtaa caaaccaggt gttgttctct
    41701 gcggtagcat tgatgaactt tgcgctttag cgcaactcaa cccagaaatt gaagcattct
    41761 actaaaaaca ttgggatgaa gatattcttc ttggttgtat ccttccttgg aaaccagaag
    41821 cctttgaaaa actcaaagca tacggggatg gccgtgaaga acttatgact gatgtacgtg
    41881 gtactagctg ttttgttatc aagtttggaa aagcaggtga acaattggct gccaagcttt
    41941 gggaagaagg taaaatggtc tacgcctcat ctgcttcaat gacaaaacga ttgaaactcg
    42001 ctatgagcaa ggtgtaatgg tgtctatggt cgataaggac ggcaaactca tcccagaaca
    42061 aggaggagca cgttcaactt caccagctcc agttgtgatc cgtaaagggc ttgacattga
    42121 tatagtaaaa tgaagtaaga gcaggacaaa ttaatcagga cagtcaaatc gatttctaac
    42181 aatgttttag aagtagaggt gtactattct agtttcaatc cactataaaa tcatgatgca
    42241 cctgtcagat acttttaact catgggacta ccgtcaggtt gagtattatt aggatagaga
    42301 agaagtctag tgttaaggac attacagctc ataatactag gcttttttat agaaattctt
    42361 ttctttttct ataggatatg gtattctatg tagaaaatat atgttaataa gtaatgccaa
    42421 tatttaaaca tcattagtaa aaggagttag attgatgaat aaaagaaaag ttagtttaga
    42481 agatttttat aaatggtata gtctaaataa agaagagtta ttaaataagg caactgttgg
    42541 tgaaaagttt aatgataaat taaaagaaga gtttctccag gaatggcctt tggataggat
    42601 tttaacaatg tcaatcgatg aatatgtaat aggaaaggga cagcaaaata agtctttatg
    42661 ctacgctctt gagaagggaa aatacaaaaa tctatttctt ggaatttctg gtggctcagc
    42721 ttcaaaattt ggtatttatt ggaataaaaa aacaaacaaa tataaagatc aagctaataa
    42781 tgagatttca gagttggatc agcgattttc aaaattaaaa tcagatttgt atgaaattat
    42841 caaagaaggt attcgtttta actttgaaaa tcctattttt gatatgaaaa gatcaacaaa
    42901 tgaatttatt ggtcgttctg ctatggtgac aaaattactt tgtatctata ctgagggaga
    42961 tcctttcttt ggtgtaaata ttaatagtca gaaagaattt tggaaccact ttgtttctca
    43021 gacaaatcaa ggtggacctt atctgcaaaa tcataaaata attgaactga tgtccaaaac
    43081 ttatcctgag ttggagccat cgaaattagg aactatgctt tttgagtatt ctaagctttt
    43141 tatggaaaat aaggaagaca atagtacaat ggattcatca aacaattttc gtcatcaatt
    43201 aactcaatct ctattaaagt ctccaaacct tatccttcgt ggtgctcctg gcacaggaaa
    43261 gacttatctt gctaaagaaa ttgccaaaga attaacggat ggcaacgaag atcaaatcgg
    43321 atttgtacaa tttcacccat catatgatta tacggatttt gtagaaggtt taagaccagt
    43381 atcaaatggg gatggagcta ttgagtttag gctacaggac ggtattttta aagatttttg
    43441 tcagaaagca aaagaaaccc aattgattgg aggacaagat aattttgatg aggcttggga
    43501 ttcttactta gaatatataa atgttgctga agaaaaagaa tatataacaa aaacatctta
    43561 cttatctgtt aatagtagac aaaatttgtc agtaaattat gatagtggtg ttccaggatg
    43621 gtcactacct agcaaatatg tttacgagtt gtataaagat aaaaattata ataagcaaga
    43681 atactacaaa agtggtggaa aaactgtcct agaaacattg agaaagagat ttggtttgaa
    43741 agactatgtt tccccaacag aaattgatac tgataagaaa ttcgttttca tcatcgatga
    43801 aatcaatcgt ggggagattt ctaagatttt tggcgaactc tttttctcta tcgaccctgg
    43861 ctatcgtggt gaaaaaggaa gtgtttctac ccaatatgca aatctacacg aaactgatga
    43921 aaagttctat atccccgaaa atgtttacat catcggaact atgaatgata ttgatcgttc
    43981 agtggatacc tttgattttg ctatgcgtcg tcgttttcgt tttgttgaag ttactgccga
    44041 gggtcaagtt ggcatgttgg ataaagagct gaatatccat gcagaagaag caaaaattcg
    44101 tctaagaaac ttgaacgctg ctatcgaaaa tgttcaggaa ttaaacagtc attatcatat
    44161 tggaccaagt tattttctta agttgaagga tgtagatttt gactatgaat tactctggtc
    44221 cgattatatt aagcctctcc tagaagacta cttgcgaggt tcttatgatg aggttgaaac
    44281 tttggaaact ttgaaaaaag catttgaact gacaaataat gagcaaaaag atcaggcagt
    44341 agctgatgac aatgaaagcg atgaaaacga tgaaaacgat gatgcggatt actgataatc
    44401 aacacaagat tattaaagaa aaatttgttg aagaatatcc taaactaagc aatcttcttt
    44461 tagacagaac cttggaaagt ctatcccaag atgaacgtat tttcattttt ccaaatgatt
    44521 tgactcatac tccagatttg gataaaaaac aaaagatttt ggaaacgatc aatcaggaaa
    44581 tcaagacagg aaatgtgatt gattttcttg gatatggtca ggaaagatta acgatttcct
    44641 cacgattttc tgatgagagt aatgaccatt ttttgcatta tctcttaaac aaggttcttc
    44701 atatcaatct cactagttta gatgttgctt tgtctcgtga agagaggctt tatcaacttt
    44761 tgatgtatct cttccccaag tatctacaag ctgctattcg aaaaggtctt tataaggaat
    44821 atcatcgatt ttctcataac gacagtcatg ttaagggagt gattgatgta agaaaccatc
    44881 tcaagaaaaa tcttcctttc acgggaaata ttgcctacac aacgagagag ttcacctatg
    44941 ataatcccct catgcagttg gtccgtcaca ctattgaata cattaagaat cagaaaagca
    45001 ttggtcaagg ggtactagat aatctctcaa ctagtcgtga aaacgtatct gaaatcgtgc
    45061 gtgtaacgcc ctcttataaa ctagctgatc gtgctaagat tattcgggga aatcaatcta
    45121 aacctatacg tcatgcatac tttcacgagt acagaaactt acaagaactt tgtctgatga
    45181 tcctaaacca agaaaagcac ggtttagggt atcaagatca aaaaatctat ggtattctct
    45241 ttgatgttgc ctggctttgg gaagagtatg tttacacctt gttgccaaaa ggttttgtac
    45301 atcccagaaa taaggataag acggatggaa tttcagtatt ttctgttggg aaacgaaaag
    45361 tatatccaga tttttatgac agagaacgaa agattgttct agatgcaaaa tataaaaaac
    45421 tggaattgac tgaaaaagga atcaatcgtg aggacttatt ccagctgatt tcctattctt
    45481 atattttaaa agctgagaag gctggacttg tttttccgag caaggacaag gttattgata
    45541 atgagatggg aaacctagca ggttatggag ctttgttgaa gaaatggtct atccaaattc
    45601 ctcagaagtc ttcatcttat agtgcatttt gtaaaatgat ggaaaattca gattatagtg
    45661 cattttgtaa aatgatggaa aattcagaag aaaattttaa agcgattatt gatgaagaag
    45721 tggggagaaa ataatttccc ctctttcttt tatttttaac acaagtaaaa cccgaacaat
    45781 atatttcaat tcataaaata atttgacaaa aactgtactt tggtttataa taaattaagg
    45841 aaacgtcgga aaagacgtag tgatgcgaga gcataggtag gtcattacaa aagaaacgag
    45901 acatcgatat gttaaatgaa tttccaattt ttgattacga agatattcaa ttgattccaa
    45961 ataaatgtgt cattaaaagc cgtgcagaag cggatacaag tgtcacttta ggaaatcaca
    46021 cctttaaact acctgttgtg ccagcgaata tgcagacgat tttggatgaa aatgtagcag
    46081 agcaactggc taaaggtggt tacttctaca ttatgcaccg ttttgatgag gcaggacgca
    46141 ttccttttat taaacaaatg cacgatcaag ggctcattgc ttccatctct gtcggtgtta
    46201 aggactatga gtatgatttt gttagccagc tcaagactga tgctcctgag tatatcacta
    46261 ttgatattgc tcatggtcat gcggatagcg tgatttctat gattcaacat atcaagaaag
    46321 aattgccaga tacctttgtc attgctggaa atgtgggaac accagaagct gtgcgtgaat
    46381 tggaaaatgc tggtgcggat gctactaagg tcggaatcgg tcccggtaag gtttgtatca
    46441 ccaaggttaa gactggtttt ggtacaggtg gttggcagtt ggctgctcta cgctggtgtg
    46501 ccaaggctgc gcgtaaaccg attatcgctg atggaggaat tcgtactcac ggtgatattg
    46561 ctaagtctat ccgcttcggt gctagcatga tcatgattgg ttccctcttt gcaggacaca
    46621 ttgaaagtcc agggaaaacg attgaagtcg atggtgaaca gttcaaagaa tattatggtt
    46681 cagcctcaca atatcaaaaa ggtgcttaca aaaatgtgga aggcaaacgt atcttgcttc
    46741 ctgctaaagg tcatctgcaa gacactttaa ctgagatgga acaagacctt caaagtgcta
    46801 tttcttatgc aggtggacgt caggttgctg accttaaaca cgttgattat gtgatcgtga
    46861 aaaactccat ctggaatggg gatgcttccc actaagacgg ggtctttgac caaaaaactt
    46921 gttattagag caaatttctg ttataataaa acaagtttcc acccttagtg taatggatat
    46981 cacgtaagat tccggttctt gagatggggg ttcgattccc tcagggtgga tgtaaacatc
    47041 ctaaaaagcc tttaataggg cttttttctt tatcctgacc caaatttgcc gctaaaactg
    47101 agaatctctg tctaaagaat tcccaaatgc tttccagtcc cgttttagag tgactcaggg
    47161 ggcttttttt gatataataa aaaggactgt tatcagttag aaagaggttg gtatgaaaga
    47221 attacaaact gtactaaaga atcattttgc aatcgaattt gcagacaaaa atttactgga
    47281 aactgccttt actcatacga gttatgccaa tgagcaccgc ctcttaaaaa tttcacacaa
    47341 tgaacgcttg gaatttttag gagacgctgt tctacagtta ttgatttcag aatatctata
    47401 taaaaaatat cctaaaaagc ctgaaggtga cctatcaaaa ctccgtgcta tgattgtccg
    47461 tgaggagagt ttagctggtt ttgcgcgtga ttgccagttt gaccagttta tcaagttggg
    47521 taaaggggaa gaaaaatcag gtggtcgcaa tcgtgacacc attcttggtg atgcctttga
    47581 agcctttctt ggtgcccttc ttttggataa ggatgtggcc aaggtcaagg aatttatcta
    47641 tcaagtcatg attcctaagg ttgaagcagg cgaatttgag atgattacag actataaaac
    47701 ccatctccaa gagttacttc aggtcaatgg tgatgtggct attcgttatc aggtgatttc
    47761 tgaaacaggg cctgctcacg ataaggtctt tgatgtagaa gttcttgttg aaggtaagag
    47821 catcggtcaa ggccaaggtc gttctaagaa attagcagag caggaagctg ccaaaaatgc
    47881 cgttgagaag gggctggatt catgtattta aaggaaatcg aaattcaggg gttcaagtct
    47941 tttgctgata agaccaaggt cgtttttgac caaggtgtga cggcagttgt tggacccaat
    48001 ggatctggaa agtccaatat tacagaaagt ctgcgttggg ctttggggga gtctagtgtc
    48061 aagagtctcc gtgggggcaa gatgccggat gtcatctttg ctggaacaga aagtcgcaaa
    48121 ccgctcaatt atgcttctgt agttgtgact ctggataatc atgacggatt tatcaaggat
    48181 gcaggtcaag aaatcagggt ggaacgccat atctatcgta gtggagatag cgaatacaag
    48241 attgacggca agaaagtccg tctgcgtgat attcatgacc ttttcttgga tacaggtttg
    48301 ggacgagatt ccttctctat tatttcccaa gggaaggttg aggagatttt taattccaag
    48361 tctgaggaac gacgagctat ttttgaagaa gctgctggag ttttaaaata caagactcgc
    48421 agaaaagaaa ccgagagtaa actgcagcaa actcaggata atctggaccg cttagaggac
    48481 attatctacg agttggataa tcaaatcaag cctcttgaga agcaagctga gaatgcccgt
    48541 aaatttctag acttagaagg tcaacgcaag gctatttact tggatgtatt ggttgctcaa
    48601 atcaaggaaa ataaggcaga actagagtcg acagaagaag agttggctca ggttcaagaa
    48661 ctcttgatga gttattacca aaagcgtgaa aaattagaag aagaaaatca aactcttaaa
    48721 aagcaacgcc aagatttaca ggctgaaatg gccaaagacc aaggcagttt gatggacttg
    48781 actagtctga ttagtgattt agaaagaaaa ttagccctat cgaaactgga gtctgagcaa
    48841 gtggccttga atcaacagga ggcacaagct cgtttggctg ctttggagga taagagaaat
    48901 tcactcagca aagaaaagtc tgataaagaa agctctttag ctctgttaga gggaaatcta
    48961 gtccaaaata atcaaaaact caatcgttta gaagctgaat tgctggcttt ctcagacgat
    49021 cctgatcaga tgattgagct cttacgtgaa cgctttgtag ctcttttaca agaagaagcg
    49081 gatgtctcaa accagttgac ccgtattgag aatgagttgg aaaatagtcg tcagctttct
    49141 caaaaacaag cagatcaact agaaaagctg aaagagcaat tagctatagc taaagagaag
    49201 gctagtcagc aaaaggaaga gcttgaaact gccaaggagc aggttcagaa attattggct
    49261 gactaccaag ctattgccaa ggagcaagag gagcagaaaa cttcctatca agctcaacaa
    49321 agccaactct ttgaccgtct ggataatctc aaaaacaagc aggccagagc tcaaagtttg
    49381 gaaaatatcc tgagaaatca tagtaacttt tatgcaggtg ttaagagtgt tctccaagaa
    49441 aaagatcgcc taggtgggat tattggtgca gtcagtgagc atctgacctt tgatatgcat
    49501 tatcaaactg ccctagaaat tgcgcttgga gcgagtagcc aacatatcat cgtagaagat
    49561 gaagagtcgg caaccaaggc tattgacttc cttaaacgga acagagccgg tcgtgcaacc
    49621 tttcttcctt tgaccactat caaggcgcgt acgatttcta gtcagaacca agatgctatc
    49681 actgtaagcc caggtttcct tgggatggca gatgagttgg tgacttttga tactagactg
    49741 gaagccattt tcaagaactt gctagctacg acggctatct ttgatgccgt agaacatgcg
    49801 cgtgcagcag ctcgccaagt tcgttatcag gttcgtatgg tgacattgga tgggacagaa
    49861 ttacgcacgg gtggttccta tgcgggtggt gccaatcgcc aaaataacag tattttcatc
    49921 aagccagaac tggagcaatt acaaaaagaa attgctgcag atgaagcaag cttgcgttca
    49981 gaagaagcgg ctttgaagac cttacaagac cagatggcta cattgacaga aagattagaa
    50041 gtcatcaaat ctcaaggaga acaggcacgt attcaggagc aaggcttgtc cctcgcttat
    50101 cagcaaacta gtcagcaagt tgaagaactg gaaactcttt ggaaactcca agaagaggaa
    50161 atagatcgtc tttccgaggg agattggcaa gcggataagg aaaaatgcca agatcgtctt
    50221 gctgcaatcg ccagtgacaa gcaaaatctg gaagctgaga ttgaagagat taagtctaac
    50281 aaaaatgcca tccaagaacg ctatcaaaac ttgcaggaag agctagcgca agctcgtttg
    50341 cttaagacag aactgcaagg gcaaaaacgt tatgaagttg ctgatattga acgcttaggc
    50401 aaggaattgg acaatcttga ttttgaacaa gaggaaatcc agcgccttct tcaagaaaag
    50461 gttgataatc ttgagaaggt tgatacagaa ttgctcagtc aacaggcgga agaagccaaa
    50521 actcagaaaa caaatctcca acaaggtttg attcgcaagc agtttgagtt ggatgatatt
    50581 gaaggtcaac tggatgatat tgctagtcat ttggatcagg ctcgccaaca gaatgaagag
    50641 tggattcgca agcaaacacg tgctgaagcc aagaaagaaa aggtcagcga gcgcttgcgc
    50701 tatttacaaa gtcaattaac agaccaatat cagattagct atactgaagc tctagaaaag
    50761 gcgcatgaat tggaaaacct caatctggca gagcaagagg tgcaggattt agagaaagct
    50821 attcgctcac tggggcctgt caatatagaa gctattgacc agtacgaaga agttcacaat
    50881 cgtctggatt tcctaaatag ccaacgagat gatattttgt cagcgaaaaa tctgctcctt
    50941 gagaccatta cagagatgaa tgatgaggtc aaagatcgct ttaaatcaac ctttgaggcc
    51001 attcgtgaat cctttaaagt gaccttcaaa cagatgtttg gaggaggtca ggcagactta
    51061 atcttgactg agggagactt gttgacagct ggggttgaga tttctgttca acctccaggt
    51121 aagaaaatcc agtcgcttaa cctcatgagt ggtggtgaaa aagccttatc agctcttgct
    51181 ttgctttttt ctatcattcg agttaagact atcccatttg tcatcttgga tgaggtagag
    51241 gccgcgctgg acgaagccaa tgttaaacgt tttggggatt acctcaatcg atttgacaag
    51301 gacagccagt ttatcgtcgt aacccaccgt aagggaacca tggcagcggc tgattctatc
    51361 tatggagtga ccatgcaaga atcgggggtc tcaaaaattg tttcggttaa gttaaaagat
    51421 ttagaaagta ttgaaggatg acaattaaac tagtagcaac ggatatggac ggaaccttcc
    51481 tagatgggaa tggacgcttt gatatggatc gtctcaagtc tctcttggct tcctacaagg
    51541 aaaaagggat ttactttgcg gtggcttcgg gtcggggatt tctgtctctg gaaaaattat
    51601 ttgctggtgt tcgtgatgac attattttca tcgcggaaaa tggcagtttg gtagagtatc
    51661 aaggtcagga cttgtatgaa gcgactatgt ctcgtgactt ttatctggca acttttgaaa
    51721 agctgaaaac gtcaccttat gtagatatca ataaactgct cttgacgggt aagaagggtt
    51781 catatgttct agatacggtt gatgagacct atttgaaagt gagtcagcat tataatgaaa
    51841 atatccaaaa agtagcgagt ttggaagata tcacagatga cattttcaaa tttacaacca
    51901 acttcacaga agaaacgctg gaagctggag aaacttgggt aaacgataat gtccctggtg
    51961 tcaaggctat gacaactggc tttgaatcta ttgatattgt tctggactat gtcgataagg
    52021 gagtggctat tgttgaatta gctaaaaaac ttggtatcac aatggatcag gtcatggctt
    52081 ttggagacaa tcttaatgac ttacatatga tgcaggttgt gggacatcct gtagctcctg
    52141 aaaatgcacg acctgaaatt ttagaattag caaagactgt gattggtcac cataaggacc
    52201 agtcggttat agcttatatg gagggcttat aatggcagat ataaaattaa tcgcattgga
    52261 cttggacggt accttgctga ctactgataa aaggctgacg gatcgcacca aggcaacttt
    52321 gaaagctgcg agtgatcgtg gtatcaaggt cgtattgaca actggtcgtc ccttaaaagc
    52381 catggacttc tttctccatg agttagggac tgacggccat gaagatgagt ataccattac
    52441 ttttaatggt ggattagttc agaaaaatac aggagaaatc cttgataaaa cagtcttttc
    52501 atatgatgat gtggcacgtt tgtatgagga aacagagaaa ttatcactgc ctcttgatgc
    52561 catctcagaa ggaacagttt atcaaatcca atcggaccaa gaaagtcttt atgccaaatt
    52621 caatccagct ttgacttttg ttccagttga ctttgaagac ctgtctagtc aaatgaccta
    52681 caacaaatgc gtgactgcct ttgctcaaga acccttggat gcagccattc agaagatttc
    52741 tccagaattg tttgaccaat atgaaatctt taaatcacgt gaaatgttgc tagaatggtc
    52801 accaaagaat gttcataaag caacaggttt ggcaaaacta attagccatc ttggaattga
    52861 tcaaagtcaa gtgatggcct gtggtgacga ggccaatgac ctctctatga ttgaatgggc
    52921 agggctcggt gttgccatgc aaaacgctgt tcctgaagta aaggcagccg caaatgtagt
    52981 gacgccgatg accaacgatg aggaagctgt cgcctgggct atcgaagaat atgtgctaaa
    53041 ggagaactaa gatatgggat tatttgaccg tctattcgga aaaaaagaag aacctaaaat
    53101 cgaagaagtt gtaaaagaag ctctggaaaa tcttgatttg tctgaagatg ttgatcctac
    53161 cttcacagaa gttgaagaag tttctcaaga agaagctgtg cttgaagaaa gtcaggattc
    53221 agagccagct gtaggggttt ctcaagaaga aatagaagaa ttaccagagg aagagaagct
    53281 tgagcacgaa ggaactatag aagaaaataa ttctgaagtt cttgaagcag aaaggcctca
    53341 aacagaagaa actgttcagg aaaaatatga ccgcagtctt aagaaaactc gcacaggttt
    53401 cggtgcccgc ttgaatgcct tctttgctaa cttccgctct gttgacgaag aatttttcga
    53461 ggaactggaa gaactgttga ttatgagtga tgttggtgtc caagtcgctt ctaacttaac
    53521 ggaggaacta cgttacgaag ccaagcttga aaatgccaag aaacctgatg cacttcgtcg
    53581 tgtcatcatt gagaaattgg ttgagcttta tgaaaaggat ggtagctacg atgaaagcat
    53641 ccacttccaa gataacttga cagttatgct ctttgttggt gtgaatggtg ttgggaaaac
    53701 aacttctatc ggaaaactag cccaccgcta caaacgagct ggtaagaagg tcatgctggt
    53761 tgcagcagat accttccgtg cgggtgcagt agctcagcta gctgaatggg gccgacgagt
    53821 agatgttcca gtagtaactg gacctgaaaa agctgatcca gccagcgtgg tctttgatgg
    53881 tatggaacgt gccgtggctg aaggtatcga tattctcatg attgatactg ctggtcgtct
    53941 gcaaaataag gataacctta tgactgagtt ggaaaagatt ggtcgtatta tcaaacgtgt
    54001 tgtgccagaa gcaccacatg aaaccttctt ggcacttgat gcatcaacag gtcaaaatgc
    54061 cctagtacag gccaaagaat tttcgaaaat cacaccttta acgggaattg ttttgactaa
    54121 gattgatgga actgctcgag gaggtgtggt tctagccatt cgtgaagaac tcaatattcc
    54181 tgtaaaattg attggttttg gtgaaaaaat cgatgatatt ggagagttta actcagaaaa
    54241 cttcatgaaa ggtctcttgg aaggtttaat ctaatcagaa gcaaaaatcc tgcaaggcat
    54301 aaacttgcag gaaatttttt tattcgaaac gaccatcttg acgataggcg atatctggtt
    54361 gccaagtcca tttggcaccg aatttttcaa gtaggtcaaa gctggcttga ggtcccatgc
    54421 ttccagcttt atagtcatga agtggggcac cattttcagc ccagagctct tcaatacggt
    54481 caatcaactt ccatgatgca ccaacttcat cccagtggct aaagttagtt gagttgttat
    54541 ttaggacatc ataaatcaat ttctcgtatg gttctggaga agcaccagtt gcagtcgcat
    54601 ctgtacggta atcaagtgag ttaggagcca agttaaattc ttctcctact tgcttcccat
    54661 ttaggctaag agagaagcct tctgttggtt gaatatagat ggtcaaaata tttggagcaa
    54721 gtggttctcc aaagatagaa tccatttgtt taaagacgat gttgacatga gttccttttt
    54781 cagtcagtcg tttacctgta cggaaaaaga aaggaacact acggaatcga tcgctgtcta
    54841 caaagaaggc accagatgta aaggtttcag ttgttgattc tggattcaca tttggctcgc
    54901 tacgataaga gatgtatttc atgccatcaa tcttaccaga gcggtattgc ccacggataa
    54961 agtgttcttt gagttcttca tcagttggat gatagaggtt tttaaagacc ttaatctttt
    55021 cagcacgaat ctcgtctttt gtgaagattg ctggtttgtc catggcgagg agcgaaagaa
    55081 gttgtagagt gtggttttgg accatgtcac ggagggcacc ggattggtca tagtagccac
    55141 cacgttcttc tacacccaag cgctccgcaa aggtaatttg aacattgtcg ataaaatcct
    55201 tgttccaaac gttttcaaaa atcaagtttg caaagcgaac tgcaaagatg ctttggatca
    55261 tttccttacc aagataatgg tcgatacgga aaatttgttc ttcgtcaaat gttgctagga
    55321 gttcgtcatt caacttgctt gcagttgcgt aatctgtacc aaatggtttt tcaacgatca
    55381 agcgctcaaa acctttgcca tcgacaatgt tttcagattt gaggtgtttg gcaatggttc
    55441 caaagaactg aggtgccata gacaagaaga agagcttatt gtgttcagct tggtattttt
    55501 cattaagctc agcttgtaat tgacgcaaag caatataatg ttccgaatca ttgacatcat
    55561 ggctttgata gtagaagtgg ctagcaaatt cttgggcttg ctcggtacta tctgccaaat
    55621 caaggatgga ctcgacaact acagattcaa aatattcctt actccaaggt ctacgggcag
    55681 ttccaataac ggcaaagtgc ttggaaagat tgccggattg atatagtcta aaaagggaag
    55741 ggtagagctt gcgtttagcc aggtctccac tcgcaccgaa aattgtaaca ataaccttag
    55801 atgacatcta gctacctaat ttctattttt attcctagtc ttgctaggta tgaggaaacc
    55861 tcatttcata aacttaattc taacataatt ttccgaaaaa tcaaaaattc caatacgagg
    55921 tagaaaaact gcaaggaaat ccttacagtt tgagtttaga cgtttaagac cttatctaag
    55981 aactctttca gacgagggtg ttgtgggtta tcaaagattt ggtcaggtgt tccgtcttca
    56041 aggaactcgc catctgcagt aaagataacg cggttggcaa cctgacgagc aaatcccatc
    56101 tcatgggtta cgataatcat ggtcatgcct tgctcagcca attccttcat aacgttaagt
    56161 acgtctccaa ccatctcagg gtcaagggca gaagttggtt catcgaagag catgatgtct
    56221 ggattcattg ctaggccacg agcgatggcc acacgttgtt tttgaccacc tgataggtta
    56281 tctggattgg cattagcttt atctgctagt ccaacctttt caagcaactc cattcccaat
    56341 tcctcagctt cttccttagt catcaacttg tgctcaatag gagcaaaggt gatgttgtcc
    56401 aatacagaca tatgagggaa gaggttgaag tgttggaata ccatgccgat attttcacgg
    56461 acgtggtcaa cattggttgt tttttcagtt aaatcatagc cgttcacagt gatgtgaccg
    56521 ctagtgactt cttctaaaag attgaggcta cggaggaaag ttgacttacc agaacctgaa
    56581 ggaccgatga tacaaacaac atctccttca tagaacttag tcgtaattcc ttttaggact
    56641 tcattttttc catagtgctt gtgtaaatca tttacatcaa tttttaattt tgccattaac
    56701 gaatcctctt ttctaagcgt ttcgctagtc tagtcaaaag tgtgataatt acaagataga
    56761 agatagcaag gattgcatac atcttgaaac tttggtagtt acgagcaatg ataatcttac
    56821 cagtttggaa gagttcaacc aaaccgatag cagatacgat agttgtatct ttaagagcga
    56881 taacgaattg gttgacaaag tttggcaaca tcaatttagt tgcttgtggc aagataatct
    56941 tacgcatggt ttttccataa gagataccca agcttcggct ggcttccatt tggccaactg
    57001 gaacggcctg aataccacca cgaacgattt cagcgatata agcagccgca ttgagtgaga
    57061 gggcaatggt tccagctaca aagtcgttaa ttgggctttg ttggcctgtg atagactcga
    57121 tgaagtttgg aattccccag aagatgaagg ctgcaagaat catcaatgga ataccacgaa
    57181 taacgtcaac gaaaatctca gagatgacgc gaagagattt gtatgggcta acgctaaaca
    57241 taccgaagat aattccgatg acaatggcaa tagcaaatga gataagagct agagcaagag
    57301 tgataccaag accgctaagg agttgtttgt agttgttttg aagcaagccc cagagcgttg
    57361 tttcgtcaac agtacttgtt gaagcagttg aagattcgct agctaggtat ttgtcaagaa
    57421 tcttttggaa ttcaccgttt gctttaaggt ttgcaagtcc gttgttgaac atttcaatca
    57481 gttctggatt tgctcctttt ttaacggcaa aggctgtttc accgattgga gttccagaga
    57541 ttggagtttt caatttttga ccttggctga tagaatattt gagaacaggt tcatcatcca
    57601 taacggcatc aatggcacca gtgtttaaac tgtcatacat tgaagaacca tcagcaaagg
    57661 ttttgatttt gtaaccgtat ttgctttgat tttctgttag gaaggtttga gaagcagttc
    57721 cgtttttaac accgactgtc tttcctttta gatcttcata agaagcaata ttgcttgatt
    57781 ctttgacacc aagaatggta ttagcagtgt agtatgattc tgagaagtca aaagttgcct
    57841 tacgagcatc tgtgacagac ataccagcaa tgataccatc ggcttgacca gcttggacag
    57901 cactgatagc agcatcaaaa ccagggttgg tgatttcaat ttcaaaacct tggtctttag
    57961 cgattgcctt aatcaattcc atatcaatac cagtgtattg gttgcttgaa ttttggaaaa
    58021 caaaaggtgc aaaagaagaa tcgctggcaa tgatatattt agccttaaca ggaatagctt
    58081 ttaatcctgc gagagtagtt gtagttggca ctgctgaaga tgatgaagca gtccatttct
    58141 tgataatttt atcaagacta ccatcttttt tcatttcagc caaggcttgg ttaaattcag
    58201 taaccaggtg ctcgtattta cttccttttt tcacaccgaa agcaaaactt cctacagctt
    58261 caccatccat ttcaatatgg aggtcttgac cttggttaat ggcatattcg ataacaggtt
    58321 tgtcatccat catggcatcg atggcaccag cactcaagct gttgttcatt aaatcaccag
    58381 tgtcaaatgt tttaatagta aagccgtatt tatctttgat tgtttcaagg aaacgttgag
    58441 cggcagttcc gtttttaaca ccaacggttt tgccagttaa ttggtcgtac ttgctaattt
    58501 tgtgtgactt tgtagtagca atgacaactt ttgtatcata gtaagtatca gacatggtga
    58561 agactttttc acgttcttta gtctttgtca tccctgccat gatagcgtcg gcttgcccag
    58621 cttgaaccgc attgactgct gcgtcaaatc caggatagga catctgaatg ttccagcctt
    58681 taatctcagc gactttgtta ataatgtcaa catcaattcc tttataagtt tgatctgaat
    58741 ctttaaactc aaaaggtgca taggcggtat cagaaacaat cttaatcgtt tctgctttgg
    58801 caatacctaa tgagaaaatt gggaataaaa ttagcaaaaa tgctagaaat tttttcttca
    58861 tttttagtct ccttttccga atattctctc attatatcag aaaaagtaaa aaaaggcaaa
    58921 tttttatatt tttcgtgtca gaaaatataa cattgatttt tccttttctt tcttgaattg
    58981 ctattaaaaa ttgctataat aatactatat atagaagaaa agaggacggg gatatgaaga
    59041 acaaaagaat atttaaagac ttccaagctt caaaaatgaa tttaaacctt tacacaagcc
    59101 ccttgttagc ctttgttttt gtcttcatag gagagtttgt ggcttatact ttatatggta
    59161 ttagtttgtt agctctcatc ggacttgcta gaaattttgg agaggctggt caaaatcttg
    59221 caagctactt gcagaccttg catcagagct tgacggataa aacaagtgac tttcgtttaa
    59281 ttttaggatt actggccttt ggttttattc ttaacactgt gttcagatgg acaagaaaag
    59341 ttgagaaaag acctattcga accttgggat tttatagaga gaattgcctc agcaatcttc
    59401 tgaaaggatt tagtctaggc ctggcacttt ttcttctgac cttgttaagt ttagtggtct
    59461 taggtcaata tcgtttggaa tccattcact tgaatcctta ttatcttgcc tttgtcgtct
    59521 ttactatccc attttggatt ttacagggga cagcagaaga agtggtggcc cgtgcttggc
    59581 tacttcctca attggcctca agaaccaatc taaaactagc tattcttata tctagcctgt
    59641 tctttaccct gcttcatatg ggcaattctg gcctcacccc tctatctcta gtaaatctct
    59701 ttttattcgg agttgccatg gctctttacc ttctcaaaac tgatacagtt tggggtgttg
    59761 caggtattca tggtgcttgg aattttgctc agggtaatct ctttgggatt ttagttagtg
    59821 gtcaaccgtc aggaacgtct ctgatgacct ttttaccaca aggcaatcaa gattggctat
    59881 caggtggttc ttttggcata gaaggttcca ttatgacaag tctggtatta ctactgctga
    59941 ttgtctatct tgctaataaa ttaaagaaag aaaatgaaag gatgtgactt cggtccgtcc
    60001 ttttcttcgt gaaaatacta taagtatgct aaaataggaa tagcacatgg agagaggatt
    60061 cttatgatca atcacattac agataatcaa tttaaactag tatcaaaata tcaaccatca
    60121 ggagatcaac cccaagctat cgagcagttg gtggataaca ttgagggggg agaaaaagct
    60181 cagattctga tgggggcgac tggaacaggg aagacctata ctatgagtca ggtcatttct
    60241 aaagtcaata aaccaactct ggttattgcc cacaataaaa ctctggctgg tcagctctat
    60301 ggggagttta aggaattttt ccctgaaaat gcagttgagt atttcgtatc ctactatgat
    60361 tattaccagc cagaggccta tgtcccttct agcgatacct atattgagaa ggatagttct
    60421 gtcaatgacg agattgacaa gcttcgccac tcagctacct cagccctttt ggagcgtaat
    60481 gatgttatcg tcgtggcctc agtctcttgt atctatggtt tgggttcgcc caaggaatac
    60541 gctgatagtg tcgttagtct ccgtcctggt ctagagattt ctcgtgataa actcttgaat
    60601 gacttggtcg atattcagtt tgaacgcaat gatattgatt tccaacgcgg aagatttcgc
    60661 gttcgtgggg atgtggtaga gattttccca gcttcccgag atgaacatgc ctttcgagta
    60721 gaattttttg gagacgaaat tgaccgtatt cgtgaagttg aggctctgac aggtcaggtg
    60781 ttgggagaag tggatcattt agcgattttc ccagcgacac actttgtgac caatgacgac
    60841 cacatggaag ttgccattgc aaagattcag gccgagttgg aagaacaatt agctgtcttt
    60901 gaaaaggaag gtaaactgct tgaagcccag cgtttgaaac agcggacaga gtatgatatc
    60961 gaaatgttgc gtgagatggg ctataccaat ggggttgaaa attattctcg ccacatggat
    61021 ggacggagcg aaggagagcc tccttatacg cttctcgact tcttcccaga tgatttcttg
    61081 attatgattg acgagagtca tatgaccata gggcaaatca agggcatgta caatggagac
    61141 cgttcgcgta aagaaatgct ggttaattat ggtttccgtt tgccgtctgc tttggacaat
    61201 cgtcctctcc gtcgggagga gtttgagagt cacgttcatc agattgttta cgtttcagcg
    61261 acacctggtg actatgaaaa tgaacagacc gagacagtga ttgagcaaat cattcgtcca
    61321 acgggactct tggatccaga ggtggaagtc cgtccgacta tgggacagat tgatgacctc
    61381 ttgggtgaaa tcaatgcccg cgttgaaaaa aatgagcgta cctttatcac aactttgacc
    61441 aagaaaatgg cagaggactt gaccgactac ttcaaggaaa tgggtattaa ggtcaagtac
    61501 atgcactcgg atatcaagac cttggaacgg acggagatta tccgtgacct gcgcttgggt
    61561 gtctttgatg tcttggtcgg aattaacctg ctccgtgaag gaattgacgt tcctgaagtg
    61621 agcctcgtag ctattctcga tgctgacaag gaaggtttcc ttcgcaacga acgtggactc
    61681 atccagacta ttggacgtgc tgctcgtaac agtgaaggcc atgtcatcat gtatgcagac
    61741 acggttaccc agtctatgca acgtgctatc gatgaaactg cccgccgtcg gaaaattcag
    61801 atggcctata atgaagagca tggtatcgta ccacagacaa tcaagaaaga aatccgtgac
    61861 ctgattgccg tgaccaaggc agttgcgaag gaagaggaca aggaagtcga catcaatagc
    61921 ctcaacaaac aagagcgcaa agaactcgtc aagaaactag aaaaacaaat gcaagaagcc
    61981 gtcgaagtgc ttgactttga actggcggct cagatccgtg atatgatgtt ggaagtcaag
    62041 gccttggatt aggggaatag tatgatttat ttaagaaagt taaagagaga agatttgatg
    62101 tctttatggg aaatggctta ttcacaactt aatccagttt ggaaacagta tgatgctccc
    62161 tattatgatg attatcagta tttttcaaat tttaaagaat tcgaactaca aaaatcagaa
    62221 tccattttaa gcaactcaaa ttgccttggt atttttgttg atgataaact agttgggact
    62281 gtttcgcgtt attgggtatg taaacaaaca agatggatgg aattgggaat tggtatttat
    62341 gataaaaaat tctggaacac tggtattggg aaagttgcta tgttgcagtg gatagatagg
    62401 acgtttcagg attacttgga gttggagcat ctgggtttga caacttggtc aggaaatctt
    62461 ggtatgatga aacttgctga aaaattaaga atgaaaaaag aagctcatat tccaaaagtt
    62521 cgttattatc aaggtaaata ttttgatagt attaaatatg gtattttgag agaagactgg
    62581 gagaaaataa atgacggtta ttatcaaatc aatggaaact cctgaagaga tagaaggtaa
    62641 atccctcgtt cactggcaaa cgtggagaga ggcttatgat gaccttttgc ctgcagaatt
    62701 tcaggagaca atgacattag aaagatgtcg attctttagt caaaagtatc cagaaaatac
    62761 attgattgcg ataggtgatc tgaaggtagt tggttttatc agttatggta actttcgtga
    62821 tgagactatt caagctggtg aaattattgc tttatatgtt ttaaaagact attatggaaa
    62881 aggtatcgca caaaagttaa tgaaagcagc tttgactgct cttaatcatt tttctgaaat
    62941 tttcttatgg gtattgaaag ataacaagcg cgccattgct ttctatcaaa aaatgggttt
    63001 tacttttgat ggacaagaaa aaatacttga acttggaaag cctataaagg aaaaacgaat
    63061 ggtgttctat tctaaataat tttaaaaaag taaaagataa tatctctggt agtaagtctg
    63121 aaaatttaat aaattagaaa gcgagtaaat ttatgtcccg ttcccaatta acaattttaa
    63181 caaatatctg tctgattgaa gacctcgaaa ctcagcgcgt ggtgatgcag tatcgcgccc
    63241 ctgaaaacaa tcgctggtct ggttatgcct ttcctggagg tcatgtagaa aatgatgagg
    63301 cttttgcgga gtctgtcatt cgtgaaatct acgaagaaac agggttgact atccaaaatc
    63361 ctcaacttgt cggcattaaa aattggccac tagatacagg tgggcgctat attgtcattt
    63421 gttataaggc gactgagttc tctggtaccc ttcaatcttc agaagaggga gaagtttctt
    63481 gggtgcaaaa agaccagatt ccaaacttaa atctggccta tgatatgcta ccattgatgg
    63541 aaatgatgga agctcccgac aagtcagagt ttttctatcc tcgccgtaca gaagacgatt
    63601 gggaaaagaa aatcttctag tcttttacta aataacctag ctgatccaag gcctcctcga
    63661 tatagtggag gtcttgttgt gtttcggctt cgactaggtg gtaatggatg ccatctgtca
    63721 attcagatag aggtctaaag tcagaatgct cgacttgttc tagaaaatgt tgcacatctc
    63781 tgcgacaaga cagtttaagc aaggtttcaa tttctccata aacgggatgg tcaatcaagg
    63841 tattctggac atgcccacca ttatcgacaa tagcaaggag ttcctgaccg atttcttcaa
    63901 cttcatgttt cactttgaaa agtttgtgaa cataaggatt ggtatcaatt tgcttataga
    63961 tgtagccacg attggtggat aggataggag cgccatcggc tctcaaaatt gcaatgtcct
    64021 gcacaatgac ctgacgtgtg acatgaaaat gttccgccaa actttggcca ttaagggctt
    64081 taggagcttc tttcaagagt tggagcaggg cttgtttgcg atcttttgtc atagcttttc
    64141 cttttaacgg cgttttcgaa gcactttata gacagctagt gctaatgtat agtctaccat
    64201 actatggata attgtgccaa atccaactag tacaaataga acatagaaca tattttctac
    64261 attggtaccc gaagttgcgt aaaaaaggac acaggctaat acttcagcaa gggcatgaac
    64321 aacagccaaa acaaaattga aaacccagga agattttggt ttatctaggg tatcggggaa
    64381 tttttgtagg taaagagctc ctaaagcacc aaaagatata tgggaaaaag cccgaaaaac
    64441 gataaccatg ggatagccag ccatcaaaaa tccaaaacta gaggctagga tgacaaaaac
    64501 tgccatcaag ggcgacaaga acatggctat aaaaatagcg atgtggctcc ccaaagtata
    64561 ggaagcaggt ggaatgacaa tcttgaaagg cataacaatt ggaatcaaaa tcgcaatagc
    64621 cgttaaaagg gctgtcattg tcataaattg tgtctttttc cgtgtattca caagaatctc
    64681 ctttttaact gcatatacac tagtatggta caataaacca gacaataaag caagaattta
    64741 cttgggttta tagatcattt tttagttaaa agttatagta gatggaaact agaatagtcc
    64801 acctctactt ctaaaacatt gttagcgatt tgactgtcct gatcgatttg tcctgttctt
    64861 atttcgtttt actatagtaa agatttcatt aaaaagaaac tgtatagagc aaaatctcca
    64921 ccttcaggtt tgaaagcgga gattgttttt attttttcaa ggtttgtagt cgtggaacaa
    64981 tggctagggt tagaattgca gaaatgacca attcagcaat tgaatttgtt gagataacgg
    65041 ttgccagaag aagttggata tttccattat aaacatttcc aaataggaag aagattcctc
    65101 caaggacaaa gatagtattt gtcaaggaac caagggctcc agctaaaatc agaccagtct
    65161 tgtttttcat cagtttatag actaagtaag gagttaaacc aatcaaaata cgtgggacga
    65221 tggcaatgat agctgagtag atgtttccgt ttggtacgaa gggagagaag aggtagcttg
    65281 tcggtagaat cgtaatcgtg ttaaccgtca agctaagtaa tcccatcaaa aatccaagtg
    65341 taaccccaac tcgtggacca taaataatgc tggcaataat gacaggaata tgaacaatgg
    65401 tcggtttgat tggaaatgga aaaaggttaa agataagtga gctcagaaag tgtatcacga
    65461 gcatggtagc aaaaaagata gcaatgggtg caatattaga gcgttttttc atcgatagtt
    65521 tcctttattc tttctaaaat aattgtgagg tcagctaaag ctcctcgtcc gtggtctcca
    65581 caagctagta gggattcctt aggagcaatc agctgatagc cgtaggtttc taatgttttc
    65641 agattattct gagttactgg atggtcatac atttttgtat tcatagcagg agctattagt
    65701 ttgggaatat gacttggtag ggctagagct gtactggtta ccatgttgtc cgcaaatccg
    65761 tgagctagtt ttgcaatagt gttagcagtt gcaggtacca cgataaataa atctgctttt
    65821 tttccaagtt cgatatgatt gacctgatca ggatagggtt ccttcatgac atccaagtgg
    65881 acaggattct gtgagagtac ctgtagtgtc aaaggttgga taaactctgt agcagcctga
    65941 gtcattaaga cagtgacttg atggccttgt ttttttagag aactgactaa atctgccgac
    66001 ttataagagg cgattgaacc cgttacagcc aagagaatgt ttgccatagt tttcctttct
    66061 aagaatgata ggcttgaatt ttttcaagga ggagttctgc aatttcttct ttagtctgga
    66121 ctgtttgaag ctgatttttc tcaacaaata tagctcggtg ctgatctgct gaaatttgag
    66181 tcaggtcatt cgcgatgatt aaatctgctt gattcttgat aagacttttt cgtgcaatgt
    66241 caaccagatg atcttcggta acatcaacca gcagtttgaa accaatcaga tgaatagtgg
    66301 gattccattc cttgactagg gatatgattt tgggtgtctt tttaaggaac aaaacctgaa
    66361 cctcatcagt tgaagaaatc ttggcctgat gattctgctt gcttaaaaat tcttttagat
    66421 tggagctagc ctgaacttcc tcaagccctg tcatataaac aggagtgtag tcagaaacag
    66481 ccattgagtg gatcaagacc tgataatcct gaacacgttc ttgcatttct attagaaggt
    66541 ccttggtatt ggtaatttct cgaatactta ggttaggatg aggctctggc ttcagagctc
    66601 gttttgtcgt aattaaacaa acttcatgcc ctgcagaaag caaagtctct gtgataattt
    66661 tccccaagtg acctgtagaa tggttagtga tagagcggac gctatcgata gcttcactgg
    66721 taccgcccga tgtaactaaa attttcatag cactattgta cacaaaaata agcggtaagt
    66781 aaaatgaaag tgataaattt ttggtaaaaa cgaagattta ctatagtttc aaactataaa
    66841 gccatataaa atttaagaaa ggactctaaa agccttaaaa acagagatat ttacgaacgt
    66901 ttagagagtt ttaggatgtt aaaaatcttg ttttgtgata taatgaatta tcatataaga
    66961 ggttagagga attttgaatg aaaacagata ttgaaatcgc acagagtatt gagttgaagc
    67021 caattgttga tgttgtagag aaacttggta tttcttatga cgatttggag ttgtacggaa
    67081 agtacaaggc taaactcagc tttgataaaa ttcgtgcagt tgagagcaat ccagtcggta
    67141 aattgatctt ggttactgcc atcaacccaa cacctgcagg tgaaggaaag tcgacgctta
    67201 ccattggtct tgcggatgcc ttgaacaaga ttggcaagaa aaccatgatt gctatccgcg
    67261 aaccgtctct tggtccagtc atggggatca agggtggtgc tgctggtggt gggtatgcac
    67321 aagttctgcc aatggaagac atcaacctcc actttactgg agatatgcat gctattacaa
    67381 ctgccaacaa tgccctttct gccttgattg acaaccactt gcaccaaggg aatgagctgg
    67441 aaattgatca acgtcgtatc ctctggaaac gcgttgtgga cttgaacgac cgtgcgctcc
    67501 gccatgtgac tgttggtctt ggtggtcctc taaacggtat tccacgtgag gatggttttg
    67561 atattacagt tgcttcagaa atcatggcaa ttctttgctt ggcaacggac atcgaggact
    67621 tgaaacgtcg tttggcgaat atcgttattg gttatcgcta tgaccgtacg cctgtttctg
    67681 taggtgattt gcaggttgag ggtgccttgg ctttgatttt gaaggatgct attaagccaa
    67741 acttggttca gacaatttac ggtacacctg cctttgtaca cggtggtcca tttgccaata
    67801 tcgctcatgg ctgtaactct gttttggcga cgacaacagc ccttcacttg gctgattaca
    67861 ctgttactga agctggtttt ggtgcggacc ttggtgctga gaaattcctt gatatcaaga
    67921 caccaaactt gccaacatct ccagatgcag ttgtcattgt cgcaaccctt cgtgccctta
    67981 agatgaatgg tggtgtggct aaagacgctc tgactgaaga aaatgtagag gcagttcgtg
    68041 caggttttgc taacttgaaa cgccacgttg aaaatatccg taagttcggt attccagcag
    68101 ttgtagctat taacgaattt gtatctgata cagaagctga aattgcagtc ttaaaagaac
    68161 tctgtgcctc aatcgatgta ccagttgaat tggctagtgt ctgggctgat ggagcagaag
    68221 gtggagtagc acttgccgaa acagttgtta agacaattgc tgaaaatcca gctaactata
    68281 aacgtttgta cgataatgcc ctttctgtcc aagaaaagat tgaaaaaatt gtcactgaaa
    68341 tctatcgtgg tagcaaagtg aactttgaga agaaagctca aacacaaatt gctcaaatcg
    68401 ttcaaaacgg ttgggacaaa ttgccaatct gtatggctaa aactcaatac agtttctcag
    68461 acaatccaaa tgcgcttgga gcaccagaaa actttgaaat taccattcgt gaattggtac
    68521 caaaattagg tgcaggcttc atcgttgcct taactggtga tgttatgacc atgccaggtc
    68581 ttccaaaacg tccagcagct ctcaacatgg atgttgaaag cgatggaact gtactaggct
    68641 tgttctagtg gattgaaatt gactttaagt gagtttggaa ataatatcca agctcatttt
    68701 ttctcagact gcaagcagtt catcaataag atttatactc ttcgaaaatc tcttcaaact
    68761 acgtcagctt caccttgccg taggtatatg atactgactc tgtcagttct atccacaacc
    68821 tcaaaacagt gttttgagca acctgcggct agcttcctag tttgctcttt gattttcatt
    68881 gagtataagt agatagaaaa gttatatact atctattgaa aatattataa atcagataca
    68941 aagacaggtg aaaccttctg tgttatacta gagatatgtt agatttgaaa gaatacggta
    69001 tcgtcatgtg gccggaggag aaagtcattt ctttccgtga gaaacttctc gcttggtatg
    69061 atgaaaacaa aagagatttg ccttggagga gaagtaaaaa tccttatcac atctgggtat
    69121 ctgaaatcat gcttcagcag accagggtgg atacagttat cccttactac gaaagattcc
    69181 tggactggtt tccaactgtc gaaagtctgg caactgcgcc tgaggagagt ttactgaaag
    69241 cttgggaggg cttgggctat tattctcgag ttcgcaatat gcaggctgca gcccagcaga
    69301 ttatgactga ctttggtggc caatttccaa atacctatga aggaatttcc agcttgaaag
    69361 ggattggacc ttacacagca ggagccattt ccagtattgc ttttaacttg cctgagccag
    69421 ctgtagatgg taatgtcatg cgggtcttgg cgcgtctgtt tgaagtcaac cacgatattg
    69481 ggattccaag taatcgaaaa atttttcagg caatgatgga gatcttgatt aatccggatc
    69541 gtccgggtga ttttaatcaa gccttgatgg acttagggtc tgatattgag tctcctgtaa
    69601 atcccagacc tgaagaaagc ccagtcaagg actttagtgc ggcatatcag aatggaacca
    69661 tggaccgtta tccaattaaa tctcctaaga aaaagccggt tcctatttat cttaaagcat
    69721 tagttgtgaa aaattctcaa ggacaatttt tacttgaaaa aaatgaaagc gaaaagctct
    69781 tggcaggttt ttggcatttc ccccttatag aagttgataa cttttcgcaa gaagagcagt
    69841 ttgacctctt tcatcaggtt gcggaagaaa gtgtgaactt tggccccagt ccagaagaga
    69901 gtttccagca ggactatgac ctagatgttg attggcttga tgtttgtttt gatacagtcc
    69961 agcatgtttt tagtcatcga aaatggcatg tacagattgt agcaggtcag gtgagtgact
    70021 tccatgattt ttcagatagg gaagttcgtt ggctttcacc agaagaattc aagaattacc
    70081 cacttgccaa accccaacaa aaaatctggc aggcttatgc acaagccaac ttagacagta
    70141 gccaagacta gctattgtgt cttctgacta ttttttggta taatagtaga gaaaaaggtg
    70201 aacatatgaa aaaaatacta attgtagatg atgagaaacc aatctcggat attatcaagt
    70261 ttaatatgac caaggaaggt tacgaagttg taactgcttt taatggtcgt gaagcgctag
    70321 agcaatttga agcagagcaa ccagatatta ttattctgga tttgatgctt ccagaaattg
    70381 atggtttaga agttgctaag accattcgta agacaagcag tgtgcccatt cttatgcttt
    70441 cagccaaaga tagtgaattt gataaggtta tcggtttgga acttggggca gatgactatg
    70501 taacgaaacc cttctccaat cgtgagttgc aggcgcgtgt taaagctctt ctgcgtcgtt
    70561 ctcaacctat gccagtagat ggtcaggaag cagatagtaa acctcaacct atccaaattg
    70621 gggatttaga aattgttcca gacgcctacg tggctaaaaa atatggcgaa gaactagact
    70681 taacccatcg tgaatttgag cttttgtatc atttagcatc gcatacaggt caagtcatca
    70741 cgcgcgaaca cttgcttgag actgtctggg gttatgacta ttttggtgat gtccgtacag
    70801 ttgatgtgac tgtacgacgt ctgcgtgaga agattgaaga tacgcccagc cgaccagagt
    70861 atatcttgac gcgccgtggt gtagggtatt acatgagaaa taatgcttga tttactgaaa
    70921 caaaccattt ttaccagaga ttttatcttt atcctgattt tgttaggttt catccttgtt
    70981 gtgaccctct tattactgga aaatagacgt gataatattc agttgaagca aatcaatcaa
    71041 aaggttaaag atttgattgc aggagattat tccaaggttc ttgatatgca aggtgggtct
    71101 gaaatcacca atattaccaa taatttgaat gacttgtcgg aggttattcg tctcactcag
    71161 gaaaatctag aacaagagag taagaggcta aatagtattc tgttttatat gacagatggg
    71221 gttcttgcga ccaaccgtcg gggtcagatt atcatgatta acgatacagc caagaagcaa
    71281 ctggggttgg ttaaggaaga tgttctgaat agaagcattt tggaattgct caagatagaa
    71341 gaaaactatg aattgcgtga tttgattacc caaagtccag aattgttgct agattcccaa
    71401 gatatcaatg gcgaatattt gaaccttcga gttcgctttg ccttgatacg tcgagagtct
    71461 ggctttattt caggtttggt ggctgttttg catgatacga cggagcagga gaaggaagaa
    71521 cgcgaacgaa gactctttgt ttccaatgtt agccatgagt tacggactcc tctgactagc
    71581 gtaaaatcct atcttgaagc cttggatgag ggggctttgt gtgaaactgt agcaccagac
    71641 tttatcaagg tttctcttga tgagaccaac cgtatgatgc gcatggtgac ggatctcctc
    71701 catctttcac gtattgataa tgctaccagt cacctagatg tggaactgat taacttcact
    71761 gcttttatta cctttatcct caatcgtttt gacaagatga aaggacagga aaaggagaaa
    71821 aaatatgagt tggtgagaga ttatcccatc aattctatct ggatggaaat tgatacagat
    71881 aagatgacgc aggttgtcga caatatttta aataatgcta ttaagtattc gccagatggg
    71941 ggtaaaatca ctgtcagaat gaagacaact gaagaccaga tgattttatc catttctgac
    72001 cacggtttgg ggattcctaa gcaggattta ccacgtatct ttgaccgttt ctatcgtgtg
    72061 gatcgtgcta gaagtcgtgc acaaggtggt acaggtctag gactgtctat cgctaaagaa
    72121 attatcaaac aacataaggg ctttatttgg gccaagagtg aatacggcaa gggttcaacc
    72181 tttaccattg tgctccctta tgataaggat gcagtgaaag aagaagtatg ggaggatgaa
    72241 gtagaagact agaatgagtg aaataggctt taaatacagt attttagcgt cgggttccag
    72301 tggaaattct ttttatctgg aaacctcaaa aaagaagctt ttagtagatg caggcttgtc
    72361 tggcaagaaa attaccagtc tgctagctga aattaaccgt aagccagaag acctggatgc
    72421 catcttgatt acccatgagc attcagatca tatccatgga gtaggcgttt tggctcgcaa
    72481 gtatggtatg gatctttatg ccaatgaaaa gacctggcaa gctatggaaa atagtaaata
    72541 tcttggcaag gtggattctt cgcaaaagca tatttttgaa atgggtaaaa ccaaaacctt
    72601 tggagatatc gacatcgaga gttttggtgt aagccatgat gcagtcgcac cgcagttcta
    72661 tcgctttatg aaggatgata agagttttgt cctcttgaca gatacaggtt atgtcagtga
    72721 ccgtatggcg ggcattgtcg aaaatgcgga tggttatctt atcgaggcca accatgatgt
    72781 agagattttg cgatcaggtt cttacgcttg gcgcctcaaa caacgaatcc tatctgacct
    72841 tggtcacctt tctaacgagg acggtgctga agctatgatt cggacgctag gaaatcgtac
    72901 taagaagatt taccttgggc atttatctaa ggaaaacaat atcaaggaac tggctcatat
    72961 gaccatggtc aatcagctgg ctcaagctga tctgggagtc ggagtagact ttaaggttta
    73021 tgatacctca ccagataccg caacaccatt gacagagata tagaaagaac gctgagaagg
    73081 tgttcttttt atattgactg gacacctaaa aagtaataca atggtgttac cattaaaaaa
    73141 gggagcacaa aagatgacta ctattacatt aaaagtttct gaagctgata aaacatttat
    73201 gaaagcaatg gctaagtttg aaggagtttc cctttcggaa cttattcgca ccaaaactct
    73261 tgaagcccta gaagatgaat acgatgctcg tgtggcagat ttagcctatc aagagtattt
    73321 agaagacttg gaaaaaggag ttgaacccat tacttgggaa gaaatgatgc atgatttagg
    73381 cttgaaggat gaataatttg tataaattag ttccaacaag acgttttatc aagcaattga
    73441 aaaaattgga ccgttatacg cagaagctaa ttacaaacta tttacaaacc aatgttttgg
    73501 aagatccaag acgacacgga aaggctttgg ttggtaatcg cgttggtcaa tggcgctata
    73561 gaattggtaa ttatcgagtt atcgtacaaa ttgtagatga tgaattagtt gttgctactc
    73621 tagaagttgg tcatcggaga gatatttatt aaattaaagc tgagtaataa cagtatagaa
    73681 agtaaggaga gtaaaaattt gaagaaaaaa tggttttttg cagattatta tgacacaaca
    73741 attattttac ttgctttaat ctctgttatt ctggtattac ttggatttgc tgagatgatt
    73801 gatttggaca atccgcctta tagtattatt gatttagtaa tctggggtgt ttttgtaatt
    73861 gattatagtt ggcgcttttt tacgactaaa agaaaatggc gctttattct tgaaaatatt
    73921 tttgatttat tggctattct ccctttaaat gctattttta cagtgtttcg attagggcgt
    73981 atctttcgtt tagctaaact aacaaaatta ctgaaactga ctcgtttact tagaataatt
    74041 ggacttacag gtaaattaga aagaaaaatt agtagattct tacggacaaa tggcctgatt
    74101 tatatcttgt atgttaatat ctttattgtt ctagtaggga gttcgatttt atccgttgtt
    74161 gaagaaaaat ccttctcaga tagtctttgg tgggctcttg tgacagtaac tactgttgga
    74221 tatggggata ttgttcctgc ttccattttt gggaaatggc tagcagtttt actgatgctt
    74281 gtcggtattg gaacaatagg gatgttaaca agtgctttga cgaacttttt tgtaaaggat
    74341 aatccagatg aacagataaa acttgataaa ctcaaagatg aattatcgag ccaaagaata
    74401 ttactagaga aacaatctaa aaagatagaa gaactacata aaatgataca agatttgatt
    74461 gaaaaaacat aataaaaata aagatgagac aaagtttgag ctgaggagaa atcaagtaag
    74521 ttttgacaca atagaacgca tattatcagg tataaaaact tgatactatg cgttttatag
    74581 agaaataaaa taaaatctga acaaatcaat taggaattta aagcatttta taacaacgtt
    74641 ttagagtgat ggggtgctat ttcaacttca acctactata atacagaaaa aaacaactcc
    74701 ctgatgattc aaggagttgt ctatagttaa attagttttt agaagcttct tggaattctg
    74761 gatttttcca tgcttcgtca ataatagctt gcaattcttt agcagatgct tgcatttttt
    74821 gagtttctgc atcgttcaat ggaatgttta ctggacgaac gataccatgt gcaccaacaa
    74881 cagctggttg accgataaag acattctcaa ctccgtatag accttcttgg aatactgaaa
    74941 gtggaagtac tgcgttttca tcgtcaagga ttgctttagt gatacgagca agggctactg
    75001 cgataccgta gtatgttgca ccttttttgt tgatgattgt gtaggctgca tcacgaacac
    75061 cttcgaacaa ttcaatcaat tcagcttctt gaacattttg agtgtcttta aggaattctt
    75121 caaggtttac accagcgatg ttagcgtgtg accaaacagc gaactcagag tcaccgtgtt
    75181 cacccatgat gtaggcgtgc actgaacgag catccacatc caatttttca gcaagtgctt
    75241 gacggaaacg agctgagtca agtgaagtac ctgaaccgat aacgcgttct ttagggaaac
    75301 cagagaattt ccaagttgag taagtcaaaa cgtcaactgg gttagcagca acaaggaaga
    75361 tacctttgaa accagattca acaacttgag ttacgattga tttgttgata gcaaggtttt
    75421 tacctacaag gtcaagacga gtttcacctg gtttttgagg tgcacctgca gtaatcacaa
    75481 caaggtcagc gtctgcacag tcagagtatt gagctgcata gattttttta ggtgaagtga
    75541 aggcaagggc gtgactaagg tcaagcgcat caccaacagc tttttcatgc aattgtggaa
    75601 tttcgataat tccaagctct tgtgcaattc cttggttaac aagtgcaaaa gcgtaagatg
    75661 aacctacagc accatcaccg acaaggataa cttttttgtg ttgtttagtt gaagtcattg
    75721 ttttaaacat ctccttaatt ttattagggg attttcccta gacaacttca ttctatcact
    75781 tttaaaaaac tttgtcacga atatgcctta tagttctcga tgtaaacgtt ttagtggttt
    75841 agaggctgaa atagatggga atttatggta taatgttgtt acttactaat tgtgaaatga
    75901 ggcatttatt aatgcaggat aaaaatttag tgaatgtcaa tctgacaaag gagatgaagg
    75961 caagttttat cgactacgcc atgagtgtta tcgtagcgcg agctcttcct gatgttcgag
    76021 atggcttaaa acctgttcac cgtcgcattc tctacggaat gaatgaattg ggtgtgaccc
    76081 cagacaaacc ccataaaaaa tctgctcgta ttacagggga tgtcatgggt aaatatcacc
    76141 cacacgggga ttcctctatt tatgaagcca tggtccgtat ggctcaatgg tggagctacc
    76201 gttacatgct tgtagatggt catgggaatt ttggttccat ggatggagat agtgctgccg
    76261 ctcaacgtta taccgaggca cgtatgagca agattgctct ggaaatgctt cgtgatatca
    76321 acaaaaatac agttgatttc gttgataact atgatgccaa tgaacgggaa cccttggtct
    76381 tgccagcgcg ttttccaaac cttttggtta atggagcaac tggtatcgcg gttgggatgg
    76441 caaccaatat tccacctcat aatctgggtg aaaccattga tgcagtgaag ttggtcatgg
    76501 ataatcctga agtgactacc aaggacttga tggaagtctt gcctggacca gattttccaa
    76561 ctggtgctct tgtcatgggg aaatcaggta tccataaggc ttatgaaaca ggtaaaggtt
    76621 cgattgccct acgttctcgt acagagattg aaacgactaa gactggtcgt gagcgcatcg
    76681 ttgtaacaga atttccttac atggtcaata aaaccaaggt gcatgagcat attgttcgct
    76741 tggttcagga aaaacgcatt gagggtatca cagcagtacg tgatgagtca aaccgtgaag
    76801 gtgttcgatt tgttattgaa gtcaagcgcg acgcctcagc caatgttatt ctcaataacc
    76861 tcttcaaaat gacccaaatg caaaccaatt ttggtttcaa tatgctcgct atccaaaatg
    76921 gtataccgaa aattttgtct cttcgtcaga ttttggatgc ttatatcgag caccaaaaag
    76981 aagtggttgt tcgtcgtaca cgttttgata aggaaaaagc ggaagcgcgc gctcatatct
    77041 tagaaggtct cttgattgcg ctagaccata tcgacgaagt gattcgtatc atccgtgcta
    77101 gtgaaacgga tgcggaagct caagctgagt tgatgagcaa gtttaagctt tctgaacgtc
    77161 aaagtcaagc tatccttgat atgcgtcttc gtcgtttgac aggtttggaa cgcgataaga
    77221 ttcaatctga gtatgatgac ctcttggctc tgattgcgga tttagcagat attcttgcta
    77281 agcctgaacg tgtttctcaa attatcaaag acgaattgga tgaagttaaa cgtaaatttt
    77341 ctgataagcg ccgtacagag ttgatgattg gacaggtctt gagtctcgag gatgaggact
    77401 tgattgaaga atcggatgtc ttgattaccc tttctaacag aggctacatt aagcgtttgg
    77461 atcaggacga gttcactgct caaaaacgtg ggggtcgtgg tgtccaagga acgggagtga
    77521 aagatgatga ctttgttcgt gagttagtgt caactagcac ccatgatcat ctgctcttct
    77581 tcacaaacaa gggacgtgtc tatcgtctta aaggttatga aattcctgag tatggtcgga
    77641 ctgccaaagg gctaccagta gtcaatctct tgaaattgga tgaagacgaa agtattcaga
    77701 cggttatcaa tgttgagtct gatcgcagtg atgatgctta tctcttcttt acaacccgtc
    77761 acggtattgt gaagagaacc agtgttaagg agtttgccaa tattcgtcaa aatggtctca
    77821 aagcgctgaa tttaaaggat gaagatgagt taatcaatgt cttgttggca gaaggagata
    77881 tggatattat cattggtacc aagtttggtt atgcagttcg ctttaatcaa tcagccgttc
    77941 gtggtatgag tcgtatcgcc actggtgtga aaggtgttaa ccttcgtgaa ggagacacag
    78001 ttgttggtgc cagcttgatt actgatcaag atgaggttct tattatcaca gaaaaaggat
    78061 atggtaagcg tacagtcgct actgaatacc caacaaaagg tcgtggtggt aagggaatgc
    78121 agacagctaa aattaccgaa aaaaatggct tgctggccgg tcttatgact gttcaagggg
    78181 atgaggattt gatgattatc actgatacag gtgtcatgat tcgaaccaat cttgccaata
    78241 tttcacaaac aggacgtgca actatgggag ttaaagtaat gcgcctggat caagatgctc
    78301 agatagtgac tttcacaacg gttgcggtgg cagaaaaaga agaagttggg acagaaaacg
    78361 aaacagaagg tgaagaataa tgtctcaaaa aaataataaa aagaaaaaca agcgaaaaaa
    78421 tctgctgaca aatatcctag caggatttct gatattactg tcactggctt tgatttttaa
    78481 tactcaaatt cgaaatattt tcatagtctg gaataccaat aagtatcaag ttagccaggt
    78541 atcaaaagaa aaattagaag aaaatcagga tacagaaggc aattttgact ttgattctgt
    78601 caaagctatc tcttcggaag ctgttctaac ttctcaatgg gatgctcaaa aattaccagt
    78661 tattggggga attgcaattc ctgaattgga aatgaatttg ccgattttta aaggacttga
    78721 taatgttaat ctcttctacg gagctggtac aatgaaacgc gagcaagtaa tgggagaagg
    78781 aaattatagt ctagctagtc accatatctt tggtgttgat aatgctaata aaatgttatt
    78841 ttctccttta gataatgcta aaaatggcat gaagatttat ctaaccgata aaaataaagt
    78901 ttatacttat gaaatacgtg aagtcaaacg tgtgacaccg gatcgtgttg atgaagttga
    78961 tgatagagat ggggtcaatg aaatcacatt agtaacctgt gaagaccttg ctgctacaga
    79021 acgtattatt gtcaaaggtg atttgaaaga aacaaaagat tattcacaaa catctgatga
    79081 aattctaaca gctttcaatc aaccatataa acaattttat taatacaaat cagtgaaatc
    79141 atggatttca ctgatttttt aagattttca taaaaatata gtaaaatgaa ataagaatag
    79201 gacgaatcg
//
LOCUS       DAVBGY010000003        64160 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVBGY010000003 DAVBGY010000000
VERSION     DAVBGY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 64160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64160
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.003"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(136..483)
                     /locus_tag="VQ620_000172"
     CDS             complement(136..483)
                     /locus_tag="VQ620_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2762871.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(594..2369)
                     /gene="spxB"
                     /locus_tag="VQ620_000173"
     CDS             complement(594..2369)
                     /gene="spxB"
                     /locus_tag="VQ620_000173"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HET2762872.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(2576..4819)
                     /locus_tag="VQ620_000174"
     CDS             complement(2576..4819)
                     /locus_tag="VQ620_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET2762873.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYIVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQSLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(4829..5200)
                     /locus_tag="VQ620_000175"
     CDS             complement(4829..5200)
                     /locus_tag="VQ620_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET2762874.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QVGEFGFACGMNMMHGKMIVE"
     gene            complement(5211..5606)
                     /locus_tag="VQ620_000176"
     CDS             complement(5211..5606)
                     /locus_tag="VQ620_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HET2762875.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTIQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      5790..5888
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5972..6763
                     /gene="thiD"
                     /locus_tag="VQ620_000177"
     CDS             5972..6763
                     /gene="thiD"
                     /locus_tag="VQ620_000177"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET2762876.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEGMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(6972..7604)
                     /locus_tag="VQ620_000178"
     CDS             complement(6972..7604)
                     /locus_tag="VQ620_000178"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET2762877.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(7597..8400)
                     /locus_tag="VQ620_000179"
     CDS             complement(7597..8400)
                     /locus_tag="VQ620_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET2762878.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCTKLDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(8400..8924)
                     /gene="thiW"
                     /locus_tag="VQ620_000180"
     CDS             complement(8400..8924)
                     /gene="thiW"
                     /locus_tag="VQ620_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HET2762879.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERID"
     gene            complement(8929..9621)
                     /gene="tenA"
                     /locus_tag="VQ620_000181"
     CDS             complement(8929..9621)
                     /gene="tenA"
                     /locus_tag="VQ620_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HET2762880.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(9632..10282)
                     /locus_tag="VQ620_000182"
     CDS             complement(9632..10282)
                     /locus_tag="VQ620_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HET2762881.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQKLHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10284..11669)
                     /locus_tag="VQ620_000183"
     CDS             complement(10284..11669)
                     /locus_tag="VQ620_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2762882.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIVERVQTVGILLQNPNHQFTMESLFEELVFTMENI
                     GYHLQEIDSKIAEVVQQCRCKDILHRLIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELVYDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGSITWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(11670..12230)
                     /locus_tag="VQ620_000184"
     CDS             complement(11670..12230)
                     /locus_tag="VQ620_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2762883.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGFVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(12492..12580)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(12789..13418)
                     /locus_tag="VQ620_000185"
     CDS             complement(12789..13418)
                     /locus_tag="VQ620_000185"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET2762884.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(13420..14202)
                     /gene="thiM"
                     /locus_tag="VQ620_000186"
     CDS             complement(13420..14202)
                     /gene="thiM"
                     /locus_tag="VQ620_000186"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET2762885.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVMIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(14445..15113)
                     /locus_tag="VQ620_000187"
     CDS             complement(14445..15113)
                     /locus_tag="VQ620_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HET2762886.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVDKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(15328..15416)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15670..16806)
                     /gene="lctO"
                     /locus_tag="VQ620_000188"
     CDS             complement(15670..16806)
                     /gene="lctO"
                     /locus_tag="VQ620_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HET2762887.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(17431..18921)
                     /gene="lysS"
                     /locus_tag="VQ620_000189"
     CDS             complement(17431..18921)
                     /gene="lysS"
                     /locus_tag="VQ620_000189"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET2762888.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKTIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(19049..19195)
                     /locus_tag="VQ620_000190"
     CDS             complement(19049..19195)
                     /locus_tag="VQ620_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HET2762889.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAGDTF
                     TSFQ"
     gene            19421..20089
                     /locus_tag="VQ620_000191"
     CDS             19421..20089
                     /locus_tag="VQ620_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2762890.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            20049..20726
                     /locus_tag="VQ620_000192"
     CDS             20049..20726
                     /locus_tag="VQ620_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2762891.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIEISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLDFTRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GAAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            20727..21484
                     /locus_tag="VQ620_000193"
                     /pseudo
     CDS             20727..21484
                     /locus_tag="VQ620_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            21496..22357
                     /locus_tag="VQ620_000194"
                     /pseudo
     CDS             21496..22357
                     /locus_tag="VQ620_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(22437..23084)
                     /locus_tag="VQ620_000195"
     CDS             complement(22437..23084)
                     /locus_tag="VQ620_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2762892.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(23081..23779)
                     /locus_tag="VQ620_000196"
     CDS             complement(23081..23779)
                     /locus_tag="VQ620_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2762893.1"
                     /translation="MGKLSSHMWRLNKIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDVGGDLFLQLLFLVFLFQ
                     SIFYFTRQKRRFIE"
     gene            complement(23789..24541)
                     /locus_tag="VQ620_000197"
     CDS             complement(23789..24541)
                     /locus_tag="VQ620_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2762894.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(24541..25305)
                     /locus_tag="VQ620_000198"
     CDS             complement(24541..25305)
                     /locus_tag="VQ620_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2762895.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(25401..25804)
                     /locus_tag="VQ620_000199"
                     /pseudo
     CDS             complement(25401..25804)
                     /locus_tag="VQ620_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25984..26616)
                     /locus_tag="VQ620_000200"
     CDS             complement(25984..26616)
                     /locus_tag="VQ620_000200"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET2762896.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYRELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(26650..27351)
                     /gene="pyrF"
                     /locus_tag="VQ620_000201"
     CDS             complement(26650..27351)
                     /gene="pyrF"
                     /locus_tag="VQ620_000201"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET2762897.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQNWN"
     gene            27672..27764
                     /locus_tag="VQ620_000202"
                     /pseudo
     CDS             27672..27764
                     /locus_tag="VQ620_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(28086..28799)
                     /locus_tag="VQ620_000203"
     CDS             complement(28086..28799)
                     /locus_tag="VQ620_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2762898.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNKTLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(28792..29487)
                     /locus_tag="VQ620_000204"
     CDS             complement(28792..29487)
                     /locus_tag="VQ620_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2762899.1"
                     /translation="MTGQFLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYEKMS
                     LYNNLLFYSKFYNISKSRVDNLLKRVGLYDSRKMVAGKLSTGMRQRMLLARALINKPA
                     VLFLDEPTSGLDPTTSRTIHELILELKTAGTTIFLTTHDMNEATLLCDYVALLNKGKL
                     VEQGAPSELIQRYNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLE
                     DIFITLTGGKLNA"
     gene            complement(29827..31159)
                     /locus_tag="VQ620_000205"
                     /pseudo
     CDS             complement(29827..31159)
                     /locus_tag="VQ620_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812020.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
     gene            complement(31143..31941)
                     /locus_tag="VQ620_000206"
                     /pseudo
     CDS             complement(31143..31941)
                     /locus_tag="VQ620_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50 family protein"
     gene            complement(32731..33930)
                     /gene="divIB"
                     /locus_tag="VQ620_000207"
     CDS             complement(32731..33930)
                     /gene="divIB"
                     /locus_tag="VQ620_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HET2762900.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLSKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            complement(33940..34998)
                     /locus_tag="VQ620_000208"
     CDS             complement(33940..34998)
                     /locus_tag="VQ620_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HET2762901.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVCWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VAKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQLENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(35000..36352)
                     /locus_tag="VQ620_000209"
     CDS             complement(35000..36352)
                     /locus_tag="VQ620_000209"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET2762902.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(36456..37097)
                     /locus_tag="VQ620_000210"
     CDS             complement(36456..37097)
                     /locus_tag="VQ620_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2762903.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTFL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTTSLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(37099..39120)
                     /locus_tag="VQ620_000211"
     CDS             complement(37099..39120)
                     /locus_tag="VQ620_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2762904.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLMSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(39191..39490)
                     /locus_tag="VQ620_000212"
     CDS             complement(39191..39490)
                     /locus_tag="VQ620_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2762905.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSLVSRWNSHSDKGEASAGKTSYAWIWTKWGEQVAFYCDYD"
     gene            complement(39856..40110)
                     /locus_tag="VQ620_000213"
     CDS             complement(39856..40110)
                     /locus_tag="VQ620_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HET2762906.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(40130..41971)
                     /gene="typA"
                     /locus_tag="VQ620_000214"
     CDS             complement(40130..41971)
                     /gene="typA"
                     /locus_tag="VQ620_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET2762907.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            42133..42858
                     /locus_tag="VQ620_000215"
     CDS             42133..42858
                     /locus_tag="VQ620_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HET2762908.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVQVNELLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            complement(42898..43125)
                     /locus_tag="VQ620_000216"
     CDS             complement(42898..43125)
                     /locus_tag="VQ620_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2762909.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(43214..43594)
                     /locus_tag="VQ620_000217"
     CDS             complement(43214..43594)
                     /locus_tag="VQ620_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2762910.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            complement(43594..43863)
                     /locus_tag="VQ620_000218"
     CDS             complement(43594..43863)
                     /locus_tag="VQ620_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HET2762911.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            43927..44835
                     /locus_tag="VQ620_000219"
     CDS             43927..44835
                     /locus_tag="VQ620_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET2762912.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDN
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            complement(44889..45644)
                     /locus_tag="VQ620_000220"
     CDS             complement(44889..45644)
                     /locus_tag="VQ620_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET2762913.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(45665..46594)
                     /locus_tag="VQ620_000221"
     CDS             complement(45665..46594)
                     /locus_tag="VQ620_000221"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET2762914.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            complement(46609..47232)
                     /locus_tag="VQ620_000222"
     CDS             complement(46609..47232)
                     /locus_tag="VQ620_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HET2762915.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            complement(47225..48463)
                     /gene="hflX"
                     /locus_tag="VQ620_000223"
     CDS             complement(47225..48463)
                     /gene="hflX"
                     /locus_tag="VQ620_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET2762916.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            complement(48456..49340)
                     /gene="miaA"
                     /locus_tag="VQ620_000224"
     CDS             complement(48456..49340)
                     /gene="miaA"
                     /locus_tag="VQ620_000224"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET2762917.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKCFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            49461..49631
                     /locus_tag="VQ620_000225"
     CDS             49461..49631
                     /locus_tag="VQ620_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HET2762918.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            complement(49679..50518)
                     /locus_tag="VQ620_000226"
     CDS             complement(49679..50518)
                     /locus_tag="VQ620_000226"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET2762919.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPIITLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(50615..51574)
                     /locus_tag="VQ620_000227"
     CDS             complement(50615..51574)
                     /locus_tag="VQ620_000227"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HET2762920.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENTFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            complement(51701..52699)
                     /locus_tag="VQ620_000228"
     CDS             complement(51701..52699)
                     /locus_tag="VQ620_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2762921.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DLHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQAATTQTDTTSSTASGWVKQDGS
                     WYYFDGNGNLVKNAWQGNYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTA
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDG"
     gene            complement(52749..53366)
                     /locus_tag="VQ620_000229"
     CDS             complement(52749..53366)
                     /locus_tag="VQ620_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2762922.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            complement(53367..55088)
                     /gene="pabB"
                     /locus_tag="VQ620_000230"
     CDS             complement(53367..55088)
                     /gene="pabB"
                     /locus_tag="VQ620_000230"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HET2762923.1"
                     /translation="MHRKTVIDFRTLGERYTFAQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            complement(55157..60844)
                     /locus_tag="VQ620_000231"
     CDS             complement(55157..60844)
                     /locus_tag="VQ620_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2762924.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEETPKEEAPRTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKSEEKVAVKPESQPSDKPAEES
                     KVEPPVEQAKVPEQPVQPTQAEQPSTPKESSQEDNPKEDRGAEETPKQEESTPDTKAE
                     ETVEPKEETKTAKGTQEEDYVGEALVPEQLPEYKVTEGTLVEESTTELDYKTETTEDP
                     TKYTDEETVLRNGEKGSQVTKTTYKTIGGVKTDQVLSTSTEVTKEPVNQQVIRGTKPI
                     EGTTVETREEIIPFETKEQEDDTLKRGTRQVTQEGVNDKKQITETYKTIRGEKTSDAP
                     TITETVIEKPQDKIIKNGTKELEKPTLEWAKTDKDVLKKSATASYTLTKPAGVEIKSI
                     KVALKDNTGTVVKEVTVEENNLQATLDNLKYYQGYTLSTTMVYDRGNGEETEILEDKE
                     VQLDLKKVEIKDIKETRLMKVDENGNETDSSLLETVPENLTSYYLKITTNHNKVTRLA
                     ITNIEEVTEEETSLFKVTASAPDLIQRDSNNSLSQTYTYYIEKPKASQNNVYYNFKDL
                     VDAMQKNPNGEFKLGSDLNATNVSTPSKSYVTGKFTGHLTSVDGKHFSIHNTARPLFD
                     RVEGGSIKNINLINVNINMPWANQLASLAQTVKNATVENIKVTGTIVGNNNVAGILDK
                     LDDAGIMTNVAFIGKVQSVGDKGWNLAGIASEIYRGNLNKAYADVELKGLNTRAGAIV
                     GHLAHDFNPRDVGAWSNVRNSVAKGTIDVARGDVGGLIGKDWPWGTIDNNISLVNVKR
                     GDIFFGSNDMDDEFVSNWIKPNNATVIGVSSGDKTHKQSSKIKEISQVEADKKIKDMG
                     ITADTFEVTEHLVDKLNRRILRDEEYKTIQDYNANRELVYRNVEKLQPFYNKEWIVNQ
                     GNKLTTDSPLMNKEVLSVTAMKGNDFITDLTDADHIMVHYADKTKDIFTISPKDSQVK
                     QVKEYSVAELGEVVYTPNMVVKDRADLISAIEGILSPIELQSDPIYQRLGRTGDNRVN
                     AIKNLYLEESFKEVKDHLNTFVTKLVENEDHQLNTDEAAKRALIKKIEDNKAAVLLGL
                     TYLNRYYGVKFDNFNIKELMLFKPDFYGKNVSVLDRLIEIGSKEDYIKGSRTQESFRE
                     VVAKNTLSGNLHDFLTYNMKLFTNETDINVWFKKAIENNAYVVEQPSTNPAFANKKYR
                     LYEGLNNGQHGRMILSLLNLKDAHLFMISTYNTISFSSFEKYGKDTEEKRESFKSEIN
                     KRAKEQVNYLDFWSRLATDNVRDKLLKSQNVVPTPVWDNHGAPGGWPDRFGHRKDKPD
                     YAPIREFFGRIDKYHDYKYGYGAYAYIFADPQPMDAVYFVMSDLISEYGTSAFTHETT
                     HVNDRMAYLGGHRHRQGTDLEAFAQGMLQTPAEHGHQGEYGALGLNMAFERSNDGNQW
                     YNPDPTKLQTRDQIDHYMKNYNEAMMMLDYAEAEAVLPKVKGDNSKWFKKIDRETRRP
                     MDRNKLSGPHQWDKVRDLIDAEKTTNLETIDDLVNNNFMTIHGNPGNKVFHPEDFGTA
                     YVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGYYGYEHGFIDYVSSKHQGAANKEN
                     NGLLGDDFIIKKVSNNQFSNLEDWKKQWYGEVLTKSKKGFAPIEINGTQISTYEQLKT
                     MFDEAVTKDLTELNDSSIKDKYRNTVVLKSKVFKALLKNTDGFSGDLFTAPQA"
     gene            complement(60866..61429)
                     /locus_tag="VQ620_000232"
     CDS             complement(60866..61429)
                     /locus_tag="VQ620_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HET2762925.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGVLFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            complement(61545..63236)
                     /locus_tag="VQ620_000233"
     CDS             complement(61545..63236)
                     /locus_tag="VQ620_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2762926.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            complement(63233..63970)
                     /locus_tag="VQ620_000234"
     CDS             complement(63233..63970)
                     /locus_tag="VQ620_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2762927.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
BASE COUNT        19313 a        13917 c        11317 g        19613 t
ORIGIN      
        1 gctttttgaa agcagatgag tattatatgc aatttcttta tataaaaaga ccggattgct
       61 ccgatctttc aatagttcat attctcaatt tctattttaa aaatagctaa ggttaacgtc
      121 aaatgactac gcgacctatt tcatacgata aaaatcaagc actagaccag caggtccttg
      181 aactaataag gactctgttc cccaatcggt tacagttggt ccgtgtaaaa cctttatacc
      241 aagctcattc aaccgtttgt agttctggtc tacatcctca acctcgatat gaataatgat
      301 tcctgactga aagttttcca aaggaaccaa atgattttgt gacaacataa ggcagtgact
      361 accaatcgta aactgagcaa aaccatcatc agcataatct gcctttttat ccaaaatatg
      421 ctccaagtca gcacagactt ggggaacatt tgaaacgata atatctaatt gatttaaatt
      481 catttactct cctccataaa aagaccgaat tgctccgatc ttttaaagtt ctgctctatg
      541 aaaatcaaag aataaagtct acaagtttca tatttgattt tcggcgagag gaattattta
      601 attgcgcgtg attgcaatcc ttcttcttcc aagaagagac ggaatggtac gagttcttct
      661 gcttcgtatt tttccttgaa ggctttgata gcttcttctg agtgaagttt tggatccaat
      721 tcaagtactt ctactggaag tggacggtgt tgagtgatgc gagcatcgat gacaacagtt
      781 ttaccttctt tgttcaattt aacagcttct gcaacaactg catcgatgtc ttcgatacgg
      841 tcaactgtga atccaacagc tccttgagct tccgcaattt tagcgtagtc agcgtttgtg
      901 aagtctacac caaacaagtg tttgtttgta tcttcgtatt tgttcttgat gaagccgtac
      961 tcagcatttg agaagacaag gttgataact ggaaggtcgt attgaacgtt tgtgataacg
     1021 tctgggtagc acatgttgaa tgctccatca cccatgatgt tccatacttg gcgatctgga
     1081 ttgtctttct tagcagcgat accaccagga agggcaatac ccattgtcgc aaagagcgga
     1141 gatgtacgcc acatattctt aggtgtcatg tgaaggtgac gagtagatgt ttgagtagtg
     1201 ttacctacgt cgattgagta gatagcgtct tgatcagcat gtttgttgat tgcattgtaa
     1261 acttgataca attgcaattc accctcagtt ttaccttcga gtttgttcat gtaatcacgc
     1321 cagttttggt tgttcttaac gtttgcacgc caccatggag ttgattcaac tgggtttact
     1381 ttgtcaagga tagctttagc tgcttgacca gcatcaccaa ggattgaagc gtcaagggca
     1441 tgacgtttac caagtttgta agggtcgata tcgacttgga tgaatttttc agtgttcttg
     1501 aatgcttcgt aaacttcagc aaatgggaag tttgaaccaa ggaaaagaac tgtgtctgct
     1561 tcaaagacca cctcgttggc tggtttccaa ccaacacggt aagcagaacc tgtcaaacct
     1621 tcatagttcc attcgaaagc ttcaaagttt ttaccagttg tgatgattgg tgctttgatt
     1681 ttacgtgaca attcagtaat cacttcacca gctttaacac caccaaatcc agcatagata
     1741 actgggcgtt cagcattgtt caagatttca acagctttgt cgatttcaac ttcgttcaaa
     1801 gcaggagcga tgaatgagcg ttcgtatgaa cctgaaccgt agtatgagtt ttcgtcgatt
     1861 tcttggaaac cgaagtttac tggaatttca acaacagctg gacctttttt agaaattgca
     1921 gcacggcagg cttcgtcaat tacttttggc aattgctcag cgtaagctac acgtttgttg
     1981 taaacagcga taccgttgta cattgggttt tggttaagct cttggaaagc atccatgttc
     2041 aattcgttaa ctggacgtga tccaaggatc gctaggaatg gagtgttatc catagctgca
     2101 tcgtaaacac cgttaatcaa gtgagtcgca cctggaccac ctgaaccaac tgcaaccccg
     2161 attgagccgc cgaatttagc ttgcataacc gctgcaagag cacctgtctc ttcgtggcga
     2221 acttgtaaga agcggatatc tttgtcttca gccaaagcgt ccatcaatga gctgagtgtt
     2281 cctgatggga taccgtagat tgtatctacg ccccatgttt tcaatacgtt aagcattgct
     2341 gcagatgcag taattttccc ttgagtcata atgataactc tccttcaatt tttttagatt
     2401 tggagaatac gattacatag aattggaaac gttctccaaa tttttactat tccactgtat
     2461 catatttatg ctgacttttc taaaaatctg ctcaaaactc tctattctct attctaatac
     2521 agttttgaaa gttctgtcat ttctgtttta taacaaagaa atctagtcat tacttttagt
     2581 ctattttact aaaatttaac agaagggaac tggtcagaac agatacagaa ctaaaggcca
     2641 tggctagacc tgccaattct gggttgagag ccagtccaac acctgaaaag actcctgctg
     2701 caatcggaat tccgacaaca ttgtagataa aagcccagaa aagattgagt agaattcgat
     2761 gaaaggtttt cttactcata tcaaaggcac gaaccactcc taaaagatta ttggttgtca
     2821 acaccaaatc tgctgactcg atggcgatat ctgttccagc tcccatagca atccccacat
     2881 ctgctacact aagggcagga gcgtcattga taccgtcccc aacaaaggct actttccctg
     2941 actgttgcag tttatggatt tcatgggctt tttcttctgg caagacgcct gcaatgacct
     3001 cttcaattcc gatttgatct gcaatagcac gcgccacacc agcattgtct cctgtcagca
     3061 tgactgttcg gagaccacgt ttttttagct gactgatggc tagcttagca ttttccttag
     3121 gaatatcttg caaagcaagc aagcctttga tttcattgtc aacagctaag aacacaactg
     3181 tcttagcttc tttttctagt tcttctagtt tatcttgata agtattagaa atatccatac
     3241 catccagcat tttagcattt ccaagtaaaa cttgttttcc attgattcgc cctgaaacac
     3301 ctttcccgtg caaggactga aaattttcaa cagtttgaaa ctcaagtcca gcttcactcg
     3361 ctcgcttaac gatagcctca gccagtgggt gttgagaagc atcttccaag gaggctgcca
     3421 atccaaacac ttctacttcg tcgccgatga tatctgttac cacaggtttc ccttccgtca
     3481 aagtcccggt cttatcaaag acaagagttt gaactttctg gatttcctgt aagacagttc
     3541 catttttgag gagaaccccc atcttggcac tacgtcctgt ccccaccata agggctgtcg
     3601 gtgttgcaag tcccaaggca caaggacagg cgataatcaa aaccgccact ccgtagagaa
     3661 gagaggacac aaagctagct ccaagcacga ccacactatc cctgagcaag acgaaccaaa
     3721 cccaaaaggt catgattcct aaaatgacaa ctactgggac aaaaatccct gaaatcttat
     3781 ccgtcaagtc ctgaatcggc gcacgacttg tctgagcttt cttcacaaaa tccacaatct
     3841 gagccaaaac agtctctgag ccaacttttt ctgctctaaa gacaagcgtt ccactatgat
     3901 tgatggttga gccaatgaca gtatctccaa ctgtcttgtc cacaggcaga ctctcacctg
     3961 tcaccatgga ttcgtcaata ctagagacac cttctactac gacaccatca acagcaatct
     4021 tttcaccggg acgcactcga atcaggtcgc ctaccttgac ttgttccaaa ggaacttgga
     4081 cataactatc atcactcaag acttctgcgg ttttagcttg caagtccagt aatttctcca
     4141 cagcttggga cgtatttttt ctcatttttt cctcaaaaac tgctcccaaa agaacgaaaa
     4201 agaggataaa tccagcactt tcgaagtaaa cagggagacc agcaaagaga gcaactaggc
     4261 tatagaaata agccactaga gttcccagcg caaccaaggt atccatgttg gcattgtgct
     4321 ttttaaaact ggcccaagca ctctggatat atggcttacc tgcaactaac ataataggcg
     4381 ttgttgctag aaaggttccc caatgcatga cttgatgact aatgctacct gtcaacatcc
     4441 caatcatgag aatcacaaga ggcacagtaa agatactagt aatccaaaaa cgttgcagga
     4501 gagatagaga ttttcgagtc ttctcaacga ctatatagct tcccttttgc atcttcatgc
     4561 cacaagaaaa ttcatgtcgc cctaattctt gaggtgtaaa acgaatgact ttctcctcat
     4621 ctacgccgat tggttccaag ataccttctt cttcaaacag aatttcctta taacagtttg
     4681 aaggagtagc acgatgaaag gtaatctcag ctggaattcc cttttgaagc tggatatggg
     4741 ctggatgata gcctttttca gctcggatac ggattttttg aatgccattt tctaagcttg
     4801 ctttcacaat ttctgtcata gtctccacct actctacaat catcttgccg tgcatcatgt
     4861 tcataccaca agcaaagcca aactctccaa cctgctcagg cgtgatttcc actacatact
     4921 cttcccccat tggcaggttc gcatgtacac caaaatctgg aaaaacaatt tgatccagac
     4981 atggtgaagg atccttgcgg tcaaagacaa tgcgtgctgg cactgatttc ttgaggacaa
     5041 tcaactcagg agtatagcct cccatgactt ccactcgaat ctcttggtat ccgttttttt
     5101 gctgggcttt ttgtccagat ttttcaggct ttttgaaaaa ccaaaacaag ataaacgcga
     5161 taagggcaat acaaataatg gttacaatac tatttaacat gacgtctcct ttacatacaa
     5221 ttacatctta cttctgttac agcacttgat ttcttctctg aaatcacagc ttccaagtct
     5281 tccaagtcag tctgagtaaa ttcacattct acaatcaagt cagccaacaa attcctaatc
     5341 ctacgggaac aaaccttgtc tttgatatct tggacaagta aatcccgact ttggtctaga
     5401 gttaaaaggg ctgaataaac aaaggacttg ccttcttttt tccgagtcag acactctttc
     5461 tcaaccaaac gagccaaaag agtttgaatg gtcgacttgg accagtcgaa ccgctctgcc
     5521 aaaaccctaa tcaaatctgt actggtctgc tccccctgca tccaaataat cttcatgacc
     5581 tgccattctg catctgaaat ctgcattacc atacctccaa aatctacatt tgtcaattac
     5641 actcatcagt atactcttaa aatctacatt tgtcaattat agaaataata ttttcttcga
     5701 aaaatagaat tttaatcatt tgaaaaacga tttgcagtca aatattacta tataaacaat
     5761 aaaaatatgc tatactaaag aaaaaagaaa acaaccacta ggggtgcgta aagctgagat
     5821 taacgactgt tagatccctc tgactcaatc taggtaatgc tagctgatgg aagtggaaat
     5881 gataatgggg actagcagtc ttctattgcc tttctaaaac agactagctt gttcttaaga
     5941 atacaaactt cagttggttg ggaggtttta gatgacttat ttacccgttg ctttgaccat
     6001 tgcagggact gaccctagtg gtggtgctgg cattatggca gatttaaagt cattccaagc
     6061 gagagatgtc tatggaatgg ctgttgtaac cagtcttgtc gctcaaaata ccagaggtgt
     6121 tcagctaatc gagcacgttt ctcctcaaat gttgaaagcc caattggaga gtgtcttttc
     6181 tgatattcca cctcaggctg taaaaactgg aatgttggct actactgaaa tcatggaaat
     6241 catccaaccc tatcttaaaa aactggattg tccctatgtc cttgatcctg ttatggttgc
     6301 tacaagtgga gatgccttga ttgactcaag tgctagagac tatctcaaaa caaacttact
     6361 acctctagca actattatta cgccaaatct tcctgaagca gaagagattg ttggtttttc
     6421 aatccatgac cccgaaggca tgcagcgtgc tggtcgcctg attttaaaag aatttggtcc
     6481 tcagtctgtg gttatcaaag gcggacatct caaaggtggt gctaaagatt tcctctttac
     6541 caagaatgaa caatttgtct gggaaagccc acgaattcaa acctgtcaca cccatggtac
     6601 tggatgtacc tttgctgcag tgattactgc tgaactagcc aagggcaaga gtctttacca
     6661 ggcagttgat aaggccaagg cctttatcac aaaagctatt caagatgccc ctcaactcgg
     6721 tcatggttct ggtccagtca accatacaac ttttaaagat taagaaaaaa actctctagt
     6781 tcccacttta agggaattag agagttttta tactcttcga aaatctcttc aaattacgtc
     6841 agcttccatc tgcaacctca aaacactgtt ttgagctgac ttcgtcagtc ttatctaaaa
     6901 cctcaaggca gtactttgag caacctgcga ctagctttct agtttactct ttgattttca
     6961 ttgagtatta attaggaaag aatgtcatgc aactttgtta aaaaggcttg cgtttttgcc
     7021 tcaatatctt ctgcttgcat caaatcacgt acaacagcta caccagctat gccagtgccc
     7081 ataagctgat caatattctc cgaagtcaag cctccaatag caactactgg aatggcaacc
     7141 gtttggcaaa ttgttttcaa ggtcgatatc agagtaatgg gcgcattttc cttggtggtg
     7201 gttgggaaaa tggctcctgt acccaagtaa tctgcacctg atttctccgc ttccagagct
     7261 cttttaaccg ttttagcggt gacaccgagg attttttcag gacccaagac tttgcgagct
     7321 accgaaactg gtaattcatc atctccgata tgcagacctg ctgcatcaac cgcaagacaa
     7381 acatccaacc gatcatcgat tatcaagggg acctgatagg catctgttat ttccttgact
     7441 tgttttgcca gttgataata ttgattggtt gtgagatttt tttctcgcaa ttggactatg
     7501 gtaacccctg aacggcaggc cgtctcaact tttgcaagaa agctttccac ggaatcttga
     7561 tagcgattgg ttaccagata tagtctaagt gcttctctat tcataaacct ctcctttgat
     7621 ggtatctagc caattttcat ctcttcttag gagcgaaagc tgattgagta cttggtaacg
     7681 aaattcttcc aatcccattc cttgaacaac tattttctca gcagcgatat tgagataaga
     7741 gactgctaag caagaagctt caaaaccagt ctttccttgg ctgagaaaaa cggctgttaa
     7801 ggctccaacc aagtctcctg tccctgttat ccagtctaat ttagtacagc cattctccag
     7861 tacagcaact tgattctccg aaacaataag gtccttgggg ccagtaacta agaaagacat
     7921 accaggatag gtctgacacc agtctttcaa gacttgaagc aaatcctccg tttcttgatc
     7981 tttagcactc gcatcgaccc caacgccgtg gtgctttaat ccaacaagac ttcgaatttc
     8041 tgacatgttt cctttaagga ccgtaggtct atagtctaaa aggtctttaa ctaagctctt
     8101 acgaatggat gaagtcgtta cgccaaccgc atctactacc atcgggagag aagattggtt
     8161 tgcatacgaa gctgccatgc ggattgcttt ttccttctca gctgacaaat gccccaaatt
     8221 gatgaagaga gcctgacttt gcttagtaaa atcaagaact tcacggggat catctgccat
     8281 gacaggtttg catcccagag ccaaaatccc atttgccagc atctcacaag aaatctcatt
     8341 ggtcatacag tgaatgaggg aactagagcc tataggaaaa ggatttgtca atgcctgcat
     8401 cagtctatcc tttcagcaaa gaaatatcct tgcacttttt taaagaattc ctgcttgatt
     8461 aaaaatctaa atgcaataaa ggaaatcgct gtaccaatca aggttgctcc gaaaaatcga
     8521 ggagtgtaga taaaccaact aagcttagca gccgatcctg taaagagcac cataacagga
     8581 taggaaacaa tagaaccaat aatacctgtt cccacaattt ctcccaaggc agaaaagtaa
     8641 aattttcgac cgtacttata aaagagacct gctagaaggg ctccaaaagt cgctcctgtg
     8701 agagctaaag gcggaatccc ttgagtcgtc atacggataa aggctgtcac tgtagccata
     8761 gccaaggcat aaacaggtcc catcatgatt cccgctagaa tattgactac actggacatc
     8821 ggtgccattc cctcaatccg aaagataggt gtaaggacta catcaagggc aatcatcata
     8881 gataaaatgg tcaatttgtg aacttgtagt tggtgctttc tcatgtttct attcttctcc
     8941 tttttctaaa gactgtaaat cgctcttcca tgtctggtgt tggtaagcca tctcccaaaa
     9001 cttggcttcc atatgaacac tgatgtggaa ggcatctagc attttttgct tatctgtctc
     9061 atcactttct cgataaagct gattgaccag tgctccctcc tctctgatct gttgctctaa
     9121 ctcatccgta atataagttt caatccattg ttgatagaga ggatttggtg atggtttaag
     9181 attaagtgat ttgcctatat catggtataa ccaaggacaa ggaagcaagc ttgcaaaagc
     9241 tatggctaag ttcggttctg caaattgcct ataaatatga gaaatgtaat gataacaggt
     9301 tggagcgatt ggatgttgct ccatttcctg gtcgttgatt tccaattcct tgaaaaattg
     9361 ttggcgaata aataactcac cctccactaa accctgagca ttttgtttca agagtctttt
     9421 catctcttgg tttgaagtct tatcagccaa aagatgatag atttctgaga aagccttcag
     9481 atagtaggca tcctgaatca ggtaatagcg gaaaatggca ggttctaaat tcccctcttg
     9541 taattgtaaa ataaagggat gatgaaagga agcctgccaa gctttcttgg ataattccat
     9601 cgcaatatct gtaaattcca taataactcc tttataaaaa tagactggtt tgaagcaata
     9661 aaaagaaaag caggtagatt aattttgttt ttttaggaat ataaaaagtc cgatagctat
     9721 tcttcaactg tgcatgttcg tcatatccgt gagcagatag agctctcagg taaagatggc
     9781 gccacctaaa gactgtcatc agaaccttac tgtaaatcaa gggcgaccaa aaatgtagtt
     9841 tttgaccacg taataggcaa gcttctttga gggacttgat ttcttgctga atgagaggaa
     9901 aagaattgaa taccacaatc aaggcatagg accaagagcg tgatagcccc ttttgagcca
     9961 agtacaagag aagctctttt agtgaaatag aggaaacaaa gacaaggccg atacaaactg
    10021 tcacaaaggc cctcgttcca agcatgactg cctgtgaagc atctccgtgt aactgaactg
    10081 cccagtagtt ggcaaaagat ggtaaaatgg caagtatgat catccaagct aacattttaa
    10141 atcgacggta atagagcata aagagaatac aaaatgcgac taccgaaaga ttcagagcaa
    10201 tcgaaggaat gaaagatgtt tccaaggata aaatcagcaa gaagagactg ataatcggtg
    10261 tctgggttgc tactttgacc atactatctc acctcccctt gggtattgct actctgagat
    10321 gtaagtggtt tggtaatggt cacttctttc acatgccgaa gaccctgact agtcatctca
    10381 atccaataat caaccacaga aatcaaaggg tctaaacgat gactaatgag cagaaaactt
    10441 cttccttgat tcctctcctc cacaatccac ttgcaaaaat aatggcaggc tctatcatcc
    10501 aaacctgcaa aaggttcatc tagcaagatc acggaagcct tactggtcaa gatggtcagg
    10561 agctgaagaa ttttttgctg accaccactt aattgatagg gactcttatc gactgcctgc
    10621 tccaaatcaa aatatcgtaa agcttgaaaa atccgctgat ttctttcaga atcaggtcca
    10681 tctaattgaa gctcctctcg cagactgact cggataaact gcttctcagc ttcctgaaca
    10741 acaccagtca gatcacgata caaactcttt ttctttttca ggaccgaacc cttccaagta
    10801 atgctcccct tatacttttg aaattgaaga atagaccgaa agagggttga tttcccgaca
    10861 ccattgtcac ccaggataca ggaaatccct tgatagaatg tgaaatcagc aattgaaaag
    10921 agggggcgat taccaagctc accagtcaca cggttcatat ggaatagttc cgggctagaa
    10981 gcaacttcct ttgaagcaac ctgtgtcatc tcataggaag ggatttgaaa cacttccctt
    11041 agttttccgt ctcttagctc caccatatgg tcgatatagg ctttatagtc agataaatca
    11101 tggtcgcaca aaataactgt cttcccatca tagaccaact cttttagaat ctccaatatc
    11161 tcgattctgc tcttgcggtc aatggaagcg aagggctcat ccaagagata gaccctagga
    11221 ttcatggcaa agaggacagc cagcgctgct ttttgctttt ccccacctga taagtgatgg
    11281 atgagacggt gcaagatgtc cttgcaacga cattgctgga caacctctgc tattttagaa
    11341 tcaatttcct gaaggtgata gccgatattt tccatggtaa aaaccaactc ctcaaacaag
    11401 ctctccatgg taaattgatg attaggattt tgcaagagaa taccaaccgt ctggacacgt
    11461 tcgacgatag aaagctgact gacctcgctc ccatctatca ggacttgacc gctataggga
    11521 agagaactaa cttgggcaat catttgaaag aggctggatt ttccagaccc actactccca
    11581 actaacaagg taaaggcttg cgcatgaaaa gtaaaatcaa acggctcaga gaagattggg
    11641 gactgaatcg ctcgtagttc cagacccatc tatgcttttc ctccagttgc aaactgatga
    11701 tagagtttga caatggcacg aaccaagatg gtacagaaga aataaacaga aataaaacgt
    11761 accacaagca aggaaaggac aaacggaagg gaaaaggcgt agtaacctaa cttaatgtat
    11821 tcatagacaa agctaacaag cgtaatccca atactattag cagttagaga gagccaactt
    11881 tcatagcgat tcttagttac gataaaacca aattcacttc ccaaaccttg aacaaagcca
    11941 gacaaaagag ctcctagacc aaattggcta ccataaagga cttcagcaag cgcagctagc
    12001 acttctccaa tcgttgcact tccgactctc ggaacaaaga tggcagcaat gggcgcagcc
    12061 atacaccaga gaccgaagag gatttcattg gcaaaggcct gcaaaccaag aggtgttaag
    12121 agtagactga gaatattata cacatatcct gaaccaacga aaaccccacc aaaaaagata
    12181 gacaagaaag caagcaagat aacatctttt aactgccatt ttttcaacat aaaaaactcc
    12241 tttttttaaa gaaaagtggg gcactcaaga agaccgacct aaatactttg tatagcagac
    12301 tgaatttaga acagtacaca agaacactaa aatatttcta gaaattaatt tgaattttct
    12361 aattgatttg ttcgcatctt atttcaatct actatatcat cttcatccag tttcgtaaaa
    12421 gaaaaaactc taattacaga tacaaattag agttcagctt acaagattag acagttcttt
    12481 tcgacatacg aaaaaaacat ttcacatttc ccttcgccag tcttaactgt atcaggttca
    12541 atgggtatca tctcagccta aagcacccca aatgtcttta ttatttaatt atgtgattat
    12601 tataacacac attttatact agttcaagaa attgaactgg aaatacagcc ttgcactcac
    12661 aaagacagca gatctttctt ttgcaaaaaa caaatgacct gtttgatgaa ttagccattc
    12721 aagctgaatc tggacatagc tttttaaaaa aggaaaatcc tacttactta gaatccaagg
    12781 atagatatct attgttcact catttcccga acagtttttt ctatattttt tgcatacgat
    12841 attgccgaaa tgattgaaac gccatccata ttggtcttta taatgtcttt aatatgtttc
    12901 gtctgtatcc caccaattgc aactaaaggc atttgtggca atagttttct catcaattca
    12961 agaccttcat aacctatagt accaccagca tcatcctttg actgggtacc aaatacaggc
    13021 ccaacaccta cataatctac atattcaact tttgattgtt gaaattcttc ttcgtttctt
    13081 atagaaagac caattatttt atctggcatc aattttctaa tttcatcaac accaatatca
    13141 tcttgaccta catgtacgcc atcggcgtca atttccattg ctaaatctat atcgtcatta
    13201 acgataaatg gaacattgta ttttttacaa agttctttaa tttggatagc tagctcaagt
    13261 ttttctaagc cttctaaagc accctcacct ttttctcgaa attgaaataa ggttatacca
    13321 ccttttaagg cttcctcaac gactgtatat agattttttc cttggcaagt agtcgttcca
    13381 caaataaaat atagttttag taattcttta tgaaacatct tacttcactc ttttgaattc
    13441 ctttacatct tcatctgtaa tctcgtataa ggcatttata aattcaactt taaatgtccc
    13501 aggaagatgt ccatttggac gtttttctgc catttctcca gcgatattgt aaaccaacat
    13561 tgctgtttct aatgatttca attcttgacc tttttctagt ccgataaagc ttgctactac
    13621 agctcctaat aagcatcctg tcccaatgac tttcggcatc atagcactac cattatgaat
    13681 cattaccact tctccattaa cagcaatggc atccacttca cctgttacta ctattggaat
    13741 attgaacttc tcatttgctg ctagagcaat ttcgtcaata ttatctacgc ccgcactatc
    13801 tactccttta gatgccacat ctattcctac taaagaggca atctcgccag catttcctct
    13861 aatcgctgct agtttataat tgttgattag atcatctgct actttttttc tatattctcc
    13921 tgctccacag gctacaggat ctaaaactgc tgggacatta tatttctctg caattttcag
    13981 agcagcttgg tataatttcc aattttcatc tgtcaatgtt cctatgttta ttaataaacc
    14041 accagcatac tttaacaaat cctctaaatc tgctggaaac tcactcatgg ctggtgaggc
    14101 gcccagtgct actaatccat ttgctgtgaa attttttact acatcattgg ttatacaaat
    14161 gaccaatggt gctttttctt ttaataattt taaacttgtc atattgaaat ccttcctttt
    14221 cactttatac gatctactga tttcgattta tttctatctt tcgttaagaa tttttttcat
    14281 ttacattgaa tgatttatac tcaatgaaaa tcaaagagca aactaggagg ctaaccgcag
    14341 gttgctcaaa acactgtttt gaggttgtgg atagaactga cgtggtttga agagattttc
    14401 gaagagtctt acctcatcaa atttgtaaat atcatgagcc ttctctagac atcgtaacca
    14461 atatcaaaaa aagctaattc taaagcgact gcttgattcc agcgttgctg aagttctgtc
    14521 aaatcttctc gatttttatc gacacgattg agttcgtcaa ccagaaattg aacccactct
    14581 gcaaagaaag gacctctgtg gagattgatc cattccgaat gaatatagac ttcaggtaaa
    14641 gccaaatctt tagaacccca gtctaaatag agaccttctg caatgaccag catgaccaaa
    14701 agatgggcat agtctgatga agccaccgcc gaatacatta gatcctgaaa ggcttttgtt
    14761 acagggtgca aagtcacttc tagatagtca ttctctgcta cttttaactc tttaaaagcc
    14821 ttttggaaat aaccatcttc atctgcttca agaaagccta gttgcttggc aaaacgaagc
    14881 ttggattcaa gtttatctgc gtgagctacg caggcaccca gcatggataa gaaggcatca
    14941 aagaagtgat aatcttgaat cagatagtcc tttaagacct tattctcaat tgtccccgca
    15001 aaaagttcct taacaaaacg atgattgatt gcagcctgcc aatccttctg actgcttttt
    15061 aataattctc caacagtcaa acctggctga aatgcatagt cttgtgtttc catatttact
    15121 tctcctctct ttacttgtta gtaattaata aaacaccaag aaatatcaag caaaatcgta
    15181 attccacttg atccttttaa agcacatcga gagcatttgc agagagctaa ctaaacaagc
    15241 ctatccagtt tatataaaca aaaaactcca attacaatca agaattagag ttgacttaca
    15301 agattagatc gttcatttcg ccatacgaaa aaacgtttca catttccctt cgccagtctt
    15361 aactgtatca ggttcaatgg gtattatctc agcctaaagc accccaaatg tctctattat
    15421 ttaattactg aaccagtata gcaaaaaatg aaagccctag caagatattt gaccgaaaaa
    15481 tatctttata tataatatat tgaaactaga atagtacacc tctacttcta aaacattgtt
    15541 agaaatcgat ttgactgtcc tgattgagtt gtcctattct tatttcattt tactatagtt
    15601 ttcgatagca atttattctt ccaatacacg aagaaaaacc tccacattca gtggaggcaa
    15661 tctgttttat caatacaatt ttaagtcacg agggtcaact gggaaggttg ggttgtatgg
    15721 attgtgacgg agcttaaagt gtttgacatc ttcaatggtc tgagctccag acaattgcat
    15781 aactgtcttc aattccgcat tcaagtgttc aaagacttga cgcacaccga cactaccacc
    15841 gagagccaag ccataaataa cagggcgacc aatagctacc aagtctgctc ctgaagccaa
    15901 ggctttaaag acgtgttgac cacgacgaac accagagtca aagacaatcg gcacacgtct
    15961 atcaactgct tctgccactt cttgaagcga gtcaaaggca gctggtccac cgtcgatttg
    16021 acgaccaccg tggttggtta cccagatacc agaagctcct gcagcaagtg aacgttcaac
    16081 gtcctcacgg cattgtggtc ccttgacata cacaggaaga ccagagtatt cagcgataaa
    16141 ttctacatcg cgtggagaca agcgttgttt agctgatttg taaacaaagt ccattgattt
    16201 accagcacct tctggcaggt attcttcaac aatcggcatg ccaactggga agacaaaacc
    16261 attacgctta tccacttcac gattcccccc tacagtagca tctgccgtca agacaatcgc
    16321 tttataacct tcagccttca cacggtccat gatgtggcgg ttgataccgt catccttact
    16381 aaagtaaaat tggaaccaat ggggtgtccc ttgaagggct tcagaaatct ctggaaggtc
    16441 aacagtagag taagagctgg ttgtataaag agaaccaaac tcatgcacac cacgcgcagt
    16501 cgccacttcc ccctgttcat ttgccaattt atgagccgca acaggcgcca taatgattgg
    16561 tgaagatagt ttttcacctg caaattcaat ctctgtactt ggattttcaa catcgcaaag
    16621 tgtatgagga acgatgagtt tgtggttaaa ggcgcggata ttctctctta aagtgaaagt
    16681 atcttccgcc ccactagcga tatagccaaa tgctgcttta ggaataactt gttgcgccat
    16741 tggctccaaa tcataggtat tgatgaagtc tacatgacct tctgcattgc ttgttttgta
    16801 tgacataaaa tgccctcctt gattaagtaa gcgtttgctt tttactacac aactataata
    16861 actctttttt caatcatttt caagagtttt gcttaaaaac catcttattt tttcatacaa
    16921 tatactagcc ctactttcct taaactctaa actttagtct atattaagtg aatcgttgag
    16981 attttttgct gccaggcatt tgaactactt ttttatttgc ggattctatc tactaatact
    17041 ataaaaaaac aagtgaataa gacaccagaa catcctctaa taggacctat ggacttatat
    17101 cagtcccata tatactgttt aagcaagata ctatcctagt tgacgctaac ttcctatccc
    17161 acttattaat tttttattta tagtgaatct gaacaaatca attggggaat ccaagtcaat
    17221 ttctagtact ttttgaacag tttcagcgta ctatcgtaaa atgaaataaa acatgcacaa
    17281 attgcttagg gaatttaaac caatttctaa caatgtctta gaaatcaaag tgtactattt
    17341 caacttcaat atactacact tcattttctc cttaaattca gtctcttttt gaatcaaaaa
    17401 atcccctggt aagagccaga ggataagaat ttatttcatt gttgggaaga gcaatacatc
    17461 acggatagtt gttgtatcag tgaggagcat gcagagacgg tcgataccga ttcccaaacc
    17521 acctgttggt ggcataccgt attcaagagc ttcaatgtag tcatagtcga tgcctgtcgc
    17581 ttcatcatca ccaagttctt tggctttagc ttgggcttca aaacggctaa gttggtcgat
    17641 tgggtcgttc aactcagtaa aggcattacc gtactcctta gtcatgataa agagctcgaa
    17701 acggtcagta aagcgttggt cttcaggatt tttcttagcg agtggagata cagctactgg
    17761 atgtccatag acaaaggttg gttggattaa agtttcttca acaaactctt caaagaaggc
    17821 attgatgatg tgaccaacct cagtgtagtg tttctcaact ggaactttct tctcagcagc
    17881 gatagtttta gcttcttcca aagtcatgtc ttgccagaaa tcgacaccag taatttctct
    17941 gatagcatcc accatatgaa cacgcttaaa tggttcgtta atcttgattt cagtaccttg
    18001 gtagttgact gggccatcac ctttgactga tttagcagcg tgttggataa tgccttcagt
    18061 caagtccatg atgtcttgga agtctgcata agcttggtaa acttcgatag aagtgaactc
    18121 agggttatga gtagcgtcca ttccttcgtt acggaagata cggccaattt catagacacg
    18181 ttccatacca cccacgataa ggcgtttcaa gtgaagctca gtcgcgatac gaagcaccat
    18241 gtcaatgttt tgggcattgt ggtgggtgat aaatggacgg gcagcagcac caccggcttc
    18301 attatgaaga acaggtgttt ccacttcaag gaatcctttt tggtcaaggt aacgacggat
    18361 ttcagagatg atttttgaac gagtgacaaa gcgttcaaag ctttcacgat tagaaatcaa
    18421 gtcaaggtaa cgtttacggt aaattgtttc aacgtctgtc aaaccatgga atttctcagg
    18481 aagaggacga agagccttag acaagtgtgt gatgtgggtt gccttgatag agagttctcc
    18541 catatccgta cgcatcactt caccttcgac accaaggaag tcaccaaggt ctgctttttt
    18601 gaagatttcg tagttttctt caccgacagc atccttacga acgtagatct gaatctggcc
    18661 ttcgcggtct tgaaggtggg caaaaccaac ttttccttta ccacgtttgg ttatcaagcg
    18721 tcctgcgata gtagctgttt cgtttttatc gtgtaattgt tctttatcga ggttggcata
    18781 tttatctttt aattcttgtg aatttgcagt acgttcaaaa cgttttccga aaggatcgat
    18841 tccttgttcg cggagcgcag ccattttttc acggcgaacg atctgctggt catttagttc
    18901 ttccatatgt tctgtagaca tgggatcctc ctagttttac tcaaaattga atacgatgag
    18961 tcattttttg cgatactccc attttatcat aaataagcaa gaaattcacg gataatcttt
    19021 aaaaactaaa aagaacctga cgctaaaatc actggaaaga agtgaaagta tctcccgccc
    19081 cactagcgat atagccaaat gctgctttag gaataacttg ttgcgccatt ggctccaaat
    19141 cataggtatt gatgaaatct acatgacctt ctgcattgct tgttttgtat gacataaaat
    19201 gtcctcctta ataagtaagc gtttactttg tgtattacaa aaatatctta actctttttc
    19261 aaaactttta aaatattttg tttggaaatt tcagaaattt tatgtctatg ataaaaatcc
    19321 ttataacggc aataaaaaat agatattatc caaagaagat tttaagtgct acaataactg
    19381 tattatttct agatgggagg ttctattttt ggattgatcc attgttgaac aatatctacc
    19441 actatatcaa aaggcattct ttctgacctt gcatattgca gtttggggaa ttttgggatc
    19501 ctttctgctc ggtttaatcg ttagtatcat ccgacattat cgaatccttg ttttggcgca
    19561 agtagcgaca gcctacattg aattgtcacg taatacgccc cttttgattc aactcttctt
    19621 tctctacttc ggtcttcccc gaatcgggat tgtcctatct tcagaagtct gtgcaacgct
    19681 tgggcttgtc tttttaggag gctcctatat ggcagaatct ttccgaagtg ggctggaagc
    19741 catcagtcaa acccagcagg agattggcct cgctattggt ctgacacctc tacaggtctt
    19801 ttactatgtg gttcttccgc aagcaacagc ggtggcactc ccctccttta gtgccaatgt
    19861 cattttcctt atcaaggaaa cctctgtttt ctcagcagtg gctttggccg acctcatgta
    19921 cgtcgccaag gatttgattg gtctctacta tgagacagac attgcgctag ctatgttggt
    19981 agttgcttat ctgatcatgc tgctacccat ctcactggtc tttagctgga tagaaaggag
    20041 gctccgccat gcaggattcg ggaatccaag tactctttca aggaaataat ctcctgagaa
    20101 tcttacaggg attgggcgtt acgattgaga tatccatcct gtctgtcctc ttatccatga
    20161 tgttcggaac agtcatggga atcatcatga cctcccattc tagaatcata cgatttttaa
    20221 cacgattgta tctggacttt acccgtatca tgccccagct ggtgctactc ttcatcgttt
    20281 actttggctt agctcgaaac tttaatatca atatctcagg tgagacttca gctattatcg
    20341 tttttaccct ctggggagca gctgaaatgg gagacttggt acgtggagct atcacttctc
    20401 tccctaaaca tcagtttgaa agtggacagg cactcggctt gactaatgtt caactttact
    20461 accacatcat catcccacaa gtcttaagaa gactgctacc gcaggctatc aatcttgtca
    20521 ctcggatgat taaaaccact tcattagttg ttttgattgg ggttgtggaa gtgaccaaag
    20581 ttggacaaca aatcatcgat agcaatcgcc tgaccatccc aactgcttca ttttggattt
    20641 atggaaccat tctagtctta tatttcgcag tttgctaccc tatttccaaa ctatccactc
    20701 acttagaaaa acattggaga aactaaatgt ctgaaactat cttagaaatc aaggaactaa
    20761 aaaaatcctt cggagacaat cccatcctcc aaggactttc tctagaaatc aaaaaaggga
    20821 agttgttgtc atcctagggc catctggttg tgggaaaagt accctccttc gttgcctcaa
    20881 cggcttagaa agtattcaag gtggagatat tcttctggat ggtcagtcta tcgttgaaaa
    20941 taaaaaagat tttcacctag ttcgccaaaa gattggcatg gtctttcaaa gttatgaact
    21001 ctttccccat ctggatgtct tacaaaacct catcctaggc cctatcaaag ctcaaggaag
    21061 ggacaagaaa gaagtaacgg aagaagcttt gcaattacta gagcgtgtcg gtttgctgga
    21121 taaacaacat agctttgccc gtcaattatc tggtggacag aagcaacgtg ttgcaattgt
    21181 ccgtgccctc ctaatgcatc cagaaatcat cctttttgac gaggtgactg cttcgctgga
    21241 tccagaaatg gtgcgtgagg tgctggaact tatcaatgat ttggcccaag aaggccgtac
    21301 catgatttta gtaacccacg aaatgcagtt tgcccaagcc attgctgacc ggattatctt
    21361 cctcgaccaa gggaaaatcg ctgaagaagg aacagctcaa gccttcttta ccaatccgca
    21421 aaccaaacga gcccaggaat ttttaaacgt ctttgacttt agccaattcg gctcatatct
    21481 ataaaggaga ttcttatgaa actattcaaa ccactcttaa ctgttttagc acttgccttt
    21541 gcccttatct ttatcactgc ttgtagctca ggtggaaacg ctggttcatc ctctggaaaa
    21601 acaactgcca aagctcgcac tatcgatgaa atcaaaaaaa aagcggtgaa ctgcgaatcg
    21661 ccgtgtttgg agataaaaaa ccgtttggct acgttgacaa tgatggttct taccaaggct
    21721 acgatattga actagggaac caactagctc aagaccttgg tgtcaaggtt aaatacattt
    21781 cagtcgatgc tgccaaccgt gcggaatact tgatttcaaa caaggtagat attactcttg
    21841 ctaactttac agtaactgac gaacgtaaga aacaagttga ttttgccctt ccatatatga
    21901 aagtttctct gggtgtcgta tcacctaaga ctggtctcat tacagacgtc aaacaacttg
    21961 aaggtaaaac cttaattgtc acaaaaggaa cgactgctga gacttatttt gaaaagaatc
    22021 atccagaaat caaactccaa aaatacgacc aatacagtga ctcttaccaa gctcttcttg
    22081 acggacgtgg agatgccttt tcaactgaca atacggaagt tctagcttgg gcgcttgaaa
    22141 ataaaggatt tgaagtagga attacttccc tcggtgatcc caataccatt gcggcagcag
    22201 ttcaaaaagg caaccaagaa ttgctagact tcatcaataa agatattgaa aaattagcaa
    22261 ggaaaacttc ttccacaagg cctatgaaaa aacacttcac ccaacctacg gtgacgctgc
    22321 taaagcagat gacctggttg ttgaaggtgg tcattaactc ttaaaaggga ctggatttta
    22381 agctccaatc cctttttaag attttaccta taacatcctg agtctatcta agatgttcaa
    22441 tctgaacaca gtgtacatac tttatcttct attgcatata ctttatcaca taagatacga
    22501 atatcctctt cactatgact agcaatcaaa attgttgtcc ctttttcact agagagcttt
    22561 ctaaacaatg ttctcatatt ttctacactt gatttatcca aggcattcat aggttcatct
    22621 agtaaaagaa tagagggatt ctccatgatt gcttgagcaa tccctagctt tttcctcata
    22681 cctagcgaat aagttttaac tttctggtct ttttgctcat atagaccaac tattttcagt
    22741 gtatcattga tttcctgatt accaactact cctcgtatgc ttgccaaata ttgtaaattc
    22801 ttaaagccac tataataatt tataaaacca ggttcttcaa tcaaagctcc caaattagct
    22861 ggaatttttc tctcaggaac aatattttcc ccattgatta acacttctcc ataagacgga
    22921 ctatataaac cagctattaa tttaaacaat acacttttcc ctgagccatt cgcaccagta
    22981 attcctataa tttccccctg tttacaacta aagttaaggt tttgaaaaac acatgtcttt
    23041 tttaatttca actcaatatt ttttaatata attatttcat tcattctata aacctcctct
    23101 tttgacgagt gaaatagaaa atgctttgaa aaagaaagac taaaaatagc aactgaagaa
    23161 acaaatctcc ccctacatcc ccattccctc gattcaaaat ataaaataga taattagttc
    23221 gatttcctac aaatagacca ccaaacacaa tcatgagtaa aaagaaacta acgcaagcaa
    23281 agttcatttg tacattcgct cctagtgcgt ataacataaa gcaaaacaag actaacacac
    23341 aagcattcaa agaatcaact ataaacaaaa ctcctatatc ccagtttggt aactgtaaca
    23401 taaattgaat cactagaact tttaccatga ttaaaatgag tccataaaga aaacaggtcc
    23461 aaatctgata cctaatccag agcttaatag aagaaaatcg aatcacttcc ataaagaaat
    23521 cggatgctct tctacgatat acaactgcta gaaaaaatgt atgaataata aaccaatgaa
    23581 gcaaccatat agataagact aaaaaagagt tttgactcag attaggccct tttaaaattt
    23641 ctataacaag tctatcattt ccttctaacc aataccataa tcctaaacaa atcaatatcc
    23701 aaaaaagaac ataaccccaa aagtacttgg tatagattat cttattcaac ctccacatgt
    23761 gactacttaa ctttcccatt tccaactttt acactctcct ccagtttcta tgaacagaaa
    23821 atacagtcaa tggaattatg atagatagta atgtaagcca ataaatcaat gcatacggat
    23881 tggaatttac catatactga aaaattggca ataatccaat tatcccattg ttcaactgat
    23941 aaatccagaa taaggattct acaaataaaa aagtaaacag aaatactaag ctcatgtttg
    24001 attttttaaa ccaagaaaaa ctgctaatag taagtaagga taaaaagaaa tagtatgcta
    24061 tataagagaa aaaaaatcct ataaagaaac tagcattgtt tgcaatactt ataccataaa
    24121 attctcttaa aaaatcaacc tcctttatct ccaaagagaa gctaaaacca ttactaaatg
    24181 caattagaaa aatcaataaa aataaagtcg ccgtccaaat ccccgtacta agagctgcta
    24241 atttgaaact aaaactggta aagtgcttaa ttgatttcag acgaatgcga cactccaacc
    24301 tattaaaata gttattcatc aaataaaaaa agaacaatat atatgtgaac ggaaagcaat
    24361 atactccagt cgtcatatct tgaagtaaaa ctaaaatcca ttctaataca tttggatgga
    24421 ttgaatattg gcgacagcgc aataaatata ctgtactaga taaaacacag gatagcagta
    24481 atataaaata aaccaatact gataaaaaat cttttttgta aaattgaaca aattgtttca
    24541 ttatacatag tcctctgaat gtagaaaaaa tgtaccataa acaaccaaac aactaacaaa
    24601 taaaataaaa gcaagatgcc cactaactaa ggaaagactg atatctttct gatatcccaa
    24661 agctaatgtt gtcacaggtt ctaagtaaga tagccctaaa atagcccaaa aaataccacc
    24721 aaccatcata taggcaactg ggatgaaaat agctcctatt tttttcttca ctagcaaagc
    24781 actagctagt ccaaaaatag agaacacagc gccccaaact ccataccaga gagtcgtcac
    24841 aagactatag agcaactgat tagaatcaaa taattctttt aaggcaccac tataatctcc
    24901 aatataaatt tcctgataag gagtcactaa aagattaatt cctaataata ataaataggg
    24961 gagaaaaaag actagaaaag aagaaataat tgcagtacta cctacaatag ccagatactt
    25021 ctttttagaa attcggcaca attgtgctgt tagaaaatga ctctcagcat cctctattat
    25081 ctgactagaa tagggcagtg tacagataag tgcagctact aggctaatcg gtgaaaatcc
    25141 tcgaatagaa gaagcggcaa aaaaaatcga taaaccttca attttataaa taccattgaa
    25201 agcaaggaaa tttcctaaac tcatgcaaag aagggtcaaa aataaagaca tataaaatcg
    25261 aggtgattga acgactcgta caagattaca aatgaaaaat tccatcctta ctcctcctta
    25321 taataaaaat agggtgtagc attctttttt catgctacac ccacaatcaa ccatctttaa
    25381 ggcttactct gacaagtaag ttaataagaa tctggactcc aagaacctga agtatgaatt
    25441 cttacatgat ttccaaattg tggcgccata gctaatctag taccagaacc aatataattg
    25501 tcaccacctc cattatagta catgacaatc ctagagccag accccaatga atataccggg
    25561 gtaatatctg acccactata ggcgctacga atagaggtat ttaacctttt accgccacca
    25621 gtgctgtcac tattattaat tccagcagag gcgttttctt tctcaagctc actagttgta
    25681 tccgaacccc aacgcggcaa cgttaaatca taatttccaa tctgtctagc taaaactgta
    25741 gggagaaaca aactagatgc aattgtaact acgcaaaaaa ggaatatatt tttcttcttt
    25801 tcatgacatc gtcctcttaa gaagtgataa acaagatagt tgattgatca atttgataca
    25861 tattacattt tcattatatc aataaaagaa agcgttgtca attattttat ttaaaatatt
    25921 gtcatatttt aaataaaatg gtaaaagaga ctcggtagcc tcgtctctct ttatcttact
    25981 gacctaacct tcttgccaat tttcttggtc ttctttaaag cgttttagaa gatcaaggcc
    26041 ctctggcgtg atgtaacctt cttcttgggc tagatggata agctctctat agtttgaaag
    26101 cgtcacaagt ttaacaccag catctgcaaa gttcttatct gcttttggca attggtagct
    26161 gaaaatcgct acaactccaa gtacatctgc tccttctcgc ttggctgctg ctacagcttc
    26221 aagaactgaa ccacccgttg aaataaggtc ttcaaccact accatttttt gaccttgagc
    26281 tacgcgacct tcgatttgat taccagctcc gtggtctttt ggtttgctac ggatgtaggc
    26341 aaaaggcaag tccatcttat cagcaataat ggctccgtgt ggaatccctg ctgttgcagt
    26401 tcctgcaatc acttctactt caggaaaggc ttctttaata gcttccacaa aaccattttc
    26461 aattagggta cgagtttctg gataggctag tgtcacacga ttatcagtgt aaatcggtga
    26521 cttgatacca gatgcccaag tgaagggttc ctctggtttg aggtaaacgg cttggatttt
    26581 caagaggtgg ctagcgatat ctttagcaag tgtcatggta ttctcctttt atttttctaa
    26641 tctagttctt taattccagt tctgtgtcca ttcatccttg atggcatgat aagctgcaac
    26701 aggatcctca gcttgggtaa tgggacgtcc cactacgata tagtcactgc cgatttgata
    26761 ggcatcagca ggtgtcatga ctcgtttttg atctccaact gcaacaccag ctggacgaat
    26821 ccctggtgtc agacagataa aatctggatt ggtagcctgc ttgatgactt gtacttcctg
    26881 agccgagcaa acaacaccat ccaagccagc ttcagctgtc ttcttggcat agtgaatcac
    26941 agactcttgc agactggttt ggatattttg aaactcctgc atctgagctt ctgacgttga
    27001 tgtgagctga gttacagcga tcaatttggc ttgactccca agaccttcac gcgccgcctt
    27061 catcatctct acaccaccag ccgcatggac attagtcata tcgacaccaa gctgagacaa
    27121 gaccttcatg gctgacttga ctgtattagg aatgtcatga agtttgagat ccaaaaagac
    27181 actatgaccc aaacctttta agtaggacac aatctcaggc cccgctgcgt aataaagctc
    27241 catccctacc ttgagataaa ggctttcttc tgctgggaaa agagctaaaa attccttgac
    27301 cgcctcaaaa ctaggaaaat caagagcaat gattggacga tgttctcgca taggtttctc
    27361 cttttacctt tactagtgag agagtctggc caacagaagc cacgaaaaaa ggaacctgcc
    27421 aacagcaagg ttccacgaaa aatgggtagt ctccaccctt tcaccgtcta accttagctg
    27481 cctctctgga ctgctttaaa ggttttctat tattctatta tttctactag cacttgtcaa
    27541 gtaaaaactt agtcacgtat gtgttgactg ctattgaaca cctcacaaga ctctttccaa
    27601 aagaatagtt aaagactttc acttaggaca gagaaaaaga gtttgcttgt taccctttaa
    27661 ttgaacaaat gggaattctg ttttattttg cggatgccta ttcatcttgg caaaggggaa
    27721 gtaatgagaa tgcgaatgga cttcttcgtg agtattttct taaaccgatt tggcaacaat
    27781 taccgaaaaa atccttaatg gtgctttgct agcttattaa tcaccgtccc caaaaatact
    27841 tggattacaa gacaggtttt gaagcactta tagaggaaat ttaaaaatgt tgcatttttt
    27901 attgcaattt atcagttatc ttatcaccca aaacttctgc aattagtatg agcactattg
    27961 ttatttttct tttttcgtac actcaaatca aaatatagca ataagactat tctgtagaat
    28021 tgctgtcaaa aagatcagtt catttttcct tcccacttgt gtttataatc attaaaataa
    28081 ctcaattaag aaattttctt tttcctaata gttatcgtaa ttaaagttaa tagaagaaca
    28141 atccaaataa gtagtgttag attaggaatt agagttttat tccatgaaaa tccctcccat
    28201 tttgtgaaaa acttagtaaa cagtcccata aaactataat ctgttatctt cgcaactgtc
    28261 ttatccaaac cagatagcat cggtaagaaa gcaactaaaa tcatagcagg aagactgata
    28321 acctgagcta ctacttggct cttcgcggtt aaacctatca ataaatagaa taaaatgatg
    28381 gataaagagg ttagaagaac gattgtaata taattaaaag tatgtacaat tgtaactcct
    28441 aaaataagag gagtagttcc cataatcaca aaagttagca aaaaaggaag aaacatcgtt
    28501 gataaaatgt attcggatcc tttaacacca ctcaacagaa gagtttgtaa attgtacttt
    28561 tctttttctt cagacaagat aatagttata ggacttccaa cagccaaagc caaagaaaaa
    28621 ggtaaacaca tcatcaaaag aactaatgcc tgttgatcgt tgaccttccc ctgtgtttcc
    28681 ataagatatt tataaaaata tgtgaaagca aaaggcacta aaacttgcaa taaaatactc
    28741 ttattggaaa ggataatttg acaacgcaac cataccaaag ccagaaaccg tttaagcatt
    28801 tagctttcct cctgttaatg tgataaaaat atcttctaaa gtaggctcac atgaatgaat
    28861 tgaaaaaata ttttccagat cagcctgaga tacctgttct agtgatgtaa aatcaaaagt
    28921 tatctgattc ccattataat ctgtaacctt aatcttttta tctttattat atctttgaat
    28981 gagttcagaa ggagctcctt gctcaactaa tttcccttta tttaataagg caacataatc
    29041 acataaaaga gttgcttcat tcatatcatg agtcgttaga aaaatcgttg tccctgctgt
    29101 tttcaattct aaaattaact catgaattgt tcgagaagtt gtgggatcta gacctgaggt
    29161 cggttcatcc agaaagagta cagcgggttt gttgataaga gctcgtgcta aaagcattcg
    29221 ttgcctcatt ccagtggata attttcctgc taccatcttg cgactatcat ataatcctac
    29281 tcgctttaac aaattatcaa cacgtgattt actaatatta taaaatttac tataaaaaag
    29341 aagattgtta tacagagaca ttttctcata aaatccactt gtatcgctaa ccaatccaat
    29401 tctctttaat tcaccgcttg ttaaattttg agatttttgt cccaaaataa tagattgtcc
    29461 tttatcggca aggaactgcc cagtcagaat attaatcgtt gtggtctttc cagaaccaga
    29521 tggtcctaaa aatctaacct tctttaacag tgaagcttat attatttaaa gctattttat
    29581 caccaaaact ttttgataaa tggctaactt caatcatact cattgcacct cctttatatt
    29641 ctacattttt acttaacatc tatttactta tctttatcaa ataaacgtct taaaaaaaac
    29701 aataacatta agcatataac cgttagcaag attgtttcaa tattattgat aatacgatac
    29761 attctcaagc agtataaaat aattatatat atgcaaatgc tcattatacc tattttttta
    29821 aacatactaa gcctccttgc taaataataa agttatataa taactaagac acactatacc
    29881 taacataata aatatccact ggtaactgaa taaatgttct aaaattaaca agcaaaaata
    29941 aaatctcaaa cgatgaaaaa agccatacta tattttccaa tgatgtttga atatttttat
    30001 tgtagtaagc ataatatata gcaaaaataa tacccaagaa caatactatt aattgtgttt
    30061 ctataacagt gaactgccca atagcagata aaaaagctac tcctcctgat aataagcaaa
    30121 aacaaactga acgaataaat gttttcgctc taatattgtt atctttcact ttattatttt
    30181 tttacaaatt ggacattctc cgttttccag aacatatttt tcttgactta gagcatgatt
    30241 tctcatatta taaacgtaag cgttatctat taggatttca ttataacatc ccagaaatat
    30301 tttaattatt aaattagtca ttatccctcc caaaatagca ggcatctctg gtgtagtcgg
    30361 ccactttgtc ttatcagata aagtgtactt atttatatct ttgtttatat tccgacaacc
    30421 aaagcaagaa ccgatggtag ggatatatat tatcagtaat aagatagcat ccattaaacc
    30481 ctcctgatat gtaggatatc ttatgcgata cagcaaattg attgacaatt tttataatat
    30541 caatgggcgt atcaattgct ttaacgataa aatctatact cccatattcc gcaacaattt
    30601 tttctaattc ttctactgaa gaaactttaa tagaaatagg tactacctga acatctgaat
    30661 tatacttgtg tattttttca gaaagaacat taatcttctc cttaccaaca tccgactctg
    30721 tataaagaat ttgcctattt aaatttgatg gttctattat atcgtaatca gcgacaataa
    30781 gtttcctcac tccatacata cttagactta gagatatata acatcctaag gctcctgctc
    30841 ctaatactaa tactgtttta ttctgtattt tattatattc ttcattataa aataacgaat
    30901 agtaaccttt agttctagaa tatctactat caatatctat ctcatccgaa attaaaattt
    30961 gttcatttaa tagttcaaat gttagtttac tgcctaatac ttcttctatc tctaatagtt
    31021 ccttacaact attaaataga tcgttgataa attcttgcga aatttgaggt tctatagcta
    31081 tttcccgcat actccgacca cgcaaataca ttataccttc ttttttataa gcaaaaacat
    31141 aactatttac gtagaacata tttcgtcctt cctctcatat ataatataga aagcagtata
    31201 gaaacaacca cccctcctat actacgaaca ataatattag gtgtcaaaaa ctgtaatagt
    31261 tgactcaaag ggaaataaat aataacaaat gctattaaaa gttgaactat atattgaaaa
    31321 ttcaagttat tattgttaga attcaagtat cttaaaataa taccactatc atactgaaaa
    31381 aaaggaattg aattaataaa aatcatacct aagttaatga taaaaaataa gagtccaagc
    31441 aatttatacg atgtgaacaa taaaacaagt aaacctgtaa gtgataaaat cgaatttgaa
    31501 gcaattcctg ctaacataat tttcaaaaat tgttttttat tttttaagaa ttgcacccca
    31561 cttaaatcta caaaaaaaag ccaaattaaa caaaagtagc attattccac aatttgggat
    31621 ctcaatttga taattattag caattattgc atgactaaat tcatgtatcg ttgcagacat
    31681 aataacaaca agcaaccaaa ataaaagaat gtttagcgtt attggaaagt tttggaaatt
    31741 agtcaaaata tccgccctat aaatatacat aataaaacat tgcaaaataa gagccaatgg
    31801 gaaaatacca aataggattt tttcaaaatt cttattaaat cttttatagt ttaatttttc
    31861 agataatgtt atattaaagt cccaagatat tttaaaaata tttatctttt ttcgtgattt
    31921 catagaatct ataagtccca ttttactaag aacaatataa aatcttaaat aatatttctt
    31981 ccctactaac actaataagt ccgatatggt aaattcttga cctattagtt gctctaacca
    32041 tttttttcct gtttcccaac taatacatgt ttcttactgg atggtatata tagatgatac
    32101 tgtccctcat aaggagtagg taatatctta atcttatcaa caattctcat aacaaaccta
    32161 acctttaata aataaaagag gggctaagta aaacatactc tagtccctca gtccgcaatc
    32221 aataaaataa tgactaattc tattaatctc ttgctgcaag actaatacgg aaaattcaaa
    32281 tgactgtcta aaatgaaatt gaaaaagctt gatgttagta aatgaacaaa cttcaacaac
    32341 cagcaagagc aaggattgct aaaccaaatc cgatacctgt tgcaagtcca gctgaaaaat
    32401 cactaactgg tactgaaatt tcttcaattt cctcaaataa aaagttatct tgcatgatta
    32461 tcactcccta tctttttaac aacaggctat tccaattaag agaactaccc cagttccaag
    32521 taaaactccc ccaaaaattc accatctgta ggggctacga tttcttcaat ttcttcaaat
    32581 aagaatgctt catctgacat atgtgttacc ttcttcataa aaaattattt tgtaatcgat
    32641 tacattgcaa atgtaacata aagaaaaaca gatgtcaaat attaaacgta agaacatggt
    32701 cactaaagaa ctataagaga aaaggtaaac ctagcgacgc gatgagcgct gggtcgtttg
    32761 atttcgattg ctctcttcct cttgtttttt ctgttcttct tcctgttttt tctcggcttc
    32821 cttggcctct tgtttggctt tttcctcagc ctccataatt aatttatccg ccacagtgta
    32881 actgtaaatt ccagcttcca tgtcgaccac actcggttct gacaattgtg gcttaatctt
    32941 actgtaatat ggcagtttct tactcatttc agatagagga accaagactt cgtccgaatc
    33001 attcatggtc aatcgaatta aatcggatgt caccttgctt ggggctaatt ccaccttttg
    33061 gatagccgcc ttgagttctg ggctaatttg agcaagttct gagacaaaaa ccttgatttg
    33121 ttcactatca ttaaagagaa ctgataaata agtttctggt aaactgttca gactcacaga
    33181 actagtctca agctgaccac tggaaagaat aggataatga ttttcaccag aaatatagta
    33241 ggccacaata tcatattcct tgaccttaat agtgaactta gttggaaatt gatagacaag
    33301 ttgagctgat tcaacccaat agttagactt aatctgcttt tcatattttg ccttgtctag
    33361 cagaaggtta atcgtataat ccgaatcctg aatgcctgaa gcctgtcgaa tatcatcagc
    33421 tgtagtttgc accgttccct caacacgaat atctttcatg gtcgcataag gactgagcaa
    33481 gtaggcagag acaatcaata aaagcagact tggaaataaa atcgtgaagg ctcgcaagat
    33541 atggatacca ggaatctttg ctttggctgg tttttccttt gtagcctttt tagaaagctt
    33601 tttatcctgt tcctccttct ctttagactc tggttcttct ttctcttctt tctctttgtc
    33661 agcctctgag gatgctactt tttcttcaga ctcttcctta gctgattctg aatcttcctg
    33721 gtctgtttca ctctcctggt cctgtttgtc ctctgacttc tcagattctt ctcccattcg
    33781 agcttgtctt tccttttcct tctcctcagc tagagccgcc tcttcttcag ccttcttttt
    33841 tagatattct tggtttcgtt tctgccattc tgataactct ttcaattctt cgaggatttc
    33901 tttgtcctca tttttcttat cttttgacat ttactttcct tatgataaat cttttttcaa
    33961 caattgataa aaatctgcta gagatttcaa ttccttagaa gccttcatct tagcttggta
    34021 atcttccttg tgacttagta agtgagaaag cttctcttcc aaactatcca aggtcaaatc
    34081 gctttcttga aggtcttctg catagccttt ttttacaaag taagctgcat tttcaagctg
    34141 gtcaccacga ctagcttcac gaccaagcgg cacaatgaca tgcaattttg ctatcgccaa
    34201 gagctcaaaa atcgtattgg caccacctcg tgtcacaaca atatcagcca attccatcaa
    34261 gggttgatag agatcggtca catagtcaac acgaaaaaga ttttggctca actcattcag
    34321 gctagaatct ccagttagat tgataatatt gtagcgctct gttagttctt tcttatggtc
    34381 tgtcaccaat tggttaaaga cacgagcacc tgcagaaccg ccaacaaaca atacagtcgg
    34441 caatttatga ttaaagtggc tttgaatatc caccaattca tctggttctg gatttttttg
    34501 atctgaaacc ttggccactg ctcccacatg ctcaacctta gacaaactcg aagcttgctc
    34561 aaaggttgaa tacatcttag tcgcaaattt ataggcgatt ttattggcca agcccataga
    34621 caggtcagat tcgtgaataa agacaggcac tcctgacaca cgcgcagcga taacaggcgg
    34681 tactgagaca aagcccccct ttgaaaaaag ggtctgtgga cgaagtcgca acatgataaa
    34741 gagcgattgg acgattcccc agcaaacttt gaagacgtcc agcatatttt gccaagagaa
    34801 atagcgacgc aattttccag tcgcaataga atggaaggtg acatccaaac ctgacttaag
    34861 gatttcttgg tgttcgatac cacgcttgtc cccgatatag tggacttccc aaccatcttc
    34921 gatgaacttg ggcattaaca aaagattgag ggtaacgtgt ccaaccgtcc ccccacctgt
    34981 aaagacaatt tttttcatat tattctttta actccgctac tgtgtcgata aagaggtcgc
    35041 cacgtacttc aaagttagca tacatatccc agctggcatt ggcaggacta agaagaacca
    35101 catctccttg agtcgcaagc tcataggcct tgcgggtcgc atctgcaata tctgtcgcct
    35161 ccacataagc gacaccagcc ttgtctgctg cccgtttgac acgttctgca gattgaccca
    35221 ggatgaccat cttcttgagt ccagtaatgt ctggcaccaa ttcgtcaaac tcattgccac
    35281 ggtccaaacc acctgcaatc aagacgacct tgctgttatc aaatcctgac aaggcttttt
    35341 gagtagccaa gatattagtt gatttactgt cgttatagaa tttaacaccc ttgatgtcat
    35401 ccacaaactg gagacggtgt ttgacaccac cgaaggctga aagagtttcc ttgatggttt
    35461 gattgtccac accacgaagc ttggctacag caatagtcgc aagggcattt tccacattgt
    35521 ggctacctgg aacaccgatt tcattcgctg ccatgactac ttcaccacgg aagtagagtt
    35581 gaccatcttc cagataagct ccatcaacct tttcaagtgt tgaaaatggt acaacagtgg
    35641 cttctgtctt ggaagtcaag tcttttgcca agtcttgatt aaagttcaag acaaggaaat
    35701 cagctgctgt catcttgttc tggatattcc acttggctgc tacatattcc gaaaatgacc
    35761 catggtagtc gatatgagtt ggcatgaggt tggtaataac cgcaatctct ggatggaatt
    35821 cttgaacacc catgagttgg aaagaagaaa gttccataac aagcgtgtcc ttatctgatg
    35881 ctatttgagc aacctgacta gctggatagc cgatattccc tgataaaaga ccatgttgcc
    35941 cagcagcagt caaaacttcc ccaatcatag tcgttgtggt tgtcttaccg ttcgatcctg
    36001 tgataccaat aatcggtgct tctgaaatca aataagccaa ttccacctca gtcaagactg
    36061 gaattccctt ggccaaagcc ttttcaatca tgggattgtt gtaggggata cctggatttt
    36121 tcaccataag ggcaaactct tcatccaaga gttccaaagg atggccacct gtaatgacct
    36181 tgatcccttc ttccagcaaa ctttgggcag ctggattgtc ctcgaaaggt ttcccatcat
    36241 ttactgtcac aatggcacct agcttgtcca acaaacgagc tgcagattca ccagacttgg
    36301 ccaaacctaa aacaaggact ttcttatttt taaattgatc tattactttc atgtctcgaa
    36361 ctccatttct actcctacta ttttaccatt tttatggaaa taaaaaagcc acaaagtgtg
    36421 tttgtgactc tttcttctaa ctgaatctta ccatatcatc tatgtgataa atcggtaact
    36481 cgaatgacct gatccacttg ttcccaaata agaggattgt gggtcgcaat aataatggtc
    36541 cgattcggat tttttaaaga ttctaggatg gaaagtaatt cctcagagtt tttggggtct
    36601 aaggaagtgg ttggttcatc tgcgaggatc aaaggtggat cctttaaaat tatcttcgct
    36661 aatgcaacac gttgtgcttc tcctcctgat aactcaaata taggttgctt caaatccaaa
    36721 taagagaggt ttacacagtt tagagcttgt ttcatcaaag agattttctc tttttccttc
    36781 aactttttac caactaaacc cagattgaga ttctctttga cggtttggct ttcaattaag
    36841 ccaaaatctt gaaataagta tcccaagtaa tctctaaaga aaacagaagg cttgatgtcc
    36901 ttaagagaag taccatcata gatgatttgt cctttgtcat atggctctaa tcgtccaatc
    36961 atattcaaga atgttgtctt accacagcca cttgtaccga ttaaggcata aattttccca
    37021 ccttcaaaat gaagatttgt atctgaaaat agctgacggc ttccaaattt tttagatata
    37081 ttctttagtt caatcatcct attttccttt cataattgtc atagaaacac gagattcttt
    37141 ctgcgcttga cggtaaagcg tcagaactgc actagctaga aagactaata aagtgagcaa
    37201 gccaatcacc aagtctcgac tgcttaaaat aaagagacta gcaccaaata caaaactggc
    37261 aaattgacta accatatact gagcatgtgt ttcaaaaaat cgtaaacctg aaattcgttt
    37321 aatcaaaata tctcggcgga attgctcgaa atatagaaga ttgacagaat aaaatagtaa
    37381 taaggaacta gctatcccca caatagctcc taagattaaa gttgctgttt cagtttgaac
    37441 ttcattataa cgagttagat aaacacttct tccttcttta agataggata cttgctcata
    37501 aattccagct ttcttcaaga gttctagccc actctcatat cctttgataa agagttgttt
    37561 tccagcattg atagaccaac tagataagga tataaaacta tcacctgtag aagtcggcgt
    37621 gaataccact aaaatcggat cagtcaaata ctgagtagat acgggattct caccgttatt
    37681 ataaacaaac cgcttttctc ccattgaaag ataactaacg tgcgctttca tctcataatc
    37741 caaaggagca cttgcctcct caccagattt tccataataa ctcaatcttt cttcaaaaac
    37801 tttcttaagt tctgcttctc gagagcgcaa atgttctggg agcaagagga taaactcacc
    37861 tttttggaga tgggctaact tctgtttggt ctcagcatct accacgacct tttccttgtc
    37921 caaataactg ggactaacat agagcgtatt agcatctgga ctataggtat ccagtgtctc
    37981 tccctgttca ttttttcctt gtggattggc aaaatggagc agattatcct ttacataaag
    38041 agcttgttct tcttcgattg cttccttggc aaaggcatac cacttgctct gattttctgt
    38101 atcttttcct ctatcaccta agccaaaaga aatttgataa tagtctgctc tatcctgcca
    38161 tgcttgtttt gaaatttcaa gttctttcaa tcgttggtaa gacatcaaac ctgtcttaac
    38221 agcgtagcct actgtaaaga cagcaaccaa ctgacacaat agggttaaaa tcatcaggcg
    38281 tttaaggggt aatctcccct taataacggg aactaatgct ttgtaactca aactcattag
    38341 ataaagaagc attagtaaaa ttgaaatcgc caataaaaac aacagataga aactaatccc
    38401 aaaaccatag gtggctaaca agataggata aaacaaacct tgactaaaaa gaacgactcc
    38461 cccacctagg aaggaaagga gggctgatag aaggagccat ttgatatcag tagataaaga
    38521 atgccccatg atggataaga gagtctgacc agaaaagagt tttatacctg ctgctctcat
    38581 ttccttaatc cgagtgataa tcactaaagc aaagaaagat aagccaaata ttgctaaact
    38641 aattaaaata aggggattta gtaatattcg aaaagcaaga aaatagggcg gtatctttcg
    38701 gtcagcactt gctttataac ccaaatctcc taatttatcg gcaagctttt ctttcgtcaa
    38761 ggagcctgac aaaaggagat aactatttag cggatcacta cgttcacgac tttcttggct
    38821 agcttcttgg aattcttttg gtaaagttcc ctgaccataa gttgcataag taaagtgagt
    38881 cgtcccatcc ttactcggct ctacaattct tctagctatt aaactctgtt ctgagtttgc
    38941 aaaattctcc aattcctgtt caaatacctc acgcgtcggt tcctgagtat cttttttgac
    39001 acgaagtaaa gaaacggaat catagcttgc atataaatat tgtggcgcac gtaagacaat
    39061 aatccaagca aggaagaagc tgagaaaaaa agttgataat aatatgaata gtttcttcat
    39121 agtagactcc ttgtaaacaa aattccccct gtaatttctt acaaggggaa cgatttaaat
    39181 caatgaacga ttagtcataa tcacagtaaa atgctacttg ttctccccat ttagtccaaa
    39241 tccatgcata ggaggttttt ccagcagaag cttctccttt atctgaatga ctattccatc
    39301 tacttacaag agaagaataa tgatattttt taccatgata gtaatttgaa aaagcccaac
    39361 cacctcctga accttctcca tacgtccata ctcctccatc tggatattgt acagcagctg
    39421 atgcagctcc caataatgta aaacttgaaa taagagctag agcaagtaat ctatgttttt
    39481 tcgttttcat tttatttttt ctttcaaaaa aagcacacct tgagcaacaa tgcaacaaaa
    39541 taaaccctcc tctctctttt gttgaaaccg ctttcttatg tgataaaaat atcattttta
    39601 ttatttgttg tcaaggaaaa aatcgaattt tttagatatt ttactacatt gcctctatga
    39661 ataatattat atagtagttt tattttaaaa taatatgcaa ccagtactaa ccaaatataa
    39721 aatagatgcc attaacgaat tttattcaag tttttcccat tcatactatc caagcaaaag
    39781 agatgatgtt atagtacaaa acaattcaaa ctattgtaaa attcctagca aaaaagagag
    39841 ccaaaactct cttttttatc ttcttttact ttttttgact ggcatgagtg taatgtctct
    39901 aacactaaag taagctagga tcaacatggc tattgctagg aatatttctg ttggtaattg
    39961 aaaaattttc agaaaagata gaaccaataa aatcaagagt gccactaaaa tacataccat
    40021 agcgacgata ttgactgtcc ctttaatgct ttctggtgtc gcaaatacat agagtaggag
    40081 cagtaaaatt cctaggacta aatagaccat ctttctctct ttctagctct tattcagctg
    40141 attttttctt cttgttagct ttctcacgct ctgctttgtt aaggatttgt ttacgcaaac
    40201 ggatagactc aggcgttact tccatgtact catcgtcgtt caagaactca agagactctt
    40261 caagtgtcaa gatacgaggc gtcttgataa cagctgtttg gtccttagta gctgaacgaa
    40321 cgttggtcat ttgttttgcc ttcgtgatgt taactgtcaa gtcattttca cgagagtttt
    40381 caccgatgat cattccttca taaacctcag tacctgggtt gacaaagatc gtaccacgtt
    40441 cttcgataga catgattgag taagttgtag ccttaccagc atcgatagaa acaagggcac
    40501 cacggtgacg tccaccaatt tcccctggaa tcaatggcaa gtattggtcg aaggtatggt
    40561 tcatgatacc gtaaccacga gtcattgaca agaactcagt tgagtatcca atcaaaccac
    40621 gcgctggaac aaggaagacc aaacgagttt gaccattacc agttgaaatc atatccaaca
    40681 tttcaccttt acgttcagaa aggctttgga taacagaccc ttggtattct tctggagtgt
    40741 cgatttgtac acgttcaaat ggttcacatt taacaccgtc gatttctttt acgataactt
    40801 ctggacgaga tacttgaagt tcatagccct cacgacgcat tgtttcgata aggattgaca
    40861 agtgcaattc tccacgtcct gaaacagtcc atttatctgg tgaatcagtt gggtcaacac
    40921 gaagggaaac gtctgtttgc aattctgcct gcaagcgttc ttccacctta cgagaagtta
    40981 cccatttacc ttctttacca gcaaatggtg agttgttgac caagaaagtc atttgaagag
    41041 ttggctcatc gatgtgtagg attggaagag cttctactgc atctgtcgga gtgatggttt
    41101 caccgacaaa gatgtcttcc atacctgaaa cggcaatcaa gtcacccgct ttggcttctt
    41161 ggatttcacg acgttccaaa ccaaagaaac cgaagagttt tgtaacacgg aagtttttag
    41221 ttgtaccgtc aagtttagaa agggtaactt ggtccccaac cttaactgta ccacggaaga
    41281 cacgaccgat accgatacgt ccaacgaagt cattgtagtc caaaagtgac acttggaact
    41341 gcaaaggctc atctgagtta tctactggag ctgggatatg gtcgataatc gtgtcaaaga
    41401 ttggtgccat agtcgcttct tggtcagctg gatcatctga caatgaagaa gttccgttga
    41461 tcgctgaagc ataaaccact gggaaatcaa gctggtcgtc atctgcacca agctcgatga
    41521 aaagttccaa gacttcatcc actacttctg ctggacgagc tgatggctta tcgattttgt
    41581 taacaaccac gattgggaca aggtcttgtt ccaaggcttt tttcaatacg aaacgagttt
    41641 gtggcatggt tccttcatag gcatctacga ccaagacaac accgtcaacc attttcatga
    41701 tacgctcaac ttctccacca aagtccgcgt gtcctggtgt gtccataatg ttgatacgag
    41761 ttccgttgta agcaacggca gtatttttag caaggatggt aatcccacgc tctttttcga
    41821 tatcgtttga gtccatagca cgctctgcca attcagtacg tgcatcaagc gtttctgatt
    41881 gtttcaataa ttcgtcaacc agggttgttt taccgtggtc aacgtgggcg ataatcgcaa
    41941 tgttacggat atcttctctt aattttgtca tgatttcctc tataatattc aaaatttatt
    42001 ttctaactga acgattatac cataatttca aataaataac ataactcaag caagtgtaaa
    42061 tgttttcact ccgcttttct tttcacgtca agccttttca aagcaagcga cttatgataa
    42121 gataggcaca gtatgcgttt agataattta ttagcccaag aaaaagttag ccgaaaggcc
    42181 atgaagcaag cactcctcag aggggaaatt ctagtcgatg gttgcccagc ccgatcccta
    42241 gctcaaaata tcgatacagg actacaagaa ctcctttttc aggatcgaat cattcaaggc
    42301 tatgaacaca cctatcttat gcttcataag cctgctggtg tcgttacagc caacaaagac
    42361 aaggaacttc cgaccgtcat ggacctgctt ccatctgaca tccagtctga caagctctat
    42421 gccgttggcc gactggaccg agatacaacg ggactcctcc tcttgaccga taacggtccc
    42481 ttgggctttc agctcctcca tccccaatat catgtcgata agacttacca agtccaggtt
    42541 aatgaacttc taacacctga ccatatccaa gcctttcaaa agggaattgt ctttttagat
    42601 ggtactgtct gtaaacccgc aaaactagag attctatctg caagtccctc cctcagtcaa
    42661 gcctctatca ccatttcaga aggaaaattt catcaaatca agaaaatgtt cctctcggtt
    42721 ggtgttaagg tgactagcct caaaagaatc caatttgggg acttcacatt gaacccagat
    42781 ttagcagaaa gtaactaccg ccctttgaac caaaaagagt tacaaatcat taaaaactat
    42841 ttagagatga gtcgataaaa caaaaaaagc tttaaaacta aagctttttt cttttattta
    42901 ccgaaaaatt aaggcgattg ctacaatcca gttaactaca gaaatcacaa ttcctaagat
    42961 attaagaatc ttttctattt tatagtctaa ttgtgactct ttttggtatg aaatagccaa
    43021 gaccaatcct atgataccca aaatcaagcc tacaattgga aataacaaac caagaataat
    43081 cgacaagata cccacaaaaa gtggattttt cttcttttct ttcatgttct aagaactctt
    43141 taaattttat acaaattaat tatactataa aacaatagct tcatcctatc attcgactaa
    43201 tttggaaata aggttagcta gtcttcactt tccctttcca agaatccaag ccataagaaa
    43261 ggatataaat ctcagaaaaa ccttgttttt tcaagtaaag agctgcattt gtaactcgtt
    43321 gcgcacgttg gttctcgtag agaaggacag gtttatcttt acgaagggct gcaagactag
    43381 ttttcaactg acttgaagga atattgcgtg caccaaggat atgttttctg tggaattctg
    43441 ctgggtcgcg caaatcaatc aattgacccg tacgaatcaa ggcttcaaac tcctcattgt
    43501 ccacaatttt agccgcacga cgaatacgaa gatagttaaa gcccatccac gccaacattg
    43561 ctagtataag tgcccacaaa atccaagtaa ccattagttc ttttctccat ttttctcaat
    43621 ataatccaat tctaccttgt gctctctgcg aagaactgct tctgcctcta gatagtctaa
    43681 tttatccatc aaccctgcat cgtaaatccg agatagttcc aacttcatca gttcaatatc
    43741 atataagcgt tttcccatgt aaacaataat accaaatcgt ttgaggaatt gctgcacatc
    43801 atagaatgtt ttcataagac tcattctagc aaaattttgt gtttttttca agaagagact
    43861 cacacaatgc tccttatttt cctatcttct ttggcgattc taaggcaagt gtggtacaat
    43921 aaaaacatga gaattcaaca attacattat attatcaaaa tcgtcgaaac tggctctatg
    43981 aatgaggcag ccaagcaact ctttatcact cagccaagtc tttccaatgc tgtgagagat
    44041 ttggaaaatg aaatgggcat tgagatcttt atccgcaatc ccaagggaat caccttgacc
    44101 cgtgatggga tggagtttct ctcttatgcc cgtcaggttg tcgagcaaac tcagcttctg
    44161 gaggaacgct ataaaaatcc tgtcgcccac cgcgaactct ttagcgtttc gtctcaacac
    44221 tatgcctttg tggtcaatgc ctttgtctct ttgctcaaga aaagcgatat ggagaaatac
    44281 gagcttttcc ttcgtgaaac tcggacttgg gagattatcg acgacgtcaa gaacttccgc
    44341 agtgaggtcg gggtcctctt cttaaacagt tacaaccgtg atgttttaac caagatgctg
    44401 gataacaatc acctactagc ccaccatctc ttcacagcgc aaccgcatat ctttgtcagc
    44461 aagaccaacc ctctggcaaa gaaagacaag gtgaaactgt ctgatttgga gaatttccct
    44521 tacctcagct atgaccaagg gacgcacaac tccttctact tttcagaaga gattctttct
    44581 caagaacacc acaagaaatc cattgtggtc agtgaccgtg ccaccctctt taatctcttg
    44641 attggtttgg atggttatac cattgcgaca gggattttga acagcaacct aaacggagac
    44701 aatatcgttt ctatcccact ggatattgat gacccgattg agctggtcta tatccagcat
    44761 gagaaaacca gcctatctaa gatgggcgaa cgctttatcg actatctact agaagaagtt
    44821 cagtttgata gttgagaaat gataagaacc aatatgtagg ctagcaacaa cctgcacatt
    44881 ggttcttttt acttataatt aaaagtttcc cctgccaact tatcagctag cttgggaaag
    44941 agagtataaa acttatgggc taggttcaac aaaatcggga gattgagttc tcgtttattt
    45001 tttcctataa tcttgacaat ctttttagcc actgcatctg cctctaagag gaagcggtca
    45061 accgatttaa gataagttcc atctgggtca gcttggtcaa aaaatcctgt tcggattgga
    45121 cctggattga ctgttgtcac atagactcca tagggcataa gttcgagtcg cagagcattt
    45181 gaaaaaccaa tagccgcaaa cttggtcgct gagtaaagac tagacttgcc agtagctatt
    45241 agacctgcca tgctgacgat gttgatgata tgccctttgc tgctttcctt catacgagcc
    45301 gcaaggtgac gagacagatt catcagggca aaggtattga cctcaaacat ctggtgaata
    45361 tctttatcag caatctggtc aaatccctca aaaatcccgt aaccagcatt gttaatcaag
    45421 acatcaatct tgccatagcg gagataaaga tcagttacca gagcttctag ggctgaatcg
    45481 tcggtaatat caatttcaat caattctgca tgggaataat ttccgtagag ttgggctaat
    45541 ttttccttat ttctaccaag caagatgagt tggtcattgg gcaggagttt gaccatttct
    45601 tgagctagac caccgctagc tccggtaatg agaatagtag gcatacttat cctttctgtg
    45661 actgctagat ttccacttct tccaagtctt tgaccacatg gacattttca aaaattgtgg
    45721 cagcgtcctt cttgagtttg cttatatctt ttgagaggaa acgggcactg atatggttga
    45781 gtaggaggcg tttggcacct gcttctaccg ctacttgtgc agcttgcata ttagttgagt
    45841 gaccatggtt acgagcaatt ttttcatcac ccttgccata agtggactca tgaactagga
    45901 catctgcatt gacagccaga cgcacactgg catccgtttt tcgagtgtct cctaaaatag
    45961 tgataatctt acctggacgt ggcgctgaga tatagtctgc tgccttgatt tcagttccgt
    46021 cttccaaaac aagatcctgg ccgtttttga ttttaccaaa aagcgggccg aacggaacac
    46081 cagcagcctt gagtttttca gcatccagcg tcccttctag atccttttgc atgacacgat
    46141 agccaacaca gaaaatagtg tggtccagct cctctgcata cacagtgaat ttatcggttt
    46201 caagaatttt acccagagaa tcttggtcaa actcatggaa atgaatgcgg tagggcagac
    46261 gagaacctga cacacgaagg ctggttaaga caaatgactt gattccttga ggtccgtaga
    46321 tttccaaatc tgtctgctct tcattggcct gaaaggcacg gctagaaagg aaacctggca
    46381 aaccaaaaat gtggtctcca tgcagatgag taataaagat tttgctgacc ttacgtggtc
    46441 gaattgtggt ttccagaatg cgattttgcg taccttctcc acagtcaaag agccaaactt
    46501 cgttaatctc gtccaaaagt ttcagggcga gacttgaaac gttgcgggct ttagagggct
    46561 gaccagcccc cgttcctaaa aattgaatat ccattcgata ctttctaatt aatcaatata
    46621 taacatggct gtgcggtttt ccgatcggaa atagcgtttg ccagaaaaag cagcagcttc
    46681 ttgcaataaa tcctcttggc tgtagccttt gagacgtttt cgaccatcag ccaatctttc
    46741 caaatcagtc aaagctgtga gactttctag gctgacaact tcctcgtcct tgacaggctt
    46801 catgtaaatc ttaccagact cttcaaagac taattgatgg gagaaaattt gcgcaatttc
    46861 aaagagcaag tcatccgaga ttttctcctc attttcagag aaaatccgac caaagccgtc
    46921 actctcataa caaaaaccaa aggatttacc agacagatta agccgaataa aaggcttatt
    46981 ttctagggtg aaacttggct cagtattgta aagattcagt tcctgactga gttctgcaaa
    47041 ataatccgtc gcagcctgag gacttttttt ctgatagagt tctgcaaagt aggcattaac
    47101 aacacttggc ggaggtgtaa taagtgttaa ctgctcctgc tctgttttac cagctagaag
    47161 ctgatccaga tagaccttgt ccagacttgt ataacctcca tactttagag ccaaagtttt
    47221 aatatcagtc ataaaattct tctaacctcc atttattttt ctcggaaatg tagcctgtaa
    47281 tcacttcgcc gtcttcctga taatcacgtt cttccagaat tgcaacactc tctaaatcat
    47341 gaatcttgta ggactttgaa aaaggcactc gcagggtaaa tgcttcaaaa atttccttaa
    47401 tcttatctag caataatgct tgcaagtttt cacgactgtc ctcagacttg gcagaaatga
    47461 gggtatatgg cgtttgggta ggcgtgaaat cctccaccaa atccgcttta ttataaagcg
    47521 tcaagtgagg aatatcttcc atgtccaggt ctttcatgat ggagagaacc gttttttcat
    47581 gctcctcgtg gtaaggattg ctagcatcga taacatgaac cagaaggtcc acatgcttgc
    47641 tttcttccaa ggttgacttg aaactggaca ccaactctgt cggcaaatct tggataaagc
    47701 caacagtatc tgtcaaagtt acttggagat tgcctcccag atgaatactc ttggttgtcg
    47761 catccagagt cgcaaagagc tcatctgctt catactgggt cttactggtc aagatgttca
    47821 tgatagttga tttcccagca ttagtataac caatcaaacc aatcttaaaa gtgctagact
    47881 ccaaacgttt ttctctgaca gtcgcacgat ttttctcaac caccttaagc tggcgctcga
    47941 tatccgtgat ttgattgcga acgctacgac ggttcagctc cagttggctt tcaccaggac
    48001 cacgggaacc aattccccct gcctgacggc tgagcataat cccctgacca accaagcgag
    48061 gcaagaggta tttgagttgg gctaggtgga cttggagctt cccttcatgg cttcgagccc
    48121 gcatggcaaa gatatccaaa atcaactgca tacggtcaat gaccttaaca ccgagaactt
    48181 cctctagatt gacattctgc cttggggtca gacggttgtt gacgatgaca gtagtgattt
    48241 cttctgcatc caccataagc gcaatctctt ccaacttacc agagccgacg aaggtcttgg
    48301 aatcatattt ttcacgtttt tgtctgtagc tatctacaac gactgcccct gccgttttcg
    48361 ctaaactagc caattcttcc atggagaggt caaaactgtc cataccctgc aattccacac
    48421 caatcagcag gactcgctcc tcttttttct ccgtttcaat catctaaaaa ctcctctatc
    48481 tggcttaaaa tgcggtcttg tacaccagat tctccaatct gataaaaggt gacctgcatg
    48541 cgattacgga accaggtcag ctgacgcttg gcaaaacgac gggtcgcctg tttaagactc
    48601 tcacgagctt cctccaaggt ctgctctcca cggaaataag gaaagagttc cttatagcca
    48661 attcctttag cagcctgtac attaggggaa tggtcaaaca gccacttggc ctcatccaaa
    48721 agcccagcct caaacatcaa atccactcgg tggttgatac gctcataaag ttgactacgt
    48781 tcatcatcca agcagataat cagcggttca tacaaaatct cttgattttc caaatcctga
    48841 ccaaaatggg caatttctaa ggcacgcata gcacgacgac gattaaactg gggaatctca
    48901 aggcctgctt gctccaccaa atgggctaat tcctcatctg aatatggctc caaactagct
    48961 cgataagcta aaatctcctc atgaggagtc tccccaccta ggtggtaacc ttctagcaag
    49021 ctctggatat aaagtccagt cccaccggcg ataatggcta gcttgccacg gctgtgaata
    49081 tcctcaatag tcatcttagc ttctgaaaca aaatcaaaag ccgagtaaga ctcagttatc
    49141 tctctaacat cgattaaatg atgaggaaca gctgcctgct cttctggact agccttggcc
    49201 gtcccaatat caagtcctcg atagacttgc tggctatctc cactaaccac ttcgccatta
    49261 aagcactttg caacttcaat agctagagcc gtctttccaa cagcagtcgg tccaacaatc
    49321 acaattattt ttgttttcat cttttttcct tgaaaaattc ccattttttc gttactatta
    49381 ttataacaca aaaaggtcag tcagaaaaat gtgacctctt gaggaggctg gctgattgag
    49441 aatataaagg aggaagactc atggctaaag gattcgctaa aggtcttgta acaggtgtcg
    49501 caggaactgt cgctgccgtt gcaggtgcag tatacgcatt taaaaagaaa gtaatcgaac
    49561 cagaagagca aaaagcagct ttcatcgaag aaaaccgtaa aaaagcagct cgtcgccgcg
    49621 tatcacgtta agaaacaaaa gaagttggga ttattctcaa cttctttttt ctattctttt
    49681 aaatagctag gtcaaacttc aactgtggct taacagggtc ataatccacc aactcaaaat
    49741 cttctgcttt gatatcaaag aaattagtcc catcaggaac atttaaaacc aagcgtggtt
    49801 ggcagtttga cggctcccga cggagcaatt cctgagcttg ttcaaattga ttatcataga
    49861 tatggaggtt gttgatgaag tagaagaact tcccaacctt ccagccaaaa tgtttagcaa
    49921 tcatcatctg caaagccaca tactgcatag cgttgatgtg gtgggccacc agcatatcat
    49981 tggagcgctg ggtcaaggtc gcatccagat agatttcccc atcaacacgc cgaacatcaa
    50041 acatggtctg aaaggcgcac gggagcagcc catctgtttc ttcgaaagct tggtaatccc
    50101 agagagaaat aatattgcgg cggttccaag gattggtttc caactgtttg agaagcttat
    50161 tgataatgtc gtgtttctta acaacggcac catagcgctc accaatggtt cccgtgtctc
    50221 caacttccca gtcattccag tagtgaacat tgtacttgtc attaagcact tctaagctat
    50281 ttgactggtc ttggtaaatc cagagaactt ctttgatggc ggatttgatt gcaatgggac
    50341 gcaaggttat gataggaaat tctcctttag ataagtcgta ctcggaaaag gcaccagtta
    50401 catacttgga attagcaaca gtcccatcct tgtacttagg acgagcctgc tctgagaaaa
    50461 caccttcctt tagaatgcgt tcaatattct ctttaaaaat cgtatctgct tttgtcattt
    50521 actctcacct cattcattgt cctaactagt atagcataaa aaataaaaga ggaacattac
    50581 taactctagg ttagcatttt cctctctcat tatcttattg caatacaagt gatgctgctc
    50641 cgataactcc agcgtcattt cctagagttg caagagctaa tttagtagat gtgcgtactt
    50701 gtgggaaagt attttcgtca tagacttttt gaacaccttg tagaaggaat tctcccgcag
    50761 ctgacacacc accaccgatg acgattgttg atgggtttag gattgaacca atgttagcac
    50821 aagcgattcc caagtaacgt gagaagttac ggtaaacaat caaagcaagg tcgtctcctt
    50881 cttttgcgag atcaaagaca gtcttagcag ttacttcttc tccgttatca atcaagcgtt
    50941 tcaaggctgc atcgccttcg tattcatcgg catagcgacg agtcaagttg acaattcctg
    51001 ttgcagaagc gactgtctca agacagcctt tcttaccgca agtacatgag attggctggt
    51061 caaagtcaac agtgatgtga cccaactcac ctgctgcacc agcaacaccg tgaagcaatt
    51121 tgccttctgc gacgataccg ccaccaacac cagtaccgag tgtcataaag acaacatctg
    51181 gttggttatc accagcaccc atccagcgct caccaagagc tgctacgttg gcatcattat
    51241 cgatgaaaaa tggaatgccc aaagcttttt caatcttttg tttgattggt tgaagggttt
    51301 tccagttcaa gttgtaggca ccgataacag tccctttgtc acggtcaacc acacctggtg
    51361 atcccattcc aatgccttgg aagtccgctg ctgccaatcc aagcaagtcc aaacgatgtt
    51421 gaatagactc aatcatatca tcaacgatat gacttccctc atccaaaatg ttggtcttga
    51481 ttgaccattt tccttggatt tctcctgctg ttgttaagat tgcaaatttg atagaagttc
    51541 caccaaggtc aatcccaata atcttttgac tcattatatt ctcctttttc attgtgtagt
    51601 aattgaaaat gcttacagta ctagtatatc aaaatccatt cacttatgca aaggtttgcg
    51661 taaatgaatt ataaggaatt ttttcgccta ccagtatctg ttaaccatct aaatgatcaa
    51721 tggcatattg agctgcatca tttgaaaatt tatcaaaatc agaagttaat tgtctataca
    51781 tacgtttttt tgacatgtgg aataacgaat tataactttt tgcctttcct aaagcagcag
    51841 aatattcact attgctatca ttactagaag aagctgtact tgcttgacct tccttccaaa
    51901 ctccgtcagc gccaacataa taacgtccac catcaaccca ttcattgaca gccattttac
    51961 catccgattt tagatagtaa tttccttgcc atgtactata agcataagaa ccatctgatg
    52021 tcaaataata ccaagcttga taggtggcat cataaaccca ttcaccttta gccattttac
    52081 catctgattt caaatagtag tttccttgcc atgcattgcg agcatatgaa ccatctgatt
    52141 tcaagtaata ccatgcttgg taagaagaat cataaaccca ctcaccttgt gccattttac
    52201 cgtttgattt aaggtaataa gctccttgcc atgcattttt tgcataagaa ccatctgatt
    52261 tcaaataata ccaagcttga taagaagagt cataaatcca ttcactctgt gccattttgc
    52321 catcagcttt caggtaatag tttccctgcc atgcattttt cactagattt ccatttccat
    52381 caaaatagta ccaagaaccg tcctgcttaa cccaaccact tgctgtggat gaagttgtat
    52441 cagtttgagt tgtagccgct tgttttgctt taaaagcacc cttaggagca tttttcaatt
    52501 cgcaagccac accatcatga tcgcggtcta acttggcaga ataaccaggt tctccctcat
    52561 gaagatccga atatccatta gcccaagctt ccttacagct cgaaaaatgg atgttttctt
    52621 ccgccaaaac tggttgtgaa aacaaggcta aaattggcaa cgtcaccaga ctcattttta
    52681 aaaatagacg tttattcatt tctttctccc ataattatga tatcgatttc aatattattt
    52741 tatcaaactt atttcagcaa ttcaaggaaa cttctggtct tttctacaaa ctcctttggt
    52801 tttgccttat tcaagacatg agggccatca ggaatgatct gaaactgcga atctgttaac
    52861 agtctatgga gagctttcat agaactaaga ttcggtttat ctttgctgcc acaaattagt
    52921 aaggttggat aggagcaagt ccctgctata tccgttaaat caagtgtctt caattcctca
    52981 gaaactccga ccataagagt cttgtctgct ccctgttttt caaatactct tttgggaagt
    53041 agtttaaaaa tcagcaactg aagataaaat aggatattcc ctgctaattt aagcgggcat
    53101 cctgacagaa tcaaagctcg aagatttggt aaatcgtaac tggaaagttc tagtgtcagg
    53161 gcagcaccta aggacaatcc aatcaaaaca aaaggttctg tctcttgagc taggtgctga
    53221 taaactcgct ctttagcttg ttgatagtta ctaactccag aaggaaataa ctcgatagcc
    53281 tcagaaggat aatctgtcag tagattccga acttctttcc aagactctgc tgactgccct
    53341 aacccatgca aaaatatcaa tttcatctag ttctcctcaa ggcttagttc atacaagcct
    53401 ctcactgcat tacagccgta aatagcttct gcttgggtta aatctgccaa ggtcaagact
    53461 ttctcttcta cctgtcctgt ttctagcaaa tactgacggt aaattcctgg taagattcca
    53521 agtcggatag gcggtgtgta gagttttcca gcgattttca gaaccaaatt tcctatagag
    53581 gtttcaagca gttctcctga cttattgtgg taaatcttct cttgttctcc taggctcaaa
    53641 tgcggtcggt gagtggtttt aaagtaggta aaggattgat tcaaagcagc ttcctgaagg
    53701 cagacttggg cctgacaaaa gcttgtactg agaggggtta atacttgacg attgacttct
    53761 atctctccag atttgctaag gctgattcgc aagcggtaat ctcgattagc ttcacaatcc
    53821 tgacactctt cctcaatctt gtgtcccaag tcttctgcat caaaaggaaa agcaaaataa
    53881 cgactagctt ttctcagtct ttccagatgt tgatcttcaa acagcaggtt cttctggctg
    53941 atttcccctg tcgtaatcag ttggaagcga gcttgtttac gataaagaac tgctgccttt
    54001 tgatgaacct ctcggtattc agattcccat gtgctatccc aagtaattcc tccgccaact
    54061 ccataaatgg cttgaccttt gtgaagttga atggtacgaa tggccacatt aaaaatccgt
    54121 cgtccatttg gaagcaagag accaatcgtt ccacagtaga ctccacgcgg ttgaggctcc
    54181 aagtccttga taatctccat tgtcgcaatt ttcggtgcac ccgttatgga accacaagga
    54241 aagagtgagc ggaagatttc aacaaggtcc acatcctctc gcaactgact cttgatggtc
    54301 gaagtcatct gccaaacagt tgaatactgc tctacctgac acagacgctc cacgtgctcg
    54361 ctcccaactt cagaaatacg gttcatatca ttgcgcaaga ggtccacaat catcatattt
    54421 tcagagcgat ttttgggatc ctgttccaac caactggcct gttcaagatc ttcttggtca
    54481 gttaccccac gctgagtcgt ccccttcatt ggtcgtgttg tcaactcgcg atcattttgc
    54541 tcaaaaaaga gctctgggct catggaaatc actgccatct cgtcatgttc cacataggca
    54601 ttgtagcccg cctcctgctc taccaccata cgattgtaga tggcaaaagg attggcactt
    54661 aacttttgct tgagttggac ggtgtagttg acctgatagg tgtctccctg cctcaagtga
    54721 tggtgtatct gggcaatggc cttttcatag tctgctgcag acgtcacttc ctgccaattt
    54781 gagggcaaat caatatcctc ataagtcaga ggaatagggg aagtttctac gctatcatga
    54841 acagtaaagt aaagcaggta ctctcccagt aggggatcct tgtgaactgc taatttttcc
    54901 tcaaaagcag gtgcagcctc gtagctgaca taccccacca cataataacc ttgctcttgg
    54961 tagctttcca cttgtgccag caaatctgcc acttcttcta catttctcgt tttcaactct
    55021 ttaataggct gggcaaaggt atatctctcc cctaaagtcc taaaatcaat cactgttttt
    55081 ctatgcatac cttaagtata gcataaaaag gcagatacag acaaagctga tctacctttt
    55141 tacgatttta ttcattctat gcttgtggtg cagtaaatag atcaccagag aaaccgtctg
    55201 tgtttttaag gagagcttta aagactttcg atttcaagac aactgtattt cggtatttgt
    55261 ctttaatact actatcgttt agttccgtta aatctttagt aacagcctca tcaaacatag
    55321 tcttcaactg ttcataggtt gaaatttgag tgccattaat ttcaattggt gcaaaacctt
    55381 ttttagattt tgtaagaact tccccatacc attgcttctt ccaatcttca aggtttgaaa
    55441 attggttatt agaaactttc ttaataatga aatcatcacc taatagaccg ttattttctt
    55501 tattggctgc accttgatgt ttgcttgaaa catagtcaat gaacccatgt tcatatccat
    55561 agtaacccca catacggaag gcgttatgtt tgaaggatag agaaccaggg gcaccttgac
    55621 tcgtattacc gccataaata ccagccatca tattaacatt gacatatgcc gtaccaaagt
    55681 cctctgggtg gaagacctta ttacctggat ttccgtgaat ggtcatgaaa ttattattta
    55741 ccaagtcatc gatggtttca aggttagttg ttttctcagc gtctatcaaa tcacgcactt
    55801 tgtcccattg gtgaggacca cttagtttat ttctgtccat tggtcgacgt gtttcgcgat
    55861 caattttctt gaaccactta ctattgtcac ctttaacctt aggaagaaca gcctcagctt
    55921 ctgcataatc gagcatcatc atagcttcat tgtagttctt catgtaatgg tcgatttggt
    55981 cacgtgtttg aagtttggta ggatctggat tataccattg gtttccatca tttgaacgtt
    56041 caaatgccat atttaatcca agtgcgccat attcaccttg atgtccatgt tctgctggag
    56101 tttgtaacat accttgagca aatgcttcaa gatctgtacc ctgtcgatgt ctatgacctc
    56161 ccagatatgc cattcggtca tttacgtggg tagtctcatg tgtaaaggca gatgtgccat
    56221 attcacttat taaatccgac ataacaaaat atacagcatc cataggctgt ggatcagcaa
    56281 agatgtaagc atacgctcca tacccatact tataatcatg atacttatca atacgtccaa
    56341 agaattcacg aataggtgca taatccggtt tatcttttcg atgaccaaat ctatcaggcc
    56401 aaccaccggg tgcaccatga ttatcccata caggagttgg aactacattt tgactcttaa
    56461 gaagtttatc gcggacatta tcagtggcaa ggcgtgacca aaaatctaag taattaactt
    56521 gctctttagc acgtttattg atttcactct tgaacgattc acgtttttct tctgtatctt
    56581 taccatactt ttcaaacgaa ctaaatgaga tagtattata tgttgaaatc ataaataaat
    56641 gtgcatcttt caagttcaga agcgataaaa tcatgcgtcc atgttgtccg ttattaagtc
    56701 cttcatatag acgatatttt ttattagcaa atgcagggtt agttgacggt tgttcaacta
    56761 cataagcatt attttcaata gctttcttaa accaaacatt tatgtctgtt tcatttgtga
    56821 atagtttcat attgtaggtc aagaagtcat gtaagtttcc agaaagagta tttttcgcca
    56881 ccacttcacg gaaggactcc tgtgtacgac ttcccttaat atagtcctct tttgaaccaa
    56941 tttctattaa tcgatctaaa acacttacat tcttaccata aaaatctggt ttgaatagca
    57001 ttaattcttt aatattgaag ttatcaaatt tcacaccgta ataacgattt aggtatgtta
    57061 atcctaacag gacagcagct ttgttatctt caattttctt aatcaaggca cgttttgcag
    57121 cttcatctgt attcagttga tgatcttcat tttcaactaa ttttgtcaca aatgtattaa
    57181 gatgatcttt tacttcttta aagctttctt ctaagtaaag attcttaatt gcattaactc
    57241 tgttatcccc agttcttcct aatcgttgat aaattggatc agattgtaat tcaattggac
    57301 ttaaaatacc ttcaatagca ctaattaaat ctgcacgatc tttaacaacc atatttggtg
    57361 tatagacgac ttcaccaagc tctgcgacac tgtattcttt tacttgctta acttgagagt
    57421 ctttaggaga aatcgtaaag atatcttttg tcttatctgc gtaatgaacc ataatatggt
    57481 cagcatctgt aaggtctgta ataaagtcat tacctttcat tgcagtcaca gacaatactt
    57541 ctttattcat taatggacta tcagttgtaa gcttgttccc ttgattcaca atccattctt
    57601 tgttgtagaa cggttggagt ttttcaacat tacgataaac aagctcacga tttgcattat
    57661 aatcttgaat tgttttgtat tcttcatcac gtaatatacg gcgattcaac ttatcaacca
    57721 aatgctcagt tacttcaaat gtatcagcag tgatacccat atccttaatt ttcttatcag
    57781 cttctacctg tgaaatttct ttaattttag acgattgttt atgggtttta tctccagaac
    57841 tcacaccgat aactgttgca ttattaggtt taatccaatt gcttacaaat tcatcatcca
    57901 tatcattaga accaaagaaa atatcaccac gcttaacatt cactaagctg atattgttat
    57961 caatggttcc ccaaggccag tctttaccaa ttaaaccgcc tacatcacct ctagcaacat
    58021 caatagtacc tttggctaca gaatttctta cattagacca agcaccaaca tctcttggat
    58081 taaaatcatg agctaaatgt cctactatag cacctgcacg agtatttaaa cctttaagtt
    58141 caacatctgc ataagcttta tttaaattgc ctcgataaat ctcagatgca atcccagcca
    58201 agttccaccc tttatcacca acagattgaa cttttccgat aaaagcaaca ttagtcataa
    58261 tacctgcatc atctaattta tccaaaatac ccgcaacatt gttattacca acgatagtac
    58321 ccgttacttt aatattttca acagttgcat ttttcactgt ttgagctaat gaagctaatt
    58381 gatttgccca aggcatatta atattaacgt taattaaatt aatatttttg atagaacccc
    58441 cttcaactct atcaaataat ggacgagcag tgttatgaat ggagaaatgc ttaccatcaa
    58501 cacttgttaa gtgacctgta aatttacctg ttacatatga tttacttggt gtagatacat
    58561 ttgttgcatt taaatccgat cctaatttaa actcaccgtt aggatttttc tgcatagcat
    58621 ctactaaatc tttgaagtta tagtaaacgt tattttgcga tgctttgggt ttctctatgt
    58681 aataagtgta tgtttgactc aaactattat ttgaatcacg ttggattaaa tcaggagctg
    58741 aagcagtaac tttaaataag gatgtctcct cctcagttac ttcctctata ttagtaatgg
    58801 caagccttgt tactttatta tgattagtgg taatttttag ataatatgac gttagatttt
    58861 ctggaacagt ttctagaagg ctactatctg tttcatttcc attctcatcc actttcatca
    58921 atcttgtttc tttaatatct ttaatctcaa cttttttaag gtctaactga acttctttat
    58981 cttctaatat ttcagtttct tcaccgttac ctcgatcata gaccatggtt gtggatagtg
    59041 tataaccttg ataatatttc aagttgtcta aagtagcttg taagttattt tcttctaccg
    59101 ttacttcttt aacaactgtt ccagtattat cttttagagc tactttaatc gatttaattt
    59161 ctactccagc tggtttagtc aatgtataac tagctgtcgc actcttcttc aagacatctt
    59221 tatctgtttt agcccattcc aaggttggtt tctctaactc ttttgtgcca ttcttaataa
    59281 ttttgtcttg aggtttttca atcactgttt ctgtgatagt aggagcgtca cttgtttttt
    59341 ctccgcgaat tgttttatag gtttcagtaa tttgtttctt atcgtttaca ccttcttgag
    59401 ttacttgtct tgttccgcgt tttaaagtgt catcttcttg ttcttttgtt tcaaaaggaa
    59461 tgatttcttc tcttgtctca accgttgtcc cttcaattgg ttttgtacca cggattacct
    59521 gttgatttac aggctccttg gtgacttccg tactggttga aagaacttgg tcagttttaa
    59581 cccctcctat tgttttatag gttgtttttg taacttgact tcctttttca ccatttcgta
    59641 ggacagtttc ttcgtccgtg tacttggttg ggtcctcagt cgtttcagtt ttatagtcaa
    59701 gttctgtagt tgattcttca acgagtgttc cttcagtcac cttatactca ggtaattgtt
    59761 ctggaacaag agcctcaccc acgtaatctt cttcttgcgt ccccttagct gttttagtct
    59821 cctcttttgg ttctacagtt tcttcagcct tggtatctgg agttgactct tcttgtttcg
    59881 gtgtctcttc cgctccccta tcttctttag gattgtcttc ttgtgatgat tcttttggtg
    59941 tacttggttg ctcagcttgt gtaggttgca cgggttgttc tgggactttt gcttgttcta
    60001 ctggtggttc aacttttgat tcctcagctg gtttgtctga tggttgactt tctggcttaa
    60061 ctgctacttt ttcctctgat tttgactcaa cttctccacc tacttcttca actggagctg
    60121 gttctgctga atcttctttc ccctcttcta ctttaggaag ggtgtcgtca gtaggtttta
    60181 cctccgattt tggttcttcc tttggacttt cttctgttct aggtgcttct tcttttggag
    60241 tttcctctgt ctctactact tggttttctg tcctagcttg ctcctgattt gttgttgatt
    60301 gaggagtctc aacttcgact acagtcacct ctccaggttt tgctgaggtt tcttctaaaa
    60361 cagtgtccaa gccaagcgtt ttgaggatgt cacctgatag ataaccaaca tagcgatagc
    60421 catccatttc aacaacaccc tctcgactag ccagcgctaa ggtcgcaact gggtctacag
    60481 cccctgcact aggaagaact accaatccca tagctccaac tagaaagacg ctagcaattt
    60541 tctttctctt gtagattaaa agcaagctcc caacagtcag caaaccaaaa gctgtcaaaa
    60601 cagatgcttc tgtccctgtt tgaggcaact gatctttttg atacaccaaa ccatatacaa
    60661 cttcattcct gtcaggcttt cctgtctgaa ttaaatcttt agcttcttgt gaaataatct
    60721 ctttatttac atagtgatag gtagctgcgt ccactacaga aggagccatc aaaaggcttc
    60781 caagaaatac agagcctaca actcccttaa tcttacgaat tgaaaaacgg tcttttttaa
    60841 acactttcat ctcctttatt cattctcaag actttctaat agcatcttgc ggatagtgcg
    60901 cacgcgcacc tccgattaat tttggacgac tagccagtgc cgttacatgg gcatgaccaa
    60961 tctctctcaa aatagggcga atcggaacct gaacatgctt gacatgcatg ccaattgcag
    61021 tgtctccgat atccaatcca gcatgagcct tgataaattc aacctcaact ggatcctgca
    61081 taaacttaaa ggctgccaac tgccccgaac ctcctgcatg aagagtagga tggacactga
    61141 caatttccag atcaaactgc tctgccacct gacgttcaac aacgagagcc cgattgacat
    61201 gctcacaacc ttgaactgct aaatggatac ctctactacc tagaataccc aagatagtct
    61261 ccactatcag ctcaccaatc tcttgactgg attctttccc aatctgacca cctagcacct
    61321 cactagaaga tagacctaaa acaaaaagga ccccctgctt caaattggtc ttttctaaaa
    61381 catcttccac tacctgacgt gtttctcttt gaatctgtgt ctcgttcatc tctgttacct
    61441 ctgttgtcac tcttctatca taccgttttt tcttgttttt agcaagatag acaacctata
    61501 aagtctgccc aattacgcat aaaactccca gaattgactg ggagttagct agtttctatt
    61561 ctatttatat atatttcaac tttcgttcct ttttggggtc tagaatcaat cttcatctgg
    61621 taattggctc caaaatgaag tttgagccgt tgatcgacat tttgaagacc aactccccca
    61681 cgtttgagtt gactttgact actatcacca gcatcttgga agccaacgcc atcatcctca
    61741 atacggatga ccaatcccga atcctgtttc tggacagaaa gtttaatatg gccctgacct
    61801 tccttttcct taatgccatg gtaaagagca ttttctacaa ggggttgtag gaccagcttg
    61861 ggtaagacta aattatcaaa ggcaacattt tcattaattt cgtattccag cttatctcca
    61921 tagcgttgtt tctggataaa gagatactgg cggacatgat tgatttcgtc agagagacaa
    61981 atcaagtcct tgccttgatt gagcgccaag cggaaatagg ttgccaagga cttggtcacc
    62041 tgcaccactc gctgactatc atgaaattca gccatccaga tgatggtgtc caaagtgtta
    62101 tagaggaaat gtggattaat ctggctcgaa agggcttgaa gttggtactg acgggtcgtt
    62161 tcttcctggc tacgaatagc taccatcaac tgatcaatct gatccaacat agcattaaat
    62221 tggcgagtta cttctctcag ttcataggca ccaacttcct tggcacgaag attttgagca
    62281 ccagaagcaa tttccaacat ggtttctctc aaatccttca aaggagcaat ccagcgttta
    62341 agactgaacc acactaagca gagacaaaca agaagagatg tgacactggc cccaagcaag
    62401 gtccacaaga gctgactccg aacctggtct aacttttcca atgatgacac gccaagtacc
    62461 gtccaatcag ttcctgcaat cttctcttga ctgacgtagg atttgtgacc aggagtataa
    62521 ccctgacctg tctcgatgta gggtttcata gcctccattt tgctagacga actataaact
    62581 gtgtgttgag gatggtagac aaattcatgg ttttcattga taatgaaggc aaagccctgc
    62641 tgccccaact ggagttgatt gagataggct tccagagttt cataagaaat atccaaacga
    62701 agcacaccaa gattggctcc ctttgcatca acaagttctt gagtgacaga aatgacccac
    62761 tgactatctg atttacgagc tggagtcaaa acaggcatag ctccctgatg aatagccttt
    62821 tggtaccaat cctcagccat catatcagag gaagttttca tctgcacact gtcatctgta
    62881 gaaatgacct gaccagattt ggtcaccagc acaacagttt tcaagtcctt atctgacttc
    62941 aagatggtca aaaacaaatc tcggattccc tcgaccttgt cttgactggg attctcagca
    63001 taggccagaa catccgtctg ctgggtcaaa ccagtcgagg tggtttctag ttttttgata
    63061 taagactgaa taaagtggct agtctggctg atggtcgttt ggctgttgcc ctcaatggtg
    63121 gcctcaatgg ctgaagaact tgattgatag tagaaagttc caaccagagc taggagaatg
    63181 agaaagacca gaaagatgga aataaccatt ctaactaaaa gagaagaacg cttcatcggt
    63241 cttctccctt cttaaactga cgaggtgtca cacctgcaat ctgcttaaaa cgttgggtaa
    63301 aatagttcat atcttcaaaa ccaaccttct ctgcgatctc ataaatcttc agatctgtag
    63361 ttaaaagcaa gagcttggct tgtttaacac gttctctcac cagataatcc tgaaaaggca
    63421 agcccaactc tttcttaatc aaggaactca gataggtcgg actaaaacct aagtcactgg
    63481 ctaaagactt taaactaaat tggctatcag ccagatgaga ctggattttc tgggccatgt
    63541 ttccttcaaa cctattagtc aataaatctt gtaactgctc ttctttctct tccttgtcta
    63601 gtttttgttt gattttcccc aacatttcct caatatcctg acgagaaaag ggtttgagca
    63661 ggtagtcgtc cacacctagt ttgacagcag acaaggcata atcaaaatca tcgtaacctg
    63721 ttaaaaagac caaatgaacc tgaggatagg tttctcgtac cagactggcc aactggatgc
    63781 catttagatg aggcatgttg atatcggtta aaatgatatc tggcacctgc ttttggatca
    63841 attcccaagc ctgccttcca ttttcagcct gaccgatgat ttccatatcg taggctgcta
    63901 cattgaccag tttagtcaaa ccttgtctta ccagatattc atcttctacg attaagattg
    63961 tgtaggtcat gctctgctcc tttaccactt actagtatca gtatagcaaa attctcctct
    64021 aactgcttag gaaagacctc ttatactcaa taaaaatcaa aaagtaaact aggaagatag
    64081 ccacaggttt ctcaaagtat agctttgagg ttgtaaataa aactgacgaa gtcagctcaa
    64141 agtatagctt tgaggttgta
//
LOCUS       DAVBGY010000004        61918 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVBGY010000004 DAVBGY010000000
VERSION     DAVBGY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 61918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61918
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.004"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            434..886
                     /locus_tag="VQ620_000235"
     CDS             434..886
                     /locus_tag="VQ620_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2762928.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            905..2242
                     /locus_tag="VQ620_000236"
     CDS             905..2242
                     /locus_tag="VQ620_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET2762929.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2321..3205
                     /locus_tag="VQ620_000237"
     CDS             2321..3205
                     /locus_tag="VQ620_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2762930.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTIFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3205..4038
                     /locus_tag="VQ620_000238"
     CDS             3205..4038
                     /locus_tag="VQ620_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2762931.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNRIASGLSEGSV
                     K"
     gene            4056..6149
                     /gene="nanB"
                     /locus_tag="VQ620_000239"
     CDS             4056..6149
                     /gene="nanB"
                     /locus_tag="VQ620_000239"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HET2762932.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6161..7264
                     /locus_tag="VQ620_000240"
     CDS             6161..7264
                     /locus_tag="VQ620_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2762933.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7438..8136
                     /locus_tag="VQ620_000241"
     CDS             7438..8136
                     /locus_tag="VQ620_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2762934.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDSPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8155..9672
                     /locus_tag="VQ620_000242"
     CDS             8155..9672
                     /locus_tag="VQ620_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2762935.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9797..11125
                     /locus_tag="VQ620_000243"
     CDS             9797..11125
                     /locus_tag="VQ620_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2762936.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11377..12243
                     /locus_tag="VQ620_000244"
     CDS             11377..12243
                     /locus_tag="VQ620_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2762937.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12259..13098
                     /locus_tag="VQ620_000245"
     CDS             12259..13098
                     /locus_tag="VQ620_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2762938.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13371..13823
                     /locus_tag="VQ620_000246"
     CDS             13371..13823
                     /locus_tag="VQ620_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2762939.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEATDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYASMEEKVRKYLFKILID"
     gene            13941..14273
                     /locus_tag="VQ620_000247"
     CDS             13941..14273
                     /locus_tag="VQ620_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2762940.1"
                     /translation="MYAARASFLDGQGITVDEMSWIINGIVTGLIGRYIKLGTYAAKY
                     GISMARSILSRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAVAWDAHDKI
                     PNNGRINF"
     gene            14560..15204
                     /locus_tag="VQ620_000248"
     CDS             14560..15204
                     /locus_tag="VQ620_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET2762941.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15373..16290
                     /locus_tag="VQ620_000249"
     CDS             15373..16290
                     /locus_tag="VQ620_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET2762942.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKESFL"
     gene            16308..17192
                     /locus_tag="VQ620_000250"
     CDS             16308..17192
                     /locus_tag="VQ620_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2762943.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17208..18059)
                     /locus_tag="VQ620_000251"
     CDS             complement(17208..18059)
                     /locus_tag="VQ620_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2762944.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18259..20316
                     /gene="pbp2b"
                     /locus_tag="VQ620_000252"
     CDS             18259..20316
                     /gene="pbp2b"
                     /locus_tag="VQ620_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HET2762945.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSELTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20327..20923
                     /gene="recR"
                     /locus_tag="VQ620_000253"
     CDS             20327..20923
                     /gene="recR"
                     /locus_tag="VQ620_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HET2762946.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21099..22142
                     /locus_tag="VQ620_000254"
     CDS             21099..22142
                     /locus_tag="VQ620_000254"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET2762947.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQ
                     SGDFIKTQEFSHDPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALELAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22226..23599
                     /locus_tag="VQ620_000255"
     CDS             22226..23599
                     /locus_tag="VQ620_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET2762948.1"
                     /translation="MKLTIHEIAQVVGVKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQDLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGNQSVQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     RLENEVK"
     gene            23694..24398
                     /locus_tag="VQ620_000256"
     CDS             23694..24398
                     /locus_tag="VQ620_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HET2762949.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIVKEA"
     gene            24617..25990
                     /gene="ftsA"
                     /locus_tag="VQ620_000257"
     CDS             24617..25990
                     /gene="ftsA"
                     /locus_tag="VQ620_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET2762950.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26007..27266
                     /gene="ftsZ"
                     /locus_tag="VQ620_000258"
     CDS             26007..27266
                     /gene="ftsZ"
                     /locus_tag="VQ620_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET2762951.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27271..27942
                     /locus_tag="VQ620_000259"
     CDS             27271..27942
                     /locus_tag="VQ620_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET2762952.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            27952..28491
                     /locus_tag="VQ620_000260"
     CDS             27952..28491
                     /locus_tag="VQ620_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HET2762953.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTSVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            28491..28754
                     /locus_tag="VQ620_000261"
     CDS             28491..28754
                     /locus_tag="VQ620_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET2762954.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            28751..29536
                     /locus_tag="VQ620_000262"
     CDS             28751..29536
                     /locus_tag="VQ620_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET2762955.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            29545..30333
                     /gene="divIVA"
                     /locus_tag="VQ620_000263"
     CDS             29545..30333
                     /gene="divIVA"
                     /locus_tag="VQ620_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HET2762956.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            30681..33473
                     /locus_tag="VQ620_000264"
     CDS             30681..33473
                     /locus_tag="VQ620_000264"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET2762957.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRSELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
     gene            33876..34261
                     /locus_tag="VQ620_000265"
                     /pseudo
     CDS             33876..34261
                     /locus_tag="VQ620_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34796..35488
                     /locus_tag="VQ620_000266"
     CDS             34796..35488
                     /locus_tag="VQ620_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HET2762958.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            35541..37952
                     /locus_tag="VQ620_000267"
                     /pseudo
     CDS             35541..37952
                     /locus_tag="VQ620_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
     gene            38202..38903
                     /locus_tag="VQ620_000268"
     CDS             38202..38903
                     /locus_tag="VQ620_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2762959.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANCDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            38905..41613
                     /locus_tag="VQ620_000269"
     CDS             38905..41613
                     /locus_tag="VQ620_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET2762960.1"
                     /translation="MIKRKTYWKDLVQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAS
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIITKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSLLASVLPAYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFIHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTTKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(41709..42200)
                     /gene="tpx"
                     /locus_tag="VQ620_000270"
     CDS             complement(41709..42200)
                     /gene="tpx"
                     /locus_tag="VQ620_000270"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET2762961.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEDLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(42323..43252)
                     /gene="psaA"
                     /locus_tag="VQ620_000271"
     CDS             complement(42323..43252)
                     /gene="psaA"
                     /locus_tag="VQ620_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HET2762962.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(43284..44132)
                     /locus_tag="VQ620_000272"
     CDS             complement(43284..44132)
                     /locus_tag="VQ620_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET2762963.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(44129..44851)
                     /locus_tag="VQ620_000273"
     CDS             complement(44129..44851)
                     /locus_tag="VQ620_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET2762964.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            45151..47043
                     /gene="pepO"
                     /locus_tag="VQ620_000274"
     CDS             45151..47043
                     /gene="pepO"
                     /locus_tag="VQ620_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HET2762965.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPILSLLKEFQELETFADF
                     IAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(47166..47795)
                     /locus_tag="VQ620_000275"
     CDS             complement(47166..47795)
                     /locus_tag="VQ620_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET2762966.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            48789..51011
                     /locus_tag="VQ620_000276"
     CDS             48789..51011
                     /locus_tag="VQ620_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET2762967.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            51041..51484
                     /locus_tag="VQ620_000277"
     CDS             51041..51484
                     /locus_tag="VQ620_000277"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HET2762968.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKRGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(51523..51884)
                     /locus_tag="VQ620_000278"
                     /pseudo
     CDS             complement(51523..51884)
                     /locus_tag="VQ620_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014307.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(51956..52369)
                     /locus_tag="VQ620_000279"
     CDS             complement(51956..52369)
                     /locus_tag="VQ620_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2762969.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFECLLLSGLIFFLA
                     QGLLLFLLIVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMS
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            complement(52471..53208)
                     /locus_tag="VQ620_000280"
     CDS             complement(52471..53208)
                     /locus_tag="VQ620_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HET2762970.1"
                     /translation="MKKSLKIFATSKWFDLFGVALVVGIAIASGYLNSRLDKFVDWGS
                     WTALVPFGLISVTNVGISMLSTRFTGKLSKWGNYFGIVNTLLFGAIDYILGNKAAIIT
                     YPVTFLIYTFAIKKWEASQEGRPNQMSQKQVKLAAIIISIIAFLFAFVTNYIGYEGKM
                     DLLAYVTTIAFALSLIANALNALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFY
                     ILNAIGALFVWNDEEVRYIEGNSNKEY"
     gene            complement(53328..53978)
                     /locus_tag="VQ620_000281"
     CDS             complement(53328..53978)
                     /locus_tag="VQ620_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET2762971.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            54090..54932
                     /locus_tag="VQ620_000282"
     CDS             54090..54932
                     /locus_tag="VQ620_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET2762972.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            55008..55445
                     /locus_tag="VQ620_000283"
     CDS             55008..55445
                     /locus_tag="VQ620_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET2762973.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            55513..55815
                     /locus_tag="VQ620_000284"
     CDS             55513..55815
                     /locus_tag="VQ620_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2762974.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(55906..56979)
                     /locus_tag="VQ620_000285"
     CDS             complement(55906..56979)
                     /locus_tag="VQ620_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HET2762975.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            57135..57812
                     /locus_tag="VQ620_000286"
     CDS             57135..57812
                     /locus_tag="VQ620_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2762976.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            57805..58779
                     /locus_tag="VQ620_000287"
     CDS             57805..58779
                     /locus_tag="VQ620_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET2762977.1"
                     /translation="MLDWKQFFLAYLRSRGRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(58828..60771)
                     /gene="thrS"
                     /locus_tag="VQ620_000288"
     CDS             complement(58828..60771)
                     /gene="thrS"
                     /locus_tag="VQ620_000288"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET2762978.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(60829..61005)
                     /locus_tag="VQ620_000289"
     CDS             complement(60829..61005)
                     /locus_tag="VQ620_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HET2762979.1"
                     /translation="MTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENF
                     LSDKEKQLNVRTRK"
     gene            complement(61012..61152)
                     /locus_tag="VQ620_000290"
     CDS             complement(61012..61152)
                     /locus_tag="VQ620_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HET2762980.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            complement(61583..61783)
                     /locus_tag="VQ620_000291"
     CDS             complement(61583..61783)
                     /locus_tag="VQ620_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2762981.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKELYINNLL
                     FFTPLVILIISLLYSYRFRFSL"
BASE COUNT        18631 a        11232 c        13775 g        18280 t
ORIGIN      
        1 ttagaaaaac gaagtaagca gcttataaat tcaagtcccc aaatagattc atactagtat
       61 cttttgcaaa aaataaaggg cgacttcctt catgaatatc aatttcatct ataaggaagg
      121 tagctaattg aactaactta tttattctgt ttgtcgctag aaaaatcaga cctccttgtg
      181 aagattgagg agatacttaa tgaaaatcaa agaagaaact agcaagctag tagcagattg
      241 cccaaaacac cgctttgagg ttgtagataa gactgaccta tataatccaa ggtgaagcga
      301 ctgtggtttg aagagatttt caaagagtat aggctagaga gtagtgtttt tatgtccttc
      361 tagtagaaaa tgctagacag aagaatgggg aacttggata ggaaaaatag attgagaaag
      421 gaggttagaa gagatgatta ttacaaaaat tagccgttta ggaacttatg tgggagtaaa
      481 tccacatttt gcaacattaa tagattttct agaaaaaaca ggactagaaa atttaacaga
      541 aggttcgatt gctatcgatg gtaatcgatt gtttgggaat tgctttactt atctagcaga
      601 tggtcaagca ggggctttct ttgaaacaca ccaaaaatat ttggatattc atttagtttt
      661 ggaaaacgaa gaagccatgg ctgttacatc gccggaaaat gtaagcgtta cccaagaata
      721 tgatgaagag aaagatattg aattatacac agggaaagtg gaacagttgg ttcatttgag
      781 agctggtgaa tgcctcatca cttttccaga agatttacat caacccaagg ttcgtataaa
      841 tgatgaacct gtgaaaaaag ttgtctttaa agttgcgatt tcttaatgta gaaagagaag
      901 aacgatgaaa aaaatgagaa agtttttatg tctagctgga attgcgctag cggctgttgc
      961 cttggtagct tgttcaggaa aaaaagaagc tacaactagt actgaaccac caacagaatt
     1021 atctggtgag attacaatgt ggcactcctt tactcaagga ccccgtttag aaagtattca
     1081 aaaatcagca gatgctttca tgcaaaagca tccaaaaacg aaaatcaaga ttgaaacatt
     1141 ttcttggaat gacttctata ctaaatggac tacaggttta gcaaatggaa atgtgccaga
     1201 tatcagtaca gctcttccta accaagtaat ggaaatggtc aactcagatg ctttggtgcc
     1261 gctaaatgat tctatcaagc gtattggaca agataaattt aacgaaactg ccttaaatga
     1321 agcaaaaatc ggagatgatt actactctgt tcctctttat tcacatgcac aagtcatgtg
     1381 ggttagaaca gatttgttaa aagaacataa tattgaggtt cctaaaactt gggatcaact
     1441 ctatgaagct tctaaaaaat tgaaagaagc tggagtttat ggcttgtctg ttccgtttgg
     1501 aacaaatgac ttaatggcaa cacgtttctt gaacttctac gtacgtagtg gtggaggaag
     1561 cctcttaaca aaagatctta aagcagactt gacaagccaa cttgctcaag atggtattaa
     1621 atactgggtt aaattgtata aagaaatctc acctcaagat tctttgaatt ttaatgtcct
     1681 tcaacaagct accttgttct atcaaggaaa aacagcattt gactttaact ctggcttcca
     1741 tatcggagga attaatgcca acagtcctca attgattgat tcgattgatg cttatcctat
     1801 tccaaaaatc aaagagtctg ataaagacca aggaattgaa acctcaaaca ttccaatggt
     1861 tgtttggaaa aattcaaaac atccagaagt tgctaaagca ttcttagaag cactttataa
     1921 tgaagaagac tacgttaaat tccttgattc aactccagta ggtatgttgc caactattaa
     1981 ggggattagc gattctgcag cctataaaga aaatgaaact cgtaagaagt ttaaacatgc
     2041 tgaagaagta attactgaag ctgttaaaaa aggtactgct attggttatg aaaatggacc
     2101 aagtgtacaa gctggtatgt tgactaacca acacattatt gaacaaatgt tccaagatat
     2161 cattacaaat ggaacagatc ctatgaaagc agcaaaagaa gcagaaaaac aattaaacga
     2221 tttatttgag gctgttcagt agatgtaaaa gactagaaaa taggtgggct agtgagctga
     2281 aaagctctag cccaatcttg taaaagaagg gagaaggaga atggttaaag aacgtaattt
     2341 aactcgctgg atatttgttt tgccagctat gattatcgta ggattactct ttgtttatcc
     2401 gtttttctcg agtatttttt atagctttac caataagcat ttgattatgc ctaattataa
     2461 atttgttggt ttggctaact ataaagctgt gctatcagat cccaacttct ttaatgcgtt
     2521 ctttaattca attaagtgga ccattttctc attagttggt caagttttag tagggtttgt
     2581 attggcttta gctcttcaca gagtacgcca cttcaagaaa ttatatagga ccttattgat
     2641 tgttccttgg gcatttccta ccatcgttat tgccttctct tggcagtgga ttctaaacgg
     2701 ggtttatggc tacttaccta atctaatcgt aaaattaggt ttaatggaac atacacctgc
     2761 atttttgaca gatagtacat gggcattcct atgtttggtg tttatcaaca tttggtttgg
     2821 agcaccaatg attatggtta atgtgctttc agctttgcaa acagtaccag aagaacaatt
     2881 tgaggctgct aagatagatg gtgcttcaag ttggcaggtg ttcaagttta tcgtctttcc
     2941 acatattaaa gtggttgtag gacttctagt tgttttgaga actgtatgga tctttaataa
     3001 ctttgacatt atctacctca ttactggtgg tggaccagcc aatgctacaa cgacgcttcc
     3061 aatttttgct tacaacctgg gttggggaac taaattgttg ggtcgtgctt cagcagttac
     3121 agtattgctc tttatcttct tggtggcgat ttgctttatc tactttgcta tcatcagtaa
     3181 gtgggaaaag gagggtagaa aataatgaag aagaaatcca gtatttattt agatattctc
     3241 tcacatgtac ttttagttgg tgcgaccatc gttgcagttt tcccattggt atggattatc
     3301 atatcttctg tcaaagggaa aggggaatta actcagtatc caacacgatt ttggcctgaa
     3361 cagtttacat tagattattt cactcatgtt atcaacgatt tgcacttcat tgataacatt
     3421 cgaaacagtt taatcattgc cttggctaca acccttattg cgattattat ttctgctatg
     3481 gcagcctatg gtattgttcg attctttcct aaattgggag caatcatgtc gagactactc
     3541 gtcattacct acattttccc accaattttg ttagcaattc cctattcaat tgccattgct
     3601 aaagttgggt taacaaatag tttatttggc ttgatgatgg tttatctatc ttttagtgtt
     3661 ccatatgcag tttggctctt agttggattt ttccaaacag ttccaattgg aattgaagaa
     3721 gcggctagaa ttgatggtgc aaataaattt gttacgtttt ataaagttgt gctaccgatt
     3781 gtagcaccag gtattgtagc aacagctatt tatacattta tcaatgcttg gaatgaattc
     3841 ctgtatgcct tgattttgat taacaataca ggaaagatga cagtagcagt agcccttcgt
     3901 tcacttaatg gttcagaaat actagactgg ggagatatga tggcagcgtc tgttattgta
     3961 gttcttccat caattatttt cttctctatc atccaaaata ggattgcaag tggattatca
     4021 gaaggatctg tgaagtagac gaaagaagga aaaaaatgaa taaaagaggt ctttattcaa
     4081 aactaggaat ttctgttgta ggcattagtc ttttaatggg agttcccact ttgattcatg
     4141 cgaatgaatt aaactatggt caactgtcca tatctcctat ttttcaagga ggttcatatc
     4201 aactgaacaa taagagtata gatatcagct ctttgttatt agataaattg tctggagaga
     4261 gtcagacagt agtaatgaaa tttaaagcag ataaaccaaa ctctcttcaa gctttgtttg
     4321 gcctatctaa tagtaaagca ggctttaaaa ataattactt ttcaattttc atgagagatt
     4381 ctggtgagat aggtgtagaa ataagagacg cccaaaaggg aataaattat ttattttcta
     4441 gaccagcttc attatgggga aagcataaag gacaggcagt tgaaaataca ctagtatttg
     4501 tatctgattc taaagataaa acatacacaa tgtatgttaa tggaatagaa gtgttctctg
     4561 aaacagttga tacatttttg ccaatttcaa atataaatgg tatagataag gcaacactag
     4621 gagctgttaa tcgtgaaggc aaggaacatt acctcgcaaa aggaagtatt gatgaaatca
     4681 gtctatttaa caaagcaatt agtgatcagg aagtttcaac tattcccttg tcaaatccat
     4741 ttcagttaat tttccaatca ggagattcta ctcaagctaa ctattttaga ataccgacac
     4801 tatatacatt aagtagtgga agagttctat caagtattga tgcacgttat ggtgggactc
     4861 atgattctaa aagtaagatt aatattgcca cttcttatag tgatgataat gggaaaacgt
     4921 ggagtgagcc aatttttgct atgaagttta atgactatga ggagcagtta gtttactggc
     4981 cacgagataa taaattaaag aatagtcaaa ttagtggaag tgcttcattc atagattcat
     5041 ccattgttga agataaaaaa tctgggaaaa cgatattact agctgatgtt atgcctgcgg
     5101 gtattggaaa taataatgca aataaagccg actcaggttt taaagaaata aatggtcatt
     5161 attatttaaa actaaagaag aatggagata acgatttccg ttatacagtt agagaaaatg
     5221 gtgtcgttta tgatgaaaca actaataaac ctacaaatta tactataaat gataagtatg
     5281 aagttttgga gggaggaaag tctttaacag tcgaacaata ttcggttgat tttgatagtg
     5341 gctctttaag agaaaggcat aatggaaaac aggtccctat gaatgttttc tacaaagatt
     5401 cgttatttaa agtgactcct actaattata tagcaatgac aactagtcag aatagaggag
     5461 agagttggga acaatttaag ttgttgcctc cgttcttagg agaaaaacat aatggaactt
     5521 acttatgtcc cggacaaggt ttagcattaa aatcaagtaa cagattgatt tttgcaacat
     5581 atactagtgg agaactaacc tatctcattt ctgatgatag tggtcaaaca tggaagaaat
     5641 cctcagcttc aattccgttt aaaaatgcaa cagcagaagc acaaatggtt gaactgagag
     5701 atggtgtgat tagaacattc tttagaacca ctacaggtaa gatagcttat atgactagta
     5761 gagattctgg agaaacatgg tcgaaagttt cgtatattga tggaatccaa caaacttcat
     5821 atggcacaca agtatctgca attaaatact ctcaattaat tgatggaaaa gaagcagtca
     5881 ttttgagtac accaaattct agaagtggcc gtaagggagg tcaattagtt gtcggtttag
     5941 tcaataaaga agatgatagt attgattgga aataccacta tgatattgat ttgccttcgt
     6001 atggttatgc ctattctgcg attacagaat tgccaaatca tcacataggt gtactgtttg
     6061 aaaaatatga ttcgtggtcg agaaatgaat tgcatttaag caatgtagtt cagtatatag
     6121 atttggaaat taatgattta acaaaataaa ggagaaaaac atggttaaat acggtgttgt
     6181 tggagcaggg tattttggag ctgaattggc tcgctacatg caaaaaaatg atggagcaga
     6241 gattactctt ctctatgatc cagataatgc agaggcgatt gcagaagaat tgggagcaaa
     6301 agtagcaagt tccttagatg agttggtttc tagcgatgaa gtagactgtg ttatcgtcgc
     6361 aactccaaat aatcttcata aggaaccggt tattaaggct gcacagcatg gtaaaaatgt
     6421 tttctgtgaa aaaccaattg cgctttctta tcaagattgt cgcgagatgg tagatgcgtg
     6481 taaagaaaac aatgtaacct ttatggcagg acatattatg aatttcttta atggtgttca
     6541 tcatgcaaaa gaactcatta atcaaggagt tatcggagac gttctatatt gtcatacagc
     6601 tcgtaatggt tgggaagaac aacaaccgtc agtgtcatgg aaaaaaattc gtgaaaaatc
     6661 aggtggtcac ttgtatcacc acatccatga attggattgc gttcaattcc ttatgggggg
     6721 catgcctgaa actgtaacca tgacaggtgg aaatgtggcc catgaaggtg aacatttcgg
     6781 tgatgaagat gatatgattt ttgtcaatat ggaattttct aataagcgtt ttgccttgtt
     6841 agaatggggt tcagcttatc gttggggtga acattatgtc ttaatccaag gaagcaaagg
     6901 tgccatccgc ttagacttat tcaactgtaa aggaactctt aagctagatg ggcaagaaag
     6961 ctatttcttg attcacgaat cgcaagaaga agatgatgat cggactcgta tctatcatag
     7021 tacagagatg gatggagcaa ttgcttatgg taaaccaggt aaacgtactc cattatggct
     7081 atcatctgtc attgataaag aaatgcgcta tctgcatgag attatggaag gagccccagt
     7141 atcagaagaa tttgcaaaac ttttgacagg tgaagctgcc ctagaagcaa ttgctactgc
     7201 agatgcttgt acccagtcta tgtttgaaga tcgcaaagta aaattgtcag aaattgtaaa
     7261 ataaattttg gtattctcct atttataggt cgacttgctc ctctgaaagt acttttagag
     7321 gagctgtttg actttgctag tttttgaaac tgaaatctat tatactacaa actattgaaa
     7381 gcgttttaat tttaaggtat aataatctca tagaaataaa gaaaaggagg aaagaggatg
     7441 ccacagatta gcaaagaagc cttgattgag caaatcaaag atggaatcat cgtttcttgt
     7501 caggctcttc ctcatgaacc gctttataca gaagcgggag gggtgattcc cttgctggtc
     7561 aaagcggctg agcaaggtgg agcagtcggt atccgagcaa acagtgttcg cgatatcaag
     7621 gaaattaagg aagtcactaa acttccaatc attgggatta tcaaacgtga ttatccacct
     7681 caggaaccct tcatcacggc tactatgaaa gaagttgatg aattggcaga actggacatc
     7741 gaggtgattg ctctggattg taccaagcgt gaacgctacg atggtttgga aattcaagag
     7801 ttcattcgtc aggttaagga gaaatatcct aatcagcttt tgatggctga tactagtatc
     7861 ttcgaagaag ggctagcagc tgtagaagca ggaattgact ttgtcggaac aaccttatca
     7921 ggctacacat cctacagtcc aaaagtagac agtccagatt ttgaattgat taagaaactc
     7981 tgtgatgccg gtgtagatgt cattgcagaa ggaaaaattc atacaccaga acaagccaaa
     8041 caaatccttg aatatggagt gcgaggcatc gttgttggtg gcgccattac tagaccaaaa
     8101 gagattacag aacgcttcgt tgctagtctt aaataagatg tgagagggag ttttatgttt
     8161 aaagttttac aaaaagttgg aaaagctttt atgttaccta tagctatact tcctgcagca
     8221 ggtctacttt tggggattgg tggtgcactt tcaaacccaa ccacgatagc aacttatcca
     8281 atactagaca atagtatttt tcaatcgata ttccaagtaa tgagctctgc aggagaggtt
     8341 gtattcagta atttgtcact acttctctgt gtgggattat gtattggctt agcgaaacga
     8401 gataaaggaa ccgctgcgtt agcaggagta actggttact tagttatgac tgcaacgatc
     8461 aaagctttgg taaaactttt tatggcagaa ggatctgcaa ttgatactgg agttattgga
     8521 gcattagttg tcggaatagt tgccgtatat ttgcacaacc gatataacaa tattcaatta
     8581 ccttccgctt taggattctt tggaggttca cgcttcgttc ctattgttac atcgttctct
     8641 tctatcttga ttggctttgt cttctttgtt atttggccac ctttccaaca acttcttgtt
     8701 tctacaggtg gatatatttc tcaggcgggt ccaattggaa cttttctata tggattttta
     8761 atgagacttt ctggagcagt aggcttacat catataattt accctatgtt ttggtatact
     8821 gaacttggtg gtgttgaaac tgttgcagga caaacagtgg ttggagctca aaaaatattt
     8881 tttgctcaat tagccgatcc ggcccattct ggattattta cagaaggaac aaggtttttt
     8941 gcaggtcgtt tctcaacaat gatgttcggt ttaccggctg cctgtttagc gatgtaccat
     9001 agtgttccta aaaatcgtcg taaaaaatac gcgggtttgt tttttggagt tgctttaaca
     9061 tcttttatta ccggtattac agaaccaatt gaatttatgt ttctattcgt cagtccggtt
     9121 ctatatgttg ttcacgcatt ccttgatggt gttagcttct ttattgcaga cgtcttaaat
     9181 atttcaatag gaaacacatt ttcaggaggt gtaatcgatt tcactttatt tggaattttg
     9241 caggggaacg ctaagacgaa ttgggttctt cagattccaa ttggacttat ttggagtgtt
     9301 ttgtattata ttatttttag atggttcatt actcaattca acgttctaac gccaggacga
     9361 ggagaagaag tagattctaa agaaatttct gaatccgcag attcaacttc aaatactgca
     9421 gattatttaa aacaggatag cctacaaatt atcagagcct tgggtggatc aaataatata
     9481 gaagatgtag atgcttgtgt gacacgttta cgtgtagctg taaaagaagt taatcaagtt
     9541 gataaagcac ttttaaaaca aattggtgca gttgatgtct tagaagtgaa gggtggtatt
     9601 caagcaatct atggagcaaa agcaatctta tataaaaata gtattaatga aattttaggt
     9661 gtagatgatt aagtacttac tgacttaata aaaaacagag gagagtggtg gatgagtagg
     9721 atgaaatgaa atcgcataca agaaataaag aactcattat ccaagttgga tacgcttatt
     9781 acataggaga atacaaatga aatttagaaa attagcttgt acagtacttg cgggtgctgc
     9841 ggttcttggt cttgctgctt gtggcaattc tggcggaagt aaagatgctg ccaaatcagg
     9901 tggtgacggt gccaaaacag aaatcacttg gtgggcattc ccagtattta cccaagaaaa
     9961 aactggtgac ggtgttggaa cttatgaaaa atcaatcatc gaagcgtttg aaaaagcaaa
    10021 cccagatata aaagtgaaat tggaaaccat cgacttcaag tcaggtcctg aaaaaatcac
    10081 aacagccatc gaagcaggaa cagctccaga cgtactcttt gatgcaccag gacgtatcat
    10141 ccaatacggt aaaaacggta aattggctga gttgaacgac ctcttcacag atgaatttgt
    10201 taaagatgtc aacaatgaaa acatcgtaca agcaagtaaa gctggagaca aggcttatat
    10261 gtatccgatt agttctgccc cattctacat ggcaatgaac aagaaaatgt tagaagatgc
    10321 tggagtagca aaccttgtaa aagaaggttg gacaactgat gattttgaaa aagtattgaa
    10381 agcacttaaa gacaagggtt acacaccagg ttcattgttc agttctggtc aagggggaga
    10441 ccaaggaaca cgtgccttta tctctaacct ttatagcggt tctgtaacag atgaaaaagt
    10501 tagcaaatat acaactgatg atcctaaatt cgtcaaaggt cttgaaaaag caactagctg
    10561 gattaaagac aatttgatca ataatggttc acaatttgac ggtggggcag atatccaaaa
    10621 ctttgccaac ggtcaaacat cttacacaat cctttgggca ccagctcaaa atggtatcca
    10681 agctaaactt ttagaagcaa gtaaggtaga agtggtagaa gtaccattcc catcagacga
    10741 aggtaagcca gctcttgaat accttgtaaa cggatttgca gtattcaaca ataaagacga
    10801 caagaaagtc gctgcatcta agaaattcat ccagtttatc gcagatgaca aggagtgggg
    10861 acctaaagac gtagttcgta caggtgcttt cccagtccgt acttcatttg gaaaacttta
    10921 tgaagacaaa cgcatggaaa caatcagcgg ctggactcaa tactactcac catactacaa
    10981 cactattgat ggatttgctg aaatgagaac actttggttc ccaatgttgc aatctgtatc
    11041 aaatggtgac gaaaaaccag cagatgcttt gaaagccttc actgaaaaag caaacgaaac
    11101 aatcaaaaaa gctatgaaac aatagtcctt agttattcta taaaaagtag ttttttaaag
    11161 aacctaagag tgtatacccc cttttccctc tacacagata gtgtaagaaa agggggcttt
    11221 tgtttaaaat gtaagaaact gtcacgaaat taaaatgaag ttcttacata agcgaatcat
    11281 aaaaaatttc attttgattt taaaacagtt caagaaagtc aaaaaattat tctatttgaa
    11341 agagaggtgc cgactgtgaa agtcaataaa atccgtatgc gggaaacagt gatttcctac
    11401 gctttcctag caccagtatt attcttcttt gtcatctttg tgttggctcc gatggtgatg
    11461 ggcttcatta caagtttctt taactactca atgactaaat ttgagtttgt aggcttggat
    11521 aactatatcc gtatgtttaa agatcctgtc tttacaaaat ctctgattaa cacagttatt
    11581 ttggttattg gatctgtacc agttgttgtt ctattctcac tctttgtagc atctcagacc
    11641 tatcatcaaa atgtcattgc cagatccttc taccgtttcg tcttcttcct tcctgttgta
    11701 acgggtagtg ttgccgtgac agttgtttgg aaatggattt atgacccact atcagggatt
    11761 ctaaactttg tccttaagtc aagccacatc atcagccaaa acatttcttg gttgggagat
    11821 aaaaactggg cattgatggc gattatgatt attctcttga ccacttcagt tggtcagccc
    11881 atcatccttt atatcgctgc catggggaat attgacaatt cactggttga agcggcgcgt
    11941 gttgatggtg caactgagtt tcaagttttc tggaagatta aatggccaag ccttcttcca
    12001 acaactcttt atattgcaat catcacaaca attaactcat tccagtgttt cgccttgatt
    12061 cagcttttga catctggtgg tccaaactac tcaacaagta ccttgatgta ctacctttac
    12121 gaaaaagcct tccaattgac agaatacggc tatgccaaca caattggtgt cttcttggca
    12181 gtcatgattg ctatcgtaag ctttgttcaa tttaaagtac ttggaaacga cgtagaatac
    12241 taaagaaagg agacagctat gcaatctaca gaaaaaaaac cattaacagc ctttactgtt
    12301 atttcaacaa tcattttgct cttgttgact gtgctgttca tctttccatt ctactggatt
    12361 ttgacagggg cattcaaatc acaacctgat acaattgtta ttcctcctca gtggttccct
    12421 aaaatgccaa ccatggaaaa cttccaacaa ctcatggtgc agaaccctgc cttgcaatgg
    12481 atgtggaact cagtatttat ctcattggta accatgttct tagtctgtgc aacctcatct
    12541 ctagcaggtt atgtattggc taaaaaacgt ttctatggtc aacgcatcct atttgctatc
    12601 tttatcgctg ctatggcgct tccaaaacaa gttgtccttg taccattggt acgtattgtc
    12661 aacttcatgg gaatccacga tactctctgg gcagttatct tgcctttgat tggatggcca
    12721 ttcggtgtct tcctcatgaa acagttcagt gaaaatattc ctacagagtt gcttgaatca
    12781 gctaaaatcg acggttgtgg tgagattcgt accttctgga gtgtagcctt cccgattgtg
    12841 aaaccagggt ttgcagccct tgcaatcttt accttcatca atacttggaa tgactacttc
    12901 atgcaattgg taatgttgac ttcacgtaac aatttgacca tctcacttgg ggttgcgacc
    12961 atgcaggctg aaatggcaac caactatggt ttgattatgg caggagctgc ccttgctgct
    13021 gttccaatcg tcacagtctt cctagtcttc caaaaatcct tcacacaggg tattactatg
    13081 ggagcggtca aaggataata ctctgcgaaa atcgaatgca aactacgtca gcttcacctt
    13141 gccatactta agtattgcct gcggttagct tcctagtttg ttcttcaatt ttcattgagt
    13201 ataggaaaat caatctatca agatacagaa gtatatttta tagatttaga gaatatagaa
    13261 gttataagtg tctacaaaat ggagggtatg cagttacttt atgaagtttt gtcagacact
    13321 tatcaactta agaatggttt tagttaacta tcagaaacga aggaaagagt atgatttttg
    13381 acgatttgaa aaacatcacc ttttacaaag ggattcatcc caatttagac aaggctatcg
    13441 actacctcta ccaacatcgt aaagattcat tcgaattagg aaagtatgag attgatggag
    13501 ataaagtctt tctagttgtt caggaaaatg tcctcaatca agttgagaat aatcaatttg
    13561 aacaccataa gaactatgca gatttgcatt tgctgataga agggcatgaa tattcgagct
    13621 acggttcacg tatcaaagac gaggcagtag cattcgacga agcgactgac attggctttg
    13681 ttcattgtca tgaacactac cccctcttgt tgggttatca caattttgcg attttcttcc
    13741 caggtgagcc acatcagcca aatggttatg caagcatgga agaaaaggtt cggaaatatc
    13801 tctttaaaat tttgattgat taaaaatagg atgaattgtt tttttgtaaa gctttgacaa
    13861 tactctacca tgaaattgat ctttgtgagg tagggaaatg agaataaaat atttaaaaat
    13921 tggtatcttc ttgtcagtct gtgtatgctg caagagctag tttcttagat ggacagggga
    13981 ttacagttga tgagatgtcc tggataatta atggcattgt gactggattg attggtagat
    14041 acataaaatt aggtacttat gcagctaagt atggtattag tatggcgcgc tcgatcttaa
    14101 gtagggcagc tgcaactgcg gcagcaagag taggattatt gactaagatt tttggatgga
    14161 ttttacgaat agctgtgctc gcagctgatg tatatggtaa ttttgccaac aatattgctg
    14221 tagcttggga tgcacatgat aaaatcccta acaatggtcg tataaacttt taaaatgcga
    14281 gaatgaaagc actttgtatt tttttattga atatgttagc ttggacagtg cttgcaatga
    14341 tagtccgtgg agggctagat ggatttaatg agcatacttg gagtactatt ttaattgctt
    14401 cgctgtccgg gatatatata gattatatgt ccatagataa aagtagaaaa aagtccaaaa
    14461 gaaaaaagaa aaaatagatt tgttcatggt agggacttat gaaagtttta ctgacaaaaa
    14521 agaaaacagt ttacaaagaa aaatgatgga ggagcaaaca tggcacaaaa aggagtaagc
    14581 cttatcaagg cagcatttga tacagataac tttctcatgc gttttagtga gaaagtcttg
    14641 gacatcgtga cagccaatct tctttttgtc gtctcttgtt tgcccatcgt gacgattgga
    14701 gtggctaaaa tcagcctcta cgagaccatg ttcgaagtta agaagagcag acgggtgcct
    14761 gtttttaaaa tctatctaag atctttcaag caaaatctga aactaggtct tcagctgggt
    14821 ttaatggagt taggaattgt gtttcttacc ctttcagatc tctatctttt ctggggtcaa
    14881 acagctctgc ccttccaatt gctgaaagcc atttgtttag gtattctgat ttttcttact
    14941 atcgtgatgc tggctagtta ccctatcgcg gcacgttatg acctatcttg gaaagaaatt
    15001 cttcaaaaag gattgatgtt ggctagtttt aactttcctt ggttcttcct catgttagcc
    15061 attcttgtcc tcattgtgat ggttctttat ctgtccgcct tcagtctact cttaggtggc
    15121 tcagtcttcc tactttttgg gtttggacta ttggtcttta tccagactgg attgatggag
    15181 aaaattttcg caaaatacca ataggagctt tatttctgaa actactttca aaggctccaa
    15241 acgctattct ataagcgaga aactaaaatc taagtgtcta taagatattg aaagcctttt
    15301 cggtaaagtg tatactaaag aagtaaagaa aaatctgctt tagcagaaaa taaaaataat
    15361 aggagaaaat ctatgtcaga tttgaaaaaa tacgaaggtg tcattccagc cttctacgca
    15421 tgttatgatg atcaaggaga agtaagtcca gagcgtacgc gtgccttggt tcaatacttc
    15481 attgataaag gtgttcaagg tctttatgtc aatggttctt ctggtgaatg tatctaccaa
    15541 agcgttgaag atcgcaagtt gattttggaa gaagtcatgg cggtagccaa aggtaaattg
    15601 accattattg cccatgttgc ttgcaataat actaaagata gtatggaact tgctcgccat
    15661 gctgaaagct tgggagtaga tgctattgca acgattccac caatttattt ccgcttgcca
    15721 gaatactcag ttgccaaata ctggaacgat atcagttctg cagctccaaa cacagactac
    15781 gtgatttaca acattcctca attggcaggg gttgctttga ctccaagcct ttacacagaa
    15841 atgttgaaaa atcctcgtgt tatcggtgtt aagaactctt ctatgccagt tcaagatatc
    15901 caaacctttg tcagccttgg tggagaagat catatcgtct ttaacggtcc agatgagcag
    15961 ttcttaggtg gtcgtctcat gggagctaaa gctggtatcg gtggtactta tggtgctatg
    16021 ccagaactct tcttgaaact caatcagttg attgcggata aggacctaga aacagcgcgt
    16081 gaattgcagt atgctatcaa cgcaatcatt ggtaaactca cttctgctca tggaaatatg
    16141 tacggtgtca tcaaagaagt cttgaaaatc aatgaaggtt tgaatattgg atctgttcgt
    16201 tcaccattga caccagtgac tgaagaagat cgtccagttg tagaagcggc tgctgccttg
    16261 attcgtgaaa ccaaggagag cttcctctaa tctaaaagga ggtatttatg acatactacg
    16321 ttgcaattga tatcggtgga accaacatca agtatggttt ggttgatcaa gaggggcaac
    16381 ttcttgaatc gcatgaaatg ccaactgagg cgcataaggg tggacctcat atcttacaaa
    16441 agaccaaaga tatcgtagct agttatttag aaaaaggccc agtagcaggt gttgccatat
    16501 cttctgctgg gatggtggat ccggataagg gtgagatttt ctatgctggg ccgcaaatcc
    16561 ctaactacgc aggcacccag ttcaaaaagg aaatcgaaga aagctttact attccttgtg
    16621 agattgaaaa tgatgtcaac tgtgcaggtc ttgctgaggc agtatctggt tcaggcaagg
    16681 gagcaagtgt gacactttgc ttgaccattg gaacaggtat cggtggttgc ttgattatgg
    16741 ataggaaagt cttccatggt tttagcaatt cagcctgtga agtcgggtat atgcatatgc
    16801 aggatggagc ttttcaagac ttggcttcta caacagcttt agtgaaatat gtagctgaag
    16861 cccatggaga agatgttgat cagtggaatg gccgtagaat tttcaaagaa gccactgaag
    16921 gaaacaaaat ctgcatggaa ggtattgacc gtatggttga ctatctagga aaaggtctgg
    16981 caaatatttg ctacgttgcc aatccagaag tggttattct tggtggtggt atcatggggc
    17041 aagaggctat cctcaaacct aagatccgca cagccttgaa agaggctttg gtaccaagtt
    17101 tagcagaaaa aacacgatta gaatttgccc atcaccaaaa tacagcaggg atgttgggtg
    17161 catattatca ttttaagaca aaacaatcct agtttggctc agccaaacta ggattttctt
    17221 acacgttttt gtctacgata gccgttgagt tttttatttt cccagtagct attaaagatt
    17281 ttttccttgc tttcgcgatt gatttccaaa aagtaggcat aaatcaaatc gataaagaag
    17341 agcataggaa gttgagcgga tattcgttgg atataggagg gttggctgtg ggtggctaca
    17401 agaacagtct ctgtataggt ctggctatct ttattgggaa cacttgtaaa gagtacagtc
    17461 tttgccccca tctccttggc atctaataga ctatctaaaa tagaaggagt tgagcctgaa
    17521 agtgagaagc ccagtactag acaattttca tccatgatgc tggttgtcca ggcaaagccg
    17581 tcttggtctg tcaaagcttc gcagaccaca cctagtcgca taaaacgtaa tttcatttca
    17641 cgggcgacga ggccagaact ccctgttcca aagaagtaga tacgctcagc atcttcgatt
    17701 agctgggcaa ttcgctctag ttggatttcg tcaatcaagt cttgtgtttg ttccctcata
    17761 ttgctataac ttctgaggac tcgtttggtc agtggactgt gcttggagac ttggttggct
    17821 tgattttctg cctgatgttg gtattggaaa ataaattctc ggtagccagt aaagccacac
    17881 tttttagcaa agcgggtcaa agcagcttga gaaatatgta atttttgggt gacttgttga
    17941 gaagataaat catctgtaat cgtttcagct tgcaaaaaat agcgagcgat ttcttgctct
    18001 aggtctgtca tttcttcaaa atgtgaatca atgatagttg cgatatctgg tttgtccata
    18061 gggaaagctc ctttacatga gtcatactgg aagactagat cagggaatag tcacacttca
    18121 ttataacaca taatataagg atagataaat aaaaacgcat ttctgtttta aaaacgaaaa
    18181 aatcgaaaaa gcttctttct tttccataat tttctactca aattgtggta caattaagag
    18241 taagatttta agttagaaat gagactgatt tgtatgagaa aatttaacag ccattcgatt
    18301 ccgattcggc ttaatttatt gttttcaatc gtcattttac tctttatgac cattattggt
    18361 cgtttgttgt atatgcaggt tttgaacaag gatttttacg aaaaaaagct agcctcagct
    18421 agtcagacca agattacaag cagttcagcc cgtggggaaa tttatgatgc tagtggaaaa
    18481 cctttggtag aaaatacgtt aaagcaggtt gtttccttta cgcgtagcaa taaaatgacg
    18541 gctacagact taaaagaaac agctaaaaag ttactgactt atgtgagcat cagttctcca
    18601 aatttgacag aacgccagct ggcggattac tatttggctg atcctgaaat ctataaaaaa
    18661 acagtggaag ctctcccaag cgagaaacgc ttggattcag atggcaatcg tctatccgaa
    18721 tcagaactgt ataacaatgc ggtcgatagt gtccaaacaa gtcaactaaa ctatacagag
    18781 gatgaaaaga aagaaatcta tctttttagt cagttaaatg ctgttggaaa ctttgcgaca
    18841 ggaaccattg ctacagatcc tctaaatgat tctcaggtgg ctgttattgc ctctatttca
    18901 aaggagatgc ctggcattag tatttctact tcttgggata gaaaggtttt ggaaacttcc
    18961 ctttcttcta tagttgggag tgtatccagt gaaaaagctg gtctcccagc ggaagaagca
    19021 gaagcctatc ttaaaaaagg ctattctcta aatgatcgtg taggaacctc ctatttggaa
    19081 aagcaatatg aagagacctt acaaggaaaa cgctcggtaa aagaaatcca tctggataaa
    19141 tatggcaata tggaaagcgt ggatacaatt gaggaaggta gtaagggaaa caatatcaaa
    19201 ctgaccattg atttggcctt ccaagatagc gtggatgctt tactgaaaag ttatttcaat
    19261 tccgagctag aaaatggtgg agccaagtat tctgaaggtg tctatgcagt cgcccttaac
    19321 ccaaaaacag gtgcggtttt gtctatgtca gggattaaac atgacttgaa aacgggagag
    19381 ttgacgcctg attccttggg aacggtaacc aatgtctttg ttccaggttc ggttgtcaag
    19441 gcggcgacca tcagctcagg ttgggaaaat ggagtcttgt caggaaacca gaccttgaca
    19501 gaccagtcca ttgtcttcca aggttcagct cccatcaatt cttggtatac tcaggcttac
    19561 ggttcattcc ctatcacagc ggtccaagct ctggagtatt catcaaatac ctatatggtc
    19621 caaacagcct taggtcttat ggggcaaacc tatcaaccca atatgtttgt cggcaccagc
    19681 aatctagagt ctgctatgga gaaactgcgt tcaacctttg gcgaatatgg cttgggtact
    19741 gcgacaggaa ttgacctacc agatgaatct actggatttg ttcccaaaga gtatagcttt
    19801 gctaattaca ttactaatgc ctttgggcag tttgataact atacgccgat gcagttggct
    19861 cagtatgtag caactattgc aaataatggt gttcgtgtgg ctcctcgtat tgttgaaggc
    19921 atttatggta ataatgataa gggaggactg ggtgacttga ttcagcaact gcaaccgaca
    19981 gagatgaata aggtcaatat atccgactcc gatatgagca tcttgcacca aggtttttat
    20041 caggttgccc atggtactag tgaattgaca actggacgtg ccttttcaaa tggcgccttg
    20101 gtatccatta gcggaaaaac aggtacagcc gaaagctatg tggcagatgg tcagcaagca
    20161 accaatacca atgcggtggc ctatgcccca tctgataatc cccaaatcgc tgttgcagtg
    20221 gtctttcctc ataataccaa tctaacaaat ggtgtaggac cttccattgc gcgtgacatt
    20281 atcaatctgt atcaaaaata ccatccaatg aactagaaag gaaattatgc tttatccaac
    20341 acctattgcc aagttgattg acagttattc taagttacca ggtatcggga ttaagacggc
    20401 tacgcgtctg gccttttata cgattgggat gtctgctgat gatgtcaatg aatttgcaaa
    20461 aaatctcctt tctgctaaga gagaattgac atattgttct atttgtggac gtttgacaga
    20521 cgacgatcct tgttctatct gtactgatcc gactcgtgac cagacaacaa ttttagttct
    20581 tgaggatagt agagatgtgg cagccatgga aaatatccaa gaataccatg gactctatca
    20641 tgtccttcat ggcctcattt ctcctatgaa tggtatcagt ccggacgata tcaatctcaa
    20701 gagccttatg actcgtctta tggatagtga ggtttcagaa gtgattgtgg cgactaatgc
    20761 tacagcggat ggtgaagcga cttccatgta tctttcacgt ttgctcaagc cggctggtat
    20821 caaggttacg cgtctagcac gaggtctcgc tgtgggagcg gacattgagt atgcggacga
    20881 agtgacactc ttacgagcga ttgaaaatcg gacagagttg taagtgtagg caaatttacg
    20941 aactccattc atttataaaa aatcaaagag gctgaaaatc gttcctatcg gcctcttttt
    21001 gtatagtgtg atgagtagga tcaggttcaa gttttaaaaa accaagcaaa tatgatatac
    21061 taaagagcga gtattctagt agaattagga caaataatat gaaacaaacg attattcttt
    21121 tatatggtgg acggagtgcg gaacgcgaag tctctgtcct ttcagctgag agtgtcatgc
    21181 gtgcggtcga ttacgaccgt ttcacagtca agactttctt tatcagtcag tcaggtgact
    21241 ttatcaaaac acaggaattt agtcatgatc cggggcaaga agaccgtctc atgaccaatg
    21301 aaaccattga ttgggataag aaagttgcac caagtgctat ctacgaagaa ggtgcagtgg
    21361 tctttccagt ccttcacggg ccaatgggag aagatggctc tgttcaagga ttcttggaag
    21421 ttttgaaaat gccttacgtt ggttgcaaca ttttgtcatc aagtcttgcc atggataaaa
    21481 tcacgactaa gcgtgttctg gaatctgctg gtattgccca agttccttat gtggctatcg
    21541 ttgaaggcga tgatgtgact gctaaaatcg ctgaagtgga agaaaaattg gcttatccag
    21601 tcttcactaa gccgtcaaac atggggtcta gtgtcggtat ttctaagtct gaaaaccaag
    21661 aagaactccg tcaagcctta gaacttgcct tccgatatga tagccgtgtc ttggttgagc
    21721 aaggagtgaa tgcccgtgaa attgaggttg gcctcttggg taactacgat gtcaagagca
    21781 cgctaccagg agaagttgtc aaggatgttg ccttttatga ctacgatgcc aagtatattg
    21841 ataacaagat tactatggat attcctgcca aaatcagtga tgatgtggtg gctgtcatgc
    21901 gtcaaaatgc agaaacagcc ttccgtgcca ttggtggcct tggtctatct cgttgcgatt
    21961 tcttctatac agataaggga gagatttttc tcaacgagct caataccatg ccaggtttca
    22021 cccagtggtc tatgtaccca ctactttggg acaatatggg gatcagctac ccagaactaa
    22081 tcgagcgttt ggttgacctt gccaaggaaa gttttgacaa gcgcgaagcg catttgatat
    22141 aaaatgaaag agagggtaga agccagaacc atcactgcaa ggtgactaga gttctcggac
    22201 ttcagccctt tttaaaggag tagaaatgaa attaacaatc catgaaattg cccaagttgt
    22261 tggagtcaaa aatgatatca gtatctttga ggacacccag ttagaaaaag ctgagtttga
    22321 tagtcgtttg attggaactg gagatttatt tgtgccactt aaaggtgcgc gtgatggcca
    22381 tgactttatt gaaacagcct ttgaaaatgg tgcagcagta accttgtctg agaaagaggt
    22441 ctcaaatcat ccttacattc tagtagatga tgttttgaca gcctttcaat ccctagcatc
    22501 ctactatctt gaaaaaacga ctgttgatgt ctttgctgtt acaggttcaa atggcaagac
    22561 aacgactaag gatatgttgg cgcatttact gtcaacaaga tacaagacct acaaaacaca
    22621 aggaaattac aataacgaga ttggccttcc ttacacagtt cttcatatgc ctgaagggac
    22681 agaaaagttg gtcttggaga tggggcagga tcacttggga gatattcatc tcttgtctga
    22741 attggctcgt ccaaaaacag ccatcgtgac cttggttgga gaagctcatt tggccttttt
    22801 caaagaccgt tctgagattg ctaagggaaa aatgcaaatt gcagacggaa tggcttcagg
    22861 ttccttgctt ttagcgccgg ctgaccctat cgtagaggac tatttaccaa ctgataaaaa
    22921 ggtggttcgt tttgggcaag gggcagagct ggaaattact gacttggttg agcgcaaaga
    22981 tagtctaacc ttcaaggcca atttcttaga gcaagccctt gatttgccag taactggcaa
    23041 gtacaatgcg acaaatgcta tgattgcatc ctatgttgcc ttgcaagaag gagtttcaga
    23101 ggagcaaatt cgtttggcct tccaagatct tgaattgacg cgtaaccgta ccgaatggaa
    23161 gaaagcagcc aatggagcag atatcctgtc tgatgtatac aatgccaatc caactgctat
    23221 gaagttgatt ttagagactt tctctgccat tccagccaat gaaggtggca agaaaattgc
    23281 ggttttagcg gacatgaagg aactgggtaa ccagtcagtt caactccata atcagatgat
    23341 tttgagcctt tctccagatg tgcttgatac cgttattttc tatggagaag atattgctga
    23401 attagcccaa ttggctagtc aaatgttccc aataggccac gtttactact tcaagaaaac
    23461 agaagaccaa gaccaatttg aagacctagt caagcaggtc aaagaaagcc ttggagccta
    23521 tgaccaaatc ttgctaaaag gttctaactc tatgaatcta gccaaattgg tagaacgttt
    23581 agaaaatgaa gtcaagtgat tttgtcaaac atttacgtat ttctaaatcc atttttaaca
    23641 actagcatgg tataataata tgcaggaaaa ttttgaatta tgaggaagac tagatgaatt
    23701 tatgggatat tttctttacg actcaggcaa ccgagccgcc caaatttgac cttttttggt
    23761 atgttagcct atttacgctc ttagccttaa ccttttatac agcccatcgc tatcgtgaaa
    23821 agaaggttta ccaacgattt ttccaaatct tgcagactgt tcagttaatc cttctttatg
    23881 gttggtactg ggtcaatcat atgccactgt cagaaagcct acccttttac cattgccgta
    23941 tggctatgtt tgtggtactc ttgcttcctg gtcaatccaa atataaacaa tactttgcat
    24001 tattgggaac atttgggaca ttagcagcct ttgtttatcc agtgccagat gcttaccctt
    24061 ttccacatat caccattcta tcctttatct ttggtcattt agcactcttg gggaactctc
    24121 tagtttatct attgagacag tataatgcgc gattgctgga tgtgaaggga atttttctca
    24181 tgacctttgc cctaaatgcc ttgatttttg tggtcaattt ggtgacaggt ggcgattacg
    24241 gatttttgac aaaaccgcca ttggttgggg atcacggtct agtagctaat tatttacttg
    24301 tttcaattgt gctggtagct actatcagtt tgactaagaa aatcttagaa ttctttttag
    24361 ctcaagaagc agaaaaaatg attgtaaagg aagcttaaca cagagctttc ttttttgctc
    24421 ttagagagtt tttacaagca gcttataaaa taagaatttc tgaatagaca aactcaaaaa
    24481 atggctggga aatttaggaa aaaagcaagc acgattaaat tttttgtgtt ataatatttt
    24541 gtgaatagct atgcctatgt ttagctatgg aataatacga agtgcgaaac ttggaagata
    24601 gagaggaagc gatgtaatgg ctagagaagg cttttttaca ggtctagata ttggaacaag
    24661 ctctgtcaag gtgcttgtgg ccgagcagag aaatggtgaa ttaaatgtaa ttggcgtgag
    24721 taatgccaaa agtaaaggtg taaaggatgg aattattgtt gatattgatg cagcagcaac
    24781 tgctatcaag tcagccattt cccaagcgga agaaaaggca ggcatttcga ttaaatcagt
    24841 gaatgtcggc ttgcctggta atcttttgca ggtagaacca actcagggga tgattccagt
    24901 aacatctgat actaaggaaa ttacggatca agatgttgaa aatgttgtca aatcagcttt
    24961 gacaaagagt atgacacctg accgtgaagt cattaccttt attcctgaag aatttattgt
    25021 ggatggtttc caagggattc gtgacccacg tggcatgatg ggggttcgcc ttgaaatgcg
    25081 tggtttgctt tatacaggac ctcgtactat cttgcacaat ttgcgtaaga cggttgagcg
    25141 tgcaggtgtt caggttgaaa atgttatcat ttcaccacta gcaatggttc agtctgtttt
    25201 gaacgaaggg gaacgtgaat ttggtgctac agtgattgat atgggggcag gtcaaacgac
    25261 tgtcgctaca atccgtaatc aagaactcca gttcacacat attctccaag aaggtggaga
    25321 ttatgtaact aaagatatct ccaaggtttt gaaaacctct cgcaaattag cggaaggctt
    25381 gaaactgaat tacggggaag cctatccgcc tcttgcaagc aaagaaacct tccaagtaga
    25441 ggttattgga gaagtggaag cagtcgaagt gacggaagcc tacttgtcag aaattatttc
    25501 tgcacgaatc aagcacatcc ttgaacaaat caagcaagaa ttagatagaa ggcgtctatt
    25561 ggacctccct ggtggtattg tcttaatcgg tgggaatgcc attttaccag gtatggttga
    25621 gcttgctcag gaagtctttg gcgtccgtgt caagctttat gttccaaatc aagttggtat
    25681 ccgtaatcca gcctttgcgc atgtgattag tttatcagaa tttgcgggtc aattaacaga
    25741 agttaatctt ttggctcagg gagcgataaa aggtgagaat gacttaagtc atcagccaat
    25801 tagttttggt gggatgctgc aaaaaacagc tcagtttgta caatcaacgc ctgttcaacc
    25861 agcacctgct ccagaagtag agccggtggc gcctacagaa ccaatggcgg atttccaaca
    25921 agcttcacaa aataaaccga aattagcaga tcgtttccgt ggcttgatcg gaagcatgtt
    25981 tgacgaataa agaggaaaaa taaattatga cattttcatt tgatacagct gctgctcaag
    26041 gggcagtgat taaagtaatt ggtgtcggtg gaggtggtgg caatgccatc aaccgtatgg
    26101 tcgacgaagg tgttacaggc gtagaattta tcgcagcaaa cacagatgta caagcattga
    26161 gtagtacaaa agctgagact gttattcagt tgggacctaa attgactcgt ggtttgggtg
    26221 caggaggtca acctgaggtt ggtcgtaaag ccgctgaaga aagcgaagaa acactgacgg
    26281 aagctattag tggtgccgat atggtcttca tcactgctgg tatgggagga ggctctggaa
    26341 ctggagctgc tcctgttatt gctcgtatcg ccaaagattt aggtgcgctt acagttggtg
    26401 ttgtaacacg tccctttggt tttgaaggaa gtaagcgtgg acaatttgct gtagaaggaa
    26461 tcaatcaact tcgtgagcat gtagacactc tattgattat ctcaaacaac aatttgcttg
    26521 aaattgttga taagaaaaca ccgcttttgg aggctcttag cgaagcggat aacgttcttc
    26581 gtcaaggtgt tcaagggatt accgatttga ttaccaatcc aggattgatt aaccttgact
    26641 ttgccgatgt gaaaacggta atggcaaaca aagggaatgc tcttatgggt attggtatcg
    26701 gtagtggaga agaacgtgtg gtagaagcgg cacgtaaggc aatctattca ccacttcttg
    26761 aaacaactat tgacggtgct gaggatgtta tcgtcaacgt tactggtggt cttgacttaa
    26821 ccttgattga ggcagaagag gcttcacaaa ttgtgaacca ggcagcaggt caaggagtga
    26881 acatctggct cggtacttca attgatgaaa gtatgcgtga tgaaattcgt gtaacagttg
    26941 ttgcaacggg tgttcgtcaa gaccgcgtag aaaaggttgt ggctccacaa gctagatctg
    27001 ctactaacta ccgtgagaca gtgaaaccag ctcattcaca tggctttgat cgtcattttg
    27061 atatggcaga aacagttgaa ttgccaaaac aaaatccacg tcgtttggaa ccaactcagg
    27121 catctgcttt tggtgattgg gatcttcgcc gtgaatcgat tgttcgtaca acagattcag
    27181 tcgtttctcc agtcgagcgc tttgaagccc caatttcaca agatgaagat gaattggata
    27241 cacctccatt tttcaaaaat cgttaagtaa atgaatgtaa aagaaaatac agaacttgtt
    27301 tttcgagaag ttgcagaggc tagtctgagt gctcatcgag agagtggttc ggtctctgtc
    27361 attgcagtta ccaagtatgt agatgtaccg acagcggaag ccttgcttcc gctaggtgtc
    27421 catcatatcg gtgaaaatcg tgtagataag tttctggaaa aatatgaagc tttaaaagat
    27481 cgagatgtga cttggcattt gattggtacc ttgcaaagac gtaaggtgaa agatgtcatt
    27541 caatacgttg attatttcca tgcattggac tcagtaaagc tagcagggga aattcaaaaa
    27601 agaagtgacc gagtcatcaa gtgtttcctt caagtaaata tttctaaaga agaaagcaaa
    27661 cacggttttt cgagagagga actgctggaa atcttgccag agttagccag actagataag
    27721 attgaatatg ttggtttaat gacgatggca ccttttgagg ctagcagtga gcagttgaaa
    27781 gagattttca aggcggccca agatttacaa agagaaattc aagagaaaca aattccaaat
    27841 atgcctatga ccgagttaag tatgggaatg agtcgtgatt ataaagaagc gattcaattc
    27901 ggttccactt ttgttcgtat aggtacatca ttttttaagt aggagagaac catgtcttta
    27961 aaagatagat tcgatagatt tatagattat tttacggagg atgaggattc aagtctccct
    28021 tatgaaaaaa gagatgagcc tgtgtttact tcagtaaatt cttcacagga accggctctc
    28081 ccaatgaatc aaccttcaca gtcggctggg acaaaagaga acaatatcac cagacttcat
    28141 gcaagacaac aggaattggc aaatcagagt cagcgtgcaa cggataaggt cattatagat
    28201 gttcgttatc ctagaaaata tgaggatgca acagaaattg ttgatttatt ggcaggaaac
    28261 gaaagtatct tgattgattt tcagtatatg acagaggtgc aggctcgtcg ttgtttggac
    28321 tatttggatg gagcttgtca tgttttagct ggaaatttga aaaaggtagc ttctaccatg
    28381 tatttgttga caccagtgaa cgttattgta aatgttgaag atatccgttt accagatgaa
    28441 gatcaacagg gtgagttcgg ttttgatatg aagcgaaata gagtacgata atgatttttt
    28501 taattcgtat gatttataat gcagtggata tttactccct aattttggta gccttcgctg
    28561 tcatgtcttg gtttccaggt gcctacgaat ccagtttagg tcgttggatt gtagcgttgg
    28621 taaaaccagt gcttgctccc ttgcaacgcc tgcctttaca gatagcgggt cttgatttat
    28681 ctgtttgggt cgcgattgtt ttggttcgat ttttaggaga aaacctagtg cgttttctgg
    28741 cgatgatagg atgaataaag ggatttatca gcatttctcc atagaagatc gtccatttct
    28801 tgacaaggga atggaatgga taaagaaggt agaagatagc tatgctcctt ttttaactcc
    28861 ttttatcaat cctcatcagg agaagctatt aaagattttg gccaaaacct atggtcttgc
    28921 ttgtagcagt agtggggaat tcgtctcgag tgagtatgtt cgagttttat tatacccaga
    28981 ttatttccaa ccagagtttt cagattttga aatatctctc caggaaattg tgtattccaa
    29041 taaatttgaa catttaacgc atgctaagat tttagggaca gtcatcaatc aattagggat
    29101 tgaacggaaa ctttttggag atatcctagt agatgaagaa cgggcgcaga ttatgattaa
    29161 tcagcagttt cttcttctct ttcaagatgg actaaagaaa attggtcgta tacctgtttc
    29221 gctggaggaa cgtcctttca ccgagaaaat agataagcta gaacagtatc gagaactgga
    29281 tttatctgtg tctagttttc gattagatgt tcttttatca aatgttttga aactatctag
    29341 gaatcaagca aaccagttga ttgaaaagaa acttgtccaa gtaaattatc atgtggtaga
    29401 caaatcagat tacactgttc aagttggaga cttgattagt gtgagaaaat ttggtcgctt
    29461 gagattactt caagataagg gacaaacgaa aaaagagaag aaaaaaataa ccgtccagtt
    29521 attattaagt aagtgaggaa tagaatgcca attacatcat tagaaataaa ggacaagact
    29581 tttggaactc gattcagagg ttttgatcca gaagaagtcg atgaattttt agatattgtg
    29641 gttcgtgatt acgaagatct tgtgcgtgcg aatcatgata aaaatttgcg tattaagagt
    29701 ttagaagagc gtttgtctta ctttgatgaa ataaaagatt cattgagcca gtctgtattg
    29761 attgctcagg atacagctga gagagtgaaa caggcggcgc atgaacgttc aaacaatatc
    29821 attcatcaag cagagcaaga tgcgcaacgc ttgttggaag aagctaaata taaggcaaac
    29881 gagattcttc gtcaagcaac tgataatgct aagaaagtcg ctgttgaaac agaagaattg
    29941 aagaacaaga gccgtgtctt ccaccaacgt ctcaaatcta caattgagag tcagttggct
    30001 attgttgaat cttcagattg ggaagatatt ctccgtccaa cagctactta tcttcaaacc
    30061 agtgatgaag cctttaaaga agtggttagc gaagtacttg gagaaccgat tccagctcca
    30121 attgaagaag aaccaattga tatgacacgt cagttctctc aagcagaaat ggaagaatta
    30181 caagctcgta ttgaggtagc cgataaagaa ttgtctgaat ttgaagctca gattaaacag
    30241 gaagtggaaa ctccaactcc tgtagtgagt cctcaagttg aagaagagcc tctgctcatc
    30301 cagttggccc aatgtatgaa gaaccagaag tagctccagt gcatccgaca ggtccaacac
    30361 cagctacaga aactgttgat tcagcaccgg gatttgaagc accgcaagaa tctgttacaa
    30421 ttttataaga aatattctga gaacaatatc ttatccttat attcccagcg agcaggagat
    30481 ggtgtgagtc ctgcattccc tatcgataag attatcctct caaaaactca agtctgaatc
    30541 tagtaagatt tgacgttccc cacgttacgg gataagaggg agaaagacta aaactttttc
    30601 cgaataaagg tggtaccacg attttcgtcc tttttggaag tcgtggtttt taatttgtta
    30661 ttatttataa aggagatacc atgaaactca aagacaccct taatcttggg aaaactgaat
    30721 tcccaatgcg tgcaggcctt cctaccaaag agccagtttg gcaaaaggaa tgggaagatg
    30781 caaaacttta tcaacgtcgt caagaattga accaaggaaa acctcatttc accttgcatg
    30841 atggccctcc atacgctaac ggaaatatcc acgttggaca tgctatgaac aagatttcaa
    30901 aagatatcat tgttcgttct aagtctatgt caggatttta cgcaccattt attcctggtt
    30961 gggatactca tggtctgcca atcgagcaag tcttgtcaaa acaaggtgtc aaacgtaaag
    31021 aaatggactt ggttgagtac ttgaaacttt gccgtgagta cgctctttct caagtagata
    31081 aacaacgtga agattttaaa cgtttgggtg tttctggtga ctgggaaaat ccatatgtga
    31141 ccttgactcc tgactatgaa gcagctcaaa ttcgtgtatt tggtgagatg gctaataagg
    31201 gttatatcta ccgtggtgcc aagccagttt actggtcatg gtcatctgag tcagcccttg
    31261 ctgaagcaga gattgaatac catgacttgg tttcaacttc cctttactat gccaacaagg
    31321 taaaagatgg caaaggagtt ctagatacag atacttatat cgttgtctgg acaacgactc
    31381 catttaccat cacagcttct cgtggtttga cggttggtgc agatattgat tacgttttgg
    31441 ttcaacctgc tggtgaagct cgtaagtttg tcgttgctgc tgaattattg actagcttgt
    31501 ctgagaaatt tggctgggct gatgttcaag ttttggaaac ttaccgtggc caagaactca
    31561 accacatcgt aacagaacac ccatgggata cagctgtaga agagttggta attcttggtg
    31621 accacgttac gactgactct ggtacaggta ttgtccatac agcccctggt tttggtgagg
    31681 acgattacaa tgttggtatt gctaataatc ttgaagtcgc agtgactgtt gatgaacgtg
    31741 gtatcatgat gaagaatgct ggtcctgaat ttgaaggtca attctatgaa aaggtagttc
    31801 caactgttat tgaaaaactt ggtaacctcc ttcttgccca agaagaaatc tctcactcat
    31861 atccatttga ctggcgtact aagaaaccaa tcatctggcg tgcagttcca caatggtttg
    31921 cctcagtttc taaattccgt caagaaatct tggacgaaat tgaaaaagtg aaattccact
    31981 cagaatgggg taaagtccgt ctttacaata tgatccgtga ccgtggtgac tgggttatct
    32041 ctcgtcaacg tgcttggggt gttccacttc ctatcttcta cgctgaagat ggtacagcta
    32101 tcatggtagc tgaaactatt gaacacgtag ctcaactttt tgaagaacat ggttcaagca
    32161 tttggtggga acgtgatgcc aaagacctct tgccagaagg atttactcat ccaggttcac
    32221 caaacggcga gttcaaaaaa gaaactgata tcatggacgt ttggtttgac tcaggttcat
    32281 catggaatgg agtggtggta aaccgttctg aattgactta cccagccgac ctttacctag
    32341 aaggttctga ccaataccgt ggttggttta actcatcact tatcacatct gttgccaacc
    32401 atggcgtagc accttacaaa caaatcttgt cacaaggttt tgcccttgat ggtaaaggtg
    32461 agaagatgtc taaatctctt ggaaatacca ttgctccaag cgatgttgaa aaacaattcg
    32521 gtgctgaaat cttgcgtctc tgggtaacaa gtgttgactc aagcaatgac gtgcgtatct
    32581 ctatggatat cttgagccaa gtttctgaaa cttaccgtaa gattcgtaac actcttcgtt
    32641 tcttgattgc caatacatct gactttaacc cagctcaaga tacagtcgct tacgatgagc
    32701 ttcgttcagt tgataagtac atgacgattc gctttaacca gcttgtcaag accattcgtg
    32761 atgcctatgc agactttgaa ttcttgacga tctacaaggc cttggtgaac tttatcaacg
    32821 ttgacttgtc agccttctac cttgattttg ccaaagatgt tgtttacatt gaaggtgcca
    32881 aatcactgga acgccgtcaa atgcagactg tcttctatga cattcttgtc aaaatcacca
    32941 aactcttgac accaatcctt cctcacactg cggaagaaat ctggtcatat cttgagtttg
    33001 aaacagaaga cttcgtccaa ttgtcagaat taccagaagt tcaaactttt gctaaccaag
    33061 aagaaatctt ggatacatgg gcagccttca tggactttcg tggacaagca caaaaagcct
    33121 tggaagaagc tcgtaatgca aaagttatcg gtaaatcact tgaagcacac ttgacagttt
    33181 atccaaatga agttgtgaaa actctactcg aagcagtaaa cagcaatgta gcacaacttt
    33241 tgatcgtgtc tgagttgacc atcgcagaag gaccagctcc agaagctgcc cttagcttcg
    33301 aagatgtagc cttcacagtt gaacgtgctg ctggtgaagt atgtgaccgt tgccgtcgta
    33361 tcgacccaac aacagcagaa cgcagctacc aggcagttat ctgtgaccac tgtgcaagca
    33421 tcgtagaaga aaactttgcg gaagcagtcg cagaaggatt tgaagagaaa taagattgaa
    33481 aagtctaggc aaaattcaat ttgagaagaa aagacaacta attttatagt ctattaaacg
    33541 cattgtatca cgtttttgaa cacctgatat gatgcgtttt ttatttattt taaaaatttg
    33601 cgaggtatga ctttttatac tcaacaagaa tcaaagagaa acttagcaag ctaacagtag
    33661 taagataaaa taggaatttg atattaggga taagattggt aaatagtgta atatttttac
    33721 aacaataaat ttatatagtt atttctggtt tctgaaaagt attatatttt atttcatatt
    33781 atacaaattt ttattttata atatcagaac atactttttt taaaagcaaa tatgatacaa
    33841 ttttatttga aaaaaataaa aaaggagatt ttattataaa attaaaaaga cttgctttaa
    33901 ttagtggtat cgtcggtctt gtgggaggaa ttttacttct tattggtcct tttgtcttgt
    33961 tgggaatagc ggtaaacaca gctgctacaa ctcttaatgg aggagctact gcaggggctt
    34021 tttcaggtgt agccttactc ttgaatgcct tgaagattgc aaatcttgtt cttggtatca
    34081 ttgctattgt ttactataaa ggagataagc gtgtaggtgc agctccgtct gtactaatga
    34141 ttgtttctgg tggagttagt ctcattctat tccgttctta ggatgggttg gggggattct
    34201 tgctattatc ggaggatctc tattcctttc aacattgaag aaattcaaat cagaagaata
    34261 aaaggtattt tagcatgaaa agaacaaaaa agtttatcgg tataggagta gctctattat
    34321 ctctttctct tctagttgca tgtggaacat aaagttcaaa gaatacttca acaagtaatg
    34381 atgagaagac agtagcaaca tccaatagtt caaaagaaac aatcactttc gatacaccgg
    34441 ttgtaacaga cgatgcgatt gaatcaatac gcacttatgc agattatata gatctttata
    34501 aaaatatttt tgatgattat tttactaaag ctgaggaagg tttcaaaggc acagctatgg
    34561 aaaataatga ctcgtttact aaactaaaag agtcaactca aaaattattc gatgcgcaga
    34621 aaaaaaggtt aaataatgaa gatagaatag aaacagccaa aaacaatgtg attgccaaac
    34681 attgtcaaac agtcctttcc tttttggttt tgactagctt ttttgtgaaa aattgtgtaa
    34741 aatagaatag ataaacgagg ggaaacctcg gaaaatttaa aggagaatcc atctaatggt
    34801 aaaattggtt tttgctcgcc acggtgagtc tgaatggaac aaagctaacc ttttcactgg
    34861 ttgggctgat gttgatttgt ctgaaaaagg tacacaacaa gcgattgacg ctggtaaatt
    34921 gatcaaagaa gctggtatcg aatttgacca agcttacact tcagtattga aacgtgctat
    34981 caaaacaact aacttggctc ttgaagcttc tgaccaattg tgggttccag ttgaaaaatc
    35041 atggcgcttg aacgaacgtc actacggtgg tttgactggt aaaaacaaag ctgaagctgc
    35101 tgaacaattt ggtgatgagc aagttcacat ctggcgtcgt tcatacgatg tattgcctcc
    35161 aaacatggac cgtgatgatg agcactcagc tcacacagac cgtcgttacg cttcacttga
    35221 cgactcagtt atcccagatg ctgaaaactt gaaagtgact ttggaacgtg ctcttccatt
    35281 ctgggaagat aaaatcgctc cagctcttaa agatggtaaa aacgtattcg taggagctca
    35341 cggtaactca atccgtgccc ttgtaaaaca catcaaaggt ttgtcagatg atgagatcat
    35401 ggacgtggaa atccctaact tcccaccatt ggtattcgaa ttcgacgaaa aattgaacgt
    35461 tgtttctgaa tactaccttg gaaaataaaa aattgtaagc ctaggattga tttctaggct
    35521 ttttatgtta gtatggaagt atgataagga ataaaaaaca agattatgta ctggcctaca
    35581 aacaaccagc ttcaaccact tacatgggtt gggaagaaga agctttaccg ataggtaatg
    35641 gttctttagg agcaaaagta tttggcctta taggggctga acggattcaa tttaatgaaa
    35701 aaagtctctg gtctggaggt ccacttcctg atagttcaga ttatcagggt ggaaatcttc
    35761 aggatcagta tgttttttta gctgagattc ggtaggcttt ggagaagaga gattacaatc
    35821 tggctaagga actggctgag cagcacctaa ttgggccaaa aacgagtcaa tatgggacct
    35881 atctgtcttt tggggatatt cacattgagt tcagccagca aggtacgact ttgtctcagg
    35941 tgacggacta tcagagacag ctgaatatta gtaaggcact tgcgacgact tcttatgtct
    36001 ataagggaac gcgatttgaa cgtgaagctt ttgcgagttt tccagatgat ctcttggttc
    36061 aatgttttac taaggaaggg ttggaaactc tagattttac tatagaacta tccttgacct
    36121 gtgatttggc ttctgatgga aagtatgagc aggaaaaatc tgattacaag gagtgtaagt
    36181 tggatattac tgattctcat atcttgatga agggaagagt taaggataat gatctgcggt
    36241 ttgctagtta tctagcttgg gaaacggatg gagatattag agtttggtca gatagggttc
    36301 agatatcagg agccagttat gccaatctct tcttggccgc taagacggat tttgcccaaa
    36361 atcctgctag caattatcgc aagaaactag atttagagca acaggtgata gacttggtgg
    36421 acacagctaa agaaaagggc tatacccaat tgaaatcaag gcatatcgag gactaccaag
    36481 ccttattcca gcgtgttcaa ttggatttgg aagctgatgt tgacgcatcc actacagatg
    36541 atttgttaaa aaattataag ccacaagaag ggcaggcttt ggaggagctg ttcttccagt
    36601 atggacggta tttattgatt agttcgtcca gagactgccc agatgctcta ccagctaacc
    36661 tacagggagt ctggaatgcg gtcgacaatc ctccttggaa ttcggactat cacttaaatg
    36721 tcaatctgca gatgaattat tggccagcct atgttaccaa tctcctagag acggtctttc
    36781 cagtcatcaa ctatgtagat gatttgcgtg tctatggtcg tctagcggct gtaaagtatg
    36841 caggaatcgt ctctcagaaa ggtgaggaga atggttggtt ggttcatact caagcgactc
    36901 cctttggttg gacggcacct ggttgggatt actattgggg ttggtcacca gctgccaatg
    36961 cgtggatgat gcaaaccgtt tatgaagcct attcatttta tagggaccaa gactatctca
    37021 gggagaaaat ttatcccatg ttgagggaaa cggttcgttt ttggaatgcc tttttacata
    37081 aggatcagca ggcgcagcgt tgggtgtctt ctccgtctta ttccccagaa catgggccga
    37141 tttcgattgg caatacctat gaccaatctc tgatttggca gttatttcat gattttattc
    37201 aggctgctca ggaattggga ctggatgagg acttgttgac tgaggttaag gagaagtctg
    37261 atttactaaa tcctttgcaa atcactcaat ctggtcgaat cagggagtgg tatgaggagg
    37321 aagagcagta ttttcaaaat gagaaagtgg aggcccagca tcggcacgct tcccatctag
    37381 tgggactcta tcctggcaat ctctttagct acaagggaca agagtatatt gaagcggcgc
    37441 gtgctagcct caatgatcgt ggagatggcg gcacaggctg gtccaaggct aataagatca
    37501 atctctgggc gcgtttggga gatggcaatc gagcccataa attattggca gagcagttaa
    37561 agacatccac cttgcaaaat ctttggtgta gccatcctcc ttttcagata gatggtaatt
    37621 ttggtgctac tagtggcatg gcagaaatgt tactccagtc tcatgcagct tatctggtac
    37681 ctctagctgc cctacctgat gcttggtcaa caggttctgt ttcaggctta atggcacgtg
    37741 gacattttga agtgagcatg agctgggaag ataaaaaact cttacagttg accattttat
    37801 caaggagtgg aggagatttg cgagtttctt atccagatat tgagaagagt gtgattaaaa
    37861 tgaatcaaga aaaaataaaa gcgaaatgca tggggaaaga ttgtatttcg gtggcaacag
    37921 cagaaggtga tcttgttcaa ttttattttt aagaagatgt tataaggcag taatttgaaa
    37981 ctgcctttta ataaggattt aagaatataa gcagttttca actagttgaa aaaacgttat
    38041 aatgataata ggaagtaata ctcaatgaaa atcaaagagc acaaactagg aagctagccg
    38101 caggttgctc aaaacagtgt tttgaggttg cagatggaag ctgacgtggt ttgaagagag
    38161 attttcgagg agtataattt gtttgataga gggtgggtct gatggcttat attgagatga
    38221 aacactgtta caagcgttat caggttgggg acacggagat tgtggccaat tgtgatgtga
    38281 attttgagat tgaaaagggg gagctggtta ttatccttgg tgcttcaggt gcaggcaagt
    38341 caacagttct taaccttctt gggggaatgg ataccaatga tgaaggggaa atctggattg
    38401 atggtgttaa tattgcggat tatagttccc accagcgcac caattaccgt agaaatgatg
    38461 tggggtttgt ttttcagttt tataatctag tttctaatct gacagctaag gaaaatgtgg
    38521 aactggcttc tgaaattgtg acagatgcct tgaatcctga tcaagtcttg acagatgtag
    38581 gtctggctca tcgtctcaat aactttccag cccagctttc tggaggggag caacagcgag
    38641 tctccattgc acgcgcggta gccaaaaatc ctaaaattct cctttgtgat gaaccgactg
    38701 gagccttgga ttatcagacg ggcaagcagg ttttgaaaat tctccaagac atgtctcgtc
    38761 aaaagggagc gacggtgatc atcgtgactc ataatggagc tttggcgccc attgctgatc
    38821 gcgtgattca aatgcacgat gccagtgtca aggatgtggt gctcaaccag catcctcagg
    38881 atattgacag tttggagtac tagcatgatc aagcgaaaaa cttattggaa ggacttagtt
    38941 cagtccttca caggctccaa ggggcgtttt ttatccatct tgatcctgat gatgttggga
    39001 tctctagcct tagtaggcct caaagtaacc agtcccaaca tggaggcgac agctaatgct
    39061 tatttaacaa ctgctcaaac cttggatttg gcagtcatgt ctaactatgg cttggatcaa
    39121 gcagaccaag aagaactaaa acagacggag ggcgcagagg tcgagtttgg ctatttgaca
    39181 gatgtgacta tggataatgg gcaggatgcc attcggctgt actccaaacc agagcgaatt
    39241 tcaacctttc agctaagaaa gggacgactt cctcagtcag acaaggaaat cgctttggcc
    39301 actcatttgc aaggccaata cagcgtggga caggagatta gttttaaaga aaaagaagag
    39361 ggtcattcct ctttaaaaga ccatacttat accattactg gttttgtgga ttcggctgaa
    39421 atcctctccc agcgagatat gggctacgca ggaagtggaa gtgggactct gacagcctat
    39481 ggggtgattt tacctagtca gtttgatcag aaagtctaca atatagctcg tttgaaatat
    39541 caagatttag caggtttaaa tgccttttca tcagcttatg aagaaaaatc caagcaacat
    39601 caggaagacc ttgaacaaac tttatcagat aatggcaagg tacgtctgca acttttgaaa
    39661 aaagaaggac aagagtctct agacaagggg caagagaccc ttgacaaggc tcagactaat
    39721 ttgcaggaag gcaagcgtcg tttagcagct gctcaagctc gtatacaggc tcaagaaagt
    39781 caactagcct tgtttcctca agttcagaga gagcaggcta gtgctcaact tacccaagcc
    39841 aagcaggaat tgggcaagga agaggataaa ctaaagcaag ctgaacaaaa tctagcccaa
    39901 gaaaaggaaa aattagaaaa acatcagcaa gtcttggacg atttggcgga gccaaggtat
    39961 caggtctata atcgtcagac catgccaggt ggtcagggct atcttatgta tagcaatgct
    40021 tcatccagta ttcgagcagt gggcaatatc tttcctgtgg tactttatgc cgtagcatcc
    40081 atggtgacct ttacgaccat gactcgcttt gtagacgaag agcgaactca tgcagggatt
    40141 tttaaggcct tgggttaccg tagtaaggat attatcacca agtttctcct ttatggacta
    40201 gtagctggga ctgtcggaac ggctctaggt agtatacttg gtcattattt gctagccagt
    40261 gtaatttcaa gtgtcattac aaaaggcatg gtggtgggag aaacccagat tcagttctat
    40321 tggacctata gcttactagc tcttgtcttg agcttgttgg cgagtgtgtt accagcctat
    40381 ctggtggctt ggagggaact tcatgccgaa gcagcccagc ttctacttcc taaacctcct
    40441 gtcaaaggag ctaaaatctt attggagcgt atcggtttta tctggcgtcg tctcagtttt
    40501 attcataagg taacagcccg caacatcttt cgttataagc agagaatgtt gatgacaatc
    40561 tttggtgtgg caggttctgt agctctgctc tttgcaggtt tgggaatcca atcttctgta
    40621 gcaggagttc cgtctaaaca gtttcaacaa atccaacagt atcagatgct tgtctctgaa
    40681 aatcctagtg cgaccaatca ggacaaggta gagctagcag aagtattgaa agggcaggag
    40741 atactagcct accagaaaat ctattctaaa acgctagaca aggatttcaa aggcaaggct
    40801 ggtcttcaaa acattactct tatgatgata gagaaggaag atttgactcc ctttatccat
    40861 cttcaacatc atcagcagga gctgacatta aaagatggca tcgttattac agctaaactc
    40921 gcccagctgg caggtgtcaa ggttgggcag actttagaaa ttgaaggtaa ggaactaaag
    40981 gtcgctgcta ttactgagaa ctacgttggt cactttattt atatgagtca ggctagctat
    41041 gagcaacttt acggacagct accccaagcc aacacttatc tggtctcatt aagggatacc
    41101 agtgcaacta gtatcgaaag tcaggcgggc ttgcttatga atcaatctgc ggtgtccagc
    41161 gttgtccaaa atgcttcagc cattcgactc ttcgactcta tcgctagctc actcaatcag
    41221 accatgacca tcttggtcat cgtatcggtt ctattagcta ttgtcatcct ttacaatctg
    41281 accaatatca acgtagctga gagaatccgt gaactctcca ctaccaaggt tcttggtttt
    41341 cataataatg aagtcaccct ctacatttac cgtgagacga ttgtgctatc ccttgtggga
    41401 atcgtacttg gtctggtagc tggtttctat ttacaccaat ttttgattca aatgatttcg
    41461 cctgcgacta ttctctttta tccgcaggta ggctgggaag tctatgtaat cccagtggca
    41521 gcagtaagca tcattttgac cttgcttggt ttcttcgtca attattatct gagaaaggtt
    41581 gatatgttag aagccctgaa atctgtagag taaggtagtt atttttagct gattgaactt
    41641 ctatttacta atattcaaaa atcctccgtt tcaaagagca gggaactctt tgtgacagag
    41701 gattttttct atagggcttt agcagctgca attgcggctt cgaagtttgg ctcagaattg
    41761 atattatcca cgtattcaac gtagcgaatc gtattgtcag tatcgaggac aaagactgcg
    41821 cgtgctaata ggtgccattc gttgatcaag agggcataat cgcgcccgaa agaatggtca
    41881 aagtagtctg aaagcataat ggcattgtca aggtcttcag caccgcacca acgtttttga
    41941 gcaaaaggta ggtccattga aacagtcaat acgaccgtgt tgtccagtcc agccaattct
    42001 tcattaaaac gacgtgtttg agttgagcag atgcctgtat cgatagaagg aacgacactc
    42061 aagacttttt tcttgccatc aaaatcagcc agagattttt tagaaagatc tgttgtagta
    42121 agagaaaaat caagcgcctt gtcgccgact tgtagttgtt tacctgtaaa gctcacagga
    42181 tttccgagaa aagttaccat aggatactcc aatctttttt cttccattgt atctgaaaca
    42241 gtcagaattt tccaatgatt tgaccggaaa tgtgggcata gaaaaaacgc cagctcacat
    42301 gagaatgacg tttttcagag gcttattttg ccaatccttc agcaatcttg tcaaggttgt
    42361 atttcatcat gctgtagtag ctgtcgcctt ctttaccttg ttctgcgata gagtcagtaa
    42421 agatttgtgc gtagattggg atgtttgtgt cttgagaaac agttttcatt ggacggtcat
    42481 ccacacttga ttctacaaag agtgatggaa cttttgtttg gcgaagtttt tcaaccaagg
    42541 tcttgatttg ttcaggagtt ccttcttctt cagtattgat ttcccagatg taggcacttg
    42601 gaacaccata ggctttagag aagtatttga atgctccttc gctggttaca atgagtttct
    42661 tttcagcagg gatcttatta aatttatcct tactttcttt atcaagtttg tctaacttat
    42721 cagtatattc tttgagattt ttttcataga attctttatt gttagggtct ttggcgctca
    42781 attgtttggc gatattttta gcaaaaataa taccgttttc aaggttaagc caagcgtgtg
    42841 ggtcttcttt tcctttttca ttttgacctt caaggtagat aacatcaacg ccgtcgctga
    42901 ctgcgaagta gtctttgttt tcagttttct tggcattttc taccaatttt gtaaaccaag
    42961 cattgccacc tgtttcaagg ttgataccgt tatagaaaat caaatcagcc tcagaagttt
    43021 tcttaacgtc ttcaggaagt ggttcgtatt cgtgtgggtc ttgcccaatc ggaacgatac
    43081 tatgaaggtc aattttgtca ccagcaatat ttttagtaat atcagcgatg attgagtttg
    43141 tagcaacaac ttttagtttt tgaccagaag ttgtatcttt ttttccgcta gcacatgcta
    43201 caagaatgat tgcagaaaga aagagaacga gtaatgtacc taattttttc attagatcct
    43261 ccaatttatt agggctttgc cccttatttt aacaaatgtt tatttttcag tttcaaatat
    43321 cgttgtttgg gagcgataaa gaagctaatg agaaagaaac tagcagctgt aagcacgata
    43381 ctagaacctg ccgcaacatt aaaactatag ccaataaaga gtcccaaaac tgaagcagta
    43441 gctccgaagg ttgaggaaag gaaaatcata cttttcagac tattagcata cagataagca
    43501 gttgcagctg gggtaatcag catggctaca atcaggatag ttccgacact ttgcatggct
    43561 gtcacagaca cgagagtcag gagtaccatg agaaggtagt gatagaaatt gacaggcatt
    43621 cccatggctt tagccaagag ttcatcaaag gaagttatca agagttgctt gaagaaaatc
    43681 cagattaaca agaggatggc tgcccccaca cccatagtaa taaacatatc cgtatcttgg
    43741 acggccagga tattaccaaa aaggatatgg aaaaggtcag ttgaactttt agcgacacca
    43801 atcaagatga taccgagggc taagaaagaa gaaaaggtaa tgccgatggc ggtatcgctt
    43861 ttgataatcg agtttccttt gatgtaggta atgatgatgg cagctagcaa tccaaagaca
    43921 atggctccga taaagaagtc aaggcccaag atgaaggaga gggctacacc tggtaagaca
    43981 gcatgtgaaa tggcatctcc catgagtgac atcccgcgta gaataatgaa acatcccaca
    44041 gctccagcta caatcccgac gacaatagct gttatcaagg cattttgtag gaaatggaat
    44101 ttttgcaatc catcgataaa ttctgcaatc ataggtcacc tccattgaaa aagagttgat
    44161 taccgtaagc ttcttttaga ttggtttcgg taaaagtttc ttttgttgga ccaaaggcaa
    44221 tcacttctcg attgacaagt aagacttgat cgaagtagtg gggaatcttg ctgaggtcgt
    44281 ggtgaacgat gagaaccgtc ttcccagctt ttttcaaatc tctcagcgta ttcatgatga
    44341 tttcctcact gacagagtca atcccagcaa agggttcatc caagaggata tagtcggctt
    44401 cctgcaccaa acatctggca atcaagaccc gctggaattg acctccagac agttgactaa
    44461 tttgacgttc agcgtagtca gctaggccga cgatttcaag ggcctcttgc actttcttcc
    44521 aatgtttagc ctttaaactt cgaaagagag gaatagaggg aaatagtcct aacgagacgc
    44581 attccttgac cttgatggga aagttgtagt cgatattgat tttttgttcg acataggcaa
    44641 ttcggtgtaa ggatttttta acttccttgt catcgagaaa tgcctgacct tgatgtggga
    44701 taattcccag catacctttt aatagtgttg atttcccagc gccgtttgga ccaatgatgc
    44761 cggtaattgt tggtccatgg agcactagtg aaatatcctt aagtgccaac gtttctttgt
    44821 aggagacact gaggttttcg atacgtatca taaacttgta ttcctcctgt ctcttaatat
    44881 acattaaaaa aaaaattaag tcaagttaat ttttgaaaaa attaaaataa taactgaaaa
    44941 atagattcta aagataactt tcaggataaa tttctaaatt ataaaacgca tagtatgcat
    45001 agtatcaagt gtaaaaaact tggaattatg cgttttatca tggaaagatt ctttataata
    45061 gctaaaaaat aatagttaaa tggttactaa ttgcattccc tagtgatttt tgttaagata
    45121 aaggcaaata caaatgaaag cgagaacaag atgacacgtt atcaagatga tttttatgat
    45181 gctatcaatg gagaatggca acagacagct gaaatcccag cagataagtc tcaaacagga
    45241 ggttttgttg atttagacca ggaaattgaa gacttgatgc tggcgacaac agacaagtgg
    45301 ttagcaggtg aagaagtgcc tgaggatgct atcttggaaa actttgtcaa ataccaccgc
    45361 ctagttcgtg attttgacaa gagagaagct gacggtatca cacctatctt atcactcctt
    45421 aaagaatttc aagaattgga gacttttgcg gattttatag ctaaactagc agagtttgag
    45481 cttgcaggaa aaccaaactt ccttcctttt ggtgtatcgc cagactttat ggatgctaga
    45541 atcaatgttc tatgggctag cgctccaagc acaatcttgc cagatacgac ctactatgca
    45601 gaagaacatc ctcagcgcga agagctcttg actctttgga aagaaagcag cgcaaatctc
    45661 ctcaaggctt atgatttctc tgatgaagaa attgaagact tgctagaaaa aagacttgaa
    45721 ttggaccgcc gagttgcggc agtggtgctc tctaatgaag aaagttcaga atatgctaaa
    45781 ctctatcatc catattctta cgaagatttc aagaaattcg cgcctgccct acctttggat
    45841 gacttcttca aagcagttat tgggcaatta ccagacaagg ttattgtaga cgaggaacgt
    45901 ttctggcaag cagcagagca attctacagt gaggaagcct ggtctctcct taaagcaacc
    45961 ttgattttga gtgttgtcaa tctttcaacc agctatttaa cagaggatat ccgtgttttg
    46021 tctggtgcct acagccgtgc cctttctgga gttccagagg caaaagataa ggtcaaagca
    46081 gcttatcatc tagcacaaga acctttcaag caagccctgg gtctttggta cgcccgtgag
    46141 aagttctctc cagaagccaa ggcggatgtg gagaaaaaag tggcaaccat gattgatgtc
    46201 tataaggagc gtctgcttaa gaatgactgg ctcactccag aaacctgtaa acaggctatc
    46261 gtgaagctca atgtgatcaa accttatatt ggctatccag aagaattgcc tgcacgttac
    46321 aaggataagg tagtgaatga aactgccagt ctttttgaga atgctctagc ctttgcgcgt
    46381 gtggaaatca agcacagttg gagtaagtgg aaccagcctg tagattataa ggaatggggc
    46441 atgcctgctc atatggtcaa tgcctactac aatcctcaga agaacctgat tgtctttcca
    46501 gcggccattt tacaggcgcc tttctatgac ttgcatcagt catcttctgc taactacggt
    46561 ggtattgggg cagtgattgc ccatgaaatt tcccacgcct ttgatactaa cggggcttcc
    46621 tttgacgaaa atggtagcct caaggattgg tggacagaga gcgactatgc tgccttcaag
    46681 gagaaaacac aaaaagtcat tgaccaattt gatggacagg attcttatgg agcaaccatt
    46741 aacggtaaat tgactgtatc agaaaacgtg gctgacttgg gaggaatcgc agcagcgctt
    46801 gaagcagcta agagagaagc agacttctca gcagaagagt tcttctacaa cttcggtcgc
    46861 atctggcgca tgaaaggtcg accagaattt atgaaacttt tggctagcgt cgatgtgcac
    46921 gcaccagcca aactccgtgt caatgtgcaa gtaccaaact tcgacgattt ctttacaacc
    46981 tatgatgtca aagaaggaga cggaatgtgg cgttcaccag aggagcgcgt gattatttgg
    47041 taatacaaaa aatctagtca tcagaaaaaa ggcatccaat cacgtgtgaa aaccttgata
    47101 ggatgccttt tgtaatagaa agatttgctg gatagtttac ttaaatttcg attgtcatca
    47161 tcttgctaga aaaagggatt gaaggccttt tcgtgagcga tagtagtagc tggaccatgt
    47221 cctggataga catcgtagtt tggtagggtg aagagttggg tctggatact atgaaggagt
    47281 tgctccatgc taccagtcgg aaggtcggtc cgtccgatag tttcgcggaa tagagcatct
    47341 cccgtcaaga ctaggtgagc atcaggaaag actagggaaa caccaccgat agagtgccct
    47401 ggggtcggta gaaccttaaa acgaaattcc tctagttggt attcttcgtg aaagacaaag
    47461 gtgtgttctg caggttttgt gaccacatct gccatatcat cgtggcgagg gagaccggag
    47521 agattatcga caggagtgta gagccagctg gcttcgctct ctgcgatata gacaggagga
    47581 ttgccaaacg tctcgcgaac caagtccaga ctcatgatat ggtcataatg ggcgtgggtc
    47641 aagagaatag cacagatcgg tttgttgatc ttctcgattg tctgacgaat ggcttcccaa
    47701 tgactaccag gatcgacgac gatgaggtgc ttttcgcctt ctagataata ggtattttca
    47761 taggcaacag gattcacggt tttatggatt ttcatactag ctccaatctc aaagaatgta
    47821 ctaatactct tcgaaaatct cttcaaaccg cgtcaacttt atctgcaacc tcaaagcagt
    47881 gctttgaaca gcctgcggct agctttctag tttgctcttt gattttcatt gagtataaat
    47941 taagtatagc acagttaggg agaataggta aggatttaaa atgaacttaa aaaccagcat
    48001 gactagatga agacaggctg gttatcaatt tatcaatatt ggaaaggtgt caattgccct
    48061 tcataggttg cataaactcc gtcattagct tgtttgatag acgtgatgtt tagagtgcta
    48121 ccgtagttgg cttctcattt gtcgctatat tcactataag ttatcctact gggcaggttc
    48181 tggtcgttag catagtattg aacgagtttt ttatagcttt gctggctgaa taggaacagg
    48241 cagcaaactc ctaacattag gagagcaaag atagaaatag cagttttttt cataggtatt
    48301 ctccttataa actaaaggat agaagaaagt atcttgagga tttttatact cttcaaaaat
    48361 ctcttcaaac cgcgtcaacg tcgccttgcc gtatatatgt gactgacttt gtcagtctta
    48421 tctacaacct caaagcagtg ctttgagcaa cctgcggcta gtttcctagt ttgctcttgg
    48481 attttcattg agtattaatt ccaaaatcca gtcaaggtac cagtgtatgt gatacgagtt
    48541 ccaccgttta tctttcgtat cttcaatgga attggggaaa tcagaaagtc tttgggttac
    48601 aatatggttt ccgttcttct atctctcctt ctatcaccta agtttaaaat ctaacttcgt
    48661 agtatattat gggccaataa ttcagaatta tcaagcaatt ttgaattatt tttttcttat
    48721 tctgttccaa tctagttttc atattcttaa aaaagtgata aaatggtagg aagaattgga
    48781 gagtagagat gccgaaagaa gtgaatttaa caggcgaaga agttgtcgct ttaaccaaag
    48841 aatatttaac ggaagaggat gttcattttg tccataaggc cttggtctat gctgttgaat
    48901 gccacagtgg tcaatatcgc aaatcaggcg agccttatat cattcaccct atccaagtgg
    48961 caggtatttt agctaagcta aagctggatg ctgtaacagt agcttgtgga ttcttgcatg
    49021 atgtggtgga agatacagat gcgaccttgg acgatttgga aagagagttt ggtcctgatg
    49081 tgcgggtgat tgttgacgga gttaccaagc ttggcaaggt cgagtacaaa tcaatcgagg
    49141 agcaattagc ggaaaatcat cgcaagatgc tcatggccat gtctgaggac atccgcgtta
    49201 ttttggtcaa actgtctgac cgcttgcaca atatgcggac cctgaaacat cttcgaaaag
    49261 acaagcagga gcgtatttcc aaagaaacca tggaaatcta tgccccactt gcccatcgtt
    49321 tggggatttc cagtgtcaaa tgggaattag aagacttgtc tttccgttat ctcaatccaa
    49381 cggagtttta caagattacc catatgatga aggaaaagcg cagggagcgt gaggccttgg
    49441 tggatgaggt agtcacaaaa ttagaggagt atacgacaga acgtcacttg aaagggaaga
    49501 tttatggtcg tcccaagcat atttactcaa ttttccgcaa aatgcaggac aagagaaaac
    49561 ggtttgagga aatctatgat ctgattgcta ttcgttgtat tttagatacc caaagtgatg
    49621 tttatgccat gcttggttac gtgcatgaat tttggaaacc gatgccaggt cgcttcaaag
    49681 actatattgc caaccgcaag gccaatggtt atcagtctat ccatacgact gtttatgggc
    49741 caaaagggcc gattgaattc cagattcgaa ccaaggaaat gcacgaggtg gctgagtacg
    49801 gggttgcggc tcactgggct tataagaaag gtataaaggg gcaagttaac agcaaggaat
    49861 cagctattgg aatgaactgg atcaaggaga tgatggagct ccaagaccag gctgatgatg
    49921 ctaaggaatt tgtggactct gttaaggaaa actatctggc tgaggagatt tacgttttta
    49981 ccccagatgg agctgtccgt tcccttccca aagattcagg accgattgat tttgcctacg
    50041 aaatccatac caaggtcggt gaaaaagcaa ctggtgccaa ggtcaatggc cgcatggttc
    50101 cactgacaac caagttaaag acaggggatc aggttgaaat tatcgccaac ccgaactcct
    50161 ttggacctag ccgtgactgg ctcaatatgg tcaagactag caaggcgcgc aataagattc
    50221 gccagttctt taaaaaccaa gataaggaat tgtctgtcaa caagggtcgt gagatgctga
    50281 tggctcagtt ccaagaaaat ggctatgtgg caaataaatt tatggacaag cgccacatgg
    50341 atcaagttct gcaaaagacc agttacaaga cagaagactc cctctttgcg gccattggtt
    50401 ttggggaaat cggtgcgatt accgtcttta accgtctgac tgaaaaggaa cgccgtgagg
    50461 aagagcgtgc caaggccaag gctgaggcag aggagcttgt caaaggtggc gaggtcaagg
    50521 ttgaaaataa agaaactctc aaggtcaagc atgagggggg agtggttatt gaaggtgctt
    50581 ctggtctcct agtgcggatt gctaagtgtt gtaaccccgt gcctggtgac gatattgttg
    50641 gctacattac caagggtcgt ggtgtggcta ttcaccgtgt ggactgtatg aacctgcgtg
    50701 cccaagaaaa ctacgagcaa cgtctccttg atgtggaatg ggaagaccag tactctagct
    50761 caaataagga gtatatggcc catatcgata tctacggtct caaccgtaca ggactgttga
    50821 acgatgtact gcaagttctt tcaaatacaa ccaagaatat ttcaacggtc aatgcccaac
    50881 caaccaagga tatgaagttt gctaatatcc atgtgtcctt cggtattgcc aacctctcta
    50941 cactgaccac ggttgtcgat aaaattaaga gtgtgccaga agtttactct gtcaaacgga
    51001 ccaacggcta gttgtcctag ctcttactag aaaggctatt atgaaaatca ttatccaacg
    51061 ggttaaaaaa gcccaagtga gtatagaagg ccagattcag ggaaaaatca atcagggact
    51121 tttattgcta gttggtgttg gaccagagga ccaagaggaa gatttggact atgctgtgag
    51181 aaaactggtc aatatgcgga ttttttcaga cgcagaaggc aagatgaacc tgtctgtcaa
    51241 agatattgaa ggagaaatcc tctctatttc tcagtttacc ctctttgcgg atactaagag
    51301 aggcaatcgt ccagccttta caggggcagc taaacctgat atggcatcag acttctatga
    51361 tgctttcaat caaaaattag cgcaagaagt gcccgttcag acaggtatct ttggagcaga
    51421 tatgcaggtt gagctggtta ataacggacc tgttaccatt atcctagata ctaaaaagag
    51481 ataagaaaga ccaagcccag tcggcttggt ctttctcatc gatcataaaa tactccaaaa
    51541 agaaatcggt tcttgatatg cttgggggaa gtctcttttc aggctttggc agatgcgata
    51601 ggaagggatg agatgtccta ggatgaggag agttccctga aggaaaagtg gaatatcact
    51661 taaagacacc aaggagagaa tcatcaggct atcccatagg agaatttgta aagcaaaacg
    51721 ccagaatctc ggtctaatct gggaatagag ttgactaaag tggtcacaaa gttggtttga
    51781 aagataggtc aatagcacag gatgaaagaa aatgagccaa ccgctataaa tcaaaatcca
    51841 gtcccaagta agcccccctt tttaaatgtc aaaaatgcca gcatgattcc atcaatgaca
    51901 aggagttgaa tggttttgat gaagatgatt ttttcatgct aaaacctcct tttccttaga
    51961 gacactccca taaagagatg tgtttgtcgt aaaagtctgg atagttttct cttaggtagt
    52021 cagctgtcat ataataaaaa gtagaatggc aagcagtgat aattgacata agagatgaaa
    52081 aacgctgaga actaatgaag gctaagagaa caaaaaatag aaaatcaatg gtgagaaccc
    52141 ctaaatagta gaagcgaatc tttttattga tttgaggata aatctcagcg aattgatgtt
    52201 tgagtcggac gatcaagaga aatagcagga gtccttgagc aaggaagaaa attaaaccag
    52261 agagcaagag gcactcaaaa gatgtctcga gtctagtgat aaaaaatgca aatagtagca
    52321 aatcaataac tgcaattgct ataaaatgga ttctatagag ttttttcatg acaagacctt
    52381 cctcttttat ctaacttcat tctactccaa aagaatggga gttacaacta aaatgataaa
    52441 aatagcagaa gggagattct cttaagttgg ctagtattct ttatttgagt ttccttctat
    52501 gtatctaact tcttcatcat tccagacaaa taaagctccg attgcattga ggatataaaa
    52561 gatgtattta ccgatattgg cgaagtttcc ttgaatacca gcttttgtca gctgaacgaa
    52621 attgtaaatc aaccaaaagc cccactgagt tgttagtttc aatgcattca aagcattggc
    52681 aatgagggac agtgcaaagg caatagttgt tacgtaggca aggagatcca tcttgccctc
    52741 atatccgata tagttggtca caaaggcaaa gaggaaggcg atgatggaaa tgatgatggc
    52801 cgccaatttt acctgttttt ggctcatttg gttgggtctg ccttcttgcg aagcttccca
    52861 cttctttata gcaaaggtat aaatgaggaa ggtgacggga taggtaatga tggccgcctt
    52921 atttccaagg atataatcaa tagcaccgaa caaaagggta ttaacaatac caaagtaatt
    52981 tccccatttg cttaatttcc ccgtgaaacg agtggacaac atggaaatcc caacgttggt
    53041 tacggaaatc aatccaaagg gtacaagagc tgtccatgat ccccagtcta caaatttatc
    53101 gaggcgtgag ttgaggtaac cagatgcaat cgcaatccca acgaccaaag caaccccgaa
    53161 gaggtcaaac catttagatg tagcaaaaat ttttagtgat tttttcatag gttaaactac
    53221 ctttcttttt tttttcaaat attctaccac caaatgaaag taaaataaaa tgatagaaat
    53281 aaaaccctga aaataaaact ttcagggttg gtaggggact tgagaaatta gttgattttc
    53341 tcgacataga gttgttttgc aatgtccata ttcacttgta aatcctcgtc gttactgagg
    53401 atagtgaagg tattgctgaa gccatcaaac tgcttaactt ggagctggtc accgatgtga
    53461 agtgagtgct tgtccagata atggagaatg tcaaaactat cgtgcacccg agtcaggcgg
    53521 taggcgccag cttccttgat atcagctagt gggaggttat tgatttcaac gaggagttct
    53581 cccttggcag gaatagttcc tccgtggggg caggttttag ggaaacctag cagtttatct
    53641 agtctttcca cgaacaggtc agagacagtg tgttccaaga cctcagcttc ctcgtgaatc
    53701 tggtcacttg tatagtctaa atgatgaact agaaaaactt caatcaagcg gtgcttacga
    53761 tagagctcag agaccagttt gagaccgagg tcagtcagta gatagccaca ttccttgtcc
    53821 tttaggatga gattttcact tttcattcgt ttgatcattt cagttacggc agggggagag
    53881 acttgcatgc gagccgcaat ttccttgttg gtaatcttat gcaggtctat gccaatttca
    53941 taaatacatt ttagatagtc ttctttgttt ggggtcattc gtttcctctt gtctaatatc
    54001 tttatctagt atatcaaaaa aagctagatt tctctagctg tgacttttta tgttatactt
    54061 attgcaagag aaaaaacgag gagatggata tgacgaaaat agctcttctt tcagatattc
    54121 atggaaatac caccgccttg gaggctgttt tggcagatgc tcggcagata ggagtggatg
    54181 aatactggct tttgggagat attctcatgc cagggacagg gcgtagaagg attttggact
    54241 tgttggatca actaccaatt acggctagag ttttgggaaa ctgggaagat agtctttggc
    54301 atggtgtccg taaggaattg gacagtactc gccccagtca acgctatctc ttgcgccagt
    54361 gccagtatgt tttagaggaa atttccctag aagaaattga agtgctccac aatcaacctc
    54421 ttcaaattca tcgtcagttt ggggatttga cggtgggaat tagccatcat ctgcctgata
    54481 agaactgggg gcgagagttg attcatactg gcaaacaaga ggagtttgac cgcttggtga
    54541 ctcatcctcc ctgtgatatt gctgtttatg gacatattca ccagcagttg cttcgttacg
    54601 ggactggtgg gcaattgatt gtcaatccgg gttcgattgg ccaacctttc tttttggacg
    54661 ctcagctacg gaaggacttg cgggcccagt atatgatttt ggagtttgat gacaaaggcc
    54721 tggtagatat ggacttccga cgggtagact acgatgtagc agctgaattg cagctggcta
    54781 aagacctgag acttccctat tttgaggttt actatgaaag tctggtcaat gggatccacc
    54841 atactcatca tcaggaattt ttgagagaat tggctcagaa agagggctgc gatcgggagt
    54901 tagacgactg gttgaaaagt ggtaacgatt gacattacaa ctaaaaaggg tataataata
    54961 gaaaaagaag agtagaggca gggtagcctc gtaaaaagga gaagaggatg caaattccaa
    55021 gtagatttac cattgcgact catatgctga taatcattgc cctcgagggg aaggaaagca
    55081 aggtgaccag tgattttctg gctgctagtg tcggggtcaa tcctgtcatt atcagaaaga
    55141 tcttgtccca gttgaagaag gcagagctga tttcagtagc gcgtggaacg ggcggaacag
    55201 agattgtcaa ggaccttaag gatattagtc ttttagatgt ttatcaggcg gtcgaatgtc
    55261 ttggtaagac aggtcaactc ttcagtttcc atgacaatcc gaatccaaat tgccctgtag
    55321 gagctcatat tcatgatgtt ttggatcaaa aattggagag aattcagttg actatggagg
    55381 cagaacttgg tcaaaccagt ctagaaaaag tcgtggccga tgcagagagt cagatgaagg
    55441 attaagaggg ataaatgccc tcttttttct ttggataatt atgataaaat gtagctatta
    55501 aaggagtaag aaatgtatct cgttatagga attgtattag cctttatagt gtcattttgg
    55561 aaggataata gaagtttgtg gaatccagta ttatttttat tatccctcat ttcaagttat
    55621 ttttatctat cctacctttt ttataaaaat ggatacgaga atgttcagtt agctttttat
    55681 gtatttgcct ttgtcttact tccttttttg ctttttctaa gtggcatctt tttaatctat
    55741 aatggggtca ttttgttgaa gcgagaggga cgttccaagc cccattatct ctcaatgctc
    55801 tttgattttt attgactatt agattctatt taaaaagact tagaatgcac atgaaagtat
    55861 gtattctagg tcttttgttt tgtatacatt taattttcaa gagtattatc tatttttcca
    55921 tgcctggtaa cgagtatcaa tcaataactg ggtaaggcga cccaaggttt ctctttcttc
    55981 tggagtgagg gattgagctt ggacaaagag atgatgaatg tattcaagcg cctttaagga
    56041 agccaagtca ttgatagagc taataccagc ctcaagaata gtgccaaaga agaggtcgaa
    56101 gtagagctga agttcttcgt cagggactgt ggccacccat tctttaaagg ttgtgtctac
    56161 ttgctggcta tcactgttgg tcttatccag ttggacgaag tgcttgtcct caatctgcca
    56221 actaaaggta tcgtgctggg cgatgccacc cagggcagta ctctgaacga tgatttggtg
    56281 agcaggaatt tccagcatca taccgataat ggaaccttgt ggaatgaata tcttgcttct
    56341 atccattatc ctttgataac ccgcagtctg tgtcaattct tgatggagac caggtgcatc
    56401 aaatgtatag actgctgtga tctgattttg caaactttgc tcaatttggc tagcagcata
    56461 gatagcgaga tttcctccct tggaatgccc agccagaata accttttgct taggatgatg
    56521 ggcaaaaaag ttctttaaat agcgaagggc gtgcttttga gcaggaattt ccttcatata
    56581 ggtcaggtgg aaatcttcct tccagccaat gatactgtca tctgtcccac gaaagacaat
    56641 cagataggta tcgaggctga cacgataagt catagccgca aattgctttt gcagttcagg
    56701 gtcgatgtcg ttgataaaat gggagagttt gcaatttttg aagcgcttgt gttgagccaa
    56761 ttcatctaat aattgaaggc gatttttgct agtaagcatg gtgggctctc ttggaacctg
    56821 aggtgctaga tctaaaagac gttgaggaag tgtggagacc agattatcaa aggagaggta
    56881 ggtgatttct attaaggtta gaatgtctaa ctcattcaag ggaaggtcgt aataagaatc
    56941 atatgcgaca tctttcagat agtcaaaaat attggccata agagctcctt tctttttatt
    57001 tatcttagca aatttccctc aaattagcta gtagcatagc ctgtttgtac tggctaaaaa
    57061 caggctattt caaattcagt ttcagaccat ctagcatgga aaaatctgtt ataataatgg
    57121 aaaaggagaa gcgcatgcac aagattttat taatagaaga tgatcaggtc attcgtcaac
    57181 agattgggaa aatgctctct gaatggggat ttgaagttgt cctggtagaa gactttatgg
    57241 aagttttgag tctatttgtt cagtcggaac ctcatctggt cctcatggat attggtttgc
    57301 ccttgtttaa tggttatcac tggtgtcagg aaatccgcaa gatttccaag gtacctatca
    57361 tgtttctttc ttcgagagac caggctatgg atattgtcat ggcaatcaat atgggggcgg
    57421 atgactttgt gaccaagcct tttgaccagc aggttctttt agctaaggtt cagggcttgt
    57481 tgcgtcgttc ctatgagttt gggcgtgatg agagtttgct ggaatatgct ggtgttatcc
    57541 tcaataccaa atccatggat ttacattatc aagggcaagt cttgaatttg accaagaatg
    57601 aattccagat tttacgcgtg ttatttgagc atgcaggcaa catcgtagca cgtgacgacc
    57661 tgatgcggga actttggaac agtgactttt tcattgatga taataccctc tctgtcaatg
    57721 tggctcgttt gcgtaaaaag ttggaggagc agggattggt aggatttatc gagaccaaga
    57781 aaggaatagg gtacggattg aagcatgctt gattggaaac aattttttct agcctatctg
    57841 cgctcccgtg gtcgtctttt tatctatctg ctttctttgg catttcttgt cttactcttt
    57901 cagtttttat ttgccagtct aggaatttac ttcctctact ttttcttctt gtgttgcttt
    57961 gtaaccatct tatttttcac ttgggacata ttggtggaaa cgcaggtcta tcgccaggaa
    58021 cttctctatg gagagaggga agccaagtct cctttggaaa tagctttagc agaaaaatta
    58081 gaagcgcgtg agatggaact ctatcagcag aggtcaaaag cagaaagaaa actgacggat
    58141 ttgctggatt actatacctt gtgggtccat cagataaaga cccccattgc agccagtcaa
    58201 ctcttagttg cagaagtggt cgaccgccaa ctgaagcagc agctagaaca ggaaattttc
    58261 aaaatcgact cctataccaa cctagtttta cagtacctgc gtttagaaag tttccatgat
    58321 gatttgctct taaagcaggt tcaaattgag gacttggtca aggaaataat tcgtaaatat
    58381 gctcttttct ttattcaaaa aggcttaaat gtcaatctac atgaccttga taaagaaatc
    58441 gtgacggata aaaagtggct gctagtggtt attgagcaaa tcatctcaaa cagtctcaag
    58501 tacaccaagg aaggtggtct ggagatttat atggatgacc aagagctttg tatcaaagat
    58561 acgggaatcg ggataaaaaa cagtgatgtc ctccgagtat ttgaacgtgg cttttcagga
    58621 tacaatggcc gtttgaccca gcagtcctct ggacttggcc tttatctatc taagaaaatt
    58681 tctgaagaac tggggcacca gattcgtatc gagtctgagg tcggaaaagg aacgacagtg
    58741 cggattcagt ttgctcaagt gaacttagtc cttgagtaaa aatagatgag aaaaagcctc
    58801 cagattggag gcttttatgc tagactctta tttctcaacg cgtgatttgt tggcgatatc
    58861 agctaggata gcttgaacaa aattatcaac tgagacagtt tgtgtttctt tttggccgta
    58921 gcgacgaacg ttgactgttt cgtcttccat ttctttgtct ccaacaatta attggtaagg
    58981 aatcttgctg gtttgtgaag cacggatctt gaactgcatt ttttcattgc gctcatctac
    59041 gtctgcacgg acaccgcggt cacggagttt cttggccact tcccaagcgt agtccacgtg
    59101 tttttcgtta gatactggga tgagggttac ttggtgtggt gccagccatg ttgggaaggc
    59161 ccccttgtag ttctcaatca agatagctgt gaagcgttcc atagttgaga taaccccacg
    59221 gtggatcatg actggacggt gatcttcgcc atcagctccg atgtatttga ggtcgaagcg
    59281 ttctggcaac aagaaatcaa gttggatagt agaaagggtt tcttcttttc caagggcagt
    59341 tttaatctgg atatccaatt ttggtccgta gaaggctgct tcaccttcgg cttcaaagta
    59401 gtccacgccc atttcatcaa gagctgcacg aagcatggtt tgggcatttt cccacatctc
    59461 atcgttatca aagtacttat gagtatcttg agggtcacga agagagaggc ggaagcggta
    59521 gtcagtcaag ttgaagtctt cataaacatc gataatcaac tgaagggcac gttggaattc
    59581 ttcttggatt tgttctggag taacgaatag gtgaccgtcg ttgagtgaca tttcacgtac
    59641 acgttgaagg ccagtgaggg caccagattt ttcgtaacgg tgcatcatac cgatttcagc
    59701 gatacggatt ggcaattcac ggtaagagtg aacatggtgt ttgaaaactt ggatgtggtg
    59761 cggacagttc attggacgaa ggacaaattc ttccccgtca cccatgtcca tggttgggaa
    59821 catgtcttct tggtaatgat cccagtgacc agaagtcttg taaagctcaa cagaagcaag
    59881 tggtggagtg tagacgtgtt ggtagccaga agccaactct ttgtttacga tgtagcgttc
    59941 caattcacga cggatagtcg caccatttgg caaccagaat ggcaaacctt gtcccacttc
    60001 ttgtgaaatc ataaagaggt caagctcttt accaagttta cggtggtcac gttccttagc
    60061 ttcttcacgc atttgaaggt agtttttcaa gtctttcttg tcaaaccaag ctgtaccgta
    60121 gatacgttgc atcatagcgt tgtcgctgtt tccacgccag tacgcaccag ctacatggag
    60181 aaggtggaag atttggatac gacctgttga tggaacgtga ggtccacggc agaggtctac
    60241 atattcaccc tgacgataga tagtcaaacc gccttcgtct tctgagtgtt cttcaatcaa
    60301 ttccaacttg taagggtcat ttttgaagat ttcacgtgcc tcgtctttag tcacttcttc
    60361 acgaatagat gggaagtttt ctttgacgat tttttgcatt tcttcttcga tacgaggaag
    60421 gtcttcgtta gagatttgac cagctgtgtt gtcagtatcg tagtagaaac catcttcgat
    60481 ggctggacca actcccaagt gaatgtctgg gaaaagacga cgagctgctt gggcgaacaa
    60541 gtgagctgct gagtgacgca agattggaag ggcatcttcg tgatcaggtg tcacaatttc
    60601 gatgcttcca tcttcagtga tagcgcgagt agtgtcgatg agtttgccgt tgaatttacc
    60661 agccaaggct tttttagcta gggaattgct gatagattgg gcaatttcaa aagttgttac
    60721 gccagattcg aattcacgaa cagcgccatc tgggaaagta atgttaatca tgatgttctc
    60781 cttttttatt atttcatagt ttctaagcat tgatttttta tcagcagact acttccgagt
    60841 cctaacgttc aattgctttt ctttatcaga gaggaagttt tccatttccg tcagaatgtc
    60901 tatatccaaa cgttgagaaa gttctaatag ccaccagata ttttctgaaa gtttttgttc
    60961 cagtgtgtag ggtgtttcat catagtagcg tccttgcttt gtcatcacca gtcattggaa
    61021 atttccaata tcattagata aagccaagag gtcttcttct accgtccact tgaaatcatg
    61081 atgcttaact tccagttcgt gataagcttg tcggattgcc caactgcgct ccactaattc
    61141 ttgcaactcc ataattagta tcctaataaa aaaatgcgac atcctcgaaa ggacgtcgca
    61201 acgtggttcc accttcattt atgtacctca aaagagaggg acacctctga tcggctctaa
    61261 cgtggccacc gttttatgtt ttcataaaaa actcaagagt agtatcagtt tagacttcct
    61321 atgcgtttcc agcaaccacg cactctctag tagaaaggga ctaagtgact tgtctctatg
    61381 gattattata gcatgattgt gagatttttc aaggattttg tgaaagtttt ttgtttttca
    61441 ttttttgtag aatatgcttg atgctaatac cagaatccat gccgaccaga gcaactgttt
    61501 catagattag caagtaaatt agtataaact caccaaaggt tatgatagta aagtaaaaga
    61561 gcagtccgtt accaactaac catcaaagtg agaaacggaa acggtagcta tagagtaacg
    61621 atataatcaa gattaccaga ggtgtaaaga agagaagatt gtttatatag agttccttga
    61681 gtgtaagggg gtcagaaaca actagtaaga acaactcata aagtggccaa taaaagaata
    61741 atatgaccag atagtagggg agatgggaat agaatctacg catgataatc acctcgtttt
    61801 aaaaaaacag acaacttaga tctcttgcta tgattattat agcatgttta aaacaagaaa
    61861 gtaaatttgt tgacaaagaa gatttttctt ttcactagaa tcatagttag ttttcttg
//
LOCUS       DAVBGY010000005        59122 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVBGY010000005 DAVBGY010000000
VERSION     DAVBGY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 59122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59122
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.005"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(133..1941)
                     /gene="glmS"
                     /locus_tag="VQ620_000292"
     CDS             complement(133..1941)
                     /gene="glmS"
                     /locus_tag="VQ620_000292"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET2762982.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQVFDLVHELSIVAQSIESTLSEKET
                     IEVKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(2133..3512)
                     /gene="bglA"
                     /locus_tag="VQ620_000293"
     CDS             complement(2133..3512)
                     /gene="bglA"
                     /locus_tag="VQ620_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET2762983.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KKLSDNNGF"
     gene            complement(3611..5464)
                     /locus_tag="VQ620_000294"
     CDS             complement(3611..5464)
                     /locus_tag="VQ620_000294"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET2762984.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(5477..6736)
                     /gene="rseP"
                     /locus_tag="VQ620_000295"
     CDS             complement(5477..6736)
                     /gene="rseP"
                     /locus_tag="VQ620_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET2762985.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(6758..7561)
                     /locus_tag="VQ620_000296"
     CDS             complement(6758..7561)
                     /locus_tag="VQ620_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET2762986.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(7570..8328)
                     /locus_tag="VQ620_000297"
     CDS             complement(7570..8328)
                     /locus_tag="VQ620_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET2762987.1"
                     /translation="MFGLFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(8559..9131)
                     /locus_tag="VQ620_000298"
     CDS             complement(8559..9131)
                     /locus_tag="VQ620_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET2762988.1"
                     /translation="MSTMKNKQEILEAFRKNPDMMAILTIIQNLELTDSWLAAGSVRN
                     FIWNLLSDKSPFDFETDVDVIFFDPDISYEETLLLEKKLREDFPQYHWELKNQVYMHQ
                     HSPHTAFYTSSRDAMSKYPERCTAVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(9112..10083)
                     /gene="ruvB"
                     /locus_tag="VQ620_000299"
     CDS             complement(9112..10083)
                     /gene="ruvB"
                     /locus_tag="VQ620_000299"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET2762989.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDVITDKALTMLDVDHEGLDYVDQKILRTMIEMYGGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRMATAKAYEHLGYEYNEK"
     gene            10244..10336
                     /locus_tag="VQ620_000300"
     CDS             10244..10336
                     /locus_tag="VQ620_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HET2762990.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            complement(11151..11567)
                     /locus_tag="VQ620_000301"
     CDS             complement(11151..11567)
                     /locus_tag="VQ620_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2762991.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(11579..12274)
                     /locus_tag="VQ620_000302"
     CDS             complement(11579..12274)
                     /locus_tag="VQ620_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2762992.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            complement(12468..14969)
                     /locus_tag="VQ620_000303"
     CDS             complement(12468..14969)
                     /locus_tag="VQ620_000303"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET2762993.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGDVKEAAYTEDGLHVNSDFLDELNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            complement(15424..16512)
                     /locus_tag="VQ620_000304"
     CDS             complement(15424..16512)
                     /locus_tag="VQ620_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HET2762994.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(16530..17198)
                     /locus_tag="VQ620_000305"
     CDS             complement(16530..17198)
                     /locus_tag="VQ620_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HET2762995.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            complement(17217..19655)
                     /locus_tag="VQ620_000306"
     CDS             complement(17217..19655)
                     /locus_tag="VQ620_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HET2762996.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(19806..21104)
                     /locus_tag="VQ620_000307"
     CDS             complement(19806..21104)
                     /locus_tag="VQ620_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2762997.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSMTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFVAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLAMSTMVYFPFFKIQDRLAYQNEIKQS"
     gene            complement(21121..21429)
                     /locus_tag="VQ620_000308"
     CDS             complement(21121..21429)
                     /locus_tag="VQ620_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2762998.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            complement(21475..21795)
                     /locus_tag="VQ620_000309"
     CDS             complement(21475..21795)
                     /locus_tag="VQ620_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2762999.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            complement(21990..22970)
                     /locus_tag="VQ620_000310"
     CDS             complement(21990..22970)
                     /locus_tag="VQ620_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HET2763000.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            complement(22983..23729)
                     /locus_tag="VQ620_000311"
     CDS             complement(22983..23729)
                     /locus_tag="VQ620_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2763001.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            23851..24627
                     /locus_tag="VQ620_000312"
     CDS             23851..24627
                     /locus_tag="VQ620_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HET2763002.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            24702..24899
                     /locus_tag="VQ620_000313"
     CDS             24702..24899
                     /locus_tag="VQ620_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET2763003.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            25116..25712
                     /locus_tag="VQ620_000314"
                     /pseudo
     CDS             25116..25712
                     /locus_tag="VQ620_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            25869..26237
                     /locus_tag="VQ620_000315"
     CDS             25869..26237
                     /locus_tag="VQ620_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2763004.1"
                     /translation="MAVGLSYEDPAVKLLNDGANIKVVYPKEGTVFLPASAAIVKKAK
                     NMENAKKFIDFIISQEVQDTLGTTTTNRPVRKNAKTSENMKPIDKIKTLTEDYDYVIK
                     NKSDIVKKYNEVFTDIQSKQ"
     gene            26250..26636
                     /locus_tag="VQ620_000316"
     CDS             26250..26636
                     /locus_tag="VQ620_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2763005.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGLVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYAD"
     gene            26643..26732
                     /locus_tag="VQ620_000317"
                     /pseudo
     CDS             26643..26732
                     /locus_tag="VQ620_000317"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            26718..27341
                     /locus_tag="VQ620_000318"
     CDS             26718..27341
                     /locus_tag="VQ620_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2763006.1"
                     /translation="MDEPLSNLEAKLRLDMRQAIRAIQHEVGITTVYVTHDQEEAMAI
                     SDQIAVMKDGVIQQIGRPKELYHKPANEFVATFIGRTNIIPTNLEKRSDGAYIVFSDG
                     YALRMPALDQVEEQAIHVSIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASK
                     IQVSEESTFEEELQKGNRIRLRINTQKLNIFSADGSQNLIKGVNHGM"
     gene            27331..28983
                     /locus_tag="VQ620_000319"
                     /pseudo
     CDS             27331..28983
                     /locus_tag="VQ620_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(29086..29778)
                     /locus_tag="VQ620_000320"
     CDS             complement(29086..29778)
                     /locus_tag="VQ620_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2763007.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(30022..31359)
                     /locus_tag="VQ620_000321"
     CDS             complement(30022..31359)
                     /locus_tag="VQ620_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HET2763008.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMRVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(31369..31635)
                     /locus_tag="VQ620_000322"
     CDS             complement(31369..31635)
                     /locus_tag="VQ620_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET2763009.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAIVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(31900..32286)
                     /gene="rplQ"
                     /locus_tag="VQ620_000323"
     CDS             complement(31900..32286)
                     /gene="rplQ"
                     /locus_tag="VQ620_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET2763010.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(32298..33233)
                     /locus_tag="VQ620_000324"
     CDS             complement(32298..33233)
                     /locus_tag="VQ620_000324"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET2763011.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(33276..33659)
                     /gene="rpsK"
                     /locus_tag="VQ620_000325"
     CDS             complement(33276..33659)
                     /gene="rpsK"
                     /locus_tag="VQ620_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET2763012.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(33677..34042)
                     /gene="rpsM"
                     /locus_tag="VQ620_000326"
     CDS             complement(33677..34042)
                     /gene="rpsM"
                     /locus_tag="VQ620_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET2763013.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(34060..34176)
                     /gene="rpmJ"
                     /locus_tag="VQ620_000327"
     CDS             complement(34060..34176)
                     /gene="rpmJ"
                     /locus_tag="VQ620_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET2763014.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(34201..34419)
                     /gene="infA"
                     /locus_tag="VQ620_000328"
     CDS             complement(34201..34419)
                     /gene="infA"
                     /locus_tag="VQ620_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET2763015.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(34536..35174)
                     /locus_tag="VQ620_000329"
     CDS             complement(34536..35174)
                     /locus_tag="VQ620_000329"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET2763016.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            complement(35325..36635)
                     /gene="secY"
                     /locus_tag="VQ620_000330"
     CDS             complement(35325..36635)
                     /gene="secY"
                     /locus_tag="VQ620_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET2763017.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(36648..37088)
                     /gene="rplO"
                     /locus_tag="VQ620_000331"
     CDS             complement(36648..37088)
                     /gene="rplO"
                     /locus_tag="VQ620_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET2763018.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(37233..37457)
                     /gene="rpmD"
                     /locus_tag="VQ620_000332"
     CDS             complement(37233..37457)
                     /gene="rpmD"
                     /locus_tag="VQ620_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET2763019.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(37429..37923)
                     /gene="rpsE"
                     /locus_tag="VQ620_000333"
     CDS             complement(37429..37923)
                     /gene="rpsE"
                     /locus_tag="VQ620_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET2763020.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(37941..38297)
                     /gene="rplR"
                     /locus_tag="VQ620_000334"
     CDS             complement(37941..38297)
                     /gene="rplR"
                     /locus_tag="VQ620_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET2763021.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(38381..38917)
                     /gene="rplF"
                     /locus_tag="VQ620_000335"
     CDS             complement(38381..38917)
                     /gene="rplF"
                     /locus_tag="VQ620_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET2763022.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(39109..39507)
                     /gene="rpsH"
                     /locus_tag="VQ620_000336"
     CDS             complement(39109..39507)
                     /gene="rpsH"
                     /locus_tag="VQ620_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET2763023.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(39792..40061)
                     /gene="rpsN"
                     /locus_tag="VQ620_000337"
     CDS             complement(39792..40061)
                     /gene="rpsN"
                     /locus_tag="VQ620_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HET2763024.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(40079..40621)
                     /gene="rplE"
                     /locus_tag="VQ620_000338"
     CDS             complement(40079..40621)
                     /gene="rplE"
                     /locus_tag="VQ620_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET2763025.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(40645..40950)
                     /gene="rplX"
                     /locus_tag="VQ620_000339"
     CDS             complement(40645..40950)
                     /gene="rplX"
                     /locus_tag="VQ620_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET2763026.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(41028..41396)
                     /gene="rplN"
                     /locus_tag="VQ620_000340"
     CDS             complement(41028..41396)
                     /gene="rplN"
                     /locus_tag="VQ620_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET2763027.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(41422..41682)
                     /gene="rpsQ"
                     /locus_tag="VQ620_000341"
     CDS             complement(41422..41682)
                     /gene="rpsQ"
                     /locus_tag="VQ620_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET2763028.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(41707..41913)
                     /locus_tag="VQ620_000342"
     CDS             complement(41707..41913)
                     /locus_tag="VQ620_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET2763029.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(41923..42336)
                     /gene="rplP"
                     /locus_tag="VQ620_000343"
     CDS             complement(41923..42336)
                     /gene="rplP"
                     /locus_tag="VQ620_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET2763030.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(42340..42993)
                     /gene="rpsC"
                     /locus_tag="VQ620_000344"
     CDS             complement(42340..42993)
                     /gene="rpsC"
                     /locus_tag="VQ620_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET2763031.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(43006..43350)
                     /gene="rplV"
                     /locus_tag="VQ620_000345"
     CDS             complement(43006..43350)
                     /gene="rplV"
                     /locus_tag="VQ620_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET2763032.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(43362..43643)
                     /gene="rpsS"
                     /locus_tag="VQ620_000346"
     CDS             complement(43362..43643)
                     /gene="rpsS"
                     /locus_tag="VQ620_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET2763033.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(43747..44580)
                     /gene="rplB"
                     /locus_tag="VQ620_000347"
     CDS             complement(43747..44580)
                     /gene="rplB"
                     /locus_tag="VQ620_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET2763034.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(44598..44894)
                     /locus_tag="VQ620_000348"
     CDS             complement(44598..44894)
                     /locus_tag="VQ620_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET2763035.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(44894..45517)
                     /gene="rplD"
                     /locus_tag="VQ620_000349"
     CDS             complement(44894..45517)
                     /gene="rplD"
                     /locus_tag="VQ620_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET2763036.1"
                     /translation="MANVTLFDQTGKEAGQVVLNDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(45542..46168)
                     /gene="rplC"
                     /locus_tag="VQ620_000350"
     CDS             complement(45542..46168)
                     /gene="rplC"
                     /locus_tag="VQ620_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET2763037.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(46385..46693)
                     /gene="rpsJ"
                     /locus_tag="VQ620_000351"
     CDS             complement(46385..46693)
                     /gene="rpsJ"
                     /locus_tag="VQ620_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET2763038.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(46959..47576)
                     /locus_tag="VQ620_000352"
     CDS             complement(46959..47576)
                     /locus_tag="VQ620_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HET2763039.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(47573..48163)
                     /gene="nrdG"
                     /locus_tag="VQ620_000353"
     CDS             complement(47573..48163)
                     /gene="nrdG"
                     /locus_tag="VQ620_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HET2763040.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            complement(48156..48662)
                     /locus_tag="VQ620_000354"
     CDS             complement(48156..48662)
                     /locus_tag="VQ620_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2763041.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            complement(48706..48846)
                     /locus_tag="VQ620_000355"
     CDS             complement(48706..48846)
                     /locus_tag="VQ620_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763042.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(48858..51065)
                     /gene="nrdD"
                     /locus_tag="VQ620_000356"
     CDS             complement(48858..51065)
                     /gene="nrdD"
                     /locus_tag="VQ620_000356"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HET2763043.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKQEDYAWK
                     KAQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            complement(51185..52723)
                     /locus_tag="VQ620_000357"
     CDS             complement(51185..52723)
                     /locus_tag="VQ620_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET2763044.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFVHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            complement(52760..52903)
                     /locus_tag="VQ620_000358"
     CDS             complement(52760..52903)
                     /locus_tag="VQ620_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763045.1"
                     /translation="MLTKEEVNDMIEFKRTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            complement(52981..54513)
                     /gene="cls"
                     /locus_tag="VQ620_000359"
     CDS             complement(52981..54513)
                     /gene="cls"
                     /locus_tag="VQ620_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HET2763046.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            complement(54659..55204)
                     /locus_tag="VQ620_000360"
     CDS             complement(54659..55204)
                     /locus_tag="VQ620_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HET2763047.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDID
                     GTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVN
                     GNQIVVDDMDRDPSDQIVLTK"
     gene            complement(55201..56451)
                     /locus_tag="VQ620_000361"
     CDS             complement(55201..56451)
                     /locus_tag="VQ620_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET2763048.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSYREFISDFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            complement(56646..56813)
                     /locus_tag="VQ620_000362"
     CDS             complement(56646..56813)
                     /locus_tag="VQ620_000362"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763049.1"
                     /translation="MERPVDVFSPVPLNKRELVGSGDVFSPAPLNKIELVSLGDCISP
                     TPEMGKSWNVQ"
     gene            complement(56910..57215)
                     /locus_tag="VQ620_000363"
     CDS             complement(56910..57215)
                     /locus_tag="VQ620_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HET2763050.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFVEE"
     gene            complement(57231..57650)
                     /gene="ruvX"
                     /locus_tag="VQ620_000364"
     CDS             complement(57231..57650)
                     /gene="ruvX"
                     /locus_tag="VQ620_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET2763051.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            complement(57654..57920)
                     /locus_tag="VQ620_000365"
     CDS             complement(57654..57920)
                     /locus_tag="VQ620_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HET2763052.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            complement(58007..58576)
                     /locus_tag="VQ620_000366"
     CDS             complement(58007..58576)
                     /locus_tag="VQ620_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2763053.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            complement(58642..59040)
                     /gene="spx"
                     /locus_tag="VQ620_000367"
     CDS             complement(58642..59040)
                     /gene="spx"
                     /locus_tag="VQ620_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HET2763054.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
BASE COUNT        17365 a        13050 c        10506 g        18201 t
ORIGIN      
        1 tcagtaacca tacctacggc aaggcgacgt tgacgtggtt tgaagagatt ttctaagagt
       61 ataacacgaa tcttgagtac aagcaaccaa tttgctcaca agaaaaagcc tagataacta
      121 gactttttta gcttattcta ccgttactga cttggcaagg ttacgtggtt tgtccacatc
      181 gaggccacgg tggagggttg caaagtaagc gactaattgc gttggtacga ccattgaaat
      241 tggtgagagg tatggatgta cggtcgtaag gacgatatcg tcggtatctt tggctacatt
      301 ctcttctgcg atagtgagga ctttggcacc acgggctgcg acctcttgga tatttccacg
      361 agtatggttg gcaagaactg gatctgacaa gagagccaaa acaggcgttc cttcttcaat
      421 caaggcaatg gttccgtgct tgagttctcc tgcagcaaag ccttcacact ggatataaga
      481 aatctctttg agtttgagac ttgcttccat ggctacgtag taatcttgac cacgtccgat
      541 gtaaaaggcg ttacgagttg tttcaagaag ttcacgaacc ttgacttcaa tggtttcttt
      601 ctctgaaaga gttgattcga tagactgagc tacgattgac aattcatgaa ccagatcaaa
      661 gacttgcgct ttagcattac catttgcttc tccgactgct tttgcaagga aggcaagggc
      721 tgcgatttgc gctgtatagg ctttagttga tgccacagca atttcaggac ctgcatgaag
      781 gagcatggta tagttggctt cacgtgagag ggttgaacct ggaacatttg tcactgttaa
      841 gcttggaatt cccatttcat tagccttgac caaaacttga cgactatccg ctgtttcacc
      901 agattggctg ataaagatga agagtggttt cttgctgaga agtggcatac cgtagcccca
      961 ctcagatgag attccaagtt caactggtgt atctgtcaat tcttccaaca ttttcttaga
     1021 agcaaatcct gcatggtaag atgttccagc tgcaaggatg tagatgcggt ctgcgtcttg
     1081 aacagcctta atgatatctg ggtctacgac aacttgacca gcctcatctg tgtaggcttg
     1141 gatgagtttc cgcataacag ttggttgctc gtcaatttcc ttgagcatgt agtaagggta
     1201 agttccctta ccgatatctg acaagtcaag ttcagcagtg tagctagcac gctcacgacg
     1261 atttccatca tagtcttgaa cttccacact atcagccttg acgattacca actcttggtc
     1321 atggatttcc atgtattggt tagtttcacg aatcatagcc atggcgtctg agcagaccat
     1381 gttatagcct tctccaagac caatcaaaag tggtgattta tttttagcta cgtagatgac
     1441 ttcaggatct tgtgagtcaa ccaaggcaaa ggcataagaa ccacggatga tgtgaagggc
     1501 ttttttgaag gcttcaagaa ctgagagccc ttcttcttcc gcaaattttc caatcaaatg
     1561 aacggctatt tcagtatctg tctgcccctt gaagtggtga cctgcaaggt attcttcctt
     1621 gatttcaagg tagttctcaa tcaccccatt atgcaccaag acaaaacgtt ctgtctcaga
     1681 gcggtgtggg tgagcattgt cctcagttgg ttttccgtga gtagcccaac gagtatgtcc
     1741 gataccagtt gttccctcaa caccagctgt cttggcagac aattctgcaa tacgaccaac
     1801 agccttcacc aaatggttat cagcaccatc taggacaaaa attcccgcag aatcatagcc
     1861 acggtattca agcttttcaa gcccttgaat caaaatatca gttgcatttg tgtttccaac
     1921 aacaccaaca attccacaca tagtatatac gacacaggca agctgtgctt tctccttaaa
     1981 attggtatag tctaattcat cttttataga atcagcaaaa acagtatata cttgtttctt
     2041 tcacttgtca agagtaaaaa ttggtatagt tcaaattaag ctcctgtaag caaaaaactc
     2101 tgaccgattg ggataatcag tcagagtcct ttttaaaatc cattattatc gcttaatttt
     2161 ttgaaccagt ggcctgattt cttcagacga cgttcttgcg tttccaagtc taattcgacc
     2221 aaaccatagc gatttttata gctgttgagc catgaccagc agtcaataaa ggtccaaatc
     2281 aagtagccct tacagttggc accatcttca atggcacggt gaagttcacg aagatgacct
     2341 tttacaaagt caatacggta atcatcttga atcattccat cttgacggaa tttttcttcc
     2401 ccttcaacac ccataccatt ctcagtcaac atccattcaa tattgccata attttccttg
     2461 atattttggg cgatgtcata aatcccttgc tcataaatct cccaaccacg gtgagaattg
     2521 attttacgtc caggcatcac ataaggctcg taaaaatgtt ctggtaagag tggactctct
     2581 ggatgcttag caaatcgagg agccataaca cgcaaaggtt gatagtagtt cacaccaagg
     2641 aagtccaccg tattatcacg aatgagttcc aactcttcct ctgtagcatc aggtaaaaga
     2701 ccgtgttcat gcaagatttc taccaactcc tgtggataag tccccaagac agatggatct
     2761 aagaaagatt gggcctgaaa aagggccgca atacgagctg ccttgacatc agcaggatgc
     2821 tggctacgtg gataagccgg tgtcaagttg aggacaatcc caatcttgga atcaggcaaa
     2881 agttcatggc aagccttaac agcccggctg ctggccaatt gtgtatgata ggctacctta
     2941 acagctgcct ctgcatccac cttatgtgga taatgggcat cataaaaata accaaattct
     3001 acaggaacga tgggctcgtt aaaggtaatc cattgatcca ctaaatctcc ataagtctca
     3061 aaacaaaaac gagcatagtc ttcataggct gagactgtcg ccttattttc ccaaccatca
     3121 ccatcctctt gaagggcaaa aggtaaatca aaatgataga gattgactaa cagacgaatt
     3181 cctttagcct taatagcctc aaagacctta cgataaaaat ccacaccttg agggttgact
     3241 tttccacagc cttgtggaaa aatccgtgac cactgaatag aagtccgaaa ggctgtgtga
     3301 ccagtctcta acaaaagctc aatatcccgc tcccaatttt cataaaaagt cgatgtctta
     3361 tctgaaccaa tcccattata gtaacgattt ggctccactt ggaaccagta atcccagaga
     3421 ttgtctccct taccgtcacc agctacacgt ccttctgtct gcggtccaga agtagaggat
     3481 ccccagacaa aatcctttgg aaatcttagc atacatttac ctctttatct actcatttct
     3541 cccattatac agaaaaaaca aggtaaaaac tagttacatt ttttccttgt ttttcttctg
     3601 attatagttt ttatttcttg cttaggattt caagcgtttc aagcacgtta tctgcatgaa
     3661 cctcaatggt gtcaccagtt gccttgatct taacttctac aatgccatcg gccgcttttt
     3721 taccaacagt gatacggatt ggaagaccaa tcaagtcact atcgctaaat ttaacaccga
     3781 cacgttcgtt acggtcatct gtcaagactt cataaccagc tcccatcaag cttgcttcaa
     3841 gtttttctgt caaggcttgc gcttcttcat ccttgacatt gacagtaatc aaatgcacat
     3901 caaatggtgc caattcttta gggaaattga ttccccaagc gtaacggtat tcaccttttg
     3961 gcgttttgtt aacaaagagg cgagcgtgtt gctccatcac tgctgaaaga agacggctga
     4021 caccgatacc gtaacatccc atgatgattg gcacagcacg accattttca tccaagacat
     4081 ctgctcccat gcttgctgaa tagcgagttc cgagtttgaa gatatgaccg atctcaatac
     4141 cacgcgcaaa gttaaggaca ccttgtccat ctggggaaat ttcaccctca cgaacttcac
     4201 ggatatccac atattctgca gtaaaatcac ggcctgggtt cacaccagtc aagtggtagc
     4261 catcttcgtt agcaccgaca actgcattgc gaacatcttg taccttacga tctgcaataa
     4321 ttttaatatt ctctggcaaa ccaactggtc caagtgaacc aaatcctgct tgaacaacat
     4381 tcgccacttc ttcttcgcta gcaacgtcaa agaaatctgc tcccaagtga tttttcaact
     4441 tgacttcgtt aagttggtca tttccaacta gaagggctgc aacaagctcg ccatctgcaa
     4501 tgtagaagag ggttttaatc gtttgttctt ctggaacatt gaggaaggct gcaacttcat
     4561 caattgattt aacatctggc gttgcaacac gagtaacttc ttcttcagcg acaacacggt
     4621 tgcttggttt gtactcgttt gttgccattt ctaagttagc tgcatagcta gactcacttg
     4681 agtaagcaat ggtatcttca ccagagacta tccatttgag caattctgcc ttgatttctt
     4741 cttgcacttc tgcaggaatt tcgtcaaatg aggcaactga cttgtccaag acaacccagc
     4801 ggtcaaggtc tgtacgagca gatgtaatgg ccataaattc ttggctatcc ttaccaccca
     4861 tggctccacc gtcaccaata atagccttga agtctaaacc actacgagtg aaaatacgct
     4921 cataggctgc tttgtactca tcataaacac tatccaaact atcatagtta gcgtggaaac
     4981 tataagcatc cttcatgata aactcacgtg tacgaagaag tccattacgt gggcgttttt
     5041 catcacgata cttgggctga atttgataaa ggttgagtgg caattgcttg taagatttaa
     5101 cagaatcacg gacaatagct gtaaaggttt cttcatgagt tggacctaag ataaagtctg
     5161 atttttcacg gttttttagt ttgtaaaggt cttcaccata ggtttcgtaa cgacctgatt
     5221 cacgccacaa ttctgcacta agaagggcag gagccaacat ctcaacagca ccaatctttt
     5281 cgaattcttg gcgcatgatg tttttagctt tttcaatcac acggttggca agtggtagat
     5341 aagaataaac acctgctgaa acttggcgaa cataaccagc acgcaacata agagcatggc
     5401 tgataacttg agcatcgctt ggcatttcgc gaagcgttgg gataggcatt ttactttgtt
     5461 tcataatatt cctcgattat ctaaaaaaga gtcgcataat gtcattccaa gtcacagcaa
     5521 tcatcaagac aaccatgatg accactccgg ccaaggtgac ataggtttca atttcttgtt
     5581 tcaatggttt gcggcggatg gcttctagga tattgagcac aatcttacca ccatccaagg
     5641 ctggaatcgg aataagatta aaaatcccaa tattgatgga aatcattgcc aagaagtaca
     5701 agatattctc aattccattt ttagcagcat cactacttgc cttaaagata gcaacaggtc
     5761 cacccaactt gttcaaatcc ggttggaaaa tcagattttt cagagctgag agaattcgga
     5821 gagctgagtc agcagcagtt gtaaaaccac ctacaaacat ggatagaaaa tctgacttaa
     5881 cccccggttg aacacctaga aggtaacgac cttgactatc ttcgggtgta acagtgactt
     5941 gtttgtcact ccccttttca gaaatagtca catccaaagt cggtgccgtc ttatctttgg
     6001 tttctgtttc cacagcttgg atcaagcttt cccagttgct aacctcatgt gagccaatct
     6061 tggtaatttg tgccgtttct ggtactccta ccttggccaa ggcaccttgg ggcatgatat
     6121 ggaactgatt ggtatcaaca tctctgacac caccctgcat aaagattaaa acccaaaaaa
     6181 caacgacacc taagataaaa ttgttcatag gacctgcaaa attggtaatc agtttgcccc
     6241 agatagtcgc attttgatat tgaacatcta aaggtgcaat ccgaacctca gtaccatctg
     6301 cttccacaac cgttgcatcg tgatccactg caaatgtttt ttcttcttcc agaaccaatc
     6361 ctttgataaa gagcttgtct tcaaaatcaa actgggtcac ctgcataggg agggctgttt
     6421 gatccaattt tttacctgag agattgatgc gtttaacctt accatcatca gcaagtgtca
     6481 aactaacagg cgttcctgtc ttgatttcag ttgtatcatc accccaaccg gccatgcgga
     6541 catagccacc cagaggcaag attcgaatgg tataggccgt tccatccttg ccaatgtgag
     6601 caaagatttt aggtcccata ccgatggcaa attcacgtac taaaatccct gatttcttgg
     6661 caaagtagaa gtgcccgaac tcgtgcacca ctacaataat cccaaaaacc agaataaagg
     6721 ttaaaattcc gagcatagcg tttcctccgt cttttgatta aaagagtcca aataagtgca
     6781 tgattggaaa tacaagcaac atactatcga aacgatccaa aacaccacca tgtccaggga
     6841 taaatttccc agaatcctta acaccaaaat gacgtttgat cgaactttct agtaaatcac
     6901 caaattgtcc agcaatgcta aagaaaatag caaagactga catcttgtaa attccatatg
     6961 gaagagcaac tgtactgtca actatcataa agataatggt tactaaaatt gctcctaaaa
     7021 taccacccaa ggcaccctca agggttttat taggcgatac ccttggtgct aactttcgtt
     7081 tcccatagtt catcccaaca agataggcac cactgtctgt cgcccagacg atacacaagg
     7141 ctaagagagc cttgtccaaa cctgcaacac gagcatctag taaagcatta aatccaaagc
     7201 ccacgtagaa gctcatagca agagggaaaa ccgcatcctc aatcgtataa gacttgctaa
     7261 aaacggtcgt tcctaacatg attgaaatca aaacactata ggcaaccaca ttcccatcaa
     7321 ctggcaaaaa agtcaggtaa ttctccaagg gaatggtcaa tgcaaaggtt gcaaagaggg
     7381 tcaagaggcc ctccatcgtc atggtctcta gacctctcat cttcaaaagt tcatgcatgg
     7441 ctagcatggc tatgattccg attgctatct gaagcaagag gcccccaatc attaaaattg
     7501 gtaggaaaat agccagggca atccctgcaa acaaggttct tttctgtaaa tcctgggtca
     7561 tatttcctcc taaactcctc caaatcggcg atgacgacga ttataggcaa gaatagcttc
     7621 ctgcaaggcc gcttcgtcaa aatcaggcca taaggtgtcc gtaaaataaa gctcactata
     7681 ggctccctgc catggaagga aattgctcaa acgtaattct ccactagtac ggataatcaa
     7741 gtctgggtct cgtaagtcct taggcaaatg ctgagtaaag agatagttac caatcaattc
     7801 ctctgtaatg tcacctgggt tgattttggc atctaaaaca tcctgggaaa tcaacttaag
     7861 cgcctgtgta atctcagcac gtccaccata gttaagagca aaattaagaa tcaatcctgt
     7921 gttgttctta gtcaattcct cagccttggt taaagcttca aaggtttgct taggcaggcg
     7981 gtctgtctcc ccaatcattt gaatcttaac attattcgca tgtagttccg ggacataatt
     8041 atcataaaac tctactggca agttcatgat aaacttgact tcctgatctg gacgggtcca
     8101 gttttccgta gaaaaagcat agaccgtaat aaccttgacg cccagtttgt tggctgcctt
     8161 ggtcacggtt tgcaatgctt ccatgcccgc cttatgtcca aaaactcgcg gttgcatacg
     8221 ttttttagcc caacggccat tgccatccat gatgatgccg atatgagcag gaacctgtgt
     8281 cggaacctct acttccacag ccttatcttt cttaaaaagt ccaaacatga tcttattcct
     8341 attcaaaaat ctatcgtttc attataccat atttccccat tttcttctat cactaagcta
     8401 tttattctca ggcaccatgc ccatttttca aaaaaataag ccgcctgatt aggcgacttt
     8461 atttttatag ggagattatt atgaaaaagt tttaggagtt taagttaagg tcttcttaac
     8521 ttatgaactt agtgtacact ccctagctta aagtttcctt aagtattttt aaaaatcaaa
     8581 tttttccatt tctcctgcca atttttcttg gataaacgtg tttgatagag ttccattcgg
     8641 tcttcatttt ctaagaaatg aggagttgga cgaacttgaa aattcaaaat atcctccaaa
     8701 ccataaggtg catagagttc aaaatctgat tcttcattca agcgcagtcc aactgccgta
     8761 caccgttctg gatacttact catagcatca cgagaactgg tatagaaagc agtgtgagga
     8821 ctgtgctggt gcatatagac ctgatttttc aattcccaat ggtactgagg aaaatcctct
     8881 ctcagctttt tctccagtaa taaggtttcc tcataagaaa tatccggatc aaaaaaaatc
     8941 acatctacat ctgtttcaaa gtcaaaaggt gatttgtctg acaagagatt ccagatgaaa
     9001 tttctgacag aacctgctgc caaccaggaa tctgtcaact ccaggttttg gatgatagtc
     9061 agaatagcca tcatatctgg attttttcta aaagcctcta gaatttcttg cttatttttc
     9121 attgtactca taccccaaat gctcatatgc cttagcagtc gccatccgtc cagaccgtgt
     9181 ccgcatgata aaaccttttt gaatcaagta aggctcatac atgtcttcta cagtctcacg
     9241 ctcctcggca atattgacag aaagagttcc taatccgaca gggccaccac cgtacatctc
     9301 aatcatggtg cgaaggattt tttgatccac atagtccaaa ccttcatggt caacatccag
     9361 catagtcaaa gccttatcgg taataacatc atcgataacc ccattcccca ttatctgggc
     9421 aaaatcgcgc acgcgcttga ggagacgatt ggcaatacga ggggtcccac gactacgtag
     9481 ggccaactca gatgctgcct catgagtgat ttccatctca aaaatatctg ccgtccgctc
     9541 gacaatttct gtcaagtcag catgggcata atactccata tggcctgtaa tcccaaaacg
     9601 tgcccgtagt ggatttgaga gcataccagc ccgagtcgtc gcaccaatca aggtaaaagg
     9661 tggtaactcc aaatgaacac tacgactacc ctcaccagcc ccaatcataa tatctatgta
     9721 gaagtcctcc atagcactat aaagcacctc ttccactgac attggcaaac gatggatctc
     9781 gtcaataaag aggacatctc caggctctaa ctcattcaaa atcgctacca aatcacccgc
     9841 tttttcgata acaggaccag acgtttgctt gagattgact cccagttcat tggcaatgac
     9901 aaaagccatg gttgttttcc caagccctgg agggccaaat aagagcacat gatccagcgc
     9961 ttcatcccgc attttagcgg cttcgataaa gatctgaagc tgatccttaa ccttatcctg
    10021 accaatatat tcacgtaaat actgaggacg gagcgtgcgt tctactaact cctcatcacc
    10081 catcatctca ttatctaaaa ttctactcat ggctctatta tatcaaaaaa acaagccaca
    10141 aacaaaaaag ccacctgatt gggtgactcc taagtttagc acttatgtga tataatattc
    10201 tacgacactg ctacaccgcc tacgaaagga ggtgagatag cccatgatgg aattagtact
    10261 caaaactatc atcggaccaa ttgtggtcgg tgtcgttctt cgtatagtcg ataaatggct
    10321 aaacaaggac aaatagtgtc aaaaaagacc tcaagcttat ttggtcgtga gcttgggatc
    10381 ttttctagcc tatgatatag aactagtact caattccttt ttattatccc atagttcacg
    10441 aattttgtca aaggtttaca ttttcttcaa ccgctgtacg acaagacggt taagattaag
    10501 agaacgttag ggattctatc aatttcatag aaattttgat ttcgtaaacg aagagacaat
    10561 cttacatgtc acttctcatt taatacgcca ctactagaca agcaaaatca ttattacagt
    10621 agttccagtt cttcaattaa cagtcactta caatcaaatt gagtttgaac tagctgaagc
    10681 gaccacagac ctatttctta gttatattcg ctaaaaaagt ccccgccaat tccccgacca
    10741 aaatccgaaa aataccgaaa aatatcgaaa aattattttt agaatagtcc ccaaaagcct
    10801 gaaatagagc taaaaaactc cacctgattg ggtgaagtta agggagatta ttatgaaaaa
    10861 gaaaagttta ggattttatt aaataaagtt aggaggtctt tatttaataa ctacatgata
    10921 caagacgaaa cttaaaacta gcttaacttt tctaaaattt tactattttg caaaaaattt
    10981 ctatcaccag cacctcacca cgcaaaaaag ccacctgatt gggtggcttc attaggagat
    11041 tgtgatgaaa aaagtttagg attttattaa ataaagttag gaggtcttta tttaatgact
    11101 atataatact agaccatcct taaattttgc ttaagaaaaa acaagttttg ttatttttta
    11161 cgattcatcc aagtcatccc tatacaatta taggtggata agatttcaaa gcccatagaa
    11221 cgatagaagc ccaaggttct ttctgtctct tctgtcacca gctggacttg ataggcatct
    11281 ttgtaatcct ctaaagcctc tttcatcaag gcactaccaa ttccttgacg ctgatagatt
    11341 ggtaaaacga ttaaatcctg aaccaatact gatgagaatc catctccaac caaacgaatc
    11401 aagcccacca cagcatcacc atcaagtgcc agataaatta ctaatgaatg agataaggcc
    11461 tgctccagca tctctggttg atgggtataa tttgtccaac cgacagcctg atagagatgt
    11521 aaaacatcct ctagcttgac aatttcttgc tttttaatag taatcatctc aacacctctt
    11581 aaagttctct caagctcttg tactgctgtc catttttatc aaaattttca ggacgcaacc
    11641 atgtttccaa acgatctttg acttgaggcc agtccttatc aatcatagac aaccaatccg
    11701 tatctctcgt acgtccctta taaaccactg cctgacggaa ggttccttca taaataaagc
    11761 tcaaacgctc cgcagctcgt ctggatggca gatttaaggc atcgcatttc cactcgtagc
    11821 gacgatagtt tagctcctca aaaacgtagc gagctaggag atactgagct tcagttccta
    11881 tccgtgtacc ccttagctct ggagaaaaag tgactgctcc gacttctatt actcggttat
    11941 tctggtcaat gcgcatgaga gaaaaagatc ccaaagcctt accagtcgcc ttgtctatga
    12001 ttgcatagta aaaacggtcc ttacgagcca acatctgatg taaaaggcta accagctccc
    12061 ccatatctgc tactggctcc tgaaaaaggt aggtccacat ctcctgaggc gtatcaggcc
    12121 cataaacagc tagcaaatcc tccgcatgct tttccactga aagagcctct atcctagcat
    12181 aacgcccttc taagaaatca atagaaggca gttcacctgg tgtataacct tccattgact
    12241 ccccaatcat ttgaccatat tcatttactg gcatagcttt ctcctttttt cacttttcaa
    12301 actttatagt tgcccatagt ataccatact ttcataagaa gattcaaaaa atcctccaga
    12361 ttctactctg aaggattcct accttatttc acaacgatat taacgagttt agttggctca
    12421 aacagtttgg gaaactgttt gaggttggaa atcagcaaat aaattggtta ttttacaaca
    12481 atattaacca atttatttgg tacactaatc actttcacga tttccttacc gtcaatttct
    12541 gctttgactt tttcatccgc tagagcaatt tcttgcaatt cttcacgtga taggtcttta
    12601 gcgaccatga gtttggcacg gactttacct ttgatttgga caacgatttc gatttcgtct
    12661 tcaaccagtt tactttcgtc ccaagttggc caagctacat aagagattga ctcacctgtt
    12721 tctgcgactg tttgccagag ttcttctgcc aagtgaggtg caaatggtgc aatcaattga
    12781 ataaagcctt tggcatagtc aacataaagc ttatcttcct tgttagcagc attgacaaag
    12841 accataagtt gggcaatagc tgtgttgaat ttgagagact caatttgctc agtaacagct
    12901 ttgactgttt cgttgtaagc cttgtcaaga gcaccattgt tttccgcaag gatttcttta
    12961 cttgtaatca aacggtaaac tcggtcaagg aacttacggc ttccttccaa accttcttct
    13021 gaccaagcaa tcgaagcatc gagtggtccc ataaacattt cataaacacg aagggtatcg
    13081 gcaccgtatt gttccaccac atcgtctggg ttaacaacgt tcttgagcga tttagacatc
    13141 ttggctggcg cttgctccaa ctcttcccct gtttctacat ggaagaagga accatcacgt
    13201 ttttcaacct tgtcggttgc cacaagagca ccacggtggt cacggtagct tgttcccaaa
    13261 atcatccctt ggttaaagag tttttggaat ggttccttag tcggaacaac accgaggtca
    13321 tagaggaatt tgtgccagaa acgagcgtaa agcaagtgaa gtacagcatg ttccgcacca
    13381 cccacgtaga tatctactgg caaccattgt ttgaggaggt cctcatcagc caatttctca
    13441 gtattgtgtg gatcaatata gcggaggtag taccaacttg aaccagccca ttgtggcata
    13501 gtgttggttt cacgacgacc tttgacacca tcttcacgag tcacttcaag ccaatctgtc
    13561 aagttagcta gtggactttc accagtacct gaaggacgga tatccttggt tacaggcaag
    13621 acaagcggca attcagtttc aggaacagct gttgaagttc catcttccca atgaatgatt
    13681 ggaattggct caccccagta acgttgacgg ctaaagagcc agtcgcggag acggtaggta
    13741 accttctcct gaccacagcc tttttcttcc aaccaagcca caatcttggc aatagcgtct
    13801 tctttgttga gttcatctag gaagtctgaa ttgacatgca ggccatcctc tgtgtaggca
    13861 gcttctttga catctccacc ttcaagtact tcgacgattg gaaggtcaaa ttgtttggca
    13921 aattcccagt cacgttggtc gtgggcaggc acagccataa ccgcacctgt tccataacta
    13981 gcaagaacat agtccgcaat ccagattggc atttccttac cattgacagg gttgatggca
    14041 taggcacctg tccaaacacc agttttttcc ttagcaaggt ctgtacgagc caagtcagac
    14101 ttaaggctgg cttggtgttt atagtctgct acagcttctg cttgctctga acttgtgata
    14161 gcgtctacta attcatgttc aggagccaag acagtgaaag tcgcaccgaa aagtgtgtct
    14221 ggacgagtag taaagactgt aaattccttg tctgttcctt ttactttgaa agttacattg
    14281 gcaccagttg atttaccaat ccagttgcgt tgcatatcct tgatagactc tgaccaatct
    14341 agttcatcta agtcattgag caagcgctct gcgtaagccg tgattttgag catccattgg
    14401 cgcattggtt tgcggacaac tggatagcct ccacgctcag aagttccgtc aggaagcact
    14461 tcttcattgg caatggcagt tcccaattcc tcaacccagt ttactggcac ttcagcttca
    14521 taggccaagc ctttttcgta aagcttggtg aaaatccatt gagtccactt gtagtagttt
    14581 ggatctgttg tgttgacttc acgatcccag tcataagaaa atccaagcgc attgatttga
    14641 cgtttgaagt tggcaatgtt ttccgctgta aattctgctg ggtcattacc agtatccata
    14701 gcgtattgct ctgcaggtaa accaaaagca tcccaaccca ttgggtgaag gacattgtag
    14761 ccttgcgcac gtttgtaacg actgaggata tcggttgcag tataaccttc tgggtgtcct
    14821 acatgcagac cagctccaga cggataaggg aacatatcaa gcgcataaaa cttaggtttt
    14881 gatgcatctg ttcctgtctt aaatgtatga tgttctgccc agtagccctg ccacttaggc
    14941 tcaatttctt tatgattata aaaactcatg gtctctctcc aattttgtga tgttactatt
    15001 ataccatttt tgagggaatt tgaaatgtgt ttaaagaaaa aactgtatac tgtatctcat
    15061 taattttgat gactatgaat tgctctatct cccacttttt atcccattgt tatgcagtaa
    15121 atcaaggagt aagtcaagaa gcttatctcg tatagcacaa aatacttact ttagaccgaa
    15181 gataaaactg gaatgctaat taatgtattc acaataaata gccgacaacc tatgtcgctt
    15241 tttgatttat gaagaacgta tagtggactg aatctgaaat agtacgaaac aattgctaaa
    15301 acacttatag aaattaattt tactttccca atcgatttgt tctcatctta tttcaatctg
    15361 ctatagaatt caatacgagg atcaataaaa gactaccatt tggaaaacag tagtccttat
    15421 tgtttatttt gaatttacat aggcatctac agcgataata gcttgagcaa catctgaagg
    15481 cgaaatctta aacggcatct gatgaattgt ctcaccctcc atagttgctt gtttaccaac
    15541 ttttattaaa tcatcatatc caacttgatc caaatgcatt tcttttagag ttgttggcat
    15601 accaattttt ttgtaaaact caatatactt atcaagttct tctttaggtc tattttccaa
    15661 taatagttgt actaaagttc cataagctac tttttcacca tgtgttaaat gatgaatgtc
    15721 acctgtcaat gcagtaaaac cattatgaat tgcatgcgcc gcagctaatc ctccactttc
    15781 aaaacctaaa ccactcaata aagtattagc ttcaacaatg ttttctaatg ctggtgtcac
    15841 cactttagct tcacaagctg ccatagcctg taaaccatct gcaaacagcg tttcttcaca
    15901 tttcttcgca attgcaactc cagccaatgt ttgctgttgt cccaacatag tttttccatt
    15961 tgcctgcata accgcacgcg cctcaaccca agttgctaaa ccatctgcaa taccagacgc
    16021 taataaacgc ttaggggctt gtgaaataac ttttgtatca accaaaacta aatctggatt
    16081 tttagaataa aatagataat gatcaaatgc accttcatct gtataaataa cagataaagc
    16141 agatacaggt gcgtcggtcg atgcaattgt tggagcaata ataacaggct tttcaatcaa
    16201 atctgcaata gcttttgcgc tatcaatcgt ctttcctcca ccaagaccga taatactatc
    16261 acaattttct ttctcagcca aggcaacaac tcgattgatt tcattgtcag aagcttcacc
    16321 attaaatagc gccagaacaa tatggaaacc atacctatgt aggtaatctt caaatcgttt
    16381 tccaacaata tcataaacca actgatcgca taatagaata gggcaatttc ccaaatccaa
    16441 aattgatttg gcattttcaa acaaggcatt ttccccctga atatatctag aaggactagc
    16501 aaaaattctc atatgtattt cctctctatc taaatggaac gactattttg aataacaaac
    16561 caatcgtcag aaaaatcatc aaccgccttt tggatagatg gcatggcgaa agctgattca
    16621 aaaacatctg ctcctgctgt aacagcatgc gcacctgcag ctaaagcatt atttacttgt
    16681 gctacatttt taaaggatgc agctaaaatc ttactaggag agttctgtct atcaatagca
    16741 agagctaatt gacgaatgac agaatttgaa tcaatgttca gattttccat tctattataa
    16801 tatggagcta ggtaatccgc tcctgcttcg atagctaata atccctgaat aactgtataa
    16861 atagctgttg cagtgatatg gtagccctct ttttttagca cctttattgc acgtaatcca
    16921 gccggagtaa caggtacttt gataaatata tcatctcctg cttgtcttcg aatttcatga
    16981 gcatccttta agatgccttc aaaatcttga gaaatcacct gaacatgaat agagggtgta
    17041 gagccaatca attctcttac atctttgatt cgttcaaaaa aattaataga accctctctt
    17101 tttgcaatag tgggatttga agttacccca gctagcggca aaatttcaga ccacttttta
    17161 atctcatcta aatttaatgt gtcaagcata aattccataa aaagaacctc tttattttac
    17221 aatgtatgct cagtacgtcc tataatgtca tcttgggttg ccttagaaag aacattgaag
    17281 aatgcagagt atcctgcaac acgaacaatt aagtctctgt gtttttcagg atgtttctga
    17341 gcgtcaatca acgtctctct ggaaacaaca ttgtattgaa tatggtaccc atgtaaacga
    17401 ttaaagaatg ttcgtaacaa agcaattagt tttaatttat cttcttcttt ggctaacgtt
    17461 tgaggattta ctttctgatt taagagaacc ccacctacga tttcatctgt tggtaatttt
    17521 gaaacagatt ttaaaacaga tgtagggccg tgttgatcca tattatgtga tggtgaacaa
    17581 ccctctgcta acggtgtacc cgcgttgcgt ccatctggcg ttgctaatgt tccacgtccc
    17641 tgccctacgt tggctgagat agaagatgtt cctgaataac gaattcctcc aataggacct
    17701 cttccataac gtgtattagg atatttagca atttcatcaa cataaatgtc ataagcagca
    17761 gtaaccaatt tgtcagcata atcatcatca ttaccatact taggtgcatc atgaatcaac
    17821 atttcttgaa tgaccttacc ttcttctccg gcataatctg tttccaatgc atgccaaagc
    17881 tgacttgggc ttatacgttc ttcctcaaac accaattttt taattgcagc taatgaatcc
    17941 gacaaatttg caattccaac ttgcaatcct gatatataat catatactgc tccaccttct
    18001 ttaaggtgtt ttccacgacc aatacaatca tcagtcaatg ctgaacatag aatatcagga
    18061 acttctcgtt ccaatgataa atcaatagaa ttttcaacaa taacactcat tcgtgtcaaa
    18121 tatcttagtg ttttatccca agcattttct aattcagaaa agttcttcat atccttaaaa
    18181 tgaccaaagc ttggtgcaaa ccgtttaccc gaagccggat caattccatc attcatcgtg
    18241 ataagtagaa ccttagggaa gttcatataa ctcatacctg tgcaacgata gccccatttc
    18301 cctggaactg ccgtttcaac acatccaatg gcactgtaat cataagcatc atcttccaat
    18361 actccttttg caataaaaga aggaataata atctcatcat tattaaatgc aggcatacca
    18421 aaaccaagtt tcatcacttc aatacactca ttcatgaaac gagcatctaa acctgcatgg
    18481 taacgtacag ttagattagg ttgcggtaga tgggtttgtg caactgattt taataccaaa
    18541 taagataatg ggttaacagc atccttctta tctcgagtct gtccaccaat tgtaacattt
    18601 tgatataaag gacttcctgc tgaagaaaat gtatgtgatt gactgcgaac cttattaatt
    18661 gtaattgtct taatccaaag atttgtcaga cgttcaacaa tgctatcttc tgtttcttta
    18721 ccactttcta aatcagcctt catatatgga tacatatatt gatcaaaacg gccatatgaa
    18781 agagagtggc cattagattc aatttgtaaa atacattgaa taaaccaaac tgattgaata
    18841 gcttctgcaa aagtagttgc cggttcatat gggactctag agcaaatatc tgcaatctca
    18901 agtaattctt tcttacgttt aggatttgca ttttcggcta aacttttagc aagagcaaca
    18961 aagcgctttg catatacttt aatagcatcg attacgataa atatagagtc gtaaaaatga
    19021 tatttatcaa tacttgctgg atctgttaaa tctagagcta cttttgcttt acgagcccgc
    19081 tcttcaaaac ctcttaaacc aaattgcaaa agtttctgat agttaactgc taagtgagca
    19141 tctccagaat tcatcttacc ttccataccg aagaatcctg tttccatata aacagacact
    19201 tcttcaggta ataaggcacc agctctagca cgtaaattat tattttccca aaacggagca
    19261 atacttctaa gttgttcttt tgtttcttct gtaatataga aaacatctcc atcacgcttt
    19321 tcaaaaagat ccaactcatt gagaacaaat tctagcgtat attccggaaa aataggagca
    19381 tctttattgg aagaagcttg atttcccgca atcatagatt cttcttcaat atagatagtc
    19441 atattttcca aaatttcttt cagcatatat gcacgtttta ggacattagg ttgttccttg
    19501 tatcgttcat aggcgcgtgt tgctagaact gctctctcag catcaatata aggtttttta
    19561 tttaaaacat cttctcgata tttgttcatc ctttcagtaa ggctgccaaa atattctgtc
    19621 ttgattgttg ttcttgctac ttctgtattt accatcgtaa gcacctttct ttcgaaaata
    19681 tttttatttc tttcgaaaat ataataccga tttttataga aattttcaag cttttaacta
    19741 gaaaaatttt aactctctaa tttttagcac aaatgtgtga gtttaacagt aacacacaaa
    19801 tacctctaag attgtttgat ttcattttgg taagctaaac gatcctgtat cttaaagaat
    19861 ggaaagtaaa ccattgtaga catcgccaat actaataact gagtcacaac accttgccat
    19921 ccacccacca aaaatcctga tagaatagct ggtgtactcc aaggcaatgt tacccctgag
    19981 aatggctgca tgaaacctgt tgcaatagct ccatatacta tcacagctgc aagtacagga
    20041 acaagaatga aaggtacaaa cataactgga ttcatgacaa tcggaaatcc aaatacaact
    20101 ggctcattta cgttaaatat tgctggaaaa gctgcaactt ttcctaaggc ttggtattgt
    20161 tttgattttg ctacaaaaag catggcaact acaagaccaa acgtaatccc tgaacctgat
    20221 agaattaaaa atgaatctaa aaactgttga gtaacaatat gtgctccatt ttctaatgat
    20281 agattaccag aggctaacat agctttatta gcatcaagat tagataaaag cagagctgtc
    20341 actactccat ttactaccga ttgcccatga acaccaaacc accacaaaaa tgatataaag
    20401 aatgcaattc caatagcacc atacaaagat ccagttaaac cttgcaacgg aacttgaata
    20461 gcagaataaa tcatttctat gaatgttccg ccattagtca ttgacttcgc taaaatatat
    20521 acaatcatag aagataagaa aattacaaat gctggaatca ttgcttcaaa ctgtttggca
    20581 atagcttgtg gaacttgttc tggcatctta ataacaattt ttctctttat aaagaaggta
    20641 taaatacttc ctactaccaa acctataatg atagcaccga taattccttg gcctccaaac
    20701 caaactttac taatagcgtc cccaatcgcc tcaccttgtt tagggatata agatgatctt
    20761 agcaaaataa agaatgcaga tacagataga actccagctg gtaaagcctc tactccgcta
    20821 ttcttagcat aagaataggc aattgaaaaa caagaaatta gacccataat agcaaaagtt
    20881 cctgaatata cttgcataaa cggctctgtc caattagctc caaaaacact agcaatgctc
    20941 ttatttaatc cttcgaacgg caattgtccc ataatcaaga ataaactacc aactactgtc
    21001 aatggcaaaa ttgctaacat cccatctttt agagctataa tgccacgcat attcacaaac
    21061 ttcatcatcg gtgcaatgat tttctgaaca tccatctttg acataataaa tctccttttc
    21121 ttacccacta atcaaagata gggccaaatc taatactttt ttcccatcta acataccata
    21181 gtccatcatc ggaataacag ctatcggaac atcacactta tcacaaattt cttttgattt
    21241 atctaatgta taagcaactt gtggacccaa tagtgcaaca tctatatttg gcgcataatc
    21301 cgctaattta gactgagaaa acgcctctat ttctgcctca actccactag cttgcgctgc
    21361 aattttcata ttatttacaa gcataccagt agaaaaacct gctgcacaaa acaaaccaat
    21421 cttcaccatt atgttttcct cctctatgtt aataacaatg ataatactct agtattattt
    21481 tttatgaagt tcttttctca aactaataat ttctttgatt aaattaatct ccgtcatact
    21541 agtcatgaga tgatcttgtg aatgaacaaa gagagctgta atttctgtct ttgtaccaga
    21601 cgcttcctgt gccaagaatt ttgtttgtag attatgagct tctaataagg ctgcatcagc
    21661 taactggatt tcttcttcac atcgttcaca tgtaccgttc tttatgtaat ctaatgcttt
    21721 ataaatatgt tgtttcgcat cgccagcata taaaattaaa cccatgataa tttgatctgg
    21781 aacaataatt tccataaatt cctcctcatt tcactatgta aaaagctttt ttcatttgaa
    21841 attttaaaat tttcttttgt aactatagaa tacaactaca taataaagtt gtcaatccaa
    21901 aaacaacaga taaactattt ttgtttatat aagcacaaat gtgcaatcaa ctaaaaagaa
    21961 aaacgataaa ctgaatcagt ctatcatttt cactcaccta gaatctctct aaaagtcaaa
    22021 tccccatctt cttccaaaac ttttaaaatt gtatttttgt ctgtaatcaa atgatttact
    22081 aaattagcac gcaagacaga aagaatcgaa gatactttcg tatcaccgta agcaacagct
    22141 aaactttgag gaatattttt taaatcttcc aaagagatgg ctatcgttct ttcctgtaag
    22201 ttttcataga cttctttacc ttttgaatca aaaaatcgac aacaaatttc tcctacagtt
    22261 ttaactttgg tcaattcttt aaagtcatcc tctgtaagca tgtctaacca ttgatgcttt
    22321 cctttattgc taaaatcacc aattccgact acagctatat ctaaatcttt ccaactattt
    22381 ttcaaatttt caaaatatct tgattgcaaa ataccatctg ctaacaattt attttcttgc
    22441 acaatcgttg cattcataaa tgtacactct ccatgaaatt ttctagacat ttcataaatc
    22501 agtgtattca catggtattt agcgtgtatg tgactaggac cacctgctag aggatagaag
    22561 tgaacatttc ggacactttt actgtgaatt aaatctacta aattacttaa acttttcccc
    22621 caagaaaagc caattttcat attatcatca attagattcc taaggacacc tgctgcaact
    22681 tgagaaattc tttcagataa aattgttgga ttatcatcaa attcatttgg aataatttct
    22741 aaactttcca gaccatattt ttcttttaca taattttcca acttaaacat attggtatca
    22801 aaattctcta tttcaatttt aacaattcct gcattccttg cttctgttaa cattctacta
    22861 atagaggttc tataaattcc taattttgct gctatttgtg actgatttaa gttttcaata
    22921 taatacagat aagcaatttt agagagcagt ttattcctat cttgattcat acatttaacc
    22981 tcttacgaaa ctaccttaac cattatccca gcattttcta atgtagctat attttgttta
    23041 gaaagttttt cgtctgttat tacttcatag acttgactta aagcaaatct tcttactgta
    23101 ccccttttat caaatttact tgagtcagtt agtacaatga ctttatccga cgctgctgaa
    23161 atatattgaa ctacctcact gcgcattaaa tcttttcctg taaagcccat ctctttatcg
    23221 taaccatctg tcccaacaaa agcttgatgc acatgaaaag tctgtatcat ttcttttaat
    23281 aaaggtccca cagtaacctg tgaatccttc tgaaactctc caccaagaac aataatacga
    23341 catgaatcat aagctctcac aaaatttgct ataaaaaatg aatttgttac aatcgtaaca
    23401 tttctttttt gcttgcaaat ttcctcagca agtaaagcac aggtcgatcc agattctatc
    23461 attattgttt cattatctga caccaatttt actgcttcct gaacaatttt tctcttaatt
    23521 tcatagttaa ttgacaaacg tacatttaag tcatctccac tatttaatac agcatatcca
    23581 tgctctctgt gtaataaacc ttttgactct aatttatcta aatcttttct aatcgttact
    23641 ttcgatacat ttaatttttc cgataatgta ttaacgtcga tcttttcata ttctgatact
    23701 aatttaataa tttgttccaa tcttttcatt ttacacctcc gttttattct accaaaataa
    23761 aaagcaaaaa acaacaaatt aaactttcgt tcgtaattgt ttttctttcg tttttgtgat
    23821 aggatagact tatgaagagg aggaactctt atggaaatat ctaaaggaat tatttttaat
    23881 attcaacact tttcaattca tgacggcccg ggtattcgta caactgtttt tttaaaagga
    23941 tgtcctctgc gctgtccatg gtgttctaat cctgaatctc aaagaatgaa acctgaaaaa
    24001 atgaaagatg ctcaacgaga gaaattcacc ttagtcggtg aagaaaagac tgtagaagaa
    24061 attattacag aggtattaaa agacaaagaa ttttacgaag aatccggtgg aggtttaact
    24121 ttatcaggag gtgaaatatt tgctcagttt gaatttgcta aagccatctt aaaatcagct
    24181 aaagaacatc acatacacac tgccattgaa actactgcct ttgttgatca tgaaaaattt
    24241 attgatttaa ttcaatatgt ggattttatc tacacagacc taaaacatta taattctata
    24301 aaacataaaa aagtgactgg ggtttttaat caaatgatta ttaaaaacat tcattatgct
    24361 ttttctcaaa ataaaactat cgttttaaga atcccagtta ttcctaattt taacaatagt
    24421 ttagaggatg cagaaaaatt cgctactcta tttaactcat taaatatcga ccaagttcaa
    24481 ctactccctt ttcatcaatt tggtgaaaac aaatatcgtt tattaaatcg gaaatatgaa
    24541 atggatggaa tcaacgcact tcatcctgaa gatcttattg attatcaaaa ggtatttctg
    24601 aaccaccata ttaattgtta tttctagttt atttccttga aatgctctag ctatttgcag
    24661 ataacaagca tctatataag gttatcaaag aagacttccc catgactcta aatgaatata
    24721 tcttacaata tcgcctgaaa caagctatag ataagatggc tgaatctccc aactcccctt
    24781 taagcgctat ctctgaccaa gttggatttt cagactataa atattttgcc aaagtattta
    24841 aaaagtatct ccatatttcc ccaaagaaat tgaaatcact cggaagaata gtcaaatagg
    24901 ctattcttct ttttgtagag aattttactc taatatagtg aattgaaata agatgtgaac
    24961 atctctatca ggaaagtcaa attaatttat agaaatattt tagcagtcaa agtgtactgt
    25021 tatagattca atacactata gaatagaatt gtaggtttta aaaacgctta cattgtttta
    25081 aaataaactt aaataacaca acggaggtat ccatcatgaa aaagaaatgg atgtattatg
    25141 ctgcttgttc ttctaatgaa tctgccgatg acagttcatc tgataaagga gacggtggtt
    25201 cgctagtcgt ttattcacca aactcagaag gcttaattgg agcaactatt cctgcctttg
    25261 aagaaaaata tggtatcaaa atagaactga ttcaagctgg tactggagaa cttttcaaaa
    25321 aactagagtc agaaaaagaa gttcctgtag ctgatgttat ctttggtggt tcttatacac
    25381 aatatgctac ccacggagaa ctctttgaaa actatatttc aaaagaaaat gataatgtta
    25441 tcaaagaata tcaaaacaca actggcttct ctactcctta tacactagat ggtagtgttt
    25501 taatcgtcca ccctgattta actaaaggca tgaacatcga aggatatagc gaccttctca
    25561 aacctgaact aaaaggaaaa atcgcaactg ctgacccagc aaactcttct agcgcctttg
    25621 ctcaattaac aaatatgcta caagctcaag gtggttacaa agatgataag gcttggtctt
    25681 atgtaaaaga tcttttcaca cttattgatg gtatagtaaa atgaaataag aacaggacaa
    25741 attaatcagg acagtcaaat cgatttctaa caatgtttta gaagtagagg tgtactattc
    25801 tagtttcaat acactataaa atcggttcaa gttcatctgg tgtctataaa gcagtcgttg
    25861 atggagaaat ggctgttggt ctctcttatg aggatccagc agttaaactc ttaaatgacg
    25921 gagctaacat taaagtagtc tatccaaaag aaggaaccgt cttcctacct gctagtgctg
    25981 ctatcgttaa aaaagctaaa aatatggaaa atgccaagaa atttatcgat tttattatct
    26041 ctcaagaagt acaagataca cttggtacaa ccactactaa ccgtcctgtt cgtaaaaatg
    26101 ctaaaacaag cgaaaacatg aaaccaattg acaaaatcaa aacactcact gaagattatg
    26161 attatgtcat caagaataaa tcagatatcg ttaagaaata caacgaagtc tttacagata
    26221 tccaatctaa acagtaaaag aggttcacta tgagtgagat cgaaattatt aacgccaaaa
    26281 aaatctacca cgatgtccct gttattgaga atttgaacat tacaattcca aaaggaagtc
    26341 tctttaccct tcttggagct tcaggatgtg ggaaaacgac ccttcttcgt atgattgcag
    26401 gtttcaacag tatcaaagat ggagaatttt acttcgatga tacaaaaatc aataatatgg
    26461 aacccagcaa acgcaatatc gggctggttt tccaaaacta cgctattttc ccacatttga
    26521 ctgtccgaga caacgttgct tttggtctta tgcaaaagaa ggttccaaaa gaagaattga
    26581 ttcaacagac caacaagtat cttgaactca tgcaaattgc tcaatatgcg gattgaaagc
    26641 ccgataaact cagtggtgga caacaacaac gtgtcacctt ggcatgcgcc ttagcggtta
    26701 atccaagtgt tctcctcatg gacgagccac ttagtaatct ggaggccaaa cttcgcttgg
    26761 atatgcgtca agccatccga gcaatccaac acgaagtggg aattacaact gtttatgtaa
    26821 cccacgacca agaagaagcc atggctattt cagaccaaat tgctgttatg aaagatgggg
    26881 tgatccaaca aatcggccga ccaaaagaac tctatcataa accagctaat gagtttgtgg
    26941 caacctttat cggacgcaca aatattatcc ctactaatct tgaaaaacgg agcgacggcg
    27001 cttatatcgt cttttcagat ggctatgccc ttcgaatgcc agctcttgat caggttgagg
    27061 agcaagctat tcatgtaagc attcgtcccg aagagtttat caaagatgaa tctggagata
    27121 ttgaaggaac tattagcgat agcgtctatc ttggactaaa tacggagtat ttcattgaga
    27181 caggttttgc ctcaaaaatt caagttagtg aagaatcaac ttttgaagaa gaactacaaa
    27241 aaggcaatcg tattcgtcta cgaatcaata cgcaaaaatt aaacatcttt tctgcagatg
    27301 gttcccaaaa cctgataaaa ggagtcaacc atggaatgta aaaaactaaa tatttggaca
    27361 acctcctctt tcttcatctt tcttacctat cttgtctttc tcgtttatcc tatcgttacc
    27421 gtgctcaagc aagcacttat acatgaagga caattctcac tagctaattt tgtcactttc
    27481 tttagtaaag tctactactc tgagacacta gtcaacagtt tcaaggtttc cattaccgct
    27541 actgtcactt ccttagttgt aggaacccta ttagcttatc tcttctctat gtatgacttc
    27601 aaggggaaga aatttctaca aatattgatt atcattgctt ccatgtcagc tcctttcgta
    27661 ggagcctact cctgggttct cttgctagga cgaaatgggg tcatcactaa atttttgaca
    27721 aatgcccttc atcttccagc tatcgatatt tatggattca aaggaattgt acttgtcttt
    27781 acactgcaac tattcccact ggtatttcta tatgttgctg gaacaatgaa cagtattgac
    27841 aattctctac ttgaagcagc tgaaagcatg gggtccttcg gatttaaacc tatcgtaacg
    27901 gttgttttac ctctcctagt tccaacctta ctagcagctc cttgcttgta tttatgagag
    27961 cattctcaga ctttggaacg cctatgttga ttggcgaagg atatcggact ttccctgtcc
    28021 tgatttatac ccaatttatt agcgaggttg gaggaaattc tgcttttgca attatggcga
    28081 ttatcattgc cttggcaatt ttccttatcc aaaaacacat tgcaaaccgc tacagtttca
    28141 gcatgaatct gctccatcca attgagccta aaaaaactac aaaaggaaaa atggctgcca
    28201 tttatgcaac agtctacgga attatcttta tctctgtttt acctcaaatc tacttaattt
    28261 atacctcttt cctaaaaaca tcaggtatgg tatttgttaa aggttattct ccaaacagtt
    28321 acaaggtagc tttcaatcgt atgggatctg ctattttcaa taccattcgt atccctttga
    28381 ttgccttagt tctagttgtt ctatttgcga catttatctc ctacctagcc gttagaaaac
    28441 ggaatttgtt tacaaactta attgacagcc tcagtatggt accttatatt gtatcaggaa
    28501 ccgttctagg gattgccttc atttcttcct tcaatactgg tctatttgga agtggatttc
    28561 ttatgattac agggactgct ttcatcttga ttatgtctct atctgtcaga agattacctt
    28621 atactattcg ctcatctgtt gctagcttac aacaaatagc accaagtatt gaagaagctg
    28681 ctgaaagctt aggaagtagt cgtctcaata cctttgctaa gattacaact ccaatgatgc
    28741 tatcgggtat tatttctgga gccatcctat cttgggtcac actgatttca gaactctcta
    28801 cttctatcct ctatcctcct ctacaatgtc aaaacaagaa caatgactgt agctatttat
    28861 acagaggttc tcagaggaaa ttacggtgta gccgcagcct tgtcaactat cctgactgtt
    28921 ctaacagtag gttccttgct cttgtttatg aaaatctcta aaagcaatag cattacactt
    28981 tagttttcca catcaaaaac agccgaaagg agtaccctcg gctgtttttt cttatcttga
    29041 tattcttatc aaatccctag ctcatggcaa gcaagtctag actgattaaa tagagccttc
    29101 ttccatcttc tcacgtccta gctctcggta actacggtca ccgacaactt caaccctaaa
    29161 ctgaccgtcc tcatattcaa gaatcgtcac gctaccatta tctagaccat gcggatgcat
    29221 gccattaatc agataaacaa tggttccaat agtcattcca tggctgacaa caagggcgtt
    29281 acctccacct tgatcttcca tttcttttgc aatcatttca aagccttcct tgattcggcc
    29341 actgagtttt tcccagcctt cagcccaacc agctgtatcg acctctacca agccctcagc
    29401 cagttcagca taagacaatt ggtgaacgtg gtccacatta aagatacgag gaataatgcc
    29461 catgaaaaga tcgccatcat aggctccatc aaaactaccg aaacaccatt ctctgatacg
    29521 cttgtccatg cgataaggga ttttcccctg caagccaagt tcttcaagga taattcccat
    29581 ggtctgaatg gtacgaccag aatcactcga ataagcgcgc tcaaactgta gatcagattc
    29641 tcgcaaaccg attcctaact cttgaatccc tcgttcacct tcagcagtta agggagtatc
    29701 gctccaacct tgcgcgcgac caatcgtgtt aaacatggtc ttgccatgac gtaccaaata
    29761 caatcgtact tttaccattt tccgtcctcc gtttgcttct attatatcat gaatgataaa
    29821 ataaaagcca cccatacagg cgacttttga aggagattat tatgaaaaag tttaggagtt
    29881 cattaaataa agatattaga tgaaaatcaa attcaaacta attcagtgtt atctgtttca
    29941 aataatatta ggaggtcttt atttaatgat tatagtatac acatctaatc ttaaatagaa
    30001 cttaaaattt ctcctatttt cttaattttt aaaactatga attggtgctg ggatttgtcc
    30061 accgcgagag atgaagtcga cagacgaagc cccattaacc ctcataacgg gtgcagttcc
    30121 taatagacca ccaaactcaa tcatatcgcc ttcttttcct ttgggaatga tacgaacagc
    30181 tgttgttttc atgttgataa caccgattgc tgcttcatcc gcaatcatag ccgcaatagt
    30241 ttcagcaggc gtatcttctg ggatggcaat catatccaat ccaacagaac agatagccgt
    30301 catagcttct agtttttcta aattaagaga gccattttgc actgcagcaa tcattccttc
    30361 atcctcagaa acagggataa aggcaccaga taaaccaccg acctggttac aggccatcac
    30421 tccacccttt ttaacttggt cgttcaagag ggccaaggca gccgtcgttc catgcgtgcc
    30481 aactgtttct agccccattt cctcaaggac acgtgccaca gagtctccaa ccgcaggggt
    30541 tggtgccaaa ctcaagtcca caataccaaa ctccacaccc agtctctcac tggccatttg
    30601 accaaccaat tgaccgatac gagtgatttt aaaggcagtt ttcttaactg tttcggctac
    30661 tacatcaaag ctctgtccac gaactttttc caaagcacgt ttcacaacac caggaccaga
    30721 aactccgaca ttgatgataa catctgcttc cccaacacca tgaaaggcac ccgccataaa
    30781 tggattgtcc tcaacagcat tagcgaatac aaccaacttg gccactccca tatctgaaag
    30841 atttgctgtt tccttgataa ttcgtcccat atctgccaca gccgtcatat taataccaga
    30901 cttggttgag ccgatattga ctgacgagca gaccttatcc gtctcagcca aagcgcgagg
    30961 aatggaattg atgagaatct catctccctt ttgataacct ttttgtacta aggcagaaaa
    31021 accaccaata aagtccacac caatctcttt cgcagcctta tcaagcgctt ttgccagaac
    31081 cacgtagtcc gtcgcatctg tcgctgcccc aatcagagaa ataggtgtca ccgatacacg
    31141 cttattaacg ataggaattc ccaactcagc cgcaatttca tcaccaacag ctactaaatt
    31201 agccgccttt gtcgtaattt tttgatagat tttctccgca gcacgattga tatctggatc
    31261 gatacagtcc aaaagagaaa tccccatggt aatggttctg atatcgaaat tttgctcctc
    31321 aatcatggcg atggtttcag taacttgtct aatatccatg ataacctcct agatattata
    31381 catagcttcg aaaatcgctg cactctgaat attgattttt acattcaaag tttgcccaaa
    31441 agcttcaaat tcattacgaa gataggtaaa atcttgcttt tcatcactag atacaatagc
    31501 catcatcgta aaatattcat ccaagacagt ttgagagata tcgtcaatat tcaatcctaa
    31561 ttctgcaatt ttaccagaaa cacctgcaac aattccagat ttatctttac caacaacagt
    31621 tataatcgct ttcataagca aactccaatt cttcttaaaa taagaaacct taacattaaa
    31681 caggattctt ttcaaatagt atagcataat tctaacgaaa atactcaaaa acgcctgctt
    31741 acgaagttgt tcttaagaaa aaaacaattt cgtaaacaag cgtctactat cccaacttat
    31801 gatgagtgtt cccgctagga ccgaaatcct agcggtagac cagagctaga ctaagagcac
    31861 aagactccat catcataaca ctcaacaaaa ttgatgattt tatactaatt cgatgatcgc
    31921 cattggcgct gcatcaccac gacgtggttc agttttaagg atacgagtgt atccaccgtt
    31981 acgttcagca taacgaggtg cgatttctga gaacaatttt tgaagtgctg tagtagaagt
    32041 gtacttatca gttgcttcat catagttttc agatgcgatt tcattacgta cgaaagcagc
    32101 tgcttgacga cgtgcatgca aatcaccacg tttacctaga gtaatcattt tttcaacagt
    32161 tttacggatt tctttagcac gagcttcagt tgtcacgatt gattcgttga tcaaaaggtc
    32221 agttgtcaaa tcgcgaagca ttgctttacg ttgtgagcta gtgcgtccta gtttacggta
    32281 agccatgtat tcctccttta tttatctttt aatccaagac ccaaatcaat gagtttgagt
    32341 ttcacttctt ccaaactctt gcgtccaaga tttcgtactt tcatcatctc tgcttcagat
    32401 ttttctgtca aatcatgcac agtattgata ccggcacgtt ttaaacagtt gtatgaacgc
    32461 acagacaagt ccagttcctc aatcgtacga tctaaaatac ggtcgtcaga ttcagtatca
    32521 gcttctttca tcacttcagt tgacttagca atctcagtaa gatttgtaaa caaatcaaga
    32581 tgttctgtca aaatacgtgc tgaaagccct aaagcatctt ctggaataat tgttccattt
    32641 gtcaagattt caagggttaa tttgtcgaaa ccatcattgc tacctacacg agcaggttcc
    32701 acttgatagt tgacttttgt aactggtgta taaatagaat ctacagcaag tgttccaact
    32761 ggtgcattat cctttttatt ttcatcagca ggtacatatc cacgaccact gttaacagtc
    32821 atagtcgctt ttagagaaga accttcaccg attgtaaaga gataatgatc tggatttaca
    32881 atttcaatat cgctatctgt caaaatgtca ccagctgtta cttcagcagg accttcaaca
    32941 tccagttcga tgattttttc gtcttcaacg tacgatttca ctgcaattcc tttaatgttc
    33001 agaatgattt gcatcacgtc ttcacgaaca cctggaactg tgtcaaactc atgtaacaca
    33061 ccatcaatgt tgatagatgt cacagctgct cctggtagag aagctagaag tacacgacga
    33121 agagagttac caagagttgt accgtagcca cgttcaagtg gttcgattac aaacttgcca
    33181 taatctttat tttcatcaat ttttgttata tttggttttt caaactcgat catttagtta
    33241 ctccctctta aacgaaaagc agtgtaatgc gatgattata cacggcgacg ttttggagga
    33301 cgagcaccat tgtgtggcac tggagtcaca tcacgaattg ctgttacttc aagaccagcg
    33361 gcagcaagcg cacgaatagc tgactcacga ccagaacctg gaccttttac agtaacttca
    33421 actgatttaa gaccgtgttc ttgtgcagat ttagcagcag cttcagaagc catttgagca
    33481 gcgaatggtg tagatttacg agaacctttg aaaccaagag caccagctga tgaccaagca
    33541 attgcattac catgcacatc agtaatcata acaatagtgt tattaaatgt agcgtgaata
    33601 tgagcaatac cagattcgat attctttttc acacgacgtt tacgtgttgg tttagccaag
    33661 acttttacct cctatattat tttttcttac cagcaatcgc aacagcttta cctttacgag
    33721 tgcgagcgtt gtttttagtg ttttgtccac ggacaggaag tccacgacgg tgacggatac
    33781 cacggtatga accgatttcc atcaaacgtt tgatgttcaa gtttacttca cgacgaaggt
    33841 caccttcaac tttgattgca tccacttcac gacggatagc atcttcttga tctgatgtaa
    33901 gatcacgtac acgaacatct tctgagattc cagcagcagc caaaattttc ttagatgttg
    33961 caagtccgat accataaaca taagtcaatg agattactac gcgtttgtca tttggaatat
    34021 caactccagc aatacgagcc atgttttctc ctttctatct tatccttgac gttgtttgtg
    34081 ttttggattt gctgggcaaa ttaccataac acgaccatta cgacgaataa ctttacagta
    34141 ttcgcaaatt ggtttgaccg atggtcttac tttcatttct tatccctcca agtttttcga
    34201 ttatttaaag cggtaagtga tacgtccacg tgtcaagtca tatggactca tttcgacagt
    34261 aacacgatct cccgctaaaa tacgaatata gtttttacga attttaccag aaactgttgc
    34321 taaaatctga tgtccatttt caagttcaac cgtaaacatt gcattcggca ttgtatcaac
    34381 tactttgcct tcaacttcaa tcacatcgtc ttttgccacg caaaagcacc tccataaatt
    34441 tcgattcgat gcctctagac acagagacaa caattataag tcagactatc tcagtataac
    34501 atttgtagcg gatttttgca agtgcgaaaa acgctttatt tcaaatttgt caatactttt
    34561 tcaatatctg agaagacatc attgatatct tgattacctt cgatgtcatg aaccaaacct
    34621 ttggcacggt agtgagcaat gattggttct ccttgagcaa tattaacatc caaacgacgt
    34681 tttactgtct caggcttatc atcttcacgt tggtagtaat cttcttcttt atagtcaact
    34741 ggtgggttaa agaccttgtg gaaagtttct ccagttacgc ggtggatgat acggccactc
    34801 aaacgttcca agaggctgtc agggttcact tcaatattga taataccttc tagttcaatg
    34861 ccaagttcag ccaatgtttt gtccaaggca tgagcttgtt caattgtacg tgggtaacca
    34921 tccaataaga atcctgtttc tttaatatca tcttgtgaaa ggcgttcttt tacgattcca
    34981 tttgtaactt cgtcaggaac caattcaccc ttgtcaatat atgacttagc aagaacaccc
    35041 atttcagttt gatttgccat tgcagcgcgg aacatatcac ctgttgagat atgtgcaaca
    35101 tggaattgtt ctacgatttt tgctgcttga gttcccttac ctgcaccagg taagcccata
    35161 atcaaaagat tcatgatttg atctccttat tttattttta aatagaagca aaaagtcttt
    35221 tcacccctat ttaaaaacaa aatagaagag gggagatcca actcttacaa atgtcagagt
    35281 ttaaacctcc actccctcag tatttactga ttcagtaaat acttttattc tgttctgtcc
    35341 atgaaaccaa catacttacg tttcaatagg taaccttcca attgcttgat tccttcgata
    35401 cctgtagaga taatgatcaa gagacttgtt ccaccaaagg caacaacatc agaaagacca
    35461 aatacatctt tagctgcaat cggtaaaatg gaaatcacac caaggaagag ggaaccaaca
    35521 gttgcaagac gacgaagaag tttagacata tattcttctg tacctttacc aggacgaact
    35581 ccatggatat aggcaccact cttttgtagg ctctctgctg ctttttcagg attaatctgt
    35641 acaaacgtat agaagaatgt aaagagaata atcaacaaag catacatggc aataccagtt
    35701 ggagaagtag ttgccaacat ctcttgtgct acccttaccc aagcccaatc atgacctgtg
    35761 gcactcaaaa actgaagaat agccgcaggc gctgcagtaa tcgaactggc aaagataaca
    35821 gggataactc cagcagggtt tacctttaac ggaaggtaag agctagatgg agcaccttgt
    35881 gcaaccttag tatattggat tggaattttg tattctgctt gttgaacata agttgtaaag
    35941 taaataatca acaatacagt aataatcaaa atgattacga aaatgataga tgaagtgata
    36001 cggctacttg ggacgttcac aaagtagtcc acatagatgc cctgaatcat ctctggaatt
    36061 gaggaaacaa tcccggcaaa gataatcatg gaaacaccgt ttccgtatcc cttatctgta
    36121 atttgctcac ccaaccaagt gacaatcata ctaccagctg ttaagatgat accaatcgtc
    36181 agaaaaactt gtggagttaa agcagtttta atcaattgag ctccagctaa ggtattaaaa
    36241 ccagctgtaa tcccgataga ttgcacaaaa gcgagaacta gagcaatata acgagtagct
    36301 tgattcaatt ttcttcgacc tacttcccct tgtttacccc actctacaaa cttgggtaaa
    36361 atatccattt gcaagagttg gacaacaata gaagcggtga tatagggact aactcctagg
    36421 gcaaaaatcg aaaagttttt tagggcattc cccgacacca agctcaacat gtttaagaag
    36481 gataatccac ttaaagcatt caagctattg gcattcacac caggaactgt aatgctagtt
    36541 ccgatacgaa agaccaaaac gataaaaatt gtaaataaaa tttttgatcg aacctgcttg
    36601 actttaagag cttctcttaa taatttaaaa aacataggtc acctctctta gatgacttct
    36661 actgaaccac ctttagcagt gatagcttct tcagctgatt tagagaattt agctgctttc
    36721 acagtcaatt tcttagtcaa ctcaccgtta ccaagaattt taatacctga cttttcagct
    36781 ttaacaattc ctgcttcgat aagaacaact ggagttactt cagcaccatc ttcaaagacg
    36841 ttcaattggt caaggttcac aattgcgtat tctttagcgt tgatgttagt gaatccacgt
    36901 tttggaagac gacggaacaa tggagtttgt ccaccttcaa aaccaaggcg aactccgcca
    36961 ccgctacgag ctttttgacc tttttgacca cgaccagatg ttttaccgtt acctgatgaa
    37021 gtaccacgac caacgcggtt acgtacttta cgagaacctt ctgcaggttt caattcatga
    37081 agtttcattt ttattttctc ctcttttgta aaatgctagc gccgataagg gagaaaaggt
    37141 tgtctccccc atcaactcgc ctatacgatg tcatcataga tgactatatc tagttttagg
    37201 ggatggtaca gtgcacatcc cctaaaactt cattagttta cttcttcaac tgttactaag
    37261 tgagatactg ctgtaatcat accacggata gcagcgttat cttctttaat aacagagctg
    37321 ttcaatttgc caagtccaag tgctacaaca gttttacgtt gtgatggaat gcgtccgatt
    37381 ggagacttag tcaaagtaat tttaatttga gccattttat cccctttctt atgccaaatc
    37441 agaaactgaa ataccacgaa gggcagcaat ttcttcagcg cgtttcaatt gtttcaaacc
    37501 ttcaacagtt gcacgaacaa tgttgattgg agtgttagaa ccaagtgatt tagatgtaat
    37561 atctgccaca cctgccaatt ccacaacggc acgaactgca ccaccagcgg caactccaga
    37621 accttctaca gcaggtttca acaatacttt agctccaccg aattctgaaa gaacttcgtg
    37681 tgggattgtt gttccaacca taggaacttc gatcaagttt ttcttagcat catctactgc
    37741 tttacggatt gcttctggaa cttcttgagc tttaccagta ccaaatccta cgcgaccatt
    37801 gtggtcacca acaacaacaa gagctgcgaa acgaagacga cgtccacctt taacaacttt
    37861 tgtaacacgg ttgacagcaa ctacgcgttc ttctaattca actgcattgt ctttaaatgc
    37921 cattttctag tgtcctccta ttagaatttc aatccgtttt cacgagctgc atcagccaaa
    37981 gctttcacac gtccgtgata tagatatcca ccgcggtcga acaccacttc tgaaatacct
    38041 ttagcgtttg cacgttctgc aacgagttta ccgacagcaa cggcttgttc agttttagtt
    38101 ccttttgaaa cttctttatc aagagttgaa gcacttgcga gcgttacacc cgctacgtca
    38161 tcaatcactt gagcgtagat gcctgtatta gaacggaata cgttcaaacg tgggcgatca
    38221 gcagttccag agagttttcc gcgaacgcga cggtggcgtt tttggcggag tttgttttta
    38281 tctggttttg aaatcacagt tttcacctct ttagttttaa atcgtgtgct atgcacaaag
    38341 ttggaaaata ggttggtggt tgatgatcaa ccactcaaca ttatttacct gttttacctt
    38401 ctttacggcg aacgaattca ccaacgtaac ggataccttt acctttatat ggttctggtg
    38461 aacgaaggct acgtacgtaa gcagctgttt gaccaactac ttcttttgaa attccgctaa
    38521 caacgattgt tgttgggttt ggaagttcaa aagtaattcc ttctggagct tcaacttcgt
    38581 ctggatgaga tttaccaaca gccaaaacaa gtttagatcc ttgaagctgt gcacggtaac
    38641 caaccccacg catttcaagt tctttcttga atccttctga tacaccaaca accatgttgt
    38701 tcaaaagggc acgagtagtt ccgtggatag ttttcatttc ttttgaatcg tttggacggt
    38761 gaagagttat ttcagtacct tccacacgga tttcaatatc ttttgagaac tcacgagtaa
    38821 gctctccttt aggtcctttt acagttacaa cgttgtcatt gttagcgagt tcaacaccag
    38881 caggcaacac gataacttta ttaccaatac gtgacatgtt tattttctcc tgttaaattg
    38941 tcaggccaga acggccagtt ttcacggggt tcagatactt atttagttca agagctgaac
    39001 tgaacacgct ctaagaactg tgcaaaatag ataagttggc ttgtttgtaa acaaacttct
    39061 ccaacttcct aattttgctt tgttctttac gagctttgta tcttgatttt accaaacgta
    39121 agcgataacc tcaccaccaa cattcttttg gcgtgcttct ttatcagtaa gcaaaccttc
    39181 agaagttgaa aggatggcaa ttccaagtcc gttaagaact tttggaaggt cttcacgttt
    39241 tttgtagaca cgaagtcctg gtttagaaac acgtttcaag ttagtgataa ctttctcacc
    39301 atttggtccg tatttaagaa atacacggat gacgccttgt ttgtcatctt caatgatttc
    39361 aacgtttttt acaaaacctt cgcgtttaag gatttcagca atcccttttt tgatgtttga
    39421 tgcaggtact tcaagtactt cgtgtttagc ttggttagca ttacgaatac gagttaggaa
    39481 gtctgcgatt gggtcagtca taaccatttt gtttttctcc tcttactagt agtttgcaag
    39541 ttgcacttgc tagttaatac atgatacaaa gagcgtaaca gcacgagcaa aaatagacaa
    39601 tttaatgcag aagcaatgct ccaaagcaaa ttggtctttt ttgccaaagc tgtagctcgt
    39661 gttcaaattg gcaagcccaa ttgaacccgg gctaaactct gtgtgaaaaa gataaacttt
    39721 cctagaaact ttagtttctt cgtcaagttt cctattttta cttggagttt tgacactctt
    39781 gatatcttaa attaccaaga tgcttttgtt acaccaggaa tttgaccttt atgcgcaagt
    39841 tcgcgaaaag cgatacgact cagaccaaat ttgcggtaaa ctgaatgtgg gcgccccgta
    39901 actctacaac gattatgtaa acgagtcggt gaggcgttgc gaggtaattt agataaacct
    39961 tcgtagtccc ctgccgcctt taatgcagca cgtttttcag cataacggtc aacaattttt
    40021 tggcgtttag cctctctagc taccattgat tttttagcca ttagatttac ctcctatatt
    40081 attttgcaaa aggcattcca aggcctgtaa gcaatgcacg tgactcttcg tcagtgttag
    40141 cagttgttac gataacgatg tcaagaccac gagttttgtc aacgtcatcg aagttgattt
    40201 ctgggaagat taattgttct ttcacaccaa gtgtgtagtt cccgcgtcca tcaaatgatt
    40261 ttgttgggac accgtggaag tcacgtacac gcggaagtga aactgatacc aatttatcca
    40321 agaattcgta catacgttca ccacgaaggg taacttttgc accgatcgca acaccttcac
    40381 gaagacggaa gccggcgatt gattttttag ctttagtgat aagtggtttt tgacctgaga
    40441 taagtgccaa ttcttcagca gctttttcaa ggcttttagc gtttgataca gcttcaccaa
    40501 cacccatgtt caaaacaatc ttatctactt taggcacagc catcactgat gagtagttga
    40561 attgttctgt caaagcagga actacttcat taagatattt ttcttttaaa cgatttgcca
    40621 ttatacttct cctttccttc gtgattaatc aagcacttcg cctgattttt tgttgtagcg
    40681 aactttttta ccgtctacaa atttgtatcc aacacgacca gctacaccat ttttgtccaa
    40741 aacttgaacg tttgatacgt ggatagctgc ttctttctcg atgataccac cttgaggaag
    40801 ctcgttagtt ggacgttggt gtttcttaac aatgttaaca ccttcaacga taactttgtt
    40861 tacttttgga agggcagtaa ggacaacagc ttctgttccc ttatctttac cagcgattac
    40921 gcgaactttg tcgccttttt ttacaaacat taggtttctc cttgattttt cttacgccca
    40981 taagggcacc ctagcttgaa gctaggggac tagtttgttc ctaaaaatta aagtacttct
    41041 ggagcaagtg acacgatctt catgaagcca ccttcacgca attcacgtgc aactgggcca
    41101 aagatacgtg ttccgcgagg agttttgtct tcacggatga taactgctgc gttttcgtca
    41161 aatttgatgt atgaaccatc agcacgacga gcacctgatt tagtacgaac gataactgct
    41221 ttaacaacgt cacctttttt aaccgcacca ccaggagtag cttgttttac agatgccacg
    41281 ataacatcac cgatgtttgc aaatttacgt cctgaaccac caagaacttt gatagtcaag
    41341 atttcgcgag caccgctgtt gtctgcgact ttcaaacgag tttctgtttg aatcatttca
    41401 gttttctcct ttcaggtttg attagatgat gaccgcttct tcaacaactt ctacaagacg
    41461 gaaacgtttt gtagctgaaa gcgggcgagt ttccatgata cgtacgatat cgccttcttt
    41521 ggcaacattg ttttcatcat gagctttgta ttttttagag tagttaatac gtttaccata
    41581 gactgggtgg ttacgttttg tttcaactac aactgtgatt gtcttgtcca ttttgtcaga
    41641 tacaacacgt ccaacaagaa ctttacgatt attgcgttcc attgaaattt ctccttccct
    41701 agtctattat ttcgcttcag attgaactgt tttgatacga gcgatttgtt ttttaacttc
    41761 tttcaagcga gctgtttgtt ccaattgacc agtagcagct tggaaacgaa gttcaaacaa
    41821 ttcttttttc aattcgtttt cgcgcttcgc gagttcttct tgagaaagac cacgaagttc
    41881 tttaacaaat tcttttactt cattaagttt catgccttct ccttattctg cttcacgttt
    41941 tacgaattta catttaactg gcaatttgtg gctagcaagt cgaagcgctt cacgtgcaat
    42001 ctcttcagat acaccagcga tttcgaacat cactttacca cgtttaactg gtgctaccca
    42061 accttcaggt gcccctttac cagatcccat acgcacaccg atagctttag cagtgtatga
    42121 tttgtgtggg aagattttaa tccaaacttt accaccacgt ttcatgtaac gagtcatggc
    42181 gatacgagca gcttcgattt ggcggttagt gatccagtgg ctagttgtag cttgaagacc
    42241 gtattcaccg aatgctactt cttttccacc ttttgcttca ccgcgcattt ttccacggaa
    42301 ctcacgacgg tgtttaacac gtttaggtac taacattggt tatttacctc ctttagtgtt
    42361 tttacgagct ggaagaactt caccacggta gatccatact ttaacaccaa gtttaccgta
    42421 tgtagtatct gcttcttccc aagcgtaatc gatatctgca cgaagtgtgt gaagcggaac
    42481 agttccttca gagtatcctt cagcacgggc gatatctgca ccgttcaaac gacctgatac
    42541 ttgagttttg attcctttag ctccagcacg cattgcacgt tggattgctt gtttttgtgc
    42601 acgacggaaa gcaacacgtt gctccaattg acgagcaatt ccttcaccta caaggtgagc
    42661 atccaaatca ggttgtttga tttcgatgat gttgatgtgt acttgttttc cagtcaattt
    42721 gttaagtttt gcacggagtg catcaacgtt agcaccacct ttaccgataa ccatacctgg
    42781 tttagcagtg tgaagtgaaa cgttaacttt gtttactgcg cgttcgattt caatagttga
    42841 aactgctgcg tcagcaagtt ctttttgaac gaatttacgg attgcaagat cttcatgaag
    42901 gtaatccgcg tattcttttt cagcatacca tttggcatcc caatcacgga tgatgccgac
    42961 acgcatacca attggatgta ctttttgacc cacgatttta cctccttatt tttctgcaac
    43021 agctacagtg atgtgagctg tacgtttgtt gattggtgaa gctgaacctt tcgcacgtgg
    43081 acggaaacgt ttcatagttg gtccttcgtt tgcgaatgct tcagatacta ccaagttagc
    43141 tttatccaaa ccaaagttgt tttcagcgtt agctacagct gagttcaaaa ctttcaagat
    43201 gatttcagca gctttgtttg gagtgaatgt caagattgcg attgcatcgg ctacgctttt
    43261 accacggatg ttatcaagaa caagacgtga tttacgaggt gaaacacgta ctgtacgagc
    43321 cattgcttta gctgaagtaa tttctgccat ttatgttctc cttattttct acgtgttttc
    43381 ttgtcgtctg cagcgtgacc tttgtaagta cgagttggtg caaattcacc aagtttgtgg
    43441 cctaccatgt cttcttggat gtaaacaggt acgtgtttac gtccgtcata aactgcaata
    43501 gtgtaaccaa tgaaacttgg gaagatcgtt gatcgacgtg accaagtttt aataactttt
    43561 ttcttttcgt cgttagcttg agcttcaact tttttcatca aatgctcatc gacgaaaggt
    43621 ccttttttaa gactgcgtcc catttttata ttttctcctt taaatgttgt accacagcgg
    43681 cttgcactcc tatggagcgc taccgagctg gcggatttac tagttgctta agcgactagt
    43741 ttaatattat ttctcgttgc gacgacgaac gataagtttg tcagatttcg ctttcttgtt
    43801 acgagtttta agaccaagag caggtttgcc ccatggagta gatggtgctt tacgaccaac
    43861 tggtgcctta ccttcaccac caccgtgtgg gtgatcgtta gggttcatta cagaaccacg
    43921 aactgttggg cggatacctt tccaacggct acgtcctgct ttaccaaggt ttacaagtcc
    43981 atgttgttcg tttccgacaa caccaactgt agcacggcaa gttccaagaa tcatacgaac
    44041 ttcacctgat tgaagacgaa caagaacgta tttaccttca gaacccaata cttgagcaga
    44101 tgcaccagca gcacgtacca attcaccacc acgacctggt ttcaactcga tgttgtgaat
    44161 caaagtacca actgggatgt tagcaagtgg aagagcgttt ccgactttga tatctgcttc
    44221 tggacctgaa acgatacgtt gacctacttc aagacctttt ggagcgatga tgtatgcttt
    44281 cacaccgtca gtgtagtgta caagagcgat gtttgcagaa cggtttggat cgtactcgat
    44341 tgttttaaca actgcttcaa cgttgtcttt attacgtttg aagtcaacca aacggtagaa
    44401 acgtttgtgt ccaccacctt ggtgacgaac tgtgatacga ccgttgttgt tacgaccagc
    44461 cttgctcttc aatgcaacaa gcaatgattt ttcaggagtg cttgttgtga tttcagcgaa
    44521 atccaaagaa gtcatattac ggcgaccgtt tgttgttggt ttataaacac gaattcccac
    44581 gatatttcct ccttagatta ttcagcttca gcagcaaaca actcgattgc tttagaatca
    44641 gctgtaagtg tgatgatagc ttttttagtt ttgttagtaa aaccagtgta acgtccaaca
    44701 cgtttagctt ttggttttac gttgattgtg ttaacattgg caactttaac accttcgaaa
    44761 gcagcttcaa cagcttgctt gatcaaaagt ttgtgtgcac gagtgtcaac ttcaaataca
    44821 tattttcctg cttcaagttg agccattgag ctttcagtga tgacaggttt tttgataaca
    44881 tcatacaaat tcattatgca agaacctcct cgattttaga gatagctgct tgtgtgacaa
    44941 gaagtttgtc gctatttgcg atgtcaagaa cacttgcagt tgtagcagtt gcaactttca
    45001 cgtttggaag gttacgagct gaaagagctg cgaattcatt tccttcttca aggataacaa
    45061 gaactttaga atcgatgctc aatgctgcaa gaacttttgc aaattcagca gtttttggag
    45121 ctgtaaatga aagagcgtct acagctacga atttgttttc agcaactttt tcagagtaaa
    45181 ctgatttaag agctaggcga cgaacttttt gtggaagttt gtagccgtat gaacgtggag
    45241 ttggtccgaa gacaacacca ccaccacgcc attgtggtga gcggatagaa ccttgacgag
    45301 cacgtccagt tcctttttga cgccatggtt tgcgtccacc acctgatact gcagagcggt
    45361 ttttaacagc gtgtgttcct tgacgaaggc ttgcgcgttg gctgatgatt acatcaaaca
    45421 caactgattc atttggttcg ataccaaata ctgcatcgtt aagaacaact tggccagctt
    45481 ctttaccagt ttggtcaaat aatgttacgt ttgccattgt gactgatttc ccctttcttt
    45541 attatttacc agctttaact gctgatttga tagtgataag agatttctta gcacctggta
    45601 cgttaccttt gataaggata acgttctttt ctggaacaac ttgtacaact tcaaggtttt
    45661 gaattgttac gcggtcgcca cccatacgtc ctgcaaggtt tttacctttg aatacgcggt
    45721 taggtgcaac aggccccata gaacctggac gacggtggta acgagaaccg tgagccattg
    45781 gtccacgtga ttgtccgtgg cgtttgataa caccttggaa acctttacct ttagaagtac
    45841 cagttacatc aacaacatct ccagctgcga atgtttcaac tgtgatttca gcgccaactt
    45901 ccaagccttc aacgtttttg aattcacgaa tgaagcgctt aggagccgtg ttagctttcg
    45961 ctacatgtcc tttagcaggt ttgttgctca atacttcgcg tttgtcatcg aaaccaactt
    46021 ggatagcgtt gtatccgtct gtttcaacag ttttaacttg aagaacaacg tttggagttg
    46081 cttcaataac tgttacaggg atcaattcgc cagcttcagt gaagatttga gtcattccca
    46141 cttttttccc taagattcct tttgtcatga gaaaatagtt ccttttctat attttttatt
    46201 caaaaagttt ttaacgagcg ttttttatgc tcaagtctaa gatacaaagg gcgtcaaact
    46261 caaagtgaaa ataggaaact aacgcagtgt ctagcggaca ataggaagtt tatctttttc
    46321 acaccgagtt tagcccgtgt tcaattagat tgaaacacca gcctctgctc ttttatattt
    46381 tagattaaag tttgatttct acgtttacac cacttggaag atccaatttc atcaaggcat
    46441 caactgtttt ttgagttggg ttaacgatat cgatcaaacg tttgtgtgta cgcatttcaa
    46501 attgttcgcg agagtctttg tatttgtgag tcgcacgaat gattgtgtag aggctacgtt
    46561 cagttggaag tgggattgga cccgcaactt gtgcacctgt acgagtagct gattctacga
    46621 tttttgcagc cgctgtgtca agcgtacggt gttcgtaagc tttcaaacgg atacggattt
    46681 ttttgtttgc catctttttc tccttttcgt ctatttaaga taataggcta gctccacaag
    46741 aaaaccgacg cggtgttgcg tggcaatgca accgagcgtg tcgcaacctc ttgcatcaaa
    46801 actaaggctg taatttacag caccataata gaataacaca aatcccctgc gattgcaagg
    46861 gatttgttag gttttttcgt ttttttggga tgaaactgtt ttcctacttt ttctttctaa
    46921 aattagggat tttcttctat atttcttcta tacataagtt aagtcctctt gacatcaaat
    46981 ttatcttctg attggtccac taagatatcc gccttagact cagtttcttt atagtatcgc
    47041 agatactgct cccgtctcat ctgatggcta gccaatataa aggatgcatc gcgatttctc
    47101 acagtcgtat ctctagcaag gcgtcgcttt aattcggtct cctcatccgt gtagaaacag
    47161 atggtttttt caaagagttc cttgggtaga aaaccaacag acatcccttc gacaatcaaa
    47221 attggtttgg ctccagacaa gacctcacta gccttccaag gttcttcaat tgtcaagaca
    47281 tccatacccg cctgcaaggc aaggatatct ctctgcaaac tctccagttc atgcgccact
    47341 ggcagactgg ctgtcacctt ttgattcggc gcgtccttgg gtactaccaa atagcgtccg
    47401 gaggtgatat agggatctgt ttctagcaaa tttactgtag tagaatctaa agcttggtac
    47461 aattcctgtg caaaggttga tttacctgaa gctccatgac cgtatattcc cagtgtcctg
    47521 actttccccg tctcgatctc tgagactagt tggtctacta agtctttttt cttcattctc
    47581 tcttcacctg ttcatagctt tcttttccgt cattgagctt gtcccaaatc actacttgcc
    47641 cacttttgag cgatttttgc acatcgataa ttcgttggtt ggacgaacct cgaaactgga
    47701 gcatgagatt tctcttagtt cgatcgtatc gtccatcgac aagaatgtca atcaatgata
    47761 agagttccag tttatctgga gtttccaaca tcatttcttc ccaagtgtag cccgtccagg
    47821 accaaatgtc cttgtctggc aattcctttc ggatgcgttt aactagaggc aagagaatgc
    47881 cagtattgag aaaaggctcc cctcccagca aagtcaagcc ttgaacatag ggttgggcaa
    47941 ggtctgccat gatctgctct tctaattctg ctgtataggg aatgccagca ttaaaagacc
    48001 aagtcgcaac attataacat ccctcgcagt gaaacataca gcctgataca tagagagagt
    48061 tacgcacgcc ttcgccatca acaaagttaa aggccttgta gtcaatgata cgaccttgac
    48121 taagttcctc gcttttccat tcttgtggtt ttggattatt cattcgctac ctctatccaa
    48181 taacgctcga ctccattgcg agcatcctca aatattccac catttgctag aatgactgct
    48241 ctgctagcag gattattcac gctacaggtc accagagctt tcttgatgtt cttttcctta
    48301 gcaacttgca agccctgacg gagagtctct tttgcataac ccttgcctct ttcagatgga
    48361 cggatggagt agccaatgtg cccaccttct tctagtaaaa agttactgag gcgcaaccgg
    48421 agattaagaa atccaactgc ttgacctttc tcagaaaaag ccactaactg aattgcagga
    48481 acccatcctt caggcagatt aatccccatt tcctgttcct gattgctttc taaccagtct
    48541 tcatacacaa agttctctgt atcccagaaa ccgccgtcgt gaggcgactg aaatttttca
    48601 aactctgtca tcatatctaa aacagctttc ttatccgcta atcttggtct gcgtaattcc
    48661 atccttacct cccactatga gaaaagcgag agctggctct cacttttatt ttttcttgtt
    48721 cttgtttaaa aattgttctc tgaagctaag caagcgttct ttcttaccag ctccaccttt
    48781 agagtgtttc tcgtgataac gggtcacttg tgcgcgcccc ttatcgtcta gttgatattt
    48841 tcccatttca tttctttcta atttgttact tgatgcccag ctattttaat cgttgaacca
    48901 ttcatatgtt tgacacgcgc agcgatttcc ttgtgacgcc cgttgaccat cggtcttgct
    48961 tgaggattac ctaggtagcc acaagttcgc ttcaccacat ctactgtttt agggtcgcta
    49021 ttgccacagt ttggacaagc aaaccctctc tcagttggtt caaaatcccc ttcaaagtca
    49081 cacttgtagc aacggtcaat cggagtattg gtgcctagat agcctacacg gtcataagca
    49141 taatcccaga cagcttccaa ggcctttgga ttttgctgaa ggactggata ctcacaataa
    49201 tggatgaaac cacctgacgc acctgcttct ggatagactt tctcaaagtc caatttttca
    49261 aacggtgttg gatttttacg aacatcgtag tggaaagagt tggtgtagta ttccttgtct
    49321 gtgatatcag gaatagagcc aaacttgtct atatctagtc ggcagaaacg gtctgtcaga
    49381 ctttcggatg gtgttgagta gatagagaaa tggtagccat attggtctga ccactcttct
    49441 acacggcgtt tcatatcgtg aatgatgtct agcgtgaatt ccttagcatc tggattactt
    49501 tcccagctgt taccaaagaa aactgtcgct acttcataca agccgatata gcccagcgaa
    49561 acggtcgcac gacgattctt aaagagctgg tcaacacttt cttctttacc tagacgatgg
    49621 ccaaaagcac cgtactgata aagaatagga gcattcgctg gtgtcgcctc tttagtgcgt
    49681 tcgacacggt aaacaagagc atcttctgcg atattcattc gctcgttgaa gatttcccag
    49741 aacttattca tatcaccttc agactcaaga gcaatacgag gcagattaac cgtcacaaca
    49801 cccagattca tgcgacctga attgacttct acaccatttt catccttcca cccttgaagg
    49861 aaagaacggc agcccatagg caccttgaaa gaacctgtca aatcaacaat cttatcataa
    49921 gacaagacgt ctggatacat ccgcttggtt gcacactcta gagccaactg cttgatgtca
    49981 tagttgggag ttccttcctc taagttgagg cctcttttaa gcgtaaagat aagtttagga
    50041 aagatagccg tacggtgttc tgaaccaaga cccttgatgc gaatgtttaa aatagctttt
    50101 tgaatttctc gttcaaaacg actggttccc agaccaaaac ctaacgaagt aaaaggtgtt
    50161 tgtccatttg aagtgaagag agtattgatt tcatactcaa gagattgcat ggcatcgtag
    50221 atgtcctttt gcgctttctt ccaagcgtaa tcttcctgtt tttcaggcaa tacccactct
    50281 tctgcatctt tgagatgttt ttgataattc ttctctgcat aaggcgccaa aatttcatcg
    50341 atacggtcag ctgaacagcc accgtactgg ctagaagcaa cgttggcaat gatttgagaa
    50401 atctgtgctg tcgcagtctg gatagacttg ggactctcta cctctgcatt tccaatctta
    50461 aaaccatttt ccaacatacc cttaaaatca atcaaacagc agttggtcat aggggtatag
    50521 ggactgtagt ccaaatcgtg atagtggata tccccctttt ggtgggcatt ggctacgtgc
    50581 ttaggaagca tttgcagtcc gattgatttc ccaacaatcc ctgctgtcaa atcacgctga
    50641 gtgttaaaga catcactgtc tttattagca ttttcattga caactgtctg gtctttgttg
    50701 agaagtttat gaatactaaa gttgatatcc gtcgcttttg agcgctcaaa atccctctgt
    50761 gtccgataag taatatactc ctcagccagt gcatattctt tggcttcaag gagttcatgt
    50821 tctacgatat tttgaatttc gtaaatctta attccctgtg gaaagcgact atgaatttct
    50881 gtaataattc gctcagtcag atcattgagg cgtttttcaa ccaagggtgt cacatccata
    50941 accttgtcag ccgccttgtg gagagccttg tcaatcttgt ccacatcaaa tacaacacgt
    51001 ctcccatctc gtttctcgac gaagagagtt ggcaaaattg taattttttc ttctagtgca
    51061 atcatatgca tgctcttttc taaaatgttc tttctaaaaa gtattatacc actctaaaaa
    51121 gaaaaatcaa tatcttgtgt taaaaatcct aaaattttta aaaatacaca agatattgat
    51181 ttggttattt aattttatag actttaaact ctgagtaatc agtctttact tgttggtaat
    51241 tttcttttaa gagtgtttcc acttcagacc agactaccac cttgtcattt accacaatca
    51301 ccttaggttg tttttctttc aagtcattaa gtaacttatt acgattttct tcagttgctg
    51361 tatagtgcaa cggggacaaa atcgccgatg gcgacaagcg ctcactctta cgataaagag
    51421 tcgcagtatc atcccatgca tagatagcat cttccgtatt tgtctcctct tttaccaaat
    51481 cagcaagacg attacgttct tgataaggta ctggataaag gacaaacttc atcaaaaaag
    51541 gaacagacaa aagatagaca aacccaaaaa ctagcagata tagatttcct ttgaagaaac
    51601 gactccatag gctaactggc gtttctcttc ggcttcttct agcactacgc cccctctgtt
    51661 gactcttaat attggttacc aataaaagta ggaaaacagg gaagacgacc atcaaataag
    51721 aagcgtgcag aggctcttgt gaaaagaaaa gtaaaatcaa acctaaaatt accacaagac
    51781 ttgcaggaac cgaaatgacg tatagtttaa agggtttgga ttgaaagaga cctaaaaaga
    51841 tacaaaccac aaatcccaaa ccaagggaca acaagccata aaaaaacata ttctctaaaa
    51901 ttttagaagt aaaatcaaag cgaatactgt caataggata acgaatacca ctaatcgcat
    51961 ccccaaaact tcctgttgca gcactatagt aggcagttgg ataaaagaca agtgaaaaac
    52021 ctaaagccac tgcaagaaac tgataaaacc catgcacaaa acgtctatgc ccaaggttaa
    52081 agaccaacaa gcctaaactc actacagcaa taaacaggag cgatgataag ggagcaaaga
    52141 aaaatccgcc tgccaaagct agcccaatcc gtacaaatcc cttatcatgg cttggattgc
    52201 ttaggtaagc cgcaactaaa ctaaaggctg cgaataagaa aggaagcgct aaaagagtcg
    52261 catagcctcc accaaaagca agacctgtaa ctagcatgta aaaaatagtc accagatgtc
    52321 cagcttggtc tccttgctct gtcaaggtgt ccgctgatct aaaaaggaaa aatcctcctg
    52381 ctaccaaggc taaccactca aagatggcaa agaaaaatcc gccctgactc acgtaagtca
    52441 gcaaataata aagcaaacct tgacttccat aatagtcgct gtaaatcttc cctgtctgat
    52501 gaagcgccca acctgcataa aaatcctgca cttcttgtgc actcattaag tcgagtaata
    52561 gcggtactcc tagagttatc cccgttacaa gcgtactcca tagtaaaatt ttcaccaaag
    52621 gaagacgact tgattcacga tgatgcgatt cttgttcgat ttggtattct agaggttcac
    52681 gattctcctt atgaacttct tctactctac catacacact catatcgttt ctcctgttca
    52741 atttatctgt cttagtatat caaaaagtcc taggattgtc agcttgcgat ggctgtttga
    52801 gtgatttttt gcatagtttc acaaaggttt caatttcccg aaagcggtgt aaatgtgtcc
    52861 gtttgaactc aatcatatca ttcacttctt ccttagttag catgaattcc cctcctcttt
    52921 ctttctattc gtaaaagaca agaaaaatag gtctggtttg tatcctaagt tctagatttc
    52981 ttataagata ggggcgaata gctgggcgat ttcttttaca agcttttgat accagctcgt
    53041 tttgatagaa tgaggataga cttcctgaga atctgcaaat atatttcgaa aatctcgggc
    53101 gatttccctt atagaaggag ttttatagag taagactgca ttttcataat ggtgtaccaa
    53161 gctccggtag tcgagattga ttgtccccac caccgcaaaa tcttcgtcta ccaacatctg
    53221 cttactatga ataaaacctg gactatactc ataaatccga acaccagcag aaagaaggtc
    53281 gggataagct cctctcgtga ctaactgaat gaacttctta tctggtatgt aaggggtgat
    53341 aattcgaaca tcgacccctc tcatagctgc atttttgatt gtctctgtta aatcataatc
    53401 tataatcaaa taaggtgtcg taatatagac cgattctgtt gcttgattga ttaaactctg
    53461 ataaactttt ttccctacct gcgctcgaaa aattggcttg ggtccacttc cgtatggaat
    53521 ggttaaaccg tcactcggga tagaatgatt ttctaaatga tattgatcaa aatcactaat
    53581 ttctcctcga ttaatgtacc aagtggtcaa aaataagcgt gtcagagctt ttactgctag
    53641 tccgtctaag cgaattccac tatccttcca ataaccaaat ctctcgacgt ggttaatgta
    53701 ctcatctgcc agattgaccc caccagtata ggctatctga ccatcaacaa tcaatatttt
    53761 tctatgatct ctgttattat aagccactgt caaacgagga ataactttat tgaatttatg
    53821 ggcctcgatg cccagctgac gaagtcgatg tgcataatct cctgttaaag tagccataca
    53881 gccgatatca tcatagagca tcttaacctc tacaccctga gctacctttt gctctagtat
    53941 atctagtatg cgattccaca tcaaaccttc ttctataatg taatattcca agaaaataaa
    54001 tttctcagcc tttttaagat cttctaccat ctttttccac atagcttctc cgttaggaaa
    54061 aaatgtagag gcagtttgat catagatgtc agcattggta tccatactca ataaggactt
    54121 gatgactcca taagctgcct tgtcactttc ttttaatttc tcttttagta gctggctatt
    54181 tgcttcttgg aaatgcatag agcctagctt cttcagttgt ttgatttctt ttttggacaa
    54241 tcgcctttca ccaaacatca gatagagcaa gggaccaaat actggcacaa aggctactaa
    54301 caaccaggtt accttattct caggagtcgt attacggttg actatcgaaa tgattgtgat
    54361 gatactcagt agaatgagga cagtaatcca taaaatggga gccatgcgtc ctaaataaag
    54421 aaagagacca aaaacaagac aaagttcaag tagcataatc gaaagactaa agccatactt
    54481 ggacatcaat aattgaaatt tcctatattt catatcccct ccttgatagt ttgaatgcta
    54541 aaaacaagta attgatacta gtataacgca ggtattcggt agaaggcaag tatttatgat
    54601 tattttctag gtaaaagtaa aaaggctgaa aaatccagcc ctttctactt gcatagtctt
    54661 atttagttaa aacgatttgg tccgatgggt ctctatccat atcatctacg acgatttgat
    54721 taccgtttac agtgtaaatt ttgacatcat cgccaataat catgcgttga tttgctgaat
    54781 caaatgtaac ctgtttgact ttctgatccc cgtcagattc gagctcagtc catgtaccag
    54841 tcgttccagt taccactaaa gtgatacggt ctccgtcatc ctgaccagta taagtcccat
    54901 caatatcggt cggttgagca acaggttggt tttgtactgg agccgcttga ttttggttac
    54961 ctcctgcatt tgcagtattg gtattctgtt gagcaggctg ttggacctgc tgctgagcaa
    55021 caggctgttg aacctgttga gctctttgtg aacaagctac taagagacta aaacttgcta
    55081 gagaagcaag agaaagtgtg aatcttttta atttcatgat gaatttcctt tctgctacca
    55141 atttagagaa attttctcta accagcaatt cccctagtat aacaagttca aacaaggggg
    55201 tcaatttatc tgctcacggt ccagcaggta gccccgtact tctgagataa aatagagaga
    55261 ccctgtaacg aacagcaaat cctgagcatc tgctctttct tcaaaatcgc tgataaattc
    55321 tcggtaagaa gaaactatat cgtaacctgt cacatccctt tcgtccaaag ccccctgata
    55381 gtcaaagccg gtcaccttga gttccacctg aggcaatttt tcagtcaggt aacctaacat
    55441 cccttgataa tccttccgtt tcaaggctcc aaaaaggatt tgaggtcgat agccttcctg
    55501 ctctttttct ttgataaact caaccaagcg agtcaaggca gggaggttat gagcaccatc
    55561 caagtaaatc tgtgggcgaa tacgctccaa gcgaccagcc caatgggtct gttccaaggc
    55621 ctttcttaca gcctgttcat caacagcttc ctttccttct cccataaaaa gaagaaaagt
    55681 ttgcaacgcc aaggctgcat tctcctgctg atagactcct tctaagccta tgttcagctg
    55741 tgaaagattt agtaaagagc ttgaaaaatc accattcagc attaaaaaat cttgacctgc
    55801 ctgataaagg ttaacagcta aagattcggc ttttttctga cagacaagcc tagcttctgg
    55861 aggcaatttc gcaatcactg ccttcttacc agccttgaaa ataccagctt tctgctctgc
    55921 aattgcttct agactatcac ccaaggtttc ttgatggtca agcccaatgg aggtgatgac
    55981 agcaagctct ccagttacca cattggtcgt gtcaagtaag ccaccaattc ccacttctaa
    56041 taaaaccaaa tccacctctt gctccctaaa ataaagaaaa gcaactaagg tcagcaattc
    56101 aaaaaaggac aactgggcag gagtttgcag aagcgttttc tccatctcct tgacctgatc
    56161 agtcaaacgg ataaagtctg cgtctgctat aggttgccca ttaatgcaga ttcggtcatt
    56221 gatagagacg atatgagggg aggtaaaggt cgcaactttt ttgccatgcc ccataaataa
    56281 ttccctcata aaagcaatgg tagatccttt cccattagtc cctgttacgt ggataatagg
    56341 gtaagactgc tcaggatttc ctaacaaatc caccgcttgc tgcattcggc ccaaacctga
    56401 tcgaaaattc aaaccaatcc gactatggag ccattcttct acttcaaaca tacacatctc
    56461 cttgacaaaa gtccaatcaa ttatcgcatt aaagtatggt tactaataaa aacaaggcca
    56521 ggattttcgt cccgacctct tacctggtta gctaataact agctactatg aatgtgaata
    56581 tgggctaaaa acatccacta gatgttccaa ctcttctcaa tttctaagag ttggggtaaa
    56641 aatgttcact ggacgttcca actcttcccc atttctggag ttgggctgat acagtctccc
    56701 agacttacca gctctatctt atttaaagga gctgggctaa aaacgtcccc cgaacctacc
    56761 agttctctct tatttaaagg aactgggcta aaaacatcca ctggacgttc caactcttcc
    56821 ccatttctgg gagttgggct gatacagtct ctcagactac ctgcgtttct aatttctagc
    56881 gcagggctaa aaacgtcccc cggacgtttt tactcctcca caaagctgtt gaagacttct
    56941 tcaatcatgt tccattcgtc ttctgagtct tctgggattg gttgcaattc gccttctgtt
    57001 ccatcttcat tttcaatgaa tgagtaagct tggatttcaa cttgtccgtc ttcgtcttct
    57061 tctgcgttaa ctggtactag aagaacatag tttttaccaa attcttcttt tccatcaatt
    57121 gtcaaaagga tttcaaacaa ggtttcattt ccttgctcat ctactagtgt gattagttca
    57181 cgttcttcgt ggtcgtggtt atgatcgtgt gacatagcct ctcctttata ttaaaatttt
    57241 ctatctaaat aattttgtaa aatcagctga gctgctaact tatcaatgac tttcttgcgc
    57301 ttattgcgac tgatatctgc ttgttcaatc aacatgcgct cagcagccac tgttgtcaag
    57361 cgttcatcct gatagtctac tggtaaacca aaaaactctt ctagctttgc tccgtaggct
    57421 tgactagctt ctacgcgcgg tccacttgta ttgttcatgt ttttaggcaa gcccactaca
    57481 aatcgttcca ccttgtaagt atcaaccaat tccttaacgc ggtcaaaacc aaattggcct
    57541 tgttcttcat ttatctggat gatttcaagc ccttgagctg taaaaccaag cggatcgcta
    57601 atcgccaccc ctaccgtttt tgaaccgacg tccaatccca taattctcat aggttataga
    57661 tcgactcctt gtcctttgag gtagtagcga accaattcct caacgatttc atcacgctca
    57721 tacttacgga tttgatttcg tgcattatta taacgaggaa cgtaggcagg gtctccactc
    57781 aatacgtaac ctacgatttg gttaattggg ttgtaaccct tatcgttcaa cgaagcataa
    57841 acatctgtca aagtttcgct aatttctttt ttattggaat cgtccaattt aaaacgtact
    57901 gtttcttcag taaatcccat tctaacaccc tctttcctta gaatagtacc attatagcat
    57961 aattccttac cttctacaat tcaggcagtc tatttatttg gattttctat tgttctgtcg
    58021 cgccatttgc caatctatct gaaatatatt tgcttggttc atttttcaaa agattttcca
    58081 aaccaatatt cttcagatgt tccaactggg aagccttctt gacatccaga acttgaaaat
    58141 caaaactagt cgttgtttga agttccgttg cgctcaatag ttttgtttca agtttgaaac
    58201 ctgccaattt acgagcttca atgatagact tatccttctc ctccgcttca agaagagctt
    58261 tttgagtttc ctccactcca tgttggtcaa tataagtctt caactcatca gagactggac
    58321 gtttttgttg gatagctaga ctcaaaaaag tcttgtcttt gtaagtaatt gtttgtgtct
    58381 gctggctacc atcatctgac ttgggcaaga ccaaagtctt agttacaact gtattcttct
    58441 cagcattttc aataactggc aatgccgact gaagcgtatc tttttctgtt tttgtagctg
    58501 gtccagtttc ttttttctgt ccgcaaccaa ccaggacaaa aaggaaagct agactaacaa
    58561 gaactatttt tttcatttct ttcttctttc tttttgaaat taaaatagaa taagactggg
    58621 aagtgctccc agccttgatg tttatagagc tgcacgcaaa cgtgcttctg cattttctac
    58681 attacggaca gagcgtggta ggaaggcacg aatatcgtct tccttgtagc caacttgcag
    58741 gcgtttttca tctacaagga ttgggctctt taaaattctc ggtgtttcca taatcagatt
    58801 gagaacttca ttgacactca aatcttcaat atccactcca agggctttgg catagcgatt
    58861 tttagacgaa acgatgctgg ctattccgtt atctgttttg gttagaatat ccagtaattc
    58921 ttctctcgta attccttctt taccaaggtt ttgttcttta taacttaact ggtgggcatt
    58981 gagccaggtt tttgcttttt tacaactagt acaacttgag actgtataaa ttttaatcat
    59041 gtacctaccc ctttcgctac atgttactat cagtttagtc tattatacca taaaaaacat
    59101 ccgacttgcg acctattttt aa
//
LOCUS       DAVBGY010000006        55040 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVBGY010000006 DAVBGY010000000
VERSION     DAVBGY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 55040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.006"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            97..885
                     /gene="codY"
                     /locus_tag="VQ620_000368"
     CDS             97..885
                     /gene="codY"
                     /locus_tag="VQ620_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HET2763055.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            885..1460
                     /locus_tag="VQ620_000369"
     CDS             885..1460
                     /locus_tag="VQ620_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET2763056.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDFFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            1542..1627
                     /locus_tag="VQ620_000370"
     tRNA            1542..1627
                     /locus_tag="VQ620_000370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1576..1578,aa:Leu,seq:aag)
     gene            1910..3040
                     /gene="ugpC"
                     /locus_tag="VQ620_000371"
     CDS             1910..3040
                     /gene="ugpC"
                     /locus_tag="VQ620_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET2763057.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(3187..3333)
                     /locus_tag="VQ620_000372"
     CDS             complement(3187..3333)
                     /locus_tag="VQ620_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763058.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            3541..4299
                     /locus_tag="VQ620_000373"
     CDS             3541..4299
                     /locus_tag="VQ620_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET2763059.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGLSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMKLSGMIYDEGWLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            4386..4898
                     /locus_tag="VQ620_000374"
     CDS             4386..4898
                     /locus_tag="VQ620_000374"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET2763060.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            5080..6024
                     /locus_tag="VQ620_000375"
     CDS             5080..6024
                     /locus_tag="VQ620_000375"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HET2763061.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            6033..6710
                     /locus_tag="VQ620_000376"
     CDS             6033..6710
                     /locus_tag="VQ620_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HET2763062.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            6808..7566
                     /locus_tag="VQ620_000377"
     CDS             6808..7566
                     /locus_tag="VQ620_000377"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET2763063.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            7604..9109
                     /gene="lytC"
                     /locus_tag="VQ620_000378"
     CDS             7604..9109
                     /gene="lytC"
                     /locus_tag="VQ620_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HET2763064.1"
                     /translation="MSVTFFIGEERLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAESDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTGTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPHYSGKKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            9605..10123
                     /locus_tag="VQ620_000379"
     CDS             9605..10123
                     /locus_tag="VQ620_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET2763065.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            10253..10759
                     /locus_tag="VQ620_000380"
     CDS             10253..10759
                     /locus_tag="VQ620_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET2763066.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            10759..10929
                     /locus_tag="VQ620_000381"
     CDS             10759..10929
                     /locus_tag="VQ620_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763067.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            10961..12193
                     /gene="clpX"
                     /locus_tag="VQ620_000382"
     CDS             10961..12193
                     /gene="clpX"
                     /locus_tag="VQ620_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET2763068.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            12202..12789
                     /locus_tag="VQ620_000383"
     CDS             12202..12789
                     /locus_tag="VQ620_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HET2763069.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            12800..13180
                     /locus_tag="VQ620_000384"
     CDS             12800..13180
                     /locus_tag="VQ620_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET2763070.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            13232..14122
                     /gene="rapZ"
                     /locus_tag="VQ620_000385"
     CDS             13232..14122
                     /gene="rapZ"
                     /locus_tag="VQ620_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET2763071.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            14119..15096
                     /locus_tag="VQ620_000386"
     CDS             14119..15096
                     /locus_tag="VQ620_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HET2763072.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            15093..16004
                     /gene="whiA"
                     /locus_tag="VQ620_000387"
     CDS             15093..16004
                     /gene="whiA"
                     /locus_tag="VQ620_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HET2763073.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(16038..17006)
                     /locus_tag="VQ620_000388"
     CDS             complement(16038..17006)
                     /locus_tag="VQ620_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET2763074.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWTLAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKNSTSL"
     gene            17142..17999
                     /locus_tag="VQ620_000389"
     CDS             17142..17999
                     /locus_tag="VQ620_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HET2763075.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            17986..18765
                     /locus_tag="VQ620_000390"
     CDS             17986..18765
                     /locus_tag="VQ620_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HET2763076.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            18789..20141
                     /locus_tag="VQ620_000391"
     CDS             18789..20141
                     /locus_tag="VQ620_000391"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET2763077.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAIQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            20215..20589
                     /locus_tag="VQ620_000392"
     CDS             20215..20589
                     /locus_tag="VQ620_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HET2763078.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            20591..21439
                     /locus_tag="VQ620_000393"
     CDS             20591..21439
                     /locus_tag="VQ620_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2763079.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            21716..22483
                     /locus_tag="VQ620_000394"
     CDS             21716..22483
                     /locus_tag="VQ620_000394"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET2763080.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDDMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            22495..23679
                     /locus_tag="VQ620_000395"
     CDS             22495..23679
                     /locus_tag="VQ620_000395"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET2763081.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            23676..25547
                     /locus_tag="VQ620_000396"
     CDS             23676..25547
                     /locus_tag="VQ620_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2763082.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDSKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(25690..26874)
                     /gene="mntE"
                     /locus_tag="VQ620_000397"
     CDS             complement(25690..26874)
                     /gene="mntE"
                     /locus_tag="VQ620_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HET2763083.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLATGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPISEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            27178..29874
                     /locus_tag="VQ620_000398"
     CDS             27178..29874
                     /locus_tag="VQ620_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET2763084.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLVEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVVLATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            29887..30678
                     /gene="yghU"
                     /locus_tag="VQ620_000399"
     CDS             29887..30678
                     /gene="yghU"
                     /locus_tag="VQ620_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HET2763085.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            30794..31204
                     /locus_tag="VQ620_000400"
     CDS             30794..31204
                     /locus_tag="VQ620_000400"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET2763086.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            31216..32811
                     /locus_tag="VQ620_000401"
     CDS             31216..32811
                     /locus_tag="VQ620_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763087.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
     gene            32990..33154
                     /locus_tag="VQ620_000402"
     CDS             32990..33154
                     /locus_tag="VQ620_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HET2763088.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            33233..33961
                     /gene="yaaA"
                     /locus_tag="VQ620_000403"
     CDS             33233..33961
                     /gene="yaaA"
                     /locus_tag="VQ620_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HET2763089.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDVLALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(34239..34787)
                     /locus_tag="VQ620_000404"
                     /pseudo
     CDS             complement(34239..34787)
                     /locus_tag="VQ620_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            35108..35596
                     /locus_tag="VQ620_000405"
     CDS             35108..35596
                     /locus_tag="VQ620_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HET2763090.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISGSFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQAVQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            35593..36780
                     /locus_tag="VQ620_000406"
     CDS             35593..36780
                     /locus_tag="VQ620_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2763091.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGEQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            36773..37138
                     /locus_tag="VQ620_000407"
     CDS             36773..37138
                     /locus_tag="VQ620_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2763092.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKMAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGVPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            37154..38500
                     /gene="asnS"
                     /locus_tag="VQ620_000408"
     CDS             37154..38500
                     /gene="asnS"
                     /locus_tag="VQ620_000408"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET2763093.1"
                     /translation="MMTKRVTIIDVKDYIGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            38653..38943
                     /locus_tag="VQ620_000409"
     CDS             38653..38943
                     /locus_tag="VQ620_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET2763094.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            38955..39425
                     /gene="ssbA"
                     /locus_tag="VQ620_000410"
     CDS             38955..39425
                     /gene="ssbA"
                     /locus_tag="VQ620_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HET2763095.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            39457..39696
                     /locus_tag="VQ620_000411"
     CDS             39457..39696
                     /locus_tag="VQ620_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET2763096.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            39991..41391
                     /locus_tag="VQ620_000412"
     CDS             39991..41391
                     /locus_tag="VQ620_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HET2763097.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILIGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEENGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            41393..41752
                     /locus_tag="VQ620_000413"
     CDS             41393..41752
                     /locus_tag="VQ620_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET2763098.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            41806..42555
                     /locus_tag="VQ620_000414"
     CDS             41806..42555
                     /locus_tag="VQ620_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HET2763099.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            42545..42844
                     /locus_tag="VQ620_000415"
     CDS             42545..42844
                     /locus_tag="VQ620_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET2763100.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            42915..43850
                     /locus_tag="VQ620_000416"
     CDS             42915..43850
                     /locus_tag="VQ620_000416"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HET2763101.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            43936..44448
                     /locus_tag="VQ620_000417"
     CDS             43936..44448
                     /locus_tag="VQ620_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET2763102.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            44485..45081
                     /locus_tag="VQ620_000418"
                     /pseudo
     CDS             44485..45081
                     /locus_tag="VQ620_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            45182..45376
                     /locus_tag="VQ620_000419"
     CDS             45182..45376
                     /locus_tag="VQ620_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET2763103.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVSKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(45422..46867)
                     /locus_tag="VQ620_000420"
     CDS             complement(45422..46867)
                     /locus_tag="VQ620_000420"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HET2763104.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYIAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISLDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FRFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            46973..48595
                     /locus_tag="VQ620_000421"
     CDS             46973..48595
                     /locus_tag="VQ620_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET2763105.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            48799..50757
                     /locus_tag="VQ620_000422"
     CDS             48799..50757
                     /locus_tag="VQ620_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2763106.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            50929..52610
                     /locus_tag="VQ620_000423"
                     /pseudo
     CDS             50929..52610
                     /locus_tag="VQ620_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768988.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            52768..53862
                     /locus_tag="VQ620_000424"
     CDS             52768..53862
                     /locus_tag="VQ620_000424"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET2763107.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            53870..55036
                     /locus_tag="VQ620_000425"
     CDS             53870..55036
                     /locus_tag="VQ620_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2763108.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IHQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
BASE COUNT        16260 a        10183 c        12539 g        16058 t
ORIGIN      
        1 tgtaagtcaa tgcaagtcaa taaggaattt tcagataatt attgaaaaag agacgttttt
       61 atgttataat gatagcgatt aaattcgagg tgaaaaatgg cacatttatt agaaaaaact
      121 agaaaaatta catcaatttt gaaacgctca gaggagcagt tgcaggatga gctcccttac
      181 aacgctatta cgcgtcagtt agcggatatt attcattgca atgcctgcat tatcaatagt
      241 aagggacgtc tgcttggcta ttttatgcgt tataaaacaa atacagatcg cgtagagcaa
      301 ttcttccaaa ctaagatttt cccagatgac tacgttcaag gggctaatat gatttacgaa
      361 acagaagcaa acttgcctgt tgagcatgat atgagtattt tccctgttga gagtagagat
      421 gattttccag atggcttgac gactattgca ccgattcatg tatcggggat tcgccttggt
      481 tctttgatta tttggcgtaa tgataaaaaa ttcgaagatg aggacttggt tcttgttgag
      541 attgccagta ccgttgttgg gattcagctt cttaacttcc aacgtgaaga agatgagaaa
      601 aatattcgtc gtcgtactgc tgtcaccatg gcggtcaata ccctttctta ctccgaactc
      661 cgtgctgttt cagcaatttt aggggaatta aatggaaatg aagggaagtt gactgcgtca
      721 gtgattgcag atcgtatcgg aatcactcgc tctgtgattg tcaatgctct tcgtaaactt
      781 gagtctgcgg ggattattga aagtcgctca cttggaatga agggaaccta tcttaaggtc
      841 ttgatttcag atatttttga agaagtgaag aaaagagatt actaatgaca aaggctttaa
      901 tttcgattga ttatacagaa gattttgttg ctgatagtgg gaagctgaca gcaggtgctc
      961 cagctcaggc gatttcggat gccatcagca aggtaactcg attagctttt gaacgaggag
     1021 attatatctt ctttactatt gatgctcatg aagaaaacga ttttttccat ccggaaagta
     1081 agttatttcc tcctcataat ctgattggga cgagtggacg gaatttatat ggagatttgg
     1141 ggatatttta tcaagagcat ggttcagaca gtcgtgtctt ttggatggat aaacgccatt
     1201 actcagcttt ttcagggact gacctagata ttcgtttgag agagcgtaga gtgtctaccg
     1261 ttatcttaac aggtgtcttg acggatatct gtgtcctaca tacagctata gatgcctata
     1321 atctaggata tgacatcgag attgttaaac cagctgttgc ttccatctgg cctgaaaatc
     1381 atcaatttgc cctaggtcat ttcaaaaata cactcggagc taagttagta gatgaaaatc
     1441 taaatgaact ttctgagtaa cctggttggt gaaaatgaaa aaatttgaaa aaaagtgttg
     1501 acaagcatcc caaaagttga tatactagta aagtaatcga tgcggggatg gcggaattgg
     1561 cagacgcgca ggactaagga tcctgtgacc gctttaggtc gtgagggttc aagtccctct
     1621 ctccgcatag gaaaagagtt agctaaagct agctcttttg tatttctcta aaaaattaga
     1681 atccagtcaa atgtagtgga gaatcagcga aaatatggga aaactgttcc ttgtttctgc
     1741 ttttgtcata gaagaataat aaagattatc ttcttcaaat tctccgatat tctctaaagt
     1801 tttgtgcaag ttgcacagaa cttttttatt tttttggtca tcttgccata gaaatataaa
     1861 gcgttttcat ctataatata attatcaaaa gacaaaagga gttcacctca tggtagaatt
     1921 gaatcttaaa aatatttaca aaaaatatcc aaacagcgaa cactattcag ttgaagattt
     1981 caacttgaac atcaaagata aagaatttat cgttttcgta ggaccttcag gatgtggtaa
     2041 atcaactaca ctccgtatga ttgctggtct tgaagacatt acagaaggta ctgcatctat
     2101 cgatggcgtg gttgtcaacg acgtagctcc aaaagaccgt gatatcgcca tggtattcca
     2161 aaactacgct ctttacccac acatgactgt ttatgacaac atggctttcg gtttgaaatt
     2221 gcgtaaatac agcaaagaag acattaacaa acgtgttcaa gaagcagctg aaatacttgg
     2281 attgaaagaa ttcttggaac gtaaaccagc tgacctttca ggtggtcaac gtcaacgtgt
     2341 tgccatgggg cgtgcgattg tccgtgatgc gaaagtattc ttgatggacg aacctttgtc
     2401 aaacttggat gccaaacttc gtgtatcaat gcgtgctgaa atcgctaaaa ttcaccgtcg
     2461 tatcggagct acaactatct atgtaactca cgaccaaaca gaagcgatga cacttgcaga
     2521 ccgtatcgtt attatgtcag ctactaagaa ccctgctggt acaggtacta tcggacgtgt
     2581 agaacaaatc ggtactcctc aagaagttta caaaaatcca gttaacaaat tcgttgcagg
     2641 attcatcgga agcccagcta tgaacttcat caacgtgaaa ttggttggta gcgaaattgt
     2701 ttctgacggt ttccgtttga aagtgccaga aggagcattg aaagttcttc gtgaaaaagg
     2761 ctacgaagga aaagaattga tctttggtat ccgtccagaa gacgtgaatg cagaacctgc
     2821 tttccttgaa acattcccag actgtgttgt aaaagcgact atctctgtat cagaactgct
     2881 tggttcagaa tctcaccttt actgccaagt tggtaaagac gagtttgttg caaaagttga
     2941 tgctcgtgac tacttgcaaa caggtgcaac agttgagctt ggatttgact tgaacaaagc
     3001 acacttcttc gatgtagaaa ctgaaaaaac aatctactaa aataaataaa attcaaagca
     3061 ctacaagaaa agatatctct ttatcaattg tagtggagag atatcagtta atctagggag
     3121 agaaacaaaa tgcttctctc ctttttgcta gggaagtcat attatgcatc tatattgtga
     3181 tgctctttaa tactcttcga aaatctcttc aaaccacgtc aacgtcgcct tgccgtacgt
     3241 atgattactg atttcgtcag ttttatctgc aacctcaaag atgtactttg agcagcttac
     3301 ggctagtttc ctagtttgct ctttgatttc cattgagtat tatttgtggg taccatctac
     3361 aagtgaagct atatgcgtaa actacgtgag caattgaatt cgaactagag aggtaataat
     3421 aaatttatgc tatagttatg gtgacttgta tgcttttgat tctagtttat caaataatag
     3481 attagaattg tcagataata tcattttgtg ttataatgaa gaaaaaacag aggtgttcaa
     3541 atgtcagaag caggtcataa gtttttagca aaattgggga aaaaacgctt acgtccaggt
     3601 ggaaagcgtg ctacagattg gttaattgca gaaggaggat tgtcaaaaga aaagagaata
     3661 ctagaggttg cgtgtaatag gggaactaca gcaattgagt tggcacagcg ttttggttgc
     3721 aagataactg ctgttgatat ggatgctcaa gctttagaag tggctaaaaa atctgctgga
     3781 acggcaggtg ttgctcattt aatcagtttt gaaagagcaa atgcaatgaa acttccttat
     3841 caagatgcta gttttgatat tgttataaat gaagctatgc tgactatgca agccgatcaa
     3901 gctaagaaaa aatgtgtaat ggaatatcta agggtattaa aacctggagg tcttcttttg
     3961 acacatgatg tgcttcttaa ggaagctaaa gagtctatca gacaggaatt atcacaagca
     4021 attcatgtaa atgtaggtcc tttaactcaa gatggttggg aacaggtgat gatagaatca
     4081 ggttattgtg atgtgaaagt attgactggt gaaatgacat taatgaaatt atcgggtatg
     4141 atttatgacg aaggttggct aggaactttg aaaatttgtg taaatgcttg taaaaaggag
     4201 aatagaaagc agtttttaac tatgtataaa atgtttgcta agaataaaca gaaattgggc
     4261 tttattgcga tggctagtta taaatcgtca aaacgttaga taattattga agttaacttt
     4321 tccttttttc tttcttaaaa aatatgctat aatagagagt gaaaaacttt gaaagaaaga
     4381 aaaagatgaa tttaaaagat tacattgcaa caattgaaaa ttatccaaag gaaggcatta
     4441 ccttccgtga tattagtcct ttgatggctg atggaaatgc ttatagctac gctgttcgtg
     4501 aaatcgttca gtatgctact gacaagaaag tcgacatgat cgtgggacct gaagctcgtg
     4561 gatttatcgt gggttgtcca gttgcctttg agttgggaat tggttttgcg cctgttcgta
     4621 agccaggtaa attgccacgc gaagttattt ctgctgacta tgaaaaagag tacggtgtcg
     4681 ataccttgac tatgcacgcg gatgccatta agccaggtca acgtgttctt attgtagatg
     4741 accttttggc gacaggtgga actgttaagg caactatcga gatgattgaa aaacttggtg
     4801 gtgttatggc aggttgtgcc ttccttgttg aattggatga attgaacggc cgtgaaaaaa
     4861 tcggtgacta cgactacaaa gttcttatgc attattaatg aaaacagtcc ctagggctgt
     4921 tttctctaca ctaggatata aaaatagact ataactagtt agagaaaaac tataattgaa
     4981 aactatatct tcttgcagta taataaaagg actaagtgtt tgagatttgt cttcaaacac
     5041 atgcaattat tcctgagaga gtacagttag gagagggtta tgccgattcg aattgataaa
     5101 aaattgccag ctgttgagat tttacggaca gagaatatct ttgtcatgga tgatcaacgt
     5161 gctgcccacc aagatatccg tcctttgaag attttaattt taaatctcat gccacagaaa
     5221 atggtcacag agacccagtt gttgcgtcac ttggctaata cacccctaca actggatatt
     5281 gattttctct atatggagag ccaccgttct aaaacaactc gttcagagca catggagacc
     5341 ttctataaaa cttttcctga agtcaaggat gagtattttg atgggatgat catcacgggt
     5401 gctccagttg agcatttacc atttgaggaa gtggactatt gggaggaatt taggcagatg
     5461 cttgagtggt ctaagacaca tgtctattcg acccttcata tctgttgggg ggctcaggct
     5521 gggctttatc tgcgctatgg tgtagaaaaa taccagatgg acagtaagct atcaggtatt
     5581 tatcctcagg acaccctaaa agagggtcac cttctattta gaggctttga tgatagctat
     5641 gtatcccctc attcacggca cacggagatt tctaaggaag aggtcttaaa caagaccaat
     5701 ctcgagattt tatcagaagg acctcaggtt ggggtttcta ttttggccag tcgtgattta
     5761 cgagaaattt atagttttgg tcatttggag tatgaccgtg ataccttggc aaaagagtat
     5821 tttcgagatc gtgatgcagg tttcgaccca catattccag aaaattactt taaagatgat
     5881 gatgttaatc aggtaccttg tctttgttgg tcttcatctg cagccctctt tttcagtaat
     5941 tgggtaaacc atgcggtcta tcaggagacg ccttttgatt ggagaaagat agaagatgat
     6001 gcatctgcat atgggtattt ataagaggaa ttatgacata tttagacgct tttaaatcag
     6061 gtaccttggt tttaccgagt gccctgctct tgcattttaa ggaactcttt ccttctagcg
     6121 acgattttct ggtttggcaa tttttctatt tgcaaaatac gacaggctta gaagaaatgt
     6181 cgccaagcca gattgctgaa aggattggca aggaaatttc ggatgtcaac cagtccattt
     6241 ctaatctgac ggaaagggga ctgctccagt atcgtactat cgaattaaat ggcgaaattg
     6301 aattgctctt tgatgctagt ttggccttgg aacgtttgga tgacctgttt ggagcagttc
     6361 gttcaagttc agaccagcta acacctcaaa accagctcaa ggatttggtg gaaaccttcc
     6421 agcaggagtt gggacgattg ttgacgcctt ttgagattga ggatttgacc aagacactaa
     6481 aggaagatgg aaccagtgct gacttgatta aggaggctct tcgtgaagct gttttgaatg
     6541 gaaaaccaaa ctggaagtac attcaggcga ttttgagaaa ctggcgccat gaaggaatca
     6601 agagtgtggc tcaaattgag gccaagagag cagaaagaga agcaagcaat cctcagttga
     6661 cacaggtatc tgcagatttc agaaatgcca tggatctctg gaaggattaa tccatgcaag
     6721 taggcttgaa atccgagtaa gatttgcaag ctgtgtataa ttgtgataga ataaatagaa
     6781 aataaattga aaaaagaggt atgtgaaatg tcacgtaaac catttatcgc tggtaactgg
     6841 aaaatgaaca aaaatccaga agaagctaaa gcattcgttg aagcagttgc atcaaaactt
     6901 ccttcatcag atcttgttga agcaggtatc gctgctccag ctcttgattt gacaactgtt
     6961 cttgctgttg caaaaggctc aaaccttaaa gttgctgctc aaaactgcta ctttgaaaat
     7021 gcaggtgctt tcactggtga aactagccca caagttttga aagaaatcgg tactgattac
     7081 gttgttatcg gtcactcaga acgccgtgac tacttccatg aaactgacga agatatcaac
     7141 aaaaaagcaa aagcaatctt tgcgaacggt atgcttccaa tcatctgttg tggtgaatca
     7201 cttgaaactt acgaagctgg taaagctgct gaattcgtag gtgctcaagt atctgctgca
     7261 ttggctggat tgactgctga acaagttgct gcctcagtta tcgcttatga gccaatctgg
     7321 gctatcggta ctggtaaatc agcttcacaa gacgatgcac aaaaaatgtg taaagttgtt
     7381 cgtgacgttg tagctgctga ctttggtcaa gaagtcgcag acaaagttcg tgttcaatac
     7441 ggtggttctg ttaaacctga aaatgttgct tcatacatgg cttgcccaga cgttgacggt
     7501 gcccttgtag gtggtgcgtc acttgaagct gaaagcttct tggctttgct tgactttgta
     7561 aaataatcag taagtagcaa aagctaggtg gaacagcatt cagatgtctg ttacattttt
     7621 tataggagag gaaagattga aaacaaaaat tggattagca agtatctgtt tactaggctt
     7681 ggcaactagt catgtcgctg caaatgaaac tgaagtagca aaaacttcgc aggatacaac
     7741 gacagcttca agtagttcag agcaaaatca gtcttctaat aaaacgcaaa cgagcacaga
     7801 agtacagact aatgctgctg cccactggga tggggattat tatgtaaagg atgatggctc
     7861 taaagctcaa agtgaatgga tttttgacaa ctactataag gcctggtttt atattaattc
     7921 agatggtcgt tactcgcaga atgaatggca tggaaattac tacctgaaat caggtggata
     7981 tatggcccaa aacgagtgga tctatgacag taattacaag agttggtttt atctcaagtc
     8041 agatggggct tatgctcatc aagaatggca attgattgga aataagtggt actacttcaa
     8101 gaagtggggt tacatggcta aaagccaatg gcaaggaagt tatttcttga atggtcaagg
     8161 agctatgatg caaaatgaat ggctctatga tccagcctat tctgcttatt tttatctaaa
     8221 atccgatgga acttatgcta accaagagtg gcaaaaagtg ggcggaaaat ggtactattt
     8281 caagaagtgg ggctatatgg ctcggaatga gtggcaaggc aactactatt tgactggaag
     8341 tggtgctatg gcgactgacg aagtgattat ggatggtgct cgctatatct ttgcggcctc
     8401 tggtgagctc aaagaaaaga aagatttgaa tgtcggctgg gttcacagag atggtaaacg
     8461 ctatttcttt aataatagag aagaacaagt gggaaccgaa catgctaaga aaatcattga
     8521 tattagtgag cacaatggtc gtatcaatga ttggaaaaag gttattgatg agaacgaggt
     8581 ggatggtgtc attgttcgtc taggttatag cggtaaagaa gacaaggaat tggcgcataa
     8641 cattaaggag ttaaaccgtc tgggaattcc ttatggtgtc tatctctata cctatgctga
     8701 aaatgagacc gatgctgaga gtgacgctaa acagaccatt gaacttataa agaaatacaa
     8761 tatgaacctg tcttacccta tctattatga tgttgagaat tgggaatatg taaataagag
     8821 caagagagct ccaagtgata caggcacttg ggttaaaatc atcaacaagt acatggacac
     8881 gatgaagcag gcgggttatc aaaatgtgta tgtctatagc tatcgtagtt tattacagac
     8941 gcgtttaaaa cacccagata ttttaaaaca tgtaaactgg gtagcggcct atacgaatgc
     9001 tttagaatgg gaaaaccctc attattcagg aaaaaaaggt tggcaatata cctcttctga
     9061 atacatgaaa ggaatccaag ggcgcgtaga tgtcagcgtt tggtattaag cgatgatttg
     9121 aaagagggat gtgatagtag caccctcttt ttctttgttt tatgatagtt catcctcgag
     9181 taaattcaag ttcttgctcg gaaatgaagc ttatatagta gattgaatat agacaaatac
     9241 cttgtgattg gtaaaacatt ttagaaattc atttaccttt cctaatcgac ttggtttcat
     9301 cttatttcaa tctattatag tattggggaa tttcttcaaa ccacatcagc ttggtcagtt
     9361 ctacctgcga cctcaaaact tgtgctttgg tcaagctggg tttagtttcc tagtttgctg
     9421 atggatttcc attgactata agcatccaac cctctttttg tcttctaaag aattcttaaa
     9481 ttatcagtct attgcaactt ttctcatata agttctttgt cttgctattg gttttcctta
     9541 gtagtatact aaggtagtaa tcattaagaa gtggttacaa aaaataatga atgaggtaaa
     9601 gaaaatggta gaattgaaaa aagaagcagt aaaagacgta acatcattga caaaagcagc
     9661 gccagtagca ttggcaaaaa caaaggaagt cttgaaccaa gctgttgctg atttgtatgt
     9721 agctcacgtt gctttgcacc aagtgcactg gtatatgcat ggtcgtggtt tccttgtatg
     9781 gcatccaaaa atggatgagt acatggaagc tcttgacggt caattggatg aaatcagtga
     9841 acgcttgatt acactcggtg gaagcccatt ctctacattg acagagttcc ttcaaaatag
     9901 tgaaatcgaa gaagaagctg gtgaataccg taatgttgaa gaaagcttgg aacgtgttct
     9961 tgttatctac cgttacttgt cagaactttt ccaaaaaggt ttggatgtca ctgatgaaga
    10021 aggtgacgat gtgacaaacg gtatctttgc aggcgctaaa actgaaacag ataaaacaat
    10081 ttggatgctt gcagccgaac ttggacaagc acctggtttg taagaaataa ggaaacatag
    10141 ataaatctgt aggaaatttc ttacagattt tttctatttt gtaagctatt ccaaaccagt
    10201 ctttttttga gttttttgat aaaatagtac tatcagtgaa aaggatggaa gcatgactaa
    10261 gaaaatcgta gctatttggg cccaggatga agagggtgtg attggtaagg aaaatcgtct
    10321 gccttggcat ttgccagcag aattgcagca ctttaaagaa acaactctga atcatgctat
    10381 cttgatgggg cgtgtgacct ttgatgggat ggggcgtcgc ttgcttccaa aacgggaaac
    10441 cttgattttg acgcgtaatc cggaagaaaa gatagatggg gttgctactt ttcaggacgt
    10501 ccagtctgtt cttgactggt atcaggctca agaaaagaat ctctacatta tcggtgggaa
    10561 gcaaattttt caggcttttg aaccttacct tgatgaagtg attgtcactc acattcatgc
    10621 tcgggtggaa ggagatacct atttccctga agaacttgac ttgtctcttt ttgagacagt
    10681 ttcaagcaaa ttttacgcca aagatgagaa gaatccttat gattttacca tccaataccg
    10741 caagagaaag gaagtctaat ggaacggagt ttatttggtc tattcacggc ttttctgtgt
    10801 ttcatctgct ttctggcagg agcacaagct tttcgtaaaa agcgttacgg gctttctatc
    10861 ctcctttggt taaatgcctt taccaatctg gtcaatagta ttcatgcttt ttacatgacc
    10921 ttattttaga tagaatgaca gtatagaatg gaaggaaatc atgtctacaa atagaaaaaa
    10981 tgatatgatg gtttattgct cattttgtgg caaaaaccaa gaagaagtac aaaaaataat
    11041 tgctggcaac aatgctttta tttgtaatga atgcgtggag ttagctcagg aaatcattcg
    11101 agaagaattg gttgaggaag tcttggcaga cttgtctgag gtgccaaaac caattgaact
    11161 cctccatatc ttgaaccact atgtaattgg tcaagatcgt gccaagcgtg ccttggcagt
    11221 ggcggtttat aaccactaca aacgcatcaa tttccacgat acacgcgaag agtcagaaga
    11281 tgtggatttg cagaagtcaa acattttgat gattggccca actggttcag ggaaaacttt
    11341 ccttgcccag accttggcta agagcttgaa tgtacctttt gctattgcgg atgcgacagc
    11401 tctgacggag gctggttatg tgggtgagga tgtggaaaat atactcctca aactcttgca
    11461 ggttgctgac tttaacatcg aacgtgcaga gcgtggcatt atctatgtgg atgaaattga
    11521 caagattgcc aagaagagtg agaatgtgtc tatcacacgt gatgtttctg gtgaaggggt
    11581 gcaacaagcc cttctcaaga ttattgaggg aactgttgct agcgtaccgc ctcaaggtgg
    11641 acgcaaacat ccacaacaag agatgattca agtggataca aaaaatatcc tcttcatcgt
    11701 gggtggtgct tttgatggta ttgaagaaat tgtcaaacaa cgtctgggtg aaaaagtcat
    11761 cggatttggt caaaacaata aggcgattga cgaaaacagc tcatacatgc aagaaatcat
    11821 cgctgaagac attcaaaaat ttggtattat ccctgagttg attggacgct tgcctgtttt
    11881 tgcggctctt gagcaattga ccgttgatga cttggttcgc atcttgaaag agccaagaaa
    11941 tgccttggtg aaacaatacc aaaccttgct ttcttatgat gatgttgagt tggaatttga
    12001 cgacgaagcc cttcaagaga ttgctaataa agcaatcgaa cggaagacag gggcgcgtgg
    12061 acttcgctcc atcatcgaag aaaccatgct agatgttatg tttgaggtgc cgagtcagga
    12121 aaatgtgaaa ttggttcgta tcactaaaga aactgtcgat ggaacggata aaccgatcct
    12181 agaaacagcc tagaggtgac tatggaactt aatacacaca atgctgaaat cttgctcagt
    12241 gcagctaata agtcccacta tccgcaggat gaactgccag agattgccct agcagggcgt
    12301 tcaaatgttg gtaaatccag ctttatcaac actatgttga accgtaagaa tctcgcccgt
    12361 acatcaggaa aacctggtaa aacccagctc ctgaactttt ttaacattga tgacaagatg
    12421 cgctttgtgg atgtgcctgg ttatggctat gctcgtgttt ctaaaaagga acgtgaaaag
    12481 tgggggtgca tgattgagga gtacttaacg actcgggaaa atctccgtgc ggttgtcagt
    12541 ctagttgacc ttcgtcatga cccgtcagca gatgatgtgc agatgtacga atttctcaag
    12601 tattatgaga ttccagtcat cattgtggcg accaaggcgg acaagattcc tcgtggtaaa
    12661 tggaacaagc atgaatcagc aatcaaaaag aaattaaact ttgacccaag tgacgatttc
    12721 atcctctttt catctgtcag caaggcaggg atggatgagg cttgggatgc aatcttagaa
    12781 aaattgtgag gaaaagaaaa tggcaaaaac aattcataca gataaggccc caaaggctat
    12841 cggaccctat gttcaaggaa aaatcgttgg caaccttttg tttgctagcg gtcaagttcc
    12901 cctatcccct gaaactgggg aaattgtagg agagaatatc caagaacaga cagagcaagt
    12961 cttgaaaaac atcggtgcta ttttggcaga agcaggaaca gactttgacc atgttgtcaa
    13021 aacaacttgc ttcttgagcg atatgaacga ctttgttcct tttaatgagg tttaccaaac
    13081 ggccttcaaa gaggaattcc cagctcgttc agctgtggag gtagctcgtc ttcctcgtga
    13141 tgtaaaagtc gaaattgaag tcatcgcaga gattggataa gctagttgaa gtttggtgtt
    13201 gccaaacttc ttttgatata aggagaaaaa gatgacaaag aaacaacttc acttggtgat
    13261 tgtgacaggg atgagtggcg cagggaaaac tgtagccatt cagtccttcg aggatctagg
    13321 ttatttcacc attgataata tgccgccagc tctcttgcct aagtttttgc agctggttga
    13381 aattaaggaa gataatccta agttggcttt ggtagtggat atgcgtagcc gttctttctt
    13441 ttcagagatt caagctgttt tggatgagtt ggaaaatcaa gatggtttgg atttcaaaat
    13501 cctctttttg gatgcggctg ataaggaatt ggtcgctcgt tacaaggaaa ccagacggag
    13561 tcacccacta gcagcagacg gtcgtatttt agatggaatc aagttggaac gtgaactctt
    13621 ggcacctttg aaaaatatga gccaaaatgt ggtggatacg actgaactca ctccacgtga
    13681 actgcgcaaa acccttgcag agcagttttc agaccaagaa caagcccagt ctttccgtat
    13741 cgaagtcatg tctttcggat ttaagtatgg aatcccgatt gatgcggact tggtctttga
    13801 tgtccgtttc ttgccaaatc cctattatct accagaactg agaaaccaaa cgggtgtgga
    13861 tgaacctgtt tatgattatg tcatgaacca tcctgagtca gaagactttt atcaacattt
    13921 attggccttg attgagccga ttctgccaag ttaccaaaag gaaggtaagt ccgttttgac
    13981 cattgctatg ggatgtacgg gtggacaaca ccgtagtgtg gcatttgcta aacgcttggc
    14041 gcaggactta tccaagaatt ggtctgttaa tgaagggcat cgcgacaaag accgcagaaa
    14101 ggaaacggta aaccgttcat gagaaaacca aagataacgg tgattggtgg agggactgga
    14161 attcccgtca ttctaaaaag tctgcgggaa aaagatgtgg aaatcgcagc tatcgtgacg
    14221 gtggcagatg atggtggttc ttcaggtgaa ctccgaaaaa atatgcaaca gttgacaccg
    14281 ccaggtgatc ttcgtaatgt ccttgtggcc atgtcggata tgcctaagtt ttatgagaag
    14341 gtctttcagt atcggttctc tgaggatgcc ggagcctttg ctggccatcc attgggaaat
    14401 ctcatcattg ctggcttgtc agaaatgcag ggttcaacct ataatgccat gcagttattg
    14461 agcaaatttt tccatacaac agggaaaatt tatccttcca gtgaccatcc tttgaccctt
    14521 catgcagtct ttcaggatgg gacagaagtg gctggagaga gtcatattgt agaccatcga
    14581 ggcataattg acaatgtcta tgtgaccaat gccctaaacg atgatacgcc tctggccagc
    14641 cgtcgagtag tgcagaccat ccttgaaagt gacatgattg tcctagggcc aggttccctc
    14701 tttacctcta ttttgcccaa tatcgtgatt aaggaaattg ggcgggctct tttggaaact
    14761 aaggcagaaa ttgcctatgt ctgcaatatc atgacccaac gtggggagac ggaacacttt
    14821 acagatagcg accacgtgga agtcttgcat cgtcaccttg gtcgcccttt tatcgacact
    14881 gtcttggtga atattgaaaa agtgcctcag gaatacatga attccaaccg ttttgatgaa
    14941 tacttggtgc aagtggaaca cgattttgta ggtctttgta agcaagtttc gcgcgtgatt
    15001 tcatctaact tccttcgtct ggaaaatggc ggtgccttcc acgatggaga tttgattgtg
    15061 gacgagttga tgcgcattat acaggtgaaa aaatgagttt cacagtagca gtaaaagaag
    15121 aaatcctggg ccaacaccat ctgagccggc atgaattatc tgccattatc aaaatgtctg
    15181 gtagcatcgg tctctcgact tcgggcttga ctttgtcagt tgtgacagaa aatgccaaac
    15241 tggcccgtca cctctatgag tcctttctcc atttctatga aatcaaatct gaaattcgtc
    15301 accaccaacg gagcaatctt cgcaagaatc gcgtctatac cgtttttaca gatgaaaagg
    15361 tgcaggattt gttaagtgat ttgcacttgg cagactcttt ctttggcctg gaaacaggta
    15421 ttgatgaggc gattttatcg gatgaggaag caggtcgtgc ctatctctgt ggcgctttct
    15481 tggcaaatgg gagcattcgt gaccctgagt caggcaagta ccagttggaa atcagttctg
    15541 tttatctgga ccacgcgcaa ggaattgcct cccttctcca acagttttta ctggatgcca
    15601 aggtgcttga gcgcaagaag ggggctgtga cctatctcca gcgagcagaa gacattatgg
    15661 acttcttgat agtaatcgga gccatgcagg cacgtgatga ttttgagcgg gttaagattt
    15721 tgcgagaaac ccgtaacgac ctcaatcggg ctaataatgc cgagacagct aatatcgctc
    15781 ggacagtttc tgccagcatg aagaccatta acaatatcag taaaataaaa gatatcatgg
    15841 gcttagaaaa tttgccagta gatttgcagg aagtggcgca actgaggatt cagcacccag
    15901 actactctat ccagcagtta gcagatagtc tcagcacccc tctgaccaaa agtggtgtca
    15961 atcacagact cagaaaaatc aataagatag cggatgaatt ataaaaccac gaatcctatg
    16021 tgactcgtgg ttctttttta taaactggta gagttttttg gttgtacttt ttgttcaggg
    16081 tcaatgtagt tgatagcgtt gttgacagca gttggagctt ctccgaggcc tgtcgcaatc
    16141 agatcaattt ttccgtcata gtagcagcag tcaccgatag catagatacc tgcttggctg
    16201 gattcctgtt tgctgttgac gataatcttg tgacggttga ggtcgagccc ccagttttta
    16261 aggttaccga cagaagattt gaaaccatag ttgacaaaga ggtggtctag gtcaatggtt
    16321 tcagtttcat cagatttgac ttttgtgatt tcaagtttat caagtgtttt tccatttcca
    16381 aggagttggc taggggcgaa tggtgtcttg atggttacag atgattcttg caaggcttga
    16441 acactgtgtt ccaaggcacg gaaattatct ctgcggtgaa caagggtagt tggtgcgatt
    16501 ttttcaaaag ccaaagtcca atccacagcc gagtctcccc caccaagaat cgtcactttt
    16561 ttaccagcgt attgctgaat gttagaaacg tggtagtgga tattttcata gccctcaacc
    16621 ccttcaagtt ccagcggacg tggtttgaag gcaccgccac ccatagcgat gataactgtt
    16681 ttagtcaggt gacttccttt agaagttgtg atggcaaatt cttcttcttg tttgtcaatc
    16741 tcaagaaccg tttcattgag atgaataggg gtatcaaatc catttagctg ttcaatcaag
    16801 cggttagtca actcttctcc agtcaggttt gggaagcctg gtacgtctag gatttccttt
    16861 tcagggtaga gaatagcagg ttgtccacct agctggggaa gagagtcgat gatttgaacc
    16921 ttggcttggc gtaggtgggc ataaaaggct gcaaaaagcc cgacaggacc accacccaca
    16981 atggtaatat catagagttg agacatagtt tctcctttgt tttttctagt cagtttattt
    17041 tatcatattt ttgttcaatg taaaatggac taggataggg aaaaaaatgc cagaaaatgg
    17101 tataatagga cgaaagtgtt ttgataggga ggatatcagg gatgaatttt caacaattat
    17161 ccaatttgca atattggacc agtttgtttg caagtccatg gacgatagct atcaatctga
    17221 ttgatatttt gattgttgct tatattttat accattttac aaaagctatt gcaggaacca
    17281 agattatgat tttggtacgt ggagttttgg tatttatttt agctcaaatc cttgcaaata
    17341 tgattggttt gactacgatt tcttggttaa tcaatcaaat tattacttat ggggttattg
    17401 cggcggttgt tatcttctct ccagagattc ggactggttt ggaacgtttg ggaagagcga
    17461 cagatttctt ttccaatgcc cctattagtg ctgaggaaca gatgattcgt gcctttgtta
    17521 agtctgttga atacatgagt cctcgtaaaa tcggggcctt ggttgctatt cagcgtgtac
    17581 gtaccttgca ggagtatatt tcgacaggaa ttcccttgga tgctaagatt tctgcagaac
    17641 ttctcattaa catttttatt cccaacactc ccctacatga cggtgcggtg attatcaaag
    17701 aagaacgtat cgctgtgacg tctgcctatc tgcccttgac aaaaaacaca gggatttcca
    17761 aggaatttgg gaccagacac cgggcggcta tcggtttatc agaagtctca gatgccttga
    17821 cttttgtcgt atcagaggaa acgggaggaa tttcgataac ctataatgga aggtttaagc
    17881 acaacctaac acttgatgaa tttgaaacag aattacgtga aatcttactt ccaaaagagg
    17941 aagtaggtct tagttttaaa gaacgattgc taggaggatg gaaacatgaa aaaaaatagt
    18001 ttatatatca tatcctcact cttttttgct tgtgtcttat ttgtctatgc tacggcgacg
    18061 aattttcaaa acagtaccag tgctaggcag gtaaaaacgg aaacctatac taatacagta
    18121 acaaatgtcc ctattgacat acgctataat agtgataagt attttattag cggttttgct
    18181 tcagaagtat cagtggtctt gactggtgca aatcgcctat cgctagctag tgaaatgcaa
    18241 gaaagtacac gtaaattcaa ggttactgct gacctaacag atgccggtgt tggaacaatt
    18301 gaagttcctt tgagcattga agatttaccc aatgggctga ccgctgtggc gactccgcaa
    18361 aaaattacag tcaagattgg taagaaggct cagaaggata aggtaaagat tgtaccagag
    18421 attgacccta gtcaaattga tagtcgggta caaattgaaa atgtcatggt gtcagataaa
    18481 gaagtgtcga ttacgagtga ccaagagaca ttggatagaa ttgataagat tatcgctgtc
    18541 ttgccaacta gcgaacgcat aacaggtaat tacagtggtt cagtaccttt gcaggcaatc
    18601 gaccgcaatg gtgttgtctt accggcagtt atcactccgt ttgatacaat aatgaaggtg
    18661 actacaaaac cagtagcacc aagttcaagc acatcaaatt caagtacaag cagttcatcg
    18721 gagacatctt cgtcaacgaa agcaactagt tcaaaaacga attaaaaata gaaaaggatt
    18781 ttataacaat gggtaaatat tttgggactg atggagtccg tggagaagct aacctagaac
    18841 taacaccaga attagccttt aaactaggac gttttggagg ctatgttctt agtcaacatg
    18901 aaacggaagc gccgaaagtc tttgtaggac gtgacacacg tatttcaggg gaaatgctag
    18961 aatcggcctt ggtggcaggt ctcctttcag tagggattca cgtatacaaa cttggtgtcc
    19021 ttgcaacacc agcagtagct tacttggttg aaactgaagg agcaagtgct ggtgtcatga
    19081 tttctgctag ccacaatcca gctcttgata acggaatcaa gttctttggc ggtgatggct
    19141 tcaaactaga tgatgaaaaa gaagcagaaa ttgaagcctt gctagatgct gaggaagaca
    19201 ctcttcctcg tccaagtgca gaaggcttag gaattttggt agattatcca gaaggcttgc
    19261 gtaagtatga aggatacctt gtttcaactg gaactcctct tgatggaatg aaggttgcct
    19321 tggatacagc taatggagca gcttctacca gtgcccgtca aatctttgca gaccttggtg
    19381 cccaattgac ggttatcggg gaaacaccag acggtcttaa catcaacctt aatgttggtt
    19441 caacacatcc agaagccctt caagaagtgg tcaaagaaag tgggtcagct attggtttgg
    19501 cctttgatgg agacagtgac cgcttgattg ctgttgatga gaatggtgac atcgtcgatg
    19561 gtgacaagat tatgtacatc atcggaaaat acctttctga aaaaggacaa ttggctcaaa
    19621 atacaattgt gacaactgtt atgtctaacc ttggtttcca caaggccttg aatcgcgaag
    19681 gtattaacaa ggcagttact gcagttggtg accgctacgt tgttgaagaa atgagaaaat
    19741 caggttacaa ccttggtggt gaacagtctg gtcacgttat cttgatggat tacaatacca
    19801 caggtgatgg tcaattatca gcaattcaat tgactaaaat catgaaggaa actggtaaga
    19861 gcttatcaga gttggcggca gaagtaacga tttatccaca aaaattagtt aatatccgag
    19921 tggaaaacgt catgaaggaa aaggccatgg aagtgccagc tatcaaggcc atcatcgaga
    19981 agatggaaga agaaatggcg gggaacggcc gtatccttgt tcgtccaagt ggaacagagc
    20041 ccctcttgcg tgttatggca gaagcgccta caacagaaga agtggactac tatgttgata
    20101 ctatcacaga tgtagttcgt gctgaaattg ggattgacta aatcctagaa aactagatga
    20161 aaataaaaaa ttatggtaca atagcttata atattgtagc cgatggagaa agacatgaat
    20221 cttaaacgtg aacaagaatt tgttagtcag tatcattttg atgcacgtaa ttttgaatgg
    20281 gaaaatgaaa atggagctcc tgaaaccaag gtggatgtga atttccaatt gctccaacat
    20341 gaccaagaaa atcaagtgac ttccttgatt gtcatcttga gctttatgat tgtatttgat
    20401 aagtttgtca ttagtggaac aatctcacaa gtaaaccata tcgatggccg tattgtcaat
    20461 gaaccaagtg aattgaacca agaagaggtg gaaaccttgg ctcgtccatg tttgaacatg
    20521 ctcaatcgtt tgacttacga agtgactgaa attgcattag acttaccagg aattaatttg
    20581 gagttctaat atgaaattag ctgttttcac agattcctct gcttatctca gtgaagagac
    20641 cttgcaaaga gaagacttgt ttgtcttgga tattcctgtc aatattgatg gtgaggaata
    20701 tgtcgaaggc atcaatctgt ctgctgagga attttaccaa aaaatggctc aggcttctga
    20761 attgcctaag accagtcaac caagtattgc caagttagat gagatcttaa cttcgctcaa
    20821 agaacaaggc tatacacatg ccttggggct tttcctatct tctggaattt caggttttta
    20881 ccaaaatatc cagtatatgg tcgatgacta tgagggctta accattgctt tcccagacac
    20941 tttgattaca agtgctcccc taggcatcat ggttgaaagc gtctttaatt ggcgtgatca
    21001 gggcgatgat tttgccagca ttcaggataa gctagccatt caaatcagcc gtacgtcagc
    21061 ctttatcatg gtagatgact tggatcattt ggtaaaaggt ggacgccttt caaatggggc
    21121 tgccattttg ggcaatttgc ttagcattaa gccaatcctt tattttaacg atcaaggtgt
    21181 gattgaagtt tacgaaaaag ttcgtactga aaagaaggcc accaagcgct taattgaaat
    21241 tatcaaggaa acaacggctt caggtcaata ccgggtcatt gtcattcatg ggaatgctcc
    21301 tgaaaaggct gaagaattgc gtcagcactt gcttgatttt ggcttgggtt cggatgtttc
    21361 acttgctaca tttggtagtg tcattggaac gcacctagga gcaggaagta ttgctctggg
    21421 ttatattcca gtgatttagt tggcgcttct aggctagtta agatgccttg tatcttagaa
    21481 attgaacacg gactacctgc ctcttgaaaa aagatgcctg tcttgccttt cgtggaaagt
    21541 cagtgccatt ccctattttt cagaggcagc taaggtcctt tgtatcttga taattgaaca
    21601 cgcctggaac cctgtgtgaa aaagatagtt cttccaagaa gtagacactt cttgattgga
    21661 attcctattt tcactttgtg ttcttacgga ctttgtatct tagacaggag tagagatgag
    21721 tattcgagta attattgccg gttttaaggg aaagatgggc caggctgctt gtcagatggt
    21781 attgactgat ccagacttgg acttggtggc agttttggat ccttttgagt ctgagtcaga
    21841 atggcagggt attcctgttt tcaaggataa ggctgattta gctggttttg aagcggatgt
    21901 ctgggtagat tttactactc cagctgttgc ctacgaaaat acacgttttg ctcttgaaaa
    21961 tggctttgct ccagtagttg gaacgactgg tttcacgagt gaagaaattg cagagctaaa
    22021 agaattttct cgtgcccaag acttgggtgg cctgattgcc cctaactttg ccttgggtgc
    22081 tgtcttactc atgcaatttg cgacgcaggc tgccaaatat ttcccaaatg tggagattat
    22141 tgagctccat catgacaaga aaaaggatgc tccgagtgga acagccatta aaacagctga
    22201 gttgatggca gaggttcgag agtccattca gcaaggcgca gcagatgagg aagagctgat
    22261 tgctggtgct cgtggtgctg actttgatga tatgcgcatc cactcagttc gtttgccagg
    22321 cttggtagct catcaggaag tcatctttgg caatcaggga gaagggttga ccctccgtca
    22381 tgactcctat gatcgcatct ccttcatgac aggagtcaat ttgggaatta aagaagttgt
    22441 caagcgtcat gagcttgtct atggattaga acacttatta tgagattaac gcaaatgcct
    22501 tctgaatttc agaaggcttt accagtatta gaaaaaatta aagaagcagg ctttgaggct
    22561 tattttgttg ggggctctgt tcgagatgcc cttctccata gccctatcca cgatgtggat
    22621 attgcgacgt cttcttatcc agaagagacc aagcagattt ttccgcgaac agccgatatc
    22681 ggaatcgagc atggaaccgt cttggtctta gatggggatg aggagtatga ggtaacaacc
    22741 tttcggacag aggatgtcta tgtggactat cgcagaccca gtgcggtttc ctttgtacga
    22801 tcgctagaag aagacctcaa acgccgtgat ttcacagtca acgcctttgc cttggatgag
    22861 acaggagaaa tcgttgactt gttccatggt ttagaagatt tggaaaagca agtcttgcga
    22921 gcagttggag tggctagtga gcgtttcaac gaagatgctt tacggattat gcgtggtttc
    22981 cgttttcagg ctagtcttgg ttttgcactt gagccagaaa catttaaagc tatgaagacc
    23041 ttgacgccgc ttttggagaa aatttctgta gagcgtacct tcgtcgagtt tgataaactc
    23101 ttgctggctc cattttggag aaggggcttg gcttccatga ttgagagtca agcttatgac
    23161 tatctccctg atatggcatc tagccaggac aagctcaaca gactgtttga tttggagact
    23221 gattttactt ttgaatcctc tgaacaagcc tgggcggctt tactatgggc tttggagatt
    23281 gaaaatgcgc agtcattttt gaaatcttgg aagacctcac gccagtttgc caagcaagtt
    23341 caggatttgc tgattatttt ggctctgcgt gaaaatggag aattgagtaa gcgagattgt
    23401 tatcgctttg acatagattt gcttttacag gctgaaaatc ttcgtcaagc tcaaggaaaa
    23461 gaagtcaacc cacaagccat cacagaaaaa taccaaagct tgactatcca tgacaagaaa
    23521 gagattcaga ttaatggcga tattttgatc aaggaatatg gctatcagcc aggcccagac
    23581 ttgggagaga ttttaacaga gattgagttt gccattgtcg atggagaatt ggagaataat
    23641 cgtgaagcca tccatgctta cctgagggag aaaaaatgag tgattttatc gttgaaaaac
    23701 taagtaaatc cgttggtgac aagaccgttt ttagggatat ttcctttatt atccatgact
    23761 tagacagaat tggtttaatc ggtgtcaatg ggactggcaa gaccaccctt ttggacgtcc
    23821 tttctggtgt ttctggattt gatggggatg tcagtccttt ttcagctaaa aatgattacc
    23881 agattggtta cttgactcag gatcctgatt ttgatgatag aaagacagtt ttggatacgg
    23941 ttctatctag tgaactcaag gaaatccagc tcattcgtga gtatgaattg attatgctcg
    24001 actatagtga ggacaagcag gcgcgtttgg aacgtgtcat ggcagagatg gactctctcc
    24061 aagcttggga aatcgaaagt caggtcaaga ccgttcttag caaattgggc attcaagact
    24121 tatctactcc tgttggggaa ttgtcaggtg gtctgagaag acgggtacag ttggcacaag
    24181 tcttacttgg caaccacgac ctcttgcttt tggatgagcc gaccaaccat ctggatattg
    24241 cgattattga gtggctgacc ctctttttga aaaattctaa gaagaccgtc ctttttatca
    24301 ctcacgatcg ttatttctta gacgctttgt caacacggat tttcgagttg gatcgtgcag
    24361 gcttgaccga gtaccagggc aattaccagg actatgttcg cctaaaggcg gaacaggatg
    24421 agcgcgacgc ggctcttctt cacaaaaaag aacaactcta caaacaagaa ctggcctgga
    24481 tgcgcagaca accgcaggcg cgtgcgacca agcagcaggc ccgtatcaat cgattccatg
    24541 atctgaaaaa ggaagtttca ggcagtagtg ctgagacaga cttgaccatg aactttgaaa
    24601 ccagtcggat tgggaagaaa gtcatcgagt ttcaggctgt ttcctttgcc tatgaaaaca
    24661 agcctatttt gcaagatttt aatctcttgg tgcaggccaa agaccgtatc ggaatcgttg
    24721 gggacaatgg tgttggaaaa tcaaccctgc tcaacttgat tgcaggaagt cttgagccaa
    24781 ctaaaggaca agttgtgatt ggggaaactg ttcgcatcgc ctatttctct cagcaaattg
    24841 agggtttgga tgaaagcaag agagttatca attacctgca ggaagtggca gaagaggtca
    24901 agaccagtgg cggttctacg acttcgattg cagagttgct ggagcagttc cttttcccac
    24961 gttcgacgca tggaaccttg attgagaaat tgtcaggggg tgagaaaaaa cgtctttatc
    25021 tcctcaaact gcttttggaa aaaccaaatg ttcttctttt agacgagcca accaatgacc
    25081 tagatattgc aactttgaca gtcttagaga atttcttgca aggttttgca ggtcccgttt
    25141 taacagtcag tcacgaccgc tatttcttgg ataaggtagc gaccaagatt ctcgcttttg
    25201 aggatagcaa gattcgtcct ttctttggtc attacaccga ctatcttgat gaaaaagctt
    25261 ttgaaacaga tatggccaat caagtgcaaa aggtcgaaaa ggaaaaagtg gtcaaggttc
    25321 gagaagacaa gaaacgtatg acctaccaag aaaagcagga gtgggcaagt attgaaggtg
    25381 atattgaaac cttggaaaaa cgtatcgctg ctattgaaga ggaaatgcag gctaacggct
    25441 ctgactttgg taagctggct actctccaaa aagaattgga tgagaaaaat gaagcactcc
    25501 ttgaaaaata cgaacgctat gagtatctca gtgaatttga tagttaagac tattcgaaaa
    25561 tcaaagtgca aactatgtcg gcttcacatt gccgactcaa gtacagcctg tgaccggctt
    25621 cctaatttgc actttgattt ttattgagta taaaagaata gaaaaatccc gtctcatgaa
    25681 aggatttctc tattcttttt tggtttcttg atgaaagata ttttgccagg tttcgtggcg
    25741 acgccagata ctggtatgga tgataccatc taactcatag cagatgagtt tggttttttg
    25801 actgatggaa gtaatttgaa tgtccttgat agcagaattt aactcttttt tggtcttata
    25861 agcctcttta tccatctgct ctccatcttg gcgaatatag acaaaatcat cagtcaagag
    25921 ttcttctagt tggtttcctt ggtcaatcaa ttgttcacgc ataagcaatt ttttatagac
    25981 attgtctaaa atttcatcct cagagatagg tgctggttcg atatggacat cggtatcaaa
    26041 gacgccaaaa cgctcctcca gcatagactc gacctgatcc gcgatttcat ggctttcaaa
    26101 aacagacaag tcaggattca tctctagtgt aatatccagg tagatgttgc taccgtaggt
    26161 gcgacctctt tgcgatttga ccttgctgat tttgggaatt tccatgatag ccttttggta
    26221 gtcctcgagc aggcggtcgt caaagccatc tgaaagacta aaggaagact cgatgaagat
    26281 atcataggca gtcttcaaga taaagaaagt gatgatgata gcaaccagtt tatccacaat
    26341 cggataattg aaactactag ctaggatggc aatggcggtt ccaagtgagg taacagcgtc
    26401 agaaagattg tccttagcag ctgctttcag cgccttggag ttggatttct tactgaggcg
    26461 agtattgtag agatagacca caaacataat cgctgcagaa atgattccta gagttgcacc
    26521 aagaggatca atgaccgttt cttcccgact gagaatcttt tgaatggtat ctcttagaac
    26581 atcgaaaccg acatagaaca tgatgataga agtgatcaag cttgccaaat cttcaatctt
    26641 ccaatgacca aaacggtggt cacggtctgc aggctggcgc gccatccgaa tcccgattaa
    26701 gagggccaca tttccaatga tgtccgatac gttattaaaa ccatcggcca ccaaactgga
    26761 tgaatgaagg agatgaccag ttgctaattt ggctgcagac aagatcaaat aggtcgaaat
    26821 actgataatg gctccacgct cagctaactt gagatttgag atagattgtt tcattcaact
    26881 tcctttctag tcacatagca ttctaccatt atactggttt tcaaggaaaa attcaaataa
    26941 ggcagttttt actctttgaa aactccccaa aatttggtat aatagtacta ctcaagggag
    27001 tagctggcag aaacctgtga tagtgtcgtc attccgaatt ttatgctgaa aagtatgctt
    27061 tccggcccta tcttaaacag cgagacttgt tatgattaac aggtctcttt ttgtttgtcg
    27121 tgaaaagata tgacagaaag agagtctgtt ttgcacacaa tgtcaaggag gagacacatg
    27181 tcaaaagaac aaaaacgcca agcgttttat actcagagcc ctgaagaggt cttgcaggct
    27241 gtggatgcga ccgagcaagg tttgtcatca agtgaggcgg aaaagcgcct tgtcgaattt
    27301 gggcacaatg aactcgaaga aggcgagaaa cgatcaatct tggtcaaatt catcgagcaa
    27361 tttaaggatt tgatgattat catcctagtt gcggcagcaa tcttgtcagt cgtgacttct
    27421 ggtggggaag atatcgcaga tgctattatc atcctagctg tggttatcat caacgctgcc
    27481 tttggtgttt accaagaagg aaaagctgaa gaagctattg aagccctcaa atccatgtct
    27541 agtccagttg cccgcgttct tcgtgatgga cacatggcag agattgactc taaagaattg
    27601 gtaccaggcg atatcgttgc ccttgaagca ggtgacgtgg taccagcgga cctacgtttg
    27661 atagaagcca actctcttaa aattgaagaa gcagccttga caggtgaatc tgtaccagtc
    27721 gaaaaagact tgtcagtcgt gcttgcgaca gatgctggta ttggtgaccg tgtcaacatg
    27781 gccttccaaa actcaaacgt aacctatggt cgtgggatgg gtgttgttgt caatacaggt
    27841 atgtacactg aagttggtca tattgctggt atgcttcaag atgcggatga gactgataca
    27901 ccactcaaac aaaacttgaa caacctttct aaggtcttga cctatgctat cttggtcatt
    27961 gcccttgtta cttttgtagt gggtgtcttc attcaaggga aaaatccact tggtgagttg
    28021 ttgacttctg ttgcccttgc cgttgcagcc attccagaag gacttcctgc tatcgttacc
    28081 atcgttcttt ctcttggtac tcaagttttg gccaaacgac attccatcgt tcgtaagttg
    28141 ccagcagttg aaacacttgg ttcaactgaa atcatcgctt ctgataagac tggtacgctg
    28201 actatgaaca agatgacagt cgaaaaagtc ttctacgatg cggttctaca tgactcagct
    28261 gatgatattg aactaggtct tgaaatgcca ctacttcgtt cagttgtctt ggccaatgat
    28321 acgaaaatcg atgtggaagg taacttgatt ggtgacccaa ccgaaacagc ctttatccaa
    28381 tatgccttgg acaagggcta tgatgtcaaa ggtttcttag agaaatatcc tcgtgtagct
    28441 gaattgccat ttgactctga ccgtaagctc atgtcaacag ttcacccatt gccagatggt
    28501 cgtttccttg tagcagtcaa gggtgcgcca gaccaactct taaaacgttg tcttcttcgt
    28561 gataaggctg gggatattgc tccgattgat gagaaggtta caaatctcat tcatacaaac
    28621 aattctgaaa tggctcatca agccttgcgt gtccttgcag gtgcttataa gattatcgat
    28681 agtattccag aaaatctcac ttctgaagag cttgaaaatg atttaatttt tactggtttg
    28741 attgggatga ttgaccctga acgtcctgaa gccgctgagg ctgttcgtgt ggctaaggaa
    28801 gcgggaatcc gtccaattat gattacaggt gaccaccaag acacagcgga agccattgcc
    28861 aaacgtttgg gaatcattga cgcaaacgat acagaaggtc acgttttaac tggtgctgaa
    28921 ctcaatgaac tgtcagatga agaatttgaa aaagttgttg gtcaatactc tgtttatgcc
    28981 cgtgtgtctc cagaacacaa ggttcgtatc gtcaaggctt ggcaaaaaca aggtaaagtc
    29041 gttgccatga caggtgacgg tgtcaatgac gcgccagctc tgaaaacagc cgatatcggt
    29101 atcggtatgg gaatcactgg tacagaagtt tctaaggggg cttctgacat gattcttgca
    29161 gatgataact ttgcgactat tatcgtcgca gtggaagaag gacgtaaggt cttctcaaac
    29221 attcaaaaga ctattcagta cctactttct gctaatactg ctgaagtatt aaccatcttc
    29281 ctatcaacct tgtttggttg ggatgtcttg cagccggttc atcttttgtg gatcaacttg
    29341 gtaacggata ccttcccagc tatcgctctt ggtgttgaac ctgcggaacc tggtgtcatg
    29401 aatcataaac cacgtggacg caaggcaagc ttcttctcag gtggtgtttt gagttctatc
    29461 atttatcaag gtgtactcca agcagctctt gttatgagtg tttatggcct tgcgattgct
    29521 tacccagttc atgtgggtga caatcatgct attcatgcag atgccctaac gatggccttt
    29581 gcaacccttg gtttgattca gctcttccat gcctacaatg tcaagtctgt ctaccaatcc
    29641 atcttgacag ttggcccatt caagtctaag acctttaact ggtccatctt ggtatccttt
    29701 atccttctca tggcaacaat cgttgtagaa ccgcttgaag gaatcttcca cgtaaccaaa
    29761 ctagacttgt cacaatgggg aattgttatg gctggaagct tctcaatgat tatcatcgtc
    29821 gaaatcgtta agtttattca acgcaaactc ggttttgaca agaatgcgat ttaagggagg
    29881 tttcggatgt cagcatatca attaccgacc gtatggcagg atgaagctag taatcaagga
    29941 gcttttacgg ggctaaacag accaacagca ggtgcccgtt tcgaacaaaa cttgccaaaa
    30001 ggagaacaag cttttcagct ttattcactg ggaacaccaa atggtgtgaa ggttactatc
    30061 ttattggaag aattactaga agctggtttt aaggaagcgg cttacgactt gtataagatt
    30121 gctatcatgg atggggatca attcggctca gactttgtga agctcaatcc aaattccaag
    30181 attccagcct tattggacca gtcaggtact gaaaacgtaa gagtctttga gtctgctcat
    30241 attcttcttt accttgctga gaaatttgga gcctttttac caagtaatcc tgtggaaaag
    30301 gtagaagttt tgaattggct attctggcaa gcaggtgcag caccttttct aggtggggga
    30361 tttggacatt tcttcaatta tgctcctgaa aaattggaat atcctattaa ccgttttacg
    30421 atggaagtga aacgccagtt ggatttattg gataaggaat tggctcagaa accttatatt
    30481 gcaggcaatg actatacgat tgcagatatt gctatctggt cttggtatgg acagttagtt
    30541 caaggaaatc tttaccaagg ttctgcaaaa ttcttggatg cctcaagtta tcaaaatcta
    30601 gtaaaatggg cagaaaaaat tgccaatcgt ccagctgtta agcgtggctt ggaagtaact
    30661 tatacagaaa ttaaatagca aaaaagtcat cttcggatgg ctttttgcta cagttgaggg
    30721 aaagggtttg cagttatcgg cttttgtgct ataattgcta taatatagta aaaagcaaga
    30781 ggagtgtgag gaagtggaaa agaaaattgt gaaggatatc ttatttttat ctcaagtgtc
    30841 tcagccggca agtcaggagg acctttatct tgccagagat ttgcaggata cactcttagc
    30901 aaatcgtgat acctgtgttg gtctagctgc caatatgatt ggggtgcaga agcgcgtgat
    30961 tatctttaat cttggcttgg ttcccgtggt catgtttaac ccagtgcttc tgtcctttga
    31021 aggatcctat gaagcagaag aaggctgttt gtccttggta ggtgtgagat caactaagcg
    31081 ttatgaaacc ataaggcttg cctatcgtga cagcaagtgg caggaacaga ccattacctt
    31141 gacaggcttc ccagctcaga tttgccagca tgagctggat cacttggaag gacgaatcat
    31201 ttaggaggaa agcaaatgaa acgaatagtc tttgaactta tttttatcgc aacgacctgg
    31261 tatatctttt taccgcccct taacctgacc agctgggaat ttctcttctt cctctgtggg
    31321 catttgttag ttgtggcaat attatttggc tttggcaagg ggataaacct tgtcaaaacg
    31381 gttcatgtgc gccacggtaa ggcggaagct gccttaaatc ttgagggttt caaaatcaat
    31441 cggttaggga aaattctgtt agcttcgatt ggaggaattc ttctcttggc agctttggtt
    31501 tccttggtaa cttccagcat gtttcaggct aaaaattatg ccaatgtagt cacggttacg
    31561 gaaaaagact ttactgaatt tcctaagagt gacaccagta aggttcctat cctagataga
    31621 agtactgctg aaaaaattgg agaccgctac ttgggttccc taaccgataa ggtgtcgcaa
    31681 tacgtagcgg cagataccta tacccaattg acaattgatg ggaaacctta tcgggtcaca
    31741 ccactagaat atgcagaccc tatcaaatgg tttaacaatc aagccaaggg aatcggtgag
    31801 tatatcaagg tggacatggt aactggaaat gcggatttgg tggacttgaa gacaccaatc
    31861 aagtattcag actcggagta ttttaaccgt gatgtcaaac gtcacctgcg cttgaagtac
    31921 ccgaccaaaa tctttaaaac tccatctttt gaggtggacg atgagggcaa tcctttctat
    31981 gtagcaacgg tttaccaaaa gcaatttgga cttgctgttc ctcgtcctgc ttcagtcatt
    32041 atcttggatg ctacaaatgg agaaaccaag gaatacagct tatcagatgt tccagaatgg
    32101 gtggacagga tctatccagc agaggaaacc attgagcaaa tcaactacaa tggcaagtac
    32161 aaggacggtt tcttgaatgc catgatttcc aagaaaaacg tgacccagac taccaatggc
    32221 tataattact tgtctatcgg taatgacatc tatctctaca caggtgtgac gtcggctaat
    32281 gcggatgaga gtaatcttgg tttcatcctt gaaaatatgc gaacaggaga aattactaag
    32341 tatagcttgg cttctgcgac agaagaatca gcccgtgaat cagcagaagg tgctgttcag
    32401 gagaaatcct acaaagcaac cttcccaatc ctcatcaacc tcaatgacaa gcctctctac
    32461 atcatgggct taaaggacaa tgctggcttg gtcaaagagt acgccctggt agacgcagtc
    32521 gagtaccaaa atgttatcgt tgctactaca gtggaagaga tgctcagcaa gtatgccaat
    32581 aaaaacgacc ttgaaattga caatgcaacg acagaaagca tcaagggagt agtagcagac
    32641 ctcaaatcag ctgttatcaa gggagacact gtctacttct ttaaagttga tggcaagata
    32701 tacaaggtaa aggcttcagt atccgatgac cttccttacc ttgaaaatgg taaaaccttc
    32761 gaaggtcaag taggaaaaga caactatctc aagaccttta aagtacaata atactcttcg
    32821 aaaatctctt caaactacgt cagctctatc tgcaacctca aaacagtgtt ttgagcagcc
    32881 tgcggctagt ttcctagttt gctctttgat tttcattgag tataaaaaag gtttatttaa
    32941 gaaatatatg ttataataag gtaaattaag cctaatggag gacaaagaaa tgggttttta
    33001 tttgatggtt gcttcaatgc tactcggact gctcgctttg aagataggtt tttctcagtt
    33061 caaggagaaa aaagataaat tcttatctat cttaacaagt ttagctggca cagcccttgt
    33121 tctcgtggct gtctggttag gatggcccaa ataaatagaa aaacctcggt caaaccgagg
    33181 tttttcaaat gaatttcttg gttgtggcta aaaaatatgc tacactatca atatgaaaat
    33241 tttaatccca acagcaaaag aaatgaacac agacttgcca agtatcgagg caattccttt
    33301 aaaaccagaa agtcagaccg tgctggatgt cttggctctc tattctgcca gtcaattgga
    33361 gagtttctac aaggtatcag ctgagaaagc ggcggaagaa tttcaaaata tccaagcttt
    33421 gaaaaggcaa actgctcaac actatccagc cttgaaactt tttgatgggc ttatgtaccg
    33481 caacattaag agagataagc tgaccgaggc ggaacaagat tatcttgaaa atcatgtctt
    33541 cattacctcg gctttgtacg gtgttgttcc agccttgtca cccatggctc ctcaccgttt
    33601 ggattttttg atgaaattaa aagtcgctgg taagactttg aagagccatt ggaaggcagt
    33661 ctatgatgaa gctctgaaga aggaagaagt gattttctct ctcttgtcat cagagtttga
    33721 gactgtattt tctaaggaaa tcagagcaaa gatggtgacc ttcaaattca tggaggatag
    33781 aggcggtcag ctgaagattc actcaactat ctccaagaaa gcgcgcgggg cctttctaac
    33841 agctttaata gaaaatcaag tacaaactgt gggggaagca cgtcgcttga actttgctgg
    33901 atttgtttac cgagaagatt tgtcacaacc acagggattg gtttttgtaa aggaagtata
    33961 gaaatcagat attttgtatt tggcaatatg tatcatcagc tgttttagtt tataaatgag
    34021 aaaaaaagcg aacaagctag tcttctgagt gtcagaaaag tagtcttgtt cgcttttatt
    34081 atgttatact cttcgaaaat ctcttcaagc cacgtcagcg tcgccttacc gtatatatgt
    34141 cactgacttc gtcagttcta tctacaacct caaagcagtg ctttgagcag cttgcggcta
    34201 gcttcctagt ttgctttttg atttttattg agtgttagtt acttgatagc ttcgaccaag
    34261 tcttgagctt gtttttcaag tgagtttagg actgtttctt caagaaccaa ttttccgtct
    34321 gcccaggcag agtcattaac acgtgcagca gtgaaatcac caacgccttg tgtacggata
    34381 aatggcaaga ggtctttgta gatagcgaaa agttgatcgt gccctgcatt ggctacagat
    34441 gatactgtga caaacttgtc ttgaagggca gaaacgccac gtgtatcaga caagtcaagg
    34501 gcacgagata gccagtcaag caagtttttc actgtaccag ggatagagaa gttgtagact
    34561 ggagagaaaa tccagatagc atccgcaacg agaactgctt cacgagcagc agctacagct
    34621 ggatgtgttg gaacttccaa atcttggcta aagagaggaa gggctgaata atcaaggtag
    34681 ctaacttccg ctttaccagc aagtgctttc tcagcttaga gcgccatttg gtggttgaaa
    34741 gaaccttgac gtagtgatcc gacgataaat aatacttttt tagacatgat gtgtctcctt
    34801 tttcttaaaa tttcgagaat tctctcgttg ttataaatta tattacaaca aaacaaaaga
    34861 acttgcaaga aatttgctca gagtttattt gaaaattgta aaatttcgga aactctagtc
    34921 atttgtgact gaaaaagtaa gaactgtttc actttcaaaa tcggttttgt ttgaagggag
    34981 aaatagaatt ggttgctcaa agcacagctt tgaggttgca gataaagctg acgtggtttg
    35041 aagagatttt cgaagagtat tcttatattc acttgtaata aagcttaata gatggtaaaa
    35101 tagacgagtg aaaaaaagac aaaacaaagc aaaaaataat ctactgtggc agtatggtct
    35161 agggatgacg attttgtttg tggttatcag tggttccttt ctgtatatgg tttctcttag
    35221 catgaaaccc tatcaaacag ctaaaagtga aggagaaaaa ttagctcagc agtatgcagg
    35281 attagagcag gctgttcagg ttgatttata caatggcttg gaatcttatt acagcgttct
    35341 tggtcgtaat aaacagcaag aagcgcttgc tgtcctgatt ggtaaagatg accataagat
    35401 ttacgtttat cagctaaatc agggtgtttc acaagaaaaa gcagaaacgg tttctaagga
    35461 aaagggagct ggcgagattg acaagataac ctttggccgt tatcaagata agccaatctg
    35521 ggaagttaag tcaggatctg atttttatct agtagatttt gaaacaggag cattggtcaa
    35581 caaggagggc ctatgaaatt atccaaccgt gttttagaaa tggaagaaag tgtgactttg
    35641 gctgctggag cccgtgccaa agcactgaag gctgagggta gagatatttt gtctctaacc
    35701 ttgggtgagc cagatttcac tactcccaaa aatatccaag atgccgccat tgcatcgatt
    35761 cgagatgggc gtgcttcttt ttatacagta acctcaggtc tgccagagct taaggcggcg
    35821 gtcaatagct actttgagcg cttttacggc tattctgtag cgtcaaatca agtgacagtc
    35881 gctgcgggag ccaaatattc tctctatacc ttctttatgg ctgtggtcaa tccaggtgat
    35941 gaagtcatca tcccaacccc atactgggtt agctatggag aacaggtcaa gatggcagaa
    36001 ggcgttcccg tctttgtttc tgctaaggaa gacaatcact ttaaggtgac cgtggagcag
    36061 ttagaagcag ctcggactga caagaccaag gttttggtgc tgaattcgcc atctaatccc
    36121 acaggtatga tttacacccg tgaggaactc ttggcaattg gaaactgggc tgtagaaaat
    36181 gatattctca tcctagcaga cgatatctat ggccgcttgg tttataatgg tcatgagttc
    36241 acaccgattt ctagcctatc ggaagcgatt cgcaagcaaa cagtggtcat caatggtgtg
    36301 tctaaaacct atgccatgac tggttggcgg attggttatg ccgttggaga agcagacatt
    36361 atcgctgcca tgtccaagat tgcaggtcaa acaacttcga atccgtcagc agtagcccaa
    36421 tatgcagcag ttgaggctct atcaggtgag caagatactg tagaaagcat gcgtcaggcc
    36481 tttgaggaac gtcttaatac tatctatccc ctccttgcag aggtgccagg atttgaagtg
    36541 gtcaaaccgc aaggggcctt ctatctcttc ccaaatgtca aaaaggccat ggagatgaaa
    36601 ggctacacgg atgtgacaga ctttacaact gctatcttag aagaagccga agtggccttg
    36661 gtaacaggag ctggatttgg agcaccagaa aatgtccgcc tcagctatgc gacagaccta
    36721 gacacgctta aagaagcagt cgaacgcttg aaagcattta tgggtagtga gaatgattga
    36781 tcattttgag attaaggtaa aggatttaca aatttcagaa ggattttata ggagttttct
    36841 cgctcctttg gactataaaa tggcttttaa gactagttct ctaattagtt ttctatcccc
    36901 gaacagccct catcctggtg gtgatttttg gctgactcag ggaacacaag atcctgttca
    36961 ttttgctttt ttagcagaaa ataaggaaga agttcaggct tgttatgagg ctggcttaga
    37021 ggcaggtggg cgagacaatg gggttcctgg ttatcgaagt gagcatccga tttattatgc
    37081 tgcttttatg attgacctgg atgggaacaa tatagaggtg gtttgccata aagaataaaa
    37141 aagaaaagga aaaataatga caaaacgtgt aacgattatt gatgtaaaag actatattgg
    37201 tcaagaagtg acgattggcg cttgggttgc caacaaatca ggaaaaggga aaatcgcttt
    37261 cttacaattg cgtgatggaa cagccttctt tcaaggtgtg gcttttaaac caaactttgt
    37321 cgaaaaattt ggtgaagaag tgggacttga gaagtttgat gttatcaaac gcttgagcca
    37381 agaaacgtct gtttatgtga caggtattgt caaagaggac gaacgttcta aatttggcta
    37441 tgagttggac atcacagaca tcgaagtgat cggtgaatct caagactacc caatcacacc
    37501 aaaagaacac ggaacagact ttttgatgga taaccgtcac ttgtggctac gctctcgtaa
    37561 gcaagtagct gtgttgcaaa tccgtaacgc tattatctat gcaacttatg agttctttga
    37621 caagaacggt tttatgaagt ttgacagccc aattctttca ggaaatgcgg cagaagattc
    37681 tacagaactc tttgaaactg actacttcgg aacgccagcc tacttgagcc aatcaggtca
    37741 actttaccta gaagcagggg ctatggctct tggtcgtgtc tttgactttg gtccagtttt
    37801 ccgtgctgaa aaatcaaaaa cacgccgtca cttgactgag ttctggatga tggatgctga
    37861 gtactcatac ttgacacatg atgagtcgct tgacttgcaa gaagcttatg tgaaagctct
    37921 tctacaaggt gttcttgacc gcgcgcctca agccttggaa accttggaac gtgatacaga
    37981 actcttgaaa cgctacattg cagagccatt caaacgtatc acttacgatc aagccattga
    38041 cctcttgcaa gagcatgaaa atgatgaaga tgctgactac gagcatcttg agcatggtga
    38101 tgactttggg tcaccacacg aaacttggat ttcaaaccac tttggtgtgc caacatttgt
    38161 catgaactat ccagcagcca tcaaggcttt ctacatgaaa ccggttcctg gaaatccaga
    38221 gcgcgtgctt tgtgcagact tgcttgctcc agaaggctat ggagaaatta tcggtgggtc
    38281 tatgcgtgag gaagattacg atgcccttgt cgctaagatg gatgaacttg gcatggatcg
    38341 tacagaatat gaattctacc ttgaccttcg taaatacggt acagttccac acggaggatt
    38401 tggtatcggt atcgaacgta tggtaacctt cgcagcagga acaaaacata tccgtgaagc
    38461 tattccattc ccacgtatgt tgcaccgtat caaaccataa gaacaggaaa acgcccatgt
    38521 ggcgtttttt cgaattctag tgtttttact tgcgtaaaaa aattttttct agtataatag
    38581 tttattgtga gcgaacctca cttacccctt gcaaagtctt ggggtcatta gaccaaaagg
    38641 aggaacatat caatggctaa atacgaaatt ctttatatca ttcgtccaaa cattgaagaa
    38701 gaagctaaaa acgctttggt agcacgtttt gactctattt tgactgacaa cggtgcaact
    38761 gttgttgaat caaaaacttg ggaaaaacgt cgtcttgcat acgaaatcca agatttccgt
    38821 gaaggacttt accacatcgt taacgttgaa gcaaatgacg atgcagctct taaagagttt
    38881 gaccgtcttt caaaaatcaa cgctgacatt cttcgtcaca tgatcgtcaa aattgacgcg
    38941 taagaaggta aactatgatt aacaatgttg tacttgtagg gcgtatgaca cgtgacgctg
    39001 agttgcgtta taccccatca aatgtagcag ttgcgacttt tactcttgca gtaaaccgta
    39061 catttaagag tcaaaatggt gaacgtgagg ctgattttat caatgtcgtt atgtggcgcc
    39121 aacaggctga aaatcttgct aactgggcta aaaaaggctc acttatcggg gtgacaggtc
    39181 gtatccagac tcgtagttac gataaccagc aaggacaacg tgtctacgtg acagaggtcg
    39241 tggctgagaa tttccaaatg ttggaaagcc gtagtgtgcg tgagggtcac acaggtggag
    39301 cttactctgc accaactgca aactattcag cacctacaaa ttcagtacca gacttttcac
    39361 gtaatgaaaa tccatttgga gcaacaaacc cattggatat ttcagatgat gatttaccat
    39421 tctaatggac aactaaactt ataaaggaga aaaaacatgg ctcaacaacg tcgtggcgga
    39481 ttcaaacgcc gtaaaaaagt tgattacatc gcagcaaaca aaattgaata tgttgattac
    39541 aaagatactg agcttcttag ccgtttcgtt tcagaacgtg ggaaaatcct tcctcgtcgt
    39601 gtaacaggaa cttcagctaa aaaccaacgt aaagtaacaa cagctatcaa acgcgctcgc
    39661 gtaatggctt tgatgccttt cgtaaacgaa gattaaagaa aagacccggt tgggtctctt
    39721 tttcatgagc ttgaaaacaa gaaagcgaat taagacccca acgggtcttt tttcacaagc
    39781 tttgaaatga gagagcgagt taggctcagt ggcttccttt ttacgagttt gaaaacaaga
    39841 gaacacatta aaagcacgat ggagcttttt cataaatatg tctcttttct catccaagaa
    39901 attaaaataa aaatgataaa ctttttgaaa ataggggatt tgcctacttt atgatataat
    39961 ggaaagagtt agattgaaag aggtagtgag atggacgcga aattaagata caaggcaaag
    40021 aagatcaaga ttgtcttttt tgatattgat gatacattgc ggaattcaaa gacagggttt
    40081 attccaacta caattcccac tgtttttaaa cagttgcgtg aaaaaggaat tttaatagga
    40141 atcgcctctg gacgtggcat ttttggtgtt gttccagaga ttcgtgatct caagcctgac
    40201 ttttttgtaa ctttgaatgg agcttatatc gaagataaaa aaggtcaggt catttatcag
    40261 catcagattg aaaagtcaga tgttgaggag tatatctctt gggctaagca agaaggaatt
    40321 gagtatggct tggttgggag tcatgatgcc aagttgtcga ctcgcaccga tatgatgagt
    40381 gaagctatca atccaattta tcccgactta gatgtagatc ccgatttcca tgaaaaagaa
    40441 gatatctatc agatgtggac ttttgaagat aagggagatg acttgcactt gcctgatagt
    40501 ctctcagaca aacttcgcat ggttcgttgg catcaacatt cgtctgatat tgtgccgatt
    40561 tcaggctcca aagcgacggg ggtggaaaag gttgtggaac accttggctt gaaaccagag
    40621 aaggtcatgg tttttggaga tggtctcaac gacttggaac tctttgatta tgctggaatc
    40681 agcgttgcca tgggaatttc tcatgataaa atcaaagaaa aagcagatta tattacaaaa
    40741 acattagaag aaaatggtat ttttgatgcc ttagaagtat ttggtatggt agaaaaagaa
    40801 ttgcattttc cacaagtaga cattgaaaca gtagaaggtc ctcttgcgac tattaagacc
    40861 aatcacggag acttacgtat caagcttttc cctgaacatg ctcctaaaac agtggctaac
    40921 tttgtatctc tttcaaaaga tggctactat gatggtgtca ttttccaccg tattatcaag
    40981 gactttatga tccaaggtgg agacccaact ggaactggta tgggtggcga gtcaatctac
    41041 ggcgaatctt ttgaggatga attctcagaa gagctttaca atatccgtgg tgctctttcc
    41101 atggcaaatg ctggtccaaa taccaacggc agccagttct ttatcgtgca aaaccaacac
    41161 ctaccttatt ctaagaaaga aattactcgt ggtggttggc cagaaccgat tgcagaaatc
    41221 tatgccaatc aaggtgggac acctcaccta gaccgccgtc acacggtttt tggtcagtta
    41281 gctgatgaag catcttacgc tgtcttggat gccattgctg ctgttgagac aggagctatg
    41341 gacaagccag ttgaagatgt tgtaattgaa actattgaaa tcgaggacta agatgaaaat
    41401 cggtgataag ctaaaggggc gtattacagg gattcagccc tacggtgcct ttgttgagtt
    41461 agagacgggt gatacggggc tgattcacat ttcagaaatt cggacaggat ttattgaaaa
    41521 tattcatgaa accttgaaag tcgatgaaga ggttcaagtt caggtagtgg atttagatga
    41581 atttacgggg aaagccagtc tttctatccg cactttggag gaagaaaagt accagtttcc
    41641 tagacggaga cgcttttcaa gcgaccgctt taactacggt tttgctccct ttcgacgaat
    41701 gctaccaatc tggactggag aagccctgca ccatttaaaa aagaagaagt agtttagagt
    41761 tttatatttt ttgtaatgtt aatatgaatt tgctataata ggaccatgga agaagaacaa
    41821 ttattaaaat caggagagcg cattaaccag ctcttttcga cagatatcaa aatcattcaa
    41881 aatagagagg tttttagcta ttcggtggat agtgttctct tatcacgatt tccacgtttt
    41941 cctaagaagg ggttgattgt ggatttctgt gctgggaatg gagcagtggg gctttttgct
    42001 agcactcgta ctcaagcaca gatattgtct gttgagattc aggagcgttt ggcggatatg
    42061 gctgaacgct ctgtccgttt gaatggatta gaggagcaaa tgcaggtcat ctgcgatgat
    42121 ttgaaaaata tgcctgctca cattcagggg agtaaggtgg atatgatttt gtgtaatcca
    42181 ccctatttca aggtggatcc ttattctaat ctgaacgaga gtgaacatta tctcttggct
    42241 cgacacgaaa tcacgactaa tttggaagaa atctgtcgta gtgcccagag tattctcaag
    42301 tctaatgggc gtttggccat ggttcatcgt cctgatcgac ttctggatat tttggatacg
    42361 ttaaaacgac ataatctagc ccctaagcgc ctgcagtttg tttatccaaa aagagaaaag
    42421 gaagccaata tgcttttgat tgaggcgatc aaggatggct caacaagtgg ctttaaggtc
    42481 ttacctcctt tgattgtcca caatgatgat ggctcttata cgcccgaaat cgaagagatt
    42541 tattatggat cataaggctt atatgtacgt tctggagtgt cgtgatggat cctactatat
    42601 aggctatacg actgatatga gaagacgcct cgctatccac aatagtggga agggagccaa
    42661 atatacacga gcacgcttgc cagtcaaact tatctatgct caaggttttg ccagtaagga
    42721 agaagccatg tcggctgaag ctcttctcaa gcgtaagaag aggccacaga aggaagaatt
    42781 tttatctgaa aatcaagata gaaatttact cagttatttt gaagaatcgt ggggagtcct
    42841 ttgactcctc ttacctttgt caaaaagcga aaaaaatgct acaataaaga aaataaacaa
    42901 aatgaggtat tatcatgtcc aagattctag tatttggtca ccaaaatcca gactcagatg
    42961 ccatcggatc atctgtagct tttgcctacc ttgcaaaaga agcttacggt ttggatacgg
    43021 aagctgttgc ccttggaact ccaaatgaag aaacagcctt tgtcttgaac tattttggtg
    43081 tggaagcacc acgtgttatc acttctgcca aagcagaggg ggcagagcaa gttatcttga
    43141 ctgaccacaa tgaattccaa caatctgtat cagatatcgc tgaagtagaa gtttacggtg
    43201 ttgtagacca ccaccgtgtg gctaactttg aaactgcaag cccactttac atgcgtttgg
    43261 agccagttgg atcagcgtct tcaatcgttt accgtatgtt caaagaacat ggtgtagctg
    43321 tgcctaaaga gattgcaggt ttgatgcttt caggtttgat ttcagatacc cttcttttga
    43381 aatcaccaac aacacaccca acagataaaa tcattgctcc tgaattggct gaattggctg
    43441 gtgtgaactt ggaagaatat ggtttggcaa tgttgaaagc tggtaccaac ttggctagca
    43501 aatctgctga agaattgatt gatatcgatg ctaagacttt tgaactcaac ggaaataatg
    43561 tccgtgttgc ccaagtgaac acagttgaca tcgctgaagt tttggaacgc caagcagaaa
    43621 ttgaagctgc aatgcaagct gccaacgaat caaacggcta ctctgacttt gtcttgatga
    43681 ttacagatat cgtcaactca aactcagaaa tcttggctct tggtgccaat atggacaagg
    43741 tcgaagcggc tttcaacttc aaacttgaaa acaatcatgc cttccttgct ggtgccgttt
    43801 cacgtaagaa acaagtggta cctcaattga ctgaaagctt taatgcgtaa gattttgggt
    43861 gtcagctcaa aatcggaaag tctagtttgc cttatatcga aaggagtttc ggctcctttt
    43921 ttctaggagt gaagtatgtt agaaaatggc gatttgattt ttgtgagaga tgggtcagac
    43981 atgggacagg ccatccagac ttccacaggt aactatagcc atgttgccat ttatttggat
    44041 gggatgattt atcatgctag tggacaggct ggtgttgtct gtcaagaacc ggcagacttc
    44101 tttgagtcca atcatttata cgacctctat gtttacccag aaatggatat ccagtcggtg
    44161 aaggaaagag cttgcaaaca tcttggagca ccctacaatg cttctttcta tccagatgca
    44221 gctggttttt actgctccca gtatatagca gaaatcctac ctatttttga aactattcct
    44281 atgaaatttg gagatgggga gcaggagatt agtgattttt ggagggagta ttacatagaa
    44341 ctaggtctgc ctgttcctct gaaccaagct ggtaccaatc ctagtcagtt ggcagcatcg
    44401 cctctgttac aatgtaaaga aaggaatctt catgattcag atttttaatc catctcgttt
    44461 gacgagacag ccatttttgg agaattgatc cgctatctgg atcagtatga ggatgtgatt
    44521 ctacgggaaa ttaaggctca atttccagat gttgcagttg ataaactcat ggaagagtat
    44581 ataaaggcag gcttgattct acgtgaaaat aagcgctatt acctcaattt tcctacgctt
    44641 gaatcacttg atagtcttga actggatcaa gagatttttg tcagagaagc tagtccggtc
    44701 tatcaagcct tgttggagca gagttttgag acggaattgc gcaatcaaat caatgcagct
    44761 attttagttg aaaagacgga ctttgcgcgc attaaaatga ccctgtccaa ttatttttac
    44821 aaggtcaaac agcagtatcc tttgacagaa aaacagcagg agctctatga cattttagga
    44881 gatgttaatc ctgagtatgc cctcaagtat atgacggctt ttttgttgaa atttctcaaa
    44941 aaagaccagc ttatgcagaa atgccgtgat atctttgtgg acagttaggt tgtcttaggc
    45001 tatattgtgc aaaatgaaga tggaaagtat gagttggcta tcgattttga taaggagagg
    45061 ttaactttct acttagcgtg atttcttgtt tctgagtaca ttgtttgact ttccttagta
    45121 ttcggtataa actatatgta accggtaaca catatcggaa taaactaaag gagacaatca
    45181 tatgtcactt gaaaacaaat tggaacaagc aacaggcgct gtcaaagaag gttttggtaa
    45241 agttactgga gacagcaaga cagaacttga aggagctgtt gaaaaaacag tttctaaggc
    45301 aaaagacgtt gtagaagacg caaaaggtgc tgtagaaggt gccgttgaag gtttgaaaaa
    45361 cgtttttact aaataatagg aaaaaatcaa gggtttcatt ttcccttgat tttttctatt
    45421 cttataaata attttctgcg acggctgtat ctcctgggta ggattctttc ttgccctgga
    45481 tgatttggta acaatcggct cccttacccg caataataac tgcatctaat tcgtgatttg
    45541 tgatagccat tgccgccttg atggcttctt ggcgatccgc aatcttctca acaggatgat
    45601 tgatgtagct actaatttca tctgcaatgg ccattgggtc ttcatagtta gggtcatcag
    45661 cagtcagaaa gacttgaatc tcagggtgtt gattgaggag gaggccaaag tccttacgac
    45721 gactttctcc cttgtttcct gttgatccca gaaccagagc aatctttccg gtttgatgag
    45781 tttcaaccac attgatgagt tttttcagac tatccccatt gtgggcatag tcgatgaata
    45841 ccttggctcc atttttctga gtgaggactt ccatacgacc aggaacgcgg gttgcagcga
    45901 tgcctttttt gatgtcctca agacttgctc cgagacggag acaagcaagt ccagcagcaa
    45961 ctgcattttc ttggttgaag ttgccaatga gttggatatc ataatctcca gcgagtttac
    46021 ccgtagctga aaatctaaag gctttggaat tctcgatttg gttatcaaat tggctaccat
    46081 agaaatcatg gtcttgatct tcaacctgtt ctttcaagac tgagaagtgg tccatgtcac
    46141 tgttaatgat gactgctcgg ctattttcca tcaagagacg cttgtggtag aaatagtctt
    46201 caaagctagg gtgttcaatc gggccgatat ggtctaggct gatatttagg aaaactccca
    46261 catcaaaggt tagaccatag acacgtttga ccagataggc ttgactggag acttccatga
    46321 tgaggtgggt acggtcattt tgcacagcct gattcatcat gtcaaagagg tcaatactct
    46381 caggggttgt caacgctgac ttaaagaaag tctctccatc aagagttgtg ttcatggtcg
    46441 acaacatagc aggtcgatgc ccttgagata agatgttata ggcgaaatag gctgctgttg
    46501 tcttaccctt agtaccagta aaggcaagga gtttgagttt ttcctgtgga ttaccataga
    46561 actccatggc aatcaaactc atggctttct ttatatcgtt cacaatgatg acagggatac
    46621 cgacttcgta gtccttttca gctatatacc aagctaagcc ttgtgttata gcagaaagaa
    46681 ggtattcttt tttaaaggca gcgccttttg caaaaaaaag agtgtcttct gttacttttc
    46741 ggctgtcgta gctgatgcta tcaaaaacaa ctttgctgta gttgtagtgg taatgacctt
    46801 ggtcaataat ttcgcgaaaa aggccatctt tctttaaaat atctaataca gtttcaattt
    46861 ttatcatact ttctattgta aaccgaaagt cgcaaattta caagtaacaa ggaaaagttt
    46921 ataatggaag ataaggagtt tttcctagtt atcaaaattg aatgaggaat ctatgtcgca
    46981 cgaaaacaat caccagcagg cccagatgtt acgggggact gcttggctaa cggctagtaa
    47041 ctttatcagt cgcctactcg gggctgttta cattatccct tggtacatct ggatgggggc
    47101 ttatgcagct aaggcaaatg gtctctttac catgggttac aatatctatg cttggttctt
    47161 gttggtttca acagcgggga ttccagttgc ggtggccaag caagttgcca agtataatac
    47221 catgcgagaa gaagagcata gctttgccct gattcggagc ttcttaggct ttatgacagg
    47281 actaggcctg gtttttgctt tagtcttgta tgtctttgct ccttggctag cagacttgtc
    47341 tggcgtgggc aaagacttga tcccaatcat gcaaagcttg gcttggggag tcttgatttt
    47401 cccgtctatg agtgttatcc gaggattttt ccaagggatg aataacctca aaccctatgc
    47461 catgagccaa attgctgagc aggtcattcg tgttatctgg atgctcctag caacctttat
    47521 cattatgaag ctcggttcag gagattatct agcagccgtt acccaatcaa cctttgctgc
    47581 ctttgtcggt atggtagcca gttttgcagt cttgatttat ttccttgccc aagaaggttc
    47641 actcaaaaga gtctttgaaa caggagataa gattaacagt aagcgtctct tggttgatac
    47701 cattaaggaa gccattcctt ttatcctgac agggtctgcc atccagctct tccagatttt
    47761 ggatcagctg acctttatca atagtatgag ctggtttacc aactacagca atgaggactt
    47821 ggttgtcatg ttttcttatt tctcagccaa tcctaataaa atcacgatga ttttgatttc
    47881 tgtaggggtt tcgattggga gtgttggttt gccacttttg acggaaaact atgtcaaggg
    47941 ggacttgaaa gcagcttctc gtctcgttca ggacagtctc accctactct ttatgttctt
    48001 gctaccagca acggttggag tggttatggt aggagaacct ctttatacgg tcttctatgg
    48061 taagccagat agtttggctc tgggcttatt tgtctttgca gttttgcagt ctattatttt
    48121 aggcttgtac atggtcttgt ctccaatgct tcaggccatg ttccgcaacc gcaaggccgt
    48181 tctctatttt atctatggtt ctattgccaa gctagtcttg caactaccta ccatcgccct
    48241 cttccacagt tatggtcctt tgatttcaac aaccattgct ctcatcattc ctaacgtctt
    48301 gatgtatcgg gatatttgta aagtaactgg tgtcaagcgt aaggtgattt tgaagcgaac
    48361 cattttaatc agtttgctga ccctagtcat gtttctgtta ataggaacca tccagtggct
    48421 gttaggattt ttcttccaac caagtggacg tttgtggagc ttcttttatg tagctcttgt
    48481 cggtgccatg gggggtggac tttatatggt tatgagtctg cgtacctatt tattagataa
    48541 ggtaatagga aaagcccaag cagatcgcct gcgagcaaaa tttaagcttt cgtaaggtag
    48601 catttataaa taaaaggaat aagttgaaca atcttttagg agaacttgaa gcttattcct
    48661 tttttagcgt aaaaatagct aaaattatat ttcaaaaata gaaaatgagg cttttttcga
    48721 ttttttggaa aaaatcattg actaaacaaa ctaaacgttg tataataaaa caaatattta
    48781 aagcaggagg ttccggaaat gaaaaagtct aagagcaaat atctaacctt ggcaggtctt
    48841 gtcctgggta caggagtttt attgagcgcg tgtggaaatt ctagcacggc gtcaaaaaca
    48901 tacaactatg tttattcaag tgatccatct agcttgaact atctagcaga aaaccgcgca
    48961 gcaacatccg atattgttgc aaatttggta gacgggttat tagaaaatga ccaatatggg
    49021 aatattattc catcattagc agaggattgg actgtttctc aggacggttt gacctatacc
    49081 tacaaacttc gtaaggatgc caagtggttt acttctgagg gagaagaata tgcgcctgta
    49141 actgcccagg attttgtgac aggtttgcaa tatgcagctg ataaaaaatc agaagccttg
    49201 tatctagtgc aggactctgt tgctggtttg gatgactata tcactggtaa aacaagcgac
    49261 ttttcaactg tcggtgtcaa ggcacttgat gaccaaacgg ttcaatatac tttggttaaa
    49321 ccagaacttt actggaattc aaaaacactt gcaacgatac tttttcctgt taatgcagat
    49381 ttcctgaaat caaaagggga tgattttggg aaggcggatc catctagtat tttgtacaat
    49441 ggacctttct tgatgaaagc acttgtctca aaatctgcta ttgaatataa gaaaaaccct
    49501 aattactggg atgctaagaa tgtctttgta gacgatgtga aattgaccta ctatgatggt
    49561 agcgaccaag aatcactgga acgtaatttt acagctggtg cttatactac ggctcgtctt
    49621 tttcctaaca gctccagcta tgaagggatt aaagaaaaat acaaaaacaa tatcatatat
    49681 agtatgcaaa attcaacttc atatttcttt aattttaacc tagataggaa gtcttacaat
    49741 tatacttcta aaacaagtga cattgaaaag aaatcgactc aggaagcagt tctcaataaa
    49801 aacttccgtc aggctatcaa ttttgctttt gacagaacat cttatggggc tcagtctgaa
    49861 gggaaagaag gtgcaacaaa gattttgcgt aacctagtgg ttcctccaaa ctttgtcagt
    49921 atcaagggaa aagactttgg tgaagttgta gcctctaaga tggtcaacta tggtaaggaa
    49981 tggcaaggta tcaactttgc ggatggtcaa gacccttact acaatcctga gaaagccaag
    50041 gctaagtttg cggaagctaa gaaagaactc gaagcaaagg gtgttcaatt cccaatccac
    50101 ttggataaga ctgtggaagt aacagataaa gtaggcatac aaggagttag ttctatcaaa
    50161 caatcaattg aatctgtttt aggttctgat aatgtagtga ttgacattca gcaattaaca
    50221 tcagatgagt ttgacagttc aggctacttt gctcaaacag ctgctcagaa agactatgat
    50281 ttatatcatg gcggttgggg acctgattat caagacccgt caacctatct cgatattttt
    50341 aatactaata gtggaggagt tctacaaaat cttggactag agcctggaga agccaatgac
    50401 aaggctaagg aagttggact ggatgtctat actcaaatgt tggaagaagc taataaagag
    50461 caagatccag ccaaacgtta tgagaaatat gctgatattc aagcttggtt gattgatagt
    50521 tctttagttc ttccaagtgt ttcgcgtggg ggaacaccat cattgagaag aaccgtacca
    50581 tttgctgctg cctatggttt aaccggtaca aaaggggttg aatcatataa atacctcaaa
    50641 gtacaagata agattgtcac aacagacgaa tatgcaaaag ccagagaaaa atggttgaaa
    50701 gaaaaagaag aatccaataa aaaagcccaa gaagaattgg caaaacatgt caaataaaat
    50761 ctaattgtag ataagtttgt gtaagaagtc tttgtgctta aaaacgcata atatcaggta
    50821 tataaaatcc tagatgttat gcgttttgtt ataggaagat ttattagatt ttatcctcaa
    50881 attgagttat tcccgacttt atccgttgaa atattataag gagtatttat gaaattaaaa
    50941 ttattaagag tagatactaa ggtgattatg gggagtttct tacttgttct gtctagtcta
    51001 cttgctttgt tgcttcccct tatcttaaag ggtttaatag atgggagttc tattgaaaat
    51061 ataggttcca aagtatttca atcgtttttg atttttattg gtcaagcctt gttttcttct
    51121 attggttact atctgtttag tcaatcgggt gaaaaaaaga tagcaaaaat caggaaaaag
    51181 tgatagaggg gttgatttat gcagagaaat ccttctttga taagagccaa agtggggagt
    51241 tgacttctgc cattgtcaat gacacgagtg tcattcgtga gtttttaatt acgactttcc
    51301 caaatattat tctgagttta gttatggtac ttggttccat tgtagtctta tttagtcttg
    51361 attggaatct ttctctactt ttattcatca ctcttccttg tatgatgttt attatcttgc
    51421 ccctttccaa tatcagtgaa aagtatagtc gtcgtttaca ggaggaaatc ggatttttaa
    51481 cagggcaatt gactgaaaag attcaagaac atgaattaat taagactaac caagcagaga
    51541 aaagtgtaca ggatgttttg gataattgta ttgaaagggt acaaaacaat tcactgaaat
    51601 cagatagggt tacttctttt gagaccccgt ttgctctctt atttatcttt gcgactatag
    51661 ctgtgatgct aacctatggg ggttatcggg tcagcgcagg atatatatct gtgggaacct
    51721 tggtttcgtt tttgatttac ctctttcaat tacttaatcc tattagtaat atagctaatt
    51781 ttgtaactgt ttattctagg agcaagggat cttcagttgc actggagaac ttgcttgcag
    51841 ttcctaaaga aaaatttgag ggaggaaaat cggtatcagg acaagggttg aattttaacc
    51901 atgtttattt tggttatgat gaaaatcgac ctgtcttaaa ggatattact tgttcaattt
    51961 tcaaggggca aaaaattgct tttgttggac catctggatc aggaaaatca acgattgtgc
    52021 gtttgttaga gcggttttat aaaccgcttt caggagatat tctaatggag caatcaagta
    52081 tatatgattt taacttaaaa gaatggagaa gtaaaatcgc ttgggtttca caaaataatg
    52141 cagtcttatc tggcagtatt cgtgacaatc tttgtctcgg tttgaatcgc ttagtaactg
    52201 atgatgaatt gatgaaagtg ctagacttag tatcactagg tgatgagatt cgctccatga
    52261 aagagggact agatactgaa gttggtgaac gcggacgatt cttgtcaggg gggcaaagcc
    52321 aaagacttca aatagctaga acctacttaa aagatgctga aattcttata tttgatgaag
    52381 ccactgctaa tcttgatgcg gattctgagt atgcgattat cagtatcctc tattctgcat
    52441 taaaggagaa gacggttgtg attatagcgc atcgtttgtc aacggtaaaa gatgtagatt
    52501 gtattttctt cttagaggag aggaaaatca ctggctcagg aactcataag gaactactgg
    52561 aaaatcatga gcgttatgct cgttttgtgc aggagcaaat gatagagtga aggtgtcttt
    52621 tgagattcac cattttatag tctattaaag ggagcaggaa aaactccctt tttatatagt
    52681 ttgaaactat aactagctct tgaaaagaag aaaatgagtt gatgaaaata agtggtacaa
    52741 tagttactat agatttggag gtattgtatg agcaaggaat tacacattaa cacaattttg
    52801 gcccaggcgg gtattaagtc agatgaagcg acaggtgcat tggtgacacc gcttcatttt
    52861 tcaacgacct atcagcatcc agagtttggt cgatctactg ggtttgacta tacgcgaact
    52921 aaaaatccaa ctcgtagtaa ggctgaggaa gtcttggcgg ctattgagtc agcagactat
    52981 gccctagcga ctagctcagg gatgtcagct attgtactgg cctttagcgt ctttccagta
    53041 ggaagtaagg tcttggcagt gcgtgatctt tacggtggtt cttttcgctg gtttaaccaa
    53101 gtggagcagg aaggtcgttt ccattttacc tatgccaata cagaagaaga gttgattgcc
    53161 gagttagaaa aggatgtgga tgttctctat atcgaaaccc caaccaatcc cttgatgttg
    53221 gaatttgata tcgaaaaact agcaaaattg gctcatgcta agggtgccaa agtggtggtg
    53281 gacaatacct tctatagccc tatctaccaa cgtccgattg aagatggagc agatatcgtt
    53341 ctccattcag caaccaagta tctagcaggc cacaatgatg tcttggctgg agtggttgtg
    53401 accaatagtt tagaactata cgagaagctt ttttacaatc tcaatacaac aggggcagtc
    53461 ttgtctccat ttgacagcta ccagttgctt cgtggtctca agaccttgtc tcttcgtatg
    53521 gagcgctcaa cagctaacgc ccaagaagtg gttgcctttt tgaaggattc tccagcagtt
    53581 aaggaagttc tctacactgg tcgtggaggc atgatttcct ttaaagtagc cgatgaaaca
    53641 cgcattcctc atattttgaa cagtctcaag gtcttctctt ttgcggaaag tttgggtgga
    53701 gtggaaagtc ttattactta tccaacgact caaactcatg ctgatattcc agcagaagta
    53761 cgccattctt atggtttgac agatgacctc ttgcgtttgt ctattgggat tgaggatgct
    53821 agagatttga ttgcagattt gcgccaagcc ttagaaggat aagacaaaga tgggaaaata
    53881 tgattttaca agcctgccca accgtttagg gcaccatacc tataaatgga aagaaacaga
    53941 aacggatagt gaagttctac cagcttggat agcggatatg gactttgtag tcttgcctga
    54001 aatccaccaa gccgtgcaaa cttacgcaga ccaactggtt tatggttata cctatgccag
    54061 tgaagactta attaaggaag ttcaaaagtg ggaagctaca caatacggtt acaactttga
    54121 caaagaggct cttgtcttta tcgagggtgt ggtaccagcc atctcaacag ctattcaaac
    54181 ctttacaaaa gaaggcgagg cggttttaat taacacgcct gtctacccac cctttgctcg
    54241 cagtgtcaag ttgaataatc gtagattgat tactaattcc ttagtggaaa aggatggtct
    54301 gtttgagatt gactttgacc aacttgaaaa ggatttggtg gaagaggagg ttaaactcta
    54361 tattctttgc aaccctcaca atcctggtgg acgtgtttgg gaaaaagaag tgttggagaa
    54421 gattggccaa ctttgccaaa aacacggtgt tttgttagtt tcggatgaga ttcaccaaga
    54481 tttgaccctc tttggtcaca aacaccagtc tttcaatacc atcaatcctg ccttcaaaaa
    54541 ttttgctatc gtcttgagca gtgccactaa aacatttaat attgctggaa caaaaaattc
    54601 ctatgcagtc attgaaaatc ctaagttgag actggctttc cagaaacgcc tgttggccaa
    54661 taatcagcat gaaatttcag gcttgggtta tttggcgaca gaagctgcct atcgatacgg
    54721 taaagattgg ctagaggaac tcaagcaagt ctttgaagac cacatcaatt atgtggtgga
    54781 tctatttgga aaagagacta aaatcaaggt catgaaaccg caaggtacct acttgatttg
    54841 gcttgacttt tcagcctatg acctgactga tgaaacattg caagagttgt tgagaaatga
    54901 agccaaggtt atcctcaacc gtggtttgga ttttggagag gaaggaagtc tccatgcccg
    54961 catcaatata gctatgccca aatctctgtt gcaagaagtc tgtcagcgga ttgtggctac
    55021 ttttgccaaa cgttaaaaat
//
LOCUS       DAVBGY010000007        48678 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVBGY010000007 DAVBGY010000000
VERSION     DAVBGY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48678
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.007"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(46..1935)
                     /locus_tag="VQ620_000426"
     CDS             complement(46..1935)
                     /locus_tag="VQ620_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET2763109.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWSRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKTLQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2073..3638
                     /locus_tag="VQ620_000427"
     CDS             2073..3638
                     /locus_tag="VQ620_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET2763110.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIALPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3638..4378
                     /locus_tag="VQ620_000428"
     CDS             3638..4378
                     /locus_tag="VQ620_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2763111.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(4487..4702)
                     /locus_tag="VQ620_000429"
     CDS             complement(4487..4702)
                     /locus_tag="VQ620_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763112.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(5573..6823)
                     /gene="ilvA"
                     /locus_tag="VQ620_000430"
     CDS             complement(5573..6823)
                     /gene="ilvA"
                     /locus_tag="VQ620_000430"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HET2763113.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(6873..7229)
                     /locus_tag="VQ620_000431"
     CDS             complement(6873..7229)
                     /locus_tag="VQ620_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET2763114.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEIFGDE"
     gene            complement(7467..7703)
                     /locus_tag="VQ620_000432"
     CDS             complement(7467..7703)
                     /locus_tag="VQ620_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763115.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            complement(7810..8832)
                     /gene="ilvC"
                     /locus_tag="VQ620_000433"
     CDS             complement(7810..8832)
                     /gene="ilvC"
                     /locus_tag="VQ620_000433"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET2763116.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(8898..9374)
                     /gene="ilvN"
                     /locus_tag="VQ620_000434"
     CDS             complement(8898..9374)
                     /gene="ilvN"
                     /locus_tag="VQ620_000434"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET2763117.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9367..11067)
                     /locus_tag="VQ620_000435"
     CDS             complement(9367..11067)
                     /locus_tag="VQ620_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET2763118.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDILPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(11267..12934)
                     /locus_tag="VQ620_000436"
     CDS             complement(11267..12934)
                     /locus_tag="VQ620_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET2763119.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(12937..13302)
                     /locus_tag="VQ620_000437"
     CDS             complement(12937..13302)
                     /locus_tag="VQ620_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2763120.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(13459..13647)
                     /locus_tag="VQ620_000438"
     CDS             complement(13459..13647)
                     /locus_tag="VQ620_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET2763121.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(13763..13939)
                     /locus_tag="VQ620_000439"
     CDS             complement(13763..13939)
                     /locus_tag="VQ620_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET2763122.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            complement(14050..14199)
                     /locus_tag="VQ620_000440"
     CDS             complement(14050..14199)
                     /locus_tag="VQ620_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET2763123.1"
                     /translation="MDKVETTIKSLCVHHYNLKFYVFNDDLPREWFQLMEKRLETLNS
                     EIVNV"
     gene            complement(14511..16055)
                     /locus_tag="VQ620_000441"
     CDS             complement(14511..16055)
                     /locus_tag="VQ620_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET2763124.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            16268..16570
                     /gene="gatC"
                     /locus_tag="VQ620_000442"
     CDS             16268..16570
                     /gene="gatC"
                     /locus_tag="VQ620_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET2763125.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTNRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            16570..18036
                     /gene="gatA"
                     /locus_tag="VQ620_000443"
     CDS             16570..18036
                     /gene="gatA"
                     /locus_tag="VQ620_000443"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET2763126.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPATFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            18036..19478
                     /gene="gatB"
                     /locus_tag="VQ620_000444"
     CDS             18036..19478
                     /gene="gatB"
                     /locus_tag="VQ620_000444"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET2763127.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            19607..20167
                     /gene="efp"
                     /locus_tag="VQ620_000445"
     CDS             19607..20167
                     /gene="efp"
                     /locus_tag="VQ620_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET2763128.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            20189..20578
                     /locus_tag="VQ620_000446"
     CDS             20189..20578
                     /locus_tag="VQ620_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2763129.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            20571..20993
                     /gene="nusB"
                     /locus_tag="VQ620_000447"
     CDS             20571..20993
                     /gene="nusB"
                     /locus_tag="VQ620_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET2763130.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDCEDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(21042..21269)
                     /locus_tag="VQ620_000448"
     CDS             complement(21042..21269)
                     /locus_tag="VQ620_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2763131.1"
                     /translation="MLSGLIFGLSHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRL
                     TILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(21291..21742)
                     /locus_tag="VQ620_000449"
                     /pseudo
     CDS             complement(21291..21742)
                     /locus_tag="VQ620_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21760..21945)
                     /gene="briC"
                     /locus_tag="VQ620_000450"
     CDS             complement(21760..21945)
                     /gene="briC"
                     /locus_tag="VQ620_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HET2763132.1"
                     /translation="MTGTETFTVISTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            complement(22104..22871)
                     /locus_tag="VQ620_000451"
     CDS             complement(22104..22871)
                     /locus_tag="VQ620_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HET2763133.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(22868..23734)
                     /locus_tag="VQ620_000452"
     CDS             complement(22868..23734)
                     /locus_tag="VQ620_000452"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HET2763134.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(23771..25138)
                     /gene="accC"
                     /locus_tag="VQ620_000453"
     CDS             complement(23771..25138)
                     /gene="accC"
                     /locus_tag="VQ620_000453"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET2763135.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(25150..25572)
                     /gene="fabZ"
                     /locus_tag="VQ620_000454"
     CDS             complement(25150..25572)
                     /gene="fabZ"
                     /locus_tag="VQ620_000454"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET2763136.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(25569..26054)
                     /locus_tag="VQ620_000455"
     CDS             complement(25569..26054)
                     /locus_tag="VQ620_000455"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET2763137.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(26057..27292)
                     /gene="fabF"
                     /locus_tag="VQ620_000456"
     CDS             complement(26057..27292)
                     /gene="fabF"
                     /locus_tag="VQ620_000456"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET2763138.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(27314..28045)
                     /gene="fabG"
                     /locus_tag="VQ620_000457"
     CDS             complement(27314..28045)
                     /gene="fabG"
                     /locus_tag="VQ620_000457"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET2763139.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(28079..28999)
                     /gene="fabD"
                     /locus_tag="VQ620_000458"
     CDS             complement(28079..28999)
                     /gene="fabD"
                     /locus_tag="VQ620_000458"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET2763140.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(28992..29966)
                     /gene="fabK"
                     /locus_tag="VQ620_000459"
     CDS             complement(28992..29966)
                     /gene="fabK"
                     /locus_tag="VQ620_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HET2763141.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            complement(30085..30309)
                     /locus_tag="VQ620_000460"
     CDS             complement(30085..30309)
                     /locus_tag="VQ620_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET2763142.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(30369..31343)
                     /locus_tag="VQ620_000461"
     CDS             complement(30369..31343)
                     /locus_tag="VQ620_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HET2763143.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANNRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(31343..31777)
                     /locus_tag="VQ620_000462"
     CDS             complement(31343..31777)
                     /locus_tag="VQ620_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2763144.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(31871..32656)
                     /locus_tag="VQ620_000463"
     CDS             complement(31871..32656)
                     /locus_tag="VQ620_000463"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET2763145.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            33026..34390
                     /locus_tag="VQ620_000464"
     CDS             33026..34390
                     /locus_tag="VQ620_000464"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET2763146.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREIGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            34393..34764
                     /locus_tag="VQ620_000465"
     CDS             34393..34764
                     /locus_tag="VQ620_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HET2763147.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            34978..36252
                     /gene="serS"
                     /locus_tag="VQ620_000466"
     CDS             34978..36252
                     /gene="serS"
                     /locus_tag="VQ620_000466"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET2763148.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLANYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(36277..37176)
                     /locus_tag="VQ620_000467"
     CDS             complement(36277..37176)
                     /locus_tag="VQ620_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HET2763149.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(37286..37834)
                     /locus_tag="VQ620_000468"
     CDS             complement(37286..37834)
                     /locus_tag="VQ620_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET2763150.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(38052..39374)
                     /locus_tag="VQ620_000469"
     CDS             complement(38052..39374)
                     /locus_tag="VQ620_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HET2763151.1"
                     /translation="MLELLKSIDDFAWGPPLLILLVGTGIYLTIRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISSAITALVLSVFVAIAAFGGLKSISKVSTTVVPFMTIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVFFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFNK"
     regulatory      complement(39465..39553)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(39724..42057)
                     /locus_tag="VQ620_000470"
     CDS             complement(39724..42057)
                     /locus_tag="VQ620_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HET2763152.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLVPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QNFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDIFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFF"
     gene            complement(42161..42709)
                     /locus_tag="VQ620_000471"
     CDS             complement(42161..42709)
                     /locus_tag="VQ620_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET2763153.1"
                     /translation="MISLLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPVTTSWLKQIWVTNLIG"
     gene            complement(42706..43008)
                     /gene="zapA"
                     /locus_tag="VQ620_000472"
     CDS             complement(42706..43008)
                     /gene="zapA"
                     /locus_tag="VQ620_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET2763154.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            43095..43976
                     /locus_tag="VQ620_000473"
     CDS             43095..43976
                     /locus_tag="VQ620_000473"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HET2763155.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVTLHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQETNRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            43980..44594
                     /gene="lepB"
                     /locus_tag="VQ620_000474"
     CDS             43980..44594
                     /gene="lepB"
                     /locus_tag="VQ620_000474"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET2763156.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            44674..44796
                     /locus_tag="VQ620_000475"
     CDS             44674..44796
                     /locus_tag="VQ620_000475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase"
                     /protein_id="HET2763157.1"
                     /translation="MKIKEQTRKLAVGCLKQCFEVVDRTDEVSSKYCFEVADGS"
     gene            44873..47239
                     /locus_tag="VQ620_000476"
     CDS             44873..47239
                     /locus_tag="VQ620_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HET2763158.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLIRILEKGKQKSQDLETIQKHITTVEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNSIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(47287..48570)
                     /locus_tag="VQ620_000477"
     CDS             complement(47287..48570)
                     /locus_tag="VQ620_000477"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET2763159.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFENQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT        14272 a        11162 c         9175 g        14069 t
ORIGIN      
        1 ctatcttcca ttatatcata ctttttcagt ctatggtagc tctctttaaa aggctccgat
       61 actgccgcca cctccgcctc cagagaagcc accgccagaa cttccacttc cagaagatac
      121 ggagtaggta cttgctgtat ttgcgacact agcataatgg ctcatttgcg cgcttgaatg
      181 ataaaacata ctgtgccagc cataagctac atagagattg atatctggat tttcaacttg
      241 aatctgatga accttcatca aatgactaac cttgtccgca tagccaaata aggttgcata
      301 gaccaagaga cgattccaaa caacaatact ttccaattca gccttatcca gtcgtgcaat
      361 ctcacgtaac atattttcaa aactggtcca gagatagtag acctcagctc ccgcttcatt
      421 tagaacacca tcacgattat ctagtcgaag cttccaataa tagaaaacag acaaaactaa
      481 ccctagaaaa ccaagtattg gcaaagggag gtaaagatag ccatgaacgt ctaaactgta
      541 caagaacaaa ccaaatccga taaatagggg caagatagtc aagacaccca tacccacttg
      601 caaagtcttt tccaaaccag ttaaaggacg atagtaatct gggagccccc agaaggaaac
      661 tcgttttctc actccttctt gcatctggtt caatacctct tcaaaagaag atttgagttg
      721 aagccctctt gcttgaatcc gtttttcatc agaaactttg gctctacgat aaagactatc
      781 agataccttg taatccgcaa acaaattgga aagagtggct tcttttttgc ctgaaaaggc
      841 tagatttaga cagtctctct caaatcttga caaaccatct tctttcacca atctcaagcc
      901 aactgcatct ccttctgaaa taatagagac attcccacgg tctatcacat ctagcaaggt
      961 agcttgaata agttgatcaa aggtgaattt tccagctccc ttgaccaagg gactcacttc
     1021 ctccaaggag gtcgagtaga ctgcttctga taaaaccata ggctctaatt ccattggtgg
     1081 ttcatagaga cgatgatttt tggcatattt gactgaagga gtggtctttc ttctataaat
     1141 aaaatagaag cagacactca ataacaagga gatagaaagt atcgaaggga ggacccaagt
     1201 aacgagttgt ttactctgat ctttttctct aacaatcgag tcttctatct tattaaactc
     1261 ttctaaacga ttccctttca agccctgatc cctagcgcta gcaaaatcgg tccgagacca
     1321 ataggcatgc aactcaactc cacgcttagc cggaagattg tctaaacgga tagtataatc
     1381 aaggttactc ttttcaatcg ttccctctct aaaaagtttc cctgtatgga aaaagagttt
     1441 ttcagccccc ttgtctcccc ttacatgaaa ttcaaacttt tcaatagact ctgaactatc
     1501 tgtcagaggt tgccaattta attcagcgat atcatcataa aggaaaagta aattttttaa
     1561 gttccagacg aggtcaactt caactatgtc gccctcctga cctggattat agactctcac
     1621 agtataacca tccgcttctt ctgttacttc gctagtcaca tctgctagtt ctgcaccgtt
     1681 tttcgcagcc tgaacctttg gatgagggtc aatgtcaaac ccgctaggca tcttaccagc
     1741 acgtccaagt cccacgattt ggcccttaaa gtcctcctca aactggtaaa ctatcttctg
     1801 tctaaactct gccgtattat ctgcatgaat atacaaatcc ccttgataag agtttatctt
     1861 gaaatcaatg gcaaaaacag agagtggaag aaggcaaaac aagcctaaca ccagtaagaa
     1921 aaaagttttt ttcatcaact aagccccctt tttatctttc ttatcattat agcatctttt
     1981 cgtaagtaaa ggcttactta tgttgaaaaa ttacgcattt ttcgataaaa taggagacag
     2041 ttatttttta atagatcaga ataggagaaa tcatgagaaa aatatactta tctattttca
     2101 caagtctctt gctgatgcta ggacttgtca atgttgctca agccgatgaa tatttacgca
     2161 tcgggatgga agcagcatat gctcccttta actggaccca ggatgatgat agcaacggag
     2221 ctgtcaaaat cgatgggacc aatcagtatg ccaacggata cgatgttcaa atcgccaaga
     2281 aaatcgctaa ggacttaggt aaagaacctt tggttgttaa aaccaagtgg gaaggtctag
     2341 tccctgccct tacttctggt aagattgaca tgattatcgc aggtatgagt ccaactgcag
     2401 aacgcaaaca agaaattgcc ttttcgagca gttactacac tagcgaacca gttttgcttg
     2461 tcaaaaaaga ttctgcctac gcaagtgcta aatctttgga tgactttaat ggtgcgaaaa
     2521 tcacttctca acaaggtgtc tacctctacg acttgattgc acaaatccca ggtgctaaaa
     2581 aagaaacagc catgggagac ttcgctcaaa tgcgccaagc tcttgaggct ggtgtcattg
     2641 atgcttatgt ttctgaacgt ccagaagcac tgactgctga agctgcgaac tctaagttca
     2701 agatgattca agtagaacct ggtttcaaaa ctggggaaga agatacagct atcgctatcg
     2761 ggcttcgtaa aaatgacaat cgtattagcc aaatcaatgc cagcattgaa accatttcaa
     2821 aagatgacca agttgccttg atggatcgta tgatcaagga acaacctgcc gaagctacaa
     2881 caactgaaga gactagcagt agtttcttta gccaagtcgc taaaattctt tctgaaaact
     2941 ggcaacaact cttgcgtggt gctggtatca ctcttttaat ctctatcgtc ggaaccatca
     3001 taggtctcat tattggactt gccattggtg tcttccgtac tgctcctctc tctgaaaaca
     3061 aagccattta tggccttcaa aaactagtcg gctgggttct caatgtctac attgaaattt
     3121 tccgtggtac gccaatgatt gttcaatcga tggttatcta ctatggaact gcccaagctt
     3181 tcgggatcaa ccttgaccgt acactggctg ctatcttcat cgtttcaatc aataccggtg
     3241 cctacatgac tgaaatcgtc cgtggtggta tcctagcagt tgacaaggga caatttgaag
     3301 ctgcgactgc tcttggtatg acccataacc agaccatgcg taagattgcc ctacctcagg
     3361 tagtccgtaa catcctacct gcaactggta atgaatttgt catcaatatc aaagatacat
     3421 ctgtattgaa cgttatctct gttgtcgaac tttatttctc aggaaatacc gtggcaacac
     3481 aaacctatca atacttccag acatttacaa tcatcgccgt gatttacttt gtcctcacct
     3541 tcaccgtaac acgtatccta cgatttatcg agcgccgaat ggacatggat acctatacta
     3601 caggtgctaa ccaaatgcaa acggaggatt tgaaataatg acacaagcaa tccttgaaat
     3661 taaacacctc aaaaaatcct atggacaaaa cgaagtgcta aaagacattt cactcactgt
     3721 ccacaaggga gaggtcatct ctatcatcgg aagctctgga agcggaaaat cgaccttcct
     3781 acgctccatt aacctacttg aaacaccaac tgatggacaa atcctttatc atggacaaaa
     3841 cgtcctcgaa aaaggctatg acctcacgca ataccgtgaa aagttgggga tggttttcca
     3901 atcctttaac ctctttgaaa atctcaatgt tcttgaaaac acaatcgtcg ctcagacaac
     3961 tgtcctaaaa cgcgaacgca cagaagctga aaagattgcc aaagaaaacc tggaaaaggt
     4021 cggcatggga gaacgctact ggcaagccaa accaaaacaa ctctcaggtg gtcaaaaaca
     4081 acgtgtggcc atcgctcgtg ccctctccat gaatccggac gctattctct ttgatgaacc
     4141 aacatcagct ctcgatccag aaatggttgg agaagtcctc aaaatcatgc aggacctggc
     4201 tcaggaaggc ttgaccatga ttgtcgtaac ccatgaaatg gaatttgccc gtgatgtctc
     4261 tcaccgtgtt atctttatgg ataagggcgt gatcgctgaa gaaggtaaac cagaagacct
     4321 cttcaccaat cctaaagaag accgaacaaa agagttcctt caacgctatc tcaaataaaa
     4381 agaaaaggct gcatcaaccg tgcagtcttt ttgctgtcct caaaatgaaa aggcagattc
     4441 tctatctcta ctacttgaag gtcaagaatc cgccgttatc caaagattaa tcttcaaatt
     4501 tttcataatt tttttcatag aaatcaatta agcctagggc tgtttcaaat gaaatatttt
     4561 ttacttttgc acgaccctgc gccagagcaa tgatagacat ttcacgcgca ttcgtttctt
     4621 tagagatacg gtagcctgtg attttcttat cacgaaccca gccaacaact gattctactt
     4681 tttcaaagtt tgacttagcc atgtttttct cctattttct tctttataat atttaattgt
     4741 atacgttttt actacttttg tcaataaaaa aaacatatat tcaacgaaaa taattagttc
     4801 agaattttct tacttccttt ttaaatccga taatatatag gttttgctag cctctacacc
     4861 ataaatattt gctaataaaa ctactgtatt attaccctct taaggtgcga aattattcaa
     4921 gttcttaaaa aatgcttctc tatttccgta agatagctta tttcttcgat ttaagaactg
     4981 aagatgatag ttctaacctt tagaaatcca agccttcact agctcaaaaa ctcctatctt
     5041 tcttcctcaa taaatcatct gagagatagg tattccttat atccttcaaa ttcatcactc
     5101 ctttggatat tgaggtgcta gcctagaagc agacacccga ttatctaaac catccttaat
     5161 agagatgaca agacagataa gctcagttaa ttacttatat atctttttaa tatggtatat
     5221 ttgtcaacaa aaagaaatca aatcatacct tatactcaat gaaaatcaaa gagcaaacta
     5281 ggaagctagc cgcaagttgc tcaaaacact gttttgaggt tgcagataga gctaacgtgg
     5341 tttgaagaga ttttcgaaga gtattaaaga cagtgtgact atgcttgtca atagatagga
     5401 aatcaaaatg aaggatggaa atctgagcca aacatcataa gtgctataat gaacagaagt
     5461 aagaattgca cgataagttt tctaaaacca taggaatcgt cccctttctt catcttcatt
     5521 atagcatata tacatcagtt gtacaaataa tatgatattt ttttattagt cctcagacaa
     5581 gcatattata aagcgtttca ttaccattta agttaatata agctggatca aaaccttcca
     5641 ttctacgaat caaacctgca taatcatgct tatctgctaa agcgatccca atcaatactg
     5701 ggcctgttcc cttgctagct cgtttgatat actcaaaacg tgtgatatca tcatttggcc
     5761 ccaggatatc atttacaaac tcacgcaaag ctcctggacg ttgtgggaaa ttgaccacaa
     5821 aataatgttt gataccatca taaatcaagg cacgctcttc catttctggc atacggttga
     5881 tatcattatt tcctccagaa atgatacaac aaatggtttt ccccttaata tattcagcta
     5941 aaacctctaa agaggcgata ctagctgctc cagcaggttc tgcgactatc ccttgcttag
     6001 agtaaaggtc aatcaaggtt tcagaaatca atccctcatc gacacctacc aaagtttgaa
     6061 tatgttgacg agttgcttca taggtcaact gacctacctt ttgcacagca atcccatccg
     6121 caaatttatc aatttccttg agtttgactg gacctccagc ctcaaaggca gctttcatgg
     6181 aacgcgctcc attcgcctct actccgataa cctcaatctc tggacttgtt tccttgatat
     6241 aggtagaaac cccggcaatg agaccgccac caccaacagg aaccaagaca gcatcaaaat
     6301 caatcgattc ttttcgagct tcttctaaaa tctcataagc aactgttcct tgacctgctt
     6361 gaacatgagc atcatcaaaa ggatcaataa aggtacgatt ttcagagact gtaaattctt
     6421 gagctgcttt ggctgaggca tcaaaggtat ctccaactag tttaatagtt acaaaatccc
     6481 caccaaaaaa gcgaacctga ccaatctttt gttgtggcgt agtaataggc ataaagatag
     6541 tagcaggaat tttcatttca ttacaagtat aggctactcc ctgcgcatga tttcccgcag
     6601 aagcgcaaac taccccacgt tcacgttctt ccttgctgag ctgggaaatg gcataatagg
     6661 caccacgaat tttaaaggag cgaacacgct gggcattttc ttttttcaaa taaatcttag
     6721 caccatactt ctccgataaa taatgatcgt aatccagtgg agtattcaca accacaccgt
     6781 tcaagacctt gtgagccttg attatatctt ttgaacttaa catataaaac ctctttttct
     6841 ataaaaaaca gtcactaatg actgtttttt agttattcat ctccgaaaat ttcagtaaca
     6901 ctttcatggt atttatgagc catacgaatc gaaaactctt taggaagttc ataacgattt
     6961 gtggaaatcc aatcatataa ccattctacc aaactttctt cactatcatt acgatggaag
     7021 ataaaatagt ttaaaatttc tttatattct acatcattca aatccaacgt gtctacaaat
     7081 tgactacgag gaacaaaatt agaatctaat tttcttagtt gatctgtcaa ttctcccaat
     7141 tgtaaatcca actgaaaaaa tagtttttgc agttgcctat cagtttcatc acatcctgtc
     7201 ttgatagaaa acaaattggt aatattcata tgatttcaac ccctaaataa gtacatctaa
     7261 tatcccacga atagctgaag ctaaagtttt cgcagtccag tcatttaaac ccgattcttt
     7321 caatccagat gcaataattt gctctatttg atataaaccg ccatctatcg tctcatttat
     7381 tctgcgaaga ggaccttcaa tataggataa agcttctccg aatctaacag ctgatttagg
     7441 tcctaatgat ttaccaacta tatttgttaa tattttttat ttctcaaaat ccatttcgct
     7501 atataacgag cagatttagt cgcaccagct ctctcaaacg gagaatgaat ttcaagacct
     7561 tcttctctag ctatttgtgc caatttgaag aattgttcaa aggtcatatg caacctacca
     7621 tcttcaaaat tagcatcatc ataaatgtta attacagcat tttcatcata cgaccaatct
     7681 gctttatttt ggtctgctag cactattacc gacggtgtaa ttacattata caactagaac
     7741 caataataac gatgcaattt ttctcataat ctttttcata aaatcatctc cagcgctata
     7801 tatttctaat tagttataga ttttgaatgc atcatcgtcg tttttaccaa cgaatggcat
     7861 tgctttacgc aattctgcac caactttttc aatttcaagg ttagctgctt gttcacggta
     7921 agcagtcaat tttggacgtc cagctttata gtcatttaca aagtcatttg caaatttacc
     7981 attttggatg tctgccaaga cagccttcat attttcttta acttgttcag tgattacacg
     8041 tggacctgat acatagtcac cgtattcagc agtgtttgaa atagattgac gcattttctt
     8101 gaatccacct tcgtagatca agtcaacgat caatttcatt tcgtgaagaa cttcaaagta
     8161 agccaattct ggagcgtaac ctgcttctgt caagacttcg aaacctgctt cgataagggc
     8221 agtcaaacca ccacaaagta cagcttgttc accaaacaaa tcttcttcag tttcttcttt
     8281 gtaagttgtt tcaagaagac ctacacgagc cgctccaaca cctttacacc agtccatagc
     8341 aatgttttta gcatttcctg ttgcatcttg gtatactgca taaagagctg gaacaccaaa
     8401 tccttcttcg taagtacgac gtaccaagtg tcctggtcct ttaggagcac acatgaagac
     8461 atctacatcc gcaggaactt tgataaattc aaagtggatg ttgaaaccat gggcaaatcc
     8521 aactgcgttt ccagcttcca agtttggagc gatttctgct tcgtacaatt cttgttgaat
     8581 ttcgtctggc gccaagatca tgataacatc agccaactta gtagcttctg ctactgtgta
     8641 agtatcaaat ccatcttctt ttgctttatc aaaagattta cctggacgta caccgataat
     8701 aacgtcacga cctgaatcac gcaagttttg agcatgcgca tgcccttgtg aaccataacc
     8761 gataacggcg atttttttac cgtcaagtgc tgctacttta acatcttttt catattccat
     8821 ttgaactgtc atagcttttc tctcttttct attatttatt gccttttagg ctggtttaac
     8881 aaatttaagt tggattttta atcgcgggta aatccagttg cacccgttcg agcaatattg
     8941 cgaataccgt atgggcgaat gactctcaat agggcttcgc tcttttctgc atttcccgtc
     9001 atctgaatgg taatcgagct tggcgctacg tctaccactg ttgcacggaa aggttgaata
     9061 atcgctaaaa tctcagctct cttctcagct ggcgctgaca tcttaaccaa aatcacctcg
     9121 cgctccaaat gaggcttgtc tgtaatatct cgaatgcgaa tcacatcaat ctgacgattg
     9181 agctgtttga tgatttgctc cacttcatca tgagaagcaa catcaataat aatagtgata
     9241 cgcgatacat tcggatcttc tgttgctcca acagagatgc tttcaatatt aacctgacga
     9301 cgagatagga cacctgtaaa gcgattgagg actcctgaac gattttgtag ttttgctgtt
     9361 aacattctac gcatggaact gcacccccaa catctcatga ttactcttac cagccggtac
     9421 cattggtaac acctgttcct tacgagaaat atctacctca attagcatag gaacatcctc
     9481 agtgatgact tcaaggtctt gagccaaggt ctcaggattg tcaaacttat agtttttaat
     9541 accataagcc tgcgccatca attggaaatc aggaaggata tcaaagaccg actctgatgt
     9601 tctgccttca tagaaggatt cctgccactg gcgaaccatt ccgagtgaat gattgttcag
     9661 cataaccacc ttgattggca ccttgtaaat attcaaaata gccaactcct ggttagtcat
     9721 ttggaaacca ccatccccaa caaacaagac tacttcctta tctgggttag caattttagc
     9781 accgattgct gctggaattc caaagcccat tgttcccaaa ccacctgaag tcactaactg
     9841 acgttcattt tggtagggat aatactgagc tgtccacatt tggtgttgac caacgtctgt
     9901 taccacaatg gcatctccat tcgtcaattc accaattcgt tcaataactg cttgcggttg
     9961 aaccacacgc tctttcttgt cataagaacg aacacgattc ttgtctttag tgactttctc
    10021 aatccacttt tcagtattgt tgtgaactgt tggttctgct agcaacattt gcaaggcctt
    10081 cttagcatct ccaactacag gaatgtctgc actgataatc ttgccaatct cagctgggtc
    10141 aatatcaatg tgggcaacct tagcattctt agcgaaagtc ttaggattcc ccgtcaaacg
    10201 gtcatcgaaa cgagaaccaa tactaatcat aaagtccgct tccgtcatgg caatatttgc
    10261 tgcgaatgac ccgtgcatgc ctcccattcc aagaaagagt gggtgactcg ttgcaatcgt
    10321 tccttgtccc aaaagactgg ttaccactgg aatttgatag cgttctgcaa attcatttag
    10381 ttccgtagca gcctcagcat aactaattcc accaccagct aacaagactg gctttttagc
    10441 cttggacaat tgcttcaaga ttttcttgat ttgcatatca ttcggctcaa gagtcggctg
    10501 ataacttggt aaattcactt ctggtgaata aatgaagtct gtttctaaag cagatatgtc
    10561 ttttggtagg tcaattacaa ctggccctgg acggcctgta gttgcgatat ggacagcttc
    10621 cgtaatgata cgcggaatat cagctgtctc acgaacttgg taattgtact tagtgattgg
    10681 catggtaatt cccacgatgt ctgcctcctg aaaggcatcc ttcccaatcc ctgctcgcgc
    10741 cacctgacct gtaaagacca aaaggggaac gctatcgctc atggcatccg caatccctgt
    10801 aatggcattt gttgctcctg gtccactagt gacgacggca acacccaact ttccagttga
    10861 tttggcataa ccttcagctt catgcaaaca accttgctca tggcgcccta gaatgtggcg
    10921 aatgccttta aaattatata tcgcatcata aaaaggcaaa accgcaccac caggataacc
    10981 aaagatggta tcaactccta aatcacgaag tgtttccaaa actaggtccg accccgtctt
    11041 aggagattct aaactgattt tctccattat tcccctttct ttcctcttaa aaataacttg
    11101 ttactatcat accatttttt caaaattttt caagataaaa gaagaaattt tctgaatttt
    11161 ctattttaac gtttatttat gaatgttatt tttgttttta ttaaaaagat accgatttca
    11221 tttactaaaa agaaaaaaga actgatttct cagtccttca ttaatcttat tccacactaa
    11281 ataggtatgg gtaaacaggt tgttgacctt ggtgaatctc gacttcaacg tcttcgaatt
    11341 cttctacgat ttcttgagcg atttcattgg caagttcttc gcttccgtct tcaccgacat
    11401 agaaggttac gatttcacta tcttcatcca acatatgttt caaggtttca gtcaatgttt
    11461 ggtgcatatc agggtttgac acaagaattt ttccatccac catacctaga ttatcgtttt
    11521 catggatttc taagccatcg atcgttgtat cacgcacggc tgttgtgacg cttccgctaa
    11581 cgacatcgct aagagcagct gtcatacgct cttggttttc ttcaatggac ttgcttggat
    11641 caaaggcaag aagacttgtc ataccttgag gaagagtgcg agcctctacc actactgctg
    11701 gttgctccaa aacttctgcc gcagattgag ctgccatgaa gatgttcttg ttgtttggca
    11761 agaagatgat gttacgggca ttgacctgtt caacagcctt gataaagtct tctgttgaag
    11821 ggttcatggt ttgaccgcct tcgataacat aatccacgcc ttgagaacag aagatatctg
    11881 ctagaccttt accagccacc acagcaatca aagcatactc tttttcttca gccgacttga
    11941 taacttgagt agcttctttc tcaacctgtg cttcgtgttg gttacgcata ttgtcaactt
    12001 ttaccttgac caagctacca tatttgagac cttcttgcat aacaagtcct ggatcttctg
    12061 tatgaacatg gactttgaca atttcatcat cgttaacaac gaggagagaa tccccaagct
    12121 catccaagta gttacggaat tcatcgtagt caaaatcttt agcataggtt ggaccttgct
    12181 taagagctac catgatttca gtacagtaac caaacgtgat gtcctcagtt gctacgtgac
    12241 cagctacaga cttatgatgc tctacattga tcatctcact catgttggca ggagtcgcta
    12301 caaagtcctc agatgcaata tattcgccag taagggctga aaggaaacct tcgtagatga
    12361 agaccaatcc ttgaccacct gagtccacaa cgccaacttc tttcaatact ggaagcatgt
    12421 ctggtgtttt agctagagct gttttagcac cttccaaggc tgcgcgcatg acttcaacag
    12481 tgtcatctgt ttgctcagct tttttcttag caccgatagc agctccacga gaaactgtca
    12541 aaatcgttcc ttcaacaggt ttcatcactg ccttataggc aacttccaca cctgattgga
    12601 aggccagagc caagtcttga cctgttaact cgtctttatc cttgatagct tgggaaaatc
    12661 cacggaaaag ctgagacgta atcactcctg agttcccacg cgcacccatc aaaagccctt
    12721 tggcaagaat gctcgctact tctccaactg tagaagctgg cttgtctgca acttctttag
    12781 caccattttc aatggtcatt cccatatttg tcccagtatc tccatctgga actggaaaga
    12841 cgtttaatga attgacatat tcagcttgct tattcaagcg agttgatgca gcctgcacca
    12901 tttcttgaaa taagctagta gtaatttttg acacggttat tctcctacaa ctttgatatt
    12961 ttgaatgtag acatttacag tctgagcagt aattccaagc tggttttcca agctaaaggc
    13021 aacacgctct tgaatgtttt ttgacacttc actaatcttt gttccgtagc ttaacacggt
    13081 atatacatca actgcaatac tgccatcttc ggctgccttt acgacgacac ctttagaata
    13141 attttcctta cctagcaggg cttggaaatt atctttgagg gcatttttac tagccatacc
    13201 gaccacacca aaaatctcag ttgctgctcc acctacgacg gttgcaatca cttcatctgt
    13261 tagttcgatt tgaccatctt ttgtattaat ttttacagtc atccttttta cctcaactag
    13321 ttgatactct attttatcat atttcagccc aagtgtaaaa gcagaatgat gtatcagcgg
    13381 atatttactc tatttttcaa acgatgttat acccacaata aaagaaaaaa gaccctaagg
    13441 tctcctttgc ttttattatt aaacgcgttc aactttacct gatttcaaag cacgagctga
    13501 agcccaaact tttttaggtt taccatcgat aagaacagta actttttgaa ggtttggttt
    13561 tacggcacgt tttgtttggt tcatcgcgtg tgaacggttg tttcctgata cagtcttacg
    13621 acctgtaaag taacatactt tagccattgt gttttcctcc tattagatct aatatagcgg
    13681 atgtgctagc accacatacc gtactatgtt atcacacttt cttgtttttt gcaagggaat
    13741 tggaagattt tttatttgtg tcttaaatca ggtcttgcgt gacattttct gctctccaca
    13801 tgccatcgtt gattaacaga acaccagaat taaaattatg tgtataaaaa tcatctctaa
    13861 ctgcagctaa gggtatagcc gtcaagtcca aatcccacag ctcatctatc gattttctta
    13921 caacaatatc tgaatccaaa tacagtacac gagactcgct tacatacttt ggaataaaat
    13981 acctaaaaaa gccgcatatg aaagtccctc aaaggggaga cgataacctt tcagaatatt
    14041 actgtcaatc taaacattca caatctcact attcaaagtc tctagtcttt tttccatcaa
    14101 ttggaaccat tctcgcggaa ggtcatcatt aaaaacataa aacttaagat tataatgatg
    14161 aacacaaaga gattttattg ttgtttcaac tttatccata taagcattat ctgcacctaa
    14221 gacaatcgct tttttctctt ttttctcttc tttcactttt tatctcattt ctttttattc
    14281 ccatcatatg tttctacgta accattattt tcgcctattc gttcgtaaaa ccataccagt
    14341 ggagatttta gatgaagtcc cattacggtt tacaattttt acattacgac acggagtttt
    14401 acaaatcgat ttcatttgcc aaacgtagtt agtgaggcag ttagctagtt cgccaaatag
    14461 cgactagcgt ccaacaattt ggaactttag ttccaattgt tggtactgag tcacatcttc
    14521 tcctctaact ctacgtctgg atacttgtca gcaaaccagc ggagggcaaa gtcattttca
    14581 aagagaaaga ctggttggtc aaaacggtct ttggctaaga tattgcgact tgacgacatc
    14641 cgttcatcca agtcctcagg cttgatccaa cgaacggtct ttttacccat tgggttcata
    14701 actacttctg cattgtactc gccttccatg cggtgtttaa agacttcaaa ctggagttga
    14761 cctacagcgc ctagcatgta ctcacctgtt tggtaattct tataaagctg aacggctcct
    14821 tcttgcacca attgctcaat ccccttgtgg aaggattttt gcttcataac attcttagca
    14881 gaaactttca tgaaaatttc aggagtaaag gttggcagtg gttcaaattc aaacttgttt
    14941 ttaccaactg tcaaggtatc cccaacttga taagtaccgg tatcgtaaac cccgataata
    15001 tcacctgcca cggcattgat cacattctca cgactttccg ccataaactg agtaacatta
    15061 gacagtttag cccccttacc agtacgaggg agattgacac tcatgccgcg ctcaaattcg
    15121 ccagatacga tacggacaaa ggcaatacgg tcacggtgac gagggtccat gttggcttgg
    15181 attttaaaga caaagcctga gaaatccttg tcataaggat ccacaatttc accgtctgtt
    15241 ttcttgtgac catgtggttc tggagcaaac ttgaggaagg tttcaaggaa ggtctgcaca
    15301 ccaaagtttg tcagggctga accgaaaaag acaggcgtca attctccagc cagaatagct
    15361 tcctctgaaa actcattccc ggcttcattt aaaagctcaa tatcatcctt gacttgctcg
    15421 tagaaaggat tgctaccaaa aagtttgtcc ccatcttcta gactggcaaa acgctcatcc
    15481 cctttataaa gctctaaacg ttggttatag aggtcataca agccctcaaa ggctttcccc
    15541 atcccgatag gccagttcat agggtagcta gcaatgccca agatttcttc caattcttgc
    15601 aagagatcca aaggctcacg accgtcacgg tccagcttgt tcataaaggt aaagactgga
    15661 atgccacgat gtttcacaac ctcaaacaat ttcttggttt gagcctcgat ccccttggca
    15721 gagtccacga ccatgaccgc agcatccacc gccatcaagg tacgataggt atcttctgag
    15781 aagtcctcgt gccctggcgt gtctaagata ttcacgcgct tgccgtcgta gtcaaattgc
    15841 ataacagatg aagtaacaga aatcccacgt tgcttctcga tatccatcca atcagattta
    15901 gcaaaagtcc ctgttttctt cccttttacc gtaccagcct cacgaatttc acccccaaag
    15961 tagagtaact gctcagtgat ggttgttttc cccgcgtccg ggtgggagat aatggcaaag
    16021 gtacgacgtt tcttaatttc ttcttgaata ttcataagtt ctctttcttt gattctctat
    16081 ttttcttgtt tcaatagctg agaatgattt ttacattgga ttttaccatt cctttcaaca
    16141 ctccattata tcggatttta gcattttttt caatttctat ttcttttcac ttccccctcc
    16201 cttatttata ggaaaatatg gtaaaataga acagactaaa aatcatcatt tcacgaaagg
    16261 atgcaagatg aaaattacgc aagaagaggt aacacacgtt gccaatcttt caaaattaag
    16321 attctctgaa aaagaaactg ctgcctttgc gaccaccttg tctaagattg ttgacatggt
    16381 tgaattgctg ggcgaagttg acacaactgg tgtcgcacct actacgacta tggctgaccg
    16441 caagactgta ctccgccctg atgtggccga agaaggaaca aaccgtgatc gcttgtttaa
    16501 aaacgtacct gaacaagaca actactatat caaggtgcca gctatcctag acgatggagg
    16561 agatgcctaa tgacttttaa caataaaact attgaagagt tgcacaatct ccttgtctct
    16621 aaggaaattt ctgcaacaga attgacccaa gcaacacttg aaaatatcaa gtctcgtgag
    16681 gaagccctca actcatttgt caccatcgct gaggagcaag ctcttgttca agctaaagcc
    16741 attgatgaag ctggaattga tgctgacaat gtcctttcag gaattccact tgctgttaag
    16801 gataacatct ctacagacgg tattctcaca actgctgcct caaaaatgct ctacaactat
    16861 gagccaatct ttgatgcgac agctgttgcc aatgcaaaaa ccaagggcat gattgtcgtt
    16921 ggaaagacca acatggacga atttgctatg ggtggttcag gtgaaacttc acactacgga
    16981 gcaactaaaa acgcttggga ccacagcaag gttcctggtg ggtcatcaag tggttctgcc
    17041 gcagctgtag cctcaggaca agttcgcttg tcacttggtt ctgatactgg tggttccatc
    17101 cgccaacctg ctaccttcaa cggaatcgtt ggtctcaaac caacctacgg aacagtttca
    17161 cgtttcggtc tcattgcctt tggtagctca ttagaccaga ttggaccttt tgctcctact
    17221 gttaaggaaa atgccctctt gctcaacgct attgccagcg aagatgctaa agactctact
    17281 tctgctcctg tccgcatcgc cgactttact tcaaaaatcg gccaagacat caagggtatg
    17341 aaaatcgctt tgcctaagga atacctcggt gaaggaatta acccagaggt taaggaaacc
    17401 attctaaatg ccgctaaaca ctttgaaaaa ttgggtgcta ttgtcgaaga agtcagcctt
    17461 cctcactcta aatacggagt tgccgtatac tacatcatcg cttcatcaga agcttcatca
    17521 aacttgcaac gcttcgacgg tatccgttac ggctatcgcg cagaagatgc aaccaacctt
    17581 gatgaaatct atgtaaacag ccgaagccaa ggttttggtg aagaggtaaa acgtcgtatc
    17641 atgctgggta ctttcagtct ttcatcaggt tactatgatg cctactataa aaaggctggt
    17701 caagtccgta ccctcatcat tcaagatttc gaaaaagtct tcgcggatta cgatttgatt
    17761 ttgggtccaa ctgctccaag tgttgcctat gacttggatt ctctcaacca tgacccagtt
    17821 gccatgtact tagccgacct attgaccata cctgtaaact tggcaggact gcctggaatt
    17881 tcgattcctg ctggattctc tcaaggtcta cctgtcggac tccaattgat tggtcccaag
    17941 tactctgagg aaaccattta ccaagctgct gctgcttttg aagcaacaac agactaccac
    18001 aaacaacaac ccgtgatttt tggaggtgac aactaatgaa ctttgaaaca gtcatcggac
    18061 ttgaagtcca cgtagagctc aacaccaatt caaaaatctt ctcacctact tctgcccact
    18121 tcggaaatga tcaaaatgcc aacactaacg tgattgactg gtctttccca ggagttctgc
    18181 cagttctcaa taaaggggtt gtcgatgccg gaatcaaggc tgctcttgcc ctcaacatgg
    18241 acatccacaa aaagatgcac tttgaccgca agaactactt ctatcctgat aaccccaaag
    18301 cctaccaaat ttctcaattt gatgaaccaa tcggatataa tggctggatt gaagtcgaac
    18361 tagaagacgg tacgaccaag aaaatcggta tcgaacgtgc ccacctagag gaagacgctg
    18421 gtaaaaacac ccatggtaca gatggctact cttatgttga cctcaaccgc caaggggttc
    18481 ccttgattga gattgtatct gaggcagata tgcgttctcc tgaagaagcc tatgcttatc
    18541 tgacagccct caaggaagtt atccagtacg ctggcatttc tgacgttaag atggaggaag
    18601 gttcgatgcg tgtggatgcc aacatctccc ttcgtcctta tggtcaagag aaattcggta
    18661 ccaagactga attgaagaac ctcaactcct tctcaaacgt tcgtaaaggt cttgaatacg
    18721 aagtccaacg ccaggctgaa attcttcgct caggtggtca aatccgccaa gaaacacgcc
    18781 gttacgatga agcgaataaa gcaaccatcc tcatgcgtgt caaggaaggg gctgctgact
    18841 accgctactt cccagaacca gacctacccc tctttgaaat ttctgacgag tggattgagg
    18901 aaatgcggac tgagttgcca gagtttccaa aagaacgtcg tgcgcgttat gtatctgacc
    18961 ttggtttatc agactacgat gctagtcagt tgactgctaa taaagtcact tctgacttct
    19021 ttgaaaaagc tgttgcccta ggtggtgatg ccaaacaagt ctctaactgg ctccaagggg
    19081 aagtcgctca gttcttgaat gctgaaggta aaacactgga acaaatcgaa ttgacaccag
    19141 aaaacttggt tgaaatgatt gccatcatcg aagacggtac tatttcatct aagattgcca
    19201 agaaagtctt tgtccatcta gctaaaaatg gcggtggcgc gcgtgaatac gtggaaaaag
    19261 caggtatggt tcaaatttca gatccagcta tcttgatccc aatcatccac caagtctttg
    19321 ccgataacga agctgctgtt gccgacttca agtcaggcaa acgtaacgcc gacaaagcct
    19381 ttacaggatt ccttatgaag gcaaccaaag gccaagccaa cccacaagtt gcccttaaac
    19441 tacttgcaca ggaattggcg aagttgaaag aaaactagac agaacaaaac cagccctaag
    19501 gttggttttt tcttctctac caactcccaa taactatttt ggctttattt ccagagtatt
    19561 ttatggtaaa atgaagagta ataatattta ttaaagaggt aaaaacatga ttgaagcaag
    19621 taaattaaaa gctggtatga cctttgaaac agctgacggc aaattgattc gcgttttgga
    19681 agctagtcac cacaaaccag gtaaaggaaa cacgatcatg cgtatgaaat tacgtgatgt
    19741 ccgtactggt tctacatttg acacaagcta ccgtccagag gaaaaatttg aacaagctat
    19801 tatcgagact gttccagctc aatacttgta caaaatggat gacacagcat acttcatgaa
    19861 tacagaaact tacgaccaat acgaaatccc tgtagtcaat gttgaaaacg aattgcttta
    19921 catccttgaa aactctgatg tgaaaatcca attctacgga acggaagtga tcggtgtcac
    19981 cgttcctact actgttgagt tgacagttgc tgaaactcaa ccatctatca aaggtgctac
    20041 tgttacaggt tctggtaaac cagcaacgat ggaaactgga cttgtcgtaa acgttccaga
    20101 cttcatcgaa gcaggacaaa aactcgttat caacactgca gaaggaactt acgtttctcg
    20161 tgcctaatct ctagaaagag gtcattctat gggaattgaa gaacaacttg gcgaaatcgt
    20221 tatcgcccca cgtgtacttg aaaaaatcat tgctatcgct actgcaaagg tagagggggt
    20281 tcactctttt tcaaacagat cagtgtctga taccctttca aaactttcac tcggccgtgg
    20341 catttatctt aaaaacgtgg acgaagaact cacagcagat atctatctct accttgagta
    20401 cggagtaaaa gttcctaagg tagcggttgc tatccagaaa gctgtcaaag atgccgtccg
    20461 taatatggct gatgtagaac tcgctgctat caatattcac gttgcaggta tcgtcccaga
    20521 taaaacacca aaaccagaat tgaaagatct atttgacgag gacttcctca atgactagtc
    20581 cactattaga atctagacgc caactccgta aatgcgcttt tcaagctctc atgagccttg
    20641 agttcggtac ggatgtcgaa actgcttgtc gtttcgccta tactcatgat tgtgaagata
    20701 cggatgtaca acttccagcc tttttgatag acctcgtttc tggtgttcaa gctaaaaagg
    20761 aagaactaga taagcaaatc actcagcatt taaaagcagg ttggaccatt gaacgcttaa
    20821 cgctcgtgga gagaaacctc cttcgcttgg gagtctttga aatcacttca tttgacactc
    20881 ctcagctggt tgctgttaat gaagctatcg agcttgcaaa ggacttctcc gatcaaaaat
    20941 ctgcccgttt tatcaatgga ctgctcagcc agtttgtaac agaagaacaa taaaaagaaa
    21001 aagtgtttga tatctatcaa acactttttc tttgcctccc actatctaaa aaaatgataa
    21061 tagatataat tgtaaacaaa aatccagata ggttttgcat gattgagaaa gttaaaaaaa
    21121 ctatggcaga gaatcgttaa tctcagattg tcggtagaac gataaacaag ggcaaaaaag
    21181 aaaccaatca gactataata taataaacta attggatctc tgtgagatag tatcaaatga
    21241 ctaagcccaa agataaggcc tgataagatg acatctaaat aatacttgga ctagggaaag
    21301 aaggtattca taaaataccc tctatcaaga gtctcctcaa aaacaggacc gatgattaca
    21361 ggcaggacaa aagataagat agtcgataaa aaggttggtt gtccatttga aagaaccacg
    21421 gtaaaatact catcatgaat attcctatga ttaatcaaat gagcatagcg tgcccaaaaa
    21481 ttaccgagaa tctgacaaac cacataagtt gcaaataagg agaagacaaa tgaccagttc
    21541 cagctctttt tcttttttaa cctccaaatt aatagaaaag gaaacttccc actaatccca
    21601 ctgttaaaat aagagaatag acatcagctc ctaaccctaa aatgatcgtc acatacaatc
    21661 caattgtttg tggtaaatag gtagatagta aaataataag caaaaatatt ccaaattgtc
    21721 ttagtttctt tgtgtttctc atcgtacttt tttgaaagat taccctgttc ggaagccgta
    21781 cttccaagca tctatataag aattaagtgc cccttgcctc atatagggag caaattctct
    21841 ataatataac catctactat atccatcttc ccaaacagca agaccacctg aagtttgctc
    21901 caagtcctca gttgaaataa ctgtaaatgt ttctgtacct gtcattgcaa gtaccttctt
    21961 aaaatagatt gttgtaggct cacatttata gtatatttct ttttttgtct attttatagt
    22021 ctatctcctc aactggcaat ttttcgacct gaattgcatt tttccataaa aaatgagacc
    22081 tttctagtct catttagtca ttcttagtat tttctaaatc gttgatagcg ttcttccagc
    22141 aactcttcta gcggtttttg tgaaagtcta gccagctccg tttggagttc ttttttgaca
    22201 ctcttaatta gttctttact agaaagtcct acttcagaaa tcaccttatc caccacgtcc
    22261 atttctaaca gttcatgcga agtgattttc atcagttctg ctgcttccat ggcgcgagta
    22321 ccgtccttcc ataaaatgga agcaaagcct tctggactga gaatggcata gatagaattt
    22381 tccagcatcc agacacggtc cgcgacagct agagccagag ccccgcctga accaccttca
    22441 ccgataataa tggcgataat aggaactttc aggtcactca tttccatgag attgcgagcg
    22501 atagcttccc cttgaccacg ttcttccgct ccgacaccag gataagcacc tgctgtattg
    22561 ataaaggtca caactggacg gccaaatttc tcagcctgtt tcatcaaccg cagtgccttt
    22621 cggtagcctt ctggatgtgg ttggccaaaa ttccgtttga ggttgtcttg caaactcttg
    22681 cctttttgga taccaaccac tgttacagct tgatctccaa gccaaccaat accaccaaca
    22741 actgcaccat catcacgaaa agaacggtca ccatgtaatt ggataaattc atcaaaaatg
    22801 cctgtcgcaa agtccaaggt tgtcaagcga ctctgctcac gcgcttctct gactattttt
    22861 gcaatattca tctaggactc cctccatgca atctgactag gctagcaatc gtatctggta
    22921 agtctcttct tttgacaata gcatccacaa agccatgttc taataggaat tctgcctttt
    22981 ggaaatcctc aggcaagctt tcacgaaccg tattttcaat cacacgacgc ccagcaaaac
    23041 caaccaagct ctgtggttca gccagaatga tatcaccttc catagcgaaa gaagctgtca
    23101 caccaccagt cgttggatct gtcaaaatgg tcaggtaaaa gagaccagca tttgaatggc
    23161 gtttaaccgc tgcagagatc ttagccatct gcatgagact catgattcct tcctgcatac
    23221 gggctccacc agaggctgtg aataggacaa ctggcaattt ttcgacagtc gcatactcaa
    23281 acaaacgagt gattttttca cctaccaccg tacccataga agccatgata aagttagaat
    23341 ccataatccc aagagccaca gtctgacctt taataagagc agttcctgtc acaacggctt
    23401 catgcagacc tgttttttca cgcatagatg ccagtttctt ttggtaacca gggaaatgca
    23461 agggatcctt gctttcaatc cctgtaaaca attctttgaa ggttcccata tcaatcgtca
    23521 aagccaagcg ttcttgggca gaaatacgaa aggtatagct acagtgcgga cagatacgtt
    23581 cacttcccag atccttctga tagatggtat gcttacagcc tggacactgg gaaaataatt
    23641 catctggaac ctctggctta gcttgaggtt tttccctaac cgaacgattg ggattgattc
    23701 gaatatactt atctttttta ctaaatagag ccattgattc cccttttcgg tttaaactct
    23761 taaagtcatt ttattctttt tcttgatatt taggtaagaa ggtttccatc aagaaggaag
    23821 tatcataatc cccagcaatg acattgcgat ctgaaatgag gtcaagctgg aaatctgcat
    23881 tggtctgcac tccttcaatc tctaattcat agagggcacg ttgcattttc atcaaggcgt
    23941 caaaacgatt ttcgccgtgt actatgattt tggcaatcat actatcataa taaggcggaa
    24001 tggtataacc tggataaact gctgaatcca cgcgcaagcc aactccacca cttggcagat
    24061 agagattagt aatcttacct ggacttggag caaagttaaa ggctgggttt tctgcattga
    24121 tacgacactc gatggcatga ccgcgtagga caatatcttc ttgcttaaca gacaaaggct
    24181 gacctgccgc aatgcaaatc tgttccttaa cgatatcaac acctgaaaca aactctgtta
    24241 ctggatgttc tacctgaaca cgagtattca tctccatgaa atagaaattg ctacttgctt
    24301 catcaagaag aaattcaatg gttcctgcat tctcatagcc aacaaactct gccgctcgaa
    24361 cagcagcagc acctatttca tgacgcagcg tttttccgat tgcaatcgag ggactttctt
    24421 ccaaaacctt ttggttattc ctttgaagag aacaatcccg ttcacccaag tgaatcacat
    24481 gtccatgctc atcacctagg atttgaacct caatgtgccg agctggatag ataacccgtt
    24541 ctatgtacat ggcaccattg ccataattgg ccttggcctc actagaggca gtttcaaagg
    24601 cagaaacgag gtcatctggt ttttcaacct tacgaatccc tttaccacct ccacctgctg
    24661 aagccttgag cataacagga tagccaattt tttcagcaac aatcaaagct tcttcagagt
    24721 tatgcacttc tccatctgaa cctggtataa caggcacacc tgctttaatc atctgagcac
    24781 gcgcattgat cttatccccc atcatatcca taacatgacc agatggaccg ataaacttga
    24841 tacctacttc ttcacacatg gtcgcaaatt tggaattttc actgagaaat ccaaaaccag
    24901 ggtgaatagc ttctgcctca gtcaagactg cagctgatag aactgcatta atattgagat
    24961 aagactctgt tgccttgcca ggaccaatac aaactgcttc atctgccaaa agcgtatgaa
    25021 gagcttcctt atcagcagtt gaataaaccg ctaccgtcgc aatccccaat tcacgtgccg
    25081 cacggataat acgaaccgca atttcaccac gattggcaat taaaattttt cgaaacatgg
    25141 agaacctcct tagttcccaa ttgcaaaagt aaggatacca ctggctgcaa gcttgccatc
    25201 cacttcagcc tttgcttcaa ccacagctat ggtgccacga cgttttacaa aagtcgctgt
    25261 cataaccaat tggtcgcctg gtacaacttg cttcttgaac ttaaccttgt ccataccagc
    25321 gtaaaagacc agttttcctt tattttcagg ttttgataac tccaacacac cagcagtttg
    25381 cgccaaggct tccataatca gaacacctgg cataactggg tattgaggaa agtggccgtt
    25441 aaagaaaggt tcgttaatgg tcacattttt gatagcaaca atggtatcct cgctcacttc
    25501 caagacacgg tccactagaa gcataggata acggtgggga agagcttctt tgattccttg
    25561 aatatcgatc atttgatacg taccaatcct ttaccaaact caaccatttc ttcgttagag
    25621 acgagaattt ccgttaccac accatcctta ggagctggga tttcattcat gactttcatg
    25681 gcttcgataa ttaccaatgt ttgacctttt ttgacactat caccaactgt aacgaaggca
    25741 ggtttatctg gtccagcagc caagtaaacc actccaacaa gtggactctc tacaagattt
    25801 ccctcagtag ccacacttgc ttcagctgga gctggaactt cttctgctac agtctctgct
    25861 ggagcagatg taggagctac tggactcggt gttgctagaa cgggtgctgg agcgacttga
    25921 gttgcaactt caggcacagg tctcgcttca ttcttgctaa actgcaactc atccgtccca
    25981 tttttataag aaaattctct caaacttgac tggtcaaatt gagtcatcaa gtctttaata
    26041 tcgtttaaat tcatacttat ctattctccc aacgtttgaa agcaagaact gcattgtggc
    26101 ctccaaaacc aaaagtattt gaaatagcgt atggaatttc tttctccaag ccttgtccat
    26161 aaacgacatt agcttcgata taatctgata cttcacttgt cccagctgtc attggtacaa
    26221 agttatggcg catagcttcg atggtgacga tagcttctac tgcacccgca gcccccagca
    26281 aatgtcctgt aaaagacttg gttgatgata caggtacttc cttaccaaga acagctacga
    26341 tagctccact ttctcctttt tcattggcag gagttgacgt tccgtgagca ttgacatagg
    26401 ctacttgctc tggagaaatc tcagcttctt ccaaggctag tttgatggcc ttgatagctc
    26461 cctgaccttc tggatgtgga gaagtcatgt ggtaggcatc acaagtattt ccgtaaccaa
    26521 ccacttcagc caggatagta gctccacgtt tttcagcgtg ttcaagactt tctaaaacca
    26581 acatccctga accttcaccc ataacaaacc cattgcgatc cttatcaaat gggatcgaag
    26641 cacgagttgg atcctctgta gtagagagag ctgttaaggc ttggaaacca gcgatggcaa
    26701 aaggtgtgat agaagcttct gttcctccca ccaacatcac atcttggaaa ccaaacttaa
    26761 tggagcggaa ggcatcccca atcgcatcat ttgatgaaga gcaggcagta ttgatagatt
    26821 tacaaacacc atttgcacca aaacgcatgg ctacattccc agaagccata tttggtaaag
    26881 cttttggaag agtcattggt ttgacacgtt tgggtccttt ttcatgaagg cgaagtacct
    26941 gatcttcaat ttccttgatt ccaccaatac cagatgcaac gataacacca aaacgatccc
    27001 tattaagagc ctctacatca agattggcat gatttacagc ctcttgggct gcatacaagg
    27061 catataaaga atagttatca aaacggttgg tatctttttt tacaaagtat ttatcgaacg
    27121 gaaaatcttg gatttctgcc gcattatgca catcaaagtc actatgatca aattttgtaa
    27181 tgccaccaat gccgattttc ccagttgcta aactattcca aaattcttct ggtgtatttc
    27241 cgattggaga tgttactcca taacctgtta ccactactcg atttagtttc attcttttca
    27301 cctctagctt tcgctacata cttaagccac catcaatggc aatcacttga ccagttagat
    27361 aatcttggcc tgctaaaaat actgtcaaat ctgcaacctg ctctgcctgc ccaaattctt
    27421 tcatcggaat ctgagctagt gtagcttcct taatcttatc tgataagata gctgtcatat
    27481 cagactcaat cattcctgga gcaatcacat tgactcgtat attccgacta gcgacctcgc
    27541 gtgccacaga cttggtaaag ccaatcaagc cagccttaga agcagcatag ttagcttgac
    27601 caatattccc catcaaacca acaacactag acatattaat gatagcacct tctctggctt
    27661 tcatcatcgg tttcaagact gattgtgtca tattaaaggc accagtcaga ttgaccttga
    27721 gcactttttc aaaatctgct tctgtcatct tgagcataag agtatcttgg gtaatccctg
    27781 cattgttgac caaaacatct actgaaccca gttctgcaat agcttgatca atcatacgct
    27841 tagcgtctgc aaaatctgat acatctcctg aaatgggaac caccttgata ccatagtttg
    27901 aaaactcagc gagcaattct tctgagattg ccccacgact gtttaagaca atgttggctc
    27961 ctgcttgagc aaacttgtgg gcgatggcaa gaccaattcc acgactcgaa cctgtaataa
    28021 agatattttt atgttctagt ttcatttttt tcctttcaaa acttctactt attttagtct
    28081 atttttctaa aagtgctact aaactcgctt gatcttccac atgagctaag tgagcagttt
    28141 gatcaatttt tttaacaaaa cctgacaaga ctttccccgg tccaatctcg ataaagttgc
    28201 ttatgcctgc ttcttgcatg accccaatac tttcatagaa acgaacgggt tccttgacct
    28261 gacgcgtcaa gagctgagca atgtcctctt tttgcatcac agcagcttct gtattgccga
    28321 ctaggggaca agtaaaatct gaaaaactta cctgagctag agtttcagct agtttctggc
    28381 tagctggctc aaggagagcg gtgtgaaagg gacctgacac cttaagagga atcaagcgtt
    28441 tggcacctgc ttcttgcaaa agttcaaccg ctcgatcaac tgcaaccact tctccagcaa
    28501 tgacgatttg tgcaggtgtg ttatagttgg ctggagtaac cactccaagt tcagaagctt
    28561 tttgacaggc ttcttcaatg acctctactg gcgtattgag aactgctacc atcttgccag
    28621 agtcagcagg agccgcttct tccatatagg ctccacgctt agctaccaag gcaaccgcat
    28681 cttcaaaatc caaggcgccg cttgccacca aggcagagta ttctccaaga gacaaaccag
    28741 caaccatatc aggctgatag cccttttctt gcaataaacg gtagatagca accgaagtcg
    28801 ctagaatggc tggttgcgta tagcgggtct gattgagttt gtcttcttcc gtatcgatga
    28861 gataacgcaa atcataaccg agcacctgac tcgctcgatc aatcgtttct ttaacaatcg
    28921 gatactgatc atagaaatcc cgtcccatcc ctagatactg ggcaccttga ccagcaaata
    28981 aaaaggctgt tttagtcatt tcttacaact cctgtccagc gagaggcttc ttcttgaatt
    29041 ttcttagcgg ctccgtaata caaatctttt aggatttctt cagctgtttc ttctttagaa
    29101 acaagccctg cgatttgacc tgccatgaca gagccgccat ccacatcacc gtgaacaact
    29161 gctttggcta gggcacctgc tcccatttgt tcaaagattt ctaaatcagg atcttcctgc
    29221 ttaaaggcat ctttttcagc cagttcaaaa tctctagtca actgattttt aatagcacga
    29281 acagcatgac caaagtgctg agctgaaatc gtagtatcaa tatcccttgc ttttaaaatt
    29341 ttctccttgt agtttggatg agcattcgac tcttttgcaa ctacaaaccg tgtccccacc
    29401 tgtacagcct ctgcacctag cataaagcca gccgcagcac cttcaccatc cgcaattcct
    29461 cctgcagcaa taacaggaat agatacagct gtggctacct gtcgcaccaa ggtcatggtt
    29521 gttaatttac cgatatgccc cccagcttcc attccttctg caataacagc gtctgcaccg
    29581 attttttcca tgcgtttagc taaagcgaca ctaggaacga caggaataac gattatccca
    29641 gcttcatgga aacgttccat atacttgctt ggatttcctg ctcctgttgt gacaacttta
    29701 acaccttctt caataacgag atccacgata tcttccacaa agggagataa gagcatgatg
    29761 ttgaccccaa agggtttatc agtcaatgat ttgattttat caatattagc cttgacaact
    29821 tctttcgggg catttccccc accgataatt cctaatcctc cagccttgga aacagcccct
    29881 gccaaatcac catcagcaac ccaggccatc cctccttgga aaataggata gtcaatcttc
    29941 aataattctg taatacgcgt tttcatagtg cctccaacct tccttgctta cgtaatagtt
    30001 cgatttcacc ataatttgac agtcaaacta ttacctaaac aagagggagt gggtttctcc
    30061 ctactccttc tactaatact ctgcttattt tgcttgctct tcaacgtaag caaccaagtc
    30121 accaactgtt ttcaagtcat tttctgcttc gatttggata tcaaaagcat cttcgatttc
    30181 tgagattact tggaacaagt ccaatgaatc tgcgtccaaa tcatcaaaag ttgattcaag
    30241 tgttacttct gatgcgtctt ttccaagttc ttcaacgata atttcttgta ctttttcaaa
    30301 tactgccatg ataggactcc tttaaaataa atagtttttt tataacaatg tgttcaccac
    30361 atgattacct aaattgtaag aatgagcgtg ccccaggtca agcctccacc gaagcctgat
    30421 agaagaacag tctggctacc atctaaaggg atgagacctt gttctacaca ctctgaaagt
    30481 aaaatcggga tactggctgc actggtattg ccatattcca tcatattggc tggaagtttg
    30541 gctcggtcaa caccaatttt tctagccatc ttatccaaaa tacggttatt ggcttgatga
    30601 agtagcagat aatccaagtc tgtcacctct ataggagatt catcaatagt ctgcttgata
    30661 gacttggcta catctcgaat ggcaaaatca aagactgtgc gtccatccat cttcaaaaat
    30721 gaatctgcac tttcttgatc tgaaaatgga gaatgcaaac ctgaatgccc ataagttaaa
    30781 cactcgctgc gacttccatc gctattgaga ctctcagcta agaaatgctc ttgctcgcta
    30841 gcttctaaca agacaccacc agcaccatct ccaaacaaca cagctgttga tcgatccgac
    30901 caatcgactg ccttagagag ggtttcacta ccaatcacca agcctttttg aaagcgacca
    30961 gaagcgataa acttttcagc agttgaaaga gcaaatacaa atccactgca agccgcggtt
    31021 aagtcaaaag caaaggcttt attagcacca atattagctt gaacacgagc agctgtagag
    31081 ggcatcatcg aatctggagt aatggtagct aggatgataa aatccagttc ttctcctgtt
    31141 attccagctt ttgccatcag tttcttagca acctctgtag ccaaatcact ggtagattct
    31201 gttcttgaaa tatgcctttg tcgtattccc gttcgacttg aaatccactc atcattggta
    31261 tccataatct gagccaagtc gtgatttgta accacttgct ctggcacata atgagcaacc
    31321 tgacttattt ttgcaaaagc cattatttca aatcctccaa aaattggtaa agattagtca
    31381 agcctttacc catgacagca atttcttcct cgctcatgcc atcaataatt ttttctacca
    31441 tggccttgtg gaagcgttta tgcagtctat gaatcaagcg acccttcttt gtcaaatgca
    31501 gatgcaccac acgacgatcc tgttctgacc gaactcgctc aatgtagccc ttacgctcta
    31561 aattgttcaa acttgtcgta acagttccaa gagttaccat caactctttt gacacttgac
    31621 ttggagtcac atctggagcc ttaccgatga catcaatcgt atgcatttct ttgatggaga
    31681 tatccttaaa acgactacct ctcaagttca cttcctcaat tacaaggaca ttgttaaata
    31741 tagatgttaa atattcattg attcgttggt agtccatttt tcatatccct ccttcttcaa
    31801 aaaactttta caatcaaaac atttgacaga aagaattata tcaaaattca aagaatgatg
    31861 caagtatttt ttattttcct gtaaatttag gtctccttct ctccgaatga gctcgcactc
    31921 cttctttgaa atcctctgtt tgagctaagg atttctgtag gttcagttct aaagtagcgt
    31981 aaccctgcca gtctttaaat tggctctccc aaaccaactt cttaatggca gcataggaat
    32041 tacttgaagc acgtcttaat tttttaagaa gctgttctct cgtcttttca agtttttcag
    32101 cttcagaaac gcggtaaacc aacccccatt ctagggcttt ttctgccgtt aaagcctctc
    32161 ctgtcatagc taattgagca gcacgcgtca caccgatact gcggctcaag agatgaattc
    32221 cccctgcatc tggagccaaa ccaacaccaa caaaggcttg gataaactta gccttatccg
    32281 tcgccaaaca gaaatctgcc gcaacagcca tattcgctgc ggcacctgca acagcaccgt
    32341 caacttccat taaaacaggt ttggctattt gcttgatttt ataagaaatc gtattgacca
    32401 actctgcgat ttttgtcaat gatggaatat catcctcatc cactgcccgc ttcatctcta
    32461 ccaaatctcc cccaactgag aagacctttc cattggcatt gattaagata aaatgcacag
    32521 ctggattttc ttctgccaaa gtcagagctt ctaaaatctc ctcacacatg ggaatatgaa
    32581 aaccatttgc gacctcagga cggttcaagg taaggattgc caagtcctct tcaagctgat
    32641 aaataatgtg ttccatcagg gctccttttt attttataag cagtttatac tcttcgaaaa
    32701 tctcttcaaa ccgcgtcagc ttccatctgt aacctcaaaa cagtattttg agctgacttc
    32761 gtcagtctta tttacaacct caaagcagtg ctttgagcag tctgcggcta gcttcctagt
    32821 ttgctctttg attttcattg agtgttaaat tatttcactg tcaaagtata tcttttttag
    32881 gagcagaaag caagaaaaaa tcgactgaaa gtgtctcagc cgataagaaa ggctcgcttt
    32941 aggctagaaa gcatcttatt ttcttcattg aaaaaagcct ttctttctgc tataatcgta
    33001 taaaatactt actttaggag ttcttatgaa agttgttaaa tttggaggta gttctcttgc
    33061 ctctgctagt caattagaaa aagttttaaa catcgtcaaa agcgattcag agcgtcgttt
    33121 tgtagtcgtt tctgcgcctg gtaaacgcaa tgctgaagat actaaggtta cggatgccct
    33181 gattaaatac taccgcgact atgttgcggg taacgatatt agcaagagcc aaagctggat
    33241 tatcgaccgc tatgctgcta tggttagtga attgggacta aaaccagctg tgctagaaaa
    33301 aatttctaaa agcattcacg ccttggccac tcttcctatt gaagaaaatg aatttctcta
    33361 cgatactttc ctagcagccg gtgaaaataa caatgccaaa ttgattgctg cctactttaa
    33421 ccaaaatggt atcgatgcac gctatatgca ccctagagaa gctgggattg tggtcacaag
    33481 tgaacctggt cacgctcgca tcattccatc aagttatgac aagattgaag aattgacaaa
    33541 caccaatgaa gtccttgtca ttcctggttt ctttggtgtc actaaggaaa atcaaatctg
    33601 tactttctca cgtggaggat ctgatattac aggttctatc attgctgctg gtgtcaaagc
    33661 tgacctctat gaaaacttta cggacgttga tggtatcttt gcagcccacc ctggtattat
    33721 ccaccaacca cactcgattc ctgagttgac ctaccgtgaa atgcgcgagt tggcctatgc
    33781 aggcttctca gtccttcatg acgaggctct tcttcctgcc taccgtggaa aaattcctct
    33841 ggttatcaag aataccaaca accctgacca tccaggtact cgtatcgttc taaaacacag
    33901 taatgatgaa tttccagttg tgggaattgc tggtgactca ggctttgtca gcattaatat
    33961 gtcgaaatac ctcatgaacc gtgagattgg atttggccgc aaggttctgc aaatcctgga
    34021 agaacttaac atcggttggg aacatatgcc aacaggtatc gacgatcttt ctatcattct
    34081 ccgttctcgc caactaactc ccatcaagga agaagaaatc ctgcgtcagt tggttcaaaa
    34141 ggctaaagta gatcatgcag aaatcgaaca cgacctttct atcattatga ttgttggtga
    34201 aaaaatgaag agccatatcg gagtgactgc tactgcgaca cgcgctctat ctgaaaacaa
    34261 aatcaacatc cagatgatgt ctcaaggttc tagtgaagtt tctattatgt ttgttgtcaa
    34321 taaagaccaa gagaaagcag caattaaagc cctctacaat gccttttttg gagaaagtaa
    34381 ggaagactaa gcatggctca atcacttaac aaaacagtac tcctcagtac gacaggcact
    34441 tcctacctct ctatcgctgg gaaagttggg aaattccttg tcggagatca ggctttggaa
    34501 ttttacccag atgtcaatgt cgaacaattt atccaaattc cttggagcca tatcaatcag
    34561 attggagcca atgtcactgg acgcaaaatc agccgccact tcgaagtctt tacagacaaa
    34621 ggaaaattcc tctttgcttc aaaagactca ggtgccattc tcaaaattgc acgcgaaaaa
    34681 ctaggcaatg acaaggtcgt caaacttccg accctgattc agacaattag ccaaaaattt
    34741 aaaaatctat ttgcaaaaaa gtagaaactt tagtataatc atagcaacgg ataagtaggt
    34801 tatcttcttc tttcagaaag tctgcgggta ctgcgagcag ataggaggga taggtgaaat
    34861 tctaccgtta gtaacaatta catacacaat caagtgcaca cctgtgaagt tggatggaac
    34921 cgtggccctg ccactccaac gttttgtcag gcgtgctttt ttcataaagg agttcttatg
    34981 ttagatatca aacgtattcg tacagatttt gaagctgtcg cagaaaaatt agctacacgt
    35041 ggtgtagatg ctgctgtctt gaatgaaatg aaagaaatcg atgctaaacg tcgtaacatc
    35101 ttggtcaagg ttgaaactct caaagcagaa cgtaacacag tttctgctga gattgcccaa
    35161 gctaagcgca acaaggaaaa tacagatgac aagattgctg ccatgcaaaa tctatctgct
    35221 gaggttaaag ccttggatgc tgaattggca gaaatcgatg ctaaattgac agaatttaca
    35281 acgactcttc caaatatccc agctgacagc gttcctgttg gggctgacga agacgacaat
    35341 gtggaagttc gccgttgggg tactccacgc gagtttgact tcgaacctaa agctcactgg
    35401 gatctcggtg aagaccttgg tatccttgac tgggaacgcg gtggtaaggt aacaggcgct
    35461 cgcttcctct tctataaagg cctcggtgct cgtttggaac gtgctatcta caactttatg
    35521 ttggatgaac atggaaaaga aggctatact gaagtcatca caccttacat agtcaaccat
    35581 gattctatgt ttggtactgg tcagtatcca aaatttaagg aagatacttt tgaactcagc
    35641 gataccaact ttgtcttgat tccaactgct gaagttcctc tggcaaacta ctaccgtgat
    35701 gaaatcttag acggcaaaga tcttccaatc tacttcactg ctatgagtcc gtcattccgt
    35761 tctgaggctg gttctgccgg tcgtgatacg cgtggcttga tccgtttgca ccaattccac
    35821 aaggttgaaa tggtcaaatt tgccaaacca gaagaatctt acgaagaatt ggaaaaaatg
    35881 acagccaacg ctgaaaacat tcttcaaaaa ctcaaccttc catatcgtgt cgttgctctc
    35941 tctactggag atatgggctt ctcagctgct aagacttacg acttggaagt gtggattcca
    36001 gcacaaaaca attaccgtga aatctcaagc tgttcaaaca cagaagattt ccaagcccgt
    36061 cgtgcccaaa tccgttaccg tgatgaagca gatggcaagg tgaaactcct tcataccttg
    36121 aacggttctg gacttgcagt tggacgtaca gtggctgcaa ttcttgaaaa ttaccaaaat
    36181 gaagatggtt ctgtgaccat cccagaagca cttcgtccat acatgggtgg agctgaagtc
    36241 atcaaaccat aaaaaataag gtttagctat ttctagctag accttttttc gtaaccaaat
    36301 cagataagca cctagtacaa agaataaaat agttaggcat ataatggttt cagccaatac
    36361 caggtaatcc agaaatggaa gtttcaaaat tccctgagcc atcttgagcg aggtcgctgt
    36421 gataatggtt gggaaggtga gggctgagaa ggctggttga aaaccttgtt ttaaaatgtt
    36481 gggcagacga gttaaaacaa agaaaaagaa ggattgagaa gccaaaatca tgacaatcaa
    36541 gacccaagtc ggcaggctgg ttcctcctac tcgaactaga gaagccaaga gtagagagaa
    36601 aggagcacag tagattcctt cttgtccaag caaggctagt gggagtggat gtttctttaa
    36661 atcgctataa ataaggggat agagatagaa ggtcaagaga aaaccaaaac tcaaggtcgc
    36721 ataggcaatt tcgataatac ctaccagagg ataggtcaag gcagccactg ctatccccac
    36781 atagagaacc gtccaacttg gagtggcatg aaccctccgc cctggacaag caaacttgat
    36841 ggtaaaacca gcaatcaagg tcaaatccaa gagaaatgaa aaccaccaaa tcccttgtgc
    36901 taccaaagga agataagaga atacgcgaaa gacataggtc gataaaatca tcccagccat
    36961 aggaaaggtt gccattcctg acaaaagagg gggcttggtc aattcttgct tggtttcttt
    37021 ccaattaaag agatgcagaa ttagaaagta aatccataaa accaaaccag tcagactaaa
    37081 aagatgggat agaaccggca acgtatctaa aataagattt ccagctcctg ccaaacctag
    37141 caaacaacct gaaaatacta aggggagttt tttcatccta acctccaata atcatgttag
    37201 tttcagtata acataaaagc gcttaaatga ggatttaaaa aaacgaagtc cgcttatttc
    37261 agatttcatt ttactcagat atgaattagg cataaggttg caattctgga ttaattggtg
    37321 tattagctaa gttgttggca tagttacaga ggattgctag gctgacacca aaaaccacat
    37381 ccaaggcatt ttgttgagtg tagccagctt ctaaaaactc agacaaggct tcatctccta
    37441 cacgaccctt ggtattgata actgccaagg taaacttagc tagggtatcc aatttaggat
    37501 ctgtttcaat tggagtacga ttgcgaagag cttgaatcaa gtcatcattc atctggattt
    37561 gtttgatgga aaaggctgtg tgacctgcga cacagaaggc acaaccattg gtcacggctg
    37621 ccgtgatttg caccacttca cgctcaacgg gtgtcaggct gttgcgacgg tggatagatg
    37681 agacaatttg gtaggcttct aaaacagtcg gggcattggc caagagaccg attaggttgg
    37741 gaatatagcc attgttgtct ttttctactg tttcaagaat ttctttcact tctgctggtg
    37801 ctgactctac tgtatggata gtaaatgttg tcataagata cctcttttct tattattgac
    37861 actaatatta ttggaaaatc ttataaaatc ctgattccta agtttatcta agataaagct
    37921 ttattctctc ataagatttt cgttgttata ttagtttatc acacttccaa tcacttgcat
    37981 aatatatatt atatatcagg ctgataaaaa ttatttatag gcaaaaaaat cacacgagct
    38041 gtgtgattcc attatttgtt aaaatacttt ttagtttcag caataacgac tggcgacaag
    38101 accaagaggg caatcaagtt tggcagagcc atcaaggcgt taacgatatc tgcgataatc
    38161 cagaccatat ccaactcgat aaatcctcct aacaagacca tgagcacaaa aaacacacgg
    38221 tagagccaga taaagcgagc cccaaagagg aactcaaaac agcgttctcc gtaatagttc
    38281 caacctagaa tcgttgtaaa ggcaaaaagt acaaggaaga tggtcaagag ggcaggccca
    38341 aagtgtgaaa agactgttga gaaagctgac tgagtcaagg caaccccatt caagtcacca
    38401 ctccaaactc cagttaccaa gatggtcaaa ccagttagag tacaaatgat gagggtatca
    38461 ataaaggttc ctgtcatgga aatcaaacct tgctctactg gttcatttgt cttggcagct
    38521 gcagctgcaa taggagcaga acccagacca gattcgtttg agaacacacc acgcgccaca
    38581 ccattttgaa tagccatccg aacgctagca ccagcaaatc cacctaccgc agcaagggga
    38641 ctaaaagctg aggtaaagac taaagcgatt gtgccaggga tttttccgat attaaagaaa
    38701 ataactgtaa gagttcctaa gatataaatg atggtcataa aaggaacaac agtagttgaa
    38761 accttagaaa tagacttgag tccaccaaag gctgcaatcg ctacaaagac agacaagacg
    38821 agagctgtga tggctgacga aatcgtcgtt gtattttgga tagattctgt aatcgagttg
    38881 acttgggtga aggttccgat tcccaagaga gcaaccaata ctcctgctac tgcaaacaaa
    38941 acagcaagtg gtcgccactt ttctcccatc cctagaagga tatagtgcat gggacctccc
    39001 gctactgcac catggtcgtc cttggtgcgg tatttgatgg ccaagagtcc ttccgcatac
    39061 ttggtagcca ttccaaagaa agccgccatc cacatccaaa atagagctcc tggtccacca
    39121 accttgatag ccgtcgcaac tcctatgata tttcctgttc caacagttga tgccaaggct
    39181 gtacacagag ctgcaaaact ggatacatca ccatgtccct tatcctggat aaaaataagc
    39241 tgaaaggcct tgggcagacg caaaacctgc aagagtccta gccgaatagt taggtaaatc
    39301 cctgttccga ccaataaaat caagaggggc ggtccccaag caaaatcatc gattgattta
    39361 agcaattcta acatttcctt ctcctatcgt ttcaacccca aaagaaagag cacatgcaag
    39421 atacatgtac tctggaatgc ttagataaat gctaaaaagc ggtctatcct agctctgtcc
    39481 ttttacctga gagtttgagc agttgcctgc cttgcccctt cggtgccttt acggtctctc
    39541 cagagttccg tccatttaca gtcatggaaa atcaaacgat tccccacttc tattaaactt
    39601 cattcggtgt tggtatttaa ttgattctaa ttttacaaaa aatgttggct tttgtcaatg
    39661 tgtttattag taaaaattag ttcaacagtt tttactttat aaagtccaga atactgctat
    39721 cctttaaaaa aaagtgacaa tagtcgcacc actgcctcca gcattttgtg gggcatagcc
    39781 gaaactcttg acatgtttgt ttctttgcaa gtatttggta actccttcac ggatgactcc
    39841 tgttccgata ccatggatga tatcaacttg agccatattg ttaagcaagg cttggtcgat
    39901 gaagatatct agctcattca tagcttcttc atagcgcttg cctcgaagat ccagtctagc
    39961 ttgaggtcct cgcccagaag ttcgtttcac aacattgacc tgcttcttct tgactggttt
    40021 ttcttgctgg gcttgaacaa gatcaaactc tttctcttcc aaggtcatct taatcaagcc
    40081 aacttgggct tcccagcggc cgtccttgag ttgactggtc aaggtaccac gctgaccata
    40141 actgagaacc acgatatcat ctcccacctt tggagctcgt tttttcttgg ccttttgaag
    40201 gaccttgttt ttagacaagt ccactttttc aggagccaat tttttcaact tggctttggc
    40261 ttcaatgatt tcgtggggct taagttggga tttactgtgg agatttttga gaatctggtc
    40321 actttcactt agggccatat ccacaatctc agcagcctgt tcacgcgcct tgttaagctc
    40381 ggtttccttt tcacgattaa gctcgttgta gagttttttt agcgcacggt tcatcttgag
    40441 attttcttgc tccacctcac ggatattgtc caaacgtttg cggctttcca gcgtctgctc
    40501 ttctaattgc tcaatgatac gattgacgtc attgtcctga tcgatctgct gactggcatc
    40561 tcctacgata acttcagata ggcctagacg tttggcaatt tcaaaggcat tacttcggcc
    40621 aggaacaccc tgcataaagc gataggtcgg gcgaagagtt gcagtatcaa actccatact
    40681 ggcattttgc acaaaggctg tctcaatacc gtaggccttg agttctggat agtgggtcgt
    40741 cgccatggtc ttgatttgac gcaggcgaag gtcctccaga atagccatgg caagggctgc
    40801 tccctcttgg ggatcagtac cagcccccaa ctcatccaaa agtaagagtg aatgttggtt
    40861 gaccttgcca agaatatcca cgatattggt catatggcta gagaaggtag acaagctctg
    40921 ctcaatagac tgctcatctc caatatcagc aaagatttct tcaaaaatac caacacgact
    40981 tcccttgtct gctaaaatcg gcaatcctga ctgggccatg acctgtgtca agcccagagt
    41041 tttgagcatg atggtcttcc cacctgtatt gggacctgta atgacaatag ctgttaaatc
    41101 ttgaccaaaa tagacatcat ttgcgacggc atttttgacc aaaggatggc agacatggag
    41161 cagttgaatc tcttgatttt ctgacagctg aggcacgact gcttgtcttt cttggataaa
    41221 tcgaaccttg gcacgaatca agtccagatg accgataatc caagcgtcat tagcaatctc
    41281 agccgcatga gggcggacac gctcagaaat ttcttggaga atgcgaagca tttcatagcg
    41341 ctcatctgct cgcagactag caatttcttc gctcagtttg actacctcac gtggttcgat
    41401 atagacggtg tttccactag cagaaatatc atgaacgaca cctgcaatct tattgcggta
    41461 ggtgtttttg actggtaaaa cctgacggcc atttctgcta gcaacaattc cttccgtcaa
    41521 catctgcgct ttttgcttga gtaagtcttg taaaacatcg cgcacctgac tctcgctatc
    41581 atgtattttt cgacggattc gcgccaattc ttcactggca aaattttcaa tgaaacccgc
    41641 atcattaaag gcctgaagat ttccttgtaa ttgcggaaaa tcatgtaatt tctcaaacca
    41701 aagggctaat tcttccaagc tgacattttc cagattggcg taaaaatttt gaagttctcg
    41761 gctggcaaga agcacgcgtt tcaagagtag gaactcctcg atattgagat ccgctcccat
    41821 ctccaacctc ttgcagactc ctgcaatttc cttagttgag agaatagtaa aatgcggttg
    41881 ctcgacgaaa agagcctgca tttccttcat ctcagcaaaa gcctgtttga ttttatctgc
    41941 tttggcagtc ggaaccagtt gtctcaattg ctccaagccc tgctcggtca acaaatgagg
    42001 ctcaaacaag gccttgacct tatcgaactc taatgtttct aatattttct tattcatctt
    42061 tatttccttg tctttgaatg tctaggtgtg gtagcttttt acattctgat agattcgagt
    42121 caaatctgta aacaaagggc taggaaaact cctgcccttt ttatccgatt aaatttgtca
    42181 cccagatttg tttgagccaa ctggttgtta ccggtatgct ctggatgatg tgttttgcga
    42241 cgatactctt ttcaagagga ttttgtataa ctgccatggg gatggtcgcc aagattgtca
    42301 aggccatttg caagacaaat aaggtcacca acatggacaa gatacctgct gaaacttgga
    42361 acaacttacc acccagtttt ttgctaggaa tcaagtgtaa gagaagacca agtaaacgac
    42421 caatactata gacaatccca aatacaagca agtagccgat acctgcataa aagaccttat
    42481 ccagctgaaa gagttgatcc gatgggaaaa agaaagtccc ctgaccttcc tgcgaatttg
    42541 cataagggag caataaatgg aattgctctc caagcccctt ataaaactgg ccagccataa
    42601 aagccgatgc catggctgaa atcaggtaat aaacctgtaa gagcaggcct ctccgatagc
    42661 cgatataaaa tccccaaacc aagaccaata gaagaaggag tgaaatcata aggaatcctc
    42721 aatcttgctc tgttcttgct tacaagtcac aagcttgtga cggagttctt ctagctcttg
    42781 ctccttatcg tcaaattcaa tctcacggct gagctgagtt gataaacagt tgactgccaa
    42841 caaaagagcg attgtttcat catctgcgct aggcatttgt tctttaattg cttggtattt
    42901 ttctgtcgca accttagcga tttcctccat aaaaaggtta tcatgttcac ttgtcaaggt
    42961 taacgatttt ttcccgaatg taaatttgaa tcgatttaga tttgccataa aattcacctc
    43021 acgatattat accaaaattc gctaattttg tcagttttta caaattttac tgcttttgtg
    43081 gtacaataga aactatggca agtatcacac tcacaccaag cgaaaaggat attcaggctt
    43141 ttcttgaaca ctatcaaacc agtctggctc ctagcaagaa tccctatatc cgctactttt
    43201 tgaaactacc tcaagcaacg gtttctatct atacttctgg aaaaatcttg cttcagggtg
    43261 aaggggctga aaaatacgcc agtttctttg gctatcaagc tgtagagcaa accagcggac
    43321 aaaatcttcc tttaattggg acagatgagg tgggaaatgg ttcctacttt ggtggacttg
    43381 cagttgtggc tgcctttgtc acacctgacc agcacgactt tttacgaaaa ctcggtgtgg
    43441 gggattctaa gactctgacc gaccaaaaga tccgtcagat tgctcctatt ctcaaggaaa
    43501 aaatccagca ccaggcactc cttctctcac ccagcaagta caacgaggtc atcggagacc
    43561 gctacaatgc tgtttcggtt aaggttaccc tccataatca ggctatctat ctcctccttc
    43621 aaaaaggtgt tcagcctgag aaaattgtga ttgatgcctt taccagtgct aaaaattatg
    43681 acaagtactt ggcacaagag accaatcgtt tcagcaatcc tatcagctta gaagaaaagg
    43741 ctgagggcaa atacttggct gtcgcagttt cttctgtcat tgcgcgtgat ctctttctgg
    43801 aaaatcttga aaatttggga cgagaactgg gttatcagct tccaagtgga gctggaacgg
    43861 cttctgacaa ggtggctagc cagattttgc aagcctatgg tatgcaggga ctcagcttct
    43921 gcgccaaatt gcactttaaa aacactgaaa aagcgaaaaa acgcttagaa aggtaagtta
    43981 tgaattcatt taaaaatttc ttaaaagagt ggggattgtt cctcctaatt ctgtcattac
    44041 tagctttaag tcgtatcttt ttttggagca atgttcgcgt agaaggacat tccatggatc
    44101 cgaccctagc ggatggcgaa attctcttcg ttgtaaaaca ccttcctatt gaccgttttg
    44161 atatcgtggt ggcccatgag gaagatggga ataaggacat cgtcaagcgc gtgattggaa
    44221 tgcctggcga caccattcgt tacgaaaatg ataaactcta catcaatgac aaagaaacgg
    44281 acgagcctta tctagcagac tatatcaaac gcttcaagga tgacaaactc caaagcactt
    44341 actcaggcaa gggctttgaa ggaaataaag gaactttctt tagaagtatc gctcaaaaag
    44401 ctcaagcctt cacagttgat gtcaactaca acaccaactt tagctttact gttccagaag
    44461 gagaatacct tctcctcgga gatgaccgct tggtttcgag cgacagccgc cacgtaggta
    44521 ccttcaaagc aaaagatatc acaggagaag ctaaattccg cttctggcca atcacccgta
    44581 tcggaacatt ttaagagacc taagaggccg agaatcacca atctcagcct cttcttctat
    44641 cgtgagaagt gattgtttat tgacaaatac tcaatgaaaa tcaaagaaca aactaggaag
    44701 ctagccgtag gctgcttaaa acagtgtttt gaggttgtgg atagaactga cgaagtcagc
    44761 tcaaaatact gttttgaggt tgcagatgga agctgacgtg gtttgaagat attttcgaag
    44821 agtataaaat aaaaacaatc ccaactctca cctattcatg caaaggaatt ttatggaagt
    44881 ttatttttca ggaaccattg aacggattat ttttgaaaat cccagcaatt tttatcgcat
    44941 cctcctccta gaaatcgacg atacggacgc agaggatttt gatgactttg aaatcattgt
    45001 cacaggaacc atggctgatg taattgaggg cgaagactat actttttggg ggcaaattgt
    45061 ccagcactcc aagtatggag aacaactgca aatcagtcgt tatgatcgcg caaaaccaac
    45121 tagtaagggc ttggtcaagt acttttcaag tagccatttc aaggggattg gtctcaagac
    45181 agctcagaaa atcgtggata cctatggcga aaataccatt gacgaaattt tgcaacaccc
    45241 agaaaagtta gaaggcatcg caggactctc tgccaaaaat cgcgaggctt tcgtctccac
    45301 tctccgtctc aactacggaa cggagatggt tttggccaaa ctagccaact acggcattcc
    45361 caacaaacta gcctttcaga ttcaagactt ttacaaggaa gaaacccttg atgtggttga
    45421 aaattatccc taccagctgg ttgaggatat caagggtttg ggctttacca ttgctgacca
    45481 actagctgag gaactaggca tcgaaagtca ggctcctgaa cgcttccgcg ccggtctagt
    45541 tcacagtctt tttcaggcct gtatggaaac aggggacacc tatgttgaag cacgggattt
    45601 gctggaacaa acccttactc tccttgagtc ttcccgtccc gtagaactag accccagcca
    45661 agttgcccaa gagctctcct acctgatcga agaagacaag gttcagcaga ttgataccaa
    45721 gatctttgac aacagcctct ttttcgctga ggaaggtatc cgcagtcact tgattcgtat
    45781 ccttgaaaaa ggaaaacaga agagccaaga tttagaaact attcaaaaac atatcactac
    45841 tgtcgagcaa gaactgggga ttgagtatga taacattcaa aaacaagcta tttgtgacgc
    45901 tatccagaac aaggtcttta tcctgacagg tgggcctggt actggtaaga caactgttat
    45961 caatggaatc atcgctgttt atgccctttt agaaggactt gacttcagga agaaaagcaa
    46021 tctgcccatt cttcttgctg ctccaactgg tcgagctgct cgtcgcatga atgaattgac
    46081 aggtttgcct agcgcgacca tacaccgcca cttgggaatg acaggtgacg atgataccag
    46141 tcatctggaa gattacctag atgctgactt tatcatcgtg gatgaattct ctatggtgga
    46201 tacttggctg gccaatcaac tcttctccaa tatctcttct aacagtaaga tcctcatcgt
    46261 aggtgacagt gatcagctac cttctgtcag tcctggacag gttctagcgg atctgcttca
    46321 tattcctttg attcctcaga ctcgcttgga aaaaatttat cgacaaagcg aagaatcaac
    46381 catcgtcacc ctagctagtc agattcgaca gggcatcttg ccagctgatt tcacccaaaa
    46441 aaaagctgac cgttcctact ttgaaattgc tagtggccat attcctgcca ctattgaaaa
    46501 aatcttaggt gccgccctca gaaatagtat tcctgcccgt gatatccaag ttctggctcc
    46561 tatgtaccga gggacggcag ggattgatgc tatcaatcag ctcatgcaag acctgctcaa
    46621 tcccccacaa aaagatcaac tcagttttga agctcctcag tgccactatc gtaagggaga
    46681 caaggtcatt catttggtta acgatgctga aatcaatgtc tttaatgggg atttaggagc
    46741 tatcacagac ctgattcctg gtaaatacac cgaatcgaaa caagacgaga ttgtcattga
    46801 ttttgatggt aatgaagtct cttacccccg taacgaatgg tacaagattc gcttggccta
    46861 tgccatgagt attcataagt ctcagggaag tgagttccca gttgtcatcc taccgattac
    46921 tagtgctagc cgacgtatgc tggagcgaaa tctcatctac acagccatta cacgtgccaa
    46981 aagcaaactc atcttactag gcgaattaca agccttcgac tatgctaccc aacacatcgg
    47041 aactgcccga aaaacctatc tgattgaacg cttcagtgat ctactggaga atgttgaaga
    47101 aaagcaacaa gctgtctctg aaacagtcac atcaagtgcc tctgaacaat cctacatcct
    47161 aaccgaagaa aactgggaca gcattccagc catgattggg attacagaca cagacctcaa
    47221 agagattttt ggaaaatagt acatcagaaa acccaccaat tcaggtgggt tttctgttta
    47281 ttaagattat tttactgttg ctgtgcttgt gatcaattca acagcttttt tgatagtgat
    47341 atcatgtttc aacatgtcag ctgaaagcaa gttttgaact tgtgcaactt ccatgttgta
    47401 gtctgctgcc aattgctcaa cttctttttg tatttcttct tctgaagcat caaatccttc
    47461 agctttggca actgcttcga taacaaggtt agtcttagta cgtgactcag cttctgcttg
    47521 gtattggttg tgaaggtctt cttgagtagt tccagtgatt tggaagtaca tgtcagggtt
    47581 gatcccttga cgttgcaagt tcccaaggaa ttcatttact gaacggtgaa cttcttcatg
    47641 gatcatttct tctggaagtt ctacgatttc agcattttct acagctgtat caattgctgc
    47701 accttcaact gcatctttgt aagcttcttc tttagcagca gccaattctt tgcggtattt
    47761 ttctttcaag tcagcaagtg tttcaacttc ttcatcaatg tcttttgcaa gttcatcgtc
    47821 aagagctgga acttctttag cttttacttc gtggatagtt gtcacgaatt tagcttcttt
    47881 acctgcaagg tcttctgctt ggtagtcttc tgggaatgtt acgataacat caacggtttc
    47941 gccagctgag tgacctacca attggttttc gaaaccaggg atgaattgac ctgaaccaag
    48001 tccaagtgag aagttttcac cttttccacc gtcaaattca acaccgtcga tagaaccaac
    48061 gaagtcgatc acaacagtgt cgccgttttc agcagcagct tccttgataa ccaattcagc
    48121 cagattgttg cgttcgcgtt cgatacgctc ttcaacatca gcgtcagtta cttctttttc
    48181 tacatcaact gatacttcaa ggtttttgta gtcacccaat tttacttcag gttttgtaac
    48241 gacttcagca gcgataaccc agtcttgacc tttttccatt gaagttacgt caatttttgg
    48301 ttgggcaacc acttcaagac cagcttcttt tacagctgct tcataagcgt ttggcaaaag
    48361 agcgttcata acgtcttggt aaagtgattc ttcaccaaat tttttgtcga agataggacg
    48421 tggaaggtga cctttacgga aacctggaac attaagagat ttcttcactg acttgaagac
    48481 acggtccaat tctggtttga tttggtcttg agagatagtg aaagtcaaga cgccacggtt
    48541 tgtttctttg ttttcaaatg atacagacat tctgtcattt ctccttaaaa ttttttaaat
    48601 acagtctatt ataacatttt ctataaataa aatctagact aatgcagcca attatagaca
    48661 acaagccttt ttcctaac
//
LOCUS       DAVBGY010000008        48349 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVBGY010000008 DAVBGY010000000
VERSION     DAVBGY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48349
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.008"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(865..2091)
                     /locus_tag="VQ620_000478"
     CDS             complement(865..2091)
                     /locus_tag="VQ620_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HET2763160.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVFEVGPGDEVIVPAITYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(2134..2826)
                     /locus_tag="VQ620_000479"
     CDS             complement(2134..2826)
                     /locus_tag="VQ620_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET2763161.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGNLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSTEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFKVLK"
     gene            complement(3012..4763)
                     /locus_tag="VQ620_000480"
     CDS             complement(3012..4763)
                     /locus_tag="VQ620_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2763162.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALVDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(4753..6498)
                     /locus_tag="VQ620_000481"
     CDS             complement(4753..6498)
                     /locus_tag="VQ620_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2763163.1"
                     /translation="MSIIQKLWWFFKLEKHRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSPAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTELRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSEVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILNPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGRHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(6704..7000)
                     /locus_tag="VQ620_000482"
     CDS             complement(6704..7000)
                     /locus_tag="VQ620_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HET2763164.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(7153..7609)
                     /locus_tag="VQ620_000483"
                     /pseudo
     CDS             complement(7153..7609)
                     /locus_tag="VQ620_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            7743..8570
                     /gene="xth"
                     /locus_tag="VQ620_000484"
     CDS             7743..8570
                     /gene="xth"
                     /locus_tag="VQ620_000484"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET2763165.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            8582..8725
                     /locus_tag="VQ620_000485"
     CDS             8582..8725
                     /locus_tag="VQ620_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET2763166.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEYQRSEENFLFLLK
                     SVN"
     gene            8725..8964
                     /locus_tag="VQ620_000486"
     CDS             8725..8964
                     /locus_tag="VQ620_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET2763167.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      9348..9443
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            9843..10424
                     /locus_tag="VQ620_000487"
     CDS             9843..10424
                     /locus_tag="VQ620_000487"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HET2763168.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            10424..11686
                     /locus_tag="VQ620_000488"
     CDS             10424..11686
                     /locus_tag="VQ620_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HET2763169.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(11773..12639)
                     /locus_tag="VQ620_000489"
     CDS             complement(11773..12639)
                     /locus_tag="VQ620_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HET2763170.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEEIKAIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNI
                     NDLEHNILKNLK"
     gene            complement(12626..13396)
                     /locus_tag="VQ620_000490"
     CDS             complement(12626..13396)
                     /locus_tag="VQ620_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET2763171.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            complement(13422..14276)
                     /locus_tag="VQ620_000491"
     CDS             complement(13422..14276)
                     /locus_tag="VQ620_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HET2763172.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELMPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYLNNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            complement(14433..14837)
                     /locus_tag="VQ620_000492"
     CDS             complement(14433..14837)
                     /locus_tag="VQ620_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET2763173.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            complement(14907..16388)
                     /locus_tag="VQ620_000493"
     CDS             complement(14907..16388)
                     /locus_tag="VQ620_000493"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HET2763174.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEHVGILLD"
     gene            complement(16407..17585)
                     /locus_tag="VQ620_000494"
     CDS             complement(16407..17585)
                     /locus_tag="VQ620_000494"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET2763175.1"
                     /translation="MAQHLTTEALRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVFENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            17750..18757
                     /gene="galR"
                     /locus_tag="VQ620_000495"
     CDS             17750..18757
                     /gene="galR"
                     /locus_tag="VQ620_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HET2763176.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFRNYSQAKGIYHDELVFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHDRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(18803..19840)
                     /locus_tag="VQ620_000496"
     CDS             complement(18803..19840)
                     /locus_tag="VQ620_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET2763177.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEVDDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATVVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(19841..20194)
                     /locus_tag="VQ620_000497"
     CDS             complement(19841..20194)
                     /locus_tag="VQ620_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET2763178.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(20438..21328)
                     /locus_tag="VQ620_000498"
     CDS             complement(20438..21328)
                     /locus_tag="VQ620_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET2763179.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            21461..21997
                     /locus_tag="VQ620_000499"
     CDS             21461..21997
                     /locus_tag="VQ620_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET2763180.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFQKNQDHITSLLFS
                     KNDYFLRQLQRELEHHVYPMLAADLKQTHPSLPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(22164..22970)
                     /locus_tag="VQ620_000500"
     CDS             complement(22164..22970)
                     /locus_tag="VQ620_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HET2763181.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQARFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(23296..24816)
                     /locus_tag="VQ620_000501"
     CDS             complement(23296..24816)
                     /locus_tag="VQ620_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HET2763182.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLVQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDYLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNKLAGKITESQMSQLNYQVGVEGKSAEQVAKEFLQEQGLLKK"
     gene            complement(24809..25537)
                     /locus_tag="VQ620_000502"
     CDS             complement(24809..25537)
                     /locus_tag="VQ620_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2763183.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            complement(25552..26316)
                     /locus_tag="VQ620_000503"
     CDS             complement(25552..26316)
                     /locus_tag="VQ620_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET2763184.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     CIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(26320..26763)
                     /locus_tag="VQ620_000504"
     CDS             complement(26320..26763)
                     /locus_tag="VQ620_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2763185.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(27445..27741)
                     /locus_tag="VQ620_000505"
     CDS             complement(27445..27741)
                     /locus_tag="VQ620_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET2763186.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETAKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(27763..28827)
                     /gene="pepA"
                     /locus_tag="VQ620_000506"
     CDS             complement(27763..28827)
                     /gene="pepA"
                     /locus_tag="VQ620_000506"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HET2763187.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            29228..29929
                     /gene="galE"
                     /locus_tag="VQ620_000507"
     CDS             29228..29929
                     /gene="galE"
                     /locus_tag="VQ620_000507"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2763188.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            30352..31311
                     /locus_tag="VQ620_000508"
     CDS             30352..31311
                     /locus_tag="VQ620_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2763189.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKEISESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            31301..32257
                     /locus_tag="VQ620_000509"
     CDS             31301..32257
                     /locus_tag="VQ620_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HET2763190.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLTLQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            32254..33006
                     /locus_tag="VQ620_000510"
     CDS             32254..33006
                     /locus_tag="VQ620_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2763191.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGS
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            33102..34067
                     /locus_tag="VQ620_000511"
     CDS             33102..34067
                     /locus_tag="VQ620_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2763192.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPAILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(34292..34534)
                     /gene="yidD"
                     /locus_tag="VQ620_000512"
     CDS             complement(34292..34534)
                     /gene="yidD"
                     /locus_tag="VQ620_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET2763193.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(34534..35256)
                     /locus_tag="VQ620_000513"
     CDS             complement(34534..35256)
                     /locus_tag="VQ620_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2763194.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(35243..35812)
                     /locus_tag="VQ620_000514"
     CDS             complement(35243..35812)
                     /locus_tag="VQ620_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HET2763195.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKSQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(35831..36559)
                     /locus_tag="VQ620_000515"
     CDS             complement(35831..36559)
                     /locus_tag="VQ620_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET2763196.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(36559..37299)
                     /gene="xerD"
                     /locus_tag="VQ620_000516"
     CDS             complement(36559..37299)
                     /gene="xerD"
                     /locus_tag="VQ620_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET2763197.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(37290..37751)
                     /locus_tag="VQ620_000517"
     CDS             complement(37290..37751)
                     /locus_tag="VQ620_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2763198.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(37748..38269)
                     /locus_tag="VQ620_000518"
     CDS             complement(37748..38269)
                     /locus_tag="VQ620_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET2763199.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(38245..39216)
                     /locus_tag="VQ620_000519"
     CDS             complement(38245..39216)
                     /locus_tag="VQ620_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HET2763200.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGKSSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(39213..40007)
                     /gene="racE"
                     /locus_tag="VQ620_000520"
     CDS             complement(39213..40007)
                     /gene="racE"
                     /locus_tag="VQ620_000520"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET2763201.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIYVEHVEL"
     gene            complement(40214..40462)
                     /locus_tag="VQ620_000521"
     CDS             complement(40214..40462)
                     /locus_tag="VQ620_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HET2763202.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(40604..42229)
                     /gene="treC"
                     /locus_tag="VQ620_000522"
     CDS             complement(40604..42229)
                     /gene="treC"
                     /locus_tag="VQ620_000522"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HET2763203.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTGNGIGDFRGIIGKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCIGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(42407..44374)
                     /gene="treP"
                     /locus_tag="VQ620_000523"
     CDS             complement(42407..44374)
                     /gene="treP"
                     /locus_tag="VQ620_000523"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HET2763204.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGNDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            44559..45269
                     /gene="treR"
                     /locus_tag="VQ620_000524"
     CDS             44559..45269
                     /gene="treR"
                     /locus_tag="VQ620_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HET2763205.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            complement(45302..46105)
                     /locus_tag="VQ620_000525"
     CDS             complement(45302..46105)
                     /locus_tag="VQ620_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2763206.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKHLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKLKLLLLDEHTAALDPKTSQMVMDLTQKIVEYHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(46118..46546)
                     /locus_tag="VQ620_000526"
     CDS             complement(46118..46546)
                     /locus_tag="VQ620_000526"
                     /inference="COORDINATES: protein motif:HMM:NF014684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763207.1"
                     /translation="MKTEIGLVLRSTGDNIPMSEANGVNVNTMKIVGYMISTGLIALC
                     GSLFAQNDGFSDVTSGTGTIVVGLSSEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYE
                     IPNLDQNLVRLFNAILLALVLFAPALQKRLKIRGLKLRNE"
     gene            complement(46555..46995)
                     /locus_tag="VQ620_000527"
     CDS             complement(46555..46995)
                     /locus_tag="VQ620_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2763208.1"
                     /translation="MNFVLSSLSEGLLWSIMAIGVYLTFRILDIADMTAEGAFPMGAA
                     IVVSQIQAGANPWLATLLALLAGMVAGLISGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGAVFSLSLVCLLLVWF"
     gene            complement(47040..47945)
                     /locus_tag="VQ620_000528"
                     /pseudo
     CDS             complement(47040..47945)
                     /locus_tag="VQ620_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT        14184 a        11119 c         9050 g        13996 t
ORIGIN      
        1 gtcctattct tatttcattt tattatatct acaacctcaa agcagggttt tgagctatct
       61 atggccaact tcctagtttg ctctttagcc ttccattcaa aagtgatgct atctttctta
      121 gcttgaatat aaaatatccc atttactata tttaaatttg ttgatatgtt gtaaattata
      181 aatgaaagaa caaatatctt gtcatacaag cattcgccag ggatatttac atgaatatcg
      241 ctcatatggc ggattgaaac taaaacacta caatatattt cttaaataga ccttctataa
      301 aagctagatt taacctcgat aaaaatagat gcagtgcttc taaaatactg gaatagggca
      361 tataggtaat cgtagacaag tgttttatag attgtctcgc gggagatcta atgatgtata
      421 gcaagcatca ctaaacatat agaatatttg tgttattata atgtcaatat gctataaatt
      481 ctatcatgca aaagaatgac tagaacatga aagacaggca caaaactttg aacaatattc
      541 tgaaaacaaa aaaagacgcg agtttccaca atctcactca tggaaactca cgtctttcaa
      601 aaaatagggt gaattaaaaa ctagaacaag acactagatt ttctaaatga tgtctagtaa
      661 ggattctaaa tgctacacca aattgctcac atcttatttc aaaccacggg actcccacaa
      721 gaaaggaaaa tcctataaaa attctctctc tgaggagaga gaggcctcaa ctacaggtgt
      781 aaactattat caattgaaaa caacagtttt tacactcaat tagacttttt agggacagga
      841 tcactttcgc ttgactgtag tttgtcattt ttttgataaa gttagcactt tttcagaaac
      901 tgttttgaaa gtctcaatga tatagtctac ttcttcatcg cttaatttag tatgaagagg
      961 gagggtaatt tcattctcaa agaaggcata ggccttagga tagttcgtca tatcaaatcc
     1021 aagattctta taggctgtca agagaggaag cggtttgtag tgaacattac ttgcaattcc
     1081 tgctttagcc aattcttgga tgatgaggtt gcgttcttct aggcttgctc cttctacacg
     1141 ggtgatgtag aggtggcgtg aagattcgac agtttcagtc ttgtgtgcca aaggatggat
     1201 gcgagaacct gcaaaaccac tatcatagcg gtccacaatg tccttacggc gttgcaacaa
     1261 acttggatag cggtccaatt gtaccaaacc aagtgaagcc atgatatcgg tcatgttgca
     1321 cttataggct ggtgtaacga tatcgtattc ccatgacccc agttgcatct tggcaagagc
     1381 atccttagtt tgcccgtgaa gggaaaggat ttggaattcc ttgtacatct cttcgtcatc
     1441 aatcactgga ttggctttcc aagtcgcact tccaccttct gccgttgtaa agttcttaac
     1501 ggcatggaat gagaaggaag taaagtcagc gatagaacca gaaggttgtc ctttataagt
     1561 agatcccaaa gcgtgggcac tatcagagac aatgacaata cggttaaagg ccttttgcca
     1621 cttgcttgaa gcggtaaaga agtcacgttt tttctccacg acttggaaca aacggtcata
     1681 atcgcaaaca atccctgcga gctctactgg gataatcacc ttagttttct cagtgatagc
     1741 ttgctcaagc aggtcatagt ccatctcaaa cgtatctgct tggatatcca ccatgacagg
     1801 ggttgctccc acgtgcgtaa taacactaca tgaagccgta taggttatgg ctggaacgat
     1861 gacttcatca ccaggtccca cttcaaaaac gcgtaaaatc aactccagag cggctgtcgc
     1921 agagttgaga caaacagtct taggtgtctg tgtgtaaaga gacaagcggc gctccagttc
     1981 ttttgtttta ggacctgttg tgatccaacc agaacgcagg gtatccgcta cttcagcaat
     2041 ttctgcttct gtgatatcag gcggtgaaaa tggaatattg taatttggca ttgatttgct
     2101 ccttttatct gtactcattc tcttatgact actttatttt agtaccttaa acacggtttg
     2161 aaacatgatt ttgatgtccc caaagaaact aaactctcgg agataggcga ggttatagcg
     2221 catcttttca ggaagaacat gctccacata ggcctgatca actgacagac ctttctctgt
     2281 catttgactg atgatggtgt cctcatcctt gtagttgatg ctggctggag aggtaattcc
     2341 tgcttgcaag agcaaggttg ccatcatttc agtgctatac tgctctgtat aacgtggcac
     2401 ttcaggtcgt gtaccgacaa aggacatctc acctttaagg acattgacca actgaggcag
     2461 ttcgtccaaa cggacacgtc ggatgaaatt tccaaccttg gtaatacggc tatcgttagc
     2521 agaagtcacc agatttcctt ttttatccgc atccgtcacc atggtacgga acttccaaat
     2581 cttgaaccga cggttgtact gggtcacacg ctcttgcttg taaatcactg gccccttgct
     2641 atccaacttg atccaaatgc tcaagatgag aaagatggga gaggtcagaa ccagtaagac
     2701 caaggccaaa acccagtcca agcaacgctt gaaaatcagc gaacccttcc ttttagagac
     2761 aagctggtag taagactcaa cctcgcttga tttcatttcc acaggcaagt cttcccattt
     2821 cagcatgctg ttctcctttg tttttattat actatttgtc aacacttttt gtcaacactt
     2881 ttcattatac cacaaaatgg aaaaggcggt gaaaaaaatc tgtaatttga aggatttcct
     2941 tctagtactc aatgaaaatc aaagtacagc tttgagattg cagataaagc tgacatggtt
     3001 taaagagatt ttcaaagagt ataggccatg gcccctgcct gcaaactata catcttgtga
     3061 taggttcctc ccagagccaa gagttcctca tgggttccac tctcgataat gcgtccctta
     3121 tccaagacat agatgcagtt ggcgtcttga atagtagaaa ggcggtgagc tatagcaata
     3181 gttgttcggc cctgtctcat cttcgccaga gaagcttgaa ccaagctttc tgtttcagag
     3241 tcaatattgg ctgtcgcttc atccaaaatc aggattttag gctggctggc gactgttcta
     3301 gcaaaggcaa gaagctggcg ttgcccagta gagaagctcg aaccacgctc ggaaacaggg
     3361 gagtcgtacc cctgaggaag ttcttgaata aaggaatctg catccacaaa ggctgccgca
     3421 gcctgaacct gctcatcact gatttcttgg tacatggcga tattggactt aatagttcca
     3481 tgatagagga agggttcctg caagaccaaa ccgatgtttt ttctcagctc ttcttgactg
     3541 aaatccctga tatccacatc atccaagaga actctccctg actggaattc ataaaagcgc
     3601 atgaggacat tgataataga cgatttccct gaacctgtat gacctacaaa ggcaatggtt
     3661 tcacccttat taacagagaa agaaatgtca tccagaatcg gatgtttacc gtcatatgag
     3721 aaacacacat gttcaaagcg gatattgcct tcttggactt tggcttgccc attttcttga
     3781 agaggttcat aggtcctctc gtctaccagg gcaaagacac gacctgcaga aaccatagac
     3841 gtttgcagtg ttgaaaagtt ttgcgtcacc tcaatcaaag ggtcaaagag gcggttgatg
     3901 tactggataa aagcatacat ggtcccggcc gttatcccga tagaaagacc acgatagcca
     3961 aaataggcca tcaagacagc ataacctaga agtttcaaca aactcatggc aggtctcaaa
     4021 aagagggcat ccaaggctac agaacggttg gcatagacca agtgctcttg gttgatttca
     4081 tcaaattctg cctgcaggcg cttctcttga ttaaaggcct ggattattct gattccctcg
     4141 atgttttcag ccagcttgct attgatatct gacaagagac ttctggtttt ctcaatgatt
     4201 ttcactgatt ttttccgata gagattgacc aaaaggaaaa tcaaggggag aaagagcaag
     4261 actaatgctg tcaaacgaaa atccaatacc aacatggtat aaagggttgt cagaaagatg
     4321 aaaactgctg agataaagct agataaaatc ccagaaaaca tatcactaat cgtctcggta
     4381 tcgttggtca aacgagaaac gatagaacct gctggcgtct tgtcaaagta agacatgccc
     4441 agtttctcca tattggcaaa ggcatcccga cgaatatccc taacaatact gtaagacacg
     4501 cgcgcaaaga gaagattgcc gacatactga actacagttt gtaggatgta gagaccatag
     4561 tagaccagca aaacggtaac ggctagttgg ttaagattgc tgagatactg gtcgataaag
     4621 tgggaagcca cgaggggtat gacactttta atgaccgtcg tcgctaggag aaaactgagt
     4681 gccaaaaagg tcaggagtct ataaggcttg agataagaca tcaagcgctt caatacagcc
     4741 cattgttctt gtttattctg catcttcttc tcctttcatt tctaactgct gagactggta
     4801 ggtttgggca taccagccat ccatcgctag caagtcttcg tgcctgcctc gttcgataat
     4861 ttgaccattt tgtagaacta aaataaaatc tgcatggaca acagcactga ggcgatgggc
     4921 agttataatg gttgtcttgt cctttcgcgt ctccttgaga ttgtcgataa tcgcatactc
     4981 tgtcttggca tctacggcgg ataaggaatc atccaaaatc aagatatcag ggtttaaaat
     5041 catagcccga ctcatagcca accgttgctt ttgaccacca gaaagactga ctcctttttc
     5101 accaatcagc gtatcaaatc cttgaggcat gtctacaata tcttggtaaa ctcgggctag
     5161 cttagtagct tcctcaacct ctgaaagggg caagttagga ttgccaaagc ggatattgtc
     5221 taggattgaa gtcgcaaaga gaaactggtc ctgaggaaca tagcccatga gactgcgaag
     5281 ttctgtcaaa cgatagtccc gaatatcgtg accgtttaga taaatggcac ccttatccac
     5341 atcgtattca cgcaagagga gcttgattaa ggacgttttc ccagagcctg tctgcccaac
     5401 caagcccagt gtttgccctt ttgccaaact aaagtgaata tccgtcagtg tttcctcatt
     5461 ttcaaaagca aagctgtcaa tggcatactc caaacgccca ttttcaatac catccagagg
     5521 aaactcaggg tcttgtacag gagattcctg agacaaaaga ttttcaatcc gctggtaaga
     5581 aaccttccct cgctgagtag tattaaagag gaagccgatg gccataagag gccagaccag
     5641 catatccaaa tagctgataa aggtgactag attaccaact gtaatctgcc cttcctgaac
     5701 catcaaggag ccaaccaaaa gcgttaaaac ataggacgaa ccaacaaaca agagaaccat
     5761 agggtcaaag agactatcat atttcatggt ttgcaggttc ttttggaagg ttaattcatt
     5821 gactgcctga aaagacttca actcgtctgc ctgataaccg aaagacttgg tcactttgat
     5881 acctgatacg gactcctgta ccttgttatt gagttcagaa aaagcagctt gggattcgcc
     5941 aaaggcctta tgagtctttc tccctaggcg actagtcgca taggccatga aaggtagggg
     6001 gagaatggca acaagagtca tctgccatga gatgctaaag agcatggtca acaaagtcac
     6061 cagagccgtg atagaggcat ccaccgcaga catgacaccg ccacctgcta aacgagtcaa
     6121 ggcattgata tcattggttg cgtgtgccat cagatcaccc gtccgatagg tttgataaaa
     6181 ggctggcgac atttttgtga aatgcttaaa caagcgagac cgcatgatct gtcccaagca
     6241 ataagaggtc ccaaggatat acatacgcca cacatagcgc aaatagtaca taccaaaggc
     6301 tgcaagtagc aagtaaaata ggctaagaag gaggtcctgc tgggttaatt gccccgatgt
     6361 gatggcatca atgactcgcc ccataaccat aggaggaatg agattgagaa cggaaaccaa
     6421 gaccagggcc acaatcccga ctagataacg gtgtttttct aacttgaaaa accaccagag
     6481 tttttgaata atggacataa aatccctttc tgattgcaaa tggaaacctg aggccaatac
     6541 tcaatgaaaa tcaaagagca aactaggaaa ctagccgcag gctgctcaaa gcactgcttt
     6601 gaggttgtag atagaactga cgaagtcagt aacctacata cggcaaggcg acgttgacgc
     6661 ggtttgaaga gattttcgaa gagtacaaga ccccaggttt ttcttattca taagttacga
     6721 ctgtaacagc acccttgtca tattcagcaa taaagatatt ggctacattg tcatgccctt
     6781 gtttgctgag gttatcaagc aaccactctt cgctacgacc aatcgattcc aagacgtcta
     6841 cttgaatcac accgtcagtc acaactggat acttaggatt ttcatctccc atttgcacaa
     6901 cgatgagttg cccattttgc tcttgcacag ctcgtttgac ttgcttcatc tggaaaatcc
     6961 cttggctacg aagtttgagg gctacatccg atgcagacaa accaactgaa cgacaggctt
     7021 atgggtcaat attcccattt ttgataagga gagttggctt accatcaatc aaacgtttca
     7081 caaagtgaac attgttgttg agctatttga gagtcaagat cagtttgatt aaaatttcga
     7141 tataattaag tgtcatttgt tcacatccac cagttcaatt gcgtctgtct tatacaattc
     7201 gactttctct aacaggtact tgtctggctc acttccgttt agggcacgat aatagcgatc
     7261 tcccaccttg ataagtgcgc catctgtgtt tgtggaagta ttaacatgga cttctgactt
     7321 gtctactccc aaatctttgg aaacaacctc gatgaaatga agtgaagttt gaaatttatt
     7381 gttagaaact tgattgtttt gataagtcgt gatgctgacc aaaagcatag ctaaataagg
     7441 ccagagctga aatcatgacc aactcacgaa atttggttcc ttttttatca tggcatgcct
     7501 tgaaagcaaa aaaaactgtc acagctaata gaacaatccc aaagccaacc atgataccat
     7561 tttgctgacc gatttggctg agtacatagt cataagagta gaatttcatg tattttcact
     7621 ccctttcata aaatcattct taattataaa cgaaagagag taatttttca aaccatacgt
     7681 tggggaaata gatctttttt tggtataata aaatctataa tctgaatgaa aaaggtaact
     7741 ttatgaaact tatctcatgg aatattgatt ccctaaacgc tgccctaact agtgactcag
     7801 ctcgtgccaa attgtcccaa gaagtcctac aaaccttggt cgctgaaaat gctgatatca
     7861 ttgctatcca agaaaccaag ctttctgcca aaggacctac aaagaaacac gtggaaattt
     7921 tagaagaact cttcccaggc tacgaaaaca cgtggcgctc ttcccaagag cctgcccgta
     7981 aaggctatgc tggaaccatg ttcctttata agaaagaact tacacccact atcagcttcc
     8041 cagaaatcgg tgccccttct accatggact tggaaggccg tatcatcact ctagaatttg
     8101 atgcattttt cgtaacccaa gtttacactc caaacgctgg tgacggtctc aaacgcttgg
     8161 aagaacgcca agtctgggat gtcaaatatg cggagtattt ggctgaacta gacaaagaaa
     8221 aaccagtcct tgcgaccggt gactacaacg tagcccacaa tgaaatcgac cttgcaaatc
     8281 ctgctagcaa ccgccgttca cctggattta ctgacgagga acgtgctgga tttaccaacc
     8341 tattggcaac tggatttact gataccttcc gccatgttca tggcgatgtt cctgaacgct
     8401 acacttggtg ggcacaacgc agcaaaactt ctaaaatcaa caatacaggc tggagaatcg
     8461 actactggct cacaagtaac cgtatcgctg acaaggtcac taaatctgat atgattgact
     8521 caggtgctcg ccaagaccat acaccgattg tcttggaaat tgacttgtaa ggattttcta
     8581 tatggactac aatgcggtca ttcccgagtt tcttgtatcg aatatcgaac agtcccggtc
     8641 cttctactgt ggtttactgg gttttaggat tgaataccaa cgttcagaag aaaactttct
     8701 ctttctcttg aagagtgtca actaatgtta gaggagggaa caaaggatca actagctgaa
     8761 ctgacctacc cctttggtcg tggagtcaat ctctcctttg gcataaagga tgttccaaaa
     8821 ctctatcaaa aagtgatgga ggcaaactat cctatttatc gtcccttgac caaaagaaag
     8881 tttcgtgtca gtgatcccta tatctatcct cataaatttg cagttttgga tccagatggc
     8941 tattttttaa gatttagcga gtagaataat aaaaattggg aaaagtcttt aaaagttaga
     9001 aaaaggcata ttgtcaggtg tctaaaaact tgatagtatg ccttttatga tggaaaaatc
     9061 ttctaatact cttcgaaaat ctcttcaagc cacgtcaacg tcgccttacc gtaggtatat
     9121 aatactgact ctgtcaattc tatccacaac ctcaaaacag tgttttgagg ttgtggatag
     9181 cttcctagtt tgctctttga ttttcattta gtataaatgt tagcttacaa tggagttttt
     9241 gttcaccctc tcttctgttt gttcgttttt taaaccaaaa cactaacaaa atagttgatt
     9301 tctaaagtta tttagtgtaa aataaaaaca ttggctcaag cctatttaaa attgaatatc
     9361 gttttacttg tttatgtcgt gaattggcac gacgtttcta caaggtgccg gaacacctaa
     9421 caataagtaa gtcagcagtg agatatggcc gtttttgcca tgcctatttg ggggacttac
     9481 ttagagatta agtaggtctt ttttgaatat ttttaatatt tcccttaatt agtgcggacg
     9541 ggaggtgaaa ttatcctgtg aataatttca ggaatccagg gaatttcatc cctaattttt
     9601 gagcccctag ctctttcatt tctaggctta ggctaaaata gttccccgaa ctattttact
     9661 ctcaaaaatc ccgtcataga caataacaag attattgtct atgataccac tacaacggga
     9721 aatatttgat aagactcgct tcgctcgtaa tagttttatc aaatatatag cattagcaaa
     9781 agggatcact atagaaacat ttaaacaaca agtaaattaa cttttagact ttaggaggtc
     9841 ttatgaaatt attagaagag cgcatcctca aggatgggca tatcttgggt gataacatcc
     9901 tcaaggtaga ttccttttta acccaccaag ttgactttag cttgatgcga gagattggta
     9961 aggtttttgc ggaaaaattt gctgctgccg gcattaccaa ggtcgtaacc attgaagcgt
    10021 cgggtattgc cccagccgtt tttacagctg aagccttaaa cgttcccatg attttcgcca
    10081 aaaaagctaa gaacatcacc atgaacgaag gcatcttaac tgctcaagtc tactccttta
    10141 ccaagcaggt gaccagcacc gtttctatcg ctggaaaatt cctctcacca gaggacaagg
    10201 ttttgattat cgacgatttc cttgctaatg gccaagctgc taaaggcttg attcaaatca
    10261 tcgaacaggc cggtgccaca gtccaagcta tcggtatcgt gattgagaaa tccttccaag
    10321 atggccgtga tttgcttgaa aaagcaggct accctgtcct atcacttgct cgtttggatc
    10381 gttttgaaaa tggtcaggtc gtatttaagg aggcagatct ctaatgcaaa ctcaagaaaa
    10441 acactcgcaa gcagccgttc ttggcttgca gcacttacta gccatgtact caggatctat
    10501 cctggttccc atcatgattg cgacagccct tggctattca gctgagcagt tgacctacct
    10561 gatttctaca gatatcttca tgtgtggggt ggcaaccttc ctccaactcc aactcaacaa
    10621 atactttggg attggactcc cagtcgttct tggagttgca ttccagtcgg tcgctccctt
    10681 gattatgatt gggcaaagcc atggtagtgg cgctatgttt ggtgccctta tcgcatctgg
    10741 gatttacgtg gttcttgttt caggcatctt ctcaaaagtg gccaatctct tcccctctat
    10801 cgtaacagga tctgttatta ccacgattgg tttaaccttg atccctgtcg ctattggaaa
    10861 tatgggaaat aacgttccag agccaactgg tcaaagtctc ttgctttcag ctattactgt
    10921 tctgattatc ctcttgatca acatctttac caaaggattt atcaagtcta tctctatttt
    10981 gattggtctg gttgttggaa ctgccattgc tgctactatg ggcttggtgg acttctctcc
    11041 tgttgcggta gctccacttg tccatgtccc aactccactc tactttggga tgccaacctt
    11101 tgaaatctca tctattgtca tgatgtgtat catcgcaacg gtgtctatgg ttgagtcaac
    11161 tggtgtttat ctagccttgt ctgatatcac aaaagatcca atcgacagca cgcgccttcg
    11221 caacggttac cgcgcagaag gtttggccgt acttctcgga ggaatcttta acaccttccc
    11281 ttacaccgga ttttcacaaa acgttggttt ggttaaattg tcaggcatca aaaaacgcct
    11341 gccaatctac tacgcagctg gtttcctggt tctccttgga ctgcttccta agtttggtgc
    11401 ccttgcccaa atcattccaa gctccgtcct cggcggtgcc atgctggtga tgtttggttt
    11461 tgtatctatt caagggatgc aaatcctagc ccgagttgac tttgctaaca atgaacacaa
    11521 cttccttatc gcagctgttt caatcgctgc aggtgtcggt ctcaacaaca gtaatctctt
    11581 tgtcagcatg ccgacagcct tccaaatgtt cttctcaaac ggaatcgtcg tagccagcct
    11641 actcgccatt gtcctcaatg ccgtattaaa tcataaaaag aaataagaaa aagaggtgcg
    11701 agcctctttt ttgtttatca caaattgatt ataaatacaa tcaatcccaa ttcccatcta
    11761 attgaattgg acctatttca aattctttaa aatattatgt tccaaatcgt taatattata
    11821 taagaatggt aaaatatcaa atgtttctcg taaattattc ttagccttat accaaccttg
    11881 tccatcagta atccacataa actcaacatt aggaatagct ttaatttctt cagcaatcat
    11941 tttgtaagat ctagccgtct catttaattt tgaaccactt cctgaataaa aattaacctc
    12001 aatcagataa agcacttgtt cattttttat gacaaaatca aaacgtttca cagtattccc
    12061 atcattcgta atagcagata aatcgacaga aaaaatttcc tctatttcat tttgttcaat
    12121 ctctttaaat aaattctctc ctaaaatata cccttcagct tcaagataac tttgaacaat
    12181 attttccatc gcatctccag tccgattttt gcgcccatta gtatccatac caacctcaac
    12241 acctgtaacg tagtcaacta aatttgatac taaatgattt tgcaataaat caaatatacc
    12301 tgatttttcc aaaaacatag tatattcttc aatactatag tttcgtttac taaaatcaaa
    12361 atagaaatct tttatcggat ccttaacaat aatcgtatct ctcaaacgtt tagcaataag
    12421 tagaggcaca actttcaaaa tttctggata attttgatat aagtctaaaa aatcctcttg
    12481 tatattttta ctaccaataa gactattcat aatatttaga gcaactttta tactagatac
    12541 atttttataa accttgggaa aatctgtata ataagtccag tcagctactg tgtcggtcat
    12601 cgttgacaac cactcatcaa aatttctagt ttgtttcatt ttctgcctcc aacctcttcc
    12661 ttgcaatttt taaatattct ttttcagcat cgatacctat aaatcttcta cctaaccgct
    12721 tcgcaacaac acccgtagtg ccactaccaa caaatgggtc tagaatatag tcaccctctt
    12781 tagtagaggc taaaataatg cgttctaaca aatactctgg tttttgagtt ggatgtttcc
    12841 cagcccattt ttcaactttc tttgttaaag aacccgtcca gacatctttc atttgttttc
    12901 catcattcaa ttctttcatt aaatcataat tgtagtaatg acgagctttt ttatcatttt
    12961 ttctggccca taaaatggtt tcagtagaat gggtaaaata acgacaagat aaattggggg
    13021 cagggtttgt tttctgccaa gtaatattat tcagaatttt aaaaccttct tgttctaatg
    13081 ccattccaac tgagtatatg ttgtgcaaac tacctgaaat ccataccgtc ccattaggct
    13141 tcagaacttc ttttgctagg cgaatccatt tacgattaaa ctcatgtttt tcttcgaatg
    13201 aagaaatttt atcccaatct cctttatcaa cagaaacaac ttgtccccca gaattagata
    13261 ttccaccatt acttaaaaaa taaggtggat cagcaaaaat catatccata ctttctggtt
    13321 tcatttttga taagaacttg aacgtatctg aatgaacaag aatcatctta tttttattgt
    13381 agtatggttt tgtcataaga acacctccat acttatattc gttatttttc ataatttgtg
    13441 actataattt cagaaatttt tcctcgactt gaagattttg ctccattagt tcgggtagct
    13501 tcaacataat gtatattaaa atccttatac aactcctcta ctaaagcact agaagaattt
    13561 gataacataa cataagctcc tgtatcactc aatctcttaa aggcatctct taatcttact
    13621 tgatctgcaa aagagaatcc ctcatgagta taagacgtaa atgcactcgt ctcagacaat
    13681 ggaatatatg gagggtcaaa atacacaaaa tctcctgttc gaacatctac aattgccttt
    13741 tcaaaatctc ccactttaat ttctagttga ttgttattta gataaactga aatagcagat
    13801 atcaattcct catcaacaat tttaggattc ttataacgtc catatggaac attaaattgg
    13861 ttcttagaat tcacacgata tagaccatta aagttcactc tcaacatata tagaatacgt
    13921 gcagctcttt gtacttcgga catcatatct attctttcat cacgatctgc agaacgtaaa
    13981 tctaaataat attcttttga attatattcc tgatgaactt tcaaaatttc aatcaattct
    14041 tgaggattgt ccttaatttg ttgatagcaa tttattagtt cagcgttaaa atcattaata
    14101 actgcatctt taggagccaa atcaaaaaat aaagctccac ctccaacaaa aggttcgaaa
    14161 tacctgttat aggttttagg cattaattct ctaataacag gcaataattg tcttttacca
    14221 cctgtccatt tcgtgaacgg ttgtaaagta actttcttta tttcttttat cttcatgctt
    14281 ttaattatac catagaaatt aaaacacaac tcttgtattt tctaccacat tactaaattt
    14341 tctattttag aattaaatca tatcaatata aaaaaatgag taaggcaaat cttataaatt
    14401 ccaaaattca cctcactcta ttaaattact ctttatatcc ttacccgtct ttcctcagac
    14461 ctttggcctg tgacaaacat agtaaaggtt gctttgcaga catttctgcc ctcttgattg
    14521 gtgatatcca catccatcac acaggttgta cgaccttgat ggacacattc tcctttaatg
    14581 gtcaatacat cgtcgagttt tcctgcctta aggtagttga tagaggattg gagtgtcacc
    14641 ccatcaagtc ccagcgagat aaccaccaaa ccactgattt ggtcacatag tgtgaataga
    14701 tagccaccat gggcattgcc atagtagttg agcgacgagt tcactacttt ggtcgtcacc
    14761 acaacgtgac catctctcat tttttcaatt tcgtaatttt caaaggcaga tatagcgtca
    14821 aaatgaaagt ctttcatcgt ttcctcctga tatttcaaat gacactatga tactactttt
    14881 tataggactg tgcaaggaga aagctcctag tctagtaaaa ttccgacatg ttccacaaag
    14941 cgcataaagg ctgtctgccc ttgttctgtc tgcttgtaga ctcctgcatc ctcaagtaca
    15001 cgcgcaaaga tagcacccac agagtccttg acgattgcaa gggctttttc tttatccgtt
    15061 aggtctggat gttgggattt gagttggtct gcccactcct gatgataatc ggcaactgta
    15121 acagcttctc ctacaagata gctagcgact tgctctactt cttctttcag acgtggtggc
    15181 aagattgcca agcccatgac ctcaatcaag ccgatatttt ccttcttgat atgttggaca
    15241 tccttgtggg gatgatagat gccatcagga tgctctgctg aagtctgatt gtctcgcaag
    15301 accaagtcca actcaaactg tccatcacgc ttacgcgcta ttggtgtgat ggtgtgatgc
    15361 ggtgtcccgt ctgtctctgc taaaatctgc acactagaat ctgaatactg acgccattcc
    15421 tggaaaatct tatcagctaa gttaatcaaa tcctctttgg aatccgaagt caagcgtagg
    15481 acagacattg gccacttgat aattccagcc ttgacctgct caaaaccagc aaatcggaag
    15541 gtcttttgca agggagccaa ttccatagga aatacgtgac ggcctccctg ataatgatca
    15601 tgagttagaa tagagccccc cacaatcggc aggtcggcat tagatccagc aaaatatcct
    15661 ggaaactgct ctacgatagc caacagacgt tcaaaactct gacgactaat ggccatggga
    15721 cgatgctggc catctaaaaa gatacaatgc tcattaaagt aagcataggg cgaatactgg
    15781 aaaccccatt cctgaccaac catttcaaaa cggataatcc ggtggttgct gcgagctggg
    15841 tggttgaccc gaccatggta accctcattc tctaaacaaa gttgacactg aggataatta
    15901 ctagcttgca ccaacttggc taccgcaatc tctttcggat ccttttcagg cttagagaga
    15961 ttgatggtaa tttcaagttc tccataatca gatggaacac gataagcgat atttttagca
    16021 atggccttga gtttgatgta gtcatttttc tgactgagtt gataaaaatc ctctatcgct
    16081 tgctcaggag agtaggcata ggcttcccag aagtcacgat tgacctgact cggacaagga
    16141 gtcaccaagt ccatcaattc agtaccaagg atttcacgcg cagtctgact atcctcaatc
    16201 gtctctaatc gaacggcttc ctcaactagc tggtccttga ggtcaatcaa tttatccaga
    16261 ttggtctcaa cttccaaaac accttctccc actcgtgcca aaacacgatt ggtcaggtag
    16321 attcgatcca tttcctcaaa tgaactttca gaaatgacat gtgttacaaa tttatctact
    16381 aaggtcacta tagagcctcc ttttgactag tcaaggacgc gagtgccacc tgcaacttca
    16441 gcgatataga agcttggagc gtatccaact acttcctcgt agtgtttgcc tacagcttcc
    16501 ttaaaggcct caacagtatc tttttgaacc aaggcaatgg cacagccacc aaaaccagcc
    16561 cctgtcatac gagcaccgag aactccttct tgtgcccaag ctgtgtgaac aagagtatcc
    16621 aattccaaac cagttacttc ataatcatgc tccagagaaa cgtgtgacgc attcatcaaa
    16681 cgaccaaatg tttccaaatc acctgcttga agggctgctt gagctttaag ggtacgttgg
    16741 ttttcaaaca cagcatggcg agcacgtttc aaacgatttt catctttaat cagatagcta
    16801 tattggtcaa cggcccactc gtccaattca cccagggtct gaatatccaa ggcaacttgc
    16861 aattcttcca ctgctttttc acactcagca cgacgttcat tgtatttaga gtccgccaat
    16921 tcacggcgtt tgttggtgtt catgataaca acgacattgt ccttcaaatc aagtggcacc
    16981 aagtcgtatt ctaaagtatt ggtatctaag taaatagcac gttggtcagc ccccatacca
    17041 atagcaaatt ggtccatgat accagagttg actccgataa agttattttc tgtttgtttt
    17101 ccgattttaa ccaaatcgag acgctctagt tttaaatcaa agagatgctc tgccacgact
    17161 cctgtcaaga gctccaagga tgctgaagaa gacaaaccag caccatttgg gatattccca
    17221 taaacataaa aatcaaaacc tttgtcaatc acgtgcccag cttcttgcaa gaaatgaagg
    17281 acaccttttg gataattggt ccagttgtgc tctttttcaa acttgaggtc agcgagaggc
    17341 acttcgataa tgcccttgtc ctcaaagtta gctgagtaga aacgcaagac ttggtcgtca
    17401 cgcttacgag ctgcaccgta agttcccaag gaaatagcag caggaaaaac gtgcccaccg
    17461 ttgtagtctg tgtgttcacc aatcaaatta atgcggcctg gtgaaaagaa ggtttggtct
    17521 gcttcttgac caaaaacagc aagaaagtct ttgcgaaggg cttcagtagt aagatgttgt
    17581 gccatatgaa ttctcctttg actgtccgtt aagccacctt aacgtataat cgttttcttt
    17641 ctattgtatc caaggaattt tccaagatca atccttttta ctaaaatttt agtaaaatat
    17701 cagtaaaaag aagaaaaaca tgatatacta acctaatcaa ggaggattta tggctacctt
    17761 aaaagacatt gcacagctag cctctgtctc tatcgcgacc gtatcccgcg tcctcaatcg
    17821 cgaccagagc ctatctgtta cagaagaaac cagacaccgt attttaaccg ttgctgaaga
    17881 gctgggctac accaagcacc tcaagacagg cgagtcccac aaacccaagc aaaagattgc
    17941 cattatccaa tgggtcagcg aacaagggga gctggacgac ctctactact accagattcg
    18001 cctaggaata gaaaaaagag cccaagagtt ggactatgat atcttgcgct attttaatga
    18061 ccatcctttt accttaagcg aggaagtgat tgggattctc tgcattggaa aatttagccg
    18121 agctcagatt tctgcctttg aagaatacca aaaacccctg gtctttctag acagtgatac
    18181 actttctctg gggcatactt gtattattac ggatttttac actgccatga aacaggttgt
    18241 cgattatttc ctcagccaag gaatggaccg tatcgggatt ctaacaggcc ttgaagaaac
    18301 aactgaccaa gaagaaatca ttcaggataa gcggctggaa aatttcagaa actacagtca
    18361 agcaaaagga atctatcatg atgaactggt ctttcaagga aactttactg cccagtctgg
    18421 ctatgacttg atgaaggaag ccattcagaa cttgggagac caactcccac cagcattttt
    18481 cgcagccagc gatagtttag ctatcggtgc ccttcgtgcc ctccaagaag ctggaatcag
    18541 cctaccagac cgtgtcagcc tcatttcctt taacgacacc agcctgacca agcaggttta
    18601 tcctcccctc tccagcatca cagtttatac tgaagaaatg gggcgagcag gtatggatat
    18661 tcttaacaag gaagtcctcc acgatcgcaa aattcctagc ctgaccatgc tgggaaccag
    18721 actgacatta agagaaagta ccctaaatca agaataggat aacataaaaa acgaatagag
    18781 ttctaaaact cctattcgtt ttttattcga ttacaatcat agacttaatg gtcttacgtt
    18841 catccatatc tttgtaggct tggtcgatat cttccagttt ataacttgaa gtaaagacgc
    18901 gacctggatt gatatcacca tcaaggacgg cttttagtaa aaattgctta tcgtatgttg
    18961 tagcagaagc tgccccacct gctacagaga tattttgcat aaatgtcgaa ccaagagcac
    19021 gattattata gtgtgggact cctacaaagc ccatacgccc tccattatga agaacaccta
    19081 gcgcctgttc tatagcagcc tccgtaccaa cacattcaag tgctgcgtct gctcctccac
    19141 cgaggatttc acgcaccttg gtaattcctt cttgaccacg ttctgcaaca acaactgtcg
    19201 cacctgactc catagccatc ttttgacggt cttcatgacg gctcataagg ataatttgtg
    19261 atgctccacg catcttagcc gcgatgacag cacattgacc aacagcccca tcaccgataa
    19321 caacaacctt gtcccctttt tgaacatttg caacacgcgc cgcatgatag cctgtcggca
    19381 tgacatctgc aagagtcaaa agggacttga gcatcccttc tgtatagtca gaaggttgac
    19441 cagggatttt aaccagcgcc cagtttgcat agtggaagcg aatatattct gcctgaaaat
    19501 caccccccaa attattgcca atatgattgt cgcaagaacc gtcaaatcca gcaagacagg
    19561 catcacactc accacatcca tgtgtaaaag ggacaatcac aaaatcacct ggtttcaccg
    19621 tcgtaatggc ttccccagct tcttcaacaa tcccaatcgc ttcgtgtcca ctatttttgt
    19681 gtccagcttt cgtttctgga ttacggtacc tccataaatc tgaaccacaa acgcacgcac
    19741 gaaccacacg aataatcaca tcatccactt ctattatttg cggacgttca atgctagcaa
    19801 gtccaacctg acctgccttt gtatatactg ctgatttcat ttaaaatttt ccttccttat
    19861 aaagtttaat tttgagattt aaacgattta aagctgtctg tagctgagac aagcgctcct
    19921 ctaatttttg cttttcctct tctaaaatac caagcctctc ctctctcgtt tcatctccct
    19981 tttggtagag cgacatatag tcaactaaac tatctacaga tacacccgcc gaacgaaaac
    20041 acttaataaa ttccagcgct tcgatatcct gatcttgaaa atcacgaatc ccagtagcag
    20101 tacgagtaat cggtggcaca agaccaaccc gttcataata ccgaatcgta tccgctgaaa
    20161 ttcccaacaa atcactggca gatttaatat tcacacgcta acctcctctc atttcattca
    20221 ctgaattcac gctattctaa ctcacttatc ttattatata actttgagtt gactccaagt
    20281 caagcatttt gtatctaatc gaaaaatttt tatcattaac aatcatatat ccggagttta
    20341 tacaatgctc ttctcttacc aatttttgaa atatttaaat aataaagaaa ttactctatt
    20401 ccaaataaga tagtacccaa gtatttttca cttttttcta atgttgatgc tcataactcc
    20461 gttccaagtc acacaccttt cgcttatggg tttggtgagt ttctaggtca gcatcaattt
    20521 caatggtaat attttgaaaa ccacaatctt ttaggaaaat tcgaatagac tctttacaag
    20581 tttccatatg ttccatttct tttagacaaa catggacaat ggcatttttt tccaaagcat
    20641 ccatagtcca gagattaagc tgattaaggc tggccacatt gtccaatcgc tccaggccac
    20701 tctttacttg cttgatatca agaccttctg gcacagcatc caagaaaatc ttgagtgtag
    20761 accaaaaacg tggaagggct tttgaaagaa taaagaaaga aatgacaagg gacaaaagag
    20821 gatctaggat ataccagtcc gtaaatcgaa gaacaatcgc catcaggata acagctaccc
    20881 accctagcgt atcttccaga aaatgcagac tcagaataga ctcattcttt gtctttccct
    20941 taccaaccac cagactcgct aacagattga tagtaatcgc aataattcct aaccagagaa
    21001 tcccctcatc attgactggt tgcggatgca aaatcttcgt gacattttcc aaaatgacta
    21061 gaacagagcc cgttacgaga atcacagctg ttaccaaggc tcctagcagg ctaaaccgct
    21121 tatagcccaa ggtgtactga ttgtcttctt cacgattgga gattgtttct agaaaagctg
    21181 atattccaat tgcaatcgca tctcccaagt catgcacaga gtcagcaaga acagcgctag
    21241 aaccaaatac tccacctgca ataaactcaa caatggcata agtcaaattt aagaaaaaag
    21301 ccacccaaac agcatatttt gccttcatat ttctcattcc tttgttataa tagatttatg
    21361 aacaccttgt tcattatcat tatccactaa aactcaaaga aaggatagaa gcaaaacgtc
    21421 agctttattc agttctgaac aatttgcctc aagtgtccat atgactaaca ttgaccgccg
    21481 tatcagcaaa accaaaaaag ccatctatca agcttttata caacttttga atgctaaggg
    21541 ctacgaggcc actactgttc aggatatcat tgatctcgca gatgtgggac gatccacctt
    21601 ttactgtcac tatgagagta aggagcttct tctggaccag ctctgccgct acctcttcca
    21661 tcatcttttt gaaagagagc aagccatttc aaccgaagat tacctagccc acctctttct
    21721 gcattttcag aaaaaccaag accatatcac cagtctacta ttctccaaaa atgactattt
    21781 cctccgtcaa ctacagagag agcttgagca ccacgtctat cccatgctgg ctgctgattt
    21841 gaagcaaact cacccaagtc tacctacttc ttacctccaa cacttggtcg tgtccaactt
    21901 tatcgagaca ttgacctggt ggctcaaaaa aggtcaagat ttcacagacc aggaagttgt
    21961 ccaattttat ctagaccttc tcattcctaa aaattgaata tagagtaaag cttcagttgt
    22021 cttatttcta ggttactgag ttttttatct tttcaacaac aaaagaggac ccgccgatcc
    22081 tctttttcat actataaatc cttgattatc aattatatct gttttaatcg aaatctcaaa
    22141 acagcacttt caaacatctt ttcctagtta agtaaatcag tattctgctt agctgccttg
    22201 ctccattgat accaaccaac tagactgtta atgagataaa ttagatattt cccttgaatt
    22261 tgcaggcttt ctccccacca gagatagatt gaaaagacat tggtagccgc ccagaatatc
    22321 cactgttcac ggtaaacagc tgtcatgagg atttgcccta ccccattggt tgcatctgtg
    22381 attgaatcac gatagggacg attggcacca atagactgat aaatgaagcc aaaggccaac
    22441 caccaaagca cactaatgga aagatacttt gtccagccct tgccgtccag tttacgcgcg
    22501 acaaactcct gcttttcctt cttaaaccgt gcctgataaa tccaaactag aagtccaatt
    22561 ggctgcatga ctgtgaagta aagtgtcgtc agcacctcac cataaaagcc tttctgtagg
    22621 gccaaaataa ggtaaataac agagttaatc aagccaaaaa gataattact tgctcgacct
    22681 tccgatacaa agattacaca gataatccct gtcaagctac aaatcatccc aatccagtca
    22741 acaatacgat gttcgtaaac caactccagc cagagaggaa aacttcctaa aaccagcaaa
    22801 taaatccact gggcaaaact acgatgggca aagaggtcat cccagatagc cttcatagtt
    22861 cctgaaaatc ctaaatcagc catagccgca accatacgac ggtaaccacc tgacatttca
    22921 cctagggttg ttttgatatt ttcaattttc ttttgcaaat aagtatgcat catttctcct
    22981 tttgttttta aagagccgtg tctggataga ctttcggacg caacgctcta ttagataatg
    23041 aactgcctat acacaagatt tctaacctta gtcgacatga gctgaaaccc cttatttgtt
    23101 aagtagttca caaaatatta tacacctatt ttatgaatag tcaactgtct ttacagtaaa
    23161 attttctctt tctttccatt ttaagtgaca ttcagtaatt ctcacatcaa aaaagcccag
    23221 acgaaattgt ctgagcattc ttttatctag tcgtttaagg aagttgagtt cagtatgttt
    23281 aaagtctctg tcccatcatt tcttcaacaa accttgttct tggagaaact ccttggctac
    23341 ttgctctgct gacttgcctt cgacacccac ttgatagtta agttggctca tctgactttc
    23401 tgtaatctta ccagccaatt tattaagaac tctttccaac tctgggtgtt tcttgagaag
    23461 agcttctttc atcagtggtg ccccttgata aggtgggaag agttgcttgt catcttccaa
    23521 gacctgtaaa tcataacgct ccaattccgc atcagtcgaa taggcatccg tgatttgaat
    23581 atcccctgac tgaattgcct gataacgaag agctggctgc atagtcgcca cattgagatt
    23641 gagaccatac attgattgca agcccttatt tccatcttca cggtcgttaa actcaagcgt
    23701 aaagcctgcc ttcagctgcc cttccacttt tttcaaatcc gaaatggtct tcaagccata
    23761 ttcttgagca atctttttcg gaacagctac agcataggta ttttgataag acataggttt
    23821 gagataggct agataatcct gcttggcaat gccatcacgc gccacttgat aaacctgctc
    23881 tggctcatga ctcaccttgg gtgatggttg aagcaaactt tcagtcaccg taccggtaaa
    23941 ttcaggataa atgtcaatat cacctttttt cagagcttca taaaggaaac ttgtcgtccc
    24001 aaaattcggt ttaacagtcg cagtcatgct ggtattttct tcaatcagca atttatacat
    24061 attagccaaa atttctggtt ctgggcctat tttcccagca ataatcaagt tttccttctc
    24121 tttttgaacc aaaagagctg gactataaga cagacccagt aataaagcca ccaaggcaaa
    24181 accagagaaa atcgtccgca atttcgcttt ttccattact tttagtagga agttaaaggc
    24241 aatagctagc actgcagaag aaagtgcccc aatcaaaatc agactggcat tattacggtc
    24301 aattcccaac aggataaagg aacctagtcc ccctgcccca atcaaggtcg ccaaggtagc
    24361 cgtaccgata atcaagaccg ctgccgtccg aattccagac atgataacag gcatggcaag
    24421 tggaatttca aatttcttga gacgttccca tctggtcatc ccaaaggcaa tcccagcctc
    24481 ttgcaggttc ggatcaattc ccttcagccc agtgatagta ttttgcaaaa tagggaaaat
    24541 cgcataaatc actagagctg tcaatgccgg taaagtccca attcccatca agggaataaa
    24601 gagtcccaac aaggccagag acgggatggt ctggaaaatc cctgcaatct gtaagaccca
    24661 atccgccaac ttctcatgat agcgaagaaa aacagccaag ggaatcgcaa gcaaaatagc
    24721 tagtaacaag gtcaaaagtg acaactgcaa atgttgagat agagctgtca accaatcact
    24781 aaaacgatcc tgaaaagttg caattaaatt agtcatgaac actacctcca aacaagtctg
    24841 ctacaaagtc tgttgcaggc gcttttaaaa ttgtctcggg attcgctacc tggcgaattt
    24901 ctccatcctg caagacagca atacggtccg ccaacttcaa ggcttcatcc gtatcatggg
    24961 ttacaaaaat cgttgtcatc ccaaactctt tatgcaattc ttttgtcaga acctgcaact
    25021 gttttctcga aatagcatcc aaggccgaaa agggttcatc catgaggaaa atcttgggct
    25081 gaccaatcat agctcgaaca ataccgaccc gttgctgttc tccaccagat aattcactag
    25141 gtaagcgatg cccatactcg gctactggta aaccaacctt agccaaaagc tcttctgttt
    25201 tcttcgtaat ttcttccttg ctccacccct tcatttcagg aatgagagca atattttccg
    25261 caactgttag atttggaaaa agagcaatag cctgtaaaac ataaccagta gaaagacgaa
    25321 gttcacgctc atcatagtct ttgatgcgct tcccatccat ataaatattt ccatcagttg
    25381 gttccaaaag acggttaatc atcttgagca tggtcgtctt acctgaccca gaaggcccta
    25441 ctaaaaccat aaattcccca tcctcaatct gtaagttgac atctctcaag acatcctttt
    25501 ctgtgtagcg cagtgctaca tttttgtatt caatcattct ttgtcctcaa tttaaaactt
    25561 ccctcgatta gtcaagtctt ctaccttagg catgacttcc ttattatccc aatgctccac
    25621 aattttcccg ttctctaaac ggaagatatc gtactgggca taagcaacgc catcaatctg
    25681 agtctgacca tagctaacca catagtttcc ttgtcctaag agttggaaaa caaagtcaaa
    25741 agtgacacta tattcagcca catagttttt ataagcagca cttccttgtc caatctcatg
    25801 attatgctga atcaaatcgt ctgccacata atcactccac tgctctagct ccccattttg
    25861 gaaaatttct gtcaagaaac ggcgaaccag ctttttattt tctgctttct tatccaaatc
    25921 cttgatttca aaatctccaa aaatttgatc tagttggtca ttttcaggtg ttcgatagta
    25981 gtcaatgaca tcccaatgct caacaataca accattctca tccgcacgga aagtatccgt
    26041 cgtcacccat tgagcttctc caccattcag atattgatga acatgaacaa agaccagatt
    26101 gccatcctca atggtgcgga caatcttaat ctgacgctct ggatgacgct caaagaaatc
    26161 tgcaaagaag gctgcaaatc cttctttccc gtcaggaaca cctgtcgaat gttggatata
    26221 ggtatcccct acagactggg cttgagcctc agcaactcgt ccgtcttgaa tggcatggat
    26281 gtataggttg tgagcatttt tcacttgttg tgacatattc taaacctcat ttcccttctc
    26341 tttcaaattc gccaaaattc tttcttgaaa accttcaaat tggtgaattt cttcctctga
    26401 aaatcctttg taaaagatag tatccaattt ctgactgaca cgatgcccca cttctttctg
    26461 gaacttgcct aactccgtta aaactaaata cttcttacgc ttgtcttttc cacacggact
    26521 aacaattaca agcttttgtt cctctagctt ttttatcata gtcgtcagcg tattattcgc
    26581 aagcccagtc gcaagcgcga tatctgtcgc agttgcgcag ccagtttcac tattccataa
    26641 aaccgctaaa atcttgccct gttcacccct ataaagagcc tcaggatctt gactcagtaa
    26701 cttttgaaaa atccgcccat tcaacaaacg aatatgatgg gctagcaaat gactatcttt
    26761 cataacacct ccaatttatt tcgatatcga aatgaataaa ataattgtaa cactcatcgt
    26821 tctaactgtc aactatttcg atttggaaat aatttttgat aattatccac accaccatac
    26881 tccggctcaa ctaactttta acgagagttt ctaaactcct tcgtcctcca gtctacaaaa
    26941 gccttccatt cgtactatcc tatattttat gaggggacac atttttccta tcagaccatt
    27001 tattttaaag atagaagtaa atcataattg cttccatctg ttcttttata gtatattgaa
    27061 gttagactag agcactgtat cttctaaaac attgatagaa agcgatttga atttcccaat
    27121 caatttgttc gtatttatag cattttgaaa ctagaatagg acaccatgac tgctaaaaga
    27181 tttctataaa ttcatttgat tttcctaatc aatttgttca tatcttattt cattccgcta
    27241 taatttcacc ttaccctatc tttttcgtag cacccttcaa acagcctatc ccctaccgtt
    27301 tgacgattcc tcacttcgct ccacttccat tacagaagtt tcttcactac tatgggctcg
    27361 gctgacttct catgattcct tgttactact atttgaacgc tcacgagata gatcttacaa
    27421 aaaatgcttt gatccacaat ggaatcaaag cattttaaag agttcctcat acataagcgc
    27481 agaagtcgca gttcctctgt acttggcttc ttctcttttg ataaagcgag ccaagttgag
    27541 caactcaggt gctggatgtt tgggatttag gagcaattca cgattgacca ggcctgagag
    27601 acgaactgcc tgcaattgct catttgtagt aggcagtttt ttagcagtct ctaggagagc
    27661 agcaactaaa tcttcactca aatcatgtcg agcatgattg taaagatctt ttataaggct
    27721 ttctaggttt ggttctgcca tccctaccac ctcccttatg gtttaataat gtttaatcaa
    27781 atcaaccgtt gaacgatcca atttcttcac caaggcttgt aagaaagctt gcgcttctag
    27841 gaagtcatcc attgcataga gggtttggtg agaatggata taacgagcgc agacaccgat
    27901 agttgttgat gggacaccac catttttcag atgagctgca cctgcatctg ttccgccttt
    27961 accacagtag tattggtact tgataccagc ttcttcagcc gttgtcaaaa gaaaatcctt
    28021 catccctggg agaagcaagt gacctggatc atagaaacga atcaaggttc catctccaat
    28081 cttgccttga ccaccgtaga catcacctgc tggtgagcaa tcaactgcga ggaagacttc
    28141 tgggtcaaac ttggttgtag aggtatgagc gccacgcaga ccaacttctt cttggacgtt
    28201 agaacccaga tagagttcat tgccgagttt ttgacctgat aaagcttcag ctagctcgct
    28261 taccatgagg acaccgtagc ggttatccca agcttttgag atgatatttt tttcattggc
    28321 tgtcaaaatt gcagaactat ctggtacgat ggtatcacca ggacggatgc caaaactttc
    28381 tgcctcagcc ttgtccgcaa aaccaccatc aaaaacgata tcggcaatgg ctggcatggt
    28441 tggtcccccc tttccacgag tcaaatgcgg aggaacagaa cctgaaatca caggaatttc
    28501 atgaccatca cgagtcaaga gtttgaaacg ttggctgcta accaccatgg ggttccagcc
    28561 accgatttct acgacacgga aggtaccatc tggcttgatt tcgctgacca taaaaccaac
    28621 ttcgtccata tgagaagcga ccaagacgcg cggtgcatcc acagcttctg aatgtttgat
    28681 accaaaaata ccacccaagc catctgtcac cacttcatcc acatgcggtg tcaacttttc
    28741 acgaagataa gcacggacag gcgcttcatg acctgagact gcagcaagtt ctgttacttc
    28801 tttaattttt gaaaataatg ttgtcatttc agttccttct ttctttcatc cattttacca
    28861 ctttttatag gagaaggata gtgggaaggt ggatttctaa gttagtatct tagtcctgct
    28921 ctatcttaga aaaggatagt attctcttgc atgtagtgca aaatctagta aacattccaa
    28981 aattaactcg aatatttatt tccaaacaaa aaaacaatac accatcaaag ttgtttgtat
    29041 ttttcatgaa atttacagaa aatagttgac ttccctttct tctttcttta aatatatagt
    29101 tggttgagtt tggaatagta cgctgtagct gctgaaacat ttctagaaat taatttgact
    29161 ttcctaatag agttgttcat atcttatttc aatttactat agtacaaaac tagaaaagga
    29221 aaaaatcatg accaaaacaa ttcttgttac aggtgggact agctacattg gctcccatac
    29281 cgttaaagct cttttaaatg ctggctataa ggtgcatgtc ctcgataatc tctccacagg
    29341 aaatcgagcg gctgtggata gtcgtgctag ctttaaagaa ctggatgttt atgatgctag
    29401 cgccttaaag gcttacttag aagaaaatca gattgatgct gtcctccatt gtgcaggtga
    29461 aattgttgtg agcgaaagta ttgaaaatcc aagtaaatac ttcactgcca acgttgctgg
    29521 tatgaaccaa gttctcaaag tcttatctga agttggcatt caaaaaatca tgttctcttc
    29581 gactgcttct ctctatggaa ataactgtat tgacaagcca gtgactgaag ataccctgct
    29641 cgaccctgtc aacccttatg cagagacaaa actgatgggc gaacgaatga tttactggat
    29701 ggccaatcgc tacgactgga aatatgttat tttccgttac tttaatgttg ctggggctga
    29761 aatggatgct tcaaacggtc tgcgtgtgaa aaatccaact cacatcattc caaatatcaa
    29821 caagaccgca ttgggacaaa atgatagctt aaaaatattt ggagatgact acgatacacg
    29881 tgacggttca tgtattcgag attacattta tgtcttggac cttgcatagg ctcatgttaa
    29941 aggaatgaac tacctatttc aagaagacag ttcttctcaa atctttaact taggaactaa
    30001 aaaaggctat actatcaaag aaatctttaa aactgctgaa gaattactaa attaaaaaat
    30061 cccacacgag attgttgctc gacgtgctag tgacccagcc agtgtcctag cagatgcttc
    30121 aaaagctaaa caatatcttg attggacggc tagctactcc ctcgaagata ttattttatc
    30181 agattaaggt aaaaacattt tagaatagga taacaaagga gaaagtcttg ttgctctctc
    30241 cttgttgtat ttattaaatt gtcagaaatt ttattgacag attagaaaaa taatgttaca
    30301 tttatatccg cagatatctt tcgataccaa atctacatga aaggacgggg tatgaaactt
    30361 tctcattatt taattggctt acttctactc ctagtctttc tctctattag cattgggacc
    30421 agtgattttt catggggaaa gctatttgat ttcgaccagc agacatggct cctctttcaa
    30481 gagtcccgtc tcccaagaac tatcagtatt ctcctgactg cctctagtat gagtatggca
    30541 ggccttctca tgcagactat tacccaaaat cagtttgctg caccgagtac agttggaacg
    30601 actgaagccg ccaaactggg aatggtgctg agcctttttg tctttccatc ggctagtctg
    30661 acccaaaaga tgctcttcgc ttttgtttca tccatcgtat tcaccctctt cttcctagcc
    30721 tttatgacca tttttactgt aaaggaaagg tggatgttgc ctctgattgg gatcatctat
    30781 agcgggatta tcggctcagt cacagaagtt atcgcctatc gtttcaatct ggttcagagt
    30841 atgactgcct ggacccaggg ctccttctcc atgattcaga cccatcagta tgagtggctc
    30901 ttcttaggcc tcatcatcct gataaccgtt tggaaattat cccaaacctt caccatcatg
    30961 aatctgggga aagaaatcag cgagagtttg gggatttcct actccctact tgaaaaactg
    31021 gccctctttc tggtggcgct aacgacaagc gtcaccatga ttaccgtggg tggcctacca
    31081 tttctcggag ttatcgttcc caatcttgtt cgcaagcgct atggagataa tctaagtcaa
    31141 accaaactca tggtcgcact ggttggtgcc aatctagttc tggcttgcga tatcctatcc
    31201 cgagttctga ttaggcccta tgagttgtct gtcagtctct tgctaggaat catcggtagt
    31261 ctcgtcttta tcctacttct ctggagaggg ggacgaaaag atgcagacta aaagcaaaca
    31321 taccaagctc ttctggattc tcattattct tgccatcgga gcttgtcttc tctacttttg
    31381 gcccatcact cacttgtcag cctttgcttg gaagttgcgt tcccaaaaga tcatcgttta
    31441 tctcttggta gccatcgcga ctgggatttc gaccattagt tttcaaaccc tgacggaaaa
    31501 tcgcttcctg acgcctagta ttttgggaat tgaatccttc tacgtcctac tacaaaccct
    31561 actactggtt tttgaaagca agtttcttca acttggcaaa tcccctatct tagaattcct
    31621 agtcttactt cttgtccagt ccctcttctt tctcacctta caaggttact tgaagacact
    31681 gatgaagcaa gacctggtct tcatcctgct gatctgtcta gcgctcagaa gtctctttcg
    31741 aaatatcagc accttccttc aagtcctaat ggatccaaac gaatacgata aactgcaaaa
    31801 tagtcttttt gcctcctttc aacatctcaa cacttccatc ctagccatcg gttctctgat
    31861 catcctcgct ttgacaatct ttttctttcg aaaagcagtc gttctagatg tcttgcacct
    31921 gcaaagagaa acggctcaga tattgggact cgatgttgaa aaagaacaga aagagctcct
    31981 ctggggaatc gtgcttttga cctcaacggc cactgccttg gtaggaccta tggccttctt
    32041 cggctttatg ctggccaacc tcacctacct gattgtcaaa gactatcagc acaagttact
    32101 ctttatagtg gccattctgg ttggatttat tagcttaacc ttggggcaag ccttgattga
    32161 acgagtcttt gcactggaaa ttcgtatcag tatgatcatt gagagtgtgg gtggcttctt
    32221 attctttatc ttactatata ggaggtctcg tcagtgaaac tggaaaacat tgacaaatcc
    32281 attcaaaaac aggatatttt gcaaggcatt tcgcttgaag tcagtcctca aaaactgaca
    32341 gcctttattg gtccaaatgg tgctggaaaa tcgactctcc tctccatcat gagcagacta
    32401 accaagaaag atcagggagt tctcagtatc aaaggacgtg aaatcgagag ctggaattcg
    32461 caagaactgg cccaagaact aaccatccta aaacagaaaa tcaattacca agccaaattg
    32521 actgttgaag aactggtcag ttttggacgt tttccctaca gccgaggtcg acttagatca
    32581 gaagactggg aaaaaatccg agaaactctg aactatttgg aactgaccaa cttaaaagac
    32641 cgctacatca atagcctgtc aggggggcaa ctccagcgcg tctttatcgc tatggtactg
    32701 gcccaggata cggactttat cttgctggac gaaccactca acaatctcga tatcaagcaa
    32761 agcgtcagca tgatgcagat tcttcgacga ctggtggagg aactcggcaa gaccattatc
    32821 atcgtcctcc acgatatcaa catggccagt cagtatgcag atgaaattgt cgccttcaag
    32881 gacggccagg tctttagcaa gggaagcacc gatcaaatca tgcaggctga cctgctcagt
    32941 caactttatg agattcccat cacgctagct gatatcaatg acaaaaagat ctgtatctat
    33001 agctagtaac ataaaagctc aagttagaga accttcagtc tcttagtcaa taagattaag
    33061 agactcccta aatcgttatc acattttaaa aaggagaaat tatgaaaaca tcccttaaac
    33121 tttatttcac tgccctagtg gccagcttct tgctcctact tggtgcatgt agtacaaact
    33181 caagcactag tcagacagag accagtagct ctgctccaac agaggtaacc attaaaagtt
    33241 cactggacga ggtcaaactt tccaaagttc ctgaaaagat tgtgaccttt gacctcggcg
    33301 ctgcggatac tattcgcgct ttaggatttg aaaaaaatat cgtcggaatg cctacaaaaa
    33361 ctgttccgac ttatctaaaa gacctagtgg gaactgtcaa aaatgttggt tctatgaaag
    33421 aacctgattt agaagctatc gccgcccttg agcctgattt gattatcgct tcaccacgta
    33481 cacaaaaatt cgtagacaaa ttcaaagaaa tcgccccaac cgttctcttc caagcaagca
    33541 aggacgacta ctggacttct accaaggcta atatcgaatc cttagcaagt gccttcggcg
    33601 aaactggtac acagaaagcc aaggaagaat tggccaagct agacaagagc atccaagaag
    33661 tcgctactaa aaatgaaagc tctgacaaaa aagcccttgc gatcctcctt aatgaaggaa
    33721 aaatggcagc ctttggtgcc aaatctcgtt tctctttctt gtaccaaacc ttgaaattca
    33781 aaccaactga tacaaaattt gaagactcac gccacggaca agaagtcagc tttgaaagtg
    33841 tcaaagaaat caaccctgcc atcctctttg tcatcaaccg tacccttgcc atcggtgggg
    33901 acaactctag caacgacggt gtcctagaaa atgcccttat cgctgaaaca cctgctgcta
    33961 aaaatggtaa gattatccaa ctaacaccag acctctggta tctaagcgga ggcggacttg
    34021 aatcaacaaa actcatgatt gaagacatac aaaaagcttt gaaataagcc acttcaaact
    34081 caatttaata ctcaatgaaa atcaaagagc aaactaggaa actagccgca ggttgctcaa
    34141 aacactgttt tgaggttgca gatgaaagct gacgcggttt gaagggattt tcgaagagta
    34201 taaaattttg acattctaaa acgcatccta tcaagttcac tcaaacctga taggatgcgt
    34261 ttttatcatt ttgaaatcta tttaccccac ctcattcccc ttcttgattt cgtttaaggg
    34321 aaaagtggtc tggaacgggg tccttacctg ttttcgacca gggatgacaa cgtaaaatcc
    34381 gagccaagcc catcaatacc cccttaaaac catgtttttc aatagcctga atcatgtagt
    34441 tggaacaagt cagctcaaag cgacaagagg gtggaaagac tggtgagata aaacgttggt
    34501 aaaagcgcac aggcgctatt aaaattcgtt tcattatttc ttagttacag ccatggtgtg
    34561 tagttggctg atttcttttt tattaagacg acgggattct cctggacgga gtcctgtcaa
    34621 gtctaggtgt ccgaaacgag tccgagacaa cttatctact tggagaccaa cagcttcaaa
    34681 catcttttta acctgatggt tacgcccttc atggatggtc aactgcacca cagagcgatt
    34741 tttgactggg tccactttga gaatttcata aacagctggc ttggttttct taccatcaat
    34801 ctcaagacca cgggtcaagg ggcggagatt gtccttattg gccacaccct taacacgcgc
    34861 gacataaacc ttgtcaatct cattacgagg gtgaatcatc tcgtctgtaa agtccccatc
    34921 attggtcaaa atcaagacac ctgatgtatc ccagtccaaa cgacccacag ggtaaatacg
    34981 ctctttgaca ttgggcaaga ggtcgacaac cgtcttgcga cccttatcat ctgtcacact
    35041 ggaaatcaca ccgcgtggtt tgttaagcag atagtagacc ttttcttcgt tgtagatagg
    35101 ttgaccttca acttcgacct tgtcgcctga cttgatagtg gttgctagtt cacgcaccac
    35161 ttggccgtta accgtcacca agccttgctt aatcagctct tctgcttttc tcctactggc
    35221 cacacctgcg tgggcaatat acttattgat tctcatcttc ttctatcctt tcaccaaata
    35281 attggctttc ttgggcttga atctcaagct catcaatcac tggtaattct tctaaatggt
    35341 ttatccccat gtaatctagg aaataatccg tagtcacata gaggttgggg cgccccaata
    35401 cttccttttt cccgtcttcc tttatcaggt caaaagcctg caactttgcc aaggctccac
    35461 tcgagttaac tccacggatg gcatcaattt ctatccgcgt aatcggctgt ttgtaggcaa
    35521 taatggacaa ggtctcaagg gcagcccgag acaagctctg gttgataggc gccttagagt
    35581 attccttcaa aatctctgca aattgagact tggtcaccaa tctataagca ccacttgtct
    35641 caatcaaagc caaactggaa tctgggtcct tttcatactt ctgggctaat tttcctaaac
    35701 tctgctggat gcctgtcggt ggcagagaga ggagttcagc taactggcgg acccgaatcc
    35761 catcttcacc cgctacaaac aagagcgctt ctatttttgc taaagtactc atctttcctc
    35821 tctatcaagt ctagctttga ggcacttgac tttcttcctt cttttccatg agatagatat
    35881 ctccaaaact ctcctcttgc acgaggatca gctcctgggt tttgattaac tctagggttg
    35941 ccaaaaagag ggtgatgacc tcttggacat tctgggcttc cttgaacaaa tcctgcaagc
    36001 gcaattgatc tcgtccaatc aaggactctt tcacgataat catcatgtcc tcaatcttat
    36061 actcatcccg caagatagtc gtgtgatttt gtgcaaactc ctcttttttc ttggctagga
    36121 tatttgaaaa agccaaaaag aggtcaatgg tcgtcttgtc atgcacaagc tccgcatctt
    36181 cgtaaatcaa ctctgtcggc gctttggaat aatactgggc ccgttcttgg tgcttggctt
    36241 ccaagtgctc acccaagagc ttgaacttgc gatattcttc gatttgagag aggaggtcct
    36301 gctccaggtc atcccccaag tctgtcactt ctgctacctt cggaaggagt ttacgactct
    36361 taatcagcat gagctgacta gccatgacca tgtactcacc cgtcacttcc agacgcatgg
    36421 cctgcagggt tgaaacatag gctagatact gttcgatgac ttccgtaatg ggcacatcgt
    36481 agatatccat ctggtactta gaaaccagat gcaagagcaa gtccaggggg ccttcaaaat
    36541 cttttaattt aatatccatt atctatattt ttctaaggtc aggactgttt ttaatcctaa
    36601 tttttttgca atttcgtaca aatcgacctt gttttctatt tgtcttagaa taaactgttc
    36661 acgtaagact tgagctgata aggatggaaa acctttctcc ttgacaaaag attctaactg
    36721 acgaaaggcc cactgacgag aatagggttt ctctcctctt tcaaaaagat aggtctgccc
    36781 catcaagggt tccaattctg aaagcaaggc cgtgggaatg gtgacaatcc tctgttggga
    36841 agccttgctg attcgcaaca cctgaaaatc cagattgatg tccgcaacct tgatggctaa
    36901 aatctcactg ggcaagagcc ccatttctag gattaagagc gctagcaagc ggccctctgg
    36961 atggtcgctt tcctgccaaa aagagtctag gtatagaatc tctggctttt ccgtcttctt
    37021 ttcagcttgt ttggctaatt ccaagcggta aaagctgtcc acctctcctt tttgatagag
    37081 aaagtataga aattggttac aggccgaaat ctttcgcttc tgggcgctga tttttagatt
    37141 ggctagctgg gcttggtaaa tcttgagact ggtctcagaa atccgctcac ctaccatgtc
    37201 taaaaattgc tccaaatcat acttatagga ctgcttggaa ttgacagaca agccctgctt
    37261 ttcctctaaa aaggctgaaa tcctgtctct cattggcatc gaatctcata ttccttacta
    37321 aagtcatgca agagggcatt aacggccttg aggatggact tacgcgtaat aatcccttgg
    37381 aaaataccct ctgcatccac aaccggtaag aaggactcat ctactagctt gtgcaagacc
    37441 tccgtaatgg tgaaatcagg cgaaacaacc gctacgtccg tttttgtcat atgaacgata
    37501 tccgtatccg ccatgatttc ttggctcaag tcatgctcca tctgataagc cataatatct
    37561 ctgagtccaa tcgtcccaac aaactgtttt tcatctgtca caacgggaac acgggtatag
    37621 gtcatctgac tgagcaagag ggtcgcatga tccgcattgt gggtatcaat caacacagct
    37681 agatttttag caggggtcaa aaaagtttcc tcctgcccca acaagaaagt ctcaaactcc
    37741 ttggcaatca tcggctaaac tccttggaca aacctggata cacctcgtga tctcgtgtca
    37801 aaaagtccac tttgaagtaa ctatcatcaa tctccacacg agcatagaga cattctctga
    37861 tggtacctcg tggttgactg atagaacctg gatttagaaa gaggatcttg ccttccaacc
    37921 aagcacttgg cacatgcaag tgaccataga ggcagatagc ggcctcttcc tcctgagccc
    37981 agtagtccaa cttttgaaag ttgaaattga tgtcaaacaa gtgaccatga gtttggataa
    38041 tcttggtcga accaagctca gtcaccagac gttctgggta gccggcgtag aagtccatgt
    38101 ttcctttaac aacgcggatg ccctcccaaa gtggagaatc cggacgtagt tcagaatcgc
    38161 cgttatgaaa aacggcatcg actttgccca catagcgatc acggacttct tccacaatca
    38221 agctatcgcc atgggaatcg ctcattacaa tgatggtttg ctttgccatg atggaaatac
    38281 ctccaaaagt ttcttaacgg ctaaggcacg gtgagattga ctattttttt cttccagggt
    38341 taattcagct gatgacttac ctgtctctcc tacaaggaag aggggatcat agccaaagcc
    38401 attttcaccc ttaggttcaa agttaatata acctgaccag tctgcttcaa caactaaact
    38461 ttccttattt gggctggcta cgactagggt tgtgtggaac tgagccgagc ggtccttgag
    38521 ttcaaagacc atggccaatt cgtgcaagag tttggcatta ttttcacggt cagttgctcc
    38581 cacacctgcg aaacgagctg accagacgcc tggtaagcca ccaaggacat cgactttgag
    38641 accagaatca tctgccaaaa ccatcttgcc cgttaattga gaaatggttt ctgccttaag
    38701 gcgggcattt tcttcaaagg tcatacctgt ttctgctact tcaggcaggt cagggtagtc
    38761 attaagattt tccacatcgt agcctaactt atcaaagata gctcggaatt ccttggtttt
    38821 gccttcgtta cgagtcgcaa tcaacaaggt ttctctgacc ttgcttgtct caaagaaatc
    38881 atgaacattg accccttctt taggcaattc tacatacaag agttgcccat tttcaaccaa
    38941 gagcagggcc ccatgacgtt gaataacttc caagtttcgt cccatttctt gattaagcat
    39001 gtctaccaca aaggagaaca aacggtaggt agaaccatag cgtaaaacag taactgaaag
    39061 cgggaaacca tactcttcat ctccaaatat tttggagaat tcaaacagag gaaaatctgt
    39121 cttatagtcg ctcaaactgt taacgccacc aaaaatacta taagacccaa cataccaatc
    39181 ctggtcatcc ttatattcat aaattttatt tgtcataatt ctacatgctc cacataaatc
    39241 tctttttcca gccattcttc accaatttgt gcaaaacttt ggctactggc tgttgtgtaa
    39301 aaacggtgat ggagtggtcc agcatcgcga ccacgattga tttcaaaata attgagtaag
    39361 actgagatat cccgtacgca ctctgcccca ctatcgatga gctgaacctt tggccccatg
    39421 acattttgga taatagggcg aaggagtgga taatgagtac agcccaaaat caggctatcc
    39481 acctttccaa ccaagggacg cagggtttca tagaccacct tcttggtaac actggttgac
    39541 agggcacctg actcaaccaa gggagcaaac ttgggacagg ccaagctctc cacctgtaag
    39601 tcgggatcca gatcatggat tttctgacgg tatatgtctg attgtaccgt catgggcgtt
    39661 ccaatcactc cgattttccc accttgactg gacttgatgg ctgccgaagc tcctggcaaa
    39721 attacaccca agacaggaat atctagttga gccttgattt cttcccagac gaccgcagtc
    39781 gcagtgttac aagcaatgac aatcattttg acatccttgg tcaagagaaa gttgaccagc
    39841 tgccaagtat attcacgaat ttgctcagct ggacgggggc catagggcgc ccgcgccgaa
    39901 tctccaatat agacgatttc ttcatgggga agctggcgca tgagctcgcg cacaacggtc
    39961 aagcccccga cacccgaatc caaaaaacca attggtcgat tatccataca gtcttctttc
    40021 tagtcttttt tctgattgat cgttcattat gattccttgt actgaacgaa ctgaccgaca
    40081 gcttgataga atgtcaacta cctctctctt aatcataatc aaatatcaat agaatgcaag
    40141 gaaaaagtct catccctaaa ccataaccaa catagtgaga agtctggaac tttcatccca
    40201 gacttttcct tatttatttt ttctttttat tgttagcaag ggctgccttt tgttggcgga
    40261 tgatttggtg gtaaacttgt tgtaccttag cttcgcttgg tttttgacca tttgcactca
    40321 aaagagtacg aactgcttca gcattcaaac gtgggttgtc agcaaattct ttttcgattt
    40381 gcttacgaac caagtacatt cctccaagag ctcctcctag aaaagctagc acaatcaata
    40441 caattgctaa aagtaaatcc atcatttttc tcctgtttct ttttgagtcc ctttcattat
    40501 accacaaact agccaccctg accagtttgt tctacacttt cagggatgga atagacagca
    40561 aaaagaaccc tgcttttctg aaaaagaggt ctccttgcta agtttaattt acataaatag
    40621 ccagtgtttg atagggtttt agtagaattt tctcagacac ttctgcatct tcatagtttg
    40681 aaatcaaaat ctgtccattt tggtagactg ctggcaagtc gatttccact tctttagcat
    40741 aaaagttatt gagcactagt aacttttgat cctcaaactg gcgttcaaaa gcgtagactt
    40801 gtttgctatc ttcaaaggct ggtttgtaac ttccttctga aatgattggc atttccttac
    40861 gcagtcgaat caagtcttga tagaaggtaa aaatcggacc ctggatttca ttttctacat
    40921 tgatgtcttt ataggattta ccagctttca accaaggagt gcctgttgaa aatcctgcat
    40981 tttccgaagc atcccactgc atgggaatgc gtgaattatc acgcgactta gcttgaataa
    41041 tctggaaggc ttcttgctga ctctttcctt cttctaggag catctgatag gcattaagcg
    41101 attcgacatc cacgtaatca gccatagaat catagtctgg gtcaatcatc ccgatttcct
    41161 cacccatgta gatataaggt gtcccacgtg acaggtgaat gctggctgct agcatggtgg
    41221 ctccttcctt gcggaagttt tgaatatcga caaaacggtt caaggcacgt ggttgatcgt
    41281 gattgttcca aaagagggca ctccaaccgt ctttatcact catttcctta ccccaactat
    41341 ggtaaagact cttcaactct tcaaaatcaa agggagccaa ggtccacttt tgtccatcct
    41401 tatagtccac cttgaggtga tgaaaattaa aggtcatgga taattcctga cgatcaggcg
    41461 acgaatagag gacacagttt tccatggtgg tagaagacat ttccccaact gtcataaagc
    41521 tatcgtcaga tccaaaagtg gcttggttca tcatacgcaa atagttatga acgatgggtt
    41581 tgtctgtata agctggcttc ccttcatttt caggacagtc cactgaaacc tcgtccttac
    41641 caatcaaatt gatcacatca aatcgaaaac ctttgacacc cttgtcgcgc cagaaattaa
    41701 caaccttgaa aagctcctta cggacattgg gattgcgcca gttaaggtca gcctgggtct
    41761 catcaaatag gtgaagatag tatttcccag tatccccgaa aggcgcccat gcagaaccac
    41821 caaacttaga ctgccaatct gttggttggt cttggatgaa gaaaaagtct tgataatact
    41881 tatcaccagc tagggctttc tgaaaccatt catgctctgt cgaacaatga ttaagtacca
    41941 tgtccagcat aaagtcaatc ttgtgctctt taccgataca caccatttcc tcaaaatcag
    42001 ccatatcacc aaaaagagga tccactgcca tataatctga aatatcgtaa ccattatccc
    42061 gttgaggact tggatagaat ggattgagcc agaccatatc cacacctagt ttggctagat
    42121 agggaatttt tccgataatc ccacggaaat ccccaatacc attgccagtg gtgtctttgt
    42181 aagattttgg atagatttga tagactactt ttcctttatc aagtgtcatc tgtttctcct
    42241 tttctgataa aagggaggaa gcagtcttcc gtccctattt gtgctatttc aattatactc
    42301 aatgaaaatc aaagagcaaa ctaggaagct agccacaggt tgctcaaaac actgttttga
    42361 ggttgcagat agagctgacg tggtttgaag agattttcga agagtattag attcgtgtag
    42421 cgaccatgag agatgctcca gcttgaatcg ttgtcggata agttccggga atagtcgctg
    42481 tataagcatc ttggttggtg atgataacag gagtttctgt caccagacct gcagccttaa
    42541 tgacatccat atcaaaacga atcagttgct gaccaactgt aacgtgatct ccttggacta
    42601 caagactttc aaaacctttg ccatcaagac ctactgtatc cataccgatg tggatgagca
    42661 attcaactcc ctcgtcagag acaatgccga tggcatgctt ggtagggaaa agaaccgtca
    42721 ctgtcccatt aactggagag gtcaactcac cttggcttgg ttcaatgact agaccttgcc
    42781 ccatgacacc tgatgcaaaa ataggatccg tcgcttgact caattctttc acttggccag
    42841 ttagtgggct gataatttct accgaagtaa gttctactgg ttcatggttc acaaattctg
    42901 cttcttcttg agcaacgaat tctgcctgca agttcgtatc gccctctgtt tttgtaaaga
    42961 gaccagcctt gcggaagaag aaagtcaaga gcattggaac aacaatcgca actagcatag
    43021 ttcctgcaaa tggcagcatg tattgaggtt gaatagagag aatacctggc aaaccaccga
    43081 taccaataga agccgcagtt acattaaaag taacggataa catgcctgca agggctgaac
    43141 cagtcatccc agcaacaaat ggataaatat attttacgtt aaccccaaaa agagctggtt
    43201 ctgtaacacc gagataggct gaaatggttg caggaagtga aacctgagcc tcacgctcat
    43261 catggcgatg catgaaataa taggcaaaca cggctgagcc ttgagcaata ttagaaagag
    43321 caatcattgg ccatagggca gtgccaccag catccgcaat caattgtgta tcaatggcat
    43381 tggtcatatg gtgcagacct gtgatgacaa atggagcgta gagggcacca aaaattgcac
    43441 cgaagagcca tttaactgga ccagttaaac ctgccaagac aactgatgaa agtccttgtc
    43501 caattgtcca accgattggt cccaaaacag tatgagccaa aatcaaggct ggaatcaatg
    43561 acaagaaagg tacaaaaatc atagaaatga cttctgggat atgcttgcgc cagaagattt
    43621 caagataaga tagactcaaa cctgcaagca aggctgggat aacttgggct tggtaaccga
    43681 tacgattaac agtaaaatag ccaaaattcc aaacccagtt tgccgcgata tcagctgctg
    43741 gcgttgaagc aaccgcatag gcattgagca actgaggcga taccaaacag attccgagaa
    43801 caattcccaa aatttggctg gttcccatct tacgagaaac agaccaagta atccctactg
    43861 gtaagaactg gaagatagct tcaccaggca accagaggaa atgattaaca cctgcccaaa
    43921 actgagagga ttctgtgatg gtcttgccat ccaacatcga ccaatggaca ccttccaaga
    43981 cattacggaa accgaggatc aatcctccga ctatcaaggc tggaataatc ggagtaaaaa
    44041 tctccgccag agtggtcatg acacgttgga ccacgttttg attactctta gctgcagact
    44101 tggctgcttc tttggaaaca ccctcaatac ctgaaacagc tgtaaaatca ttataaaaga
    44161 tgggcacgtc atttccaatg attacctgaa attgacctgc atttgtaaag gttcctttaa
    44221 cagctggaat tgactcgata gctttaacat tagccttctt atcatttcct aaaacaaagc
    44281 gcatccgtgt tgcacagtga gttacggcag tcacattttc tttgcctccg attgcctgaa
    44341 gcagatcttt ggcttcttgt tcaaattttc ccatttgatt ctccttatac ttgtcaaagc
    44401 aagtttgatt tgataagtat attgtaaacg ataccaaact tgtctgcaac ttgtttggta
    44461 taaaaagggt gtttttttgt aaattttcta gcacttgtat ggtaaaatag atacaggtgt
    44521 tcattaaact agactaaaaa cctatttaag caggcaaaat gaagaaatac caacaattat
    44581 ttaagcaaat ccaagaaacc attcaaaacg agacttacgc tgtcggagat ttccttccta
    44641 gcgagcacga ccttatggag caatatcaag tgagtcgtga taccgtccga aaggccctgt
    44701 ctctcctcca agaggaagga ttgatcaaaa agataagagg gcaaggttct caagtcgtca
    44761 aagaagaaac cgtcaatttc cctgtatcca acctaaccag ctaccaagaa ctagttaaag
    44821 aacttggact gcgctctaaa accaacgtgg tcagtctgga caagattatt attgataaaa
    44881 aatcctcact gataaccggt ttcccagagt ttcggatggt ttggaaggtg gtccgccagc
    44941 gtgtggtgga tgatctggta tccgttctgg atacggacta tctggatatg gaactcatcc
    45001 caaatctcac tcgccaaatt gctgagcagt ctatctattc ttatatagaa aatggcctca
    45061 aactccttat tgattatgct cagaaggaaa tcaccattga ccactcaagc gaccgagaca
    45121 agattctcat ggacattggc aaagaccctt atgtcgtttc gattaaatca aaagtctatc
    45181 tccaagacgg gcgccaattt cagtttaccg aaagtcgcca taagttagaa aaatttagat
    45241 ttgtagattt tgcaaaacgc aagaaataaa agactgagac accagatctc agcctttttc
    45301 tttatcccaa aactaattca tcactgacca gactttggcc actatttttc tggaagagat
    45361 gcataaggtc ttcaactgtc agatgctttt tctcttctcc cttgacatcc accactatct
    45421 tgccttgata gagcataatg agacgattgc cgtactcaat cgcatgattc atatcatggg
    45481 taatcatcaa agtcgtccac tgatggtact ccacaatctt ttgcgtcaag tccatcacca
    45541 tttgactggt tttcgggtca agtgccgcag tatgttcatc caaaagcaaa agtttgagtt
    45601 tcaccagagc tgccatgact agggtcaaag cctgtctttg tcctcctgag agatattgag
    45661 tatctacttt taagcggttt tcaagaccaa tattcaactc cttcaaggcc tcttggaact
    45721 ggattctatc cttctccttc acgccccaac caagccctct cttttgccca cgtctcaagg
    45781 caatagccat attctcctca atcgtcaaac gagaagctgt tcccatctta ggatcttgaa
    45841 aaacacgggc aatatccttg gctctctttc tgacactcag atgtttaatg gatttccctg
    45901 ccagtagaag gtccccttcg tccaccgata gattaccagc caagatattc atcaaagtgg
    45961 attttcctgc tccatttcca ccaatcacag agataaaatc tccctcttca acctctaaat
    46021 ctaatccttt gaggacatgg ttctcattga ccgtccctgc ttcaaatgtc ttgtgaatat
    46081 tttcaagtgt taacaaactt gccataactt tctcctccta ttcatttctc aatttcagac
    46141 cacgaatctt taatctcttt tgcaatgctg gtgcaaataa aaccaaagcg agcaagattg
    46201 cattaaagag acgtaccaag ttttgatcta gatttggaat ttcatagata tttaaaataa
    46261 tcaaacggta aacaatagca ccgattccga tggataataa gcggcctcca atggtcaagt
    46321 cgtgtatcaa gacttccgag ctcaaaccaa caacaatggt tcctgtccca gaagtcacat
    46381 ctgaaaatcc atcattttgg gcaaacaagg aaccacatag ggcaatcaaa ccggttgaaa
    46441 tcatgtaacc aacaatcttc atggtgttta cattgactcc attggcctca ctcatcggga
    46501 tattgtcccc agtcgaacgc aagaccaaac caatctctgt tttcatcaaa agggtcaaaa
    46561 ccaaacaagt aagagacaga ctaaactaag tgagaaaaca gctccttcat ttgtcagacc
    46621 caagctcgcc aactgtttaa agacggttgc agaatctccc aaggaaagat tgggtacact
    46681 gcccatgatt ttaatattga ttgaatagag ccctgtcaag gtcacaatcc ctgtcaagag
    46741 agctgggatt ttcatcttgg tgtggagcat tcctgatata agacctgcta ccatacctgc
    46801 cagcaaagca agtaaagtcg caagccaagg atttgcccct gcctgtatct gtgatacaac
    46861 gattgctgcc cccataggaa aggctccctc agcagtcata tccgcaatat ccaaaatacg
    46921 gaaagtcaag tagaccccaa ttgccataat cgaccacaac aaaccttctg ataaactaga
    46981 taacacaaaa ttcatcttaa tcctccttat tccttgcctt ctaacttact aatatcaatt
    47041 cacaatgcat ctgccatttc ttgattagta tgcaattcca gtttttcagg caactcaact
    47101 gctatatttt ctggttcctc accttttaaa acacgaatca gcatgcgagc tgtctgtctt
    47161 cccaattctt cataattggt tccgtagtta tacaagccac caacagcaat catttctgtt
    47221 gaaccaccga atactggaac cttgtgttta atagaaacct gcttaactgt ttccatggtt
    47281 gacgaaatga tattatcagt tgggacaaaa accatatcca cctctgccat caaactctct
    47341 gctgccgcct tgacgttatt actgtccaag attgtttttt caacaacggt aaagcctttt
    47401 tctttcagaa atcgcttagc ttcatccttt taaacaaccg aattgggctc gctctgagta
    47461 tagaggattc caatcgtttt agcctttagc aacactttct ttatcaaatt gatttgggtt
    47521 gaaatggcat ctgatgactg atcgcttgtc ccagttacat tgcccccagg atgttctctt
    47581 gactcaacca acttggcact gactgggtct gttacagctg aaaagataac tggtgtcgtt
    47641 tgcgtcgtat tggccaagct ctgagcagaa ggccttgcga tggctagaac gacatcgctg
    47701 gattctgcta gttgctggga aatcgttttt agatttcttt gttctccctg tgcattttgc
    47761 aaatcaatct caatattttc cccctcaaca tagccttgtt tggccagctc atccacaaat
    47821 ccttctctag tagcgtctaa ggactggtgg gtaataaact gcgaaatacc gatacgaaag
    47881 acatctcttt tcttatctgc cttcaactga tgcaaactga tcagagaagt caagaggatc
    47941 cccactacca agagggggct agtgattttc gaacaacttt cataatgaaa ctccttctca
    48001 gaaaagataa aacaaaaaaa cgcctagata ctatctaaac gggtacttga aataacctaa
    48061 caagttacaa cttagctggt ccatttagat attcatctat atggaccaca atcaaaaatc
    48121 ttagccacat agatacaaac aaattgcttg aactgtttgc atccatggtg acatgtctac
    48181 aaacactatc taaagtagct aaagtaaaag ttttgattca tagttacggc ttgtctctta
    48241 ttcatttctt tttctgcttt cttttttgat ccttcctatc ctaccacctt tttcatcacc
    48301 tgtcaataat atttcaaaat tttttaaaat ttttcgaaaa ttctttcag
//
LOCUS       DAVBGY010000009        48285 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVBGY010000009 DAVBGY010000000
VERSION     DAVBGY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48285
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.009"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(741..1001)
                     /locus_tag="VQ620_000529"
     CDS             complement(741..1001)
                     /locus_tag="VQ620_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763209.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDITRLTASLLGDEKLSAITSSSA
                     VKADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
     gene            complement(1060..1611)
                     /locus_tag="VQ620_000530"
     CDS             complement(1060..1611)
                     /locus_tag="VQ620_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763210.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(1601..1798)
                     /locus_tag="VQ620_000531"
     CDS             complement(1601..1798)
                     /locus_tag="VQ620_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763211.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(1802..2386)
                     /locus_tag="VQ620_000532"
     CDS             complement(1802..2386)
                     /locus_tag="VQ620_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763212.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(2794..3219)
                     /locus_tag="VQ620_000533"
     CDS             complement(2794..3219)
                     /locus_tag="VQ620_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763213.1"
                     /translation="MTSRVFIDADCISAFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNYDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(3212..3706)
                     /locus_tag="VQ620_000534"
     CDS             complement(3212..3706)
                     /locus_tag="VQ620_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763214.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMVLGHYIKSTEQLLENNRISQGKYEELLLDAFRYDIVYGLDEEGE
                     LSFD"
     gene            complement(3752..3958)
                     /locus_tag="VQ620_000535"
     CDS             complement(3752..3958)
                     /locus_tag="VQ620_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763215.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(3945..4106)
                     /locus_tag="VQ620_000536"
     CDS             complement(3945..4106)
                     /locus_tag="VQ620_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2763216.1"
                     /translation="MNMINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(4471..5427)
                     /gene="lytA"
                     /locus_tag="VQ620_000537"
     CDS             complement(4471..5427)
                     /gene="lytA"
                     /locus_tag="VQ620_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HET2763217.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPDFTVEPEGLITVK"
     gene            complement(5803..7173)
                     /locus_tag="VQ620_000538"
     CDS             complement(5803..7173)
                     /locus_tag="VQ620_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET2763218.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYHLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            complement(7480..8646)
                     /gene="recA"
                     /locus_tag="VQ620_000539"
     CDS             complement(7480..8646)
                     /gene="recA"
                     /locus_tag="VQ620_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET2763219.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            complement(8701..9957)
                     /locus_tag="VQ620_000540"
     CDS             complement(8701..9957)
                     /locus_tag="VQ620_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET2763220.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNLLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQVDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGISSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(10041..11057)
                     /locus_tag="VQ620_000541"
     CDS             complement(10041..11057)
                     /locus_tag="VQ620_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET2763221.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEKALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(11065..11583)
                     /locus_tag="VQ620_000542"
     CDS             complement(11065..11583)
                     /locus_tag="VQ620_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2763222.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(11573..12016)
                     /gene="tsaE"
                     /locus_tag="VQ620_000543"
     CDS             complement(11573..12016)
                     /gene="tsaE"
                     /locus_tag="VQ620_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET2763223.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(12102..12722)
                     /locus_tag="VQ620_000544"
     CDS             complement(12102..12722)
                     /locus_tag="VQ620_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763224.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(13105..13968)
                     /locus_tag="VQ620_000545"
                     /pseudo
     CDS             complement(13105..13968)
                     /locus_tag="VQ620_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            14133..14327
                     /locus_tag="VQ620_000546"
     CDS             14133..14327
                     /locus_tag="VQ620_000546"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763225.1"
                     /translation="MKKKRVSRMKKNRGIQKLAILVLLGVFMFSNTIPYQQFIQKNRQ
                     LEIRVQSQKKSNGLDVGKAD"
     gene            14642..14938
                     /locus_tag="VQ620_000547"
     CDS             14642..14938
                     /locus_tag="VQ620_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2763226.1"
                     /translation="MLKFRKVVASSLAVLAFSLALPVLAVSHRGGEWTYGGHHDPNNW
                     GAFSNYYHGSQYHWAYVGSNGRNNQKTVYAGARSAAYAFVNTNFGEQVTFDAGW"
     gene            15041..17056
                     /locus_tag="VQ620_000548"
     CDS             15041..17056
                     /locus_tag="VQ620_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2763227.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSYYYPNVVVHDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFIALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAGLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            17058..17690
                     /locus_tag="VQ620_000549"
     CDS             17058..17690
                     /locus_tag="VQ620_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2763228.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLTYLDLDKRIFELSGGESQRVALAKVILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDRLK"
     gene            17831..18109
                     /locus_tag="VQ620_000550"
     CDS             17831..18109
                     /locus_tag="VQ620_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HET2763229.1"
                     /translation="MKKFDNYIVEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(18618..19031)
                     /gene="ndk"
                     /locus_tag="VQ620_000551"
     CDS             complement(18618..19031)
                     /gene="ndk"
                     /locus_tag="VQ620_000551"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET2763230.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(19142..22819)
                     /gene="rpoC"
                     /locus_tag="VQ620_000552"
     CDS             complement(19142..22819)
                     /gene="rpoC"
                     /locus_tag="VQ620_000552"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET2763231.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKTEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPYAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(22852..26463)
                     /gene="rpoB"
                     /locus_tag="VQ620_000553"
     CDS             complement(22852..26463)
                     /gene="rpoB"
                     /locus_tag="VQ620_000553"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET2763232.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLKEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            26879..26949
                     /locus_tag="VQ620_000554"
     tRNA            26879..26949
                     /locus_tag="VQ620_000554"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26911..26913,aa:Cys,seq:gca)
     gene            complement(27147..28478)
                     /locus_tag="VQ620_000555"
     CDS             complement(27147..28478)
                     /locus_tag="VQ620_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HET2763233.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(28769..29593)
                     /gene="endA"
                     /locus_tag="VQ620_000556"
     CDS             complement(28769..29593)
                     /gene="endA"
                     /locus_tag="VQ620_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HET2763234.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQ
                     APEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(29632..29820)
                     /locus_tag="VQ620_000557"
     CDS             complement(29632..29820)
                     /locus_tag="VQ620_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET2763235.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDSW
                     AILSPAKWQELIHKFTGN"
     gene            complement(29813..31096)
                     /gene="murA"
                     /locus_tag="VQ620_000558"
     CDS             complement(29813..31096)
                     /gene="murA"
                     /locus_tag="VQ620_000558"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET2763236.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAECLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(31120..31350)
                     /locus_tag="VQ620_000559"
     CDS             complement(31120..31350)
                     /locus_tag="VQ620_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HET2763237.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTAENTRKVRV
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(31408..32445)
                     /locus_tag="VQ620_000560"
     CDS             complement(31408..32445)
                     /locus_tag="VQ620_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET2763238.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPNNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(32429..32917)
                     /gene="coaD"
                     /locus_tag="VQ620_000561"
     CDS             complement(32429..32917)
                     /gene="coaD"
                     /locus_tag="VQ620_000561"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET2763239.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLCVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(32907..33446)
                     /gene="rsmD"
                     /locus_tag="VQ620_000562"
     CDS             complement(32907..33446)
                     /gene="rsmD"
                     /locus_tag="VQ620_000562"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET2763240.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKTVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(33511..34503)
                     /locus_tag="VQ620_000563"
     CDS             complement(33511..34503)
                     /locus_tag="VQ620_000563"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HET2763241.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(34614..35297)
                     /locus_tag="VQ620_000564"
     CDS             complement(34614..35297)
                     /locus_tag="VQ620_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET2763242.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(35353..36093)
                     /locus_tag="VQ620_000565"
     CDS             complement(35353..36093)
                     /locus_tag="VQ620_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET2763243.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            36127..36405
                     /locus_tag="VQ620_000566"
     CDS             36127..36405
                     /locus_tag="VQ620_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET2763244.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            36483..37409
                     /gene="yidC"
                     /locus_tag="VQ620_000567"
     CDS             36483..37409
                     /gene="yidC"
                     /locus_tag="VQ620_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET2763245.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(37484..38278)
                     /gene="pflA"
                     /locus_tag="VQ620_000568"
     CDS             complement(37484..38278)
                     /gene="pflA"
                     /locus_tag="VQ620_000568"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HET2763246.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(38404..39654)
                     /locus_tag="VQ620_000569"
     CDS             complement(38404..39654)
                     /locus_tag="VQ620_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET2763247.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(39721..40548)
                     /gene="purR"
                     /locus_tag="VQ620_000570"
     CDS             complement(39721..40548)
                     /gene="purR"
                     /locus_tag="VQ620_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HET2763248.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLHEFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(40815..41756)
                     /locus_tag="VQ620_000571"
     CDS             complement(40815..41756)
                     /locus_tag="VQ620_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HET2763249.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(41746..43002)
                     /locus_tag="VQ620_000572"
     CDS             complement(41746..43002)
                     /locus_tag="VQ620_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET2763250.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(43004..43666)
                     /locus_tag="VQ620_000573"
     CDS             complement(43004..43666)
                     /locus_tag="VQ620_000573"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HET2763251.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDGFVGVDRGSLWILE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFKQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLASYMHQIEIEDGQNLITYCPEGISQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(43629..44285)
                     /locus_tag="VQ620_000574"
     CDS             complement(43629..44285)
                     /locus_tag="VQ620_000574"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET2763252.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDGHFVP
                     QISFGAGVVEALRPHSKMVFDCHLMVSNPEHHLEDFVRAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALRDEKGLNFEIEVDGGIDDQTIAQAKETGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(44296..45174)
                     /gene="rsgA"
                     /locus_tag="VQ620_000575"
     CDS             complement(44296..45174)
                     /gene="rsgA"
                     /locus_tag="VQ620_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET2763253.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDGGELDFYQQTYGDIGYDFVTSKEELLPLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     TAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGTIAPFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(45767..46540)
                     /locus_tag="VQ620_000576"
     CDS             complement(45767..46540)
                     /locus_tag="VQ620_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HET2763254.1"
                     /translation="MRKKLVNSLMCAVVLSGFPIATYANEKNIGPNITVSNGQTRLSI
                     PEKNGVEVIQSEENRVVYEVKEAAKIETSDGDMVAPIVVDSRITSINNQVVQGKTVYT
                     ADLEKATFVEEGYNKLAKPTNILDHIFGIQKVHAYTQNITTHQSQFDSTYSVELYTVV
                     HWVSYDGGVQKNITKISGGVDFHERDIEIESSSVEVRQGGWYSSQQKWFHPGARSSWE
                     YSTGFSKVGPGENRVVTNYTVNLIRYGDRWSAYLQNNPW"
     gene            complement(47230..48102)
                     /gene="rsmA"
                     /locus_tag="VQ620_000577"
     CDS             complement(47230..48102)
                     /gene="rsmA"
                     /locus_tag="VQ620_000577"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET2763255.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAEVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPVPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLE
                     EFASLADALKGQGL"
BASE COUNT        14216 a        10657 c         8912 g        14500 t
ORIGIN      
        1 tggtttcgca ggaagtctat tatatatgca cagacatatg aaatcttttt gagacagcag
       61 ttatgatata tttatccatt cacaacatct tctgtactaa tactacaata ttggtacatt
      121 ttgattacac aatcgtttca aaaaatatga tggaagcctg agtattttat agtaattccc
      181 ttcctatcaa ctagatcaac cagttgagtg ttgaatgttg aaacacccta gcctcagcaa
      241 aggactatga gcagcatttc ctagccaata ggacgctcca aatcacctat tctaaatcca
      301 tcactagctt ggtcagtaag atacagactg caaggcgaga ctgacgtgtt tgatgagatt
      361 ttggaagagt attacaatca tagattcacc agtagacttg accgcagatt taactatagt
      421 agattgaaac tagaatagta catctctgct tctaaaacat tgttagaaat cgatttgact
      481 gtcctgatca atttgtcctg ttctcgtttc atttcactat actaaaaaac cacctgaaac
      541 aaatcaagtg actagaggat aaaaacaaag actaggcaaa aagttttgaa aagcaaaaaa
      601 cgcataatat caggtcttga aaaaccttga tactatacgt ttgattgtgg gaagattgac
      661 ttcattttct cctgaaattg aagttttttc tagtttccag atatagacga attcaatata
      721 gccatctcgc tacaattatt ttaaccatcg agaaaatttt tcagaatttc aaaaatttca
      781 ttttgatcca atgaaatgtc atcattttga agaatagttg acttcacagg ctctaattct
      841 atcacaaagc ggtgtatgtc tgcttttacc gcactacttg atgttatagc cgataacttt
      901 tcatctccta gcaaggaagc tgtcaaacgg gtaatgtcat tcaaatgctt tttaatggac
      961 ttacttaaac cttgagcacc tgtagcagag cgttccctca tatccagcca agctttagcc
     1021 ttaaaaataa ttaatactca atgaaaatca aagagcaaac taggaagcta gctgcaggct
     1081 gctcaaaaca ctgttttgag gttgtagatg gaagctgacg tggtttgaag agattttcga
     1141 agagtataaa ccacaattac tcaataccga atacccctga atggtttctt tttcatgcac
     1201 caatatatta taataatctt catccaataa taaggctgat aaactagcat cttggtcaaa
     1261 atgtaaggga atttctcgac cgtccttctt taatggatat tctggtaaga tactaaatag
     1321 ttcaatcata gaaggaaact caggattagt tgttgtaaat cgaaaaagct gcgctttctc
     1381 atctttctga cttccttgat actctcccaa ttctaaaaaa tgattcaagg tagtataaaa
     1441 ttccttattt tttacttcat aaatgatgac catatcataa tcttttgttg tgcgactttt
     1501 aaatccttgc gaatccaata cgatagaggt agcagttccc ccaatcagaa cataatagtt
     1561 ctgaaaatcc gcaaacattt cttgaaaaat aactttcgta ttagctggca tcatcttctc
     1621 ccagatactg taatatcata ttttctagtg cttcactctc ttcctctata cgtgggtcat
     1681 catcatcttt taaggtcaaa taaagagaaa tcggatctac aaattgttta tcatgattat
     1741 ttttaaaatc attccaaaac tcagatacaa aaggacgata tttccatatc tctagcatct
     1801 ttcattttaa aacatgctga gaaagtggca aggataactg attgaatttt ctctgccata
     1861 tgacatagct agtattttca tccgtttcag ctaaaaaagt tgaatgcgac aaagcataag
     1921 caccaccata tagaaggtta gaaacagatt ttatctgctt tatatcgcca tctggcaata
     1981 aaaaggcaat ccacgttaat tgttcgctag gtgttaattc cttagggatt tcagtatcat
     2041 tcgcattgag tactagtccc aatggaggga agaagaggtt tcccttaaag tctacaaacg
     2101 gaactctagc ttgaagtaat tgcttttttt cactgtctga taacttcgaa aacaccaaaa
     2161 caacatccat attggctttt tcacccatag tgcgagcctg agtaacaaat gaactcaaac
     2221 tcccccttct cttttcttta atcaataaaa atgattcctt atgaaaagtc atttgagtat
     2281 actcaaacct ttttacaaaa ctgattggta aagtcaaagt cagttcctca atactgacat
     2341 caacataaaa ggattgaaaa attgacatta tctttttgac attcatgttt tttccttatt
     2401 ctatcatttt tattatattt tatcattcgg tataataaaa tataagtttt ttgataaaat
     2461 aaccaaaaac tactcgctat cccctctcac attaccctac atatcgtttg acatgcgact
     2521 gatagttcag gaaaacttca aggagaactt ttctctcatc cactatgcag gacttactat
     2581 ttcacttcta ctcctatagg ctcaatttga cacttttctt tttcgaattg ctcatatgcc
     2641 tcctgaatag tgagttcgat ttatccaaat ttatttttgt ctattttgat gaatacttcc
     2701 acgaagatag tctgaaaatg aatttgcacc aattttcctt ctctttttaa gcatattatt
     2761 ccagatatga ttgccctctt gttcagtaat aaattacgct ttaaacgctt caatcagtat
     2821 atctcctgtt gtcatatgtt ctaaagaaaa ttcttctaca tatggtttaa catctcttag
     2881 gttattactt cctaatatcc cattatgctt tttcgctaag aaaatagaag tcgcttctcc
     2941 cttaccaata atcttgttac tatcataatt tcttgttaaa tctctatata atgcgtattc
     3001 ttcagttcca atgtctatgc tcacaatctc agctgaaccc ttagttacca actggtctat
     3061 cctagatttt aaatggggaa ttgtaggtat attgatttca tcatacacct cttgtggaat
     3121 aacaatttta cccgaataga gcttttctaa aagatgttca gtgccaaccc ataaaaatgc
     3181 tgaaatacaa tctgcatcaa taaatacacg actagtcaaa cgacaactcc ccctcttcat
     3241 ctagcccata tacaatatca tatctgaaag catctagtaa cagttcctca tacttccctt
     3301 gcgaaattct gttattttct aaaagttgtt cagtcgattt aatatagtgc cctaatacca
     3361 tttccttttt actttctgac aaaggacgat ataaacttgt atcatagcct aatcttgaag
     3421 ctgtctctat aacactaata tccatatttt taatttcttc tgcatcaagg tatccatcat
     3481 ttctcaatct atataacata gctttatgac tgataccata aaactgaccc aattttataa
     3541 tatcttctac ttcaagatga gttctattgg cattttctct gatttcctca accatcctat
     3601 acagtgaaga tgggaaaatt aaaaaataag aagcaaactg atccgctttt ctttcagttt
     3661 catctccttc accaatcaag ataagactga ctgaactctt cttcacctca tcataataaa
     3721 gatgatacag tccatgtgct aaagaaaatc tttaccttcc taatggcatg tctgaattga
     3781 ctgcaatgag actgaactga gttcctttat aacagactcc gctaatattc tttccgagtc
     3841 cataaaatac cagcgtcaaa ttttctatct tttgtaccaa tttaaaaata tctatcggcg
     3901 attcaccatc agctcccaac ttttttctaa gatttgaagc tttattactt aaatccattc
     3961 gtgatattcc tttcaccttt ttccattttg gcaatcatgc tttgattcaa agacaaatac
     4021 tcagcaatct tatcttgagt aagatttgaa gatattctaa gttcttttat tctttttcca
     4081 acatcacata catttatcat attcatacac ctctcataaa aagaatagca cattcttgtc
     4141 ataatttttt aaataaaaaa attatgacaa aacaaggaag caatttattg atgctactta
     4201 aaaatctaaa attgatgata ttaaacctat ttgatgagtt cctatcaaaa atcgtatctt
     4261 caacctcaaa acagtactta aagctatccg actcggttta cattgtcaaa tttagatttt
     4321 atttgagcat aactttctag tttgcttttt gatttttgtt taatatagta acaaaaattg
     4381 aaggaaatat ctccacaaga aaacgcatac tattaagctt tttcaagacc taataatatg
     4441 cgctgttctg atttgaaaga cattccatta ttattttact gtaatcaagc cttcaggctc
     4501 tactgtgaag tctggcttgt ctgccagtgt tccgtctggt ttgaggtagt accagcctgt
     4561 tccgtccgct gactggataa aggcatttga taccatggcg ccttctttag cgtctaagta
     4621 gtaccaagtg tccttgtact tgacccagcc tgtcttcatg gcaccttctt cgttgaaata
     4681 gtaccactta tcagcgattt tcttccagcc tgtagccatt tcgcctgagt tgtcgaacca
     4741 gtaccagttg ccgtctgtgt gcttcctcca gcggtctgca agcatatagc ctgaactgtc
     4801 aaagtagtac caagtgccat tgattttctc aaacttgtct tttggataag agccgtctga
     4861 atgtacgtac cagtagccag tgtcattctt ctgccagcct gtttcaatcg tcaagccgtt
     4921 ctcaatatca tgcttaaact gctcacggct aatgccccat ttagcaagat atggataagg
     4981 gtcaacgtgg tctgagtggt tgtttggttg gttattcgtg caatactcgt gcgttttaat
     5041 tccagctaaa ctccctgtat caagcgtttt cggcaaacct gcttcatctg ctagattgcg
     5101 taagagttcg atataaaggc ggtagtccgt catgaactct tctttggttg aatggctttc
     5161 aatcagttca accgctgcat aggtctcagc attccaaccg cccccaacgt cccaggcacc
     5221 attatcaaca ggtcctacct gcatgatgca accgttccca acaatgtgcg agaaaaaacc
     5281 taattctggg tctttccgcc agtgataatc cgcttcattc tgtacggttg aatgcggatt
     5341 cccagttgag tgtgcgtgta cttgcctata tggttgcacg ccgacttgag gcaaatctgt
     5401 tcttaattta ctcacattaa tttccatatt ctactcctta tcaattaaaa caactcattt
     5461 tttacaatcc agaaccagaa actcctttat ttctacctta caaagaagac aatcttagtc
     5521 acgattaagc ttgtagatag aacctcaaaa cgcactattt tgacactgta aataggactg
     5581 acaaggtctg cattctatct acaataacac cccagactaa aaagcttttc aaaagtatat
     5641 ttttacagtc tctatatgtc cttttcataa atactatact ttattatatc acataaaaga
     5701 agtcaaaagt ctgttaaact attttcaaca ccaaactaaa gaagagaaca caagtttttt
     5761 cgatgttcac tagaggaaat ggattttatt cagtaaatcc aactaggatt gcactttggt
     5821 tgccaaaatt gcctttcctt cttttatcaa gggatgacgg aatagtgaga agtacagttg
     5881 aactgtcatg gcaacccaga taagaggttc acaaaggata acacccttat atcctgccca
     5941 aggaataatc aaaaccacaa aaacgatttt tccgattagt tcaataaagc tagaaactag
     6001 aggaaggatc ttctgcccca agccctgcaa gcaattgcga taaatcaaca agaggctcaa
     6061 aatgggataa aaggctgaac tgatttgcag atagagactt ccattttcta tcaagtaacc
     6121 atctgtcgaa ctagccaaga aggaaaccaa ggctggactg gcaaaaaaga ggaaaataca
     6181 aacaaaaact gcccaggata tacttaaacg actgccgatt cgaagacctt gaacaatgcg
     6241 gtcaggtcgc ttagctccta gattctgaga agcaaaggtc gtcattgatg cagaaatagc
     6301 ggtcatagga agaagggcaa aggtcataat gcgtcgagct gccgtctggg cactaataat
     6361 cactgcacca aatgtattaa cagaaaactg taaaatcaca ctgccgatag atacaattga
     6421 actcatcaag cccatagcca aaccttgctc caagagatcc gcgtacaagc ttttgtccca
     6481 tttgaaatgt ttaaactgtg gcaagagttc tggcacactt ttacgaatat aataaaagca
     6541 gagaaccgct gataaacctt gcgaaataat ggtagcaagt cctgcggatt gaactcccag
     6601 atgcaattgc gtaataaaat agagatccag aaccacatta accaaggcag agaaaatcag
     6661 aaatcccagg gctgctagac tgtcaccaat agaccgcaac aagcctgcaa aaagattata
     6721 agcaaagctg acacctacac aggtcacaat catagaaata tattgataag attgaggaag
     6781 aatttctgca ggagtatcta agtattgcaa gagatgatac aagccaagaa agcccagcaa
     6841 cataactaga atgctcaaaa gagcacctaa aatccaagta gctgctactg cttccttgat
     6901 tttagtgaaa ttccgagccc cataataacg agcaatgaca atccccatgc cattgccaac
     6961 accaagtgta aaacctacaa tcaggtcaaa aatcgctgtc gtcgctccta ctgcagccaa
     7021 ggattcttga ccaagaaatc gtccaacaat caagacatca gcagtgttat agagctgttg
     7081 aaaaatattt gatagcaaaa ttggaaaggt gaagcttaag agcgagggaa gaatcggacc
     7141 atgtatcagg tccactgttc gttttttatt cataaagcta ttatatcagc tttccagagg
     7201 agcgacaaga aacagcaaac ttatatatag cggaatgaaa taaggaaagt caaatcgatt
     7261 tttaacaatg ttttagaagt agatatgtac tattctagtt tcgatctatt atagttggtt
     7321 gagtttggaa tagtacgctg tagctgctaa aacatttcta gaaattaatt tgactttcct
     7381 aatagagttg ttcatatctt atttcaagct actatatata atctaaaaac ttgaatcaaa
     7441 aatcgaaaaa gtagcggatt tctccactgc tttaacagct tattcttcaa tttcgatttc
     7501 aagttcatcg cctaagtcaa gcggaacttc ttcattcact gcttcttctt tctttggctc
     7561 atcttttttg ttttcagtat cttgttctga aacttcttct ccatcaatca agccaaattt
     7621 agaacggact tgcttatcaa tttcatcaaa gatttctggg tgctctgcca agtatttctt
     7681 agcattctca gaaccttgcc caattttttc atctttgtaa gaataccaag cccctgcttt
     7741 tttgataata tccaaatcgc ttgcaatctt caaaagctca ccagtcttag aaattccttc
     7801 tccgtacata atttcaacta cggcttcctt aaacggtgga gctaccttat tttttacaac
     7861 cttaatctta gtttctttac cgacattggt ttctttttgg tcaccagttc ccttaatttg
     7921 tgtattacca cgaacatcca agcggactga agcatagaat ttcaaagcac gtccgcccgg
     7981 tgttgtttct ggatttccaa acatcactcc aactttttca cgcaattggt tgataaaaat
     8041 ggcaattgtt ttggttttat tgatagaggc gccaagttta cgcatggcct ggctcatcat
     8101 acgagcctgc aaaccaacat ggctatctcc gatatctcca tcaatttccg cacgaggaac
     8161 aagggcagca actgagtcga ctacgacaag atcaactgca cctgagtcaa tcaattttcc
     8221 cgcaatctca agaccttgct ctcctgagtc tggttgagac aagagcaatt cgtcaatatt
     8281 gacaccaagg gccgcagcat aagctggatc aagggcatgt tccgcatcga taaaagcagc
     8341 aatcccacct tctttttgcg cttgtgcaac tgcatgaagg gcaaccgttg tcttaccaga
     8401 tgactctggg ccatagattt cgatgatacg tcccttagga taaccacctg agccaagggc
     8461 aatgtcaaga gccaaagaac ctgagctcat cacttgcacc ttttgctccg cacgttcacc
     8521 caaacgcatg attgatcctt taccaaagtc tttctcaatc aatttaagag cgtcattcaa
     8581 ggccttttca cgttctgccc caaatttttt tgaaatttct tctaattttt ttggtttttt
     8641 cgccattcta ttctcctaca ttctaatgat cctcagacct atctactatt atatcaaaag
     8701 ttagtcactt aataaagcct tgcgaactag gttaaaggca tgcataaccg caatgtgacg
     8761 tacatctgct cggcttctgc ctccaatatt aaccttgata acctcagttc cttgatcttg
     8821 cgccaagcct atgaagactg tcccaactgg gtgtccttct aggctatctg gtcctgccac
     8881 tccagtcaaa ctaattccaa aatcagactg ggtcttgctt cgtgcctgct cagccatctt
     8941 ctgagctgta aattcagaca ccacaccatg ttcttccaaa ttcttggcag gaatatccaa
     9001 catccttgat ttttcctcca agctataggt cacaaaacca cccttaaata tacttgaaat
     9061 tccagaaaaa ttcgccacgg tagcttggaa aagacctgcc gtcaaactct ctgcagccgc
     9121 gatggttttc ccttgccttt tcagttcttc taccacaatg ctggctaaac tagtttcttc
     9181 cccataacca tagcaaaagt ctcgtaaaga aattccttcg aaagtctggc agtccaagat
     9241 ttgattttcc aagatatcca gcgcttgatt cgcctcttct tgactgctag cctttgttga
     9301 cagacgtaga gtgacttctc ctgtcttggc ataaggggcc aaggtaggat cgacctgatt
     9361 atcaattaaa tcagccaaaa tcgtaaccag ctggctctcg ccaatcccaa agaaacgaag
     9421 aactcgggaa tacagcttgc tccctgtcat caacttgggt agaagtaggt ttaagaccat
     9481 gggtttcaat tcacttggcg gacctggaag gacgacatag gtcactccgt ctacttctaa
     9541 ttttcctcca acagccagtc ctgtttcgtt tggcagtgga atcgctcctt ctacaatttg
     9601 agcttgtctt tcgttattcg gtgttcgggc atagtctggt cgcagggcaa aaaagatatc
     9661 caacttctcc tgagcctgag gatcaaagac taatgctttc cctaaaaatt tagctagggt
     9721 ttgtttggtt aggtcgtcct cagttggccc caaaccgcct gtcaaaatca ccagactgct
     9781 acgttgactg gcaatctcaa gcaaagacaa gagacgaact tcattgtctc ctacagccgt
     9841 ctgaaaatat acatctaccc caatctcagc tagtttttcc gacaaaaact gggcattggt
     9901 gttgacaatc tgtcctgtca aaatctctgt tccaacagca atgatttctg ctttcatgtt
     9961 tcctcctacc tatctattcg tatttttttg aaaaaatcgc aggaattttc ctacgattga
    10021 ttttttattt gtatcaaaag ttaattatct tcatcaccaa caggtgctct gccaaataaa
    10081 tcttcaaata aaaccgcatt ggtttcaagc tgagtaactt cttcttgtcc caaagaacgt
    10141 cggagtatat tttgcatttc caacatatgt gctcttgaaa caatctggta agaaacacct
    10201 tgaagtatct ctccttcacc ctgcaactgc tgagtttcaa tggttttaaa tgaatcttta
    10261 tagcctagca agttagggat actttttgca gacaaatcaa tattggtctg catattgtca
    10321 ctcaaagctt ttagaatctc ttgataatga ccaacgctat ttaaactgag agctttttcc
    10381 atgacttttt gaataacttc acgttgacgt ttttgacgac cataatcccc ctcaggatct
    10441 tggtaacgca ttcgtgcata gactagggct ttttctcccc caatatgttg ctccccaaca
    10501 ccgatagaaa tagtattaaa ttcttcttgg tcactgatag aaattgggaa acctaggata
    10561 ttattgactg taatacctcc tactgcatcc actagttttt gcaaccctct catattgacc
    10621 atcacatagc gatcaatatg gatattcatc attttttgaa tggtttctat agcaagctct
    10681 gctccaccat ctgcatatgc tgagttcagt ttcgcttcat gagcctgacc attccctgat
    10741 tcaatgcgcg tcagaatatc ccgctctaaa ctcatcattg ttgttttttt cgttttagga
    10801 ttcactgtca tcaagatcat gctatcactt ctaccgaccc aagtttcagt tcgttcaaca
    10861 tttccggtgt ccactcccat taacagaatg gttagaggtt cagtcgcttc aataaccttg
    10921 gtttcttcgc cgattttttt ataggtttta gctaaggttt ctgtcccttg ttgataaata
    10981 gtataagcaa aaacacctac tcctactaca gttacagaaa gtaaagctag caccattcca
    11041 ataatttttt taaccatatt tctactaacc tatcagttta cccatcaagt aaacatcgat
    11101 aaatttccct tcttctatat atgccccacg ctcttggcta ccttcaatga caaagccatg
    11161 cttttgataa agatggactg ctgcttgatt acgagtttgg acagtcagtt ggagacgacg
    11221 cagaatgcca cttgcttgtg cccactctat cgcttcttct agcaacaaac ttcccaagcc
    11281 attattccaa tatctttttc caatcacaat gaagagatct ccaatatgac ggactctctt
    11341 acgctgatca gctgtaatat ttacaatacc agcaattttg ccatttaaga atgcaagtaa
    11401 ggttatctga ttgtccgaac tagcttgctt gttgaggaat atttccatct cctcactagt
    11461 caagagaata ccatctccgt ctaggctggt aaagtctgtc tccaaactca cacgatttaa
    11521 aaaggccact aattcagctg catctttggg ctctgcttcc ctaatgagca attcatactc
    11581 catattgaag ctcctctaac aatttctcag cacgcaaacc ctttgcctga aaatttaaac
    11641 ggcgtccatc tgcttctttt agaatttcca attctaaata agcatctggc aaggcatctc
    11701 ctaagagatt tccccactca ataacagtca cgccgccacc aaagataaac tcatccaagt
    11761 cgatagaatc agcatctcct tcaatacgat aaacatctag gtgataaagt ggaagtcgac
    11821 cttcatactc tctcacgata gtataggtgg gacttttaat catttgagaa atctgtaatc
    11881 cttttgcaag tcctttagta aaggtcgttt tacctgcacc cagttctcca gttaagatta
    11941 aaacatcatt ctttgctaat agatggccca aacgctcccc taaggcttgc aactcttctt
    12001 cattttttgt gtacatactc ttattatacc aaaaactttt cttttgtgtc tattttccta
    12061 ctaaacttat catcataaca tccataaaaa acaggctttc tctaaaagaa aatgagcgta
    12121 acaatgacca atacaagatc tcggaaaata tgaccataaa aggaaacttc cttcttaacc
    12181 gaatttggga caagataggc tgcaaaaaac aagcccagtc caatataaat cagaagtgag
    12241 acaatggtca ttggatttct taagaaaaga agtgttgcta aaatagtcac caacactgtc
    12301 ttttttctgt ctagcatagc aagaaaatcg cgcacgtatt ttttcaaggg taaaaaaatc
    12361 agcaaatcta gcccaaatag gaaaaagaag gatggcaata aaaagtcaac taattcttgc
    12421 tgcagcgtat ttttgatgaa caagttatct gacaaaacaa gaacagctcc taacaaatta
    12481 attaagagta acatactgta aaaaagcttc accgacttct tactggctag gacactatgg
    12541 acttcttgct tacgggtata aagataattt actccagcac agattcctga aacgaaaacc
    12601 atgctcccga tgaaaaaagc tgtactttgt ttaaaggaca agatgcattc cttccatagg
    12661 aaacagctac tcaaactgat ttgaattaaa gctaacaaaa ataagattct cattgatttc
    12721 atcttctctc tcccttccta ccaatcatta tactaggaga aaagagagaa ctgtttctaa
    12781 tcttctcaaa tgtctcttta agacgctaaa caaacactag agactaatac tcaatgaaaa
    12841 tcaaagagca aactaggtag ctagccacag gttgctcaaa acactgtttt gagattgcag
    12901 atagagctga cgtgatttga agagattttc gaagaatata aatttgaaat catgaaaatc
    12961 cgtcaaacgg gtggttgttt tgtctcgcac ctcacggagc gagacggact cagagtcaca
    13021 taattataag gctgatagta ttaatctaac tatcagcctt acaggttatt taacgtttca
    13081 gaaaaactat aatgtcaaga ttaactaaac agtatctaat tccttcaaat aattttctat
    13141 cttcatcagc attaaaggat tgttataaat cttgcatagc tctcttgctt ctatataata
    13201 atttttgact tgttctctgt ctagaaattt ggctccagca tttcctacaa gaataagtag
    13261 aggagccaat tggtagcttg tctgtctttg tttacagagt tcgatagtct caagagcttc
    13321 ttggatggct tcattatatt tttcctttga tactaggtag tgagcgtaat tgtaacgaac
    13381 tctgatgtag ccaaataaaa actcttgatg ctcaaaattt tttgtctgat ataactccat
    13441 caaatgagag tagtttacct catattcttg ttcacgaccc actaaggaat agaaattaga
    13501 tagagtattc aacgccttta aataaatcaa agtatttgag gagactttta ataatatatt
    13561 ttccaatgaa gaaattgctt cacacttact gtcatattga tagaagtcaa taatagcttt
    13621 aatccattca aggtaagttc ggtcttctag tgttagaaaa gtacttcgtt caatctctat
    13681 tctataaata tattccaaat tttcataatt tctatcatct aataggcgag cagataattg
    13741 cttgaaatta gagaggttag atttaatttc aatttgttca ttaaaaaaat aatctaatgg
    13801 cacttcgagt cgttgtgaga gtttgaacaa aaggtctgcg gagggaatga aataccctct
    13861 ctcaatttta ctaatctggc tctattcaca aattccttct gcaagagttt gttgggagag
    13921 tctcaactct tttcttttca atctgaattt ctctgcgagt gtattcatta aagataataa
    13981 accttcctat ttgcttaatt tcattataaa atttttagtt caaaatagca agttaaagga
    14041 ataaataatt ctttgcggta ataaacaaaa aaaagtcaaa aattacataa aaatgcttgg
    14101 ctaaataaaa tatatttgtt aaaataagga taatgaaaaa gaaaagggtt tcacgaatga
    14161 agaaaaatcg tggaattcaa aaattagcta tattagtatt actaggtgtt tttatgttta
    14221 gtaacacaat tccttaccaa cagtttattc agaagaatag acaattggag attcgagtcc
    14281 aatcgcaaaa gaagtccaat ggtcttgatg ttgggaaggc tgattgattt tatattactt
    14341 taaaattcaa ctatttagag aaaggtattc tgatgagagc ttgtaacaat accagataga
    14401 tataagaatg gagatgtttg ataaaatgct ttcatagcta accaactgtt gaaaattatg
    14461 agttacaaaa tctaaagtat ttaagattgg tttataaaat taggtctctg cctgtagaaa
    14521 ggaacgtgca gttagaaagg aggaaaaaat aggatactat aattcagact gagatttcta
    14581 attaaaatat atagttgcat ttcgctcatt ttcataatat ttcattcaga ggagtaataa
    14641 tatgttaaaa tttagaaaag tagttgcatc atcccttgcc gttttggcat ttagtttggc
    14701 tcttcctgtt ttggcagttt ctcaccgtgg tggcgagtgg acctatggag gccatcatga
    14761 tccaaataat tggggagcat tttcgaatta ttatcatggt tcacagtatc actgggccta
    14821 tgttggaagt aatggacgta acaatcaaaa aacagtttat gcaggagcac gtagtgcggc
    14881 ctatgcgttt gttaatacaa attttgggga gcaagttaca tttgatgcgg gatggtaaag
    14941 aataattgaa gaagattctt ttatcaatag ttcagtaagc ttgttagttt atagagggca
    15001 ggggtatttc tgccctctgt tttagaataa ggaatgcact atgaaaaaat tatttatact
    15061 catatccaac ctgcttgcta gtttgttctt tgtttgggta tttaccattt ggactgatac
    15121 atatgtctcc tattattacc ccaacgttgt tgtacatgat tcttcccctg aaacaacttt
    15181 tcagcatgtt gctacacgct tggaaaaact agcagaagaa acagatagct ttattgcgat
    15241 tcaacaccaa gatcctaact cagaaggtac tacagttttt tcttatacga cctttgggga
    15301 cggaaagttg cctgatgggc ttcaggaaaa aaaactagaa gatgctcaaa gtagtagtgt
    15361 tgaaacaaac tattttgtat tcgatggaca cctagatatt cacttgctaa gggaggagct
    15421 aagtcaactt ggcttgacta acatgaacct gacaattcca tctaaactat ctacgttaat
    15481 ggctatcttt agtaacggat ttcagttaat cagtctattg attttcatct taacctttat
    15541 agcactgact ttaattagtc aaattagcca attacggtcg tcgggcattc gcctcatttc
    15601 aggagagaaa cggtggtcca ttttccttag accagttggt gaggatttga aagggatagc
    15661 tgttggtttc agtttagctg gcgtgctcgc cattcttatg caaaaaattc tgtcgcttcc
    15721 aacgcaatcc ctaatgacca taggagcagg cttgctcagt tataatctca tcttgttgtc
    15781 gatctccctg tttttcgctc aactcttcgc agttgggata aaaaagatac acctgatgca
    15841 gattataaaa gggcaagtgc ctgtcagagg aatcattagt ttgattttaa tcggtcaact
    15901 attagcgatt attattgtaa cgctgggaat agggagtagc ttaaagtatt cccaagcctg
    15961 gcaacagcat cggattggac aagaggcttg gagtcaggaa aggcaactga ttaccctatc
    16021 aatcagccgt gaaggaacga gtcctggttt tgatgaacaa gctcaaagga aactcagaac
    16081 ttggtatcaa ttgatggatc tggctgtttc agaacaaaag gctttcttat ctagacacca
    16141 gttaattgac cgtactttgc aaaatggcat ggcttcctcc aaaaacttga taacctctac
    16201 agaatggcac gattacaacc cgaatggcaa tgtccttatc gtcacaccgc aatacttgga
    16261 gcgtcaaaac attcctgtag atacaactat tgagcaaaag atgaatcacc ttgatgtagg
    16321 ggagtttgtc ttattgctgc ctgaacacct ccgttcagag gaagaacatt ataaatctgt
    16381 ttttgaagac gacttaacca gtcgcatgtc tagtcaagat gaacgacagc aaatgactgc
    16441 tacggtaggt tatttagaat caggtcagga tcgttttgtg tataatacga cccctatttc
    16501 ttaccagcag tttttgaaag atccaatcat cattgttata acaccccaat caactggtcc
    16561 acagtccatt ttgttttgga tagacgcagt acagaactac gttctcttta atcaattgtc
    16621 tgatgcccag gagcttatcc agagacaagg cattgaaaat tgggtctcag aaatgcaaac
    16681 aggttaccac aactacatca cattattgga taatattcag agggaacgtt gggtaatgct
    16741 agcaggagct gggcttggga ttgcaacttc aatcttgttg tttaacacta tgaataggct
    16801 ctactttgaa gaatttagac gtgccatttt tatcaaacgc attgcaggtc tcaggttctt
    16861 agaaatccat cgcacttatc tctttgctca actgggtgtg tttttactgg gatttgttgc
    16921 gagtgtattt cttcaggtag agataggagt tgctttctta gtcttgttac tctttactgg
    16981 tctatctctt ttacagttac atgtccaaat gcagaaagaa aacaagatgt ccatgcttgt
    17041 tttgaaggga ggttaatatg attgaactta aacaggtgag taaatctttt ggagaacgag
    17101 agttattttc gaatctttca atgacatttg aggctggaaa agtctatgcc ttaattggtt
    17161 caagtggtag cggaaaaaca accttgatga acatgattgg gaaattagaa ccttatgatg
    17221 ggacgatttt ttaccgaggt aaagacttgg ccaattataa atcgagtgat tttttccgtc
    17281 acgaattggg ctacctcttc cagaactttg gcttaattga aaaccaaagt attgaagaaa
    17341 accttaagct aggtctcatt ggtcaaaagt tgagtcggtc ggaacagcgg ttgaggcaga
    17401 agcaggcttt agaacaggtc ggcctgactt atcttgacct agataagcgc atctttgagt
    17461 tatcgggcgg agaatcgcaa cgggttgcct tggcaaaagt tatcttaaag aatccaccct
    17521 ttattctggc agatgagcca acagcttcaa tagacccagc aacttctcag ttgattatgg
    17581 agattctgct atctcttcga gatgataata ggctaatcat tatcgcaaca cataatccag
    17641 caatttggga gatggctgat gaagtgttca cgatggatcg tctgaaataa aaatccttgt
    17701 ttttaattgc acgatgagtt actgaaatat tatcatgaat caataattgg agttaattta
    17761 gaattgtact ttattaatat tgaggtaact ttttcttgat aaaggaagaa atagtggaga
    17821 ggaagttaga atgaaaaaat tcgacaatta tattgttgag aagccttgcg attctaattc
    17881 agataaactg caaaaaatct taataattga aaatttggta gatgatattt tgcaatttcc
    17941 tctcagaatc aataatagtg taggagagat tttcctccta caaccgtttc aaaagaaaac
    18001 tatctttatt ccatgttatt ttgaggaaga tattgtgaaa gtcaaagatg atgataaagt
    18061 tgagtggaat ttgttagaat ttcaaaaatt tagagcattt ttggcttagt aatctgtgtt
    18121 gaaggctcaa aacctatggt aaaaaagtcg ctttgaaaac gtattgcctc caaagattta
    18181 gttaaataat gatttaacac aaaaagaaat tattgaagtt ctggaaagat gttgtttcag
    18241 tattgagaaa aggtgggaaa aacttgcgat tttcacagag aaaggaagaa aaagtataga
    18301 aatatagtca attgaaacaa gaacaggaca aaagaacctt ttgtgccata tttttctcct
    18361 ttcgctttac aattggcttg aacaccttta ttgtatcgcg tttggagttt ttttggtata
    18421 accttcgacg cgcacccgca tagcgggtgt ttttttgtct cgcacctaac ggagcgagac
    18481 gaactaatag tcacttaatc aaaaaacgca ccatatcaaa aactaaaaag tttgatatca
    18541 tgcgtcatgt cttaaactaa ttgactatac tttctattca aatgagcttt taaccaattg
    18601 attgagccaa tccactctta aaaccaaaga gcaatttctc gcttagctga ctcttctgaa
    18661 tctgaaccat gtacaacatt ttggataacc tcattttctc cagcagcttt tgcaaaatca
    18721 cctcgaatag tgcctggtaa agcttcttct ggacgagttg cacccatcat ggtccgccaa
    18781 gtttcgatta ctttgggacc agaaatgaca cccacaagaa ctggacctga agtcatgaat
    18841 tcacgaatcg gtgggtaaaa actctgacca accaagtcct gatagtgctg gtcaatcaac
    18901 tcttctgaaa cttgtgaacg aaactccaat ttttcgattg taaatccacg ttgttcgatg
    18961 cgctttaaca cttcacccac tagccctctt tttacaccat ctggtttgat gataaagaat
    19021 gtttgttcca tacccgtctc ctttgtcagc ttctttcttt tattttacca catttcgtgg
    19081 aaaaatggag aaagttttca gaatagagaa tgagagaacc cgagggttct ctcattctct
    19141 attattctac tgtttcttcc acagtttcaa cggcagtatc cacaactact tctgttgttt
    19201 cttcatttcc ttcttcctct actggaggat taaggtattc ttcttcgttg acagcatatg
    19261 gttcaaggtt acggtaacgg gccataccag taccagctgg gatgatctta ccgatgataa
    19321 cattttcttt aagtccaagg agatggtctt tcttaccacg gatagctgcg tcagtaagga
    19381 cacgagttgt ttcctggaag gaagccgctg acaagaaact gtttgtttca agtgaggctt
    19441 tggtaattcc cataaggact gggcgacctg tcgctggaac tccacctgcg ataaggacat
    19501 ctttgttggc atctgtaaag tcattgatat ccatgagggt acccatgaga agatctgtat
    19561 cacctggatc catgacacgg actttacgga tcatttgacg aaccattacc tcgatgtgtt
    19621 tgtcaccgat ttctacccct tggctacggt aaactttttg tacttcaccg agaaggtacg
    19681 tttcaactga caagacatca cgaactgcaa ggagacgttt tggttggata gaaccttctg
    19741 tcagagcagc accacgcgct acttggcccc caacttcgac acgcatacga gctgtaaatg
    19801 gaacgacata ttcaccttcg ccagtttcac ccttaacaaa gactttcttg gtacgagttg
    19861 atgcatcttc ttcgatagca gtaacttgtc ctttaacctc tgtgataacc gcttcccctt
    19921 taggattgcg ggcttcaaag atttcttgga cacgaggaag accctgagtg atatcggtat
    19981 ttgaggcaac cccacctgtg tggaaggtac gcattgtaag ctgtgtacca ggttccccga
    20041 tagattgggc agcaatggta ccaactgctt caccaacttc aaccgcatca ccagtcgcca
    20101 agttgatacc gtaacagtga cggcagacac cgtgacgagt gttacatgta aatacagaac
    20161 ggatagtcac ttcttccaca ccagcattga caatttcacg cgccttgtct tctgtaatca
    20221 attcatttgg accaataatc actgcaccag tttctggatg tttaacagtt ttcttagtgt
    20281 aacgaccgtt gagacgctct tcgagagact cgatcatctc ttttccttct gcgatagaac
    20341 ggatcaagag accacggtca gttccacagt cgtcctcacg gatgataacg tcttgggcaa
    20401 cgtcgaccaa acgacgagtc aagtaacctg agtcggctgt cttaagggcc gtatcggtca
    20461 tacctttacg agcaccgtga gttgagaaga acatttccaa taccgacaaa ccttcgcgga
    20521 agtttgaaag gattggcaat tccatgatac gtccattcgg agcagccatc agaccacgca
    20581 taccggcaag ctgtgagaag tttgagatgt taccacgggc tccagagtcc atcatcataa
    20641 cgattgggtt cttaggatct tggttagcaa tcaagcgttt ctcaagtttt tcacgggcag
    20701 cacgccattc agctgtaaca gcattgtaac gctcgtcgtc tgtgatcata ccacgacgga
    20761 attgtttggt gatttgttcg acacgtttgt gtgattcttc aatgatttca gtcttgtcat
    20821 caacgactgg gatatcggca atacccactg tcaatcctgc aagagttgag tggtggtaac
    20881 cgaggttctt catgcggtca agtagggcag aagtttctgt cgtacggaaa cgtttgaaga
    20941 tttcagcgat gatatttcca aggtttttct tcttgaatgg agggttgagc tcaagattgc
    21001 tgatagcttc cttgatatct ccaccaagtg gcaagaagta tttagctgga acaccttctg
    21061 tcaagttggc attgtttggt tcttgcaagt atggtagccc ctctggcatg atatcgttga
    21121 agagaatttt accaactgtt gtaagcaaga ccttatgtct ttgctcttct gtccaaggct
    21181 tgttgaggct gtctgttgcg ataccaacac gtgagtggag gtgaacataa ccattgcggt
    21241 aagccataac cgcttcgtca cggtctttga agaccattcc ttcaccttcg cgaccagctt
    21301 cttccatggt caagtagtag ttacccaaaa ccatgtcctg agatggagta actaccggtt
    21361 tcccatcttt cgggttcaag atgtgctcag cagctagcat gaggatacga gcttctgctt
    21421 gtgcttcttc tgaaagtggt acgtggatgg ccatttggtc cccgtcaaag tcggcattgt
    21481 aggcttcaca gacaagtggg tgcaagcgaa gagccttacc atcaattaag actggctcga
    21541 aggcttggat acccaaacgg tgaagggtcg gtgcgcggtt caaaagcact gggtgttctt
    21601 taatcacttc ttcaaggata tcccagatac gctcatctcc gcgttccacc aagcgtttag
    21661 ctgctttgac gttttgcacg atatcacggg caacgatttc acgcatgaca aatggtttaa
    21721 agagttcaat cgccatttca cgtggcacac cacattggta catcttaaga gttggaccaa
    21781 cggcgataac ggaacgtcct gagaagtcaa cacgtttacc gagcaagttt tgacggaagc
    21841 gtccttgttt acctttaagc atgtggctca atgatttcaa tggacggcta cctggtcctg
    21901 tgattggacg accacgacga ccattgtcaa tcaaagcgtc aactgcttct tgaagcatac
    21961 gtttctcatt ttgaacgatg atacctggtg catttaactc aagcaaacga gccaaacggt
    22021 tgttacggtt gataacacgg cggtaaaggt cattcaagtc agatgaggca aaacggccac
    22081 catccaactg caacattgga cgaagatctg gtgggataac cggaaggatg ttaagaatca
    22141 tccattcagg tttgtttcca gacttgtaaa aggcatccaa aacatccaaa cgacggatgg
    22201 ctttgacacg tttttgtcca gtagctgttt tcaattcttc tttgagttca gcaatttctt
    22261 tttcaagatc tacttgcttc aaaaggtctt ggatggcttc cgcacccatc ttggcaacaa
    22321 atgaaccata accatattcg cgcaagcgct ctcggtattc gcgctctgtc atgatagact
    22381 tgtgctcaag tggtgtatcc ttaggatcaa tcaccacata agccgcaaag tagataactt
    22441 cctcgagggc acgagggctc atatcaaggg tcaagcccat acggcttgga atccccttga
    22501 agtaccagat gtgagataca ggagctttca attcgatatg tcccatacgc tcacgacgaa
    22561 ctttcgtacg cgttacttca accccacagc ggtcacaaac aattcctctg taacgaatgc
    22621 gtttgtactt accacaagca cattcccagt cttttgtagg accaaagatc acttcatcaa
    22681 agagtccttc acgttctggt ttcaaggtac gataattgat tgtttcaggt tttttgactt
    22741 ctccataaga ccatgaacgg actttacttg gagaagctag ggtgatttgc atacttttaa
    22801 aacgatttac atcaaccact atttcttccc tttctattct aagtgaactg cttattcctg
    22861 ttcagcagct tcttctgttg cttccgcttt tgttgctttc tcagcttctt cagcttcaaa
    22921 ggctgcttta gcctcttggg ctgctttttc gcgggctttt tcaaggtcat ctacgtggat
    22981 gacatcttcg tccattcctt catccaagtc gcgaagttcc acttcttggt catcttcgtc
    23041 taggacacgc atgtcaagac caagagattg caattctttg acaagaactc ggaaggattc
    23101 tggaacacct ggttttggaa ttggtttgcc ttttgtaata gcttcatagg ctttcaaacg
    23161 tccgttgata tcgtcagact tgtaagtcaa gatttcttga aggacatttg acgcaccgta
    23221 ggcttcaaga gcccaaacct ccatctcacc gaaacgttgt ccaccaaact gagctttacc
    23281 tccgagtggt tgttgggtaa cagttgagta aggtccgact gaacgcgcat gcaatttatc
    23341 gtcaaccatg tggtggagtt tgatcatgta catgactcca acagaaacac ggttatcaaa
    23401 tggttcacct gtacgtccat cgtaaaggat tgtcttggca tcgctatcca tacctgcttc
    23461 tttaacagtt gaccaaagat cttcagaact tgctccatca aagactggtg tcgcaatgtg
    23521 aataccaaga gtacgagctg ccataccaag gtgaagctcc ataacctgac cgatattcat
    23581 acgtgatggc accccaagtg ggttcaacat gatgtcgact ggagttccgt ctggaaggta
    23641 aggcatgtct tctacaggaa cgatacgaga gacaacccct ttgtttccgt gacgtccggc
    23701 cattttatct ccgaccttaa tcttacgttt ttgagcgatg taaacacgaa ccaacatgtt
    23761 aacacctgat tgcaactcat ctccatttac acgtgtaaag atcttaacat cacgaacgac
    23821 accatcggca ccgtgtggta cacgaagaga agtatcacgc acttcacgag acttgtctcc
    23881 aaagatagcg tgcaagagac gttcttcagc tgaaagatct ttctcaccct taggtgttac
    23941 tttacctaca agaatatcac cttctttaac ctcagcacca atacggataa tccccatttc
    24001 gtcaaggtct ttgagggcat cttcaccaac gtttggaatt tcgcgagtga tttcttcagg
    24061 cccaagcttt gtatcgcgcg tttctgattc gtattcttca aggtgaacag atgtgtagac
    24121 atcgtccttc accaagcgtt cgctcatgat aacggcatcc tcgaagttgt agccttccca
    24181 agtcatgtag gcaacgattg ggttttgtcc aagcgccatt tctccatttt ccatagaagg
    24241 tccgtcagcg atgaaatcgc ctttttcaac gacatcacca acttttacaa gagtgcgttg
    24301 gttgtaagca gtacctgagt ttgaacgacg gaatttttgg atgtggtaaa catccaatga
    24361 accatcttca cgacgaactt ctaccttgtc agcatctgcg taagtaactt taccatcata
    24421 ctgagcaatc acagccgcac cagaatcgtg ggctgcttgg tattccatac cagtaccaac
    24481 gtaaggtgcc tgaggattaa tcaatggcac agcctgacgt tgcatattgg ctcccatgag
    24541 ggcacggttg gagtcatcgt tttccaagaa aggaatacat gctgtcgcaa cggcaactac
    24601 ctgttttggt gaaacgtcca tgtagtcaac aatattagct ggatactctt ggttgacccc
    24661 ttggtgacgt cccatgacaa tcttctcagc aaaggttcca tcttctttca gacgagagtt
    24721 agcctgagct acagtatatt catcttcttc atcagctgtc aaccaaacaa tttcgttcgt
    24781 gacaacacct gtttcacggt caaccttacg gtatggtgtt tgaacaaaac catatttgtt
    24841 caagtgtccg taagatgaca agttattgat caaaccgatg ttaggtcctt caggtgtctc
    24901 gattggacac atacgaccat agtgagtgta gtgcacgtca cgtacttcat atccggcacg
    24961 gtcacgagtc aaaccaccag gtcctaaggc tgacaaacgg cgtttgtgag acaactcaga
    25021 aagcgggttg tgttggtcca tgaactgtga caactgtgat gaaccaaaga attctttaac
    25081 tgcagctgtt acaggacgga tattgataat ttgttgtggt gtcaagactt cattgtcctg
    25141 aacagacata cgttcacgga cattacgttc catacgagaa agtcccaaac gtacttggtt
    25201 ggcaagcaat tcaccaaccg cacggatacg acgatttcca aggtggtcga tatcatctac
    25261 acggccaagt ccttcagcca agttgaggaa gtagctcatc tcagcaagga tatctgcagg
    25321 agtcaccgta cgaaccttgt catctgggtt agcattacca atgatcgtta cgacgcgatc
    25381 tggatcagtt ggagcaacaa ccttgaattt ttgaagaaca acaggctcag tcacaacggc
    25441 tgcatcgttt gggatgtaga caatcttgtt caagtcgcca tccaaatggc tttcaatgct
    25501 ttcaatcacg ctacgagtca taatcgtacc agcttctacc aagatttctc cagtttcagg
    25561 gtctaccaat ggctctgcaa tggtttggtt gagcaaacgt gttttaacat tgagtttttt
    25621 attgattttg taacgaccaa ctgctgccaa gtcatagcga cgtgggtcaa agaagcgagc
    25681 cacaagcaag ctacgtgagc tttcagctgt cttaggctca cctggacgaa ggcgttcgta
    25741 aatttctttc aaggcttcgt ctgtacgaga gtccattgga ttcttgtgga tatctttttc
    25801 aacagtgttg cgaaccaatt cgctgtcacc aaagatatca aagatttcat catcacctga
    25861 gaaaccaaga gcacgaacca aggttgtaaa tggaatctta cgagtacggt cgatacgagt
    25921 gtaggtgata tcttttgagt cgctttcaag ttccaaccaa gctccacggt tagggataac
    25981 agttgaacca tagcccacct taccattttt gtctactttg tcgttaaagt aaacacctgg
    26041 tgagcggacc aactgagaaa cgataatacg ttcaccacca ttgatgatga aagtacccat
    26101 ttctgtcatg attgggaaat caccaaagaa aacttcttgg gtcttgattt cgcctgtttc
    26161 tttattgatc aagcggaagg ttacaaaaat tggtgctgag tagctagcat cgtggatacg
    26221 agcttcttct agcgtgtatt ttggttcctt gatttcatat ccaacaaatt ccaactccat
    26281 tgtgtctgtg aagtttgaaa ttggcaatac atcttcaaac acttccttaa gaccgtggtc
    26341 taggaaagct ttgaatgagt cagtttgaat ttcaatcaaa tttggtaagt caagaacttc
    26401 tttgattctt gaaaaactac gacgggtacg atgtttcccg tattgaacgt catgtcctgc
    26461 caagatgatt ctcctttgta aataagttcc aagccttgtc aatcaggctt ttctaatcgc
    26521 catatggttg taaacccctt atcaccgtgt cctcttgacg aattttcaga atctttaagc
    26581 ctctgttaca aatgctcaaa atcttgaaaa aaagcacaaa aagagcagct aaatctgact
    26641 ttttcagaag atttaactgc tgtgagcctt gtctggacaa tatttcagac aaaacctacg
    26701 acaaatgatt acccatatta taccctattt agctagattt ttcaagggga ttcagtaggt
    26761 ttttggtaat ttttttccca tagaaaactt ggcatcacat tcgaatcacg ctatggtaca
    26821 aaaaactgaa aaaactattg actgaaaatc attttcaagg tataataata aacgttaagg
    26881 cggtatagcc aagtggtaag gcacggctct gcaaaagctt gatcgtcggt tcaaatccgt
    26941 ctaccgcctt ctataacttg atttatcagg tttcaaatga acagaaagcc caatttgaag
    27001 ggcttttttt attttccctc gaataaatac gtataacttt aaaaactttt ggagcgagtt
    27061 tgtggcagag ttctttccat ggcataattc ccttttgaaa tcaattgaaa aaagcttcgt
    27121 cacgcctggt gacaaaagcc tctagtttat tctgtttctt cttctatttc gacttctgtg
    27181 atttggactt ttaccttggt aacacgtcca ttcttcactt tatcattggt taagataaac
    27241 tgtttgtttt ggctgaccaa ttcatagctg agtttctcag tcgttggaat ggttcccact
    27301 cctgtcaaat aataaccagc gatagtatca acgtcatcac tttccagttc aacattaaag
    27361 taattattga aatcattgag ggtcatagtt ccttgaacaa tgtaggtatc ctcgccaatc
    27421 tgatgaacat ctattgctgc tttgtccgtt tcatcatcaa tttcaccaac aatttcctct
    27481 aagaggtctt ccaaagtcac caaaccagcc ataccaccgt attcatccag caagattgcc
    27541 atttgtcttt gggtatttcg cagttctttt agcaagtcat ccacaaaaat agtttcaggt
    27601 acaaaaagtg gatcttgtaa aattctcttc caaacaatat tgtcaaaacc gtccacaaag
    27661 cctgccttaa ggagactctt ggtgtgaatg attccaatta cattgtcctt atccccatca
    27721 taaaccggga tacgagaata attttgtttt aaaatacttt ggataatggc ttgactatca
    27781 tcctgaatat ccaccataaa ggcatccgtt cgaggaacca taacctctcg tgccatcagt
    27841 tcatcgagcg aaaagacacc ttgtaacatc tcaatctcat cagcatccaa tgtttcttca
    27901 ctatttgtca gcatataggc aatttcatca cgggtcatct tttcatccgc atcatcgaat
    27961 gacataggag tcaaatggct caagaaattg gtcgaagcag ctaaaagcca aacaaaagga
    28021 ctgactagtt ttccgatccc aatgataacc ggcgctgtac gaattgccaa ggcatccttt
    28081 agattaagag cgattctctt aggatataat tccccaaaaa cgatggaaat ataggtcaaa
    28141 aatgccaagg atagaaaagt tgccacggct tgtgctgttt cgccattccc aagccaagag
    28201 gcaatcacac gtcctagagt atcagttaaa ctcgcccctg ataagattgt aatcagggtg
    28261 attcctacct gaatggttga taaaaagtgg ttaggatttt ctagtacctt cagcaggcgg
    28321 atgtagcgtc tgtctccttc ttccgccttt tgttcaactc gggcacgatt aagagaaacc
    28381 atggccattt ctgtggctga gaaaaaggca tttaataccg tcaagataaa caataaaaca
    28441 aattgtagca acaaattctg actacttggg tcttccatag ttcttctcct aaaatagtat
    28501 cttatccttt attatatcac aaaatttaat ccaatacata ttattttttt cttagcgtaa
    28561 cttccatctc tctcccaaaa ctggaagtta gtctaagacg gatttccact gatttcacgc
    28621 gtttttctca tactaaaagc taggttgtgg gaaactggaa gttagtttta gaagttacta
    28681 ttttttctta gtacctgttc tagccctgct taaactagta aaaagaactc ctacatccat
    28741 aagtgacata ggagtttagg cttatctttt actgagttac agttacttct ccagttcggt
    28801 aatccagttg aagtcccttt tgaacattgg gaactagaac attgaattcc aattctccat
    28861 ccgaagactt ggcttcaatc tgtgaagctg agggaactaa atcctcgttt gaagcgtagt
    28921 aaagggttac acggtaacgg acacgcttgt tttggtccaa ggctttacgc accttgcttt
    28981 catagtagtt ttgaccagtc gaatactcgg cttgtgcctg atttgcccag gctgtctgaa
    29041 cagcaatgtt tttaggattg cttgttgagg catcaaaacc atccaaacca ccgattaagg
    29101 catagcctaa caaatgacct ctatcgactg catgggtata agagcccttt agattcttga
    29161 cctgatgcca acctggagga gtccaagaag ttgaaccatt cccagtttct ttacgattct
    29221 tgtactgacg agtggcctta gacaagaggg cattagctac ggttggaaca gtttccttac
    29281 ccactgtctt tgttttattg tcagcgtagg gcttacttga aaccttggca tctagatttg
    29341 ttttattacc attgacgata aaagcacctg agccattcca ctccagactc ccctttattt
    29401 gactcttgac tgcgtctgtt aagacactct ctgccaatgc ttgactagga gcttcaggcg
    29461 cttgtttttt ctgactaagc ttggttttag gactattagc tgtcgactgc atctgcttga
    29521 tataatagct ccctgtagac aaaagcaata acactagcag tccgattagt gtctgtcttg
    29581 tttttttgtt catatttctc cttatcttta gaaaaaggct ggttctccag cctaatttcc
    29641 tgtaaattta tgaatcaatt cctgccattt tgctggagac aggatagccc atgaatcttg
    29701 acccttgccc aagattccat aacctaccat taaaccgatt cctagagcca gagtacctaa
    29761 aatcagtact atgatgacta aaagtaaacg cttgactacg tagcttgatt tcttattcat
    29821 cttcatcact tgcctcaatc cgctgaatct tagcacctag ctgcgccaac ttctcatgga
    29881 aaccgtagta acctctatcc aagtgaacca atttaccgac cacagtttct ccctgtgcta
    29941 ccaaacctgt caaaatcaaa gctgcactgg cacgaaggtc agttgaaaga acttctgctc
    30001 cctgcaaagg ctgtccacca acaatacgag ctgtatcacg gataatctca gaatgcaagc
    30061 ccatgcggcg catctcttct aggtgttgga aacgattttc aaaaactgtc tccaccatgg
    30121 ttgattcgcc ttttgcaact gtcatcaagg ctgtaaattg agcctgcata tctgttggaa
    30181 atcctgggtg gggcaaggtt ttcacatgaa cagcttttag attttccagt tgagaacgaa
    30241 cacgaattcc ttcgtcttct tcaattactt caacacccat ttcaagtaac ttggcaatca
    30301 agggacggtt gtgctcccag acagcgtctc gaatcaagac atcaccacca gtcatggcag
    30361 cagctaccat aaaggttcct gcttcgatac ggtcctggac tacattgtga gtcgtaccat
    30421 gaagtttctc aacaccagta atggttatag tctctgtacc agcacctttg accttggctc
    30481 ccatttcatt aaggagaatg gctaagtcaa caatctcagg ctcacgcgca gcattctcaa
    30541 tcactgtcac cccatcagcc agagtcgctg ccatcatcaa gttctgcgtt gcaccaacac
    30601 ttggaaagtc catatagata tgagcaccat gcaagcattc tgcctttgct tcgatgtaac
    30661 cagctgtctg actaatctta acccccatag cttccagacc tttcaaatga agatcaatag
    30721 gacggctacc aatcgtacaa ccacctggca tggatacctt ggcatgaccc acacgggcaa
    30781 ggattggccc taatacaacg atggaggcgc gcatcttact gacatacttg taaggggctt
    30841 cctcagtgat gtcgccagta gcatccacct tgacaagatg agcttcctca tcaaagtcaa
    30901 ccttggcatt caaaccacca actacctgat tcataataaa gacatccgac aaaatcggaa
    30961 cattctgcaa gacggtcttt ccttcacttg ctagaatagt cgctgccaac aagggtaaga
    31021 ctgcattttt tgctccctcg atcgtcacgc ttcctaccag acgattatcg ccaccttgaa
    31081 ccacaatttt atccatactc gtttccttta ctcttgattt tataataatc ctctaagcgc
    31141 ttcttgccac aaatgataca ggctaataaa gaaagaactc aggatgtagc ctatgacaat
    31201 gctaaaaaag gctactaata cacgaacttt tcttgtattc tcagctgtca cttttaaaac
    31261 cttttcccat ctaacaagat cctttaaaag gtaaaaactc acataaataa agagcatatg
    31321 actacttaga gtgaataata attgaaccat ttgactatta taccatcttc tctgtttgtg
    31381 cgctagtttg gaaacttcac tcaaaaatta gggattgttt ttcaagtaat caatcgcatc
    31441 ttgtagggtt ttaacgggca cgattttcat atccgtcttg attgttttag ccgcttctag
    31501 ggctgtttgg tagttgtttt tcgcgtccgg atgcgccttt tgttcttctt cgctaacagg
    31561 gttattagga gcaaagaaaa tagcagcacc tgccctagcc gaagctacaa ccttcttatc
    31621 aatacctcca atgtctccca cattaccatc gcggtcaatg gtacctgtac cggcaacaat
    31681 acgaccatta cgaagatctg ggtgagctat ttgagtatag atagctagac taaacatgag
    31741 accagcactt gggccgccaa taccagctgt tgaaaagcta attgggacat tgctgattac
    31801 ctctgtacga tcaatcaagc cgattccaat cccatttttt ccattttcca aggtgataat
    31861 cttcccttct gcagacttgg tttgcccatc ctcttcatag gtgaccttga cggaatcccc
    31921 taatttttga gaactgacgt aatcaatcaa gtctttggaa ctatcaaagg tctgatcatt
    31981 gactgctgtg actgtatcag agatattgag aatcccttta aaggttgaat tatccgtcac
    32041 attcaaaaca taaactccaa agtacttgag ttcgatatcc ttaccagctg tttttagtcc
    32101 ttgatacttg gccatatttt gcgatgtttg catgtagaat tgattgattc gcataaattc
    32161 aacatcggaa gaaccacctg tagtctcctg agcactacga atatctgtaa aaggtgtcaa
    32221 ccaagcataa atcatatgag ctaaagtagc atgttgaaca ccaaccgtaa cgaattgata
    32281 ggcaccagct tccttatctt ctgtgtcatt tactttaagg acttggcgaa tatcttccga
    32341 accacctgga acctctatat aataaggcaa gggcactaca aatgccaaga aagtcacaat
    32401 caaggccgca atgacgtata agggccatct aatctttttt ttcatttctt atttcctcca
    32461 aaatactctc gggaacatag caggcaatat cctgaccaaa cttcaaaagc tctctaacgc
    32521 ctgatgaact gatatagaga tgttcaggtc gactatgtaa ataaatagtc tctatatcag
    32581 aagacagctg atgattgtag taatcaaaac tggcttcata ttgcaaatcc gacgcattcc
    32641 tcaaaccacg cactagacaa gtagcaccca atctttttgc aacatcgacc accaattcat
    32701 catgagaagc cacgacttca acattttcca aatgtcccaa agccttttct agcccccgtt
    32761 tacgattttc aagagggaga aatccttgtt tgtggggatt aaaaaaaata cccacacaaa
    32821 gcttatcaaa aagtctgctc gcccgttcaa tgatatccag atgcccattt gtcatcggat
    32881 caaatgagcc tgtgaataag ccaatcttat ctgacataga ctgtcacctt actaattcca
    32941 taaatctttt ccttccagat acccagacag gcaatttctt ctggaagttc aacggtctta
    33001 tccgtctcgc acacaaccat aacatcttca gaaaaaagct ctctctcagc cattttttca
    33061 atatctgcta cgatttgttc cttggcatag ggagggtcta agaaaacgag gtcaaattcc
    33121 ccagatacct gttccaatgc cctttctgca tccattttga ggagttgaaa ttttccaact
    33181 tccttggtca tctggatatt ttcagccacg atggtctgag ccttacggtc tcgctccacc
    33241 aaaacagcac tggacatgcc acgcgatact gcttcgatag ataaaccacc actacctgca
    33301 taaaggtcca agactcgtcc cacttcaaag tagggaccaa tcatgttaaa aatggctccc
    33361 ctaaccttat ccgaagtagg tcttgttgtc ttgccttcta gtgtcttgag gggacgtccc
    33421 ccatagattc ctgatacgat tttcatactg tttattatac caaattatag acaaaaagag
    33481 aaagaaaacc gaaccttgcg gttcgattct ctacaaaata ttttcgtaag tatcgcggac
    33541 ttcttgaggc caaacacttg tttgcacttc tccgatgtgt ctcttgcgaa gtaggaacat
    33601 ggccatacga gattgtccaa ttcctccacc gattgtcaat gggaataggc cattcaacaa
    33661 agacttgtgc cattccaatt ctaagcggtc ttcatcacct gtaatttcca cctgacgtct
    33721 aagagtttct tcatctacac gaattcccat agaagacaac tcaaaggctc cacctaaaga
    33781 ctcattccag acaagaatat caccatttag acccttgtag ccattctcag actcgcttgt
    33841 ccagtcatca tagtctggtg cacgtccatc gtgcggttta ccatctggca actcgccacc
    33901 gataccaatc aaaaagacgg ctccaaattc tttacaaatc gcattttcac gttctttcgg
    33961 tgtcaagtct gggtagcgtt ctaccaattc ttctgtatgg ataaaggtga tttgttttgg
    34021 caagatagac tcgatgtcat agcgggcttc aacagctagc tcagtcaggc gaatagcctt
    34081 ataaatcttc tcaactgttt cttttagata aacgatgtta cgcttaccat ttgggataac
    34141 cttctcccag tcccactggt caacataaac agagtgggtt gcatccaagg aatcctcatc
    34201 tggacgaagg gctttcatgt ggacaaagag accctctcct tcaccaaagc caaaacgagc
    34261 caaggtgtgg cgtttccatt tagcaagtga gtgcaccact tcataagtag catcagggat
    34321 ttggagaacc ttgaccgata ctggattttc cacaccagac aggttgtcct gcattccgtc
    34381 accgacctta ctcaagatag gaccttgaac ttcgacaact tctagcttat ctttcaaata
    34441 ctgggtaaaa gtgtttttga caaaggaaat ttcttcttgt tgatggataa aacttttctt
    34501 catagacaac tcctcaaaaa tttaataaac gtattataca cctatttcca agaaaagaaa
    34561 agagaaaatt taaaaaaatc agtgtcactc aaaacactga tttcatagac cttttagtca
    34621 ttgcgaccaa agatacgtag aatacttagg aaaagattga taaaatcaag atagatgctg
    34681 agagccattg acaccaccca gcctgtcgct acacgacctt gtgattgttc ataggcgagg
    34741 cgaatctttt ggttatccca agcaatcaac cctgaaaaaa ccaaaaccat ggctacgcta
    34801 atcatataat caaagaagcc actagccaag aaaatattga ctaccatagc aatcagtaaa
    34861 ccaatgagag ctgccatcat tgctcgacca atcccactca agtctttctt ggtaaaaata
    34921 ccaactgccg ccatgacaaa gaagagaagg gcgctcgata caaaggcaga taaaactgta
    34981 cccggagtat agaaagccac cacaaaacta agggtaaagc cgtttaaaac ggagtaaagt
    35041 aaaaatactg gaagagcagc tggactattt cttgaagcca tgctactggc aacgaaaacc
    35101 aaagccaact ccgcaaaagt agcaatggtc aaccagagac gcccctgcat caacaagtaa
    35161 accaactgag actgaaagac cgtcaacata aggcctgata ccaaagcaga tagtccgatt
    35221 cccagaccaa caaaggcata aaccttagcg taaaattgat tgagacctgc acggtcatgt
    35281 ataatagtgt gattcatcct tttctccttt tctgtgaaca tctttccttg attataacaa
    35341 aaaaagttaa aattagctta aatggaagat taaaataccg gctgcaactg caacattcaa
    35401 actctcggcc tgccccttca tgctaatatg gaccaactgg tctgcacttt cagccataag
    35461 gggactaatt ccttgaccct catttcccat aactagtaca aaattttcta tagaaggcag
    35521 ttctctgtaa tcaacagaat ctttagatag ggttgttgct agcactggga tagctacctt
    35581 tttagtttcg tcaagaagcg cttgactagt catccggtaa atgggcagat ggaaatgact
    35641 accttgcatg gaacgtaggg ttttgagact gtagatgtct gccgacttat ctgaaacaat
    35701 cactcctgta aaacctgctg catccgcagt tcgaatgata gttcctacat taccaggatc
    35761 ttgcacatct tccaaaaaca agaacttgcc ctgactcaaa tcagcttgtc ctacttcttc
    35821 tttttgaacc acggcaacaa ttccctgtgg agtctgagaa tccgccaaat ctagcaaaat
    35881 atcctctgaa atccagacag tttgcggaaa agcagctaac tgatttcgat aattttctag
    35941 ggcaaagatt ttctcaatcg tcactccagc ttggacagct tcttcaaaca agtgccagcc
    36001 ttcaatcaaa taggcagact tgcggtattt tttttggtgt aatttcttgg cattttttac
    36061 cacagaattg gcttttgagg ttataatagt catagaaata ttataacaca atcaaagggg
    36121 tttggtatgc aaaaggttag aatgattgcc caaggtaggg tgcagggagt cggctttcgt
    36181 tggggtgttt atagcctggc ccttgaaatc ggtggcatca caggtcgggt atggaataac
    36241 gacgatggca cagtggaaat cttagcccaa gcagactcat ctgctatcat ggcaaaattt
    36301 atccaagaaa tccgaaaagg accgacacct ttttcaaaag taagctactt agatgtcaaa
    36361 ctaagcaact ttcctcccta ctctgacttt aaaatcgcaa attaggtctc cagaactatt
    36421 gtatattttg taaaaaaaca gtagaataga aaggtataat ttttaaagaa ggaataaaaa
    36481 cagtgaaatc tattaaacgt tttgcattct cggctatggg agtggctatg ttgcttgtct
    36541 tgactggctg tgtcaatgtc gataaaacca caggtcagcc aacaggattt atttggaata
    36601 cgatcggagc gcctatggct gaagccatca agtacttcgc tactgataaa ggtctaggct
    36661 ttggtgtcgc tatcattatc gtaaccatta tcgtacgctt gattatctta ccacttggta
    36721 tctaccaatc atggaaggca acgcttcact ctgaaaagat gaacgctctc aagcatgtcc
    36781 ttgagccaca ccaaacacgt ctcaaagaag caactactca agaagaaaaa ctcgaagccc
    36841 aacaagccct ctttgccgct caaaaagagc acggcatcag catgtttggt ggtgtaggat
    36901 gtttccctat cctccttcaa atgcctttct tctctgctat ctactttgct gcccaacata
    36961 ctgaaggggt tgctcaagca agctacctag gcattcctct aggttctcca agtatgattt
    37021 tggttgcctg tgctggtgtc ctttactatc ttcaatcgct cctttcactt cacggagtag
    37081 aagatgaaat gcaaagagaa caaatcaaga aaatgattta catgagccca ctcatgatcg
    37141 tcgtcttctc cctcttctca ccagctagtg tcacacttta ctgggttgtc ggtggtttca
    37201 tgatgattct ccaacagttt atcgtcaact atatcgttcg tccaaaactt cgcaaaaaag
    37261 tccgtgaaga actagccaag aacccaccaa aagcaagtgc tttctctaaa ccaagtggac
    37321 gaaaagacgt tacccctgaa caaccaactg ctatcacaag caagaaaaaa cacaaaaatc
    37381 gcaacgctgg aaaacaacgt tcgagataag aagaaaaagg cttagctgat ttgctaagtc
    37441 tttttgcaag tcaaaagccc gatgtttcca tcaggtttct tttctatcca tgtacacgtt
    37501 tcatataatc ttgataactt tcggtatcca tgagtttttt agcgttcttg acgcgatctg
    37561 ctgttggtgg tttgactcct tcgagggaat atggaattcc aagttcacgc cacttgaact
    37621 cacccatggt gtgataaggt agaatttcaa acttatcaac atttttgagg gtcttgacga
    37681 acttaccaag ttcaatcaag tcatcatctc tgtctgtcaa tcctggaact agcacgtggc
    37741 gaatccagac aggttttcca atatctgata gatactgggc acaagccaag atatttttat
    37801 tagtttggct agtgacaatc ttgtgctgtt cttcgttgat ttccttgata tccaaaagaa
    37861 ccaagtcagt gacagccatg agtttgtcaa acttctcaag gtaacgtggt ttattacgga
    37921 aaggaagagc acaggtatcc aaggtacagt ggattccttg ttccttagcc ttggtgaaga
    37981 gagcaatcag gaaatcaatc tgcaagagag cttctcctcc actgactgta atcccaccct
    38041 tatttcccca gaaaccacgg tagcgcaagg cctctgtcaa gacatcatct accgtccgtt
    38101 cacgtgactt attggactcc atagcccaag tgtctgggtt gtggcaatac tggcaacgca
    38161 tgtgacagcc ctgcaaaaag acaataaagc gaataccagg cccatctact gacccaaagc
    38221 tttctgtcga atgcaccatt cctgtcactt gtccataatc aattgtttct tcagacatat
    38281 cgttaccttc cttgaaaacg ttttatagtt ttattatatc acgacttgaa ggaaaaacaa
    38341 gatttttgcc tattttttag aaagcaatct gtttttcaat ttcattttca atctattatc
    38401 agattattct tcaaaatcga agccatataa ggttgcaaaa tagtcctcag ggcgctctgc
    38461 acggcggatt tgacgggctt taccttcttc ggtatagagg atttccgcag aacgtaattt
    38521 ggcattatac tggtagccca ttgaaaatcc gtgggcacct gtatcatgaa ttaccagcaa
    38581 atcaccgatt tctgtatgag gcagttcgcg attgactgca aatttatcat tgttttcaca
    38641 gagtgaacca accacatcta ccgcttcagc tggtccatct ggatgggtca cgttgctaat
    38701 atgatggtaa gctccgtaca tagctggacg catgaggttg actgctgagg catccacacc
    38761 tagataggta cggtaggttt ccttcttatg agtcactctt gtgactagag caccgtgagg
    38821 tgccagcata aaacgaccca attcggtgaa aatcttgacc tgaccaagac ctgctgacgt
    38881 aagaacttct tcatacacct tacgaactcc ctcaccaatc aaggcgatat cgttcggctc
    38941 ctggtctgga cgataattaa caccaatacc gccagaaaga ttgataaagt ctagcgaaat
    39001 gcccaacttt tccttgattt caacagccag ttcaaagagc tgacgagcca actctggata
    39061 atagagatgg gtcacggtat tggacgctag gaaggagtga atcccaaaag tcttggctcc
    39121 tttttccttc aagatagcaa aggcttcaaa gagctggtcc ttggtcatgc caaacttagc
    39181 ctccccagga ttgtccataa tgtctgtccc cagttcaaaa acgcctccag gattataacg
    39241 acaagagatg atttctggaa tgcctgctac tctctccaga tgttcaatat cttcaaaggc
    39301 atccaagtta atggtcgcac ccaattcacg tgcataggcg tattccttgt ctggcgtgtt
    39361 gttggaagag aacataatct cagaacccag aaagtccagt ttatggctca tcaaaagctc
    39421 tacataacta gagcagtcca caccacaacc ttcttcttgg agaattttca aaatagctgg
    39481 agttggagta gccttaactg caaaatattc cttaaagccc ttgttccacg aaaaagcttg
    39541 gttgacggct cttgccttct cacgaatccc cttctcatca tacaagtgaa agggagtcgg
    39601 gaactcggca acaatcgctt ctaactcttc tctattgata aatggtgttt tcataagctt
    39661 ccttctattc tgtttgcaaa gaaaggctgg gacaatcgtt ccaaccttta gttctatctc
    39721 ttatttatct tcgtcaaaga tattgccaac ctcaacatcg atggcttggt tcttgacatc
    39781 aatattggtt accttcaaga gtgacttgta gtcaaactgc ttttcacgtt cttcttgggc
    39841 attgtccgca aagaccgcta cacctgccag ttctgagtcg aactcgtgca agagactaat
    39901 cataccattg accgttccgc cacctttcaa gaagtcatcc acaatcaaga cacggctgcc
    39961 tgccttaaga ctacgttttg aaaggaacat tttctcgata cggtcaccac ttgaacctga
    40021 aacatagttg acgctaacag ttgaaccttc ggtaattttc aggtcacggc gcacaatgac
    40081 aaaagagaca ttgaggacat tggcaactgc atttgcaagt ggcacaccct tagttgctac
    40141 ggtcataacc gcgtcaattt tttggtccat aaagcttttg gcaataatac gaccaatatt
    40201 tttcaagatg gctggtgtgc taagcaaatc agacagatag atataaccac ctggcaagat
    40261 acggtcactt tctgacaact tggtacgcaa gtcttcaacc atttccttag catcctgact
    40321 cgaaatagac ggtgtgaaga tgacacctcc gccagcccca gtcactgtct ggatatgacc
    40381 gatttcaatt tcctcaaagg cgcgtttgat aatgacgata tcttctgaga tggatgattt
    40441 agcagactca tacttttcag caaaagtatt gagactagtt agtttataag gattattaat
    40501 caaatagttg gaaatgacaa ccatccgatc acttcttctt aatttcattt ctatttccta
    40561 ttccattttt attcaaaaaa tcaaaaagca aactagaaag ctggtcgctg gtggttcaaa
    40621 acactgtttt gagattgtca atagaactga caaaccctgt aatatacctg catatataca
    40681 tacgacaagg cgatactacc ctagtttgaa gagattttcg aagagtattc atttttgtct
    40741 tttacttatt ataccatatt cacataaaaa aacgaacatt cttatcctaa aaaatgctca
    40801 tttttcttaa attatcaatc taaatctggt ttatagaagg aacgattatc catagcgaag
    40861 attttattgg tcatctctcc tttatccacc aaagccagag ctgttgacat catcatcatg
    40921 cttgcatcca gattgtcaat catatggata atctctgcct ccataatacg tggacggact
    40981 ggacttccat attcaagcaa gccgtggtga ctgaggatga catgacgaag caaaacgact
    41041 tcttccttgg tatcatcgat gccgagttcc ataactgtct tggtaatttc gctatcaatg
    41101 agagcgatat gtccaagaag attacctcgc actgtgtact ctgtctggtc tggccccgtc
    41161 aactcgataa ccttagctaa gtcatgcagc ataatccccg catagagcag gctcttattg
    41221 agctgaggat aaacttcgct aatagcgtct gccaaacgta ccatggtcgc cgtatgataa
    41281 gccaaccccg tttcaaaggc atggtggttg gtcttggcag ctggatagga gtagaattcc
    41341 ttatcatact tggtgtagag atttcggaca atccgttgcc agacaggatt ttcaattttg
    41401 aaaatcattt gcgacatgta gtcacgaatt tccttgacat caactggtga cttgaccttg
    41461 aaatcggctg ggtcattggg ttcaccagct tgaggcaggc ggagagtaat ttgattgact
    41521 tgaggggtat tgttataaac ttctcggcgt cctttcatgt ggacaacctt acctgcggta
    41581 aaggcctcaa tgttatgagg ttgggcatcc cagagcttcc catcaatctc gccactatca
    41641 tcttggaagg taaaggctag gtagtttttc ccagctcgag tttgcctcag gtcagctgat
    41701 ttgattaggt aaaagccttc aaatagctca tcttttttca tgtgactaat cttcatattc
    41761 ttcctcattt tcttgaaaat ggagtagatc aagcgcaggc tcaccttctg acaactcaat
    41821 gtgacggagc gtccgctcga tagctatggt acgacggttt aataattcat caatattgcc
    41881 agaggcatgt tggagatgtt tttgtgcctt gaccagaata ccaccaaact tgccaaactc
    41941 ggtcttgaca ctggcaagag tcttgctgat atggtcggca ctcttttgga tattaagggt
    42001 cttgaaacca actgatagag agttaagaag ggctgatagg gtacttggtc ctgcaacaat
    42061 aatctgttct tcccgtctca aatcatcaaa gaagaccgga ttgcgcacaa tctctgaata
    42121 aagaccttct gtcggaacaa acaagattcc aaagttggtt gtccgaggtg gtgctatgta
    42181 cttgttccta atatccctag caaagcgctt gacgcttgct aggagtgact tacgacagcg
    42241 ttcaatctca tccttgtcac ctgtctcata ggcttcttcc aagcggtaat aatctgccag
    42301 tggaaactta gagtcaattg gcagatagac gtattcttgg tcgccttgtc cgggtaactt
    42361 gatggcatac tccactcgtt cactagagtt ttcaaccgtt gcgtattctc gttcgtactg
    42421 ggcaggtgtc atgatgtctt caataatttg ccccagttgc aattctccca gaatccctcg
    42481 cgtcttggtt ccagagagaa ccttgttaag agctccgaca tcacgggcaa ctgtctgcat
    42541 ttctccaagg ccacgattga cagactccag ttgtttagaa actgtctcaa aggaagcctg
    42601 taagcgtgtc tgcaaggtct tttctagttt ttcctcgacc gtctggcgca tttgttccaa
    42661 acgttgctca tttgattcct gcaaggcttg gagacgttgg tctgtcttgt ctctagtttg
    42721 gaggagatta tctgtcattt cttgacggac ttgggtcaga ccttggtgca attcaatccg
    42781 cacttcttgc aaacggtcgc tgacaaccac ttccaaatct ttttggtcta actggctggc
    42841 ttgtctggct tggtcaaagc ggtaatccaa ctggtctgac aaatgatctg cctgatcctc
    42901 caagctctta cttaagtgtt tctcctgcct atcctgcctt tgccaaatca gaaagagacc
    42961 agctagattg gcaattaata atagaataag taaactttcc atcctacctc ctgtccttgc
    43021 tatgcagtac gaccacataa ccatctgggc aagttaccga cacttcccta tctatatatt
    43081 cgttagaagc gtacactttt ttaaagaaaa aattttcctc tgtcaactca tacttggctc
    43141 caagaatagt cagctggcta tcccgaactg gcataaaggc tagatagtcg tagtctgaac
    43201 gaggttctag ctgactgatt ccttctggac aataagtaat caagttttgc ccatcctcaa
    43261 tttctatttg atgcatatag gatgccaact taggattgct aggcagaaag acattggcca
    43321 acatatggtc aatacggcca cccaaggcac cgaaaatagt aacctgagcc tgaggatttt
    43381 gcttaaagat ggtcaagaga gccaattcca aatcagtatc atctttttct ggccgggctt
    43441 ggacaaaata ctgggcacgt ttttgaatca cctgtcgctc ttcttccgtc acagaatcaa
    43501 aatctccgac tgctagagca agaggcaaat cttcttccaa gatccagagc gagcctcgat
    43561 ccacaccaac aaaaccatca aagtccgtcc gataatgacc gcggtctccg cctgcaaaaa
    43621 cggcaaccct agtccagttg ttttctgaga gtttgtactc gctcattgac atctccctta
    43681 aagacatagg aacctgctac aaaaacggtc gcaccagttt ctttggcttg agcaatagtt
    43741 tgatcatcaa tcccaccatc cacttcaatc tcaaagttca aacctttttc atcacgaagg
    43801 gcaaccagct cacgaacctt atccatggtt tctggcagaa aggcttgacc accaaatcca
    43861 gggttaactg tcatgactag gacttggtca actagatgaa gcacatgctt aatcgcttca
    43921 actggtgtac caggattgat aacaactgat ggcttgacac cgagtgaacg aatcttttgg
    43981 agggcaccat gaatatgagg cgttgcttcc acatggatac taataatatc cgcacctgca
    44041 cgcacaaagt cctctaaatg atgctcaggg tttgacacca tcaagtggca gtcgaaaacc
    44101 atcttactat gaggacgaag agcttcgacc acacccgcac caaaactgat ttgcggcaca
    44161 aaatgaccat ccataatatc gatatgggca tactctgccc cagttgcttc taggcgttta
    44221 atttcacgtt caaagttggc ataatctgct gccagaattg acggagcaat cttgtattga
    44281 gacataggtt tctccttatt ttggaatttt tttgctgact tttttatagg tttctctgcg
    44341 attttcaatt tcgctgagga attgcaggta gttgtcaaaa cggaagggcg caatggtacc
    44401 ttcttcaact gctggcttga cagcacagga cggctcatgg gtatgggtac aagtacggaa
    44461 tttgcagtct cgactgacag tggcaatctc tggaaaggcc tgattgaggt cttcagccgt
    44521 tgatacttca taatccagtg atgaaaagcc tggtgtgtct gcgattttac ccccattgag
    44581 gttgtaaaaa ctaacagctc gagtggtatg acgcccgcga cccagactgt ctgaaatttc
    44641 tcccgtttca agattaagat ctggtgcgat tttattgaga agagttgatt tcccaacacc
    44701 cgtctgcccc ataaagaccg taaccttgcc tgttaacaaa ggcaggagtt cctctttact
    44761 ggtcacaaag tcatatccga tgtcaccata ggtctgctgg taaaaatcca gttctccccc
    44821 gtcttccaac aaatccattt tagaaatata gacgatggga tgaataccct tatgctccaa
    44881 aagaaccaag aaacgatcca gcaaattgct gttaaaatca ggttccttga cagacatgat
    44941 tactacagct tggtcgatat tgacaatagg cggacggacc agactgtttt tccgttcgtg
    45001 aatcttgaga atatagcctt ctgaattttc ctcggcagag aaatctaccc agtccccaac
    45061 gtaaggggta tgcccttttt tacggaaatt cccacgcgcg cgtgtttgat agacttggcc
    45121 atcactctct acatagtaga acccagccaa ggctttaatg atttgtccct gcaaaataac
    45181 tcctttaaaa ttgttagttc cattataaca aaaaaaagct attttagctt agcttctctt
    45241 tacaatttcg ataagtctat tctaaaagaa aaagatacac tcacgcttta gataccaagt
    45301 tttatacaaa aataacgaat ctacctcctt atataaaaac aggcgaacag agagcctaaa
    45361 actccctgtt cgctttactt tttctgtgtg taatagaaaa aacctaataa agcaagacaa
    45421 aaaatatgaa tataaactcc atacatcatg tggggaaaga cagactctat aaaatagcct
    45481 ggtagataga tgattggaaa ttgatagaaa tcataggtcg ttagagatag taaacaaaga
    45541 acatacataa atactgctac aattagaacc catccttgct ttcgccatag aaaaatcatc
    45601 aataagaaca gacaaagtag atagattgac caatttcgca acataaattg aaaaccataa
    45661 atcatacttt cagtcgtttc tccatcaatc acatcaacta ctttatagaa aggtaaacaa
    45721 tagctcacta aaaaaagaag gggactaatt atttttttca tattctctac catggattat
    45781 tttgcaagta agctgaccat ctatctccat atcgaatcaa attaacagta taattagtta
    45841 ctacccgatt ttcacctggt ccaaccttag aaaaacctgt gctatactcc caactacttc
    45901 ttgcacctgg atgaaaccat ttttgctggg atgaatacca accaccttgt ctaacttcca
    45961 cacttgaaga ttctatttcg atatcccttt catggaagtc aactcctcca cttattttag
    46021 taatattttt ttgtacacct ccatcataac tcacccagtg aacaacagta tacaattcca
    46081 cactataagt actatcaaac tgactttgat gagtagtaat gttttgagta taagcgtgca
    46141 ctttctgtat accaaaaata tggtctaata tattcgttgg tttagcaagc ttattgtaac
    46201 cctcttcaac aaaagtagcc ttctctaaat cagcggtata aacagttttt ccttgtacta
    46261 cttgattgtt tatagaagta atacggctat ccacaacaat gggggccacc atatctccat
    46321 cgctagtttc tattttagct gcctctttca cctcatatac aactcgattt tcttcagact
    46381 gaatcacttc taccccattt ttttcaggta tagataatct agtttgccca ttagaaaccg
    46441 ttatatttgg accaatattc ttctcatttg cataagtcgc aataggaaat cctgatagaa
    46501 ccactgcaca cataaggcta ttgactaatt tttttctcat gaatatatcc ttcccatttt
    46561 caagaggagt aaaaatagct aatataagat gaacgtttcc ttatatttca gaataggata
    46621 tctatctgaa agcaattcac actcctatag ataactgttc caaatcttgg ctacatatat
    46681 ttcaatagtt tgctacaaaa aactcctttc taagtctatt gaacatgccc ttattcacct
    46741 actaatatat cacaaaaata aataaataca agactgtttt ttctactagt tttatgatag
    46801 aatagtattg ttagggtaaa tttcatatat aaatgtcgag tgaaaaatcg atggagcaga
    46861 aatcggtaga caaagactcg atttcgttgg tccagcccca caaggctaat caggtatttc
    46921 acgagtttaa aaatctaatg aagtgcccaa tcaactactg attcattgat ttaatacgaa
    46981 ctatagtgga ttgaacctag aaatagtaca ctaaggattc taaaacgttt ctagaaatta
    47041 atttgacttt cctaatctct ttgttcatat cttatttcaa tctactatat ataccaaaat
    47101 gctaggacaa aagtctttaa aatcagagaa acgcatatta tcaggtgttg gaaaaccttg
    47161 ataatatgcg ttttatcata gaaagatcta cttcattttt tactaaaatt aagtttttgt
    47221 ccctgcatct tagagtcctt gccctttaag tgcatctgct aggctggcaa actcttccaa
    47281 gctgagagct tccccacgca cacttggtga caagcctgct tggtccaaag ccttggtcag
    47341 cttgtccttg acctcttcag tcttgccaaa gtaacctgtc aagttattcc acaaggtctt
    47401 gcggcgatgg gtaaaactag ccttggaaac cttaaagaaa aagttctcgt cttccactgc
    47461 tacagctggc actggacggc gcaccatttt caagatggct gagtctacat ttggtgctgg
    47521 cacaaagact gtacgcggca cgataaaggc aaccttggct gtcatgtaat actgcacagc
    47581 gatagacaag ctaccgtaag ccttggtatt tggttgagcc gagatacggt ccgccacttc
    47641 tttttgcatc atgaccacaa actcactaaa aggaatgcca ctctcgatta agtgcatgag
    47701 aataggcgtt gtgatgtagt aaggcaaatt agccactacc ttgattggca ggtcaggatt
    47761 tttgaaattc tggatatgtt gcgccaaatc gaccttgaga atgtcctcgt tgactacagt
    47821 cacattgtca aaatcacgca aggtatctgc caaaatcggt accaaacgat ggtcgatctc
    47881 aaaggccata acttctgctg cacgctcagc caaaaactcg gtcaaggcac caatacctgg
    47941 cccgatttca atgacattga cctggtcatc aatctcagcc gtatccacga ttttttgtag
    48001 gatattggta tccgtcaaga aattttgccc aaaggacttt ttaaaggtaa aaccgtgacg
    48061 ctccagcact gccttggtca cgctataatc tgcaattctc atctattctc ttttctatta
    48121 tctgttacta ctattgtaac acattcccct tgtattttgg gggtgaaaat cataccaaaa
    48181 aactatcttt cctcatgaag aaagatagtc ttggaaatct ttaaaagtgg ccttagcgct
    48241 tctctcgaaa catacgttca tactcttcga aaatctcttc aaacc
//
LOCUS       DAVBGY010000010        46765 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVBGY010000010 DAVBGY010000000
VERSION     DAVBGY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46765
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.010"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..89
                     /locus_tag="VQ620_000578"
     CDS             <1..89
                     /locus_tag="VQ620_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2763256.1"
                     /translation="STTYRNHRKRFGLRINLSAGIINHELGF"
     gene            complement(252..503)
                     /locus_tag="VQ620_000579"
     CDS             complement(252..503)
                     /locus_tag="VQ620_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763257.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYIENVYLDSLSVKQKFKFIFDFGDEWRFEC"
     gene            complement(793..2673)
                     /locus_tag="VQ620_000580"
     CDS             complement(793..2673)
                     /locus_tag="VQ620_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET2763258.1"
                     /translation="MVRFTGLSSKQAQAIEVLKNHISLPDVEVAVTQSDQASISIKGE
                     GGHYQLTYRKPHQLYRSLSLLVTALAEADKVEIEEQAAYEDLAYMADCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSSLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRHDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRIQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(2667..3536)
                     /locus_tag="VQ620_000581"
     CDS             complement(2667..3536)
                     /locus_tag="VQ620_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2763259.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILNKTSIPTPESLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQIPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGQTDWDGRKIYQEAAAGNAICQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEENQW"
     gene            complement(3620..6265)
                     /locus_tag="VQ620_000582"
     CDS             complement(3620..6265)
                     /locus_tag="VQ620_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HET2763260.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKDHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VAATGSLLSHSVLSIPQICPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTELIKKEEI"
     gene            complement(6356..7636)
                     /locus_tag="VQ620_000583"
     CDS             complement(6356..7636)
                     /locus_tag="VQ620_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HET2763261.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSPEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGLMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            7807..9891
                     /locus_tag="VQ620_000584"
     CDS             7807..9891
                     /locus_tag="VQ620_000584"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HET2763262.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHATDELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALLNLPQEDFDSCKASAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(9931..11610)
                     /locus_tag="VQ620_000585"
     CDS             complement(9931..11610)
                     /locus_tag="VQ620_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET2763263.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNLDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFDCRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKIRVVITESQALPLLTKISLYKT
                     PRLSQTVAVQGLAFAEKSLAVAKGENAYFTVKRRECSGPLEAKISIQPGTGVHGVAYQ
                     DEIQVLAFQAGETEKSLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            12365..13594
                     /gene="arcA"
                     /locus_tag="VQ620_000586"
     CDS             12365..13594
                     /gene="arcA"
                     /locus_tag="VQ620_000586"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HET2763264.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESEYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELILSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            13652..14668
                     /gene="argF"
                     /locus_tag="VQ620_000587"
     CDS             13652..14668
                     /gene="argF"
                     /locus_tag="VQ620_000587"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET2763265.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            14833..15780
                     /gene="arcC"
                     /locus_tag="VQ620_000588"
     CDS             14833..15780
                     /gene="arcC"
                     /locus_tag="VQ620_000588"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET2763266.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVEADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            15991..17502
                     /locus_tag="VQ620_000589"
     CDS             15991..17502
                     /locus_tag="VQ620_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HET2763267.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            17524..18855
                     /locus_tag="VQ620_000590"
     CDS             17524..18855
                     /locus_tag="VQ620_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET2763268.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNDLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            18951..19078
                     /locus_tag="VQ620_000591"
                     /pseudo
     CDS             18951..19078
                     /locus_tag="VQ620_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
     gene            complement(19261..19551)
                     /locus_tag="VQ620_000592"
     CDS             complement(19261..19551)
                     /locus_tag="VQ620_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763269.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(19539..19790)
                     /locus_tag="VQ620_000593"
     CDS             complement(19539..19790)
                     /locus_tag="VQ620_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HET2763270.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGDQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            complement(20189..21073)
                     /locus_tag="VQ620_000594"
     CDS             complement(20189..21073)
                     /locus_tag="VQ620_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET2763271.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(21218..22369)
                     /locus_tag="VQ620_000595"
     CDS             complement(21218..22369)
                     /locus_tag="VQ620_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET2763272.1"
                     /translation="MAIFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHRCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPQALTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIEHENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIVEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(22515..24281)
                     /locus_tag="VQ620_000596"
     CDS             complement(22515..24281)
                     /locus_tag="VQ620_000596"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HET2763273.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGCAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDSGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(24338..27454)
                     /locus_tag="VQ620_000597"
     CDS             complement(24338..27454)
                     /locus_tag="VQ620_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET2763274.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEAYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            complement(27464..29758)
                     /locus_tag="VQ620_000598"
     CDS             complement(27464..29758)
                     /locus_tag="VQ620_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET2763275.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPVNLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            complement(29817..30617)
                     /locus_tag="VQ620_000599"
     CDS             complement(29817..30617)
                     /locus_tag="VQ620_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2763276.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            complement(30614..31387)
                     /locus_tag="VQ620_000600"
     CDS             complement(30614..31387)
                     /locus_tag="VQ620_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2763277.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            complement(31412..31882)
                     /locus_tag="VQ620_000601"
     CDS             complement(31412..31882)
                     /locus_tag="VQ620_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2763278.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            complement(31873..32304)
                     /locus_tag="VQ620_000602"
     CDS             complement(31873..32304)
                     /locus_tag="VQ620_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2763279.1"
                     /translation="MFNRGILVASHGDFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISNYDSKINLEEIVHNAQNSLFNVKQQLNVEEEENLCL"
     gene            complement(32297..32737)
                     /locus_tag="VQ620_000603"
     CDS             complement(32297..32737)
                     /locus_tag="VQ620_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HET2763280.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            complement(32749..33387)
                     /locus_tag="VQ620_000604"
     CDS             complement(32749..33387)
                     /locus_tag="VQ620_000604"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HET2763281.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            complement(33502..34905)
                     /locus_tag="VQ620_000605"
     CDS             complement(33502..34905)
                     /locus_tag="VQ620_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HET2763282.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            35081..35854
                     /locus_tag="VQ620_000606"
     CDS             35081..35854
                     /locus_tag="VQ620_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2763283.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPDQKLLDAVELFDGEWVSDIQ"
     gene            complement(36974..38479)
                     /gene="adcA"
                     /locus_tag="VQ620_000607"
     CDS             complement(36974..38479)
                     /gene="adcA"
                     /locus_tag="VQ620_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HET2763284.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(38489..39295)
                     /locus_tag="VQ620_000608"
     CDS             complement(38489..39295)
                     /locus_tag="VQ620_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET2763285.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGRSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            complement(39288..39992)
                     /locus_tag="VQ620_000609"
     CDS             complement(39288..39992)
                     /locus_tag="VQ620_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET2763286.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNTHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            complement(39992..40432)
                     /gene="adcR"
                     /locus_tag="VQ620_000610"
     CDS             complement(39992..40432)
                     /gene="adcR"
                     /locus_tag="VQ620_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HET2763287.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            complement(40587..41855)
                     /gene="dltD"
                     /locus_tag="VQ620_000611"
     CDS             complement(40587..41855)
                     /gene="dltD"
                     /locus_tag="VQ620_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HET2763288.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            complement(41848..42087)
                     /gene="dltC"
                     /locus_tag="VQ620_000612"
     CDS             complement(41848..42087)
                     /gene="dltC"
                     /locus_tag="VQ620_000612"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HET2763289.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            complement(42101..43345)
                     /gene="dltB"
                     /locus_tag="VQ620_000613"
     CDS             complement(42101..43345)
                     /gene="dltB"
                     /locus_tag="VQ620_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HET2763290.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            complement(43342..44892)
                     /gene="dltA"
                     /locus_tag="VQ620_000614"
     CDS             complement(43342..44892)
                     /gene="dltA"
                     /locus_tag="VQ620_000614"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HET2763291.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFVQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMIMDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGVTHFYFDG
                     EELTVKTAQKLRKRFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            complement(44913..45044)
                     /locus_tag="VQ620_000615"
     CDS             complement(44913..45044)
                     /locus_tag="VQ620_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HET2763292.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            complement(45358..45630)
                     /locus_tag="VQ620_000616"
     CDS             complement(45358..45630)
                     /locus_tag="VQ620_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HET2763293.1"
                     /translation="MFLLFSALVGMLSQLKEVPESVFLQETVEENHLVNVYSVLEVIS
                     TLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK"
     gene            complement(45686..46379)
                     /locus_tag="VQ620_000617"
                     /pseudo
     CDS             complement(45686..46379)
                     /locus_tag="VQ620_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT        13938 a        10201 c         8447 g        14179 t
ORIGIN      
        1 tttcaacaac ctatcgaaat catcgtaaac gcttcggatt acgaataaat ttgagtgctg
       61 gtattatcaa tcatgaacta ggattctagt tttgcaggaa gtctattgaa atactaagcc
      121 tacacagact gatttcaatt ttaccattct tcatagtcaa aaccatcata atctggatat
      181 tgttctggca acgttccaac cgaacgcacg agataagctt cttcgtcctc tgtctcgatt
      241 tctctcagca cttagcattc aaaacgccat tcatcaccga agtcgaaaat aaacttaaat
      301 ttttgtttga cacttaggct atccagatag acattttcta tgtaacgttc ttcaacatcg
      361 tcactaacaa agctaagaaa gtaagaatct gcatgactcc actcaacatt atccatgaaa
      421 aatgcatgag catgatcatt tgcaaaatcg aaactccata aaatcacatc tgctagttcc
      481 gctaacgttt ttgtttttgg aatctatata tgtcggtagc aacctttttt aatttgacgg
      541 aaatgatcca agcttctaca gatgctggct tttcagcttc tatttcttgt tttactaatt
      601 ttaactgtag catttgaaac cagataattc ttctgtttca cgcgaaacac cctgcgccaa
      661 gcaaggtgtt cttgttttta tactcaatga aaatcaaaga gcaaactagg aagctagctg
      721 caggctgctc aaaacactgt tttgaggttg tagatggaag ctgacgtggt ttgaagagat
      781 tttcgaagag tattaagtcg tataaatcgt cgaagctgtc gcaatggtat gccactggtt
      841 agctgtagtt gctgcgaaat ccttgtctgc gtagaagtca gtaaatggta ggatttcaac
      901 ttccagctcg tcgatgcggt caagctggcc agccagataa acctcgatac ggctttctgc
      961 tcgtttgatg cgttgcaaga gtccgcccat acggatgtca actgtatcca aaccaaagac
     1021 cttgttttct ttcagccatt ggtgactaaa gagggcatgg aagtcttcaa tttggattct
     1081 aagttctggt aattcttgtc tggcgatttg ttgtaaactt tctttatcat ccgcttggta
     1141 ggcctgacga atgcgtcgtc ccacatctac tttgctactt aaaatagcat tcaactgggc
     1201 ctgagtttca aagagataag catagtttcc agctttttct ttaatgttag caagcgtctc
     1261 agcagcctga gcgaagtgcg gtttgtcctg ttcaggtgtc atgtgtcggt caagtatcgg
     1321 acaaagaaca tcctgataaa aaacatagcg gttgggattg ataccgctga gattgcctgg
     1381 taggtctggt aagaggttgg caaggtcaat ctgcataaaa tcttcaaccg ttagaccagt
     1441 attggtcttg aagtgcgcag acaaaccatc taggtcatgg cgatagctga gttctgccca
     1501 gatttgcaag cttggtagga tagagaactg ggcagtttca ccaccattgt ctccccaacc
     1561 cgttacgatg acttctttaa tctgattggc acggcaggct ttattagcct cgatagccac
     1621 tagactgcta aaatggttgt gaggtgtaaa gccaatccac ttccaagctc cccctgcaaa
     1681 tgcaaggtca tggctaatct tgtgatgatt gcggaaatta cggttgtatt tttcctcgct
     1741 atcctgataa taatcccagt aaaccagagt cacacggtct ttgagacggt ctaggtagac
     1801 acgagtttcc tctggaattt ccacatcacg gtcgtactgg ccatccgctg acatgagttt
     1861 gaagaacata tcactccaca tctggcagtg gaaaccatat ttgtcagcaa tatccagcac
     1921 gcgctccaag tgttggcaca tgaggagact acgatccaca acaccgttca aaatcaggta
     1981 gcgtcccaaa ccaaccaagt gggcttcgtc catcccgata ttgaccttgc gagtcttcag
     2041 tttagacaac gtggcaaaca tgccatcaat caggtcataa accttttctt cgccaatgag
     2101 aagaatgtcc tctacatcac ggagctcctg cacttccttg acaccccatt tgacaaaggc
     2161 cgacaagtgg gccaaggtct ggatgcatgg tacaaaggtc atgtcaaact gttgagcata
     2221 ggcttcgatt tcctgcaact cctctgctga ataagctcca cggaaatagc caaagtaagg
     2281 ctgtccttca atctggtaag tgtcttccat gtagagctca aaggttgagt agcccatgag
     2341 agccaagacc tctatcatct gcttggcaga agccacattc agcaccgcat ttcgagaaca
     2401 gtctgccatg taggctaaat cttcataagc tgcctgttcc tcaatctcta ctttatcagc
     2461 ttctgcaaga gctgttacca acagggacaa ggaacgataa agttggtgag gcttgcggta
     2521 ggtcaattga tagtggccac cctcaccctt gatagagata gaggcttggt cagactgagt
     2581 aacagccact tcaacatctg gcagagaaat gtgatttttt aatacttcaa tagcttgcgc
     2641 ttgtttggaa ctaagtcctg taaatcttac cattggtttt cctcctgtag ccagttgaca
     2701 agggcaccgt agagattagc atctgcatga taggtgcagg cttggataac gggtgcgacc
     2761 gtatattctt cgtaagtatc gacaaagtct tcaacagcct tcttgacacc ttgaataaaa
     2821 tctggatttt gactgataga gcctcccaga ctgatgacat ctggatcaat cagatactgg
     2881 atattgagca agccttgcgc caaattgcgg ttcatgcgct caatggcttc ttggcaaatg
     2941 gcattcccag ctgcagcttc ttggtaaatc ttgcgaccgt cccaatcagt ctgaccagat
     3001 ttttcaatca cgtagcgaac catattccca gttgacgcta gttgcgacca gttgttgagc
     3061 ttttcagcag gggcaagggt tgtcatatag ccaaattctc cacccaagcc gtggcgacct
     3121 cggtgcagtc tgccattgat aatcatagct ccaccaatcc ctgtcccaat cacgacacag
     3181 gctgcatttt caagttctgg atgagccagt aattcactga gtccaacgca gttggcatca
     3241 ttttccagat ggacaggaat ctgataagag ctaagcgcct cataccaaga aaagccatgg
     3301 atgtagggca ccgcactgaa gccatcaatc acacctgtct cttgattgac cgcacctgga
     3361 acactcatag caatcccact gtaatcctgc tctgacaagc gctggtctag ccaagctagt
     3421 aaatcctcca agctttcagg cgttggaatg cttgtcttat tcagtatttt tccatcagga
     3481 gtcagactgg caaacttaat tccagtccct ccgatatcaa tcgttgcaat ggtcattgtt
     3541 tttaccataa aaaggggcga atcagcagtt agttcttact ctttcgcccc tcctttctct
     3601 ttatgtttac tttttgaaat tagatttctt cttttttaat caattctgta cgaatttctt
     3661 gtggagccaa tagtcctgaa tgaactgggt atggtcgttc aagtaggtca agaatagttt
     3721 gttggtgttc tgatatgcgc acattttctt gactcatatt gtagtaacgg aggacatatc
     3781 cttcttcatt ttccgccact ttaaaggctg ttggacaaat ttgcggtatg ctgagaacag
     3841 aatggctcaa gaggctacca gtcgcagcca cgcttccttc ctgtctagca agctgaaggc
     3901 tggtaaacgg tgtctgcaag gccttagctc gacgataagc tgagaagcgt tcttgggctt
     3961 ggtggcactc aagcgcaaac tcgacttcaa attcacgcaa gcattgtgct tctggcgttg
     4021 ggaagtagcc ccagtcacct agctcacctg atgcacgcaa aatggtcacg gcaatggtgt
     4081 tatccccaag gatttcgtat tcattcaatc ccttattgga tacagtcacc cctttttcat
     4141 cgtcatacag actgacaaaa gcttgttggt gttgaggatt ttcagggttt tcccatgaag
     4201 cagctggttt gtttggtcgt gtcaccacct catagatact ttcagaatca ttgcttggac
     4261 gcgtgttatg agtcttgacc aagagacgga tacggtgatc cttggcagtg ttagtaaagc
     4321 gagtcttgaa gcggatttgt ggattgtcaa cgaagacagt caactcagtt tccagaggaa
     4381 tgtttgtcaa ttcttctgac cgtccagcct cacgcttcat aaactcgatg ataccttgtt
     4441 gctcttcttc tagcttttca tccgcactga caggcacggt caattcatgt ttgagcaaga
     4501 ttttagcata gcgatctgtg ttttccaaga cctcgtggtc cttaagctct gcaaagattg
     4561 gctctgttcc ttttggttgg aaatagatat actcatttcc gatgtcaccc cggtcttcga
     4621 agcggataaa atcttcatag gcttcgtgag ttgtcttatc atagactgtg atgttgtcat
     4681 ccacactcac cgttacgaat ggtgtatcaa tcactccgtt ttggtaaata ccctcacggt
     4741 gttcttgttc tccttccagc aattggaagg ttgtccaaga aagtggcgct agatgaactg
     4801 gaatggtcac gcgcacttgt cgagcaatac gagcttggcg gaacttgtct tttggtaaat
     4861 cgtattcaaa attagctccg aggtcttcga ttgtagcctc tacaggacga ccatccaagt
     4921 cctccacacg gtaacttggc aagataagag cagccatctt tttgtagcct tctgttgggt
     4981 gcaattcctt gaaatcacaa gtcgccacat caatcactgt gctgacagta tcgaccttat
     5041 catgcaagcc tgtgttaatg acagtaaaga gatagtcact ttgagcctta tccgtagcaa
     5101 ttttaccctt ccactcgttg agcaaattac ttttaacaaa gtttcctact tggttgacct
     5161 tggcaaaacg cgtttccatc tcgcggtgaa cttcgtccac gctacagcca cagatactat
     5221 catgtggcgc attctgcaaa agtgttttcc aagcataggt caactggtcc ttgtggttgt
     5281 gtccaccagt gataatagtc aagggttcta ccacttgctc taggaggttg ctattttctt
     5341 ggaaggcttg ttttaggtaa atgcgggatg aagaagtgtt ggcaagtgtg taccagccat
     5401 ctgtttcctg actggtcaac tcacctgtaa cagttgataa gtgttcagga agcgcacctt
     5461 ctacagcttg aacatattca tcaaaagaac tatgaacaaa gattacatcc gggaagagtt
     5521 catttgccac acgaatggct tcgctcagat ttttctggac aggctggtgg tcacagccgt
     5581 tcatcatcaa ccattggttg gtcgaagcgt aggcacgcac atctgacaat ttttgtttcc
     5641 agaaggtcaa ggcctcatct ttgtcaactg ggatttcatt cccattactg taccagttgg
     5701 caaagagaat acctaaaaca cgactaccat ccacaccctg ccagtacatt tctgaaaact
     5761 gggatgtaaa ctgctcatct tcaaggactt ggttgtcaaa tccaatcggc ttcacaccac
     5821 gaccaaaggc cgccacgtga atgcctgatt tttgaagaat ttgaggcgct tgtcccatat
     5881 ttccaaaggt atctggaaag tagccaatct gggttgattt accccatttg gcagcttctt
     5941 gttgaccaat caaggtattg cggacattgg cttcactgga gatcaagtag tcatcctgca
     6001 agatgtaaaa gggaccaatt ttaagtttgc cctcgtcaat gtagcgttgg accttgtcgc
     6061 gattttcagg gcgaatttgt aagtagtcat caaggacaat agtttgtcca tccaagtgga
     6121 aactcttgaa ctcagggtca ttttcaaaga gatcaaagag attgtcaaac aattccacca
     6181 actgcatacg atggctttca aaaggcaagt accactcacg gtcccagtga ctatgtgaga
     6241 taatatgtac aacaacattt tccatgaagt aaaacctcat tctaaattta aattttaacg
     6301 ttttaaatgt aaacttgtga ttctgataag aatcggttga gctaaagcga gccccttagc
     6361 gaatatccaa gtaatccaag accaactcac agaacatcat gttagcccag gagaaccatt
     6421 cacgagagta gagagttggg tcatccacgt ggaagctttc atgcataaga cctgtaccgc
     6481 catcgcaagc aaccagctga tccagcaaga attttttctc tgccttatct cttgttgtca
     6541 agccttggat agaaagggcg attggccaga tatagcgata gaaggtatga gaactgccga
     6601 gaccgctagc gtattctcct tggtagaaat atggattttc agggctcaga atggtacggc
     6661 gagtagcttg atacacttca tcatcgaccg aacagtagcc cagatagggc gcagctagta
     6721 gacttggtac gtttggatca tccataatgc tggcatttcc taggccatcc acttcaaagg
     6781 cataaatctt ttcaccctta ctgttggtgg tgtaagcgta gttttcgatt ccttcttgga
     6841 tttcatcctg aagacgcttg gcatcagcaa taacactctg gctatcagct aggtttaatg
     6901 ctgcgaagat ttcttgcaca taacccaaga ctactacagc aaacatattt gacggaatca
     6961 agtaactata ctggcaacag tcatcactcg gacgaaaagc tgaccaagtc atacctgtca
     7021 ctgcaaaatc aggtccaaag ccatcattta ccaaggtgtc ttccttgcgg tccgtatctc
     7081 ggacaaaacg ataaggagag ttcttatgat cttgttctac cgtccacaga tgaagaattt
     7141 ccttagtcgc tgcgacaaaa gtctcatcaa actgactagt ctcgccagtt tctttccaga
     7201 ggagataagc caactgcaaa ggatagcaaa gtgaatccac ctcatacttg cgctcccaga
     7261 tccagccgtt aaggtctgtg tggtcagtct cgtggtgccc tttccagttc tcctcaatgt
     7321 tgaaggagtt agcataggga tccttgagta ccaaggtcat ctgacgtttg accaaacctg
     7381 caatggtctg acgcaggagg gcatctcttt tagctacatg aaggtagggt ctgagttggg
     7441 ctgtcgaatc tcgaagccac atggcaggaa tatccccagt caagacaaaa gttgaaccat
     7501 cttctaagat ttcaaccgta ttgtccaagg tatcggtgta gcaacgctcg aaaacatcca
     7561 cccactctgg atggtcctta gcccgctctg ctacttcatc tagccattct ctaacaattt
     7621 ctttcgaata aatcatcgtg caattccctc tttcaaacaa gtttcatgtt cagtatatag
     7681 cttttgagac agaaaataca tacacaaatg atagtcattt ttctacaaaa tagttatttt
     7741 tgaaactcct tttctaaagg ccctcttttt ctgatataat gtagaaaaac agctaaagga
     7801 aagactatga aaccactact tgaaaccatc gatacccgct ttggaactac caacaagcat
     7861 gccttttctc ggggaaatac cctgccatac acaggcgtac cttttgggat gaattacttt
     7921 gtgccccaga ccagtgacca ggatggatct tggttttttg atccgcatct gcctatcttt
     7981 caggggattc gattaactca ccagcccagt ccttggattg gggactactc ttggctcctt
     8041 ctgacacctg tcaccagtca actgggggga gacagcctct ttcaccgcca gtcttcttat
     8101 gatatagata aagcttgctt ccagcctcac tatttgaagc tcttttctct gcgttatcag
     8161 attgaaaccc agctcacacc gacttgctac ggtgcttcta ttcgtttgaa gcaaaagcaa
     8221 ggcaaagccc tctccctcta tcttcacgca acagatgaac tgacagtaga gcaagtagat
     8281 aagcgaactc ttgccctgcg acaagaaggt aaaactgaaa ccaacaaaaa ttcgctaacg
     8341 atgttcactg ccctgcaaat gaacacggat attcttgcta tcagccaaga agctggggac
     8401 tggcgaattg acttagcaag tagtcaaacc gagatgcaac tagccacttc tttcatctct
     8461 ccttctcaag cactgcttaa tctacctcaa gaagattttg atagctgtaa agcaagtgcc
     8521 caagcggatt gggaaaatct cctccatcgt tttgacatta tagagacagg agaggctgac
     8581 cgaaccttct ttgaccactg cctctacaga ctcttcctct tcccacagac tttttatgag
     8641 gttgatgagt cagggcaagc catccacatg gatctggcta ctggtactgt caagcctggt
     8701 gtcctcttta gcaacaatgg tttctgggat accttccgca ccaccttccc cctctttgcc
     8761 cttatcatac cggagcacta tcaacgcttt ttagaaggtt tcctcaatag ctaccgcgat
     8821 actggtttcc ttccaaaatg gctggctcca gatgaacggg gtatgatgcc aggtacactt
     8881 ttagacggta ttatcgcaga tagcgcctgc aaggacatgg cccctgacct agaaggagaa
     8941 ctcttccaag ccatgctcga aacagccagc aaagccgacc ctctcggaat caatggccgc
     9001 cacggactag cccaatacca agaactaggt tacctctcta ccgaccacca cgaaagtgtt
     9061 agccatactc tagactatgc ctatagtgac ttttgtatcg ccagctgtgc caaaaaacta
     9121 gagaacatag aaatcgctga aacctacaag gctgcttcac aaaattaccg ccagctattt
     9181 gacgctgaga caggttacat gcgagcacga gacaatcaag gaaactttca ccctgacttc
     9241 tctccttata gttgggggcg agactacgct gaatgctctg ccattcaagc tactttaggc
     9301 gttctccacg acatccctgg cttaatccaa ctgatgggtg gaaaagaaac ctttagcaac
     9361 tatcttttga aagcctgtca agatgctccc ctctttgaaa caacaggcta tggttacgaa
     9421 attcacgaaa tgagcgagat ggctactgct ccttttggac aaatagccat ttccaaccag
     9481 cctagtttcc acattcctta tctcttccgc tacagcgatt accctgacta cactgccctt
     9541 cttatcaaga cgctccgtca gaaagctttt cacccaagtt gggaagccta tcctggagat
     9601 gaagacaatg gtagtctctc tgcttggtac atctggtcag ctctcggatt ttatccaacc
     9661 tgtccaggaa aaccaagcta tgacctcgga atccctctct ttgaccatct acgagtctac
     9721 ctcgctaaag aagataaatg gctggatatc catactaaac aaaaccacaa ccattttaac
     9781 tttgtcaaag aatgccgact ggacaaaaca ctcgtatcaa ctattcaaca ccaagacctc
     9841 ttaaaagctg aacaactaac tttcaccctc agctggttac caagtcacta ataaccaaaa
     9901 gaacctttgc atcgcgcaaa ggttcttctt ttatgaaact tggacttgaa gttgatccac
     9961 aagctgacca tccaccgtta ggttcagata gaaatccaag gttttatctc ctgcgaaata
    10021 caaggttggt agcgtcagac ttttttcagt ctcaccagct tgaaacgcaa ggacttgaat
    10081 ctcatcctga taggcgacac catggacacc tgtccccggt tgaatcgaaa tcttagcttc
    10141 taaaggacca ctacattctc tgcgcttaac tgtaaaatag gcattttctc cctttgccac
    10201 agctaggctt ttttctgcaa atgctagccc ctgaacagct actgtttgtg ataacctcgg
    10261 agttttatag agtgaaatct tggtcaacaa aggcaaagcc tgtgattctg taatgactac
    10321 acgtatcttc tgtgcctcta ctagcggacc tcgcaagagg cgcttatgac caactgtgaa
    10381 gcccctgcca aactcctgcc agacaccatc cacctctact tgcacatgaa aagcagcgat
    10441 tcgttgccct agcttcaaat cttctcttaa ctcaattaca tcaaaagttt taggagaacc
    10501 taaatcgagt tctaactgga tgggcaagtc tgcatcgctt gcccaagagc tggtctcaag
    10561 gccgtctgtc aaatggcgac aatcaaagtc tgcggaaaga gctggaccag atacctcagc
    10621 tcccagagcc aaatcttctt tatagagctc attgcgatag gtcgcaaatt cataaagtcg
    10681 ttcaatatcc tttgcatcaa agagcccagc ttgattcggc ggaatattaa gcaagagtgg
    10741 agttcctcgc cctactgagt gaaagtagat ttcgaccaac tcctcgagag acttaggatc
    10801 ctgatcctca tggtagaacc agcctggacg gatggaaaca tctgcctctc cgattgaaaa
    10861 aatcgtgccc gagggatccc cgtgctgaag atagttcagc tctgcttctg ttcctagttt
    10921 atcaagattc accttttgcc acagtggatc acctgcatac cctcgttcat tgccaatcca
    10981 gcggatactg gtgccttctg ttgaaaaaat caagcaatcg ccctgcaggt cacgaatggt
    11041 ttcaaaccat ttttcaaatt cataattaac cttttgcgcg ccctctcctc tggcaccatc
    11101 catccaaacc tcagcgaact taccagcatt cccatagtta ggatttgata agatttcctt
    11161 caactgagcc agataatagg cattgtagtc cgcttctcgg tccacatgat agaggggact
    11221 atgggcatcc cacggtgaca ggtagacccc catatccata tcaaactctg tggcagcttg
    11281 ggatacttca aggagcaagt cgccctttcc tctcctccaa ggactgacct taaccgaata
    11341 atctgtgtga gctgtcggat aaaggacaaa gccatcatgg tgcttgacca ccaaaatcaa
    11401 ctttttgaag cccgtttcct tgagcacacg aacccactca cgcgcatcca actgactcgg
    11461 gttaaagcgc tcaggatcct cctgtccagt cccccattct tggtcataaa aggtattagg
    11521 accgaagtgg ataaaagctg ctagttcatc tcccagataa gctagctgag tctgacttgg
    11581 taacggtcca tgcggtttga ttttcttcat cgttatatat ctccttcttt agcaacttct
    11641 cttgttcaca aggttatcca caggttgtgg ataataaacc atgatctaac agccagctct
    11701 ttccaactcc aactggtagg ctcaggattt ttctcctcaa tattccctct tcaaacagct
    11761 ctgtcatatg gttctgctaa cacctctcag aatgctactc tccataatta ttctcaggct
    11821 tctttcaggt cattgtgtga agaccattca ctctccagac ttttccacag attgtgaata
    11881 agtgattatt ctaaactatc acctcgtcga ctataacaaa aaataaaaag agggtcaata
    11941 agataattat ctcttcctaa atactcagaa aagattctat aatttgtaca attgaacaag
    12001 aaatgcttct ccaaaatcta tcttcaaaag aatgttgcat ctttttatcc tttcacacaa
    12061 acatttctct ttatacagat ccaagtgata accagatgat aattggacac tttttggtca
    12121 tttgtatatt ttttgaaaaa ttttttaaaa aaattttata tcctttttat agaagtttat
    12181 gcatttgtat ctatgtattc tatcctatgt tatgtataat aacataaaat tgtgctaaac
    12241 atcataattt atattatttt ccgagaaatt atagccattg acattaccta ggaattgcgt
    12301 tataacatac atgtaagcgg tacccaaaat ggtgccaagt caaaattttt aaggaggaaa
    12361 atacatgtct tcacatccaa ttcaggtctt ctcagaaatt gggaaactga aaaaagttat
    12421 gttgcaccgt ccaggcaagg agttagaaaa cttgttgccg gactatcttg aaaggcttct
    12481 ttttgatgat attcctttct tggaagatgc tcaaaaagaa catgatgcat ttgcccaagc
    12541 tcttcgcgat gaaggaattg aggttctcta cctagaacaa ctcgctgctg aatcattgac
    12601 ctctccagaa atccgcgatc aatttatcga ggaatactta gacgaagcca acatccgtga
    12661 tcgtcaaacc aaggttgcta ttcgtgaatt gcttcacggc atcaaggaca accaagaatt
    12721 ggttgaaaaa acaatggctg ggattcaaaa agttgaattg ccagaaattc ctgacgaagc
    12781 taaagatcta actgacttag ttgaatcaga gtatccattt gcaattgacc cgatgccaaa
    12841 cctctatttc actcgcgacc catttgcaac aattggaaac gccgtatcgc ttaaccacat
    12901 gtttgcagat actcgtaacc gtgaaacact ctacggtaag tatatcttca aataccaccc
    12961 aatctatggc ggaaaagtgg atttggtcta caaccgtgaa gaagatacgc gtatcgaagg
    13021 tggagacgag ctaattcttt ctaaagacgt ccttgcagta ggtatctctc aacgtacaga
    13081 cgcagcttct atcgaaaaac ttttggtcaa catcttcaag aaaaatgttg gcttcaagaa
    13141 agttttggcc tttgaatttg ctaacaaccg taaattcatg cacttggata ctgtcttcac
    13201 tatggtagac tatgacaagt tcactattca cccagaaatc gaaggcgacc ttcacgttta
    13261 ctcagttact tacgaaaacg aaaaacttaa aatcgttgaa gagaaaggtg acttagctga
    13321 acttcttgct caaaaccttg gtgtagaaaa agttcatttg attcgttgcg gtggtggcaa
    13381 tatcgtagca gctgcgcgtg aacaatggaa cgacggttct aacactttga ccatcgcacc
    13441 tggtgtggta gttgtttatg accgcaatac cgtgaccaat aagattttgg aagaatacgg
    13501 gcttcgcttg attaagattc gcggaagtga attggttcgg ggccgtggtg gacctcgttg
    13561 tatgtctatg ccatttgaac gtgaagaagt gtaatcgctg ttcggcattc gtcaatagaa
    13621 aatgtaaaaa atagaaagag gaaataataa aatgacaaat tcagtattcc aaggacgcag
    13681 cttcttagca gaaaaagact ttacccgtgc agagttagaa taccttattg gtctttcagc
    13741 tcacttgaaa gatttgaaaa aacgcaatat tcaacaccac taccttgctg gcaagaatat
    13801 cgctctccta tttgaaaaaa catctactcg tactcgtgca gcctttacaa ctgcggctat
    13861 cgaccttggt gctcacccag aatacctcgg agcaaatgat attcagttgg gtaaaaaaga
    13921 atctactgaa gatactgcta aagtattggg acgtatgttt gacgggattg aattccgcgg
    13981 attcagccaa cgtatggttg aagaattggc agaattctca ggcgttccag tatggaacgg
    14041 tctaactgac gaatggcacc caactcaaat gctcgctgac tacttgactg ttcaagaaaa
    14101 cttcggtcgc ttggaaggct tgacattggt atactgtggt gatggacgta acaacgttgc
    14161 caacagcttg ctcgtaacag gtgctatcct tggtgtcaat gttcacatct tctcaccaaa
    14221 agaactcttc ccagaaaaag aaatcgttga attggcagaa ggatttgcta aagaaagtgg
    14281 cgcacatgtt ctcatcactg aagatgctga tgaagcagtt aaagatgcag acgttcttta
    14341 cacagacgtt tgggtatcaa tgggtgaaga agacaaattc gcagaacgtg tagctcttct
    14401 taaaccttac caagtcaata tggacttagt taaaaaagca ggcaatgaaa acttgatctt
    14461 cctacactgc ttgccagcat tccacgatac tcacactgtt tatggtaaag acgttgctga
    14521 aaaatttggt gtagaagaaa tggaagtaac agacgaagtc ttccgcagca agtacgctcg
    14581 ccacttcgat caagcagaaa accgtatgca cactatcaaa gctgttatgg ctgcgacact
    14641 tggtaacctt tatattccta aagtataatt ttagataata aaccgtctac caacagctat
    14701 gagggctgcg actaatagct ttagtccggt cctcttttat ataatggtaa tctattattt
    14761 cttataaaat atgtgaaaaa tcattaaatt gaaatctaaa cgcattctat tgagtgtgat
    14821 aaaggagaat ttatggcaaa tcgtaaaatt gtagtagctt tgggaggaaa tgcgattctt
    14881 tcttctgacc catcagcaaa ggctcaacaa gaagctttag ttgaaacagc taagcatctt
    14941 gtaaaattga ttaaaaatgg agatgatttg attatcactc acggtaatgg acctcaagtt
    15001 gggaatctct tgctccaaca tttggcatca gactctgaaa agaaccctgc cttcccactc
    15061 gactcacttg tcgctatgac agaaggtagc atcggtttct ggttgaaaaa tgctttgcaa
    15121 aatgctctct tggatgaagg catcgaaaaa aatgttgcct ctgttgtaac gcaagttgtc
    15181 gtagataaaa atgatccagc ttttgttaac ttgagtaaac caatcggtcc tttctattca
    15241 gaagaagaag caaaagcaga agccgaaaag agcggagcga ctttcaagga agatgctggc
    15301 cgtggctggc gtaaggtcgt tgcctcacca aaacctgttg acatcaaaga aattgaaacc
    15361 atccgtactc ttttaaataa tggtcaagtc gtcgtagctg caggtggtgg cggtattccc
    15421 gtcgtcaaag aaaacaatgg acatttgact ggtgtcgaag cggttattga taaagacttc
    15481 gcttcccaac gtttggcaga attggttgag gcagacctct tcatcgtttt gacaggtgta
    15541 gattatgtat ttgttaacta caacaagcca aaccaggaaa aattggaaca tgtgaatgtt
    15601 gcccagctgg aagaatatat caaacaagat cagtttgcac caggtagcat gcttccaaaa
    15661 gtagaagcag ctatcgcttt tgtcaatggt cgtccagaag gaaaagcagt tattacttcc
    15721 cttgaaaatc taggcgcctt gattgaatct gaaagcggaa caattattga aaaaggataa
    15781 gttgttttac taataagatg tattctattt ctagtatctt tatatcaaat tagaaattat
    15841 tcttgaaaac atgtacaata tttcaaaaaa tattagtttt ttagacttta atatggtaaa
    15901 acaaatataa atagaaagcg ttttcttaaa tgtttattta agaaagtagt tggtttttta
    15961 cactttgtta gacatcagga ggaaaaacaa atgagtgaaa aagctaaaaa agggtttaag
    16021 atgccttcat cttacaccgt attattgata atcattgcta ttatggcagt gctaacttgg
    16081 tttatccctg cgggggcctt tatagaaggt atttacgagg ctcagcctca aaatccacaa
    16141 gggatttggg atgtcctcat ggcaccgatt cgggctatgc taggtactca tccagaggaa
    16201 ggttcgctca ttaaagaaac gagcgcagcg attgatgtag ccttcttcat ccttatggtt
    16261 ggtggtttcc ttggcattgt caacaaaact ggtgctcttg acgtaggaat tgcctctatc
    16321 gtgaagaagt ataagggccg cgaaaaaatg ttaattttgg tactgatgcc tttgtttgcc
    16381 ctcggtggta caacttatgg tatgggggaa gaaacaatgg ccttctatcc actccttgtg
    16441 ccagttatga tggccgttgg ttttgatagc ctgactggtg ttgcaattat tttgctcggt
    16501 tctcaaatcg gctgtttggc atctactctg aatccatttg cgacaggtat tgcttcagcg
    16561 actgcgggag ttggtacagg ggacggtatc gtacttcgtc tgatcttctg ggttaccttg
    16621 actgctctta gtacttggtt tgtttaccgt tatgcggata agattcaaaa agatccgact
    16681 aagtcactgg tttatagtac tcgcaaagaa gatttgaaac actttaacgt agaagaatct
    16741 tcatctgtag aatctacact tagcagcaaa caaaaatcag ttctcttctt atttgtgttg
    16801 acattcatct tgatggtatt gagcttcatt ccatggacag accttggcgt taccattttt
    16861 gatgacttta atgcttggtt gactggtctt ccagttattg gtaatattgt cggttcatct
    16921 acttctgcac taggtacttg gtacttccca gaaggcgcaa tgctctttgc ctttatgggt
    16981 atcctgattg gtgttattta tggtcttaaa gaagataaga ttatctcttc cttcatgaat
    17041 ggtgctgctg acttgctcag tgttgccttg atcgtagcga ttgctcgtgg tattcaagtt
    17101 atcatgaacg acggtatgat taccgataca atcctcaact ggggtaaaga aggcttgagc
    17161 ggtctatctt cacaagtctt tatcgttgta acttatatct tctatctacc tatgtcattc
    17221 ttgatcccat cttcatctgg tcttgccagc gcaactatgg gtatcatggc tccacttgga
    17281 gaatttgtaa atgtccgtcc tagcttgatt atcactgctt accaatctgc ttcaggtgtc
    17341 ttaaacttga ttgcaccaac atctggtatt gtgatgggag ctcttgcact tggacgtatc
    17401 aacattggta cttggtggaa attcatgggc aaactcgtag tcgctattat tgtagtgacc
    17461 atcgcccttc ttctccttgg aaccttcctt ccattcctat aaaatagtga gtgaggtgat
    17521 tccatgaaaa tagatataac aaatcaagtt aaagatgaat ttcttatatc attaaaaacc
    17581 ttgatttcct atccttcagt actcaatgaa ggagaaaatg gaacaccttt tggacaagca
    17641 atccaagatg tcctagaaaa aactttagag atttgtcgag acataggttt cactacctat
    17701 cttgacccta aaggttatta cggatatgca gaaatcggtc agggagcaga gcttctggcc
    17761 attctctgtc atttggatgt tgttccatca ggtgatgaag cagattggca gacaccgcca
    17821 tttgaagcaa ctatcaaaga cggctgggta ttcggacgtg gtgtccaaga tgataaaggc
    17881 ccttcgctcg cagctctcta tgcagtaaaa agcttactgg accaaggtat tcagttcaaa
    17941 aagcgcgtac gctttatctt tggtaccgat gaggaaaccc tctggcgctg catggcacgc
    18001 tacaatacca tcgaagaaca ggccagtatg ggctttgcac ctgactcatc ttttcctctg
    18061 acctatgctg aaaaagggct tctacaggtc aaacttcatg gccctggatc ggatcaacta
    18121 gagcttgaag taggaggcgc ctttaacgtt gtaccagaca aggccaacta ccaaggtccc
    18181 ctctatgaac aggtttgtaa cgatctcaaa gaagctggtt atgattacca atccactgaa
    18241 caaaccgtaa cggttctcgg agtgccaaag catgctaagg atgctagtca aggtatcaat
    18301 gctgtcatcc gactagctac cattcttgct cctctccaag aacaccctgc tctcagtttt
    18361 cttgcaacac aagcaggtca agacggcaca ggaagacaaa tctttggtga tatagcagat
    18421 gaaccttctg gtcacctatc ctttaatgtc gcaggtctca tgatcaatca tgaacgttct
    18481 gaaatccgta ttgacattcg gactcctgtc ttagctgaca aggaagaact agtagagttg
    18541 cttacaagat gtgcacaaaa ctaccaactc cgctacgaag agtttgacta tctagcgcct
    18601 ctatacgtcg caaaagacag taaactcgtt agcacactga tgcaaatcta ccaagaaaag
    18661 actggcgata acagtcctgc tatttcatcc ggtggtgcca cttttgctcg caccatgcca
    18721 aattgtgtag ccttcggcgc cttattccca ggagcgaagc agacagaaca tcaggcaaat
    18781 gaatgtgccg ttctagaaga tttgtaccgt gctatggata tttatgccga agccgtctat
    18841 cgacttgcaa cttaatcagg caactgtttc taccaaaaaa aaatcgaccg attaatgaac
    18901 tgcaccccaa aagttagaca gaataaatct aacttttggg gtgttttatt atgaaattga
    18961 gttatgaaga taaagttcag atctatgaac taagaaagca aggacaaagc ttcaaacagc
    19021 tttcaaaaag atttgtgtgg atatttctgg tctaaagtca tctgaatctt tgagatgagc
    19081 tttataaatc gcttttttca gtttttgcac tggtgtttcg ataaactcaa actttttagc
    19141 cgtggtattg cctgatttta tagtatattg aaactagaat agtacacctc tacttctaaa
    19201 acatttttag aaatcgattt gactgtcctg aacgatttgt cctgttctta tttcatttta
    19261 ctatatttga gccacttcgt ctttaacggc tttattcata agctcttgta atttttcttt
    19321 actatcaatt acttctgatt ttccgttgta atttattgta ataggtttta acttacctaa
    19381 tttctcgaca cgctcattaa tttgatcttt tttgaaggct gcatatgttt ttcctaagat
    19441 tttttcgaaa atatatttat cagatagcgg tttgccttct tcttcagctt ggtttttgta
    19501 ttgatttgaa acataaggaa caaatccttc atagtaacct aatgctccca taagttcaaa
    19561 agcttgtttt ctaattcaaa ccattgcaac tcagatttca gcttttcaga taaatcctgc
    19621 tcatccaaat aatgacttga aattagtgct gaactcgttt ctgtatcctg tacaggctga
    19681 tcacccatac cagcaaaaaa taaactcgtt cctagcaaga ccgaacaagc tcctattgca
    19741 tatttcctca aagaaaaacg ctgctttctc tcaaattgaa attctttcat cccatctccc
    19801 atcattcatt attactgtat attttgtata tcagaaatag tttgtattca caaatctttc
    19861 tagttattcc cttatcattc ctaattaagg gaggtaacat acaataattt ttagttaaat
    19921 gtatatctat gttttttgtt tttcttaata aacgcaatac aaaaagagcc tgttgccaag
    19981 ctctttgtac tcaatgaaaa tcaaatagca aactaggaaa ctagccacag gttgctcaaa
    20041 acaccgtttt gaggtagtag ataagactga cgaagtcagc tcaaaacact gttttgaggt
    20101 tgcagataga actgacaaag tcagtaacat ctatacggca aggcgacgct gacgtggttt
    20161 gaagagattt tcgaagagta ttagtctatt atttcttctc agcgcgaagg gctgacaaga
    20221 tttgtgttcg gatatcatcc acaccatttg gcgtatttgg taaaaagatg gtttgatttc
    20281 ctttagaggc aaaggtattc aaggtatcca aatactggtt ggtcaagagg atagacatga
    20341 tttgttcttc tgtcatgcca acattggctt ccttgagttc ggtgatagac tctgccaatc
    20401 catccacaat cgccttacgt tgttgggcaa tccccacacc atgaaggcgg tctttttctg
    20461 cttctgcttc agctgcagtg acaattttaa tcttgtcagc ttccgccaat tcttgtgctg
    20521 cgacccgctt acgttgcgcc gcattgattt cattcatgga ttgcttaact tctgcatccg
    20581 gttcgacttt ggtaatcaag gttttcacga taatgtagcc gtaagtggtc atttcttctg
    20641 ctacttggtg ttgaacttca agggcaatct catctttttt ctcaaacaat tcatccaagg
    20701 ttaattttgg aacagaagag cgaagagcat cttcgatata agatttaatc tgagattctg
    20761 gacgtatgag tttatagtaa gcatctgtca cgctctgctc gttgacacgg tactgagtcg
    20821 ctacattcat cataacgaac acattgtcct tggtcttagt ctcaaccaca atatcacttt
    20881 gcaacaagcg caactgaatc cgtgctgcaa tcgagtcaat cccaaaaggc aagcgaatat
    20941 gaataccgct attagcaacc ttttggtatt tcccaaagcg ttcaataatc gccaccgact
    21001 gctgacgaac cacataaact gtactcagtg tgactatcac caataggagc acacaaacaa
    21061 tcagaaaaat catgaaaaat attgccataa tggaacctcc acaagtattt ttctagtatt
    21121 atagcacatt taaagaaggc tgtgccgttt ttactgcgat ttttcctgaa atgtcaataa
    21181 ttagaggtga attgtcctat tgtcgtccaa tctcttgcta aaataactct ttataaaagg
    21241 caatcgtttc ttctaaggtt ggcataaatg gatttcctgg tgcgcaggca tcaatcaagg
    21301 cattcttaga aaggtattca aagtcgaaat ctttttcttc aataccaagt tcagtcagtt
    21361 tcttaggaat acctactgtc tcagaaagct tctcaatctc aacaatcgca taatcggcac
    21421 attcttgatc tgatttacct tctacatgaa gtcccaaggc tttggcaaca ttgcggaaag
    21481 cttctggtac acgtttagca ttttcatgtt ctataactgg tagcaacatg gcacagcaaa
    21541 caccatgtgg caaattatat actgcaccga gttggtgagc cattgagtga acataaccaa
    21601 gaccagcatt attaaagctc ataccaccaa ggaagatggc attgaccata ccttcacgcg
    21661 cttcaatatc atgtccattt tctacagcac gaggaagata ttccttgata agctcaatag
    21721 caccaataga cagtttcttg gttacatcat aagcaccagg tgttaccaaa gcttcaacag
    21781 cgtgagtcag agcatccata ccagtagcag cagtcaaggc ttgaggtttg gaaagcatga
    21841 gttcagggtc attgacagaa atcaaggcaa ggctattctt gtcaaccatt accatcttaa
    21901 ccttgcgttc ttcatcagta atcacatagt taatagtgat ttctgctgat gtacctgccg
    21961 ttgtattgat agccacaacc ggcaagcctt ttttagcaga cttatgaagc ccttcataat
    22021 cctgtggttt tccaccattt gtagccatga tagaaataca actagctgca tcctgtggag
    22081 aacccccgcc aagactgatg ataaaatcac atctatgttc ttgcaaagca gctaccccat
    22141 ctgtgacatt tttacaagta gggtttggct ctacatcgct aaagatgaca tattcgattc
    22201 cttctgtatc cagtggtttt aaagtcttag gtaaaatatc actgccttcg atgtacttat
    22261 ctgtcaccaa aagtgccttt ttatagccaa gttccttgat ataaggaccc acttcattta
    22321 caacaccttt tccaataagg ttgactgccg gaacataaaa tatagccata taattttcct
    22381 cctgcgcctc cgcgctatct atttaacatc attatattac tttttgtatt cgttttcaat
    22441 tatttgtgaa ttgtttaaca aatcttttta aaaggatagg agggacgtaa gttcacctcc
    22501 tatataaaca agttttattt atgtagtggt cccaacaact gacatgcacg atagtctgct
    22561 gattctagat cttctgttcc aaataaggac caatttttag gtctaaagat atcttcctca
    22621 ggtacattat gcatattgac aggaattctc aacatagaag ccaaggtaat caagtctgct
    22681 ccaatgtgtc catatgttat ggctccgtga ttagctcccc aattattcat gacgtcatag
    22741 acagacttga aagcaccttt tcctgtcaaa cgtggagcaa accaagtagt tggccatcct
    22801 gaatctgtac gattatctaa agtatggtga acatcttcag gaagttcaag tgtgtaacct
    22861 tctgcaattt gtagcactgg accaacccct tttagaagat tgagacgtac cattgttact
    22921 ggcatatccc ccttcgtcaa gaaacgagtt gagaatcctc ctccacggaa gtactcgcgg
    22981 tttgctggtg ggaagtctgt attttcaagc atagcctgta cttcactttc ttccaactcc
    23041 cagaatggtt tcataacagg tttgccatct cgagtagctt gacctgtacc atccaatgta
    23101 caagaaccag agttgattag atgtaagaag ccagctgcag cacaaccctc taaagtatgt
    23161 cccgttacac gtttaacagc ctctggactc caataagtgc gcacatcagc aaagatttgt
    23221 ggagtatttg ttaatagata attaaagagc atagacacac catttagtga atcattctct
    23281 gtcgcaaata caaatggttt tcgaatacca ttccagtcaa actgagtatt gaggaaagtt
    23341 tccataaaat ctccatttgg aaaatggtct gtccactgac gttgaccttg gaaaccagct
    23401 actaaagcat ggtgaccaac cgcttcttcc tcaaaaccaa gttcagcaag ccttgggtta
    23461 ccaaccatta agtcacgtcc aatcatgaac atcttaataa caaattccca ttgtttttct
    23521 ttctcttctt tagaaagaat caaatcctca cgatttcggt caaccccttc tttgatatgt
    23581 tcttttaccc aaaccatagc acgttcgaac tcttctgggt cataaatacc acggtccata
    23641 cggcgcgtga actccgtcat atctaccgat tcatttcgca ttcctaagta ttcttggaag
    23701 aaatctggat ttacaataga accgccaatc cccatcgaaa cactacccat tgataggtaa
    23761 gcagtgtctc tcatcaagcc agttgcaaga gctgcacgcg cataacgtaa aagtttttct
    23821 ttgacatctt ctggaatatc tgtatcatta gcttcctgaa catctctacc ataaatccca
    23881 aaggctggaa tccctttttg agcatgtgaa gctagtacag ctgcaagata gacagctcct
    23941 gggcgttctg tcccattaaa tccccaaata gcatgaggaa tatctggaga catatccata
    24001 gtttcactac cataacacca gcatggtgta actgtaattg ttgcgcaaac atttgatttt
    24061 ttaaacaact catgggaagc tgcagcctct ggaacacgac caatggtaga tggagaaatc
    24121 acacattcca caggttcccc atctggatat ttcaatgtgc ttgaaatcaa atctgccaca
    24181 cttttagcca tgttcattgt ttgtacttca agtgattcgc gtacaccttg acgacgacca
    24241 tcaatagtcg gacgaatccc aatacgtgga tgttgaatca tactattttc ctcttatttt
    24301 tctgtttctt tcatactacc gatgataatt tttaaaatta cttataagta taaacctcta
    24361 cctctgccaa acttaaagga attcctgcgt ttctcaaaga aattctaacc ttttttccct
    24421 tcagcccttt taagtctatg gatagattat tgctttctaa actgtcaata tgttgtttaa
    24481 aaacttcatg attatttgaa tcatatataa taatatcaaa atttgataat ctctgaattt
    24541 cagcatctgt tctattataa ataattaact tttctaattc ttcggtttgt gctaaatcta
    24601 tctcccacca tgaaggagaa tctcctttgg tatgagtcac tgaatgatga ccataatctc
    24661 cgtttttatt tccatcaact gctagacgag aaaaaccatt gtaatccgtt gaactctgtc
    24721 gagtttcttt ggttaatgca atattagaaa gtttcttcgg agttctcttt gaaccatagg
    24781 cttcaacttc cgctaaactt aacggaatag ctgctgattt taattctatt ttgactagct
    24841 tagcccctac agaaggtact tttaaagata acaaaccatc aactacttta tcaaaatgtc
    24901 ttctaaaaac ttcttctcct gatgaagata gaaaaataac atcaaaatta gataaacgct
    24961 gaggttcggc atctgttcta ttataaatat caactttaga aaccgtaaac tcttctccca
    25021 gatcgacctg ccaccaagcg ttagaatctg ccttagtatg agtcaccgaa tgatgtccgt
    25081 aatctccatc tttattacca tcaactgcta aagcagctac accctcataa ttggttgaac
    25141 tttgagtagc taccttattt gtagagacta ctttatcttc tgttatttta tctatatcct
    25201 cttcagattg cttaccatct gattctctaa aaacctctac ttctgctaaa ctcaaaggca
    25261 ctccactcgt tagtaattta actttaatat accttgctcc tttttctttg aaatctagac
    25321 taacagattc acccgacaaa ttattaacat gtttcttagc aacttcgttt ctattattgt
    25381 catatagaat cacatcaaaa ttagatagac gttgagtctc agcatctgtg cgattataaa
    25441 ttttaacaag cccaacttta tccatttttt tcaaatcgac ttcccaccaa gagggattat
    25501 ctgccctagt gtgtgttacc gaaccagagt taaaattacc atttctgtta ccatccacag
    25561 ctctattagg gtctccattg taatctacag aactttgtgt tgttggtttt gcatatgcta
    25621 tattcaaatt accatcatta aatttatctg gtgttgggaa agggactggg cctgtccctt
    25681 cagtatttat agacatctct accattccat tatgattaac cgtaatggta taaacatggg
    25741 tattctcatc ccaattttct gtataagtat aatgattttt atcgccactt atatttatct
    25801 gaggtttatg accagtatgc ccttttaatg taagtgttgt tgtcctaaag tcattatcta
    25861 caggattgat ggaataattt ttgcttatcc agttcgctaa ctctaattct tctttcttcc
    25921 aactttttga tgcatcaaaa tttcctgata atgcccacat agctgtctta tctgtcctgt
    25981 aattgttcaa taaaatatgt aaattatttg gattttcttt aacaacagcg taagtatgtg
    26041 gcgtcaaatc taacgataac gactttgtat tattagtata cataggcaac attacttgct
    26101 gatttttatt gatattagca ttactattat aaatatacca ggaattacct acacgctgag
    26161 catacccatc tccttcataa agttttggat aaagcgagtt taaatagttg actttactag
    26221 acaattcctc actattttta gtcaaaattt ttgcattagg gaatatagat gaaatctttt
    26281 ccttatcaat tttctcatgt attacaggaa gaatatgata tctcccatta ttatataaag
    26341 gcattgtttc atcattcgaa taaagtcctt gataaaatcc gtttaatgaa ctaatcctac
    26401 cttctccatt ccaaaataca gcttttgttc tatttacaac ttcttcctta cttggagctg
    26461 gattttgtat agcatgtcta aagaaaggaa taataccttt agtaaatgct ggagttggta
    26521 catcatttgt cataaatgta tacgcggcac attcaaaatt ataaactgtg cctcccccag
    26581 tatatacatt catcatttcc atagcaatca ttgattctgg ttccgatgca taggatctca
    26641 tatccctagc tcttccagtt tcaaatgtgt ttgtataatg tttttcccac catttccatg
    26701 tatctgttga tgagccccag ttatcacata agccactcaa ccaaaatcca ctaacgatag
    26761 aatctgtacc cgcatcatct cttattggcg tatttttagt tgccaacacc aaatttttat
    26821 gatatttttg actcgcttca aagaatgtcg gatcattcat aatagtttcc cagaaccatt
    26881 tttcatgatc atgccagata aaatgcgctc catatttggc acaaacttcc aaatatttag
    26941 cactatgcgg agctaactgg ttattgtaaa tccaataatt ctcaatattt aaaacacctt
    27001 ttaacacact atacttttgg aattgttcat ctaaccactc tggaggaact gtattacgct
    27061 ctccagccga cataataacc aagaaaactg gaatgtttag actttgagct tcctccaaca
    27121 tcttgacata ccattctctc aattctttcg tatctcgtgg aatacaactt gttggtttac
    27181 agacttttgc aggatgaagt tcaatcgctg catatggttt tacatcttca ggaatagctt
    27241 cccatgctcc cttcaatgtg ttcccccacc ataatccgtt accattatca ttgccataca
    27301 acggagaaag caacaatggc gattcattat taatcgtcgt tctcatttga acacgattat
    27361 cagccttaac tcctgctaac aatccaaaac ataacatccc aatacataca aacagtattg
    27421 tgattaattt tctttttatt ttttctttat tcatgactta tacctaaaaa tttaactcaa
    27481 taattttttc tgaattaaat accaattcaa tattttgtac atcagtattt ttgccatata
    27541 ttcttactct tactttttga tctttgtttc ctccttccaa ttttaggaat gttatatccc
    27601 catttttcca agcaaacgat accttatatc ctcctcttac tctgaaacct ttcacttctc
    27661 cttctgacca agcagaaggt aaagctggaa ttagtgataa ccaattatga tggctctgta
    27721 ctaataattc acaaattcca ctcaccaaac ctaaattacc atcaatttga aatggtggat
    27781 ggtcaagaaa taaattgcca agagtcgcat tatttaacaa accattaatc tggttataag
    27841 caggttcacc ttgatatagt ctcgcaaaaa aatgaatcag ccatgcagca ctccaacctg
    27901 tttgtgtact agcatgcaaa ccacttacta accaattatt aatcgcttgc tccctctcct
    27961 gtgaagataa aaagttagcg tttgataatc tcctattgat agtgatttta gctgcttctg
    28021 ctaattccgg agttttatga atatcaatct cattataagg ataaagccca aatagaggtg
    28081 aaatgtgtct atgcccaggc tctacttctt cataatcttc taaccattct tggatttgcc
    28141 cattactacc tatttttgtt ttaggtagtt tcttttttaa ctccttcaca cgactaataa
    28201 aatccgaatt gtctcctaat tgttttgcaa tgccaatgca tgaatcacaa aaatatctta
    28261 gaatttgatt atcaattgta gatgatagac aagcatttcc ttcaatacca ttttttaagc
    28321 gatatttatt ttccggtgag acacttggac ctgtcatcaa gtagccatcc acctcaaata
    28381 aataatcttc aaagaaaaga aatgcttctt ttatcatttc aaaatgttcc gtaagaatac
    28441 gctcatcttg gaaatataaa tagtgttccc aaatatgagt acataaccat ggaatagtta
    28501 atacccaaat tgcagccccc atggcatgag attggggagc cgtatcgcca aaaccatccg
    28561 tattatgatg tgctgtaaaa cctctagctc catacatttt cttagcggtt agtcttcccg
    28621 gttctctcat tctttcgagc atatcaaata atggatattc tacttctggt aaatcacatg
    28681 gacctaccat ccaataattc atttgagtat taatattaat cgtatattta gaaccccaaa
    28741 ttggatttaa ttcatcacac catattcctt gaagattgac aggtaaacca ttcggttgac
    28801 tagacgatat taacagatat cttccataat gaaataacaa gttagtcaag tagttactat
    28861 actttttagt gttttcaaga agtagattcg ttggaatgct aagacaatct ttactatagt
    28921 ctagtttaaa atcaactcta ttaaattgct cctgatattt ttttacatgt tcatcttttt
    28981 ctgtaaagta atcaatacta ctaaattctc cctgaagaga agaaatatct atatttcccc
    29041 aataatccgt cattgatttg agataaagaa atacctctgt agcattccga ataactattg
    29101 tctctcccaa tacacttact tcaccatccg taactttaga atgacatact actttaaact
    29161 gaacaccttt tctacctcca gcagaggccg acattaaaat tgtacttgaa tccagtttag
    29221 atacttcgtc attaaaccgt ttatttctac ccaaattaat gtttaaattt aatgtgtttt
    29281 gaactgatga cactatacgg caacataaaa tattcttatt aaaactcgta aaatattctc
    29341 tttttatttg taaattacaa ctattaggct caaacacaac attagaaata gctgtatcta
    29401 aatctagctc tctttcatac aatgaaagag cacaagactg aatatctata tgctcaatgt
    29461 aaagttcccc aagtaattca tagtggcttt gatctcttgg ggtagcaaac attgttaact
    29521 ttatcaattc ttcggctttc tgaatttctc catctaaaag atattcccga atttttttaa
    29581 gatgcaatag tgagtctgga ttatttctat ctgactttcc tctataccaa atagtctcat
    29641 cgtttagttg aatacattct tttgtagctg aaccataaat cataccacct aaatgaccgt
    29701 tcccaatcgg caaggcttca ttccaatttg aggcagcttt cttataccat aatttcactc
    29761 tttacctctc ttccaaaatg gaggtgatat cccaaatacc acctccacac tttctactac
    29821 ttcaaaattc ctaaaacaac taggataact ccaatagcca taacactaaa gattagtaca
    29881 accggattct ttccttttct aattaattta cacattaata aagtcaaagc catagggata
    29941 aatcctggaa ttaattttgt aatcatttct tgaacagaaa taacaagttc cccctgctta
    30001 aattctaatc caaaattaat accaaccatt gatggaatca aaccacccac tagtactagc
    30061 cctaatgctg tcgccatact cgtaacgcga ttcaatgttc cttttgtatt attttcttgg
    30121 ataagactag aacccttagt atacccatat ttcaaaccga aatatttaac aggaatatta
    30181 ataatattaa acaatattaa ggcgataaag atacctaaag caccgccgtc tttagaataa
    30241 gacgcaccaa tactaaaaca gataggaact agtgtcagcc agaacaaact atctcctaga
    30301 ccagccagtg gccccatcaa gccaacttta ataccagtaa ttgaagctgc accttcattt
    30361 ccttcttgtt cttccatagc ggaagtaact ccaagaataa atggtgccgc tgtttgatga
    30421 gtattgaaaa actcaaggtg acgtttcatt gcaggagaag ctgaatcttt gtctgggtat
    30481 agttttttca atactggaag aattgtatat agaaaaccta ggttctgcat tctctcatag
    30541 ttccatgaag aaccatacat aaaacttcgt ttgaaaactt ttttaagttc aactttagtt
    30601 actttattat tcatcataga tcaagatcct cctcaacatc actacttgaa gttgtttctt
    30661 gtggtttact tccaatcata tcgtagaagt atgctaccgc aataccaact agtgagatcc
    30721 caatggttga cattcctcca tacacagaac aaatgaatcc aatcaacaag aatacccaca
    30781 tatttttctt gagcatcata tttaaaagca taccaaaacc aattgaaggt agtaaagcac
    30841 ctgcagagtt aagtccctgc ataacgattg gtggaacctt agcaaacaag tctgccacat
    30901 attcccctcc aagcataata gctaggaaaa ttggtacaat tttaaataac gtgattaaaa
    30961 ccaaacttga ataatgataa aaaccgatct ttgaataatc tccgttttta gcatcattat
    31021 cagctttttt cacaaaataa acatctaaag tttttgcaag aacatccaac tgttgggtag
    31081 caactgcaat tggaactgct atagcgatac cagcagtttc tccttgacca gataaaatac
    31141 caaatgcagt accaataatc gcacctgaaa tagtatctgg tggagtgtaa cctccaatac
    31201 ctgttacacc gagccaagtt aattcaagag ctgaaccaat aagaacacct tgggtgaaat
    31261 ctcctaagac taagccaacc attgcacttg taactaacgg acggtgaatc ataaataacg
    31321 gtccattata gtcaatagtg gcaataatcc caactaatgt caccagtaat gcttgtgtga
    31381 atagcatata ctgtacctcc ttaaattatt tttataaaaa tttttccaac attgtaactt
    31441 tatcattagg aaccatttga atttcaactc tcgttccctt atcaactatt ttcttaaaat
    31501 aatatttgtc ttcttcacct agagctacac ctcccgttac cttttcattt tcctccgttt
    31561 tactcatctg tcctacattg aggtactcca attttaaatt tccttcaata gaatcataca
    31621 catcttttgg atttgtatat atcagcattg ttctcttttt tattggcaca gagtttaaaa
    31681 cttccacaaa ccgttttaca ctaaagaaaa caatttttaa acctactggt gcagaaatct
    31741 ttaaaataga ttgtcgtgtt ttatcttctg agatgcgatc attaacaatg ataacttgct
    31801 caatatcata cttttttagc cacgtagtga caacttgacc atgtaccaga cggtcatcaa
    31861 tacgaacaaa ctctatagac ataaattttc ttcctcctct acgttaagtt gttgtttaac
    31921 attaaacaaa ctattttgag cattgtgaac aatttcttct aaattgattt ttgaatcata
    31981 atttgatatt aattccacta ggagaggaat attaaaccct gttacaatat caactgaatc
    32041 caaatttaaa aaccgtgaca aagccacatt attaggactt cctccaatca agtcagtcaa
    32101 aacgataacc tcttgatttg agtctaacag ttcatccact tgatttttaa aataatgctc
    32161 aaactctaca atattctctc caggcatcaa acctaatgtc ctaactctat catttgttgt
    32221 ctcaccaaca aacatttctg cggtcatgag agctccgcta gcaaaatcac catgactggc
    32281 aactaaaatt cctctattaa acattttctc tttcaacaac tactcctttc ttaaggataa
    32341 tattagcata aagtgaagtt tctcctgtag caacaatagc ataagctttc ttactacgtt
    32401 cataaaagtc ttctcttcta agataagtaa tcgttttaag atctgtacca tgaccttcaa
    32461 tcatctgtct ataggtaccc catatcttag gaatatcatc acccgaaaca acgttcataa
    32521 actgaattga actatcgacg taactatcta atggcattaa atacagaatg gaatctaata
    32581 attctggaat atttacgcca tcacaacgaa ttagcttatt tgcacatgag gcagaaggat
    32641 aattcgcgtc agctaatact atttcatctc catgtcccat ttccattaaa gtcttcaata
    32701 aatctggaga aatatttttc ggtatatgtt ttaacattaa tatcctccct attttctctg
    32761 accgtatgtt ttaaattttt ctgccatcaa ttccatctcc tcatcaggaa gaactactgg
    32821 ctctccaaaa ttcttagcta aacaataaat ttttgcacaa tattcaactt cttcaacaat
    32881 attaaatgca ttcaataaat tttgtgcacc tgctaaaatt ccatgattcg ctagtaaaac
    32941 tgctctacga ccttccattg ctttagctgc attcacagcc aattctttcg tgccatatgt
    33001 tgcatactca gctacccgaa catctttccc tgccactgca atcatataat gactcgctgg
    33061 aagtggttct ctgagacaag ctaatactgt tgcataagtt gtatgagcat ggataattgc
    33121 atcgatatca tcacgagttt gatattgaat caaatgcata taccattcgc tagatggcaa
    33181 gcgttctccc tctacaacat ttccattaat atccatcact acaatatcgg attctttgat
    33241 ttcaaagaaa tcaatacccg acggggtaat tgccatcaat tgtttttcac gatcgaaaac
    33301 gctgagattc ccacctgttc ctttcgtcaa atctgtttct actagcttct taccatattt
    33361 aattaattct tgttttacat ctgacatcct tgtcctcctc atatcaaaac actaaatatt
    33421 tttcaattca ccaattatcc ccccttcctg cacaaaagct aagaaggggg agggaggcaa
    33481 atttacaaac tctcaatctt tttaattctt ttgggagtaa taactctcta attgtagaaa
    33541 ctcctcaata acatggtgag cctcttccat cccttttaat tgtcccatag ctatgagctg
    33601 cacaacaata ttccccacag ctgtagcctc agtcaaacct gtaagaacct ctttaccagt
    33661 tctatcagca atcatttggt taaaataact ggctctagca cctcctccaa tcacatatat
    33721 cctcttataa accttatgag ttagttcttc taataactct atcgctttcc tatacgtttc
    33781 agctaggctt tcataaacaa tcttaaatag ttgtccatct gtccactctc tagtttcatg
    33841 atgataagct agatattctg tcaaagtctt gtgcatatca gattctgttg caaattcagt
    33901 tgattcagta tcaatcagag gaagattttc tttttctttc tccaccattg tcctaatatc
    33961 atcaaaagaa taggctttcc ctcttcgttc aaatgaacgt cttagttcct ctatgatcca
    34021 caaccctgta caattcttca gaaatgtaat cactccatct ttaccgactt catttgtaaa
    34081 tccataactg aaggattcgg tagtaagaat cggtgaagta agttccactc caaccaaaga
    34141 ccaagtacct gatgaaataa ataaactacc ttctgtctta ggtactgaga caatcgcact
    34201 tgctgtatca tgactacaaa cattcacaac aggaatatcg cctaaaccat actccttttt
    34261 tatccttcca agaacatttc cctctgaaac aatttcagga agtaaagatg aatccaattc
    34321 aaatagtttt aagatattct gattccaatt ttgactccta ggatcaaata attgagttgt
    34381 tgaagcaatg cttttttctg tagcaaactt acctgtcaag agataattaa acaaatctgg
    34441 cattaaaaga atcttattgg tcttatagaa agagtcagga gattcttgac gtgccttaaa
    34501 gagttgaaac aaggtattta tctccataat ctgatttcct gtctctgaat acagtttttc
    34561 taattcagtc atttcagata tttcctttaa cactcccttt gttctttcat cacgataatg
    34621 aacaggttgt gataacagct taccttcatt atcaatcagt ccaaaatcaa ctccccatgt
    34681 gtcaataccg atagataaaa tcttgtaact agtattagcc aagcggatac tttcaagaat
    34741 tttagctagt agaaagtcaa tatcccaaga taaatgccct tttactctaa taggtagatt
    34801 agaaaagcga tttatttctt ccattactag tttattttca gaaaggtaac caacgattgc
    34861 tcttccagaa gtcgcaccta aatctattgc taaaaccgct tccatttttc ttctctcttc
    34921 gtccttgatt aactttatta taatccccac gttttatttt ttcaatatgt gaacgcttac
    34981 ttgaataatg ttcaaatgtt aacccaatat tcataaaaat aattcaaatt cctccaattt
    35041 gtgctataat atagttgaaa tgataaatta ggaggagctt atgataaaag atgaacgtgt
    35101 acttgaattg attgaaatta ttaaaaagaa aaaaagaatt gccgtaaaag agctggcaga
    35161 aatcactttc tccagcacaa gtaccttacg tcgtgattta attttcttag aaaatcaagg
    35221 tcttatcaaa agaaagcacg gatacgtgac cctgtcctct atgaacacaa ttgaactttc
    35281 tcatcaaata cgggaaggag aaagtactag gcaaaaaaga ctaatcgcta gtctcgctaa
    35341 agactttatt cggtctggta tgtgtatcta tctagattct agtactactg tctacgaact
    35401 ttgtccctat ctttctgaac ttgataattt gattattttt acaaatggtt tacatactgc
    35461 acaaacccta tctgaaactg ttaaagatag ctccaaaatc tttatcacat ctggcgaggt
    35521 caaacatcaa tcctgttccg tggtcaacta tgataaggaa aattctttat tagatcattt
    35581 taatatcgat ttagcatttt gttcagcaag aggtattgat gaccaatatg tttatgaagc
    35641 ttctctcagc caagctattt caaaaaagaa tattattgac aaagcccatg aaaccatctt
    35701 actgattgat agttctaaat tttacaagac tggatttttt aaaattaatc ccctctccaa
    35761 atacacaacc tttatttctg acaccttgcc agaccaaaaa ttattagacg cagtcgaatt
    35821 atttgatgga gaatgggttt ctgatattca atgaaaatca aataggaagc tagtctcagc
    35881 ttgctcaaag tacagatttg aaaactgatc aagtcaagtc ccccatggac acggcaagac
    35941 gaagctgaca tgatgtgaag agattttcga agagtattcg ttctgtctgc tgactgattt
    36001 acaaatattc accacatgac attattttct tagcaattct aaaaacagag gctggacaaa
    36061 aactcaattt caggagaaaa tggagtaaac cttcccacaa taaaacgcat agtatcaagt
    36121 ttttgcacac ctgatattat gcgtttttct gattttaaag actttgtcgc ctagacccta
    36181 tatttcaaga attaagtgac cttgatgatc taaatatatt gtggtatact atttttgtca
    36241 tcggttaccg attacgttcc tatgggttcg tgagaggagg tgaaactaat gagccttatc
    36301 atagagctcg ctcttactat tatagcggat gttatagctg gaattatctt gtatttcgtc
    36361 tgcagatggc tagatagtaa ggagtagacc gagtgactag cctataaaca cccgttaaat
    36421 cgctaagata cgtcaaaaaa gcccttaact acggccatag ttaggggctt tggtgttcta
    36481 atgaacctta tcaattaact acattttagc atataatcta caatagttca agcattaagt
    36541 gaggttgatg atctaaattt agtgtggtat actatttttg tcatcggtta ccgattacgt
    36601 tcctatgggt tcgtgagagg aggtgaaact aatgagcctt ataccagagc ttgctcttac
    36661 tattatagca gatgttatag ctggaattat cttgtatttc gtctgcaaat ggctagatgg
    36721 taagaagtag accgactgac tagcctataa acacccgtta aatcgctaag atacgtcaaa
    36781 aaagccctta actatggcac tagttagggg ctttggtgtt ctaatgaacc ttatacacta
    36841 actacattct agcatataag cccagatatt tcaagagttt tatttattgt ttaaagttct
    36901 gaaaggtcta taatgaagtt agccatctag tatcaaaaaa ccgactagct cttatgaact
    36961 agtcgatttc tcatcaatgc gccaacattt cttgggcgat ttcttggcca gataggttat
    37021 ctgggtagta ggttggccag ttgtccattt cttcaaagag ggcttcttgg cttgtgcctc
    37081 caaagaagat atggaaatgt tctgccttaa ctggggcaac attgtggtca ctaaactgaa
    37141 catacttgaa ttgtccagcg tcagcatctg tggcttcaaa gaggaaacgc acgccacgat
    37201 tgcctttctt gtaagtcaaa attttcttac cgacatactt gtaagtgtat ttcttgcttt
    37261 gtccaccttg aacaaattcc atagtattat cagtaatgtt aatcttagtc acatctgtct
    37321 gatagccttt tgtatagtaa gccttgtact cagcctgggt catcttacca gtcaacttag
    37381 ccttgtagtc aaagacttgg tcaaacgtgc cgtcttcaag gaaaggataa actgattgcc
    37441 agttacctgc atagtcactc aaggtgcggt ccttgacagc tgcatcctcg aagtaaccat
    37501 tttggactgt cttggtatcc tctgcctttt caggttcgat tgctgggcct tcttggtctg
    37561 ttgtttgttt caaagccttg aggtttttct ccatcacgga aatgtagttt tctccagcct
    37621 tggtgtcctc ttctgtcaga ctttctaaag gattgaggac atcagttttg acacctgctt
    37681 cttttgaaag tgtgttagca agggcttgtg aggcattttc ttcaaaatag atataggcga
    37741 ttttattttt cttgacatac tctgtcaatt ctgccaagcg agcagctgat ggctctgcat
    37801 ctggagaaag tcctgagatt gcgacttgtt tgagtccata gtccaaggca agatagttaa
    37861 aggctgcgtg ttgagtcaca aagctctttt gttttgcttg agacaagcct tctgcgtaag
    37921 ccttatccaa ggcttgcaat ttttcgatat aggcagctgc attcttctca aaggtctctt
    37981 ttttatcagg ataatctgct gacaagctgt cgcggatgtg ctctactagt ttaatggcac
    38041 gaactggtga taaccaaaca tgggggtcaa actcatggtg atgaccttct tctccatggt
    38101 catggtctcc ctcttcttcc tcgccacctg gcaagagcaa catatcgcct gtcgccttga
    38161 tggttttcac ttttttctta tccaaggtat ctagcaattt aggtacccat gtttccatgt
    38221 tttcattttc ataaacgaag gtatctgcat cttggatttt ggcaactgcc ttggcagatg
    38281 gttcgtattc atgaggttct gtcccagcac cgattaggag ttctacatta gccgtatctc
    38341 ctgcgacttg cttggtaaat tcatagacag ggtaaaaggt tgtcacgata ttgagtttac
    38401 catctgcctg tttttgattg gaacaagcca ctaaaaacaa ggcacataga ctggctagta
    38461 ataagctaat ttttttcacg ttcgtctcct atttgataaa acgtcttact aaactgatta
    38521 gtataaagac agttacaaaa ataatggtaa tacttgcact tgcaggtgtt tctgcatagt
    38581 aggaaatgta aagtcctgct accattccca aaaagccaat cgcactggca agcagcataa
    38641 ccgatttaaa gtttttcccc agacgcaggg caatactagc tggcaagacc ataatggtcg
    38701 ataccagaag agctcctgct gcaggaatca taagggcaat agccacccct gtcaccatgt
    38761 taaaaagaat ggacatggta cgaactggca agccatccac aaaggccgta tcttcgtcaa
    38821 aagttaagat atacatagga cgaagaaaga gaaaggtcaa aatcaaaaca accgccgcaa
    38881 tgacaaagag ggaaatgacc tgttcttcac tgatagtcac gatcgaacca aagagatatt
    38941 ggtccaaact cattgaactc gagcttctac ccttgctcat gacaatcaga gaaacagcca
    39001 aacctgttga catgaggata gctgtcccga tttccataaa gctcttgtaa accgtacgga
    39061 gatactccag aaagaccgcc gcaatcaaga caatggcaat agtagaaaca gttggagaaa
    39121 tccccaaaac cagaccaaag gctacacctg aaagtgagac gtggctaagg gtatcactca
    39181 tcaaactctg acgacgcaag atgaggaagg ttcccaatac cggtgagaaa agactcatag
    39241 caataaccgc caaaaaggcg cgttgtataa agtcgtaaga taataaacta agcatggccc
    39301 acctcctgcc cattctcatg aacgttgaaa caacgccatg gcgagtcttg gttacggact
    39361 agatgaatat tgcgatccgc ataatcctta acttcttcag ggtcatgggt aatcatcaaa
    39421 acagccttgc catgatgatg ggcgctgtgg tgcatgagtt cgtaaaattc atttttactt
    39481 cctgcatcca tccccgttgt cggctcgtct aggataaaca catcagggtc agaagcaaac
    39541 atacgcgcaa ttaccgctcg ctgcttttgt cccccagata gagaccccaa gcgtttgtct
    39601 cgatgttccc acatgccaac tgagtccaga ctagccttga tatgctcctc atcatgagta
    39661 ttcaaacgac ggaaccagcc ttttctcgga tagcgacccg acttgacaaa ttcatagacc
    39721 gtacttggaa aaccagcatt aaaactggca atttgttgag gaagataggc tattctcaat
    39781 ttcttacctt gcgtatttgt ctttgaaata gccacctttc caatgcgtgg ttgcagaatt
    39841 ccaagactgg ccttgatgag cgtcgtctta gccgctccat tttccccagt caaggtaaca
    39901 aattccccac tatcaacaca ataattgata tgttcaagaa caggttcctt atcataatag
    39961 aaggacaaat cctctaccgt aatatatctc attatttgat ttctcctact aaagcagtca
    40021 aaaaccgctg aatcactttt tgttcatttg gagtaaactg agtcgccact tgttcatagg
    40081 ttaaaagtgt atgctcatgg tgatggtggt gctcctcagc gattggacga gccaagtcag
    40141 tcaactgata aaaaatcaca cgcgcatctt tagaatcttt agatgtttcc aacatccctt
    40201 ccttgaccaa agacttaatg gccttggtaa ctgccgcctg actgacattg agacgacggg
    40261 ccaattctga atttgttaaa gattcctctg acaagagcat aaggatatgc tcctgagtat
    40321 tggtcagggc cacctcgcta gtgcaatgac ctattaggat ttcatgctga ttttccgcct
    40381 gcaaaatcac ctcattcaaa aaagcattga tatcctttgc tagctgtctc atatctgact
    40441 cctttccttt tagacttctc ttttttaaga gaaaaatact attctttgac attttgttta
    40501 ccagttaatt atatcacaag caaaaaaaga gtcaagaaaa aacgtgaaaa ctagtttcat
    40561 tcttgaactc ttctatatta tattatctat tgaaattctt tgacatctcc atcataagtc
    40621 gcccaatctt tgctgaaaaa gcgctcattc agatggtaag tcggagctgg tgtgggattg
    40681 gataggaaag gatcaactgc cttgtcaaaa gccaaccaac ccaaccaacc aaggtgaatg
    40741 gtgtccttca taaagaaagg ctccccgccg tccttagaaa aatctgctat attggtaaaa
    40801 ccttgacttt ctaactggta gcgaatcttc tgcaccgttt gttggtacat atcctctcgt
    40861 agaccagcat agtccatcca ttttttatta acaggtggaa tgataaaaat cgggtttacc
    40921 ttagatttag aaaactgtgt taaaaccaac tgcaagtcat tatactctgg cgacttgaga
    40981 taggtaaagc ttttctgaga atcctttaat ttcttcaaat ccttcttgat ctgctcatta
    41041 tagaaataat tttccattcc catctcatta ttggaagtat ttttttcagc atctgctttg
    41101 acaacatctt ctattgcctg ataagaaaac tggtctggca agatttttaa atacttagct
    41161 acatgcttat cgtagttaac atagcctcta accgaaaact gaccaaaaaa ggaagcttgg
    41221 cgttcattaa aacgagccaa taattcaatc atttcattgt ctgctgtcga caattcttct
    41281 ttacttgcca acttctgaac caggtccttc atagctacgt ttgggaactg ttgcagtaag
    41341 cgagtcgctg catattgact agcctgatcc ccagattgat gtttcagaaa actagtcaac
    41401 tggtctccat taaaatactg ctggaaggct gctggatcat agccattttt actgaaccac
    41461 tgaggtgaga taacatacac aacttgttta ttctccagct gtggtaacat ctgttgcatt
    41521 ccaaaatatt ggttaagcga tgcagctccc ccctgtccta aaagataagg acggtaggaa
    41581 cgattgtatt tctcagctaa taccgcagga tgagcaccgt caaaacgaag ccattcacta
    41641 gagccaaaga agggaacaaa acgcacattt ggatcagata gtgctctgac tttttgactt
    41701 cgctccttaa aactatcgat agtagtagcc actgctgaac gcttttcagc tcctagatta
    41761 tgatgcatct cagtaggata aaagaaaatg agcagaaaaa ccaacaaacc agcgatcaag
    41821 accggtccga agatcatcca taagcgttta agcattttgt agctccacaa taccagctat
    41881 gattttatta gctgtattcc agtcgtcacg accaaactct gttacaggga cacgaatgtc
    41941 aaaacggttc tcaatctcca caatcaactc aaccgttccc atactatcca agacacctgc
    42001 atcaaaaaga tcttcatcca tcatgtcaga aacatcttcc ataaacaact catcaataat
    42061 ttcgataact tctgatttga tatccatatt ttatttcctt ttatttttta aaccatagat
    42121 tattcaagaa tccagaaaag attaagaatg acaacatgac aacatggaaa gtgacaacca
    42181 tgccaagcaa ctgaatccag cgattctcag gtagggcagc cttccctgct tttttccgtt
    42241 ccttattgag tgtttttttc ttgcgaaccc aggcatcatt gatgaccaag cctagtccat
    42301 gaaagagtcc ataggcgata tagtaccagg tcacaccatg ccaaaatccc ataatcagca
    42361 tatttacaat gtaggccatg cttgaggtta cattacgatt tttaaagact ttctttctgg
    42421 ttaacaccat caccattcgc ataaagacaa agtcacggaa ccagaaggac agactcatat
    42481 gccagcgatt ccaaaactcc tttaaatccc ttgataaaaa gggcttgtta aagttgatag
    42541 ggctacggat tcccatcaag tttgagatgg ccaaagcaaa catagaataa cctgcaaagt
    42601 caaagaagag ctccagacca aaagtataca taactgccaa ggcatagaga ttaaagaagc
    42661 cacctgactg caaggctaaa ttcttcagag gaggtagtaa ggtctctcct aaaacatgag
    42721 ctaggataaa cttatacaaa aagccccaca tgatatagcg gacagattca tccagcatat
    42781 ccatcaactc atctcgctca ggaatagcct gataattttc attaaatcgc ttaaagcgat
    42841 cgattggacc actcgagaaa gttggcatga agagaaggaa acggaggaat tcccagaggg
    42901 taaaatcctt aatcactcca tctctcagct cgatgataat cccaaccgaa cgaaaggtaa
    42961 gataagaaat tcccaagaac ccaagcaaag actgcgttcc attgatagct ggttgcacct
    43021 tgacaaagat aatcggaagt agggacagaa aactaactaa gtagaagacc cacttgccat
    43081 ccttgctttt tcgataatgc ttgtagaaaa gcaggagcaa tatttcccag caaaggtaaa
    43141 tacccaaggc agctagttga ttggtctttc cacccaccaa catggtgaca ataaagaaga
    43201 gacttaccaa cacttcatac caggcaaagc gtttcttgaa aaagagacct ataaagatgg
    43261 gcaaggttgc agcaatcaca taaacaaaat actgaggatt gccatatggc tctaaatgag
    43321 gaagctgttg aaaaaactcc atcatctctt attcacctcg ttaatcaatc ctttgatgtc
    43381 aatctttcca tttggagtca gtggcaaact gtctcggtaa aggaatttag atggcatcat
    43441 ataggacatc atgatgtctg tcaggtcttc cttgatggcc ttggtaatat cgatatctcg
    43501 ctcaaactgc tcacgaacac cgtcttttaa gatgacataa gccaatagat tttgtacctt
    43561 gtggtccttg ttatagcgcg gtactgcgac agcagattcg ataaagcgag acttgttgag
    43621 gttttgagag acatcttcta actcaatgcg gtaaccgtta aacttaatct ggaagtccat
    43681 gcgtccgccg tagagaagca agccctcatc tgtcatggtt cccacatcgc ctgtgtgata
    43741 ggctggcaga ccttcaaact caaagaaggc ttctgctgtt ttttcaggat tgttcatata
    43801 accttttgaa acagctggcc cagaaacaat gatttctccc tgttcgccat ttggcagttt
    43861 atttccttcc tcgtcaatga taaaggttgg agaatcagcc ttggtatagc cgattggtag
    43921 gcgtttgaga gtcgctaaca tctcgtctgt cacggcaact gctgacagag ctactgtcgc
    43981 ttctgttggg ccgtaagcat tgatgatacg ggcatttggg aaacgcttgc gcagtttttg
    44041 agctgttttg accgtcaatt cttcaccatc aaagtagaaa tgcgtgactc caggcatttt
    44101 ctcactgttg aagtattcag acaacatggc catatctgca aaggatggtg ttgatgtcca
    44161 gatagcgatt ggcaatgaaa agatagccgc aaagagttgc ttaaagtcct gagtgatgac
    44221 tgaaggaaga gtgaaaagcg taccaccaag tgccaaggtc ggtgcccaat acatgacaga
    44281 caagtcaaaa gaataaggtg gctgtgccag catttgcgga cgactcggtg tcgcaaattc
    44341 cttatccata atcatccagt ttgtaaagct gaggagatta tcatgtgaaa tctgcactcc
    44401 cttaggctta ccagtcgtac cagaagtaaa gataatgtag taattatcat ctcccttgac
    44461 tggatgcgtg atttcatagt tattcccttg aacaaaggct tcttgaacct gagctagatt
    44521 tatcattggt gtagaaacct gctccaaggg aaaggctgaa atggcaataa tcaagcttgg
    44581 ctctgctact tctaaaatag ctgaaactcg ctccaaggcc gaatggctat caattggaat
    44641 gtaggcatga cctgacttag tcagcgctac aaaggttgcc aacatttcat attcttggcc
    44701 accaaaaaca accacaggag acttctcagg caagcctagt tggtcaatga ctgcagccaa
    44761 actatccgaa tcagccttta aatcgccata agtgtgttcc tgccccaaaa cattatagac
    44821 aggatagcta ggctgtgtct gagcaaaatg ctcaatggtt tcaatcatat ctgctattgg
    44881 tttatttgac acaataggga ttctccttca agttaaaatt cattatagat aaagcttcct
    44941 tgaccctgac caagatagct aaagaagtaa agcagcccta gaaagataag aaaatacaag
    45001 gctgtccgac caagaaagag gtacaattct tttctctgtt tcatcaagaa aaaccattca
    45061 tttctgtaat ttttcgctaa aataagagtg attcttacta gcttattttt ctaccattgt
    45121 accactttat atagtacctt ttcaattgtt taccgtatgt ttccaataga tttcagctta
    45181 ttttaaggat tatacagttt ttctatgtat attttcaaat agagtgatcc tgcttcaaaa
    45241 ctccatttca ggagacaatg aagtaaatct tcccataata aaacacacaa tatcaagttt
    45301 tttcaacacc tgatactatg cgcttttctg atttttaaag actttttaac cactctctca
    45361 tttaaaataa tctcgtctga tataaattaa aatagcttct atcatcagac aaatggctga
    45421 tagccaaaaa ctgatgctaa taccaaaact ctcagtaata tagctcatta gcaaaacaaa
    45481 tactgaaaat gctaatgtag aaatcacttc aagaacggaa tagacattaa ctaaatgatt
    45541 ttcctctact gtttcctgaa gaaatacact ttcaggaact tcttttagtt gcgataacat
    45601 accaactaaa gctgaaaata ataaaaacat ctgtgcgttt ggaaaatata gaatagtcag
    45661 tgtcactatt gccatagcta caaaatggaa gaaacccaaa tcgtatttga aaaaatttcc
    45721 aataaatttg ctgatacaaa aagtcttaat ctaggatttc tagcaactaa cttccaacct
    45781 ttgagcaaaa tttcaagatt agtttctgac tctaacacct ccacttcagc gttaggaaga
    45841 aataacatca gaaagctaga aatgatatac aagactaaaa tgataaacgt ggtaggtaac
    45901 agaccaattg ttgcaaacaa gagtccacct aatccccaac ctatcaattg aacagcttca
    45961 ccagtcattg ataaggctga attagcctta cccaaatcgg tcgcatagcg tggcacaata
    46021 gcataggaaa cgggtgctgc aaaaccatct agtatggaaa ttgcaacaac aaatagatag
    46081 gtcaccaaag gcgctacgga ttgcattacg gtaaacattc ctaccagtat cgccaataat
    46141 atagtctttc caaattgaga taaagataaa accctattta gcgctaacct tttagtaacc
    46201 aacggaacta aaagactcgc aacaaaagag gatattccta ttaagatagg aactagtgat
    46261 gtggcaatta ctgattttga aataatgtat atgttagcaa tgattgttac tctaaagaaa
    46321 atatctgcta aatttgcaaa taattgagag actaacaact tgacaaatga attagacatg
    46381 tagcgctccc cctttagtta caaactttgg aattaaattt gatttttaat attttagcat
    46441 aaaatagcag aaaacaaaag tgaggttttt tacatgaaaa aacaaaaact attgccttta
    46501 ttattcctaa ttttaggagg aattatgatt atcgttgttg gctagtacta agaagttagt
    46561 tagtagaata attacatctt caatcgaaaa agagactggg aaaaagtctt aaaaatcaga
    46621 aaaacgctca aatcctttgg cgctctaagc ttttctaatg atagatttta gatagtcaaa
    46681 aaaatatctg aaaagttccc aggatcgtcg tctcagcatc aaaaatacgt gaaatttgca
    46741 taattaaaac aaatttcagg ggagt
//
LOCUS       DAVBGY010000011        45241 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVBGY010000011 DAVBGY010000000
VERSION     DAVBGY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45241
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.011"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(<1..197)
                     /locus_tag="VQ620_000618"
     CDS             complement(<1..197)
                     /locus_tag="VQ620_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2763294.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKTKLSLEDLLMATLQYVREYR"
     gene            192..446
                     /locus_tag="VQ620_000619"
     CDS             192..446
                     /locus_tag="VQ620_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET2763295.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIGAEAIRRNCERGAIFSGKIKHHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            790..2124
                     /gene="trmFO"
                     /locus_tag="VQ620_000620"
     CDS             790..2124
                     /gene="trmFO"
                     /locus_tag="VQ620_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET2763296.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRIFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEAIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            2208..2945
                     /locus_tag="VQ620_000621"
     CDS             2208..2945
                     /locus_tag="VQ620_000621"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET2763297.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            2954..3511
                     /gene="frr"
                     /locus_tag="VQ620_000622"
     CDS             2954..3511
                     /gene="frr"
                     /locus_tag="VQ620_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET2763298.1"
                     /translation="MANAIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRIHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVV
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3571..4425
                     /gene="cvfB"
                     /locus_tag="VQ620_000623"
     CDS             3571..4425
                     /gene="cvfB"
                     /locus_tag="VQ620_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HET2763299.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTSLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4434..4649
                     /locus_tag="VQ620_000624"
     CDS             4434..4649
                     /locus_tag="VQ620_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HET2763300.1"
                     /translation="MRKSFYTWLMTERNPKSNNPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4735..5703
                     /locus_tag="VQ620_000625"
     CDS             4735..5703
                     /locus_tag="VQ620_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET2763301.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMALVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            5883..6383
                     /locus_tag="VQ620_000626"
     CDS             5883..6383
                     /locus_tag="VQ620_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2763302.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            6386..6646
                     /locus_tag="VQ620_000627"
                     /pseudo
     CDS             6386..6646
                     /locus_tag="VQ620_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(7013..8124)
                     /gene="ald"
                     /locus_tag="VQ620_000628"
                     /pseudo
     CDS             complement(7013..8124)
                     /gene="ald"
                     /locus_tag="VQ620_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253293.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            8301..8870
                     /locus_tag="VQ620_000629"
     CDS             8301..8870
                     /locus_tag="VQ620_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2763303.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            8938..9588
                     /locus_tag="VQ620_000630"
     CDS             8938..9588
                     /locus_tag="VQ620_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET2763304.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTSVKETNLQ
                     AEVAAVSKDSSTGKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            9572..11812
                     /locus_tag="VQ620_000631"
     CDS             9572..11812
                     /locus_tag="VQ620_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HET2763305.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGVFGFWFLFQTWQQSQVSQNLVDSVERVRILPDTIKVNGDSL
                     SFRGKSDGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFDYQGY
                     LKTQGIYQTLNIKRIQSLQKVGSWDIGENLSSLRRKAVVWIKMHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     VFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVASRPLVFGQPNEWLLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIKKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            11992..12180
                     /locus_tag="VQ620_000632"
     CDS             11992..12180
                     /locus_tag="VQ620_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763306.1"
                     /translation="MTVTKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGGY"
     gene            12212..12799
                     /locus_tag="VQ620_000633"
     CDS             12212..12799
                     /locus_tag="VQ620_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2763307.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            12803..13978
                     /locus_tag="VQ620_000634"
     CDS             12803..13978
                     /locus_tag="VQ620_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763308.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWKDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKRIF"
     misc_feature    14152..14283
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14299..14829
                     /gene="infC"
                     /locus_tag="VQ620_000635"
     CDS             14299..14829
                     /gene="infC"
                     /locus_tag="VQ620_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET2763309.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            14862..15062
                     /gene="rpmI"
                     /locus_tag="VQ620_000636"
     CDS             14862..15062
                     /gene="rpmI"
                     /locus_tag="VQ620_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET2763310.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            15114..15473
                     /gene="rplT"
                     /locus_tag="VQ620_000637"
     CDS             15114..15473
                     /gene="rplT"
                     /locus_tag="VQ620_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET2763311.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15531..15911
                     /locus_tag="VQ620_000638"
     CDS             15531..15911
                     /locus_tag="VQ620_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2763312.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            16161..16961
                     /locus_tag="VQ620_000639"
     CDS             16161..16961
                     /locus_tag="VQ620_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HET2763313.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVNQRVCEDGPVFRTGTVVL"
     gene            16924..17910
                     /locus_tag="VQ620_000640"
     CDS             16924..17910
                     /locus_tag="VQ620_000640"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET2763314.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            17952..19853
                     /locus_tag="VQ620_000641"
     CDS             17952..19853
                     /locus_tag="VQ620_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HET2763315.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYM
                     AKNETVDGYQLGSDGKWLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWL
                     DKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIP
                     VASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINN
                     SLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYL
                     SAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMK
                     INEKLGGKD"
     gene            complement(19914..21569)
                     /gene="pavA"
                     /locus_tag="VQ620_000642"
     CDS             complement(19914..21569)
                     /gene="pavA"
                     /locus_tag="VQ620_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HET2763316.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNCQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPLANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            21674..22171
                     /gene="ybeY"
                     /locus_tag="VQ620_000643"
     CDS             21674..22171
                     /gene="ybeY"
                     /locus_tag="VQ620_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET2763317.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLGYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            22152..22547
                     /locus_tag="VQ620_000644"
     CDS             22152..22547
                     /locus_tag="VQ620_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET2763318.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            22564..23463
                     /gene="era"
                     /locus_tag="VQ620_000645"
     CDS             22564..23463
                     /gene="era"
                     /locus_tag="VQ620_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET2763319.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPDRFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            23512..24336
                     /gene="mutM"
                     /locus_tag="VQ620_000646"
     CDS             23512..24336
                     /gene="mutM"
                     /locus_tag="VQ620_000646"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HET2763320.1"
                     /translation="MPELPEVETVCRCLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQGCVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24336..24941
                     /locus_tag="VQ620_000647"
     CDS             24336..24941
                     /locus_tag="VQ620_000647"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET2763321.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFWQEIILENGELNRPLLASLIFSNPDEREWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            24928..26127
                     /locus_tag="VQ620_000648"
     CDS             24928..26127
                     /locus_tag="VQ620_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HET2763322.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            26127..26276
                     /gene="rpmG"
                     /locus_tag="VQ620_000649"
     CDS             26127..26276
                     /gene="rpmG"
                     /locus_tag="VQ620_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2763323.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26316..26549
                     /gene="secG"
                     /locus_tag="VQ620_000650"
     CDS             26316..26549
                     /gene="secG"
                     /locus_tag="VQ620_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET2763324.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26651..29005
                     /gene="rnr"
                     /locus_tag="VQ620_000651"
     CDS             26651..29005
                     /gene="rnr"
                     /locus_tag="VQ620_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HET2763325.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSHSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            28968..29435
                     /gene="smpB"
                     /locus_tag="VQ620_000652"
     CDS             28968..29435
                     /gene="smpB"
                     /locus_tag="VQ620_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET2763326.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            29451..30311
                     /gene="tehB"
                     /locus_tag="VQ620_000653"
     CDS             29451..30311
                     /gene="tehB"
                     /locus_tag="VQ620_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HET2763327.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEDVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            30384..31337
                     /gene="coiA"
                     /locus_tag="VQ620_000654"
     CDS             30384..31337
                     /gene="coiA"
                     /locus_tag="VQ620_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HET2763328.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKKQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            31356..33158
                     /gene="pepF"
                     /locus_tag="VQ620_000655"
     CDS             31356..33158
                     /gene="pepF"
                     /locus_tag="VQ620_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET2763329.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEVLKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTFYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGIYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELCSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            33160..33873
                     /locus_tag="VQ620_000656"
     CDS             33160..33873
                     /locus_tag="VQ620_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET2763330.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELENFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            33940..34881
                     /gene="prsA"
                     /locus_tag="VQ620_000657"
     CDS             33940..34881
                     /gene="prsA"
                     /locus_tag="VQ620_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HET2763331.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(34998..35339)
                     /locus_tag="VQ620_000658"
                     /pseudo
     CDS             complement(34998..35339)
                     /locus_tag="VQ620_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            35752..35847
                     /locus_tag="VQ620_000659"
                     /pseudo
     CDS             35752..35847
                     /locus_tag="VQ620_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(36100..36720)
                     /locus_tag="VQ620_000660"
     CDS             complement(36100..36720)
                     /locus_tag="VQ620_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2763332.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQDASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(36720..37202)
                     /locus_tag="VQ620_000661"
     CDS             complement(36720..37202)
                     /locus_tag="VQ620_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HET2763333.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(37202..38044)
                     /locus_tag="VQ620_000662"
     CDS             complement(37202..38044)
                     /locus_tag="VQ620_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HET2763334.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(38139..38939)
                     /locus_tag="VQ620_000663"
     CDS             complement(38139..38939)
                     /locus_tag="VQ620_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HET2763335.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSHGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            39083..40462
                     /gene="glmU"
                     /locus_tag="VQ620_000664"
     CDS             39083..40462
                     /gene="glmU"
                     /locus_tag="VQ620_000664"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET2763336.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            40472..41017
                     /locus_tag="VQ620_000665"
     CDS             40472..41017
                     /locus_tag="VQ620_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2763337.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            41029..41343
                     /locus_tag="VQ620_000666"
     CDS             41029..41343
                     /locus_tag="VQ620_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HET2763338.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            41360..42052
                     /locus_tag="VQ620_000667"
     CDS             41360..42052
                     /locus_tag="VQ620_000667"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HET2763339.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            42129..42620
                     /gene="rocS"
                     /locus_tag="VQ620_000668"
     CDS             42129..42620
                     /gene="rocS"
                     /locus_tag="VQ620_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HET2763340.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            42718..43308
                     /locus_tag="VQ620_000669"
     CDS             42718..43308
                     /locus_tag="VQ620_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET2763341.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGVLDLSQLFD"
     gene            43317..45080
                     /locus_tag="VQ620_000670"
     CDS             43317..45080
                     /locus_tag="VQ620_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET2763342.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT        13682 a         8283 c        10066 g        13210 t
ORIGIN      
        1 cgatattctc gcacatattg aagagtggcc ataagaaggt cttctaggct taatttagtt
       61 tttcgtccac cttttgcgtg tttaagttga taagctgttt ttaatacagc taatatctct
      121 tcaaaagtcg tgcgctgaac accaacaaga cgcttaaatc gtgcatcagt tagttgttta
      181 cttgcttcat aattcataga actactatac catattttgt ttcgcaggaa gtctattgga
      241 aagtaagaaa tattggagct gaggcgatta gaagaaattg tgagcgtggt gctatttttt
      301 caggtaaaat aaaacatcac gaagattctc agtttaaagg agaccactat gttgaatgtt
      361 atgctgtttt agataatacg gttatagcaa gagatagaat aacagtccct atcgatccgt
      421 tatgtggaaa agattttata gagtagcata taattgattc ttaactggaa tactctctat
      481 ctctttacat caagaaaatg actaaacagg gaagtttgcc ttcttccctt tttttgttat
      541 actagtagaa gaaaaaaata gaaagatttg tgggagtgaa aaagtccggg ggactttttc
      601 agcttgagcc aagaaattcg agagcgagga aagtccgggg gactttttca gcctgagcca
      661 agaaattcga gagcgaagta agtccaatgg actttttcag cctgagccaa gaaactcgaa
      721 agctcgtaag tctgattggc tttttcaatg tgaatcttaa cttcacactc ccaaagaggt
      781 attagtgtcg tgtctcaatc ttatatcaat gttatcggtg ctggtttggc aggttctgaa
      841 gcagcttacc aaatcgcaga gcgtggtatt ccagttaaac tatatgaaat gcgtggtgtc
      901 aagtcgactc cccagcataa aacagacaat tttgctgagt tggtttgttc caattctttg
      961 cgtggggatg ctttgacaaa tgcagttggt cttctcaagg aagaaatgcg tcgcttgggt
     1021 tctgttatct tggaatctgc tgaggctaca cgtgttcctg caggtggtgc ccttgcagtg
     1081 gaccgtgatg gtttctctca aatggtgacc gaaaaagttg ccaaccaccc cttgattgaa
     1141 gtggttcgtg atgaaattac agaattgccg acagatgtta ttacggttat cgctactggt
     1201 cctttgacaa gtgatgcctt ggctgaaaag attcatgccc ttaatgacgg tgatggtttt
     1261 tatttctacg atgcggcagc gcctattatc gatgtcaaca ctatcgatat gagcaaggtc
     1321 tacctcaaat cacgttatga taagggagaa gcggcctacc tcaatgcccc tatgaccaag
     1381 caagaattta tggatttcca tgaagctttg gtcaatgcag aagaagcacc gcttaattct
     1441 tttgaaaaag aaaagtactt tgaaggctgt atgcctatcg aagtcatggc caaacgtggc
     1501 attaaaacta tgctttatgg ccctatgaag ccagtcggtc ttgagtaccc agacgactat
     1561 acaggacctc gtgatggaga atttaaaaca ccttatgcgg ttgtgcaact tcgtcaggac
     1621 aatgcagctg gtagcctcta caatattgtt ggtttccaga cccacctcaa atggggagaa
     1681 caaaagcgta tcttccaaat gattccgggt cttgaaaatg cagagtttgt ccgttatggt
     1741 gtcatgcatc gcaattctta catggattca ccaaatcttc ttgagcagac ttaccgttct
     1801 aagaaacaac caaatctctt ctttgctggt cagatgacgg gggtggaagg ctatgttgag
     1861 tcggcggctt caggcttagt tgcgggaatt aacgcagctc gtctcttcaa ggaagaaagc
     1921 gaggctattt tccccgagac gacagcgatt ggaagcttag ctcattacat tacccatgcc
     1981 gacagcaaac atttccaacc aatgaatgtc aattttggga tcatcaagga gttggaaggc
     2041 gagcgtatcc gtgataagaa ggctcgttat gaaaaaattg cagagcgtgc ccttgccgac
     2101 ttagaggaat ttttgactgt ctaatttttt tgaaagaatt gctcatgata ctataaaaat
     2161 cttagaaatt gtgataaaat aggtaggatg aaagaaggag agtgaaaatg gcgaatccca
     2221 agtataaacg tattttaatc aagttatcag gtgaagccct tgccggtgaa cgtggcgtag
     2281 ggattgatat ccaaacagtt caaacaatcg caaaagagat tcaagaagtt catagcttag
     2341 gtatcgaaat tgcccttgtt attggtggag gaaatctctg gcgtggagaa cctgcagcag
     2401 aagcaggtat ggaccgtgtt caggcagatt acactggaat gcttgggact gttatgaatg
     2461 ctcttgtgat ggcagattca ttgcaacaag ttggggttga tacgcgtgta caaacagcta
     2521 ttgctatgca acaagtggca gagccttatg tccgtggacg tgcccttcgt caccttgaaa
     2581 aaggccgtat cgttatcttt ggtgctggaa ttggttcacc atacttctcg acagatacaa
     2641 cagcggccct tcgtgcagct gaaatcgaag cggatgccat cctcatggct aaaaatggtg
     2701 tcgatggtgt ttacaatgcc gatcctaaga aagataagac agctgttaag tttgaagaat
     2761 tgacccaccg tgacgttatc aataaaggtc ttcgtatcat ggactcaaca gcttcaaccc
     2821 tctcaatgga caacgacatt gacttggttg tcttcaacat gaaccaatca ggcaacatca
     2881 aacgtgtcgt atttggtgaa aatatcggaa caacagtttc aaataatatc gaagaaaagg
     2941 aataagaaag aatatggcta acgcaattat tgaaaaagct aaagagagaa tgacccagtc
     3001 tcaccaatca cttgctcgtg aatttggtgg tatccgtgct ggtcgtgcca atgcaagctt
     3061 gcttgaccgt atacatgtag aatactatgg agttgaaact cctcttaacc aaatcgcttc
     3121 aattacgatt ccagaagcgc gtgttttgtt ggtaacacca tttgacaagt cttcattgaa
     3181 agacatcgaa cgtgccttga acgcttctga tcttggtatc acaccagcta atgacggttc
     3241 tgtgattcgc ttggttgtcc cagctcttac agaagagact cgtcgtgacc ttgctaaaga
     3301 agtgaagaag gtcggcgaaa atgctaaagt ggctgtccgc aatatccgtc gcgatgctat
     3361 ggacgaagct aagaaacaag aaaaagcaaa agaaatcact gaagacgaat tgaagactct
     3421 tgaaaaagac attcaaaaag taacagacga tgctgttaaa cacatcgacg atatgactgc
     3481 caacaaagag aaagaacttt tggaagtcta aaaataaaca gaaaaactca gttggcattg
     3541 ctggctgagt tttattcgaa agaaggaaat atgaatacaa atcttgcaag ttttatcgtt
     3601 ggactgatca tcgatgaaaa cgaccgtttt tactttgtgc aaaaggatgg tcaaacctat
     3661 gctcttgcta aggaagaagg ccaacataca gtaggggata cggtcaaagg ttttgcatac
     3721 acggatatga agcaaaaact ccgcctgaca agcttagaag tgactgccac tcaggaccaa
     3781 tttggttggg gacgtgtcac agaggttcgt aaggacttgg gtgtctttgt ggatacaggc
     3841 cttcctgaca aggaaatcgt tgtgtcactc gatattctcc ctgagctcaa ggaactctgg
     3901 cctaagaagg gcgaccaact ctacatccgt cttgaagtgg ataagaaaga ccgtatctgg
     3961 ggtctcttgg cttatcaaga agacttccaa cgtcttgctc gtcctgccta caacaacatg
     4021 cagaaccaaa actggccagc cattgtttac cgtctcaagc tatcaggaac ttttgtttac
     4081 ctaccagaaa ataatatgct tggttttatt catcctagcg agcgttacgc agagccacgt
     4141 ttggggcaag tattagatgc gcgcgttatt ggtttccgtg aagtggaccg cactttgaac
     4201 ctctccctca aaccacgctc ctttgaaatg ttggaaaacg atgctcagat gattttgact
     4261 tatttggaaa gcaatggcgg tttcatgacc ttaaatgaca agtcatctcc agacgacatc
     4321 aaggcaacct ttggcatttc taaaggtcag ttcaagaaag ctttaggtgg tcttatgaag
     4381 gctggtaaaa tcaagcagga ccagtttggt acagagttga tttagggagg cttatgagaa
     4441 aatcatttta cacttggctc atgaccgagc gcaatcctaa aagtaacaat cccaaagcaa
     4501 ttttggcaga cctcgctttt gaagaggcag cttttccaaa acatacagat gattttgatg
     4561 aggtcagtcg ctttttggag gagcatgcca gtttctcttt taacctagga gattttgaca
     4621 gcatttggca ggaatatcta gaacactagc atttattcat tgggtttggg ctagtaattt
     4681 ctccatccct ctgctataat aaaaagaaat aaaaggatta gagaggttct ttatttgaag
     4741 gaacattcaa tagacattca actgagtcat ccagatgacc tgtttcatct ttttggttcc
     4801 aatgaacgcc atcttcgttt gatggaagaa gagcttgatg ttgtgattca tgctcgtacg
     4861 gagattgtcc aggttttggg agaagagtct gcttgcgagg aagcccgtca ggttattcaa
     4921 gctctcatgg ccttggtaaa tcgtgggatg accgttggta cgccagatgt agtgacggct
     4981 attagtatgg tcaaaaatga tgaaattgac aagtttgtcg ccctttacga agaagaaatc
     5041 atcaaggata atactgggaa gcctatccgc gtcaaaacct taggtcaaaa actttatgtg
     5101 gacagtgtca aacagcatga tgtgaccttt ggaattgggc cagcaggtac agggaagacc
     5161 ttccttgcag tgaccttggc agtgactgcc cttaaacgtg ggcaagtcaa gcgaattatc
     5221 ctaactcgtc cagcggtgga agcgggagag agtcttggat ttcttccggg tgatcttaag
     5281 gagaaggtgg atccttacct tcgtcctgtt tacgatgcct tgtatcaaat tcttgggaaa
     5341 gaccaaacga ctcgtctcat ggagcgtgaa attatcgaaa ttgcgcccct tgcctatatg
     5401 cgtggccgga ccttggatga tgcctttgtc attctcgatg aggcgcaaaa cacgaccatc
     5461 atgcagatga agatgttctt gacgcgttta ggctttcatt ctaagatgat tgtcaatgga
     5521 gatatcagtc agattgacct gccacgtaat gtcaagtccg gtttgattga tgctcaagag
     5581 aaactcaaga acatccatca gattgacttt gttcattttt cagccaagga tgtggttcgc
     5641 catccagttg tcgctcagat tatccgagcc tatgaatatt ctactgaagt tgcacacgac
     5701 tgattttgag gaagttcgcc tgcaaaagaa tatacttttg agagaggaaa atccagttgt
     5761 ataggctaaa ggtttgagac aacgattagg cacgatggaa agaactttta tgtggctgat
     5821 gacgatcagt gcatcttcct gtgtcataat cacagggcac aagaaagtag gaatttgaaa
     5881 agatgattga ccaactatct aagtattaca gttgtaggat actaactgaa aaggatattc
     5941 caagtatttt atctttatat gaaagtaatc ctctgtattt tcagcattgt ccaccagagc
     6001 caaattttgc aactgtaaaa gaggacatgc tttgtctacc tgaaggtaaa gctaaggctg
     6061 ataagttttt tgttggattt tggaatggct ctgaccttgt ggctgttatg gattttgtct
     6121 atgcatatcc tgatgaggag actgttttta ttggtttgtt tatggttgat caagcctatc
     6181 agaggaaagg gattggtagt catattgtga cagaagcact agcttatttt gctaagaact
     6241 ttcgaaaggc acgtttggct tatgttaagg gaaatccgca atctcagcat ttttgggaaa
     6301 agcagggctt taaatcaatt ggatgcgagg ttaagcaaga actctatacg gttgttatcg
     6361 ctgaacagag cctagaagat tagaaatggc atcaagtaag aactatttgg aatttgtttt
     6421 ggaacaatta tcaggattag atgatgtgac ttaccgttcc atgattgggg agtatattct
     6481 ttacttccgc ggcaagatta ttggcggcat ttatgacgat cgctttttag ttaaacccgt
     6541 gcaagcagtc ttagataaga ttgaccaatc ttcttttgag tttccataca aaggtgccaa
     6601 agaaatgatt tgagtggaag aacttgataa taagatgttt ctataagacc tgattttagc
     6661 tatgtataac caactgccaa cgcccaaacc taaaaagaaa aagcaagggt gaacgaagta
     6721 aaaaagaagt ctgctaaggc cctgtctttg cacgggtaaa attttatata taaaaagaag
     6781 ctgggactaa agagctcagc ttcctttggt ttatataatt gtcattacaa gacgaagtgg
     6841 ttgggcgaaa ctctgttgac tttattcaat ttagagtttc ttatgcacaa ttgagtctgg
     6901 aacgaaagtc tccagttgca aagtatacag tacaataaac caacgatgta atagctgatg
     6961 acacaaagca cagtgggtag gacttgcgaa gtcacccttt tcttttcaaa atttatacta
     7021 aatcattgat atcagtgtag tcacgattaa gtccttgagc aactggtagg ctagtcaagt
     7081 aaccttgata agtggtcaca ccttgacgca agccttcatc ttcagagatt gcttgtgcga
     7141 atcctttgcc agccaaagct tcgatataag gaagagtgac attggttagg gcgatggttg
     7201 aagtgcgggc aaccgcacca gggatattgg caacggcata gtggagaaca ccgtgttttt
     7261 catagacggg ttcatcgtgc gttgtcacac ggtcagctgt ttcgataacg ccaccttggt
     7321 caacagcaac gtcaacgata cagagcctgg acgcatttgt ttgaccatct catctgtcac
     7381 caattccggt gcttttgcac cagggatgag aatggctcca atcaccacat cagcatctct
     7441 cacacttgct tcaatgttga atgaattaga tataatagtt tgaatttgac ttccaaagac
     7501 ttcttctaga actgagagac gcttggcact aatatctaaa atagtcactt gagcaccaag
     7561 accaagggcg atgcgggcag catgtgtacc gacgacacca tcaccgatga tagttacttt
     7621 tccttttgga acacctggta caccaccaag tagaacacca gagccaccag cttgcttagt
     7681 aaggaagtga gctccgattt gaacagccat acgacctgca acctcactca taggaacgag
     7741 gagcggtagt tgtccttgat tgtcacgaac agtttcatag gcaattcctg ttgtttttgc
     7801 tgctaacata gcatctgcta attctggagc agcggccatg tgcaagtagg tgaagagaag
     7861 aagatcgtcg cgcaagtaac cgtattcaga acttaaaggt tcttttattt tcacaaccaa
     7921 ctctgctgcc caagcttcac cagcagtagc gacaatctca gctccttgct tttgatagtc
     7981 agcatcagta aagccagaac cgagaccagc atttgtttcg ataaggacac gatgaccacg
     8041 actaactaag ctatgaacac ctgcaggtgt gagggcgaca cggttttcgt tatttttaat
     8101 ttcttttggg attccgatta acattgagat aacctacctt tcaattgacg gtcttgtttt
     8161 ggttgtcaca ttccagttca taaatcaaaa atgtgacggt ttcattgtat atgaaaccgc
     8221 ttcaaaaatc aagaaaaact tgtcatccaa atttttttat gctagactag tgaaaatcaa
     8281 gctctaatgg agggaaaagt atggaatcaa tatttgtgaa atttgcccag tatccgtcta
     8341 tagaaacgga gcgtttattg cttagacctg taactttgga tgatgcggaa gcaatgtttg
     8401 actatgcctc ggacaagggt aatacacgtt acacttttcc aaccaatcaa agcttggaag
     8461 aaaccaagaa taacattgct cagttctact tggctaatcc cttgggacgt tgggggattg
     8521 aattaaaaga taacggcaag tttatcggaa ctattgactt acacaagatt gatcctgttc
     8581 ttaagaaggc agctattggc tacattatca ataaaaagta ttggaatcaa ggattaacga
     8641 cagaagccaa tcgtgctgtg attgagctag cttttgagaa gatagggatg aataagttga
     8701 ctgcccttca cgataaggct aatcccgcgt caggaaaggt catggagaaa tcaggcatgc
     8761 gtttttccca tgcagaacca tatgcttgta tggaccagca tgaaaaaggc cgaatcgtga
     8821 caagagttca ttatgtcttg accaaggaag actattttgc aaataaataa gcagttgaaa
     8881 agaaattttt cgactgtttt ttcttcctct tacgaataat ctaagagagg agaaaatatg
     8941 gaagcaatta tcgagaaaat caaagagtat aaaatcatcg tcatctgtac tggtctgggc
     9001 ttgcttgtag gaggattttt cctgctaaag ccagctccac aaacatctgt caaagagacg
     9061 aatttgcagg ctgaagttgc agctgtttcc aaggactcat cgaccggaaa ggaagtgaac
     9121 aaggaagaga aggaagaacc agttgaacaa gatctaatca cagtagatgt caaaggtgct
     9181 gtcaaatcgc cagggattta tgacttgcct gtaggtagtc gagtcaatga tgctgttcag
     9241 aaggctggtg gcttgacaga gcaagcagac agcaagtcgc tcaatctagc tcagaaagtt
     9301 agtgatgagg ctctggttta cgttcctact aagggagaag aagcagttag tcaacagact
     9361 ggttcgggga cagcttcttc aacaagcaag gaaaagaagg tcaatctcaa caaggccagt
     9421 ctggaagaac tcaagcaggt caagggactg ggaggaaaac gagctcagga cattattgac
     9481 catcgtgagg caaatggcaa gttcaagtca gtagacgagc tcaagaaggt ctctggcatt
     9541 ggtgccaaga ccatagaaaa acttaaagac tatgttacag tggattaaga atttctctat
     9601 tcccctaatt tacctgagtt ttctattact atggctttac tacgccattt tctcagtatc
     9661 ctattttgct ttgttgggtt ttgtttttct gctagtctgc ctctttatcc aatttccttg
     9721 gaaatcagca ggtaaagttc tagtgatttg tggagtcttt ggcttctggt ttctgtttca
     9781 aacttggcaa cagagtcaag tgagtcaaaa tttggtggat tctgttgaaa gggtacggat
     9841 tttaccagac actattaagg ttaacggtga cagtctgtcc tttcgtggta agtctgatgg
     9901 tcgtgctttc caagtctatt ataaactcca gtccgaggag gagaaagaag cctttcaagc
     9961 tttaactgac cttcatgaga taggactaga agggaagctt tcggagccag aagggcagag
    10021 aaattttggt ggctttgact accaaggcta tctgaagact cagggaattt accagacact
    10081 caatattaaa agaatccagt cactccaaaa ggttggcagt tgggatatag gtgaaaacct
    10141 gtccagttta cgtcgaaagg ctgtggtttg gattaagatg cacttcccag accctatgcg
    10201 caattacatg acaggactct tgctaggaca tctggacacc gattttgagg agatgaatga
    10261 gctttattcc agtttaggaa ttattcacct ttttgccttg tcaggtatgc aggtagggtt
    10321 tttcatggac ggatttaaga aactactttt acgattgggc ttgacacaag aaaagttgaa
    10381 gtggctgact tatccctttt cccttatcta tgcgggacta actggatttt cagcatcggt
    10441 tattcgcagt ctcttgcaaa agctactggc tcaacatggg gttaagggct tggataattt
    10501 tgccttgacg gtgcttgtcc tctttattgt catgccaaac tttttcttga cagcaggagg
    10561 agtcttgtcc tgtgcttatg cttttatcct gaccatgacc agcaaagaag gggaggggct
    10621 caaggctgtt actagtgaaa gtctagtcat ctccttgggc atattgccca ttctatcctt
    10681 ctattttgcg gaatttcaac cttggtctat ccttttgacc tttgtctttt cctttctttt
    10741 tgacttggtc ttcttaccgc tcttgtctat cttatttgtc ctttcctttc tctatccagt
    10801 cattcagctg aactttatct ttgaatggtt agagggcatt attcgcttgg tctcgcaggt
    10861 ggcaagtaga ccgcttgtct ttggacaacc caatgaatgg cttttaatcc tattgttaat
    10921 ttctttggct ttggtctatg atttgaggaa aaacattaaa aagctaacgg tattgtgctt
    10981 attgattaca gggctctttc tcctgaccaa gcatccactg gaaaatgaaa tcaccatgct
    11041 ggatgtgggg caaggagaaa gtattttcct acgggatgta actgggaaaa ccattctcat
    11101 agatgtaggt ggtaaggcag aatcttataa gaaaatcaaa aaatggcaag aaaagatgac
    11161 gaccagcaat gcccagcgaa gcttgatacc ctatctcaaa agtcgaggag tagctaagat
    11221 tgaccagcta attttgacta acacggacaa ggagcatgtt ggagatttgt cagagatgac
    11281 caaggctttc catgtagggg agattctagt atcaaaagac agtctgaaac agaaggaatt
    11341 tgtggcagaa ctacaggcga ctcaaacaaa ggtgcgtagt atgatagtag gggagaactt
    11401 gcccattttt ggaagtcagt tagaagttct atctccaagg aaaatgggag atggaggaca
    11461 cgatgatacc ctagttctgt atgggaaatt cttggataag caatttctct tcacgggaaa
    11521 tttggaggag aaaggagaga aggacttgct gaagcactat ccagacttga aagtaaatgt
    11581 tttgaaagct agccaacatg gcaataaaaa atcatcaagt ccagcctttc tagaaaaact
    11641 caaaccagag cttactctta tctcagttgg aaagagcaat cgaatgaaac tcccccatca
    11701 ggaaacattg acacgactgg aaggtatcaa tagcaaagtt tatcgaactg accagcaagg
    11761 agctatacgt tttaaggggt tggatagttg gaaaatcgaa agtgttcgat aggaaggata
    11821 aatgttgtag attagtgaaa taaactaaaa atttgttgca taataatgat aaaaacggca
    11881 taatgaaaac gtattcaata ttgaggatat aaaatcatta aaaatcagca aaagttgttt
    11941 tagttagtta gtttataatc tattggtctt cttcagtcca gtgtatctgc tgtgacagtc
    12001 actaaaagtt acaagtatga ttggaatacg gttttggaat atagtaccaa ctatcacgac
    12061 catcagtatg cttggattcc ggaatggtct cgttattaca gctattctga gtataaagtt
    12121 ggcggaggct ggaactacgc tcgttatgag gtcataaact actatactgg aggttattaa
    12181 tccttaaaga gtgagaaaag gagggctgga tatgttaaat cttactcatg ttaccttaaa
    12241 aacgcgacaa gtcatcttgc aagatgcgga ttttaccttt aaaaagggta ggatttatgg
    12301 ccttcttgct atcaatggct cgggaaagac gacactattc cgagctatga gcaagttgct
    12361 tccccttagt agtggacaca tcgcagttcc tccttctttg ttttattatg agagcgttga
    12421 atggctggat ggaaacttaa gtgggatgga ctaccttcgt ctcataaaaa acatctggaa
    12481 gtcagaccta aacttgagag atgaaatcgc ctactgggaa atggctgact atatcagtct
    12541 tcccatccgc aagtattcct tagggatgaa gcaacgcttg gtgattgcta tgtattttct
    12601 cagtcaggcc aaatgctggc tcatggatga gattacaaat ggcttagacg agtattatcg
    12661 acagaagttt tttgataggc tagcacaaat cgatagacaa gaacagctgg ttcttttaag
    12721 ttcccactat aaggaagagt tggttgatat ctgcgataga gtagtaacca ttcatcaggg
    12781 gcagatagaa gaggtttagt ttatgaaaga tgttagtcta tttttattga aaaaagtttt
    12841 caaaagtcgt ttaaactgga ttatcttagc tttatttgta tctgtactcg gtgttacctt
    12901 ttatttaaat agtcagactg caaactcaca cagcttggag agcaggttgg aaagtcgcat
    12961 tgcagccaac gagagggcta tcaatgaaaa tgaagagaaa ctctcccaaa tgtctgatac
    13021 cagctcggag gaataccagt ttgctaaaaa taatttagac gtgcaaaaaa atcttttgac
    13081 gcgaaagaca gaaattctaa ctttactaaa agaagggcgc tggaaagaag cctactatct
    13141 gcagtggaaa gatgaagaga agaattatga atttgtatca aatgacccga ctgctagctc
    13201 tggcttaaaa atgggggttg accgcgaacg gaagatttac caagccctgt atcccttgaa
    13261 cataaaagca catactttgg agtttccgac ccacgggatt gatcagattg tctggatttt
    13321 agaggttatc atcccaagct tgtttgtggt tgcgattatt tttatgctaa cacaactatt
    13381 tgcagaaaga tatcaaaatc atctggacac agctcactta tatcctgttt caaaagtgac
    13441 atttgcaata tcctctcttg gagttggagt gggatatgta actgtgctgt ttatcggaat
    13501 ctgtggcttt tcttttctag tgggaagtct gataagtggt tttggacagt tagattatcc
    13561 ctacccaatt tatagcttag tgaatcaaga agtaactatt gggaaaatac aagatgtatt
    13621 atttcctggc ttgctcttag ctttcttagc ctttatcgtc attgtggaag ttgtgtactt
    13681 gattgcttac tttttcaagc aaaaaatgcc tgtcctcttt ctttcactca ttgggattgt
    13741 tggcttattg tttggcatcc aaacaattca gcctcttcaa aggattgcac atctgattcc
    13801 ctttacttac ttgcgttcag tggagatttt atctggaaga ttacctaagc agattgataa
    13861 tgtcgatcta aattggagca tgggaatggt cttacttcct tgcctgatta tctttttgct
    13921 attgggaatt ctatttattg aaagatgggg aagttcacag aaaaaaagaa ttttttaata
    13981 gattctagct ttcctatagg tagggaaaat aagtaaaaac taacatagag agggaatcaa
    14041 cttgattctc tctttttgat tcgaaaacca aaccaaaata caaacacaaa cttttcaaaa
    14101 aataactttt tatcttgaca agagctagaa aacttggtat catataaaag ttgagaaaag
    14161 cagaagtgag agcttctcgc cttgtgacat taagttgcct ggccctacgg atgaaaagtt
    14221 tcgaagaaac gctatcataa cgtgcgggct tgtatattta cgagtccgct attgtttttc
    14281 tctaataaaa caaaagaggt gaaaaccata gcaaagcaag acttattcat caatgatgag
    14341 attcgtgtac gtgaagttcg cttgattggt cttgaaggag aacagctagg catcaagcca
    14401 ctcagtgaag cgcaagcttt ggctgataac gctaatgttg acctagtatt gattcaaccc
    14461 caagccaaac cgcctgttgc aaaaattatg gactacggta agttcaaatt tgagtaccag
    14521 aagaagcaaa aagaacaacg taaaaaacaa agcgttgtta ctgtgaaaga agttcgtcta
    14581 agtccaacta ttgacaaggg tgactttgat acaaaacttc gtaatgcacg taagttcctt
    14641 gaaaaaggaa ataaagttaa ggtatctatt cgctttaagg gtcgtatgat tacccataaa
    14701 gagattggtg caaaagtttt agccgagttt gctgaagcaa ctcaagatat tgccatcatc
    14761 gaacaacgtg ctaaaatgga tggacgtcaa atgttcatgc aattggcgcc agcgactgac
    14821 aaaaaataat tgtcagaaag ttaaataaag gagaaaaaat tatgccaaaa caaaaaacac
    14881 accgcgcatc agctaaacgt ttcaaacgta caggttctgg tggacttaaa cgtttccgtg
    14941 cttacacttc tcaccgtttc cacggaaaaa ctaagaaaca acgtcgtcat cttcgtaaag
    15001 catctatggt gcattcagga gattacaaac gtatcaaagc aatgcttact cgcttgaaat
    15061 aatagcttga ctgtaagtaa acaattctag gaaatatttg gaggaaataa aacatggcac
    15121 gtgttaaagg tggcgttgta tcacgcaaac gtcgtaaacg tattcttaaa ttagcaaaag
    15181 gttactatgg agctaaacac atcttgttcc gtactgcaaa agaacaagta atgaactctt
    15241 actactatgc ataccgtgac cgtcgtcaga aaaaacgtga cttccgcaaa ttgtggatca
    15301 ctcgtatcaa tgcggcagct cgtatgaacg gactttcata ctcacaattg atgcatggtt
    15361 tgaaattggc tgagatcgaa gttaaccgta aaatgcttgc tgacttggct gttaacgatg
    15421 cagtagcttt cacagctctt gcagatgcag ctaaagcaaa acttggtaaa taacaactga
    15481 tgagactgga acaggattgt ccagtctttt taaaaataaa ggagaaaaat atggcttcaa
    15541 aaatgctaca cacttgctta cgagtagaaa atcttgaaaa atcaatcgca ttttatcaag
    15601 atgcttttgg ttttaaagaa ttgcgtcgca gagattttcc agaccatgcc ttcacgattg
    15661 tctatctagg tcttgagggt gatgactatg agttggagtt gacttataac tacgatcacg
    15721 gtccgtatgt ggttggtgat gggtttgccc atatcgccct cagtacacct gatcttgagg
    15781 cacttcatca agaacacagt gcaaaaggct atgaagtgac tgagccaaat ggtctaccag
    15841 gaactacacc aaactattac tttgtcaaag accctgatgg atataaggtc gaagtcattc
    15901 gtgaaaaata atttcgtgca agttaagtag aatattcgtt ttttacttga ctttttttag
    15961 ctgaaagagt atactaataa aaattcaacc tttaagggga gtccagagag actcacaagg
    16021 tgtcagataa aagaatagtg caattttcta gaggagactt tttgagtgtg ctctcttgtg
    16081 ttgtacgatt ttaactgagg ccttgcacta gcaaggtctt ttctttatct ggtcccctta
    16141 aaatttaagg aggaaaagtt atgaatccca catgtaagaa gcgtttgggt gtcattcggt
    16201 tggaaaccat gaaggtggtt gcacaagagg aaatcgcgcc agcaatcttt gaattagtcc
    16261 tagaaggaga aatggttgaa gccatgcgag caggccaatt tcttcatctg cgtgtaccag
    16321 acgatgccca tcttttacgt cgtcctattt caatttcgtc tattgacaag gcaaacaagc
    16381 agtgtcacct catttatcgg attgagggag ctgggactgc aattttttca accttaagtc
    16441 agggagacac tcttgatgtg atggggcctc agggaaatgg ttttgacttg tctgaccttg
    16501 atgagcagaa tcaggttctc cttgttggtg gtgggattgg tgttccaccc ttgcttgagg
    16561 tggccaagga attgcatgaa cgtggagtga aagtagtgac agtcctcggt tttgctaata
    16621 aggatgctgt tattttgaaa acggaattgg ctcagtatgg tcaggtcttt gtaacgacag
    16681 atgatggttc ttatggcatc aagggaaatg tttccgttgt tatcaatgat ttagatagtc
    16741 agtttgatgc tgtttactcg tgtggggctc caggaatgat gaagtatatc aatcaaacct
    16801 ttgatgatca cccaagagcc tatttatctc tggaatctcg tatggcttgt gggatgggag
    16861 cttgctatgc ctgtgttcta aaagtaccag aaagcgagac ggtcaaccaa cgcgtctgtg
    16921 aagatggtcc tgttttccgc acaggaacag ttgtattata aggagaaaat tatgactaca
    16981 aatcgattac aagtgtctct acctggtttg gatttgaaaa atccgattat tccagcatca
    17041 ggttgttttg gctttggaca agagtatgcc aagtactatg atttaaacct tttaggttct
    17101 attatgatca aggcgacaac ccttgaacca cgttttggga acccaactcc aagagtagca
    17161 gagacgcctg ctggtatgct caatgcaatt ggcttgcaaa atcctggttt agaggttgtt
    17221 ttggctgaaa agctaccttg gctggaaaga gaatatccaa atcttcctat tattgccaat
    17281 gtagctggtt tttcaaaaca agagtatgca gctgtttctc atgggatttc caaggcaact
    17341 aatgtaaaag ctatcgagct caatatttct tgtcccaatg ttgaccactg taatcatgga
    17401 cttttgattg gtcaagatcc agatttggct tatgatgtgg tgaaagcagc tgtggaagcc
    17461 tcagaagtgc cagtttatgt caaattaacc ccgagtgtga ccgatatcgt tactgtcgca
    17521 aaagctgcag aagatgcggg agcaagtggc ttgaccatga tcaatactct ggttggaatg
    17581 cgctttgacc tcaaaaccag aaaaccaatc ttggccaatg gaacaggtgg aatgtcaggt
    17641 ccagcagtct ttccagtagc cctcaaactc atccgccaag tagcccaaac aacagacctg
    17701 cctatcattg gaatgggggg agtggattcg gctgaagctg ccctagaaat gtatctggct
    17761 ggggcatctg ctatcggagt tggaacagct aactttacca atccttatgc ctgccctgac
    17821 atcatcgaaa atttaccaaa agtcatggat aaatacggta ttagcagtct ggaagaactc
    17881 cgtcaggaag taaaagagtc tctgaggtaa actgcaatca atctgttctt gattttttat
    17941 tagtttgtaa tatgaattta ggagaatttt ggtacaataa aataaataag aacagaggaa
    18001 gaaggttaat gaagaaagta agatttattt ttttagctct gctatttttc ttagctagtc
    18061 cagagggtgc aatggctagt gatggtactt ggcaaggaaa acagtatctg aaagaagatg
    18121 gcagtcaagc agcgaatgag tgggtttttg atactcatta tcaatcttgg ttctatataa
    18181 aagcagatgc taactatgct gaaaatgaat ggctaaagca aggtgacgac tatttttacc
    18241 tcaaatctgg tggctatatg gccaaatcag aatgggtaga agacaaggga gccttttatt
    18301 atcttgacca agatggaaag atgaaaagaa atgcttgggt aggaacttcc tatgttggtg
    18361 caacaggtgc caaagtaata gaagactggg tctatgattc tcaatacgat gcttggtttt
    18421 atatcaaagc agatggacag cacgcagaga aagaatggct ccaaattaaa gggaaggact
    18481 attatttcaa atccggtggt tatctactaa caagtcagtg gattaatcaa gcttatgtga
    18541 atgctagtgg tgccaaagta cagcaaggtt ggctttttga caaacaatac caatcttggt
    18601 tttacataaa agaaaatgga aactatgctg ataaagaatg gattttcgag aatggtcact
    18661 attattatct aaaatctggt ggctacatgg cagccaatga atggatttgg gataaggaat
    18721 cttggtttta tctcaaattt gatgggaaaa tagctgaaaa agaatgggtc tacgattctc
    18781 atagtcaagc ctggtactac ttcaaatccg gtggttacat gacagccaat gaatggattt
    18841 gggataagga atcttggttt tacctcaagt ctgatggcaa aatggctgaa aaagaatggg
    18901 tctacgattc tcatagtcaa gcctggtact acttcaaatc tggtggttac atggcgaaaa
    18961 atgagacagt agatggttat cagcttggaa gcgatggtaa atggcttgga ggaaaagcta
    19021 caaatgaaaa tgctgcttac tatcaagtag tgcctgttac agccaatgtt tatgattcag
    19081 atggtgaaaa gctttcctat atatcgcaag gtagtgtcgt atggctagat aaggatagaa
    19141 aaagtgatga caagcgcttg gctattacta tttctggttt gtcaggctat atgaaaacag
    19201 aagatttaca agcgctagat gctagtaagg actttatccc ttattatgag agtgatggcc
    19261 accgttttta tcactatgtg gctcagaatg ctagtatccc agtagcttct catctttctg
    19321 atatggaagt aggcaagaaa tattattcgg cagatggcct gcattttgat ggttttaagc
    19381 ttgagaatcc cttccttttc aaagatttaa cagaggctac aaactacagt gctgaagaat
    19441 tggataaggt atttagtttg ctaaacatta acaatagcct tttggagaac aagggcgcta
    19501 cttttaagga agccgaagaa cattaccata tcaatgctct ttatctcctt gcccatagtg
    19561 ccctagaaag taactgggga agaagtaaaa ttgccaaaga taagaataat ttctttggca
    19621 ttacagccta tgatacgacc ccttaccttt ctgctaagac atttgatgat gtggataagg
    19681 gaattttagg tgcaaccaag tggattaagg aaaattatat cgatagggga agaactttcc
    19741 ttggaaacaa ggcttctggt atgaatgtgg aatatgcttc agacccttat tggggcgaaa
    19801 aaattgctag tgtgatgatg aaaatcaatg agaagctagg tggcaaagat tagtactata
    19861 agtgaatatg atttgagtga atagtaagtt aaaaatcctg atttcaagta aaatcaggat
    19921 tttttcatgg atgcaatttt tttggagtct ggtgtgacgc ggagggtctt ttgtcctgtg
    19981 taagtgacaa agccgggttt tccaccagtt ggtttattga gttttttgac ttcaatcata
    20041 tctacctgca ccagattcga caggcgccct tgagagaagt aggcagctaa ctctgctgcg
    20101 tctgtcttga ctgcatcaga tgggtcaaga tttcctgaga tgacaacatg gcttccagga
    20161 atgtccttag catggaacca aagttcctcc ttgcgggcca ttttaaaggt caattcctca
    20221 ttttgaagat tgtttcgtcc gacatagatg atggttttgc catcgcttgc tagatattgt
    20281 tctagttttt tgcgtttctg gattttctcc cgttgtcttc tgcggataaa acctgtttga
    20341 atcaattctt cacggatttc agcgatttct tccagtccag cttggttgag gacggtttct
    20401 acactttcca gatagagaat agtggctttg gtttcttcaa tcaaatcagt caagtatttg
    20461 acagcttctt tgagtttctg ataccgttta aaatagcgtt gggcattctg gttgggagtc
    20521 agagccttat caagcgcaat catgataggt tggttggtat agtagttgtc taggataacc
    20581 tggtcttggt cgttaggcac ttggtggagg aaggttgtca gcaattctcc tttttgacga
    20641 aattcttcag cgttgtctgt cgccagtaac tctttttcct gttttttgag tttgtgtcgg
    20701 tttttctgaa gttcattttc aacacgacga atcagttcac tggcctgctg tttgacgcgg
    20761 tcgcgctcag ccttatcctt atagtaggtg tccaacaaat cagaaagatt tgcaagaggc
    20821 tctcccacct gatttgcaaa aggaactgga ctgaaggaag tctcagtcaa gcatggcttg
    20881 gtttcttgat tgaaaaaatt tcggaaagcg gaaagttttt cactaaccag tatcctttcc
    20941 aattcatttg ccgtatcgcg tcccagacct tgaaagaggc tttgaagatt ttttgctgtt
    21001 agttcttggg tttgcaggat ttcaaagagc ttttcatcct tgatagtaaa aggattgaga
    21061 gattttgtac ttggcggagc gatataggtc gatcctggaa gtaaggtgcg gtagctattt
    21121 tgtgaaaagc cgacgtgttt gataacttcg aggattttat gactgctttt atcgaccagt
    21181 agaatattac tgtgtttccc cataatttcg ataatcaagg tagcctggat atggtctcca
    21241 atctcgtttt tattggaaac tgtaatttcc acaatacggt cattttccac ttgctcaatc
    21301 gactcaatca gggccccctg caaatacttt ctcaaaacca tgataaaggt agaaggttgg
    21361 gctggatttt caaaagtcgt ttgggtcagc tgaatgcgtc caaaaactgg atgggcagaa
    21421 aggagcaggc gatggctttg gcaattgctg cggatttgca agaccaactc ttgttcaaaa
    21481 ggctgattga ttttctggat gcgaccattc actaattcgc ttcgcaattc ctcaactatg
    21541 tggtgtaaaa aaaatccgtc aaatgacatc gttctctcct tgtgattgta ttccatagta
    21601 ttatatcaaa aaggtagaat aaaatcatgg aaatgtggta taataaagcc aagtaaagag
    21661 aaacgagaag cacatgtata ttgaaatggt agatgaaact ggtcaagttt caaaagaaat
    21721 gttgcaacaa acccaagaaa ttttggaatt tgcagcccaa aaattaggaa aagaagacaa
    21781 ggagatggca gtcacttttg tgaccaatga gcgtagtcat gaacttaatc tggggtaccg
    21841 tgacaccgac cgtccgacag atgtcatcag ccttgagtat aaaccagaat tggaaattgc
    21901 ctttgacgaa gaggatttgc ttgaaaatcc agaattggca gagatgatgt ctgagtttga
    21961 tgcctatatt ggggaattgt tcatctctat cgataaggct catgagcagg ccgaagaata
    22021 tggtcacagc tttgagcgtg agatgggctt cttggcagta cacggctttt tacatattaa
    22081 cggctatgat cactatactc cggaagaaga agcggagatg ttcggtttac aagaagaaat
    22141 tttgacagcc tatggactca caagacaata aacgaaaatg gaaaaatcgt gacctgatat
    22201 ccagtttaga atttgccatc acagggattt ttactgctat caaggaagaa cgcaatatgc
    22261 gaaaacatgc agtgacggct ctagtggtca tccttgcagg ttttggtttt caggtgtcac
    22321 gaatcgaatg gctctttctc ctattgagca ttttcttggt ggtagccttt gaaattatca
    22381 attctgctat cgaaaatgtg gtggatttgg ccagtcacta tcacttttcc atgctggcta
    22441 aaaatgccaa ggatatggcg gctggcgcgg tattagtggt ttctcttttt gcagccttaa
    22501 caggcgcatt gatttttctc ccacgaatct gggatttatt attttaaaca gtaagaggaa
    22561 attatgactt ttaaatcagg ctttgtagcc attttaggac gtcccaatgt tgggaagtca
    22621 acctttttaa atcacgttat ggggcaaaag attgccatca tgagtgacaa ggcgcagaca
    22681 acgcgcaata aaatcatggg aatttacacg actgataagg agcaaattgt ctttatcgac
    22741 acaccaggga ttcacaaacc taaaacagct ctcggagatt tcatggttga gtctgcctac
    22801 agtacccttc gcgaagtgga cactgttctt ttcatggtgc ctgctgatga agcgcgtggt
    22861 aagggggacg atatgattat cgagcgtctc aaggctgcca aggttcctgt gattttggtg
    22921 gtgaataaaa tcgataaggt ccatccagac cagctcttgt ctcagattga tgacttccgt
    22981 aatcaaatgg actttaagga aattgttcca atctcagccc ttcagggaaa taacgtgtct
    23041 cgtctagtgg atattttgag tgaaaatctg gatgaaggtt tccaatattt cccgtctgat
    23101 caaatcacag accatccaga ccgtttctta gtttcagaaa tggttcgcga gaaagtcttg
    23161 cacctaactc gtgaagagat tccgcattct gtagcagtag ttgttgactc tatgaaacga
    23221 gacgaagaga cagacaaggt tcacatccgt gcaaccatca tggtcgagcg cgatagccaa
    23281 aaagggatta tcatcggtaa aggtggcgct atgcttaaga aaatcggtag catggcccgt
    23341 cgtgatatcg aactcatgct aggagacaag gtcttcctag aaacctgggt caaggtcaag
    23401 aaaaactggc gcgataaaaa gctagatttg gctgactttg gctataatga aagagaatac
    23461 taagtagagg tgggctcatg cctgcttctt gtttttacag aaggaggact tatgcctgaa
    23521 ttacctgagg ttgaaaccgt ttgtcgttgc ttagaaaaat tgattatagg aaagaagatt
    23581 tcgagtatag aaattcgcta ccccaagatg attaagacgg atttggaaga gtttcaaagg
    23641 gaattgccta gtcagattat cgagtcaatg ggacgtcgtg gaaaatattt gcttttttat
    23701 ctgacagaca aggttttgat ttcccatttg cggatggagg gcaagtattt ttactatcca
    23761 gaccaaggac ctgaacgcaa gcatgcccat gttttctttc attttgaaga tggtggcacg
    23821 cttgtttatg aggatgttcg caagtttgga accatggaac tcttggtgcc tgacctttta
    23881 gacgcctact ttatttctaa aaaattaggt cctgaaccaa gcgaacaaga ctttgattta
    23941 caggtctttc aatctgccct tgccaagtcc aaaaagccta tcaaatccca tctcctagac
    24001 cagaccttgg tagctggact tggcaatatc tatgtggatg aggttctctg gcgagctcag
    24061 gttcatccag ctagatcttc ccagactttg acagcagaag aagcgacagc tattcatgac
    24121 caaaccattg ctgttttgag ccaggctgtt gaaaaaggcg gttccaccat tcggacctat
    24181 accaatgcct ttggggaaga tggaagcatg caggattttc atcaggtcta tgataagact
    24241 ggtcaaggat gtgtacgctg tggtaccatc attgagaaaa ttcaactagg tggacgtgga
    24301 acccactttt gtccaaactg tcaaaggagg gactgatggg aaaaatcatc ggaatcactg
    24361 ggggaattgc ctctggtaag tcaactgtga caaattttct aagacagcaa ggctttcaag
    24421 tagtggatgc cgacgcagtc gtccaccaac tacagaaacc tggtggtcgt ctgtttgagg
    24481 ctctagtaca gcacttttgg caagaaatca ttcttgaaaa cggagaactc aatcgccctc
    24541 tcctagctag tctcatcttt tcaaatcctg atgaacgaga atggtctaag caaattcaag
    24601 gggagattat ccgtgaggaa ctggctactt tgagagaaca gttggctcag acagaagaga
    24661 ttttcttcat ggatattccc ctactttttg agcaggacta cagcgattgg tttgctgaga
    24721 cttggttggt ctatgtggac cgagatgccc aagtagaacg cttaatgaaa agggaccagt
    24781 tgtccaaaga tgaagctgag tctcgtctgg cagcccagtg gcctttagaa aaaaagaaag
    24841 atttggccag ccaggttctt gataataatg gcaatcagaa ccagcttctt aatcaagtgc
    24901 atatccttct tgagggaggt aggcaagatg acagagatta actggaagga taatctgcgc
    24961 attgcctggt ttggtaattt tctgacagga gccagtattt ctttggttgt accttttatg
    25021 cctatcttcg tggaaaatct aggtgtaggg agtgagcaag tcgcttttta tgcaggctta
    25081 gcaatttctg tctctgctat ttccgcggcg ctcttttctc ctatttgggg tattcttgct
    25141 gacaaatacg gccgaaaacc catgatgatt cgggcaggtc ttgctatgac tatcactatg
    25201 ggaggcttgg cctttgtccc aaatatctat tggttaatct ttcttcgttt actaaacggt
    25261 gtatttgcag gttttgttcc taatgcaacg gcactgatag ccagtcaggt tccaaaggag
    25321 aaatcaggct ctgccttagg tactttgtct acaggcgtag ttgcaggtac tctaactggt
    25381 ccctttattg gtggctttat cgcagaatta tttggcattc gtacagtttt cttactggtt
    25441 ggtagttttc tatttttagc tgctattttg actatttgct ttatcaagga agattttcaa
    25501 ccagtagcca aggaaaaggc tattccaaca aaggaattat ttacctcggt taaatatccc
    25561 tatcttttgc tcaatctctt tttaaccagt tttgtcatcc aattttcagc tcaatcgatt
    25621 ggccctattt tggctcttta tgtacgcgat ttagggcaga cagagaatct tctttttgtc
    25681 tctggtttga ttgtgtccag tatgggcttt tccagcatga tgagtgcagg agtcatgggc
    25741 aagctaggtg acaaggtggg caatcatcgt ctcttggttg tcgcccagtt ttattcagtc
    25801 atcatctatc tcctctgtgc caatgcctct agcccccttc aactaggact ctatcgtttc
    25861 ctctttggat tgggaaccgg tgccttgatt cccggggtta atgccctact cagcaaaatg
    25921 actcccaaag ccggcatttc gagggtcttt gccttcaatc aggtattctt ttatctggga
    25981 ggtgttgttg gtcccatggc aggttctgca gtagcaggtc aatttggcta ccatgctgtc
    26041 ttttatgcga cgagcctttg tgttgccttt agttgtctct ttaacctgat tcaatttcga
    26101 acattattaa aagtaaagga aatctagtgc gagtaaaaat taatctcaaa tgctcctctt
    26161 gtggcagtat caattaccta accagtaaaa attcaaaaac ccatccagac aagattgagg
    26221 ttttaaagta ttgtcccaag gaaagaaaag taaccctaca tcttgaatct aagtagattt
    26281 tatggtaaaa taatagggat tttaaggagt ttgatatgta taacctatta ttaaccattt
    26341 tattagtatt atctgttgtg attgtgattg caattttcat gcaaccaacc aaaaaccaat
    26401 ccagcaatgt atttgatgcc agttcaggtg atttgtttga acgtagtaaa gctcgcggtt
    26461 ttgaagctgt aatgcagcgt ttgacaggga ttttagtctt tttctggcta gccattgcct
    26521 tagcattgac ggtattatca agtagataag aaaataatgg gcaggactag gtctttgcct
    26581 ctttttattt ttaaaggatg tttgagaagg ttttacagta aaagaaaatt aaaaaatcta
    26641 gaaagaaaat atgaaagata gaataaaaga atatttacaa gacaagggaa aggtgactgt
    26701 taatgatttg gctcaggctt tgggaaaaga cagttccaag gattttcgtg agttgattaa
    26761 aaccttgtcc ttaatggaaa gaaagcacca aattcgtttt gaagaagatg gtagtctgac
    26821 attagaaatt aagaaaaaac atgagattac cctcaagggg atttttcatg cccataaaaa
    26881 tggctttggc tttgttagtc tggaaggcga ggaggacgac ctttttgtag ggaaaaatga
    26941 tgtcaactat gctattgatg gtgataccgt cgaggtagtg attaagaaag tcgctgaccg
    27001 caataaggga acagcagcag aagccaaaat tattgatatc ctagaacaca gtttgacaac
    27061 agttgtcggg caaatcgttc tggatcagga aaaacctaag tatgctggct atattcgttc
    27121 aaaaaatcag aaaatcagtc aaccgattta tgttaagaaa ccagccctaa aattagaagg
    27181 aacagaagtt ctcaaagtct ttatcgataa atacccaagc aagaaacatg atttctttgt
    27241 cgcgagtgtt ctcgatgtag tgggacactc aacggatgtc ggaattgatg ttcttgaggt
    27301 cttggaatca atggacattg tatccgagtt tccagaagct gttgttaagg aagcagaaag
    27361 tgtgcctgat gctccgtctc aaaaggatat ggaaggtcgt ctggatctaa gagatgaaat
    27421 tacctttacc attgacggtg cggatgccaa ggacttggac gatgcagtgc atatcaaggc
    27481 tctgaaaaat ggcaatctgg agcttggggt tcacatcgca gatgtttctt attatgtgac
    27541 cgaggggtct gcccttgaca aggaagccct taaccgtgcg acttctgttt acgtgacaga
    27601 ccgagtggtg ccaatgcttc cagaacgact atcaaatggc atctgctctc tcaatcccca
    27661 agttgaccgc ctgacccagt ctgctattat ggagattgat aaacatggtc gtgtggtcaa
    27721 ctataccatt acacaaacag ttatcaagac cagttttcgt atgacctata gcgatgtcaa
    27781 tgatatccta gctggcgatg aagaaaagag aaaagaatat cataaaattg tatcaagtat
    27841 cgaactcatg gccaagcttc atgaaacttt agaaaacatg cgtgtgaaac gtggagctct
    27901 caattttgat accaatgaag cgaagatttt agtggataaa caaggtaagc ctgttgatat
    27961 cgttcttcgg cagcgtggta ttgccgagcg gatgattgag tcttttatgt tgatggctaa
    28021 tgaaacagtt gccgaacatt tcagcaagtt ggatttgcct tttatctatc gaattcacga
    28081 ggagcctaag gctgaaaagg ttcagaagtt tattgattat gcttcgagtt ttggcttgcg
    28141 catttatgga actgccagtg agattagtca ggaggcactt caagacatca tgcgtgctgt
    28201 tgagggagaa ccttatgcag atgtattgtc catgatgctt cttcgctcta tgcagcaggc
    28261 tcgttattcg gagcacaatc acggccacta tggactagct gctgactatt atactcactt
    28321 taccagtcca attcgtcgtt atccagacct tcttgttcac cgtatgattc gggattacgg
    28381 ccgttctaag gaaatagcag agcattttga acaagtgatt ccagagattg cgacccagtc
    28441 ttccaaccgt gaacgtcgtg ccatagaagc tgagcgtgaa gtcgaagcca tgaaaaaggc
    28501 tgagtatatg gaagaatacg tgggtgaaga gtatgatgca gttgtatcaa gtattgtcaa
    28561 attcggtctc tttgtcgaat tgccaaacac agttgaaggc ttgattcaca tcactaatct
    28621 acctgaattt tatcatttca atgagcgtga tttgactctt cgtggagaaa aatcaggtat
    28681 cactttccga gtgggtcagc agatccgtat ccgtgttgaa agagcggata aaatgactgg
    28741 agagattgat ttttcattcg tacctagtga gtttgatgtg attgaaaaag gcttgaaaca
    28801 gtctagtcat agtggcagag ggcgtggttc aaatcgtcgt tcggataaga aggaagacaa
    28861 gagaaaatca ggacgctcaa atgataagcg taagcattca caaaaagaca agaagaaaaa
    28921 aggaaagaaa cctttttaca aggaagtagc taagaaagga gccaagcatg gcaaagggcg
    28981 agggaaaggt cgtcgcacaa aataaaaagg cacgccacga ctatacaatc gtagatacgc
    29041 tagaggcagg gatggtcctg actggaactg aaatcaagag tgtacgagct gctcgaatta
    29101 atctcaagga tggctttgct caagtgaaaa atggagaagt ttggctgagc aatgttcata
    29161 tcgcgcctta cgaagagggc aatatctgga accaggaacc agaacgtcgt cgtaaactcc
    29221 tactccataa aaagcaaatt caaaaattgg aacaagagac caaagggaca ggaatgacct
    29281 tagttcccct taaggtctat ataaaagatg gctacgctaa gcttctttta ggacttgcca
    29341 aagggaagca tgactatgac aaacgggagt ctatcaaacg tcgtgagcaa aatcgagata
    29401 tcgcgcgtgt gatgaaagct gttaatcagc gataaaaaga ggaattgaaa atggaaaaat
    29461 tagttgccta taaacgcatg cctttgtgga ataaacaaac aatgcctgaa gatgttcagc
    29521 aaaagcacaa tacaaaagtt gggacttggg ggaaaattac tgtcttgaag ggagctctca
    29581 agtttattga attgacagaa gaaggggaag ttctagctga acacctcttt gaagcagggg
    29641 cagacaatcc aatggcccaa cctcaagcct ggcaccgagt ggaagctgcc acagatgatg
    29701 tggaatggta cttggaattt tattgtaaac ctgaggatta ttttgctaaa aaatacaata
    29761 ccaatcctgt tcattcagag gtcctagagg ccatgcagac agtgaaacaa gggaaagctt
    29821 tggatttggg ttgtggtcag gggcgtaatt ctctttttct agcccagcaa gattttgatg
    29881 tgacggctgt agatcaaaat ggactagctc ttgaaatctt gcaaagcatt gtggagcagg
    29941 aagatttgga catgcctgtt ggcctttacg atatcaattc agctagcatt gaacaagaat
    30001 atgattttat cgtttcaaca gttgttctca tgtttctaca agcggaccgc attccagcta
    30061 ttattcaaaa tatgcaggag aaaaccagtg ttggtggtta caaccttatc gtttgtgcca
    30121 tggacacgga ggattatcct tgctcggtta atttcccatt cacctttaaa gaaggagaac
    30181 tggcagacta ttacaaggat tgggaattgg ttaagtacaa tgaaaatcca ggccatttgc
    30241 accgtcgcga tgagaatggc aatcgtattc aactacgctt tgcgacctta ctagctaaga
    30301 aaatcaagta aacacacatg aagattagga attttcctga tcttttttct tttttacgaa
    30361 tgatatagaa aaggagggaa ttcatgtttg ttgcgagaga tgctagggga gaattggtaa
    30421 atgtgttaga ggataaactt gagaagcaag catacacctg cccagcttgt ggaggccagc
    30481 tccatttgcg tcaaggacca agtgtacgga cgcattttgc ccataaatcc ttaaaagact
    30541 gtgatttttt ctttgaaaat gaaagtccag aacacctggc caataaggaa tccctctatc
    30601 actggttgaa aaaagagaca aaggttcaat tagagtaccc gctttcagaa cttaaacaga
    30661 ttgcggatgt atttgtaaat ggcaatctag ctctagaagt tcagtgtagt cccttgcctc
    30721 agaaagtcct taaagagcga agtgagggat atcgtagtca gggttaccaa gtactgtggt
    30781 tgctgggtca aaaactgtgg ctcaaggagc gtttgactcg tctacagcaa ggttttcttt
    30841 atttcagtca aaacatgggc ttttatgttt gggaattaga caaggaaaaa caagttttaa
    30901 gactcaaata cctgatttac caggatctcc gcggtaaact ccattatcaa atcaaggaat
    30961 tttcctatgg tcaaggtagt ttattggaaa tattgcgtct tccctataag aaacaaaaaa
    31021 tatctcattt tacagtttct gaggacaagg acatctgtcg ctatatccgg caacaacttt
    31081 attatcaaaa tctcttttgg atgaaagaac aagcagaagc ctatcaaaag ggagaaaata
    31141 tcctgactta tggactgaaa gaatggtatc cacaaattcg accaatagtg ggcaaatttt
    31201 tccagattga acaagacttg actagctatt atcagcactt ttatacctat taccaaaaaa
    31261 atcctcaaaa tgattggcaa aagctttatc caccagcctt ttatcagcaa tatttcttga
    31321 aaaatatggt agaatagaaa ggatggagga atctaatggt attacaaaga aatgaaataa
    31381 atgaaaaaga tacatgggat ctatcaacga tctacccaac tgaccaggct tgggaagaag
    31441 tcttaaaaga tttaacagaa caattggaga cagtagccca gtatgaaggc catctcttgg
    31501 atagtgcgga taacctacta gaaatcactg aattttctct tgaaatggaa cgccagatgg
    31561 agaagcttta cgtttatgct catatgaaga atgaccagga tacacgtgaa gctaagtatc
    31621 aagagtacta tgccaaggcc atgacattct acagccagtt agaccaagcc ttttcattct
    31681 atgagcctga atttatggag attagcgaaa agcagtatgc tgacttttta gaagctcaac
    31741 caaagctgca ggtttatcaa cactattttg acaagctctt gcaaggcaag gatcacgttc
    31801 tctcacaacg tgaagaagaa ttattggctg gagctggaga aatctttggt tcagcaagtg
    31861 aaaccttcgc tatcttggac aatgcggata ttgtgttccc ttatgtccta gacgatgatg
    31921 gtaaagaagt tcagctatct catgggattt acacacgttt gatggagtct aaaaaacgtg
    31981 aggttcgccg tggtgcctat caagctcttt atgcgactta cgaacaattc caacacacct
    32041 atgccaaaac cttgcaaacc aatgttaagg tgcaaaatta ccgtgctaaa gttcgtaact
    32101 acaagagtgc tcgtcatgca gccctcgcag cgaattttgt tccagaaagt gtttatgaca
    32161 atttggtagc agcagttcgc aagcatttgc cactcttaca tcgctatctt gagctttgtt
    32221 caaaaatctt ggggatttca gatctcaaga tgtacgatgt ctacacaccg ctttcatctg
    32281 ttgaatacag ttttacctac caagaagcct tgaaaaaagc agaagatgct ttggcagtct
    32341 tgggtgagga ttacttgagc cgtgttaaac gtgccttcag cgagcgttgg attgatgttt
    32401 acgaaaatca aggcaagcgt tcaggtgcct actctggtgg ttcttacgat accaatgcct
    32461 ttatgcttct caactggcag gacaatctgg acaatctctt tactcttgtt catgaaacag
    32521 gtcacagtat gcattcaagc tatactcgtg aaactcagcc ttatgtttac ggagattact
    32581 ctatcttttt ggctgagatt gcctcaacta ccaatgaaaa tatcttgacg gagaaattat
    32641 tggaagaagt ggaagacgac gcaacacgct ttgctattct caataacttc ctagatggtt
    32701 tccgtggaac agttttccgc caaactcaat ttgctgagtt tgaacacgcc attcaccaag
    32761 cagatcaaaa tggggaggtc ttgacaagcg atttcctaaa taaactctac gcagacttga
    32821 accaagagta ttatggtttg agtaaggaag acaatcctga aatccaatac gagtgggctc
    32881 gcattccaca cttctactat aactactatg tatatcaata ttcaactggc tttgcggccg
    32941 cctcagcctt ggctgaaaaa attgtccatg gtagtcaaga agaccgtgac cgctatatcg
    33001 actacctcaa ggcaggtaag tcggactatc cacttaatgt catgagaaaa gctggtgttg
    33061 atatggagaa ggaagactac ctcaacgatg cctttgcagt ctttgaacgc cgtttaaatg
    33121 agtttgaagc ccttgttgaa aaattaggat tggcataaaa tggttgaatc gtatagtaag
    33181 aatgctaacc ataacatgcg tcgtcctgtc gtcaaagaag aaattgtaga cttgatgcgt
    33241 cagcgtcaaa agcaggtcac aggtttcttg aaagaattgg aaaactttgc ccgcaaggaa
    33301 aatattccta ttattcccca tgaaacggtt gcttatttcc gttttcttat ggaaaccatg
    33361 cagcctaaaa atattctgga aattgggacg gctatcggtt tttcagctct cttgatggct
    33421 gaacatgcgc caaatgctaa gattacaact attgatcgta atccagaaat gattggtttt
    33481 gccaaggaaa attttgccca gtttgacagt cgcaagcaaa tcactctcct agagggagat
    33541 gcggtggatg tcttatctac actgacagag tcttatgatt tcgtctttat ggattctgcc
    33601 aagtctaaat acatcgtctt tctgccagaa atcctcaaac atttggaagt tggtggtgtg
    33661 gttgtcttgg atgatatttt tcaaggtggt gatgttgcca aggatattat ggaagtccgt
    33721 cgtggtcagc gaaccattta tcgaggcctt caaaaattat ttgatgcaac cttagacaat
    33781 ccagaactca ccgcaacatt agtgccttta ggagatggta ttctcatgct tcgtaaaaat
    33841 gtagcagatg ttcaactgtc tgaaagcgaa tgattttcag aaaaatttaa gaaaaaatag
    33901 taaaatagat agagtaacac ttatctcaaa ggagtagaca tgaagaaaaa attattggca
    33961 ggtgccatca cactattatc agtagcaact ttagcagctt gttcgaaagg gtcagaaggg
    34021 gcagacctta tcagcatgaa aggggatgtc atcacagaac atcaatttta tgagcaagtg
    34081 aaaaacaacc cttcagccca acaagtcttg ttaaatatga ccatccaaaa agtttttgaa
    34141 aaacaatatg gctcagagct tgatgataaa gaggttgatg atactattgc cgaagaaaaa
    34201 aaacaatatg gcgaaaacta ccaacgtgtc ttgtcacaag caggtatgac tcttgaaaca
    34261 cgtaaagctc aaattcgtac aagtaaatta gttgagttgg cagttaagaa ggtagcagaa
    34321 gctgaattga cagatgaagc ctataagaaa gcctttgatg agtacactcc agatgtaacg
    34381 gctcaaatca tccgtcttaa taatgaagat aaggccaaag aagttctcga aaaagccaag
    34441 gcagaaggtg ctgattttgc tcaattagcc aaagataatt caactgatga aaaaacaaaa
    34501 gaaaatggtg gagaaattac ctttgattct gcttcaacag aagtacctga gcaagtcaaa
    34561 aaagccgctt tcgctttaga tgtggatggt gtttctgatg tgattacagc aactggcaca
    34621 caagcctaca gtagccaata ttacattgta aaactcacta agaaaacaga aaaatcatct
    34681 aatattgatg actacaaaga aaaattaaaa actgttatct tgactcaaaa acaaaatgat
    34741 tcaacatttg ttcaaagcat tatcggaaaa gaattgcaag cagccaatat caaggttaag
    34801 gaccaagcct tccaaaatat ctttacccaa tatatcggtg gtggagattc aagctcaagc
    34861 agtagtacat caaacgaata gtccaaatca atgagtcagg gaaaaaaact cgacttcagg
    34921 aaaaaatgaa gcaaacattc ccacaataaa acgcatagta caaggtttgt actgacccca
    34981 aaagttagac aattaattta tctgaaggat ttagttctgt attgcacagg gctaagtcct
    35041 tttagtttta ccttaattct cttattgttg taataatcaa tatagtctat aatggcttgt
    35101 tccaattgat taagtgattt aaatgttttc tcatagccat aaaacatttc ggattttaaa
    35161 atgccaaaga aagattccat cctaccgttg tcttggctgt ttcctttgcg tgacatagat
    35221 gcttgaattc ccttactctc taggaagcga tgataagaat cgtgttggta ttgccagcct
    35281 tggtcactat ggagaatcgt attctcgtag tgcttctctt tgaatgcctg ttccaacatt
    35341 aacgatcaat caatttaatc atgtacctaa tattagaatt gtttatccca aatttatttg
    35401 aaagcttctc taagctatat ccttgttttc taagttcata gatctgaact ttatcatcat
    35461 aagttaattt cataataaaa acaccccaaa agttagattt tttctgtcta acttttgggg
    35521 tgtagttcat gtacacctga tatgatgcgt tttataattt taaagacttt ttgaccagcc
    35581 tcattttttt aacttgatac tcagtgaaaa gcaaagatta aactaggaag ctagctgtag
    35641 gctgctcaaa gaacagcttt gaggttgcag ataaaacttg tgaggtcacc aacatatata
    35701 atgtgaagct gacgtggctt gaatagattt tagaagggta tgagtctgga agttttaatg
    35761 gataatgcaa gattccatag aatgggtaag ctagagttct tatgtgaaga gtttgggcat
    35821 aaacttttac cttttcctcc ctactcatct tagtatagaa aagtgaatct aaaatagtac
    35881 ataactgctt ctaaaacatt cttataaatt gatttaaatt ctcaaatcat attattcagt
    35941 tcttatttca ttttgctcta caatcctgtt gagaagacac gtgttcatat caaaaaggta
    36001 ttggcaagtt gcaatacctt tttacaaggt tcttttgtct tatttttgtt tcaactgact
    36061 atatctccta tggttctagt tcagaaggct aggctataat tatgattgat aagaagtatc
    36121 attccaagta ttgagagtga atgtttcaaa atcatgggtt tctataatgg tcaggctggc
    36181 atttgctaga ccgccatctt tacgaagaag tggttcttta tagcctagga gagtacgaag
    36241 actggcagta agattggcgc cgtgtccgac aattagaata cgctcagctg gactatcttt
    36301 taatgatttg ataaattgga tggtccgttg agttgtacta tagagggatt cggctccgaa
    36361 cattcgagtg tcaaattgag caagatttga acgaaaagcc tggatttgtt gcgggtaaat
    36421 agcttccaag gttgcaattt tcaaaccttc taacttccca agttgccatt cacggagatt
    36481 aggaacgatt tctaaagaac agggggtata gagttgactt tggataatct cagcagattt
    36541 gaccgctcga ggtaaatcac ttgaataaat ctgatcaaaa ggaatttcct tgagatactg
    36601 gccaagtcgt tttagggttt caatggattc aggaagaaga ggagaatcac cgctagcatc
    36661 ttgaaaacga ccttcttggt tccagagggt acgaccgtgg cggacaaagt agagtttcat
    36721 tacttgatgt cctccaaaat atctacaaag tctgccttta caaagctagc caagtcttgt
    36781 ggcgcgacga taatgctgtg tccgacttcg cctgcagaga caatcattcg atccaaatct
    36841 agagcaattt tatcgataaa aatgggataa ttgtgtttct gacgaattcc gacaggatta
    36901 ttggctccat gaatgtaacc agttgttttt tctaagtcct tttgtggaat catgctcact
    36961 tttttattgc cagaaatttt agctagtttc ttttcagaca agtgctgagt gatagggaca
    37021 attccgataa tcggtccggt cttgtctccc aaaagcgcca aggttttgaa aatctgatct
    37081 cgttcataac cttgaggaag ctctccttct agggcattga tttgaatccc ctgatgaggg
    37141 atagctgctt tagataggat ttgttccacc aatgtttttt tgattttaac tttttttgcc
    37201 attatttata tttatcctcc aattgactca tccaaatacc aagccagatt cccagcgcaa
    37261 agaagaaggc gatgatgaca taaccgacaa gtgaaagtcc tgtgtattgg atactttcag
    37321 cgtttcctgc atttggaatt aagatcaaaa gggtacttga taggacgata ccgatgatga
    37381 aatgatagac gcgtgagtgg tagttgttta aggcataatc catcaatttt gaaaaaacga
    37441 tgagagttgc acctgcacca attccaatcg gaaagaaggt tcccaagaga tcaaaagttt
    37501 taaaaccagt caacatagga gcatagagtc ccaaaatcaa aagtaagttc gatgggctga
    37561 ggccaggaac caagacgcca agggccaata gtgcgcctgc taggacgaag ttaagaaagc
    37621 tggcgcttaa ggttccaacg acaaaattta aggcatagag tcctaatcca gaaatgataa
    37681 aggttgtcca taaccaagct aaatcaatct tgtctcggtc agattctcga gttgattctt
    37741 tgaggaggct aggaactgta ccgataatag ctcccgcaaa gctccataat acaaagacct
    37801 gataattttc aagcaggtat tcaatcgggt aggaaaataa gccgattccc agaagcatac
    37861 cgatggcaac tggaataaag tacaaaacat tttctttaag gtctttaaag ggatgggcca
    37921 gaaagccaat cattcgttca tagattccta agattgctgc tagaacccct ccggaaattc
    37981 ccggtaggat aaatccaaga gcaattacaa tccctttaat aacgcgtgct aaccatgaga
    38041 gcatatattt cctccaacta tttctatttc aaaaattcct tactatattg tatcacaatc
    38101 agacacaaaa agaaaaagca aatgataaac aaatgctttt aaggttttaa aaaaagtttt
    38161 cgaaaggttt cttctttatt ttttaaggga gagataacgt tgatatctaa atcgtggtca
    38221 aagccggcaa tttttccttt agatgtgtat tggtgaatat cataatctaa atcagtttta
    38281 ggactgctct ccaaaaatcc tgagtctgag ccgtgggacg gaatccaaac agaggtaaac
    38341 ttgcctgtat caatactgtg ttcttccatg aagtagacac caacgtagat gccgatgttt
    38401 ttagcaccta gtgatgctag ttttgctcga aagttttcga caccttcgtt catattagac
    38461 atggttttgt cttccacgtc aagccaatag taactagggc tgtaaggaga ggcagcattg
    38521 tagaaaactt cggcagcctt ttccatttct tggacacttt ttccagctac ataagcgtag
    38581 acagcaactg ggacattccg cttttgaagt tcagtgatat gactcttata ggccttgtct
    38641 attccattga taaatgaagc atcattttct tttgttgttt gagcaccact gtgaacacga
    38701 acaatagcac ctgaaatatt ttgtgagagg gcatcgtagt tgatttcctc aggacgctgc
    38761 cagccagaga ggtcaataat cggtttgtct aagtgtttca aagcctgtgc ttcaatctgt
    38821 gctatattgg attttgtttt aaacgattgg ctgtcattaa gtgggcgatt gatgattaaa
    38881 atgaacatca taatcccaaa aaaactaaat aaaataagtg gatgaatttg ttttctcata
    38941 tcttataagt ctaccctaaa aatcaaaaaa aatcaaaaaa atgggttaag gaagagactt
    39001 tagagcattt tttcattcaa gagtgcggaa tgatttgaaa tatggtataa taaaagggaa
    39061 tttctacaga aaagagaaga ttatgtcaaa ttttgccatt attttagcag cgggtaaagg
    39121 gactcgcatg aaatctgatt tgccaaaagt tttgcacaag gttgcgggta tttctatgtt
    39181 ggaacatgtt ttccgtagtg tgggagctat ccaacctgaa aagacagtaa cagttgtagg
    39241 acacaaggca gaattggttg aggaggtctt ggctggacag acagaatttg tgactcaatc
    39301 tgaacagttg ggaactggtc atgcagttat gatgacagaa cctatcttag aaggtttgtc
    39361 aggacacacc ttggtcattg caggagatac tcctttaatc actggtgaaa gcttgaaaaa
    39421 cttgattgat ttccatatca atcataaaaa tgtggccact atcttgactg ctgaaacgga
    39481 taatcctttt ggctatggac gaattgttcg taatgacaat gctgaggttc ttcgtattgt
    39541 tgagcagaag gatgctacag attttgaaaa gcaaatcaag gaaatcaaca ctggaacata
    39601 cgtctttgac aacgagcgtt tgtttgaggc tttgaaaaat atcaatacca ataacgctca
    39661 aggcgaatac tatattacag acgtcattgg tattttccgt gaaactggtg aaaaagttgg
    39721 cgcttatact ttgaaagatt ttgatgaaag tcttggggta aatgaccgtg tggcgcttgc
    39781 gacagctgag tcagttatgc gtcgtcgcat caatcataaa cacatggtca acggtgttag
    39841 ctttgtcaat ccagaagcaa cttatatcga tattgatgtt gagattgctc cggaagttca
    39901 aatcgaagcc aatgttacct tgaaagggca aacgaaaatt ggtgctgaga ctgttttgac
    39961 aaacggtact tatgtagtgg acagcactat cggagcagga gcggtcatta ccaattctat
    40021 gattgaggaa agtagtgttg cagacggtgt gacagtcggt ccttatgctc acattcgtcc
    40081 aaattcaagt ctgggtgccc aagttcatat tggtaacttt gttgaggtga aaggatcttc
    40141 aatcggtgag aataccaagg ctggtcattt gacttatatc ggaaactgtg aagtgggaag
    40201 caacgttaat ttcggtgctg gaactattac agtcaactat gacggcaaaa acaaatacaa
    40261 gacagtcatt ggagacaatg tctttgttgg ttcaaattca accattattg caccagtaga
    40321 acttggtgac aattccctcg ttggtgctgg ttcaactatt actaaagacg tgccagcaga
    40381 tgctattgct attggtcgcg gtcgtcagat caataaagac gaatatgcaa cacgtcttcc
    40441 tcatcatcct aagaaccagt aggagcctat catggagttt gaagaaaaaa cgcttagccg
    40501 aaaagaaatc tatcaaggac caatatttaa actggtccaa gatcaggttg aattaccaga
    40561 aggcaaggga actgcccgac gggatttgat tttccacaat ggggctgtct gtgttttagc
    40621 agtaacggat gaacaaaaac ttatcttggt caagcagtac cgcaaagcta tcgaggctgt
    40681 ctcttacgaa attccagccg gaaaattgga agtaggagaa aacacagccc ctgtggcagc
    40741 tgcccttcgt gaattagagg aagaaacagc ctatacaggg aaattagaac tcttgtacga
    40801 tttttattca gctattggct tttgtaatga gaagttaaaa ctatatttag caagcgattt
    40861 gacaaaagtg gaaaatccgc gtccgcagga tgaggatgaa accttggaag tccttgaagt
    40921 gagcttagaa gaagcgaaag aattaatcca atcaggtcat atctgtgatg ccaagacaat
    40981 tatggctgtt cagtattggg agttgcagaa aaaatagagg aggtcagtat gggtaaatct
    41041 ttattaacgg atgaaatgat tgaaagagct aatagaggcg aaaaaatttc aggtcctcct
    41101 ttgctagatg ataatgagga aactaagatt ttaccaacct cttcttcccg ttttggttat
    41161 gccaatccta aggatcatgg ttttagccag gaaaccttga agattcaggt cgaaccatct
    41221 attcataaaa gccgtcgtat tgaaaatacc aagagaaatg tcttcaattc taagttgaat
    41281 aaaatcttat ttgcggtcat ctttctcttg attttgcttg ttttagcaat gaaacttttg
    41341 taatagaaaa ggaattgaaa tgaaaatagg aattattgct gctatgccag aagaactggc
    41401 ttatctggtc cagcatttag ataataccca ggagcaagtt gttttgggga atacctatca
    41461 tacaggaacc attgcttctc atgaagtcgt tcttgtagaa agtggaattg gtaaggtcat
    41521 gtctgctatg agtgtggcga ttttggctga tcatttccag gtggatgccc ttattaatac
    41581 gggttcagct ggggcagtag cagaaggtat cgctgttggg gatgtcgtga ttgctgacaa
    41641 attagcctat catgacgtgg atgtcacagc ttttggctat gcttatggac aaatggcgca
    41701 acaaccgctt tatttcgaat cagacaaaac ctttgttgct caaatccaag agagtttatc
    41761 tcaattggac caaaactggc atcttggttt gattgctaca ggagatagtt ttgttgcagg
    41821 aaatgacaag atagaagcga ttaagtccca tttcccagaa gttttagccg tggagatgga
    41881 gggggcagct attgctcaag cagcgcatgc cctcaatctc ccagtcttag tcatcagagc
    41941 tatgagtgac aatgccaacc atgaagcaaa catctttttt gatgagttta ttatcgaagc
    42001 tggacgtcgc tctgcccaag tcttgttgac ctttttgaag gctttagatt aagcggaaat
    42061 ttgacagttt ttctagctta tgataagatt taagtaaaga aaagctagaa aacgtttcag
    42121 aggatattat gagtattgaa atgaccgtca gtgagattgc agaggtctta ggattatctc
    42181 gccaagcaat caataaccgt gtcaaagaat taccagaaga agacacagat aaaaatgaca
    42241 aaggggtaac agttgttacc agaagtggct tgattaagct agaagaaatc tataaaaaaa
    42301 cgatttttga agatgagcct gtcagtgaag atgtcaaaca acgtgaactg atggagattc
    42361 tagtggatga gaagaatgca gaaattcttc gtctttatga acaattaaaa gccaaggatc
    42421 gtcagttatc agaaaaagac gagcagatgc gtatcaaaga ccgtcagatt gctgagaaag
    42481 acaaacaatt agatcagcaa caacaattga cccttcaagc catgaaggat caagaaaatc
    42541 ttaagctaga gctggaccaa gcaaaagaag aagtccaatc cactaagaaa ggcttttttg
    42601 ctcgtttatt tggaggataa tcaaggagct gtttaggtta aatgctaacc tgatggcttc
    42661 ttttgtttgt gaatagtata gttgattaga acagagacag tagaggagag gtacagaatg
    42721 gaacgattag aagattacat tgcttttgac ttagaattta atcagcacga aggacaaaca
    42781 cacttgattc aagtatcggc agtgcgtttt agagatggtc gggaaatcga tgcctatgat
    42841 tcctatgttc ataccagcgt ccctctaaaa agctttatca atggtttgac cgggattaca
    42901 gctgagacct taaaagatgc tcctccagtt gagaaagtct tagaagaatt ccaagagttt
    42961 gtgggttctt tgctgatgat aggctacaat gctgctaaaa gtgatttacc tattttagca
    43021 gagcatggtt tagactatag ccaacagtac aaggtggatt tgtatgatga agcttttgaa
    43081 cgtcggagtt cagatttaca tggtattgcc aatctcaaat tgcaaactgt tgcctcattt
    43141 cttggatttc aaggtcagtc tcataatagt ttagaggatg ctcggatgac ggcgcgtgtt
    43201 tacgaatcct tcttagagtc ggatcaagct agggaattgt tagagacaga aagtagctta
    43261 tcaaacaatc cttttggtgt cttggacttg tctcaattat ttgactagga gtgcctatga
    43321 aacctgaaaa acctaaaaaa ttgggtctta aacagattta cctcaatctt gataaaatcc
    43381 tctttctatt tttcttgatt tttatgatag tggagtattt gtgggtacca ttcaattcat
    43441 ggttggctag cttcttatta gatcagacag gttatctttt tatttcctat aacaatgtct
    43501 tagccatcct tactcattct ccattgatta gtctagcttt tttagccttg attgtaatta
    43561 accttctggt tgcttatttt caaataggac tcctctttat tggtgctcgt catctcctct
    43621 ataatgaaaa gcgaaggcta attgagtata tccgcaaggt tttccatgaa agttttggat
    43681 ttatgaaaaa gctaacttgc gctaaagcct tgtttatctt tttctatata gctatgcttt
    43741 ttccttttat acggaaaatg ctgaagattt attatcttaa taaaatcgtc attccagagt
    43801 ttatccaagc ttatttagaa gataaatatt ggatgtggtg gctgagcatc ctacttctat
    43861 ctcttatctt tctctatgtc tctgtcagat tgatgtttgc ccttccgaag attgtatatg
    43921 atcagctgac tgttcgagaa gcagtgatgt ttagcttgga gaaaaccaag aagagagtta
    43981 ctttttatgc ttggaattta ttttacatct tgctcaaagc acatttatta ttttatcttc
    44041 ctttgattcc tttattattg gctcaaactt tggtagatga tatcactcag agagagtctc
    44101 tgattcttgg tattttgaat tttgtcgtga ttaaaaatct ctattatatg gctctgactt
    44161 attttttggt taaatttgtt tcatttttga caggaaagga gctggatatg cttcctagga
    44221 gagaaaaaga tcacatcgtg cgatggggtg tcatgacttg tgccagtctt ttctttgcct
    44281 tagaaggtta tatttatctg gaggctccca tggttcatct acctcaactt atttctcacc
    44341 gaggggtttc caatgcaaat gggattcaaa atacagtaga gtccttggaa actacagcac
    44401 aactcaaacc agacttggtg gagacggacg tgcaggaaac aaaagatggg cagtttgtca
    44461 tgatgcatga tgctaacttg aaaaatctag caggtatcaa taaaagtcct caagacttaa
    44521 acttggagga gcttaaaggg attgatattt ttgaaaatgg ctaccagact aaaatttcaa
    44581 gctttgaaga ttatctcagt cgagccaacg aacttggtca aaaattacta attgaaatta
    44641 aaaccagtaa aaaagatagt ccagacatga tgaaccgctt tttagcccgt tatgctgcaa
    44701 agctcaagat ttatggacat caaatccagt ctttagacta ccatgttgtc gaaaaagtaa
    44761 gacagtatga tgccgaactg ccagtttatt tcatcatgcc ctacaattct gtctttccta
    44821 aaacaagagc gacaggatat actatggagt actcaacctt ggatgaatat tttgtaagca
    44881 aactatggac aacggatcag aaactttatg tctggactgt caatgattca gaagctatca
    44941 gcaaatctct tcacttagga gtagatggag tgattacaga tgatttggaa aaagttcagc
    45001 gggaaataga agtagcccaa gaagatccag aatatacgga tttgctcttg aaaaaagcat
    45061 tggaattctt tgaattttag taataaaaaa gaggaacggt tgttcctccc atgataggta
    45121 agtattcttg tgaatgcttg cttttttgtg gttgcaatcc gtttacgaga attgtttgta
    45181 ttgttcttgt ttcaatttac tttatagtgc tttgaagttg gaatgctaca ctataacttc
    45241 t
//
LOCUS       DAVBGY010000012        39918 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVBGY010000012 DAVBGY010000000
VERSION     DAVBGY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39918
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.012"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            655..2808
                     /locus_tag="VQ620_000671"
     CDS             655..2808
                     /locus_tag="VQ620_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HET2763343.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2821..4170
                     /locus_tag="VQ620_000672"
     CDS             2821..4170
                     /locus_tag="VQ620_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET2763344.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAMETGASLASQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4340..5047
                     /locus_tag="VQ620_000673"
     CDS             4340..5047
                     /locus_tag="VQ620_000673"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET2763345.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            5249..8974
                     /locus_tag="VQ620_000674"
     CDS             5249..8974
                     /locus_tag="VQ620_000674"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET2763346.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTVFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPLVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9067..10509
                     /locus_tag="VQ620_000675"
     CDS             9067..10509
                     /locus_tag="VQ620_000675"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET2763347.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSMTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10546..11568
                     /locus_tag="VQ620_000676"
     CDS             10546..11568
                     /locus_tag="VQ620_000676"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET2763348.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLVSSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11565..12110
                     /gene="purN"
                     /locus_tag="VQ620_000677"
     CDS             11565..12110
                     /gene="purN"
                     /locus_tag="VQ620_000677"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET2763349.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12194..12703
                     /locus_tag="VQ620_000678"
     CDS             12194..12703
                     /locus_tag="VQ620_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET2763350.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12728..14275
                     /gene="purH"
                     /locus_tag="VQ620_000679"
     CDS             12728..14275
                     /gene="purH"
                     /locus_tag="VQ620_000679"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET2763351.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAANENQIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYTVVLDELSAN
                     GETTYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIAVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14397..15659
                     /gene="purD"
                     /locus_tag="VQ620_000680"
     CDS             14397..15659
                     /gene="purD"
                     /locus_tag="VQ620_000680"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET2763352.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTATYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            16062..16550
                     /gene="purE"
                     /locus_tag="VQ620_000681"
     CDS             16062..16550
                     /gene="purE"
                     /locus_tag="VQ620_000681"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET2763353.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16537..17628
                     /gene="purK"
                     /locus_tag="VQ620_000682"
     CDS             16537..17628
                     /gene="purK"
                     /locus_tag="VQ620_000682"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET2763354.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPVSRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSQDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVTENPSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVEEFGEGIDF"
     gene            17638..17865
                     /locus_tag="VQ620_000683"
     CDS             17638..17865
                     /locus_tag="VQ620_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HET2763355.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            17928..19226
                     /gene="purB"
                     /locus_tag="VQ620_000684"
     CDS             17928..19226
                     /gene="purB"
                     /locus_tag="VQ620_000684"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET2763356.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCNKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19281..23219)
                     /gene="strH"
                     /locus_tag="VQ620_000685"
                     /pseudo
     CDS             complement(19281..23219)
                     /gene="strH"
                     /locus_tag="VQ620_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
     gene            complement(23534..24250)
                     /locus_tag="VQ620_000686"
     CDS             complement(23534..24250)
                     /locus_tag="VQ620_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2763357.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            24598..26385
                     /locus_tag="VQ620_000687"
     CDS             24598..26385
                     /locus_tag="VQ620_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET2763358.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            26382..26858
                     /locus_tag="VQ620_000688"
     CDS             26382..26858
                     /locus_tag="VQ620_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET2763359.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            26886..27791
                     /locus_tag="VQ620_000689"
     CDS             26886..27791
                     /locus_tag="VQ620_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2763360.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            27778..28593
                     /locus_tag="VQ620_000690"
     CDS             27778..28593
                     /locus_tag="VQ620_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2763361.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            28600..29004
                     /locus_tag="VQ620_000691"
     CDS             28600..29004
                     /locus_tag="VQ620_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2763362.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            29257..30423
                     /locus_tag="VQ620_000692"
     CDS             29257..30423
                     /locus_tag="VQ620_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET2763363.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVAKPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKV
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVVLGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            30539..31576
                     /locus_tag="VQ620_000693"
     CDS             30539..31576
                     /locus_tag="VQ620_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HET2763364.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            31783..32637
                     /locus_tag="VQ620_000694"
     CDS             31783..32637
                     /locus_tag="VQ620_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2763365.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKEELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAG"
     gene            32787..33044
                     /locus_tag="VQ620_000695"
     CDS             32787..33044
                     /locus_tag="VQ620_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET2763366.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(33419..33511)
                     /locus_tag="VQ620_000696"
                     /pseudo
     CDS             complement(33419..33511)
                     /locus_tag="VQ620_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(33547..33843)
                     /locus_tag="VQ620_000697"
     CDS             complement(33547..33843)
                     /locus_tag="VQ620_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2763367.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLISQGLTVTNVEGGMNAWPGQVE"
     gene            complement(33833..33994)
                     /locus_tag="VQ620_000698"
     CDS             complement(33833..33994)
                     /locus_tag="VQ620_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HET2763368.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            34064..34828
                     /locus_tag="VQ620_000699"
     CDS             34064..34828
                     /locus_tag="VQ620_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HET2763369.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLDEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            complement(35107..35268)
                     /locus_tag="VQ620_000700"
     CDS             complement(35107..35268)
                     /locus_tag="VQ620_000700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763370.1"
                     /translation="MRTKASIRKLVTDCSKHCFEVANRTDEVSSKHCFEVANRTDEVS
                     SKHRFEVAG"
     gene            35486..36865
                     /locus_tag="VQ620_000701"
     CDS             35486..36865
                     /locus_tag="VQ620_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET2763371.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            36879..37544
                     /locus_tag="VQ620_000702"
     CDS             36879..37544
                     /locus_tag="VQ620_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET2763372.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            37679..37837
                     /locus_tag="VQ620_000703"
     CDS             37679..37837
                     /locus_tag="VQ620_000703"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763373.1"
                     /translation="MKIKDQTRKLATGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTARRR"
     gene            37899..38438
                     /locus_tag="VQ620_000704"
     CDS             37899..38438
                     /locus_tag="VQ620_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2763374.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLVEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTDLLRASQF"
     gene            38505..38915
                     /locus_tag="VQ620_000705"
     CDS             38505..38915
                     /locus_tag="VQ620_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HET2763375.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLIDQLD
                     LSQVSHPKMREYLLNHIELTTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            39031..>39918
                     /gene="pavB"
                     /locus_tag="VQ620_000706"
     CDS             39031..>39918
                     /gene="pavB"
                     /locus_tag="VQ620_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HET2763376.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADG
                     LNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     SPSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLV"
BASE COUNT        11003 a         7757 c         9343 g        11815 t
ORIGIN      
        1 atgatttaat tagaaaaaaa tttttaaagt cttcggagaa gaaaacagaa aaatctgtta
       61 aaaaataatc aaaactataa atgatgaatc tgaatcaaaa ttattttgcg catgtaaaga
      121 ggagtcttat agtaacgagt caaaaaagga gtaactatga atcgtaattt agaacggtgt
      181 tatctattct gactaggaat agatcatacc agaggtagct tagaaatagc agggacatta
      241 gaaattgaag taataaatag gatgtcgtaa gtgttactat caatgattta tttgtttcaa
      301 gcttgcctag ggtgacagta aaaaatcaat ttcctttcaa tagcatattt ttagtgggca
      361 ggactcttgt tctgcctatt tttttatcca aaaagtgcag ttgggaggga gataggctca
      421 tttgggaagg aagtccagtt tttgtttagt gattggggta agatagttgt tatcagatga
      481 gtttatactc ttcgaaaatc aaattcaaac cacgtcaacg tcgccttgcc gtatatatgt
      541 gactgacttc gtcagtccta tctacaacct caaaacagtg ttttgagcag cctgcggcta
      601 gtttcctagt ttgctctttg attttcattg agtattaggg aaaaggagat gaatatgaaa
      661 tttgggaaac gtcactatcg tccgcaagtg gatcagatgg actgcggtgt agcttcatta
      721 gccatggttt ttggctacta tggtagttat tattttttgg ctcacttgcg agaattggct
      781 aagacgacca tggatgggac gacggctttg ggcttggtca aggtggcaga ggagattggt
      841 tttgagacgc gagccattaa ggcggatatg acgctttttg acttgccgga tttgactttt
      901 ccttttgttg cccatgtgct taaggaaggg aaattgctcc actactatgt ggtgactggg
      961 caggataagg atagcattca tattgccgat ccagatcccg gggtgaagtt gactaaactg
     1021 ccacgtgagc gttttgagga agaatggaca ggagtgactc tttttatggc acctagtcca
     1081 gactataagc cttataagga acaaaaaaat ggtctgctct cttttatccc tatattagtg
     1141 aagcagcgtg gcttgattgc caatatcgtt ttggcaacac tcttggtaac cgggattaac
     1201 attgtgggtt cttattatct gcagtctatc attgatacct atgtgccaga tcagatgcgt
     1261 tcgacactag ggattatttc tattgggcta gtcatcgtct acatcctcca gcaaatcttg
     1321 tcttacgctc aggagtatct cttgcttgtt ttggggcaac gcttgtcgat tgacgtgatt
     1381 ttgtcctata tcaagcatgt ttttcacctc cctatgtcct tctttgcgac acgcaggaca
     1441 ggggagatcg tgtctcgttt tacagatgct aacagtatca tcgatgcgct ggcttcgacc
     1501 atcctttcga ttttcctaga tgtgtcaacg gttgtcatta tttcccttgt tctattttca
     1561 caaaatacca atctcttttt catgacttta ttggcgcttc ctatctacac agtgattatc
     1621 tttgccttta tgaagccgtt tgaaaagatg aatcgggata ccatggaagc caatgcggtt
     1681 ctgtcttctt ctatcattga ggacatcaac ggtattgaga ctatcaagtc cttgaccagt
     1741 gaaagtcagc gttaccaaaa aattgacaag gaatttgtgg attatctgaa gaaatccttt
     1801 acctatagtc gagcagagag tcagcaaaag gctctgaaaa aggttgccca tctcttgctt
     1861 aatgtcggca ttctctggat gggggctgtt ctggtcatgg atggcaagat gagtttgggg
     1921 cagttgatta cctataatac cttgctggtt tactttacca atcctttgga aaatatcatc
     1981 aatctgcaaa ccaagcttca gacagcgcag gttgccaata accgtctaaa tgaagtgtat
     2041 ctagtagctt ctgagtttga ggagaagaaa acagttgagg atttgagctt gatgaaggga
     2101 gatatgacct tcaagcaggt tcattacaag tatggctatg gtcgagacgt cttgtcggat
     2161 atcaatttaa ccgttcccca agggtctaag gtggcttttg tggggatttc agggtcaggt
     2221 aagacgactt tggccaagat gatggttaat ttttacgacc caagtcaagg ggagattagt
     2281 ctgggtggtg tcaatctcaa tcagattgat aaaaaagccc tgcgccagta catcaactat
     2341 ctgcctcaac agccctatgt ctttaacgga acgattttgg agaatcttct tttgggagcc
     2401 aaggagggga cgacacagga agatatctta cgggcggtcg aattggcaga gattcgagag
     2461 gatatcgagc gcatgccact gaattatcag acagaattga cttcggatgg ggcagggatt
     2521 tcaggtggtc aacgtcagag aatcgctttg gcgcgtgctc tcttgacaga tgcgccggtc
     2581 ttgattttgg atgaggcgac tagcagtttg gatattttga cagagaagcg gattgtcgat
     2641 aatctcatgg ctttggacaa gaccttgatt ttcattgctc accgcttgac tattgctgag
     2701 cggacagaga aggtggttgt cttggatcag ggcaagattg tcgaagaagg aaagcatgct
     2761 gatttgcttg cacagggagg cttttacgcc catttggtca atagctagaa agaggagagg
     2821 atgaaaccag aatttttaga aagtgcggag ttttataatc gtcgttacca taatttttcc
     2881 agtagtgtga ttgtacccat ggcccttctg ctcgtgtttt tacttggctt tgcaactgtt
     2941 gcagagaagg agatgagttt gtccactaga gctactgtcg aacccagtcg tatccttgca
     3001 aatatccagt caactagcaa caatcgtatt cttgtcaatc atttggaaga aaataagctg
     3061 gttaagaagg gggatctctt ggttcaatac caagaagggg cagagggtgt ccaagcggag
     3121 tcctatgcca gtcagttgga catgctaaag gatcaaaaaa agcaattgga gtatctgcaa
     3181 aagagcctgc aagaagggga gaaccacttt ccagaggagg ataagtttgg ctaccaagcc
     3241 acctttcgcg actacatcag tcaagcaggc agtcttaggg ctagtacatc gcaacaaaat
     3301 gagaccatcg cgtcccagaa tgcagcagct agccaaaccc aagccgaaat cggcaacctc
     3361 atcagtcaaa cagaggctaa aattcgcgat taccagacag ctaagtcagc tatggaaaca
     3421 ggtgcttcct tggccagtca gaatctagcc tactctcttt accagtccta caagtctcag
     3481 ggcgaggaaa atccccaaac taaggttcag gcagttgcac aggttgaagc acagatttct
     3541 cagttagaat ctagtcttgc tacttaccgt gtccagtatg caggttcagg tacccagcaa
     3601 gcctatgcgt cagggttaag cagtcaattg gaatccctta aatcccaaca tttggcaaag
     3661 gttggtcagg aattgaccct tctagcccag aaaatcttgg aggcagagtc aggtaagaag
     3721 gtacagggaa atcttttaga caaggggaaa attacggcga gtgaggatgg ggtgcttcat
     3781 cttaatcctg agaccagtga ttctagcatg gttgcagaag gtgccctact agcccaactt
     3841 tatccatctt tggaaagaga agggaaagcc aaactcacag cctatctaag ttcaaaagat
     3901 gtagcaagaa tcaaggtcgg tgattctgtt cgctatacta cgactcatga tgccgggaat
     3961 caacttttcc tagattctac tattacaagt attgatgcga cagctactaa gactgagaaa
     4021 gggaatttct ttaaaatcga ggcggagact aatctaactt cggagcaggc tgaaaaactt
     4081 aggtacgggg tggaaggccg cttgcagatg attacgggca agaaaagtta tctacgttat
     4141 tatttggatc aatttttgaa caaagagtaa tgttcgtgtt tttagagtta aataattttt
     4201 aaactgtgag aaagattctt cttgcagttt tttctttaca atttttaaaa aacatctgct
     4261 atttattcgg ttaaattctt gtgttttttg gttttttgtg gtaaaatgtg ctcaagtaat
     4321 acgaaaggcg aactttaaaa tgtcaaaaca attgatctat tcgggaaaag ctaaagatat
     4381 ctatacaact gaggatgaaa atcttattat ttcaacttac aaggaccagg cgactgcttt
     4441 caacggtgtc aagaaggagc agattgcagg taagggagtc ttgaataatc agatctcatc
     4501 ttttattttt gagaaattaa atgcggctgg tgtggcgact cactttgtgg agaaactttc
     4561 agacacggaa caactcaata aaaaggttaa gattattcct ttggaagtcg tgctccgcaa
     4621 ctatactgct ggttcctttt caaaacgttt tggtgtggat gagggaatcg ccttggagac
     4681 tccgattgtc gaattttact acaaaaatga tgatttggat gatccattta tcaatgatga
     4741 gcatgtgaaa ttcctacaga ttgcgggtga ccagcagatt gcctacttga aggaagaaac
     4801 gcgtcgtatc aatgaactat tgaaagtctg gtttgctgag attgggctta aattgattga
     4861 ctttaagcta gagttcggtt ttgacaagga tggcaagatt atcttggcag acgaattttc
     4921 accagataac tgccgcttgt gggacgctga tggcaaccac atggataagg atgttttccg
     4981 tagaggattg ggagaactaa ccgacgttta tgagattgtt tgggaaaagt tgcaggaatt
     5041 gaaataatct gtttgcaacg gaaaaccttc gtctctcaac taaaaggact caggctgaaa
     5101 aggtccccca gaccttttca ctctgtagag aactaggtga actaacagat gtttacgaaa
     5161 ttgtctggga aaagttgcag ggtttaaaat aacaacctca aggctgtttg ggaatattgc
     5221 aagagctgaa ataaaggaat aagaattgat ggataaacgt atttttgttg aaaaaaaggc
     5281 tgattttcag gtcaagtcag agagtttggt tagagagctc cagcacaact tgggactgtc
     5341 aagcttgaaa agtattcgta ttgtgcaagt atatgatgta tttgacttgg ctgaggactt
     5401 gtttgcacct gcagagaagc acattttctc tgagcaggga accgaccatg ttttagatga
     5461 agtatctgtg caggcggatc ttgctaacta tgctttcttt gccattgaaa gtctgccagg
     5521 gcagtttgac cagcgtgcag cttcgtcaca ggaagccttg cttttgttgg gaagttcgag
     5581 tgacgtgaca gtcaacacag cccaacttta cttggtgaat aaagatattg atgcgactga
     5641 gttggaagct gtcaaaaact acctgctcaa tccagttgat tctcgtttca aggatatcac
     5701 gacagggatt gccaagcagg agttttcaga gtcagacaag accattccaa aattgacttt
     5761 ctttgaaagc tatacagcag aagactttgc tcgctacaag gccgagcaag ggatggccat
     5821 ggaagtggat gatttgctct ttatccaaga ctactttaag tcaatcgggc gcgtgccaac
     5881 tgagactgaa ctcaaggttt tggacactta ctggtctgac cactgccgtc atacgacttt
     5941 tgagacagag ttgaaacaca tcgacttttc agcttctaaa tttcaaaagc aattgcagtc
     6001 aacctatgac aagtatattg ccatgcgcga ggagttaggt cggtctgaaa aaccacaaac
     6061 cttgatggat atggcgacta ttttcggtcg ttatgagcgt gctaacggac gattggatga
     6121 tatggaagtc tctgacgaaa tcaatgcctg ctcagttgaa attgaagtgg atgttgatgg
     6181 tgtcaaggaa ccttggctcc tcatgtttaa aaatgaaacc cacaaccatc caacagaaat
     6241 tgagccattt ggtggagcgg ctacctgtat cggtggagct attcgtgatc cgttgtcagg
     6301 ccgttcctat gtttaccaag ccatgcgtat ttcaggtgct ggtgatatta cagcaccgat
     6361 ttcggaaact cgtgctggga aattgccaca acaagtcatt tctaaaacag cagctcatgg
     6421 ttattcttca tatggtaacc agattgggct tgccacaacc tacgttcgtg aatacttcca
     6481 cccaggcttt gtagctaaac gtatggaact tggtgccgtt gttggtgcgg ctcccaaggg
     6541 caatgttgtc cgtgaaaaac ctgaagcagg tgatgtgatc atccttctcg gaggcaaaac
     6601 aggtcgtgat ggtgtcggtg gtgcgacggg ctcttctaag gttcaaacag ttgagtctgt
     6661 agagactgct ggtgctgagg ttcaaaaagg aaatgccatc gaagaacgca agattcagcg
     6721 cctcttccgt aatggcaatg tcactcgtct gatcaagaag tccaatgact ttggggcagg
     6781 cggtgtctgt gtggctatcg gtgaattggc agacggtctt gaaatcgacc tcaacaaggt
     6841 gcctcttaaa taccagggct tgaatggtac agaaattgcc atctctgaat cacaagaacg
     6901 gatggcggtc gtggttcgtc ctgaagatgt ggataccttc gttgccgaat gtaacaaaga
     6961 aaatattgat gctgttgtgg tggcgacagt aactgaaaaa ccaaatcttg tcatgcactg
     7021 gaatggtgag acaatcgttg acttggagcg tcgtttcctt gacaccaatg gtgtgcgcgt
     7081 ggttgtcgat gccaaagttg tggacaagga tgtcaaactc ccagaagagc gtcaaacatc
     7141 tgctgaaaca ctggaatcag atacccttac ggttctatct gacctcaacc atgcaagtca
     7201 aaaaggatta cagactgtct ttgactgctc tgttggacgc tcaacggtta atcacccact
     7261 tggtggtcgt taccaactca caccaactga ggcatctgtg cagaaattgc cagttcaaca
     7321 cggtgtgact catactgcgt cggtcattgc tcaaggtttc aacccatatg tagctgaatg
     7381 gtctccatac cacggtgctg cttacgcggt tatcgaagca actgctcgtt tggtggctgc
     7441 tggtgccaac tggtctaagg ctcgtttctc ttaccaagag tatttcgagc gtatggataa
     7501 acaagcagag cgttttggtc agccagtagc cgcccttcta ggctctattg aagctcagat
     7561 tcaacttggc ttgccatcta tcggtggtaa ggactccatg tctggtacct ttgaagaatt
     7621 gaccgttccg ccaaccttgg ttgcctttgg ggtgacgacg gcagatagcc gtaaggtgct
     7681 ctctccagaa tttaaagctg ttggagaaaa tatctactac atcccaggtc aagccctctc
     7741 tgcagagatt gattttgact tgattaagaa aaattttgct cagtttgaag caagccaagc
     7801 tgaccataaa gtgacatctg catcagctgt caaatacggt ggtgtagttg aaagtttggc
     7861 tcttgctacc tttggaaact atattggtgc agaggtgacc ttgcctgaac ttaaaacagc
     7921 tttgacagct caattaggcg gctttgtctt cacatctcct gaagaaattg ctggagtaga
     7981 gaagattgga caaacgaaag cagactttac actgactgtc aacggtgtga agctagatgg
     8041 acacaagctt gacagtgcat ttcaagggac attggaagaa gtttacccaa cagaatttac
     8101 ccaagcgaaa gaactagaag aagtaccagc tgtggcatca gatgttgtga ttaaagccaa
     8161 agaaaaggtt gaaaaacctc tggtttacat cccagtcttt ccaggaacca actcagaata
     8221 tgattcagct aaggccttcg aaaaagaagg tgcagaggtc aatttggtgc cattcgtgac
     8281 cttgaatgaa gaagctattg tcaagtcagt tgaaactatg gttgacaata tcgacaagac
     8341 taatattctc ttctttgccg gtggattctc ggctgcggat gaaccagatg gttcagctaa
     8401 gtttatcgtc aatatcctgc ttaatgaaaa agtgcgtgtg gctattgata gctttatcgc
     8461 ccgtggtggc ttgattatcg gtatttgtaa tggattccaa gccttagtca aatcgggtct
     8521 cctaccatac ggaaactttg aagatgctaa cagtactagt ccaaccctct tctacaatga
     8581 tgccaaccaa cacgtggcca agatggtgga aactcgcatt gccaatacca actcaccatg
     8641 gttggctggt gtgcaagtgg gcgatatcca cgctattcct gtttcgcacg gtgaagggaa
     8701 gtttgtcgtg acggctgagg aatttgcaga gctccgtgac aatggacaaa ttttcagcca
     8761 atacgttgac tttaacggta aaccaagtat ggattctaag tacaatccga atggttctgt
     8821 ccatgccatc gaaggaatta ccagcaagaa tggtcaaatc atcggtaaga tgggccactc
     8881 agaacgttat gaggatggtc ttttccaaaa tatcccaggc aataaagacc aacacctgtt
     8941 cgcatcagcg gttaaacatt tcactggaaa ataagactta cagattttct aatagatagt
     9001 atcagtaatg taaaagtcat gtaaatctag ctcttgatga ttacaaatga aaattaggta
     9061 taaaaaatga catacgaagt aaaatctctt aatgaagaat gtggtgtttt cggtatttgg
     9121 ggacatccag atgctgctaa gttgacctat tttggactcc acagtcttca acaccgtggt
     9181 caggaggggg caggaatcct ctccaatgat caaggacaac tgaagcgcca tcgtgacatg
     9241 gggcttttat cagaagtttt cagaaatcca gccaatttgg ataaattgac aggagctggt
     9301 gcgattgggc atgtgcgtta tgcgactgct ggcgaagctt ctgttgataa catccaaccc
     9361 ttcctcttcc gttttcacga tatgcagttt ggcttggctc ataatggaaa tctgaccaat
     9421 gcagcctctc tcaagaaaga actggaacaa agaggagcaa ttttcagcgc gacttcggac
     9481 tctgaaatct tggctcacct cattcgtcgc agtcataatc ctagcctgat gggcaaaatc
     9541 aaggaagcgc tcagccttgt caaaggtggt tttgcctata tcttgctgtt tgaggacaag
     9601 ttgattgcgg ctcttgaccc taatggcttt cgtccgcttt ctatcgggaa aatggccaac
     9661 ggagcggtgg ttgtttcctc tgaaacctgt gcttttgagg tcattggtgc cgagtggatt
     9721 cgtgatttga agccaggtga gattgtgatc attgatgacg agggcattca gtatgacagc
     9781 tatacagatg atacccagtt ggcgatttgt tctatggagt atatctactt tgcccgccct
     9841 gattctaata tccacggtgt caatgtccat acggcacgta agagaatggg agcgcaattg
     9901 gcgcgagaat ttaagcatga ggcagatatt gtagttggtg tgcccaattc ttccctaagc
     9961 gcggctatgg gatttgcgga agaatcaggc ttaccaaatg aaatgggtct gatcaaaaac
    10021 caatacaccc agcgaacttt tatccaaccg actcaagaat tgcgggagca aggagtgcgg
    10081 atgaaactgt ctgctgtttc gggtgttgtc aaaggcaaac gtgtggtcat ggtggatgat
    10141 tccattgtac gtggaacaac ctctcgtcgt atcgttcagc tcttgaaaga agcgggtgcg
    10201 actgaggttc acgttgccat tggaagtcct gcactagcgt atccatgttt ctacgggatt
    10261 gatatccaga cccgtcagga gctgattgca gccaatcata cggtcgaaga aactcgccaa
    10321 atcattggtg cggacagtat gacttatctt tcaattgatg gcttgattga gtcgattggt
    10381 atcgaaacag atgcaccgaa cggtggtctc tgtgtcgctt actttgacgg tgactaccca
    10441 acgcctctct atgactacga agaagactat cgtagaagtt tggaagaaaa gaccagtttt
    10501 tacaagtagg cgacagattc tccattaaag aaaaggaaaa aacaaatgac aaataaaaat
    10561 gcatatgccc agtcgggtgt ggatgttgaa gcgggttatg aagttgttga acggattaaa
    10621 aagcacgtgg cccgtacgga gcgtgcaggt gtcatgggag ctcttggtgg ctttggtggt
    10681 atgtttgacc tttccaagac tggggttaaa gaacccgtct tgatttcagg gactgacggt
    10741 gtcggaacca agctcatgtt ggctatcaag tacgacaagc acgataccat tgggcaggac
    10801 tgtgtggcca tgtgtgtcaa cgacatcatt gctgcaggtg cggaacccct ctattttctc
    10861 gactacgtag cgacagggaa gaatgaacca gctaagctag aacaagtggt tgctggtgtg
    10921 gcagaaggtt gtgtgcaggc tggtgctgcc ctcatcggtg gggaaacggc tgaaatgccg
    10981 ggcatgtacg gcgaagacga ctatgacttg gctggttttg cggtcggtgt ggctgaaaaa
    11041 tctcaaatca ttgacggttc aaaggtggta gagggagatg ttcttctcgg acttgtttca
    11101 agtgggattc actcaaatgg ttactctttg gttcgtcgtg tctttgcgga ttacacaggt
    11161 gaggaagtcc taccagaatt ggaaggcaag aaacttaagg aagttctact tgagccgact
    11221 cgtatctatg tcaaggctgt cttgccgctc atcaaagaag agttggtcaa cggcattgcc
    11281 cacatcacag gtggtggctt tatcgaaaat gtccctcgta tgtttgcaga tgacctagct
    11341 gctgaaattg atgaaagcaa agttccagtg cttccaattt tcaaagccct tgaaaaatat
    11401 ggtcagatta aacacgaaga aatgtttgaa atcttcaata tgggtgtggg acttatgttg
    11461 gcggtcagcc ctgaaaatgt agagcgtgta aaagaattgt tggatgaagc agtctatgaa
    11521 attggtcgca tcgtcaagaa agaaaacgaa agtgtcatta tcaaatgaaa aaaatagcgg
    11581 tttttgcctc tggtaatggc tcaaattttc aggtgattgc cgaagaattt ccagtggagt
    11641 ttgtcttttc agaccatcgt gatgcctatg tgcttgagcg tgcaaagcag ctcggcgttc
    11701 tgtcctatgc ttttgaactc aaggagtttg agagcaagac agactacgaa gcagcccttg
    11761 tcgaactctt ggaagaacac cagattgact tggtttgcct agcaggctac atgaaaatcg
    11821 ttggaccaac cttattgtcg gcttatgaag gtcggattgt caacattcat ccagcctact
    11881 tgccagaatt tccaggagct catgggattg aggatgcttg gaatgctggc gtgggtcagt
    11941 ctggtgtgac cattcactgg gtggattcgg gtgtggatac aggccaggtc atcaaacagg
    12001 ttcgtgtgcc acgactagct gatgatacca ttgacagatt tgaagctcgc atccatgaag
    12061 cagagtacag gttgtatccg gaagtagtga aggctctatt tacagattga ctttttgatg
    12121 attcatatga tatctttgat tttaaattgg agtcagtgtt tgttgaagac ggcttcaaac
    12181 ggaggtattt gtaatgttag aatctaaaaa aacaactcga tatgtatttt atgtctatct
    12241 gatgttatta acttggggaa tcttatttaa gtttgaaaca aatcctgaat ttatagcatt
    12301 tttcttagct ccaaggtata tcaattggat tccattttca gaaccactaa tagtcgatgg
    12361 aaaaattgtt tttgctgaaa tgttatttaa tctgatttcc tttattccat taggtgtttg
    12421 tttccctttg ataaaaacta atttatctag tttaagaata gtcgggacag gtttcttgat
    12481 tagtttattg tttgagtgct tacagtatat tttagcaata ggtataacag atataacgga
    12541 tttgacttta aatacgctag gtgtctgtgt aggcttactg atttatcaaa tttttataag
    12601 agtgttcaaa tcacagacta gaaaatggat caatatctta ggtatgctta gccttggttt
    12661 tgcttatctt gttttactgt tactgcattt aattggtgtt taactaatga ttaaaaagga
    12721 gaatataatg actaaacgcg tcttaatcag cgtctcagac aaagcgggca ttgttgaatt
    12781 tgcccaagaa ctaaaaaaac ttggttggga gattatctcg acaggtggaa ctaaggttgc
    12841 ccttgataat gctggggtgg agaccattgc gatcgatgat gtgacgggct tcccagagat
    12901 gatggatggc cgtgttaaga ctcttcatcc aaatatccac gggggtcttc tcgctcgtcg
    12961 tgatttggat agccacttgg aagcagccaa tgagaaccaa attgagctta ttgaccttgt
    13021 agtggtcaac ctttatcctt tcaaggaaac gattctcaaa ccagacgtga cgtacgctga
    13081 tgctgtggag aatattgata ttggtgggcc atctatgctt cgttcagcag cgaagaatca
    13141 tgccagcgtg acggttgtgg tagaccctgc tgactataca gtggttttgg acgagttgtc
    13201 agccaatggt gaaacgactt acgaaacgcg tcaacgtttg gcagccaagg ttttccgtca
    13261 cacagcggct tatgacgctt tgattgcaga atacttcaca gctcaagtgg gcgaaagcaa
    13321 acctgaaaag ttgaccttga cctatgacct aaaacaagct atgcgttatg gggaaaatcc
    13381 ccagcaggat gcggacttct accagaaagc tttgccgact gattactcca ttgcatcagc
    13441 caaacagctt aacggtaagg aattgtcatt caacaatatc cgcgatgcag atgctgccat
    13501 tcgtattatc cgtgacttca aagaccgtcc aaccgttgtg gctctcaagc atatgaatcc
    13561 atgtggtatc ggccaggcgg atgacatcga gacggcttgg gattatgctt atgagtctga
    13621 cccagtgtct atctttggtg gtatcgctgt tcttaaccgt gaggtggatg ctgcgacagc
    13681 tgagaagatg cacggcgttt tcctcgaaat catcattgca ccaagctata cggatgaagc
    13741 gctagccatt ttgatcaata aaaagaaaaa cttgcgtatc cttgccttgc catttaatgc
    13801 tcaagaggct agcgaagtgg aagcagaata cacaggtgta gtcggtggac ttctcgtgca
    13861 aaatcaagac gtggtcaagg aaagcccagc tgactggcaa gtggtgacca aacgtcagcc
    13921 aaccgagaca gaagcgactg ctcttgagtt cgcttggaag gctatcaagt acgtcaaatc
    13981 aaatggtatt atcgtgacca acgaccacat gacacttggt gttggtccag gtcaaaccaa
    14041 ccgtgtggct tctgttcgcc ttgccattga ccaagccaaa gatcgtctgg acggggcggt
    14101 ccttgcttca gatgccttct tcccatttgc ggataacgtg gaagaaatcg ccaaagcagg
    14161 aattaaggcc atcatccagc ccggtggctc tgtccgtgac caagaatcca tcgaagcagc
    14221 ggataaatac ggcttgacta tggtctttac aggtgtgaga cattttagac attaagaaga
    14281 taaaagggaa gaaaacagtt tctttccttt tttggcttaa aatgctaact gaaacaagat
    14341 taaaacgaac ttttttgata taatgttggt aaataattcg caaaagaggt tgaggaatga
    14401 aactgcttgt tgtcggttct ggtggtcgtg agcatgcgat tgctaaaaag ttacttgaat
    14461 caaaagacgt ggaaaaagtc tttgtagctc ctgggaatga tgggatgact ctggatggtt
    14521 tggaattggt aaatatctct atttccgaac attctaaatt gattgacttc gcaaagacca
    14581 atgatgttgc ttggaccttt atcggtccag atgatgccct tgctgctggt atcgtggatg
    14641 attttaacca agctggactt aaggcctttg gtccgactag ggctgcagcg gagctggagt
    14701 ggtccaagga tttcgccaag gaaatcatgg tcaaatacgg cgttccgaca gcaacatatg
    14761 gcacattttc agatttcgag gaagccaagg cctatatcga aaagcatggt gcgcctatcg
    14821 tagtcaaggc ggatggcttg gcacttggga agggtgtcgt cgttgctgag acggttgagc
    14881 aagcggtcga agccgctcat gagatgcttt tggacaataa atttggtgac tcaggtgcgc
    14941 gcgtggttat tgaggaattc cttgaaggag aggaattttc actctttgcc tttgtcaatg
    15001 gtgataagtt ctacatcatg ccaacggctc aggaccacaa acgtgcctat gatggcgaca
    15061 aagggcctaa cacgggtggt atgggtgcct atgcgccagt cccacactta ccacagagtg
    15121 tagttgatac agcggttgac accattgtca agccagttct agaaggggtg attaaagaag
    15181 gtcgccctta tctgggagtt ctttacgcag ggcttatcct gacagctgat ggaccgaaag
    15241 tcattgagtt caacgctcgg ttcggagatc cagaaactca gattatcttg cctcgcttga
    15301 cctctgactt tgctcaaaat atcacagata tcctggatag caaggagcca aatatcatgt
    15361 ggacggacaa gggtgtgact ctgggtgtgg ttgtcgcatc caagggctac ccgctagact
    15421 atgaaagggg cgttgagttg ccagcaaaga cagaaggcga tgtcatcacc tactatgcag
    15481 gggctaagtt tgcggaaaat agcagagcac tgctctcaaa cggcggacga gtttatatgc
    15541 tcgttaccac agcagatacc gtcaaagaag cccaagccag catctaccaa gaactatccc
    15601 aacaaaaaat agaaggactc ttctaccgaa cagatatcgg aagcaaggca attaagtaaa
    15661 gatataagaa taacgcgccg tagtcgccaa acacgataat ggtcgtcgtg gtgaaaagac
    15721 cagaacagtg aatgttctgg tcagggggaa acttggagac cttaggctca aagtttagga
    15781 atgaaaccga aggtttgctt ccgcctccat cacctaagac cattatcaaa aagaaaaata
    15841 aaaattcaca aaatacgtta atgatcgtat ggtttgcgag cgttagcgag ctaatataga
    15901 acaatcaccg ccgttgtgaa agaacgattg gatgataatc caatcgttca gggaaattgg
    15961 aagaccttgg gtttccaatt taggcatgag acacctttgg tggctgctgc cgtccctcac
    16021 aagctaaggt gattgttgaa aaagaggaaa aaggagaaga aatgaaacca gtaatttcca
    16081 tcatcatggg ctcaaaatcc gactgggcaa ccatgcaaaa aacagcagaa gtcctagacc
    16141 gcttcggtgt agcctacgaa aagaaagttg tttccgcaca ccgtacacca gacctcatgt
    16201 tcaaacatgc agaagaagcc cgtagtcgtg gcatcaagat catcatcgca ggtgctggtg
    16261 gcgcagcgca tttgccaggc atggtagctg ccaaaacaac ccttccagtc attggtgtgc
    16321 cagtcaagtc tcgtgctctt agtggagtgg attcactcta ttctatcgtt cagatgccgg
    16381 gtggggtgcc tgttgcgacc atggctatcg gtgaagctgg agcgactaac gcagctctct
    16441 ttgccctccg tctcctctct gtagaagata agtccattgc ggatgcactt gccaactttg
    16501 ctgaagaaca aggaaaaatc gcagaggagt cgtcaaatga gctcatctaa aacaatcgga
    16561 attatcggtg gcggtcaact gggtcagatg atggccattt ctgctatcta catgggccac
    16621 aaggttatcg cgctggatcc tgcggcggat tgcccggtct ctcgtgtggc ggaaatcatt
    16681 gtggcacctt ataacgatgt agacgccctc cgtcagttgg cagaccgttg cgatgtcctc
    16741 acttacgagt ttgaaaatgt cgacgctgac ggtttggatg ccgttatcaa ggatggacaa
    16801 ctccctcaag ggacagacct gctccgcatt tcgcaaaatc gtatttttga aaaggacttt
    16861 ttgtcaaaca aggctcaagt caccgttgca ccttacaagg ttgtgacctc aagccaagat
    16921 ttggcagata tcgacttgtc gaaaaactat gttctcaaga ctgcgactgg tggctacgat
    16981 ggtcatggac aaaaggttat tcgttcagaa gcagacttgg aagcagccta tgcgctagca
    17041 gactcagcag actgcgtctt ggaagaattt gtcaactttg accttgagat ttctgtcatc
    17101 gtgtcaggaa atggcaagga ggtgacgttt ttcccagttc aggaaaatat ccaccgcaac
    17161 aatatcctgt ctaagaccat tgtaccagcc cgtatttctg aaagtctagc agacaaggct
    17221 aaagctatgg cagtgcgaat cgcagaacaa ctcaacttgt ctggaactct ctgtgtggaa
    17281 atgtttgcga cagctgatga catcattgtc aatgaaatcg ccccacgacc acataactct
    17341 gggcactatt ctattgaagc ctgtgatttc tctcagtttg acacccatat tctgggtgtt
    17401 ctcggagcac cattaccagt catcaaactc catgcgccag ccgttatgct taatgtcctc
    17461 ggtcagcatg tcgaggctgc tgaaaaatat gtcacagaaa atccaagcgc ccacctccac
    17521 atgtatggta aaatagaagc aaagcataat cgtaagatgg gacatgtgac tttgtttagt
    17581 gatgtgccgg atagtgtgga agagtttggg gaagggattg atttttagga caagtctatg
    17641 atacaaatta tcgttaatac atttattgaa aaggataaga ctggagcagt tgttgaagtg
    17701 ttgtatgcca gtgctgacca agataaggta caagctaaat atgaagaact agctgcacaa
    17761 taccccgaaa attatttagc tatctatgat gtaccgctgg atacggattt gaatacacta
    17821 gatcattacc cgtctgtgtt tattgaaaaa gaggagtttg agtagaaatc ttggtttacc
    17881 tagatagctt attcccaaca gcttaagaag aaaggaaaaa ttaacacatg atcaaccgtt
    17941 actctcgccc tgagatggcg aatatttgga gtgaagaaaa taaataccgt gcttggcttg
    18001 aggtggaaat cctctctgac gaggcatggg ctgagttggg ggaaatccct aaggaagatg
    18061 tggctttgat tcgcaagaag gcggactttg acatcgaccg tattttggaa attgagcagg
    18121 agacgcgcca cgatgtggtg gctttcacgc gtgcggtttc tgagactctt ggtgaagagc
    18181 gcaagtgggt tcactatggg ttaacttcta ccgacgtggt ggatactgct tatggttacc
    18241 tctacaagca ggccaacgac atcatccgtc gtgaccttga aaacttcact aatatcatcg
    18301 ctgacaaggc caaggagcac aagttcacca tcatgatggg gcgtactcat ggtgtgcacg
    18361 ctgagccgac aacttttggt cttaaattgg caacttggta tagcgaaatg aaacgcaata
    18421 tcgagcgttt cgagcatgcg gctgctggtg tagaagctgg taagatttct ggtgcggttg
    18481 ggaactttgc caatatccca ccatttgtag aggagtatgt ctgcaacaag cttggtatcc
    18541 gtgctcaaga aatctctaca caagtccttc ctcgtgacct tcacgctgag tactttgcag
    18601 ttcttgccag cattgcgact tcaatcgaac gtatggcgac tgagattcgt ggtctacaaa
    18661 aatctgagca acgcgaagta gaagagttct ttgctaaagg gcaaaaaggg tcttcagcaa
    18721 tgcctcacaa acgcaaccca atcggttctg aaaatatgac tggtctggcg cgtgtcattc
    18781 gtggtcacat gattacggct tatgaaaacg tcgctctctg gcacgaacgc gatatttccc
    18841 actcatcagc tgagcgtatc atcacaccag atacgaccat tttgattgac tacatgctca
    18901 accgttttgg aaatatcgtc aagaacttga cagtcttccc agaaaatatg atccgaaaca
    18961 tgaactcgac ttttggtctt atctttagcc aacgggctat gttgacattg attgaaaaag
    19021 gcatgacccg tgagcaagcc tatgacttgg tgcaaccaaa aacagcctac tcttgggaca
    19081 accaagtaga ctttaaacca cttcttgagg cagattcaga agtaacatca cgtctcactc
    19141 aagaagaaat cgatgaaatc ttcaacccag tttattacac caaacgagtg gatgatatct
    19201 ttgaacgtct tggactaggt gattaattaa aaaataaaca gcgagcttca atctcgctgt
    19261 ttatttttta tcgaaaagac ttagtcttct tttcttttag tgagtccata ggctgctagt
    19321 gtggacatga gtcctgcgac tactagtcct gcagaatcgt gagttcctgt ttcaggaagt
    19381 tttttctctg ttaccacagg agctggatct tgaggaagag ctttgctttc ctcagcagga
    19441 gcagttgatg gagctggttg gcttgggatt tctagttttg gtttttcttc agcaatagcg
    19501 gcttgtccgt tttcatcgcc tacatgtgtt accatagttc cgacttcgac tatttgagta
    19561 acggcttcct gtgctacgac actatttaca agtgttttca cttccttacc atcggcagaa
    19621 gtgctcacag agtagaagtt gctacgatgt ccattgacgc ccttagtaat gacttgtgtt
    19681 tttcctttga gtaagagtgg attttcacga gtcactgtgg taaatggaat ttcttcttct
    19741 tggatatcca gtctaggttt tacctcagta gttggtgcaa gaccactttc atcacccttg
    19801 tgagttacag gagagccaac ttcaaccact tggtttataa cttctttggt tacctggcta
    19861 tcaaggactg tttctgttgt ttttccattt tcagtgagta cagagatgta atgagttcgt
    19921 tcacctttga ctcctgctgt gataatattt tcctgaccag ctgggaggtt aggattttct
    19981 ttcttgataa cttcaaatgg aatttcttca gttcttgtga tgagttctgg tctggtttca
    20041 acattggcag ccacttcatt ttcatctagg cttcctgaat gagttgcagc tggtttgagg
    20101 ccttgaagag cggcttttag gttggctaca agtgtgtcaa gctcagcttg tttattacgg
    20161 ttgaggttgt aatttagagc tgttttagct gcgtcaaggg cctcaagact ttctttacta
    20221 tatccttcta agtttgtagg aattttagct aattcttcgc ggagagcatt ataattagca
    20281 cggaagtagt ctttgttgtg gtctgcaaag gcagtcatga gttcaaagat ttcctcttcc
    20341 ttgtattcag cgcttggtct atctgcccag attgaaagca tacttccgac tgttggaaga
    20401 tctacttcag gatatttggt agaagctagt tgattgaatg gtgtttttcc agtattctca
    20461 atagctttct tgaggaaacc accaccatct tctggttttt gaccaagaat gtagtatcag
    20521 tcaccgttgg tattcaagaa tttatagcct ttgcttgcta ggtgttgagg tgatgcgagg
    20581 ttatatcccc accagccttt agaccagtaa gaaatcaaga catctttgtc aaactgaaca
    20641 tcgtccttgt cttcatagta gaagccatcg ttgaaggcca ttggttgaag ccctctttct
    20701 ttggccatag ctgcgagggt gttggcatat tcggcaaatt tgccatagag ttgataccac
    20761 ttgaggtagt accagccttg ggcactagtc gcatcgttgg cgtattcgtc agtaccaaag
    20821 ttgaaaatct ttgttttacc tgcaaagaag tccatgtatt taccgatgag ggcttttaca
    20881 aagttcatcg cttcttcgtt tttcaagtcc atagttgttt ttgaaacttt atcaaagtgg
    20941 gcttgaggat ttttaatacc taatttttcc atggcaacca gcatagcatc catgtgacct
    21001 ggactgttaa tagctgggat gagaccgatg tccttagatt tagcgtattc aattagctct
    21061 gttacttctg cctgtgttag tgcagtaccg tttggatcgt cgtagtaagc tttagttcct
    21121 tcgataatag cttttttaac gtcatcacta gcataggttt ttccgttggc agtaatggtc
    21181 atatcatcga gtagaaagcg aagtccgtca tttcctagaa ggagatgaac atcagaatat
    21241 ccgagctcac tggccttgtc tacgatgcgt ttgagctggt ccagagtaaa gtatttgcgt
    21301 ccagcatcga ttgagattac cttgtttttg gcaagttttt caacctcacg tttagcttct
    21361 tcttcttttt gagcttcagg cgtgagggtc aagttgttga cagtttcttg aagtctagca
    21421 atggcttgat caatcgtatc ttgttgggca cggctaaggt tgctatcgag agagcgaata
    21481 gctttttcag cttcttttac ggccgcgacg ctttctgcag tataacggtt caggtctttt
    21541 ggtacctcgt taagtgcttg ctctgcagat tcataatcag ctgcgaagta ttcagcgttg
    21601 gcatttgcaa aatgacgcat gagtttgaag aggcgtgatg gtgaataacg tgcagatgga
    21661 gtgtcagccc aagcagctac cataccaccg atgattggga tatcagctcc ttctgttttt
    21721 ggtacagaag tgattggtgt gtttttaata ccattgagcc cctgatcgag attgtaccag
    21781 ccttggccat cagcgtttcg tccaagaacg tagtaccaag catcattggt attaaggatt
    21841 tggtgacctt tttcagctag tagtttagaa gaagcgacat cgtagcctcc ccaaccacca
    21901 gtccacatag aaacgatgat gtctttgtca aaactaccaa agcttgtgtc gctattgtag
    21961 tagataccgt cgttaaaagc cattggtttg agaccgtgcg attttacaat acgagcgagg
    22021 tcattggcgt aggcaataaa tttttcatag ccttttacag ggtagccttc gtttggatag
    22081 tatttatcag cttgaagcac actccaacct ttagcatctg tcgcatcatt ggcatattca
    22141 tcaagtccga tgttgaagat ttcagtcttt ttcgcgaaat aagcagcata cttgtcgata
    22201 agggcttttg taaaagcgac agcttgttcg ttgtcaagat tgacagtacg ggctgattcc
    22261 ttcccaaaat agctaaagtt agggttttgg attcccaatt ctttcatggc attgagaatc
    22321 gcatccatgt gaccaggact atttactgtc ggaatgagac cgataccttt atctttggca
    22381 tagttaatca gatctgtcat ttgactttct gttaagtgat tgccgtttgg atcgttgtaa
    22441 taatcatttg tacctttttc aatggcgcgt ttgacatcgt cactggcata ggtcttgccg
    22501 ttagctgtga tgctcatatc gtccaacatg aaacggagtc catcatttcc gactaatagg
    22561 tgtaaatcag tgtagccata atgtttcgct ttatcgatga tttccttgag ctgttctggt
    22621 gagaaatatt tacgtccagc atcaatagaa acaattttct ttttcgctag tttttcattt
    22681 acagttgcag cacgttcctt tcctgcctct gtttccggtt tgtcagcctc tgctttcgct
    22741 tcatcttttt cagctggttt atccttgtca gtcttgtctg tatttgactc tttagaatca
    22801 acctctttcg cttctttctt tttaggacta gcttcttctg cctttttatt agcagtttct
    22861 ttttcagcag aagttggagt taccacttct gctttatcac taggagttga actaacttcc
    22921 tcttgtggtt tttcttctgt ttttggaaga ctagctacct tatcagtagc tggagtttct
    22981 gtttctacag tttttagagc ttctggttga agctctgttt taggtgtttc ctcagtccga
    23041 ttttcggatg attgagggga atcagaaacc gtatggatgg tcggttggtt ttctgtagta
    23101 gtaggagtaa ctccatcggc tgcaacagtc tgtgcttgga aggcaaatcc aattagaaca
    23161 gaagctgctc ctacagcgta tttacgaata gaaaaacgct gttgtttttc atgtttcatt
    23221 gcaaaacctc ctgattgcat tgttatattg atagcgacta tataaatcaa cgcctttatt
    23281 ttatttcttg ttttaacgag agtcaagagg agatgacaaa aaactataat aagtataaaa
    23341 aaatataaaa tttaaactta agatttcaga ttggtcgaaa aaatacatat atatatctag
    23401 tataattttt ggttctattt ctataaaata ttccacaaat tatagaatct tccaaaaata
    23461 ggtaagcgct acctttttgg tgtggtataa taagcataga aaaagcccga gcgattaggc
    23521 tcaggctttc ttcttagtga tcacggtcac atgagatgaa tttaatcttg tagtaatcag
    23581 atcgtttgta agtttcactg tattctaaaa cttggccagt tgattcgagt ttggtgattt
    23641 tagtttgtag gacagtaggg aattgttcat cgactccgag gactgaagcc gcatgttctg
    23701 gagttggaaa gactatttcg ttgatttctt caaagtgttc atcattcatg tgaatgtggt
    23761 agtctaactt gaaacgatta tagatagaac tatagtattc aaggtttgga taatttgcgt
    23821 tgatatattg ttctgggatg taggatgtat ggtagatata aacgacaccg tttgattcgc
    23881 ggatacgttc aatcttgtag tagaattgat cgccgcgtag acccaatttt tccaagtaaa
    23941 caagcttgtt tccgcgttca attgaaagaa cagttacctt atcatcttta gcattgaaga
    24001 gttcaatatc tgaaaactct acaagcttgt gtttgcgtgc acgtgaaacg aaggttcctt
    24061 ttccttgttg gcggacaata tagccatctt tggcaaggtc gtttaaggcg cgaacaactg
    24121 tgatagagct gacatcgtac attgaaatga gttctgcttc agtgtaaaat ttatctccac
    24181 tgctaaactg cccagagatg attttatttt ttaattcgtc ttttatgtat tgatatttgg
    24241 gaattgccat atttttacct ctattttcct tctccataga aaattttacc ataaaagcga
    24301 aaaaaatagt agtcttttga ataaaaaatt agaataatag tttgaaaaat taagcgatat
    24361 agggattgct catattttat caatgtattc tatacaaaat tattttatgt cctatattcc
    24421 gaatttaaag agcgtttgaa atgggtctaa aaatgtttga aactaattga atgtgtgtat
    24481 aatagtaaaa atgcagtaaa tatcccgaca atttctattg acaattcaaa cagattggtt
    24541 tataattaat ataacaacaa atgaaagcgc aaactttcgt ggtcggaagg tagttttatg
    24601 acacgatttg agatacgaga tgatttctat ctcgatggaa aatcatttaa gattttatct
    24661 ggtgccattc attattttag ggttcctcca gaggattggt atcattcgct ctataacttg
    24721 aaggctcttg gttttaatac ggtagagact tatgttgctt ggaatttaca cgagcctcgt
    24781 gaaggtgagt ttcattttga aggtgatctg gatttagaga aatttctcca aatagcgcag
    24841 gatttgggtc tctacgcaat tgtgcgtccg tctccattta tctgtgcgga atgggaattc
    24901 ggtggcttac cagcttggct cttgaccaag aacatgcgaa ttcgctcatc cgacccagca
    24961 tatatcgagg cagttggtcg ctactatgat cagttattgc caagactggt gcctcgtttg
    25021 ttggacaatg gtggcaatat tctcatgatg caggttgaaa atgagtatgg ttcttacgga
    25081 gaagataagg cttacctgag agcgattcga cagctaatgg aagagtgtgg cgtaacctgt
    25141 cccctcttta catcagatgg tccatggcga gctactctga aagctggaac cttaattgaa
    25201 gaggacctct ttgtaacagg aaactttggt tctaaggcac cttacaactt ttcgcagatg
    25261 caggaattct ttgatgaaca tggtaagaaa tggccactca tgtgtatgga gttctgggat
    25321 ggttggttca atcgctggaa agaaccgatt atcacacggg atcctaagga attggcagat
    25381 gcagttcgag aggttttgga acaaggctct atcaatcttt acatgttcca cggtggtaca
    25441 aactttggtt tcatgaatgg ttgctcagct cgaggaactt tggacctgcc acaagttaca
    25501 tcttatgatt acgatgccct tctggatgaa gaaggaaatc caactgctaa atatcttgca
    25561 gtcaagaaga tgatggcaac acatttttca gagtatccgc agttggaacc actctacaaa
    25621 gagagtatgg agttggatgc tattccacta gttgaaaaag tttctttgtt tgaaacctta
    25681 gatagcttgt caagtcctgt agaaagtctc tatcctcaaa agatggagga gctgggacaa
    25741 agttatggct acctacttta tcgaacagaa acaaactggg atgcagaaga agaaagactt
    25801 cgtatcattg atggtcgaga tagggcccag ctgtatgtcg atggtcagtg ggttaaaact
    25861 caatatcaga cagagattgg ggaagatatt ttttatcaag gtaaaaagaa agggctatct
    25921 aggttagata tcttgataga aaatatgggg cgtgtcaact atgggcataa gttcttagcg
    25981 gatacgcaac gtaagggaat tcggacaggg gtctgtaagg atctgcattt cttactaaac
    26041 tggaaacact atccactccc actagacaat cctgagaaaa ttgatttttc aaaaggatgg
    26101 actcaaggac aaccagcctt ttacgcttat gactttacag tcgaagagcc aaaagatact
    26161 tacctagact tgtctgagtt tggtaaggga gttgcctttg tcaatgggca gaatctagga
    26221 cgtttttgga acgttggccc aactctctca ctttatatcc ctcatagcta tctcaaggaa
    26281 ggtgccaacc gtatcattat ctttgaaaca gaaggtcaat ataaagaaga gattcattta
    26341 actcgtaaac ctacactaaa acatataaag ggggaaaact tatgacaatt gtaggatgcc
    26401 gtattgatgg acgtttgatc cacggacaag tagccaatct ttgggctgga aaactaaatg
    26461 tttcacgcat tatggttgta gacgacgaag ttgtcaacaa cgatattgaa aagagtggtt
    26521 tgaaacttgc gacaccacca ggtgtgaaat tgagtatttt gccagttgag aaagctgcag
    26581 ccaatattct tgctggtaaa tacgatagcc aacgtctctt tatcgtggct cgtaaaccag
    26641 accgcttcct tggtttggta gaagcaggtg taccacttga aacccttaat gttgggaata
    26701 tgtctcaaac accagaaact cgttctatta cacgttctat caacgtagta gacaaggatg
    26761 tggaagactt ccacaaactg gcagaaaaag gtgttaaact tactgctcag atggttccaa
    26821 atgatccaat ttcagacttt ttgagcttat taaaatagga aaaaaatttt taggaggtca
    26881 ttgttatgat acaatggtgg caaattttac ttctcacttt gtactcagct tatcaaatct
    26941 gtgatgagtt gacgatcgtt tcatctgcag gttcccctgt atttgctggt ttcattactg
    27001 gtttaatcat gggagatgtg actactggtt tacttatcgg tggtaacttg caactgttcg
    27061 ttcttggggt tggtaccttc ggtggtgctt ctcgtatcga cgcaacttct ggtgcggttc
    27121 ttgcgacagc cttctctgtt tcacaaggaa ttgatgcacc gcttgccatt actacaatcg
    27181 ctgtaccagt agcagctctc ttgacttact tcgacgttct tggtcgtatg actactacct
    27241 tcttcgctca ccgtgtggat gctgcaatcg aacgctttga ctataaaggt attgaacgca
    27301 actacttgct tggtgcgatt ccgtgggctc tatctcgtgc ccttccagtc ttctttgccc
    27361 ttgcttttgg tggtgccttt gtacaatcag tagtagactt cgttgaagcc tacaaatggg
    27421 ttgcagatgg cttgacactc gcaggacgta tgcttccagg tcttggattt gcaatcttgc
    27481 ttcgttacct tccagttaaa cgtaaccttc actaccttgc tatgggattt ggtttgacag
    27541 ctatgttgac tgttctttac tcatatgtaa caggtcttgg tggcgctgtt gctggtatcg
    27601 taggtactct tcctgctgaa gttgctgaaa aaattggttt cgtgaacaac ttcaaaggtt
    27661 tgtctatgat tggtatttct atcgtaggta ttttccttgc agtgcttcac ttcaaaaata
    27721 gccaaaaagt agctgtagca gcaccttcta caccatcaga aagtggggaa atcgaagatg
    27781 acgaattcta attacaaact tacaaaagaa gattttaatc aaatcaacaa acgtagcttg
    27841 tttactttcc aattaggttg gaactacgaa cgtatgcaag cttctggtta cctttacatg
    27901 atcttgcctc agttgcgtaa aatgtatggt gatggaactc ctgaattgaa agaaatgatg
    27961 aaagttcata ctcaattctt caatacttca ccattcttcc ataccattat cgctggtttt
    28021 gaccttgcca tggaagaaaa agatggtgta ggttcaaaag acgctgttaa cggtatcaag
    28081 acaggtttga tgggaccatt cgctcctctt ggggatacaa tctttggttc acttgtacct
    28141 gctatcatgg ggtcagtcgc agcaactatg gctatcgctg gccaaccttg ggggatcttc
    28201 ctttggattg cagttgcagt agcgtatgac atcttccgtt ggaaacagtt ggaatttgct
    28261 tacaaagaag gggttaacct tatcaacaac atgcaaagta ccttgacagc tttgattgac
    28321 gctgcatctg tacttggtgt cttcatgatg ggtgctcttg tagcaacagt gattaacttt
    28381 gaaatttctt acaagttgcc aatcggtgaa aagatgattg atttccaaga catcttgaac
    28441 caaatcttcc cacgtttgct tccagcaatc tttactgcct ttatcttctg gttgcttggt
    28501 aaaaaaggta tgaactctac taaagctatc ggtattatta tcgtacttgc tttggctctt
    28561 tctgcccttg gtcactttgc acttggaatg taattcctta tgactaaatc attaattttg
    28621 gtgagccatg gtcgcttctg tgaggagctt agaggtagca cagaaatgat tatgggccca
    28681 caagacaaca tttacacagt agctcttctt ccagaagatg gcccagaaga atttactgct
    28741 aaatttgaag ctgttattga aggattggat gatttcctag tctttgcgga tcttctcggt
    28801 gggacacctt gtaatgtggt gagtcgcttg atcatggaag gtcgtgatat tgacctttac
    28861 gcagggatga atcttccaat ggtgattgaa tttatcaatg cgagccttac aggcgcagat
    28921 gcggactaca agagccgtgc tgcagaaagc attgtgaaag ttaatgacct gttagcgggc
    28981 ttcgatgatg acgaagatga ataatactct tcgaaaatct cttcaaacta cgtcaacgtc
    29041 gccttgccgt aggtatatgt tactgacttc gtcagtctta tccggcaacc tcaaagcagt
    29101 gctttgagca gcctgcggct agtttcctac agattttagt tggaactcga ttcaattcat
    29161 gtgacaacgt gaaaatcgtt agagcatttt atatagaata tacatgggaa tggagcttac
    29221 tcccattccc atatttaata gaaaaagagg aactcaatgc tacattatac aaaagaagac
    29281 ttgctcgaat tgggtgcaga aatcactacg cgtgaaatct accaacagcc tgatgtatgg
    29341 agagaagctt ttgaatttta tcaagcaaaa cgtgaagaaa ttgcagcctt tctacaagaa
    29401 atcgctgata aacatgacta tattaaggtt atcttgacag gtgctgggac ttctgcttat
    29461 gtgggagata ccttgctacc ttactttaag gaagtctatg acgaacgcaa atggaatttc
    29521 aatgctattg cgacaacaga tatcgttgcc aaaccagcaa cctatttgaa aaaagatgtg
    29581 gcaactgtcc ttgtgtcttt tgctcgtagt gggaactcgc ctgaaagtgt ggcgactgtt
    29641 gatttggcca aatccttggt ggatgagctt tatcaagtga cgattacttg tgcagcagat
    29701 ggtaaattgg ctcttcaagc tcacggtgat gaccgtaatc tcttgctctt gcaaccagct
    29761 gtctctaatg atgctggatt tgccatgact tctagcttta cgtctatgat gttgacagct
    29821 ctcttggtct ttgatcctac agaatttgct gttaagtctg aacgttttga agttgtatct
    29881 agtcttgccc gtaaagtttt agacaaggta gaagatgtca aagagctcgt tgatttagac
    29941 tttaaccgtg tcatctatct aggcgctggt cctttctttg gacttgctca tgaagctcag
    30001 ctcaagattt tggaattaac tgctggtcaa gttgcgacca tgtatgaaag cccagttggc
    30061 ttccgtcacg gtccaaaatc tcttatcaac gacaatacag ttgttttggt ctttggtaca
    30121 acgacagact acactcgtaa gtacgacttg gacttggttc gtgaagttgc tggtgaccag
    30181 attgctcgtc gtgttgtgct tttgagtgat caagcttttg gtcttgaaaa tgtcaaagaa
    30241 gtggtccttg gttgtggcgg tgttttgaat gatatttacc gtgtcttccc ttacatcgtt
    30301 tatgcccaac tctttgctct attgacttca ctcaaggtag aaaataaacc agatacaccg
    30361 tctcctacag gtacagtaaa ccgtgtagta caaggtgtca taattcacga atatcaaaag
    30421 taagacagtg tttatgaatt cttgacaaga ggatttgtaa attatcagat aaaccataga
    30481 ttgtcagtac gctttctatg gtttgtttgc ttgagagaaa tagtaaaagg agaacagaat
    30541 gaaagcatac acagagcgtg tatttggaaa tgttgagggc gaggatgtct tggcctatcg
    30601 atttgagaca gacggtggct accaacttga ggttatgact tatggtgcga ccatcttgcg
    30661 ctatgtcgca cctgacaagg ctggaaattt tgccaatgtt atcttgggat ttgatgactt
    30721 tgatagttat gtaggcaata gtcccaagca tggagcaagt gtaggtcctg tagcgggtcg
    30781 tattgcaggt gcgacctttg agctcaatgg taagacctat gaccttgagg ttaataatgc
    30841 tagcaactgt aatcacagtg gttcaactgg ttgggattcc agcttgtttg aagttgaaga
    30901 agtaagcgat catggcttga ctctctacac agagcgtaca gatgggacag gagggttccc
    30961 tggaaatctc aagatttgga tcagttatca cttggaagaa actggtgcct atgaaatcag
    31021 ctacaaggta acgaccgatc aggatacgct ggtcaatcca accaaccaca gctatttcaa
    31081 cttgtctggt gatttcacgc agacgattga ccgtcatgtc ttccaactaa acacagaggg
    31141 catttactca atcgctcctg acggtgttcc tgccaaaact ccagaagcca accgtgatgt
    31201 ggtcaaacac gtctacaatg gtaccttgtt gaaggatatc tttgcagaag aagatgagca
    31261 aatccagctg gcatcaggtt tggatcatcc atttgccctt cctgcaggcc atgacaatgc
    31321 tggattcctt tatgaccaaa attcaggtcg cttcctgctt ttcaagacag aagctccttg
    31381 ctttgtggtc tacacagcaa actttgtgga tgaaagtgtc atcataggag gtcagccaat
    31441 gctacagcac aatgggattg ctcttgaagc gcaagcttta ccagatgcca ttcacagtga
    31501 ccttaaaggc caagtcattc ttaaagctgg tcaaaccttc accagtaaga cacgttatga
    31561 acttgttgtg aaataaaaaa gctggtcaaa aagtctttaa aattaaaata acgcatattg
    31621 tcaggtgatg aaaagtttga taatatgcgt tttattatga aacgatttac tttatattat
    31681 cctgaaatgg tgttattttt gagttatctg gcttgacttt cttgatggaa attatataat
    31741 agttgtaata ataataatta caacgagaaa agaggaccat ttatgaccta tgaatacaag
    31801 agtcacattt atttggcaga gacagcttta aatgtaaagg atttggccag tcaaacagcc
    31861 ttttatcagc aagttattgg tttagaaatc ttatctcaaa ctgagacaga gtccattcta
    31921 ggccttggtg gaaaagtctt ggtacagctg attcaagcac aagagagcgg agaagtgagg
    31981 gaacattatg gtctttacca tttggccatt ctcttgccca cacgcaaggc tttggctgat
    32041 gtcttgaaac acctgacaga tttacaaatt cctcttgtcg gcggtgcaga ccatggttac
    32101 agtgaagcta tttacttgga agatttggag ggaaatggca ttgaactcta tcgagataag
    32161 ccagtttcca catgggatat tcgagaagat ggacgcatta tcggagtgac ggaggctctt
    32221 gcggcgcagg acatttatga gttgggggaa agagtagagc cctttatcct ggcagaaggt
    32281 acgagaatgg ggcatattca tctttccgtc aaggatagtc gaaagtccag ccagttttat
    32341 caaaaggtgt tagggctaga ggataaattc agtgtgccta gcgctagttg gattgcggct
    32401 ggagattacc atcatcattt agcagttaac gaatggggag gaaaaggtct ggcttcgcgt
    32461 aagcaaggct taccaggttt agcctactat gtcatcgaag tcgcacataa agaagaactg
    32521 ttaacgattg cccaacgagc acaagaagtt gacgcaccaa tcaaatggat gacatcgagc
    32581 caattggaaa tcacagactc agatggcatc gtgacccgta ttcgtttagc agggtaaaat
    32641 taggaatttt taataagtta taagtattgt tagtttgctt tttgaacggt ctttggtaac
    32701 agaactgcgt gagctggatg ggcgagaatt ttgacaaaga agctgattag agatataatg
    32761 aagaaaaatc gagcaaggaa aaagaaatga caaattcaaa gtatattact tgtttgaaac
    32821 gttcagaggg ccagttgtgt gggattcaaa agatgattga aggagatcgt gactgtgctg
    32881 atattgtgac gcaactgaca gcagtgagat ctagtgtaga gcgcgtgatt gagatgataa
    32941 ttaccgaaaa tcttactgaa tgtatcaatc agccactaga tgattctgaa gctcaaaagg
    33001 aacgcctaga aaaggctatc cgatacttga ttaaacggaa ataaatgaat gttttgagac
    33061 gatatgtgca aaggtctatt aactattaga atagtttaat gtgagtcaaa agaagttgta
    33121 aaaaatcttt aaaattaaaa aaacgcatag tatcaggtgt tgaatgtatt gaccccaaaa
    33181 agttagattt tttctgtcta acttttgggg tgcagttcat aagaaccttg gtaatatgcg
    33241 ttttattatg tagagactta tactcttcga aaatctcttc aaaccacgtc agcttcacct
    33301 tgccgtagat atggttactg acttcgtcag ttctatccac aacctcaaaa cagtgttttg
    33361 agctgacttc gtcagttcca tccacaacct taaaacagtg ttttgagctg acttcgtcag
    33421 ttccatccac aaccttaaaa cagtgttttg agcagcctgc ggctaacttc ctagtttgca
    33481 ctttaatttt cattgagtat tacttcatct tctcatgaaa ttgagttttt gcccagctcc
    33541 tcttgattat tctacttgac caggccaagc attcatacca ccttctacgt tggtaacggt
    33601 gaggccttgg gagatgagaa attggcaggt agatgcagag cggactcctc cttgacaaat
    33661 aacatagtat tcatggtcag gtttgagttc tttgtagcct tgctctaagg tacttaatgg
    33721 aagatttttg gcacctggtg catgtcctgc ttggaattca tgtgcttcac ggacatcgat
    33781 aagttctaga ttttcatttt ggtattttcc ataaaagtca gccatgctga tactagtttc
    33841 catattctac tccttctggg ttaaccattt tgtatagtga ataagcgccg tcaaggtttt
    33901 gggcggtaaa tcctgcttgc ttgaggatag gctctgcgat atagctgcgc aaaccactgt
    33961 ggcagctgac gatgtaggct tgttttttgt ccagttcatc agcttcctcc ttgacacttt
    34021 gtcagttttt gatacaatag tacaaaatta gaggaggtgg gctatgattc agaaacatgc
    34081 gattcctatt ttagagtttg atgacaatcc tcaggcggtt atcatgccca atcacgaggg
    34141 gctggacttg cagttgccaa agaagtgtgt ttatgcattt ttagatgagg agattgaccg
    34201 ctatgcaagg gaagtagggg cggattgtgt cggcgaattc gtttctgcta ccaagaccta
    34261 tccagtctat gtcatcaact acaagggtga ggaggtctgt ctggctcagg ctcctgttgg
    34321 ctccgctcca gcagcccagt ttatggattg gttgattggc tatggtgtgg agcagattat
    34381 ctctactggg acctgtggtg ttctagctga tatagaggaa aatgcctttc tagtccctgt
    34441 tcgcgctctg cgagatgaag gagccagtta ccactatgtg gcaccttctc gttatatgga
    34501 aatgcagcca gaggctattg ctgctattga ggaagttttg gaagacagag ggattcctta
    34561 tgaagaagtc atgacctgga cgacagacgg tttttaccga gaaacggctg aaaaggtggc
    34621 ttatcgtaag gaagaaggct gtgctgttgt ggagatggag tgttctgctc ttgcggcagt
    34681 agctcaattg cgtggggttc tctggggtga attgttgttc acagcagatt ctctagcgga
    34741 cttggaccag tacgacagtc gtgactgggg ctcggaagct tttaataagg cgctagaact
    34801 gagtttagca agtgttcacc acctttagtt gtactggcaa aggatttgtt ttatcataaa
    34861 atgtctagct catacttttc aaaaatatgt ttaaacgagg tcaccttcct cttgtcctaa
    34921 gcatgttgag gttgggaaaa atctttaaaa tcagaaaaac gtatcatatc aggtgatgaa
    34981 aaccttgata ctatgcgttt tatgttggta agatttagag tgagatgaaa tgatactctt
    35041 cgaaaatctt ttcaaaccag gtcagcttca ccttgccgta ggtatatgtt actgacttcg
    35101 tcagtcttat ccggcaacct caaaacggtg ttttgagctg acttcgtcag ttctatttgc
    35161 aacctcaaaa cagtgttttg agctgacttc gtcagttcta tttgcaacct caaaacagtg
    35221 ttttgagcaa tctgtgacta gctttctaat cgatgccttg gttctcattg cctataatca
    35281 aaaagagaaa ttttctcctg aaaagcatat agagtagctg gcgttaaaag ctcctgtctt
    35341 gcttttttga cctatagtca catctatcaa gtattgttct tgcctaagct atcaataaaa
    35401 aggtggcatt ttttaggctt ggtgttagta gattttgcct tatcctatct aagtcatttc
    35461 gaacttttta tggtacaatg gaaacatgtt attcaaatta tctaaggaaa aaatagagct
    35521 aggcttatct cgtttatcgc cagcccgtcg tatttttttg agttttgcct tggtcatttt
    35581 actaggctct cttcttttga gcttgccctt tgtccaagtt gaaagctcac gagcgactta
    35641 ttttgatcat cttttcactg ctgtctctgc agtctgtgtg acaggtctct caacccttcc
    35701 agtagctcac acctataata tatggggcca aataatctgt ttgctcttga ttcagatcgg
    35761 tggtctaggg ctcatgacct ttattggggt tttctatatc cagagcaagc aaaagcttag
    35821 tcttcgtagc cgtgcaacta ttcaggatag ttttagttat ggagaaactc gatctttgag
    35881 aaagtttgtc tattctattt ttctcacgac ctttttggtt gagagcttgg gagctatttt
    35941 gcttagtttt cgccttattc ctcaacttgg ctggggacgt ggtcttttta gttccatttt
    36001 tctagcgatc tcagccttct gtaatgccgg ttttgataat ttagggagca ccagtttatt
    36061 tgcttttcag accgatttac tggtcaatct ggtgattgca ggcttgatta ttacaggcgg
    36121 ccttggtttt atggtctggt ttgatttggc tggtcatgta ggaagaaaga aaaaaggacg
    36181 tctgcacttt catacgaagc ttgtactatt gttgactata ggtttgttgt tatttggaac
    36241 ggcaactact ctctttcttg agtggaacaa tgctggaacg attggcaatc tccctgttgc
    36301 cgataaggtt ttagttagct tttttcaaac agtgacgatg cgaacagctg gcttttctac
    36361 gatagattat actcaggctc atcctgtgac tcttttgatt tatatcttac agatgtttct
    36421 aggtggggca cctggaggaa cagctggggg actcaagatt acgacatttt ttgtcctctt
    36481 ggtctttgca cgaagtgagc ttctaggctt gcctcatgcc aatgttgcga gacgaacgat
    36541 tgcgccgcga acggttcaaa aatcctttag tgtctttatt atctttttga tgagcttctt
    36601 gataggattg attctgctag ggataacagc caaaggcaat cctcccttta tccacctcat
    36661 atttgaaacc atttcagctc ttagtacagt tggtgtaacg gcaaatctga ctcctgacct
    36721 tgggaaattg gctctcagtg ttatcatgcc acttatgttt atgggacgaa ttggtccctt
    36781 gaccttgttt gttagcttgg cagattacca tccagaaaag aaagatatga ttcactatat
    36841 gaaagcagat attagtattg gttaagaaag gaaagagcat gtcagatcgt acgattggaa
    36901 ttttgggctt gggaattttt gggagcagtg tcctagctgc cctagccaag caggatatga
    36961 atattatcgc tattgatgac cacgcagagc gcatcaatca gtttgagcca gttttggcgc
    37021 gtggagtgat tggtgacatc acagatgaag aattattgag atcagcaggg attgatacct
    37081 gcgataccgt tgtagtcgcg acaggtgaaa atctggagtc gagtgtgctt gcggttatgc
    37141 actgtaagag tttgggggta ccgactgtta ttgctaaggt caaaagtcag accgctaaga
    37201 aagtgctaga aaagattgga gctgactcgg ttatctcgcc agagtatgaa atggggcagt
    37261 ctctagcaca gaccattctt ttccataata gtgttgatgt ctttcagttg gataaaaatg
    37321 tgtctatcgt ggagatgaaa attcctcagt cttgggcagg tcaaagtctg agtaaattag
    37381 acctccgtgg caaatacaat ctgaatattt tgggtttccg agagcaggaa aattccccat
    37441 tggatgttga atttggacca gatgacctct tgaaagcaga tacctatatt ttggcagtca
    37501 tcaacaacca gtatttggat accctagtag cattgaattc gtaaagaggg atgacccctc
    37561 ttttttgatg cctaagatgg caaatagaga cagaagcccc ttgtcttcta gtaaaagttc
    37621 ttcaaaggct ggactttatg gtaaaataga aagaagtgac aagagagagt aatactcaat
    37681 gaaaatcaaa gatcaaacta ggaaactagc tacgggctgc tcaaaacact gttttgaggt
    37741 tgtggataga actgacgaag tcagctcaaa acactgtttt gaggttgcag atagaactga
    37801 cgaagtcagt aacatctata cggcaaggcg acgttgacgc ggtttgaaga gattttcgaa
    37861 gagtataaga aaaaatcagt cccctaaagg agtagattat gaagttattg tctatcgcaa
    37921 tttctagcta taatgcagca gcctatcttc attactgtgt ggagtcgcta gtgattggtg
    37981 gtgagcaagt tgggattttg attatcaatg acgggtctca ggatcagact caggaaatcg
    38041 ctgagtgttt agctagcaag tatcctaata tcgttagagc catctatcag gaaaataaat
    38101 gccatggcgg tgcggtcaat cgtggcttgg tagaggcttc tgggcgctat tttaaagtag
    38161 ttgacagtga tgactgggtg gatcctcgtg cctacttgaa aattcttgaa accttgcagg
    38221 aacttgagag caaaggtcaa gaggtggatg tctttgtgac caattttgtc tatgaaaagg
    38281 aagggcagtc tcgtaagaag agtatgagtt acgattcagt cttgcctgtt cggcagattt
    38341 ttggctggga ccaggtcgga aatttctcca aaggccagta taccatgatg cactcgctga
    38401 tttatcggac agatttgttg cgtgctagcc agttctaact gcctgaacat actttttatg
    38461 tcgataatct ctttgtcttt acgccccttc agcaggtcaa gaccatgtac tatctgcctg
    38521 tcgatttcta tcgttatttg attgggcgtg aggaccagtc tgtcaatgag caagtgatga
    38581 ttaagtgcat tgaccagcaa ctcaaggtca atcgactctt gatagaccaa cttgatttgt
    38641 cccaagtgag tcatcccaaa atgcgagaat atctgctgaa tcatattgaa ctcacgacgg
    38701 tgatttccag taccctgctc aaccgatctg gaacagcgga gcatctggca aaaaaacgcc
    38761 aattgtggac ctatattcag cagaaaaatc cagaagtctt tcaggctatt cgtaagacca
    38821 tgttgagccg tttgaccaaa cattctgtct tgccagatcg caaactgtcc aatgtcgtct
    38881 atcaaatcac caaatctgtt tatggattta attaatataa gtgttttata agagggattt
    38941 aagaaaaatt ttaacttttt cttagtcctt tttaatttca ggagattata ctagagtcat
    39001 caaataaaga aagactctaa ggagaatcct atgaaattca atccaaatca aagatatact
    39061 cgttggtcta ttcgccgtct cagtgtcggt gttgcctcag ttgttgtggc tagtggcttc
    39121 tttgtcctag ttggtcagcc aagttctgta cgtgccgatg ggctcaatcc aaccccaggt
    39181 caagtcttac ctgaagagac atcgggaacg aaagagggtg acttatcaga aaaaccagga
    39241 gacaccgttc tcactcaagc gaaacctgag ggcgttactg gaaatacgaa ttcacttccg
    39301 acacctacag aaagaactga agtgagcgag gaaacaagcc cttctagtct ggatacactt
    39361 tttgaaaaag atgaagaagc tcaaaaaaat ccagagctaa cagatgtctt aaaagaaact
    39421 gtagatacag ctgatgtgga tgggacacaa gcaagtccag cagaaactac tcctgaacaa
    39481 gtaaaaggtg gagtgaaaga aaatacaaaa gacagcatcg atgttcctgc tgcttatctt
    39541 gaaaaagctg aagggaaagg tcctttcact gccggtgtaa accaagtaat tccttatgaa
    39601 ctattcgctg gtgatggtat gttaactcgt ctattactaa aagcttcgga taatgctcct
    39661 tggtctgaca atggtactgc taaaaatcct gctttacctc ctcttgaagg attaacaaaa
    39721 gggaaatact tctatgaagt agacttaaat ggcaatactg ttggtaaaca aggtcaagct
    39781 ttaattgatc aacttcgcgc taatggtact caaacttata aagctactgt taaagtttac
    39841 ggaaataaag acggtaaagc tgacttgact aatctagttg ctactaaaaa tgtagacatc
    39901 aacatcaatg gattagtt
//
LOCUS       DAVBGY010000013        38077 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVBGY010000013 DAVBGY010000000
VERSION     DAVBGY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38077
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.013"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..96
                     /locus_tag="VQ620_000707"
     CDS             <1..96
                     /locus_tag="VQ620_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2763377.1"
                     /translation="IIDYIDYYNNKRIKIKLKGLSPVQYRTKSFG"
     gene            complement(141..782)
                     /gene="plsY"
                     /locus_tag="VQ620_000708"
     CDS             complement(141..782)
                     /gene="plsY"
                     /locus_tag="VQ620_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET2763378.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            918..2861
                     /gene="parE"
                     /locus_tag="VQ620_000709"
     CDS             918..2861
                     /gene="parE"
                     /locus_tag="VQ620_000709"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HET2763379.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            3281..5752
                     /gene="parC"
                     /locus_tag="VQ620_000710"
     CDS             3281..5752
                     /gene="parC"
                     /locus_tag="VQ620_000710"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET2763380.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            5884..6906
                     /locus_tag="VQ620_000711"
     CDS             5884..6906
                     /locus_tag="VQ620_000711"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HET2763381.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            6962..7666
                     /locus_tag="VQ620_000712"
     CDS             6962..7666
                     /locus_tag="VQ620_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2763382.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGFPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            7731..8420
                     /locus_tag="VQ620_000713"
     CDS             7731..8420
                     /locus_tag="VQ620_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HET2763383.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            8417..9340
                     /locus_tag="VQ620_000714"
     CDS             8417..9340
                     /locus_tag="VQ620_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HET2763384.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            9355..9999
                     /gene="pcp"
                     /locus_tag="VQ620_000715"
     CDS             9355..9999
                     /gene="pcp"
                     /locus_tag="VQ620_000715"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET2763385.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            10167..10397
                     /locus_tag="VQ620_000716"
     CDS             10167..10397
                     /locus_tag="VQ620_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HET2763386.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            10447..10527
                     /locus_tag="VQ620_000717"
     tRNA            10447..10527
                     /locus_tag="VQ620_000717"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10481..10483,aa:Tyr,seq:gta)
     gene            10537..10608
                     /locus_tag="VQ620_000718"
     tRNA            10537..10608
                     /locus_tag="VQ620_000718"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10569..10571,aa:Gln,seq:ttg)
     gene            10770..11972
                     /gene="rpsA"
                     /locus_tag="VQ620_000719"
     CDS             10770..11972
                     /gene="rpsA"
                     /locus_tag="VQ620_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET2763387.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            12058..12555
                     /locus_tag="VQ620_000720"
     CDS             12058..12555
                     /locus_tag="VQ620_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET2763388.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            12555..14210
                     /gene="dnaX"
                     /locus_tag="VQ620_000721"
     CDS             12555..14210
                     /gene="dnaX"
                     /locus_tag="VQ620_000721"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET2763389.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            14238..14432
                     /locus_tag="VQ620_000722"
     CDS             14238..14432
                     /locus_tag="VQ620_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HET2763390.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            14582..15352
                     /gene="sufC"
                     /locus_tag="VQ620_000723"
     CDS             14582..15352
                     /gene="sufC"
                     /locus_tag="VQ620_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET2763391.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITRLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            15380..16642
                     /gene="sufD"
                     /locus_tag="VQ620_000724"
     CDS             15380..16642
                     /gene="sufD"
                     /locus_tag="VQ620_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET2763392.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKINYLERLESRGEGSAKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSYADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            16653..17879
                     /locus_tag="VQ620_000725"
     CDS             16653..17879
                     /locus_tag="VQ620_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2763393.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPASARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            17866..18306
                     /locus_tag="VQ620_000726"
     CDS             17866..18306
                     /locus_tag="VQ620_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET2763394.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            18356..19768
                     /gene="sufB"
                     /locus_tag="VQ620_000727"
     CDS             18356..19768
                     /gene="sufB"
                     /locus_tag="VQ620_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET2763395.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(19999..21240)
                     /locus_tag="VQ620_000728"
     CDS             complement(19999..21240)
                     /locus_tag="VQ620_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET2763396.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITAYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFIDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            21343..22452
                     /locus_tag="VQ620_000729"
     CDS             21343..22452
                     /locus_tag="VQ620_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET2763397.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            22986..23333
                     /gene="ssrA"
                     /locus_tag="VQ620_000730"
     tmRNA           22986..23333
                     /gene="ssrA"
                     /locus_tag="VQ620_000730"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(23445..23532)
                     /locus_tag="VQ620_000731"
     tRNA            complement(23445..23532)
                     /locus_tag="VQ620_000731"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23496..23498),aa:Ser,seq:gga)
     gene            23694..24434
                     /locus_tag="VQ620_000732"
     CDS             23694..24434
                     /locus_tag="VQ620_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2763398.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            24431..25342
                     /gene="pfkB"
                     /locus_tag="VQ620_000733"
     CDS             24431..25342
                     /gene="pfkB"
                     /locus_tag="VQ620_000733"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HET2763399.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGAFLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDVVEAFKWGVACG
                     TATTFSNDLATAEFIKETYGKVEVEKR"
     gene            25339..27291
                     /locus_tag="VQ620_000734"
     CDS             25339..27291
                     /locus_tag="VQ620_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2763400.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSAGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLAISGDAEVYRAANGAK
                     VATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALSVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            27455..29680
                     /locus_tag="VQ620_000735"
     CDS             27455..29680
                     /locus_tag="VQ620_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET2763401.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(29724..30152)
                     /locus_tag="VQ620_000736"
     CDS             complement(29724..30152)
                     /locus_tag="VQ620_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763402.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSNVKE
                     EKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENKSNQ"
     gene            30290..31432
                     /locus_tag="VQ620_000737"
     CDS             30290..31432
                     /locus_tag="VQ620_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2763403.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            31441..32655
                     /gene="thiI"
                     /locus_tag="VQ620_000738"
     CDS             31441..32655
                     /gene="thiI"
                     /locus_tag="VQ620_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HET2763404.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            32792..33616
                     /locus_tag="VQ620_000739"
     CDS             32792..33616
                     /locus_tag="VQ620_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2763405.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTDKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFYSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     KSPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSFANWLHQEAFNRYIECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSKNLPDCLRFFAEKW"
     gene            33638..34377
                     /locus_tag="VQ620_000740"
                     /pseudo
     CDS             33638..34377
                     /locus_tag="VQ620_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            34390..35556
                     /locus_tag="VQ620_000741"
     CDS             34390..35556
                     /locus_tag="VQ620_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET2763406.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            36064..37557
                     /locus_tag="VQ620_000742"
     CDS             36064..37557
                     /locus_tag="VQ620_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HET2763407.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            37570..>38077
                     /locus_tag="VQ620_000743"
     CDS             37570..>38077
                     /locus_tag="VQ620_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2763408.1"
                     /translation="MKKVKLGEILSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLRDHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITRRK
                     FQLDELNLL"
BASE COUNT        11499 a         6916 c         8462 g        11200 t
ORIGIN      
        1 attatagact atattgatta ctacaacaat aaacgaatta agataaaact aaaaggactt
       61 agccctgtgc aatacagaac taaatccttc ggataaatta attgtctaac ttttggggtg
      121 cagttcacag aactggcatt ttatttttta ggatcttgat gggttaggtt caatccccaa
      181 gggaccaaat tttcagtttt atttttgata cgagctatat tgtccttatg acgaatgata
      241 atcaaactag caagtgctaa gataatagcg atgaagagag agtcatagtt actcaggata
      301 aaaccaaaaa gtggaaagag cagaaccccg ataacagccg cgatcgatgc tgtgacacta
      361 gacagtgaaa tcatactgcc aagatagaga gctccaaaga agataatcgc aaggtagaga
      421 cagaagatag gcgcaaatcc gaaaattact ccagcactgg ttgcgacagc cttaccacct
      481 ttaaatcctg caaagatagg gaaggtatgg ccgataacag ccaaaagtcc aaagatgaga
      541 ggagaaacgc cttgtagatg aaaaataatc ggaagcagcg ttgctagggt tcctttgaaa
      601 aagtcaatca caaaggttgc cataccagct ttcttaccta aaatgcggaa ggtattggtc
      661 gttccagtgt taccagaacc atgctcgcgt agattgattt gaaagaatac ttgtccaatc
      721 cagagaccag atggaatcga acccagcaga taggctagga ttaataaaac tattgtaatc
      781 atactcctat tatatcatga attgggaaag aaaagcagag aatctctgct aaaattgtca
      841 catcggagaa agaaaaattt tgcaaaatcc ttggaaaacc tgtagaatag taaagatgaa
      901 cgaataggag gttccttgtg tcaaaaaagg aaatcaatat taacaattat aatgatgatg
      961 ctattcaggt gctagaaggg ttggatgcgg tccgaaaacg tccagggatg tatattggat
     1021 cgaccgatgg cgctggtctt catcacctag tttgggaaat cgttgataat gcagtcgatg
     1081 aagccttgtc tgggtttggt gatcgtatcg atgtaaccat caataaagac ggtagtctaa
     1141 cggttcaaga ccatggacgt gggatgccga caggtatgca cgctatggga attccaactg
     1201 ttgaggttat ctttaccatt cttcatgccg gagggaaatt cggtcaaggt ggctataaga
     1261 catcaggtgg acttcacgga gtgggttctt ccgttgttaa cgccctttct agctggttag
     1321 aagttgaaat tacccgtgat ggcgcagttt acaagcaacg tttcgaaaat ggtggaaaac
     1381 ctgtcacgac tttgaagaaa atcggtacag cacccaagtc taaaacaggc accaaagtta
     1441 cttttatgcc tgacgcgact atattttcta cgacagattt caagtacaat accatttcag
     1501 agcgccttaa tgaatcagcc tttctcttga aaaatgtgac cttgtcttta acggacaagc
     1561 gaacagatga agcgattgag ttccactatg agaatggagt acaagatttt gtttcttatc
     1621 tcaacgaaga taaggaaatc ttgacgccag ttctttactt tgaaggggaa gacaatggtt
     1681 ttcaagtgga agtagccctc cagtacaatg acggattctc agataacatt ctatcctttg
     1741 tcaataacgt tcgcaccaag gacggtggaa cgcacgagac aggactcaag tctgccatta
     1801 ccaaggtcat gaatgactat gcacgtaaaa caggtcttct caaggaaaaa gataaaaacc
     1861 ttgaaggttc agactatcgt gagggactag cggccgttct ttctatctta gttcctgaag
     1921 aacacttgca gtttgaagga cagaccaagg ataaactagg aagcccccta gctcgcccag
     1981 ttgtggatgg aatagtggct gataagttga cctttttcct tatggaaaat ggggaattag
     2041 cttctaacct catccgcaag gctatcaagg cccgtgatgc tcgtgaagca gcacgtaagg
     2101 cgcgtgatga gagccgaaat gggaagaaaa acaagaaaga taagggcttg ttgtctggga
     2161 aattgacccc agcccaatct aagaatcctg ctaagaatga actctatcta gttgaggggg
     2221 actctgccgg tggttctgcc aaacaaggtc gtgaccgcaa gttccaggct attctacctc
     2281 ttcgtggtaa ggttatcaat acagccaagg ccaagatggc ggatatcctc aaaaatgaag
     2341 agatcaatac catgatttat accattggtg cgggtgttgg agcagacttc tctattgaag
     2401 atgccaacta tgataagatc attatcatga ccgatgcgga taccgacggt gcccatatcc
     2461 agaccttgct cttgacattt ttctaccgtt acatgcgtcc gctagtcgag gcaggtcatg
     2521 tctatattgc cctcccacct ctttacaaga tgtccaaagg taaaggaaag aaagaagaag
     2581 tggcctacgc ttggacggac ggagaactag aagaactccg taaacagttc ggtaaaggcg
     2641 ctaccctcca acgatacaaa ggacttggtg agatgaatgc ggaccagctc tgggaaacaa
     2701 ccatgaaccc agaaacacgt accctcatcc gtgtcacaat tgaagattta gcgcgcgccg
     2761 aacgccgcgt caatgttctc atgggagata aggtagaacc acgccgtaaa tggattgaag
     2821 ataatgtcaa gtttacgcta gaagaagcga cagtgtttta atgaaagaaa ataatatgtt
     2881 agtaaactgg aaattagagt cggatgtgaa tgactatgtg aaaaagcagt ttgaaaatct
     2941 tggtttgaaa aaattgcagg attattaatg aagaatctgc tatgagtgct tatctcaaag
     3001 aggcgcttaa gggagcggct aagacacaaa ctaagactaa ttttggaaaa ccagattttc
     3061 atattgaaaa atacaaacag gatagtttgg ttacttatcg taactgaaca cgggaggaat
     3121 tcccgtaaaa tatcttttac tgcataaaaa cctactctac attctttgaa aggagttgaa
     3181 cacgccctag atactgtgtg aaaaagataa attctcttgt gagtttgctt acttcaagaa
     3241 ttttctattt tcacttggta ttttatgggc tttgtatctt atgtctaaca ttcaaaacat
     3301 gtccctggag gacatcatgg gagagcgctt tggtcgctac tccaagtaca ttattcaaga
     3361 ccgggctttg ccagatattc gtgatgggtt gaagccggtt cagcgccgta ttctttattc
     3421 tatgaataag gatagcaata cttttgacaa gagctaccgt aagtcggcca agtcagtcgg
     3481 gaacatcatg gggaatttcc acccacacgg ggattcttct atctatgatg ccatggttcg
     3541 tatgtcacag aactggaaaa atcgtgagat tctagttgaa atgcacggta ataacggttc
     3601 tatggacgga gatcctcctg cggctatgcg ttatactgag gcacgtttgt ctgaaattgc
     3661 aggttacctt cttcaggata tcgagaaaaa tacagttcct tttgcatgga actttgacga
     3721 tacggagaaa gaaccaacgg tcttgccagc agcctttcca aacctcttgg tcaatggttc
     3781 gactgggatt tcggctggtt atgccacaga cattcctccc cataatttag ctgaggtcat
     3841 agatgctgca gtttacatga ttgaccaccc aactgcaaag attgataaac tcatggaatt
     3901 cttgcctgga ccagacttcc ctacaggggc tattattcag ggtcgtgatg aaatcaagaa
     3961 agcttatgag actgggaaag ggcgcgtggt tgttcgttcc aagactgaaa ttgaaaagct
     4021 aaaaggtggt aaggaacaaa tcgttattac tgagattcct tatgaaatca ataaggccaa
     4081 tctagtcaag aaaatcgatg atgttcgtgt taataacaag gtagctggga ttgctgaggt
     4141 tcgtgatgag tctgaccgtg atggtcttcg tatcgctatc gaacttaaga aagacgctaa
     4201 tactgagctt gttctcaact acttatttaa gtacaccgac ctacaaatca actacaactt
     4261 taatatggtg gcgattgaca atttcacacc tcgtcaggtt gggattgttc caatcctgtc
     4321 tagctacatc gctcaccgtc gagaagtgat tttggcgcgt tcacgctttg acaaagaaaa
     4381 ggctgagaaa cgtctccata tcgtcgaagg tttgattcgt gtgatttcga ttttggatga
     4441 agtcattgct cttatccgtg cttctgagaa taaggcggac gccaaggaaa acctcaaagt
     4501 tagctatgat tttacggaag aacaggctga ggctatcgta actttgcaac tgtaccgttt
     4561 gaccaatacc gatgtggttg tcttgcagga agaagaagca gagcttcgtg agaagattgc
     4621 tatgctggcg gctattatcg gtgatgaaag gactatgtac aatctcatga agaaagaact
     4681 tcgtgaggtc aagaagaaat ttgcaactcc tcgtttgagt tctttagaag acactgcgaa
     4741 agcaattgag attgatacag ctagtcttat cgctgaggaa gatacctacg tcagcgtgac
     4801 caaggcaggt tacatcaagc gtaccagtcc acgttccttt gcggcttcca ccttggaaga
     4861 aattggcaag cgtgatgatg accgtttgat ttttgttcaa tctgccaaga caacccagca
     4921 cctcttgatg ttcacaagtc ttggaaatgt catctacaga ccaatccatg agttggcaga
     4981 tattcgttgg aaggacatcg gagagcatct gagccaaacc atcacaaact ttgaaacgaa
     5041 tgaagaaatc ctttatgtgg aagtactgga tcagtttgac gatgcgacaa cctactttgc
     5101 agtgactcgc cttggtcaaa tcaaacgggt agagcgaaaa gaattcactc catggcggac
     5161 ctatagatct aagtctgtca agtatgctaa gctcaaagac gatacagatc agattgtagc
     5221 agtggctccg attaaactag atgatgttgt cttggttagt caaaatggtt atgccctgcg
     5281 tttcaatatc gaagaggttc cggttgtcgg tgctaaggca gcaggtgtca aggctatgaa
     5341 tttgaaagaa gatgatgtcc tccaatctgg ctttatctgt aatacttcgt ccttctacct
     5401 cttgacccag cgtggaagct tgaaacgtgt ttctattgag gaaattctag caaccagccg
     5461 tgccaaacga ggattacaag tcttgcgtga gttgaaaaac aaaccgcatc gtgtcttctt
     5521 ggcaggagca gttgcagagc aaggatttgt tggcgatttc ttcagtacgg aagtggatgt
     5581 gaacgaccaa actctgcttg tccaatccaa taaaggaaca atctatgaaa gccgattgca
     5641 agacttgaac ttgtcagaac gcactagcaa tggaagcttc atttctgaca cgatttcaga
     5701 tgaagaagtt tttgacgctt atcttcagga agtagttact gaagataaat aagtgaaatg
     5761 aagaatcagt tctaagagct ggtttttatt ggagaaattt tctgaaaatt acaaaatata
     5821 cttgctattt tagaaaaata gtgtagaata taagaaatag gtttttagaa taaggagtgg
     5881 aatatgacag taacgattga ttgggaaaac ctcggttttt cctatatgaa attaccttat
     5941 cgctatcttg ctcatttcaa aaatggacaa tgggatcaag gagagcttac agaggatgca
     6001 actttgcata tttcagagtc ttctccaagt cttcactatg gacaacaagc atttgaaggt
     6061 ttgaaagctt atcgtactaa ggatggcagt gttcaactgt tccgtcctga tgaaaatgct
     6121 aaacgtctgc aacgtacatg tgaccgtctc ttgatgccac aagttccgac agacatgttt
     6181 gtagaagctt gtaaagcagt tgtccgtgcg aatgaagaat acgtaccacc atacggaaca
     6241 ggtggaacct tatatcttcg ccctcttttg attggtgtcg gagatattat cggggtaaaa
     6301 ccggcagaag agtacatttt caccatcttt gctatgccag ttggaaatta ctttaaaggt
     6361 ggtttggtcc caaccaactt cttgattcag gatgagtacg accgtgcagc accaaatggt
     6421 acaggtgcgg ctaaggttgg tggaaactat gctgcaagtc tcttaccagg aaaaatggcc
     6481 aagtcacgtc atttctcaga tgttatctat ctggacccat caactcatac aaagattgaa
     6541 gaagtcggat cagctaactt ctttggaatt acagctgata atgaatttgt aacaccattg
     6601 agtccatcta tcttgccatc tattaccaag tattccttgc tttatttggc agaacatcgc
     6661 ttgggattaa ctcctattga gggtgatgtt ccaattgata atcttgaccg ttttgtagag
     6721 gcaggtgcct gtggtacagc agcggttatt tctccaattg gaggtattca acatggtgat
     6781 gatttccatg tattctatag tgaaacagaa gtaggtcctg tgacgcgtaa attatataat
     6841 gaattgacgg gtattcagtt tggcgatatt gaagcgccag aaggttggat tgtaaaagta
     6901 gattaaaata aaccaaagga gattttttat gaaatagaaa aagtggctct taacagcagg
     6961 agtggtcctg agcacgtcag ctattttagt ggcttgtgga aaaactgata aagaagcaga
     7021 tgcaccgaca acattttctt atgtctatgc agtagatcca gcatcattgg gctacagtat
     7081 agcgactcga acatcgagga cagacgttat tggaaatgtt attgatggtt tgatggaaaa
     7141 tgataaatac ggcaatgttg ctccttctca aaaagactat gatttgaaca gtacaggatg
     7201 ggctccaagc tatcaagatc cagcgtctta cttgaatatt atggatccaa aatctggttc
     7261 tgccatgaaa caccttggca ttacgaaagg aaaagataag gatgttgtag ctaaacctgg
     7321 tttggataaa tataagaaat tgttagaaga tgctgtttct gagaccactg acctagagaa
     7381 gagatatgaa aaatatgcca aagctcaagc ttggtcgaca gatagttcat tattgatgcc
     7441 aacagcttca tctggtggtt ttccagttgt aagtaacgta gtaccattct caaaaccata
     7501 ctcacaagtt ggtattaagg gggaaccata tatctttaaa ggaatgaaat tgcaaaaaga
     7561 tattgttaca acaaaagaat ataacgaggt ttttaaaaaa tggcaaaaag aaaaattgga
     7621 atccaatagc aaataccaaa aagaactaga aaaatacatt aaataaggaa tggtattgat
     7681 cttgataaaa tttttaaaat actgtcattt tgaatataaa ggagtttgat atggagtgga
     7741 ttagattaat aggaatagca atcattgttg tgggttttat tttaaaattt gatacaattg
     7801 caacagtagt cttagctggt ttggttacag ctttagtttc aggtgtttct ctcgttgaat
     7861 ttttggagat tttgggaaaa gaatttagca atcagcgagt gctcacgatt tttatggtta
     7921 ccttgcctct tgtggggctg tcagaaacct ttggactcaa gcaacgatca atcgatttga
     7981 ttcgaaagat taaaggtctg acagttggaa acttctatac agtttatttc tttattcgag
     8041 agttagctgg tttcttttca attcgtctag gaggacaccc tcagtttgtc agacctttgg
     8101 ttcaacctat gggagaagca gctgcagagt ctcaattagg tagaaagtta acagaggttg
     8161 aagatgagac aataaaagcg cgtgcggctg cgaatgaaaa ttttggaaat ttctttgctc
     8221 aaaatacgtt tgtaggtgct gggggagtcc tcttgatagg gggaacatta gatcagttag
     8281 gctatgaaag caattatgca gggattgctt ctacatctat tattgttgct gttataacac
     8341 ttattgtagt ggggatttac aattatttat ttgataaaaa attgatatca aaaaagacta
     8401 ggggaggaga acaaaaatga cagagttggc aaagcaacta ttagagttga cctatattgt
     8461 gattggttgt caatttctcc atacagccta ttgtagttat aaagataaaa caaacccagt
     8521 tcgacttggg acatctgcat tttggactct attgtctatt acgtttatag gtggttccta
     8581 tatgccaaat atgagtattg gtattattgt aatcctatta tcgctgttaa cattgtttaa
     8641 gcaagtccgt atcggaacct tgccatcctt agatgaaatg aaagccaata ttgaatctaa
     8701 caggttgaaa aataaaattt ttattccagt tatgctgatg gcaatacttg cgttggtctt
     8761 agcgcaaatg attccagaat ttagcaagat ttcgattagc cttgccgcct tgtttgctac
     8821 aatttctgtt cttgtgatta ccaatagtca ccctaagagt ctgttatcag aaaataatcg
     8881 aatgactcag caagtttcaa caagtgggat tgttcctcaa ttattagggg ctttgggggc
     8941 tatttttact gtagcaggtg ttggtgatgt tatctctcat ctgattagcg gtattgttcc
     9001 ttcagatagt cgctttatag gagttttggc ttatgttctt ggaatggttc tattcacaat
     9061 gattatggga aatgcttttg cagcattcac cgttattaca gcaggtgttg gagttccctt
     9121 tgtatttgct ctgggagcta atccaattgt ggctggtgct cttgccatga cagcaggtta
     9181 ttgtgggacc ttattgaccc caatggctgc taattttaac gctctaccag cagcattgat
     9241 ggatatgaaa gatcagaatg gcgttataaa ggctcaagca ggtgttgctc tagtaatgat
     9301 tgttattcac atattcttaa tgtactttct cgcattttag taaaggaaaa taaaatgaaa
     9361 atattggtta caggttttaa tccttttgga ggtgaaaaga ttaatccagc tttggaggct
     9421 gtaaaattat taccatctga gattaatggg gctgaagttc gctgggtaga aattccaacg
     9481 gttttttata agtcgtcaga agttttagag gcagaaatat tacgatatca accagatgct
     9541 gtactttgta ttggacaagc aggcggcagg accggcttaa cacctgaacg agtggctatt
     9601 aatcaagatg atgctcgcat acctgataac gaaggcaatc aaccaattga tacaccgatt
     9661 cgtattgatg gagcatcggc ctattttagt agtttaccta tcaaagcgat ggtacaagct
     9721 atcaaaaaac agggacttcc ggcagttgta tccaatagtg caggaacctt tgtttgcaat
     9781 catttgatgt accaagctct ttatttagtg gataaaaaat tccctaatat gagagcaggt
     9841 ttcatgcata ttccatatat gatggaacaa gtagtaaata agccgaatac tgcaggaatg
     9901 agtctatgtg atattgtaag aggcatagag gtagctattg aagctattgt agactacaaa
     9961 gataaggatt tgcagttagt aggcggtgag actcactgat aacctaaaaa ggatagtcaa
    10021 ttatgcttgt ttactaacta ttaactatgc taaatcaatt gaggttgttt acataaaact
    10081 ctatatcaga gaagcctgat atagagtttt ttcttgctag ttttaggatt ttcttgtaaa
    10141 atagaaaaag tgaagagagg tatgaaatga gcaagaaaga taaaaaaatc gaaattcaag
    10201 tagcggatgc caaagttaat gttggtaaag acagttttga aggttataca ttgactatcg
    10261 gtaaaaaagt tatcggagaa attgccgaat tagacggaca atttgccatt ataaagaatg
    10321 ggaatgtcga tagtttttat aaaaaattgg aaaaagctgt ggaaattttg attgaaaatt
    10381 ataatttagc aaaataagtc ttgttttttt gaaattttca tgatataata gtccatgttg
    10441 attgtaggag agatagcgaa gaggctaaac gcggcggact gtaaatccgc tccttcgggt
    10501 tcgggggttc gaatccctct ctctccattt cattaatggg gtatagccaa gcggtaaggc
    10561 aagggacttt gactccctca tgcgttggtt cgaatccagc taccccagtt cttaggtaat
    10621 aatcaagata aaaagcaaaa tatcttaggg tattttattt ttataattga aagacgtgaa
    10681 tgatatgaac atgtccttgc gggtgcttag gaaaaaaatt ataagtatgt caagtttaag
    10741 aaaaacttga ttgttggagg attttttaga tgaacgaatt tgaagatttg ctaaatagcg
    10801 ttagtcaagt tgagactggt gatgttgtta gtgctgaagt attgacagtt gatgcgactc
    10861 aagctaacgt tgcaatctct ggaactggtg ttgaaggtgt cttgactctt cgcgaattga
    10921 caaacgatcg tgatgcagat atcaatgact ttgttaaagt aggagaagta ttggatgttc
    10981 ttgtacttcg tcaagtagtt ggtaaagata ctgatacagt tacatacctt gtatctaaaa
    11041 aacgccttga agctcgcaaa gcatgggaca aacttgttgg tcgcgaagaa gaagttgtta
    11101 ctgttaaagg aacgcgtgcc gttaaaggtg gactttcagt agaatttgaa ggtgttcgtg
    11161 gatttatccc agcttcaatg ttggatactc gtttcgtacg taacgctgag cgttttgtag
    11221 gtcaagaatt tgatactaaa atcaaagaag ttaacgctaa agaaaaccgc ttcatccttt
    11281 cacgtcgtga agttgttgaa gcagctactg cagcagctcg cgctgaagta ttcggtaaat
    11341 tggctgttgg tgatgttgta actggtaaag ttgctcgtat cacaagcttc ggcgctttca
    11401 tcgaccttgg tggtgttgac ggattggttc acttgactga attgtcacat gaacgtaacg
    11461 tatcaccaaa atcagttgta actgttggtg aagaaattga agtgaaaatc cttgatctta
    11521 acgaagaaga aggacgtgta tcactttcac ttaaagcaac agtaccagga ccatgggatg
    11581 gcgttgagca aaaattggct aaaggtgatg tagtagaagg aacagttaaa cgtttgactg
    11641 acttcggtgc atttgttgaa gtattgccag gtatcgatgg acttgttcac gtatcacaaa
    11701 tttcacacaa acggattgaa aatccaaaag aagctcttaa agttggtcaa gaagttcaag
    11761 ttaaagttct tgaagttaac gcagatgcag aacgcgtgtc actttctatt aaagctcttg
    11821 aagaacgtcc agcccaagaa gaaggacaaa aagaagaaaa acgtgctgct cgtccacgtc
    11881 gtccaagacg tcaagaaaag cgtgatttcg aacttccaga aacacaaaca ggattttcaa
    11941 tggctgattt gtttggtgat atcgaacttt aatcaaattg aaaattcaca aaatcctttg
    12001 tttactaaac aagggatttt tcttttgtct ctgctccttt tttgatataa tagttctatg
    12061 ttaaaatcag aaaaacaatc acgttatcaa atgttaaatg aagaattgtc cttcctattg
    12121 gaaggcgaaa ccaatgtttt ggctaatctt tccaacgcca gtgctcttat aaaatcacgt
    12181 tttcctaata ccgtatttgc aggcttttat ttgttcgatg gaaaggaatt ggttttaggt
    12241 cccttccaag gaggtgtttc ctgcatccgt attgcactag gcaagggtgt ttgtggtgag
    12301 gcagctcact ttcaggaaac tgttattgtt ggagatgtga cgacctatct caactatatt
    12361 tcttgtgata gtctagctaa aagtgaaatt gtggtgccga tgatgaagaa tggtcagtta
    12421 cttggagttc tggatctgga ttcttcagag attgaggatt acgatgctat ggatcgagat
    12481 tatttggaac aatttgtcgc tattttgctt gaaaagacaa catgggactt tacgatgttt
    12541 gaggaaaaat cttaatgtat caagcacttt atcgaaaata tagaagtcaa aacttctccc
    12601 agttagttgg tcaagaagtt gtggctaaga ctcttaaaca agcggtggag caagagaaaa
    12661 taagtcacgc ttatcttttt tctggtcctc gtggaacggg aaaaaccagt gttgctaaaa
    12721 tctttgccaa ggctatgaac tgtcccaatc aagtgggtgg cgaaccttgc aataactgct
    12781 atatttgtca agcagtgacg gacggtagtt tagaagatgt cattgaaatg gatgcagctt
    12841 ctaataatgg ggtagatgaa attcgcgaaa ttcgtgataa atctacctat gcgcctagcc
    12901 ttgctcgtta taaggtttat atcatagatg aggttcacat gctgtctaca ggggctttta
    12961 atgccctcct aaagacgctg gaagaaccaa cacagaatgt agtctttatt ttggccacta
    13021 ctgaattaca caagattcct gctactattc tatcccgtgt gcaacgtttt gagtttaaat
    13081 caattaagac acaggatatt aaggaacata ttcactatat cttagaaaaa gaaaatatca
    13141 gttctgaacc agaggctgtg gaaatcattg ccagacgggc ggaaggtgga atgcgggacg
    13201 ccttgtctat tttggatcaa gccctgagtt tgacacaggg aaatgagctg acgactgcta
    13261 tctctgaaga aattactggc actattagcc tatcagcctt ggatgattat gtggcggcct
    13321 tgtctcaaca ggatgttccc aaagctttgt cttgcttgaa tcttcttttt gacaatggta
    13381 agagcatgac tcgttttgtg accgatcttt tgcactattt aagagacttg ttaattgttc
    13441 aaacaggggg agaaaatact catcatagtt cagtctttgt agaaaatttg gcacttcctc
    13501 aaaaaaatct gtttgaaatg attcgcttag caacagtgag tttagcagat attaagtcta
    13561 gtttgcagcc caagatttat gctgaaatga tgaccgtccg tttggcggaa atcaagcccg
    13621 aaccagctct atcaggagcg gttgaaaatg aaattgctac gctgagacag gaagttgccc
    13681 gtctcaaaca agagctttct aatgtaggtg cggttcctaa acaagttgca ccagctccta
    13741 gtcgaccagc tacgggcaaa acagtctatc gtgtcgatcg caataaagtg caatctatct
    13801 tacaagaggc cgtcgaaaat cctgatttag cacgtcaaaa tctaattcgt ttgcagaatg
    13861 cctggggaga ggtaattgaa agtctaggtg ggccggacaa ggctctgcta gttggttctc
    13921 aaccggttgc tgccaatgaa caccatgcta ttcttgcttt tgagtctaac ttcaatgctg
    13981 gtcaaactat gaaacgagac aatctcaata ccatgtttgg caatatcctc agtcaggcgg
    14041 caggtttttc acctgagatt ttagctattt ccatggagga atggaaagaa gttcgcgcag
    14101 ccttttcagc caaagccaaa tcttctcaaa ctgaaaaaga agtagaagaa agcctgattc
    14161 cagaaggatt tgaatttttg gctgataaag tgaaggtaga ggaagactaa agaaagattt
    14221 cgtgatacaa taagtttatg aataaacaac aatttattat tatggcgctg tttacagctg
    14281 ctgagaccta ttttttcaat gaagcctgga tgactggccg ctatattatg gcagcctttt
    14341 gggcaatttt actctttaga aatttccgag tcagttatgt gatgggcaaa atcgttgatg
    14401 tcatcgatca gcattttaat aggaaagact agccctcagc ttccagacaa aatcaaagcc
    14461 ttttaggctt ttttttgtta tactagaaaa gtatatttat agaatttttg ctctatttct
    14521 ggggaaatca gacgtttttc tagtaagtac tgtaaaagtt ttgaaaaaga aaggaactat
    14581 catgtcagta ttagagatca aagatcttca cgttgagatt gaaggaaaag aaattttaaa
    14641 aggggttaac ctgaccctga aaacaggaga aattgccgct atcatgggac caaatggtac
    14701 aggtaaatcg actctttctg ccgctatcat gggaaatcca aactatgaag taactaaagg
    14761 tgaagttttg tttgatggcg taaacatcct tgagttggaa gtggatgagc gtgcgcgtat
    14821 gggacttttc cttgctatgc aatacccatc agaaatccct ggaattacca atgctgagtt
    14881 tcttcgtgcc gctatgaatg cgggtaaaga agatgatgag aagatttcag ttcgtgagtt
    14941 tattactagg ctagatgaaa aaatggaatt gctcaacatg aaagaagaaa tggcagagcg
    15001 ttacctcaac gaaggcttct ctggtggtga gaaaaaacgc aatgaaattc ttcaactttt
    15061 gatgttggag ccaacatttg ctcttttgga cgagattgac tcaggtcttg atattgacgc
    15121 tcttaaagtt gtgtctaaag gtgtcaatgc catgcgtggt gaaggttttg gtgctatgat
    15181 catcactcac taccaacgtc ttttgaacta tatcacacct gatgtggtac acgtgatgat
    15241 ggaaggtcgt gttgtccttt ctggtggtcc agaattggct gcgcgtttgg aacgtgaagg
    15301 atacgcaaaa ttagctgaag aacttggcta cgactacaag gaagaattgt aatttcctcg
    15361 tatcttttag gagaagtaaa tgactagaga aaatattaaa cttttttcag aaatgcacgc
    15421 tgaaccaagc tggttggctg atctccgtca aaaagctttt gacaagattg agactttgga
    15481 attaccagtt attgagcgtg tcaaattcca ccgttggaat ctgggtgatg gaacgattac
    15541 agaaaatgag ccatcagcaa atgttccaga tttcacagct ttagatcatc acttgaagtt
    15601 ggtgcaagta ggaactcaaa ctgttttcga acaaactcca gttgagttag ctgaacaggg
    15661 tgttgtcttc acagactttc actcagcttt agaagaaatt ccagagctga tcgaagaatt
    15721 cttcatgtca tctgttaagt atgatgatga caagttggcg gcttaccaca cagcttactt
    15781 taacagtggt gctgtactct atattccaga taacgtagaa atcacagagc caattgaagg
    15841 aattttctac caagatagcg atagcaatgt gccgtttaac aagcatatta tgattatcgc
    15901 tggtaaaaat tctaagatta attatctgga gcgtttagag tcacgcggtg aaggaagtgc
    15961 caaagcaact gctaatatca cagtagaagt gattgcacgt tcaggtgcgc aagtcaagtt
    16021 tgctgctatc gaccgtctag gtgaaaacgt cactgcctac attagccgtc gtggtaaatt
    16081 aggcaacgat gcaagtattg actgggctat cggtgtcatg aacgaaggaa atgtcgttgc
    16141 tgattttgat agtgacttga ttggtaatgg tagctatgct gacctcaagg ttgtagctct
    16201 ttcaagtggt cgtcaggtac aagggattga tactcgtgta actaactatg gctgcaactc
    16261 tatcggaaat atcctacaac atggggttat ccttgaaaaa gcaactttga ctttcaatgg
    16321 tatcggccac atcatcaagg gtgctaaggg agcagatgcc caacaagaga gccgtgttct
    16381 catgctttca gaccaagcgc gttcagatgc taacccaatt cttttgattg atgaaaatga
    16441 tgtcactgca ggacacgcag cgtctatcgg acaagttgat ccagaagaca tgtattacct
    16501 catgagtcgt ggattggata aggcaactgc agagcgtttg gttgttcgtg gtttccttgg
    16561 atctgttatc gtggagattc cagtcaagga agttcgtgat gaaatgattg caactatcga
    16621 agagaaattg tcaaaacgct aaggggaagc ctatgttaga tgtagaagcg attcgcaagg
    16681 attttccaat tttagatcag attgtcaatg atgaacctct ggtctatctg gacaatgctg
    16741 cgacgacaca aaaaccacta gtagttctga aagctattaa cagttactat gagcaggaca
    16801 atgccaatgt tcaccgtggt gtccatacct tagcggaacg agcgacagct tcttatgaag
    16861 ctgctcgtga aaccattcgt aagtttatta atgcaggctc tacaaaggaa gttctcttta
    16921 ccagaggaac gacaaccagc cttaactggg tggcacgctt tgctgaggaa attctcactg
    16981 agggagacca ggtcttgatt tcagtaatgg aacaccattc taatatcatt ccatggcagg
    17041 aagcttgtcg aaagactgga gcagagcttg tctatgtcta tcttaaagac ggtgccttgg
    17101 atatggagga tttgcgagct aaattgactg ataaggttaa atttgtttcc ctagctcatg
    17161 cctccaatgt tcttggtgtg gtcaatccga tcaaggaaat cactcaatta gcccaccaag
    17221 ttggggcaat catggtagtg gatggtgctc aatctacacc tcatatgaag attgatgtcc
    17281 aggacttgga tctggacttt ttcgcctttt cgggtcacaa gatggctggt ccgactggta
    17341 tcggtgtcct ttacggcaaa gaaaagtatc ttgagcaaat gtctccagta gaatttggcg
    17401 gcgagatgat tgattttgtc tacgagcaat ccgctagttg gaaggaattg ccttggaaat
    17461 ttgaggctgg aacgccaaat atggcaggag ctattggact tgcgactgca gttgattatc
    17521 tggaaaagat tggtatggat gccgttgaag ctcatgaaca ggaattgatt gcgtacgtct
    17581 atccaaaact gcaggcaatt gagggattga ccatttacgg ttctcaggat ttggctcaac
    17641 gttcgggtgt tattgccttt aacctaggtg atctccatcc tcacgatctt gcgacggctc
    17701 tggattatga aggagtggct gttcgtgctg gtcaccattg tgcgcaaccc ttgcttcagt
    17761 atttggaagt cccagcatca gctcgtgcaa gtttttatat ctataatacc aaggcagatt
    17821 gcgacaaact agtcgatgcc ctacaaaaga caaaggagtt tttcaatggc actttctaaa
    17881 ctagatagcc tttatatggc agtggtagca gaccattcga aaaatccaca tcaccaaggg
    17941 aagttagaag acgctgagca aatcagtctc aacaatccga cttgtgggga tgtcatcaac
    18001 ctctctgtca agtttgatgc agaggaccgt ttggaagata ttgcttttct aaattcagga
    18061 tgcacgattt caactgcttc tgctagtatg atgacagatg ccgttttagg aaaaaccaaa
    18121 caagaaattt tagaactggc gactattttt tctgaaatgg ttcaagggca aaaagatgag
    18181 cgtcaagacc aacttggaga cgcggcattc ttgtcaggtg ttgccaaatt ccctcaaaga
    18241 atcaagtgtg caaccctagc ttggaatgcc cttaagaaaa caattgaaaa tcaagaaaaa
    18301 cagtaagaca agtttctttt gtcttatgaa ttattagaaa tgaagaaagg atattatggc
    18361 tgaagaaaga gtagaaccaa aaccaattga ccttggtgaa tataaatttg gtttccatga
    18421 cgatgtagag cctgtcttat cgacaggaaa aggactcaac gaaggtgtta ttcgtgaatt
    18481 atctgctgct aagggtgagc ctgagtggat gttggagttc cgtttgaagt cttatgaaac
    18541 cttcaaaaaa atgcccatgc aaacttgggg agcagacttg tcagagattg actttgatga
    18601 cttaatctac taccaaaaac catctgacaa accagcccgt tcttgggatg atgtacctga
    18661 aaagattaaa gaaacctttg aacgtatcgg gattccagaa gctgaacgtg cttatttagc
    18721 aggggcttct gcccagtacg agtcagaagt ggtttaccac aacatgaagg aagagttcca
    18781 aaaattaggt attatcttta cagatacaga ttccgcactc aaggaatacc cagacttatt
    18841 taaacaatac tttgcgaagt tggtaccgcc gacagataac aagttggcag ccctcaactc
    18901 agcagtatgg tcgggtggaa cttttatcta cgtgccaaaa ggtgtcaagg tagatattcc
    18961 acttcaaact tatttccgta tcaataacga aaatataggt cagttcgaac gtaccttgat
    19021 tatcgttgat gagggagcaa gcgtccacta cgtagaagga tgtacagcac caacatattc
    19081 aagcaatagc ttgcacgctg ccattgtaga aatttttgct ttggacggag cttatatgcg
    19141 ttatacaact atccaaaact ggtctgataa cgtctataac ttggtaacaa agcgtgctaa
    19201 ggctcaaaag gatgccactg ttgagtggat tgatggaaac ttgggtgcca aaacgactat
    19261 gaaatatcca tctgtttatc ttgatggaga aggagcgcgt ggtaccatgc tctctatcgc
    19321 ctttgctaat gcagggcaac accaagacac gggtgctaag atgattcaca atgctccaca
    19381 taccagctcg tctattgtgt ctaaatccat cgctaaaggt ggaggaaagg ttgactaccg
    19441 tggacaagtc acctttaaca agaactctaa gaaatctgtt tcccacattg aatgtgatac
    19501 cattatcatg gatgacttgt cagcatcaga tactattcca tttaatgaaa ttcacaactc
    19561 gcaagtggct ttggaacacg aagccaaagt atctaagatt tcagaagagc aattgtatta
    19621 tctcatgagc cgtggattgt cagaatctga ggcaactgaa atgattgtca tgggatttgt
    19681 agaacccttt acaaaagaac ttccaatgga atacgcagtt gagctgaacc gcttgattag
    19741 ctatgaaatg gagggatcag ttggataaaa tttgatttta tactcttcga aaatctcttc
    19801 aaaccacgtc agcatcgcct taccgtatgt atggttactg acttcgtcag tttcatctac
    19861 aacctcaaaa cagtgttttg agcaacctgc ggctagcttc ctagtttatt ctttgatttt
    19921 gagtattaga tttactcaaa atcaaggatt ttgaagatga acttgtatca aaaaatcgcg
    19981 gtttaaaatc gcgatttttt ataatttctc gttaacaaag cggacaaact gattccacca
    20041 aacttttaag aagaaggctt tttcaatttt cttgtctgct accatttcga aactagggcg
    20101 ctctgtggtg atgtaacctt gaccaatcaa gtccttgtct tcataagtca aatggccaac
    20161 cactgttcca gcttcaagtg gtgctgggat tgctttggaa tcaggtgtga attgaacaga
    20221 ttgggaagat tgattcccaa cacgttcgat tagatagata tcctctggag ccactgcagt
    20281 tactgtatct tcttttccat cttgtacagg ggctttgcta tcttgatagg catcgccttg
    20341 ttgaacgatt ttgcgaagtg taaatgtaga agaaatataa tccattaggg aagatgtagc
    20401 tgtaaatcga gcgtaaggat tattgtcttg atgatctgca tttaaaacaa ctgtgatgac
    20461 tctcatgcct ttttcgacag tagtaccaac aaaagactct ccagccttat ctgttgttcc
    20521 tgtttttagc ccatcaaaac caccacggta agcaggcata ccttctaaca tgtagttggt
    20581 tgaagtgatt gtcatcccag caaaagtaga agaaggtttt ttggtgattt ctaagacttg
    20641 tgggtatttt ttgatgaggt tgcgagcaac gatagcgaca tcataagcac taagcttatt
    20701 ttcctcatct tttttagaac ctgggtaaat gttatcccct agagtttcat tgttaagacc
    20761 tgtcgtattg acaacagtgg catcctgaat tccccattcc aagagttttg cccgcatcat
    20821 atcgatgaaa tctttttctg agccagcaat tttctcagct agggcaatag cggcgctgtt
    20881 ggcactagat accagagttg cttcaagcaa ctcttcgaca gtataattac gggcctccat
    20941 aggaatatta ctggcttcag aatttgtcgt caattgataa ggataatcag aaatatctac
    21001 aggagtggag agggtaatac ttccgttttc caaagcttca tagaccagat aagcagtaat
    21061 caattttgtt atggaagcaa tttcgacagg ttgcgttgca tccttctcat agagaatttt
    21121 accagtattt gcctcaacag caatcgcatg tttagcggca atggtaaaat cttgagcaac
    21181 agcagtagaa gcacccccta aaagagagac agttaacaaa gttaaaaata tttttttcat
    21241 agtagtctta ttctatcata aagaaaaaaa atattcttgc tttaataatt catctgttaa
    21301 gctttttgaa aatatggtaa aataaagtga gggaggtaac tcatgtttcg tagaaataaa
    21361 ttattttttt ggaccacaga aattttactc ttaaccatca tcttttacct atggagacag
    21421 atggggtctt tgattaaccc ttttgttagc gtgcttaata caattatgat tccattttta
    21481 ttagggggct ttctttatta tttgacaaac cctattgtta ctttcttaaa taaagtctgt
    21541 aaactcaatc gtttgcttgg tattttaatt accttgtgta ctttggtctg gggaatggtc
    21601 ataggtgttg tctatctctt acctattttg attaatcagt tatctagttt gattatatct
    21661 agtcaaacta tttatagtcg agtacaagac ttaatcatag acttatctaa ttatcctgcg
    21721 ctccagaatt tggatgtaga agctacaatt cagcagttaa acttatccta tgttgatatt
    21781 cttcaaaata tcctaaatag cgtatcaaat agtgtgggga gcgtcttgtc agctcttatc
    21841 agtactgttt tgattttgat tatgactcca gtttttttgg tttatttctt attagatgga
    21901 cataaattct tgcccatgct tgaaagaacg attctaaaga gggatcgctt gcatattgca
    21961 ggcttattaa agaatttaaa tgcgacgatt gctcgctata ttagtggagt ttcgattgac
    22021 gcaatcatta taggttgttt ggcttatatt ggctatagta ttattggttt aaaatatgct
    22081 ttagtttttg ccattttttc tggtgtagcc aatttaattc cttatgtggg gccaagtatt
    22141 ggtttgattc ctatgatcat cgcaaatata ttcactgatc cccatagact gctgattgca
    22201 gtgatttata tgcttgttgt tcagcaggta gatggcaata tcttatatcc tcgaatcgta
    22261 ggaagtgtta tgaaggttca tccaatcacg attttagttt tacttttgtt gtcaagcaat
    22321 atctatggtg tagttggaat gattgtcgca gtgccaacct attctatctt gaaagaaatt
    22381 tctaagttct tatcccgttt gtatgaaaat cataaaataa tgaaagaacg agaaagagaa
    22441 ttagctaagt aaaagtcagg agaaccctga tttttcttta ctggaagtgg cctttagatt
    22501 agaagactga aaataagtta aagtcttaaa ctaattttca cagctaagaa tagtagaagt
    22561 taatctgata aaaatcgaaa aaaccagtgg aattctgtgt cagggtaagt tccactggtt
    22621 ttcatagtct attaaagttc gaatgaaacc tatttatagt agattgaaac tagaatagta
    22681 cacctctaat tctaaaacat tgttagaaat cgatttgact gtcctgatct attcgttcta
    22741 ttcttatttc attttactat attttggtgc aataagtgaa aagtagtccg aataatataa
    22801 ggattgattt tatagttttt aaactcaaat gaattgaaat aaagagagta cgaaaattct
    22861 catctgaaag tattttagaa taattctctt cgtgaatttc ttcaaaacag atagcttcat
    22921 cttaggtatg tgatttcttt ttgcattttt gagttagata aggtataatg gttttattgt
    22981 cttttggggt cgttacggat tcgacaggca ttatgaggca tattttgcga ctcgtgtggc
    23041 gacgtaaacg ctcagttaaa tataactgca aaaaataaca cttcttacgc tctagctgcc
    23101 taaaaaccag caggcgtgac ccgatttgga ttgctcgtgt tcaatgacag gtcttattat
    23161 tagcgagata cgattaagcc ttgtctagcg gtttgataag agattgatag actcgcagtt
    23221 tctagacttg agttatgtgt cgaggggctg ttaaaataat acataaccta tggttgtaga
    23281 caaatatgtt ggcaggtgtt tggacgtggg ttcgactccc accggctcca ttattccttt
    23341 gcattctttt gctaaaaagg gagtcacaaa cagaccctat tttaaaaaaa ggatagaaaa
    23401 aaggatacaa catttgtcgc atcctaaaaa taatcttttt tcgacggaag acatgggatt
    23461 cgaacccacg cacgctatta cacgcctacc gcgtttccaa cacggcctct taagcctctt
    23521 gagtaatctt ccaatactta ctcaaatagt ctaccataaa ggctcttatc ttgcaataaa
    23581 aattctagaa ataagaaaaa tgatagattt tgaaagaaaa tgataaaaaa tgcttgactt
    23641 cgaaataaag tatggtagaa tgaatagtgt aaacgataac aggaggtgat tcagtgttaa
    23701 aaacagaacg taaacaactg attttagagg agttaaatca acatcatgta gtttctctag
    23761 aaaaattagt tagtttgcta gaaacgtcag aatcaacggt tcgaagagac ttggatgagt
    23821 tggaagcgga aaataagctt cgtcgtgtgc atggtggagc agaactcccc cactccttac
    23881 aggaagaaga aaccattcaa gaaaaatctg tcaaaaacct tcaagaaaag aaattgctgg
    23941 ctcagaaagc agcctctctc attaaagaaa aagatgtcat ctttatcgat gctggaacaa
    24001 caactgcttt tttgattcat gaattggtca ataagaatgt tacagttgtg accaactcca
    24061 ttcaccatgc cgctcagttg gttgaaaagc agattccaac tgtcatggtt ggaggaaacg
    24121 tcaagacggc gacagatgct agtatcgggg gcgttgctct taaccagatt aaccaattgc
    24181 actttgaccg tgcctttatc ggaatgaatg gtgttgacga tggctattat acgactcctg
    24241 atatggagga gggagctgtg aaaagagcta ttttggagaa tgccaagcag acctacgtct
    24301 tggtggattc gtcaaaaatt ggacaaactt gctttgccaa ggtagcccca ctcaaacgcg
    24361 ctatcgttat cactagtcaa gggcatgagc tcttgcaggt tattaaggag aaaacggagg
    24421 taatagaagt atgatttata cagtcacact caatccatcc attgactata tcgttcgttt
    24481 ggaccaagtc aaagttggta gtgtcaatcg tatggacagt gatgataagt ttgctggtgg
    24541 gaaaggaatc aatgtcagcc gtgtcttgaa acgtttgaat ataccaaata cagcgacggg
    24601 atttatcggt ggctttactg gtaaatttat cacagatact ttagcagagg aagaaatcga
    24661 gacacgtttt gtccaggtgg cagaagatac tcgtatcaat gttaaaatca aagcagacca
    24721 agaaacagaa atcaacggaa cgggtccaac tgttgaatcg gttcagctag aagaattgaa
    24781 agctatttta tctagtctga cagcagaaga tacagttgtc tttgcaggtt caagtgctaa
    24841 aaatctaggc aatgttatct ataaggattt gatttccttg acgcgccaga ctggtgcgca
    24901 agtggtctgt gactttgaag gacagacctt aattgatagt ttggactacc agcctcttct
    24961 tgtaaaacca aacaatcatg aacttggagc gatttttggg gttaaactcg aaagtttaga
    25021 tgaaattgag aaatacgctc gtgagttact ggctaagggt gctcaaaatg ttattatctc
    25081 tatggctggt gatggtgcct ttcttgtcac atctgaggga gcttacttcg ctaaaccaat
    25141 caaaggaaca gtcaaaaatt cagttggagc tggtgattct atggttgctg gattcacagg
    25201 tgaatttgtc aaatcaaaag acgtagtaga agccttcaaa tggggagtgg cttgcggaac
    25261 ggcaactacc ttctcaaatg acttggcaac ggcggaattt attaaagaaa catatggaaa
    25321 agttgaggta gaaaaacgat gaaaattcaa gacctattga gaaaagatgt catgttgcta
    25381 gatttgcagg caactgaaaa aacagctgtc atcgacgaga tgattaaaaa tttgacagac
    25441 cacggttatg taacagattt tgaaacattt aaagaaggaa ttttggcgcg tgaagctttg
    25501 acttctgctg gtttgggtga tggaatcgca atgcctcaca gcaaaaacgc tgctgtcaaa
    25561 gaagcgacag ttctatttgc taagtcaaat aagggtgttg actacgagag cttggatgga
    25621 caagcaactg acctcttctt catgattgca gctccagaag gtgccaatga tactcacttg
    25681 gcagccttgg cagaattgtc tcaatacttg atgaaagacg gttttgcaga caaacttcgt
    25741 caagcaacat ctgcagacca agttatcgaa ctttttgacc aagcttcaga aaaaactgag
    25801 gaacttgttc aagcacctgc taatgactct ggtgacttta tcgtagctgt tacagcttgt
    25861 acaacaggta ttgcccacac ttacatggcc caagaagccc ttcaaaaagt agctgctgaa
    25921 atgggggttg gtatcaaggt cgaaaccaac ggtgctagcg gtgttggaaa tcaactaact
    25981 gcagaagata tccgtaaggc taaagctatt atcattgcag cagacaaggc agttgaaatg
    26041 gatcgtttcg atggcaaacc tttgatcaat cgtccagttg ctgacggtat ccgtaagaca
    26101 gaagaattga tcaacttggc tatttcagga gatgcggaag tctaccgtgc cgctaatggt
    26161 gccaaagtcg caacagcctc taacgaaaaa caaagccttg gtggtgcctt ctacaaacac
    26221 ttgatgagtg gtgtatctca aatgttacca ttcgttatcg gtggtggtat catgattgcc
    26281 cttgccttct tgattgacgg tgctttgagt gttccaaatg aaaaccttgg caatcttggt
    26341 tcttaccatg agttagcttc tatgttcatg aaaattggtg gagctgcctt tggtttgatg
    26401 cttccagtct ttgcgggtta tgttgcctac tctattgctg aaaaaccagg tttggtagca
    26461 ggtttcgtgg ctggtgctat tgccaaagaa ggttttgcct ttggtaaaat tccttatgcc
    26521 gcaggtggtg aagcaacttc aactcttgca ggtgtctcat ctggtttcct aggtgccctt
    26581 gttggtggat ttatcgcagg tgccttggtt cttgccatca agaaatacgt taaagttcct
    26641 cgttcactcg aaggtgctaa atcaatcctt ctattgccac ttcttggaac aatcttgaca
    26701 ggatttgtta tgctagctgt gaatatccca atggcagcaa tcaacactgc tatgaatgac
    26761 ttcctaggcg gtcttggagg aggttcagct gtccttcttg gtatcgtcct tggtggaatg
    26821 atggctgttg acatgggtgg accagttaat aaagcagctt atgtctttgg tacaggtacg
    26881 cttgcagcaa ctgtttcttc aggtggttct gtagccatgg cagcagttat ggctggagga
    26941 atggtgccac cacttgcaat ctttgtcgca actcttcttt tcaaagataa atttactaag
    27001 gaagaacgta actctggttt gacaaacatc atcatgggct tgtcatttat cactgaggga
    27061 gcgattccat ttggtgccgc tgacccagct cgtgcgattc caagcttcat ccttggttca
    27121 gcagtagcag gtggactcgt tggtcttact ggtatcaaac tcatggcgcc acacggagga
    27181 atcttcgtta tcgcccttac ttcaaatgct ctcctttacc tcgtttctgt cttggtagga
    27241 gcaatcgtaa gtggtgtggt ttatggttac ctacgcaaac cacaagcata aaaaatagaa
    27301 aaatgaaaag attggaccgt ttggtgcagt ctttttctct ttccgaaatg cctgtgaaat
    27361 atggtataat agaagaatgg caaacaagaa tacaagtaca acaagacgga gaccgtctaa
    27421 agcagaactg gaaagaaaag aagcgattca acgaatgttg atttcgttag gaattgcgat
    27481 tttattgatt ttcgcagcct tcaaattagg ggctgcaggt ataacccttt ataatttaat
    27541 tcgcttgcta gtgggtagcc tagcttatct ggcgatattc ggcctattaa tctatctctt
    27601 ctttttcaag tggatacgaa aacaggaagg cctcttatct ggctttttca ccatatttgc
    27661 tggcttactc ttgatttttg aggcctactt ggtttggaaa tatggtttgg acaagtccgt
    27721 tctaaaaggg accatggctc aggttgtgac agatctgact ggttttcgaa cgactagctt
    27781 tgctggaggg ggtttgatcg gggtcgctct ttatattcca acagcctttc tcttttcaaa
    27841 tatcggaact tactttattg gttctatctt gattttagtg ggttctctcc tagtcagccc
    27901 ttggtctgtt tacgatattg ctgaattttt cagtagaggc tttgccaaat ggtgggaagg
    27961 gcacgagcgt cgaaaagagg aacgctttgt caaacaagaa gaaaaagctc gccaaaaggc
    28021 tgagaaagag gctagattag aacaagaaga gactgaaaaa gccttactcg atttgcctcc
    28081 tgttgatatg gaaacgggtg aaattctgac agaggaagct gttcaaaatc ttccacctat
    28141 tccagaagaa aagtgggtgg aaccagaaat catcctgcct caagctgaac ttaaattccc
    28201 tgaacaggaa gatgactcag atgacgaaga tgttcaggtc gatttttcag ccaaagaagc
    28261 ccttgaatac aaacttccaa gcttacaact ctttgcacca gataaaccaa aagatcagtc
    28321 taaagagaag aaaattgtca gagaaaatat caaaatctta gaagcaacct ttgctagctt
    28381 tggtattaag gtaacagttg aacgggccga aattgggcca tcagtgacca agtatgaagt
    28441 caagccggct gttggtgtaa gggtcaaccg catttccaat ctatcagatg acctcgctct
    28501 agccttggct gccaaagatg tccggattga agcaccaatc cctgggaaat ccctaatcgg
    28561 aattgaagtg cccaactccg atattgccac tgtatctttc cgagaactat gggaacaatc
    28621 gcaaacgaaa gcagaaaatt tcttggaaat tcctttaggg aaggctgtta atggaaccgc
    28681 aagagctttt gacctttcta aaatgcccca cttgctagtt gcaggttcaa cgggttcagg
    28741 gaagtcagta gcagttaacg gcattattgc tagcattctc atgaaggcga gaccagatca
    28801 agttaaattt atgatggtcg atcccaagat ggttgagtta tctgtttaca atgatattcc
    28861 ccacctcttg attccagtcg tgaccaatcc ccgcaaagcc agcaaggctc tgcaaaaggt
    28921 tgtggatgaa atggaaaacc gttatgaact ctttgccaag gtgggagttc ggaatattgc
    28981 aggttttaat gccaaggtag aagagttcaa ttcccagtct gagtacaagc aaattccgct
    29041 accattcatt gtcgtgattg tggatgagtt ggctgacctc atgatggtgg ccagcaagga
    29101 agtggaagat gctatcatcc gtcttgggca gaaggcgcgt gctgcaggta tccacatgat
    29161 tcttgcaact cagcgtccat ctgttgatgt catctctggt ttgattaagg ccaatgttcc
    29221 atctcgtgta gcatttgcgg tttcatcagg aacagactcc cgtacgattt tggatgaaaa
    29281 tggagcagaa aaacttcttg gtcgaggaga catgctcttt aaaccgattg atgaaaatca
    29341 tccagttcgt ctccaaggct cctttatctc ggatgacgat gttgagcgca ttgtgaactt
    29401 catcaagact caggcagatg cagactacga tgagagtttt gatccaggtg aggtttctga
    29461 aaatgaagga gaattttcgg atggagatgc tggtggtgat ccgctttttg aagaagctaa
    29521 gtctttggtt atcgaaacac agaaagccag tgcatccatg attcagcgtc gtttgtcagt
    29581 tggatttaac cgtgcgaccc gtctcatgga agagcttgag atggcaggtg tcattggtcc
    29641 agctgaaggt accaagccaa gaaaagtatt gcagcaataa aaaatagctt ctttccaagg
    29701 ttggaaggaa gctatttttc tggttattga ttacttttat tttctgaagt tggagtattg
    29761 ggctgttttt cattttcagt ggcaggttta cttgtagcag gtgtagtaga gtcctgagtt
    29821 gctgttttct gatcttcttt tttctcttcc ttgacgttag attttggcgt ttcttcttgt
    29881 tgtgtttttt cttgagaagt gttattttcc ttattgggag tagtattttc ctgaggggat
    29941 tctttttgaa tttctttgac aaaggttgtc gtctggcttg tcgtaggttc ttttttagta
    30001 tttttattat tatccaaggc gttcatgatc gtgatacccg cggtaatcaa accaaggata
    30061 ctaccgatag tagtaatggt tttttgaaaa acagtaaagc cttttttgat atgttctgtt
    30121 ttcggttttg aagttctacg atagttagac atacattctc tcctttgatt atgacttatt
    30181 atactctgtt tctttaaaaa aatcttaaaa aaagagaaat tgccctttct tgtcgcaagc
    30241 tccgtttcat gatacaatag agggagtgat tttagaaagc gtgagaaaca tgatttactt
    30301 tgataattca gcgacgacca agccttatcc tgaagccctt gaaacctata tgcaggtcgc
    30361 ttcaaaaatt ttaggaaacc catctagtct ccatcgtttg ggagaccaag caacacgaat
    30421 cttagatgct tctcgccaac agattgcaga tttaatcggt aagaaaagcg atgaaatctt
    30481 ctttacctct ggtggaacag aaggggataa ctgggttatc aagggtgtgg cctttgaaaa
    30541 agctcagttt ggcaagcaca tcattgtttc agccattgaa catccagcag tcaaagagtc
    30601 agccctctgg ttgaaaagtc aaggatttga agtggatttt gctccagttg ataagaaagg
    30661 cttggtcgat gttgaggcgt tagcaggttt gatacggcct gatacaatcc tcgtttccat
    30721 catggctgtg aacaatgaaa tcggctctat ccaacctatt gaggctattt cagaattctt
    30781 ggcagacaag ccgactattt ccttccacgt tgatgcggtt caggcgcttg ccaaaattcc
    30841 gactgaaaag tatctgacag aacgggtgga ttgcgcgacc ttctcgagtc ataaatttca
    30901 tggtgtccgt ggtgttggct ttgtctatat caaatctggc aagaagatta cacctcttct
    30961 tacaggtggt ggccaggagc gagattatcg ttcgacaact gaaaatgtgg cagggattgc
    31021 agcgacagcc aaggccctcc gtttatctat ggaaaagcta gatatcttta ggagcaagac
    31081 tgggcagatg aaggcagtga ttcgccaagc tcttctgaac tatccggata tttttgtctt
    31141 ttcagatgag gaagactttg cacctcatat tctgactttt ggaatcaaag gtgttcgagg
    31201 tgaagtcatc gttcacgcct ttgaagacta tgatattttc atctcaacaa cctcagcttg
    31261 ttcatctaag gcaggaaaac cagccggtac cttgattgcc atgggagtgg acaaagataa
    31321 ggccaagtca gctgtgcgtc ttagcctaga cttggaaaat gatatgagtc aggtcgagca
    31381 gtttttgacc aagttaaaat tgatttacaa tcaaactaga aaagtaagat aggagcattc
    31441 atgcagtatt cagaaattat gattcgctac ggagagttgt caaccaaggg taaaaaccgt
    31501 atgcgtttca tcaataaact tcgtaataat atttcggacg ttttgtctat ctatacccaa
    31561 gttaaggtaa cagcagatcg cgaccgtgcc cacgcttacc tcaatggagc tgattacaca
    31621 gcagttgcag aatctctcaa acaagttttt ggaattcaaa acttttctcc tgtttataag
    31681 gttgaaaaat ctgtagaagt tttgaagtct tctgtccaag agattatgcg ggacatctac
    31741 aaggaaggta tgacctttaa gatttctagc aagcgtagcg accacaactt tgaacttgat
    31801 agtcgtgaac tcaaccaaac acttggaggg gctgtattcg aagccattcc aaatgtgcaa
    31861 gttcaaatga aaagtcctga catcaatctt caggtggaga ttcgtgaaga agcagcctat
    31921 ctttcttatg aaaccattcg tggggctggt ggtttgccag ttggaacttc aggtaaaggg
    31981 atgctcatgt tgtcaggagg gattgactca cctgtagcag gttatcttgc tcttaagcgt
    32041 ggggtggata tcgaggcagt tcactttgct agtccaccat atactagtcc tggtgccctc
    32101 aagaaagcgc aggacttgac ccgtaaattg accaagtttg gcggaaatat ccagtttatc
    32161 gaggtgcctt tcacagagat tcaagaggaa atcaaagcca aagcgccaga agcttatttg
    32221 atgactctaa ctcgtcgctt tatgatgcgg attactgacc gtattcgtga ggtacgaaat
    32281 ggtttggtta tcatcaatgg ggaaagtcta ggtcaagtag ccagccaaac ccttgaaagt
    32341 atgaaggcta tcaatgctgt taccaacact cccatcattc gtcctgtggt taccatggac
    32401 aagttggaaa tcattgacat cgcccaggaa atcgatacct ttgacatttc aatccaaccg
    32461 tttgaagact gttgtaccat ttttgcacca gatcgtccaa aaacaaatcc taaaattaag
    32521 aatgcggagc agtacgaagc gcgtatggat gttgaaggct tggttgagcg agcagtggct
    32581 ggaatcatga ttactgaaat cacacctcaa gccgaaaaag atgaagttga tgacttgatt
    32641 gataatctac tctaattcag aaaatccaaa agaatagcga aaatcagtaa aaaaagttag
    32701 ttttttctct aaaaacaggt aaaaaactaa ctttttttct attttcatga tataatgata
    32761 taaaattttg aatatagaga gttttctgac aatgaatcaa tcctactttt atctaaaaat
    32821 gaaagaacac aaactcaagg ttccttatac agataaggag cgccgtgtac gtattcttct
    32881 tcctaaagat tatgagaaag atacagaccg ttcctatcct gttgtatact ttcatgacgg
    32941 gcaaaatgtt ttttatagca aagagtcttt cattggacat tcatggaaga ttatcccagc
    33001 tatcaaacga aatccggata tcagtcgcat gattgtcgtt gctattgaca atgatggtat
    33061 ggggcggatg aatgagtatg cggcttggaa gttccaaaaa tctcctatcc cagggcagca
    33121 gtttggtggt aagggtgtgg agtatgctga gtttgtcatg gaggtggtca agccgtttat
    33181 cgatgagacc tatcgtacaa aagcagactg ccagcatacg gctatgattg gttcctcact
    33241 aggaggcaat attacccagt ttatcggttt ggaataccaa gaccaaattg gttgcttggg
    33301 cgttttttca tttgcaaact ggctccacca agaagccttt aaccgctata tcgagtgcca
    33361 gaaactatcg cctgaccagc gcatcttcat ctatgtagga acagaagaag cagatgatac
    33421 agacaagacc ttgatggatg gcaatatcaa acaagcctat atcgactcgt cgctttgcta
    33481 ttaccatgat ttgatagcag ggggagtaca tctggataat cttgtgctaa aagttcagtc
    33541 tggtgccatc catagtgaaa tcccttggtc aaaaaatcta ccagattgtc tgagattttt
    33601 tgcagaaaaa tggtaagtta agaaaggaaa aaacgaaatg catattgaac atcttagcca
    33661 ctggagtggt catcctaacc gtgaaatgta ccttaaccgt tatggacatg gtgggattcc
    33721 agttgtggta tttgcttcat caggtggtag tcacaacgaa tactatgatt ttggcatgat
    33781 tgatgcctgt gcttccttta tcgaggaagg ccgtgtccag ttctttaccc tatctagttt
    33841 ggatagtgag agctggttgg ctacttggaa aaatgctcat gaccaagcgg aaatgcaccg
    33901 tgcctacgaa tgttatgtga ttgaggaggc cattctttta tcaagcacaa gtcaggttgg
    33961 tttgatggca tgatgacgac aggttgctct atgggagcct atcatgcact caatttcttc
    34021 ctccagcatc cagatgtctt taccaaagtg attgctctca gtggtgttta cgacgcacgt
    34081 ttctttgtcg gtgattacta caacgatgat gctatttacc aaaactcgcc agtagattat
    34141 atttggaacc aaaacgacgg ctggtttatt gaccgttacc gtcaggcaga gattgtgctg
    34201 tgtacggggc ttggagcctg ggaacaagat ggttttccat ccttttacaa gctcaaagaa
    34261 gcctttgaca agaaacaaat tccagcctgg tttgctgaat ggggacatga tgtcgcccat
    34321 gactgggaat ggtggcgtaa acaaatgcct tatttcctcg gtaatctcta tttataaaag
    34381 gagttaccta tgaattacct tgttatttct ccctactatc cacaaaactt tcaacagttt
    34441 accatcgaac tagctaataa aggcatcaca gtcttgggaa ttggtcaaga gtcttacgag
    34501 caattggatg agcccttacg caatagcttg accgagtatt ttcgtgttga taatcttgag
    34561 aacatagatg aagtcaaacg tgcagttgct tttctctttt ataaacatgg tccaattgac
    34621 cgcatcgagt ctcacaatga atactggctt gagctagacg caacactcag agaacaattc
    34681 aatgtttttg gtgccaaacc agaggatctc aaaaagacga aatataagtc tgaaatgaag
    34741 aaacttttca aaaaagcagg tgttcctgtg gtacctggag ctgttatcaa gacggaagca
    34801 gatgttgatc aagcagtgaa agaaatcggt cttccaatga ttgccaaacc tgataatgga
    34861 gtgggagcag ccgcaacctt taaacttgag acagaagacg atatcaatca cttcaagcaa
    34921 gaatgggacc attcaaccct ttatttcttt gaaaaatttg tcacttccag cgaaatctgt
    34981 acctttgacg ggctcgtgga caaggatgga aagattgtct tctcaacaac ctttgactac
    35041 gcctatacac cgcttgacct catgatttat aagatggaca attcttatta tgtgctcaag
    35101 gatatggatc ctaaactgcg caagtatggg gaagcaattg tcaaagaatt tggtatgaaa
    35161 gaacggtttt tccatattga gttcttccgt gagggggacg attatattac catcgagtac
    35221 aataaccgcc ctgcaggtgg ttttaccatt gatgtttata actttgctca ttccttggac
    35281 ctttatcgtg gctatgcagc tattgtcgca ggagaggagt tcccggcgtc agactttgaa
    35341 actcagtatt gtttggctac ttctcgccgt gcaaatgctc actatgttta ttcagaagag
    35401 gatttgcttg ccaaatatag ccagcagttc aaggttaaaa aagtcatgcc agctgccttc
    35461 gcggaacttc aaggagatta cctgtatatg ctgaccactc cgagtcgaca agaaatggat
    35521 cagatgattg ctgatttcgg acaacgtcaa gaataagaac tatcggatta aggaaattca
    35581 ctcccttaat ccttttgttt tgtctggtaa aaaataagag catcctaaca aggtagctat
    35641 cataaaactt gttcgataac tatttgaagc aggattaggt ggctagaaat tgaattttaa
    35701 catttcaatt tagtcatagt attgtgtttt cgtatcctta aatcagctaa aaggatccat
    35761 gacgacacct atacgatata gttttcagga taccaaacaa gtctattaat actcaatgaa
    35821 aatcaaagag caaactagga agctagccgc aggttgctca aaacactgtt ttgaggttgt
    35881 ggatagaact gacagagtca gtatcatata cctacggcaa ggtgaagctg acgtggtttg
    35941 aagagatttt cgaagagtat aaaatattca ggtgacgcat agatatagtt aattgaagct
    36001 ttgtttgaaa tctgataaaa taatgatatt actaagtttt aaaaactaaa gaaaagggaa
    36061 gatatgatta caggcgaatt aaaaaataaa atcgatcagc tgtgggaaat tctttggaca
    36121 gaaggaaacg caaatccttt aacaaatatt gaacagttga cttatctctt atttatgaaa
    36181 gatttggata gtgtcgagct tggacgtgaa agtgatgctg aatttctagg gattccttat
    36241 gagggagttt ttccaaaaga taaacctgaa taccgttggt caacttttaa aaatatagga
    36301 gatgctcagg aagtttatcg tttaatgact caggagattt ttccgtttat taaaaatctc
    36361 aagggggata cagatgatac agccttttca cgatatatgc gagaagctat ttttcaaata
    36421 aataaacctg ctacgcttca aaaggcaatt tctatcttag atgtttttcc aactagggga
    36481 ttagatgtag attttgataa tgacaaacaa agtattactg atatcggaga tatctatgaa
    36541 tatctgttat caaaattgtc gaccgcaggt aaaaatggac agttccgtac acctcgtcac
    36601 atcatcgata tgatggttga gttgatgcaa ccgactatca aagatatcat ctcagatccc
    36661 gctatgggtt ctgctggctt cttagtatct gctagccgtt acttaaagcg taagaaagat
    36721 gaatgggaaa ccaatacaga taatatcaat cattttcata atcagatgtt tcatggaaat
    36781 gatacggata cgactatgtt gagacttggg gcgatgaaca tgatgctaca tggagtagaa
    36841 aatccacaaa tcagttacct tgactcgctg tctcaagata atgaagaagc cgataaatat
    36901 actttggttt tagcaaatcc tccttttaag ggctcacttg actacaattc aacctctaat
    36961 gaccttcttg caaccgtaaa aaccaaaaaa acagaattac tctttctttc tcttttcttg
    37021 cgaactttaa aaccaggtgg acgagcagca gttatcgtac ctgatggtgt cctttttggt
    37081 tcgtctaaag ctcataaagg aattcgtcag gaaattgtag agaatcataa gcttgatgct
    37141 gtaatctcaa tgcctagtgg tgtgttcaag ccttatgctg gagtttcaac tgccattctc
    37201 atctttacaa aaactggtaa tggtggtact gacaaagtct ggttttacga tatgaaagcg
    37261 gatggtttaa gtttggatga taagcgacaa ccgattagcg acaatgatat tccagatatt
    37321 atcgaacgct ttcatcatct tgaaaaagaa gcagaacgtc agagaacgga tcaatctttc
    37381 tttgttccag ttgctgagat aaaggaaaat gattatgatt tgtctatcaa taaatataaa
    37441 gagattgagt atgaaaaagt tgagtatgaa ccaacagaag tcatcttaaa gaaaatcaat
    37501 gatttagaaa aagaaattca agctggcttg gctgaattgg aaaaattact caagtaggga
    37561 ggaggctgta tgaaaaaagt gaagttgggg gaaatcttat ctctaaaaaa aggaaagaaa
    37621 gccactgtac ttgctgaaca aacaactcta agccaacgtt atattcaaat agatgattta
    37681 agaaataata ataatttaaa attcactgaa agtttaaata tgactgaagc actcccagat
    37741 gatattctaa tagcatggga tggtgctaat gcaggaacag ttggctatgg gttatcggga
    37801 gctgttggta gtacaattac ggtcttaaaa aagaatgagc gatacaaaga aaaaattata
    37861 tcagattact tgggagtctt tttggaaagt aaatcgcagt atttacgaga tcattcaaca
    37921 ggtgcaacta ttcctcattt aaacaagaat atattacttg atttacaatt agaattgcta
    37981 ggtatcgaag aacaagagaa cattatctgt attcttaata cgattaaaag gcttattact
    38041 agaagaaaat ttcagttaga tgaactaaac ttgctcg
//
LOCUS       DAVBGY010000014        37591 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVBGY010000014 DAVBGY010000000
VERSION     DAVBGY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.014"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            51..299
                     /locus_tag="VQ620_000744"
     CDS             51..299
                     /locus_tag="VQ620_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763409.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QEFVDEMGRTYSGNLILQELITVNEAYKATYIDELSSN"
     gene            430..1602
                     /locus_tag="VQ620_000745"
     CDS             430..1602
                     /locus_tag="VQ620_000745"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HET2763410.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            1602..2876
                     /locus_tag="VQ620_000746"
     CDS             1602..2876
                     /locus_tag="VQ620_000746"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HET2763411.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVEQLIADKTFNLETAQRYLENLRS"
     gene            complement(2912..3877)
                     /locus_tag="VQ620_000747"
     CDS             complement(2912..3877)
                     /locus_tag="VQ620_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2763412.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(3858..5312)
                     /locus_tag="VQ620_000748"
     CDS             complement(3858..5312)
                     /locus_tag="VQ620_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET2763413.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLIHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            5689..7572
                     /locus_tag="VQ620_000749"
     CDS             5689..7572
                     /locus_tag="VQ620_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2763414.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEAAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFIIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTGDYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            7721..8608
                     /locus_tag="VQ620_000750"
     CDS             7721..8608
                     /locus_tag="VQ620_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2763415.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALDVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGENIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(8928..9545)
                     /locus_tag="VQ620_000751"
     CDS             complement(8928..9545)
                     /locus_tag="VQ620_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET2763416.1"
                     /translation="MSQDKQMKAASPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(9960..10148)
                     /locus_tag="VQ620_000752"
                     /pseudo
     CDS             complement(9960..10148)
                     /locus_tag="VQ620_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(10658..10738)
                     /locus_tag="VQ620_000753"
                     /pseudo
     CDS             complement(10658..10738)
                     /locus_tag="VQ620_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            10855..11748
                     /locus_tag="VQ620_000754"
     CDS             10855..11748
                     /locus_tag="VQ620_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2763417.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            11745..12944
                     /locus_tag="VQ620_000755"
     CDS             11745..12944
                     /locus_tag="VQ620_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2763418.1"
                     /translation="MRNMWVVIKETYLRHVKSWSFFFMVISPFLFLGISVGIGHIQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINDYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKIF
                     KRALSYK"
     gene            complement(13297..14064)
                     /locus_tag="VQ620_000756"
     CDS             complement(13297..14064)
                     /locus_tag="VQ620_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763419.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(14081..14776)
                     /locus_tag="VQ620_000757"
     CDS             complement(14081..14776)
                     /locus_tag="VQ620_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2763420.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            complement(14781..15146)
                     /locus_tag="VQ620_000758"
     CDS             complement(14781..15146)
                     /locus_tag="VQ620_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2763421.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            15286..15759
                     /gene="nrdR"
                     /locus_tag="VQ620_000759"
     CDS             15286..15759
                     /gene="nrdR"
                     /locus_tag="VQ620_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HET2763422.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            15760..16929
                     /locus_tag="VQ620_000760"
     CDS             15760..16929
                     /locus_tag="VQ620_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HET2763423.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLDHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSPKETTIIKEAKSKTALQFLAEIKQTRKGTTTQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            16930..17826
                     /gene="dnaI"
                     /locus_tag="VQ620_000761"
     CDS             16930..17826
                     /gene="dnaI"
                     /locus_tag="VQ620_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HET2763424.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            17823..18536
                     /locus_tag="VQ620_000762"
     CDS             17823..18536
                     /locus_tag="VQ620_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HET2763425.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYPVFGMAL
                     GVPNQTHDVKPRLPLDNVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            18550..19860
                     /gene="der"
                     /locus_tag="VQ620_000763"
     CDS             18550..19860
                     /gene="der"
                     /locus_tag="VQ620_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET2763426.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFIIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            19949..20284
                     /locus_tag="VQ620_000764"
     CDS             19949..20284
                     /locus_tag="VQ620_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763427.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            20424..20759
                     /locus_tag="VQ620_000765"
     CDS             20424..20759
                     /locus_tag="VQ620_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763428.1"
                     /translation="MEHLFKMVILLPCFYFFSWIEKANRDSKIFPILYYFYWFYIPLY
                     SLFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            20944..21735
                     /locus_tag="VQ620_000766"
     CDS             20944..21735
                     /locus_tag="VQ620_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763429.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLEWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLKKYEDNV"
     gene            21728..22369
                     /locus_tag="VQ620_000767"
     CDS             21728..22369
                     /locus_tag="VQ620_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763430.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLIVSRLEEKFKKG"
     gene            22373..23023
                     /locus_tag="VQ620_000768"
     CDS             22373..23023
                     /locus_tag="VQ620_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2763431.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            23034..23321
                     /locus_tag="VQ620_000769"
     CDS             23034..23321
                     /locus_tag="VQ620_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763432.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     MQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            23467..25980
                     /gene="secA"
                     /locus_tag="VQ620_000770"
     CDS             23467..25980
                     /gene="secA"
                     /locus_tag="VQ620_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET2763433.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMATLTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            26061..27092
                     /locus_tag="VQ620_000771"
     CDS             26061..27092
                     /locus_tag="VQ620_000771"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2763434.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            27094..28125
                     /locus_tag="VQ620_000772"
     CDS             27094..28125
                     /locus_tag="VQ620_000772"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2763435.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            28164..28532
                     /locus_tag="VQ620_000773"
     CDS             28164..28532
                     /locus_tag="VQ620_000773"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET2763436.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            28522..29625
                     /locus_tag="VQ620_000774"
     CDS             28522..29625
                     /locus_tag="VQ620_000774"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET2763437.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEAPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            29644..31659
                     /gene="recG"
                     /locus_tag="VQ620_000775"
     CDS             29644..31659
                     /gene="recG"
                     /locus_tag="VQ620_000775"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET2763438.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEIVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            31975..32955
                     /locus_tag="VQ620_000776"
     CDS             31975..32955
                     /locus_tag="VQ620_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HET2763439.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            33760..33987
                     /locus_tag="VQ620_000777"
     CDS             33760..33987
                     /locus_tag="VQ620_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763440.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            34275..37172
                     /gene="nanA"
                     /locus_tag="VQ620_000778"
     CDS             34275..37172
                     /gene="nanA"
                     /locus_tag="VQ620_000778"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HET2763441.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNNQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYGNYNDAPLKVKPGQWNSVTFTVEKPTPE
                     LPKGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIYGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT        11241 a         7015 c         8382 g        10953 t
ORIGIN      
        1 tactttcctt attgtctgta agtctactga ccttgttgtt tatcccagtc atggtttcta
       61 gttcgggctc agagtttcaa agtggatggc aagagcatca attgattgct gagaaggtta
      121 gtaaaacact tgacaagaca tttgataagg atgtcagaga aattccgacc agtcagtttt
      181 atcaagaatt tgtagatgag atgggaagga cttactcagg aaatttgatc ctccaggagc
      241 tgataactgt gaatgaagct tataaagcta cttatatcga tgaactttct agcaactaat
      301 cattctttaa aatgatgacc aataagaggc ttgatttggt gaatcaagtc ttttttctct
      361 cttttactgc catttgtgat aaaatagtat agaacgattt tttacatgaa tgataaaaca
      421 gaggtaaata tgacaatcgg tattgataag attggttttg cgaccagtca atatgtcttg
      481 aaattacaag acttagcaga agcgagggga attgaccctg aaaaattaag taaaggactc
      541 ttactcaagg aattgagtat tgcgccccta actgaggaca ttgtgacctt ggcggccagt
      601 gctagtgact ctattttaac tgagcaagaa agacaagaag ttgacatggt cattgtggct
      661 accgagtcag gaattgacca gagtaaggct gcggccgtct ttgtgcatgg cttgctgggc
      721 atccagccct ttgctcgtag tttcgagatt aaagaagcct gttatggagc gactgctgcc
      781 cttcattatg ccaaattgca tgtggaaaat tctccggagt ccaaggtctt ggtcattgcc
      841 agtgatattg ccaaatacgg tattgaaact ccaggagaac caactcaagg tgctggaagt
      901 gtagctatgt tgattacaca aaatccacgc atgatggcct ttaataatga caatgtagct
      961 cagacccgtg acatcatgga tttctggcga ccaaattact cgacaactcc ttatgtaaat
     1021 ggtgtttatt ctacccaaca atacttggat agtttgaaaa cgacttggct tgaatatcaa
     1081 aaacgctacc agcttactct ggatgatttt gcggctgttt gtttccactt gccttatcct
     1141 aaattagcgc taaaaggctt gaaaaaaatc atggataaga gcctgcctca agagaaaaaa
     1201 gacctcttac aaaagcattt tgaccagtct attctctaca gtcaaaaggt ggggaatatc
     1261 tacacaggtt cacttttcct tggacttttg tctctcttgg aaaatacaga tagcttgaaa
     1321 gctggggata aaatcgccct ttatagttac ggaagtggag ctgtggctga gttcttcagt
     1381 ggtgaattgg ttgaaggata tgaagcttat ttggataaag accgcttgaa caagctcaac
     1441 caacgaactg ccttatccgt tgcagactat gaaaaggtct tttttgagga agtaaacttg
     1501 gatgaaacaa actctgccca gtttgctggc tatgaaaatc aagattttgc cttggttgaa
     1561 attctcgacc accaacgccg ttatagcaag gttgaaaaat aatgaagata agttggaatg
     1621 gattttctaa aaaatcatac caagagcgcc tcgagctgtt aaaagctcag gcgctcctta
     1681 gtcctgagag acaagctagt ctggagaagg atgaacagat gagcgtgact gtggcagacc
     1741 agctgagtga gaatgtagtg ggaacttttt ctctgcctta ttcgctggtt ccggaggtac
     1801 ttgtcaacgg tcaggaatac accgttccct atgtgacaga agaaccctct gtggttgcag
     1861 cggccagcta tgccagcaaa atcatcaagc gtgcaggtgg ttttactgca caagtccatc
     1921 agcgccagat gattgggcag gtagcccttt atcaagttgc taatcctaaa ctagcgcaag
     1981 agaagattgc cagcaagaaa gcggagctct tggagcttgc caatcaagcc tatccttcta
     2041 tcgttaaacg tggaggtggg gcgcgtgatc tgcatgtcga gcagataaaa ggcgaaccag
     2101 actttctcgt tgtttatatt catgtcgata cccaggaagc catgggtgcc aatatgctca
     2161 acaccatgct ggaagccttg aaaccagtct tagaagaact cagtcaggga cagagtctca
     2221 tgggaatcct gtccaactac gcgaccgatt ctctggtgac tgcaagctgt cgcatcgcct
     2281 ttcgctactt gagccgccaa aaggatcaag gacgagagat tgcggagaaa attgcgttgg
     2341 ctagtcagtt tgcgcaggct gatccttacc gagctgctac tcataataaa ggaattttta
     2401 atggtattga tgcgattttg attgccactg gtaatgactg gcgtgccatc gaagctgggg
     2461 cccatgcctt tgccagtcga gatggacgtt atcaaggtct tagttgctgg acgctggacc
     2521 ttgaaagaga agaattggtc ggtgagatga ccctgcccat gcctgtagcg actaagggtg
     2581 gctctatcgg cctcaaccca cgtgtagctc tcagtcatga tctactagga aatccttctg
     2641 ccagagaatt agcccagatt atcgtgtcca tcggtcttgc ccaaaatttt gcagccctca
     2701 aagccttggt aagtacgggc atccagcaag gccacatgaa actacaggcc aaatccctag
     2761 ctctcctagc tggggctagt gaatctgaag ttgctcccct agtcgagcag cttatcgcag
     2821 ataaaacctt taacctagag acagcccagc gctatctcga aaatttaaga tcataaaaaa
     2881 ctcagacgaa tcggtctgag ttttcttgtg tttaaatact ttttcctggt aatagtgtaa
     2941 ctggtaagaa gtaaccagtt gtggcgactt ccttgccttc aatcttttgc aagagaagat
     3001 caatagtgag acaagcaatc tcttccaaag gttgcttgat agtagccaat tgagggtagt
     3061 aattttcgat aaagtaggtc ccatcatagc cgatgacctt gagctctttt gggacagaaa
     3121 tgcccaattc ttgagcgatt ttaatgacca gaatagctgt caaatcatcc gaagcaaaaa
     3181 tggcatctgg tttttcccgg gtcaagatat tcttgatttc catctctttt ctgacgggag
     3241 aaaagtcact ggaaacattg ataataggag cttttgggag tacggatgca aaaccagcgt
     3301 ggcgcagtcc ggttggcgaa ttggaattgt cattccctgt aatcatgatg atagactggg
     3361 cacctgtctt gaccaaggtt tgggcagcaa gaaccccacc agcatagttg tcagaggaga
     3421 cgacagggat gtctggcgat aggtttcggt caaaggaaat aatcggcgct gtcacacgat
     3481 tgtagtcttc gattcctagg ttgtgactac cagaaatgat gccgtccacc tgattggctt
     3541 ccaacatttc gatgtattca cgttccttct cagaatcatg ttcactgttg cagatgatgg
     3601 tcttgtaacc atttttgaag agttggtgtt ccaatttatc aatcaattct gcatagaaaa
     3661 cattggaaat attggggaaa atcaagccga ttaacttagc tgattttcct tgcagactac
     3721 gagccaggtt gttgggttta tagcccaatt ctcgcatggc ttcattgact ttttggatgg
     3781 ttttctcaga tagataccct tttttattga taacccgaga aacggtagta ggactgacgc
     3841 ctgcaagttt ggcgacatca gttagttttg cgaccataat ctaattcata gtaagttcca
     3901 gttgggtttc cagatttgat caggatacca ttttggtccg catgtgggaa gacacgacca
     3961 gaaaatactt tttctccttt attgatgaaa atttcaaaga cagagttatc gatgaagatt
     4021 gtagcagtag tagcctgatt ctcgataggg caagagcgag ttgtcccaaa ttcttgggca
     4081 tactgttctc cagcctggct acgatccact gtcacttgac cgtttacaag gtcaaagttg
     4141 attgaaagtc ccttaccttc tttatcagca agtaagacaa tctcgctctg gctattagct
     4201 tccaagttga gttcaagttc gtaagtgttc ttggtttggg aacggtttga gaaggcttct
     4261 tcagaagcac gaaggtcctt aatagcagcg actggatact ggtagagctt gtcgtctttg
     4321 atagtgagtt ccttgaccaa agagaaggtt ccttggtggt caaaacggtc agatgggtaa
     4381 gaaacatctg gcaaaccaag ccagctaact gctagagcac gcccatcagg agcgttgaag
     4441 gcttgagttg cataggcttc gaaaccgtaa tccatgtttt gaagttgaga cacatctacc
     4501 attttggcat ttttagggtc aaaggaagcc ccaatcttat acatatttgg aaagatatta
     4561 tcgtagtcta gaactttctt atccaatcct tgtggacagt agagaaggac aggctgttcc
     4621 tctacaaaga ccagattagg acattccatc atgtaggcag tacggtcgtt agcaaagtca
     4681 aggtcgccaa ctgcttgcca gtttgtgtag tcgttattga cagccttgta gagacggacg
     4741 aagccttttt tctccaagtc ctgtccaccg acgacagcat agtattgacc tttaaagtta
     4801 aaaatttgtg gatcgcggaa gtggtcagta gagtctgctg gctggtcaat caagatcttg
     4861 tcaatctttg taatcttacc ctccttgtcc atcaaagcac cgatttggta tgggtgacgg
     4921 atccagtttt catcgcgaac atttcctgta taaaatagga ataagttatc gccaaattgc
     4981 atggcagaac cagagtaggc accgtggcta tctaatggag tatctggtaa aactttgatt
     5041 ccagtttctt taaagtgaat caaatcatca ctttctagct gtgcccaaga ttttaaaccg
     5101 tgggctgcac caaaaggaaa attctggtaa aagaggatcc acttgccatc aaagtaagaa
     5161 aagccatttg ggtcgttgag aagtcctgtt tttggctcaa catggtaatg agtatgccat
     5221 ggagattgtg ccatattttc ctttatatgt tgaatttctt cttgcgtcca atcttgataa
     5281 agtctgtaac gacgctcagt tgtccattcc attcattcct ccaaaaatct tatcactttt
     5341 aatagtaact gttttcgata aaaaatgcaa gccttttatg ttaaaaaaga gaaaaaactg
     5401 tcaaacgatt atcataaaat aaatacagga aatcaatcaa ttttttacga aaatgtgaaa
     5461 gataatgaaa taaaaaactt tttaaccaag gtttcaaact tatttttcaa ggaattcctg
     5521 atgaaacagt caaaaacaat caatctaaag cttgcccatt aagtggatag aatatttttt
     5581 ctgcaaaacg cttgacatat ttttaaaaca tgataaaata atagtcgtaa gggcgaataa
     5641 aatcgctttc aaacaagaac aaaatgttat ataaggagat ttttgcaaat gaacaatcag
     5701 gaaattgcaa aaaaagtcat cgatgccttg ggcggacgtg aaaatgtcaa tagtgttgcc
     5761 cactgtgcga ctcgtctacg tgtcatggtc aaagatgaag agaaaatcaa taaagaagtg
     5821 attgagaact tggaaaaagt tcaaggtgct ttctttaact cagggcaata ccaaattatc
     5881 tttggtacag gtacagttaa caaaatgtac gatgaagttg ttgtacttgg cttaccaaca
     5941 tcatctaagg atgacatgaa agcagaagct gctaaacaag ggaactggtt ccaacgtgct
     6001 atccgtactt tcggtgatgt tttcgttcca atcatcccag ttatcgtagc gacaggtctc
     6061 ttcatgggtg tgcgtggtct tttcaacgct cttgaaatgc cacttccagg tgactttgca
     6121 acttacacac aaatcttgac agatacagcc ttcatcatct tgccaggttt ggttgtgtgg
     6181 tcaaccttcc gtgtatttgg tggaaatcct gccgttggta tcgttcttgg tatgatgctt
     6241 gtctctggct cgcttccaaa cgcttgggca gttgctcaag gtggtgaagt aacagcaatg
     6301 aacttctttg gtttcatccc tgttgttggt ttgcaaggtt ccgttcttcc agccttcatc
     6361 atcggggttg tcggagctaa atttgaaaaa gctgtccgca aggttgttcc agatgtcatt
     6421 gacctcttgg taacaccatt cgtgacactt ttggtcatgt ctatccttgg actctttatc
     6481 attggaccag ttttccacgt tgttgaaaac tacatcctta ttgctacaaa agcgattctt
     6541 agcatgccat ttggtcttgg tggtttcttg attggtgggg ttcaccaatt gatcgtcgtg
     6601 tcaggtgtgc accacatctt caacttgctt gaagtgcaat tacttgctgc tgaccatgct
     6661 aacccattca acgctatcat cacagctgct atgacagctc aaggtgctgc tactgttgcg
     6721 gttggtgtta aaacaaaaaa tccaaaactg aaaacacttg ctttcccggc tgctctttct
     6781 gccttcctag gtattacaga gcctgctatc ttcggggtga acttgcgctt ccgtaaacca
     6841 ttcttccttt cattgattgc tggtgcaatc ggtggtggat tggcttctat ccttggactt
     6901 gctggtactg gtaatggtat caccatcatc cctggtacaa tgctttatgt tggtaacgga
     6961 caacttccac aataccttct tatggtagct gtatcatttg cccttggttt tgctcttact
     7021 tacatgtttg gttacgaaga tgaagtagac gcaactgcag ctgcaaaaca agctgaagtg
     7081 gctgaagaaa aagaagaagt tgcgccagca gctcttcaaa atgaaacact tgtaactcct
     7141 atcgttggtg atgttgtcgc tcttgctgat gtcaatgacc cagtcttctc aagtggagct
     7201 atgggacaag gtatcgctgt gaaaccaagc caaggcgtgg tctatgcacc agctgatgct
     7261 gaagtttcaa ttgcctttcc aacagggcac gcttttggtt tgaaaacaag aaatggtgct
     7321 gaagttttga ttcacgttgg tattgacact gtatctatga acggtgacgg ttttgaagca
     7381 aaagttgctc aaggtaataa ggtgaaagct ggcgatgttc ttggaacatt tgactcaaac
     7441 aaaatcgctg cagctggact tgatgataca acaatggtta tcgttacaaa tacaggtgac
     7501 tacgcttcag tagctccagt cgcaacaggt tcagttgcta agggggatgc tgtgatcgaa
     7561 gtgaaaatct aatcaatcct ctctaatgtg aaaacgaaca aatgacatgt ttgttcgttt
     7621 ttattagata gtaagattta cagaggaaaa tctaaaaaat agagacattt agactttcga
     7681 agtatgctat aataaagaaa ataaaaacaa gaggtttatc atgacaaaat tatatggaag
     7741 cttggaagcg ggcggtacaa agtttgtctg tgctgtcggt gatgaaaact ttaacgttgt
     7801 agaaaaaaca caatttccaa caacaactcc aatcgaaaca atcgataaaa ccattgagtt
     7861 cttctcaaaa ttcgataacc ttgctggtct tgcagttggt tcatttgggc cgattgatat
     7921 tgacaaaaac tcaaaaactt atggctttat cacgacgact ccaaaaccaa actgggcaaa
     7981 tgtggacttg cttggtgccc ttcgtcgcgc cctcgacgtg ccaatgtact tcacaacaga
     8041 cgtaaacagc tctgcttacg gtgaagtggt tgcccgtaac aatgctggtg gtcgtatcga
     8101 aaacttggtt tactacacaa tcggtacagg tatcggtgca ggcgtcatcc aacgtggtga
     8161 gtttatcggt ggtgtgggtc accctgaaat gggtcattat tatgttgcta gacacccaat
     8221 ggatattgaa aaagagttta agggtgtttg tcctttccat aagggatgtc tagaaggtta
     8281 tgcagctggt ccaagtttgg aagctcgtac aggtgtacgt ggggaaaata ttgaactcaa
     8341 caaccctgtt tgggatgttc aagcctacta tatcgctcaa gctgcggtta atgcgacagt
     8401 gactttccgc ccagacgtga ttgtctttgg tggaggggtc atggctcaac aacatatgct
     8461 ggaccgtgtc cgtgagaaat ttacatctct tcttaatggt tacctaccag taccagatgt
     8521 gcgtgactat atcgtgacgc cagcagtcgc aggaaatggt tctgccacac ttgggaactt
     8581 tgttcttgca aaagaagttt caaaataaag cacaaaatcc gcttgggaaa ccaaacggat
     8641 tttgtggttg aaaacaatat ttttgagcct ttccttaagt gattacggac ggtagcgact
     8701 ttcttcgaaa ttccatacct aaactttgag cctagtgtct caaagtttcc gaatacctga
     8761 aaccattaga tttcaggtgt ttctatcacg gcggaaaggc taggtaagtc ctcgcctcac
     8821 tatcgaaaaa tatgcataat ttccatacca aagtatattt agtatcaagc tatagttaat
     8881 ctacgcttta aaaatccact tgggaaacca aacggatttt ttacgtgtca aagcatttgc
     8941 cagaaaaagt caataatata ggtcagaccg taggtataaa ccacgagggt aaagggtttg
     9001 gtcagaatga tgatggtcat gtagcgcttg aaactcatct tggtcagggc agccagcata
     9061 cagagaaagt cagctgggct aatgggccaa atcatcataa aaataaagaa gcggtcaaaa
     9121 cgattgccct tatctagcca gccgatgtac ttgtcgtagg tgcgcttgct gacgacagac
     9181 tggacaaagg cagctccgta taggcgcacc agataaaaga taatggcaca gccaatcacg
     9241 atgccgatat agttgtagat agtcccgatg atgtgcccgt agataaagac cccagccacc
     9301 gaggtcaagg cccctggaat gatagggacg acagtctgta aaatctgtaa aaagataaag
     9361 agaggtggac cccagatgcc tgcctgctgg ataaaggcag agagggtttc cttggattgt
     9421 aaaatcccag cctgataagc ccaaatacag aaaatcaaac tcccaacccc accgacaatc
     9481 gatgagatat tgataactcg ctgcagaagg ggagaagcag ctttcatttg tttatcctgt
     9541 gacatgtaaa ctcctcatag atagtatatt tctactatac catattttgg aggagaaagg
     9601 gggaatgagt cttttttgat aaggaataga aagtaaatag cccttcgaga cttagaatag
     9661 gtctgtcaac taaaggttat atgcattcta ggtttcgaat aatcgcaatt ccataaattt
     9721 aaaagcattc taagtttcga atagcttatc tgagataatt ttgaggcatt cgcgctttcg
     9781 aatagtcatt ttccctaaag tttagatgta ttctgaagct agaaagacta aaatataaaa
     9841 gtagtaagag tctctctctg atggacgtaa tttatggcta gtatgaatcc tttgaattgt
     9901 tagttaattt taatattaat cagttctgaa ataattacta aatatagtta gatggtgaat
     9961 taattattgc taaatttcaa gttcaagtta gtcatccaga agtcttctct gggggacttg
    10021 tagttcaagc attttttagg atagttgtta atccactttt cgataaatgc gacttccttt
    10081 gtagtcgttt tcttagttcc ctgaggtaac catctatgaa tgagtctgtt gtgattttca
    10141 ttagatccta tagtgatgtg cataatagat atagcagatt gaaactagaa tagtatacat
    10201 ctacttctaa aacattgtta gaaatcaatt tgactgtcct gatcaatttg tcctgttttt
    10261 atttcatttt actatataat ccttagaaaa catatgggac aagcggttga attctgttcc
    10321 gtccattagt agataaagga ttactccttc ttgaaatgac agatgaactt gtttcttata
    10381 aaaatgttgt ctgaaactat tcgccttttt ccagaagttt tagaggaaga aaactttatg
    10441 aggaaaaagg agttactcaa ataacaattc aggattaaaa atagacagtt gaggagcgga
    10501 aggtataaat taagtttgct actgtataat ggatttatca ctaatactct tcgaaaatct
    10561 cttcaaacca cgtcagcttc gccttgccgt agatatagtt actgacttcg tcagttctat
    10621 ccacaacctc aaaacagtgt tttgagctga cttcgtcagt tttatctgca acctcaaagc
    10681 tgtactttga gcagcctacg gctagctttc tagtttgctc tttgcttttc attgagtata
    10741 aaagtgctcc aggccatatt ttacacagca atagtcttct aaaaatggac aaaaaacatt
    10801 gtaaaggcta tcaaaaagga gtataatgag tgcaagaaat ttcggaggtg aaagatgcta
    10861 gaagtaagaa gtctagagaa aagttttgga tccaagcaag ttttgtttgg tattgacttt
    10921 caagcgcgac caggtcgtat tttgggacta gtcggaaaaa atggtgctgg aaaaacaacg
    10981 attttccata gtattttgaa attcctagaa tatcagggag aaatcggtct ggatggtcag
    11041 gatattcgtc aggagaccta tgctcggatt ggttatctgc ctgaagaacg cagtctcatg
    11101 cctaaattga cagttcttga acaagttcgc tacttggcga ctctaaaagg catggatgcc
    11161 aaagaagtca aagaaaaact ccctcaatgg atgaagaggt tggaagtgaa aggaaagctg
    11221 acagataaaa tcaagagtct gtcaaaagga aatcagcaga agattcagct cattattact
    11281 ctgattcatg aaccagacct gattatcttg gatgagcctt ttagtggatt ggacccagtt
    11341 aatacagaat tgctcaaaca agtcattttt caggaaaaag agcgcggagc aaccattatc
    11401 ttttctgacc atgtcatgac caatgttgag gaactttgtg acgatattct gatgatccga
    11461 gatggccgtg tggtcttgca cggaccagtt caggatgtcc gcaatcaata cgggaaaacg
    11521 cgtctctttg tttcaagtga acgaagcaag gaagaattgg aaaatcttcc tcatgtcaaa
    11581 caggtgagct tgaccaaaca aggcagttgg aaattgattt tggaggatga gagcgctgga
    11641 agggaactct tcccaatctt gactcaaggt caatatatcg caacatttga ccaacaagcg
    11701 ccaactatcg atgaaatctt taaactagaa tcaggagtgg aagtatgaga aatatgtggg
    11761 ttgtaatcaa ggaaacctat cttcgacatg tcaagtcatg gagtttcttc tttatggtga
    11821 tttcgccgtt cctcttttta ggaatctctg taggaattgg gcatatccaa ggttcttcta
    11881 tggctaaaaa taataaagtg gcagtagtga caacagtgcc atctgtagca gaaggactga
    11941 agaatgtaaa tggtgttaac ttcgactata aagacgaagc aagtgccaaa gaagcaatta
    12001 aagaagaaaa attaaaaggt tatttgacta ttgatcaaga agatagtgtt ctaaaggcag
    12061 tttatcatgg cgaaacatcg cttgaaaatg gaattaaatt tgaggttaca ggtacactca
    12121 atgaactgca aaatcagctt aatcgttcaa ctgcttcctt gtctcaagag caggaaaaac
    12181 gcttagcgca gacaattcaa ttcacagaaa agattgatga agccaaggaa aataaaaagt
    12241 ttattcaaac aattgcagca ggtgccttag gattctttct ttatatgatt ctgattacct
    12301 atgcgggtgt aacagctcag gaagttgcca gtgaaaaagg caccaaaatt atggaagtcg
    12361 ttttttctag cataagggca agtcactatt tctatgcgcg gatgatggct ctgtttctag
    12421 tgattttaac gcatattggg atctatgttg taggtggtct ggctgccgtt ttgctcttta
    12481 aagatttgcc attcttggct cagtctggta ttttggatca cttgggagat gctatctcac
    12541 tgaatacctt gctctttatt ttgatcagtc ttttcatgta cgtagtcttg gcagccttcc
    12601 taggatctat ggtttctcgt cctgaggact cagggaaagc cttgtcgcct ttgatgattt
    12661 tgattatggg tggttttttt ggagtgacag ctctaggtgc agctggtgac aatctcctct
    12721 tgaagattgg ttcttatatt ccctttattt cgaccttctt tatgccgttt cgaacgatta
    12781 atgactatgc ggggggagca gaagcatgga tttcacttgc tattacagtg atttttgcgg
    12841 tggtagcaac aggatttatc ggacgcatgt atgctagtct cgttcttcaa acggatgatt
    12901 tagggatttg gaaaatcttt aaacgtgcct tatcttataa atagaagagc cttgcgaaag
    12961 cgaggttttt aaaggtgaaa agggtggaat cacgaaccta attatgtaca cattatgtaa
    13021 acaagttgct tgaaaactca attgtatagt gaactgaatc tggaatagga taatattact
    13081 tctaaaacat tcttataaat tgatttgaat tccaaaatcc catcgttccg ttcttatttc
    13141 attttgcgat atagtgaaag gaaataagtc agctcacaaa aaacgcataa tatcaaggat
    13201 ttttaacact tgatactatg cgttttctaa ttttaaagac tttttgcctg gtctcttttg
    13261 ttctatgagt atgttacata tctaggtaaa aattatttaa agcaaattaa ccttatttct
    13321 caagatgtag taggttccga gataaaagat agctatgaat atgagttctt taacaatggc
    13381 tatacctgct cccatataga aatggtcatt gagtcctacc agtgaattga catagaaatt
    13441 agaactaaga ttgaagaaaa gttcaataaa tccaatgacg atttggatac caatgtaggc
    13501 tgcaacagcg agtgctgtac ggtattcatt gaaaagctgt ccaatggaaa tagccaggta
    13561 gatgcagagg attcctgaaa tggtatttag taggaaggat atacctgtaa gaaagatctg
    13621 agggagatgt gtttctacaa atgtaatcac ataagaaaga ggaatccatt ctggagctgt
    13681 taaagccaga ataataacag cacttagagc caatacagcg gtgctaatca atgaccagat
    13741 aaaggcaccg attagtttgg ctgtgatgat atggtgttca gaaactggca aggtcaaagt
    13801 cagatagcct tgtcggtcgt agacactacc tttgaagcgt ttaataatca agaaaatggt
    13861 tgaaatcgca agtgtaagca tcaagccacc aaagactgta gctagaaaaa gtagcatagt
    13921 agcctgactt tcttggtaag gtagattttt aaagccttgt gtctgtattc cgatgagggc
    13981 agaaaggact agcacggctg cgtagagggc taaataccac ttgttaacat ttttaaattc
    14041 gtagtgaact aaattccaaa acataaataa tctcctttgc ttaggcctta aattcctgac
    14101 ggaagagttg gtcaatggat tcacctgact cgtagcgaat atcatctaca tttccttgac
    14161 ggacgacttt tccgtctttt aggaagacaa tttcatccaa gattggctcg atatcagaaa
    14221 tcaagtgggt agaaatcaaa acggtagaag ttggtgagta gttgttgata atggtattga
    14281 ggatataagc acgggctgct ggatccaccc caccaatggg ttcatccaaa acatagagac
    14341 gagcatcacg gctcataacc aaaatcagtt gaaccttttc tttgtttcct tttgatagtt
    14401 tcttgagacg actattttca tcaatgccca ggtctgcaag tagatgatga gcgcgttcaa
    14461 gattgaaatc tttatagaag gtcttgaagt aggttagggc ttctttgacc ttcatttgct
    14521 cattgagata ggtcgtatca ggcaaataag ctacaacggc cttggttgct gggcttgggt
    14581 ccatgtcgtt gatgaggaca cgtccttgat ctggttgtaa gaggccatta attagtttaa
    14641 tcagggttgt ttttcctgag ccgtttggcc caagaaggcc gacaattttt ccagctggaa
    14701 tgtcaagaga aacattttca agggctggtg ttgctccata tgatttggat acattttcaa
    14761 atgctagtaa tgacataggc ttaaactcct ttaatataat cactgactac gcctggtagt
    14821 tcttcttttt catagccaaa atgggtcatg gaggaaacga agtgttccaa ttcttcttct
    14881 gataattgtt tgcgtgactg agcgattagc tccttatcct tagtcacaaa tcgtccagtt
    14941 gttcgcttgc tgtagacaaa tccttctcgt tcaaggtctg ataaggctct ttggatggta
    15001 ttgggattga caccagcctc gctagctagc tccctcacgg ttggaagttg ttgattgggt
    15061 tccagtgtat gggaaacaat ctgaagcttg attttctcca taatctgtaa atagatgggt
    15121 tttttgttgt caaatgtcca ggacatctta gtctcctttc tcttattctt ttgtcttaat
    15181 taaataatac aacaaaacaa aaaacttgtc aagcaaaaag aagaattgtt ttgggaatcg
    15241 cttaaaaaat ggtataatga aaagaaaacg ataaggaggg aaaggatgcg ttgtccaaaa
    15301 tgtggggcta ccaagtcaag tgttatcgat agtcgccaag cagaagaagg gaacaccatt
    15361 cgtagaagac gagagtgcga cgagtgccaa caccgtttta caacctacga acgagtagaa
    15421 gaaagaacct tagtggttgt taaaaaagat ggcacacggg aacaattctc cagagataaa
    15481 atctttaatg ggattatccg ctcagcccag aaacgtcctg tgtcaagtga tgaaatcaac
    15541 atggtagtca atcgtatcga acagaaactc cgtggtcgaa atgaaaatga aattcaaagt
    15601 gaggacattg gttcactcgt catggaggag ttggctgaat tggacgagat tacctatgta
    15661 cgttttgcta gtgtctatcg tagttttaag gatgtcagtg agttagagag cttgctccaa
    15721 caaatcaccc agtcctctaa aaagaaaaag gaaagataaa tgaagccaat tgaccgtttt
    15781 tcttatctaa agaataatcg ggtgtcgcaa gatacctcat ctctggtaca gtgctacctc
    15841 ccgattatcg gtcaggaggc actgagcctt tatctttata caatcagttt ttgggataat
    15901 ggtagaaagg aatatctttt ttcaagtatc ctcaatcatc tcaactttgg gatggataga
    15961 ctgataaaat cattgaaaat cttatctgct tttaatctct tgaccctcta tcaaaagggg
    16021 gatgtttatc agctagccct ccatgctcct ctatctagtc aagacttctt ggagcatcct
    16081 gtttatcgca gactcttaga gaaaaagatt ggcgactctg ctgtggagga tttgaaagtt
    16141 gaaagtgctg atggtgaaga aatacctgtc tcactcaatc aagtctttcc agaattggca
    16201 gaactaggca gtcaagaaga ccttggtctc aagaagaaag tggccaatga ttttgacttg
    16261 gatcattttc gtcagctgat ggctcgagat ggacttcgtt ttgcggatga gcagtctgat
    16321 gtcttgaact tgtttgctat tgctgaggag aagaagtgga cttggtttga gacctatcaa
    16381 ttggccaagt caacagctgt ttcccaggtt atttcaacca aacgcatgcg ggaaaaaatt
    16441 gctcaaaaac cagtttcctc ggactttagt cctaaggaaa caaccattat caaggaagct
    16501 aaaagtaaaa ctgccctgca gttcttggca gaaatcaagc aaacacgcaa gggaaccact
    16561 acccaaacag aaagagaact cttgcaacag atggctggct tgggcttgct ggacgaagtc
    16621 atcaatatca ttcttttatt gacctttaat aaggtcgatt cggcaaatat caatgagaaa
    16681 tatgccatga aggtagccaa tgactatgcc tatcaaaaga ttcattcggc agaagaggca
    16741 gtcttgcgca tccgtgagcg aggacagaaa gcaaaaacac aaaaacagaa tcagactgcc
    16801 ccagtaaaaa ccaatgttcc taaatggagt aatcctgaat ataagaatga aaccagcgag
    16861 gaaactcgtc tggaactaga acgtaagaag caagaactat tagctcgatt agaaaaagga
    16921 ggagattaga tggaaagtgt cggagacgta ctcaaacgtc aacccagccg tttttattat
    16981 caagatttgg tccagaaaat catgaaggac cctgatgttg cggcttttat ccagcaagaa
    17041 tccttgactc cagaggaatt aaatcgcagt atctccaagt ttaatcagta catcaccgag
    17101 cgtgacaagt ttctccgtgg ggatacggat tatattgcca aaggctacaa gccgattttg
    17161 gttaagaatc atggctatgc agatgtttca tatgaagaaa ctcctgagct aatcgcggct
    17221 gaaaaagaag cggctattaa gaaccgtctc aagttaatca atctaccagc cagtctcaag
    17281 aaagctagtt tggctcaagt tgacttggat gatttggggc gcttaccagt ttttgaaaag
    17341 ctactagcct tcgtggagca atatccagct attcgaaaag gtctttactt atatggagac
    17401 tttggtgtgg gtaaaagttt catggtggcg gccttagctc atgatttatc agaaaaacgt
    17461 ggtgtttcat caactctcct ccactatcct agctttgtca ttgatgtcaa aaatgctatc
    17521 agtgatggca atgttaagac cttggtggat gagattaagc tgtctgaggt cctgatttta
    17581 gatgatattg gcgccgagca atcaaccgtt tgggtgcgtg acgaaatcct gcaggtcatt
    17641 ctccaatatc ggatgcagga aaatttaccg acctttttca catccaactt caactttgaa
    17701 gatttggaga agcattttgc gaaagtgaaa catggaaatg acgaaacctg ggaagccaga
    17761 cgggtcatgg aacgcatccg ttatttggct gaggagactc gtttagaagg agtaaaccgt
    17821 cgatgacaga aaccattaaa ttgatgaagg ctcatacttc agtgcgcagg tttaaagagc
    17881 aagaaattcc ccaagtagac ttaaatgaga ttttgacagc agcccagatg gcatcatctt
    17941 ggaagaattt ccaatcctac tctgtgattg tggtacgaag tcaagagaag aaagatgcct
    18001 tgtatgaatt ggtacctcaa gaagccattc gccagtctgc tgttttcctt ctctttgtcg
    18061 gagatttgaa ccgagcagaa aagggagccc gacttcatac cgacaccttc caaccccaag
    18121 gtgtagaagg tctcttgatt agttcggtgg atgcggcttt ggctggacaa aatgccctgc
    18181 tggcagctga aagtttgggt tatggtggtg tgattattgg cttggtacgg tacaagtcag
    18241 aagaagtggc agaactcttt aacctgcctg actacaccta tcctgtcttt gggatggcac
    18301 taggtgttcc aaatcaaact catgatgtga aaccaagact gccactagat aatgtagtct
    18361 ttgaggaaga ataccaagaa cagtcaactg aggcaatcca agcttatgac cgtgttcagg
    18421 ctgactatgc tggggcgcgt gcgaccacaa gctggagtca gcgcctagca gaacagtttg
    18481 gtcaagctga accaagctca actagaaaaa atcttgaaca gaagaaatta ttgtagaaag
    18541 tgagaaatta tggccctacc aactattgcc attgtaggac gtcccaatgt tgggaaatca
    18601 accctattta atcggatcgc tggtgagcga atctccattg tagaagatgt cgaaggagtg
    18661 acacgtgacc gtatttatgc aacgggtgag tggctcaatc gttcttttag catgattgat
    18721 acaggaggaa ttgatgatgt cgatgctcct ttcatggaac aaatcaagca ccaggcagaa
    18781 attgccatgg aagaagcaga tgttatcgtt tttgtcgtgt ctggtaagga aggaattact
    18841 gatgcagacg aatacgtagc tcgtaagctt tataagaccc acaaaccagt tatcctcgca
    18901 gtcaacaagg tggacaaccc tgagatgaga aatgatatct atgatttcta tgctctcggt
    18961 ttgggtgaac cattgcctat ctcatctgtc catggaatcg gtacagggga tgtgctagat
    19021 gcgatcgtag aaaatcttcc aaatgaatat gaggaagaaa atccagatgt cattaagttt
    19081 agcttgattg gtcgtcctaa cgttggaaaa tcaagcttga tcaatgctat cttgggagaa
    19141 gaccgtgtta ttgctagtcc tgttgctgga acaactcgtg atgccattga tacccacttt
    19201 acagatacag atggtcaaga gtttaccatg attgatacgg ctggtatgcg taagtctggt
    19261 aaggtttatg aaaatactga gaaatactct gttatgcgtg ccatgcgtgc tattgaccgt
    19321 tcagatgtgg tcttgatggt catcaatgcg gaagaaggca ttcgtgagta cgacaagcgt
    19381 atcgcaggat ttgcccatga agctggtaaa gggatgatta tcgtggtcaa caagtgggat
    19441 acgcttgaaa aagataacca cactatgaaa aactgggaag aagatatccg tgagcagttc
    19501 caatacctgc cttacgcacc gattatcttt gtatcagctt taaccaagca acgtctccac
    19561 aaacttcctg agatgattaa gcaaatcagc gaaagtcaaa atacacgtat tccatcagct
    19621 gtcttgaacg atgtcatcat ggatgccatt gccatcaacc caacaccgac agacaaagga
    19681 aaacgtctca agattttcta tgcgacccaa gtggcaacca aaccaccaac ctttatcatc
    19741 tttgtcaatg aagaagaact catgcacttt tcttacctgc gtttcttgga aaatcaaatc
    19801 cgcaaggcct ttgtttttga gggaacaccg attcatctca tcgcaagaaa acgcaaataa
    19861 aaaagtagaa tctggaatga cagttccgga tttttttgat ataataaaat aatagaaaac
    19921 gctatcaaaa gaagggggga tgtcggtgat ggaacatttg tttaaattct tacttttagc
    19981 accgtacttt tattttgata actggattga aaaggccaac agaaatagta agttttttcc
    20041 aattttttat tatttttact ggttttacat ccccttctat tctcttttta gccttgcttg
    20101 gacagttgtt tcagttctgt ttttcaatac cgtcttgaga aatgtgacag atatcaagtt
    20161 atggggcatt tggtttcttt ttattctgct agctattggt atgaattggt taacttattc
    20221 ctgtttcaaa gaaatgtttc gcttgagaca ggaactaggg aagtctaagg gtggaaggca
    20281 ttgatttata tcggtttcta tttgtaggag gtggcttatg gtacctctaa aatcatttat
    20341 tacacgttat tccaaggttt atattggtaa taacatttaa tagaacgaaa ctaaagaaaa
    20401 caatttaaaa gaggaggctt gtgatggaac acttgtttaa aatggtaatc ttattaccct
    20461 gtttttactt tttcagctgg attgaaaagg caaacagaga tagtaagatt ttcccaatcc
    20521 tctactattt ttactggttt tacatccccc tctattctct ttttagcctt gcttggacag
    20581 ttttttcagt tctgtttttc aatatcgtct tgagaaattt gacagatatc aagttatggg
    20641 gtatttggct attattacta cttatcgctt ttgctagtga ctggttagac tatatctgtt
    20701 tcaaaaaaat gcttgacttg agacgggaac tagggaagtc taaaggcgga aggcattgat
    20761 ttatactctt cgaaaatctc ttcaaaccac gtcaacgtcg ccttggatta tatatgtaac
    20821 tgacttcgtc agtcttatct acaacctcaa agcagtgctt tgagcaactt gcggctagtt
    20881 tcctagtttg ctctttgatt ttcattgagt attatattac tttctatttg taggaggtgg
    20941 cttatgaaga ttcctctctt aactttggca aggcataaat ttgtttatgt cttgcttact
    21001 ttgctttttc ttgctttggt ttatcgtgat gttttgatga cttatttctt ttttgatatt
    21061 catgcgcccg atctagctaa attcgatgga caagcaatta aaaatgactt attaaaatca
    21121 gcattagatt ttcgtattct ccagttcaat ctaggttttt atcaatcatt tattattcca
    21181 atcatcattg ttttgctagg ttttcaatat attgagctga aaaataaagt tttacgattg
    21241 agtattggaa gagaagtgag ctatcaaggg ttaaaaagaa agttgacttt gcaagttgca
    21301 agtatccctt gtttgatata tttagtgact gtgctgataa ttgcaattat aacctatttc
    21361 tttgggactt tttctcctct tgaatggaat tctctatttt ctgatgggag tggtttacaa
    21421 agactcctag atggagagat aaaaagctat ttgttcttta cttgtgtcct actaatcggt
    21481 attttcatca atgcaatcta ttttttacaa atagttgatt atgtggggaa tgtgactcgt
    21541 tcggcaatca cctatttgat gtttctttgg cttggttcta tgctgctcta tagtgccttg
    21601 ccttactata tggttcctat gacgagtttg atgcaagcta gctatgggga tgtaagtttg
    21661 atgaaactct ttactcctta tatcctttat gttgtccctt atatggtact taaaaaatat
    21721 gaagataatg tttaagaatt ttaacaatat ttggctaaag agaaagatcg ttttactact
    21781 tcgtatagtt ctgatgatga ttttgataaa ctatctattg tcaacagcgg ttcaaaagca
    21841 ggatgctgtt atctttttca agagagaatt gatttcaatt ttttcctata atgactattc
    21901 tgaagcgaat ttagaaatcc ccaaactctt gttaaacctt tcgcttttca tggtaggatg
    21961 gctctctgtc attttacttg aaagtgattt ggcagaccat taccatcact tgattcgcta
    22021 tcaatcaagc tcctttttcg attatacaag gaaacgattg gttgtcattt ctaaattttt
    22081 tactcaagat ttgtttgtct ggtttcttgg tttacttccg ctaggaattc atttcaaaac
    22141 agtcgcactt ttctttttac ttgctcagtt aatgatgttg tacttactac tgtcttatct
    22201 gatagcactg attagtgcgg gcgctggttt ttcttttttt ctctattttt tggcatttgt
    22261 gggacaagaa tggatgatgg atcatattgt aacagtgtat ttagtactct taagcttatt
    22321 agttatgttg attgttagtc gcttggaaga gaaatttaag aaaggataaa cgatgagact
    22381 tgaaattata aatggacaga aaatttatgg gaaaagacct attttaaatc agttgaattt
    22441 ggtgtttcaa tcaggaaaaa tttatggact taaaggtgat aatggatctg gcaagacggt
    22501 tcttttaaag atacttgctg gttatattaa gcttgacaaa ggaaaagttc ttcaagatgg
    22561 taaagtttac ggggtaaaaa atcattatat tcaggatgca ggaattttaa ttgaaaaagt
    22621 cgagttttta tctcatttat ccctgagaga aaatttggaa ctgttaaggt atttttcatc
    22681 taaagttacg gaaaaaagaa ttgcctattg gattcaatac tatgatttac aggaatttga
    22741 agacgttgaa taccgtcatt tatccttggg aacaaagcaa aaaatggcct tgattcaagc
    22801 ctttatttcc tctccttcta tactctttct cgatgaacct atgaatgctt tggatgagaa
    22861 gagtgtgagg ttaaccaaac aggtcatttt atcttacctg aaaaaagaaa atggtctggt
    22921 tatcctgacg tcgcacatat cggaagatat ttcagacctt tgtacagatg tattagttgt
    22981 cgaaaatgga catatacaat atgtaaagga tatacaatcc taggagatgg cttatggcac
    23041 atctaaaatc atttattaca cgctattcca aggtttatat tggtttagtt ctgctgatct
    23101 ggctgtcttt cttctttatc ccttgggata gagcgattct gggggtaagg attgacatct
    23161 tcatcatgca gaaaatcttg ctagcttttg gaattctgtc cattctcatg gccttgctgt
    23221 ccaagaaagt cagtctcttt gtttttggac tgatttgctg tctttctctt tggattaact
    23281 tatttatcac atttgccatt ttgccgattt ttggcaatta aacagtcata aaagtcggag
    23341 aggttagctt gaaaactaac ctctttttcc ttttcaaaat ggggattctt ccttgaaaat
    23401 aatcagtaat tgtgctaaaa ttaaaggaac attctaaaat attcggaatt taaagtaagg
    23461 aaaaacatgg ctaatatttt aaaaacaatt atcgaaaatg ataaaggaga aatccgtcgt
    23521 ctggaaaaga tggctgacaa ggttttcaaa tacgaagacc aaatggctac tttgactgac
    23581 gaccaactaa aagcaaaaac agttgaattt aaggaacgtt atcaaaatgg agaatcactg
    23641 gattcattgc tttacgaagc atttgcggtt gtccgtgaag gtgccaaacg tgtcctaggt
    23701 ctcttccctt ataaggttca ggtcatgggg gggattgttc ttcaccatgg tgacgtgcca
    23761 gagatgcgta caggggaagg gaaaaccttg actgcgacca tgccggtata cctcaatgcc
    23821 ctttcaggta aaggggttca cgtagttacg gttaatgaat acctgtcaga acgtgacgcg
    23881 actgagatgg gtgaattgta ctcttggctt ggtttgtcag tagggattaa cttggctacc
    23941 aaatctccaa tggagaaaaa agaagcctat gagtgtgata ttacttactc aactaactca
    24001 gaaatcggat ttgactacct tcgtgacaac atggtcgttc gcgccgaaaa catggtacaa
    24061 cgtccgctta actatgcctt ggtcgatgag gttgactcta tcttgattga cgaggctcgt
    24121 acacctttga ttgtatcagg tgccaatgcg gttgaaacca gtcagttgta tcacatggca
    24181 gaccactatg taaaatcttt gaacaaagat gactacatca tcgatgtgca gtctaagact
    24241 attggtttgt ctgattcagg gattgacagg gctgaaagct acttcaaact tgaaaacctc
    24301 tatgacatcg aaaacgtggc tttgactcac tttatcgata acgcccttcg tgccaactac
    24361 atcatgcttc tcgatattga ctatgtggtg agcgaagagc aagaaatctt gattgtcgac
    24421 caatttacag gtcgtaccat ggaaggtcgt cgttattctg atggattgca ccaagctatt
    24481 gaagccaaag aaggtgtgcc aatccaggat gaaaccaaga catctgcctc aatcacgtac
    24541 caaaaccttt tccgtatgta caagaaattg tctggtatga cgggtacagg taagactgag
    24601 gaagaagaat ttcgtgaaat ctacaacatt cgtgttattc caatcccaac aaaccgtcct
    24661 gttcaacgta ttgaccactc agaccttctt tatgcaagta tcgaatctaa gtttaaagcg
    24721 gttgtcgaag acgttaaggc tcgttaccaa aagggtcaac ctgtcttggt tggtacagta
    24781 gcggttgaaa ctagtgacta catttctaag aaattggttg cagctggtgt tcctcacgaa
    24841 gtcttgaatg ccaaaaacca ctatagagaa gcccaaatca tcatgaatgc tggtcaacgt
    24901 ggtgccgtta ccatcgcaac caacatggcg ggtcgtggta ccgacatcaa gcttggtgaa
    24961 ggtgttcgtg aacttggagg actttgtgtt attggtacag aacgtcatga aagtcgtcgt
    25021 atcgataacc agcttcgtgg acgttcaggt cgtcaaggag atccaggtga gtcacaattc
    25081 tacctatctc ttgaagatga tttgatgaaa cgttttggtt ctgaacgctt gaagggaatc
    25141 tttgaacgct tgaacatgtc tgaagaggcc attgagtctc gcatgttgac gcgtcaggtt
    25201 gaagcagctc agaaacgtgt cgaaggaaat aactacgata cccgtaaaca agtccttcaa
    25261 tacgatgatg tcatgcgtga acaacgtgag attatctatg ctcaacgtta cgatgtcatc
    25321 actgcagatc gtgacttggc acctgaaatt cagtctatga tcaaacgcac gattgaacgt
    25381 gtcgttgatg gtcatgcgcg tgccaaacaa gatgaaaaac tagaggcaat tttgaacttt
    25441 gctaagtaca acttgcttcc tgaagattct attacgatgg aagacttgtc aggcttgtct
    25501 gataaggcca tcaaggaaga gcttttccaa cgtgccttga aggtttacga tagtcaggtt
    25561 tcaaaactac gcgatgaaga agcagttaaa gaattccaaa aagttttgat tctacgagtg
    25621 gtggataaca agtggacaga tcatatcgat gcccttgatc aattgcgtaa cgcggttgga
    25681 cttcgtggct atgctcagaa caaccctgtt gttgagtatc aggcagaagg tttccgtatg
    25741 tttaatgata tgattggttc gattgagttt gatgtgacac gcttgatgat gaaagcacaa
    25801 attcatgaac aagaaagacc acaggcagaa cgtcatatca gtacaacagc gactcgcaat
    25861 atcgctgctc accaagcaag tatgccagaa gatttggatt tgagccagat tggacgcaat
    25921 gaactttgcc catgtggttc tggtaagaaa tttaaaaact gtcacggtaa aagacaataa
    25981 aatgagatag tttagaggcg gatatcttgt gaaaagtaaa tttttactgg gtatccgttt
    26041 gctttataag gagatgagtt atggtattta cagcaaaaag ctctaaaata aatatagaag
    26101 aagttcgtgc cttgtcaaaa ttagaaggtc aggctttgga gaggaaatca cagcgagatc
    26161 aagagctaga agccattata cgtggagaag accagcgaat tctcttggta atcgggccat
    26221 gctcatctga caacgaagaa gctgtccttg aatacgctaa gcgtttggca gtcctacaag
    26281 aagaagtggc agatcgtatc tttatggtta tgcgtgttta tactgccaaa ccccgtacca
    26341 acggagatgg ctataagggc ttgattcacc agcctaacgc gacagaagcg cctagtctta
    26401 tcaatggaat caaagccgtt cgccatcttc actatcgtgt catcacagaa acagggatga
    26461 caactgctga tgaaatgctt tatcctgaaa accttccgct tgtagatgat ttgatttctt
    26521 acatggcagt tggtgcccgt tcagttgaag accagcaaca ccgctttgtg gcaagtgggg
    26581 caggattttc tactggtttt aaaaatccaa cctctggaaa tctcaatgtc atgtttaatg
    26641 ggatttatgc tgctcaaaac aaacaaagtt tccttttctt aggaaaagaa gtagaaacaa
    26701 ctgggaaccc gctttcacac gctattcttc gtggtgctct taatgagtat ggaaaaaata
    26761 ttcccaacta ctattatgac aatttaattg ataccattgc ccagtatgag aaaatgggct
    26821 tggaaaatcc ttttatcatc attgatacca atcatgacaa ttctggtaag cagtatattg
    26881 aacagatccg aattgtccgc cagaccttga ttaaccgtgc ttggaatgaa aaaattaagc
    26941 agttcgttcg tggttttatg attgagtctt atctggaaga tggtcgacaa aatgagccag
    27001 aagtatttgg taagtctatc acagaccctt gcctgggttg ggataacaca gaagctcttg
    27061 tcagagaaat ttacaaaacg ttaggagaat aagatggcat ttattgaaaa aggtcaagaa
    27121 atcgatatgg aagtcatcaa ggctgaaacc caattgtctg cggaagcctt gagactcaag
    27181 gaaagccgtg acagggaatt ggcagatatt atttcagggg aagatgaccg tattctcttg
    27241 gtgattggtc cttgctcttc tgataatgaa gaggcggtct tggaatatgc tcgccgttta
    27301 tccgccttgc aaaagaaggt agcggacaag attttcatgg tcatgcgcgt gtatactgct
    27361 aagcctcgta ccaatggaga cggctataaa ggattggttc accagccaga tacttctaag
    27421 gctccaagcc tgattaacgg cttgcaggct gtgcgccagt tgcactaccg cgtgattaca
    27481 gagactggtt tgacaacggc agatgagatg ctttatccgt caaatctgat cttggtggat
    27541 gacttggtca gctaccatgc cgttggagct cgttctgtgg aagaccaaga gcaccgcttt
    27601 gtggcttctg ggattgatgc accagtaggg atgaaaaatc caacctcagg aaatttgggt
    27661 gttatgttta acgccatcta tgctgctcaa aacaagcaaa ccttccttta tcatgggcag
    27721 gaagttgaga catcaggtaa tcctttggcc catgttatcc tccgtggagc agtcaacgag
    27781 tatggcaatt atatgccgaa ttactactat gaaaacctac tccaagccat tgaacgctat
    27841 gaaaccatgg gacttgaaaa tccttttatc ctcattgaca ccaaccatga taactcaggc
    27901 aagcaatata tggagcagat tcgaattgtt cgccagacct tgcagaatcg tgattggaat
    27961 gagaaaatta aaaagacggt tcgaggattt atgattgaat cttacctagc agatggtcgt
    28021 caaaaccaac cagagatctt tggttgctct attactgacc cttgcctagg ttgggaaaat
    28081 acagaggcct tggtagaaga gatttatgtt accttgacaa aataagtgaa aaggatggag
    28141 ttggggaatc tcaactcctt ttgatgagaa tgatagttgg acacggaatt gacatcgaag
    28201 aattggcttc gatagaaagc gcagttacac gacatgaagg atttgctaag cgtgtactga
    28261 ccgctcagga aatggagcgc ttcaccagtc tcaaaggacg caggcaaata gaatatttag
    28321 ctggtcgctg gtcggctaag gaggcctttt ccaaggctat gggaacgggc attagcaagc
    28381 tcggttttca ggatttggaa gtcttgaaca atgaacgtgg ggcgccttat tttagtcagg
    28441 caccattttc aggaaagatt tggctgtcta tcagccacac cgatcagttt gtgacagcca
    28501 gtgtcatttt ggaggaaaat catgaaagct agtccacata gaccaaccaa ggctctgatt
    28561 catctgggag ctattcgaca aaatattcag caaatggggg ctcatatccc tcaaggaacg
    28621 ctcaagttgg ctgtggtcaa ggccaatgct tatggtcatg gagctgttgc cgttgccaag
    28681 gcaattcaag atgatgttga tggcttttgc gtttccaata tcgatgaagc cattgaactc
    28741 agacaagctg gactcagcaa gccaatcctc attttaggag tttctgaaat cgaagctgtt
    28801 gctctagcta aagaatatga cttcaccttg acagtggctg gactggagtg gattcaagca
    28861 ctcttagata aggaagtgga cctaactgga ttgacagtcc acctcaagat tgattcaggg
    28921 atgggacgga ttggttttag agaggcaagt gaggttgagc aggctcaaga tttgctccaa
    28981 caacacggtg tttgtgttga aggaatcttt acccactttg ctactgctga tgaggaatca
    29041 gatgactatt ttaatgccca gttagaacgg tttaaaacta ttttagctag tatgaaggaa
    29101 gctccagagc tggttcatgc tagcaattct gcaacgactc tttggcatgt agagactatt
    29161 ttcaatgcgg ttcgtatggg agatgccatg tatggtctca atccaagtgg agcggtcttg
    29221 gatttgcctt atgatttgat accggccttg accttggagt ctgctctggt tcatgtcaag
    29281 acagttccag ctggagcttg catgggctat ggagcaactt atcaagcgga tagcgagcaa
    29341 gtcatcgcaa ccgtgccaat cgggtatgca gatggatgga caagagacat gcaaaatttc
    29401 tctgtcttgg tagatggcca agcttgccca attgtcggca gggtttcgat ggaccaaatc
    29461 actattcgat tgcctaagcc ttacccactc ggaaccaagg taaccttgat tggctccaat
    29521 ggggataagg aaatcactgc aactcaggta gcgacctacc gcgtaaccat taactatgag
    29581 gtggtttgcc ttctcagcga ccgtattccg agagaatatt attagaaaag aaaggagtgg
    29641 agcatgaatc tacatcaacc cttgcatgtc ttgcctggtg tgggaccaaa gtcagcagaa
    29701 aaatacgcca aactaggaat tgaaaacttg caagatctct tgctctactt tcctttccgt
    29761 tatgaagact tcaaaaccaa gcaggtgctg gagctggaag acggtgagaa ggcagttctt
    29821 tctggtcagg tagtgactcc tgctagtgtc cagtattatg gtttcaagcg caatcgcctg
    29881 cgttttagtc tcaagcaggg agagatcgtt tttgcggtga atttctttaa ccagccctat
    29941 ctggctgata aaatagagtt gggagcaacc cttgctgtct ttggaaaatg ggaccgcgct
    30001 aaggctagtc tgactgggat gaaggttctg gctcaggtag aagatgacct ccagcctgtc
    30061 tatcgtctgg ctcagggaat cagtcaggcc agtctggtta aggtcatcaa gacggctttt
    30121 gatcagggac tagacctctt gatagaagaa aatctgcccc agtctttact agacaaatac
    30181 aaactcatgt cccgttgtca ggcagtccga gctatgcatt ttccaaagga tttggcagaa
    30241 tacaagcagg ctcttcgccg tataaagttt gaggaactct tttatttcca aatgcagctg
    30301 cagatgctca agtctgaaaa tagagttcag ggaagtggtc tggttctgaa ttggtctcag
    30361 gaaaaagtga cagcagttaa agtaagtctt ccttttgccc tgacccaagc tcaggaaaag
    30421 agtttgcagg aaattttaac tgatatgaag tccgaccacc acatgaatcg tctcctacaa
    30481 ggggatgtgg ggagtggaaa aacggtagtc gctggcttgg ccatgtttgc ggcagtgaca
    30541 gcaggttatc aggctgccct aatggtacca acagaaatcc tcgcagagca acactttgag
    30601 agtttacaga acctttttcc caatttgaaa ctggctctct tgacaggttc cttgaaagct
    30661 gcagaaaaga gagaggtctt ggagaccatt gccaagggtg aggctgattt gattatcgga
    30721 actcacgctc tgatacaaga tggggtggag tatgctcgtc ttggtttgat tattatcgat
    30781 gagcagcacc gttttggtgt agggcaaagg cgtattttac gggaaaaagg tgacaatcca
    30841 gatgtcctca tgatgacggc gactcccatt ccacggacgc ttgccatcac agcctttgga
    30901 gatatggatg tttccattat cgaccagatg ccagcaggtc ggaagcctat tgtgacgcgc
    30961 tggatcaaac atgagcaact acctcaggtc ttgacttggt tagaggggga aattcaaaaa
    31021 ggttcccaag cctatgtcat ctctcctttg attgaagaat cagaagctct agatttgaaa
    31081 aatgccattg ccttatcaga ggagttgacg actcattttg caggcaaggc agaggtggct
    31141 cttctacatg gtaggatgaa gagtgacgaa aaagaccaga tcatgcagga tttcaaggag
    31201 agaaagacgg atattctggt ttcgacgacg gttattgagg ttggggtcaa cgttcccaat
    31261 gcgactgtca tgattatcat ggatgccgat cgcttcggtc tcagtcaact tcaccagctt
    31321 agaggtcgtg tcggtcgggg ggacaagcag tcctacgctg ttctcgttgc caatcccaag
    31381 acggattctg ggaaagaccg catgcgcatc atgacagaaa cgaccaatgg atttgtcctt
    31441 gcggaggaag atttgaaaat gcgtggttct ggtgagattt ttggaaccag acagtcagga
    31501 cttccagagt tccaagtggc tgatattatc gaagattttc cgattttaga agaagcaaga
    31561 aaggttgcta gctacattag ttctatagaa gcttggcaag aagatccaga gtggcgcatg
    31621 attgcccttc atctggaaaa gaaagaacat ctggattaag ctttctctaa ggaaaactta
    31681 agctagtttt taggtttggc tcttatacta aagtcatcaa aaagaaacga ggactctcat
    31741 atgacagtaa cgattaaagt aaattaccaa accactttcc aaaagaagga agcaaaaaac
    31801 tagtataaac agaagagaga gcgaaatgct ctttttcgtt tctaaaacta ctttcagccc
    31861 atcatcctaa aagtaaagaa tctaaattca ctttctattt atccttcttt cttgcattga
    31921 ttacatagat atgctacagt tgtggtaacg attacaaaat aaaaggagca tgctatgaaa
    31981 aatccagctt tgctagaaga aattaagacc tatagaggaa gggatgaggt tccggaagac
    32041 tttgatgctt tctgggatgg ggaagtgaaa aatgtttcca cgcttccatc ctaccacttg
    32101 gaggaaagag atttccacat tcctcaagtc aagtgctatg agttaacatt tgaaggaagc
    32161 aaggaaggaa aggtctatgc acgcattgtt cttccaaaga gtgaggagaa ggttccacta
    32221 atcttccatt ttcatggtta tatgggacgt ggctgggact gggccgacat gctgggcttc
    32281 accgtagctg gttacggtgt tgtttccatg gatgtgcggg gccagtcagg ttactcacaa
    32341 gacggcttgc gttctccttt aggaaatacc gtgaaggggc atattatccg tggtgctatg
    32401 gaaggtcggg accacctctt ttataaggat gtttatctgg atatttacca gttggtcgaa
    32461 attgttgcta gtctgtctca ggttgatgag aagcgtcttt ctagctatgg tgcctcacaa
    32521 ggaggggctc tagctctagt tgcagcagcg ctcaatcctc gaattcagaa aacagttgcc
    32581 atttatccct tcttgtcaga cttcagacgg gtgattgaga ttggtaatac tagcgaggct
    32641 tacgacgaac ttttccgtta tttcaagttt cacgacccct tccatgaaac agaggaggaa
    32701 atcatggcga cccttgccta tatcgatgtc aaaaatcttg cccatcgtat ccaaggtgag
    32761 gttaagatga ttacgggctt ggacgacgat gtttgctatc ccattaccca gtttgcgatt
    32821 tataatcgtc tgacctgcga taaaacctat cgcatcatgc ctgagtatgc tcacgaagcc
    32881 atgaatgtat ttgtcaatga ccaagtctac aactggctct gtggaagtga gattcctttt
    32941 aaatatctaa aataaggagt cgactctaag cacaaaatct taaaaattac aaacacgcat
    33001 agtatcaggg gattaaaaaa acttgatact atgcgtttta tcatggaaat atagtaaaat
    33061 gaaacaagaa taggacaaaa cgatcaggac agtcaaatcg atttctaaca atgttttaga
    33121 agcaaatgtg tactattcta gtttcaatct attatattta tagaattttt tgttgctaga
    33181 tttgtcaaat tgcttaaaat aatttttttc agaaagcaaa agccgatacc tatcgagtag
    33241 ggtagttctt gctatcgtca ggcttgtctg taggtgttaa tacttttcaa aaatctcttt
    33301 aaaccacgtc agcttcgcct tgccgtatat atgttactga cttcgtcagt tctatctgcc
    33361 acctcaaaac ggtgttttga gctgacttcg tcagttctat ctacaacctc aaaacagtgt
    33421 tttgagcatc atgcggctag cttcttagtt tgctctttga ttttcattga gtataaaaac
    33481 agatgagttt ctgttttctt tttatggact ataaatgttc agctgaaact actttcaagg
    33541 acattattat ataaaagaat tttttgaaac taaaatctac tatattacac tatattgaaa
    33601 gcgttttaaa aatgaggtat aataaattta ctaacgctta taaaaagtga tagaatctat
    33661 ttttatgtat atttaaagat agattgctgt aaaaatagta gtagctatgc gaaataacag
    33721 atagagagaa gggattgaag cttagaaaag gggaataata tgatatttaa ggcattcaag
    33781 acaaaaaagc agagaaaaag acaagttgaa ctacttttga cagttttttt cgacagtttt
    33841 ctgattgatt tatttcttca cttatttggg attgtcccct ttaagctgga taagattctg
    33901 attgtgagct tgattatatt tcccattatt tctacaagta tttatgctta tgaaaagcta
    33961 tttgaaaaag tgttcgataa ggattgagca ggaagtatgg tgtaaatagc ataggctgat
    34021 gtccatcatt tgcttataaa gagatatttt agtttaattg cagcggtgtc ctggtagata
    34081 aactagattg gcaggagtct gattggagaa aggagagggg aaaattggca ccaatttgag
    34141 atagtttgtt tagttcattt ttgtcattta aatgaactgt agtaaaagaa agttaataaa
    34201 agacaaacta agtgcatttt ctggagtaaa tgtcttattt cagaaatcgg gatatagata
    34261 tagagaggat cagtatgaat cggagtgttc aagaacgtaa gtgtcgttat agcattagga
    34321 aactatcggt aggagcggtt tctatgattg taggagcagt ggtatttgga acgtctcctg
    34381 ttttagctca agaaggggca agtgagcaac ctctggcaaa tgaaactcaa ctttcggggg
    34441 agagctcaac cctaactgat acagaaaaga gccagccttc ttcagagact gaactttctg
    34501 gcaataatca agaacaagaa aggaaagata agcaagaaga aaaaattcca agagattact
    34561 atgcacgaga tttggaaaat gtcgaaacag tgatagaaaa agaagatgtt gaaaccaatg
    34621 cttcaaatgg tcagagagtt gatttatcaa gtgaactaga taaactaaag aaacttgaaa
    34681 acgcaacagt tcacatggag tttaagccag atgccaaggc tccagcattc tataatctct
    34741 tttctgtgtc aagtgctact aaaaaagatg agtacttcac tatggcagtt tacaataata
    34801 ctgctactct agaggggcgt ggttcggatg ggaaacagtt ttacggtaat tacaacgatg
    34861 cacccttaaa agttaaacca ggtcagtgga attctgtgac tttcacagtt gaaaaaccga
    34921 caccagaact acctaaaggc cgagtgcgcc tctacgtaaa cggggtatta tctcgaacaa
    34981 gtctgaaatc tggcaatttc attaaagata tgccagatgt aacgcatgta caaatcggag
    35041 caaccaagcg tgccaacaat acggtttggg ggtcaaatct acagattcgg aatctcactg
    35101 tgtataatcg tgctttaaca ccagaagagg tacaaaaacg tagtcaactt tttaaacgct
    35161 cagatttaga aaaaaaacta cctgaaggag cggctttaac agagaaaacg gacatattcg
    35221 aaagcgggcg taacggtaaa ccaaataaag atggaatcaa gagttatcgt attccagcac
    35281 ttctcaagac agataaagga actttgatcg caggtgcaga tgaacgccgt ctccattcga
    35341 gtgactgggg tgatatcggt atggtcatca gacgtagtga agataatggt aaaacttggg
    35401 gtgaccgagt aaccattacc aacttacgtg acaatccaaa agcttctgac ccatcgatcg
    35461 gttcaccagt gaatatcgat atggtgttgg ttcaagatcc tgaaaccaaa cgaatctttt
    35521 ctatctatga catgttccca gaagggaagg gaatctttgg aatgtcttca caaaaagaag
    35581 aagcctacaa aaaaatctat ggaaaaacct atcaaatcct ctatcgtgaa ggagaaaagg
    35641 gagcttatac cattcgagaa aatggtactg tctatacacc agatggtaag gcgacagact
    35701 atcgcgttgt tgtagatcct gttaaaccag cctatagcga caagggtgat ctatacaagg
    35761 gtaaccaatt actaggcaat atctacttca caacaaacaa aacttctcca tttagaattg
    35821 ccaaggatag ctatctatgg atgtcctaca gtgatgacga cgggaagaca tggtcagcgc
    35881 ctcaagatat tactccgatg gtcaaagccg attggatgaa attcttgggt gtaggtcctg
    35941 gaacaggaat tgtacttcgg aatgggcctc acaagggacg gattttgata ccggtttata
    36001 cgactaataa tgtatctcac ttaaatggct cgcaatcttc tcgtgtcatc tattcagatg
    36061 atcatggaaa aacttggcat gctggagaag cggtcaacga taaccgccag gtagacggtc
    36121 aaaagatcca ctcttctacg atgaacaata gacgtgcgca aaatacagaa tcaacggtgg
    36181 tacaactaaa caatggagat gttaaactct ttatgcgtgg tttgactgga gatcttcagg
    36241 ttgctacaag taaagacgga ggagtgactt gggagaagga tatcaaacgt tatccacagg
    36301 ttaaagatgt ctatgttcaa atgtctgcta tccatacgat gcacgaagga aaagaataca
    36361 tcatcctcag taatgcaggt ggaccgaatc gtgaaaatgg gatggtccac ttggcacgtg
    36421 tcgaagaaaa tggtgagttg acttggctca aacacaatcc aattcaaaaa ggagagtttg
    36481 cctataattc gctccaagaa ttaggaaatg gggagtatgg catcttgtat gaacatactg
    36541 aaaaaggaca aaatgcctat accctatcat ttagaaaatt taattgggaa tttttgagca
    36601 aagatctgat ttctcctacc gaagcgaaag tgaagcgaac tagagagatg ggcaaaggag
    36661 agatgggcaa aggagttatt ggcttggagt ttgactcaga agtattggtc aacaaggctc
    36721 caacccttca attggcaaat ggtaaaacag cgactttcct aacccagtat gatagcaaga
    36781 ccttgttgtt tgcagtagat aaggaagata tcggacagga aattattggt atagctaaag
    36841 gaagcatcga aagtatgcat aatcttcctg taaatctagc aggtgccaga gttcctggcg
    36901 gagtaaatgg tagcaaagca gcggtgcatg aagttccaga atttacaggg ggagttaatg
    36961 gtacagagcc agctgttcat gaaatcgcag agtataaggg atctgattcg cttgtaactc
    37021 ttactacaaa agaagattat acttacaaag ctcctcttgc tcagcaggca cttcctgaaa
    37081 caggaaacaa ggagagtgac ctcctagctt cactaggact aacagctttc ttccttggtc
    37141 tgtttacgct agggaaaaag agagaacaat aagagaagaa ttctaaacat ttgattttgt
    37201 aaaaatagaa ggagatagca ggttttcaag cctgctatct ttttttgatg acattcaggc
    37261 tgatacgaaa tcataagagg tctgaaacta ctttcagagt agtctgttct ataaaatata
    37321 gtagattgaa ataagatgtg aacaactcta tcaggaaagt caaattaatt tatagaaaaa
    37381 ttttagcagt caaggtgtac tgttatagat tcaatatatt atgcgactat taaccttctc
    37441 ttctcctaaa attgactttc ttgttttctt atcttgtcca ctcgaaacaa gtattgtaag
    37501 aatttgatta tttttgaaag tacttttaat atacttgata tagttaaaaa agatttgaaa
    37561 ctaaattcca aattagaaaa agacttgaaa t
//
LOCUS       DAVBGY010000015        37214 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVBGY010000015 DAVBGY010000000
VERSION     DAVBGY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37214
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.015"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            175..1938
                     /gene="aspS"
                     /locus_tag="VQ620_000779"
     CDS             175..1938
                     /gene="aspS"
                     /locus_tag="VQ620_000779"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET2763442.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVVNATLGTLRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            1916..2857
                     /locus_tag="VQ620_000780"
     CDS             1916..2857
                     /locus_tag="VQ620_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET2763443.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(2977..3057)
                     /locus_tag="VQ620_000781"
                     /pseudo
     CDS             complement(2977..3057)
                     /locus_tag="VQ620_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(3193..4179)
                     /locus_tag="VQ620_000782"
     CDS             complement(3193..4179)
                     /locus_tag="VQ620_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2763444.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(4189..4989)
                     /locus_tag="VQ620_000783"
     CDS             complement(4189..4989)
                     /locus_tag="VQ620_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HET2763445.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRIYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(5250..6092)
                     /locus_tag="VQ620_000784"
     CDS             complement(5250..6092)
                     /locus_tag="VQ620_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2763446.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(6094..7386)
                     /locus_tag="VQ620_000785"
     CDS             complement(6094..7386)
                     /locus_tag="VQ620_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2763447.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGNKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(7497..8768)
                     /locus_tag="VQ620_000786"
     CDS             complement(7497..8768)
                     /locus_tag="VQ620_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2763448.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            9318..10835
                     /gene="malQ"
                     /locus_tag="VQ620_000787"
     CDS             9318..10835
                     /gene="malQ"
                     /locus_tag="VQ620_000787"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET2763449.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            10861..13119
                     /locus_tag="VQ620_000788"
     CDS             10861..13119
                     /locus_tag="VQ620_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HET2763450.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(14163..14735)
                     /locus_tag="VQ620_000789"
     CDS             complement(14163..14735)
                     /locus_tag="VQ620_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763451.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAINLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(15357..16205)
                     /locus_tag="VQ620_000790"
     CDS             complement(15357..16205)
                     /locus_tag="VQ620_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET2763452.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            16350..16628
                     /locus_tag="VQ620_000791"
     CDS             16350..16628
                     /locus_tag="VQ620_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763453.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            16630..18693
                     /locus_tag="VQ620_000792"
     CDS             16630..18693
                     /locus_tag="VQ620_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET2763454.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(18771..20027)
                     /locus_tag="VQ620_000793"
     CDS             complement(18771..20027)
                     /locus_tag="VQ620_000793"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET2763455.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAIWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            20169..22634
                     /locus_tag="VQ620_000794"
     CDS             20169..22634
                     /locus_tag="VQ620_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET2763456.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            22699..23610
                     /locus_tag="VQ620_000795"
     CDS             22699..23610
                     /locus_tag="VQ620_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET2763457.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCLEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVSVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            23840..24538
                     /gene="dapD"
                     /locus_tag="VQ620_000796"
     CDS             23840..24538
                     /gene="dapD"
                     /locus_tag="VQ620_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HET2763458.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVCVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            24606..25736
                     /locus_tag="VQ620_000797"
     CDS             24606..25736
                     /locus_tag="VQ620_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HET2763459.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFTSQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            25748..26287
                     /locus_tag="VQ620_000798"
     CDS             25748..26287
                     /locus_tag="VQ620_000798"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET2763460.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLTPKTYPKGRMDFVVYDPQQLVKTSFALLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGRALSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            26271..26948
                     /locus_tag="VQ620_000799"
     CDS             26271..26948
                     /locus_tag="VQ620_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET2763461.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(27020..27919)
                     /gene="galU"
                     /locus_tag="VQ620_000800"
     CDS             complement(27020..27919)
                     /gene="galU"
                     /locus_tag="VQ620_000800"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET2763462.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(27941..28957)
                     /locus_tag="VQ620_000801"
     CDS             complement(27941..28957)
                     /locus_tag="VQ620_000801"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET2763463.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     YHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKAI
                     IMHASKGLEPDSHKRLSTILEEEIPEQLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            29169..29547
                     /locus_tag="VQ620_000802"
                     /pseudo
     CDS             29169..29547
                     /locus_tag="VQ620_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(29727..30152)
                     /locus_tag="VQ620_000803"
     CDS             complement(29727..30152)
                     /locus_tag="VQ620_000803"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2763464.1"
                     /translation="MNNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKSLTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(30220..30870)
                     /gene="phoU"
                     /locus_tag="VQ620_000804"
     CDS             complement(30220..30870)
                     /gene="phoU"
                     /locus_tag="VQ620_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET2763465.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(30885..31637)
                     /gene="pstB"
                     /locus_tag="VQ620_000805"
     CDS             complement(30885..31637)
                     /gene="pstB"
                     /locus_tag="VQ620_000805"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2763466.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDVLLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(31639..32454)
                     /gene="pstA"
                     /locus_tag="VQ620_000806"
     CDS             complement(31639..32454)
                     /gene="pstA"
                     /locus_tag="VQ620_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET2763467.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(32447..33310)
                     /gene="pstC"
                     /locus_tag="VQ620_000807"
     CDS             complement(32447..33310)
                     /gene="pstC"
                     /locus_tag="VQ620_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET2763468.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(33428..34303)
                     /locus_tag="VQ620_000808"
     CDS             complement(33428..34303)
                     /locus_tag="VQ620_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HET2763469.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(34470..35801)
                     /locus_tag="VQ620_000809"
     CDS             complement(34470..35801)
                     /locus_tag="VQ620_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HET2763470.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSALKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(35802..36509)
                     /locus_tag="VQ620_000810"
     CDS             complement(35802..36509)
                     /locus_tag="VQ620_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2763471.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(36593..37078)
                     /locus_tag="VQ620_000811"
     CDS             complement(36593..37078)
                     /locus_tag="VQ620_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET2763472.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            37064..37214
                     /locus_tag="VQ620_000812"
                     /pseudo
     CDS             37064..37214
                     /locus_tag="VQ620_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT        10906 a         7058 c         8024 g        11226 t
ORIGIN      
        1 atacctacgg taaggcgacg ctgacgtggt ttgaagagat tttcgaagag tattagcgct
       61 aatttttagg gctttcacct gacttttaaa gactggcaaa cttttctgat gattttcatg
      121 aagagcctgc gcttttatgg taaaatagta acagaataaa agaggagaga aataatgaaa
      181 cgtagtatgt atgctggtcg tgttcgtgag gaacacatcg gacaagaaat aaccttgaaa
      241 ggatgggttg gccgtcgtcg tgaccttggt ggtttgatct ttatcgatct tcgtgaccgt
      301 gaaggaatca tgcagttggt tatcaaccct gaaaaagtat ctgcagaggt tatggcaaca
      361 gctgaaagcc ttcgtagcga atttgttatt gaggtgactg gtcaggtcgc tgcgcgtgag
      421 caagccaatg ataagttgcc aactggtgcg gttgagttaa acgtgacagc tcttattgtg
      481 cttaacacag ctaagacaac accatttgag attaaggatg gcattgaggc aaatgacgat
      541 acacgtttgc gttaccgtta ccttgacctt cgtcgtccag aaatgttgga aaatcttaaa
      601 cttcgtgcca aggtgaccca ctctatccgc aactatttgg atgagttgga gtttatcgac
      661 gtggagacac cattcctttc taagtcaacg cctgaagggg cgcgtgatta tttagtgccg
      721 tctcgtgtta ataaggggca tttttacgct cttcctcaaa gtccacaaat cacgaaacag
      781 ctcttgatga atgctggttt tgaccgttac taccaaatcg ttaaatgttt ccgtgacgag
      841 gacttgcgtg gagaccgcca gcctgaattt actcaggtcg acttggaaac gtccttcctt
      901 actgagaaag aaatccaaga tattacagaa ggcttgatcg cgcgcgtgat gaaggaaaca
      961 aaaggcatcg aagtaacgct accgttccct cgtatgaaat acgatgatgc tatggctctt
     1021 tacggttctg acaagccaga tactcgtttt gacatgttgc ttcaggactt gacagaagtg
     1081 gtcaaaggtg tagactttaa agtcttttca gaagcacctg ctgtaaaagc gattgtggtc
     1141 aaaggagctg cggacaacta ttcacgtaaa gacatcgaca agatgacgga agtagccaaa
     1201 cagtatggtg ccaaaggtct tgcttgggtc aaggtggttg atggagaatt aaacggacca
     1261 gttgccaagt tcttgactgg tatccaagaa gaattgacaa cagcgcttgc tcttgaagat
     1321 aaggacttgg ttctctttgt ggcggatacg cttgaagtgg ttaatgcaac actgggtacc
     1381 cttcgtggac gtattgctaa agagcttggc ttgattgata atgataagtt caacttcctt
     1441 tgggtggttg actggccgat gtttgaatgg tctgaagaag aaggccgcta catgagcgcc
     1501 caccatccat tcacacttcc acaggaagag actgctcacg aattagaagg tgatttggct
     1561 aaggttcgtg ccattgctta cgatatcgtc ttgaacggtt atgagcttgg tggtggtagc
     1621 cttcgtatca accaaaaaga ccttcaagaa cgcatgttca aggctcttgg tttctcaact
     1681 gaagaagcca atgaccagtt tggtttcctt cttgaagcca tggactatgg tttcccaccg
     1741 cacggtggtt tggctatcgg gcttgaccgt tttgtcatgt tgcttgctgg agaagaaaat
     1801 atccgtgaag tcattgcctt tcctaagaac aacaaggcaa ctgacccaat gacacaagct
     1861 ccatcaacag tcgctctcaa acaactagag gaactcagct tacaagtaga agaagatgaa
     1921 acaaacaaaa cgaattaagc ggtggcgcta ttatctgcgc cgctttgctt atcagataaa
     1981 aattttacgt gtcttacaaa gtatctctcg agaaaagtat gatgagaaga tttcggcctc
     2041 tctggtctat ggttttttat cagcagtagc agttaatttc tttttccaac cagggcatgt
     2101 gtattcgagt ggtgcaacag gtctggcaca gattatctct gccttgagta atcactggtt
     2161 tggttttcat attccgattt cgctaagctt ctacgccatt aacttccctt tgatggtctt
     2221 agcttggtat cagattggcc ataagttcac cgtctttacc tttatcacgg tatctatgag
     2281 ttccttcttt atccagtttg tccctgtggc aaccttgacg gaggatccca ttatcaattc
     2341 cctttttggg ggtgttgtta tgggtttggg gattggtttt gctcttcgaa acaatatctc
     2401 cagtggtggg acggatatcg tcagcctgac tattcgtaag aaaacgggta agaatgtcgg
     2461 tagtatttct ttcttggtaa atggaactat catgctgata gcaggtttga cctttggttg
     2521 gaaatacgct ctttattcta tgattaccat ctttgtctct agccgtgtga cagacgcagt
     2581 ctttactaag caaaagcgta tgcaggccat gattgtgaca aatcatccag agaaggtaat
     2641 tgaaaaaatc cataaaaaat tgcaccgcgg agcaaccatg atccacgatg cagaaggaac
     2701 ctataatcac gagagaaagg cagttttaat cactgtcatt acacgtgcag agtttaatga
     2761 atttaaacag attatgacac aagtggatcc aagctccttt gtctctgtct cggaaaatgt
     2821 tcatattcta ggaagatttg ttgagataga taattagtga ttaatactct tcgaaaatct
     2881 cttcaaacca cgtcagcttc accttgccgt atatatgtta ctgacttcgt cagttctatc
     2941 tgcaacctca aaaccatgtt ttgagctgac ttcgtcagtt tcatctacaa cctcaaaacc
     3001 atgttttgag caacctgcgg ctagcttcct agtttgcact ttgattttca ttgagtataa
     3061 aaagatcttg ggaaaagact caatttcagt agaaaaggaa gtaaatcttc ccacattaaa
     3121 acgcatagta tcaagttttt tcaatacctg atagtatgcg tttttgcttt taacgacttt
     3181 ttgcccagtc tcttattttt cgataatttt gtgggcgatc aattgacggt aacaaatttg
     3241 tttattgttt ttattatcat taataatctg gagaatagtt tcaagggaaa cacgaccaag
     3301 ctctaaacta ttgatatcga cataggctgc caagttgagc ttgggattaa ccgagtcaaa
     3361 gctgagaaca gggacatcca gctggtgttt ggcaatatag ttacaaacgc cttcagctag
     3421 gaggctatcg gttgtgatga tagcatcaat ttgtggatcg tgcttgaata atcgcttgct
     3481 aaatttatag cccttttctt ccagaaactc gtcggcaaag tagatgcgat tgttgtcagt
     3541 ggtaagtttg taatgtttaa gcgcctgttc atagcctgtt aaacggtctt tggtcacgaa
     3601 gagcttttta cttcctccga taaaggcaat gcgtttgcag ccttttttga tgaaatattc
     3661 agtcgcatca aaaccagctt gaacattgtc gttgtcgaca agtgggatga aaggagatag
     3721 agatttacct aagataagga aggggaactg ttcttctgcg acgagtttta cgagagggtc
     3781 ttcttcttgg gcatagagaa agattagccc atctacacgc ttgccgtaga ccatttgtga
     3841 aatagcgttg agacgctcct tctcatcttt ccctgttgct atctgaatgg catagtggtt
     3901 ttcagatgcg acttgagaga tgccacgtag aaccgatgga aagaaaggat tctggtagaa
     3961 ggcgtctgag tcatcaggaa gaactaatcc gataacctga gtatagctgc ttaccaagct
     4021 acgagcgttg aggtttgggt ggtagttgag ttccttcata gctttgcgaa cgcgtttttt
     4081 tgtttcgtcg ctaatggttg atttattttg aataacacgg gttacggttg aaggcgaaac
     4141 accagcagcc ttggccacgt ctttaatcgt aacgggcata aaaatctcct atttatggta
     4201 atttggatca cggaaatgtg ttttataaaa gatagtgacc agatacagaa caaaaagaat
     4261 attttgtaca gtaatcaggg ttgtcatatt ttggccaaat aatcccaaaa taagtgtaat
     4321 cagagtcggc aatcctaaac agttcaagat aaagtgatag cactctttaa aggttctaaa
     4381 tgaaaagagg cgtgatttct tagtgatata aaggagaaga ctagctccta gagagacgat
     4441 aaagaaattc aaaccaaaga ggaagctcgc accgagaact aggaagaggc tgatatagat
     4501 acgattttgt tggtaccagt ctttagaaat tgcttgggtc aagctgtctt tgcttttgaa
     4561 actctcagtc tgaatggctc ggtaagagat gcgggtcagt tccttgcttt ccttgctgat
     4621 gactagttca tttgtatcga aatgcagttg caagtcctta ggtaattcct tgatttgact
     4681 tggaccaatc acaacagaag gggcttgact agctgttcca gtataagtta atgtgccatc
     4741 gacaattgta gcatgttcag agagatcctg gacaacctca tttgtcagag gttcatagac
     4801 attatcgata aaagtttcta gcggataggt ctcctgggag ctgttttgga tagcaatggg
     4861 taccatagac aagctgataa ggaagatact ggtaaagaga agttgaaacc agttgagctc
     4921 gaaacgtttg gacaggggct tacgaaaccc ccaaatactt gaaaaatagg aaaatggata
     4981 tggaagcata ggtatctttc taaaaggtgt tttctatatg agtattatta tatagagaaa
     5041 tgcgctttat ttcaagtcta ggagaaaaat tgcttgctac aaggatatta tttacagtga
     5101 caattgctaa taatcaatga aaatcaaagt gcaaaccagg aagctagctg caggttgctc
     5161 aaagcacaac tttgaggttg gagataaagc tgacgtggtt tgaagagatt ttcgaagagt
     5221 ataaaatgaa aaagggtggc ggggataaat tatcccttgt cgccaccact tgtaagtcct
     5281 gaaacaaagt tcttttgtag aaagaagaag agaatacaga ttggaagggc gatgaggata
     5341 gcacctgctg agaagtaggc aatcttcaag tttttcgcat tgttaacgaa ggtttggaga
     5401 cctacggcaa cagtaaagta ttctttctca cgaagcaaga aactagagag gatgtagtcc
     5461 ccgaaaggtc ccatgaaggc ccagagagct tgtacggcaa ccattgggcg aacaagtggt
     5521 agaacaattt gccagaagcg gcggaagtgt cctgcaccgt ctagttttgc agattcgtct
     5581 aaagacattg gcactgtatc gaagtagcct ttcatgagcc aagcattcat cgggatacca
     5641 ccaccaacgt agaggaagat gaggaaccag ttgtggttaa gggcgttcaa cataagcgcc
     5701 ataacgaaga aggctgtcaa agcggccata gttggcacca tttggatgat caagaagaag
     5761 accaaacttt gtttacgagc caagaagttg taacggctgt aagcataacc agcaagtacg
     5821 atgatacttg tttgaacagc catggtaatt aaggcgataa tcaaagtgtt gaggtaccaa
     5881 gtaccgtaca aggtttcagt gaagaggcct ttaaagttat caaaattgag gtcgatatta
     5941 gtatctagtt taaaggctga gacgttacct gctttaaagg ctgacataat ggtaatcaac
     6001 agtggataga taattacaat tgatagacca atcaggtaaa ggtaagtaag gctttgagtc
     6061 agtctacgtt tgagtttaat tgagttattc atcttagacg tcctccatat caaatgcgtg
     6121 tagtttcttg aatgcgatca tagagattga gatgacaatg atagagataa tcaaggtaac
     6181 agctgccgcc attgagtatt gaggagatgt acctgttgtc aaacggtaga tccatgagat
     6241 caagatatcg gttgaaccag ctccacctcc gacactacca ggtcctccac cattgaagag
     6301 gtacatgata gagaagttgt taaagttgaa ggtgtattgg ctaatcaaag taggtgccgc
     6361 aacagccaaa atcattggga aagtgatgtt gcggaatttt tgccaagcgt tggcaccgtc
     6421 aatataagct gcttcgtaaa ggtcgttagg aatagattgc aagataccca aagtcaaaac
     6481 gtagatgtat gggaatccga gccaaccttg catcataatc aaggcaatct tagtccaagt
     6541 tgggtctgtt ttccaaggaa taagagctcc atcaaggaaa ggaaggaatt tagccaagat
     6601 tggcaatact tgagtgttga tagcaccgac actatcgtta aacatgtttg agaatgtcaa
     6661 gatagtgatg aaggctggga cagcccaagg aagaaggaaa ataacaccaa agatacgttt
     6721 tcctttgata aatggttggt tggcaatgat agctgtgaag ataccaatta cgatttgtaa
     6781 agtagaagct gccaaagccc aaatgatagt ccaagaaaga acagaaccaa aggcagaacg
     6841 gaaggtactc aagctccaaa tgtttgtaaa gttggtcaaa ccaacccagt ccaacaactt
     6901 gtttggtggc aagtgttgga agtcgtagtt ggtaaaggcg atcatcaagg ttacgataac
     6961 tgggaagata atcgcgaagg tcatggcaac ataagatgga atgatcaaga ggtaagggaa
     7021 gccattttca taaatccctt tgatcatgtc tttgagtgtg cgtggaactg gaattccatt
     7081 gttaatgcgt tttgcaatcg tatgtgcatc tttgatattt gagaaataaa agagtacata
     7141 aacgattaca aagattagat ggaaggcacc acgaatcagc ataaagaggg aattatcacg
     7201 acctggtttg ttaccaagag tgatgaggtt gctcaattca ggggttgcaa gtgctaggaa
     7261 gtaaaggaca aatacgatgg ttacaccaag gaagataaaa cctttggctt tttgtttatt
     7321 gtaaatctgt cctaacccag gaatgataga cagcagggct gctttactag gttgttgctt
     7381 ttccatatta actcctttca taggatacta cttttttata agaaagctaa attatttgtg
     7441 tctctattga taaattcaaa gggggatttg atttccaccc cccttgaaca aattttttat
     7501 tcaccaaatt tttgtttgat tgtttctttg atcaatgtta cagcatcgtt agcagctgtt
     7561 ttagcatctt tttgaccact tacagcatca aagagcatat ttttcgctgg atcccaaact
     7621 gcagacattt gagagatgtt tggcagtggt tgagtgttct tgaactgttt gataacagct
     7681 gttgtcaact catcgttttt accttcagcg tatgaacgag cctcagtatt agctgggatt
     7741 tcgttagtct tatcatataa tactttttgt tgttcagttg caacaaggaa gtctacaaat
     7801 ttttgagaag cttcaaggtt cttaacggct tgaggaatga cccaagcttt accaccaccg
     7861 aatgcagcat attcttttcc atttggaaga gttgggatag ttgcaactcc gtagtttact
     7921 ttagcatctt taaaggcttg agctttccaa ggtccgtcga tgatagcagc tgttttacct
     7981 tcttggaatt gagtttggat taagtttcca gcaccttctg tatcttgcat acctttaggc
     8041 catttttcgt accaagattt agcgtaattg atacctacga tagaaccgtc gtttgcaaga
     8101 ccgatgtctt tagcgtcttt accgttttgg ccaaagacgt aagcaccgtt accggcaaga
     8161 agtccatatg tatagtagaa gtttgtccag tcagctagga aggcagtagt tttaccatct
     8221 tcaccagcga atgcgtattt gctatcttta gcaaggtttt ccaagtcagc aaatgttttt
     8281 ggagcatctt tcaccaagtc tttgttgtag tacataacaa gtgactcgat aacggcagga
     8341 gcaccgtaaa ctttaccatt agcagctgtt acaagagatt tagttgtgtc gtctgtttta
     8401 gcaccatcgc tcaatttcac ttctgaaagt tgtccgtcag aaccaaggct acctacacgg
     8461 tcgtatggag ccatcataac atcagggaca ttaccagatt ggttgtcaag agaaagttta
     8521 tcaagacctc ctagagcatc accagtttta agagtgactt ttactccagc ttctttttca
     8581 taagctttag caacctcttc aatatagctc ttatatccct cgtctacata tacagtgagt
     8641 tctttgactt cagatgaacc agaatcagca ggcttatcag cagttttgct tccgcaagct
     8701 accaaaagca agctagcaag tgtagcagtt ccaagcaccg cagtgctctt cataaattta
     8761 gatgacatag tgtattcctc ctaaagaata gcaagtttta ttgataagga aacgcaaacg
     8821 ttttccttta tgagcttagt atagcacaaa tagaaaacgg ttgcaagtat tttttgtaaa
     8881 aattttaaaa aatttttaag cttgatttga tagcataatt ttgcatttta atagaagaaa
     8941 agtatgaaat agataagaat acagtatttt taataaaaat atatggaatc tgtctaaaga
     9001 gggaaatcag gaggggctac ctccctgttt ctaatctata tctattttct atagacgttt
     9061 gtgctttgtt acatataaat tcaagctccc taaggaggtt ttatactttg tagactgtct
     9121 cgtgagagtc aaaccttagg attagatagt tttaagttat ctttgctaaa atgagttagt
     9181 ttccttaaaa ataatcaaaa aagtttttta aaaacgcttg caattatgct tgaaaaggag
     9241 tatacttata agtaacgcaa acgtttgcgt ctgcaaaata cgcaacgttc cattatttta
     9301 acacacgagg tgctattatg aaaaaacgtc aaagtggtgt gttgatgcac atctcttctc
     9361 ttccaggagc ttacggaatc ggatcatttg gtcaaagtgc ttacgacttc gttgatttct
     9421 tggtccgtac aaaacaacgt tactggcaaa tccttccatt aggagcaact agttacgggg
     9481 attctcctta ccaatctttc tcagccttcg caggaaacac tcattttatc gatttagata
     9541 tcttggtgga gcaaggtttg ttggaagcaa gtgaccttga aggagttgac tttggtagcg
     9601 atgcgtctga agttgactat gctaaaatct actatgcacg tcgtcctctt ttagaaaaag
     9661 cggtgaaacg tttctttgaa gtcggagatg ttaaagattt tgagaaattt gctcaagaca
     9721 accaatcatg gcttgagctc tttgctgagt atatggctat caaagagtat tttgacaatc
     9781 ttgcttggac tgaatggcca gatgcagatg ctcgtgctcg taaagcttca gcacttgaaa
     9841 gctatcgtga gcaattggca gacaagttgg tttaccaccg tgtgactcaa tacttcttct
     9901 tccaacaatg gttgaaattg aaagcttacg ctaacgacaa ccacatcgaa atcgttgggg
     9961 acatgccaat ctacgtagcg gaagattcaa gtgatatgtg ggcaaatcca catctcttca
    10021 aaacagatgt caatggtaag gctacttgta tcgcaggatg cccaccagat gagttttctg
    10081 taactggtca gctttggggt aacccaatct atgactggga agcaatggac aaagacggct
    10141 acaaatggtg gattgaacgc ttgcgtgaaa gcttcaaaat ctacgatatc gttcgtatcg
    10201 accacttccg tggcttcgaa tcttactggg aaatccctgc tggttccgat acagcagcac
    10261 ctggtgagtg ggtgaaaggt ccaggctaca agctttttgc agccgttaag gaagaacttg
    10321 gtgagctaaa catcatcgca gaagaccttg gcttcatgac agatgaagtg atcgaattac
    10381 gtgaacgtac tggtttccca ggaatgaaga ttcttcaatt tgccttcaac ccagaagacg
    10441 aaagcattga tagcccacac ttggcacctg ctaactcagt tatgtacaca ggaacacacg
    10501 ataacaatac ggttcttggt tggtaccgta atgagattga tgatgcgact cgtgagtaca
    10561 tggctcgtta cacgaaccgt aaagaatacg aaacagtggt acacgctatg cttcgtacag
    10621 tattttcatc agttagcttt atggcaattg caactatgca agatttacta gaattggatg
    10681 aggcagctcg tatgaacttc ccatctaccc ttggtggaaa ctggtcttgg cgtatgactg
    10741 aagatcaatt gacaccagct gtcgaggaag gtttgcttga cttgacaaca atttatcgcc
    10801 gaattaatga aaatttggta gatttaaaga aataagacaa taatcaggag acaactaaac
    10861 atgttatcac tacaagaatt tgtacaaaat cgttacaata aaaccattgc agaatgtagc
    10921 aatgaagagc tttaccttgc tcttcttaac tacagcaagc ttgcaagcag ccaaaaacca
    10981 gtcaacactg gtaagaaaaa agtttactac atctcagctg agttcttgat tggtaaactc
    11041 ttgtcaaaca acttgattaa ccttggtctt tacgacgatg ttaaaaaaga acttgcagct
    11101 gcaggtaaag acttgatcga agttgaagaa gttgaattgg aaccatctct tggtaatggt
    11161 ggtttgggac gtttggctgc ctgctttatc gactcaattg ctactcttgg tttgaatggt
    11221 gacggtgttg gtcttaacta ccactttggt cttttccaac aagttcttaa aaacaaccaa
    11281 caagaaacaa ttccaaatgc atggttgaca gagcaaaact ggttggttcg ctcaagccgt
    11341 agctaccaag taccatttgc agactttact ttgacatcaa ctctttacga tattgatgtt
    11401 actggttatg aaacagcgac taaaaaccgc ttgcgtttgt ttgacttgga ttcagttgat
    11461 tcttctatta ttaaagatgg tatcaacttt gacaagacag atatcgctcg caacttaact
    11521 ctcttccttt acccagatga tagtgaccgt caaggtgaat tgctccgtat cttccaacaa
    11581 tacttcatgg tttcaaacgg tgcgcaattg atcatcgacg aagcaatcga aaaaggaagc
    11641 aacttgcatg accttgctga ctacgcagtt gtccaaatca acgatactca cccatcaatg
    11701 gtgattcctg aattgattcg tcttttgact gcacgtggta tcgatcttga cgaagcaatc
    11761 tcaattgttc gtagcatgac tgcctacact aaccacacaa tccttgctga agcgcttgaa
    11821 aaatggcctc ttgaattctt gcaagaagtg gttcctcact tggtaccaat catcgaagaa
    11881 ttggaccgtc gtgtgaaggc agagtacaaa gatccagctg ttcaaatcat cgatgagagc
    11941 ggacgtgttc acatggctca catggatatc cactacggat acagtgttaa cggggttgca
    12001 gcactccata ctgaaatctt gaaaaattct gagttgaaag ccttctacga cctttaccca
    12061 gaaaagttca acaacaaaac aaacggtatc actttccgtc gttggcttat gcatgctaac
    12121 ccaagattgt ctcactactt ggatgagatt cttggagatg gttggcacca tgaagcagat
    12181 gagcttgaaa aactgttgtc ttatgaagac aaagcagctg tcaaagaaaa attggaaagc
    12241 atcaaggctc acaacaaacg taaattggct cgtcacttga aagaacacca aggtgtggaa
    12301 atcaatccaa attctatctt tgatatccaa atcaaacgtc ttcacgagta caaacgccaa
    12361 caaatgaacg ctttgtacgt gatccacaaa taccttgaca tcaaagctgg taacatccct
    12421 gctcgtccaa tcacaatctt ctttggtggt aaagcagctc cagcctacac aatcgctcaa
    12481 gatatcattc acttgatcct ttgcatgtca gaagttattg ctaacgatcc agcagtagct
    12541 ccacacttgc aagtagttat ggttgaaaac tacaacgtta ctgcagcaag cttccttatc
    12601 ccagcatgtg atatctcaga acaaatctca cttgcttcta aagaagcttc aggtactggt
    12661 aacatgaaat tcatgttgaa cggagctttg acacttggta ctatggacgg tgctaacgtg
    12721 gaaatcgctg agttggttgg agaagaaaac atctacatct tcggtgaaga ttcagaaact
    12781 gttatcgacc tttacgcaaa agcagcttac aaatcaagcg aattctacgc tcgtgaagct
    12841 atcaaaccat tggttgactt catcgttagt gatgcagttc ttgcagctgg aaacaaagag
    12901 cgcttggaac gtctttacaa tgaattgatc aacaaagact ggttcatgac tcttcttgac
    12961 ttggaagact acatcaaagt caaagagcaa atgcttgctg actacgaaga ccgtgacgca
    13021 tggttggata aagtcatcgt taacatttct aaagcaggat tcttctcatc tgaccgtaca
    13081 atcgctcagt ataacgaaga catctggcac ttgaactaat actcttcgaa aatctcttca
    13141 aaccacgtca gctttatctg caacctcaaa gcggtgcttt gagcaacctg cggttagctt
    13201 cctagtttac tctttgattt tcattgagta taagatacaa atttatacta atacatcttg
    13261 taaaaaagcg agtttcgatt gaaattcgct tttttaatga tgtagatttg ggtcaatctt
    13321 gtctaaaaat agggaaatcc tagatacagt gaaggcttta aatgctggtt tttactgtcc
    13381 tcagccttat attttttcgt agttgattac ctcatacctc ttgtattcgc ttacataaag
    13441 tattataata taattgtagg aaagaaggtg tttttatgat atacacactt aaattggtgt
    13501 tgtttattac ctttcttgta ataagcttgt tacctgataa gatttttgga aaaaataaaa
    13561 aaatttggaa aatagttttt gcaatattga cggcagtggc agcattgtca tttatgtact
    13621 aagttatttt aagaatgtag ggaaataaac cctacattct ttttagtttt ttctgttttc
    13681 taaattctat ttatccaagc gcttcaacat ttcttgcttc ttcgcttcaa gttctgcacg
    13741 cttttcttcg atttcggcat gttttttctc gagttcagaa caacttgcac cattgctaaa
    13801 ttcttttcgc catcaggaga tagggtgagt cgacatgtct attactcacc caaagcagtc
    13861 ctacaaagca ggaattttct gttacttttt tggaaatagt aacgtttata cagctttgac
    13921 acttcgtatc aaagcgccaa acacactccg agggatttac agaaagcaga aaaggaatga
    13981 tctggtataa gatcattcct tttctctctt tttctttaag taattatata caatgtacga
    14041 cgaagtcgtc attgcaatgc tgatccacca cctaaaggaa actttaaaca acattgataa
    14101 gataaagaat ataaacaacg aaaatacgtt atacccaatt aattttattg tatatctcat
    14161 gattaaaagt taatccttcc gttgttagga atggcatcat ttttatccca taattgtgct
    14221 aaataagtcc ccggtgataa taaatttata gcaaattcta aagcaacatc atttacaaac
    14281 caactaccta gatatctaga aattgctgaa cgaatagcac tttttgctgc atgttttcct
    14341 tttactttaa ttagatttgc aaggcctgca gtagttcctc ctaatgctaa agctattgca
    14401 gtatctaata gagcacccat ttgattaact gtaatacctt gccaagctgc tctaaatgga
    14461 gagtatgtag gtgggattgt ataatcgcct tgtaattgtc ggttaattac ttctttgatc
    14521 cattgttgtg agacgtctgg atgaaaagat tggatttcgt ttgcaagtgt attgatttgt
    14581 tcttctgtta gagaagtgac aggttgaagt tccatatttg tttcaatttg tgatacttgt
    14641 tcagaagcgt atacagctga aacacttgga atcgctgata caattaacac aattgacgtc
    14701 aaaaaaaccg aaataaattt cattaatttg ttcatgagct tttctccttt ttatttgtat
    14761 ctgcttacat tttatcatat gctgttatta tagtcaaaaa aatatgctat tatgttaaaa
    14821 aaatattttt caaaatataa atggacggat ttattttgga ttttatttgt tattttgacc
    14881 tgcctctata ttggtaacca tgatttgttt actctcaatc atcaagaatt ctcttttcgt
    14941 ggtagcgttt ggggtctggt actggcctta tatcacttac tattcattga taagtttgtt
    15001 atatcgaatc gaaaataaag attagagcta tgcttgactg tgtactttta ggattgattt
    15061 tggaggaaga ttttgtctct attatttatt attttaaatt tatttatttt gtataagatc
    15121 tattctttga ggcgggaaaa atcgaaatac ttgatttata cggcctatat catatttggg
    15181 gtaaatgtag tatatggtat tcaatggtta ttaagagaac tgatttcaac tatttcccct
    15241 taatgtgtat ggaaactata acaaaacgca taagattaag gtttgtgtac ctgttcttat
    15301 gcgtttttat aatattgaag attttcggta agttttatac ctttgggaag tctagtttag
    15361 aatgctttcc caaccaaaat ctctgcttcg atggtaatct ctgtcagttg gctccagtca
    15421 atcttgctct ggtcaacgtg aaagaggaga ggagtcatac tgagtagggc ttggagttgt
    15481 tctgctgtga tagtcttagt cagagaggca gtttgactag ataggatggt aaagtgttcc
    15541 tggaaatgct ccttgatatc ttggttagaa tactccttgt ttgtcagctg gtcctgtact
    15601 ctttgacgga tttctttgag gtgattttca gttgggataa cctttatcaa gataccgtct
    15661 ttggataaaa cgcgacgaaa ttctccatag ttggcaggtg agaagatatc aagcagaata
    15721 tccatgttag cgtcttttat aggaagtcgt gccaagtcgc caacgaacca attgactgcc
    15781 cagttgggtt cacttttagc agcgatttgg actgaatctt tggagatgtc aaaggcatag
    15841 aaagtttttt cagagtgact ttcttgtagt ttgcgagaat agaatccttc accacaaccg
    15901 atatccaaaa ttgttgtggt agtttttgag cttgcaagca agtcagatac agcatctaag
    15961 atagcttggt aaaagccggc ttctaggatt tgttgacggt tttgaaaatt ttccttgtca
    16021 tagttagcag attgcttgat ttgaggtgct agattgacat agccaaattt cgccaagtca
    16081 aaagaatgac ggttgcagca cttgaaatta gtctctaaca gagtcagatt ttcttgacag
    16141 ataggacagg caaaggcagt cgcagaagca aaacgctgaa gtttgggctt gagatttgta
    16201 ttcatagttt aagtgtatca aaagaggcaa atgaaagcaa ctttaggaat aagtagattc
    16261 agtagaaata aaactaattc tccaatttat agaatgaaat tgcttttata tctaaattgc
    16321 tatataataa tgataaggag gaaataagta tgttaaaaga agtattaacc gttgcaaaag
    16381 ttgcgaaaaa atcttcatta tttttgggtg gtgtcgcatt tggtaccctt ggtttgaaaa
    16441 tcttagcaag taaggaagct aaaaaaggtt attctaaagc tttggctaag gcttacaagt
    16501 tgaaagacga gctagatgca tctgtttctg ttgtgaagca acatggagac gatgtcttgc
    16561 aagatgccaa atatttgtac gagcaagaga aaaaagaaga gcaattagat agccttatag
    16621 gtgaataata tgtcttttaa agtgctacat agaggatacc aacatatccg actatcatct
    16681 tctttttcac ttaccttgga tattcaagac tatcttcgtt ccttggcgag agatgaaaag
    16741 gggattgagt ctatccagtt ttacatggat caacagcact ttactctacg cataaaagaa
    16801 ggcttttctg tattagataa tgcagaagcc tttttaaaaa gaattgataa agggaaagtt
    16861 tctgagttga tgactcttcc cattcgtaga gaagagagtg cttattctat tgtttcaggt
    16921 gcagcggtta agcgtgtact ttttcgtagt tttgtgccgt atcctattcg ctatatatgg
    16981 acttgttatc aggctttggg ttatattaga gaagcctatc aaacactagc gcgtaaggaa
    17041 ctaacgatgg aagtcttgga ctgttcggct attttattgt ccttgtttat gaaccaatcc
    17101 aagacagcta gcaatatcat gtttatgctt gatttgggga atcatttaga tcagtggtcc
    17161 ttgaaaaaaa ctgcaacaga tttagaacag agtcttcttg caaaagagag cgatgtattc
    17221 ctagtacagg gcgatacggt tgttagtatc aagagttccg atgttcaaat aggagatgtc
    17281 ttgatcttat ctcaaggaaa tgaaattctg tttgatggac aagtagtttc aggtttaggt
    17341 atggtcaacg aaagttcctt gacaggagag agttttccag ttgaaaaaag agagtctgat
    17401 ttggtttgtg caaatacagt attagaaact ggagagttac gcattcgtgt aacagataat
    17461 cagatgaaca gccgtatttt acagctgatt gagttgatga agaaatctga agaaaacaag
    17521 aaaacgaaac aacgctattt catcaagatg gcggataaag tcgtcaaata taatttcttg
    17581 ggggctgggc tgacttacct attgacaggt tctttttcta aggctatttc tttcctattg
    17641 gtcgatttct cctgcgcttt gaaaatctct actcctgtag cttatttgac agttatcaag
    17701 gaagggttga accgtgaaat ggtgattaag gatggagatg ttctggagaa atatctggaa
    17761 gttgatactt tcttgtttga taagacagga acaatcacaa ctagttatcc tatagttgaa
    17821 aaggtgttac cttttggaga ctatagtgag gaagatattc tcagaatcag tgcctgtctt
    17881 gaggaacaca tttatcatcc tattgctaat gccatcgtca agcaagctga gatagaggga
    17941 attgaacatg aggaaatgca tgggaaactc caatatatcg caagcaaggg gatcaaatct
    18001 catatagatg gccaaccagt tcttattggg aattatgtct tgatgcagga tgagcagatt
    18061 catatcagtt cagaacaaaa tgctttaatt gaagagtaca agagtcacta caatctctta
    18121 ttcttggctt atcaaaatga attgattgga atgttctgca ttcatactcc tttgagaaaa
    18181 gaagcaaaaa cagccttgga taaacttaag gcacagggga aaaaattgat tctggcaaca
    18241 ggagacacct tgattaggac agaggaatta gtcaaagatt tgccctttga tcaggtctat
    18301 acagacttga aacctgatgg gaaatttgag ttagtagaga aactgcagaa agcaggtcac
    18361 actattttga tggttggaga tggattgaat gactcagcgg ctttaactct atcagatatc
    18421 ggtgtggtga tgaatgagag tgcagatatt tctaagcaga tgagtgatat cttattgtta
    18481 gataatcgtt tggatttctt ccaagagttg gattcgttat catcatcttt gcaaacactc
    18541 atcaagaaaa atattcagga taccgttgtc gtaaatagta gtttgattgg ctttggctta
    18601 tttaattggt tcagtccttc aaatctctct atcttacata atctaacaac cttgcgcatt
    18661 gtcctgcgta gcctgtctat taaaaataga tagatgagag ttattaacag caaaaaggag
    18721 ttacctttct cgaggttaac tcctttgttt atagataaat gttgaatagt ttagtaagtc
    18781 aatacaaagt attttttctt cccacgacgg ataacagtca gttcattttc taacttatca
    18841 gcgtcactca agacatagtc aaggtcttgg atgcggtcgc cgtttacgta gatggctccg
    18901 ttttggacgt cttcacgggc ttggcgtttt gagttaacta taccagatga gacgagcagt
    18961 tccacgatat tgttgttttc gtctgcctgt acttggtagt ttggcacacc acgaagtcct
    19021 tgtttgagct ctttgacaga aaggtttttg atgtttcctg caaagagttg ctcagtgatg
    19081 ttaagtgctt ctttgtaggc ttcttctccg tgaacaagtg taacaacttc acgagccaag
    19141 actttttgag ccaagcgttc gtgtggcgct gcttcaaatt gtttacgaat atcttcaatc
    19201 tcatcaagtg acaagaaagt aaagattttc aagaagcgaa cagcgtcagc gtccatcacg
    19261 ttcatccaga attggtacat ttcgtatgga gaagtctttt cgggattgag ccagatggca
    19321 tttccttctg atttaccaaa ttttttacca gttgcatctg tgattagtgg aacagtgata
    19381 acgtgaccag tcttgtccgc cttacgacga agcaattcgg taccagctgt catatttccc
    19441 cactggtcag aaccaccgat ttgaagagtg acattatggt cttggttaag gacgaagaag
    19501 tcgtaccctt gcatgatttg gtaagcgaac tcagtgtaag aaattcctgt ttcgatccgt
    19561 tttttaacag attccttact catcatgtag ttgaccgtga agtattttcc aatgtcacgg
    19621 aggaagtcaa tgaagctgat gctgccaaac cagtcgtagt tgttgaccat gacagccttg
    19681 ttttcgccat tttcaaagtc aagaaaacga gaaagttgtc cttggataga cttgacccag
    19741 ccatctactg tgtcttttgt ttggagacta cgttcagcat ctttgaagga cggatctccg
    19801 atgagacctg tagcaccgcc aacgagcgca taaggtttgt gacctgctag ttgcaagcga
    19861 cgacttgtca agattgcgac aaggtggcct aggtgaaggc tgtcagcagt tggatcgtag
    19921 ccagtataat aagaaacttg accttcttct agggctttac gcaaagcttc ttcatcagtc
    19981 gtttgaaata tcaaaccacg ctcttttagc tcatcaaaaa tgtgcatgtg tcttttctcc
    20041 tttttaaaat tattgtttct acctattgta tcacaaactt gggcaatagc ctagcagaat
    20101 agggaagaaa gtggctattt tattgaaaat ttcccttttg tggtataata gataaagtga
    20161 ggaaatccat gcaaaatcaa ttaaatgaat taaaacgaaa aatgctggaa tttttccagc
    20221 aaaaacaaaa aaataaaaaa tcagctagac ctggcaagaa aggttcaagt accaaaaaat
    20281 ctaaaacctt agataagtca gccattttcc cagctatttt actgagtata aaagccttat
    20341 ttaacttact ctttgtactc ggttttctag gaggaatgtt gggagttggg attgctttgg
    20401 ggtacggagt ggccttattt gacaaggttc gggtgcctca gacagaagaa ttggtgaatc
    20461 aggtcaagga catctcttct atttcagaga ttacctattc ggacgggacg gtgattgctt
    20521 ccatagagag tgatttgttg cgcacttcta tctcatctga gcaaatttcg gaaaatctga
    20581 agaaggctat cattgcgaca gaagatgaac actttaaaga acataagggt gtagtaccca
    20641 aggcggtgat tcgtgcgacc ttggggaaat ttgtaggttt gggttcctct agtgggggtt
    20701 caaccttgac ccagcaacta attaaacagc aggttgttgg ggatgcgccg accttggctc
    20761 gtaaggcggc agagattgtg gatgctcttg ccttggaacg cgccatgaat aaagatgaga
    20821 ttttaacgac ctatctcaat gtggctccct ttggccgaaa taataaggga cagaatattg
    20881 caggggctcg gcaagcagct gagggaattt tcggtgtaga tgccagtcag ttgactgttc
    20941 ctcaagcagc atttttagca ggacttccac agagtcccat tacttactct ccttatgaaa
    21001 atactgggga gttgaagagt gatgaagacc tagaaattgg cttaagacgg gctaaggcag
    21061 ttctttacag tatgtatcgt acaggtgcat taagcaaaga cgagtattct cagtacaagg
    21121 attatgacct taaacaggac tttttaccat cgggcacggt tacaggaatt tcacgagact
    21181 atttatactt tacaactttg gcagaagctc aagaacgtat gtatgactat ctagctcaga
    21241 gagacaatgt ctccgctaag gagttgaaaa atgaggcaac tcagaagttt tatcgagatt
    21301 tggcagccaa ggaaattgaa aatggtggtt ataagattac tactaccata gatcagaaaa
    21361 ttcattctgc catgcaaagt gcggttgctg attatggcta tcttttagac gatggaacag
    21421 gtcgtgtaga agtagggaat gtcttgatgg ataaccaaac aggtgctatt ctaggctttg
    21481 taggtggtcg taattatcaa gaaaatcaaa ataatcatgc ctttgatacc aaacgttcgc
    21541 cagcttctac taccaagccc ttgctggcct acggtattgc tattgaccag ggcttgatgg
    21601 gaagtgaaac gattctatct aactatccaa caaactttgc taatggcaat ccgattatgt
    21661 atgctaatag caagggaaca ggaatgatga ccttgggaga agctctgaac tattcatgga
    21721 atatccctgc ttactggacc tatcgtatgc tccgtgaaaa gggtgttgat gtcaagggtt
    21781 atatggaaaa gatgggttac gagattcctg agtacggtat tgagagcttg ccaatgggtg
    21841 gtggtattga agtcacagtt gcccagcata ccaatggcta tcagacctta gctaataatg
    21901 gagtttatca tcagaagcat gtgatttcaa agattgaagc agcagatggt agagtggtgt
    21961 atgagtatca ggataaaccg gttcaagtct attcaaaagc tactgcgacg attatgcagg
    22021 gattgctacg agaagttcta tcctctcgtg tgacaacaac cttcaagtct aacctgactt
    22081 ctttaaatcc tactctggct aatgcagatt ggattgggaa gactggtaca accaaccaag
    22141 acgaaaatat gtggctcatg ctttcgacac ctagattaac cctaggtggc tggattgggc
    22201 atgatgataa tcattcattg tcacgtagag caggttattc taataactct aattacatgg
    22261 ctcatctggt aaatgcgatt cagcaagctt ccccaagcat ttgggggaac gagcgctttg
    22321 ctttagatcc tagtgtagtg aaatcggaag tcttgaaatc aacaggtcaa aaaccaggga
    22381 aggtttctgt tgaaggaaaa gaggtagagg tcacaggttc gactgttacc agctattggg
    22441 ctaataagtc aggagcgcca gcgacaagtt atcgctttgc tattggcgga agtgatgcgg
    22501 attatcagaa tgcttggtct agtattgtgg ggagtctacc aactccatcc agctccagca
    22561 gttcaagtag tagttctagc gatagcagta actcaagtac tacacgacct tcttcttcaa
    22621 gggcgagacg ataattctct aaatgaagtg gccaatcaat ggattgccac ttttttcctt
    22681 tcgtgttaca ataagggtat gaatcagtat cagaaaaaga ttgttaacgg aaaaatttat
    22741 tcgctcctat ccggcttaat atggggaatc tgtggaattt taggagagta cttctttact
    22801 cattatcagg tgtcttcggg ctggattacc tctatgcgtt tgacactggc agggagtctt
    22861 gtactcattt ggtctgcaat acaattaaaa tcgcaagtgc tagatatttg gcgagacaag
    22921 aaaaattacc tgcccttttt agcctatgct attttgggga ttttttcagt tcagtatttt
    22981 ttctatctct gtttagaata ctcaaatgct acgacagcaa ctattttaca gtttattagc
    23041 cctgtcttta tcctctttta caatcgcttg gtttatcaaa aacgagcgtc aaaaagcgct
    23101 gttttctatg ttttggttgc catgctgggt gtttgcttga tggcgacaaa gggagatctc
    23161 tctcagttat ccatgacgcc gctagctctt ataacaggtt tgctgagtgc catgggtgtt
    23221 atgtttaatg ttatcttgcc ccaacctttt gctaagcgtt atggttttgt tcctacggtt
    23281 gggtggggga tgattttggc aggtttgttt agcaatgtcc tctcgccggt ttatcagctt
    23341 tcctttactc ttgatatttg gagtatcttg atttgcctca ttatcgcttt ctttggaacg
    23401 gcttttgctt ttttcatttc catgaaggct gtgtccttgg tttctccttt ggtggtttcc
    23461 gttatcagtg ccagtgaacc tctctcttct gctctcttga gtgttttgtt cttaggatta
    23521 gtagtggatt ggtccctcct tctagctata gccttgatta ttttacccat gatatttttg
    23581 tctatagaag aagcgaaaga aagtagataa aaagtctttt cttaattcta aaagtgtgct
    23641 atactagaat agtcaataaa acacggggag atagttgtga agagtatttt taggttgtat
    23701 cggtaggggc ttgcttttac cgacagcacg ttttatctca catccctaaa aaatcatacc
    23761 taaaaacaga caaaaaggtt tcaaatttga ggcctttttt ggtagaatag gtatcattat
    23821 aacgaaccag gaggcaccta tgactgctac aaaaatgaac gctcaagaaa ttatccaatt
    23881 tatcgccaat gctgaaaaga aaaccagtgt caaagtaacc tttgaggggc aactcgcaac
    23941 tgctgtgcct agctctgttg tcaaactagg aaatgtccta ttcggagact ggaaggacgt
    24001 ggctccgctt cttgaaggtt tggtagaaaa tcaagattat gttgtcgagc aagatgctcg
    24061 taattctgca gttcctttgc tagataagcg tgctatcaac gctcgtatcg agccaggtgc
    24121 tattatccgt gaccaggtgg aaattggtga caatgctgtt atcatgatgg gagctgttat
    24181 caatatcggt gctgaaatcg gtgctggaac catgattgac atgggtgcca tccttggtgg
    24241 ccgtgctatc gttggaaaaa atagccacgt tggtgcaggt gcagttttgg caggtgtgat
    24301 tgagccagct agtgctgaac cagtctgtgt cggagacaat gttcttatcg gggctaatgc
    24361 agtggttatc gaaggagtcc aaatcggtag tggttcagtt gtcgctgcag gagctatcgt
    24421 tacccaagat gtcccagaaa acgtggtagt agcaggtgtt ccagctcgta ttatcaaaga
    24481 aattgatgcc caaactcaac aaaaaacagc gctagaggat gcgcttcgta ccttgtaatt
    24541 gtaaaagtaa aaaagaggcg gaaccctttt tccagcctct ttctgctata tcggaggaca
    24601 gatagatgtt agatttgatt cagactagac gagctttaca ccagattcca gagattggct
    24661 tggaggagtt caagactcag gcttatttgc tggatgtgat tgagaaattg actacgggca
    24721 aggattttgt tcaaattcgt acttggcgga cagggatttt ggtctacctg cagggaagtc
    24781 agccggagcg aaccattggt tggcggacag atattgatgg cctgcctatc gtcgaacaaa
    24841 caggtctacc ttttacttct caacaccaag gtcgcatgca tgcctgtggt catgattttc
    24901 atatgaccat tgccttaggc tgtcttgagc gcgcccttga ggagcaaccc aagaataatc
    24961 tgctctttct atttcagcct gctgaagaaa atgaagctgg tggtatgctc atgtatgagg
    25021 acggtgcttt tggagattgg ttgccaaacc aattctatgg tctccatgtt cgtccagatt
    25081 tgaaagtcgg ccagattgcg actaatactc atacactctt tgcaggaact tgcgaggtga
    25141 agatccgttt caaaggtaaa ggtggccacg cagcttttcc tcatgaagcc aatgacgcct
    25201 tggtggcggc tagttacttt gtgacccaag tgcagtcagt tgttagccgc aatgtcaacc
    25261 caatcgaggg agcagtggtg accttcggcg tttttcaagc tggaaccacc aacaatgtca
    25321 tcacagacac agctttttta catggaacta ttcgtgcctt gacacaggac atgagtctct
    25381 tggtgcaaaa gagggtaaaa acagttgcag aaggggttgc agctgccttt gatatggaag
    25441 tcgaagtgga actcaaacaa ggtggatacc tacctgttga gaacaatcca gccttggcgc
    25501 gtgaactgat ggacttcttt gacgagaaag acggaatcga gttgattgat atcgaacctg
    25561 ctatgacagg cgaggacttt ggttatctcc tttcaaaggt tgatggcgtg atgttttggc
    25621 taggtatcga tagtccctac gctcttcatc accctcaaat gagtcctaag gaagaagtct
    25681 tagctattgg ggtggctgcg gtgtctagtt tcctgaaaaa gaaggcagca gaatagaggg
    25741 cttgtctatg aaatcggaat tacgcaagca agtcttgcat gaaatgaagg ctatatctca
    25801 agagcaaaaa caggctatag accaagcttt aaccgagcga attttacaac acccctttta
    25861 tcaagaagcc aaggtcatcg caacctacct ctctttttct catgagtttc aaacgcggga
    25921 actgattgag caggcgctga aggacggcaa gaaggttttg acacctaaga cctatcccaa
    25981 ggggcgcatg gattttgtgg tctatgatcc gcaacagttg gtaaaaactt cctttgcctt
    26041 actggagcca cagggagatt tggaagtagt ggatgcatct cagattgatt tgattcatgt
    26101 tcctggcctg gcttttacga cggatggata tcggattgga tatggtggag gttattatga
    26161 ccgctatctg gaacattttt ctggtcgtgc tttgagtacg atatatcctt gtcaaattca
    26221 ggactttata cctgaaaacc atgatattcc tgttcaggag gtattaattg atgaaggaaa
    26281 tctttgatag acgttaccct gtgacgagtt tcttcctctt agtgacggcc ttggtatttt
    26341 tactaatgtt ggtcactgca ggcggaaact ttgacagggc agatacatta tttcgatttg
    26401 gagccatgta tgggccagct attcgcctct ttcccgagca ggtttggcgt ctcttgtctg
    26461 ccatttttgt tcatattggg tgggaacatt tcattgttaa tatgctttca ctttattatc
    26521 ttggaaggca ggtagaggag attttcggtt ctaagcagtt tttctttctc tatcttttat
    26581 caggaatgat gggcaatctc tttgtttttg tatttagtcc taaatcctta gcagcaggcg
    26641 cctctacctc tctttatggg ctatttgccg cgattattgt tcttcgctat gcaactcgca
    26701 atccttatat tcaacagcta gggcaatcct atctgacact ttttgtagtt aacattattg
    26761 gaagtgttct gattccagga atcagcctag caggccatat cggtggtgca gttggtggcg
    26821 catttctagc agttatcttt ccagttagag gagaaaaacg gatgtataat accagccaga
    26881 gattaggagc ggtagtcttg ttcgtaggac tcgccatttt gcttttctac aagggaatgg
    26941 gaatgtgacg aagttgaaag aaaatttgtg taatgaaaaa agataaggca ttttttgaac
    27001 cttatctttt tattttattt tattccttct cagtcaattc ttttccaagt tggatgaggt
    27061 aattcttcaa atcatctttg acttgtgggt gtttgagggc gtagtcgatg gatgttttca
    27121 tgaagccaaa cttgtctccg acatcgtaac gagccccatt gaactcacga gcaaatacac
    27181 gttgtgtttt attgagggtg tcgattgcat ctgtcagctg aatttcattt cctgcacctg
    27241 gagcttggtt ttcgaggatt tggaaaattt caggcgtgag gaggtagcgt ccgataatag
    27301 caaggtcgct aggagcgtcc tctggagctg gtttttcaac aaaggtttca acactgtaaa
    27361 gaccatcttt tccttcgcct tgcggagcaa taaccccgta agcagatact tcgtcatgag
    27421 ggactggcat gacagcgata gtagacgcgt gggtacgctc gtagtcatcc atgagttgtt
    27481 tggtaagtgg aacagccttt tcgtctgtga tatccatcaa gtcatcacca agcataacga
    27541 caaaaggttc atttccgacg aaagccttag cttgcaaaac agcatctccg agaccgcgtg
    27601 gatgagtttg gcggataaaa tgcagacgca tgtcagttgt tttatcaact agcttcaaaa
    27661 gatctgtttt ccctttttct ttgaggttat attccaattc gaagtttgaa tcaaagtggt
    27721 cctcaataga acgttttgat ttaccagtga caactagaat atcttcaata cctgatttga
    27781 gagcctcttc cacgataaac tggatagttg gtttgtctac gattggcaac atttctttgg
    27841 caagggcctt ggttgctggt aaaaatcgag ttcctagtcc agcagcaggg atgactgcct
    27901 ttctaacttt tgatgtcata aaaatccttt ctatagaggg ttaagaccac tcattttctg
    27961 ctttaaattc attgttcatg atgtcataaa tggcatcttt gatattggtt ccgtgataaa
    28021 taacttggta aatagcctgt gtaatgggca tatagactcc aagttcttgg gctagttcat
    28081 aggctgctcg agtcgttgaa attccttcga ttaccatgcc catattagct tctatatcag
    28141 ctagggattc tcctcgtccg agagcatctc cagctctcca gttacgagag tggatggaag
    28201 ttcccgttac gatcaaatct cccacaccag ataagccgct ataggtcaat ggactggccc
    28261 cgagtgctac ccctaggcgg gtgatttctg ctaaacctcg agcgatgatg gctgccttag
    28321 cattatcacc aaatccaaga ccatgtaaag ctccagcacc gacagcaata atatttttaa
    28381 gagcaccagc agtttcaacc ccgataacat ccgtattggt ataaagtcgg aagtagtgat
    28441 tactaaatag ctcctgaacg tattgagctg tttgtaaatc tttagaagca gcagtgatta
    28501 aagttaggtc acgcacaatg gtttcttccg catgactagg ccctgaaaca acgacaatat
    28561 cactgcggag ctgttcagga atttcttctt caaggatagt tgataatcgt ttatggctat
    28621 caggttctaa tccctttgaa gcgtgcatga tgatagcctt atggtccaag gtttgtgcaa
    28681 cttgttgggc aacaagtcgt gtcacttttg ttgggacaac aaacaaaatc gcatccacat
    28741 ctttcaatgt ttctgctaag tcggtgtagg caatgatatt ttcatctaga acgacatctt
    28801 taaagtagtg cttgttagta tgataggtat taatttcatt gatttgctcg ggaagatttc
    28861 cccaaatacg tacctcgtgt ccattgtcat ttaagacttg tgaaagggca gttccccaag
    28921 aaccaggccc caagacggcg acggtttgtt tttccatgat ttcctccttg atagagttct
    28981 ttccattatt ctatcatatt ctagaggatt ttgcacttgc attactgggg tatggtggaa
    29041 aaatacacaa agaccgagat gaagggataa actcttagtc tttagttaaa gtgagaagaa
    29101 atctgataga tagtttgatt cagtatatgc tataaataga ggtaaacaac tcaggaggtt
    29161 cttgtaggtt gcgagagttt ggcgaaaaaa ttaaaagatt acgtttggct aaaaaaatca
    29221 gtcgttcaga attttgtggt gatgagtctg aattaagtat ccgtcaatta attagaattg
    29281 aaaatggaga atccagacca acactaacaa agttaaaata tatcgctgaa cgtttggggg
    29341 ggggtgaaga ttacaagttg atgccaagtt atatagagtt ggataaggaa tacctagaat
    29401 tgaagtattt cttgatgagg actcctacat acgaagatga aactatcgcc caaaagaaag
    29461 agagtgtttt tgctaagatt tttgaagagt attatgatag gctacctgag gaagaaagat
    29521 ttatcatccc aaattattca tatctagcac tagcgaacta cacagttcaa aaattaccag
    29581 aaaagctagt tgaaatactg tccttctggt gattttgctt aatttctgat gttcgagaca
    29641 tgattgagga gatttgtctt gattttttcc taaaaaaggg cagactcctc ccttggtttg
    29701 atgctcgatt tttaaaataa ggtgacttat tctgttaggc atgttttccc ctgccctcta
    29761 gctctatatg gaacagggag tatcagattt atctggcaga ttgtatcaaa taaggcttga
    29821 aactgttttg aataggcgta tagacttgag aatttgagaa tatgtcgtct agcagtagag
    29881 acatattttc cggcaatatg aaggaagaga cgtcgaaaac gcttgatagt caaggacttt
    29941 acttcatcac cagctagctg ctttaaaaag aggtagagat tataagccag acagcccatc
    30001 atcatacgta cttcattctt aatcatggtc gaactatctg tcttatcccc aaagaatcct
    30061 gctttccttt ctttgataaa gttttcagca ttccctcgtt cacgatagaa ttgaaacagc
    30121 tcctcactat ctacactgct taaattgttc aaatgaactt ttttacaacc ataaaaaaga
    30181 gcacttaatc gtactcttct cttttaagga ttagttgaat taattcaaat ccactagttc
    30241 tcctgtttct aggtagacta ggcgttcaca aatgttagca atgtaatcag cgaagcgttc
    30301 cagatgtcct atgatataaa ggtattgagt tccattggga attgaggttt cttggtcttt
    30361 cataagtcca atgatttcct ttgataaggc ataataatat tggtcaatct gttcatcttt
    30421 ttgagcaata ctaatagctt ttgaggcttg gtgcaaagga aaggcaacca ataaatcggc
    30481 tagcatgctg agggataatt tacccatttg gtgtaactgt tcttcgtcag gggctagttg
    30541 attttctttt agttgcaaaa cagctttggc aatgcctgcc atatggtctc ccatacgttc
    30601 aaggtctgaa caagaagaca tgatgctaat cacaaatcga aggtcagaca cttgtggctg
    30661 ttgcaaggcc aacaaacggg cacaggtcaa ttcgatagcg ctttgacctt ggttgatagc
    30721 atgatcctta ttgataatta gctctgccat ctccttgtct ttggaggcta aggccagtaa
    30781 ggcttttgaa gctgtttcaa ggacaagttg ccctagtcct aaaaaggatt gttctaattc
    30841 atgcaattct aagtcaaatt gatttctcat aggtttttcc ttccttatcc gaaccgtcct
    30901 gaaatatagt cttctgtgcg ctgatctttt ggattggtaa acacgtcaac ggtatctcca
    30961 aattcgcaaa tttctcctgt taagaaaaaa gcagttttat ctgaaatacg tgaagcttgt
    31021 tgcatgttat gggtaacaat gataatcgta taatcctttt ttagttgctg aatgaggtct
    31081 tcaattttta aagtggagat agggtctaag gctgaagtcg gctcatccat taacagaaca
    31141 tcaggttcta ctgctaaagc tcgcgcaatg caaaggcgtt gctgctgacc gccagataag
    31201 gacatggcac tctttttaag atcatctttg acttcttccc aaatggctgc cccttttaaa
    31261 gatttctcca ctaaggcatc taattgtttt ttgtctcgaa taccatgtgt ccttggacca
    31321 taagccacgt tatcatagat agacatggca aagggattag gctgttgaaa aaccatccct
    31381 acacgcttgc gtagctgatt aaggttgaat ttgctactat aaatatcttg ctcatctaag
    31441 aggacttggc cttcaatatg gcaagaagga accaaatcgt tcatccggtt aagggttttt
    31501 agaaaagttg atttgccaca accagatggg cctatcaagg cagtaatctg tctttctggt
    31561 aattgaatcg aaatattttt taaggcttga aaatccccgt aaaataagtc taggtgtctg
    31621 actgaaaatg ttcccatact aggaagctcc tttcacaagt tttcgagata ataagcttga
    31681 tagagtattt atcattaaaa cagtaataat caaaatcaca ccggtagcat aggcttcatt
    31741 gacatgtagc ccctcacttg acagcatata catatgcagg gctagagaac gacctgaaga
    31801 catgagacta cttggcgtat tggtagaggt acctaatgta tacatgaggg cagctgtctc
    31861 accaacgata cggccaatag ctagtatcac tccagctaaa atacctggca tggcaactgg
    31921 tagaacaatt ctaaaaacag tccgtaactt acctgcccca agtccataac ttgcttgacg
    31981 catgctatca ctaacagata aaagggcttc ttctgttgag cgaataatga ctggcaacac
    32041 catgatgact gaggttaaga ttcctgataa cagagagtat tgaaaaccta agaagactac
    32101 aaagaagagc atgccaaaca gaccaaaaac aatggaagga atcccagaca aggtatctga
    32161 ggccaatcgc atgattttaa cacaaaggga atcttttttt gtatattcca caagataaaa
    32221 accagcaaaa atccctatgg gcaaggctaa aagaagagca ccaaagacca gaataacggt
    32281 ggaaataatc gctggcataa gggaaatgtt ctcagaagta taagtccaag aaaagaggga
    32341 tagacttaga tgaggtaagc ctttgatgag gataaaacca atgattaaaa agagagagcc
    32401 aaaggttaaa gctgaaaaac aataaacgag aagttttagc aggtatttac tcataagatg
    32461 attttccttt caagtaggca aagtaggcat taatcaagag aataaggaaa aagagaactg
    32521 ctgaggttgc aataagggct tccctatgct gacctgatgc gtaagccatt tccagaacaa
    32581 tattggttgt taaggttctg gttcctgaaa agagtccact tggaataatc ggctggttgc
    32641 ctgccaccaa aataactgcc atggtttcac ctactgcgcg accgattcct aaaataactg
    32701 ctgataaaat accagatctc gcagctggca agatgacact aaaaatactc cgttcatgac
    32761 tagctcctag agccaagcta ccagaataat acgttttggg aactgttcgg atagcagatt
    32821 ctgacaaact gataatggtt ggcaaaatca ttattcctaa tagtaacgaa gcggttagga
    32881 cactcatgcc atttcctaaa aaggttctaa tccaaggcac caataattgt aggccgaaaa
    32941 aaccataaac aatagatgga atggccgcca tcaagttgat agctgatttt aagaagccat
    33001 agacgggctt tggacaataa taaaccataa acaccgatgt caagatgcct gttggcaccc
    33061 caatcacaat cgctcctaag gtaattaata aggaaccaac gatcattggt aaaataccat
    33121 agcttgccgg aatgttcgtt ggcgaccaat cactgcctaa taaaaaacgg gcaaagccgt
    33181 agttagccat gaaaggtaag ccattactaa aaataaagaa acagattagc aaaatagcta
    33241 caacagctac tgttgcactc atgaaaaaaa ttgccctaaa aactgcttct ttgaaggctt
    33301 gttttgtcac atcttgtcct ttctagtgaa gaaagtaagg gagatacgac acctccctac
    33361 ttgccttctt tatcttattg tacgatgaaa cgtctgcatc tctttagaga tttatggagc
    33421 aaacatttta tttaatcttg tcccaggtgg ttaatttgcc actaaaaacg tctgcaagtt
    33481 cagccatact gacttggctt gccttattgt cattattgac cacaacagca ataccgtcta
    33541 aagcaatagc atcatgggtg agactcttac cttcttcagg agttaattcc ctagaaacca
    33601 taccaatatc agcggttttc tccttaacag cggtaatacc tgctgaagac ccattagagg
    33661 taatatcaat cgtaacttct ggattttctt ttttataagc ttctgctaat ttttccatta
    33721 aagaagatac tgaagtggaa cctacaacag acaacttgcc tgataagtgt tggcttgtat
    33781 attctgtggt ttcggtttta gcttcaataa atttattatc tgtgaccact tgttgacctt
    33841 gtttggagtg gataaagctg ataaaatctt gacctagctt ggaaagatta gaagaccaaa
    33901 caatgttgaa gggacgttga agagggtatt caccatctaa aactgtgtct cgactagcct
    33961 tgacaccatc aatctctaaa gccttgacag atttcgttaa agatcccaag gagatgtagc
    34021 cgatagcatt agcattccct tgaactgctg agagaacacc ttctgtacta ttttgaatca
    34081 cagctgtttt ggcagtgttg tcaatttttt tatcaccgtc ttttttgaga atccctgtga
    34141 tttctgtgaa ggcaccccgt gttccagagc cattttctcg tgaaatcacc tcaatcgttc
    34201 ctgaagctga ctgtttggaa gcagctgact gattgccaca ggcaacaagc ccaaatcctg
    34261 ataagccaat ggctgcaaga gtaagcattt ttttgaattt cataataatc acctttatct
    34321 ctatgtattt ttcttgtgta ggcttactac atttatagtc taacaagtct ttgtaaaggt
    34381 ttatccctga ttcatgtaaa gattgtgtaa agaatcaaaa aaagccactt ttgaaaaatg
    34441 gctgctccta aaaatagctt taaaaattat tagtcctgtg cgaaagattg gttaggaaga
    34501 aaaatcgtga agcaactgcc tctgccaagc tgactcgtca ccgtgacttg gccacctaat
    34561 aattgactga gttctttgac aatggcaagg ccaagaccag tgccaccagt ttgtctgctt
    34621 cgacctttat taactcggta aaaacgttca aaaatacgat cctgctctaa ttgactaata
    34681 ccaatccctg tatctgatac agaaatctta atgccttcgt tcaccttttg ggtcttgacc
    34741 tcaatttttc ccccttgttc agtgtaacgg atggcattgg ataaaagatt gagtaagatt
    34801 tgggaaagta attgactatc tgatacgagg gtgacatcat ctggcacctg cacctttagc
    34861 tgtaaatcct tcttcttgag ctgaggttgc aagctttgag tcaaatcctg tacaaattct
    34921 gccaaagaaa gggtcgtcca ttgtataggc atttgttgag ccttagataa ggtaagaaga
    34981 tgctcaacaa tatgctcaag acgcaagctt tctttgtaaa taatgtctag aaagtcatcc
    35041 ttgagcgctt cttcttcagc tgacatcccc ttaatggttt cagcaaagcc cttaatcgaa
    35101 gtaactggtg tcctcaattc atgggaggca tttgagacaa aggctaaatt taacttttca
    35161 taagttctaa tcgttgttaa atcatatagc aagacgagca cagcttccac agattgggtg
    35221 gggctaaaaa cgggaactgc tgtcacttct aaaatcaagt caccctcatg aaacccactt
    35281 acttcttgtt ttaaccttgt tttttgatca aaggcttggt gaactaaatt tcgaatatcc
    35341 atccgtttga ggtcatcaag tgaacttatg tcgccgtcca catcgggaaa ataatgaggc
    35401 agagagcgac tggataataa catctgacct tgagcggaaa ctaaaaacgt ccccatggtt
    35461 aggtgcgaca gaagaacctc cattgtttcg gctaggtcct tgtattgctg atcctgttgg
    35521 gagactttgg tttttagggc agacacatac tgagccaaag actttaagtc ttcttgccct
    35581 ttttctaaaa agtattcact actggtcaag agaggttggt gcaaggtctc aaaagcaact
    35641 tcccatttcc aaaggcaaaa gagccagtag ccaccttgtc ccaaagaaag ggctagaaga
    35701 aagagaccga tgcctttact gatccaagtt aatgccatcc ctgcaatcag aatgaggcta
    35761 acacttagat tgactagcca aaattgaagg tagcgtttca tctataactc cttgaactta
    35821 taaccataac cccgaatggt tcgaataaat tgaggggctt taggattgtc ttcaattttt
    35881 tccctcaact taccaatatg aacgtccacc aaacgtgttt cctgcccaaa gtcatacccc
    35941 cagatacgtt ccaaaagacg ctctctagtc agtgtcatgt tgggatgttt cataagatag
    36001 agcaagagtt caaattcttt tggggtcaaa ctcagtaact tattcgcctt gtagacttca
    36061 tgacgctcag ggtatacttt caaggtccca aatagccaag aatcgtcagc gatattatct
    36121 gaatcatctc cttcttgttc tcctttagtt cgcctgagga cagccttgac acgcgccagc
    36181 aattctctag ggctaaaagg cttggtcagg tagtcatcag cccctaattc caaggccaaa
    36241 accttatcaa attcatcact tttcgcagaa accatcataa ttggagtttt gacgcctttg
    36301 gctctcagcc gcttacaaac ttccatgcca tctaattgtg gtaacatgat atcaagcaag
    36361 ataaaatcaa agggttctgt ttctgccaaa gctaaggcct tccgtccatt tgttaccaat
    36421 tgagtagaaa agccttcctt acttaaatgg tagtcaagca atttcagaat gtgttcttca
    36481 tcatccacta ataagacttg ttttgtcatc tattatctcc tattggtaac attataacac
    36541 aattatcaga aatcctaaca ttgctaaatc agattaaatt tgcttatcaa gactagtatc
    36601 tggtcaaacg ctcaatcatc tccttgtgct ctggataggt cgccagtaga tctacccttt
    36661 caaataattc aaaatcctca aattcaaaac caggagcaac aagacaagaa accagagcat
    36721 aatccttatc aactgttgat ccccaaatag tgcccttagg aacacagtag tgaagttgtt
    36781 gccctttgga tatgtccagg cctaaagtga ctgcttcgta gtgaccatct gctgtaatca
    36841 tgtgaacagt aagtggggat cctgcatgaa aataccagat ttcatctgct gtcaatcggt
    36901 gaaaatgtga aggattcgtt tcttctaata agaaataaat actggtataa agcgcccttc
    36961 ccttaccagc aaggtttata gtgtctgaag ctttttttgt ttgtctaaaa tagccacctt
    37021 caacatgggg agctaactct agagttctta tcaagtcttc tttatccgtc ggagccaatg
    37081 ggttgaagta actcttgttc aaaggtggtg ttacgatttc aagaactcct ctcagctctg
    37141 aggacacgat aatgattgat gcgacggaag tacaaatcaa tcgccctaaa aaaagaatta
    37201 gcgaattatt ctgg
//
LOCUS       DAVBGY010000016        33375 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVBGY010000016 DAVBGY010000000
VERSION     DAVBGY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33375
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.016"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..327
                     /locus_tag="VQ620_000813"
     CDS             <1..327
                     /locus_tag="VQ620_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2763473.1"
                     /translation="KPQTEKPEEETPREEKPQSEKPESPKPTEEPEEESPEESEEPQV
                     ETEKVEEKMREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLLA
                     LLKESK"
     gene            749..979
                     /locus_tag="VQ620_000814"
     CDS             749..979
                     /locus_tag="VQ620_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HET2763474.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAIKC"
     gene            1383..2096
                     /locus_tag="VQ620_000815"
     CDS             1383..2096
                     /locus_tag="VQ620_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HET2763475.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            2154..2756
                     /locus_tag="VQ620_000816"
     CDS             2154..2756
                     /locus_tag="VQ620_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763476.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRFFSNFDDFLEKNYLKTIWQ
                     VSKETPFSEMDFNMFKNISEKIIFERGSKMLNDLKSNYKK"
     gene            2892..3545
                     /locus_tag="VQ620_000817"
     CDS             2892..3545
                     /locus_tag="VQ620_000817"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET2763477.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            3555..4019
                     /locus_tag="VQ620_000818"
     CDS             3555..4019
                     /locus_tag="VQ620_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2763478.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            4032..5300
                     /locus_tag="VQ620_000819"
     CDS             4032..5300
                     /locus_tag="VQ620_000819"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET2763479.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            5314..5574
                     /locus_tag="VQ620_000820"
     CDS             5314..5574
                     /locus_tag="VQ620_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763480.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            5585..6946
                     /gene="pdrM"
                     /locus_tag="VQ620_000821"
     CDS             5585..6946
                     /gene="pdrM"
                     /locus_tag="VQ620_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HET2763481.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            7100..8068
                     /locus_tag="VQ620_000822"
     CDS             7100..8068
                     /locus_tag="VQ620_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET2763482.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            8084..9076
                     /locus_tag="VQ620_000823"
     CDS             8084..9076
                     /locus_tag="VQ620_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET2763483.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDKNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYISEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            9294..10337
                     /locus_tag="VQ620_000824"
     CDS             9294..10337
                     /locus_tag="VQ620_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HET2763484.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            10401..12086
                     /gene="lpdA"
                     /locus_tag="VQ620_000825"
     CDS             10401..12086
                     /gene="lpdA"
                     /locus_tag="VQ620_000825"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET2763485.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEIIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMQSPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEIIGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            12150..13139
                     /locus_tag="VQ620_000826"
     CDS             12150..13139
                     /locus_tag="VQ620_000826"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HET2763486.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(13206..14276)
                     /gene="xerS"
                     /locus_tag="VQ620_000827"
     CDS             complement(13206..14276)
                     /gene="xerS"
                     /locus_tag="VQ620_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HET2763487.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
     gene            complement(14866..16416)
                     /locus_tag="VQ620_000828"
     CDS             complement(14866..16416)
                     /locus_tag="VQ620_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET2763488.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFIIIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(16432..17211)
                     /locus_tag="VQ620_000829"
     CDS             complement(16432..17211)
                     /locus_tag="VQ620_000829"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET2763489.1"
                     /translation="MATIKEIKELLVTVKELESPIFLDLEKDNRSGVQKEISKHKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQVVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(17198..18049)
                     /gene="ylqF"
                     /locus_tag="VQ620_000830"
     CDS             complement(17198..18049)
                     /gene="ylqF"
                     /locus_tag="VQ620_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HET2763490.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(18256..18432)
                     /locus_tag="VQ620_000831"
     CDS             complement(18256..18432)
                     /locus_tag="VQ620_000831"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2763491.1"
                     /translation="MQAGIPTDNEVSYALIYQQETLPATGSSTSALTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            complement(18484..24375)
                     /gene="iga"
                     /locus_tag="VQ620_000832"
     CDS             complement(18484..24375)
                     /gene="iga"
                     /locus_tag="VQ620_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin A1 protease"
                     /protein_id="HET2763492.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGSIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSENTGEVSSNPSDS
                     TTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEKKLEL
                     RNVSDIELYSQTNGTYRQHVSLDGIPENTDTYFVKVKSSAFKDVYIPVASITEEKRNG
                     QSVYKITAKAEKLQQELENKYVDNFTFYLDKKAKEENTNFTSFSNLVKAINQNPSGTY
                     HLAASLNANEVELGPDERSYIKDTFTGRLIGEKDGKNYAIYNLKKPLFENLSGATVEK
                     LSLKNVAISGKNDIGSLANEATNGTKIKQVHVDGVLAGERGVGGLLAKADQSSIAESS
                     FKGRIVNTYETTDAYNIGGLVGHLTGKNASIAKSKATVTISSNTNRSDQTVGGLAGLV
                     DQDAHIQNSYAEGDINNVKHFGKVAGVAGYLWDRTSGEEKHAGELTNVLSDVNVTNGN
                     AITGYHYTGMKVANTFSSKANRVFNVTLEKDEVVSKESFEERGTMLDASQIVSKKAEI
                     NPLTLPTVEPLSTSGKKDSDFSKIAHYQANRALVYKNIEKLLPFYNKSTIVKYGNLVK
                     ENSLLYQKELLSAVMMKDDQVITDIVSNKQTANKLLLHYNDHSSEKFDLKYQTDFANL
                     AEYNLGNTGLLYTPNQFLYDRDSIVKEVLPELQKLDYQSDAIRKTLGISPEVKLTELY
                     LEDQFSKTKQNLGDSLKKLLSADAGLASDNSVTRGYLVDKIKNNKEALLLGLTYLERW
                     YNFNYGQVNVKDLVMYHPDFFGKGNTSPLDTLIELGKSGFNNLLAKNNVDTYGISLAS
                     QHGTTDLFSTLEHYRKVFLPNTSNNDWFKSETKAYIVEEKSTIEEVKTKQGLAGTKYS
                     IGVYDRITSATWKYRNMVLPLLTLPERSVFVISTMSSLGFGAYDRYRSSDHKAGKALN
                     DFVEENARETAKRQRDHYDYWYRILDEQSREKLYRTILLYDAYKFGDDTTSGKATAEA
                     KFDSSNPAMKNFFGPVGNKVVHNQHGAYATGDGVYYMSYRMLDKDGAITYTHEMTHDS
                     DQDIYLGGYGRRNGLGPEFFAKGLLQAPDQPSDATITINSILKHSKSDSTEGSRLQVL
                     DPTERFQNAADLQNYVHNMFDLIYMMEYLEGQSIVNKLSVYQKMAALRKIENKYVKDP
                     ADGNEVYATNVVKELTEAEARNLNSFESLIDHNILSAREYQSGDYERNGYYTIKLFAP
                     IYSALSSEKGTPGDLMGRRIAYELLAAKGFKDGMVPYISNQYEEDAKQQGQTINLYGK
                     ERGLVTDELVLKKVFDGKYKTWAEFKTAMYQERVDQFGNLKQVTFKDPTKPWPSYGTK
                     TINNVDELQALMDQAVLKDAEGPRWSNYDPEIDSAVHKLKRAIFKAYLDQTNDFRSSI
                     FENKK"
     gene            complement(24584..25762)
                     /locus_tag="VQ620_000833"
     CDS             complement(24584..25762)
                     /locus_tag="VQ620_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763493.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCRNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLPLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWILNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            complement(25977..29627)
                     /gene="addA"
                     /locus_tag="VQ620_000834"
     CDS             complement(25977..29627)
                     /gene="addA"
                     /locus_tag="VQ620_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HET2763494.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEICSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FAKLGQLSDQITILDYQERYHQDTWKLAKTFQTFMSHFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMCLVIKEI
                     LKLHQEKGVTFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALVEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIDEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQIIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            complement(29624..32899)
                     /gene="rexB"
                     /locus_tag="VQ620_000835"
     CDS             complement(29624..32899)
                     /gene="rexB"
                     /locus_tag="VQ620_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HET2763495.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPISPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            complement(33099..33254)
                     /locus_tag="VQ620_000836"
                     /pseudo
     CDS             complement(33099..33254)
                     /locus_tag="VQ620_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
BASE COUNT         9532 a         6544 c         6872 g        10427 t
ORIGIN      
        1 aaacctcaga cagaaaaacc tgaggaagaa acccctcgag aagagaaacc gcaaagcgag
       61 aaaccagagt ctccaaaacc aacagaggaa ccagaagaag aatcaccaga ggaatcagaa
      121 gaacctcagg tcgagactga aaaggttgaa gaaaaaatga gagaggctga agatttactt
      181 ggaaaaatcc aggatccaat tatcaagtcc aatgccaaag agactctcac aggattaaaa
      241 aataatttac tatttggcac ccaggacaac aatactatta tggcagaagc tgaaaaacta
      301 ttggctttat taaaggagag taagtaaagg tagcagcatt ttctaactcc taaaaacagg
      361 ataggagaac gggaaaacga aaaatgagag cagaatgtga gttctagttc tcattttttt
      421 catgaaaatg tgcaaaatat agtagattga aactagaata gtatacctct acttctaaaa
      481 cattgttaga aatcgatttg actgtcctgt tcttatttca ttttactata tcttaacaga
      541 tagtgtaaat aaagataaac tatttactgg ctaattaatc agttaaacac tagttaagga
      601 gtaatgatga aaaaaagaac aatactatta ttgatggcca gtctgttagc tcttgtctta
      661 ggagcatgtg gtttcttgga catattgatc ctggatcatt ctcatcagga ttactcttta
      721 ctgctatttt agaaactggg gtggtttgat ggaaagtatt ggtattggtc ttgttatcgt
      781 ttcacattcc aaacacattg cagaaggtgt tgttgaactg attagtaaag tagctaaaga
      841 tgttccgatt acttatgtag gaggaaccga gggcggagga attggaacga gttttgatca
      901 agtagatagg gttgtttccg aaaatccagc agatacttta cttgcctttt ttgacctagg
      961 ttctgctata aaatgttaag taaatgattt caaaatcaga aagggattgc tttgggcagt
     1021 tcctttttaa tgtaaatgta aaaggagtag aatcatagtg tttctaaatt gtgaatcaat
     1081 caaaactgat tgtgatgggg ctattctagc tttagaatcc ttcaaaaatt aaaatttaag
     1141 gcaatcaatt acttggaaga gtatgaaagc atttagttta taggaattct aggcctagaa
     1201 ttacatatgt atttatgaag ataggggtgt tcgatagtta gtattgttct gttctgaaag
     1261 atttgagctg tcagttgtat agaaagtgtt cgaatttttt ttaagtgatt aaattagtta
     1321 attgtatgag gttctttatg atataatgtt cttaatgaat tttcagaaag gaaaacctca
     1381 aattgttcta caaatttcta ctcttcgacc tcgaccacac tcttcttgat tttgatgctg
     1441 ctgaggatgt ggctttgacc caacttctaa aagaagaagg agttgcggat attcaggctt
     1501 ataaagatta ttacgttcct atgaacaagg ctctctggaa agacttggag ctgaagaaaa
     1561 tcagtaaaca agagctggtt aacacgcgct tttctcgttt atttgctcat tttggacagg
     1621 aaaaagacgg tagttttctt gcccagcgtt accaatttta cctcgcccag cagggacaaa
     1681 cattatcggg cgctcatgat ctcttggaca gcctcattga gcgtgattat aacttgtatg
     1741 ctgcgacaaa tggcattact gccattcaga caggacgttt ggctcaatct ggtctagcac
     1801 cttatttcaa tcaagtcttt atctcagaac agttgcaaac tcaaaagccg gatgctctct
     1861 tttatgaaaa gattggccag caaattgctg gatttagtaa agaaaagacg ctgatgattg
     1921 gagattctct aaccgccgac attcaaggtg gcaataatgc ggggattgat actatctggt
     1981 ataatcctca tcacctcgaa aatcacacac aagcccagcc gacttacgaa gtctattctt
     2041 accaagactt gctggattgt ttagataaaa atattcttga aaagatcaca ttttaaagga
     2101 gacgagctaa tgactacaaa aaagctaata ttactattga agagtacatt gaaatgtctg
     2161 aagttgattt taatgaagct gttaattatg aatttacatc tgacacttgt caattagcaa
     2221 atagtattta tcaatctctt tttaagtttt ttgataagaa aaatttctct ggcgatttaa
     2281 tttttacttg gaaatctcca tcattagtca aagaagggga ttatattggg agaagggatt
     2341 cacaagtaga taatcttaga gtaataggaa atatatttcc gaattatctt actaatcgaa
     2401 aatatagcct caatatgaat cgtaatggct gtatgggaga ttttcctcat gacttttttg
     2461 atatatacct agatcatgta gcaaaatatg cctacgaaca aaaagttaat aatattaaag
     2521 agtattatcc tttaaaaaga gcgattttac accaagagaa tgcattgtat tttcgatttt
     2581 tttctaattt tgacgacttt ttagaaaaaa attatttaaa gactatatgg caagtttcta
     2641 aagaaactcc tttttctgaa atggatttta atatgtttaa aaatatttca gaaaagataa
     2701 tcttcgaaag aggtagtaaa atgcttaatg atttaaaaag taattataaa aaatagccag
     2761 tctttataaa ctgtattcac gtattcaaat cgtatatagt cgatttatta aatctttaga
     2821 taaactgtaa aaagtggttt ccatagccac tttttgctat aatagaggca gtaaaaatga
     2881 aggagtcggc tatggaacac tcgtcttggc atgctttgat taaggcgcaa ttacctgagg
     2941 gttatttcgg gaaaatcaat cagtttatgg agcaggtcta ttctcagggg attatttatc
     3001 cacccaagga aaaggttttt caggctctct tgacaacact gcttgaagaa gttaaggtgg
     3061 taattctagg gcaagacccc tatcacggac caggtcaagc gcagggcttg agtttttctg
     3121 tacctgactc tatcccagct ccaccatcct tgcaaaatat cttgaaagaa ttgtcagatg
     3181 atatcggagt taagaaatct catgatttga cagcttgggc tgagcaagga gtcttgcttc
     3241 ttaatgcttg tttgactgtt cctgctggac aggccaatgg tcatgctggt caaatctggg
     3301 agccttttac tgatgctgtg attcaggtgg tcaatcatct agatagacca gtcgtttttg
     3361 tactctgggg agcttatgca cgtaagaaga aggccttagt taccaatcct catcacttga
     3421 ttatcgaatc agcccatcca agtcctttgt cggtttatag aggattttgg ggttccaagc
     3481 ccttttccaa ggccaatgca ttcttaaaag agacaggaca agagccaatc gattggctta
     3541 gataaggaga aaatatgcct cagttagcga cgatttgcta cattgataat gggaaagaac
     3601 tgctcatgct ccatcgtaat aagaaaccca atgatgtcca tgaagggaaa tggattggtg
     3661 tgggtggtaa gctagagaga ggagagacgc cccaggaatg cgcggcgcgt gaaatccttg
     3721 aagaaacagg gctcaaagcc aagccagttc taaaaggtgt catcactttt cctgaattta
     3781 caccagattt agactggtac acctatgttt ttaaggtgac ggagtttgag ggcgacttga
     3841 ttgactgcaa tgaggggacg ctagaatggg ttccctatga cgaggttttg agcaagccga
     3901 cttgggaagg tgaccacacc tttgttgagt ggcttttaga ggataaaccc ttcttttcag
     3961 ccaagtttgt ttatgatggg gataaattgt tggataccca agttgatttc tatgaataaa
     4021 ggagaaagca gatgctacta atcaaaaatg gtcgtgtaat ggatcctaag tctggtttgg
     4081 atcaagtttg tgatgtctta gttcaagatg ggaaaattat caaaattgcg cctgagatca
     4141 cggaagaagg agcagaaacg attgatgcta ctggtcttgt ggttgctcct ggtttggtcg
     4201 atattcatgt gcatttccgt gaacctggtc aaacacataa agaagacatt catactggtg
     4261 ccctggcagc cgctgcaggt ggttttacta ctgtcgtcat gatggctaat accagtccaa
     4321 ccatttcaga cgtggagact ttgcaagcag ttctccagtc agctgccaaa gagaagatta
     4381 atgtcaagac agttgcgacc attactaaaa actttaatgg taaaaacttg actgacttta
     4441 aggcgctctt agaagctggt gcggttggtt tctctgatga cggtattccg cttgagagca
     4501 gtaagattgt caaggaagcc atggaggaag ccaaaaaact caataccttt atcagccttc
     4561 atgaggaaga tccaggtttg aacggtgttc ttggctttaa tgaaaatatt gctagagaac
     4621 atttccatat ctgcggtgct actggggtgg ctgagtacgc tatgatggcg cgtgatgtca
     4681 tgattgccta tgcaactaaa gcccatgttc acatccagca tttgtctaag gaagaaagtg
     4741 ttaaagtagt ggagtttgct caggggttag gtgcagaagt cacagcagaa gtagcgccac
     4801 agcatttctc taagacagaa gcacttcttt taacacaagg tagcaatgct aagatgaatc
     4861 caccgcttcg tttggaatca gaccgtcgtg ccgttatcga aggtctcaaa tcaggtgtca
     4921 tcacagttat tgcgactgac cacgcgcctc atcatgtaga tgaaaaaaat gttgaggata
     4981 ttaccaaagc gccatctggt atgactggct tagaaacatc cctgtctctc ggcttgacct
     5041 atttagtaga agctggtgag ttgagcttga tggaattact tgaaaaaatg acatacaacc
     5101 cagccaagct ttacaacttt gaagcaggtt acttggctga gaatggtcca gcagatatca
     5161 ctatttttga tgccaaggct gaccgccttg tggactccca ttttgcttcc aaagcagcta
     5221 attcaccatt catcggtgaa accttaaaag ggcaggttaa atataccatc tgtaagggac
     5281 aaatcgtcta tcaagcttga taggagttag aatatgaatt attttcgaaa acgtagggag
     5341 agacaagcga aatctaatag tggaatttac tgtcccgcag ctaatcgttc caaaattcaa
     5401 tatgaaacta aagaaaaagc tgaccatgcg gttcaatatg ataacggagg tttagttaga
     5461 agctattatt gtcgtacatg tgcgtgttgg catactactt caaaatctaa taaacctccg
     5521 ctaagtttga aaaactatgg tctgaaactc tttggtttag ataaatcaga atagaaagag
     5581 aagtatgtat aagacaaagt gtttacgaga gaagttagta ttatttttaa aaattttctt
     5641 cccaatcctg atctaccaat ttgccaatta ttctgcctct tttgttgata ctgcaatgac
     5701 aggtcaatac aacactatgg acttggctgg tgtatctatg gcaaccagta tctggaatcc
     5761 tttctttaca tttctaacag ggattgtgtc agccttggtg cctatcattg gtcaccatct
     5821 tggtcgaggc aaaaaggaag aagttgcgtc tgatttttac caatttattt atttggcctt
     5881 gggcctatct gtggtcttgc tggggatggt acttttcttg gcaccaacaa tcttgaatca
     5941 tattgggtta gaagcagcag tagcggcagt agcggttcgc tatctttggt ttttatctat
     6001 cgggattatc cccttgttgc tctttagcgt cattcgttcc ttgctggatt cgctgggctt
     6061 gaccaaactg tccatgtacc tcatgctttt gttactccct ctcaatagcg gatttaacta
     6121 tctcttgatt tacggtgcct ttggtgttcc agaactggga ggggctggtg ctggtttagg
     6181 aacatccttg gcctactggg tcttgcttgg gatttctgtt ctggttttat ttaaacagga
     6241 gaagctcaaa gccttacacc ttgagaaacg aattccactt aatatggata aaattaagga
     6301 aggagttcgt ttaggtctgc ctattggggg aactgtcttc gcggaagtgg ctatcttttc
     6361 agtggttggc ttgattatgg ctaagttttc gtccttgatt atagctagtc accagtcagc
     6421 tatgaacttt tcaagtctta tgtacgcctt tcctatgagt atctcatcgg ctatggctat
     6481 tgtcgtttcc tatgaagtgg gagccaagcg atttgatgat gcgaaaacct atattggtct
     6541 aggaagatgg actgccctca tttttgcggc cttcacctta accttccttt acatttttag
     6601 gggaaatgtg gccagtcttt atggtaacga cccaaaattt atcgatttga cagcgcgttt
     6661 tttaacttat agtcttttct tccagttagc agataccttt gcggcgccgc ttcagggaat
     6721 tttgcggggg tataaggata cagttattcc tttttacctt ggtttgcttg gttattgggg
     6781 cgtagcaatc cctgtggcta cgctatttga ttccctaaca gattttggag cttattctta
     6841 ctggatcggc ttgattatta gtttgattgt gagtggggcg ctctaccgtt ggcgtttaac
     6901 tgtgattatg aagagatttg aatctttagc aaaatccaaa cgctaaaaag tttgtgaaaa
     6961 aatattcttg ataagaaaat cccttgtttt cacggggttt tcttttttat tttgtgaaat
     7021 ttcttttaga aaaactttga cagtatttgc caaaaacggt aaaatagtaa ggtaagaaga
     7081 aagttagaaa ggcaagaaga tgtcaacttt agataaaaat cttttgctag aaatgttccg
     7141 taagatggaa gaaatccgtc gcatggactt aaaaattgca caattagtaa agaaagggaa
     7201 agtgccagga atgacgcact tttctgttgg tgaagaggca gctaacgtgg gggctatgtt
     7261 agctctcaat ccagatgatc tgattacctc aaaccaccgt ggacacgggc aagctattgc
     7321 taaagggatc gacctcaacg gaatgatggc tgaaatcctt ggtaaataca ctggaacctg
     7381 taaagggaaa ggtggatcta tgcatatcgc tgaccttgat gctgggaacc ttggtgccaa
     7441 tggtatcgta ggtggtggta tggggatcgc tgtcggtgca gccctcagtc agcaaatgca
     7501 aaataccggt aaaatcgttg tctgcttctt tggagatggt gcgaccaatg aaggtgtttt
     7561 ccacgaagca gtgaacatgg cttctatctg gaacctgcca gtcattttct attgcattaa
     7621 caacggttac ggtatctctg cggatatcaa gaaaatgacc aatatagaac atatccatca
     7681 acgtagcgcc gcttatggaa ttcctggaat gttcatcgaa gacggtaaca atgtcatcga
     7741 tgtctatgaa ggatttcaga aagctgtaga ccatgttcgc agtggcaatg gtccagtctt
     7801 gattgaaagt gtaacttatc gctggctcgg tcactcatca tctgaccctg gtaaatatcg
     7861 tacgcgtgaa gaagtggaat tgtggaaaca aaaagatcca atcgaaaacc tccgcaatta
     7921 ccttattgaa aataacattg caagtgccga agaattggaa gaaatccaag cgcaagtaaa
     7981 ggaagcagta gaagcttctg ttaaatttgc agaggaaagc ccattcccac cgcttgaatc
     8041 agcctttgaa gatatttacg cagactaagg agaaagagat aaaatggaaa caaaaacaat
     8101 gtccttccgt gacaccatta tccttgctat gtctgaggaa atgcgtcgcg atgaaaatgt
     8161 gttcttgatg ggagaagacg tcggtgtctt cggaggagac ttcggtactt ctgttggaat
     8221 gcttgaagaa tttggtccag aacgcgtccg tgactgtccg atttctgaag ctgccatctc
     8281 tggagcagca gcaggagcag ccatgacagg acttcgtcca atcgtcgata tgaccttcat
     8341 ggacttctct gttattgcca tggacaatat cgtcaaccaa gctgctaaaa cacgttacat
     8401 gtttggtggt aaaggtcagg ttccaatgac tgttcgttgt gcagctggta acggagttgg
     8461 ctctgccgcc cagcactcac aatctctaga gtcttggttt actcacattc caggacttaa
     8521 ggttgtggca cctggaacac ctgcggacat gaaaggcctg cttaagtctt ctatccgtga
     8581 taaaaaccca gttattatac ttgagtacaa gtcagaattt aaccaaaaag gggaagtgcc
     8641 agttgatcca gactacacaa ttccacttgg ggttggtgaa atcaaacgcc aaggaacgga
     8701 tgtaacagtt gttacttatg gaaaaatgct tcgccgtgtg gttcaagctg ctgaagaatt
     8761 agcagaagaa ggaatttcag ttgaaattgt tgacccacgt acccttgttc cgcttgataa
     8821 ggatatcatc ataaactcag tgaagaagac tggtaaggtt gttctggtca acgatgccca
     8881 caaaacaagt ggctatatca gtgaaatttc agctattatt tcagaatcag aagcatttga
     8941 ctatctagat gcaccaatcc gccgttgtgc aggagaagat gtgccgatgc cttatgcaca
     9001 aaacctagaa aatgcaatga ttccaacagt tgaaagtatc aaagatgcaa tccgaaaaac
     9061 ttataacaaa gaatagagtt acataagata agttatgtaa ataaaataaa gctgacgaga
     9121 aactttgtat cttttaggtg cagagctctt cgtcattaat atcttatatt cgagcacggg
     9181 ctaaaagctc ggaaaaaaga taaatctcct tggcttcatc gaagccatcg tcgatttcct
     9241 atttttcagt cgcttttgac gccctttgta tcataaatag aattggagag gtcatggctg
     9301 atgacaagct aagagcgact cctgcagcta gaaagttagc ggatgattta gggataaacc
     9361 tctacgacgt ttctggctca ggtgcaaacg gtcgtgtcca caaagaagac gtggaaactt
     9421 ataaagacac aaacgtggtt cgcatttcgc cacttgcaaa acgaattgcc ctcgaacata
     9481 acattgcttg gcaggaaatc caaggaaccg gtcatcgtgg taaaatcatg aagaaggatg
     9541 ttttggccct gcttcctgaa aatatcgaaa acgatagcat caaatctcct gctcagattg
     9601 aaaaagtgga agaagtccct gataacgtaa caccatatgg tgaaatcgag cgtattccaa
     9661 tgacaccaat gcgtaaggtt attgcccaac gcatggttga atcttacttg actgcgccaa
     9721 ccttcaccct caactatgaa gttgatatga ctgaaatgtt ggctcttcgt aagaaggttc
     9781 ttgagccaat catggaagca actgggaaga agactactgt aacagacctt ctttcgcttg
     9841 cagttgttaa gactcttatg aaacacccat acatcaacgc ttcattgaca gaagatggca
     9901 agactattat cactcacaac tatgtcaatc ttgccatggc agttgggatg gataatggat
     9961 tgatgacacc tgttgtttac aatgctgaga agatgagtct ttcagaacta gttgtagcct
    10021 ttaaggatgt tattggccgt accttggatg gtaaattggc tccaagtgag ttacaaaatt
    10081 caacattcac aatcagtaat ttgggaatgt ttggtgttca gtcctttggc ccgattatta
    10141 accaacccaa ctcagctatc cttggtgtca gttcgacaat cgagaagcca gttgtcgtca
    10201 atggtgaaat tgtgattcgc ccaatcatga gtttaggatt aaccattgac caccgtgtcg
    10261 tagatggtat ggctggtgct aagtttatga aggacttgaa agagttgatt gaaaccccaa
    10321 tctcaatgtt gatttaagag caagagtatt catttttaag atatagataa aggaaggaag
    10381 acatggcctt agaaataatt atgccaaaag ccggcgtgga tatgacagaa ggacaaatcg
    10441 tccaatggaa taaaaaagtc ggagaatttg taaaagaagg agaaatcctt ttggaaatca
    10501 tgactgataa agtcagcatg gaattggaag ccgaagaaga tgggtacttg attgccattc
    10561 tcaaaggaga tggtgaaact gtccctgtaa cggaaattat cggttacctt ggtgaagaaa
    10621 gggaaaacat cccaacagct ggagcagcat cgccagaagc tagccctgta cctgtagcaa
    10681 gtacctcaaa cgatgatggt aagagcgatg atgcttttga tatcgttgtg attggtggag
    10741 gtcctgctgg ttatgttgca gccattaaag ctgcccaact cggcggtaag gttgcccttg
    10801 ttgagaaatc tgaacttggt ggaacctgct taaaccgtgg atgtattcca accaagacct
    10861 accttcataa cgctgaaatt attgaaaata tcggtcatgc tgcaaatcgt ggtatcgtga
    10921 ttgaaaatcc taacttcact gttgatatgg aaaaactttt agaaactaaa tctaaagttg
    10981 ttaatactct tgttggtggt gttgcaggac ttcttcgtag ctacggagtt actgttcata
    11041 aaggaattgg tacaatcact aaagacaaga acgtcttggt aaatggttct gaattgcttg
    11101 aaaccaagaa aatcattctt gctggtggtt caaaagtcag caagatcaac gtccctggta
    11161 tgcaatctcc acttgtcatg actagtgatg acattcttga aatgaacgaa gtgccagaaa
    11221 gccttgttat catcggtggt ggagttgtcg gtatcgaact cggtcaggcc ttcatgacat
    11281 ttggttcaaa agtgactgtt atcgaaatga tggaccgtat agttccagct atggatgcgg
    11341 aagtttctaa gaaccttcgc ttgatccttg agcgtaaagg aatgaccatc ttgactggta
    11401 ctaaactgca agaaatcatt gaggaaaatg gtcaacttcg tatcaaggtt gaaggaaaag
    11461 acgatatcat cgcaagcaaa gctcttcttt caattggtcg tatgccagac cttgaaggta
    11521 ttggagaggt tgagtttgaa ttggatcgtg gttgtatcaa ggtcaacgaa tacatggaaa
    11581 cttcagttcc aggcatttat gcaccaggtg acatcaacgg tactaagatg ttggctcacg
    11641 cagctttccg catgggtgaa gtttccgctg aaaatgccct taaaggaaat catgcagttg
    11701 ccaaattgaa tttgactcct gcagccatct acactctccc tgaagtagca gcagtaggtt
    11761 tgacagaaga acaagcccgt gagaaatacg atgttgccat cggtaagttt aactttgctg
    11821 ctaacggtcg tgctattgca tctgacgcag ctcaaggttt cgtaaaagtt atcgctgata
    11881 agaaatacgg agaaatcatt ggtgtacaca ttattggtcc tgcagccgca gaattaataa
    11941 acgaagcatc aagcatcatc gaaatggaaa tcactgttga ggaaatgctg aagaccatcc
    12001 acggacaccc aacttactct gaagtgatgt atgaagcgtt tgcagatgtt ctaggaatgg
    12061 ccatccattc acctaagaaa aaataaaacg aagatagatt agactggaag gttatttcca
    12121 gtctctatca tataaagtaa agggatatca tgaaatacat tatcaatcat tcaaatgaca
    12181 ctgcttttaa tattgccttg gaagaatacg cctttaaaca ccttttggat gaggatcaaa
    12241 tcttcctact ttggattaac aagccatcta tcattgttgg tcgtcaccag aacactatcg
    12301 aagaaatcaa ccgtgattat gttcgcgaaa atggtattga ggtagttcgc cgtattagtg
    12361 gtggtggagc tgtttaccac gatttaaata acctcaacta cacgatcatc tccaaagaag
    12421 atgaaaataa ggcatttgac ttcaagagct tctcaactcc ggttatcaat accctggctc
    12481 aactcggggt taaagctgag ttcacaggtc gtaatgacct tgagattgat ggtaagaagt
    12541 tctgtggcaa tgcccaagcc tatatcaacg gccgtatcat gcaccacggt tgcttgctct
    12601 ttgacgttga tttgtcagtc ctcgcaaatg cccttaaggt ttcaaaagat aaatttgaat
    12661 caaaaggtgt gaaatccgtc cgtgcccgtg taaccaatat tatcaatgaa ttaccaaaaa
    12721 aaatcacagt cgaaaaattc cgtgatttac tcttggaata catgaaaaaa gagtacccag
    12781 agatgactga atacgtcttt tcagaagaag aattggccga aatcaatcac atcaaggata
    12841 ctaagtttgg aacttgggac tggaactatg gtaaatcacc tgaatttaac gtccgtcgtg
    12901 gaaccaaatt cactagtggt aaggttgaag tctttgctaa cgttactgaa tcaaaaatcc
    12961 aagacatcaa gatttatggt gacttctttg gtattgaaga cgttgctgca gtagaagatg
    13021 tccttcgtgg ggtgaaatac gaacgcgaag atgttcttaa ggcgctaaaa accattgata
    13081 tcacacgcta cttcgctggt attagccgtg aagaaatcgc tgaagcggta gttggataaa
    13141 tcaaaaaaag aagttggcca acttcttttt ttgatattta tttacataat ttaaaatacg
    13201 tgaaatcata aactatctag agcattcttt tgttcatcat taacaatatg ggtatagagg
    13261 tcagtgactt gtgtgctggc atgtcctagt tggtgactga ctaaaacttg tgatttagtc
    13321 gcatcataga gcctagttgc tagcgtatgg cgcagtttat ggggtgttac acgtacttta
    13381 aaatcctctg agtatttagc aaccattttc tcaacgctag aagcatcgat acgattagga
    13441 acacctctgt agagagttaa aaaaagggct gtatctgttt tttccgtttt atagcgttga
    13501 ttccgaatgg ccagataatt ctctaaataa ggtttagcaa aagcagcgac attgactgag
    13561 tcacgtttgc caccttttcg agtaacatca ataaccatca tttttagatt gagatctctt
    13621 agatctagat taacagcttc agataagcga acaccagatg ccaagagaag ggcaataatg
    13681 gctaaatctc gttctttatt tttgttgaat gatgagagag ctcgatttga aagctgttgt
    13741 gggtgctctt gatcgatata agttagaaaa ccttctgttt catcacctag aaagagtttt
    13801 tgcttgatat tttcagctct ggcagcaagg gtttctttct ttttcttggt tgaaactttt
    13861 ttcattacat tacgatagaa ataaggttcc ccctgatcgt tttcaacctc ctcggttaga
    13921 tacttgtaaa gactagaaag tgctgataag gttcgattga tagttgtctg tgaaacacct
    13981 tgttttgttg tattagcatt cagcaaggga cgttcacgta gataaaggat aaaggattcc
    14041 atgtctttct tagacatatt ttccaaaact gataaaggaa tatcggatat tttatcagcg
    14101 tttgaaatac cagactccaa aacccagctg aaaaatcggt catattcctt aaggtattcg
    14161 tacagggttg taaaactgta gggcacagcc agcttagatt ggtagtattc cagaacatac
    14221 cagggcatga gttgttttag tttgtcgatt cgttccagta aaatctcacg tttcatgtat
    14281 tttctccata agtaagtata ctagtagtat agcatagact tgtatatttt tcaagaaaat
    14341 tatagttttt cagaaagatg ttataggagt ttttcaaagt gataaaaaga cttagaccta
    14401 caaacaaaac agaataagga aaataataaa aatctcaccc actaaacttc tgttaatact
    14461 acataactga agagctatat aaggagctat gagatacaaa acacattgta taatagattg
    14521 aaactagaat agtacatctg gacttctaaa acattgctag aaattgattt gattgttctg
    14581 attgttttat cctatttcta tttcatttca ctatataatt ataattgcta ttgttttctt
    14641 tttcatgatt ttaatcttac tgtacagatt tgatacagta aactcctttc tagctttata
    14701 ctttatcatt tctaacttaa ttatagtctt atttttaata aagttcaatt atttatatgt
    14761 aaaattcctc atcagtaaaa aataaccgat ctcatttctg agaatcggtt ttctaaataa
    14821 atcaagctga gtatttacat aacataaatt atgtaaatac tcatactaca acaacaaatc
    14881 tttgactttt ccaatttcac tttttggaat aaccaggtga atcatatcac ccagatacat
    14941 tctggttgag ccgttaactg tttggctctt gccattatgg acttgagttg tgatgaggac
    15001 gttgtgtggt aagttgagtt catgaacttg tttcccagca attttatcag aaactggtat
    15061 ttcgataagt gtaacttctc cttcgctaga tacttcttct ggaagcattt tttccagcat
    15121 ggcttcatag actggcgtac ctttgagcaa atccatgata atataagaaa caagagtgac
    15181 aagaccaagt ggcatgaggt tgcgaatatc tcctaccatc tcagttacga ggatcatagc
    15241 ggttaaggga gcttttgata tggctccaaa atagccactc attcctagaa tgacaaatat
    15301 agggaattgc tcttgactga caagtccaag attgacacag ataacaccaa ctaaggcacc
    15361 aagcaaagaa ccaagagcta aaatggggag gaaaattcct cctggcagtc cacttccata
    15421 gctaatcata ctccaaataa agcggatcaa aaagtaagct aataaaactt ggaaactaaa
    15481 attttgttca gttaaagaaa ggacaagctg atttccgcca ccaatgattt gaggtaagaa
    15541 gattccgact ggtatgataa tgataaaagc caagatggga taataagccc tatccaaatg
    15601 gattttttga ccaatcaagt cataaactct tccaacgttt aatacagctt tctcatagag
    15661 aaaacctgaa aatccaagga aaattcccat gacgagatat atccaatact ggtctagggt
    15721 catgggagga atgttatctg gcatatccaa tactggtgtc aaaccgaaca tgagtagaga
    15781 cacaaagttt gctacgatgc tggctgctag agttgagacc cagaaaaagc gcgaaaaatg
    15841 gtgatagact tcttctacaa caaagagaag tgctgcaata ggagcattaa aggctgcggc
    15901 taaacctgct gcagctccac tggcaatcaa ggaacgttcc tctactggac tggatttgag
    15961 ccacttggca attcctttac caccaactgc tccaagttga atgctgggtc cctctcgacc
    16021 cagcatgagt ccactggcaa tagcaagaat acctagcaca tattttttcc aaagaatgcc
    16081 ccaccagttg agggacatga ggcctttcag ttcggcttcg acttgaggaa ttcctgagcc
    16141 tttaatatct ttttctgacc gtgttagttt ggcactgagc caacagatga gtatataaaa
    16201 caaaaccagt acaaaaagat tgcgcactaa gtacccttga tcttgataaa ctccttgtat
    16261 caggtggaag cccttttcaa ttaagaaacg aaaggatccg acgataattc caacgatgag
    16321 gccgacaatg attcctcgac caacttggga tagtatagtg ctggatgcaa aggcgaattc
    16381 tttcttagaa cggactattt ccgactgttc ctccatggta tttcctttca attaactttc
    16441 ttttttacct aaaaccagtg atttaacggg ttcaaagctg gttcggtgaa ttggggtaac
    16501 tcctagtttt gtgaggcctt ccagatgttt agctgtgcca tatcctgcat tagtagcgaa
    16561 atcatagcca gggaactgct gatcgtattc cttcatcaat tcatcacgtg ttaccttggc
    16621 tactatagat gctgctgcga tagagaggga gttggcatct cctttgataa tggaggtttg
    16681 tgaaatgggc aagtccagtt tcatggcatc aatcaaaagg tgctctggtt gagggctgag
    16741 ctgggagatt gcttcttgca tggctagttt ggttgcttca tagatattga cttggtcgat
    16801 gacctgatta tctatgatac caattccaat cgacaaggct tggtcttgaa cgacttggaa
    16861 aatctccaga tgtttctttt taggaatttt cttgctgtcg ttgagacctt taatcttaca
    16921 atttttaggt aaaataacgg ccgcagcgac tacaggacca gcaagaggac cacgaccaac
    16981 ctcatcaata cctgcaatta aggtcaatcc ttgcttataa agttcttttt cataagaaag
    17041 catggattcc aagcgcaaat tttcatctaa ttcagcttga atggctcttt tatgcttgct
    17101 gatttccttt tgaactcctg agcgattatc cttttcaaga tctaaaaaaa tagggctttc
    17161 taactccttg actgtcacaa ggagttcttt gatttcttta atcgttgcca tcgaggtctt
    17221 ccaatgtatc taaggtatag ttaccgagtt tgccatcacg gacttccttc acgaagagac
    17281 tgtaaaaacg gtcatagtca tcacggaaac cgagggcgcg ggtcatatcc ataataatca
    17341 caggcgcttc ttcttcaatt ttcatttgtt tgaagcgttc agccagcttt tctggataat
    17401 gttctttgaa ataattgata ccaaaaatgg taacctcatc cataggaagc aactgatcct
    17461 tgatagctcc agtcaatgcc aacttaagtg caacagtttc atcctcaaac ttaggccaga
    17521 gaatccccgg tgtatccaag atttccagat ctttattggt tttaagccat tgttgacctt
    17581 ttgtgacccc tggcttgttt ccaacaacag caatcttttt accagccaaa cggttcatca
    17641 gagttgattt accagcgttt ggaatcccaa taatcatggt acgcaaggtt tcaatctgaa
    17701 tcccacgttc tttctggcga gcaatcttat cagccatgag cttcttggcc gcatctgtta
    17761 caacttttac agtcacttgc tctttggagt tgatagctag cgtctggatt ccttgtgatt
    17821 caaaatactg acgccattcc ttggtcattg ctggatcagc caagtcggcc ttgtttaaaa
    17881 tcaagagttt tggtttatca ccaacaatct tggttaacat aggattttga ctagatagag
    17941 gtaagcgtgc atcaactaaa atcgtcacaa aatcaacaaa ttttaaattc tcctgcacct
    18001 gtcgacgagc tttagacatg tgaccaggaa accattgaat agtagccatt gagttttttc
    18061 ctttcatagc aaggatttag atcccctatt ttattttact attgtttgaa catagagagt
    18121 tttcaaatga atgatgtcca aacagttctt gttttatgac atagaaatct actttatatt
    18181 atatcatgcc ttctttattt tgtccgatga tagcctttta ccctatatag aaaaaagcca
    18241 tcaaatgatg acttttcaga gggagcaaaa aactgttccc gtcatattat gaaccaaaag
    18301 aactaaacta ccaacagcta ataggcctaa agctgtaagt gcagaagttg atgaacctgt
    18361 tgcaggaaga gtttcctgct gataaataag agcataactt acttcattat cagtaggaat
    18421 tcctgcttga atcttggttt gttcttcatc acctttttaa actttatttc ctaatagtaa
    18481 acactatttt ttattctcaa aaattgaact tctaaaatca tttgtttggt caagataggc
    18541 tttaaagatt gctctcttca acttatgaac ggcactgtcg atttctggat cataattact
    18601 ccaacgtgga ccttcagcat ccttgagaac agcttggtcc atgagggctt gcaattcatc
    18661 cacattattg atagtctttg tgccatagct tggccatggt tttgtcggat ctttaaaggt
    18721 cacctgcttc aagtttccaa actgatccac tcgttcttgg tacatagctg tcttaaattc
    18781 tgcccaagtt ttatacttac cgtcaaatac ctttttcaaa acaagctcat cggtcaccaa
    18841 gccccgttct ttaccataaa gattgatagt ttgcccttgt tgtttagcat cttcttcgta
    18901 ttggtttgag atataaggta ccattccatc cttaaagcct ttggcagcca aaagttcgta
    18961 cgcaatccta cgtcccataa ggtcccctgg tgtgcctttc tcactgctga gagctgaata
    19021 gattggggca aagagtttga tggtatagta gccatttcgt tcatagtcgc cagactggta
    19081 ctcacgagct gataagatgt tgtggtcaat caaactttca aaactattca ggtttcgggc
    19141 ctctgcttct gtcaattctt tgactacgtt agtggcataa acctcatttc catctgctgg
    19201 atctttcaca tacttgttct caatttttct gagagccgcc attttctggt aaacacttag
    19261 tttattaacg attgactgcc cttcgagata ttccatcatg tagataaggt caaacatgtt
    19321 atggacatag ttctgaagat ctgctgcgtt ttggaatctc tctgtcggat ccaagacttg
    19381 aagacgggat ccctctgtac tatctgattt tgagtgtttc aagatagaat taatggtaat
    19441 ggttgcgtca cttggttggt caggggcttg caataagcct tttgcgaaga actctggtcc
    19501 caagccattt cttcgaccat agccaccaag gtaaatatcc tgatccgaat catgggtcat
    19561 ttcatgggta taagtaatgg ctccatcctt gtccagcatg cggtaagaca tatagtagac
    19621 gccgtcccct gtagcgtagg ctccatgctg gttgtgtact accttattgc caactggtcc
    19681 aaagaagttc ttcatagctg gattggagct atcaaactta gcctccgctg tagcttttcc
    19741 tgatgttgtg tcatccccaa acttataggc atcataaagg agaatcgtac gatagagttt
    19801 ttcgcgtgac tgttcatcta aaatacgata ccaataatcg tagtgatctc gctgacgttt
    19861 ggctgtttca cgcgcatttt cttcaacaaa atcattgaga gccttgcccg ctttatggtc
    19921 actactgcgg tagcgatcat aagctccaaa tcctagacta gacatggtcg agatgacaaa
    19981 tacggatctc tctggcaagg tcaggagagg caagaccata ttgcggtatt tccatgtggc
    20041 actcgtgata cgatcataaa caccgataga atacttggtg ccagctaacc cttgcttcgt
    20101 tttcacctct tcgatagtgg atttttcttc gacaatgtaa gccttagtct ctgatttaaa
    20161 ccagtcatta ttgcttgtat ttggtaaaaa gacttttcgg taatgttcca gcgtgctaaa
    20221 caaatctgtc gttccatgtt gactggcaag actgatacca taagtatcga cattattctt
    20281 agctagaaga ttgttaaagc cagatttacc taactcaatc agagtatcta gtggggaagt
    20341 atttccttta ccaaagaagt ctggatgata cataactagg tctttgacat tcacttgacc
    20401 atagttaaag ttataccaac gttctaaata agttaaaccg agtagcaagg cttccttatt
    20461 attcttgatt ttatctacaa gatagcctct ggtgactgag ttatcgctag ctagaccggc
    20521 atctgctgac aaaagttttt tcaagctgtc tcccagattt tgttttgttt tggagaactg
    20581 gtcttctaaa tagagctcgg ttaacttaac ttctggagaa ataccaagtg tctttctgat
    20641 agcatctgac tggtaatcaa gcttctgcaa ctcaggcaag acttccttaa caatagagtc
    20701 tcgatcatat aagaattggt taggagtgta gagaagtccc gtattaccta gattatattc
    20761 tgctagattg gcaaaatcag tctggtactt gagatcaaat ttctcagatg aatggtcatt
    20821 atagtgaagt aagagtttgt ttgcagtctg tttgttggaa acaatatctg tgattacttg
    20881 gtcatccttc atcataactg cagataagag ttctttttgg tataagagac tgttctcctt
    20941 aaccaggttt ccgtatttga cgatggttga cttattataa aaaggtaaca atttttcaat
    21001 gttcttataa accaaagcac ggttagcttg ataatgggct atcttagaaa aatcactgtc
    21061 tttcttgcca cttgttgaga ggggttccac cgttggtaga gtgagaggat ttatttctgc
    21121 ttttttgctt acaatttgag aagcatctag cattgttcct ctttcttcaa aggattcctt
    21181 gctgacgacc tcatccttct ctaaagtgac attgaaaact ctattagcct tactactaaa
    21241 tgtattagct accttcattc ctgtatagtg gtatccagtg atggcatttc cgtttgttac
    21301 attgacatcg ctaagaacat tggtcaattc accagcgtgt ttttcctcac cagaagttcg
    21361 atcccacaaa tatcctgcca caccagcgac tttaccaaag tgcttgacat tattaatatc
    21421 accttccgca taactgttct gtatatgcgc atcttggtct actagacctg caagaccacc
    21481 gacagtctga tctgacctat tggtgtttga cgaaatggtt actgtcgctt tggatttagc
    21541 aatagacgca ttttttcctg ttaaatgacc gaccagaccg ccaatattgt aggcatcagt
    21601 cgtttcatag gtattgacaa ttcttccctt gaaactgctc tctgcgatgc ttgattggtc
    21661 tgccttagcc aacaaaccac cgacaccacg ttctccagcc agaacaccat caacatgaac
    21721 ttgtttaatc tttgtgccat tcgtagcttc atttgctagt gaaccaatat catttttccc
    21781 tgaaatagca acatttttta gactcagttt ttctactgta gcaccactca agttttcaaa
    21841 cagaggtttt ttcaaattat agatagcata attcttgcca tctttttcac cgattaaacg
    21901 accagtaaag gtgtccttga tataggatct ttcatcagga ccaagctcca cttcgttagc
    21961 attcaggctg gccgctaaat gataggttcc agagggattt tggtttatag ctttgaccag
    22021 gttgctaaag gaagtaaagt ttgtattttc ctctttagcc ttcttatcga ggtagaaggt
    22081 gaaattgtcg acatatttat tttctagttc ttgctggagt ttctcagctt tggctgtgat
    22141 tttataaact gactgcccat ttcttttctc ttcagttatt gaagctactg gtatatagac
    22201 atctttaaat gctgaagatt ttaccttgac aaagtaagta tccgtattct ctggaattcc
    22261 atccaatgaa acatgttgtc tgtaggttcc attcgtctga ctatacaact caatatcaga
    22321 aacatttctt aattcgagtt ttttctctgg ttctgtttgt ccattctctg atgttgtatt
    22381 acctgaattt tctggttttg ttgcagttcc gtttttttct ggttgatttg attcttcaac
    22441 tggtggtttt gaatcactag gtttattgga tacttctcca gtattttcgt tagctatttt
    22501 cccagagttt gtttgtgttt cttctgcagg ttgaactggt ttttctgttt cttgatttga
    22561 ggtaccttct actgtgcctt catttggatt tactggaact tcttctacag ttttttctga
    22621 attttcattt ttagagtcat tatgttctgg tttatttgat tctccaactg aggttgtcga
    22681 atcactagga ttactggaca cttccccagt attttcgcta gatgtatctg gtgatacttt
    22741 ctctgaattc gttgttgatt cttctgcagg ttgaactgga ttttctgctt cttgaattga
    22801 ggttccttct gtagtacctt catttggatt tactggtgtt tcttctgttg gttttactgg
    22861 aacttcttca gttttttctg gaccttgttc tttggtcttc tcaaccggag tttcaggttt
    22921 tacttgctca atattaccct tatattctgg caatggggct acctgctctg gttcaccttt
    22981 atcacttacc acagtatctg gcgactctgg ttgaacctca gtctcacctt tgtcggtcac
    23041 aactgcttcg ggtaatgtcg gttgaacttc tggttcgcct ttatcactta ctacagcttc
    23101 gggcaactca ggctgaattg cgggttcaac aatagatcca gactgtacgt ccttatgttc
    23161 tacaccagtc tcaggttgtt cctttataac ttgagttttt ttagtacctt tttcgactat
    23221 tcttggacta ggcgcagtcg ttgaagttga aacaatttct cgcgaaactt cttccttgtt
    23281 tacagagaat attctgacga tttcaacttt cttacctaat ttaccttctt gttttactct
    23341 tacagttcct tcagctaaat caggattttc ttgaatttct tcttgaaaat ctatttttgt
    23401 ctctatagtt tcctcacgat ataagagttc aggtttgttc aattgacctg ataaaacttc
    23461 atcctgtgga tttaatgtat ttaccccagt cttttctttt ggagaaatct tctcctcttt
    23521 cttcgtttct agattcttat gttcggctaa ttgttcttga gaatctgaag attgtttctc
    23581 ttcttttctt ggattgatta attcagtaga gaaaggtttt tcaactactt gaacttctgt
    23641 cggcttagtt gaagaaacag gtgtttgttc ctgaatagct tgtactgttg atggatggtc
    23701 tacaaaattt ggtgtaacat tataatccac cttttgttgt tttgtaggag tggctgctga
    23761 tttttcttga ttacttactt caaaatcaga agtcgttttt ccctctttga tatatccaat
    23821 ataagtgtaa cctgaaatct ctttaggaag aggtaatttt tctccagagg tcaattcata
    23881 gtccgtattg taatttagca aaagatgatt ttctaaagca tggactgaaa ctaagacacc
    23941 atttcccatt cccgcaacca atactaaatg taataccgtt ttattcttaa cctttttctt
    24001 ggaaacagca aaaattaaaa ttcccagagc agctaagcta gcaccagcaa ctagggcttg
    24061 cctctcattc ttgcttccag tatttggtaa ttccgccagt tgattttgag aatttaactt
    24121 ataaacaaga taataagttt catcatcatt ctccacgtat gtcggaatat catagacaag
    24181 ctgcttcttt tcttctgatg atagctctga atctgccaca tatttatagt gaactcccgc
    24241 agtttcttga gcatccacag atgaactagc taatacagac ataaaaaata aacttgaaat
    24301 cgttgcagat acaagtccta ctgataattt tctaaatgaa aaacgctctt gtttttcacc
    24361 aaaatacttt tccattattc ctccttgaaa taaaatttat atatgttaca aagaccttta
    24421 ttatattagt gtattatcta ttatctatag aaaaggcagt ataccttaat tatactctta
    24481 atttacaaaa aagtcttaaa attgagatgc gctttcatac tttgttttat attatttggg
    24541 ggtacaataa cacctaccat gaaatttaca cggtaggtgt tactcatatc actaatcgtt
    24601 ctaaaaatgg tttgaggcag ttgaggagaa ttccttctat ccagcttcct tgtgctgatg
    24661 agcgatggtc ttcctgcagg ctttttttta gaaaatctcg gacttgttct ggtgcgattt
    24721 caaattcaaa ggctttcatt ttatagaaaa agtcgatgag atgatctgac aggtattcag
    24781 ttgaaaaggg tacttcacca ctttttcgat attctaataa gagtctagaa aatcgagctt
    24841 tttcttcagg aagctcacga aaataggaat tgaggatcca agtctgcttc tgttttcttt
    24901 caattggatc ctgactggca attcgttggt ctttttccag ctctttttgg tattgtttgg
    24961 ccttgatagc tcgttctgct ctatttttac caaaaagaat tttttcccac ttgcgttctt
    25021 cttgagtcag ggtctctgta aagccaaagt aatcttgata agcacgctct gcgggtccca
    25081 tggctagaac cagattgtct gcatattgct tggcgatttt atccctcttc ttgcgttctt
    25141 tctctgcctg gatacggagt tcttgttcgt agtcaatttt ctccttgcct agcttgacaa
    25201 ggtagagttg gtcatccgat ttcccaagta aaaagggttt gatacacttt tcaaggactt
    25261 cttccatccg agcctttttc tttggttccg ccttggtcca acttcctccc tgaaagactt
    25321 ctaggaaaag ctggtagtct ctctcaggcg caaattgatt gccacgattg ggtttgaaaa
    25381 cacctttttc ccagagccat tttagaagtc gctcgtcaaa gtcactttta ttgaccttga
    25441 ttttttcctt tttctgagct tttctggtta gattttcaac ctttctgagc agtttttctt
    25501 cctcttccaa ttgctggtca aggggcaatc gatgaaaatg acgaacacag tcgctaccaa
    25561 ttggaaagag gcgttggcct gtgacaccgt taaagagttc ataagcgtat ttgatggcat
    25621 ttcgacagac acaattgcta cggccaatac cgttaaaaat caaggaaact tcattccatt
    25681 ccttggtagc ttgttcccaa gtatccgctt tcgaagcctg taaaactgca tcgtgcaggg
    25741 attttctaac tggaagtgtc atgaggtctc ctttctaata ctcaataaaa atcaaagagc
    25801 aaactagaaa gctagccgca gtcagctcaa aacactgttt tgaggttgta gatagaactg
    25861 acgaagtcag ctcaaaacac tgttttgagg ttgtggatag aactgacgaa gtcagtaacc
    25921 atatatacag caaggcgaag ctgacgtggt ttgaagagat tttcgaagag tataagttat
    25981 acttttacaa cttgaacctc gtctttaccg agtaaaatca agtatttttc aatattttca
    26041 atcgaatagg ctcgtgataa agcctcttcg tatagagcta actgaccacg atagcggtct
    26101 acgagttgac ttggttcatc atagcggtct gtcttgtagt cgaacagaac aattttgttt
    26161 tcgtaaagca gatagccatc aaggatacca cggacaacaa agtcttcctg actcttttgg
    26221 tctcgtttga gcatggagaa aggttgctcg cgatagagat ggtcggtatt agcaagaatt
    26281 tcctgaccga gtgctgtgtc aaagaaagca agaattttat caagattgat cttgtctctg
    26341 acagcttggc tagtttgaac ttgtttgagt gtttctgtta agctagcaag ggttagttgc
    26401 tggctgaggt caattatctg catgagttcg tgagtagcac taccaatctc agctccagtt
    26461 accttttctt tggttgaaaa atctggcaaa tcgaagctga ttttcttgcc tactgactga
    26521 ccttgaccag caatctcgac accttccata tccataactg gttcgtagaa tttcttgatt
    26581 tgacttgggg tttgaacact aggaagttca atagctgcgc ggtgaagagt attataaact
    26641 tccacctcct tcagcatttc cagagcttct ttgatggtat ctgactgacg attgtctgct
    26701 tgggagctat cttggagagg actcttggtt tccaactctc cgatagcttc tctggtcaac
    26761 tgatcttcgt caataaaacg ataactaaag ttgagcttgt ccttagtaaa cactttactg
    26821 atagcccaaa gccaatcttg gaaattccgt gcttgcagtc tagtattgct atttagtttc
    26881 ccatttttgg ctgctgggta ttccttggat tccagctttt cacgagaacc cttgccgaca
    26941 agatagagct ttttctcagc ccgcgtcata gcaacataca gcaaacgcat ctgctcagaa
    27001 tagcttgcta gctgtaattc ctcttcgttc tgcctatagg tcagactagg aatggagagt
    27061 ttgatggttt taggatagtg gtcttctact gcccctgtct ccatcttggc aatatatttg
    27121 acaccaagac cattctgacg actgagaatg acttctgaca tagagtcttg cttgttgaaa
    27181 tcttgatcca tattgaggat aaagacgtaa ggaaactcca gccctttact cttgtggatg
    27241 gtcatgagct ctactgcatc ttttggcggt gcgacggcca cgcttgccaa atcgtgctgg
    27301 gcttctaaga cttggtcaat catacgaata aaacgcgaca aacctttgaa attgctcttt
    27361 tcaaattgat cagcacgcag tgctagggca tagagattgg cctgcctagc aggaccattc
    27421 ggcaaagccc caacatagtc ataataaaaa cggtcgttgt aaatcttcca aatcaagtca
    27481 tagagagagt gggttttggc atacaagcgc caagaagcta ggatatccat gaattgcttt
    27541 agtttttcaa ctagagctgt gtgaatcaag cctttttgac tacttgccat tttttgtgca
    27601 ttgaccagtt tctcatagag attttcgtgg actttatcct ctgctttctg aagggacaaa
    27661 cgtgctagct catcctcatc aaaaccaaac attggagact tcataagggc aaccaaggcg
    27721 tagtcttgca ggggattgtg aatgacacga agagtgtcta gcatgacttg cacttctagg
    27781 gattggagat aattgttttg ctctccgtca gttttgacag gaatcccgta ctcagacagg
    27841 gcgaggagaa tctggtcatt acgactgcgg ctggaggtca gaagggcaat ttccttaaag
    27901 gtaacacctt tttcttgatg aagtttcaga atctccttga taactaagca catttcgcct
    27961 gttagtttcg tttctgtttg actctcttct tcctcacctg tatcgtcctt gtcgtagagg
    28021 agaaattctg ccttgttgtc tggattggga gtcagtttgg tattggcaaa aacaagctgg
    28081 tgcttgttat catagttgat ttcaccgacc tcttggtcca tgagacgttc aaagacatca
    28141 ttggttgctg acagcacttc tgaactacta cggaaatttt ccttgaggag aatgagcttg
    28201 ccttcttggg gattttgcgc atagcgttgg aatttctcat tgaaaatctg cgggtctgcc
    28261 tgacggaaac gatagatgga ttgcttgata tctcccacca taaagcgatt gtggccatta
    28321 gacaacaatt ccagcatccg ttcttgaata tggttggtat cctgatactc atcgaccatg
    28381 acttcatgga agcgctcctg ataagactca cgaacttgtg ggaaattctc taaaatctca
    28441 atggtgtaat ggctgatatc agcgaattca aaggcatttt cctggcgttt gcgctgacga
    28501 taagcctcca caaaatgact cataaaggtt tggaaggttt tagctagttt ccatgtgtct
    28561 tgatgataac gttcttgata gtcgagaatg gttatctggt ctgataattg tcctagttta
    28621 gcaaactggg tctttctctc ttcgttgtag gcatcagcca ggggcttcaa atcagcctta
    28681 cgactggcat tagtcagagc tcgaccgttt ttctccttag agatggcgac aacacgcgca
    28741 agcactgcct gataagcctg actatcggac tcctgattta gggagcaaat ttcatccaga
    28801 attaactgaa cattttctaa ataggcagcc tttgcaaact ccttggcatc gttatccaga
    28861 tggtaacgga aaaagctttc caaatcccaa agggcttgtt tgatttgctc agtcagtttt
    28921 tctttttcac tggtaaaatc agctttctca aatcctttga ggaaagattc actcagccac
    28981 ttttgaggat tactggtgga ttggaggaag tcatagattt tatagacctg ctggcgcaga
    29041 ccccgttcgt ccttgccacg cccagcaaag tttttcagca aatgactaaa ggtctctttc
    29101 tgtttacctt ggtaatgcgc ttcaaagacc tcatgaaaga cttcgttttc gagaagaagt
    29161 tgctcgcttt ggttttgtaa aatacggaaa ttaggtgcaa tatcaagcag ataaccatgt
    29221 ttgccaagga atttttgtgt gaaagagtcc atggttccga tggcagcgtt tggtaggtct
    29281 gccaactggc gtcccaagtg ttgtttgagg tcgacatcat ctgtttcttg gattttcttg
    29341 ctgatttttt tctctaaacg ttctttaagt tcagttgcag ccttgacggt aaaggttgag
    29401 ataaagagtt gagaaatttc gacaccacgc gccaattggt ccagaatgcg ctctgccatg
    29461 acaaaggtct ttccagaacc agccgatgct gagaccagga tattctgacc agaagtgtag
    29521 atagcttcga tttgctcggc agttttcttt tgttccttgc tcgaatttgc ttctgcttct
    29581 tgcagttttt gaatctcctc ctcacttaaa aagggaataa gcttcatcga ttcaactcct
    29641 ctcttatttt ttcaagccaa gcttgcttga gtttttctcc gaccagacgc ttgccatcag
    29701 ctaggtccaa cttttctagg aaacgggctt ggcccagatg gtaattggct tcaaagcctg
    29761 taatagcctg atgttgctgg acgtatgggg caatgcttct gccattttca gtataaggat
    29821 tgatggcgaa ccggcctgct aaaatcttct cagcagcttt cttgtaaaga taggcattgt
    29881 agtccagtag gagctgaaat tcctcatctg tcagttgatt cgccttgttt ttgttataaa
    29941 attcgcctaa ataactgctt tctttttcca agaagagccc ttggtatttc atagatttgc
    30001 tggcttctac cactgctcct gccagacttt ttaccgccat cagagattgg acaggttcag
    30061 ccatttccaa gtacatggcg ccgaaaaagt tctgctcccc ttctcttttt agggcagcaa
    30121 gataggttgg taactgagaa ttgagcccat taaagaaatg aggaaactgg aactgagtca
    30181 gactggattt gtagtctact actcctatcg ctccattagc tttcaaacgg tcaatccggt
    30241 ccaccttgcc tcgtacaaag acactgcgtc cattgtctaa ttgaataaag gcttggtctt
    30301 ttccaccaaa atttgcttct tctttgattg tttcgatggc tggattgtgt cggagaatat
    30361 gtccagttgt ccgtgcaaca tcaagcaaaa cttccttggt aaactgggct tccaaacttt
    30421 cttgataaat agcttcaaat tcgcgttctt gactggtttc ttgaatagct tgttctagac
    30481 gttggtcaaa ggaatcttca ttaggcaact gcaaggcgcg ttcaaagata cgatgcaaga
    30541 aattcccgtg actacgggca tcaggacgca aacgtaattc ctcctgcaag cctaaaacgt
    30601 agcgtaggaa ataactgtat tcattgcgat aaaactctgt caaacccgac gtagacaggt
    30661 aaaactcctg tttggcagga tagagagctt gcaaggtgtc cttggctaag gtcttgctgc
    30721 ttggactgat tgggatggct ggattttcca gaccttgctg atctagtttt ttacctatga
    30781 cacgcgacag aaccttgaca aaagtcaaat cttgctcagt atcgctcatc tcaccctgct
    30841 ggtgataggc aaccagacta gacaaaagac tgtgatagga ccccatatcc tccttagaca
    30901 gtcctttgtg attcatcctc ttctctctcc gcctaaatcc aaaatggatc aactcttgaa
    30961 gataggcaga ttccttactt tcactttcgt taaaaaggct tggagccgac aagaacaact
    31021 gcttacgagc agaattgacc aaggaaagca tagtgtagcg atttttcttg agattttcac
    31081 tgctggcaat cagtaattga acgccttctt cggtcgtttg gtttaggttt tgcctttctt
    31141 catctgtcag aagactggtg ttttgagaaa tttttggtaa attgtcctga gttagtccaa
    31201 tagcatagac aaagtcagca gtcaatggtg caatcaaatc gtaactctgc accagaacag
    31261 tgtccactgt tgctggaatg gtacggtatt gggacaaact cattccagaa tggagcaagg
    31321 ctaggaagtc ttccagacta acctgtgaac cagcaaaaac agtcgcaaat tgttctaaaa
    31381 catggcagaa agccttccaa acttcggctt gtctttcctg ttctacagct tccaaagtgg
    31441 ttgtcaaatc ttgtaactgc ttggtcacag ctccttcttt tagaaagaca ctccattttt
    31501 gtaggaggtt ttcagccttt tgttttcggc tggcaaagag ggtttcaaga ggtgctaaaa
    31561 ttctcaggcg gaggacattc aaacgctcaa gattaaattt tccatggtgg gatttggtga
    31621 aagtttgctg aaaggctggc aagccattga taccaagata gcggatatat tgctcaaaag
    31681 catcaatatc agactgactg aggtcagtat acaaatcagt tctaagaaga ttaatcaaat
    31741 cctcctgacg aaaacggtaa cgttttaaag ctaaaataga ctcgacaaac tgagtcaagg
    31801 gatgatgagc catggcttcg cttctaccaa gataaaaagg aatctgatac tggtcaaaaa
    31861 tggttttgag agataactgg taagaagcta catcccccaa gagaatacga aaatgcttgt
    31921 agctcaggtc tgagttctca tgtaatttct gacgaatgct acgggctact agctccaact
    31981 cctccttttg cgtcaaacaa gaccagattt gtaaattttc acggtctttc tcatcgacat
    32041 ccaaagcgag ttctgaaaag tcataagaag actccaacaa acgagaggcc ttgtcaaaac
    32101 tatccatctt ctcatgagtt tgagaacagt cctgagcagg cgtttggtat ttagaagcca
    32161 gatgatggag aaattttacg ctggcttggt agagattgcc ctcgctaaaa ggactggtat
    32221 aggctttctt actagcataa gccccgataa caatctcaac acctttgccg tgaagtaagt
    32281 ccacaacccg ctcttcctca gcagaaaaac gagtaaagcc gtcaatgacc aaggtgattt
    32341 gattaaaatc actacttacc ttgtcattct caatagcctc aatcaaatgg gacaactgac
    32401 ttccctgggc taactgacct tgattaagat aggctgttac tttctcaaaa atcaagagta
    32461 aatccgccct cttatcctca tctgttaaat tctccaagtc caaaaaactc atctgagatt
    32521 tggtcatctc atggtaaagc tcaattaact gctggatcaa ttgaggatcc tgcttaatag
    32581 cgccataaac acgcaagtcc ttgggatcga gttcggcaag gcatttgtaa aaggccaacc
    32641 caagaccgat atcatcaaga gtagttttaa ctggtaaatc attcaagacc agataacgag
    32701 ccatttgagc aaagcgcgtg acggtaatcg aaaaagaagc ctgctgggac aagcattcca
    32761 gcacggcgcg ttccttttca aaagaaagag agttgggggc aatgtagaag acccgcttgc
    32821 cagctgcaac tagctcttct gcctctcttg ttagaatttc tgtcaaagaa gttcgaatat
    32881 cagtataaag taatttcatc tcagcctcgt tggaattttt catcacccta tattatacca
    32941 tgattagcct cgtaaatctg ttaaaatatt taggccatcc tttcttttct tcatcatctg
    33001 ctaaatctta aatacttagc tttacttgta ttagatagaa taagtctggc tactgaaaat
    33061 cacataataa aaaagcctcg gtaacaaggc tttgagtttt atgattgttt cttaggtacg
    33121 gaatacactt caatgtgttg tcccagtatc ttaatgtcga ctggtagatt gtctgattta
    33181 tcgccatcaa catcggactc taattcgata tcagaagaag ttttaatatt acgtgccttt
    33241 atatattcaa tattcttgat agaatgattg aactatagta aattgaaact ataatagtac
    33301 accgtggatg ctaaaatatt tctagaaatt aatttgattt ccctaatcaa gctgttcgta
    33361 tcttatttca atcta
//
LOCUS       DAVBGY010000017        32919 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVBGY010000017 DAVBGY010000000
VERSION     DAVBGY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.017"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..338
                     /locus_tag="VQ620_000837"
     CDS             <1..338
                     /locus_tag="VQ620_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2763496.1"
                     /translation="CLSETADKNWHKSDRIFVTNTGKTVHSSILSKSLQRANERLKKP
                     IPKHLSPHIFIHITISILSENKIPLKTITDRVGHPDSEVTNSIYTHVTKNMKDEAINV
                     LDKVMKKIF"
     gene            complement(830..1240)
                     /locus_tag="VQ620_000838"
     CDS             complement(830..1240)
                     /locus_tag="VQ620_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763497.1"
                     /translation="MPKKKIERISVIHREKILWLKWYFMRDKEKPKYSVLECKMFDAA
                     KNKDMLAYKKYATIKQITDIRVQTSEDDILTAIKEVYVYNHMNVIGACQRILFVSQSP
                     AYNKLNKWFETYSDLYFSIIPLPNMGAYHELVDS"
     gene            complement(1258..1635)
                     /locus_tag="VQ620_000839"
     CDS             complement(1258..1635)
                     /locus_tag="VQ620_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763498.1"
                     /translation="MRKYLKEIKELQELKELLSSRNTPEVIIVEGNDDLGEFFQVDGE
                     LFSDIELLENLKKWREWEVQVIVDDWCNRGLNEYETGILYFPKHEDKMDYIRFNKGLE
                     PLYHALDEPYTTISKSEWLKLLD"
     gene            complement(1577..1992)
                     /locus_tag="VQ620_000840"
                     /pseudo
     CDS             complement(1577..1992)
                     /locus_tag="VQ620_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            complement(2036..2641)
                     /locus_tag="VQ620_000841"
     CDS             complement(2036..2641)
                     /locus_tag="VQ620_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763499.1"
                     /translation="MTFTTIKDDIKAFGKKKHGYMTGYIAKQEELHKQLQNGMIGEKY
                     AKDQLEAYKLEGDTYSRDTYNKIHAEIEKQHDLELEALKEKELSVTADDVAELTLLAS
                     MKMTKDELLGYFEKYKNKPLAIKKLWSIAEQYPEIAINLELFNAEQALESLILFFKRQ
                     LSYCHYSLLINGDKIQAVTTEMVVNSDAPELDRRLDEYLNK"
     gene            complement(2731..2904)
                     /locus_tag="VQ620_000842"
     CDS             complement(2731..2904)
                     /locus_tag="VQ620_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763500.1"
                     /translation="MTENKDNKLLEMMEKGFVLYSKNGIIKYIEIPDHGSIKLKAQDG
                     QLVYKEVTTGEQC"
     gene            complement(3503..4399)
                     /locus_tag="VQ620_000843"
     CDS             complement(3503..4399)
                     /locus_tag="VQ620_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET2763501.1"
                     /translation="MKSLQSSLGNIETFKLDELCPKHEIQLTQIKGERHVVVGWNENG
                     EVIKEVRCIPPYCEQCQEEQKKQDEEDAIADNLNAKLYLQTYNVLMSNKSYVADEFKT
                     KTFDDFNAVTSEEKRFLEFAKGQVQKYLDGMRGNTLITGGTGIGKTLLSVAIAKGINE
                     GYKTKGEPKSVLFVSLTEMIKQIKEGWNYGKGATLTEFEATELMKSVDYLIIDDLGAK
                     NAVIKPKSDWEQDLLFDVLNNRENTIFNTNLDSDELKIVYNERNYSRILKGLEGNTFK
                     AFTIKDKRYSINQLKNKVKQLK"
     gene            complement(4411..5220)
                     /locus_tag="VQ620_000844"
     CDS             complement(4411..5220)
                     /locus_tag="VQ620_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HET2763502.1"
                     /translation="MAKTKVYFWLKIDKKFFDNIFIKRLKTVPGGYTMTVIYIRLMLE
                     SLESDCILYYEGYFNNLKEELALKLDVSEDDIDMTMAYFTKCGLIQIDEDKNAELPQA
                     KAMVMSETNWASYKREQRKKGKLEEVQPSLTFSNSCPTEIEKELEIEVDKEQSPTPST
                     INQDFVNLYKSFEAETGKALSPLQIQELQYMLEDFSPELIHEALKEAVSQGKANFAYI
                     KAILNRWKQDNLLTVELVRNSRAAREAKKQQTNQLEPTSYEDWIPTQEKPF"
     gene            complement(5295..5510)
                     /locus_tag="VQ620_000845"
     CDS             complement(5295..5510)
                     /locus_tag="VQ620_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763503.1"
                     /translation="MNELDISNTQAVFVTVILIGLLLYLNHRDRKKSAQLERENQQTI
                     ETPSEDLNPDYGRYIQLAGVDVWGRIE"
     gene            complement(5512..5712)
                     /locus_tag="VQ620_000846"
     CDS             complement(5512..5712)
                     /locus_tag="VQ620_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763504.1"
                     /translation="MTLDLDNMTQAEFDEAMAEIQLQSPNIFQLISDFVNKKVTSVEI
                     DDLLNMKRAEQVAYIKNYKARA"
     gene            complement(5709..5900)
                     /locus_tag="VQ620_000847"
                     /pseudo
     CDS             complement(5709..5900)
                     /locus_tag="VQ620_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841301.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5889..6179)
                     /locus_tag="VQ620_000848"
                     /pseudo
     CDS             complement(5889..6179)
                     /locus_tag="VQ620_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            complement(6349..6501)
                     /locus_tag="VQ620_000849"
     CDS             complement(6349..6501)
                     /locus_tag="VQ620_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763505.1"
                     /translation="MKIVTFKPTKQIDGGFYLPGIDILFVSDKADAKSTEDVILFISR
                     NGLNKS"
     gene            complement(6512..6778)
                     /locus_tag="VQ620_000850"
     CDS             complement(6512..6778)
                     /locus_tag="VQ620_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763506.1"
                     /translation="MEILSKEIQLQGLQLLKQTLETLVELEKQRSSKLDLISRKELMD
                     LLGISATTLDNWEDLALKRYQTPMDGAKKVFYRLSDVYLFLAIK"
     gene            complement(6989..7171)
                     /locus_tag="VQ620_000851"
     CDS             complement(6989..7171)
                     /locus_tag="VQ620_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2763507.1"
                     /translation="MLITLSLAEKVRIKRARLQLTKKAVSEQLGIKSQTLTKVENGNY
                     DASKRIYESVMTWLVE"
     gene            7233..7736
                     /locus_tag="VQ620_000852"
     CDS             7233..7736
                     /locus_tag="VQ620_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2763508.1"
                     /translation="MYKMNRIKQLRKENNLTQRELANETKIPYRTIQRWENGETDIKS
                     DAAQVLADYFGVSIPHLLGYSDLIADTDSDIKAMAYSHLLTFVDEKQIKEFEKEFIRA
                     NDNSDTFGDLGSGEKALEKYYQALCYLVPPFRDILARYSFLSLDEKKSVYNIIVGLSN
                     NLSKENK"
     gene            7810..8973
                     /locus_tag="VQ620_000853"
     CDS             7810..8973
                     /locus_tag="VQ620_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET2763509.1"
                     /translation="MNIIEKIKKNGQRVYYASVYLGVDQLTGKKARTTVTATTKKGVK
                     VKVRDAINTFAANGYTVKDKPTITTYNELVKVWWDSYKNTVKPNTRQSMDGLVRVHLL
                     PVFGDYKLSKLTTPILQQQVNKWADKANKGEKGAFANYSLLHNMNKRILKYGVAIQVI
                     QYNPANDVIVPRKQQKEKAAVKYLDNKELKQFLDYLDTLNQSNYENLFDVVLYKTLLA
                     TGCRISEALALEWSDIDLESGVISINKTLNRYQEINSPKSSAGYRDIPIDKATLLLLK
                     QYKNRQQIQSWKLGRSETVVFSVFTEKYAYACNLRKRLNKHFDAAGVTNVSFHGFRHT
                     HTTMMLYAQASPKDVQYRLGHSNLMITENTYWHTNQENAKKAVSNYETAISNL"
     gene            complement(9127..10431)
                     /gene="eno"
                     /locus_tag="VQ620_000854"
     CDS             complement(9127..10431)
                     /gene="eno"
                     /locus_tag="VQ620_000854"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET2763510.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            10595..11041
                     /locus_tag="VQ620_000855"
     CDS             10595..11041
                     /locus_tag="VQ620_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HET2763511.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(11038..12153)
                     /locus_tag="VQ620_000856"
     CDS             complement(11038..12153)
                     /locus_tag="VQ620_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET2763512.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIDVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGRHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(12162..12491)
                     /locus_tag="VQ620_000857"
     CDS             complement(12162..12491)
                     /locus_tag="VQ620_000857"
                     /inference="COORDINATES: protein motif:HMM:NF019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET2763513.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGGNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(12445..12807)
                     /locus_tag="VQ620_000858"
     CDS             complement(12445..12807)
                     /locus_tag="VQ620_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HET2763514.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHC"
     gene            complement(12866..14299)
                     /gene="glgA"
                     /locus_tag="VQ620_000859"
     CDS             complement(12866..14299)
                     /gene="glgA"
                     /locus_tag="VQ620_000859"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET2763515.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(14296..15435)
                     /gene="glgD"
                     /locus_tag="VQ620_000860"
     CDS             complement(14296..15435)
                     /gene="glgD"
                     /locus_tag="VQ620_000860"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HET2763516.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVRTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(15425..16567)
                     /locus_tag="VQ620_000861"
     CDS             complement(15425..16567)
                     /locus_tag="VQ620_000861"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET2763517.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(16557..18485)
                     /gene="glgB"
                     /locus_tag="VQ620_000862"
     CDS             complement(16557..18485)
                     /gene="glgB"
                     /locus_tag="VQ620_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET2763518.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEYGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDIYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEVIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
     gene            18963..19760
                     /locus_tag="VQ620_000863"
     CDS             18963..19760
                     /locus_tag="VQ620_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HET2763519.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            19908..20030
                     /locus_tag="VQ620_000864"
     CDS             19908..20030
                     /locus_tag="VQ620_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET2763520.1"
                     /translation="MTCDFKFETLQLHAGQVVAPATKSRAVPIYQTTSFIFDDT"
     gene            20052..21092
                     /locus_tag="VQ620_000865"
     CDS             20052..21092
                     /locus_tag="VQ620_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HET2763521.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLG
                     NSLINIPDLEKLAEIAHKHQIPLVSDNTFAIPYLINVFSHGVDIAIHSATKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTCDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKDVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            21263..22537
                     /locus_tag="VQ620_000866"
     CDS             21263..22537
                     /locus_tag="VQ620_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HET2763522.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKDFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            22547..23425
                     /gene="truB"
                     /locus_tag="VQ620_000867"
     CDS             22547..23425
                     /gene="truB"
                     /locus_tag="VQ620_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET2763523.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEIILGYSTTTEDASGEVVAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            23693..23968
                     /locus_tag="VQ620_000868"
     CDS             23693..23968
                     /locus_tag="VQ620_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET2763524.1"
                     /translation="MEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQSVMANLSQGL
                     SLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            24076..24714
                     /gene="udk"
                     /locus_tag="VQ620_000869"
     CDS             24076..24714
                     /gene="udk"
                     /locus_tag="VQ620_000869"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET2763525.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            24842..26182
                     /locus_tag="VQ620_000870"
     CDS             24842..26182
                     /locus_tag="VQ620_000870"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET2763526.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            26160..26372
                     /locus_tag="VQ620_000871"
     CDS             26160..26372
                     /locus_tag="VQ620_000871"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET2763527.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            26369..27244
                     /locus_tag="VQ620_000872"
     CDS             26369..27244
                     /locus_tag="VQ620_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET2763528.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLESLQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            27237..28052
                     /locus_tag="VQ620_000873"
     CDS             27237..28052
                     /locus_tag="VQ620_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET2763529.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHSVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            28045..28476
                     /locus_tag="VQ620_000874"
     CDS             28045..28476
                     /locus_tag="VQ620_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2763530.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            28483..30150
                     /gene="recN"
                     /locus_tag="VQ620_000875"
     CDS             28483..30150
                     /gene="recN"
                     /locus_tag="VQ620_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET2763531.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRTIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLDLNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            30152..30880
                     /locus_tag="VQ620_000876"
     CDS             30152..30880
                     /locus_tag="VQ620_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HET2763532.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYSRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            30959..32782
                     /gene="lepA"
                     /locus_tag="VQ620_000877"
     CDS             30959..32782
                     /gene="lepA"
                     /locus_tag="VQ620_000877"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET2763533.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKMDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         9777 a         6511 c         6526 g        10105 t
ORIGIN      
        1 attgtctaag cgaaacagcc gataagaatt ggcacaaaag cgaccgtatt tttgttacca
       61 atacaggaaa aacagttcat agttctatct tgagcaagtc tctccagcga gcaaacgaac
      121 gccttaaaaa accaattccc aaacatctgt cccctcacat cttcatacac atcactatta
      181 gcatcttatc agaaaataaa attcctttaa aaacaatcac ggacagggtt ggtcatcccg
      241 actctgaagt cactaattcc atctacaccc acgtcacaaa gaacatgaaa gatgaagcaa
      301 tcaatgtact ggataaagtt atgaaaaaga ttttttaaaa agttttgtcc cttttttgcc
      361 ctctaaatac aaaaatagcc cttcggataa aatccgaggg gctagaaacg ttgttaaatc
      421 aacggccgaa cttttgaatt tcatggttcg ggataaaata gttcactgaa ctattttatt
      481 ttttaaggtt atcataatat caaatagttc aattaaatac gctaaattac taatatactt
      541 tttacctttt tcattctaaa atgtaaagta caaacaatta caatatacta gagggggagt
      601 aaaaaaggta ataaaattga cgagttcagc aggcaaggga aatagcactt ttcatggtgt
      661 tattttttta attcagcaat ggagtagccg cttaattgat agaaacaaaa agcacctagt
      721 ttcctagatg ctagcacaat agcccggatt cgaaccggaa tctcctcaat taaggtgcaa
      781 tccctatata ctactcattg tgctttggtt cattatatca tacaatcaac tagctatcta
      841 ccaactcatg atacgctccc atattcggta atggaataat actaaaatac aaatctgagt
      901 aagtttcaaa ccatttattc agcttgttat aggctggcga ctgactaaca aataatatcc
      961 gttgacaagc tccgataaca ttcatgtgat tatacacata gacctcttta atggccgtta
     1021 aaatatcgtc ttcacttgtc tgtaccctaa tatctgttat ctgcttaatc gtagcgtatt
     1081 ttttataagc tagcatatct ttatttttag cagcatcaaa cattttacac tcaagaacac
     1141 tatacttagg tttttcttta tctctcatga aataccactt gagccataaa attttttctc
     1201 tgtgaattac tgaaatacgt tcgatttttt tctttggcat gattacctcc taaattatta
     1261 atctaatagc tttaaccact cactttttga gattgttgta taaggttcat ctagcgcgtg
     1321 atataaaggt tctaaacctt tgttaaatcg gatatagtcc attttatcct catgctttgg
     1381 aaaatataag attcctgttt catattcatt tagaccacgg ttacaccaat catcaacgat
     1441 aacctgcact tcccattcac gccacttttt aaggttttct aaaagttcaa tatcactaaa
     1501 taactcacca tcaacttgga aaaattctcc cagatcatcg ttaccctcga cgatgataac
     1561 ttcaggcgta tttctactac ttaataattc cttgagttct tgtaactctt tgatctcctt
     1621 tagatacttc ctcaatttct aacctcaatt cttcaatttg tcttactact ccaaaaattt
     1681 catgagtttt atatgattgt tcaagtatag tttttgctgc ttgagttctt ataaaagggt
     1741 taattttggc atccatcata atatcattga gtacagaaac agcattggat gaggctagat
     1801 atagcatctg tgtagtttta tccatcatct catcttgttt tagtcaacag ctgcaacttt
     1861 tagatactta tgccctgttg cccttgacac ccctgctttt tggcacgctt tatctatcgt
     1921 tggctcagta agcatggcat ctatgaattt tatttgttta gatgtaaggc catcattttc
     1981 atttcctgcc atctattacc tcctcgttat caaaataaag gggttgctag cccctctatt
     2041 tgtttaaata ctcatccaaa cgacggtcaa gctcaggggc atctgaattt actaccattt
     2101 cagttgttac agcttggatt ttatcgccgt tgataagcaa actgtaatga caatatgaaa
     2161 gttgccgctt aaagaataag attagacttt ctagcgcctg ctctgcattg aataattcca
     2221 agttgatagc aatttctgga tattgttcag caattgacca aagtttttta atagcaagag
     2281 gtttgttctt gtatttttca aaataaccta gcaattcatc ctttgtcatc ttcatactag
     2341 ctaacaaggt cagctctgcc acgtcatcag ctgttacgct gagttctttt tcttttaatg
     2401 cctctagctc tagatcatgc tgtttttcaa tttctgcatg aattttatta taggtgtccc
     2461 tagaataagt gtccccctct aacttataag cctctagctg gtctttagcg tatttttcgc
     2521 ctatcatacc attttgtaac tgtttatgta attcctcttg tttagcaatg tagccagtca
     2581 tgtacccatg ctttttcttg ccaaaagcct taatatcatc cttgattgtt gtaaatgtca
     2641 tgttatcctc cttacgaaaa aataaaagag gtatgacaaa agagcgttaa actcaattat
     2701 catacctctg gtttttccag tcagtattta ttaacattgt tctccagtgg tcacttcctt
     2761 ataaactaac tgcccatctt gagcttttag cttaatgcta ccatgatctg gaatttcaat
     2821 gtactttatt ataccatttt ttgagtataa aacaaagcct ttctccatca tttctaataa
     2881 tttattatcc ttgttttcag tcatgaattt ctcctttatt tcaagtgtcc caacgggtac
     2941 ggttgtaagc tgagacattt tagggctatc tttgagcgac aaaaatgtcg aaaagtcttc
     3001 agttcctgta taaccgttat tagttttgtt tgctactcat ttttgagtca caaatctgtt
     3061 aagcaaaaat gagtaatagc tcgtaagata agtaaatcta atacttgtat tcctaaagcg
     3121 ttgatataac tgatattttc aactatccaa cgctctttac tacccttttt agtgtgcttt
     3181 agctcatttt tgagcttttt tgagcttttt tgagttccat tttgggaatg caaagcaggc
     3241 gcgcaccgtg catgcttttt tcttgctcat agcctcaccc tcagaagttt gccgaccgag
     3301 agcgtggggc tttttatttt tgtataatta aaaacagctg cacaattttt gtacagctac
     3361 gaccgaggca ggggcgacct gccgtacttt acactagata atatcatcta ggtagtccaa
     3421 ctgtatttta tcatattaaa ggctacatta caatttctca ccctgtgaag agtgtctatt
     3481 tttgtttagg tttgtaagtc gattacttca actgctttac cttatttttt aactgattaa
     3541 ttgagtatcg cttatctttg attgtgaaag ccttaaaagt gttaccctca agacctttta
     3601 gaatacggct ataattccgc tcgttgtata ctatcttgag ttcatcgcta tcaagatttg
     3661 tattaaatat agtattttct cgattattca gtacatcaaa aagtaaatcc tgctcccaat
     3721 cgctcttagg tttaataaca gcattttttg cacctaaatc atcaataatt aagtaatcaa
     3781 cagacttcat gagttcagta gcttcaaact ctgtaagtgt tgcaccttta ccataattcc
     3841 acccctcttt aatttgtttg atcatttcgg ttaggcttac aaaaagcaca ctcttaggtt
     3901 ctccttttgt cttatacccc tcatttatac ctttggcaat agcaactgat aaaagtgttt
     3961 ttccaatccc tgtacctcct gtgataagcg tatttcccct catgccatca agatattttt
     4021 gtacctgacc ttttgcaaat tctaaaaatc gcttttcttc tgatgttaca gcattaaaat
     4081 catcaaaagt tttagtttta aactcatctg ctacatagct cttattgctc atcaacacat
     4141 tataagtttg cagatatagt ttagcattca aattatcagc aatcgcatct tcttcatctt
     4201 gctttttctg ttcttcttgg cattgttcac aatagggtgg gatacagcga acttctttta
     4261 ttacctctcc gttctcattc caccccacta ctacatgtct ttctcctttg atttgtgtta
     4321 gctgtatttc atgcttagga cacaattcgt ctagtttaaa tgtctcaata tttcctaaac
     4381 tagattgtaa tgatttcatt ttctgacctc ctaaaatggt ttttcttgtg ttggtatcca
     4441 atcttcatag ctggtaggct ctagttgatt ggtttgctgt tttttagcct cacgcgctgc
     4501 cctgctattt ctaacaagtt ccaccgtcaa taaattgtcc tgtttccaac ggttaaggat
     4561 tgccttgatg tatgcaaagt ttgctttacc ctgactgaca gcctctttta acgcctcatg
     4621 gataagctct gggctaaaat cttctagcat atactgcaat tcttgaatct gtaacggtga
     4681 caatgcttta cctgtctcag cttcaaaaga tttatagaga tttacaaaat cttgattgat
     4741 ggtagaggga gtgggtgatt gttctttatc tacttctatc tctaattctt tctctatctc
     4801 tgttggacat gagttggaaa atgtcaaaga tggttggact tcttccaatt ttcctttttt
     4861 tcgttgttca cgtttataac tcgcccagtt tgtttcactc ataaccatag cttttgcctg
     4921 tggtagttct gcgtttttat cttcgtcaat ctgtattaaa ccgcattttg taaagtatgc
     4981 catggtcata tctatatcat cttctgatac atctaatttt agggctaact cttccttgag
     5041 attgttaaag tagccctcat agtataaaat acagtcactt tccaagctct ctaacatgag
     5101 cctaatataa ataactgtca tagtgtaccc accaggtacc gtctttagtc tcttgataaa
     5161 aatattatca aaaaatttct tatcaatttt tagccaaaaa tagacttttg tttttgccat
     5221 cgtcctctcc cttgctcatt tataattctc taccttgatt agtcttaata ctcgcttgtc
     5281 attcattgtt agactcattc aattcttccc catacgtcta cccctgcaag ctggatatat
     5341 cgcccataat cagggtttaa atcctcgcta ggtgtttcta tcgtctgttg gttttctcgc
     5401 tccagttggg cgcttttttt gcggtctcgg tggtttaaat aaagcagtaa gccaatcaaa
     5461 atcacggtaa caaaaaccgc ctgtgtattg cttatatcta gttcattcat gttatgccct
     5521 cgctttgtaa ttcttgatgt atgccacttg ttcagctcgc ttcatattca acaagtcgtc
     5581 tatttccaca ctggttactt ttttatttac aaaatcagaa atcagttgaa aaatgtttgg
     5641 actttgtagc tggatttcag ccattgcctc atcaaattct gcttgtgtca tgttgtctaa
     5701 atctagtgtc attgcattgc ctcctcaaac ttctctataa gacaactttt atttgctttc
     5761 tgagttccat ttttagagtt aaaaagaata tattttaagg ttacagtagc ctctaaatac
     5821 tccttttcag catgctctat atacgcctgt tgctctgctt cgttctcaaa aaagtgctta
     5881 gcttagcgtt taaagaatgc tttgactagg tttagtttga attgccgtac tgcttcggta
     5941 ttctttagat aagtgatcag caaagtagcc tgttgctcgt tcagaatata ggatttttta
     6001 ggttgtcctc tagtgtctaa tttatggatt ttaaatccaa gtattcccaa ctcttcaaag
     6061 tcaaccttat tttctcttat taagcgtgtg atagtgtggt gttgtacgtc agcacattca
     6121 gcgatgatct cgcttgtggt atacggctct ttcttgccgt ccatgtagac tagttccatt
     6181 atggttctac ctcctgtata aatttagcta gcttactttt taattgtctc ctctagccct
     6241 ttttagcctc tacaacggtt ttggctaacg tgttagaggc ttttataacg gcctgtatag
     6301 ctgtctctat ttcctccgtg gaatagtagc tttgttttct aatatcattc aagacttgtt
     6361 taacccattt cttgaaataa ataaaattac atcttctgta ctcttggcat ctgctttatc
     6421 tgagacaaat agaatgtcaa tgcctggtag ataaaaccca ccgtctattt gtttagttgg
     6481 tttgaaagta actattttca tttcataact cctattttat tgctaaaaat aaatacacat
     6541 ctgacagacg atagaatact ttcttagctc catccatcgg ggtctgatat cgtttaagag
     6601 caagatcctc ccagttatca agggttgtag cacttatacc tagcagatcc attaattctt
     6661 tacgagaaat taaatctaac ttactagatc gttgtttttc tagctcaact aaagtttcaa
     6721 gagtctgttt aagaagctgt aaaccctgta actgtatttc tttagacaga atttccattt
     6781 atatcctcca ttttcctttt tataaattaa gttgtctttt tcctctaatt ttgataaaat
     6841 aagaagagat tatttagcaa agggggttgt ccctttgcta aatacctaac aaaaccacgc
     6901 gccttgcctt tgcttgagtt ttgttatttt tccttagccg tgtacgttgg tttggtcgcc
     6961 tgtacatggc tttttacttg cgttaaattt attctactag ccaagtcatc acgctctcat
     7021 agatacgctt agaggcatca tagtttccat tctcaacttt tgttagtgtt tgagatttaa
     7081 tacccaactg ctccgatact gcttttttgg ttagctgtaa tcttgcacgc ttgatacgta
     7141 ctttttcagc aagtgatagt gtaatcaaca ttggtttcac ctcccttttt tctccatttt
     7201 tggcattttg tgttataatc tctttagaaa ggatgtataa gatgaataga attaagcaat
     7261 tacgaaaaga aaataatctt actcaaagag aacttgctaa tgagactaaa atcccttata
     7321 gaaccataca acgttgggag aatggggaaa ctgatataaa atccgatgct gctcaggtac
     7381 ttgctgacta ttttggagta tcaattcctc acttactagg ttatagcgat ttgatagcag
     7441 atacagatag tgatattaaa gcaatggcat actcacatct actaactttt gtagatgaaa
     7501 aacaaatcaa agaatttgaa aaagagttca ttcgtgcaaa cgataatagt gatacttttg
     7561 gtgatttagg aagtggtgaa aaggcgttag aaaaatacta tcaagcatta tgctatttag
     7621 ttcctccttt tagggatata ttggctaggt atagcttttt gtcactagat gaaaaaaaat
     7681 cagtctataa tattattgtt ggtttaagta acaacttatc aaaagaaaat aaataacttt
     7741 aaacatcatg tataaatacc taacaaaacc acgcgccttg cctgctgatg gaaagaaagg
     7801 tacaaataca tgaatatcat agagaaaatt aaaaagaatg gccaaagagt ttattatgct
     7861 agtgtttatc taggcgttga ccaactaacg ggcaaaaaag cccgtacaac tgttacagca
     7921 accactaaaa agggcgttaa agtaaaagtg cgtgatgcga tcaatacttt tgctgctaat
     7981 ggctatacag ttaaagataa gccgacaatt acaacataca atgagcttgt aaaagtttgg
     8041 tgggatagtt acaagaatac agttaagcca aatactcgcc aatccatgga tggattggtt
     8101 agagtgcatt tattgcctgt atttggcgat tacaagctat ctaaacttac tacgcctatt
     8161 cttcaacagc aagtaaacaa atgggctgac aaggcaaata aaggcgaaaa aggggcattt
     8221 gctaactact ccttgctcca taacatgaat aagcgtattt tgaaatatgg cgtagctatc
     8281 caggtaatac aatacaaccc agctaatgat gtcatcgttc cacgcaaaca gcagaaagaa
     8341 aaggctgctg tcaaatactt agacaacaaa gaattaaaac agtttcttga ttatttagac
     8401 actctgaatc aatcaaatta tgagaactta tttgatgttg tcctgtataa gactttattg
     8461 gccactggtt gccgtattag tgaggctcta gctcttgaat ggtctgatat tgacctagaa
     8521 agcggtgtta tcagcatcaa taagacacta aaccgctatc aggaaataaa ttcacctaaa
     8581 tcaagcgctg gttatcgtga tataccaata gacaaagcca cattactttt actgaaacaa
     8641 tacaaaaacc gtcaacaaat tcagtcttgg aaattaggcc gatctgaaac agttgtattc
     8701 tctgtattta cggagaaata tgcttatgct tgtaacttac gcaaacgcct aaataagcat
     8761 tttgatgctg ctggagtaac taacgtatca tttcatggtt tccgccatac acatactact
     8821 atgatgctct atgctcaggc tagcccgaaa gatgttcagt atagattagg ccactctaat
     8881 ttaatgatca ctgaaaatac ttactggcat actaaccaag agaatgcaaa aaaagccgtc
     8941 tcaaattatg aaacagctat cagcaattta taaaaaataa gggtgaccca tttccgggct
     9001 accctcttac tataccaaaa attagtaggg gtagtaaaaa gggtattaaa ttataaaaag
     9061 cactaaggga aagcgcccca aagtgcttat ttcaaaggct ttatagccta taatcacata
     9121 aggagattat tttttaaggt tgtagaatga tttcaatcca cgatattcag ctacttcacc
     9181 aagttggtct tcgatacgaa gcaattggtt gtatttagcg atgcggtctg tacgtgaaag
     9241 tgaaccagtc ttgatttgtc ctgcgttagt tgcaactgca atatcagcga ttgttgaatc
     9301 ttcagtttca cctgaacggt gtgatacaac agcagtgtaa ccagcttctt tagccatttc
     9361 gatagcttca aaagtttcag taagagtacc gatttggtta actttgataa ggattgagtt
     9421 agcagcacct tcttggatac cacgtgcaag gtagtcagtg tttgttacga agaagtcgtc
     9481 accaacaagt tgtactttct taccaagacg ttcagtaaga gctttccaac catcccagtc
     9541 gttttcatcc ataccatctt caatagtgat gattgggtat ttgttaacca attcttcaag
     9601 gtagtcgatt tgttctgcag atgtacgaac agcagcacct tcaccttcaa atttagtgta
     9661 gtcgtaaact ttacgttctt tatcgtagaa ttctgatgaa gcacagtcaa atccgataaa
     9721 tacgtcttta cctggtacat atccagcagc ttcaatcgca gcaaggatag tttcaacacc
     9781 atcttcagtt ccttcgaaac gaggagcgaa tccaccttcg tcacctacgg cagtttccaa
     9841 accacgtgat ttaaggattt tcttaagagc gtggaagatt tcagcaccgt aacgaagggc
     9901 ttctttaaat gttggcgcac caactggcaa gatcatgaac tcttggaaag cgattggagc
     9961 gtcagagtga gaaccaccgt tgatgatgtt catcattgga gttggaagaa ctttagtgtt
    10021 gaatccacca aggtagctgt aaagtgggat ttcaaggtag tcagcagcag cacgagctac
    10081 agcgatagac acaccgagga ttgcattcgc acccaattta cctttgttag gagtaccgtc
    10141 aagtgcgatc atagcacggt caatagcttg ttgatcacgt acatcgtagc caatgatagc
    10201 ttcagcaatg atgttgttta cgttgtcaac agctttttgt gtaccaagac caccgtaacg
    10261 agatttgtca ccgtcgcgaa gttcaactgc ttcgtgttca ccagtagaag ctcctgatgg
    10321 aaccatacca cgtccgaaag cacctgattc agtgtaaact tctacttcaa gtgttgggtt
    10381 accgcgtgag tctaggactt cgcgagcgta aacatcagta ataattgaca ttttttactc
    10441 tccttatgag ttaaattttt tacacctcta taatacctta aaacccctcc tttttcaaga
    10501 aaaaacgtta tctttgtgca acttttcctt aactttataa agtaatcgct ttcttttgtc
    10561 tgttttattc taacttttat gatatactgt tttcatgaca gatttatcaa aacaattact
    10621 tgaaaaagct catggtgggt taaaaataaa tccggatgag caaagacgct atcttggtac
    10681 ttttgaggaa agagttcttg gatatgtaga tattgacaca gcaaatagcc ctcagttaga
    10741 aaaaggcttt ttatttattt tagaaaacct tcaggaaaaa gcagagccac tatttgtgaa
    10801 gatttcacca actatcgaat ttgataagca agttttctac ttaaaagaag caaaagaaac
    10861 tgatagtcaa gccaccatag tatctgaaga gcatattact tctccttttg gcctggttat
    10921 tcatagcaat gcaccagttc aagtagaaga aaaagacctt cgacttgctt ttccaaaact
    10981 ttgggaagtt aaaaaggaag aaccagccaa aacatcctta tggaagaaat ggtttagcta
    11041 aatcttgcac atatttaata agtgcccaat attggcagcc gtgcgctcca gatagagact
    11101 ggcatttttc aaactatctt ctaaaggttc acttttctcc aaaatagaaa agacagcttg
    11161 gatattttca aatggtaggg gaggtaaatc ttcagcaaga ctaccgcaaa tagcaataac
    11221 aggaactcca acaggggttc tttttgcaac acctataggc gctttcccag caaagctttg
    11281 actatcaagt cttccttctc caacaacaac caagtcagca tctgaaactt tcttatcaaa
    11341 gttgattaag tccaagcagg tatcaattcc agacacgata cttgcctgag caaaagcaca
    11401 caaaccacca gcaaggcctc cacctgctcc tgctccttta atttctaatg ttgcaggtga
    11461 gaatttttca taaaaatctt ggatcgcctg atctacgact gcaaacatag tcggatgtcg
    11521 acctttttga ttgccaaaag tataagtcgc accttgatga ccacataagg gacttacgac
    11581 atctgctaaa atatgaattt gaacaccttc aggaatttta tagcaatttt ctgttgaaac
    11641 agaagctaag tttaataagg attgaccgga agcaggcaag acatttccat ccctatcata
    11701 aaattgataa cctaaaccag cagcaatccc cagtcctcca tcattactgg ccgtgccacc
    11761 aacatcgata taaatatctt taatcccttt agaaatgaga tggagaatca actctccaat
    11821 accacaagtt tggatttgaa gtggatttcg tttctctagc ggaatttttc caagaccaac
    11881 caagtcagct acttcaaata gtgccagttc ccctttttga aaatagcgca tggcttcttt
    11941 ttgtccaaaa gggtctgtca cttggatcca tttttctttt aggtcaagag aatgtcggat
    12001 agcatctaca gtaccttctc ccccatcacc aacagggcag aggagacatt ctacatctgc
    12061 tatcgattgt tggaagcctc tttttattgc ttcagctacc tgttgagctg tcaagctttc
    12121 cttaaacgaa tccggtgcaa ttacaatctt catattttcc ctcattctaa acagtcaatc
    12181 aaaggaagaa cttctaaaaa atccctcttg tcaacatgat gtggtatttc ttttttgagc
    12241 atttctttgg aacaaaaggc gattcctaac tccgccgact tcaacattaa tagattattg
    12301 cccccatcac cgattgccac cgttctttct ttagaaagtt ttagtttctt tctccatttt
    12361 tccagagttt cttttttgac ctggggactt ataatttgtc caactaattt tcctgttaaa
    12421 agaccttctt tgacttcaag ctggttagca gtgaaatagg caataccaag ggattttgct
    12481 aatctctcca actattggtg taaatccacc agacaccaga ccaactagga taccattctt
    12541 ttggagaata gagatgaact ctgggacatt tagcgataga tgaattgagt tgaagacgtt
    12601 atcaaagacc aaaataggaa gaccttccaa caaggacact ctttttctta aactgctttc
    12661 aaagaccaac tctcctcgca ttgctcgact tgtaatctgc gaaatttctg cctcatgacc
    12721 tgcctctctc cctaaaagat caatcacttc ttctaggatt aaggttccat ctacatccaa
    12781 aacacacaag ccttttactt gagacatcag ttctcctctc taaacagcct aaaaatcgta
    12841 tgaagtcatc atacgatttt atctattaat taactaaact atggtacaag tcaaggtatg
    12901 acttgcaggc tgtatcccat gagaagtcac tctccatagc ttgtttttgt aggtttctcc
    12961 aaatgtctgg atggtttcta tacaagtcca atgctgtttg gaaagtccaa tttaaccaat
    13021 aaggagatag attgtcaaag ctaaagccag taccgcttcc ttcgattgga ttgaaagcgc
    13081 gaactgtatc tcgcaagcct ccaacttcat ggaccaatgg caaggttcca taacgcatag
    13141 ccatcatttg agacaagcca cacggttcaa aacgacttgg catgaggaag aggtcacaag
    13201 cagcgtagat ttcctgagca agtttgacat caaaagtgat atttgttgat agcttgtctg
    13261 ggtaaatctg agcaaaccat gagaaagctc cttcaaaggc tggatcgcca gttcccaaaa
    13321 gaacaatctg aacatcttct tgcaagatat ggtgaagact ttcgaccacc acatcaaaac
    13381 ctttttgacg tgtcaaacga gaaacaattc ccaccagtgg aacgtctgct ctaacaggca
    13441 agccaactct ttcttgcaat tttgccttat ttttggcttt cccagacaaa tcttcctgat
    13501 tgaaatgata gtctaaaaga gcatccgtct gaggattata aagatcagca tcaatcccat
    13561 tcacgatacc agatacttta ccagactcca ttttaagaat ctgatccaaa ttacatccaa
    13621 actgactagt cataatttca tgagcatagc taggtgaaac ggttgaaaca cggttcgcat
    13681 agagaatacc tgccttcatc cagttcagac agttgttcca tcgaagggtg ccatcagcgt
    13741 aacgttcaaa gccaactcca aacaaatcac ccaacattcc ttctgaaaat tgtccttgga
    13801 attctaaatt atgaatggtt aaaactgttt caatgtcctc ataggcttga atccaacggt
    13861 atttttcctt caacaagaaa ggaatcatag ctgtatggta gtcatgaaca tggagaagat
    13921 caggaataaa gtcaatcctt tccatagcct caatggcagc cagttggaaa aaggcaaagc
    13981 gttctccgtc atcaaaatca ccgtaaacat gaccacggaa gaaataatat tgattgtcaa
    14041 taaagtagaa ggttacacca tttaatactg ttttcttaat tccacaatac tgtctgcgcc
    14101 aaccaacgct cacctcaaaa tgaagcacat cttcaatctg atttccaaat ttagcctcta
    14161 ccatatcata gtagggtaaa atcactgcaa cttcgtgccc agcttttacc agtgattttg
    14221 gaagagcgcc aatgacgtct cccaaaccac ctgtttttga aaagggtgca ccctctgctg
    14281 ctacaaataa aattttcatg aatgaatatc ctctgttact ttagcacctt tcttaaccac
    14341 aactggatgt tctgcagttc ctcgaatcac aacaccaggc tcaacttcaa cccctttgtc
    14401 caagatagca tattcgacct gagccccttc tccaataaca acacgaggga agagcaggct
    14461 atctttaacc aagctatcct tatggacatg gatattacgt gatagaacag aattagctac
    14521 ttgaccttca ataatactac cagaggcaaa ctgagaagtt cttaccttag atgtattagc
    14581 atagtaagtt ggctcttcgt ttttgacctt tgtataaatc ttttggtttg gtgagaaaag
    14641 agaatagaat ttttgtgatt caagcatatc gatattcgct tgataataag atttaacaga
    14701 gtgaatattg gctagatagc ccgtgtactc gtaggcgaaa gctccctctt ttacagccaa
    14761 atcccgtaaa acatagcgca atttctctgg atgttctttt ttagcttctt cttccaagtg
    14821 ttcaatcaac caaggtgtat caacgacaaa gatatctgta gacatattga acgtttcagc
    14881 tgttgacttg ctatcaaaga gtttatgaga aagaacatgg tctgtttcat ctacatccaa
    14941 gattgcattt acttctgaaa tatctttctt agctagtttt ttataaacta cagtgatagg
    15001 ctcttttgtt gtactatgta ggtggaaaac ttggttcaaa tcaatgttaa taagaacatc
    15061 gcagttgagg gcaaccgttt ggtttgagcc agaacgtttc aaataagtaa gaagctgttg
    15121 gtagtattct tttccaactg tactactttc tacacgggta ttgtaaattc ctagatagta
    15181 atggctaaga agggttgata agccccactc gcgtcctgaa cgaatatggt caaatactga
    15241 gctgatatta tcctgctgga aaataccaaa gacactacga acacctgcat tagcaaggct
    15301 tgaaagtggg aagtcaatca aacgatattt cccaccaaat ggcaaacttg ctactggacg
    15361 gtggtccgtc aatgtcgaca tattgtgaaa accaactgta tttcctaaaa tggcagaata
    15421 tttatcaatc ttcatctgtt gctaccccca ctacttcatt atatcctaca acttgtactt
    15481 catctgttcc atcaatttcg acaccgtcag aaataatcgc accttcacca ataatggcac
    15541 gtttaatctt agctccttga ccaatgatag ctccactcat gataactgaa tcaaggactt
    15601 ccgctccttc gcgaacttgc gcgcctgttg aaaggataga atgtttaaca gttccatcaa
    15661 cgaaacatcc gtctacaact aatgagtctt ccacatgagc atttgccccg aggaagtttg
    15721 gtggtgaaat caagtttctt gagtaaatct tccattgacg gttacgacta tccaaggcat
    15781 tttctggaga aatatactcc atgttcgctt cccaaagtga ctcaatagta ccaacatctt
    15841 tccaataacc actaaattcg taagcataaa cactttcacc tgactcaagg taatttggaa
    15901 tgacattttt accaaagtct gacatgccaa ccttgctctt ttcagcagcg actaacatat
    15961 tacgaaggcg ttgccaatca aaaatgtaga ttcccataga agcttttgta gatttaggtt
    16021 gagctggttt ttcttcaaat tcaacaatac gattgttagc atctgtgttc atgataccaa
    16081 aacggcttgc ttctttaaga gggacgtcta aaactgctac tgtcaagctg gcattattat
    16141 ccttatgaga ctggagcata tcatcatagt ccattttgta gatgtgatcc ccagacaaaa
    16201 tcaagacata ctcaggattg acactgtcga tatagtcgat attttggtaa atagcgtgac
    16261 tagtcccctc aaaccaacga tttccttcac ttgcagaata aggttgaaga atagagacac
    16321 ctgaattaat accgtctagt ccccagcttg aaccattccc aatatggttg ttgagagcaa
    16381 gtggttgata ctgtgtaacg accccaacat tatgaatccc tgagttggca cagtttgata
    16441 gggcaaagtc aatgatacgg tagcgcccac caaattgcac agctggtttt gcgatgcttt
    16501 gagtgagttt accgagacga gttccttgcc caccagcaag aatcaaagct aacatttcat
    16561 ttttcatttt ctactccttt ttggttttta tttgtgacgg ttttagtaga tttcaagcga
    16621 cgtttgattt tccatacact tgctcccata gccggtaggg taaaggttaa ggtctgctca
    16681 taatctttcc atagtccttc ttgcgtttga acagtttgat tatgttcttt ccaaacgcct
    16741 ccccactctt ccaactcagt attccatact tcttcgtaaa ttcctgcaac gggtagtccg
    16801 attgtaaaat ctttccgctc aacaggtacc atgttaaaga tacagactaa catttctccc
    16861 tttttaccct tacgaataaa ggaaagaaca ctctggtctc gattatccgc atcaatgact
    16921 tcaataccat catagctggt atcgatttcc cacagacagc gatgatcttt gtaaaactgg
    16981 tttagctgag aagtgaaata cttcatctta gcattcattg ggtcttctag gttagaccat
    17041 tccaactgtt cttcagattt ccattctagg aattgaccgt attcgctacc catgaagagc
    17101 aatttcttac cagggtgaca aatttggtac gtatagagat tgcgcaagcc tgcgaattga
    17161 ttgtaacgat ctccccacat cttatgcatc atactcttct tgccatgaac cacttcatcg
    17221 tgcgagaatg gcaagagata attctccttg aaaacataca taaagctgaa agtcaccagg
    17281 ttaaagtcat atttacgata aatcggatct tcttcgtaga aacggaggat atcattcatc
    17341 cagcccatgt tccatttgta gtcaaatcct agaccaccaa tctctttcat tcccgtaatc
    17401 ttggttgcag acgaactttc ttctgcaatc atcatcacat ctggatattc taacttaata
    17461 acttcattca agcgttgaag gaaataataa ccttcataat tgagatttcc gccatcttta
    17521 ttaggtgtcc atggagcatc atcatagtcc aaatagagca tgttgctaac agcatccaca
    17581 cgaataccat ccaaatgata gatatcaatc caatgcttaa tgcaagaaat taagaaggac
    17641 tggacttcat tttttccaag gtcaaaatta agggcacccc aaccatggtt atgagcctta
    17701 ttatggtctt ggtattcaaa agtcggtgtc ccatcataat aggctaaggc atcatcattg
    17761 atggtaaagt gacctggtac ccagtccaca ataaccccaa tattatgggt atgacactcc
    17821 tcgacaaaat cttgaaactc ctctggtcta ccataggcat gctctaaagc gaagtaaccc
    17881 ataagctggt acccccaact caaacctagt gggtgggaca tcaagggcat aaactcaata
    17941 tgagtatagt tcatctcaac taggtaagga atgagttcat ctttcaattg ggcaaaacta
    18001 taaggactgc catcaggatt tctcttccaa gagccagcat gaacctcgta aatattgaca
    18061 ggacgttcag caaagcccca acgttttctt cgtgccagcc aaagtccatc cctccatttc
    18121 ttatcaggaa gctctgttac gattgcccct gttcctggac gagcctcata cctgacagca
    18181 aaagggtcaa tcttcatcag ttgatgacca ttttgacgtg tgacatgata tttgtaaata
    18241 tgcccttctt gagccatatt ggtaaagact tcccagaccc caaagtcatt tcttaccatt
    18301 ggaatctgat tttcaatcca attggtaaaa tcaccaacca agtgaacagc ctgagcatta
    18361 ggtgcccaaa cacggaaggt atagccatac tctccattta gttcttccct atgtgctcct
    18421 agataatgtt ggagataaaa attttcaccc gtcataaagg tttttaatgc ttctctatta
    18481 tccatatact ccctttctcc tgtaagcgtt ttctatgttt ttattatact acctttttag
    18541 agaagattca agtaaattac tatacttctt taattatttt gaaaatctac aacaagttca
    18601 cttactcgtt caattgtaaa tcaatatttt ttcaaaaaat tgcgaaaaca cctttctttt
    18661 tctactatag tgaaatgaaa taaaacatgc gcaaatcgat taaggaattt aatctaattt
    18721 ctaacaatgt cttagaaatc aaagtgtact attttaactt caatgtacta tatatagtaa
    18781 aatgaaataa taacaggaca aatcgatcag gacagtcaaa tcgatttcta acaatgtttt
    18841 agaagtagag gtgtactatt ctagtttcaa tctactataa acttttatga aatacagaaa
    18901 acgcttccta aaacctaaag tttgtgatat aattatcaag aaaaaagatt taggagttta
    18961 ttatggtttc ttcagaattt atctcaaaga ttgaatttgc ttgcaataag aaagaaagtc
    19021 tttatagtca aagcaaattt aagtatgcga ttcgttcgat gttcgcaggt gcatttttaa
    19081 ccttcagtac tgctgcaggt gcagttgggg ctgacttgat taataaaatt gcaccaggta
    19141 gtggacgctt cctctttcca ttcgtttttg cttggggctt ggcctacatt gtttttttga
    19201 atgccgagtt ggtcacttca aacatgatgt tcttgactgc tggtagtttc ttaaaaaaaa
    19261 tctcttggag aaaaacagct gagattttac tatactgtac cttgttcaac cttatcggag
    19321 ccttgatagc agggtggggc tttgctcatt cggcagccta tgcgaatctg acacacgata
    19381 gtttcatctc aggtgttgtt gagatgaagt taggccgctc aaatgaattg gtcttgcttg
    19441 aggcgatttt ggcaaatatt ttcgtaaata ttgcgattct gtcatttatt ttggtcaaag
    19501 atggtggtgc caaactttgg cttgtgttgt cagctattta catgtttgta ttcttaacaa
    19561 acgagcacat tgcggcgaac tttgcttctt tcgcgattgt gaaattcagt gttgctgcgg
    19621 attcaattgc caacttcggt gttggaaata tgcttcgcca ctggggtgtg actttcatcg
    19681 gaaactttat cggaggaggc ctcttgatgg gtcttccata tgccttcctc aataaaaacg
    19741 aagatactta tgtagattaa gaaaatgagc acgattgagt cgtgcttttt tcattttcaa
    19801 aataaggtaa tagctatttc ttatatcaaa atatagaaaa ctgatatttg taaactataa
    19861 ctcaaggtgc tacaatatcc ttaataaaat gatatggagg tcaccttatg acttgtgatt
    19921 ttaaatttga aactctacaa ctacatgctg gtcaagttgt ggctccagct actaagtctc
    19981 gtgcagtgcc gatttatcaa acaacatctt ttatttttga tgacacgtag gaaggtgctg
    20041 atctgtttgc cttgaggaaa ccagggaaca tttatactcg tatcaccaat cctacaacag
    20101 ctgcccttga aggtggtgtt gaagcgctag caacagcatc aggtatgact gcagtgactt
    20161 atacgatttt ggcgcttgcc catgctggtg accatgtagt ggctgcttcg actatttacg
    20221 gtggaacctt caatcttttg aaagaacccc ttcctcgtta tggtatcaca acaacctttg
    20281 tcgatgttga taatttggag gaagtagaag cagctatcaa agacaatacc aagcttgtct
    20341 tgattgaaac cttgggtaac tccttgatta atattccaga cctggaaaaa ctggcagaga
    20401 ttgctcataa acatcaaatc ccacttgtgt cagacaatac ttttgcaata ccttatttga
    20461 ttaacgtctt ctctcatggc gttgacattg ccattcactc tgcgactaag tttatcggtg
    20521 ggcatggtac aactattgga ggaataattg tcgatagtgg tcgttttgac tggacggctt
    20581 cagggaaatt ccctcaattt gttgacgagg gtccaagctg ccacaatttg agttatactt
    20641 gtgatgtggg tgcagcagcc tttattatag ctgttcgagt tcaattgctt cgtgatacag
    20701 gtgcagcctt gtcaccattc aatgctttcc tcttgctaca aagacttgaa acctcttcac
    20761 ttcgtgtgga acgccatgta caaaatgctg agacaattgt tgattttctt gtcaaccatc
    20821 ctaaggtaga aaaggtaaat tatccaaaac ttgcagatag tccttatcat gccttggctg
    20881 agaaatactt gccaaaagat gtcggttcaa tctttacctt ccatgtcaaa ggtggcgagg
    20941 aagaagcacg caaggtcatt gataatttag aaatcttttc tgaccttgca aacgcggcag
    21001 atgctaaatc gcttgttgtc catccagcaa caaccactca cggtcaattg tcagaaaaag
    21061 acctagaagc agcaggtgtc acaccaaact aaattcgttt gtcaatcggt cttgaaaatg
    21121 tagaagattt gattgaagac ttgcgcttgg ccttggaaaa aatttaaagt aaaagaagat
    21181 aaacagtggg cttcgactca ctgtttttga ttttccctca ggcatgatat aatggttaaa
    21241 gaagtctaga aagaggaacg atatgaacga aatcaaatgt cccaactgtg gggaagtctt
    21301 tacagtaaat gagagtcagt atgccgaact cttgtcccaa gtgagaacgg cagagtttga
    21361 taaggaacta cacgatagga tgaagcagga actggccttg gctgagcaaa aggccatgaa
    21421 tgagcaacag actaaactgg ctcagaagga tcaagaaatt gcgcaattac agagtcagat
    21481 ccaaaacttt gatacagaaa aagaattggc caagaaagag gttgaacaga caagccatga
    21541 ggctctcttg gctaaggaca aggaagtaca gctcttagaa aatcagttgg ctaccttgcg
    21601 tttggagcat gaaaatcaac tgcaaaagac cctttctgac ctagaaaaag aacgggatca
    21661 ggttaaaaac caactacttt tgcaggaaaa ggaaaatgaa ttatctttgg cttctgttaa
    21721 gcaaaactac gaagcccagc tcaaggcagc tagtgaacaa gtcgagtttt ataaggattt
    21781 taaggctcaa caatctacaa aagcgattgg ggaaagccta gaacagtatg cagagagtga
    21841 gtttaacaag gttcgtagtt tcgcctttcc aaatgcttac tttgagaagg ataacaaggt
    21901 ctcttcgcgt gggtctaaag gggactttat cttccgtgag tgtgatgaaa atggagttga
    21961 aatcatttct atcatgtttg agatgaaaaa cgaagcagac ggaacagaga agaagcacaa
    22021 gaatgcagat ttttacaagg aattggacaa ggaccgtcgg gagaagaact gtgagtatgc
    22081 cgttttggtg accatgcttg aggccgataa tgactacttt aacacaggga ttgttgacgt
    22141 cagccacgag tatgaaaaaa tgtatgttgt tcgtcctcaa ttctttatcc agttgattgg
    22201 tctcttacgt aatgcggcgc taaattccct aaaatacaag caggagttgg ccttggttcg
    22261 cgagcaaaat attgacatta cgcattttga ggaagacttg gatgccttta agctagcttt
    22321 tgctaagaac tataattcag cttcgactaa ctttggaaaa gctattgatg aaatcgacaa
    22381 ggccatcaaa cgcatggaag aggttaagaa attcctgact acatctgaaa accaactccg
    22441 tctagctaac aacaaattgg aagatgtctc tgttaaaaaa ttgacccgga aaaatccaac
    22501 aatgaaagcg aagttcgaag cactgaaggg ggagtagaaa gcaaaaatga acggtattat
    22561 caacttaaaa aaagaagcag gaatgacctc gcatgatgcg gtttttaaac tgcgtaagat
    22621 tttgggaacc aagaaaattg gtcatggtgg aaccttggat ccggatgtgg tgggtgtttt
    22681 gccgattgcg gttggcaagg cgacacgcat ggtcgagttt atgcaggacg agggtaagat
    22741 ctatgagggg gaaatcattc tgggctattc cacgacgact gaggatgcta gtggggaagt
    22801 ggtcgcagaa acccctgttt tgtctccctt ggatgaaaag cttgttgatg aagcgattgc
    22861 tagcttgact gggcctatta ctcagattcc ccctatgtat tcggcagtta aggttaatgg
    22921 tcgcaagctc tatgagtatg cgcgtgctgg tcaggaagtg gagcgtccag aacgtcaggt
    22981 gaccatttat caatttgagc gaacaagtcc gatttcttat gatggccaac ttgcccgatt
    23041 cacttttcgt gtaaaatgca gtaaagggac ttacatccgt actttgtcag ttgatttggg
    23101 tgaaaagctt ggttatgcgg ctcatatgtc ccatttgact cgtactagtg ctgctggctt
    23161 acaattagaa gacgctctta ccttggagga aattgctgaa aaagtagagg ctgggcaatt
    23221 agattttctc catcctttag agattgggac aggtgacctt gtcaaagttt tcctaactcc
    23281 agaagaggct acagaagttc gctttggtcg ttttattgag ctagaccaaa cggacaaaga
    23341 actggctgcc tttgaagatg ataaattgtt agccattcta gaaaaacggg gcaatctcta
    23401 taagccaagg aaggttttta gctagatcgt ttaggaataa aaatcgggtg atagataaca
    23461 attgcttgat aaacccccat actaatagta gaatggtttt gggaattata atattccaat
    23521 tgttgcgagt tgtagatact caaataatct atatagaaat ttagaggtgt gaaatgaagc
    23581 aacttaaaat tctttcagat aaatatttag agtccatgac aggttctggt gggaacttag
    23641 gtctaggatt tggtgtgata attccatgat gtgaaatgag tttcgagaaa gggtggagca
    23701 acttcttcaa caaaaagaaa taaatgaaaa tagtgagttg agtcacctgt ttcgtcttgc
    23761 tatacaaaat ttagacagaa atgaaaaata ccaatcggtc atggccaatt tgagtcaagg
    23821 gttgtcactt tacctcatga cgcatcatta ccaggcacct aagtctgtca ttgattttgg
    23881 tttatggatt gccaaagctc ctagccagga aagagggaga ctggctttct tgcagatgct
    23941 tgctcaaacc ctacagggct ttcgttaaaa ctgtcgaaca tcagagggaa gactctggtg
    24001 ttttttgctg atgagactat aaattttgta aaaaatgtga tagaatagac gacggataaa
    24061 ataacggagg atagcatgca aaatagacca atcattattg gagtgacagg tggttctggt
    24121 ggtggtaaaa ccagtgtttc aagagccatt ttatcgcatt ttcctgatga aaagatttcc
    24181 atgattgagc atgattcata ctacaaggat cagtctcatt tgacctttga agagcgtgtc
    24241 aaaaccaact atgaccatcc ttttgccttt gatacagact tgatgatcga gcagattaag
    24301 gaattgttgg cagggcgtcc ggtggacatc ccgacctacg actatacaga gcatacacgg
    24361 agtagcaaga cctatcgtca ggagcctcaa gatgtcttta tcgttgaggg cattttggtc
    24421 ttggaggaca agcgtctgcg cgatttgatg gatatcaaga tttttgtgga tacggatgac
    24481 gatgtgcgta ttattcgtcg tatcaagcgt gatatggagg aacgtggccg tagccttgat
    24541 agcgttatta accagtactt aggtgtggtc aaaccaatgt accaccagtt tatcgagtca
    24601 actaagcgtt atgctgatat cgtcattcct gaaggggtta gcaataccgt ggctatcgac
    24661 ctgttgacga ccaagattgc aaagattttg gaagaagctc gaaacagcaa ataatcacat
    24721 gtggaggcct tgcctccttt ttctattttt ccctcataat ggtacataaa tggagatttt
    24781 taggcatatt tttggtataa taatacccat aaaagagcaa gtaaatgaat agtaggtgga
    24841 gatggaaaag tatttatcgg taacaacttt gaccaaatat ctgaaaatga aattcgataa
    24901 agacccatac ttggaacggg tctatttaac tggtcaagtt tccaactttc gtaaacgacc
    24961 tactcaccaa tatttctccc taaaggatga ccatgcagtt attcaagcga ccatctggtc
    25021 tgggatttat cagaaattag ggtttgacct ggaagaagga atgaagatca atgtgattgg
    25081 gcgtgtacag gtctatgaac caagtggtag ctactccatc atcattgaaa aagctgagcc
    25141 tgatggggtt ggggcgcttg cgattcagtt tgaacaactt aagaaaaaat tgacagaaga
    25201 aggcctgttt caagaacgct tcaagcaagc tctaccccaa ttttctaaga gaattggggt
    25261 agtaaccagc cgtagtggag ccgttattcg agatattatc acgaccgtca gcaggcgatt
    25321 cccaggtgtt gacatccttc tttatcctac caaggttcaa ggtgaagggg ctgcggagga
    25381 aattgctaga aatattgcgc gtgctaatca acgggacgat ttggatttgc tcattattgg
    25441 tcgtggaggt ggttctattg aggatctctg ggcctttaac gaagaaattg tggtacgagc
    25501 tatttttgaa tctcgtttgc cagttatctc tagtgtgggg catgagacgg atgtgacctt
    25561 ggcagatttt gtggcagatc gacgcgctgc aacgccaaca gcggcggctg aactggcaac
    25621 acctgtgacc aagttggatg tattagctca tttgcaaaat caggaaaaac ggatggcaac
    25681 ggcagtccga aatgttctat ctaagaaaca agaggctttg aaaaaatgca gtcagtctgt
    25741 tatctttaga caacctgagc gcttgtatga cggttatttg caacgcttgg accaactgca
    25801 actgcgtttg aaacaaagtt tgcgaactcg gatttctgat aacaaacaat tagttcaagc
    25861 aagaactcat caattagtac aattatcacc tgttaccaaa atccaacgct atcaagaccg
    25921 tttaggacag ttggacaagc tcttaggtag ccaaatggcg ttagtttatg acgccaaggt
    25981 tgctgaggcc aagcgacttt cggaagcttt gctcatgttg gatactagcc gaatcgtggc
    26041 gcgtggttat gctattgtca aaaaagaaga gtccgttgta gattcggttg agagtttgaa
    26101 gaaaaaagac caagtaacgc ttttgatgcg agatggtcaa gtagaattag aggttaaaga
    26161 tgtcaaaaca aaagaaattt gaggaaaatc tagcagaact ggaaaccatt gtccaaagtt
    26221 tggaaaatgg tgaaattgct ctggaagatg cgattactgc ctttcaaaag ggcatggtct
    26281 tgtcaaaaga gctccaagct acgctggaca aggctgaaaa gaccttggtc aaggtcatgc
    26341 aagaagacgg aacagaaagt gattttgaat gaaaaagcaa gaaaaattag ctcttgtcga
    26401 gtcggctttg gaagattttt atggagacca gcagtttgcc tctagtttac gggagtctgt
    26461 tctctattct attcatgctg gtggcaagcg tattcggcct tttctcttgt tagaagttct
    26521 ggaatccttg caggttacca tcaaacctgc tcacgcgcag gtagctactg ccttggagat
    26581 gattcataca gggagcttga ttcacgatga ccttcctgct atggatgatg acgattatcg
    26641 aagagggcgg ttaaccaatc acaagaaatt cggtgaagct atggccattt tggctggaga
    26701 tgccttattc ttagacccat atgccttgat tgcgcaggca gatttgccaa gtcagatcaa
    26761 ggtggacttg attgccaact tatcccttgc ttcaggtagt ctgggtatgg tggcagggca
    26821 agttttggat atggagggcg aacaccagca cttgtctctg gaagaacttc agactattca
    26881 tgccaataag actgggaagt tactagccta tcccttccaa gcggcagcta ttatagctga
    26941 attgtcacct gaaatgcagg tgaagctgaa aactgtgggt gaattgattg gacttgcttt
    27001 tcaagtcaga gatgatgtac tggatgtgac agctagtttt gaggaaatcg gcaagacacc
    27061 tcaaaaggat ctgcaggcag aaaaatcaac ctatcctgcc ttgttgggct tggaagagtc
    27121 cattgccttt tgtaaccaaa ccctggatca agctaatgaa aaattagaag aaattgccca
    27181 gcagcttccc tttgaaacag aatcgattgt aagtgtagta gaaagtttga gaatcaatgg
    27241 ctaaggaaag agtggatgta ctagcttata aacaggggtt gtttgaaacg agagagcagg
    27301 ccaagcgagg tgtgatggct ggtctagtcg tagcagtcct taatggagaa cggtttgaca
    27361 agccaggaga gaaaattcca gatgacaccg aattaaaact caagggggag aaactcaagt
    27421 atgtcagccg tggtggtttg aaactggaaa aggccttgca ggtctttgat ttgtcggtgg
    27481 atggcgcgac tacgattgat atcggggcct ctactggagg ttttaccgat gtcatgctac
    27541 agaatagtgc caagttggtc tttgcagtcg atgttggtac caatcagttg gcttggaaat
    27601 tacgccaaga cccacgagtt gtcagcatgg agcagttcaa ttttcgctat gctgaaaaga
    27661 ctgatttcga gcaggagccg agctttgcca gtattgatgt gagtttcatt tcccttagtc
    27721 tgattttgcc agccttgcac agtgtcttgg ctgatcaagg tcaggtggta gcacttgtca
    27781 aacctcagtt tgaggcagga cgtgagcaga ttgggaaaaa tggaattatt cgagatgcta
    27841 agattcatca gaatgtcctt gaatctgtaa cagctatggc agtagaggca ggtttttcag
    27901 tccttggctt ggacttttct cccatccaag gtggacatgg aaatattgaa tttttagcgt
    27961 atttgaaaaa agaaaagtca gcaagcaatc agattcttgc tgagattaaa gaagcagtag
    28021 agagggcgca tagtcaattt aaaaatgaat aaaaaagaaa gacttgaaaa aattagaaga
    28081 ttggtgacag attatcaaat cggcacgcaa gaagaaattg tagaacattt gaaagaagca
    28141 ggtatcactg ccactcaggc gacggtatcc cgagatatca aagagttagg tattgtcaaa
    28201 attcctttga gagacaacac ctatgtctac gaattgccaa aatcaatcgt aaaaagtctg
    28261 caactggctg aagacaatat cgaatcggct gaattgatgg ataagatgat caatctccaa
    28321 gttattccag gaaatacggc ttttgtaaaa gctcagttaa tcgagacttt tgcagacaag
    28381 atttttagct gtttgactga tgatagctcg attttagtca ttgccagaag tggaagtcta
    28441 gcagaggaaa tctttgaaca agtaaaaaat tggtaggtct atatgttact tgaaatttcg
    28501 ataaaaaact ttgccattat tgaggctatt tccctcaatt ttgaaaaggg gatgactgtc
    28561 ctgactggtg aaacgggtgc agggaagtcg attatcattg atgccatgaa tatgatgttg
    28621 ggagctcgtg cgacgacaga tgttattcgt catggtgcac caaaggcaga gattgagggg
    28681 cttttctcag tggagaatag tcgtctttta caggaaattt ttgatgagca aggtttagaa
    28741 ttaggtgatg aaattatcat ccgtcgggaa atcttgcaaa atggtcgtag tatcagccgt
    28801 gtaaatggtc agatggttaa tctgtctgtt ttgcgaacta ttgggcaaca tcttgtagat
    28861 attcatggtc agcatgacca tgaggagtta atgcgtcccc aactgcatat ccagatgttg
    28921 gatgaatttg gtgatgccgc tttttgggac ttgaaagaaa cctatcaaac gagttttgat
    28981 gcctatcgga aaatgcgcaa gcaggttctg gaagtcaaga aaaaccaaca ggaacacaag
    29041 gcacgtatcg aaatgttgga atttcaaatg gcagagattg aggcagcaaa cttgcaggct
    29101 ggagaagact tggatctcaa tcaagagcga gataaactcc tcaaccacaa aaatattgcg
    29161 gatacactga ccaatgccta cagtatgttg gacaatgaag atttttcaag tctggccaat
    29221 gttcgttcag ccatgaatga catggaaagt gtcgaagagt atgaccctga ataccgtgaa
    29281 atttcaagct ctctgtctga gacctactat gttttagaag acattagcaa acgtttggaa
    29341 gctatcattg aggaccttga ttttgatggc aatcgcctca tgcaggttga gaatcgtttg
    29401 gacctccttc atactattac tcgtaagtat ggtgggactg ttgatgatgt tttgctttat
    29461 tttgccaaga ttacggaaga atacaatctc ttgacaggca ataatctttc gtctgaggac
    29521 atggaagcag agcttaagaa gttggaagtc aatcttgtca atttggcagg tcaacttgct
    29581 tctgctcgtc ataatttggc tcagcaactc gaagctgaga ttaaacaaga actgcaagat
    29641 ctttatatgg aaaaagccca gtttcaggtt cgttttagta agggaaaatt cagtcgtgag
    29701 ggaaataaaa tggttgagtt ttatatttca accaaccctg gagaagactt taaacccttg
    29761 gttaaggttg cttctggagg ggaattatct cgtctcatgt tagccattaa gtctgccttt
    29821 tcacgtaaag aaggcaagac tagcattgtc tttgatgagg tggatacggg agtttcaggt
    29881 cgtgttgctc aagctattgc tcagaaaatt cataaaattg gtcagcatgg tcaggttttg
    29941 gctatctccc atttgccaca agtaattgcg attgcagatt atcaattctt tattgagaag
    30001 attagtaatg accattcaac ggtttcgact gttcgtctct tgacggtcga agagcgagtg
    30061 gaggaagttg ccaagatgtt ggcaggtgat gatgtgacag aagcagccct gacgcaagca
    30121 agagaattgt tgagaaacag ggagaaataa gatgacagat tattatgtaa ttggagatgt
    30181 tcacggaaaa gctgggatgc tggaagacct tctcaaaacc tgggatggtc agccccagtt
    30241 gctctttcta ggtgatttga ttgacagagg tgaggatagt caccgtgttc ttgaaatggt
    30301 caaggacttg gtggacaatc aaggggctat ctgtttgtcc ggaaaccacg agtatatgtt
    30361 tttgacttgg ctagatgacc cagaagaaag ttatgaccat tatagtcgca atggtggaga
    30421 tacaaccatt aactctatcc taggtcgtcc cttggatgca ccagttgatg gagtagaaga
    30481 tgccaagcgg gttgccgctg aagcggcaga cttggtcgaa ttcattcgtc aaatgccatt
    30541 tgtagtagag acagacaagt atatctttgt tcacgcaggt attgatttga ccttggacga
    30601 ctggcatgaa acaacagatt ataaaaaagt atggcttaga aaaccattcc acggagctga
    30661 aaatcatact ggaaaaacca ttgtcttcgg tcatacaccg gtctatggtt tgttacagca
    30721 agaacgcggt acatctgagc tttggactac agaagatggc aagattggaa tggatggagg
    30781 agctgtttat ggtggtgtcc ttcacgggat tgtctttaca gaccaaggaa tgacagaaca
    30841 ccactttatc gaaaatgacg gctttgtcgc tgaagattag tactcctagc agggtatggt
    30901 cttgtcaaaa tatcaaaaac aatttataat aaatagatac cctgaaagga agagaatcat
    30961 gaacttagaa gaattgaaaa aacgacagga gaagatccgt aacttctcta ttatcgccca
    31021 tattgaccac ggaaaatcaa ctctagcaga ccgcattttg gaaaaaacag agacggtttc
    31081 aagtcgtgaa atgcaggccc agcttttgga tagcatggag ctagagcggg aacgtggaat
    31141 tactattaag ttgaatgcca tcgagttgaa ttacactgca aaagatgggg aaacttatat
    31201 tttccacttg attgacacac cagggcacgt tgactttacc tatgaagttt cacgttcgct
    31261 agctgcctgt gagggtgcta ttttggtggt cgatgcggct caaggaattg aggctcaaac
    31321 tcttgccaac gtttatctgg ctttggataa tgatttggaa atcatgccta tcattaataa
    31381 aattgacctg ccggctgcag atccggagcg cgtgcgtaca gagattgaag atgtgattgg
    31441 tttggatgcc agtgaagcag ttttggcttc tgccaaggct ggtattggga ttgaagaaat
    31501 cctcgagcaa attgtagaaa aagtaccagc accaacgggt gatgtgacgg cgccacttaa
    31561 ggccttgatt ttcgactctg tttacgatgc ttaccgtggg gttatcctcc aagtgcgtgt
    31621 catggacgga gtggtcaaac ctggtgataa gattcagctc atgagcaata gtaagacctt
    31681 tgatgtggcc gaagtcggta tttttacacc aaaagcggtt ggtcgtgatt tccttgcgac
    31741 tggtgatgtt ggttacattg cggcgtctat caagacggtt caggatactc gtgtgggtga
    31801 taccgttacc ttggcaacca atcctgcggc agaaccatta catggttata agcagatgaa
    31861 tcctatggtc tttgcgggtc tctacccaat cgaatcaaac aagtacaatg acctgcgtga
    31921 agcgcttgaa aaattgcaac tgaatgatgc tagtcttcag tttgaaccag aaacatctca
    31981 ggcacttgga tttggtttcc gttgtggatt tcttggactt ctccatatgg atgttatcca
    32041 ggaacgttta gagcgtgagt tcaatattga cctcatcatg acagctccgt ctgttattta
    32101 caaagttaat ttgaccgacg gtgagtctat ggatgtgtct aacccatctg agtttccaga
    32161 cccaactaag attgcgacca ttgaagagcc ttatgtcaag gcgcaaatca tggtaccaca
    32221 ggagttcgtc ggagcagtaa tggagctagc tcagcgtaag cgtggggact ttgtgactat
    32281 ggactatatt gatgataacc gtgtcaatgt tatctatcaa attcctcttg ctgaaattgt
    32341 ctttgacttc tttgataaac ttaagtcttc gacacgtggt tatgcaagct ttgactacga
    32401 attgtcagaa tatcgcccat ctaagctggt gaaaatggat attcttctca atggagataa
    32461 gatggatgcc ctcagcttta tcgttcacaa ggactttgcc tacgaacgtg ggaaactcat
    32521 cgttgataaa ctcaagaaaa tcatccctcg ccaacaattt gaagttccaa tccaagcggc
    32581 tattggacac aagattgtcg ctcgtactga tatcaaggcc cttcgtaaga acgtacttgc
    32641 taaatgttat ggtggtgacg tttctcgtaa gcgtaaactc cttgaaaaac aaaaagctgg
    32701 taagaaacgc atgaaatcca tcggatcagt tgaagttccg caagaagcct tcctcagcgt
    32761 cttgagtatg gatgaagaat aagttatcag aaatgccttt ccttgttcac aggaaattta
    32821 tataagaatc aatagtaaag caggcttaga tggtctgctt ttttagtttt tatactcttc
    32881 gaaaatctct tcaaaccacg tcagcgtcgc cttaccgta
//
LOCUS       DAVBGY010000018        31623 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVBGY010000018 DAVBGY010000000
VERSION     DAVBGY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31623
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.018"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(300..518)
                     /locus_tag="VQ620_000878"
     CDS             complement(300..518)
                     /locus_tag="VQ620_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2763534.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(764..1042)
                     /locus_tag="VQ620_000879"
     CDS             complement(764..1042)
                     /locus_tag="VQ620_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763535.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEMLELACWFRVIIVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYSIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            complement(1256..2887)
                     /gene="rlmD"
                     /locus_tag="VQ620_000880"
     CDS             complement(1256..2887)
                     /gene="rlmD"
                     /locus_tag="VQ620_000880"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET2763536.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSLHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWCTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSQKNASLNKITNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2961..3935)
                     /locus_tag="VQ620_000881"
     CDS             complement(2961..3935)
                     /locus_tag="VQ620_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HET2763537.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(4009..4623)
                     /gene="pmp23"
                     /locus_tag="VQ620_000882"
     CDS             complement(4009..4623)
                     /gene="pmp23"
                     /locus_tag="VQ620_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HET2763538.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(4623..5597)
                     /locus_tag="VQ620_000883"
     CDS             complement(4623..5597)
                     /locus_tag="VQ620_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HET2763539.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQKENGTYSILIKNIEDIQSK"
     gene            complement(5605..6861)
                     /locus_tag="VQ620_000884"
     CDS             complement(5605..6861)
                     /locus_tag="VQ620_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET2763540.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMANVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6923..7354)
                     /locus_tag="VQ620_000885"
     CDS             complement(6923..7354)
                     /locus_tag="VQ620_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2763541.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(7356..7958)
                     /locus_tag="VQ620_000886"
     CDS             complement(7356..7958)
                     /locus_tag="VQ620_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HET2763542.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7942..8781)
                     /gene="prmC"
                     /locus_tag="VQ620_000887"
     CDS             complement(7942..8781)
                     /gene="prmC"
                     /locus_tag="VQ620_000887"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET2763543.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVENIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(8781..9860)
                     /gene="prfA"
                     /locus_tag="VQ620_000888"
     CDS             complement(8781..9860)
                     /gene="prfA"
                     /locus_tag="VQ620_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET2763544.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9857..10447)
                     /locus_tag="VQ620_000889"
     CDS             complement(9857..10447)
                     /locus_tag="VQ620_000889"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET2763545.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRGLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            10570..10752
                     /locus_tag="VQ620_000890"
     CDS             10570..10752
                     /locus_tag="VQ620_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HET2763546.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10889..12262
                     /gene="mnmE"
                     /locus_tag="VQ620_000891"
     CDS             10889..12262
                     /gene="mnmE"
                     /locus_tag="VQ620_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET2763547.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEANLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLIEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(12309..13244)
                     /gene="dapA"
                     /locus_tag="VQ620_000892"
     CDS             complement(12309..13244)
                     /gene="dapA"
                     /locus_tag="VQ620_000892"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET2763548.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(13300..14376)
                     /locus_tag="VQ620_000893"
     CDS             complement(13300..14376)
                     /locus_tag="VQ620_000893"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET2763549.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14718..14790)
                     /locus_tag="VQ620_000894"
     tRNA            complement(14718..14790)
                     /locus_tag="VQ620_000894"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14755..14757),aa:Thr,seq:ggt)
     gene            complement(14890..15399)
                     /locus_tag="VQ620_000895"
     CDS             complement(14890..15399)
                     /locus_tag="VQ620_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HET2763550.1"
                     /translation="MKKLTIRDIADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15397..15493)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15522..15953
                     /locus_tag="VQ620_000896"
     CDS             15522..15953
                     /locus_tag="VQ620_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET2763551.1"
                     /translation="MKIPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKAYIFRK"
     gene            16035..16412
                     /gene="mscL"
                     /locus_tag="VQ620_000897"
     CDS             16035..16412
                     /gene="mscL"
                     /locus_tag="VQ620_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET2763552.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16454..17548)
                     /gene="hemH"
                     /locus_tag="VQ620_000898"
     CDS             complement(16454..17548)
                     /gene="hemH"
                     /locus_tag="VQ620_000898"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET2763553.1"
                     /translation="MKKAILMMTFGSPEEISFEGVAEFFTNIRRGVRPQDHEIQTLYD
                     NYIRIGGTPLQRITREEVNLVKERLGEEYGIYFANKFSRPFIPDVIKQMETDGVEECI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEVLLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            complement(17626..18458)
                     /gene="srtG2"
                     /locus_tag="VQ620_000899"
                     /pseudo
     CDS             complement(17626..18458)
                     /gene="srtG2"
                     /locus_tag="VQ620_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218039.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG2"
     gene            complement(18536..19534)
                     /gene="srtG1"
                     /locus_tag="VQ620_000900"
     CDS             complement(18536..19534)
                     /gene="srtG1"
                     /locus_tag="VQ620_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG1"
                     /protein_id="HET2763554.1"
                     /translation="MKTKREKPKKSLSRRLVLAVDGVINHLLLIFAALIFLFGFYALW
                     DSNQVYSLASSSEYEAYRPVTTQQDELASFSGFSKLQELNPEVLGWINVYGTNIDYPL
                     VQAKDNEKYLNKDSKGEFAATGAIFLDARNNPKFEDFNTIIYGHHVENGVMFGDVAKF
                     ADQEFFDQHRYGSIYYNGVEKGLEIFEMLEVDAYDFNIYDPGIQGEDRQQAYLDHLLS
                     VAMHKRDISLSPSDRIILLSTCFLDVTNGRHIVVAKITDTVPKNTFHTKKSKPFPYSV
                     FDDSSLGRFLSSIPLWIWYLILFVLFLLLIFLLLVLYLILRRRRESKKNARSRPFY"
     gene            complement(19552..20748)
                     /gene="pitB"
                     /locus_tag="VQ620_000901"
     CDS             complement(19552..20748)
                     /gene="pitB"
                     /locus_tag="VQ620_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus major pilin PitB"
                     /protein_id="HET2763555.1"
                     /translation="MKKTFFKKLFTASIAAITALSVFRGVPTFADDNSAITKANGENN
                     AVVKINKTLNIAEGITTPTATFTFKFTEKTGQSSNGAPYQTGVAIPDRNVEYNKNDHP
                     TADKIQKATEDIFSGVAYGHAGEYVYDVAEAKTGWQAITKNGKTIDAMRYDKRTYEMH
                     VIVKNKVNGGVYISSVYFKENNKSNAPKVEPSEQGVYNLFDNTYTKDASKEPNPDDPS
                     QVDPNAKALTITKKVDGASGDKTRDFQFHIKIQLPSTNKTAETPVTNIIVKHGSKSEV
                     LAVVTPADTVEYNFTLKDGETFTVEQLPAGSKYTVTETGVAGYTDSSIYTTNGAEQTS
                     QGQKNVDFTLTDILIGEKKNDNKVTNKIDDVTPTGLLIDNLPFILMIGLGLAGFVVLS
                     KKRREA"
     gene            complement(20793..21344)
                     /gene="sipA"
                     /locus_tag="VQ620_000902"
     CDS             complement(20793..21344)
                     /gene="sipA"
                     /locus_tag="VQ620_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system signal peptidase SipA"
                     /protein_id="HET2763556.1"
                     /translation="MLLKKKHKKPVTQVNRDKSPPSVWGDILYLVSKLLMVGFVLAIL
                     YFFVFGLLRYNDDGMKPALKDGDLVVYYRLDKRYSIGDLLVYSYKGKERVARVIATEG
                     STIDINENGLIINGSPQQEQDIYKETLLYKEGATFPMKVPAGQLFVLGDNRTTAVDSR
                     AFGTIPIQDTQGKVVTVIRRRGF"
     gene            complement(21344..23296)
                     /locus_tag="VQ620_000903"
     CDS             complement(21344..23296)
                     /locus_tag="VQ620_000903"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET2763557.1"
                     /translation="MDAGIGTGTNINAGLTEAQRLLQSARAGAKKVVILLSDGEANMY
                     YESNSGRTIYNYYSNPNVGRMIDTPYWFTSGLERGMLNISSLIAPKIDGFYSIKFRYI
                     GSNDSITSLKGYISGYNSGIPNEIFSANNENDLQQKFKEITDKILPLGVHHVTISDVL
                     SKYVQLLPGDASHLRVVKIKDGNEQELNDNQVTIETKKNEQGLVEVTAKFNPSYTLED
                     DAKYVLKFTVTSSQEAFDAIAGDKTLTSDDAEEADATKLYSNKGAKVAYSYGIGTSRT
                     KIKDYSEKPTFKPSDPLTVPVEIEWKGVDGKSNPSANRPPSVELNLNQKKDGSIKDSY
                     RKVTSPVQTNSFTENTSFAKVAKGYDYELKAPDAPGYTVEVQKTGTKEKPSFKVIYRQ
                     LPSLTVKKILEGEQSPNKSFTINVTFSDKDGKPINGKFGNTTVTNGKAQISLKNSQET
                     ALSYLPRDTHYKVEEVENSRTGYHVTYEKQEGTLSEDVQTIVTNHRLPTLSVTKKVTG
                     AFANLLQSFKITINVKDAQNKPLNGSYSAIVNNQKTTLQFTNGKATVDLKKDKTIKIL
                     DLPLNARYSIEEEASSSRGYQVSYDKKEGTLDANKSATVTNNKNSVPETGIDFLSSTL
                     VLGVVLPLGGIFFIILLGHLVVNRRK"
     gene            complement(23426..23929)
                     /locus_tag="VQ620_000904"
     CDS             complement(23426..23929)
                     /locus_tag="VQ620_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HET2763558.1"
                     /translation="MNVQYDFKKIQYFTSSLVIFLAILFLCAPINSLRADSITEPQTT
                     LHKTITPISGQKDQYELSLDITSKLGTETQSEPLDVVLVADLSGSMEERDVWSYSSRR
                     YISRIEALKHTLKGVNGRQGLIDTILSNSQNRLSIVGFAGKIDNQYNDRYYNEYYLSY
                     QYGTWPN"
     gene            24288..25511
                     /gene="pepT"
                     /locus_tag="VQ620_000905"
     CDS             24288..25511
                     /gene="pepT"
                     /locus_tag="VQ620_000905"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HET2763559.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPRQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(25547..26176)
                     /locus_tag="VQ620_000906"
     CDS             complement(25547..26176)
                     /locus_tag="VQ620_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763560.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSANSKDKTALLESTKAILVLNHQIQSKVSASEETSPARNAEANSDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            26141..26362
                     /locus_tag="VQ620_000907"
     CDS             26141..26362
                     /locus_tag="VQ620_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763561.1"
                     /translation="MDLFQELICLVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(26633..26981)
                     /locus_tag="VQ620_000908"
                     /pseudo
     CDS             complement(26633..26981)
                     /locus_tag="VQ620_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(27086..27646)
                     /locus_tag="VQ620_000909"
                     /pseudo
     CDS             complement(27086..27646)
                     /locus_tag="VQ620_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(27872..30991)
                     /gene="phtE"
                     /locus_tag="VQ620_000910"
     CDS             complement(27872..30991)
                     /gene="phtE"
                     /locus_tag="VQ620_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HET2763562.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQISQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
     gene            complement(31201..>31623)
                     /locus_tag="VQ620_000911"
     CDS             complement(31201..>31623)
                     /locus_tag="VQ620_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2763563.1"
                     /translation="KPNEEKPQTEKPEEDKEHDEVSEPTHPESDEKENHAGLNPSADN
                     LYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIAEAEALLEKVTDSSIRQNAVET
                     LTGLKSSLLLGTKDNNTISAEVDSLLALLKESQPTPIQ"
BASE COUNT         8994 a         6480 c         5780 g        10369 t
ORIGIN      
        1 aaaaaaggat ctagttttta tttttagtta aattatgtct atatttttca ttaactcatc
       61 aataaaaatt tgtttgctaa tatctctttc ttctgatttt gtttcaagga ttattgctac
      121 atcattcata tttttaggta aataccaacc atctggctta tctgaatatc ccttgaagcc
      181 taattgatta aaagtagtta tttgaccagt accttgattg atgttttctt caacttcatt
      241 aaagcctata cttctcttgc ataatcccta acttgatctt ctgtccttaa agtcattttc
      301 tattcctcgt cactaaaatt atcttcaaaa ctaataatat cattaggagt gcaagataaa
      361 gccttacata ttctttctaa attcttaaat gtaattggtt tatcctttcc catttgtgcc
      421 ataacatttg ttgtaagtcc tgccatagct attacatctg ttttctttag tccttttttt
      481 gctaactgta tccataatgg tttatagtta agtgccataa tatccctctt tttttcttca
      541 ttaaatttat ttaattatag cataaatatt gatttcattc aatcttacat tgatttagtg
      601 atttgtttta gagaatttgt tgtgttattc cgtccttatt ttgccataaa ttgttttaag
      661 tgggacctct tatccttacc atttttacct cctaattttg agtataaaaa agacgataga
      721 gtttttaatt tctatcgtct gagatttaca tattaaattt tcatcacaaa ataagactct
      781 ttaaagaatc tttaaattca ttggtatgct gcttacaata tccattatta aaatggatac
      841 tataagcagc acaccaacta tttatttttt gagttttaaa ctttggtaga agtcttgaac
      901 tccttatatt tacataacta tttgcctcta taactattat gacacgaaac caacaagcaa
      961 gttcaagcat ctccatatct ttattcaaat gaggactaag atattcacaa actttatcat
     1021 aaacctcttt atcttttttc ataaaagggg catacaactt agcaaattta tcctaaaatt
     1081 ttttgttgcc tttatcaatc atttcaacgc tcctttaaat ataaaaataa tcctatcaat
     1141 ctttatattg agcctatttt ctaattgata ccttgcaaga aaggaataat agctgttcct
     1201 ataccaagta ggaatgtaat aaatgcagaa agtcatatac tgtcattctt ttctattaaa
     1261 tcattttgaa gtgtctcaaa gcatccatga tggcttcttc tttttcaggt gtacactgtg
     1321 gaataatttg tttatccttt ttagacttgt tgtaatgttc tcgtaattct attccacatt
     1381 tcttttttat ctgtgcaata tataatgtcg aaacttttaa ttcaaattta ttccaaacat
     1441 attctttgat ttgagcatat gttgctttgc tctccgcact tgtcaaatcc atctcatcca
     1501 gctcaatttc aacacttatg tgcttatcga catcgagttt ggacaaaagt gctaccgtct
     1561 cgacgtgatg cgtttgagga aatagatcca ccggctggac tttcttcaat tcatatccca
     1621 attcttggta gagtttgata tcacgcgcca tggttgcgac attgcaggag atataggcga
     1681 tgcgatcggc tcctgtttgg gcgcttgctt tgataaagct ttctgtcaag cccttgcgtg
     1741 gaggatcaac caagataacg gttggttgaa taccttcctt gagccatttc ttcatggcat
     1801 tttcagccgt gtcacagaca tagtgggcat tagtaatctt gttcaaagaa gcattcttct
     1861 ggctattctc aaccgcttct ggaatcagtt caacaccgta gacttctttg acatgcttgg
     1921 cgactgataa accaatggtt ccaataccag aataagcatc aataaccacg tcatcttttt
     1981 ttaactctgc aaagtcaatg gctgtttgat agagtttctc cgccatttca gtattgactt
     2041 ggtaaaaggc tgggccagcg atttggaagt catttcccaa catctggtcc gtaatatagt
     2101 cttgaccata aagagtgcac cactccttac caaaaatcgc attggtattc tggtcgttga
     2161 tattttgcat gacagacaca atctctggga actgcttgat aacttgttca atcaattggt
     2221 caacacgaaa aacttttgga cgagttgtca ccaaaacgac catgatttgt cctgaatagt
     2281 gaccacgacg caccacaaga ttccgaatca atccagactg ttccttttcg tcataaggtt
     2341 ttaaatcaaa acgacggagc aggtctcgaa gagctactac gacttggtca atgacaggat
     2401 cctggataaa gaaatcttca aggggcatga ggttgtgcga attcttacgg aaaaatcctg
     2461 tttccaagac accattcact cgacgaacgg gcacctgcgc cttattgcga tacttgactg
     2521 gatgttccat accaagcgtt tcagcaactt ctacatctgc aattccagca atcttgtaga
     2581 gactgtcctt gacttgcttg gttttaaact tgagctgttc tggataagaa aggtgtccta
     2641 aatccgcgat tcctgaacgc aggtaagcca aatctagatc ttgattacgg tgtagtgact
     2701 ggacaaggta tttttcaact tttccaaagc caatcttttt attgaccttg aggacacgca
     2761 tgagaatttt ttcactcggt aaagcattct ctacaaaaaa gaccaaacca tctaccttgg
     2821 caactcctgc cccttcatgg gtcaaatcaa caatttcaac ttctacaata tcatttttct
     2881 ttaacattct tttctctttc tattggccga caaaaaagac ggcaacgtta cgattaccat
     2941 ccattatacc atgaaatttc ttaagaacca aaactcttga agaagttgac aagggcttgc
     3001 cagagggagc ttaagaagtt accaccgtcc tctagcgcct tatccgcatc aaagttaagg
     3061 ttgatatttt taaaactgtc gccagcttgt gatacgatgc tttgtttaag gtcatttagg
     3121 gttttagtga aatctgcatt gctgaggata tcactctttg agagattcaa ggcaaaattg
     3181 atgatgatat tgatctggtt tcctgttatg acctgatcaa gtttgtaatt ttttaaggta
     3241 tcttcaacaa tcttgcggac atcttcttct gtcagatttc ccttgctttc tttagctttg
     3301 gcgagtcctg acttgatatc agctagggca acgtttaatt tattagcatc atagcctgat
     3361 ttgtccttgt tttcagcatt gatatctgac aaagccttta gctcttcttg agccaaatct
     3421 ttattggctt gcggcacctt ggctccatta gcttctagcg aatagtagat cccagccaaa
     3481 gcactctcac ctgtaactgg aataggggct gctacagtga ttttggcatg ttccataccc
     3541 agcgttactg ctgcgtttcg gtacatatcc tgagtcacct tagtgatatt ttctggtgtt
     3601 tcaatcttga cctcaagtgg cgatttgtca cctagctttt gaatcttggc tgatgaatac
     3661 aactgtaagc tagagtcatt ggccacattc atgattttag aataaacatc aggtgtcatg
     3721 gtcttgagtt ctttggtatc tgttgaggca ttgtagccca gttttttaag agtttgattt
     3781 ttttggtctt cagataggga ggaacctagg acatattcag gttggacata ggtttcatcg
     3841 ataacttttt gaacatctgt tgctgcatgg acgctattca tagctgttac tgcccacaag
     3901 accgcagcac tggtcagaaa gagtttcttt ctcataggga atttcctcct ttacttttct
     3961 agagtaatat atctatctta aagaaaactt ataacaaaaa cacctggtct agccagatgt
     4021 tgaaaagaga gtgaaacatt tgatgatgta aaggttaagt cgtacctgtc tagaataata
     4081 atagtttcct ccatttacat agagttcagc accgtgaaaa atggaaatgg ggtgaatata
     4141 actataagtc tttccagtcg tattaccaag caagggggca acagtctcac gagagtactg
     4201 tttggctaga gccaaggtat tttccttgcc attttgggcg ataaaatcga tataggcagg
     4261 tccaaaatta taggcttgaa cagctgtcca gatatctacc cccttcttct gcgccagata
     4321 gagattgcct gtcagagttt gaatgccttg ccgaatgcta gaggcattat cattgatggt
     4381 gttggttgaa ccacttgcag actcactaga ctgcataaca tcgccttctt ttccttttgt
     4441 ttcagtataa atcatagcaa gcacaagctc ttcgtttgct ggggtgtctt tttcactcaa
     4501 gatttctcgc accatgggtt gataggtcat gacttgtttg acatcttgat gaacgcggta
     4561 agctttatag ccagcaaaaa ggaagactgc tagtacaagc actcttcgaa ttcgtttaaa
     4621 cattatttac tttggatatc ctcgatattt ttgattaaga tagagtaggt tccattttcc
     4681 ttttggataa actcaacaga ctcggcgtct tgatagacgt tattgggaac gatgagctca
     4741 attccatttg ataaggagag tttttggttt tcaaatttct ttaattggcg actggcatca
     4801 atttcatcaa attgaacagg ttctggtacg gcttctttga cttggtcaat aaagctcaaa
     4861 cgagccgtca gattgttgtc aaaaaggtca ttagccaatt tctcaggtga caattcattg
     4921 ctttcttcta ggttgttgaa aatcgctgat ttgaccttgg attgaaattg aaaatcatct
     4981 gtgttaaaag attcagcaat tctctgggct gttttttcca gttccttgat agatttttta
     5041 ggagaaatct taggagcgac agcaagaaga ttatctgaaa aatagttcaa aaaagtcccg
     5101 ttgtacttga ttcgtttttc aatcaggtga tacttgcgac tctgaagatt gaccaccaag
     5161 gcctcgtcag cacccgttcc aaatccaggc aggttattct gagtcagctt gattggatta
     5221 tcaacttctc ctccgaggtg ggtcaaggtc tcccgcaggg caattcgcaa gaaagcgaaa
     5281 tgttctacac cttctttaga aaattgaaca aaaatcaagt cattggtctt gagattttca
     5341 gaaatgctaa actcctcttt ccagagatta gccagcgtta ctgatgtctc caacaaatcg
     5401 tctgtaatat gattgaagaa gggattttct tcttcgaaaa tcccagtctt ggcttcatct
     5461 gaatacacat gttcaatttt tttacgtagg tattcttcga tttttggagt aatattgaga
     5521 aacttatctg ctaagaacag ctcggtatca tccggactga actggtgaat aatggctttc
     5581 ttaatataaa tgtccataaa agttttattc ctcgtataat gggaaggcat ctgtcaattc
     5641 tttgactgca cttctcactt cttctaatac agcctcattt tctgaattct taagggtttt
     5701 aatgatgagt tcagccactt tgcgactttc ttcttcacca aatccacgtg cagtaatagc
     5761 tgctgctccg atacgaatcc cacttgtctt gaatggtgac aaggtttcgt aagggattga
     5821 atttttattt agggtaatat tgacttcatc cagcaagttt tgagcaactt ttccgttttc
     5881 tacaacctta gtcacatcaa caaggaagag atggttttca gttccacctg aaataatacg
     5941 gaaatcaggg tcttgcaaga agacatttgc catagccttg ctgttcttaa ttacattggc
     6001 agcatattcc ttgaaggctg gatccaaaac ttctttgaag gaaactgcct tagccgccac
     6061 aacatgctct aaaggaccgc cctgaatacc tgggaaaata gctgaattga tttttttagc
     6121 aagttcttcg tcattggtca aaatcaaacc accacgaggt ccacgaaggg ttttgtgggt
     6181 cgttgttgta gtgatatgag cgtatggcac tgggcttggg tgaagaccag ctgcaaccaa
     6241 gccagcgatg tgggccatat caaccataag cttcgcaccg acagcatctg cgatttcacg
     6301 gaattttgaa aagtcaataa tttgagaata ggctgaagca ccagctacaa tcagttttgg
     6361 ttttacttct tgggcttgtt tcaagatagc atcaaagtct aagagttccg tttcaggatc
     6421 aacactataa gaaacaaagt tgtaggtttg accagagaag ctaacaggag ccccatgagt
     6481 caaatgacca cctgatgcca aatccattcc cataaccgta tcacctggct caatcaagga
     6541 catgtaagcc gcacagttgg cctgacttcc tgagtgaggt tggacattgg caaatttagc
     6601 accgaaaatt tcttttgcgc gttcaatagc aagagtctct acaacgtcta ctacatcagt
     6661 tccaccataa taacggcgtc ctgggtaacc ctcggcatat ttatttgtca agatagaccc
     6721 ttgagctgcc ataacagcct tggaaactac gttttccgaa gcaattaact cgatattatt
     6781 ttgttggcgt tcttcttctt tggcaatagc attccagaga tcagcatcat atgctttaaa
     6841 atcatctttg tcaaaaatca taggtcttct cctttattgt gtgactagtc cattagtttg
     6901 attttacaat aagaaaatca aactaaaaga tgcgaataaa ccgtttctgc attttatcac
     6961 aagtatagcc aactttttca taaaatgcat gagcacccag acgatgattg gcagaattta
     7021 agcggataaa cccataacca catcttttgg cttcttgttc caacccttgt agtaaacttt
     7081 taccgatacc ttgaccttgc gcttgaggtg aaactgctaa agctaagata ttaaatcctg
     7141 ctttggaata gagtgattcg taaacttcag cgtggacata tccaagtaag acatgattag
     7201 ctgcatcctc atagccaagt aggaaatgat gggaatcctg agacagtcta gctagttggc
     7261 tagccgtttc ctctggacta aaagtataac ccaaagcctc ttggttgatg tcacatatcg
     7321 ctttcacatc tgtttcttgc aaatctctta gcatttcaag cctcctcaaa agaaatctct
     7381 ggcaaccgag caagaatatc ttctcgttta attgcgcctt ggcgtaagat tttcaccttg
     7441 tctccagaca aatccacaat agttgaatcc tgtccagtta gaaaagcatc gtcttccaga
     7501 cccagaacct cttggtcaaa atccttcaga atttgttcaa aggttacacc acttgcctga
     7561 cctgagatat tggcagacgg cccaatcaag ggacctgtct ctcgaattaa atccagtgtg
     7621 atagggtgac tgggcatccg aaatccaata gttgcaaggt cagaatttac ccaataggga
     7681 actcggtcat tggcttcgag aataatggtc aagggacctg gcaaaaaggt ctctacaagt
     7741 ttttgtagat aagttggctg attcttagaa aagtgcaaga tatcctcgaa agaggcgata
     7801 ttgagattga gtgccttatc tctaggacga cgtttgagtt ggtaaacatg gtcaactgct
     7861 ttttcatcta aggccttggc aaaaagacca taaacagtct ctgtaggtag aacgacagct
     7921 ccaccatttt ccaactcttg tctaatcctg tccatcatca accacaacca tcctattttg
     7981 accaaattgg tctttgagtg ttcgaacttt tttttcagga agatgttttc taaaaagttc
     8041 gggaacactt tgaccttgct tgtatccaat ttcaaggtaa atcttaccac catccttgag
     8101 atagtctttt gcatcttccg caattctaca gtaaatagct aggccatcct cgtctgcaaa
     8161 gagagctaga tgaggctccg aatgcaaaac attcaagcct acctctgact catcttcacg
     8221 agagatatag ggtggattgg atacaattat atcatatttt tcagaaattt ctgcaaaaca
     8281 atcagatttt ttaaaaaata tattaagatt ttgatcgcta gcattctctg atgcaagctc
     8341 taaggcctct tgagaaacat ctgctgctgt cactgaccaa tctggtctat tttttgctag
     8401 agcgagagca atagctccac tacctgttcc aatatccaga actgagagat tcgtctcaag
     8461 attttcagtt agaataagct ccactaactc ctctgtttct ggacgaggaa tcaaaacccg
     8521 ttcatccacc tttaaatgca ttccataaaa atctgcctgt ccaatgatgt actgagctgg
     8581 cttgtgagct gctagttgct ggtaaatatt ttctacaaat tgtttttctt cctctgttgt
     8641 cacctcctgc tggagggcaa aaataaagtc tgtaaaagat agatttttca gactacgata
     8701 gacaaaagag aggctttccg cttcctctcc ttgtcttatc aactcttctt caaaatttga
     8761 aaataattga gctaatttca ttatttgttt aattcttcta gtttttgtgt ttggtcataa
     8821 agcaccaagg catccacaac ttcgtccaat ttaccagaca aaatcgtatc tagtttttgg
     8881 agggtcaagc cgatacggtg gtctgtgaca cggttttgag ggaagttata agttcggatc
     8941 cgttctgaac ggtcaccagt accgattgtc gacttacgct cagcgtcttg ttcatcctga
     9001 gcaatctgag caaagtggtc agcgacgcgt gcacggataa tcttcatagc cttctcacgg
     9061 ttcttctgct gggtacgttc ttcctgcatc tcaaccttga tattggttgg caagtgaacg
     9121 atacgaacgg cagtcgcaac cttattgacg ttctgtccac cagcacctga tgcgtggtaa
     9181 atatcaacac gaagatcttt tggatcgata tcgtattcta cttcttcgac ttcaggcatg
     9241 acaagaactg tggcagttga ggtatgaaca cgaccttggc tttctgtcac ggggacacgc
     9301 tgcacacggt gggcacctga ttcgtactta agcttagagt atacagactg acctgaaacc
     9361 atagcaacca cttctttaaa cccaccgaca ccattcatag aggcttccat gacttcaaag
     9421 cgccagcctt gggcttccgc atacttttgg tacatagtta gcaaatctcc agcgaaaagt
     9481 gccgcttcgt ctccaccagc tgctccacgg atttcaagga tgatattctt gtcatcgttt
     9541 ggatcctttg gaaggagcaa aattttcagt ttttcttcat attcttcttt ttcagccttg
     9601 gcatctttga gttcttgctt ggccatttct tccaagtccg catctccgcc tgattcctta
     9661 atcatctctt cggcatcgac gatattttga aggacttgtt tatactcacg gtaggctatt
     9721 acggtatcac gattggaagc ttcttctttt gaaagctcca taaaacgctt ggtgtctgaa
     9781 acgacatcag ggtcactcag caattctcct agttcttcat aacggtcttc tacagcttgt
     9841 agttgatcat agatgttcat ttttcttcat tctccttatt gatttcaggg gcaaaataat
     9901 gtttacggca aaccgagata taggtttcat taccaccaat ttggatctgt tctccatcgt
     9961 aaacgggcag tccatcctgt gttcgcaaca ccatggtcgc ctttttcttg caatactgac
    10021 agatggtctt gatttcgtca atcttgtctg ctaaaagcaa gagatatttg gaaccttcga
    10081 acaattcatt gcgaaagtca tttttcaagc caaaagccat gacgggtatg tctaactcgt
    10141 ccacaacacg agctaggtca taaacatggt ggcgtttgag aaactgggct tcatcgacca
    10201 acacacagta aggtttttct ggtaggcctc ggatatagcc aaagatatcc gttgtttcct
    10261 caatcgcaag ggcagggcgt ttcatgccaa ttcgactcga cacatagcca acaccgtcac
    10321 gcgtatccag agctgaagtc ataatcacaa caccttttcc ttgctcctca tagttatagg
    10381 ccaccttgag aatctcgatt gtctttccag agttcatggt cccataacga taatacaact
    10441 gtgccatgtt tcttgcttca cgtccatttc taaatttttg ctacattcta gtatatcata
    10501 attttcttaa gctttaaacg gcaaaatgtg gtaaaatgga agaaatcaaa actagtggag
    10561 gaagctatta tgccatttgt acgcatcgat ttatttgaag gacgcacgct cgagcaaaag
    10621 aaagctcttg ctaaggaagt aacggaagca gttgtccgca acactggagc ccctcaatct
    10681 gctgtccatg tcatcatcaa cgacatgcca gaaggaactt acttcccaca aggggaaatg
    10741 cgtactaaat aagctagctt aagcagaatt gcttaggctt tttcaatctc caagtagcat
    10801 tcattgaaga aatatcctaa atttgttaca atttgaaaag aaacttggag aatttccaag
    10861 aaaagagcta ttaattaaag gaaacattat gattacacgt gaatttgata ccatcgctgc
    10921 tatctctact ccactaggtg aaggggctat tggtattgtc cgcctgagcg gaacagacag
    10981 ttttgctatt gcgcaaaaga tttttaaagg aaaagacttg aacaaggttg ccagccacac
    11041 tctcaactac ggtcacatta ttgatcctct gactggtaaa gtcatggacg aggttatggt
    11101 tggggctatg aagtctccaa agaccttcac tcgtgaggat attatcgaga ttaacaccca
    11161 cggtggtatt gcggtgacca atgagattct ccagctagct atccgtgaag gagctcggtt
    11221 ggcagaacct ggtgaattta ccaaacgtgc ctttttaaac ggtcgcgtag atttgacaca
    11281 ggccgaggcg gtgatggata tcatccgcgc caagacagac aaggccatga acatcgcggt
    11341 caaacaactt gatggttccc tttctgatct tatcaataat atccgtcaag aaatccttaa
    11401 cacacttgcc caagttgagg tcaatatcga ctatcctgag tatgacgatg ttgaggaagc
    11461 cactactgct gttgtccgag agaagacaat ggagtttgag caattactaa ccaagctcct
    11521 taggacagca cgtcgtggta aaatccttcg tgaaggaatt tcaacggcta tcattggacg
    11581 tcccaacgtt gggaaatcaa gccttctcaa caacctcttg cgtgaggaca aggctatcgt
    11641 aaccgatatc gctgggacaa cacgagatgt catcgaagag tacgtcaaca tcaatggtgt
    11701 acctctcaaa ttgattgata cagctggtat tcgtgaaacg gatgatatag tggaacaaat
    11761 cggtgttgag cgttcgaaaa aagccctcaa ggaagccaac ttggttctac tagtgctaaa
    11821 tgccagtgaa ccactgactg cgcaagacag acaacttctt gaaattagcc aagataccaa
    11881 tcgcattatt ctacttaata aaaccgacct gccagaaacg attgaaactt cgaaactacc
    11941 tgaagacgtt atccgtattt cagtccttaa aaaccaaaac atcgacaaga ttgaagagcg
    12001 aatcaacaac ctcttctttg aaaatgctgg cttgattgag caagatgcta cttacttgtc
    12061 aaacgcccgt cacatttccc tgattgaaaa agcagttgaa agcctacaag ccgttaatca
    12121 aggtcttgag ctggggatgc cagttgattt gcttcaagtt gacttgactc gtacttggga
    12181 aatcctcgga gaaatcactg gggatgctgc tccagatgaa ctcatcaccc aactctttag
    12241 ccaattctgt ttaggaaaat aagaaaaatc catgatcctt cattcggtca tggattttat
    12301 tgtctttatt agtaatctgg tcttaagacc cctgttacag ttgccttagt tgcttcgtag
    12361 tcgccatcta cgacaacctt gataatgcgt ttggcatctt cttctggtgc tggaacaaga
    12421 ggtagacgag tgggtccagc ttcaaatccc atatagttaa gaattgcctt aactggagca
    12481 ggacttggat aagagaagag agcattaacc ttaggaatga atttacgctg aattgctgcg
    12541 gctttcttca tatcgctttc tgcaatggca gtaaacatct cgtgcatttc atccccattt
    12601 gtatgagagg caacagaaat aaccccatcc gccccaaggt tcatggcatg gaaagcatct
    12661 ccatcctcac ctgtataaat caagaactct tcaggcttgt gctcaatcaa gtaagccata
    12721 ttagccaagc tagtacattc tttgacaccg ataatatttg gatggtcagc caagcgaagc
    12781 atggtttctg gagtcaattc gacaactaca cgccctggaa tgttatagat aataattggt
    12841 aggtcagaag catctgcaat agtcttaaag tgctgataca tcccttcttg agaaggtttg
    12901 ttgtagtaag gaacaatagc aagcccagct gcgaaaccac caaattccgc tacttctttg
    12961 acaaactcaa tagagtcacg cgtatcatta gtacctacac ccgcaatcaa aggaacgcgt
    13021 ccattgacaa ccttttgtac agccgcaaac aactccaact cctcatcgtg ggtcaaagtt
    13081 ggactctcag cagtcgttcc tgcgagaaga attccatccg tatgatgggc caataaatgc
    13141 tcaatcaagg ctggaatagc atcaaagtta atagaaccat cctcatgaaa gggggtaata
    13201 aaggctgtaa tgattttaca ttcttttaaa tcttgataag acatgaaaac acctctctat
    13261 ttcaaagaag gagttcatct gaactcctaa acgatatgac tattttaatt caaatttcaa
    13321 ttcggctgtt ggacgaacca atccacgttc atgaagagtt tcagcaatct gaactgagtt
    13381 ccaagcagca cctttgagaa ggttatctga aacaacccac atgtgaattc ctttttctgc
    13441 atccaagtct ttacggatac gaccaacaaa ggtatcacgc gaaccaactg cattgatagc
    13501 ttgaggatag atttgatgag ctacatcatc ttcaagaaca gcacctggga aggctgcgat
    13561 agctgctttt acttcttcga ttggagccac ttcttttgtt tcgatataaa cagactcaga
    13621 gtgagctgac aagactggaa tacgcacaca tgttgcagat actgcaatgc tatcatcttc
    13681 cataattttc ttagtttcct tggtcatctt catctcttcg tacgtgtaat cattatcagt
    13741 gaaaacatca atttgtggaa gagcgttaaa ggcgatagga taatgtttct tgtcaccacc
    13801 tgaaggcaaa atttccgcat gcaaatcaca tggtttcaca ccatcattca agacttcacg
    13861 aagttcacgt tgtgtctcaa gaattgctcc cataccagca cctgaaacgg cttgataagt
    13921 tgaaacaatg atacggtcca agccccattt ttggcgaacc ggctcaagag ccaccatcat
    13981 ttggattgtt gaacaattag ggcaggcaat gattccgttg tgggcatcaa gtgcatgagc
    14041 attgacctct ggaacaacca aaggaacatc tggattttga cggaaataag atgtattatc
    14101 cactactacc acgccagctt ttactgcgta tggtgcatac ttagctgatg tagaactacc
    14161 tgctgaaaag agagcaatat caactccttc aaaagctgtt tcagtcgttt cttcaattgt
    14221 aatatcttga tctttaaatt tcaatgactt acctgctgaa cgtgcagaag caaggtaacg
    14281 gattttatcg attggaagtg ttgattcttc caacattttt atcatctgag caccgacagc
    14341 acctgtcgcg ccgactacag caactgtata tcccataaat aacctctttc ggaattttct
    14401 aaaaatttct ataatagaag tattatacta ctttttccat gaattgcaaa gctttttcat
    14461 cattttttgg aaaataaaaa tcccagtcgc tagactaggg actaagaggc atcttcatgt
    14521 gatgagcagg ttcacacaac tcatcaaggt ccgctcctgc gttatgacct ccttatgctc
    14581 aatagtagtc cgaagactac ctatcgactc atcgcatatt ctatattact agagatactt
    14641 gattttgtca ataagaaatt ttttcgatcg agtataacaa aaaactctcg tattcaagag
    14701 tttccctata catatcatgc taattgccgg gatcgaaccg gcgacctcat ccttaccatg
    14761 gatgcgctct gccaactgag ctaaatcagc ttacttaaga agtatactat attcagaaaa
    14821 acttgtcaag tttccttaga aattttccat aggaaaaagc caggtaagaa ctacctagct
    14881 tatcttcttc tatttactta aatcaattcg acgaccaatt ttaccaataa taatcgcccc
    14941 aataatcaat gaagcaaatt caccgattcc tgttgtaaac caagtaagga agaatggtaa
    15001 ttctgctaca atatttaact ctgcagcgat agtaaacatg gaaattgaaa agaggattga
    15061 aaagaagaaa tgatcttttc gaatcaatcc gttaaagagg taatccttgc tatattttgc
    15121 aaaaagccaa acacctaaac tgaggaatac taaggttgaa ccaccaccaa caaagacatc
    15181 tagcagtccg aagctaaaga aattagcaat catacaaccg atggtaactc cgatgatgta
    15241 cttaggattg taaaaagcca taaagttcat catttctgaa atacgaaact gataagcacc
    15301 atagctgatg gcatttaggg gcggtgtgac agtcaaaacg acgtagatag cagcgacgat
    15361 tgcaatatct gcaatatcac gaatagttaa ttttttcatc ttttctcctt tggcggttgc
    15421 ccgcgtgtaa tatgcttggt gaaagaagct aagcaccaag ggttgcttta ttcaacccta
    15481 ctagtatagc acaatagcct gctttatgct atacttaaca catgaaaatt cctattcaaa
    15541 aattttttaa caatgaaatc ttagcctatc tcttttttgg ccttgcgaca actctagttt
    15601 cgattctctc acgactagtc atttatcaac taagtcatca ggaactcctg gctactgccc
    15661 tagcaaatat tatcggtatc ctatttgcct ttatcacaaa tgatacgatt gtatttaagc
    15721 aaaaaaagaa aaatagactt atccgtttag tgaaattttc tcttgctcgc ctttctactt
    15781 ttctgttaga cttgttttta acttttctct ttgtaaccca atttcctcac attatagggc
    15841 aattcgtaac tgaaaacatt gataagataa atgctattga aacaataatt gcacaaatat
    15901 tcattattat catcaattat atattgagca aggcctatat ttttagaaaa taatgctcca
    15961 tttatgagcc ttttaattgc cttctataat gatttaggat acaatgaaac acgaaacatt
    16021 gaaaggaaaa cagtatgtta aaaaatctaa aatcgttctt gcttcgagga aatgttattg
    16081 accttgctgt cggtgttgta attgcctctg cttttggtgc tatcgttact tcacttgtaa
    16141 acgacattat cactcctctt attttaaatc ccgctttgaa agctgctaaa gttgaacgta
    16201 tcgctcaact ttcttggcat ggagtcggct atggtaactt cttaagtgct attatcaatt
    16261 ttatctttgt gggtaccgcc ctcttcttta ttatcaaggg cattgaaaaa gcacagaaac
    16321 tgactggcat aaagaaagaa aaaactgccg aaaaaaaacc aaccgaattg gaagtccttc
    16381 aagaaataaa agctctcctt gagaaaaaat aatcgattaa aaggatctag cgcaaagcta
    16441 aatccttttt tctttactct ttgggtaact tgcctgcttt ttcaagcatt tttttgatca
    16501 gataaggcat cttaacattc tcccgccctt ttttctcaat cagttttttc acaaattctg
    16561 gcatttgtaa atcttcagat tcagctaaaa tgtttttcgt ctcgtctgaa ggaactaact
    16621 catcaaaggt ctcttcttct gggagaaatt ccttaaactc ttgattttca ttgactctaa
    16681 ctgtattgac caaagcatca atcaaacgag aatctgtatt tggcattggt ggacgatggt
    16741 agtttacccc aaattcctgg cataagtcat aacattctac atcattgtca aacaagacct
    16801 caatatgctc actgatgaaa ctaataggta caaaaatata atggtctgga tgttctgtct
    16861 gttctctgag atactccaaa acatctggtt taatccatgg aataccaata tcactctcac
    16921 tctgccaagt gttggtatat tgttctgaac tcaagcccag tttttcagcg actagcttgc
    16981 tattttcgaa aatttggtct atataaggat caccaaagtc caaggcaaaa atgggcacac
    17041 tatgggctga aaagatgact ttaaagctgt cttgctttac ttcttctttt aaaatcttag
    17101 caatttcatc tgcccaatag tttaagagca cttcttcctg ataccagtcc ttaatgacca
    17161 aaaattgaat ttgtttgctt tctaaaaatt tttcatagcc catgacagag tagaaagaat
    17221 aatggggctc caaaatcaag cagatacatt cttcaacacc atcagtttcc atctgcttga
    17281 tcacgtctgg aataaaggga cgagaaaatt tgttggcaaa gtagataccg tactcctccc
    17341 ctaaacgttc tttgactaaa ttgacctcct cacgtgtaat ccgctgcaaa ggcgtcccac
    17401 cgatacgaat atagttgtca tagagagtct ggatttcgtg gtcttggggt ctaactccac
    17461 gacgaatgtt tgtaaagaat tctgctactc cttcaaaact aatctcctct ggagatccaa
    17521 aggtcatcat taatatggct tttttcatgt tatcatcctt atgatattat tttctctatc
    17581 cattctatca tgaaagaaaa aaatagtaaa cgtttcctac gtctactatt tcttttgctc
    17641 ttttgttttt ttatctcgtc ttataattag gatgataagg acaagaagta agaggacgat
    17701 aataaggagt agaaaggcta gaatgtaaat ccaatgactg ggaattccgt gatcgaaata
    17761 ccgtgttcct actgcttccc cactttcatc ctgtgctact ttcaccggtg catccgttat
    17821 tttagctaaa aggatatgtc gtccattagt tatgttaagg aaacatctcg cttgtacttg
    17881 gctttactta gcagatattg atagtaagcc tggtgttcct catccttgga actcaaggtt
    17941 cgataaatct ccgtgtcata ggcatccact tctaaaatcc caaaaatttc aagaccacgt
    18001 tctcgaccat tgtagtagat agaaccgtag cgatgctggt cgaagaattc cttatcagca
    18061 aatttcttaa tctcaccgaa catagcccct gaagccatag agtgtccgta gatgatcgta
    18121 ttaaaatcag taaagttggg attaaagcga tagtcgagaa aaattgatcc cattatcgta
    18181 taatcgccaa aggcattgcg gtcaaggtat ttgacattat cctgactttg tagaagcgga
    18241 tagtctacct tggtcccatc aaggctaatc caaccgcaaa catctttgtt caagtgctgt
    18301 agggtttcaa aacctgtgct cgaagcatcc ttttcaatag gattatagtg atcatagtca
    18361 cttgcttcta ctactctctg ccaactaaaa agagctacaa aaacacagaa gaataccaga
    18421 aatacgtttt taaatcgcgc tcttttttga accgtcatca gcactccttt ctatgcgaga
    18481 tttcttaaaa ggaaaggagg cgggacaaaa ccgcctcttt tctattcacc cttagtcagt
    18541 aaaagggtct gcttcttgca ttttttttac tctctcttct acgacgtagg atcaagtaga
    18601 ggacaaggag taagaaaatc aagagcaaga acaatacaaa caagataagg taccaaatcc
    18661 atagtgggat tgatgagagg aaacgtccaa gagacgagtc atcaaagaca ctgtatggaa
    18721 atggttttga tttttttgta tggaaagtat ttttagggac ggtgtctgta atctttgcga
    18781 ctacgatatg acgaccattg gtcacatcga gaaaacaggt actgagtagg atgatacgat
    18841 cactcggtga gagtgagata tcccgcttgt gcatggcgac tgagagcagg tggtctagat
    18901 aggcctgctg gcggtcctca ccctgtattc ctggatcata gatgttaaag tcataggcat
    18961 caacctccaa catctcaaag atctcgagcc ctttttccac accattgtag tatatactac
    19021 cgtaacgatg ctggtcaaaa aattcctgat cagcaaactt agccacatca ccaaacatga
    19081 ccccattttc tacgtggtgc ccgtagataa tggtattaaa gtcttcgaac ttaggattat
    19141 ttcgtgcatc gagaaagata gcgcctgtcg ctgcaaactc acctttggag tccttgttga
    19201 gatacttttc attgtccttg gcttggacta agggatagtc gatattggtg ccatagacat
    19261 tgatccaacc gaggacttcg ggattgagtt cttggagttt gctgaagcct gaaaaactgg
    19321 ccagctcatc ctgttgcgtc gtgacaggtc gataagcttc gtactcactt gacgaagcta
    19381 aggagtagac ttggttggaa tcccaaaggg cgtagaaacc aaagagaaag atcaaagctg
    19441 caaaaatgag cagcaagtga ttgatcaccc catccacagc aagaacgaga cgcctagaca
    19501 gactcttttt tggtttctca cgcttggttt tcatcatctg cccccagccg tttaggcttc
    19561 tctacgtttt ttagacaaga caacaaatcc agccaaacca agaccaatca tcaaaatgaa
    19621 tggaaggtta tcaatcaaga gaccagtagg agtaacgtcg tcgattttgt tagtaacttt
    19681 gttgtcgttt ttcttttcac ctatgaggat atctgttaat gtaaaatcta catttttttg
    19741 tccttgagat gtttgttctg caccatttgt agtataaatt gatgaatctg tataacctgc
    19801 tactccagtt tcagttactg tatatttaga acctgctggt agttgttcaa ctgtaaatgt
    19861 ttcaccatct ttaagagtaa aattgtactc aactgtatct gctggggtca ctactgccaa
    19921 cacctctgac ttagatccat gttttactat aatattcgta acaggggttt ctgctgtttt
    19981 atttgtactt ggaagttgaa tcttgatatg gaattggaaa tctcttgttt tatcccctga
    20041 agctccatca acttttttag taattgttaa tgctttcgca ttggggtcta cttgactcgg
    20101 atcatcagga ttaggctcct tacttgcgtc tttggtatat gtgttatcaa ataaattata
    20161 aacgccttgt tcacttggtt ctactttagg ggcgttagat ttattatttt ccttaaagta
    20221 tactgatgaa atatagacac caccatttac tttattctta acaataacgt gcatttcata
    20281 tgtacgtttg tcgtatctca tggcatcaat tgttttacca tttttggtaa tcgcctgcca
    20341 tccagttttt gcttccgcta catcataaac gtattcacca gcatggccat aagcaactcc
    20401 cgaaaaaatg tcttctgttg ctttttgaat cttatcagca gttgggtgat catttttatt
    20461 gtattctaca tttctatctg gaattgcaac tccggtttga tatggcgcac cgttagaaga
    20521 ttgtcctgtt ttttctgtaa acttaaatgt aaatgtcgct gttggtgttg ttattccctc
    20581 tgcaatattc aacgttttat taatcttcac aacagcatta ttttcaccat ttgctttggt
    20641 tattgctgaa ttatcatccg caaaagtcgg gacacctctg aatacggaca aagcggttat
    20701 agctgcaatg cttgcagtga atagcttttt aaagaatgtt tttttcatga ttatttcttt
    20761 tgttttttta ttttcttttt tcattatgat catcaaaagc ctcgtcttct aatgactgtt
    20821 acaactttgc cttgagtatc ctgtataggg atggttccaa aagcacgact gtctacagcc
    20881 gttgttcgat tgtccccgag gacaaaaagt tgtcctgctg ggactttcat cgggaaggtt
    20941 gccccttcct tatagagcag cgtttctttg tagatatctt gctcttgttg aggagaaccg
    21001 ttgatgatga gaccattttc gtttatatcg attgtacttc cttcggttgc tatgacacgc
    21061 gccactcttt ccttaccttt ataactatag actagcaaat caccaatcga atagcgttta
    21121 tccaacctat aatagacgac caagtcgcca tcttttaagg cgggcttcat gccatcgtca
    21181 ttgtatctta ataatccaaa gacgaaaaag taaaggatgg ctagtacaaa tccaaccatc
    21241 agaagtttac tgactaagta aaggatatct ccccagacac tcggcggaga cttatcccga
    21301 ttgacttgtg ttactggttt cttatgtttc tttttaagca gcatcacttc ctcctattca
    21361 ccacaaggtg accaagtaag atgataaaga agatccctcc tagaggaaga acgactccaa
    21421 gcacgagagt gctactcaag aagtcaattc ccgtttcagg tacgctgttt ttattattcg
    21481 tgactgtcgc agacttattt gcatcaagag ttccttcttt tttatcatag gacacctgat
    21541 acccacgaga cgaacttgct tcttcttcga tactataacg agcatttaga ggaaggtcga
    21601 gaatcttgat ggttttatct ttctttagat caactgtcgc cttaccattg gtgaattgta
    21661 gcgttgtttt ttgattattt actattgcac tatacgatcc attcaatggt ttattttgcg
    21721 catcctttac gttaatggta atcttaaagg attgcagaag attagcaaaa gcacctgtaa
    21781 ctttttttgt gactgaaagt gtcggaagtc tgtggttggt gacgattgtt tgaacatcct
    21841 ctgacaaagt cccctcttgt ttttcatagg tgacatgata tcccgttcta gagttctcta
    21901 cttcttccac cttatagtgg gtatcacgag gcagataact gagggcagtt tcctgactat
    21961 ttttgagaga aatctgtgct ttcccgttag tcactgttgt attcccaaac ttgccgttaa
    22021 tcggcttgcc atccttatct gaaaaggtaa cattaattgt gaaagattta ttaggtgatt
    22081 gttcaccttc taggattttc tttacggtga gacttggaag ctgtcggtaa ataactttga
    22141 aggatggttt ctctttcgta cctgtctttt gaacttcgac tgtgtatccc ggagcgtctg
    22201 gtgctttcag ttcgtagtca tatcccttag ctacctttgc aaaactagta ttttcagtaa
    22261 aactattcgt ttgaactgga ctagtgacct ttcgatagga atcctttata cttccatctt
    22321 tcttttggtt taagtttaat tcgacactag gtggacgatt tgctgatgga tttgattttc
    22381 catccacacc tttccactca atctctacag gaaccgtcaa tggatctgac ggcttgaaag
    22441 tgggcttctc agaatagtct tttattttgg tacgtgaggt cccaatacca taggaatagg
    22501 caacttttgc ccccttgttg gagtagagtt tagtagcatc ggcttcttcg gcatcatcac
    22561 tagtaagtgt cttatcaccc gcaatcgcat caaatgcctc ttggctagag gtgacagtaa
    22621 acttgagaac gtacttggcg tcatcctcca aagtgtaact cggattaaac ttggctgtta
    22681 cttccactaa tccctgttcg ttcttcttag tttcaatcgt aacttgattg tcattcagtt
    22741 cttgctcgtt accatccttg attttgacga cacgaaggtg tgaagcatca ccaggtaaca
    22801 gctgcacgta cttggacaag acatctgata tagttacatg gtgtacgcct agaggtagaa
    22861 ttttatctgt gatttctttg aatttttgtt gcaagtcatt ttcattattg gcagaaaata
    22921 tttcgttggg gattccagaa ttataaccac tgatatatcc tttaagagat gtgatactat
    22981 cgtttgaacc tatatatctg aatttgattg aataaaagcc atctattttt ggagctatta
    23041 aactagatat attcagcatt cctctctcta aaccagaggt aaaccaatat ggagtatcaa
    23101 tcatacgtcc cacatttgga ttagaataat agttatatat tgttctccca ctattagact
    23161 cgtaatacat attagcttcg ccatctgaca gcagaataac tacttttttt gccccagccc
    23221 ttgcactttg caacaatctt tgagcttcag ttaacccagc atttatattt gtcccagtgc
    23281 caatgcccgc gtccatacca taagaacgac tagagtcagc aattgttaac gaactaacaa
    23341 tattttttga gctattagaa tccgtgctcc aagatactaa agttttagca tcatcatatg
    23401 aagagatatt tgaataccaa ccagctcaat ttggccaagt tccatattga taactcagat
    23461 aatattcatt ataataacgg tcattatact gattatcaat ctttccggca aaaccaacta
    23521 tagacagacg gttttgggaa ttagaaagaa ttgtatcaat gagcccctga cgaccattca
    23581 cacctttcag tgtatgtttt agtgcttcaa tcctactaat gtatcgtcta ctagagtaag
    23641 accacacatc tcgctcttcc atactccctg aaagatcggc aaccaagact acatccaagg
    23701 gttctgactg ggtctccgtt cccagtttag atgtgatatc cagtgacaac tcatactggt
    23761 ctttttgccc tgatatcgga gtaatcgttt tgtgcagagt tgtctgaggt tcagttattg
    23821 aatctgcacg taaagaatta attggtgcac acaaaaaaag aatagcgaga aagataacta
    23881 aactactggt aaaatattga atcttcttaa aatcatattg aacgttcatg ctatctccct
    23941 tgctagaata tgttttaatc tgatactata taaatctgag acttccataa gataattgta
    24001 taataatatt aacttcgtgt caattaatgt gtttgaaaac atgttattat aaattacaaa
    24061 aaagtatgat tcttctttgt ttttcaactt tttctgtggt aaaatggaaa aaatagtaaa
    24121 aaatttcata aaataaagat gcatatattt attctaatcg ctcgaaaatt tcttttgaaa
    24181 gtcttctgaa tttgaattat tatatttttt caaaattcat aaaatttctc aaatcatatt
    24241 tttccgaagt tttgtattct attttatgat aaaaaaggag tttttttatg acttatccaa
    24301 atctcttgga ccgcttctta acctacgtta aggtcaacac gcgctctgat gaacactcta
    24361 ctactactcc aagtacacag agtcaggttg acttcgcaac aaatgtccta attcctgaaa
    24421 tgaaacgtgt tggactgcaa aatgtttact atctaccgaa tggttttgct attggaacct
    24481 tgccagccaa cgatccgtct ttaacacgta agattggttt tatatcgcac atggatactg
    24541 ctgattttaa tgctgaagga gtcaatccac aggtaattga aaactacgat ggtggtgtga
    24601 ttgaactagg gaattctggt ttcaaactcg atccagccga cttcaagagt cttgaaaaat
    24661 atccaagaca aacactcatc acaacagatg gaacaacctt gctaggtgct gatgacaagt
    24721 caggaattgc tgaaattatg acagccattg aatatctaac tgctcatcct gaaattaagc
    24781 actgtgagat tcgtgttggt tttggtccag atgaagaaat cggtgttggt gccaataaat
    24841 ttgatgcaga agattttgat gtggattttg cctacactgt tgatggtggt ccactaggtg
    24901 aacttcagta cgagactttc tcagccgctg gtgctgaatt gcatttccaa ggccgtaatg
    24961 tccaccctgg tactgccaaa gggcagatgg tcaatgccct tcagctagca attgattttc
    25021 ataatcaact tccagaaaat gaccgacctg agttaactga aggttaccaa ggtttttacc
    25081 atctaatgga tgtgacaggt agtgttgagg aggtgcgtgc aagctacatc attcgtgatt
    25141 ttgaaaaaga tgcctttgaa gcgcgtaaag catccatgca atctatcgct gataagatga
    25201 atgaagaact tgggagcgac cgtgtcactc tcaacttgac agaccagtac tacaatatga
    25261 aagaagtcat tgaaaaagat atgactccaa ttaccattgc taaagccgtt atggaagatc
    25321 taggtatcac gcctattatc gaaccaatcc ggggtggaac agacggctct aagatttcct
    25381 ttatgggaat cccaactccg aatatctttg caggtggcga aaatatgcac ggacgttttg
    25441 aatacgttag ccttcagact atggaacgtg cagttgatac catcattggc atcgtagctt
    25501 ataaaggcta aaaagacgag gtggctcagc tacttcgcct ttctttttat tctactggtt
    25561 tttcttgatt tccagtagtt gtagaagatt ctgttgtttc attttctgaa gttgattcag
    25621 caggtttaga atctcttgta ttgcttggtt tgttttcgtc gctagcagtt tcaatgttag
    25681 attctgcagt tgcgtttggt tggttctcag cactggtgtt atcactattt gcttcagcat
    25741 ttcttgctgg acttgtttct tcacttgcgc taacttttga ctggatttga tgattcaaaa
    25801 ctagaatagc ttttgtcgat tcaagtaaag ctgttttgtc tttactatta gcagaaagtt
    25861 gatctaataa tgcatccacc ttatcaaagt ccgcatcaga tccattatta ctttctaaat
    25921 aagagtgaag cgacatgaga atatcgtaga gtttttgata gagtacaagt gtctgagaat
    25981 cttgctcagc attttccttt tcttgttgaa gggcgctagc gatacgagtc aaaacatctt
    26041 ttacctgact gtttacttca tccaagtctg catcagcctt gtttgtggca gcttttagat
    26101 tttctacttc ttctgccaag gattgtctga ttccttcttc atggatttgt tccaagagtt
    26161 gatttgcctt gttcaaaaga ctttctactt cttccttgct atctgtcgca gattattggt
    26221 tgctatctac catgtactcc taaaacagga gagttataat ccaagattac aaggccttac
    26281 agaaataaga aatccagata agacaatgtt cgtccaagac gctattcgct tcgcacagca
    26341 gcacggattc aatatgcttt aattttaaag tttaggtgtc aagacctctt tttagtgtgc
    26401 ccaaaattta gagaagtaat caatcaacta acttttattt ttttcaaact ttcagtaaac
    26461 tgacctaaag ctaactcaat ctgtctttgt agatgcttct gctatcagct agaagttgat
    26521 ctacttttgc caagactgcc ttctcatcaa aagttccagg ttgatagttg gattgcaggg
    26581 atggaatctt gtttttcaaa gccgcttcat atcccttagt ttgaaccttg atgtagtgat
    26641 tgtggtcgcc atgaggaatc acaaaacctt ctgaatcttc acttataatt cgattggcat
    26701 caaaaaccat aaccatcttc ttcctcatgg tggacatgta gtgacggatt acttaataca
    26761 gaactagaag aacttcctac ctcttccgtg ttagagtgtg atgggggatt gttaagagat
    26821 gacttaggaa tatagtgata gtgttcccca tgtcttacta tataagcatc acctatatct
    26881 ctgacaatat cattagggtt aaagacatat gtggctgcta attcacctgc cgacaagtca
    26941 ctctcaggaa tgaaatgata gtgaccacca tgtggtacta ttagtagatt gaaatagaat
    27001 atgagcaaat tgataagggg attttaaagt aatttctaac aatgatttag aaactatgat
    27061 gtgctattct aaattcaact cactatatat aaccatcatc ggtagtataa cgtccctgta
    27121 attttgctac agatacttct gcactagctc ctttatcgtc tttaccatgt tcttgttttt
    27181 ggcgattgat ttcatctttt gttcgtacat tttctgcatg agcttgatct ttaaggtaaa
    27241 cataatactt tccatctacc ttaataatat atcctccctt aacctaactg acaatatctt
    27301 gatctttcgg ctgatagttg ggggctttca ttaatggctc ttcactaaag agcgcatcaa
    27361 aaggaacttt accattatag tagtgataat gatcgccatg agaagttaca taaccttgat
    27421 ctgtaatctt aataacaatt tgttttgctt gaattccttc tttttgacta acctagtctg
    27481 gagtcaaatt ttcagtcttc ttagtgtctt tattactgtt tacatatgaa acacgatttt
    27541 tatctgtatt ggcctgttag ctatgttggt tcagagcata aacacacaga cttaaggaaa
    27601 ggataacaac agatccagct gctatatatt tctttttaaa tttcataatt acctcatttc
    27661 tataattatt tatatgatgt cttcattatt aaatgattat agaaattaat taaccaatta
    27721 attaactagt aaatattcca cctcttttta agttgtatgt caagaaattt tatatattaa
    27781 taataaaatg aaattctccc aaagtcagag ttttatttct aacttttgag agaacttcat
    27841 ttttgattca gactttttct actgctattc cttacgctat gagatcagat aaattctttt
    27901 ttatcacttc tccacttggc aatcttaatt caatcgttcc atccatattg aatataacac
    27961 tatctaagcc taatccgtaa ctagctgtaa atttttctaa tttttcttgt acaggatcta
    28021 ctgctggagc ttcctctaat gctggatcta acatagggtc actccccaca ttcccttctg
    28081 gattcaacat tccattatcc gttgagtttt ctggttttac aggtttttcg tttggtgcct
    28141 ctggtaaaga atctgctggt ttattttctg ttggttggtt ctcaactgtt ccagtagata
    28201 cttttccatt ttcagatggt ttattttcac catttccttg aggtgcttct cctgtaaaat
    28261 ctgccatatt ctttttaatg acttctcccg atggtaaata taattcaatt gttccgtcca
    28321 tattaaacaa gacattttct agcttcatcc cataactttc agcaaatttt gctacttttt
    28381 cttgtacagg atccactgta ggaacttctt ctaacgttga attactagta ctattcccag
    28441 tttcagaaag tttttctttt tctaccttct cactagtctt tggttcttct accttttcat
    28501 caagttttga gttttcttgt gctttattcc ttttaaattg tggtagaata cttggtttat
    28561 cagtttgatt ttctttttcc aagataggta cttccacaat ataagtcgat tgattgtcca
    28621 aataagcatt tgccatgaag gttacaggaa ttttatttcc ggccgttctg gttgttcctt
    28681 ggtttaattt cggaatcggt aatttgattt caccaacttt atagttattt tctaaataag
    28741 catttccatg aaattcatca aacactctga ctaaagcatc agttccttta ggcactgcaa
    28801 attgagggtt cactcttaaa taagtatccc ctgcatggaa aggatagaaa atcgtttgac
    28861 tggccatttt gtaagctaaa gaggttggaa ctgtaaatgt accatcataa cttacttctg
    28921 gataatcttt tgaagcgata gtatacttaa atgtttgtcc tggtaaataa ggttgatcta
    28981 attcaaagtt tgcaatattc cctactcctt ctccaaatac tttaccagat actttctcca
    29041 atacttttcc atctggtgtt attaatttta ctagcatatt gatacctaat tttttctcca
    29101 attcaggcgg aaaactaaaa gaaacgcgtt tttgaccatt ggctagagta aagttttgat
    29161 tattaaacgt actatttttt aacaaattaa caacattcgt taattcttct ccagtataaa
    29221 ctttattccc ttctttttta gcaactcctt cttcgggttt aaacagttca tagttactgt
    29281 gagaatgacc aattccaacc ggtttatgtt catcaatcgg atctgcatga tggtgatctc
    29341 catgcggata aataatcgca tttttttctt tattcacgac aatactttca cgtttgacac
    29401 catattgttt cataatgcca gcaatttttt cttcgatttt tttatctaaa tctttcattt
    29461 ctttggcatt acttggataa tcctgttcat gagatgacaa agaatctaat ccattatgac
    29521 tagttttaac ttcctctaaa tgtttttgcg cagccttaat ttgctcttct gtcaagtcct
    29581 tcttgaagaa ataatgattg tggtctccgt gactcatgac aaaacctgat tcatcttcag
    29641 cgataatacg attagcatca aatccgtatc catcttcttc atgtttctca tgtgaagttc
    29701 ctggattgat tggaagagat ggagaaggtg ttgctagact attgtttgga agagtcggtt
    29761 gcccaatttg atttgatttt ggaatgtaat ggaaatgatc accatgtctt acaatataag
    29821 ctgtagccgt ttcttcaacg atatcttttg gattaaaaat ataaccatca gatgctgaag
    29881 agagctcctt acttgtcgtt aaagaagaag gattgcttga aagactgcct agactagaca
    29941 ctacttcatt aggttttgca tttgtagaaa ctgtagaacc agttccactg ataggcacca
    30001 ttctggcaat cttttcttct aaggcagaaa gcttgctgta aggaataaag tggtaatggt
    30061 cgccatgcgg aatcgcaact ccatttggtg tacgactgat aatcttagca gggtcaaaga
    30121 ccaggccatc tgattcactg taacgttggg cgctaggtga atcatagagt tccttcaaaa
    30181 gactctggag attttcagat ttatttgctg gcttgctagt tgatcctttt gctacagatt
    30241 gcgtgttatt gtcactagct gttgaagaat agcttaactg actcggttgc atattttttc
    30301 cagccagatg tgctttagct gctgctaatt cactagcaga taaatcgctt ttgggaatgt
    30361 agtgatagtg acctccatga ggaacgatat aagcattacc cgtatcttcg ataatatcag
    30421 ctggattaaa gacataacca tcatttgtcg tatatcgtcc ctgagacctt gctacagcaa
    30481 cattagagtt aaccttctca ttatctttga catgttcttg tttttgacga ttgatttcat
    30541 ctttagttcg aacattatca gcatgagctg catctttcag gtagacataa tattttccat
    30601 cgaccttgat gatataacca cccttgactt cattgacaat atcagcgtct ttaagttgat
    30661 agtttggatc cttcatcaag agttcttcac taaagagggc atcataagga actttcccat
    30721 tatagtaatg atagtggtca ccgtgtgacg ttacatagcc ctgatctgta attttgatta
    30781 caatttgctc ggcctgaatt ccttctttct ggctaatctg gtctggtgtc aagttttcac
    30841 ttttctgact tgactggctg ccatctacat aagagacacg attattgtcc ttattttcct
    30901 gcgaacgatg ctggtttagt gcataggcac atagactcaa ggatacgata acagctgatc
    30961 cagctgctat atatttttta ctgaatttca taaatccctc atttcaataa atgatgaagt
    31021 tttttctcaa cttcttttac tttattaaat agttttctaa accggttatt taaccattta
    31081 attaaccagt aaatagttta ctatttaaaa ttacttttgt caagtttctg ttacgttttt
    31141 ctaaaaaaga gaagtaacac gaaaggttac ttctccataa aatatgctcc attcatttta
    31201 ctactgtata ggagtcggtt gactttcttt taacaaagcc aagagactat ctacttctgc
    31261 tgaaatagtg ttattatctt tcgttccgag aagaagacta ctttttagac cagtcaatgt
    31321 ctccacagca ttttgtctaa tactagaatc tgttactttt tctagcaagg cctccgcctc
    31381 tgctatctta gcgttaataa cagaatgctc tacttgagga atttcagcct catctgtagt
    31441 atcttcagct tcttcctctg tctcttccgt atcagtgctt ggtttataaa gattatctgc
    31501 tgaaggattt aaaccagcgt gattctcttt ttcatcagat tcagggtgag ttggctcact
    31561 tacttcatca tgttccttat cttcctcagg tttttctgtc tgaggtttct cctcgtttgg
    31621 ttt
//
LOCUS       DAVBGY010000019        28495 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVBGY010000019 DAVBGY010000000
VERSION     DAVBGY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28495
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.019"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            408..1361
                     /locus_tag="VQ620_000912"
     CDS             408..1361
                     /locus_tag="VQ620_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2763564.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLISLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            1412..2602
                     /locus_tag="VQ620_000913"
     CDS             1412..2602
                     /locus_tag="VQ620_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET2763565.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGTDAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            2751..3122
                     /gene="rnpA"
                     /locus_tag="VQ620_000914"
     CDS             2751..3122
                     /gene="rnpA"
                     /locus_tag="VQ620_000914"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET2763566.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            3097..3921
                     /locus_tag="VQ620_000915"
     CDS             3097..3921
                     /locus_tag="VQ620_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET2763567.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAKIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            3940..4926
                     /locus_tag="VQ620_000916"
     CDS             3940..4926
                     /locus_tag="VQ620_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HET2763568.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            4977..5656
                     /locus_tag="VQ620_000917"
                     /pseudo
     CDS             4977..5656
                     /locus_tag="VQ620_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
     gene            5787..7244
                     /locus_tag="VQ620_000918"
     CDS             5787..7244
                     /locus_tag="VQ620_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2763569.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            7267..7548
                     /locus_tag="VQ620_000919"
     CDS             7267..7548
                     /locus_tag="VQ620_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2763570.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            7625..8110
                     /locus_tag="VQ620_000920"
     CDS             7625..8110
                     /locus_tag="VQ620_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2763571.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            8127..8792
                     /locus_tag="VQ620_000921"
     CDS             8127..8792
                     /locus_tag="VQ620_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HET2763572.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            8796..9659
                     /locus_tag="VQ620_000922"
     CDS             8796..9659
                     /locus_tag="VQ620_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HET2763573.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SRGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            9661..10365
                     /locus_tag="VQ620_000923"
     CDS             9661..10365
                     /locus_tag="VQ620_000923"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HET2763574.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVAWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            10544..12217
                     /locus_tag="VQ620_000924"
     CDS             10544..12217
                     /locus_tag="VQ620_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2763575.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            12329..13420
                     /gene="ulaG"
                     /locus_tag="VQ620_000925"
     CDS             12329..13420
                     /gene="ulaG"
                     /locus_tag="VQ620_000925"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HET2763576.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            13534..15510
                     /gene="tkt"
                     /locus_tag="VQ620_000926"
     CDS             13534..15510
                     /gene="tkt"
                     /locus_tag="VQ620_000926"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2763577.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVTDYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            15627..15926
                     /gene="yajC"
                     /locus_tag="VQ620_000927"
     CDS             15627..15926
                     /gene="yajC"
                     /locus_tag="VQ620_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET2763578.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVEGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            15973..16401
                     /locus_tag="VQ620_000928"
     CDS             15973..16401
                     /locus_tag="VQ620_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HET2763579.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            16403..16813
                     /locus_tag="VQ620_000929"
     CDS             16403..16813
                     /locus_tag="VQ620_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763580.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            17113..19764
                     /gene="adhE"
                     /locus_tag="VQ620_000930"
                     /gene_synonym="adhC"
     CDS             17113..19764
                     /gene="adhE"
                     /locus_tag="VQ620_000930"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HET2763581.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITEPSMEATSALMNHEGIATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
     gene            complement(20749..21057)
                     /locus_tag="VQ620_000931"
     CDS             complement(20749..21057)
                     /locus_tag="VQ620_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2763582.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
     gene            complement(21054..21362)
                     /locus_tag="VQ620_000932"
     CDS             complement(21054..21362)
                     /locus_tag="VQ620_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2763583.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            complement(21367..22662)
                     /locus_tag="VQ620_000933"
     CDS             complement(21367..22662)
                     /locus_tag="VQ620_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2763584.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTKFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGIVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            complement(22680..24089)
                     /locus_tag="VQ620_000934"
     CDS             complement(22680..24089)
                     /locus_tag="VQ620_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET2763585.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTEDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSLCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            24247..24975
                     /locus_tag="VQ620_000935"
     CDS             24247..24975
                     /locus_tag="VQ620_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2763586.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            25435..25629
                     /locus_tag="VQ620_000936"
     CDS             25435..25629
                     /locus_tag="VQ620_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2763587.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSDVTLITGKNGTGKSTLL"
     gene            complement(25995..27305)
                     /locus_tag="VQ620_000937"
     CDS             complement(25995..27305)
                     /locus_tag="VQ620_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET2763588.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNFAITNGTLDVLAQHILYRTRTYPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTSIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            27542..28414
                     /locus_tag="VQ620_000938"
     CDS             27542..28414
                     /locus_tag="VQ620_000938"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET2763589.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT         8935 a         5071 c         5943 g         8546 t
ORIGIN      
        1 cgctgaacac caacaagacg cttaaattgt gcattggtta gttgtttact ttcttcataa
       61 attttattgt atcatatttt tgtttcacag gaagtctaat aaataaaaat tctttaaaat
      121 tttgttggga aaatgggaaa agatataaat ggaaacttag tataacactt ggaaaactct
      181 gtaaaaatag taagtcaatt catgtcccgt tttttctcta gggaagttgg gcggctatat
      241 ttatatgacc atttgtcgta ataaacatcc ctaccaaggg tcaatagaaa taaattcaac
      301 gaaacttttg ctgtcagatt tagatgccca atttgtcatt tatactcttt catataggac
      361 tttctataag gatggaaaaa agaagggaaa tttgataaga taggaatatg gattttgaaa
      421 aaattgaaca agcttatacc tatttactag agaatgtcca agtcatccaa agtgatttgg
      481 cgaccaactt ttatgacgcc ttggtggagc aaaacagcat ctatctggat ggtgaaactg
      541 agctaaacca ggtcaaagac aacaatcagg cccttaagcg tttagcacta cgcaaagaag
      601 aatggctcaa gacctaccag tttctcttga tgaaggctgg gcaaacagaa cccttgcagg
      661 ccaatcacca gtttacaccg gatgctattg ctttgctttt ggtgtttatt gtggaagagt
      721 tgtttaaaga ggaggaaatt actatcctcg aaatgggttc tgggatggga attctaggcg
      781 ctactttctt gatctcgctt gataaaaagg tggattactt gggaatggaa gtggatgatt
      841 tgctgattga tctggcagct agcatggcag atgtaattgg tttgcaggct ggctttgtcc
      901 aaggagatgc cgttcgtcca caaatgctca aagaaagcga tgtggtcatc agtgacttgc
      961 ctgttggcta ttatcctgat gatgccgttg cgtcgcgcca tcaagttgct tctagccaag
     1021 aacatactta cgcccatcac ttgctcatgg aacaagggct taagtacctc aagtcagacg
     1081 gatacgctat ttttctagct ccgagtgatt tgttgaccag tcctcaaagt gatttgttaa
     1141 aagaatggct gaaagaagag gcgagtctgg ttgctatgat tagtctgcct gaaaatctct
     1201 ttgctaatgc taaacaatct aagactattt ttatcctaca gaagaaaagt gaaatagcag
     1261 tggagccttt tgtttatcca cttgctagct tgcaagatgc aagtgtttta atgaaattta
     1321 aagaaaattt tcaaaaatgg actcaaggta ctgaaatata aaatagattt tgttataata
     1381 gttgaaaacg cttaaaaagg ggtatcatgt tatgacaaaa acaattgcaa tcaatgcagg
     1441 aagttcaagt ttgaaatggc aattatactt aatgccagag gaaaaagtgt tagccaaagg
     1501 tttgattgaa cgtatcggtt tgaaagattc aatttcaact gtaaaatttg acggccgttc
     1561 tgaacaacaa attttggata tcgaaaatca tacacaagct gttaaaattt tattggatga
     1621 cttgattcgt ttcgatatta tcaaggctta tgacgagatt acaggtgttg gccaccgtgt
     1681 tgtagcaggt ggagaatatt tcaaagaatc aacagttgtt gagggagatg ttttagaaaa
     1741 agttgaagag ttgagtttgt tggctcctct acacaacccg gccaatgcag caggtgttcg
     1801 tgctttcaag gaattgttgc cagacattac cagtgtagtt gtttttgata cttccttcca
     1861 cacaagtatg ccagagaaag cttatcgcta ccctctacca acaaaatatt acacagaaaa
     1921 caaggttcgt aaatacggtg ctcatggtac aagtcaccag tttgtagcag gagaagctgc
     1981 aaaactcttg ggacgtccat tagaagactt gaagttaatt acctgtcata ttggtaacgg
     2041 aggctcaatt acagctgtga aagccggcaa atctgtagac acttctatgg ggttcactcc
     2101 tcttggtggt attatgatgg gaacgcgtac aggggatatt gatccagcta tcattcctta
     2161 tttaatgcaa tatacagagg attttaacac accagaagat atcagtcgtg ttcttaaccg
     2221 tgaatcaggt cttttgggag tttctgctaa ttctagcgat atgcgcgata tagaagcagc
     2281 tgtagcagaa gggaatcacg aggctagctt ggcttatgaa atgtatgttg accgtatcca
     2341 aaaacatatc ggtcagtacc ttgcagtgct aaatggaaca gatgccattg ttttcacagc
     2401 aggtgtcggt gaaaatgcag agaatttccg tcgtgatgta atctcaggga tttcgtggtt
     2461 tggttgtgat gttgatgatg aaaagaatgt ctttggcgtt acaggagaca tctcaacaga
     2521 ggcagctaaa atccgtgtct tggttattcc aacagatgaa gaattagtca ttgcccgtga
     2581 cgttgaacgc ttgaaaaaat aagtgaaact aaaaaaatat tcaatacaag gagttgggaa
     2641 agttattttt ccagcttctt tttctgatga aattgtccaa aaccttgcta tgattggctt
     2701 ttttgaaaaa tatggtataa tagtagtaat ttaatagatg gagttgagtt ttgaagaaaa
     2761 actttcgtgt aaaaagagag aaagatttta aggcgatttt caaggagggg acaagttttg
     2821 ctaatcgcaa atttgtggtc taccaattag aaaaccagaa aaaccatttt cgagtaggtc
     2881 tatcagttag caaaaaactg gggaatgccg tcactagaaa tcaaattaag cgacggattc
     2941 ggcatattat ccagaatgca aaagggagtc tggtagaaga tgtcgacttt gttgtcattg
     3001 ctcgaaaagg agtcgaaacc ttgggatacg cagagatgga gaaaaatcta ctccatgtat
     3061 taaaattatc aaagatttac cgggaaggaa atgggagtga aaaagaaact aaagttgact
     3121 agtttgctag gactgtctct gttaatcatg acagcctgtg cgactaatgg ggtaactagc
     3181 gatattacag ccgaatcggc tgatttttgg agtaaattgg tttacttctt tgcgaaaatc
     3241 attcgctttt tatcgtttga tattagtatc ggagtgggga ttattctctt tacggtcttg
     3301 attcgtacag tcctcttgcc agtctttcag gtgcaaatgg tggcttctag gaaaatgcag
     3361 gaagctcagc cacgcattaa ggcgcttcga gaacaatatc caggtcgaga tatggaaagc
     3421 agaaccaaac tagagcagga aatgcgtaaa gtatttaaag aaatgggtgt cagacagtca
     3481 gattctcttt ggccgatttt gattcagatg ccggttattt tggccttgtt ccaagcccta
     3541 tcaagagttg actttttaaa gacaggtcat ttcttatgga ttaaccttgg tagtgtggat
     3601 acaacccttg ttcttccgat tttagcagca gtattcacct ttttaagtac ttggttgtcc
     3661 aacaaagctt tgtctgagcg aaatggcgct acgactgcga tgatgtatgg gattccagtc
     3721 ttgattttta tctttgcagt ttatgcgcca ggtggagtcg ccctatactg gacagtgtct
     3781 aatgcttatc aagtcttgca aacctatttc ttgaataatc cattcaagat tatcgcagag
     3841 cgcgaggccg tagtacaggc acaaaaagat ttggaaaata gaaaaagaaa agccaagaaa
     3901 aaggctcaga aaacgaaata aataaggagg aatctggtaa tggtagtatt tacaggttca
     3961 actgttgaag aagcaatcca gaaaggattg aaagaattag atattccaag aatgaaggct
     4021 catatcaaag tcatttctag ggagaaaaaa ggctttcttg gtctatttgg taaaaaacca
     4081 gcccaagtgg atattgaagc gattagtgaa acgactgttg tcaaagcaaa tcaacaggta
     4141 gtaaaaggcg ttccgaaaaa aatcaatgat ttgaacgagc ctgtgaagac ggttagtgaa
     4201 gaaaccgttg accttggtca tgtggttgat gctattaaaa aaatagagga agaaggtcaa
     4261 ggtatttctg atgaagtcaa ggctgaaatc ttaaaacatg aaagacatgc cagcactatc
     4321 ttagaagaaa ctggtcacat tgagatttta aatgaacttc aaatcgagga agcgatgagg
     4381 gaagaagcag gcgctgatga ccttgaaact gagcaagatc aaactgaaaa tcaagacttg
     4441 aaagagatgg gcttgaaggt cgagcaaagt tatgatattg cccaggtggc tacggatgtg
     4501 actgcctatg ttcaagcgat tgtggatgac atggatgttg aagctacact ttcaaatgat
     4561 tataaccgtc gtagcatcaa tctacaaatt gacaccaacg aaccaggtcg tattatcggc
     4621 taccatggta aagtcttgaa ggccttgcaa ctgttggctc aaaattatct ttacaaccgc
     4681 tattccaaaa ccttctacgt tacaatcaat gtcaatgatt atgtcgaaca ccgtgcagaa
     4741 gtcttgcaga cctatgcgca aaaattggcg attcgtgttt tagaagaagg acgtagtcat
     4801 caaacagatc caatgtcgaa tagtgaacgc aagattatcc accgtattat ttcacgtatg
     4861 gatggcgtga ccagttactc tgaaggtgat gagccaaatc gctatgtcgt tgtagataca
     4921 gaataagtaa aatcagggtt gacctgattt tttgctagct agaggaggtt aaactaatgt
     4981 tgaataagat aagagactat ttagactttg ctggtttgca gtaccgtaat cctgataaag
     5041 cgggagcaga gcgagagaag atgctggcat tccgccacaa aggacaagag gcccgaaagg
     5101 cttttacaga actggccaaa gcctttcaag caagccatcc aggatggcaa ctccaacaga
     5161 ctagtcagtg gatgaatcag gcccagcgtt tgagaccaca tttttgggtt tatctacaga
     5221 gagacgaaca agtgacagaa cctatgatgg ccttacgttt gtatgggaca tctactgact
     5281 ttggaatttc tttggaagtc agtttcatcg aacgtaagaa ggatgagcaa acactgggca
     5341 agcaggccaa agttttagac attccaaccg ttaaagggat ttattatcta acctactcta
     5401 atggtcaaag tcaacggtgg gaggcgaatg aagaaaagcg tcgtacttta cgcgagatgg
     5461 tcaaagtcaa cggtgggagg cgaatgaaga aaagcgtcgt actttacgcg agaaggtgag
     5521 aagtcaagaa gttcgaaaag ttttagtgaa ggtagatgtt cctatgacag aaaattcgtc
     5581 tgaagaagaa atcgtagaag gcttattgaa gtcttattct aaaattcttc cctattatct
     5641 agctacgaga aaataagata atttgtaaaa catcataaat catacagtcc aagagtgaac
     5701 agtccgctgt gtaattcttg gtctttttgt ttgcgctttc gcattatata ataaacttac
     5761 aaaaacaatt caaaaggaga acaattatgg aagtcgtttc aagtgttcta aattggtttt
     5821 ctagcaatat tttgcagaat cccgcatttt tcgtaggttt attggtgttg ataggatatg
     5881 cacttttgaa aaaacctgcc catgacgttt tttcagggtt tgttaaagca acagtagggt
     5941 atatgttgct taacgtgggt gctggtggtt tggttacaac ctttcgtcca atcttagcag
     6001 ctcttaacta caaattccaa attggtgcag cggttatcga cccttacttt ggacttgctg
     6061 cagcaaacaa caaaattgca gcagagtttc cagattttgt tggaactgca actacagctc
     6121 tattgattgg ttttggaata aatatcttgc tcgtagctct tcgaaagatt acgaaggtaa
     6181 gaaccctctt tattactggt cacatcatgg tacaacaagc tgcaacagta tctcttatgg
     6241 ttctattctt agtaccacaa ttgcgcaatg cttacggtac agcagcgatt ggtatcatct
     6301 gtggacttta ctgggcagtt agttcaaata tgactgttga ggcaactcaa cgcttgactg
     6361 gtggtggcgg atttgcgatt ggtcaccaac agcaatttgc aatctggttt gtagataaag
     6421 tagcaggacg ctttggtaag aaagaagaaa gtttagacaa tcttaaatta cctaagttcc
     6481 tctcaatctt ccacgataca gttgttgcat ctgctacctt gatgctcgta ttcttcggag
     6541 ccattctttt aatcttgggt ccagacatta tgtctaataa agaagtcatc acttcaggaa
     6601 ctctattcaa tcctgctaaa caagatttct ttatgtacat tatccaaaca gcctttacct
     6661 tctcagttta cttgttcgtt ttgatgcaag gtgtccgaat gttcgtatct gagttgacaa
     6721 acgccttcca aggtatttca aacaaattgt tgccaggttc attcccagcg gttgacgttg
     6781 cagcttctta tggatttggt tctccaaatg ctgtcttgtc aggatttacc tttggtttga
     6841 ttggtcaatt gattacaatt gttttgctca tcgtctttaa aaatccgatt cttattatta
     6901 caggatttgt accagtgttc tttgacaatg cagccattgc ggtctacgct gataaacgcg
     6961 gcggatggaa agcggctgtt atcctttcct ttatatcagg tgtccttcaa gttgctctag
     7021 gagctctttg tgtggccctt ctcgatttgg catcttatgg tggctaccat ggaaatatcg
     7081 actttgaatt cccatggctt ggatttggat atatcttcaa ataccttggt attgttggtt
     7141 atgtacttgt gtgtctcttc ttgcttgtta ttcctcaact tcaatttgcc aaagcaaaag
     7201 ataaagagaa atattacaac ggtgaagttc aagaagaagc ttagtatcta gaaaaggaga
     7261 aataaaatgg ttaaagtatt agcagcgtgc ggaaatggaa tgggttcatc aatggttatc
     7321 aagatgaagg ttgaaaatgc tctccgtaag cttaatcaaa cagattttac agtcaattca
     7381 tgcagtgtcg gtgaagctaa aggtttagca gtaggatatg acattgtaat cgcttctctt
     7441 catttgattc aagaattgga agggcgaact aatgggaagt taattgggct tgataacttg
     7501 atggatgata aagaaatcac cgaaaaactc agtcaagcac tacagtaaaa ggttggaggg
     7561 ggctggacag aaactgagag ttatcgtttc tgtccagccc cctctttaaa taaaggaggc
     7621 agatatgaat ttaaaacaag ctttaattga caatgactcg atccgactag gtttagaggc
     7681 taacaattgg aaagaagcag tcaaggtagc agtagatccc ttgattgaaa gtggggcaat
     7741 tttgccagag tattacgatg ctatcattga atcgactgaa gagtatgggc cttactatat
     7801 cttgatgcca ggtatggcta tgccccacgc tagacctgaa gctggtgtgc aaagtgatgc
     7861 cttttcattg attaccttac aaaatcctgt tgtattttca gatgggaaag aggtatctgt
     7921 tttgttggca ctagcagcaa caagttcaaa aattcacaca agtgtagcca ttccacaaat
     7981 tattgccctg tttgaattag aagattctat tgcacgttta caggcttgcc agactaaaga
     8041 agatgtcttg gctatgattg aagaatctaa ggatagccct tatctcgaag gattggattt
     8101 ggaaagttag aaagaggaat aaagaaatga caaaaagaat acctaattta caagttgcat
     8161 tagaccattc agacttgcaa ggagcgatta aagcagctgt ttctgttggt caggaagtag
     8221 atattatcga agctggaact gtttgcttgc ttcaagttgg aagtgaactg gctgaagtct
     8281 tgcgtagcct tttcccagat aagattattg tggcagacac aaaatgtgct gatgctggtg
     8341 gaacagttgc taaaaataat gcggttcgtg gagcagactg gatgacttgt atctgttgtg
     8401 caaccatccc tactatggaa gcagctctaa aggctatcaa gactgaacga ggagaacgag
     8461 gcgaaatcca gatcgagctt tatggcgatt ggacttttga acaagctcag ctttggctag
     8521 atgcaggtat ctcacaagct atttatcacc aatctcgtga tgctcttctt gctggtgaaa
     8581 cttggggtga aaaagacctt aataaggtta aaaaactcat tgacatgggc ttccgtgtat
     8641 ctgtaacagg tggtctagat gtagatactc tcaaactctt tgaaggtgtt gatgtcttta
     8701 cttttatcgc aggtcgtgga attacagagg ctgcggatcc agcaggagca gcgcgtgcct
     8761 tcaaggatga aatcaaacga atttgggggt aaatcatggt acgtccaatt ggaatttatg
     8821 aaaaggcaac cccaacacac tttacttggc tagaacgttt aaattttgcc aaggagttag
     8881 gctttgattt tgtcgagatg tctattgacg aacgtgacga gcgtttagca agacttgact
     8941 ggagtaagga agaacgcttg gaagttgtca aagaaatcta tgaaactggt gttcgtattc
     9001 cttctatctg tttttcaggc catcgtcgct acccattggg ttcaaaagat ccagttctag
     9061 aggaaaaatc tctagaactc atgaaaaaat gtatcgaatt agctcaagac ttgggagttc
     9121 gtacgattca attagctggt tacgatgttt actatgagga aaagtcaccc cagacacgcc
     9181 aacgttttat caaaaatttg agaaaagcct gtgactgggc tgaagaagct caggtggtac
     9241 ttgctattga aattatggat gatcctttca tcaatagcat cgaaaaatat ttggctatag
     9301 aaaaagagat tgactctccc ttcctctttg tatatccaga tattggtaat gtgtctgcat
     9361 ggcataatga tatctatagt gagttttatc ttggtcatca tgccatcgca gctctccatc
     9421 tcaaggatac ttatgcagtg acagaaagtt caaggggcca gttccgagat gtaccttttg
     9481 ggcaaggttg tgtcaaatgg gaagaagctt tcgatatttt aaaggaaacc aattataatg
     9541 gacctttcct aatcgaaatg tggtctgaaa attgtgaaac agtagaagaa acacgcgcag
     9601 ccattcaaga ggcgcaagct tttctctatc cactcattaa gaaagcaggt ttgatgtaag
     9661 atgaatcaag taatcaatgc tatgcgtaaa cgagtctgtg atgccaatca atcattgcca
     9721 aaacatggac ttgtcaaatt tacctggggg aatgtatctg aagtcaatcg cgaactcggt
     9781 gtcattgtta tcaaaccatc aggcgtggat tatgacgaat tgacacctga aaacatggta
     9841 gtgactgatc tagatggtaa gatcctagaa ggggatttaa gaccatcttc cgacctccca
     9901 actcatgtgc aattatataa ggcttggtca gaaattggta gtgtggttca cacccattcg
     9961 acagaagctg ttgcttgggc tcaggcaggt cgtgatattc ctttctacgg aacaacccat
    10021 gcagattatt tctacggttc aatcccttgc gcccgtagtt tgaccaagga cgaagtagaa
    10081 gtggcctatg aaaaagatac tggcctggtt atcgtagaag agtttgaaca tcgcggactt
    10141 aacccggttg aagtaccagg aattgttgta cgcaatcacg gtccattcac ctggggcaaa
    10201 aatccagaga atgctgttta tcactctgtc gtactagagg aagtatcaaa gatgaatcgc
    10261 tttacagaac aaatcaatcc aagagttgga cctgctcccc agtacatact agaaaaacac
    10321 taccaacgta aacatggacc aaatgcttat tatggtcaaa agtaagaacg atgaaggagg
    10381 agaaaaagat aaatttagct cctcttttta catttgattt ttattgagag taaagttgga
    10441 gttgaagtaa ttttaaaaga ttttttagaa atagcgcttg atatatatat ggtaaaataa
    10501 aaagaattgc tgtgatatca atagatttgg gggatttttt aatatggtac tggataaggc
    10561 aagttgtgat ttgcttcaat atttgatgga tcaagaaacg tccaaaacga ttatggcgat
    10621 ttcgaaagat ttgaaagagt caagaaggaa aatttattat cacattgaca aaatcaatgc
    10681 tgctctgggt gacgaggcgc ttcacatcat tagtattcca cgaattggta ttcacttaac
    10741 ggaagagcag agagatgctt gttgtaaact attatcggaa gtagattcgt acgattatat
    10801 catgagtgcg catgaacgta tgatgataat gttactatgg ataggtattt ctaaagaacg
    10861 tattacgatt gaaaaattga tagagttaac agaggtatct aggaatactg ttctcaatga
    10921 tttgaatagt attcgttatc aactaacttt ggaacaatat caggtgatct tgcaagtgag
    10981 caagtcacag ggataccacc ttcatgccca ccctcttaat aaaattcagt atcttcaatc
    11041 gcttctatat catattttta tggaagaaaa tgccactttt gtatctattt tagaagataa
    11101 gatgaaagag aggttagatg atgagtgttt gctttctgtt gaaatgaacc aattttttaa
    11161 ggaacaggtt cctttagttg aacaagattt agggaagaaa ataaaccatc atgaaataac
    11221 ttttatgttg caggttctac cttatttgct gttaagctgt cataatgttg aacagtatca
    11281 agaaagacat caggatatag agaaagaatt ttctttgata agaaaaagaa tagagtatca
    11341 ggtgtctaag aaattaggag aacggttgtt tcaaaagttt gaaatttctt tgtcaggact
    11401 tgaagtttct cttgtagctg ttctcctcct ctcctatcgt aaagatttgg atattcatgc
    11461 agaaagtgat gattttcggc aattaaaact tgctttagaa gaatttatct ggtattttga
    11521 atcacaaatc cgaatggaga ttgagaacaa ggatgatttg ttacgaaatt tgatgatcca
    11581 ctgtaaagcc ttgttattta gaaagactta cggtattttt tctaaaaatc ctctaacaaa
    11641 acaaattcga tccaagtatg gagaattatt tttagtcact agaaaatctg cggaaatttt
    11701 agaaggagca tggtttattc ggctaacaga cgatgatatt gcctatttga cgattcatat
    11761 tggaggattt ttaaaatata caccatcatc tcaaaaaaat atgaaaaaag tttatctcgt
    11821 ttgtgatgaa ggtgttgcgg tttcgagact tttgctgaaa caatgcaaac tttattttcc
    11881 aaatgagcaa attgacactg tatttacaac agaacaattt aagagtgtgg aagatattgc
    11941 acaagttgat gtagtgatta ctactaatga tgatttggat agcagatttc cgattttaag
    12001 ggttaatcct atccttgaag cagaagatat tttgaaaatg ctagactatc ttaaacacaa
    12061 tatatttcgt aataagagca aaagtttcag tgaaaatctt tctagtctta tttcgtctta
    12121 tattgtagac agcaagttgg ctagtaagtt ccaagaagag gttcaaacac ttataaatca
    12181 agaaatagta gttcaagctt ttttggaaga tatttgaagg acagtccaat gatgaacaca
    12241 aacctgtgtt tttcttggtc ttttttagtg ttttgaaggg tggtatacta atctcaaaga
    12301 taacaattat atccaaagga ggcaacatat gccaaacgtc aaagaaatta caagagagtc
    12361 atggatttta gccactttcc cagagtgggg aacatggttg aacgaagaaa tcgaagaaga
    12421 agtcgtacct gaaggcaact ttgccatgtg gtggctaggc aactgtggta cttggattaa
    12481 gacaccagct ggtgctaacg ttgtcatgga cctttggtca aaccgtggaa aatcaaccaa
    12541 aaaagtgaaa gatatggttc gtgggcacca aatggcaaat atggcaggtg ttcgtaagct
    12601 gcaaccaaac ttgcgtgttc agccaatggt tatcgatcca tttgctatca acgaactaga
    12661 ctattactta gtttcacact tccacagtga tcatatcgac ccatacacag ctgcagcaat
    12721 tctcaataat cctaagttag agcatgttaa gtttatcggt ccttaccact gtggacgaat
    12781 ctgggaagga tggggtgttc caaaagaacg tatcatcgtt gttaaaccag gtgacactat
    12841 cgaattaaaa gatatgaaga ttcatgcagt agaatcattt gaccgtactt gcttggtaac
    12901 tctcccagtg aacggtgctg atgagacagg cggtgaactt gctggcttgg ctgttacaga
    12961 tgaagaaatg gctcaaaagg ctgttaacta tatctttgaa acaccaggtg gaaccatcta
    13021 tcatggtgca gattctcact tctcaaacta ttttgcaaaa catggtaaag actttaaaat
    13081 tgatgttgct ttgaataact atggtgaaaa tccggtaggt atccaagaca aaatgacatc
    13141 tatcgacctt cttcgtatgg cagaaaatct gcgtaccaaa gtcattatcc cagttcacta
    13201 tgatatctgg tctaacttca tggcttctac taatgagatt ctagaacttt ggaaaatgcg
    13261 aaaagatcgc ttgcaatacg atttccatcc atttatctgg gaagttggcg gtaagtacac
    13321 ttatcctcaa gatcaacact tagtagaata ccatcatcca cgtggttttg atgattgttt
    13381 tgaacaagac tctaacattc aatttaaagc tttgctataa tataaaaata ttctttggag
    13441 gttatccgta tgttgcatga tacggataat tttcatataa tagtaaaata gaatgtgtga
    13501 ttcaataatc acctcaaata gaaaggaaat tctatgtcaa atctatctgt taatgcaatt
    13561 cgttttctag gtattgacgc cattaataaa gccaactcag gtcatccagg tgtggttatg
    13621 ggagcggctc cgatggctta cagcctcttt acaaaacaac ttcatatcaa tccagctcaa
    13681 ccaaactgga ttaaccgcga ccgctttatt ctttcagcag gtcatggttc aatgctcctt
    13741 tatgctcttc ttcacctttc tggttttgaa gatgtcagca tggatgagat taagagtttc
    13801 cgtcaatggg gttcaaaaac accaggtcac ccagaatttg gtcatacggc agggattgat
    13861 gctacgacag gtcctctagg gcaagggatt tcaactgcta ctggttttgc ccaagcagaa
    13921 cgtttcttgg cagccaaata taaccgtgaa ggttacaata tctttgacca ctatacttac
    13981 gttatctgtg gagacggaga cttgatggaa ggtgtctcaa gcgaggcagc ttcatacgca
    14041 ggcttgcaaa aacttgataa gttggttgtt ctttatgatt caaatgatat caacttggat
    14101 ggtgagacaa aggattcctt tacagaaagt gttcgtgacc gttacaatgc ctacggttgg
    14161 catactgcct tggttgaaaa tggaacagac ttggaagcca tccatgctgc tatcgaaaca
    14221 gcaaaagctt caggcaagcc atctttgatt gaagtgaaga cggttattgg atacggttct
    14281 ccaaacaaac aaggaactaa tgctgtacac ggcgcccctc ttggagcaga tgaaactgca
    14341 tcaactcgtc aagccctcgg ttgggactac gaaccatttg aaattccaga acaagtatat
    14401 gctgatttca aagaacatgt tgcagaccgt ggcgcatcag cttatcaagc ttggactaaa
    14461 ttagttacag attataaaga agctcatcca gaactggctg cagaagtaga agccatcatc
    14521 gacggacgtg atccagtcga agtgactcca gcagacttcc cagctttaga aaatggtttt
    14581 tctcaagcaa ctcgtaattc aagtcaagat gccttgaatg ttgtggcagc taaattacca
    14641 acctttctag gtggatcagc tgaccttgct cactcaaaca tgacttatat caagacggat
    14701 ggacttcaag acgatgccaa tcgcttgaat cgtaacattc agtttggtgt tcgtgaattt
    14761 gcaatgggaa caatcttgaa cgggatggcc cttcatggtg gacttcgtgt atacggtgga
    14821 actttcttcg tcttctctga ctatgtgaag gcagctgtcc gcttgtcagc cttacaagga
    14881 cttcctgtga cttatgtctt tacccatgat tcaatcgcag ttggggaaga tggtccgact
    14941 catgaaccag ttgagcattt agcaggtctt cgtgctatgc caaatctaaa tgttttccgt
    15001 ccagcagatg cgcgtgaaac gcaagcagct tggtaccttg cagtgacaag tgagaaaaca
    15061 ccaactgccc ttgtcttgac acgtcaaaat ttgactgttg aagatggaac agacttcgac
    15121 aaggttgcta aaggtgctta tgttgtatat gaaaatgcag ccgactttga taccatcttg
    15181 attgcgacag gttcagaggt taatcttgct gtctcagctg ctaaagaatt ggctagtcaa
    15241 ggcgaaaaaa tccgcgtagt cagcatgcca tctacagatg tctttgataa acaagatgca
    15301 gcttacaagg aagaaattct tccaaatgca gtccgccgtc gtgttgcagt cgaaatgggt
    15361 gcaagtcaaa actggtacaa atatgttggt ctcgatggtg ccgttctagg tattgatacc
    15421 ttcggagcct ctgccccagc accaaaagta ttggcagaat atggctttac tgtagaaaat
    15481 cttgtaaaag ttgttcgaaa cttgaaataa tcctaaaaat cagggcgtaa gctctggttt
    15541 ttcttaccag aaaagtaagg tacaatcttg taaaagtagc tgaaatttga tatagtagtc
    15601 ctatgtaaaa gacaaaggag aaaacaatga atccaaatat tactttttta atcatgcttg
    15661 taggtatgat ggccttgatg ttctttatgc aacgttctca aaagaaacaa gctcaaaagc
    15721 gtatggaaag cttaaataaa ctacaaaaag gctatgaagt gattacaatc ggtggacttt
    15781 atggaacagt cgatgaagta gatacggaga agggaacaat agttcttgac gtagagggag
    15841 tttacttgac ttttgaacta gctgctatca agacagtatt accgctgaaa gaaacagctt
    15901 cactagaagg cgcaattgaa aaataagacg ggatcacgaa ctcccgtttt tctataaaag
    15961 aaaggaaatg ggatgaaaaa attagtcttt gtctgtctgg gaaatatttg ccgtagccct
    16021 atggccgagt ttgttatgaa atcaatgaca gataactacg aaatccaaag tcgagcaact
    16081 tcctcttggg aacatggcaa tccgattcat aaggggactc agggaatttt tcaagagtat
    16141 gagattcctt atgacaagaa caagacatcg cttcagatta gtaaggaaga ttttgaagcc
    16201 tttgattata ttatcggaat ggacgcttca aatatttctg acttacgtca gatgtgtcca
    16261 gtagactgtc aagataagat ttactcattt tcatctgaaa gtgttccaga cccttggtat
    16321 acaggagatt ttgaagaaac ctatcgacgt gttcaagagg gctgtcaagc ttggttagaa
    16381 cgtttagaaa aggagagtta aggtggaaaa tcttacgaat ttttacgaaa agtatcgtgt
    16441 ctatctgact cgtccacgtt tagagctttt ggcagtagtt accattgttt tctgtgctgt
    16501 actcgtcttt tttctaaata ttccaggaaa aggtgtctta aaactcgata atggaacgat
    16561 tgtttatgat ggcagtcttg tccgcggtaa aatgaatggc caaggtacca ttaccttcca
    16621 aaatggagac caatatacag gtggcttcaa caatggagcc ttcaacggaa aaggtacctt
    16681 tcaatctaaa gaaggctgga cctacgaagg tgattttgta aatggtcagg ctgaaggaaa
    16741 agggaaacta acaacagaac aagaagtcgt ttatgaagga acttttaaac aaggcgtttt
    16801 tcaacaaaaa taaagcctcc ttatcaaagg aggtattatt agaatcgcaa ggtaagcgct
    16861 tacctgcaaa tccctttctt tccaaatccc tcttccaagc aagtttgtga aataaaaaat
    16921 atttgaaata aatttcacaa acttcaaaga taaaacctga taagaaaaga aaatgagaaa
    16981 agtttcacaa gagtttaaaa attctttgtg aaatgtttat gaaactgttt acaaagttga
    17041 aataatgcgt tataataaac ttgtgaaaaa attaacaaag gatatcgttc cttgaaagct
    17101 atggaggaaa atatggctga taaaaaaact gtgacaccag aggaaaagaa actcgttgct
    17161 gaaaaacacg tagatgagtt ggttcaaaaa gctctagttg cccttgaaga aatgcgtaaa
    17221 ttggatcaag aacaagttga ctacatcgtt gccaaagcat cagtagcagc tttggatgcc
    17281 cacggagaat tggctttaca tgcctttgaa gaaacaggac gtggtgtatt tgaagataaa
    17341 gcaactaaga acttgtttgc ttgtgaacac gtggtaaaca acatgcgtca cactaaaaca
    17401 gttggcgtta tcgaagaaga tgatgtaaca ggattgaccc tgattgctga accagttggt
    17461 gttgtttgtg gtatcactcc aacaacaaac ccaacatcaa cagcgatctt caaatcattg
    17521 atttcattga agacacgtaa tccaatcgtc tttgccttcc atccatcagc tcaagaatca
    17581 tcagctcatg cagctcgtat cgtccgcgat gcagctatcg cagctggtgc tcctgaaaac
    17641 tgtgtacagt ggatcactga accatctatg gaagcaacta gtgcccttat gaaccacgaa
    17701 ggtattgcaa caatccttgc aacaggtggt aatgccatgg ttaaagccgc atactcatgt
    17761 ggtaaaccag ctcttggggt aggtgccgga aacgttccag cttatgttga aaaatcagca
    17821 aacattcgtc aagcagcaca cgatatcgtc atgtctaaat catttgataa cggtatggtc
    17881 tgtgcatctg aacaagcagt tatcattgat aaagaaattt acgatgaatt tgtagcagag
    17941 ttcaaatctt accacactta ctttgtaaac aaaaaagaaa aagctcttct tgaagagttc
    18001 tgcttcggcg tcaaagcaaa cagcaaaaac tgtgctggtg caaaattgaa cgctgacatc
    18061 gttggtaaac cagcaacttg gattgcagaa caagcaggat ttacagttcc agaaggaaca
    18121 aacattcttg ctgcagaatg taaagaagtt ggcgaaaatg agccattgac tcgtgaaaaa
    18181 ttgtcaccag ttattgcagt tttgaaatct gaaagccgtg aagatggtat tactaaggct
    18241 cgtcaaatgg ttgaatttaa cggtcttgga cactcagcag ctatccacac agctgacgaa
    18301 gaattgacta aagaatttgg taaagctgtt aaagctattc gtgttatctg taactcacct
    18361 tctacttttg gtggtatcgg ggacgtttac aatgccttct tgccatcatt gacacttgga
    18421 tgtggttctt acggacgcaa ctcagttggg gataacgtta gtgccattaa cctcttgaat
    18481 atcaaaaaag tcggaagacg gagaaataac atgcaatgga tgaaacttcc ttcaaaaaca
    18541 tactttgaac gtgattcaat tcaatacctt caaaaatgtc gtgacgttga acgtgtcatg
    18601 atcgttactg accatgccat ggtagagctt ggtttccttg atcgtatcat cgaacaactg
    18661 gaccttcgtc gcaataaggt tgtttaccaa atctttgcgg atgtagaacc ggatccagat
    18721 atcacaactg taaaccgtgg tactgagatt atgcgtgcct tcaaaccaga taccatcatc
    18781 gcactcggtg gtgggtctcc aatggatgct gccaaagtaa tgtggctctt ctacgagcaa
    18841 ccagaagtgg acttccgtga ccttgtccaa aaattcatgg atatccgtaa acgtgccttc
    18901 aagttcccat tgcttggtaa gaagactaaa ttcatcgcga ttccaactac atctggtaca
    18961 ggatctgaag taacaccatt tgccgttatc tctgataaag caaacaaccg taaataccca
    19021 atcgctgact actcattgac accaactgtg gcaatcgtag atcctgcttt ggtattgaca
    19081 gttccaggat ttgttgctgc tgatactggt atggacgtat tgactcacgc gacagaagca
    19141 tacgtatcac aaatggctag tgactacact gatggtttag cacttcaagc cattaaattg
    19201 gtctttgaaa atctcgaaag ctcagttaag aatgcagact tccactcacg tgagaaaatg
    19261 cataacgctt caacaatcgc tggtatggcc tttgccaatg ccttcctagg tatttctcac
    19321 tcaatggccc ataagattgg tgcgcaattc cacacaatcc acggtcgtac aaatgctatc
    19381 ttgcttccat acgttatccg ttacaacggt acacgtccag ctaagacagc aacatggcct
    19441 aagtacaact actaccgtgc agatgaaaaa taccaagata tcgcacgcat gcttggactt
    19501 ccagcttcta ctccagaaga aggggttgaa tcttacgcaa aagctgtcta cgaactcggt
    19561 gaacgtattg ggatccaaat gaattttaga gaccaaggaa ttgacgaaaa agaatggaaa
    19621 gaacattctc gtgaattagc cttcctggct tatgaagacc aatgttcacc agctaaccca
    19681 cgtcttccaa tggtagacca tatgcaagaa atcatcgaag atgcatacta tggctacaaa
    19741 gaaagaccag gacgccgtaa ataattgttt atcagtctag aagcaagaca aaaactcaat
    19801 ttgagggaaa gatccagtaa tttttctatg ataaaaggca tcctatcaag gtttttgaac
    19861 acctgatagg atgccttttt atgatattga ggcctttttg ccctttttga aaaactagaa
    19921 tagaaacaaa atatataata gattgaaact ggaatagtac acttctgctt ctaaaacatt
    19981 gttagaattc gatttgactg tcctgatcga tttgtcctat tcctatttca ttttactata
    20041 taaaaaatat agtatagtag actgaatcta aaatagtacg aaacaattgc taaaacattt
    20101 atagaaatta attttacttt cctgatagag ttgttcacat cttatttcaa ttcactatag
    20161 tttaatttaa gagtagtatt tactaaggcc caattaaaat caaagagcaa actagaaaac
    20221 gagtgccatt cagctcaaaa cactgatttg agattgcaga taagactagc ccctcattaa
    20281 caggtttacg ataaaacgat gacaaggtgt gttgcttttt gatttctaaa gagtataatg
    20341 atagatctct ataaaataag tgcgaaggaa atgagctttt atagtccttt cgttttaaaa
    20401 tactatctca gatgttctta tatcgacaag aagtttttga gtcattccct catcatacat
    20461 attaaataaa tagtggctca ttcaattttt tactagaata ataagctagt atagtaaact
    20521 gaaataagat ataaacaaat aaattggagc ttaacatcta tttccagcaa ttttttagaa
    20581 actgcagtgg actattctaa attcaacaca ttataaaaac tagagtaaaa gaaaaggatt
    20641 ggatcttgtg taatgcagga tctaatcctt tcaatcattt tgtccaactt ttggaggttc
    20701 ctacaatgta gccgtcatta ataaagacag atgggaacga cagtgttcct atttattttg
    20761 atagagatcg atgaattctt tagatagcaa ctgaataatc tctgttgaag ccatttggtc
    20821 ttctgcatgc ataaatagca aggagaatcc tattttttct ccagtagctt ctttttgtat
    20881 gagattagag tgaatcttgt gcgcttctac taaggagtct tccgcttctt caactttaat
    20941 tttcgcttct tttaaatttc ctgccttagc tagttggatg gcttcaataa aggatgattt
    21001 ggctgctcca ctattggcaa tgagctgaaa acagatatat tccatttctt ctgtcatctt
    21061 atttctccta tccatgcaag tgcttgttcc agaacttttg ctccattcat cattccgtaa
    21121 tcccgcatat caatggtatc tacagggata tttcctgcaa tttctttcac agcaagtaac
    21181 tcataacgaa tttgtggccc aattagaatg acatctgctt catggatatt ctttttcgct
    21241 tctgtcattg attttgcttg gatagaaatt tcaatcccac gttcagtcgc actttgttgc
    21301 atttttttaa caagcatact tgtcgacatt cccgcattac atactaataa aatttgtttc
    21361 ataatcttaa ccttccattt cttgctcaac aactttgtca ttaactttga taaatggaat
    21421 gtatagaaga actccaagtg caaagatgat gaattgaact agaactgctc tcacgtcccc
    21481 tgctgttgct aaccatgcat ttaagaatac tggtgtagtc caaggaactt gtataaatgc
    21541 aggactcatg aatcctgtaa ctgttgctaa gtagctgatt aaaataccaa ggactggaac
    21601 tgtgataaat ggaatagcta atgaaatgtt ataaacgatt gggtaaccga ataatactgg
    21661 ttcattgata ttgaagatac caggtccaaa agataattta gccacgtttt tagaaacagc
    21721 attgcgactc actaagaatg ttgctattaa taaacataat gtagatccac taccacccat
    21781 taaagcgaat gtttgtattt gtgataagtt gatgatgtgt ggaatggctt gtccattatt
    21841 tgctgcagtg atgttttcag taatgttaat taatagtaat ggttctagga tggcactgta
    21901 aataactgct tggtgaatac caaatagcca taacatattt cctaaagagt aaataataat
    21961 gaccccgatt aagcttgtac caatatgacg aattggttct tgaataaaga ttgtaatgat
    22021 tgagattaag ttcattccag ttatattgaa taataatgct gaaacaatcc caaataagga
    22081 gatgacagtc atgactggaa gtaatacgct aaatgatcta ctaacagctg gtggaatatt
    22141 ttcaccaagg ttcatttgta aagctttaac gtttgataat tcaatgaata attctgttgc
    22201 aataatcgct acgataaccc cggcgaacat tgcgcctgta cctgtgttgt tgaatgaaag
    22261 aacacctgaa atgtttacag catcttttgc tccgtcagga actacagaaa ctgtatttgg
    22321 catcatcaca attaaagaaa ctaatgatag cattgatgct gctaacgggt tttcgaaatc
    22381 tctgttttta gctaagaaat aaccaaccat tacagcaata atcatacctg aaatacttaa
    22441 agtaccgttt gcaattgtta ttccccaata ttggaatttt gttaatgtat ccccttggaa
    22501 aatccactta aataccgtgt tgttcaaaag aacgattaaa cctgccaaaa tatataatgg
    22561 cattactgtt acgaatgcat ctcttagggt ttttaaatga atttggttcc ctagtttacc
    22621 agcaaaggtg gcaaattgat ctataaattt tgagtttttc atctgaatat ctcctctctt
    22681 tactcctcaa tttctacttc aaaaccgtta ttgtcactta ctttgcgata ccatcttcct
    22741 gagctcttga tggttctctt ttgcgtttct aaatcaacgg agataaatcc gtaacgattc
    22801 ttatatgcat tattccaaga ccagcaatca aatgcggtcc aagcgtggta tccaaaacag
    22861 agacttccct cttcaatggc tttatgtagc catcttaaat gttcttcata aaattcaata
    22921 cgatacacgt catcgataac tccattttca tcgataaacc gtgcttcgtt ttcaacaccc
    22981 attccgttct cactgataaa ccatgggata ttaccatatt cttccttcac aatcatagca
    23041 atatcataaa tggctttcgg aaaaatttcc caaccacgat atggattcat gcgacgctcc
    23101 ggccattcat actctttgaa gtattggtct ggcatccagg gcgtctgata ttcctcagga
    23161 tttgcttgtg cttgaacacg ttttggatgg tagtagttta ctccaagaaa atcaaccgta
    23221 tttgatttca tcagttgaag ctctttttcg gtatgactcc ataacacgcc atctctctct
    23281 agctgtttta ccaatctttc tgggaaagtt cctttaacag ctggattcaa gaagactttg
    23341 ttaaagaagt cttctgtaaa tcgacttgct tctaagtctt ctggagaatt acttcttgga
    23401 taagcaggtg tcaagtttaa aataatccca atctttccat caagtcctaa tgagcgatat
    23461 agttgaatca cttttgcact agcaaggttt agattataga tgacttgtac ggcctctttt
    23521 ccctttcctt ttagatttgg ataatggaaa gcatataagt atcctgcttc tggaatgacc
    23581 attggctcat tgaaagttgt ccagtaatga accttatctc caaagcatgt gaaagcagtc
    23641 ttggcaaact tcacgaataa ctccactaca tgtttgcttt cccaaccacc gtatttttga
    23701 agaagttcca ctggtaaatc aaaatgatgt aaattcatca caagatccat ctggttcttt
    23761 ttagcttctt caatgatggc attgtagaaa gcaatacctt ttggatcagg ctcacctgtc
    23821 tctaaattct tgatgagtcg actccattgg atggaagtgc ggaaagaatt aactccaatt
    23881 tccttcatca agtggaaatc ttcggtgtaa gtatgaaaaa agttactggc tacaagaggt
    23941 ccgacattat cgaaaaaatc ttctggatgc gttttgaacc agtagtccat cacattttca
    24001 tgtacttttc catattgacc ttctgtctga ggtccgctag aagcagctcc ccaccaaaac
    24061 agattaggaa atactaactt ttttttcatg ctcaacctcc tttctattat gctatacata
    24121 aagtatagca taattttaaa atttgtctag acatttttaa aaattgttga tacatatatt
    24181 gtgtttgcct catttcttga actttgttat actactatta gaatattata gagagaacca
    24241 gattaaatga gtacaaaata tttatttatt tacaatgaga ttcgtgaaaa gattctctgt
    24301 aataaatata ccatgaacga acaattgcct gatgaaatga cattagctaa acagtttgcc
    24361 tgtagtcgaa tgacgatcaa aaaagcttta gacttgttag tttctgaggg cttaattttt
    24421 agaaaacgtg ggcagggaac ctttgttctc tctcgtggca gctcaaaaag aaaattaatc
    24481 gttccagaaa gagatatccg gggactgaca aaaatatctg aagatgctca ttctacaatt
    24541 gactcgagga ttattcactt caaattagaa tttgcaaatg aatttttagc agaaaaacta
    24601 caggtcgctt tgcagagtcc agtttataat atttaccgcc tgcgtattat tgacggtaaa
    24661 ccttatgttc tggaacaaac ttatatgagt accgatgtta ttccaggtat tactgaagat
    24721 attttacaaa aatcgattta caattacatt gaaggaaagt taggattgca tattgccagt
    24781 gctacaaaaa tcttgcgagc ttcttctagt tcagaaaatg agcaacatta cttgcagctc
    24841 cttccaacag aaccggtatt tgaagtagaa caagtggctt atttggataa cggaactccg
    24901 tttgagtact cgattagtcg tcatcgctat gatttatttg aatttaattc ttttgcatta
    24961 cgacattcct cctaggagaa aatgtgaaaa tgaagccaat cttttacagg ctctagttta
    25021 agaaaaattt aaaacagggc aagaaggtcc catctatgct taaatggttt ctcttttcta
    25081 aataagatgg ctttaaaaga gtgatcgttt tatccatcat gttgaaaaat atcttcgtat
    25141 agcttataga gtaggtactg aaattgttca cctgatctac ttcttatagt tatttagttt
    25201 taaatagtgt ttcaaacatt cttacactga cgagaagttt ttgagtcttt tcttgtaaca
    25261 catatagtat actgaggtta gaatagtaga ctgtgacttc taacaaattg ctagaaatga
    25321 atttcaatct cccaatttat ttgcttatat attattttaa tatattaaat aaattctgga
    25381 tcataatcat ttaaaaaaat tttatttttt tatttttcat tacgaataat atagatgaag
    25441 gggaaagagt atgaaaacag aactgtttct tttgctatta gttcaaaagg agaaaaaatg
    25501 aaagtagaaa atatttcgta tagggtggat catcgtatat tgtttgataa tatttctttt
    25561 gatacttcga gttcagacgt gacattaatt actggtaaaa atggtacagg aaagtcaact
    25621 ttactatagt ggattgaaac tagaatagta cacatctact tctaaaatat tgttagaaat
    25681 cgatttgact atcctgatct atttgttctg ttcttatttc atttcactat atctcaaatt
    25741 gagtatgacg aagtgcgctc ccatgtcctg ggaacgcact ttcttcatat ttttcatatt
    25801 cttgaatcca tcgataaaga ctattgggat gaatttttaa agttgaacta atcattttta
    25861 caggatgagg tttacagcag agagtttgaa ggctttatca aaggtttttc ttggcataat
    25921 gacttttcct cgtttccact taattttgtg tctactttat tataccaagt ccacccttaa
    25981 gttagataat aaatctaact taaggaagct agaaggatga gaatccaggt ggtcaagagt
    26041 cccaaactta agctgattgg gacacccaga ataatttgct ttttgaaggc aaggccacgt
    26101 tcctctatat tgggaagtga gagttgaatg agagaaccag ctgatgaaaa gggtgagata
    26161 ttagtagata gagcgccaat aacggtggct gttgtgagta agtgaatatc aatctgggga
    26221 ttttgagcac tgatgatagc aatgatggga aagagggctg gagctacaac ggatagggtg
    26281 gaactaaaga gtgacatcac cccggctatc acacaaaaga acagaggtaa ccagaaatga
    26341 ggaatggatg ttgtcatgag gtgccctatc agtgtgacta aacctgactt gaccgctaga
    26401 gacattagta agctcatgcc gcagagcatg ataattgtag cccagggaac tttagccaaa
    26461 atggcttctt gcttccctaa tttgagcctt aaggcgaggc agaccatgag tattgagaca
    26521 aagccaatat caaatgtttt tcgataagta gctatccagg cgatgtttgg gaaaatgaga
    26581 tgcaacaagg gaaaaagcca aaccaaaacc atgctgctga tcatgagcaa ggtggtttgt
    26641 ctttgaacct tgctgaggag tggtggttgg tcaatagtca aggatgagtt tgttcttccc
    26701 ttactatagt gactgtaaca ggataataaa agcaagacaa tgagtgggta gataatgctg
    26761 acgataaaga tatgattgcc aagtgaaaaa gcttgctctt cccatcccat ttgcttaaac
    26821 aggccttgaa agacaatgcc tgagctactg gttatcaaat tagcccctcc tgaagctccc
    26881 caattgacgg cttgagctcc aatcaaaggg tgtttgtccg ctttttgaca gagagtaatc
    26941 gctagaggac agcaaacggc catagtagtg aaaaatccag cacctaaagc agacaaaagg
    27001 gttgccatca ggtataaaat catgtagagg gcgttagggt aggtgcgtgt gcggtagaga
    27061 atgtgttgag ccaaaacatc aagagtaccg ttagttattg caaagttata aaagagagag
    27121 acgctaaaaa tggtaaaaaa gagtgaggtt ggccaaaaat gaagaagttc tttggggctt
    27181 aatcccatga gagtggttgc gatgaggtaa gaaaaagcaa tagccagcag gccaatattg
    27241 attttggtgc ggtaaccaat tccaatggct agagcaatgg cgctaatcat tattaaatga
    27301 atcattgaat tgtcctttca gttagaatat ggaaataggt tggcaatttt ggcgctcaaa
    27361 agaccacctg tttcctattg gtacttgact attatacaca aaaaaaatac aattgtcttg
    27421 acaattgtat tgacacttgt tagaattaaa aatgaggtat tcaggcttgt gatttcctat
    27481 ggaagttaat agtgattgcc tctaatgctt acaagtgata ttaaaaatag aggacctagt
    27541 gatgtcaatc atttcaactg atttaacccc ttttcaaata gatgatacat tgaaagcagc
    27601 cttgcgagaa gatgttcatt ccgaagatta cagtaccaat gccatttttg atcatcatgg
    27661 ccaagccaag gtgtcgcttt ttgccaagga agctggtgtt ttagcggggc taaccgtttt
    27721 tcaaagggtt tttaccctat ttgatgccga ggtgaccttc cagaatcctc atcaatttaa
    27781 ggatggggat cgtttgacta gtggcgattt ggttttagaa atcataggct cggtgagaag
    27841 tctcttaaca tgtgaacgcg ttgccttgaa ttttttacaa catttatcag ggattgcttc
    27901 gatgacagct gcttatgtag aagccttagg cgatgattgc attaaggtat ttgatactcg
    27961 aaaaactact cctaatttac gtctttttga gaaatatgca gtgagagttg gcggtggcta
    28021 taatcatcgc tttaatttat cagatgctat cctgctaaaa gacaatcaca ttgcggcagt
    28081 aggtagtgtt caaaaggcaa ttgctcaagc gcgtgcctat gccccttttg tgaaaatggt
    28141 cgaggtggaa gtggaaagcc ttgctgctgc cgaagaagct gcggcggcgg gtgctgatat
    28201 tatcatgttg gataatatgt cattggaaca gattgaacag gccattaccc taattgcagg
    28261 acgttctcgg attgaatgtt ctggaaatat tgatatgacc actattagcc gttttcgtgg
    28321 tttagcgatt gattacgtct ccagtggtag tttaacccat agtgctaaga gtcttgattt
    28381 ttccatgaag gggttaacct accttgatgt ctaagttgta aaataaacta actttttaaa
    28441 ggatgtcttt cctctagaac gagttttatg tcagatagtt taaacgcctc ttcaa
//
LOCUS       DAVBGY010000020        26767 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVBGY010000020 DAVBGY010000000
VERSION     DAVBGY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26767
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.020"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            205..1887
                     /locus_tag="VQ620_000939"
     CDS             205..1887
                     /locus_tag="VQ620_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2763590.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELVKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHRKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            1872..2702
                     /locus_tag="VQ620_000940"
     CDS             1872..2702
                     /locus_tag="VQ620_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2763591.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVASLLLSLQNQGRFYNPWK
                     "
     gene            2895..3398
                     /locus_tag="VQ620_000941"
     CDS             2895..3398
                     /locus_tag="VQ620_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET2763592.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      3452..3686
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3851..4414
                     /locus_tag="VQ620_000942"
     CDS             3851..4414
                     /locus_tag="VQ620_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2763593.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTVAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYILLKPTLKHYER"
     gene            4428..5054
                     /locus_tag="VQ620_000943"
     CDS             4428..5054
                     /locus_tag="VQ620_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET2763594.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRSLTRLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLTLAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGALFIEFPFYDKLRFQWRFKGKQK"
     gene            5166..6038
                     /locus_tag="VQ620_000944"
     CDS             5166..6038
                     /locus_tag="VQ620_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000525616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2763595.1"
                     /translation="MGMLLATRLKNKRKELKLSQKELAEGICEQGQISRMEQGKYSPG
                     SELLFQLSKRLKVSMDYFFEDTEVSSLENIDKFKELSKKFLDEREYESLHYIYELEKS
                     KRARLSAKDQIYLDWIDTLVQFCYLNHQTGAVAKLEKILLSLKKSDFYYLQLGNTLAN
                     FYFDMGQEDTYQELYQLLKENLQSLSVHHLEQLELMIKFRYNYCRYLWLNERTSEAIE
                     EITETIAICKRYNSSYRLADLYCLLGNISEGFAERDRILGFYQVSYQLYKHEENQRMM
                     LEIEKEMKLLESED"
     gene            6729..7643
                     /locus_tag="VQ620_000945"
     CDS             6729..7643
                     /locus_tag="VQ620_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET2763596.1"
                     /translation="MKLYQINDGIQIFESSNLEKIVIRNYRNGKKITIDHQPLLKKLL
                     LFLEVPKREEEILLYLPELSNKTLRSVLTNFMSLNIIHCQDTLIIHKKINILIIGLGT
                     TGGYLADGLCRSNINSKLFLLDKDRVDETNVGRQIYLRDDIGQYKVDVFKQKFSDKEI
                     KVFKIFINSKKDLEKICKREGIDLVIQCGDSPSPRELGKIVNTVCDTLQIPYIVNTGY
                     MSNVVPLPEFYYPNNRYNFNYKHQFSDEKLLFSQVLSKADYMVAIQSSFVMLMQIKNF
                     IFNEEPIYYGYRGYYNSVTLAWEVEKID"
     gene            7636..8649
                     /locus_tag="VQ620_000946"
     CDS             7636..8649
                     /locus_tag="VQ620_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763597.1"
                     /translation="MTKYLELDYSHSYILEGFRKNDAFIENHKECLDMLSDKIWRDNK
                     YMNTEKNISSPTRTILSRYTCNILSSLFIDISKNSIEKLIVTCENSDLNWIYNYYKSV
                     YNGVFCELRQLIQQYCKVKDKYEKWLFIKAIINQGIRQNEKEVVEFFLKQLKDNNQGI
                     FDYINSFSFYLLKFYSKDKSRIFLEEQLDIDDFLGSDKEDYARSLVFKNYASLLPDTS
                     ELKRNIMEKCLSQTPQDTDLWKEWFETYASQKEIRQKATEIFKHGYSDISLLKLVKIE
                     PDDTESLIRMIILCTSDLNSNIARYLISFLSDGRLKEFLELFVENFDFLNIIEGKTSE
                     INF"
     gene            8633..10225
                     /locus_tag="VQ620_000947"
     CDS             8633..10225
                     /locus_tag="VQ620_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2763598.1"
                     /translation="MRLIFRIIRKDIILYSILTFLLSIAMVSEAFLMQFIVDSIKFGE
                     KKYVLISIFIVIFLILQTLLYYYQQFLTAVLSKKSAYEYRKLIFKNIQQTSLRLVTGE
                     KNDSILASLTTQIDQIEYNYFYSVYWGGYLLCQLVVAIVMSLYLNPLMSVLTICLSLP
                     NLFVALLFKNSLEQKQEELINETNSSIAVTQDLIEGITDWKIANREPNIFRVFESKTL
                     KLLEKQVKVEKFQYIVVSLNQLFSNVLYFGSWIVGGFLIIRGQLSLGSLIAFSQLLAR
                     ISYPVYASSDLLSKYISGKKIVEILSKEFIFSDESRNYPDEFNVISFEHYFTNIDEKD
                     MPFDKEFYKNKKYLLKGKSGSGKTTILKSILREYDDYSGIVKINGVDVRDIKESVLFD
                     NIAYVSQKPHLFSASLRDNLTLFSEDFTDNEIVEVLKFVELSKWANKDCLDMMLSNDD
                     IKLSGGEVKRVSLARALLMNKNILLLDEFSAGIDYETLLKIEKKLIDLDKMIIYVTHV
                     DIDRSGKQFDDVIDLDCLFISS"
     gene            10417..11320
                     /locus_tag="VQ620_000948"
                     /pseudo
     CDS             10417..11320
                     /locus_tag="VQ620_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091364.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11338..11475
                     /locus_tag="VQ620_000949"
                     /pseudo
     CDS             11338..11475
                     /locus_tag="VQ620_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
     gene            11503..11844
                     /locus_tag="VQ620_000950"
     CDS             11503..11844
                     /locus_tag="VQ620_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763599.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLKNHI
                     LPLWIGMVSFF"
     gene            11817..12128
                     /locus_tag="VQ620_000951"
                     /pseudo
     CDS             11817..12128
                     /locus_tag="VQ620_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            12256..12843
                     /locus_tag="VQ620_000952"
     CDS             12256..12843
                     /locus_tag="VQ620_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HET2763600.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            13233..14840
                     /locus_tag="VQ620_000953"
     CDS             13233..14840
                     /locus_tag="VQ620_000953"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET2763601.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(15399..17030)
                     /locus_tag="VQ620_000954"
     CDS             complement(15399..17030)
                     /locus_tag="VQ620_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HET2763602.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRHAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGITITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            17323..22302
                     /locus_tag="VQ620_000955"
     CDS             17323..22302
                     /locus_tag="VQ620_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HET2763603.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLHYQGHEVSFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASSEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELTTAKQSLKDLVALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNTVLPKPTYQPTSDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            22392..23588
                     /locus_tag="VQ620_000956"
     CDS             22392..23588
                     /locus_tag="VQ620_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET2763604.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            23724..24251
                     /locus_tag="VQ620_000957"
     CDS             23724..24251
                     /locus_tag="VQ620_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET2763605.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            24328..24684
                     /gene="glnR"
                     /locus_tag="VQ620_000958"
     CDS             24328..24684
                     /gene="glnR"
                     /locus_tag="VQ620_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HET2763606.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            24721..26067
                     /gene="glnA"
                     /locus_tag="VQ620_000959"
     CDS             24721..26067
                     /gene="glnA"
                     /locus_tag="VQ620_000959"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET2763607.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(26163..26282)
                     /locus_tag="VQ620_000960"
     CDS             complement(26163..26282)
                     /locus_tag="VQ620_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET2763608.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            26540..26677
                     /locus_tag="VQ620_000961"
     CDS             26540..26677
                     /locus_tag="VQ620_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763609.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEVLNIRLEVK
                     N"
BASE COUNT         8408 a         4496 c         5709 g         8154 t
ORIGIN      
        1 actgacgtgg tttgaagaga ttttcgaaga gtataaaatg atggggaagg ctgggcaact
       61 agtctttttc gatgtttata gattagttag gcaagtaagc taggatagga atctttttgg
      121 tgctaagatt ttaagagaag agactgcaag aagggcttga tttatgataa aatagaagtc
      181 taagaagcga atgaagagag taagatgaaa gaagctataa ttgagtggaa ggatttctct
      241 ttccggtatg aaacacaaca agaaccgacc ttgcaaggga tagacttgac catttacaag
      301 ggagagaaag tcttaattgt tggaccatct ggatcaggta aatctacctt gggtcagtgt
      361 ttgaatggga ttattcccaa tatttacaag ggtcagacat atggagaatt tttgataaag
      421 ggtcaaacag cctttgatat gagcatctat gataagtctc atctggttag cacagttttg
      481 caggatacag atgggcagtt tattggcttg tctgtggcag aagatttggc gtttgctctg
      541 gaaaatgatg tgacagccct agatgagatg aaaggtcgtg tttataaatg ggctgaaaag
      601 ctggaccttc ttcctttact ggatcagcgt cctcaggatt tgtcaggtgg acaaaagcag
      661 cgagtcagtc tggctggtgt cttgattgat gaaagtccga ttctcttgtt tgatgagcca
      721 ctcgccaatc tagatcccaa gtcaggtcag gatattatcg aattgattga ccagattcat
      781 aaggaagagg ggacgacgac tcttattatc gagcaccgtt tggaggatgt tctgcatcgc
      841 cctgtggatc ggattgtctt gataaacgat ggtcgtatcc tttttaatgg gagccctgac
      901 cagttgcttg cgactgattt attgactcaa aatggaattc gagaacccct ttatctaacc
      961 actctccgtc aattaggtgt ggacttagtc aaggaagaac agttagcgaa tctggataac
     1021 atgtctatct caaaaggtca ggttcagctg cagaatgaac tggtaaaaga aactccagaa
     1081 ttgcagtcac tctttaaatt agaggacgtg tctttctctt atgatgatag accgatttta
     1141 aaatccctac atttagatat taaaaagggt gaaaagattg ctattgtcgg aaaaaatgga
     1201 gcagggaaat caactctagc caaggctata agtagcttta ttcagacgga aggacgctat
     1261 ctttgggaaa aacaggatat aaaaggcgat tctgttgcag agcgggcgga acgagtaggc
     1321 tatgtgctac aaaatcctaa tcaaatgatt tcaaccaata tgatttttga tgaggtggct
     1381 ctagggctcc gtttgcgagg tgtggatgag aaggaaattg aaacgagagt ctatgaaacc
     1441 ttgaaaatct gtggacttta tgaattccgt aattggccta tttctgccct gtcatttggt
     1501 cagaaaaaac gtgtcaccat tgcttcaatt ttggtcttag gagctgaaat tattctccta
     1561 gatgaaccga ctgcaggtca agatcagaag aactatactg agattatgga atttctcgaa
     1621 gagttacatc gaaaagggca taccattgtc atgattaccc atgatatgca attgatgctg
     1681 gattattcag accgagtcct tgtcatggtg gatggagaat tgattgccga tactgttcca
     1741 gccagtctgt tgagcgatcc tgagctgtta gtaaaagcca atctaaaaga aacctccatc
     1801 tttaacttgg ctaagaaact agatgtggat ccactggatt taacggcatt ttacaaagaa
     1861 aggagagagg gatgcaagct aaattaatcg gttaccagca tagagatact gtgattcatc
     1921 gcttgtcagg agctgggaaa cttctctttt tcattctggt atcattggcg gccatgatta
     1981 gctatgatac cagaatgctc ttgctgattg ccatcttttc agtctttctc ctctatttat
     2041 cagaaattcg ctttaaagat gtttcctttg tagccgtttt tgcgacggta tttgccgttt
     2101 taaacgtttt gatggtctat ctcttttctc ccgagtatgg ggttggactt tatggagaga
     2161 gaagtgtgat ttggcaggga atcggtgtct acactctaac cagccaggag ctcttttatc
     2221 tgctaaattt ggccattaag tatctttgca ccattcctct ggctattatc tttttgatga
     2281 caacccatcc tagtcagttt gcttccagtt taaatcaaat tggtgtgccc tataagattg
     2341 cttattctgt cagcctgacc ttgcgctata ttccagattt gcaggaagaa ttctttacta
     2401 tcaagatgtc tcaggaggcg cgtgggatgg aattatccaa gaaagcttct cttatgcaac
     2461 gaatcaaagg caatctgctc attattacgc ccttgatttt tagctcgcta gaacgcattg
     2521 ataccattgc gactgccatg gagcttcgcc gctttgggaa agaaaaaaaa cgcacatggt
     2581 atagttatca ggccttgaaa aaaggagact atattacctt gctcttggca accttgtttt
     2641 tagtagctag tttactactt agcttgcaga atcagggacg attttacaac ccttggaaat
     2701 agcttgaaaa aattgaaaaa atcaagtcgt ttctattgac aatgattctg aaagtgttat
     2761 actaagaaag tagtttcgct gatttacttc aaacctgttg tgaggtaagt taacgatgcc
     2821 ttaaccacgc tgtttgctga gcttgactcc gggcagtgtg gctatttttt tgcaatggtg
     2881 aaaggaagca agtcatgaca aatcacattg tattatttga acctcaaatt ccacaaaata
     2941 cgggcaatat cgcgcgtact tgcgctgcga ccaattctcc cctccacatc atcaagccta
     3001 tgggctttcc tattgatgac cgcaagatga agcgggctgg attggattac tgggataagc
     3061 tagagattta tttttacgag agtttggaag atttcatatc tcagatgaag ggaaaactct
     3121 atctgatttc gaaattcgcg gaaaaagtct attctgaggt ggatttatcg actgacgagg
     3181 accattattt tctctttgga cgtgaagaca agggcttacc tgaagatttt atgcgagaac
     3241 atcctgagaa ggctctccgt attcccatga atgatgaaca tgtccgcagt ctcaatgtgt
     3301 ctaataccgt ctgcatgatt gtctatgagg ctcttcgcca gcagaacttt gcaggtcttg
     3361 aactcgtcca tacctatgaa gtggataaat tgaaataaaa aatgaaaatg aacaaaatgc
     3421 ttgcgcttgc aagcgttttt tgttatgata aaagagtctt cagggcaggg tgtgattccc
     3481 gaccggcggt gactttaact aggaaatgat cttttccttt tatactttgt tgacaagctt
     3541 tgcctaacca gaagttatgc ctacagcttg tcgcctagtc taaaagaaaa atctctattt
     3601 ccttctcttt aaagaagtcc gcgagcgcaa gctgatgtgg tgagattcca caaccgacag
     3661 tatagtctgg atgggagaag acgaaagaat agctttgtct gttttgatag atttatagcc
     3721 ggattgtaac cacttgcttt gtttttcttt aatagagtag gagggaactt ttgggatata
     3781 aaaagtgaga atagatagag gaatcctttc caacttcttc tgattttata gaaaattgga
     3841 ggaacctgtt atgacaaaca cacgtcgact ttcgaccatt gcaattttat cagccatctc
     3901 atttgtgctg atgtactttg actttccgct tttaccagcg gcatctttcc tcaagatcga
     3961 atttagtatc ttgccagtcc ttgtgggtct ggtggtcatg gatttgcctg ctgctctagg
     4021 agtgctcttg cttcgctcac tcttgaaatt gcttcttaac agccaaggag tgaatactta
     4081 cattggtttg ccgatgaata tcgtagcttt gggagttttt gtcatcgtat ttgctttgat
     4141 ttggaaaaag gaacggacaa cccttcgttt cctactaggc tctctagctg ggactattgg
     4201 tttaaccgtg gctatgttag ttctcaacta tgtttatgct gttcctttgt acgctaagtt
     4261 tgctaacttt gatattggaa aaattttggg actttccaac tacctaatga ccatggtatt
     4321 accttttaac ttgattgaag gtgtaatctt ttccgtttca ttctggttgt tgtacattct
     4381 cttgaaacca accttaaaac attatgaaag ataaacaaac atttttaatg aagggcagtt
     4441 ttgccctttt acttttcgtt attcttggct atatggtcaa attttaccct gaaatgttgg
     4501 tcaattttga ccaatcgatt cagactgcca ttcgaggaga cttaccagat tatttgacta
     4561 ttctttttcg atccctcaca cgtctgattg atattccagt gattatcact tgggttgtca
     4621 ttatagcttt tgtcttttat cgtaagcgat ggaagataga aagtttcttc atgctgggaa
     4681 atctgacttt ggcaggtctt ttaatcgtga cctttaaaaa tatctaccag cgcccacgac
     4741 cagctatctt acatctggtg gaggagaagg gattttcctt cccaagtggg cattctctgg
     4801 ctgtaacgct gatggtaggg actttgattg ttattcttag tcagcggatt aaagatcagg
     4861 tctggagaaa aatcgtacaa atcgtccttg gcctctacct agtcagtgtg ctggtatcaa
     4921 gagtctatct gggagttcat tacccatcag atgttcttgc cagtctctgt gtgggcttgg
     4981 gagccctgtt tatcgagttt cccttttatg ataagctccg cttccaatgg cgatttaaag
     5041 gcaaacagaa atgatggaaa agataaaaaa ttgtacaaat acaaatagaa ccatatgaaa
     5101 tatatacttg aaacatgttt tggagtttga tatattttaa taaataaagc aaggaggaag
     5161 aaagtgtggg tatgttatta gcgacacgct taaaaaataa aagaaaggaa ttaaaattat
     5221 ctcaaaaaga attagctgag gggatttgtg agcaagggca gattagccgt atggagcaag
     5281 gtaaatactc acctgggtca gaattactct ttcaactatc taagcgattg aaggtgagca
     5341 tggattattt ctttgaagat actgaggttt ctagtttaga aaatattgat aagtttaaag
     5401 aattatctaa gaaatttttg gatgagaggg aatatgaatc tctccattac atctatgaac
     5461 tggaaaaatc taaacgtgct cgtctatctg ctaaagacca gatttatttg gattggattg
     5521 atacattagt tcaattctgt tatttaaatc atcaaacagg ggcagttgcg aaactggaaa
     5581 aaatactgtt atctctgaaa aaatctgatt tttactatct acaactggga aataccttgg
     5641 ctaattttta ttttgatatg gggcaggaag acacatatca ggaactctat caacttttaa
     5701 aagagaacct ccagtcacta tctgttcatc atttggaaca gttagaattg atgattaaat
     5761 tccgatataa ctattgtaga tatttgtggt tgaatgaacg aacatctgaa gctatagaag
     5821 aaattacaga aacgatagct atttgtaaaa gatataatag tagttatcga ttagcagatt
     5881 tgtattgttt attgggaaat ataagcgagg gctttgctga aagggatagg atactaggtt
     5941 tttatcaagt atcttaccag ctttacaagc acgaagagaa ccagagaatg atgctagaaa
     6001 ttgagaagga aatgaaatta ttggaaagtg aagattaagc aaaaagattt gtattcttag
     6061 ataactttaa tacatattat aaaaaaaata aaaaaatatc ataaaaatta ttgacaaacc
     6121 aaaattgatg tgttagaata tcaccaatag ttaaggaggt ttagaagata aaaagatctc
     6181 aagttgtcaa acgatgtata ttaattgctt tattgcttct atttctactt aatgtgttaa
     6241 gtggttcttt atctgtaaca actagaggag ttgatccaag accagcatat ggagtttcgc
     6301 tttaaagttg tgcatagttc ttcttttata gacggagagt ttttctcaat agtaagtgat
     6361 tcaaattttg atggatgatt ttccattaaa ttaagtgatt gcgaaaactt tttgaaatac
     6421 attctctttg gatattgaaa gtttgttttg gattttttta tgaagcttat cttagctgtt
     6481 agattggggt tttatgggat ttgtcctcac cgggaaaaaa tcttaaatct ttgaagaaag
     6541 aggtttttat cttatggtta gagatacatc agaagaaatt gaagttattg acttgtcttc
     6601 tatgaccgtt tcctactcat ctgttatgct aggaaactct tggatgacgt ggaattgatt
     6661 tattaagata gattatttgg tttttaccaa aaatctgtct ttttaatttt taaaggacta
     6721 atggtgatat gaaactatat caaataaatg atgggataca aatttttgaa agtagtaatc
     6781 ttgaaaaaat agtaattaga aattatagga atggtaagaa aataactatc gatcaccaac
     6841 cattattaaa aaaattacta ctattccttg aagttcctaa acgtgaagaa gaaattctat
     6901 tatatcttcc agaattatca aataaaacat tacgaagtgt tttgacgaat tttatgagtt
     6961 taaatataat tcattgtcaa gatacattaa taattcacaa aaaaataaac atattgataa
     7021 taggtttagg gactacgggt ggatatctgg cagatgggtt atgtcgtagt aatattaatt
     7081 cgaagctatt tcttttagat aaggatcgtg tagatgagac taatgtaggt agacagattt
     7141 atttaagaga tgatattggt caatataaag ttgatgtatt taaacaaaag tttagtgata
     7201 aagaaataaa ggtattcaaa atttttatta atagtaagaa agatttggaa aagatttgca
     7261 aaagggaggg tatcgatttg gttatacaat gtggtgactc cccttcacct cgagaattag
     7321 gaaaaattgt gaatacagta tgtgatactc tccagatacc ttatatagtt aacactggct
     7381 acatgagtaa tgtagttcct ttgccagaat tttattatcc aaataataga tacaatttta
     7441 attataagca tcaattttca gatgaaaagt tactgttctc acaagttttg tctaaagctg
     7501 attatatggt ggcaattcaa tcatcatttg taatgttaat gcaaataaaa aattttatat
     7561 ttaatgaaga acctatttat tatggttata ggggatacta taattctgta acattagcat
     7621 gggaggtgga aaaaattgac taaatattta gaattggatt attctcattc atatattttg
     7681 gaaggtttta gaaaaaatga tgcgtttatt gaaaatcaca aagaatgcct tgatatgtta
     7741 tcagataaga tttggcgaga taataaatat atgaatacag aaaaaaacat atcatcaccg
     7801 acacgaacaa tcttatcacg ctatacttgt aatattttat cgtctttgtt tattgatatt
     7861 agcaaaaata gcatagaaaa attgatagtg acatgtgaaa atagcgatct taattggatt
     7921 tataattatt ataaatcggt ttacaatgga gtattttgtg aattgcgaca acttatacaa
     7981 cagtactgta aggtgaagga taagtatgaa aagtggcttt ttataaaagc tattataaat
     8041 caaggtattc gtcaaaatga gaaagaagtt gttgaattct ttttaaaaca attgaaggat
     8101 aataatcaag gtatttttga ttacatcaat agttttagtt tttatctatt aaagttttat
     8161 tcaaaagaca aatctcgcat ttttttagaa gagcagctag atatagatga ttttttggga
     8221 tcagataaag aagattatgc taggtcttta gtttttaaga attatgcttc tctattgcct
     8281 gatacttctg aattaaaaag aaatattatg gaaaaatgtt taagtcaaac acctcaagat
     8341 acagatttat ggaaagaatg gtttgaaact tatgccagtc agaaagagat tagacaaaaa
     8401 gcaacggaaa tatttaagca tggttattca gatatttcgt tactaaaatt agttaaaatt
     8461 gagcctgatg acactgagag tttaatcaga atgattatct tatgtaccag tgacctaaat
     8521 agtaatattg caaggtattt gatttcattt ttgagcgatg ggcgattaaa agaatttttg
     8581 gagttatttg ttgaaaattt tgactttttg aatattattg agggaaaaac tagtgagatt
     8641 aatttttaga ataattagaa aagatattat attatattct atattaacat ttttgctatc
     8701 aatagcgatg gtaagcgaag catttttgat gcaatttatt gttgattcaa ttaaatttgg
     8761 tgaaaagaag tatgttttga tttctatttt tattgtaatt tttttgattc ttcagacttt
     8821 attgtattac tatcaacaat ttcttacagc tgtattaagt aaaaaatctg catatgagta
     8881 ccgtaaatta atttttaaaa atattcagca gacttctctt aggcttgtta caggtgaaaa
     8941 aaatgatagt attctagctt ctttgacaac gcaaatcgat caaatagaat ataattattt
     9001 ttattctgtt tattggggtg gatatttgct ttgccaatta gtggtagcta tagttatgtc
     9061 gctttatcta aatccattga tgtctgtatt aaccatttgc ttaagtctcc ctaatttatt
     9121 tgtagcactt ctttttaaaa actccttgga acaaaagcaa gaagagctaa ttaatgagac
     9181 aaattcctca atagcagtta ctcaagattt aattgaggga attactgatt ggaaaattgc
     9241 aaatagagaa cctaatatat ttagagtgtt tgaaagcaaa acattgaagc ttttagagaa
     9301 gcaagtgaag gtagaaaaat ttcaatacat tgttgttagt ttaaatcaat tgttttcaaa
     9361 tgttttatat tttggtagtt ggatagtagg tggattcttg attatacggg ggcaactttc
     9421 attagggagt ttgatagcgt tctctcaatt attagcaaga atttcttacc ctgtttacgc
     9481 ttcttcggat ttattgtcaa aatatattag cggtaaaaaa attgtagaaa ttctttctaa
     9541 agaatttata ttttcagacg aaagcagaaa ttatccagat gagtttaatg ttatttcatt
     9601 tgaacattat tttacaaaca ttgacgaaaa ggatatgcct tttgataaag aattttacaa
     9661 aaataaaaaa tatttgttga aagggaaaag tggtagtggg aaaacaacaa ttttaaaatc
     9721 gattttacga gagtatgatg attatagtgg aattgtaaaa attaatgggg tagatgttag
     9781 ggatataaaa gagagtgttc tttttgacaa tatagcttat gtttcccaaa aacctcactt
     9841 gttcagcgca agtcttagag acaacctgac attattttct gaagacttca ctgataatga
     9901 gatagttgag gttttaaaat ttgttgaatt atctaaatgg gctaataaag attgcttgga
     9961 tatgatgctt tcaaatgatg atataaaatt atcaggaggg gaggtcaaaa gggtttcttt
    10021 agctcgagcc ttattgatga ataagaatat tctgttattg gatgagttct ctgctgggat
    10081 tgattatgaa acattgttaa aaatagagaa gaaactcatt gatttggata agatgattat
    10141 ttacgtgacg catgtggata tagatagatc cggcaaacag tttgatgatg tcattgattt
    10201 ggattgtctt tttatatcct cgtgacatga gagttgagtc agttgaatta ttttgtcact
    10261 ctgtttagtt cttgttggta gtgtcaggta atctttgtta atttttcagg aagtctattg
    10321 aaatatcctc ttggttatat attacgagga gtaattaatg gatattctcg agaattcaat
    10381 acaacaattt gattctaaag ttaagtatgt ttctttattt gcggaatcaa tcttttatca
    10441 gataccatga gctattttgc aggtgctatg atttctcctt tgtatgtgag gattatcgga
    10501 gatgatatga cagatgctat gggggttaga caggcaactg ctggtgtagt taatattctc
    10561 agcaacctta tagggggagt tttgattggg ggtcatcagt cttcagatgt ttgtaggttt
    10621 gaatgctttg acctttcttt ttgcactgtt gggtgtggtg tttattcgta cttctttgag
    10681 agatattgag gaacagatgg aggttagtaa aagattagcc gcaaaatctt ttggagagca
    10741 tttcctccat tcaatgaaga tattgtcaag ttttccagcc atcatcaaat taatattcat
    10801 tgcagctatg aaccaagtga ttttaagcat tattatccct ttaacgactt tgatgttaat
    10861 ccatcatcca tttatcttct ttgacactgg gaaatccatt gcagcattgt cagtggttat
    10921 tttttcagga acgattgtgg gaagtttttt gagtggaggt atcctaaaaa atattcccat
    10981 aaaaatgatt gtctactttg aagaaattat gcatctctgt attctctttt cttggttgac
    11041 caatcatttc ctccttttat tggtagttgt cttcttatgc gcagttgcag taggaatgat
    11101 ggggcctcgg attttcaacc ttgtattctc gatggttcca gaagaagtca tgggaaccat
    11161 ccagtcagtg cttggagtat tcaatgttgt tgtaccagga attcttagca tgctcatcgt
    11221 tgccttagcg agtgctacaa acctaaaaat cacagtgctt ccacttttta tttttgtatt
    11281 gactgcctta gcaattgtga aagcaatgaa aatagagtaa tagtagtatg aactactgtc
    11341 tctttttggg tgggagttca ttacccatca gacgtccttg ccagtctctg tgtgggcttg
    11401 ggagttttgt ttatcgagtt tcccttctat gacaagctcc gcttccaatg gcgatttaaa
    11461 ggcaaacaga agtgagtatc gaattccctt gaggagaaag aaatgaaagt caatatagca
    11521 gatcttcatc cgactcaact atatttatca gaaaagaagt tgcaagatat tcagatgctt
    11581 taccagtcgg cagaaacaat ccaagtcgat ccaatcagta ttcttgcctt tggagattgt
    11641 ttgctgatta cagatgggca tcacagggct tatcaggctt tattggcagg tcgggatacg
    11701 atttctgctg agtgggatag agatggtggt gatgaactat atcatctcta tgcgcaagct
    11761 tgcgaggaaa gaaagattta ctctgttttt gatttaaaaa atcatatctt acctctttgg
    11821 atcggcatgg tgagcttttt ttgattgaag tcaaggagaa gggagaatgg tttccaattg
    11881 gggatattac actatctcag gataatctcc ccattgtgat tgggaattcc gcttaccaac
    11941 atcgaggact tggaaaaaag attctaagtg ctttgattga attggctcga gtaaaaggat
    12001 ggaaagaatt gagagtcaag gaaatctaca cctacaatca tgcttctagg aggtgtttca
    12061 agtcgcttgg atttgtggaa aatggagcaa cagaaaaagg aaggagtttt atattggaat
    12121 tagtctaatc ctgccacttt ctccccctta acctttgact attccgcaaa attatcgtaa
    12181 aataaagagt aaatgataaa atgaggtcag agtctgttcg ctctgacgat agtagtataa
    12241 atgaggagaa acgctttgga attagaagta tttgctgggc aagaaaaaag tgaactatct
    12301 atgattgagg tagcgcgtgc tatattagaa cttcgtggtc gcgatcatga gatgcatttt
    12361 agcgatcttg taaacgaaat tcaaaactac cttggaacat caaacagcga tatccgcgaa
    12421 gctttgcctc tgttctacac agagttgaac tttgacggta gcttcatctc acttggggac
    12481 aacaaatggg gtcttcgttc atggtatggt gtggacgaaa tcgacgaaga aatcatcgct
    12541 cttgaagaaa atgacgacga tgaagtagca ccaaaagcta agaaaaaacg tgtcaatgcc
    12601 tttatggatg gtgattcaga tgccattgac tacaatgcag atgatccaga agacgaagat
    12661 gcatacgaag cagatccagc tctttcatac gatgatgaaa atccagatga tgaaaaaaat
    12721 gaagtggaag cttatgatgc agaaatcaac gaaatcgctc cagatgactt gggagaagat
    12781 gtggatctca acgaagacga cgacgagttt tcagatgacg acgctgaaac cagcgaggaa
    12841 taagagttag ctattgacaa ttatcctatt tttaggtatc atattgtttg ggcacctctt
    12901 ttagaggtcg gggctcccta gttcttaggg agctattttt gttttttcaa gaagttatct
    12961 tcttgtattt tatactcaat gaaaatcaaa gagcaagcta ggaaactagc cgtaggctgc
    13021 tcaaaacact gttttgaggt tgtagataag actgacaaag tcaggaacac atatctacgg
    13081 caaggcgacg ttgacgcggt ttgaagagat tttcgaagag tattagttgt gaatctggtg
    13141 cagtcgtccc agattattct tattagtagg gtcttgtttt ctatatcccc tcgtagttaa
    13201 caagaccttg agcattttag aaagaggaat ctatgtctac gaaatatatt tttgtaactg
    13261 gtggtgtggt atcgtctatt gggaaaggga ttgtggcagc gagtctaggc cgtctcttga
    13321 aaaatcgtgg tctcaaagta accattcaaa agtttgaccc ttatatcaat attgatccgg
    13381 gaaccatgag tccttaccag cacggggaag tttttgtgac agatgacgga gctgagacag
    13441 atttggactt gggtcactat gaacgtttca tcgatatcaa tctcaacaaa tattccaacg
    13501 tgacaactgg gaaaatttac agtgaagttc ttcgtaaaga acgccgtgga gaataccttg
    13561 gggcaactgt tcaagtcatt cctcatatca cagatgcttt gaaagaaaaa atcaagcgtg
    13621 ccgctctaac gaccgactct gatgtcatta tcacagaggt tggtggaaca gtaggagata
    13681 tcgagtcctt gccattccta gaggctcttc gtcagatgaa ggcagatgtg ggtgcggata
    13741 atgtcatgta tatccataca accttgcttc cttacctcaa ggctgctggt gaaatgaaaa
    13801 ccaaaccaac ccaacactct gtcaaagaat tgcgtggctt gggaatccaa ccaaatatgt
    13861 tggttattcg tacagaagag ccagctggtc aaggaattaa aaataaactg gcccagttct
    13921 gtgatgtggc accagaagcc gttatcgaat cgttggatgt tgaacacctt taccaaattc
    13981 cactgaactt gcaggcacaa gggatggacc aaattgtttg tgatcatttg aaattagacg
    14041 caccagcagc ggatatgaca gaatggtcag ccatggtgga caaggtcatg aacctcaaga
    14101 aacaagttaa gatttccctt gttggtaagt atgtggagtt gcaagatgcc tatatctcag
    14161 tggtcgaagc cttgaaacac tctggctatg tcaatgatgt agaagttaaa atcaattggg
    14221 tcaatgccaa tgatgtgaca gcagagaatg tagcagaact cttgtctgat gcggacggga
    14281 tcatcgtacc aggtggtttt ggtcaacgtg gtacagaagg gaaaatccaa gccatccgct
    14341 atgcgcgtga aaatgatgtt ccaatgttgg gagtctgctt gggaatgcag ttgacatgta
    14401 tcgagtttgc tcgtcacgtt ttaggtcttg aaggtgccaa ttctgcagag cttgcaccag
    14461 aaacaaaata ccctatcatt gatatcatgc gtgatcagat tgatattgag gatatgggtg
    14521 gaacccttcg tttgggactt tatccgtcta agttgaaacg tggctctaag gctgctgctg
    14581 cttatcacaa tcaagaagtg gtgcaacgcc gtcaccgtca ccgttatgag tttaataatg
    14641 ccttccgtga gcagtttgag gcagcaggtt ttgtcttttc aggagtttct ccagacaatc
    14701 gtttggtaga aatcgtggaa attcctgaaa ataaattctt tgtagcttgt cagtatcacc
    14761 ctgaactgtc aagccgtcca aaccgaccag aagaactcta cactgccttt gttactgcag
    14821 cggttgagaa cagcaattag caaaatcaga acctttgaga aaaatctcag aggttttttg
    14881 catacgatga tattgcagta tatctgaggt agggggcctc tgtatgtacc tgctaccgtt
    14941 gaaatcaata gcgactccct cttgccctgt gctagtgaat ggatttatca gtatattgaa
    15001 atgaaataaa atttgaacaa attaattcgg aaagccaaat caatttctag caaagtttta
    15061 ggaactggat tgtatagtga attgaaataa gatgtgaaca tctctatcag aaaagtcaaa
    15121 ttaatttata gaaatatttt agcagtcaag atgtactgtt atagattcaa tacattatac
    15181 ttttttaatt taatccacta tagtaaaatg aaataagaac aggacaaatc gatcaggaca
    15241 gtcaaatcga tttctaacaa tgttttagaa atagaggtgt actattctag tttcaatata
    15301 ctatatatct gcagaatact ctttcagttt cattcagcct agtcttgctt aggaaaaagt
    15361 aaaaagccat ccaattatga tggatagctt cttggttctt agatttgttc tgcaaaaacc
    15421 ttttcagcaa ggttcatagc gtggtctgat acacgagtgt agtgtgagat gatgtcgata
    15481 aagttgaccc cagcttgtgt tgaacattcg cctttgttga ggcgtttgat gtgggttttt
    15541 ctgagaacac gttctatctt attgattgct tcatgacgtt ctacaagact gcgtgctttt
    15601 tcaatatcat tgttttccac actatccaga gcatctttga taaagtcact agtttggcgg
    15661 taaacttcct ctaattcttt caaggcggca tcagaaaatt caacattttt ccgttgaaga
    15721 tagtcagtca gattgagtag agcctccgtg tggtctccaa tccgttccaa atcacgggag
    15781 gaatcaagga tattggtaag cacttcactt tctttttggc tgagagcttc gcttgaaagg
    15841 gcaatgagat aacgtgttaa ttgctcatcg atggtgttaa ttgcttcttc ggttttatgc
    15901 cctttttcag caactttttc atccaagtca atgatgtact tataggaaag gtcaaaggct
    15961 ttagcagcgt agtttcctaa gtgcaagagc tctttcttag catttcctag agcgatagat
    16021 ggggcctgtt tgatgaaatg ttcatcaaga tataagggtt cgtatttgac aacctcgtcc
    16081 tctccaggaa taatcttggt tacaaagtaa gccagagctc cgataaatgg aaattggaca
    16141 atggtgttgg taatattaaa ggttccgtga gcaaaggcga tggtcatttc cggtgctaga
    16201 tttagcgtag cttcaaacca atgaatcagg acagtaaaag gaactagaaa aataacgcag
    16261 acgactgttc cgataacgtt gaaggcaaca tgagctcctg ctacccgttt agctgcaata
    16321 ttagccccta aagaggcaat gatggctgta atggttatcc cgatattgtc accaaataga
    16381 actggcaaag ctccctgtag gtcaattaga ttgccggcgt agaggttttg taaaatccca
    16441 atggtagccg aagaagcttg aattagcaag gtcaagccgg taccgacaaa gacacccaaa
    16501 acaggattct tacttagctc aatcatatag tccttaaaga cctgtaaatc cttgagtgga
    16561 gccattgcgc cgctcatgag attgagggca aaaaagatac caccgacacc aaagaggatg
    16621 cgtccgatat tattgactgt ccgatttttt gtaaaaaaga gacagacggc accgataaag
    16681 agcataggta gggcatagtt acctaattta aaaccgatga gaaaggatgt gacagttgtc
    16741 ccaatattag cacccatgac aatcccgata gcatgacgta aggttaagag accggcactg
    16801 accaggccga ctgtgataac tgttacacca gaactagact gaattagagc agtcatccca
    16861 ataccaacca gaactccaaa gaaaggatta ctagtatatt tgtcaatgta aaaacgaagg
    16921 cgatctccag cagcttgttg caaaccgtct cccatggtct tgatgctata taagaatagc
    16981 cccagaccac ctaaaaagtg aaataaaatt tcctgccaat taatggacat ttctttttcc
    17041 tccgaaaaat aatcgcggaa tatctcctat tctattttaa aggataaaag taaatctaac
    17101 aagtgttaat gtcaagtttg taaaagaaaa ttataattga taagtgtatt atttttaaga
    17161 gtttttaacc ttattttaga tttgtgcaaa cagttattgt tttttgtcta aaaatgtttt
    17221 aggataaaaa tgtgattatc atggttctag agtttgataa aaaactacag aatataaagc
    17281 gctttcttga aaacaacaaa atcaatcttt taggaggaga aaatgaagaa tccatttttt
    17341 gaaagacgtt gtcgttacag tattcgtaag ttatcagtag gagcctgctc gctgatgatt
    17401 ggtgctgttt tatttgctgg tccagccttg gctgaagaaa ctgcagttcc tgaaaatagc
    17461 ggagctaata cagagcttgt ttcaggagag agtgagcatt cgaccaatga agctgataag
    17521 cagaatgaag gggaacatgc tagagaaaac aagctagaaa aggcagaagg agtagcgata
    17581 gcatctgaaa ctgcttcgcc agcaagcaat gaagctgcaa ctactgaaac tgcagaagca
    17641 gctagcgcag ctaaaccaga ggaaaaagca agtgaggtgg ttgcagaaac accatctgca
    17701 gaagcaaaac ctaagtctga caaggaaaca gaagcaaagc ccgaagcaac taaccaaggg
    17761 gatgagtcta aaccagcagc agaagctaat aagactgaaa aagaagtcca gccagatgtc
    17821 cctaaaaata cagaaaaaac attaaaacca aaggaaatca aatttaattc atgggaagaa
    17881 ttgttaaaat gggaaccagg tgctcgtgaa gatgatgcta ttaaccgcgg atctgttgtc
    17941 ctcgcttcac gtcggacagg tcatttagtc aatgaaaaag ctagcaagga agcaaaagtt
    18001 caagccttat caaacaccaa ttctaaagca aaagaccatg cttctgttgg tggagaagag
    18061 ttcaaggcct atgcttttga ctattggcaa tatctagatt caatggtctt ctgggaaggt
    18121 ctcgtaccaa ctcctgacgt tattgatgca ggtcaccgta acggggttcc tgtatacggt
    18181 acactcttct tcaactggtc taatagtatt gcagatcaag aaagatttgc tgaagctttg
    18241 aagcaagacg cagatggtag cttcccaatt gcccgtaaat tggtagacat ggcaaagtat
    18301 tatggctatg atggctattt catcaaccaa gaaacaactg gagatttggt taaacctctt
    18361 ggagaaaaga tgcgccagtt tatgctctat agcaaggaat atgctgctaa ggtaaaccat
    18421 ccaatcaagt attcttggta tgatgccatg acctataact atggacgtta ccatcaagat
    18481 ggtttgggag aatacaacta ccaattcatg caaccagaag gagataaggt tccggcagat
    18541 aacttctttg ctaactttaa ctgggataag gctaaaaatg attacactat tgcaactgcc
    18601 aactggattg gtcgtaatcc ttatgatgta tttgcaggtt tggaattgca acagggtggt
    18661 tcctacaaga caaaggttaa gtggaatgac attttagacg aaaatgggaa attgcgcctt
    18721 tctcttggtt tatttgcccc agataccatt acaagtttag gaaaaactgg tgaagattat
    18781 cataaaaatg aagatatctt ctttacaggt tatcaaggag accccactgg ccaaaaacca
    18841 ggtgacaaag attggtatgg tattgctaac ctagttgcgg accgtacgcc agcggtaggt
    18901 aatactttta ctacttcttt taatacaggt catggtaaaa aatggttcgt agatggtaag
    18961 gtttctaagg attctgagtg gaattatcgt tcagtatcag gtgttcttcc aacatggcgc
    19021 tggtggcaga cttcaacagg ggaaaaactt cgtgcagaat atgattttac agatgcctat
    19081 aatggcggaa attcccttaa attctctggt gatgtagctg gtaagacaga tcaggatgtg
    19141 agactttatt ctactaagtt agaagtaact gagaagacca aacttcgtgt tgcccacaag
    19201 ggaggaaaag gttctaaagt ttatatggca ttctctacaa ctccagacta caaattcgat
    19261 gatgcagatg catggaaaga gctaaccctt tctgacaact ggacaaatga agaatttgat
    19321 ctcagctcac tagcgggtaa aaccatctat gcagtcaaac tatttttcga gcatgaaggt
    19381 gctgtaaaag attatcagtt caacctcgga caattaacta tctcggacaa tcaccaagag
    19441 ccacaatcgc cgacaagctt ttctgtagtg aaacaatctc ttaaaaatgc ccaagaagcg
    19501 gaagcagttg tgcaatttaa aggcaacaag gatgcagatt tctatgaagt ttatgaaaaa
    19561 gatggagaca gctggaaatt actaactggc tcatcttcta caactattta tctaccaaaa
    19621 gttagccgct cagcaagtgc tcagggtaca actcaagaac tgaaggttgt agcagtcggt
    19681 aaaaatggag ttcgttcaga agctgcaacc acaacctttg attggggtat gactgtaaaa
    19741 gataccagcc taccaaaacc gctagctgaa aatatcgttc caggtgcaac agttattgat
    19801 agtactttcc ctaagactga aggtggagaa ggtattgaag gtatgttgaa cggtaccatt
    19861 actagcttgt cagataaatg gtcttcagct cagttgagtg gtagtgtgga tattcgtttg
    19921 accaagccac gtaccgttgt tagatgggtc atggatcatg caggggctgg tggtgagtct
    19981 gttaacgatg gcttgatgaa caccaaagac tttgaccttt attataaaga tgcagatggt
    20041 gagtggaagc tagctaagga agtccgtggt aacaaagcac acgtgacaga tatcactctt
    20101 gataaaccaa tcactgctca agactggcgc ttgaatgttg tcacttctga caatggaact
    20161 ccatggaagg ctattcgtat ctataactgg aaaatgtatg aaaagcttga tactgagagt
    20221 gtcaatattc cgatggccaa ggctgcagcc cgttctctag gcaataacaa ggtacaagtt
    20281 ggctttgcag atgtaccggc tggagcaact attaccgttt atgataatcc aaattctcaa
    20341 actccgctcg caaccttgaa gagcgaagtt ggaggagacc tagcaagtgc accattggat
    20401 ttgacaaatc aatctggtct tctttattat cgtacccagt tgccaggcaa ggaaattagt
    20461 aatgtcctag cagtttccgt tccaaaagat gacagaagaa tcaagtcagt cagcctagaa
    20521 acaggaccta agaaaacaag ctacgccgaa ggggaggatt tggaccttag aggtggtgtt
    20581 cttcgagttc agtatgaagg aggaactgag gacgaactca ttcgcctaac tcacgcaggt
    20641 gtatcagtat caggttttga tacgcatcat aagggagaac agaatcttac tctccaatat
    20701 ttgggacaac cggtaaatgc taatttgtca gtgactgtca ctggccaaga cgaagcaagt
    20761 ccgaaaacta ttttgggaat tgaagtaagt caggaaccga aaaaagatta cctagttggt
    20821 gatagcttag acttgtctga aggacgcttt gcagtggctt atagcaatga caccatggaa
    20881 gaacattcct ttactgatga gggagttgaa atttctggtt acgatgctca aaagactggt
    20941 cgtcaaacct tgacgcttca ttaccaaggc catgaagtta gctttgatgt tttggtatct
    21001 ccaaaagcag cattgaacga tgagtacctc aaacaaaaat tagcagaagt tgaagctgct
    21061 aagaacaagg tggtctataa ctttgcttca tcagaagtaa aagaagcctt cttgaaagca
    21121 attgaagcgg ccgaacaagt gttgaaagac catgaaacta gcacccaaga tcaagtcaat
    21181 gaccgactta ataaattgac agaagctcat aaagctctga atggtcaaga gaaatttacg
    21241 gaagaaaaga cagagcttga tcgcttaaca ggtgaggttc aagaactctt ggctgccaaa
    21301 ccaaaccatc cttcaggttc tgccctagct ccgcttcttg agaaaaacaa ggccttggtt
    21361 gaaaaagtag atttgagtcc agaagagctt acaacagcga aacagagtct aaaagatctg
    21421 gttgctttat tgaaagaaga caagccagca gtcttttctg atagtaaaac aggtgttgaa
    21481 gtacacttct caaataaaga gaagactgtc atcaagggtt tgaaagtaga gcgtgttcaa
    21541 gcaagtgctg aagagaagaa atactttgct ggagaagatg ctcatgtctt tgaaatagaa
    21601 ggtttggatg aaaaaggtca agatgttgat ctctcttatg cttctattgt gaaaatccca
    21661 attgaaaaag ataagaaagt taagaaagta tttttcttac ctgaaggcaa agaggcagta
    21721 gaattggctt ttgaacaaac ggatagtcat gttatcttta cagcacctca ctttactcat
    21781 tatgcctttg tttatgaatc tgctgaaaaa ccacaacctg ctaaaccagc accacaaaac
    21841 acagtccttc caaaacctac ttatcaaccg acttctgatc aacaaaaggc tcctaaattg
    21901 gaagttcaag aggaaaaggt tgcctttcat cgtcaagagc atgaaaatgc tgagatgcta
    21961 gttggggaac aacgagtcat catacaggga cgagatggac tgttaagaca tgtctttgaa
    22021 gttgatgaaa acggtcagcg tcgtcttcgt tcaacagaag tcatccaaga agcgattcca
    22081 gaaattgttg aaattggaac aaaagtaaaa acagtaccag cagtagtagc tacacaggaa
    22141 aaaccagctc aaaatacagc agttaaatca gaagaagcaa gcaaacaatt gccaaataca
    22201 ggaacagctg atgctaatga agccctaata gcaggcttag ccagccttgg tcttgctagt
    22261 ttagccttga ccttgagacg gaaaagagaa gataaagatt aaatatcgaa aaatcttgtg
    22321 aaatctttcc ttatatttcc aaagtgtgat ataatagttt cgaataaaat aaataaagga
    22381 gtttttgtaa catggcaaaa cttactgtta aagacgttga cttgaaaggt aaaaaagtcc
    22441 tcgttcgtgt tgacttcaac gtaccattga aagatggcgt aatcactaac gataaccgta
    22501 tcacagcagc tcttccaact attaagtaca tcatcgaaca aggtggacgt gcaattcttt
    22561 tctctcacct tggacgtgtg aaagaagaag ctgataaagc tggtaaatca cttgctcctg
    22621 tagcagctga cttggcagca aaacttggtc aagatgttgt tttcccaggt gtcactcgtg
    22681 gtgctgaatt agaagcggca atcaacgctc ttgaagatgg acaagttctc ttggttgaaa
    22741 acactcgtta cgaagatgtt gacggcaaga aagaatctaa aaacgatcct gaacttggta
    22801 aatactgggc atcacttgga gatggtatct tcgtaaacga tgcattcggt acagctcacc
    22861 gtgcacacgc atctaatgtt ggtatctcag caaacgttga aaaagcagtt gctggtttcc
    22921 ttcttgaaaa cgaaattgcc tacatccaag aagcagttga aactccagaa cgtccattcg
    22981 tggctatcct tggtggttca aaagtttcag acaagatcgg tgttatcgaa aacttgcttg
    23041 aaaaagctga taaagtcctt atcggtggtg ggatgactta cacattctac aaagcacaag
    23101 gtatcgaaat tggtaactca cttgtagaag aagacaaatt ggatgttgcg aaagctcttc
    23161 ttgaaaaagc aaatggtaaa ttgatcttgc cagttgactc aaaagaagct aacgcatttg
    23221 ctggttacac tgaagtgcgt gacactgaag gtgaagcagt ttctgaaggc ttccttggtc
    23281 ttgacatcgg tccaaaatct atcgccaaat ttgacgaagc tttgactggt gccaaaacag
    23341 ttgtatggaa cggacctatg ggtgtatttg aaaacccaga tttccaagct ggtacaatcg
    23401 gtgtgatgga cgctatcgtg aaacaaccag gagttaaatc aatcatcggt ggtggtgact
    23461 cagctgccgc agcgattaac cttggccgtg cagacaagtt ctcatggatt agtacgggtg
    23521 gtggagcatc aatggaactt cttgaaggta aggttcttcc aggacttgca gccctgacag
    23581 aaaaataaga ttttataaac aaatcaaaga agagagggat gaaagttcct cttttctttt
    23641 gcttaaaata aaaacgcttc ctctcaacta ttactcataa aaatcaccga tttatgataa
    23701 aatggaaata gaaagttgag attatgagtt attttaaaaa atataaattc gataaatccc
    23761 agttcaaact tggtatgcga acctttaaaa caggtattgc tgtttttcta gttctcttga
    23821 tttttggctt ttttggctgg aaaggtcttc aaattggtgc tttgacagcc gtttttagcc
    23881 tgagggagag ttttgatgag agtgttcatt ttgggacttc gcgtattcta ggaaatagta
    23941 tcggtggact ctatgccttg gtctttttct tattaaatac ctttttccac gaagcctttt
    24001 gggtgacctt ggtagttgtt ccaatctgca ccatgttaac cattatgaca aatgtagcca
    24061 tgaataacaa agcaggggtt attggtggtg tagcagctat gttaatcatt accctatcaa
    24121 ttccaagtgg tgagacaatt ttgtacgtgt ttgtgcgtgt attagaaacg tttatgggag
    24181 tttttgtcgc aattatcgta aattacgata ttgatcgtat tcgtctcttt ttagagaaaa
    24241 aagaaaaata atgttacatt ttataacatt atcaattgac gtttgtcttt ttttagacta
    24301 taatagacag aaagaaggaa attgtaaatg aaggaaaaag aatttcgccg aaatatggct
    24361 gtttttccta tcggcagtgt tatgaagttg accgatttat cggcgcgtca gattcgttat
    24421 tatgaagatc aagagttgat caagcccgat cgaaacgaag gaaatcgtcg catgtattcc
    24481 ttgaatgaca tggatcgtct gcttgaaatc aaagattata tctctgaagg ttataatatc
    24541 gctgccatta agaaaaaata tgctgaacgt gaagcgaaat ccaagaaagc ggttagtcag
    24601 actgaagtac gtcgtgcact tcacaatgaa ctcctccaac aggggcgttt tgcttcagta
    24661 cagtcacctt ttggtcgcgg ttaggcaacc gcaagtagtc atagattaag gagaaaactt
    24721 atgccaatca cagctgcaga tattcgtcgt gaagtcaagg aaaaaaatgt tacctttatt
    24781 cgtcttatgt tctcagatat tttgggaacc atgaaaaacg tcgaaattcc tgctacagat
    24841 gaacagttag ataaggtctt gtcgaacaag gttatgtttg atggatcttc tattgaaggt
    24901 tttgtacgta tcaatgagtc ggatatgtac ttgtacccgg acttggatac atggacagtc
    24961 ttcccttggg gagatgaaaa tggaagtgtt gcaggtctga tctgtgatgt ctatacaaca
    25021 gaaggtgaac catttgcggg tgaccctcgt ggtaatttga aacgagctct tcgtcacatg
    25081 gaagaagttg gattcaaatc cttcaacctt ggtccagagc cagaattctt cctatttaag
    25141 ttggatgaaa atggggaccc aacacttgaa gtgaatgaca agggtggcta ctttgacttg
    25201 gcacctactg atcttgcgga caacacacgt cgtgagattg tgaatgtctt gaccaaaatg
    25261 ggatttgaag tagaagcgag tcaccacgag gttgcggttg gacagcatga gattgacttt
    25321 aagtacgatg aagttctccg tgcttgtgat aagattcaaa tctttaagct tgttgttaaa
    25381 accattgctc gcaaacacgg actttacgca acatttatgg cgaagccaaa atttggtatt
    25441 gctggatcag gtatgcactg taatatgtcc ttgtttgatg cagaaggaaa taacgccttc
    25501 tttgatccaa atgatccaaa aggaatgcag ttgtcagaaa cagcttacca tttcctaggc
    25561 ggtttgatca agcatgctta caactatact gctatcatga acccaacagt taactcatac
    25621 aaacgtttgg ttccaggtta tgaagcgcct gtttacattg cttgggctgg tcgtaaccgt
    25681 tcgccacttg tgcgcgtacc tgcttcacgt ggtatgggaa ctcgtcttga gttgcgttca
    25741 gtggatccaa tggcgaaccc ttacgttgct atggctgttc ttttggaagt tggtttgtat
    25801 ggtattgaaa ataaaatcga agcaccagct cctatcgaag aaaatatcta catcatgaca
    25861 gcagaagagc gcaaggaagc tggtattaca gaccttccat caactcttca caacgctttg
    25921 aaagctttga cagaagatga agtggttaaa gctgctctcg gagatcacat ctatactagc
    25981 ttccttgaag ccaaacgaat cgaatgggca agttatgcaa ccttcgtttc acaatgggaa
    26041 attgataatt atttagacct ttactaatac taatatagaa gaaagattgc ctgtgggtaa
    26101 acagtcacac agcagtctaa gccatcaaat acctataacc cttaaaatag cgatttcggt
    26161 cgctatttat ttttcgaaaa atcctcttga ctcttttgcg aattagattt atcatcagac
    26221 gtgtagaaga aaattaagga gtcactatgg aaaaattcaa tttcaaaaac aatatcgggc
    26281 aagaaaacaa gttacttcaa atcgaaatct acaagtttac taacttttgc aagcttcaaa
    26341 attacacatc tgtaaatatt ttttcgaaag atatatttga agccatagta aattgagact
    26401 agaatagtac actacatctg ctaaaaattt ctagaaacta atttaacttt cctaatcgat
    26461 ttgtttacat cttatttcaa tctactatat tatctcaata aatgatgatg gcgactgttt
    26521 tgtttgtgct tgtcgatttt tgaatcctga tcgagcggaa gaatatttct gccgaggttg
    26581 ccaaggggaa aatcctgaag atattgaatt ttatgatgaa caattacagg cagaaaaggt
    26641 ggaggttttg aatattcgat tggaagtaaa aaattgaatt atctacaatc tcatcaacaa
    26701 taaacctatt tcaaccccat tatctatagg cgtattttta cgccttttta tatacttgaa
    26761 gcacata
//
LOCUS       DAVBGY010000021        26515 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVBGY010000021 DAVBGY010000000
VERSION     DAVBGY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26515
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.021"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(44..121)
                     /locus_tag="VQ620_000962"
                     /pseudo
     CDS             complement(44..121)
                     /locus_tag="VQ620_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(183..632)
                     /locus_tag="VQ620_000963"
     CDS             complement(183..632)
                     /locus_tag="VQ620_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET2763610.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(634..1392)
                     /locus_tag="VQ620_000964"
     CDS             complement(634..1392)
                     /locus_tag="VQ620_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET2763611.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1507..3486)
                     /gene="pknB"
                     /locus_tag="VQ620_000965"
     CDS             complement(1507..3486)
                     /gene="pknB"
                     /locus_tag="VQ620_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HET2763612.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENSSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3483..4223)
                     /locus_tag="VQ620_000966"
     CDS             complement(3483..4223)
                     /locus_tag="VQ620_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET2763613.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(4238..5551)
                     /gene="rsmB"
                     /locus_tag="VQ620_000967"
     CDS             complement(4238..5551)
                     /gene="rsmB"
                     /locus_tag="VQ620_000967"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET2763614.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5544..6479)
                     /locus_tag="VQ620_000968"
     CDS             complement(5544..6479)
                     /locus_tag="VQ620_000968"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET2763615.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMEIASFLNGVGRTLTVGERFGD"
     gene            complement(6492..8888)
                     /locus_tag="VQ620_000969"
     CDS             complement(6492..8888)
                     /locus_tag="VQ620_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET2763616.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQVRDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGGAQVVVGARSAIFAPLKNLGIMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDICPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(8954..9268)
                     /locus_tag="VQ620_000970"
     CDS             complement(8954..9268)
                     /locus_tag="VQ620_000970"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET2763617.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPEPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9293..9919)
                     /gene="gmk"
                     /locus_tag="VQ620_000971"
     CDS             complement(9293..9919)
                     /gene="gmk"
                     /locus_tag="VQ620_000971"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET2763618.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(10049..11662)
                     /locus_tag="VQ620_000972"
     CDS             complement(10049..11662)
                     /locus_tag="VQ620_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET2763619.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LECIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNAIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(12006..14753)
                     /locus_tag="VQ620_000973"
     CDS             complement(12006..14753)
                     /locus_tag="VQ620_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HET2763620.1"
                     /translation="MEENYNLSITQIKNSIKENSLVLFVGAGISANSNLPTWGELIQS
                     LKKELNIPEERTDSPLRIAQYYYDTFGKNQYTKKIEEIFFKKGLSKPNELHKLIEKIA
                     PKHIITTNYDSLLESQFESGLLKYNVVAEDKDIPYTSSERYLIKMHGDFSKKNIVLKE
                     DDYLDYHLNFPMISTLIQSLIMNHTLLFVGYSLSDSTFNSIFRMIQNTFKLDAKNAYF
                     YTPKEPSMIIREYYKKQGIFIISNEENLGQETSEKQNKLYCRTKDFLEVLSENRSQDV
                     NNADDLWNQLAFLDRLSFIDAKDFSRYSDLKKRALNWDDEYCWFGNDQLRFEIDGHEE
                     LRIMVSKKSLLNRFLDMEIGEPRDLKGNRFLSKAFKLYEEKQYSLAKAKFRELANIAF
                     RQKDYFNFLVCEFNFQQIQIIDRYEKTPSYAEPLYDGELSELTEQIINSVTGDEKKII
                     EFFRDSILNFNFLYRKLETINELFDEIREEHESYRRGGWSANNHLFNAEFTVKNLYNF
                     LKLNCLCVEHYKIYKSIINRYLEILLLSYDNSYVNPDSSIFDRTSSWLKNLDLDDVQL
                     ILPNIDFKVVNLYFRNYSFGKIKVTEEAKDYLLNRITYLQERLEITEDENLRELKNML
                     IFLPLVDDIDIEKVIEILNNQTLYYNWSEEFRRIIKIVLDNMDVIDKDSLKSKIIGIV
                     NKHLNEILEKNFSLYNSVYPLYSQLLEYCSTDQETAIIVLEKFNTDILRIKYKNDDIK
                     NIIEYSDLICHLFKYFEKDIKDDILDTLKVYEESENIIYHKVIDLMSYNVYDFPQIQN
                     KIYHYLIKRINDKRVEGVKTFPDPREKSVSDLYNLSRKGYFSDFEILKDIEEDIRGLY
                     PEVDWTWFHDRSDDVIHRLLEHRTPNNIKTYFSKNEEDNKLINEYILKAFNEDKLILK
                     K"
     gene            complement(15101..16198)
                     /locus_tag="VQ620_000974"
     CDS             complement(15101..16198)
                     /locus_tag="VQ620_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET2763621.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVLD
                     YQKIADLYTEKGAEMEKFVKKLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMVVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGNPSIAQQNFGRVPIRIERN"
     gene            complement(16208..16954)
                     /locus_tag="VQ620_000975"
     CDS             complement(16208..16954)
                     /locus_tag="VQ620_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2763622.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNVLNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(17057..17410)
                     /gene="rsfS"
                     /locus_tag="VQ620_000976"
     CDS             complement(17057..17410)
                     /gene="rsfS"
                     /locus_tag="VQ620_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET2763623.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(17423..17923)
                     /locus_tag="VQ620_000977"
     CDS             complement(17423..17923)
                     /locus_tag="VQ620_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET2763624.1"
                     /translation="MKMAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFHKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(17924..18517)
                     /gene="yqeK"
                     /locus_tag="VQ620_000978"
     CDS             complement(17924..18517)
                     /gene="yqeK"
                     /locus_tag="VQ620_000978"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HET2763625.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDFDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQILETYNAFVHYLKEN"
     gene            complement(18517..19146)
                     /locus_tag="VQ620_000979"
     CDS             complement(18517..19146)
                     /locus_tag="VQ620_000979"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET2763626.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEQHPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRAFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(19198..19509)
                     /gene="yhbY"
                     /locus_tag="VQ620_000980"
     CDS             complement(19198..19509)
                     /gene="yhbY"
                     /locus_tag="VQ620_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HET2763627.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(19723..20829)
                     /gene="yqeH"
                     /locus_tag="VQ620_000981"
     CDS             complement(19723..20829)
                     /gene="yqeH"
                     /locus_tag="VQ620_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HET2763628.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSNALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVVTRKAII"
     gene            complement(20832..21422)
                     /locus_tag="VQ620_000982"
     CDS             complement(20832..21422)
                     /locus_tag="VQ620_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HET2763629.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(21374..22282)
                     /locus_tag="VQ620_000983"
     CDS             complement(21374..22282)
                     /locus_tag="VQ620_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET2763630.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(22339..22899)
                     /locus_tag="VQ620_000984"
     CDS             complement(22339..22899)
                     /locus_tag="VQ620_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HET2763631.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            23038..24247
                     /gene="sstT"
                     /locus_tag="VQ620_000985"
                     /pseudo
     CDS             23038..24247
                     /gene="sstT"
                     /locus_tag="VQ620_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            24315..25268
                     /locus_tag="VQ620_000986"
     CDS             24315..25268
                     /locus_tag="VQ620_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET2763632.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            25411..25791
                     /locus_tag="VQ620_000987"
     CDS             25411..25791
                     /locus_tag="VQ620_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2763633.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEKTIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKILASLQKTVEESLEAGLDFLI"
     gene            25890..26111
                     /locus_tag="VQ620_000988"
     CDS             25890..26111
                     /locus_tag="VQ620_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET2763634.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYYGTYGDIFFYLMKQDLSQYN"
     gene            26128..26442
                     /gene="trxA"
                     /locus_tag="VQ620_000989"
     CDS             26128..26442
                     /gene="trxA"
                     /locus_tag="VQ620_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET2763635.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
BASE COUNT         7412 a         5977 c         4942 g         8184 t
ORIGIN      
        1 aatcgtaaca ccacctttga acaagagtta cttcaaccca ttagctccga cggattaagt
       61 tgctttcgtg aataccaaaa tcagccgcaa tttcttcata agtgcggtat tctcgcacat
      121 atagaaagcg ttgttaattt atttatctca tttttcagaa aattctttta tttctgtaaa
      181 gtctacgata ctcgatgtgt ttttatataa tgatacagtc tgagaatcac tgttccgcta
      241 gccattccaa tagagattac caaagccaac atgacaacca aggtcgcact tgccagtgct
      301 ttattatagt cccctgtcac aaaaaaggca gttgttcggt aagagagata acctggaacc
      361 agcggtgcca aaatggccaa gataaagacc acagcaggtg tcttataaag aatacttaaa
      421 atctggctga cacaagaacc aataatggct gcaatgaagg tagctacaat gacattggtc
      481 ggttccttga gcaagagata gattagccag acagtcatgc ccaaaatccc tccaggtaag
      541 agcatagacc gttgcacatt gagtacgatt aaaaaagtga taatggcaag aaaacttgct
      601 actgcttgta ataaaaaggt tgttagtgtc atattagttc atcaatacca aggcgacaga
      661 agttcctgcc cctaaagcga gggtaatgag cagggattca aacatcttac tcataccaga
      721 gtttatgtgg ttggtcataa tatcacggac cgcattggtc aaggcaatac ctggtacaaa
      781 cggcatgacc gcaccagcta taatcaaatc tgccgttgaa ggaaaacctg tgtagcgagc
      841 ccaaaactgg gcaattatcc caaagacaaa ggctccagca aaggctgtca caaagggaat
      901 tcggataaat ttttccacat agagggaaaa ggcaaaacca aataaggtcg ccactcctgc
      961 cccaagtgcg tcgtagatat ttccgctaaa cataactgaa aagaaaggag cactaaaggt
     1021 cgcagccaga gttacctgca acttagtata gggaaggggt tgagcttgca aggccgtcaa
     1081 ttgcttaaag gctgtttcta agtcaatctg ccccccaact agctgacgag aaatctggtt
     1141 cacatcgcag actttttcga tgttataaga agaggaggtc acgcgcttca tgcgcgaaat
     1201 attggtattt tcaatagaga aaaagatagc ggcaggcatg gcaaggacat tgcaatccac
     1261 aatcccctgc gaatgcgcga ttcgaatcat ggtatcttct acacgatgga tttctgagcc
     1321 acttttaagg agaatagtcc ccgctagcat aatcacatca atgacggcat ttaattcttt
     1381 tgattcttcc atgctttcct ccttttatca actccctcta ttctatcaca aatctggact
     1441 caaaaaaaat ctttgccatg aaatcatgac aaagattgat tactcatttt gattatccat
     1501 ctgcttttaa ggagtagctg aagttgtttt aggtttgtag attgaaatct tgactctagt
     1561 cttatttagg tctacctttt cacctgctct aggactttgt tcaacaacca tgccttctac
     1621 actacctgca ggcgctgtcg tcacttctac aacttctata ttagcttcct taatcccaac
     1681 aatttgaatc aaattgttct tagtaaactc caagctggaa ccaatgtaac tcggcatggc
     1741 aacacttgta acttttttag ctactgtcaa gacaatttga gtaggtttac tcacatcata
     1801 agtcgttccg gcacctggac tttgtttcat aatcgttcct ggttcgcttt cgctggactc
     1861 ttcttcctct atcttaatca aattctcagg aaccttcttc tgcttgagtt ctgagattac
     1921 ttctgtagag ttccgtccaa tatagttccc taattgaatc gtcgtagctt ttttagctac
     1981 tgtcaaaaca atttgagttg ccttgctcaa gtcataggtc gtaccttctg gtagactttg
     2041 cttcaggacc gttccagcct cactttcatt cgactcttct tcctcaattt taatcaaatt
     2101 atctggaact tttttctctt ttaattccgc gataacatca gaagatttcc ggccgacata
     2161 attactaatt tggaaggatt gtttgcctga tgagacaacc agattaattt tcgttccttc
     2221 ttttcgacca gttccagcgc caggatctgt acggataatc cgcccttctt ccaccttttc
     2281 actagcctct gtcttctcct caccaatctc aaaattggct tttttgagcg ttgccttggc
     2341 ctctgcaact gtctgacctg ccacatctgg aatggcaatg gttgcaggag ttctggatag
     2401 tatccaaata agagaagctg ccaccaatac aaggctggcc aacaaaatca ggtaacgcat
     2461 cttaaatcta tgttttttcg gtgcttgtgg ttggtaagtt tcctctgtca cagcctggct
     2521 tgggtttttg attgatttgt gttctgtctg cgcttgaacc ttaggaatag atgtcaaggt
     2581 actctgagaa accttcggca aggtcttggt atctgccttg ctcgtttcat caaagattaa
     2641 cttactttca tttctacgat tgtaggacaa gctactagac aagtctacat acatctctga
     2701 aaccgagcga tagcgattgg tcaacttttt agcagttgcc ttgataataa cattttctaa
     2761 agcctgaggt acagatgaat tttctgcaat aacggacggc aggggtttct ggaaatgctg
     2821 gagggcaatg gtcaccgcgc tatccccgtc ataagggata tggcctgtca acatctcata
     2881 gaaaataatc cccatggcat agatatcact ctgcacagtc gccttcgaac cacgcgcctg
     2941 ctctggtgac aagtaatgaa ctgagcccaa catcgagtta gtctgggtca gacttgtctc
     3001 tgcaaaggct acagcaatcc caaagtctgt gaccttggca gtcccatctg gtgtcaaaag
     3061 gatattttga ggtttcaagt ccctgtgaac aattcctcga gtatgggcca agcgcatagc
     3121 caagagaatt tgtcccatga tacggactgc ttcttcatta gaaagaggat aatgttcctt
     3181 gatatagcgt ttgaggtcta gtccagcaac atactccatt gcaagatact gttgaccgtc
     3241 ttcctcacca atatctgtta tccgaacgat atgaggatgg tctagatctg ccatagctct
     3301 cgcttcacgc tgaaaacgag ctacagctat cgggtccgtc tggtagttgg tcctcagaac
     3361 cttcactgcc acttcttccc catctaagat taagtctttg gctaggtaga catccgccat
     3421 gcctcctcga ccaatctgtt tgacaatccg atagcgtccg gcaaaaatct tgccgatttg
     3481 gatcattctg catcctcctc gttcatagaa acaagggcaa ccgtaatgtt gtctaaacct
     3541 cctgcattgt tagcaaaacg aacaagtgtc tccgttttat ctgctaaagg aatatcactg
     3601 gttacaatat cacgaatctc actgcctgaa atcatgttgg tcaagccgtc actattgagc
     3661 aagagatagt cacctgactc aaggataact gtcccaaaat caggctgaat ttcatctttt
     3721 tgcccaatag actgggtgat aatatttttt tgcggatgag cttctgcctc ttctggtgtc
     3781 aattgaccag ccttgagcaa ttcattaacc aaggaatgat cgctcgtcaa ctgatggtat
     3841 tcttctccac gaatcaagcc gatacgcgaa tcaccaatat gagcatagat agcctgatta
     3901 tcaataatag caaggacttc caaagtagtt cccatgcctc tgtaagcttc atcctgacca
     3961 agctggtgaa tcttttgatt ttcaatttct aggtaatggg cgaaccattc acgcacttca
     4021 ttgactgtat cgatctgggt atcaacccaa gctacaccca ggtctgtcac cgccatttca
     4081 ctagcgatat tccctgcgcg atgacctccc atcccatcag ctaaaataat catggtacgt
     4141 ccagctctat tgacatagtg gttgacatag tcttggttat ttgttcgttt ctgaccaaca
     4201 tctgttaata atgaaatttc catgtgtcag ttccttccta atccgatatc ttgcgaaatt
     4261 gactgatgaa gaatccatca cttccataca attcaggtgt aatgaggata cagccgtctt
     4321 tcatgatatc cttacattca tgttctagtt ttacctgctc gaactcggga tgactctcta
     4381 aaaaggcctt aacgacttga aaattctcct ctgagacgat agtgcaggtg ctataagtta
     4441 ttataccacc tttgcctagt atttgacaaa cactacctaa tatttctaac tgaatttcct
     4501 gcaaggacgc gaaatctgcc gtttctttat tgtatttgat atctggtttt cggcgcaaaa
     4561 gaccgattcc tgaacaagga gcatccacca aaatcttatc aaaggaatcc tggtcaaaaa
     4621 actcatgcac ctttctggca tccaattttt gagtttgaac ccgatcttca actcccagac
     4681 gttgggcatt ttcttgaatt aaatccaact tgtggtcgta caagtccaga gcagtaacct
     4741 gacctgtcgt aagataagag gctatatggg ctgttttccc acctggagcc gcacaggcat
     4801 caagcactcg ctcatcacct tgtaaatcaa gcgtcggagc aaccagctga ctggactcat
     4861 cttggatggt aatggctcca tccgcaaaca aattatgccc tgcaaaatgc ccctgctcct
     4921 taaccagacc agtggttgct aaaagggaat tattcgcctc caacaaggct tggatttcct
     4981 cttttcgact taggtctgtt acacgaatac tggctttgtt tcgcactaac aggctttcaa
     5041 agatggcttt tgctctctcc tctccgtatt cttccttgag tttggcaact agccaaactg
     5101 ggagagaata ggcaatggag tcacgcttgt tttttcgctt gatgctagca atatctggcc
     5161 agccttcacg caagatacgg cgaaggacag cgttgaccaa tttttcactg ccttttttac
     5221 ggagtttggc caattccact gcttcattaa ccacagcatg atctggaatc ttgtccaaat
     5281 agcggagttg gtaggcactc atgagaagaa ggacatagag ccagctgtct aactggtctc
     5341 tgtcttcgat aaagtgggat aggtaccatt ccagagtcag tttacgggct accgttccat
     5401 agaccagctc ggtcactaag cccttgtctg ctgccaaaag ttgacttccc tttagatgct
     5461 tatttaaggc gatatttgaa tatgcttggt tcacaaaaac atcctctagc actgctagag
     5521 ctaaacttct agccgtttct actttagtca ccaaatcgtt ctcctacagt caatgtacgt
     5581 ccaactccgt tgaggaagga agcaatctcc atcttaggct taccagctgg ctgcacttgt
     5641 ttgagggata gagccccttc agccgttgcg acaatcaatt ctttcttgcc gatagagaga
     5701 atctcacctg gatttccctg accttctact ggtagggctt cataaatctt aaagcggtcg
     5761 cccttaagga aagtatgggc aacaggccag gggttcattc cacgaatttg gttaaagagt
     5821 tgacgattgg ttttgttcca gtccagtttt tcttcctctg gctttatatt tggagagaag
     5881 gtaacctgac tcgtatcctg cggttcaggt ttgatatcac cagcaatata ggcaggcaga
     5941 gtgtccaaaa gcaaatcacg accaactagc gccaattttt caaacaaggt gccaacattg
     6001 tcctcatctg tgatcggaat gctgcgacga gaaatcatat ctcctgcatc catttcctta
     6061 accatttcca tgatggtcac accagcttcc tcatcccctt gaatcaaggc ataatggata
     6121 ggcgcaccac cacggtgtct aggaaggagg gaggcatgaa cgttgacagc aaagtccatg
     6181 ctatcaagga gtttgcttgg gagaaactgc ccaaaagcag cagtcacaat tccatctgct
     6241 cctagcttca taagatcttc catctctgga cttccagata atttttcagg ttggtagata
     6301 gatagtcctg cttccttggc agcctgcttg actggggttt cttggataac ttttttacga
     6361 ccaacagcac ggtctggctg ggtcacaacg gctagaattt cgtaacggtc atctgtcaaa
     6421 agtcctttta agactgttgc tgaaaagtcg ggggtcccca taaagattag ttttgtcata
     6481 tcttctcctt cttataaaaa ttgctgcggc tcatggtcaa tactgagacg gagctcacta
     6541 ttttcccgtt cttgagtcaa ggccaagacc tggttgaggg tcggccccag ctcatcttct
     6601 aaacggtatt taattaaaat ctggtaatga tagaggttgt gggtacgggc aatcggtttt
     6661 ggcgttggcc ccagaatggg actggtctct gacaagcctg accgcaaaat gttcatgact
     6721 tcataggcac gtttgaaaac ctcttcttct ttcttgtgag aaagggtaat accaatcgtg
     6781 aaatagtaag gtggatagcc gagttgtcgt ctgattccca tttcataggc ataaaagcct
     6841 tcgtaatctt gatccttggc aaatcgaata gcatagtgct gcggattgta ggactgtatc
     6901 aagacttgac ctgccttttc agcccgacct gctcgacctg ccacctgagt caagagctgg
     6961 aaggttctct cagaagaacg gaaatcaggc agattcaagg ccgtatccgc atttagaact
     7021 ccgactaggg taacattggg aaaatccaaa ccctttgcaa tcatctgagt accaagtaaa
     7081 atatccgctt cccctcgccc aaactggtca agcaaggctt ggtgactgcc tttctttcga
     7141 gtcgtatcca catccatgcg cagaatgcga gcttggggaa agagttctgc tagctcatca
     7201 taagccttct gagttcccgt cccatagtaa cgaatactgc ggctcttaca gttaggacag
     7261 acctgaggaa tatccttcga gaaaccacaa taatggcagt tcatagtctt ggtatccata
     7321 tgcaaggtca gagaaatatc gcagttggga caaatatcca ccgtcccaca ctcccgacac
     7381 ataacaaagc tagaataacc acggcgattg agcatgagaa ccacctgctc ttttttaacc
     7441 agacggtctt ggatagcctc tagcaaagga ggcgtaaagt ttgacgtctc attttgtccg
     7501 atatagtctc gaaagtcaat cacttgaacc tcagggattg tagccaaagg attggcacgt
     7561 tgggttagac gtaagtgttg atagacgcct ttgccagcac gcgcccggct ctctaagctc
     7621 ggcgttgcag atccaagtac cagagttgct tgattatact gagcccgtaa aatagctacc
     7681 tctctggcat ggtaacgggg attgctgtcc tgcttataag ccgcttcatg ctcttcatca
     7741 ataatcatga tacccagatt tttcagagga gcaaagatag cagatctggc accaacaaca
     7801 acttgggcac cgccacgctc caccttgcgc cattcatcat acttttcacc attggataat
     7861 cctgagtgaa gaatggctac cttgtcccca aaacgtgcta taaaacgctc ggtcatctga
     7921 ggagtcaagg aaatctcagg taccagcaaa atagctgtct tgcccttatc cagggcacct
     7981 tggataatct gcaagtaaac ctcggtcttc ccacttcctg taatcccttg aagtagaaag
     8041 ggaggttgag aactgccaat agaactcaca accgcatcac gcacctgtct ttgttctgga
     8101 tttaactcca aaggtctact tgcttcaatt ccttcaaaat aagcagccga gcgttgaact
     8161 tccttttgga ctatggtaac agcaccttga tccacaaaga agttgacttg ctctcgcgag
     8221 taggactcta acaagctagc caaggaagcg ctctctggat gagacagcag ataatctctc
     8281 agttccaact ttttcttggc acgtgtagaa atctcaacac cttctaattg agcatggtca
     8341 acctcatacc aagactgggt cttgaccttc ttttgatcga ctgcctgata ttccagacca
     8401 agcaggcctt ttctagtcaa acgcatcatt tcagcttgct tggcaaggtc tagtgaagaa
     8461 aaggctagcg aatcttctga accaaacagg cgcactcgtt cttcctgact caagccttcc
     8521 agaggataga gaatcttgtc atagctagaa ttcagaaatc ctggaagcat ggccttgagg
     8581 atagagattt tgtaggagaa gacagatttg cgtaactcct cagccagcca gagttgttct
     8641 ggcgtgagaa caggagaaaa atccagcacc tctgcaatat cttttaaatc ttgctccatc
     8701 tcttctccat ctgattggga ctccaaacca agaacaatcc cttgaatcag gcgattcccc
     8761 ttaccaaaag gcacatgaac ccgcatccca acttccagca ttccctcaaa ttcctccgga
     8821 atcctgtaac tatagggctg gtccgtctgc atcaagggca catctacgat aatcttagct
     8881 agggccatct tctcacctcc tccttgtcag tacattcttg caatagaaaa aataagattg
     8941 agtcccccca accttaaatt ttttcaccat cttctttttc tttagcaatt tgctctttga
     9001 ttttcttttc ttcttcttct ttgcggcgtt tttcttcttc gatacggcga cgcactgctt
     9061 cacgttttcc ttctggttct gggtgaattg taacgtttcc tgattcgatt tcttctaaag
     9121 cgcgaagagt tgatttttca gacttaaaac cttgagttgc tggggcacct gcttccaatt
     9181 cgtgggcacg ttttgcttcc aagattacga gtgaatattt tgaaggaacc ttgtcgagca
     9241 aggtatcaat agagggtttt aacatcattt gcttgtacct attttctaaa ttttatcggg
     9301 tagttggaga ttttggtaac atctcctgat agtgaccaat gacacgatcc acacagaagt
     9361 gttctgcttc aatcacacat ttgacacgtt cagcagctag gggcacctga tcgttgacaa
     9421 tcgcataatc atactcacgc atgagggcaa tttcttcctt ggccttttcg attcgttggg
     9481 caatcacttc tgcactatct gttccacgac ctaccaagcg atcttgcaat tcatccaaat
     9541 ctggtggtgt caggaagata aagacagcat ctggaacctt tttcttgacc tgaagagcac
     9601 cctgaacttc aatttcaagg aaaacatcga ttcccttgtc caaggtttca ttgacatagg
     9661 tcagaggagt tccatagtag ttaccgacat attctgcgta ttccaacatc tgtccttgac
     9721 gaatcagctc ttcaaattct tcacgagtac ggaagaaata gtcaacaccg tccacttctc
     9781 caggacgttg tgcgcgtgtc gtcatcgata cagagtattg aaattggttt tcagaactct
     9841 caaaaatctc tcttctaacc gttccttttc caacccctga aggaccagaa aaaacgatta
     9901 gtaagcctcg gtctgccatt gtgtctcctt ttagtcaatc tgtgaaataa catttctcta
     9961 gaataatggc aaaaagccag attatccttt acagtctttc tatctagtgt aacaaaaaag
    10021 cagtaatttt tcaactgctc tttcttattt atttagcata atctactgca cgaagctcgc
    10081 gaatcacggt taccttgata tttcctggat aatcgagatt gttttcaatt ttcttacgaa
    10141 ctttgtgagc caagattgtg actttgtcgt ccttgatttt tcctggattg accatgatac
    10201 gaatttcacg tcctgcttga agggcaaagc tagtttgcac tccttcaaag ccgttagcaa
    10261 tttcttccaa atcatggaga cgcttgatgt agctttcaag agactcacta cgagcacctg
    10321 gacgggctgc gctcaaggca tctgctgcag cgacgataac tgctatcacg ctctcagctt
    10381 caacatctcc gtggtgacta gcaatcgcat tcaccacaac tgggggttcc ttgtacttac
    10441 gggccaattc cataccgatt tcaacgtggc taccttcaac ctcatggtca atggctttcc
    10501 cgatatcgtg aaggaatcca gcacgacggg caagagccgc attttcacca agttcgctcg
    10561 ccatgatacc agccaactta gcaacctcaa tcgaatggcg caaaacattt tgtccatatg
    10621 aagtacggaa ctgcaaacgt cccataatct tcatcaagtc tggatgaagg tttggcgcac
    10681 caatttcata ggcagcagcc tcaccgtatt cacgaatctt attgtcaatc tcttgacggt
    10741 ttttctcaac caactcttcg atacgagctg gatgtatacg accatctttg agcaacattt
    10801 ccatagtcat acgggcaatc tcacgacgaa tcggatcaaa tcctgacaag gtcaccactt
    10861 ctggtgtatc gtcgataatc acatcgaccc ctgtcaaact ttcaaaggta cgaatgttac
    10921 gaccttcacg accaataatg cgtcccttca tagtatcgtc tggcagatga actgttgagt
    10981 ttgttgactc cgctacatat tcaccagcga tacgttgcat agcttgaacc aagatgtcct
    11041 tggccatttt gtcagaacgt tccttgacct cttgctcagc ttcgcgaatg cgactggcaa
    11101 tctccctggt caagttttcc tctgtctgag ccaagataat atctcgtgct tctgcctgag
    11161 acagagcacc aatacactct agttctgctt ctttttgtct ttcgacttcc tctaattgct
    11221 cttcacgcgc atcaaggttt ttcgctctat cagaaatact ttgttctttt tgttcaagtg
    11281 tttgttcttt actcgtcaaa ttgtcgtcct tacggtcaag gctagtagct ctctctgtca
    11341 aacgactttc gatttgtttg agttcttgac gttctgattt aaattcagcg tccacttctt
    11401 cacggtattt tctggcttct tctttggcct ccaatagtgc ttctttttta agagacttgc
    11461 tttcacgttt ggcttcatta acaagtaaat ccgcttcacg ctcagcttgt ccacgtaaat
    11521 tagttgcttc ttgttcagca tttaaaagca tcaactccgc agcttcctga gatgatttca
    11581 tcttagctga gatgctgaca tatccaatga ctaaaccaat gatgacggca aaaacagcaa
    11641 tcgcaagcga catgatctcc atgtttttac ctcattttat tgttattccg aatgacatac
    11701 attcttttac attctaccat aaaaaagtga ttttcacaaa cctaaaatag aatatgtttt
    11761 ggggaattta gtctgaattt taggattaca cttccttggt tttttaagga gataagtcag
    11821 tgtcgctatc aaaaagttcc accgaatttc tacaactatg gattttctat tgttgtgcca
    11881 aatagtgttc cactagattc aaacattttg ctttcaagtc tttgaaaatt cactttagga
    11941 aattacagct tatcttcaag atcaaatttg tcataaatta acttttttta tttagttgta
    12001 tcaatttatt tcttaagtat taatttatcc tcattgaaag cttttaaaat atactcatta
    12061 atcaatttat tatcttcttc attttttgaa aaataagttt taatattatt gggagttcta
    12121 tgttctagta gacgatggat aacatcatca cttctatcat ggaaccaggt ccaatctact
    12181 tcaggataaa gtcctcgaat atcttcttca atatctttta aaatctcaaa atcagaaaaa
    12241 tatccttttc tacttaagtt gtataaatct gatacagact tttctcttgg gtctgggaaa
    12301 gttttaaccc cctcaactct tttatcatta attcttttaa tcaaataatg gtagatttta
    12361 ttttgtatct gaggaaaatc atagacatta taactcatca aatcaattac tttatggtaa
    12421 ataatatttt ctgattcttc ataaactttt agagtatcta gaatgtcatc tttaatatcc
    12481 ttttcgaaat acttaaataa gtggcatatc aagtcagaat attctatgat attttttata
    12541 tcatcatttt tatattttat tcttaaaata tctgtattaa atttttcgag tactattatt
    12601 gcagtttcct gatcagttga gcagtattct agtaactgtg aatagagtgg atacactgaa
    12661 ttatataaag aaaaattttt ctccaaaatt tcatttaaat gtttatttac aatcccaatt
    12721 atcttagatt tcaatgaatc tttatcaata acatccatat tatcgagtac aattttaata
    12781 attctcctaa attcctcgct ccaattataa tacagtgttt gattatttaa aatttctatt
    12841 actttttcta tgtcaatatc atcaacaaga ggaagaaaaa ttagcatatt tttaagctct
    12901 cgaagatttt catcttcagt aatttcaagt ctttcttgaa gataagtaat tcgattcaac
    12961 aagtagtctt tcgcttcctc agtaactttt attttaccaa atgaatagtt tctaaaatat
    13021 aaatttacaa ccttaaaatc tatattcgga agaattaatt gaacatcatc caaatcaaga
    13081 tttttaagcc aagaagatgt tctatcgaaa atacttgagt caggatttac atatgaatta
    13141 tcataactga gtaataatat ttctaaatat ctattaatga ttgatttata aatcttataa
    13201 tgttcaacac ataaacaatt taattttaaa aagttataaa gattttttac tgtaaactcc
    13261 gcattaaata gatgattatt agcagaccat cctcctcttc tatagctttc atgctcttca
    13321 cgaatttcat caaataattc attaatagtt tctaattttc tatataaaaa attgaagtta
    13381 aggattgaat ctctaaaaaa ttctataatc tttttctcat ctcctgtaac cgagttaata
    13441 atttgttctg ttaattcgct taattctcca tcataaagtg gctctgcata actaggtgtt
    13501 ttttcgtatc tatcaattat ttgaatttgc tgaaaattaa attcacaaac taagaaatta
    13561 aaatagtctt tctgacgaaa tgctatatta gctagttctc taaattttgc ttttgctaaa
    13621 gaatattgtt tttcttcata caacttaaaa gccttactta agaatcgatt cccctttaaa
    13681 tccctaggct caccaatctc catatctaga aagcgattta ataaagattt tttagatacc
    13741 ataattcgta attcttcatg accatcaatt tcaaatctta attgatcatt tccaaaccag
    13801 caatactcat catcccagtt taaagccctt ttctttaaat cagaatatct agaaaaatct
    13861 ttcgcatcaa taaaactaag tctatctaaa aaagccagtt gattccataa atcatcagca
    13921 ttatttacat cttgacttcg gttttctgat agaacctcta aaaaatcttt tgtcctgcaa
    13981 tacaatttgt tctgtttttc tgatgtctcc tgacctagat tctcttcatt agagataatg
    14041 aaaattcctt gcttcttata atactctcga atgatcatag acggttcctt gggagtataa
    14101 aaataagcat ttttagcatc caatttaaat gtattttgaa tcattctaaa aattgaatta
    14161 aaagtcgaat cacttagaga ataccccaca aacaatagag tatgattcat tatcaatgac
    14221 tggattaaag tagaaatcat tgggaagttc aaatgatagt ctaaataatc atcttctttc
    14281 aaaacaatat ttttcttact gaagtcacca tgcattttaa tcaagtatct ttccgaactc
    14341 gtataaggga tgtctttatc ctcagcgact acattgtact ttaacaagcc actttcaaat
    14401 tgactctcca gaagtgaatc atagtttgtc gtaataatat gttttggtgc aattttttct
    14461 attaatttat gtaactcatt tggtttactc aatcccttct taaagaaaat ttcttctatc
    14521 ttctttgtgt actgattttt cccaaaagta tcataataat actgagcaat tctcagtgga
    14581 ctatctgttc tttcttctgg tatgttcaat tcttttttta atgattgaat caattctccc
    14641 caagttggta gattagaatt agctgatatt cctgcaccaa caaacaaaac taaggaattt
    14701 tctttaatag aattctttat ttgagttata gataaattat aattttcttc catattttaa
    14761 ctatcctatc attagcttgt attacataaa tacaaattat tcttataaat cttctttttt
    14821 attacataat cttaaacaaa agtttatcat ggtttataaa tataaaaatt tatttgtctt
    14881 ttatattcaa tttatgattt ttaatataac cagttctctt cattatacca caaaataaca
    14941 agcataaagc ggattcaaag ataaagatta aggttcatat actaattttc tttattgact
    15001 ttttgtcttc tgtattaggg agtttatcat tttccaaaaa ctttatcttc aatttgttag
    15061 ctaaaatttc tacaataaaa aagcccacct ttcagtagac ttagtttctt tctattctta
    15121 tcggcactct tccaaaattc tgttgtgcta tacttggatt tcctagttgg taaatctggt
    15181 cagccttttg agtcatagca tcccaaggtt ctttgccaat tcggctgact agactgacct
    15241 gacctttcaa agacttgaga tgttgcctgc ctttttcagt aaatccaagg acatgaatgg
    15301 cttctggcaa gacattttct ctagcctgca ccaaaatata ggtcaatagg cgtctgacac
    15361 gcgccttggt gtaacgtttg gtagtcacta actcgaccaa ctcttctaca gactgggctg
    15421 ttttaatagc ttctttaatg cgcacaacca tttcttgatt gacctgatag atggtggtta
    15481 ggtcgggatt tgacaagatt tgatagcgga gcaagggaaa atagtcttcc cagatcacct
    15541 tactggcctg ctcaaatagg gcaacagaag gcataaaacg ttctaagaaa tcttggtcct
    15601 tctgatgttg acggagggct gtcgccgagg caaagtccac atctttattc acagaatggt
    15661 aacctgcccc ctgacgctga atcggatgca gcttgatatt tcgtcctgca accgccttgg
    15721 cataggccag agcaagaaca tgattggggg tattacctga aaaatcaaga ccagcaaatt
    15781 ccttccacat agcttgggtt ttctggggat aggaaaggga atcaggcagt tttttcacaa
    15841 atttctccat ctcagcacct ttctctgtgt ataagtcagc gattttctgg taatccagaa
    15901 cttcctctgt cccaaaaact agactatcaa tgcccaagcg atccaagata tccacagctc
    15961 cttgaccaaa gaaatcagct gcctgaacac tgactaaaaa gggcaattcc actaccaagt
    16021 ctgcgccatt ttccagcgcc atctgggccc ttgtccactt atccacaata gcaggctctc
    16081 cacgctgcat gaaattccct gacatggcaa caattttcag tccctctgcc tgatccagca
    16141 ggtatttatg gccattatga aaaggattga actccgcgat aatacctgtt atggtcatgc
    16201 taatctccta cttctgcgcc acaaaaaacc aacgggtgct agtttctgtt ggctccttat
    16261 cctcaaagtc tgcatagagt ttgaaggact tgaatccagc ctgttccagc aagatatcat
    16321 aggtcaagat ctcataggtc ctctcctcat gtacttcatc gtggcgacta aaggaaccgt
    16381 cagcctcctt gataaagaaa gtcagctcat gcacgatgga atgaggagct tcgtcctcat
    16441 atgtatccca aagcatggca aaatcttccg cattttcatg gtaggaatag ccagggaata
    16501 cttcatctgt ctggtaggtc gagtgcacgt caaagatgaa aactccctct tcattcagca
    16561 cattgtacac gtccttaaag acgtccccta cttccacctc atcctgcata taacagatag
    16621 agtccgaata acacgtgaca aaatcgtatt tccctgcctt ggacaaattc agcatattac
    16681 cttctataaa agcaatcttt tgcttggctg aagttgctct cttctccgca atcttcaaca
    16741 tatccgcact caagtcaagt ccagtcacat caaaaccagc ctgagagaag cgcactgatt
    16801 gaattcctgt cccacaagcc aattccaaga gtttctttct ctccttggtc ttaggcaaat
    16861 gacgcagaga aaaattcgtc catttgtcgt ataaactatc gtccatgacc gcatcataga
    16921 ccgccgcaaa ggtttcataa gtcgccataa tcatgatacc aagagctcca tggtaaacac
    16981 cactagccct ttacctttct atcaattttt ctaaaataag caaagaaacc cagtcgacta
    17041 caactgagtt catcttctaa gcaagagctt ctgaaatatc tactgaattc gcctcatgcc
    17101 atagcttctc tagattataa tgggcacgca tttcttctga aaagatatgc acgacaacag
    17161 caccgaggtc cagtaagacc cagcctccag ctgcatcgcc ttcgacatgg ctacctttaa
    17221 agcctgcttg agctactttt tcacggatat tagctgcgat agcgtccaac tgacggctat
    17281 tcattgagct agtgatgaca aagtagtccg tcacactagt caaatcttgt acatcaagtg
    17341 cgaggatatc ctccgcacgt ttctcatcag ccgctttcac gactagttct agtaattctt
    17401 tttcgttcat ttaatcctct ttttagaaaa tatgtttgta atctaggaca tctgcaaagc
    17461 gctcctccca aatgtcctca taaaattcat taatcgtttc tgctggaatg tcatccccat
    17521 caaaggttgt gacagcacct tctggaacaa taagctgata gccatattca aaggcaacct
    17581 tgacagaggt atccacacaa tattctgtct gcataccaca taaaactaat ttttcaatcc
    17641 cctgcttatc caagtattct tttaagccag tttctttgaa aatactgtta tatttcttat
    17701 gaaagacctt ttcagcaggt tttcgattta aaagcgcaga taactgccaa tcttctgatg
    17761 tttgagcttc agagttctca atatgttgaa catagataat ttcaatattc ttgcttctag
    17821 cctggttttg taaataagaa attttttcca atagactttt tgtctggaac ccagtttcta
    17881 ctaaaacatt ctgaacatca atgattataa aagccatctt catttagttc tctttcaaat
    17941 agtgcacaaa ggcgttatag gtttcaagga tttggggata gatggggaat ccctgatgag
    18001 ctagatactc cacggtacga gctgtttcgt aggccaccgc cttattgagc gatagacttg
    18061 caatctcacg cgccacatct actccaggaa aggctcgatt gtgctcgata tagtctgcga
    18121 cgtagatgac cttatctagg tctgtcattt gaccagcacc gactgtatgg acttcaatgg
    18181 ctcgcaggat ttctgaatca tgcaaatcca aatcttcctg aattttgtaa attccgacca
    18241 taccatgcca gacattatta ccccagtttt tgaggtcgaa atctagctga taacggtcaa
    18301 tcaagtctag aaattcctga tctgacaact ttttagcata gtcatgaaga agacctgcta
    18361 gacttgcttt ctcaacatct acaccaaagc gctgagctaa ttccatagcg gcacgctcca
    18421 cacctagaca atgggttaga cgtttctcag gtagaagctc tgccattttt tccaacaaag
    18481 cctcacggga gcagttgata tagtcttgat aggacatcag tagagcccct ccttctcgat
    18541 gtagtctagc actggctgag gtaggagaaa gttgggtttc cgaccttggg caaggaaggc
    18601 acgcaccatg ctggacgaga tatccatgag cggtacgtcc acccagataa ctggatagga
    18661 agtccctgcc ttgtagcgtg gacgctgaac ccccacaaac tgaaccatgt caaccagttc
    18721 atcaattcgg taccacttag gcagatagtc aaccatgtcg gcgccgatga taaagtaata
    18781 atccgtatct ggatgttgct ctgtcaagat tttcatggtg tcgtaggtgt aggaaatacc
    18841 cttgcgctcc aactcaatgg tttcaatgac taggccgtca atcccctcaa ttgccaactc
    18901 aagcatcttg agacgatggt gttcaggtat ggtttccttt ttatcaacgt gaggaggttg
    18961 gtattcaggc atgagcagaa cttgatccag tcccaactgt tgccgtactt gatccgcaac
    19021 aatgagatgg gcattgtgaa cagggttaaa attcccccct aaaatcccaa cttgtttgcg
    19081 ttttttctcc ttgatttctg gctccaactc taccttggta aagggagtca atagttcgat
    19141 tgtcataggc tagtcccctt tatttctcta taaaaacagt tatttggagt ttcggtctta
    19201 gatttctttg actttcttag aaatcttgcg attttctttc ttgctagatt gtttaaacaa
    19261 aatcaagatg cgtcctattt tttggactgt atccacaccg atttcttctt ccaaaatttc
    19321 agctacttcg tggatgtttt catctgtgtt ttgtaagaga gtaaccttga ttaattcacg
    19381 cgcatcaaga gcttgacgga cgctggtttt gatttggtcg ttgagtccat ttttcccgat
    19441 ttggatgata ggtttgaggg tgtgtgcctg gctgttgagg aaggcacgtt gttttgatgt
    19501 taatgacata attttcttcc ttgttttttg atagttattt taggtggtga gggacggtcg
    19561 gaactaattt tccaaagatt tcatctttga aaaatggatt tcctgacctc acaattgagc
    19621 cttggtctca attgtgcccc ttgccaatct acagattggc aatcacacca cggcaataac
    19681 tatcgctata tgagctcact ttgttcgctc agcgattttg ttttaaataa ttgcttttcg
    19741 tgtgacgacg gcgacgcctt ctggtgccca gacggcgact tttgctgtgc ctgttacacg
    19801 aatccagcct aggcctgaga tgactaggtc tgtcttatct ttaatggtaa agacatgctg
    19861 gactagcctt gggaaatctt ctttttcctt gctatttggt ggtgtcagaa gagttcccag
    19921 gtgcttatcg tagaaagcac tagctccttc aagcttgcta cgatggagtt tgagttcatt
    19981 atcaaagaaa gcagtaaatc cttgcttttc tcctgctata aagtcaaagc gtcccaaacc
    20041 acctaaaaat agggtttgct caggattaag ctgataggtc ttaggcttga tttccttttt
    20101 aggactgaca tacttgaggt ttttggccgt caagtagtga gccatctggt ggcggtggat
    20161 aattcccggc gtatcgtaaa tataagatcc gtcgtcaagc ggaatctcta ttttgtccaa
    20221 ggttgtccct gggaagcgtg aagtagtgat gacattctga tcacccgtga tttcttggat
    20281 aatagcattg attagagttg attttccaac gttggtcaca ccgaccacat agacatcgcg
    20341 gcccttacgg tagtgttcaa tcttgtcaat gacttcctta atggcatgtt tattttgtgc
    20401 tgaagttagg accacatcga ctggacgaag accttcttca tgggcacgtt tcatgagcca
    20461 ctggctaatc ttaccagact taactgactt aggaaggata tcttttttat ttcctaccaa
    20521 gaggacatca ttgcccgaga cgaaacgtgg taaacctggg atgacagatc cattaaaatc
    20581 aaagatatca atgacattga ccactaaggc attactgtct cccacctcgt gcaagagctt
    20641 gaggaaatca tcgttcgtca actggacatc tgtgatttca ttgtagtggc ggagacggaa
    20701 acagcgttgg caatagactt cgccagtctc caaacctttt tcaagtgccg actggggggt
    20761 aaaaccaaga ccagctttat ctgtcgtctg aatggttgct ccacaaccaa tacagagaat
    20821 ttcttccata gttaaattcc ttttttatat gtaatcggtc cgtacttttc agtgattttt
    20881 ctcataacac gacgctcacg agttcggtta atctgcgttt tgattgagtc atgttggacc
    20941 aagggtttga ctaaaattga ccgaatccct gcacggtggg ctgctcgtat atctgtcatg
    21001 agctggtcac caaccatgac cacttccttt ttgtcatagt ggaattcctt catagcacgg
    21061 tcaataccaa atgtgaaggg cttcaaggcc cagtaaacgt aatcaatccc aaatttctca
    21121 actgctcgtt gaacgcgttt tttggtgtta tttgacacta cgataatgcc aatacccgcg
    21181 tcccgaaggt catgtagcca ttgcttcatc tctggcgttc catcagggtt gttccaagca
    21241 atgagggtat tatccaaatc gaccaaaaca gccttgattc cctgcgcctg caggcttggg
    21301 actgtcagat catagactgc ttccacagca aaatctggta tataattttc aatcgccatt
    21361 ctggctcctt ttcttaactt tttttcgcaa ttttccttag taacaaggat aaaacaatcc
    21421 acaaacctgc actagccaaa ctgatataga gccaagcatg gggctcatct gttaaaggta
    21481 agggaacatt cattccgaaa aagcctgtca cgactgccaa aacagctagc aagactgaaa
    21541 tgatagtcaa ggttgtcaaa ttgtcattca gattattgtt tagaatattg ttgtaagagg
    21601 ctgaaagctg ctgtaaaatc tgagagatta ggtctgtcat ggataccagc tgatgagcct
    21661 caatcatggc atcatcaaac tgttctctct caatctcatc aaaactacga tacaaggcat
    21721 gaccttgaat atgctctaac aaaatccgat tttgtttggc agctgccgtt agataaacca
    21781 taccagtctc caaatcagac aaggcaaaga ggtttttctt ggtagttcgc tggcgcaaga
    21841 ggtcattgac ctcatcccta ctcttgtcca tctgctcaat gacaggatag taggcattgc
    21901 tgataatttc cagactggca aagagaaact tataaatcga aagcgtgtca tggctctcca
    21961 gataacgagt catctgttca atgacatagg cgttcttggt attactaatg gtaatcaggc
    22021 gacgatgctc gacgataaag gtcatgggaa aagtctcata gtaggccttg tcctttttta
    22081 cgtctaggac attatagata aaggtaaccg tcccactttc acggtggtag tccatgtggg
    22141 cgcgctcgtt tctatccagt gcgtattcaa tggtttcctt gtccaaaccg taaatttcag
    22201 aaaggtcttc tagtttattc aactttccta ggtctaggtc tatccaggta caaccattgc
    22261 ccaactgctt ttctaaaacc atcttttctc cttattaaac tccctctatt gtaccacaaa
    22321 ttacttaaaa taacaggtct agtctgtgcg tgagaaattg ctaacaactg tgatatttcg
    22381 gttagggaca aagtgaaggg cttgctcctc gctacgaagc tgtgtcgtac gaattcccac
    22441 actgacaacc ttacccgata cagtaatcgg tccatttgtc agaacgacct catctcccac
    22501 atccagttga cgttcaaaga ggatgaaaaa gccattgatg acatcagaca gaaagccttg
    22561 ggctcccata ccaatcgcta ccccagcaat accagctcca gccagcaaac tagaaactgg
    22621 caaacctaaa atcgacaaaa tgcagtagag taaaaagaaa tatagcgtat aattaaacac
    22681 attttctagt aaacgtgaga tggttttttg gcgtccaaca tcatgacgag acatttttag
    22741 agaaggtttg acaattctct gcaccatggt atgaagcatt tttttagcaa tataaaatac
    22801 aatcaaaagc agtaaaagag aaatgacctt ggttagaata ttctcgataa tggttgtcac
    22861 atctagcttt tcaatataag cctgaataaa tttttgcata taaaactcct ttatttcatt
    22921 ataccatatt ttcatcagtc tcattctcca taaatattca aaaaatagac agaaaattct
    22981 tgattttgtc tatcatttat actataatca taatcactac acaaaaagga gattattatg
    23041 aaaagaatca ttcatgcttg gaataaggca agccttgtca agcgtatctt gattggtatg
    23101 ctttgtgggg aatcctagga ctgacccttc ctaatctctc agggattggg ctactcgagg
    23161 atttattcgt tggaggtcta aaagctgttg ctcctatcct agtctttgct ctcgttgcca
    23221 atgccctttc ccaacatcaa aagggacaag atagcaatat gaaaactgtt gttttcttgt
    23281 atatcctatg atagggactt tcgccgctgc tcttgtagct gtactagcaa atttcatcgt
    23341 ccctattgaa attaccccaa atagtgccaa tactgaaatt gcaccaccag atgggattgg
    23401 gcaggttctc agcaacctct tgctcaaact ggttgacaac ccagtcaacg ccctgcttac
    23461 tgctaactat attagaatct tatcttgggc agtcattttt ggaatcgcta tgagagaagc
    23521 cagtaaaaat agtaaagaat tgctaaaaac tatcgctgac gtgacttcta aaattgtcga
    23581 atggatcatc aatctggctc catttggaat ccttggtctt gtttttaaaa ccatttctga
    23641 caagggagtc ggaagccttg ccaactacgg tattttattg gttctattag taacgactat
    23701 gctttttgtt gcccctgtgg tcaacccttt gattgccttc ttctttatga gacgcaatcc
    23761 ttaccctcta gtttggaact gcctccgtgt cagcggtgtg acagcctttt tcactcgtag
    23821 ttctgcgact aacattcctg tcaacatgaa actctgccat gaccttggac tcaacccaga
    23881 tacctattct gtttctatcc cactcggttc tactatcaat atggctggag tagcgattac
    23941 cattaacctt ttgacccttg ttacagttaa cactcttgga attcctgttg actttgccac
    24001 agcctttgtc ctcagtgtgg tagcagctat ctcagcctgt gatgcttcag gtattgccgg
    24061 aggttccctc cttcttatcc cagttgcttg tagccttttc ggtatttcta acgatattgc
    24121 catacaagtt gttggggttg gttttgtgat tggtgtcatc caagactcat gtgaaacagc
    24181 ccttaactct tctacagatg tcctctttac cgccgttgcc gaatacgcag caacccgtaa
    24241 aaaataactc atcaaggcaa gcctgctcat gtcttgtctt ttacgctttt attctaactt
    24301 attaggaaat tcttatgtct attagccaac gtacgaccaa gctcatctta gctacctgtc
    24361 ttgcctgcct gcttgcttat tttctcaatc tttcgtcagc agtttcggct ggaattatcg
    24421 ctctcttgag cctatctgat acgcgtagaa gtactttaaa actggctcgc aatcgtcttt
    24481 tttctatgct tctagctctg gctatcggtg ttctagcttt tcacttgagc ggatttcata
    24541 tctggagtct cggcctctat ctggccttct acgttccttt agcctacaag atgggctggg
    24601 aaattggcat cacaccaagc actgttttgg ttagccatct cttggttcaa gagtcaacct
    24661 ctccagacct tctagtcaat gaattccttc tctttgctat tggtacagga tttgccttgc
    24721 ttgttaatct ctatatgcct tcacgagaag aggaaattca gcactaccac acgctggtgg
    24781 aagaaaagtt aaaagatatc ctccagcgct tcaaatacta tttatccaga ggagacggac
    24841 gcaaccgagc acagctggta gcagaattag acacgctttt gaaagaagcc ctcagactgg
    24901 tctatttgga tcactctgac cacctctttc accagaccga ctaccatatc cactactttg
    24961 agatgagaca gcgacaaagt cgtatcctga gaaacatggc ccaacagatt aacacttgtc
    25021 accttgccgc cagtgaaagc ctgatcttag cgcaactctt ttcaaaaatt gcaggtcaac
    25081 tgagccagac caatcctgct tctgatttgc tagatgaaat tgaacgttat ctggaagtct
    25141 tccggaaccg cagtctgccc aagacaagag aagaatttga aacccgcgcc acccttcttc
    25201 aactcctacg tgaagccaaa accttcatcc aagtaaaagt tgatttttac caaaaatata
    25261 gacagtaaaa aataaaacca ctaaaaaaca gagcttgcca gcattcttga aaagtagtat
    25321 aattattgca tgcgcaatca tcttgtgacg cagagactgc ccgttaatgg actttcttct
    25381 atttatgact ctaaaaagaa aggagacact atgacctatt tggaaaaatg gtttgacttc
    25441 aatcggcgtc agaaagaaat tgaaagtctc ttggaaaaga ccattgccca acagagcgag
    25501 caaagtctga ccttgaaaga gttctacctg ctctactatc tggacttggc tgaagaaaaa
    25561 tctctacgcc agattgacct gccagataag cttcatctga gcccgagcgc agtttctagg
    25621 atggtggctc gcttggaagc taaaaattgc ggtctactca gtcgcatgtg ttgccatcaa
    25681 gatagacgct ctagttttat ctgcctgaca aatgatgggc aaaagatact ggcctcgcta
    25741 caaaagactg tcgaagaaag cttagaagca ggtttggatt tcttgattta agatgatttt
    25801 aaaaaaagtt gcgtgcgcaa tcatttttct tgacattctc ttttataagg agtaaaataa
    25861 agttagcatt aaacaaagga gttttaaata tgattgaaat tacctatata gatgccagca
    25921 agaacgaaag aactgtgact ttcgagtctt atgaagactt tgaacgttcg caacaagctt
    25981 gccttatcgg cgtcgcagac tactaccctg tccaaaaatt aacttacaag ggtcataatt
    26041 tggactacta tgggacttat ggagatatct tcttctatct catgaaacaa gatttaagcc
    26101 aatataacta aaaaaaggag aaatacaatg gcaaaagcaa ttacagatgc aacattcgaa
    26161 caagaaacaa aagacggttt ggtcttagta gacttctggg caacttggtg tggtccatgt
    26221 cgtatgcaag gtccaatctt ggacaaattg tctgaagaac tttcagaaga tgtcttgaaa
    26281 atcgttaaaa tggacgttga tgaaaatcca aacacagctc gtgcttttgg aatcatgtct
    26341 attccaactc ttctcttcaa aaaagacggc caagttgtca aacaagttgc aggtgttcac
    26401 acagcagaac aaatcaaggc catcattgct gaattgagct aatcaaacga gagaccaagt
    26461 actttctttg gtctcttttt tcttgccctt tgccattttt caaaaaatat gctag
//
LOCUS       DAVBGY010000022        26390 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVBGY010000022 DAVBGY010000000
VERSION     DAVBGY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26390
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.022"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            140..516
                     /gene="tnpA"
                     /locus_tag="VQ620_000990"
                     /pseudo
     CDS             140..516
                     /gene="tnpA"
                     /locus_tag="VQ620_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775157.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(904..1533)
                     /locus_tag="VQ620_000991"
     CDS             complement(904..1533)
                     /locus_tag="VQ620_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET2763636.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(1543..2544)
                     /locus_tag="VQ620_000992"
     CDS             complement(1543..2544)
                     /locus_tag="VQ620_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET2763637.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSLICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(2575..3216)
                     /locus_tag="VQ620_000993"
     CDS             complement(2575..3216)
                     /locus_tag="VQ620_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HET2763638.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(3235..4050)
                     /locus_tag="VQ620_000994"
     CDS             complement(3235..4050)
                     /locus_tag="VQ620_000994"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HET2763639.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKKIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASSFVNGHILYVDGGILAYIGKQPE"
     gene            4321..4755
                     /locus_tag="VQ620_000995"
     CDS             4321..4755
                     /locus_tag="VQ620_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2763640.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNILSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            4767..5957
                     /locus_tag="VQ620_000996"
     CDS             4767..5957
                     /locus_tag="VQ620_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HET2763641.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELAHHDLGFLYTPSCMAEYKINGDGEAREAALKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEYYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            5968..6459
                     /locus_tag="VQ620_000997"
     CDS             5968..6459
                     /locus_tag="VQ620_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET2763642.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            6474..7253
                     /locus_tag="VQ620_000998"
     CDS             6474..7253
                     /locus_tag="VQ620_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2763643.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            7240..8058
                     /locus_tag="VQ620_000999"
     CDS             7240..8058
                     /locus_tag="VQ620_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2763644.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            8058..8351
                     /locus_tag="VQ620_001000"
     CDS             8058..8351
                     /locus_tag="VQ620_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET2763645.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            8373..10274
                     /locus_tag="VQ620_001001"
     CDS             8373..10274
                     /locus_tag="VQ620_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET2763646.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            10334..11335
                     /locus_tag="VQ620_001002"
     CDS             10334..11335
                     /locus_tag="VQ620_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2763647.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKKLNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(11445..11780)
                     /locus_tag="VQ620_001003"
     CDS             complement(11445..11780)
                     /locus_tag="VQ620_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763648.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTTPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(11791..12033)
                     /locus_tag="VQ620_001004"
     CDS             complement(11791..12033)
                     /locus_tag="VQ620_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763649.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(12044..12586)
                     /locus_tag="VQ620_001005"
     CDS             complement(12044..12586)
                     /locus_tag="VQ620_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HET2763650.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIILFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSVLGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            complement(12602..12796)
                     /locus_tag="VQ620_001006"
     CDS             complement(12602..12796)
                     /locus_tag="VQ620_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2763651.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            12962..13912
                     /gene="rsmH"
                     /locus_tag="VQ620_001007"
     CDS             12962..13912
                     /gene="rsmH"
                     /locus_tag="VQ620_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET2763652.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIHQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            13924..14241
                     /gene="ftsL"
                     /locus_tag="VQ620_001008"
     CDS             13924..14241
                     /gene="ftsL"
                     /locus_tag="VQ620_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET2763653.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            14245..16497
                     /gene="pbpX"
                     /locus_tag="VQ620_001009"
     CDS             14245..16497
                     /gene="pbpX"
                     /locus_tag="VQ620_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HET2763654.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKATAETFAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            16499..17479
                     /locus_tag="VQ620_001010"
     CDS             16499..17479
                     /locus_tag="VQ620_001010"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET2763655.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGIIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            18088..20193
                     /locus_tag="VQ620_001011"
     CDS             18088..20193
                     /locus_tag="VQ620_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2763656.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTSEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSQLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMKDGVLVIREKA"
     gene            20915..21934
                     /locus_tag="VQ620_001012"
     CDS             20915..21934
                     /locus_tag="VQ620_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HET2763657.1"
                     /translation="MSNTLKQLLLFREEFIKNILNGIDVDETVVIENDFVEELENFLK
                     ENDLLDFSNSIFFQLSGDKVIVNNIYPGYLAYFNRFLTFYPKIFAIKEVGEYIDFLNF
                     EKRIADMFYCWGFNANSRVLTAEKIIEIPNHQINAKYIKKVIKMSELLFGVDSNNRIR
                     LLNNRRELIKTIFQGSVIKSAIPALAATLDTISNSGSFQISLSKQIINNLVLSGKDKV
                     TIPRIELDNVVLSRQKIIVSVSYINDFLDLRFPDKIEYILNFLEGSGFNTKVMVYFGK
                     IVEEDKNYFVNLEKPQYFDFTSILFNKLFFKELNKFDYMVIEELIPNLDEDVMMTEYI
                     KEYTE"
     gene            21947..23050
                     /locus_tag="VQ620_001013"
     CDS             21947..23050
                     /locus_tag="VQ620_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763658.1"
                     /translation="MILSKVRCYIYNSKIRGKVLYELYEFINENKQLMDAINSIKVDS
                     SNGFSIQILHLGEQQTTVLLTDFFTKTLDLFKEFRVTNKSTLEQVKNQISILNSSGPK
                     MDLTNIKTDYSIVEEKLDWQSKLSKIYPNVEFIKIEKLKLILLCKYRNQIIDLEDDKK
                     RLLLLKLLYLTSKQFCYDEFKGAKFGCMSFFSHYQGYMSRLDSLDSDKFVSVKQRIVS
                     SISKLVEQYSDHIINLDRTLVDSLSDTLENQLFDDWDYILSEITTYSELLAKSGLLYN
                     SLDYKIDNVKKEVAKLSDFHQSLFKIEGFSDFFDSWQFQRYRSLVNQVYEIFSMLQVS
                     SVERTVLCGALSTIVFSKSNFNYQAFFEYLKDT"
     gene            23079..23987
                     /locus_tag="VQ620_001014"
     CDS             23079..23987
                     /locus_tag="VQ620_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763659.1"
                     /translation="MDYVDVILDEYGSVFLIKDHIKLKINNLELYRYFSSFGMSHLQF
                     RKEELPGKLKQFDFEKNKSDSYFSKKSSLIDFIKRNYFYDIKLYSDNPKNPIESWKNK
                     TEKIKIAKSTEILWSEVANFSSISKSPYYCLLYNLKNTENYILIISKTYFSAIQKMVS
                     YNLNNYFELEDVCCKSIVSKRELIENSININKSKMKLLNVCEGSKGYTRRVFKYNNIF
                     VGQLEFHSNIIFSEIALNELSIVKLLDSVESVLSEQLFGKLNWDNLFVVLSDDFNDYL
                     DINESQKYYSNGSLRGLELGIFMGGV"
     gene            23989..25887
                     /locus_tag="VQ620_001015"
     CDS             23989..25887
                     /locus_tag="VQ620_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HET2763660.1"
                     /translation="MRIIEIVDVEDQLLQLLSAHTNIDIRAQELSTLDPDSKVDLIIY
                     LKSRRDIENINNIIIKSPNRNIIVIISAFDIIYALYFPKSTFRPCLNCLLLKLKDNNP
                     EHNLLLFLKYGTLPSKHFNLDNRLKSNLILFNEKLDPKVEEYSIEGVNTTNFTFHFSC
                     SCENSGYDMSERMADILLGEGKKLSPKNYRLKKSEHLFDFTRYLDRNFGFFHHSYDEK
                     ISEYAPLHAVEINVDAGYESGWGRTFMTEAATKSAFLESLERYCNSRNRRYKKLIRGC
                     FSDLSKKYNTLNPVDFCLSNKEYRDSSYKFHDFDSEKNYDWIFGKNITTGKICLLPSQ
                     LVFYKDFSKSEENERFVYETSNGCALGSSIEEAIFYGILEIIERDNFLLTWYLKEEVK
                     QLSWESLPSRLRIINERLKEDNCELKLFNITKEVGIPVIWALYINHNENASIRSFSAA
                     GCHINPLSAIESAIFEVITSVPTFERLIEVRNKESFIEEIQNNFSLIRDIDDHILYHA
                     HADYKNKMPFLFKNNYSEYQNEFDENRIFNTYSHATLTSDLQALIKIIEKKFGDIYVV
                     NISDKVSEKFGLYCVKVLIPGLLNMSFGHSNRRVNIERIKNYVDYRGGNVNSITLNSI
                     NHLPHPFP"
     gene            25911..>26390
                     /locus_tag="VQ620_001016"
     CDS             25911..>26390
                     /locus_tag="VQ620_001016"
                     /inference="COORDINATES: protein motif:HMM:NF025394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HET2763661.1"
                     /translation="MKRDWKSKHLFYHDVQQFDKLVIYLSKFMDKLVNQNQIEKYFYI
                     RYWEGGPHIRLRYLVRDTQNFETIDSMINKYAYKFWENNPPMKLLNKTDYKKIYNESI
                     DNNGKMDRKLRKNATIENIAYIPEIERYGGEEALIVSEDLFCICSQVSTALLNVVDNY
                     "
BASE COUNT         8435 a         4328 c         5604 g         8023 t
ORIGIN      
        1 tagtccgtct cgctccgtga ggtgcgagac aaaacaacca cccgctatgc gggtgcgcgt
       61 cgaagattat accaaaaaaa ctccaaacgc gatacaataa aggtgttcaa tccaattgta
      121 aagcgaaagg agaaaaaata tggcacaaaa gactcatagt ttatcataca caaagtggat
      181 gtgttctatc atattgtgtt cacccctaag tataaacgaa aagtcatcta taatcaatat
      241 agaagtagtt taggcgaaat atttcatcgc ttgtgtagtt ataaaggtgt tgaaatgatc
      301 gagggtcact taatgccaga ccatgtacac atgttagtca gtattccact aaggctaagt
      361 gtttcaagtt ttatgggtta tttaaaaggc aaaagtgcac tcatgatgtt tgacaaacac
      421 gccaatctca agtacaagtt tgggaatcgg catttctggg cggaaggtta ttatgtaagt
      481 acagtagggc ttaataaagc cacaattaag aaatatagtc aagattaaag aaattatcca
      541 gtggatgatt tcttcacgag tatgaaaatt tgagaacgag taaagcatga tatatagtaa
      601 aatgaaataa gaataggaca aaatcgatca ggacagtcaa atcgatttct aacaatgttt
      661 tagaagcaga ggtgtactat tccagtttca atctactata gcactagata aattgagtgt
      721 aaaagaatat gaggatccct ttagggatag tggtaagtaa taccaaagtc tcttaaagag
      781 gcaagtgacg agtcaagagc aataaggctt gaacaacgtg aaagccagcg tctttaggcg
      841 ctggctgatg atttgggctt atagctctga gataaaccac ccgttagaca ggtggttatg
      901 attttatctg agtgtaacat actgttgggc aatctcgctg atgcggtcaa agttgccttg
      961 ggaagcgagt ttattgagtt cgccaccaat tccaacggcg tctgcaccag cagcgaacca
     1021 ttgagggatg ttgtttagac cgactcctcc ggttaccatt acggaaactt gtgggatcgg
     1081 tgccttgact gcagagatat atgctggact gagagtacta cctgggaaga gtttgatgat
     1141 ttcactaccg gcttcaagtg cagtcgtgat ctctgtgagg gtaatacagc ctggaatgta
     1201 cggtgtgctg tagagattgc acattttcgc agtttcagca tggaaagatg gagaaacaac
     1261 gtaatttgct ccggctagaa tggcatctct agcagttacg gcatcaagca cagtacctgc
     1321 accgatacaa acactctgat cgtcctgata caagtctaca agttccttga tgatttgtcc
     1381 tgcatactga ttggtatagg cgatttcaat agctttgata ccgcccttga tacaagcaat
     1441 cgaggcttgc agtccttctt cctttgtatt tcctcgaatg acagcgacaa ttttctgttt
     1501 ttttagttca ataatcgtat ctgatttggt catgtaattc tcctaacgaa tgatatcttg
     1561 tgcatttgcc agtaaatttt caatactagt tgcggaagtg gagagatggt ctcctggaag
     1621 agtgcatttg agagttgcgc tcgcaactgc aaagtcaatg gtagttttta gggaggaatg
     1681 atggagtagt tggtaaaggg caccagatat aaaggcatcc ccgctaccaa ttcgttgata
     1741 gactgcagtt tttagttgga cagactcttc aaatcgttct tccagagcat aggcttgata
     1801 gacatttttg tcttgttcat cactagatcg gagggtatgg aaaatggcct tgaaaccata
     1861 ggcttctttt aaaagtcgca tgcgattttc cacctcttct aggctagcac tgtctcttgg
     1921 aaacatctct agattttggt catcaatcat gagaggatca ataccgaagc aatagtcagt
     1981 aaaacgtgca aacttagaaa attcatactt ggcttctagg actgaaatca tctttgttct
     2041 cagattgaga tccattgaaa cgacaattcc tcggcgcttg gcttcttcca agagtaggag
     2101 aaggatcgca cggacctctt gaccgatagc tatggtgatt ccactaaaat gaaaatggct
     2161 aatcccctga aagagagaat ccatatctag catgtttggc cgaatctggc tgatactcgt
     2221 atgcttacga tcgtagaaaa cttcactttg acgacaacca aagccgttct ccaaatagta
     2281 gaggccgatt cgatcgccaa gccgacagat taaactggta tcgatttggt gctgtttcaa
     2341 gaatgtgaga aaacgatctc caatctcgtt ggcagggagt gcggtaaaga ctttcgttga
     2401 gatacccagg gcttgcaaat tacaagcgat gttaatttct gatccgccaa aataagtcga
     2461 actggcaaca tgatcgccga tactggtggc atctaatggt gagattcgaa ttaatggttc
     2521 gccaaacagt aatattttcg tcatccatta gttcctttct ggtttagaat agctttactt
     2581 ggctaatact tctttcaaat aagcagcgac ttcatcatct tggcagtttt caaagaagta
     2641 ttcttggaag tatttgccag agatggtgtc tttgaggaag tcttggtcga ttgttttaag
     2701 gatggtcatc aaatcattgt gggtgatttg tttcacctcg tttaagatac gagcgttgcg
     2761 ttgttcaggg attacacgtt ctcttgggta gccaccgccc atttcttcag caaataagcg
     2821 ttcaaacatc aatttgaggg tcagttctgc cccccagcca aatcctttgg cataagggat
     2881 agacaaggcg ttaccaccat tgatttgaga ataaaggtaa gcgtcagttg ggtccactgc
     2941 tagaccacag acaacaccag ggaagctgtt taaagcaagc atagccccta cacccgtacc
     3001 acagccggta acaacaaagt caactgcctt tgtatttaaa aggatggcag ccattagtcc
     3061 gttctgcaca taagttaatt gactttctcc ttcttctcca cgcataccat agttaaataa
     3121 ttggtagtct tttttatctg tcgcttcttt tagactatcg taaataatgt gattcttgct
     3181 agcttgacta ttttcattga ttaatgcgat tttcataaga tcttctttct atttttactc
     3241 aggttgtttt ccgatgtagg ctaagatacc gccatctaca tataggatgt ggccattgac
     3301 aaaactgctg gcatcactag cgagaaagac agcagggccc atcaaatctt caggatttcc
     3361 ccaacgtgca gcaggtgttt ttgcaatgat gaactggtca aatgggtggc gagaaccatc
     3421 ttcttgcaac tcacgaagag gtgctgtttg aggagtggca atataacccg gtccaattcc
     3481 gttacattgg atattggctc caccgtattc agacgcaatg ttgcgggtca acattttcaa
     3541 gcccccttta gcagcagcat aagcactaac tgtttcacgt cccagttcgc tcatcatcga
     3601 acaaatattg ataatctttc catgcccttt ctttatcata gaaggaataa ctgcctttga
     3661 aacgataaat ggtgcgttta agtcaatatc gatgacctta cggaaatcag cggcgctcat
     3721 ttcgcacatt ggaactcggc ggataatacc agcgttatta acgaggatgt caatgacacc
     3781 aacctcttgt tcgattttct tgaccatggc ttggataccg tcctcgtctg tcacgtcaca
     3841 gacatatcca tgggcttcga tgccaacttc acgataagct tcaatccctt tattgaccag
     3901 atcttgattg atatcgttaa agacaatagt agcgccggct tcagcgtagg atttggcaat
     3961 agcaaatcca attccataag aagcgccggt gatgagagca atttttcctt ttaaagaaaa
     4021 ctgctcaatt gagaatgatg tatttgtcat ttgatacttc ctttcaaatt tgttaagact
     4081 tctctataac caaattatat tctagtaaac cggttttgtc aataaataaa tatttttatt
     4141 tataaaaaga tttgtttcct aaaaaaacgg aagattttga aatttctgtc tattaaaatg
     4201 gttaaaaaga taatgaaagc gctaatattt ttttaaaaaa actattgaca agataaaaat
     4261 aaaggattac aatggggaat ataaagtaaa ccggtaaacc taaacaagaa aggagaaaag
     4321 atgaaaattg tacttgtagg gcatggacat tttgctacag ggatttatag ttctttacaa
     4381 ttgattgcag gcaatcaaga aaatgtggag gcaattgact ttgtggaagg aatgtcagca
     4441 gatgaactca agcaaaaaat cttacttgca atttcaaatg aagaagaagt tttaatccta
     4501 agtgatctct tgggaggatc gccattcaag gtttcttcta ccataatggg agaaaatcca
     4561 gccaaaacaa tgaatattct ctcgggtttg aacttagcca tgttaatgga agcagtcttt
     4621 gctagaatgg ctcatagctt tgatgaggtt gttaataaat cagtagtggc ggcccagggc
     4681 ggagtcgtaa atggtaaaga attgttttca acggatgcag aggaagagga agaagatttc
     4741 gaatcgggta tttaaagggt aaaagaatga taaaaaaggt tacgattgaa aaaataaaat
     4801 cgcctgagcg cttcttagaa gtaccacttc tgacgaaaga agaagtcggc caggcaatcg
     4861 ataaggttat tcggcagtta gaactcaacc ttgactattt caaggaagat ttcccgacgc
     4921 cagctacctt tgataatgtc tatccaatca tggataacac ggaatggacc aatggtttct
     4981 ggacaggaga actgtggttg gcttatgaat acagtcaaca ggatgcattt aaaaacatcg
     5041 ctcataaaaa tgttctttct ttcctggatc gtgtcaataa gagagtagaa ttggctcacc
     5101 atgatctcgg cttcttgtac acaccgtctt gtatggctga atataagata aatggagatg
     5161 gagaggctag agaagcagcc ttgaaagctg cagataagtt gattgaacgc tatcaagaaa
     5221 aaggtggttt tattcaagct tggggagact tgggcaagaa agagcattac cgtttgatta
     5281 tcgactgctt gctcaatatc caactcttat tctttgctta tcaagaaaca ggtgatcaaa
     5341 aatactacga tattgcagaa agccatttct atgcttcagc taataatgta atccgtgatg
     5401 acgcttcgtc cttccacacc ttctattttg atcctgagac aggtcaaccc tttaaaggtg
     5461 taacgagaca agggtatagt gatgattcat gctgggcacg tggtcaatca tggggagttt
     5521 atggtattcc tttgacttat cgtcacttaa aagacgagtc ctgctttgac ttgtttaagg
     5581 gtgtgaccaa ttatttcttg aatcgtctgc caaaagatca tgtgtcttat tgggatttga
     5641 tttttaatga tggtagtgct caatcacgag attcttcagc aacagctatc gccgtctgtg
     5701 ggattcatga aatgctaaaa catctcccag aggtggatgc tgacaaagat atttataaac
     5761 atgctatgca tgccatgctt cgttccttga tcgaatatta tgcaaatgat caatttaccc
     5821 ctggtgggac aagtctcctc catggtgtgt actcatggca ttcaggtaaa ggagtggatg
     5881 aaggcaatat ctggggtgac tactattacc tagaagccct tatccgtttc tacaaagact
     5941 ggaacctata ttggtaggag gagaaatatg acaatgccaa atattattat gacccgtatc
     6001 gatgaacggt tgattcatgg acaaggacaa ctttgggtaa aatacctagg ttgtaatacg
     6061 gtcattgttg ccaatgacga agtaagcacg gacaagatgc aacaaactct gatgaaaaca
     6121 gttgtgccag actcagttgc catgcgtttc ttccctttgc aaaaggtgat tgatatcatt
     6181 cacaaggcta atcctgctca aacgatcttt atcgttgtaa aggatgtgaa ggatgcttta
     6241 accttggtag aaggtggtgt ccctatcaaa gaaatcaata ttgggaacat tcacaatgct
     6301 actggtaaag agcaagtgac acgctccatc ttcctgggtg aagaggacaa ggcggccctc
     6361 aaggaattga gccaaactca tcaagtaaca tttaatacga aaacaactcc aacaggaaat
     6421 gatggagctg ttcaagtcaa cattatggac tatatttaaa agaggagatc gttatgtcga
     6481 ttaatgtatt tcaagcgatt ttaattggat tatggacagc tttctgtttt agtggaatgc
     6541 tgttaggaat ttacaccaat agatgtattg ttctgtcatt tggtgtcgga attattctag
     6601 gtgatctgcc tactgctctt gcaatgggag ctattggtga attggcttat atgggattcg
     6661 gtgttggtgc tggaggtact gttccaccaa acccaatcgg acctggtatc tttggtacct
     6721 tgatggctat cactagtgct ggtaaagtca gtccagaagc ggctcttgcc ctctctactc
     6781 cgattgctgt ggcgattcaa ttcttacaaa ctttcgccta cactgtacgt gctggtgcgc
     6841 ctgaaacggc gatgaagcac ttgaaaaacc ataatttgaa gaaattcaag ttcactctaa
     6901 atgcaacaat ttggttgttt gcctttattg gatttacctt gggctgcttg ggtgcccttt
     6961 caatggatac cttgttgaaa ctcgtagact acattccacc ggtattgctt acaggtttga
     7021 cagttgctgg taaaatgctc ccagctatcg gttttgcgat gatcttgtca gtgatggcta
     7081 agaaagagtt gattcccttt gtcttgttgg gatatgtttg tgcagcttat ctaaacatcc
     7141 caacaattgg tattgcaatt gtaggtacta tctttgcttt gattgaattt tataacaagc
     7201 caaaaacagc ggatcatgtg gtagaggagg aagcacacga tgactggatc taacaaatta
     7261 acaaaacgtg attatcttaa aacgtctttg cgggcattct tttgtcaaaa tggatttaac
     7321 tatagtaact atcaagggtt gggatatgcc aatgtgatgt atcctgcttt gaaaaaacac
     7381 tatggagagg atcaggaagg tttctaccaa gccttggaag aaaactgtga attctataat
     7441 accaacccac acttcctgcc ttttattacc agcttgcatc ttgtaatgtt ggaaaatggc
     7501 cgtccggcaa aagaaacacg tagcatcaag atggccttga tgggaccatt ggcaggtatt
     7561 ggggattctc tttctcaatt ctgtttagct ccattgttct caaccatcgc agcttcgttt
     7621 gctcaagaag gcttggttgt cggtccaatc ttgttcttcc ttgcgatgaa tacgatttta
     7681 acagcgatta aattgtcaac tggtctgtat ggatacaaac taggaacaac tgtgattgat
     7741 aaactaagcg aacagatggc aacgatttct cgtattgcca atattatcgg tgtaaccgta
     7801 attgctggtt tggcagcgac atctgttaag attatggtgc cgattacctt tgctgcaggg
     7861 gaagttaaag cagacgctaa acaaagtatc gtaagtattc agggaatgct tgataaggtt
     7921 gctccagctc ttctaccagc cctatttaca cttttagttt actacttgat caaagaaaag
     7981 aaatggacaa catataaact cgttatttta acagttatca tcggaattat cggaagctgg
     8041 cttaagatta tagcttaatg gatacaacat tgttttatgg aatagtgatt gtcttggcag
     8101 tgagtcccct tttactgtca agctttcatt ctattcgtca gcaaaagttg cttcgcaaac
     8161 agatggagca acgacaagag tatttagctt ctttaacatc tggtgatgaa gtgttgttat
     8221 tgtctggaat tcatggaaaa atcatttcta tcaaagatga cttggtctcc ttgcagattg
     8281 caaaaggtgt ggtcatctat gtagaaaagg aaagtgtaat gggaaagaca aaagaactgc
     8341 tttttaagta gttctttttc tggtaataga taatgaaaga agaaagaaga caattttttg
     8401 aaagaatcga tggaaaccaa tgtcgtgatt atatcttgtc ccactgttca aaagactatg
     8461 agaaagtcaa gagttccctt gaacgcttga tggacaatcg ttttatgttt gatagtcctt
     8521 gggatatgga gccttgttca aaaatccatc aaatccagcc gatggtatgg gatcaagtat
     8581 ttgaagatga tccagaatgg tcttatatgc tcaatcgaca agaatatctc ttgcagttta
     8641 tgatagggta tctggtagaa ggagataagg actatattca aaagtgcaag ttctttctat
     8701 ttgattggat tgagcaggtg agagaatttt ctcctcaatc cttgatgact agaaccttgg
     8761 atacgggtat tcgttccttt acttggttga aactactctt gcttctcttg aaatttgact
     8821 tgctagagga gaaagaacta gagaaaattt tggtcagtct agaaaagcag attgacttta
     8881 tgaaaagcta ctatcgcgcc aagtacaccc ttagtaactg ggggatttta caaacaattc
     8941 cgatgcttgc tatctatcat ttcttttcag ataagatgga cctagaagaa gcttaccatt
     9001 ttgcttcaga ggagttgaaa cagcaaattg agacacagat tttaggagat ggaagccagt
     9061 ttgaacagtc gattctctat catgtagagg tttataaagc cttgctggat ttgtgtctct
     9121 tgcttccaga cttgcaagat agttaccaag agttgctgga aaagatggcg acctatattc
     9181 aaatgatgac aggcttagat ggacgaactt tggcttttgg tgatagcgat tctacagaaa
     9241 cgacagaaat tttgagcctg tctgctgtgg ttttgaacaa ggaagacctt cttaacggtc
     9301 tggatgttaa agttgatttg cttagcctct tgttcctggg gcgagaaaag gtcaagcgac
     9361 tgcaggaatt tgaaaggaga gcttggcagc ctaagtccat gatctttgaa gactctggac
     9421 atgtctgtat taaggatgaa catcgttatc tatttttcaa aaatggtccg ctaggaagtg
     9481 cccatagcca tagtgacgag aatagttttt gcttacagta tcaaggccaa cctattttca
     9541 tagatgctgg gcgttattct tatcgggaga tatatgaacg ttatctctta aagagtgctt
     9601 ggagtcattc gacctgcatt gtagatggga aagctccgga aagaatcaca ggatcctggg
     9661 aatatgaata ctatcctcac tccctgtttt gtcaccataa agaaagggag ggagtgcatt
     9721 atattgaggg ggcttattgg tcagcagaac ctgatttgcc ttatcttcac agaagaaaaa
     9781 tcctcatgtt ggtagaggat gtctggctct tggtggatga catcaggtgt caaggtcagc
     9841 atgaggcgtt gactcagttt atccttgaca aggatgtgac ctatcaagat gggaaaatca
     9901 atcagttgag actatggagt gaagttgatt ttgatttgga agataccatc atttctccta
     9961 aatacaatga gcttgaaaga agtagcaaac tcaccaagcg ccaattcttt gagaatcaga
    10021 tgctggatta taccatcatt gcgcatgaga gttttgaaat catccgtcat tctgtctacc
    10081 agacagatga tcgtgaagtg gaaaatgctc tggcttttga agtgaaaaat gacgaaacag
    10141 acaagctgat tctgttatta agcgaggata ttggtgtagg tgaaaaattg tgcctcgttg
    10201 acggaacaaa aatgcgtgga aaatgtctag tatatgataa aataaatgag agaatgattc
    10261 gcttgcagtg ctagaaatag gcattttgaa tagtgaacat gttataataa gtattagtag
    10321 gaggtgtttt agattggaga agaaactgac cataaaagac attgcggaaa tggctcagac
    10381 ctcgaaaaca accgtgtcat tttacctaaa cgggaaatat gaaaaaatgt cccaagagac
    10441 acgtgaaaag attgaaaaag ttattcatga aacaaattac aaaccgagca ttgttgcgcg
    10501 tagcttaaac tccaaacgaa caaaattaat cggtgttttg atcggtgata ttaccaacag
    10561 tttctcaaac caaattgtta agggaattga ggatatcgcc agccagaatg gctaccaggt
    10621 aatgatagga aatagtaatt acagccaaga gagtgaggac cggtatattg aaagcatgct
    10681 tctcttggga gtagacggct ttattattca gccgacctct aatttccgaa aatattctcg
    10741 tatcatcgat gagaaaaaga agaaaatggt cttttttgat agtcagctct atgaacaccg
    10801 gactagctgg gttaaaacca ataactatga tgccgtttat gacatgaccc agtcctgtat
    10861 cgaaaaaggt tatgaacatt ttctcttgat tacagcggat acgagtcgtt tgagtactcg
    10921 gattgagcgg gcaagtggtt ttgtggatgc tttaacagat gctaatatgc gtcacgccag
    10981 tctaaccatt gaagataagc atacgaattt ggaacaaatt aaggaatttt tacaaaaaga
    11041 aatcgatccc gatgaaaaaa ctctggtatt tatccctaac tgttgggccc tacctctagt
    11101 ctttaccgtt atcaaaaagt tgaattataa cttgccacaa gttgggttga ttggttttga
    11161 caatacggag tggacttgct tttcttctcc aagtgtttcg acgctggttc agccctcctt
    11221 tgaggaagga caacaggcta caaagatttt gattgaccag attgaaggcc gcaatcaaga
    11281 agaaaggcaa caagtcttgg attgtagtgt gaattggaaa gagtcgactt tctaaaatga
    11341 aggaaaatga cttgcaatct ctgttaagaa ataaaataat cccacctaga acaagctagg
    11401 tgggattatt tgcctatgaa atgagaaatt atgggagcaa gctcctaaat caactgtttt
    11461 tgatctactt ctttaactac ttgataaaag ttatagaagt aggccaaact tgaaatgatg
    11521 gttacgacta ggaatattga aaatttccat tggacagggg tggttaaaag ttgtggaaag
    11581 gatatgagga gaaagaagag ggctgcgttg aggacaggta tccgttttga ttgtattttc
    11641 tcaagtcctt tattgagcgc aggaagaaag aggagtagga gtagtaaaac tgtatgagaa
    11701 acagctcctg aaataagggc gaagaaaagg aaaatactga taaaaacatg aatgatcagt
    11761 agtctagcta gtgatttcat aaggcacctc ctaatcctgg tcttttttag ctcttgcaat
    11821 acgaagtgag tcgacaatat gtatcatcac tccgaaaaag aaagctccca gtatagtttt
    11881 aaaaatatgt tttgtattta gaagagaact gataaaattt ggattttcac ttgttagggt
    11941 atcaatgagt ggaattataa aaaatatcac tgttccataa atcgaacctg ctttcagacc
    12001 aggataacgt aactgtttct tttctttttt catgagtttc ctcctaatcc tcatcttgat
    12061 ttttcttagt ttttgcaatg cgacgggaga tgaggaactg tatgctcgct ccgaagaaaa
    12121 tagaaccgag aacacttgat acaccatttc ttatagtgag aagagaatga aaatagtcct
    12181 gaccttcatc tatgagtata ctgagaagag gagttataaa aaacatccat agaccaaaga
    12241 acaaacctgc tttcagacct gggtagtgta gttgcttgct ttctttctca ttcagcatat
    12301 ctggttcaat gactgtgatg cctgtttttt tcatttggta ggtgacatag ccagcagcga
    12361 tgagggcaat cactaaaatc agaggaggat agattagagc cacttcttga gggtatttat
    12421 aggccagaag gagtggaata agatttccga aaatcatcag ataaaagagg atgataaaaa
    12481 cttggttccc aatactatcg gcctcacgcc gtttgtattc gtcaagggga ccagaaatac
    12541 cgtatgtgcg tttgatcagt ttttcagtga aggtttcttt tttcataagt ttgctccttt
    12601 tttaaaaatc ttcctcccaa aagagactgt tgaggtcagt ttggaggctg cgggcgagat
    12661 tgagacagag ttccaaggtt ggattgtact tgtcgttttc aatcatattg atagtctgtc
    12721 tcgagacacc gatatccttg gcgagttcga gctgggaaat acccaattcc ttgcgaaatt
    12781 ctttcacacg attcatctgt tctcctttct gatttatgtc gtatatattt gactatatta
    12841 tagtctttta aacataaagt gtcaagtatt tttgacatat tttttgaaga aatagtagtc
    12901 tccttgtcct atttgtctga caagtgcaag ctggtcggat ttgtggtaaa atagataaga
    12961 tatgacaaaa gaatttcatc atgtaacggt cttactccac gaaacgattg atatgcttga
    13021 cgtaaagcct gacggtatct acgttgatgc gactttgggc ggagcaggac atagcgagta
    13081 tttattaagt aaattaagtg aaaaaggcca tctctatgcc tttgaccagg atcagaatgc
    13141 cattgataat gcgcaaaaac gcttggcacc ttacattgag aagggaatgg tgacctttat
    13201 caaggataac ttccgtcatt tacaggcacg tttgcgcgaa gctggtgttc aggaaattga
    13261 tggaatttgt tatgacttgg gagtgtctag tcctcaatta gaccagcgtg agcgtggttt
    13321 ttcttataaa aaggatgcgc cactggacat gcggatgaat caggatgcta gtctgacagc
    13381 gtatgaagtg gtgaacaatt atgactatca tgacttggtt cgtattttct tcaagtatgg
    13441 agaggacaaa ttctctaaac agattgcgcg taagattgag caagcgcgtg aagtgaagcc
    13501 gattgagaca acgactgagt tagcagagat tatcaagttg gtcaaacctg ccaaggaact
    13561 caagaagaag gggcatcctg ctaagcagat tttccaggct attcgaattg aagtcaatga
    13621 tgaactgggg gcggcagatg agtccatcca ccaggctatg gatatgttgg ctctggatgg
    13681 tagaatttca gtgattacct ttcattcctt agaagaccgc ttgaccaagc aattgttcaa
    13741 ggaagcttca acagttgaag ttccaaaagg cttgcctttc atcccagatg atctcaagcc
    13801 caagatggaa ttggtgtccc gtaagccaat cttgccaagt gcggaagagt tagaagccaa
    13861 taaccgctcg cactcagcca agttgcgcgt ggccagaaaa attcacaagt aagagggaaa
    13921 aagatggcag aaaaaatgga aaaaacaggt caaatactac agatgcaact taaacggttt
    13981 tcgcgtgtgg aaaaagcttt ttacttttcc attgctgtaa ccactcttat tgtagccatt
    14041 agtattattt ttatgcagac caagctcttg caagtgcaga atgatttgac aaaaatcaat
    14101 gcgcagatag aggaaaagaa aaccgaattg gacgatgcca agcaagaggt caatgaacta
    14161 ttacgtgcag aacgtttgaa agaaattgcc aattcacacg atttgcaatt aaacaatgaa
    14221 aatattagaa tagcggagta agatatgaag tggacaaaaa gagtaatccg ttatgcgacc
    14281 aaaaatcgga aatcgccggc tgaaaacaga cgcagagttg gaaaaagtct gagtttatta
    14341 tctgtctttg tttttgccat ttttttagtc aattttgcgg tcattattgg gacaggcact
    14401 cgctttggaa cagatttagc gaaggaagct aagaaggttc atcaaaccac ccgtacagtt
    14461 cctgccaaac gtgggactat ttatgaccga aatggagtcc cgattgctga ggatgcaacc
    14521 tcctataatg tctatgcggt cattgatgag aactataagt cagcaacggg taagattctt
    14581 tacgtagaaa aaacacaatt taacaaggtt gcagaggtct ttcataagta tctggacatg
    14641 gaagaatcct atgtaagaga gcaactctcg caacctaatc tcaagcaagt ttcctttgga
    14701 gcaaagggaa atgggattac ctatgccaat atgatgtcta tcaaaaaaga attggaagct
    14761 gcagaggtca aggggattga ttttacaacc agtcccaatc gtagttaccc aaacggacaa
    14821 tttgcttcta gttttatcgg cctagctcag ctccatgaaa atgaagatgg aagcaagagc
    14881 ttgctgggaa cctctggaat ggagagttcc ttgaacagta ttcttgcagg gacagacggc
    14941 attattacct atgaaaagga tcgtctgggt aatattgtac ccggaacaga acaagtttcc
    15001 caacgaacga tggacggtaa ggatgtttat acaaccattt ccagccctct ccagtccttt
    15061 atggaaaccc agatggatgc ttttcaagag aaggtaaaag gaaagtacat gacagcgact
    15121 ttggtcagtg ctaaaacagg ggaaattctg gcaacaacgc aacgaccgac ctttgatgca
    15181 gatacaaaag aaggcattac agaggacttt gtttggcgtg atatccttta ccaaagtaac
    15241 tatgagccag gttccactat gaaagtgatg atgttggctg ctgctattga taataatacc
    15301 tttccaggag gagaagtctt taatagtagt gagttaaaaa ttgcagatgc cacgattcga
    15361 gattgggacg ttaatgaagg attgactggt ggcagaatga tgactttttc tcaaggtttt
    15421 gcacactcaa gtaacgttgg gatgaccctc cttgagcaaa agatgggaga tgctacctgg
    15481 cttgattatc ttaatcgttt taaatttgga gttccgaccc gtttcggttt gacggatgag
    15541 tatgctggtc agcttcctgc ggataatatt gtcaacattg cgcaaagttc atttggacaa
    15601 gggatttcag tgacccagac acaaatgatt cgtgccttta cagctattgc taatgacggt
    15661 gtcatgctgg agcctaaatt tattagtgcc atttatgatc caaatgatca aactgctcgg
    15721 aaatctcaaa aagaaattgt gggaaatcct gtttctaaag atgcagctag tctaactcgg
    15781 actaacatgg ttttggtagg gacggatccg gtttatggaa ccatgtataa ccacagcaca
    15841 ggcaagccaa ctgtaactgt tcctgggcaa aatgtagccc tcaagtctgg tacggctcag
    15901 attgctgacg agaaaaatgg tggttatcta gtcgggttaa ccgactatat tttctcggct
    15961 gtatcgatga gtccggctga aaatcctgat tttatcttgt atgtgacggt ccaacaacct
    16021 gaacattatt caggtattca gttgggagaa tttgccaatc ctatcttgga gcgggcttca
    16081 gctatgaaag actctctcaa tcttcaaaca acagctaaag ctttggagca agtaagtcaa
    16141 caaagtcctt atcctatgcc tagtgtcaag gatatttcac ctggtgattt agcagaagaa
    16201 ttgcgtcgca atcttgtaca acccatcgtt gtgggaacag gaacgaagat taaaaacagt
    16261 tctgctgaag aagggaagaa tcttgccccg aaccagcaag tccttatctt atctgataaa
    16321 gcagaggaag ttccagatat gtatggttgg acaaaagcga ctgctgagac ctttgctaag
    16381 tggctcaata tagaacttga atttcaaggt tcgggctcta ctgtgcagaa gcaagatgtt
    16441 cgtgctaaca cagctatcaa ggacattaaa aaaattacat taactttagg agactaatat
    16501 gtttatttcc atcagtgctg gaattgtgac atttttacta actttagtag gaattccggc
    16561 ctttatccaa ttttatagaa aggcgcaaat tacaggccag cagatgcatg aagatgtcaa
    16621 acagcatcag gcaaaagctg ggactcctac aatgggaggt ttggttttct tgattacttc
    16681 tgttttggtt gctttctttt tcgccctatt tagtagccaa ttcagcaata atgtgggaat
    16741 gattttgttc atattagtct tgtatggctt ggtcggattt ttagatgact ttctcaaggt
    16801 ctttcgtaaa atcaatgagg ggcttaatcc taagcaaaaa ttagctcttc agcttctagg
    16861 tggaattatc ttctatcttt tctatgagcg tgggggcgat atcctgtctg tctttggtta
    16921 tccagttcat ttgggatttt tctatatttt cttcgctctt ttctggctag tcggtttttc
    16981 aaacgcagta aacttgacag acggtgttga cggtttagct agtatttccg ttgtgattag
    17041 tttgtctgcc tatggagtta ttgcctatgt gcaaggtcag atggatattc ttctagtgat
    17101 tcttgccatg attggtggtt tgctcggttt cttcatcttt aaccataagc ctgccaaggt
    17161 ctttatgggt gatgtgggaa gtttggccct aggtgggatg ctggcagcta tctctatggc
    17221 tctccaccaa gaatggactc tcttgattat cggaattgta tatgtttttg aaacaacttc
    17281 tgttatgatg caagtcagtt atttcaaact gacaggtggt aaacgtattt tccgtatgac
    17341 gcctgtacat caccattttg agcttggggg attgtctggt aaaggaaatc cttggagcga
    17401 gtggaaggtt gacttcttct tttggggagt gggacttcta gcaagtcttc tgaccctagc
    17461 aattttgtat ttaatgtaag aatggcatcc tgatgtttca gggtgttttt gtatttaaat
    17521 acacaatgaa aatcaaagaa caaactagaa agctaacttt aggctgctca aaatatagta
    17581 gattgaaact agaatagtac acatctactt ctaaaatatt gttagaaatc gatttgactg
    17641 tcctgatcga tttgtcctgt tcttatttca ttttaatata tagttttgag atggttgata
    17701 gaactgacga agtcagctca aaacatggtt ttgaggttgt agataaggag aagctgatgt
    17761 ggtttgaaga ggttttcgaa gagtgtaaaa aaatattggt cagttaaagc caaaactctt
    17821 gaccttttct gaccaatgta gtatattatg aacgtaaggt aaatgaaagc cttactagaa
    17881 cacttattta aagtaaaata gaaagaggtg ggtctatgtt tcatctagaa atcttcagaa
    17941 gtaaagatgg tctactcctg cttgaaaaag aaaaaccaga aatagtacgt agggtagcgg
    18001 tttaactagt ctaaattttt taaaacaatg gtcaaagata gtcaatatca gtaatcataa
    18061 ctaagtaaac aaaaagaggt aaagaatatg aataacaact ttaataattt taataacatg
    18121 gatgatttat ttaaccaatt gatgggtggt atgcgaggat atagttctga aaatcgccgt
    18181 tacttaatta atggacgcga agtcacatct gaggaatttg ctcactatcg tgcgactggt
    18241 caattaccag gaaatgcaga aactgatgtg caaatgccac aacaggcatc aggtatgaaa
    18301 caagacggtg tccttgcaaa actaggtcga aacttgacag cagaagcgcg tgagggcaag
    18361 ttggatcctg ttatcggacg aaacaaggaa attcaagaaa catctgaaat cctctcacgc
    18421 cgcaccaaga acaatcctgt tttggtcgga gatgcaggtg ttggtaagac agcagttgtc
    18481 gaaggtctag cgcaagccat tgtgaacgga gatgttcctg ctgctatcaa gaacaaggaa
    18541 attatttcta ttgatatctc aggtcttgag gctggtactc aataccgtgg tagctttgaa
    18601 gaaaatgtcc aaaacttagt caatgaagtg aaagaagcag ggaatattat cctcttcttt
    18661 gatgaaattc accaaattct tggtgctggt agcactggtg gagacagtgg ttctaaagga
    18721 cttgcggata ttctcaagcc agctctctct cgtggagaat tgacagtgat tggggcaaca
    18781 actcaagacg aataccgtaa caccatcttg aagaatgctg ctcttgctcg tcgtttcaac
    18841 gaagtgaagg tcaatgctcc ttcagcagag aatactttta aaattcttca aggcattcgt
    18901 gacctctatc aacaacatca caatgtcatc ttgccagacg aagtcttgaa agcagcggtg
    18961 gattattctg ttcaatacat tcctcaacgt agcttgccag ataaggctat tgaccttgtc
    19021 gatgtaacag ctgctcactt ggcggctcaa catccagtaa cagatgtgca tgctgttgaa
    19081 cgagaaatcg aaacggaaaa agacaagcaa gaaaaagcag ttgaagcaga agattttgaa
    19141 gcagctctaa actataaaac acgcattgca gaattggaaa agaaaatcga aaaccacaca
    19201 gaagatatga aagtgactgc aagtgtcaac gatgtggctg aatctgtgga acgaatgaca
    19261 ggtatcccag tatcgcaaat gggagcttca gatatcgaac gtttgaaaga tatggctcat
    19321 cgcttgcaag acaaggtgat tggtcaagat aaggccgtag aagctgtagc tcgtgctatc
    19381 cgtcgtaacc gtgctggttt tgatgaagga aatcgcccaa tcggcagctt cctctttgta
    19441 gggtctactg gggttggtaa gacggagctt gctaagcaat tggcactcga tatgtttgga
    19501 acccaggatg cgattatccg tttggatatg tctgaataca gtgaccgcac agctgtttct
    19561 aagctaattg gtacaacagc aggctatgtg ggttatgatg acaatagcaa taccttaaca
    19621 gaacgtgttc gtcgcaatcc atactctatc attctcttgg atgaaattga aaaggctgat
    19681 cctcaagtta ttacccttct cctccaagtt ctagatgatg gtcgtttgac agatggtcaa
    19741 ggaaatacag taaacttcaa gaacactgtc attattgcga cctcaaatgc tggatttggc
    19801 tatgaagcca acttgacaga agatgcggat aaaccagaat tgatggaccg tttgaaaccc
    19861 ttcttccgtc cagaattcct caaccgcttt aatgcagtca tcgagttctc acaattgact
    19921 aaggaagacc tttctaagat tgtagatttg atgttggctg aagttaacca aaccttggct
    19981 aagaaagaca ttgacttggt agtcagtcaa gcggctaaag attatatcac agaagaaggt
    20041 tacgacgaag tcatgggggt tcgtcctctc cgtcgcgtgg ttgaacaaga aattcgtgat
    20101 aaggtgacag acttccactt ggatcattta gatgctaaac atctggaagc agatatgaaa
    20161 gatggcgttt tggttattcg tgagaaagcc taagacagaa ttttgaggat aaaaaagaag
    20221 gagccagctg aaaaaactgg ttccttttga ggtacgacgg gcatgtcgta tagtagaagt
    20281 gtactattct agtttcaatc cactatagta gctcagaagt cggtacttaa acgtgctata
    20341 tcaaaaccag tccttgaaaa acgtggattg gtttcgtgtt tggattatta ccttgaacga
    20401 catgcgttaa aagttagttg aaccgccgta tgccgaacag cacgtacggt ggtgtgagag
    20461 gggctagaga ttatccccta ctcgattgtc tatagctata ggggtaaagt tattagagag
    20521 gttgctagtt tatagagaca tcttgatttg taaaaagtta aattttagac aattatcccc
    20581 tttaaaaaat ctattaaacc atagctagtt gaagtaaatt tttatagaac ataattttat
    20641 ttatcaagac attgtagagt tgaggcaaat tgaaaaagaa agtttaaaag ttgataataa
    20701 gtcaataaga aagctattaa ttctaacgag attatttgat agtaatttgt tttttcagct
    20761 actattcgga caatgttatt ataataaaca caggagagaa caccagaagg tagggacatc
    20821 agaagaaata gttcaggata tagttaacat aatcatcaaa gaagctccta gactcgctaa
    20881 tgttaatttg gaaacatatt atccagatga atatatgtca aatacattga agcaattact
    20941 actttttaga gaggagttca taaaaaatat tttgaatggg attgatgtgg atgaaacagt
    21001 agtaatagaa aatgattttg tagaagagtt ggagaacttt ttaaaagaga atgatttgct
    21061 agatttttca aatagcatat tttttcagtt atctggagat aaagtaatag tgaataatat
    21121 ttatccagga tatttagcat attttaatag gttccttaca ttttatccga aaatattcgc
    21181 tattaaagaa gttggagaat atattgactt tttaaatttt gaaaagagaa ttgcggatat
    21241 gttttattgt tggggattta atgcaaattc tagagtgttg acagctgaaa aaataattga
    21301 aatcccaaat caccaaatca atgctaagta cataaaaaaa gttataaaaa tgagtgaact
    21361 cttgtttggt gttgatagta ataacagaat tcggttgctt aacaatagaa gggaattgat
    21421 aaaaactatt ttccaaggtt ctgtaattaa gtcagcaatt cctgctctag ctgctacttt
    21481 ggatacaatc agtaacagtg gatcatttca aatttcttta tcaaagcaga taattaataa
    21541 tttagtatta agtggtaaag ataaagttac aattcctagg atagagttag ataatgttgt
    21601 tctttcaaga cagaagatta ttgtatcagt ttcgtatata aatgattttt tggatttgag
    21661 atttccagat aaaatagaat acattttaaa ttttttagaa ggatctggtt tcaatactaa
    21721 agtaatggtc tactttggga aaattgtaga agaagataag aactattttg ttaatcttga
    21781 aaaacctcag tattttgatt ttaccagtat acttttcaat aagttattct ttaaagaatt
    21841 aaacaagttt gattacatgg ttattgaaga actcattcca aatcttgatg aagatgtaat
    21901 gatgacagag tatataaaag agtatactga ataggagcaa tctattatga ttttaagtaa
    21961 agtacgttgt tacatatata attctaaaat tagaggtaaa gttctgtatg aattatatga
    22021 atttataaat gaaaataagc aattgatgga cgcaattaat tcaattaaag ttgacagtag
    22081 taatggtttt agtattcaaa tattacattt aggagaacag caaacaactg tcttattaac
    22141 agattttttt actaaaactt tagacttgtt taaggaattt agggttacaa ataagagtac
    22201 tcttgagcag gtgaaaaatc aaattagcat actaaattct tctggtccaa aaatggattt
    22261 aacgaatata aaaacagatt actcaatagt ggaagaaaaa ttagattggc aaagtaaatt
    22321 atctaaaatt tatccgaatg ttgaatttat aaaaattgaa aaattaaaat tgattttgtt
    22381 gtgtaaatat agaaatcaaa ttattgactt agaggatgat aaaaaaagat tgttactatt
    22441 gaaattatta tatttgactt caaagcaatt ttgctatgat gaatttaaag gagcgaaatt
    22501 tggatgtatg tcattttttt cacattatca aggttatatg agcagattag attcactcga
    22561 ctcagataaa tttgtttcgg ttaaacagag gattgtttcc tctatttcta aattagttga
    22621 gcaatattca gatcatatta taaatctgga tagaacttta gttgattctc taagtgatac
    22681 ccttgagaat cagctatttg atgattggga ttatatatta agcgaaataa ctacatatag
    22741 cgaattatta gcaaaatcag gattgttata taattcgcta gactataaga ttgataatgt
    22801 aaaaaaagaa gtggccaagc taagtgactt tcaccaatct ttatttaaaa ttgaaggatt
    22861 ttcagatttt tttgatagtt ggcaatttca aagatatagg tctctcgtga accaagttta
    22921 tgaaattttt tcaatgttac aagtaagtag tgtagaaaga actgtgttgt gcggagctct
    22981 aagtactatt gtctttagta agtcaaattt taactatcaa gctttttttg agtatctaaa
    23041 agatacgtag aattcgattt taaaggaggc tgaaatatat ggattatgta gatgtaatat
    23101 tggatgagta tgggagtgtt tttctgataa aggatcacat aaaactaaaa attaataatc
    23161 tagaactata tcggtatttt agtagctttg gaatgagtca tcttcaattt agaaaagaag
    23221 agcttccagg aaaattaaag cagtttgatt ttgaaaaaaa taaaagtgac agttattttt
    23281 ctaagaaaag tagtctgatt gattttatta agagaaatta tttttatgat attaagttat
    23341 attctgataa tccgaaaaat ccgatagagt cttggaaaaa taagacagaa aaaattaaga
    23401 ttgctaaaag tacagaaatt ctttggagtg aagtagcaaa tttttcgtca atttctaaat
    23461 ctccttacta ttgcttgtta tataatttaa agaatactga aaattacata ttgataatca
    23521 gtaagacata tttttcagcc atccaaaaaa tggtttcgta taatctaaat aattattttg
    23581 aattagaaga tgtttgttgt aaaagcatag tgagcaagcg agaattgata gaaaattcaa
    23641 ttaatattaa caaaagcaaa atgaaactgc taaatgtttg cgaaggtagt aaaggttaca
    23701 caagaagagt atttaagtac aataatattt ttgtgggaca attggaattt cactctaaca
    23761 taatcttttc agaaattgct ttaaatgaat taagtattgt taagctttta gattctgttg
    23821 aaagtgtttt atctgagcaa ctatttggaa aattaaattg ggataattta tttgttgttt
    23881 tatcagatga ttttaatgat tatctggata taaatgaatc tcaaaaatac tactcaaatg
    23941 gttctttgag aggactggag cttggaatct ttatgggagg ggtttaaaat gagaataatt
    24001 gaaattgttg atgttgagga ccaactttta caacttttaa gtgcacatac caatattgat
    24061 attagagcac aagagttatc aacacttgac ccagattcaa aagttgattt gatcatatac
    24121 ttgaaatctc gacgtgatat tgaaaacata aataatatta ttattaaatc acctaacagg
    24181 aatatcattg ttataataag tgcttttgat attatatatg cattgtattt tccaaaatca
    24241 acatttagac cttgtttgaa ctgtctatta ttaaaactta aagataacaa ccctgagcat
    24301 aatctattat tattcctgaa atacggaaca ttacctagta aacattttaa tcttgataat
    24361 aggttaaaat caaatttaat actatttaat gagaagttag accctaaggt agaggagtat
    24421 agtattgagg gggtaaatac tacaaatttt actttccatt ttagttgtag ctgtgaaaat
    24481 agtggttacg atatgtcgga aagaatggca gatattctac ttggtgaagg aaaaaaatta
    24541 tctcctaaaa attatcgttt aaaaaagagt gagcatttat tcgattttac tagatatcta
    24601 gatagaaatt ttggattttt tcatcattca tatgatgaaa aaatctccga atatgcgcct
    24661 ctccatgcag tagagattaa tgttgatgct ggttacgagt caggctgggg aaggactttt
    24721 atgacagagg ctgcaacaaa atctgctttt ttagagtcat tagagagata ctgcaactct
    24781 agaaatagac gctataaaaa attaattaga gggtgttttt ctgatttatc taaaaagtac
    24841 aatacattaa atcctgttga tttttgtctt tctaataaag aatatagaga tagttcctat
    24901 aaatttcatg attttgattc tgagaaaaat tatgattgga tatttggtaa aaatataact
    24961 accggaaaaa tttgcttatt gccctcgcaa ctagtatttt ataaagactt tagtaaaagc
    25021 gaagaaaatg aaaggtttgt ttatgaaaca tcaaatggat gtgctttggg gagtagtatt
    25081 gaggaagcta ttttttatgg gattttagag attattgaaa gagataattt tttacttaca
    25141 tggtatttaa aagaagaagt aaaacaatta tcatgggaat ctttaccatc tcgcctacgt
    25201 attattaatg aaagactaaa agaagataac tgtgagctca aactttttaa tataactaaa
    25261 gaagtgggca taccagtaat ttgggcattg tatataaatc ataatgaaaa tgctagtatt
    25321 cgaagttttt cagctgcagg ctgtcatatt aatcctttaa gtgcaattga atcagcaatt
    25381 tttgaagtaa taacttctgt gccaactttt gaaagattaa ttgaagttag aaataaagag
    25441 tcatttattg aggaaattca gaataatttt tctttaatac gagatattga cgatcatata
    25501 ttgtatcatg ctcatgcgga ttataaaaat aaaatgccct ttttatttaa aaataattat
    25561 agtgaatatc aaaatgaatt tgatgagaat agaattttta atacttattc acatgcaacg
    25621 cttacatcag atttacaggc actaataaaa attattgaaa aaaaatttgg agatatatat
    25681 gtagtgaata taagtgataa agtttccgaa aaatttggac tttattgtgt taaagtgttg
    25741 attcctggac ttcttaatat gtcatttgga cattcaaatc ggcgtgtaaa tatagagagg
    25801 ataaaaaatt atgtagatta tagaggggga aatgtaaata gtattacatt gaattccata
    25861 aaccatcttc cccatccatt tccataagat tatatataaa ggaataataa atgaaaagag
    25921 attggaaatc aaagcattta ttttatcatg atgttcaaca atttgataaa ttggtaatct
    25981 atttatcaaa atttatggat aaattagtta atcagaatca gattgaaaaa tatttctata
    26041 ttcgttattg ggaagggggg cctcatattc gattaagata tttggtgagg gatacacaaa
    26101 attttgaaac aattgatagt atgataaata agtatgctta taaattttgg gaaaataatc
    26161 ctccaatgaa gttattaaat aagaccgatt ataagaaaat ttataatgag agtattgata
    26221 acaacggaaa gatggataga aaattgagga aaaatgcgac tattgaaaac attgcctata
    26281 taccggaaat agagagatat ggcggggaag aagctctgat agtaagcgaa gatctatttt
    26341 gtatctgtag tcaggtttca actgcgttac ttaatgtggt agataactat
//
LOCUS       DAVBGY010000023        24913 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVBGY010000023 DAVBGY010000000
VERSION     DAVBGY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24913
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.023"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            470..1591
                     /gene="mnmA"
                     /locus_tag="VQ620_001017"
     CDS             470..1591
                     /gene="mnmA"
                     /locus_tag="VQ620_001017"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET2763662.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1732..2187
                     /locus_tag="VQ620_001018"
     CDS             1732..2187
                     /locus_tag="VQ620_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2763663.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELDVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            2197..4110
                     /gene="mnmG"
                     /locus_tag="VQ620_001019"
     CDS             2197..4110
                     /gene="mnmG"
                     /locus_tag="VQ620_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET2763664.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            4299..4388
                     /locus_tag="VQ620_001020"
                     /pseudo
     CDS             4299..4388
                     /locus_tag="VQ620_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4534..6213)
                     /locus_tag="VQ620_001021"
     CDS             complement(4534..6213)
                     /locus_tag="VQ620_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET2763665.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(6215..6448)
                     /locus_tag="VQ620_001022"
     CDS             complement(6215..6448)
                     /locus_tag="VQ620_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HET2763666.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6830..7051
                     /locus_tag="VQ620_001023"
     CDS             6830..7051
                     /locus_tag="VQ620_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763667.1"
                     /translation="MTNQVLEENYLCDSLLAFSPPRDLCRLVCILLWIKCLDALLMLG
                     FFVWGCRDSRFTTRKFHPIYQDEVVFAFF"
     gene            complement(7264..7416)
                     /gene="cibB"
                     /locus_tag="VQ620_001024"
     CDS             complement(7264..7416)
                     /gene="cibB"
                     /locus_tag="VQ620_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HET2763668.1"
                     /translation="MMKDLNNCREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(7418..7603)
                     /gene="cibA"
                     /locus_tag="VQ620_001025"
     CDS             complement(7418..7603)
                     /gene="cibA"
                     /locus_tag="VQ620_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HET2763669.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            7781..8464
                     /gene="tsaB"
                     /locus_tag="VQ620_001026"
     CDS             7781..8464
                     /gene="tsaB"
                     /locus_tag="VQ620_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET2763670.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMESLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLTHTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMPEAHLSFEEVLEKVKGASQVTFVGEVGPF
                     VEQIQEHLPRTNYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            8461..8898
                     /gene="rimI"
                     /locus_tag="VQ620_001027"
     CDS             8461..8898
                     /gene="rimI"
                     /locus_tag="VQ620_001027"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET2763671.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            8888..9898
                     /gene="tsaD"
                     /locus_tag="VQ620_001028"
     CDS             8888..9898
                     /gene="tsaD"
                     /locus_tag="VQ620_001028"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET2763672.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(9944..11244)
                     /locus_tag="VQ620_001029"
                     /pseudo
     CDS             complement(9944..11244)
                     /locus_tag="VQ620_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11520..11871)
                     /locus_tag="VQ620_001030"
                     /pseudo
     CDS             complement(11520..11871)
                     /locus_tag="VQ620_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(11978..12270)
                     /locus_tag="VQ620_001031"
                     /pseudo
     CDS             complement(11978..12270)
                     /locus_tag="VQ620_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            12513..13719
                     /locus_tag="VQ620_001032"
                     /pseudo
     CDS             12513..13719
                     /locus_tag="VQ620_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            13712..14360
                     /locus_tag="VQ620_001033"
                     /pseudo
     CDS             13712..14360
                     /locus_tag="VQ620_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            14370..15986
                     /locus_tag="VQ620_001034"
     CDS             14370..15986
                     /locus_tag="VQ620_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2763673.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLSGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
     gene            16006..16850
                     /locus_tag="VQ620_001035"
                     /pseudo
     CDS             16006..16850
                     /locus_tag="VQ620_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813546.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16918..18419
                     /locus_tag="VQ620_001036"
                     /pseudo
     CDS             16918..18419
                     /locus_tag="VQ620_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
     gene            18431..19180
                     /locus_tag="VQ620_001037"
     CDS             18431..19180
                     /locus_tag="VQ620_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET2763674.1"
                     /translation="MSDNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSKLKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            19193..20365
                     /locus_tag="VQ620_001038"
     CDS             19193..20365
                     /locus_tag="VQ620_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET2763675.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGTLP
                     SKNEELMKFFCENNLNITFFDTFSEIKNNIDYYIIALPTDYDEKIGSFNTYEIEQTVS
                     KILRVKPNGKIILKSTVPIGFSNKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVV
                     GDETVEGRKIAELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELD
                     SFAERRSLNAEVVIKGVCLDPRIGNFYNNPSFGFGGYCLPKDTRQLKKEFIEINAPVI
                     EAIDISNTNRKQFIVKQILERKPKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIK
                     ILVYEPNLNVSIDNVIFEKNFELFIKQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
     gene            20760..21623
                     /locus_tag="VQ620_001039"
     CDS             20760..21623
                     /locus_tag="VQ620_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET2763676.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACAGLTASQLSKFELGQSM
                     LSADKLILAIQGINMNFDEFGHKLNNYQESPHMRIGRRVVDCFSHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKNALHSLDKSYPLTEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRWYMKSGYLNIISELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVLWQFLKKMQIFLQTKGSNQKEIEHFIQSLQVLENPQLTALFE
                     LRLQQYKELID"
     gene            21909..22142
                     /locus_tag="VQ620_001040"
     CDS             21909..22142
                     /locus_tag="VQ620_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763677.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            22164..22841
                     /locus_tag="VQ620_001041"
     CDS             22164..22841
                     /locus_tag="VQ620_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2763678.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATSLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            22853..23518
                     /locus_tag="VQ620_001042"
     CDS             22853..23518
                     /locus_tag="VQ620_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2763679.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFFVYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            23590..24816
                     /locus_tag="VQ620_001043"
     CDS             23590..24816
                     /locus_tag="VQ620_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2763680.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQILQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
BASE COUNT         7679 a         4105 c         5228 g         7901 t
ORIGIN      
        1 ttaggtgccc ttgcagtcaa cacaactgta gatggctata aagtcaatgc caatggtgaa
       61 tgggtttaag ccgattaaat taaatcatgt taagaacatt tgacatttta attttgaaac
      121 aaagataagg ttcgattgaa tagatttatg ttcgtattct ttaggtacct atcttatgat
      181 ttcaggaaat gtcattaaaa aaacgactcc ttttctctaa cttgaaaaat agattagaga
      241 aaatgggttg ttttatttat tatagttatt tgaatgaaga taagaagaag gtatactcac
      301 atcattcaca taatctgtat gttgactata agtttaaaaa aacaattttt aagctcttcc
      361 ttgtcttctc caaccaagcg tgttataatg aatactgctc aagcgacctt caatcgtgaa
      421 gcacacacga ccttcaatcg tgaataaacg aatagatggg agacttacca tgagtgataa
      481 ctctaaaaca cgtgttgtcg tggggatgag tggtggtgtt gattcgtcgg tgacggctct
      541 tttgctcaag gagcagggct acgatgtgat cggtatcttc atgaagaact gggatgacac
      601 agatgaaaac ggcgtctgta cggcgaccga agattacaag gatgtggttg cggtggcaga
      661 ccagattggc attccctact actctgtcaa ttttgaaaaa gagtactggg accgcgtttt
      721 tgagtatttc ctagcggaat accgtgcagg gcgcacgcca aatccggacg ttatgtgcaa
      781 caaggaaatc aagttcaagg cctttttgga ctatgccatg accttggggg cagactatgt
      841 agcgactggg cattatgctc gagtggcgcg tgatgaggac ggcactgttc acatgcttcg
      901 tggcgtggac aatggcaagg accagaccta tttcctcagc caactttcgc aagaacaact
      961 ccaaaaaacc atgttccctc tgggacattt ggaaaagcct gaagtacgca gactagcaga
     1021 agaagcaggc ctttcgactg ctaagaagaa agactcgaca gggatttgct ttatcggaga
     1081 aaagaacttt aaaaactttc tcagcaacta cctgccagct cagcctggtc gcatgatgac
     1141 tgtggatggt cgcgatatgg gcgagcatgc aggtcttatg tactatacaa tcggtcagcg
     1201 tggcggactc ggtatcggtg ggcaacacgg cggtgacaat gccccttggt tcgttgtcgg
     1261 aaaagatcta agcaagaata ttctctatgt aggacaagga ttctaccatg attcgctcat
     1321 gtcaactagc ctagaagcca gtcaagtcca ctttactcgt gaaatgccag aagagtttac
     1381 gctagaatgt acggctaaat tccgttaccg tcagcctgac tctaaggtga ccgtccatgt
     1441 caaaggagac aaggcagaag ttatctttgc ggaaccacaa cgcgcgatta caccaggaca
     1501 ggcagttgtc ttttacgatg gcgaagagtg tctaggtggc ggtttgattg acaatgctta
     1561 ccgcgatgga caagtttgtc agtacattta gattgacaaa ttttctcaat ttgctacaat
     1621 aataacagca atagaaatga tggtcaaagc tcatggatgt tgcaggcttt tttgtcctgc
     1681 acttctttgt agttttgact gtttttgtgt cgtttaaggg aaaggacaag aatgactcaa
     1741 caagactttc ggacaaaagt agacaatacg gtttttggtg ttcgggctac agccttgatt
     1801 gtccaaaatc acaagcttct agttaccaaa gacaagggca agtattacac tattggaggt
     1861 gcgattcaag tcaatgaaag cacggaagac gcggtagtcc gtgaagtgaa ggaagaactg
     1921 gatgtcaaag ctcaagctgg gcagctagct tttgtggttg aaaatcgttt tgaagtggac
     1981 ggcgtttcct atcacaatat cgagtttcat tatctggtgg atttgcttga agatgccccg
     2041 ttgaccatgc aggaagatga gaaaaggcag ccctgtgagt ggattgactt ggataagctc
     2101 cagaatatcc agctagttcc agccttttta aaaacagccc taccagattg ggaaggccaa
     2161 ctaagacaca ttcatcttga ggaataggag agaaacatga cttatcattt tactgaagaa
     2221 tacgatatta ttgtaattgg tgcgggacac gctggggttg aggcttcctt ggccgctagc
     2281 cgtatgggct gtaaggtcct gcttgcgacc atcaatattg aaatgctggc tttcatgcct
     2341 tgtaatccct ctatcggtgg ttctgctaag gggattgtcg tacgtgaagt cgatgccctc
     2401 ggtggcgaga tggccaagac cattgacaag acttacatcc agatgaagat gctcaacaca
     2461 gggaagggac cagccgttcg tgcccttcgt gcgcaggctg ataaggaact ttactctaag
     2521 gaaatgcgca agacagttga aaatcaagaa aatctgaccc ttcgtcaaac catgattgat
     2581 gagattttgg tggaagatgg caaggttgtc ggtgtgcgta cagccaccca tcaagaatat
     2641 gctgctaagg ctgttattgt gacgacaggg actgctctcc gtggggaaat tatcatcgga
     2701 gacctcaagt actcatcagg tcctaaccac agcttggctt ctattaacct agctgacaat
     2761 ctcaaggaac tgggtctcga aatcggtcgt ttcaagacag gaacccctcc acgtgtcaag
     2821 gcttcttcta tcaattacga tgtgacggaa attcagccag gagacgaagt gcctaatcat
     2881 ttctcataca cttcacgtga tgaggattat gtcaaagatc aagtgccatg ctggttgacc
     2941 tataccaatg gtaccagtca tgagattatc caaaacaacc tccaccgtgc gcctatgttt
     3001 acaggtgtgg tcaagggagt ggggcctcgt tactgtccgt cgattgaaga caagattgtg
     3061 cgctttgcgg acaaggaacg tcaccaactc ttccttgagc cagaagggcg caatactgag
     3121 gaagtctatg ttcaaggact ttcaaccagt ctgcctgagg atgtccagcg tgacttggtt
     3181 cattccatca aaggtttgga aaatgcagag atgatgcgga caggttatgc tattgagtat
     3241 gatatggtct tgcctcatca gttgcgtgcg actttggaaa ccaagaaaat ctcaggtctc
     3301 ttcactgctg gtcagacaaa tggaacatca ggttatgaag aagctgctgg ccaagggatt
     3361 atcgcgggta tcaatgcggc tctgaaaatc caaggtaaac ctgagttgat tctaaaacga
     3421 agtgacggtt atatcggggt gatgatcgac gacttggtga ccaagggaac cattgaacct
     3481 taccgtctct tgaccagtcg tgctgaatac cgtctcattc ttcgtcatga caatgctgat
     3541 atgcgcttga ctgagatggg acgcgagatt ggccttgtgg atgatgaacg ctgggctcgt
     3601 tttgaaatca agaaaaatca atttgataat gagatgaaac gcctagacag tatcaaactc
     3661 aagccagtca aggaaaccaa tgctaaggtt gaggaaatgg gcttcaagcc gttgacagat
     3721 gcggtgacag ccaaggaatt ccttcgccgt ccagaagttt cttaccaaga tgtggtggcc
     3781 ttcatcggac cagctgcaga agacttggat gacaagatta tcgaattgat tgaaacagaa
     3841 atcaagtacg aaggctatat ttccaaagcc atggatcagg ttgctaagat gaaacgtatg
     3901 gaagaaaaac gcattccagc caatatcgac tgggatgaca tcgattctat tgcgacggaa
     3961 gctcgtcaga agttcaaact catcaatcca gaaaccatcg gccaagccag ccgtatttcg
     4021 ggagtaaacc cagcagatat ttctattttg atggtgtatc tggaaggtaa aaatcgtagt
     4081 atttctaaaa ctcttcaaaa atcaaaatga tacgtcgtcg gcttcttacg aatgagttca
     4141 aagcttggct ttgattcatc tccaacctcc catagttccc cgaactatgg gagctaactc
     4201 aagttatgca ttttcctagc cttctggtct gattttcatt gagttttaat ctcaaaagaa
     4261 agcatagaga aaaacagctc cgaagacggg gctgttttgt tgacttgtac tcaatgaaaa
     4321 tcaaagagca aactaggaag ctagccgtag gttgctcaaa gcactgcttt gaggttgtag
     4381 atagaactga cgaagtcagc tcaaaacaca gttttgaggt tgtagataga actgacgaag
     4441 tcagctcaaa acacagtttt gaggttgtgg atagaactga cgaagtcagt aacatatata
     4501 cggcaaggcg aagctgacgt ggtttgaaga gtattattct tcatctggtg tgaggatcat
     4561 cgggatgatg atcggttcac gttcggtatt ttcatagagg aaggggcgaa tagcgttgac
     4621 aatggcacca ttgacagatt gcacgctagc atccttattt ttcagtgcga tacgaatggc
     4681 attgaagagg atacgctggc tttggcgaat caagtctcca gactctctca tgtagacaaa
     4741 gcctcggctg aggatatctg ggccagacag aatcatctgc gatttgaagt caacagttgc
     4801 gactgccaga acgacaccgt cttcagatag atcgcgacga tctttgagga cagctgcgcc
     4861 aatttcaccg atacgatttc catcgacata gatatcttgg gcgttgaaat gacctgcgat
     4921 acgagctgag tcagcagtaa gggcaagcac atcgccattg ctcatgataa agatattgtc
     4981 tttctcaaca ccagtatcca ctgctagtcc agcgtggact ttttgcatgc ggtattcacc
     5041 gtggacaggc atgaagtatt ttggcttaat caagcggagc atgagttttt gctcttgctg
     5101 accaccgtgt ccagatgtat ggatattgtt cactttaccg tggataactt cgacaccagc
     5161 ttcagaaatg atgttaatca gcttgttgac gctagtagtg tttccaggga tgggactaga
     5221 agagaagata acggtatcac ctggttgtaa ttgtacttga cggtgggttc cgttggcgat
     5281 acgagagagg gctgccatag gctcaccctg actacctgta cagaggataa gaacttctcc
     5341 tgcaggataa tctttgattt catttggctc gataaaggtt cccttaggag ctttgatgta
     5401 gccaagatcg attccgttga caatggcctt ttccatagaa cgaccaaaga ccgcaatctt
     5461 gcgtccagtc ttaacagcag cttctgttgc ctgctggaga cggaagatat ttgaggcaaa
     5521 ggatgcaaag atgatacgtc cttcaatacc ttggataatc ttcataatgg actgaccaac
     5581 gactttttca gagttggtaa aggttggtac ttccgcattt gtcgagtcag acaggagaca
     5641 gagcacgcct tcttcaccaa gcgcagccat acgatgcaag tccgcaggtt ctccaactgg
     5701 agtaaagtcg aacttaaagt cacccgtaca gacgattttc ccttgaggag tatgaatgac
     5761 aatccccaaa ggctctggaa tagagtgagt cgttctaaag aaagttgcct tgagattttt
     5821 aaaggtcaac tcggtgttgt ggttgatttc gtaaagtttg gcgttgcgca agaggccgtg
     5881 ttcttcgagt ttcccacgga tcaaagccaa ggcaagcggt ccagcgtaga tagggacatt
     5941 tgcttgcttg agtaggaacg gaatcccacc aatgtggtcc tcgtgtccgt gtgtgattaa
     6001 aacagccttg acgcggtcga tattgtctac gatgtaagag tagtcaggaa tgacatagtc
     6061 gataccaagc aagtcatctt ctgggaattt aatcccagca tcgacgatga taatctcgtc
     6121 ttggtattca attccgtaag tgtttttccc gatttctcct agaccaccga tggcaaaaac
     6181 accgacttct tcaggtttaa gagtgtaggc catattagaa ctccgtaatt tcgaaggcgc
     6241 cagtttcttt ttcgtaatcg agcaatttgt cagacaagag ttcgatatat tcgatattgt
     6301 actctgggcg attttcttcg acaagttggc gagcagtgat acggccctca agttctgagc
     6361 tggcatcgat gtctaggtaa agcgcgcgtg ttgtttcacg gcgtgggcta cgttcttttg
     6421 tttcttgata aaaaactttg taaatcatat agttcctttc tatttacagg tcagcttatt
     6481 ccaaactttt agcagatatt tgcttgattt cattccattt tgtgtctaga ttgaccttga
     6541 ttcgttacaa ttatttcaat tataccacaa aatgaaaaat tagtaaaaga cggaaacgag
     6601 aaagttaggg gacgatttct tcggtaagcg tttgcagaaa caaagaaaat gtgttatgtt
     6661 gataacggaa gtgaagttta ttacttgtca taattccata tagattttaa gtcaaggagg
     6721 ttgccctatg tataacttat ttgcttttgc tatcggagtt cgtttgctag cttttatcgt
     6781 ttttgttttg cgtttatgga ggaaatcttc tatttgccaa actggaacga tgacaaacca
     6841 agttcttgag gaaaattacc tttgtgactc tctactcgct ttttctcctc ctcgtgacct
     6901 atgtcgtttg gtttgtattt tactttggat taaatgctta gatgccctgc ttatgctggg
     6961 gttttttgtt tggggatgta gagatagtag atttacgaca cgaaaattcc accccatcta
     7021 tcaagatgag gtggtctttg catttttcta aaatgatatg acaatggaat tttcaaagct
     7081 tattattttt atgggagtca ttgttctgct caaaaataga gttgaagcat aaaaataaaa
     7141 caatacaaac agataaatcg aatggtaaag ggatggtagt aatgttcaaa tcattgagaa
     7201 tgctgagcaa actgtaagag atgaagacaa taaaatttat ccttctaaaa atgtgtttct
     7261 tatctagcat gacttaccaa actttttacg aaggtctttt ccaatggtat aacctgccat
     7321 aaataaagcg ataccaacac ctacaccaaa gccacccttg atttcttgca attccttatt
     7381 agaaatttca cgacagttgt tcaaatcttt catcatatta atctcctcct aaaaaataac
     7441 cagctgccaa accagaacct ataagtgctg ttccacctgc aatagctttc caagatactg
     7501 ctactccaaa gataaccaag ggagcgagac cgccatcaat atctgataat tcattttcag
     7561 ataaggataa aaattgattg tcaagaatgt caaaatttgt cattgaactt tcctcctttt
     7621 ttcttgttca cttttatatt cggaaaagaa aaggaaaagt gagcattttt ttaaattatt
     7681 tttagttaag tgagggcact cttgtctggg ggcaaagaaa attcccatgt gccatctttt
     7741 gtagtataat agtaagcaga caaaaagata ggaatgtgtt atgaaagtat tagcttttga
     7801 tacgtccagc aaggctcttt ctctcgctat tttagaggat aagcaggttc ttgccgagac
     7861 gacgattaat atcaagaaaa atcacagtat taccctcatg ccagccattg attttttgat
     7921 ggagagtttg gactggacgc ccaaggattt ggaccgaatc gtggtggcgg aagggccagg
     7981 tagctacaca ggcttgcgaa tcgcggtagc cactgccaag accttgactc atactctgaa
     8041 cattgagtta gttggtatgt ctagcctctt ggctctggtg ccatatcaac aagaaggctt
     8101 gttcgttcct ttgatggatg cgcgtcgcaa caatgtttat gcaggatttt atgaaaatgc
     8161 taaacctgtc atgccagaag cgcacctatc ttttgaagag gtgctagaaa aagtcaaggg
     8221 tgctagtcag gtaacctttg tcggagaagt tggccccttt gtggagcaga ttcaagaaca
     8281 cttgccaagg actaattata aagaaacctt gcctaatgca gccaatcttg ctcttttagc
     8341 ttgggacaag gaagcagact ccttgcatga ttttgtgcca aattacctca aacgagtcga
     8401 ggctgaggaa aactggctca agaaccatac cgagtctggt gagtcctaca ttaaacgcct
     8461 atgatagaaa tcaagcgaat tcaacaacag cctgacctag ctcaagccat ctacgctgtt
     8521 atggcagctg tttacctagt cagtccttgg actctggagc aaatccaagc agatctgtcc
     8581 caagaccaga cttggtatgc attggcttat gatggggcag aagtgattgg atttctagct
     8641 gtgcaggaga atctttttga agcagaagtc ctgcaaatcg ctgtcaaagg agcttatcag
     8701 ggtcagggaa ttgcgtcagc cttgtttgct caattgccga cagataagga gattttcctc
     8761 gaagtcagaa agtcaaatca acgagcgcaa gcattttaca agaaagaaaa aatggcggtc
     8821 atcgctgagc gaaaggccta ctaccatgac ccagtcgagg acgccattat catgaagaga
     8881 gaaatagatg aaggatagat atattttagc atttgagaca tcctgtgatg agaccagtgt
     8941 cgccgtcttg aaaaacgacg atgagctctt gtccaatgtc attgctagtc aaattgagag
     9001 tcacaaacgt tttggtggcg tagtgcccga agtagccagt cgtcaccatg tcgaggtcat
     9061 tacagcctgt atcgaggagg cattggcaga agcagggatt accgaagagg acgtgacagc
     9121 tgttgcggtt acctacggac caggcttggt cggagccttg ctagttggtt tgtcagctgc
     9181 caaggccttt gcttgggctc acggacttcc actgattcct gttaatcaca tggctgggca
     9241 cctcatggca gctcagagtg tggagccttt ggagtttccc ttgctagccc tcttggtcag
     9301 cggcggacac acagagttgg tttatgtttc ggaggcagga gattataaga ttgttgggga
     9361 aacccgtgat gatgcggttg gtgaggctta tgataaggtc ggccgtgtca tgggcttgac
     9421 ctatcctgca ggtcgtgaga ttgacgagct ggctcatcag gggcaggata tttatgattt
     9481 cccccgtgcc atgattaagg aagataatct ggagttctcc ttctcaggtt tgaaatctgc
     9541 ctttatcaat ctttatcaca atgccgagca aaagggagaa agcctgtcta cagaagattt
     9601 gtgtgcttcc ttccaagcag cagttatgga cattctcatg gcaaaaacca agaaggcttt
     9661 ggagaaatat cctgttaaaa ccctggttgt ggcaggtggt gtggcagcca ataaaggtct
     9721 cagagaacgc ctagcagccg aaatcacaga tgtcaaggtt atcatccccc ctctgcgact
     9781 ctgcggagac aatgcaggta tgattgccta tgccagcgtc agcgagtgga acaaagaaaa
     9841 cttcgcaggc tgggacctca atgccaaacc aagtcttgcc tttgatacca tggaataaag
     9901 agtggctctt tgtcaagtgt agtgggtagt cgaaaagctg caatctggag agtacgaaat
     9961 tcgtactctc ttttttgatg ttcaaagtca tcaaaatccg ttttttaaaa ttgttaaagt
    10021 tccggaaacc aaaggctttg cgcttgataa tcttaatcaa attattggtc gcttcaagtt
    10081 tagcgttgga ataaggcagc ttaagtgcgt tgataatctt atctctatcc cttaaaaaag
    10141 tccaaaagac cgtttgaaaa agcggatgaa ccgttggcag ttcttgctca atcaatccaa
    10201 agaaatggtc ggcattcttc tcttgaaaat gaaagagcag gagttgatag agttcgtagt
    10261 gaacctgtaa tccctcagag taagaaagca gcttatctcg tacttcccga tgggttaagt
    10321 gcattcgaaa cgtgggctga tagaatcgtt tctgacagag cttgaagcta tactggtgta
    10381 gtaaatcagt ccagtggact gatttagccc gagcctagaa acgaaaaagc gagggttcca
    10441 aaagcgcttg agcgcccgat actccaagga ttttcggtca aattggttca tgatttggat
    10501 tcgtactcgg ttcatagctc ggctcagatg ttggacaatg tggaagcggt caagaacaat
    10561 cttcgccttt ggaaagagtt gcttagcgag ccgataataa gggctgtaca tgtccatggt
    10621 gatgacctcg accagctccc gaacctctct ctgatagcgt tggaaatggt ttcgaatcac
    10681 cgcatgagtt cgcccgtcta aaattgcgag gatggatttg gactcaaaat cttgggcaat
    10741 gaagctcatt ttgctctttt tgaagctata ctcatcccaa ctcataactt ttggcaactt
    10801 ggtccaatcg gtttcaaact taaattccct cagttttcgg atgacggtgg aagttgagac
    10861 cgccaatctg tgggcaatct cagtcataga ctgcttttca aggaggagct gagcgatttt
    10921 ttgtctcacc atgttggaaa tctggcaatt tttcttgaca atggatgttt gagaaacgac
    10981 caccttaagg caattcttgc actgaaagcg gcgctttttg agatgcagta ccgtgggtaa
    11041 accctgacag tcgagaagcg gaattttaga ggctttctgg aagtcgtatt tgatcatctt
    11101 cccttgacaa tgaggacaag gaggagcggg ggaatccaac tttgcctgaa cgactagatg
    11161 ggtttggtat ttcagaacag acaagatttt gatatttttg tcttccaatc cgagcaaatc
    11221 tgtggtattc ttaagttgtt ccatatggac cctttctaat gagttggtta gacgcttttc
    11281 attataggtc atatggggct ttttttctac aagaaacgac cctataattc ctggggtggg
    11341 attacccact acagaaatta tagagccaaa gcattccaaa gtcttgtctg actctggaat
    11401 gttttctgtt cacagttgtt ttgtaactgc ctaataagta accgcgctat aacgaggtat
    11461 ctaccgagat tgtcaattct ccagtatact gttataaaaa acaaaactgg aggatttttt
    11521 tatgtcctcc catcgtccca tcgactgttc cgtagctgcc caataacgga gtatagtcgg
    11581 ctgaaacaag aataggacaa aagtgcctca agaaaagcat cgtaatttga caatattatt
    11641 ctgagatgtt tttgatgtaa gcccatattt tctcaatggg attatactcg ggtgaatagg
    11701 taggaagtgg taacagtcta tgccctgctc cttacacatg ttcattctgt gaaaccttgc
    11761 attgtccata atgataaggg atggtgtatc taaagtaggt agtaagaatt tttggaagca
    11821 agcttcgaaa aagtcactcg tcatcgtatc tttataggtc atcggagcca tagtatattg
    11881 aaacaaaaaa gggcattagt acttctataa cagtatcgca atctctggtg tgcgttgtct
    11941 cgatcttaat tcaaataatt atatcttctt ccagagagac taactgcctg ctcaaggctc
    12001 gatcatattt gagataaaaa taggtctcaa cccctgtctc ataaatataa ataggagtca
    12061 ggtagcttaa gtgattcaat tctttaagga accgatttac tttttctggg tcttgttcgc
    12121 agtaggtacg gctcttttta gactatatcc catagccttt agagcgtaat gaatagttgt
    12181 tggaggacag ttgaattcag cagctatttc agtcaaataa gcatcgggat gagtttcaat
    12241 atagatcttt aatttatctc tatcaacttt ttcaattaat agtggaagtt ttcttaaagt
    12301 tagtgtgttg aaacgttttt atacagtgct aaaatttgaa gtgtagtgca gatgtatttg
    12361 taatagtatt atagcaattc tttggagaag gtcgagacaa gagaaaatag gtatctttga
    12421 gatataagta tgatttacta taaaattatt gagttattca taaaaatttc tctatcaaat
    12481 atcataatgc gtcgtttaag gagatggtta caatgcaata tagttgtgga aaaataaata
    12541 ttaatatccc tgatggatat ggagacatta aggatatagt gttttcggct catattatag
    12601 ttaggtataa taatggacat tgtggaggta tagatccgca tataattgga ctttgtaaaa
    12661 agcagatcag aaggatgtct ccatatccta ttttgataat cgtatctaga gattcaaagg
    12721 ttattgatga ttataaaaat ttagatattg cctatgttga ttgtactcaa tgttcaaata
    12781 attttgaaac tgccttacac gttaaaaata ttttaaaatt attaaaaatc caacttatac
    12841 actgtcatgg ctactcaact aattattttt tatatatgtt aaaaaaactt gacaagaatg
    12901 gatttggaaa ggttaagaca gtgataacat gccatggatg ggttgaatat aacttaaaaa
    12961 agaaattctt gacttatttt gatttttgga catattctat gggagatgct tttatttgtg
    13021 tatctgaaac tatgaaaaaa agattggaga gtataataaa aaaataagaa gatagttgca
    13081 ataaataacg gaattaatgt atcaaatagc gatttagatg tagtaggtgt tcaagacttt
    13141 aaaaaagaat tttgtattcc aaataacaaa aaaatcattt gttatgttgg aaggttggat
    13201 ccagaaaaaa ggccggatag attccttgaa tttgcagaaa aattattttt agtcagagaa
    13261 gatgtgattt ttattatggc tggaaatggt agtatgtggg ctactttgaa agaaaaaatt
    13321 tgccatttga aatgtagaga taatttcagg ttacttggtg aaatctatcc agctactata
    13381 gtttaccaaa tatcggattt attgtatatt ccttctgata cagaaggaat acctatgtgt
    13441 gttttagagt caatgtcaca agaaactccg gttttagcta gtaatgttgg cgggttaagt
    13501 gaaattattg aacatagggt tgatggattt ttatttgaga aggaagatgt tgagggagtg
    13561 tgtgcttgtg ctaatttttt actcaatgat tctgagtatt tgaaatatat aggtgagaat
    13621 agtaaatcaa aaataagaaa acatttttct gtgcaaaaaa tgtttgtaga aaccatgaga
    13681 gtatatgatg aattattaga gaagagtagt catggatagg aatatagatc aagaattagt
    13741 aagtattata ataccaacac ataataggta tgaatctctg attagagctg tgaaaagttg
    13801 tttacatcaa agttataaaa atatcgaagt tataattatt gatgataatt atagtaatgt
    13861 aaatttaaga aataaaatta tccatcaatt tggctatacc aatcatagaa ttaagttaat
    13921 tttaagtaat gaagatttag gtgcaactaa tgccagaaac ataggtatca aaaattctag
    13981 aggtaagtat atatcatttt tagacgatga tgatgaatat atgccagatc gaattttaaa
    14041 gttgatggct tgttttaaaa agagtagaat gaagaattta gctttagttt atagttatgg
    14101 aataataatt tatccaatag gaaatatttt aatttattga gtttcaacga gagaatattg
    14161 gttacaaaga aatattattc tttaaacata aagcggttac tattaatagg agacaaatgc
    14221 aaggctttaa aagtaatcaa gaaggcaaga gaagaaaaaa tttttaacga atttcttttt
    14281 cgaaatatat gttattatat cattttggtt ttttctattg tatatatgac aactatgttc
    14341 aattaaaatt tagaaagtga gaaactattg tgtatactat tataaaatca aatataaaaa
    14401 aatttagttt attaacgata tttattgttg ctggtcaatt attgctaatt tatgcagcaa
    14461 ctattaatgc tctggtgttg aatgaattaa ttgcgatgaa tttagagcgg tttttgaaat
    14521 tgtcaatcta ccaaatgatt gtctggtgtg ggataatatt ccttgactgg gtagtgaaaa
    14581 attatcaggt tgaagtgatc caagagttta atctagagat tcgaaataga gttgccacag
    14641 acatctctaa ctctacctat caagaatttc atagtaaatc atcaggaaca tatctttcgt
    14701 ggctaaataa tgatgttcag actttaaatg atcaggcttt taaacaactt tttttagtaa
    14761 taaaaggaat ttctggtact atatttgcag ttgtgactct taatcactat cattggtcat
    14821 tgactgtagc caccttgttt tcattaatga ttatgctact tgtaccaaaa atctttgcat
    14881 cgaaaatgcg agaagttagt ctaaatttaa ctaaccaaaa tgaagctttt ttaaaatcta
    14941 gtgagactat attgaatgga tttgatgtgt tagcgtcctt gaatctttta tatgtattgc
    15001 ctaagaaaat taaagaagca ggaattttat taaagatggt tatacaaaga aagacaactg
    15061 tagaaacgtt agcaggcgct attagcttct ttctcaatat tttttttcag atatctctcg
    15121 tttttttaac aggctatctt gcaataaaag gaatagtgaa aattggtact attgaagcaa
    15181 taggagcact aacaggtgtt atttttacag cgctaggtga attaggaggt caattatcct
    15241 ctattattgg tacgaagcct atttttttaa aattgtattc aattaatcca attgagtcaa
    15301 ataaaatgaa tgatatcgaa ccaaatgagg tgaatagaga ttttccgtta tatgaagcaa
    15361 aaaatatttg ctataagtat ggagataaag aaatattaaa aaacttaaat ttttgttttc
    15421 aacgtaatga aaagtattta attttaggtg aaagtggaag cgggaaatct acattattaa
    15481 aattattgag tggctttttg agagattata gtggagaatt gcgattctgc ggggatgata
    15541 taaaaaaaac ctcctattta aatatggttt cgaatgttct atatgtagat caaaaagctt
    15601 atttgtttga aggtacgatt agagataata ttttattgga agaaaattat actgatgaag
    15661 aaatactaca gtctttagag caagttggtt tgagtgtaaa agattttcct aataacattt
    15721 tagattatta tgttggtgat gatgggagat tactgtcagg agggcagaaa caaaaaatta
    15781 ctttagctag agggctaatt agaaataaga aaatagtatt aattgacgag ggaacttctg
    15841 ctatcgatag gagaacttcg ttagcgattg aacgtaagat attagataga gaggatttga
    15901 ctgtcattat tgttacccat gctccgcatc cggaacttaa acaatatttt actaagatat
    15961 atcaatttcc aaaggatttt atttaagaat ataggggagg gaaacatgag attctattgt
    16021 gaagcttatg aggtaattac tgaagagaaa aaagtatact ttaatgatat tgagttagaa
    16081 gttaagtatt cctctgtcga agaattgttt ataatatgtg agaaattatt agataagaaa
    16141 aaagtatcat ttttttatgt atatgagaaa cccttaaggt atctgttatt tgactatata
    16201 tttctactag ttctagctaa aaaaaatatc ccaatcttag atggagtttc caataagcaa
    16261 gttgatccaa cgttgctgca tcacttttct ttggaaattg aaaagaattt tatcgatttt
    16321 tgctataaaa atatggatct catcctaaaa actcaaagta tatctttgtg tcatagagaa
    16381 gagcttatta ttgtagatgt ttcagatgac tctaaatttg gtgtttttta caagcgtttt
    16441 agaacactgg ttgataaaaa taacggaaaa tatgtttgtg tttataattt cagtcgtgtt
    16501 tcagatatac tgcaaaactg gaaaaattat tgcaatcgaa aattttcggt tacgtttact
    16561 gaaagccaat ttgaattatt taagctttta tataatcaaa aaaattttaa aacaatttcg
    16621 ttgctatttg gaaaaaaatt gttgctggag gaattatcta ttatagcgat cttactaata
    16681 tagaatactt ttgtattttt tggtgggata gtatgtttgg gaaagattct attggaaaat
    16741 acgtatatgt agaagagatt agtagatgtc attttttaga tagaaattat tctttttgtt
    16801 atggtttaca agattataaa agtaaactta ttaaatattt tttagaatag ttaatctatt
    16861 cgagagagat aaagaaatat ggaaaatact ttattgaata tcacaggaag cttcgatatg
    16921 gaaactagga atctaattag ttattcttta actgatatct ttgaaacaga taaaataaga
    16981 attgaacttc ttggagaaat ttattataaa aatataaagt tggaattgca tgaattcgct
    17041 ggattgtata agatttatgg aattagctta ataaagaata ttaccggaat gtttttaatt
    17101 ataatttttg atacgaagac aaaagaatta aaaatttttc aagatataac gacttcatac
    17161 tttaatttat actatactgt ttatggaggt gtgttctata tatagtacat ctttaaagaa
    17221 agtgatgaag ttatctcatg ttcccgttac cttaaataac aaaaaaatac aagaatttat
    17281 gagaaatgga tttattttag attcaaatac tttagtaacc gaaattaata aattagaata
    17341 tttctcttac atttctgtaa ataatactct tcgtatatgt ggtattgatt ataatgattc
    17401 aaacaatttt actaaagaac aagtattaaa gaattgggac tctatgctac gtgaatctat
    17461 attaagagtg tatagtgaag caggagaggc taatattaca ctatcatctg gttttgattc
    17521 aaattatata ttatatacat tagcgaatta tacgaattct tcaattaatg cattttgcat
    17581 aggaggagaa aaaggaatta atgagattcc tgaagtaact aaaattgcaa aattttatgg
    17641 tataaattta ttagttgata ctgtaatgag cccaagattt agaatatttt cccgatatag
    17701 tatggagatt ggaaggaagt ctctttgaat gtggtgtcat actgcagtac tatttaggca
    17761 ggttgttgtt ccaaaaaggg aacacaagta ttctttgtgg cgagtcagcg gatgaaataa
    17821 tgacttttaa gtatcattct gtgaactaca atcaattttg taatgataaa cagaagtcag
    17881 tatattttag ttattccgac tatccatttt atgttacaaa ttcaattgtt ttaaagaaaa
    17941 atagcctact tttgcatagt tttggcattc atcctagata tccatataaa atgtctgaga
    18001 ttgttgaaat gtctaaaaaa atatcagatc taaacgataa aaaagaattt cacaaaaaga
    18061 actgtgagtt gaggtttcaa gatagtgttc ttgataacat aaatagtgtc ccgggtacaa
    18121 ctcatttatt tagttgttta aatattcaaa cattagtaaa aattatttta tatatttttc
    18181 gatacaacag ttgtatgaaa atatttaatt ttagagataa agagcttatc ttttttaata
    18241 agattgtaaa tgggctgata caaaatattg aggagaattt agaggatgat attgaaagga
    18301 tattgaaata tttatatata tgtttattta acgaaatatt cattattaag aataaggtga
    18361 atttttttga tgatgtggaa tttaatcaga cacttagtga gtttttagat aaattgtaga
    18421 gggaatggtt atgagtgaca atactaagta tatcttttta tctccacatt tagatgacgc
    18481 aattttttcc tgtggtgatt atatttcaaa attaacttca gaaggagaaa ttgtacttgt
    18541 tattactata ttttctggtt accctttgag ccaacagcta cagccctctg ctaagcaatt
    18601 tcataagttg tgtaaccttg ggaaatatcc gattgaagaa aggaagaaag aagacaggtt
    18661 agcttgtgaa cgtctacaat gtgattttag acacttatcg tactatgaat gtctctatag
    18721 gaaagataga aatggcaatt ttctataccg tcatatctat agtaaattaa aaaatgagga
    18781 cacattaaag aatgatatca taaaagagct gttaatgcat ttagacgaca aatgtgtcgt
    18841 atattgtccg ctttcacttg gtgaccatgt tgatcatgtt tttgtgaata gtattggaag
    18901 ggcgcttgaa tttatgcgtt ataaagtaat atattatgag gacttcccat atgtaagtga
    18961 tagtagtatg gttagttata tgggaaaaac taaagaatta aaaatgtatc aagaggaact
    19021 tgatgaaaaa cattatattg atagaatatc atcaatattg tgctataaat ctcagatttt
    19081 gataatctgg aaatcagttg aaaaactttt aaacaatatt aaagagttgt atttaaggaa
    19141 tggggcagct tattctatta gattttggat taagaaataa ggaagtagtt ttatgaatat
    19201 agcagtaatt ggtttggggc atgttgggct ggcctacgcg ttactatttg catctaaata
    19261 taaagttgtt gcatatgata tagattctgt aaaaataaat aatttaaaaa agggcactct
    19321 tccatctaaa aatgaagagc ttatgaagtt tttttgcgag aataacttaa atattacttt
    19381 ttttgataca ttttctgaaa ttaaaaataa tattgattat tatattattg cgcttccgac
    19441 agattatgat gagaaaattg gtagttttaa tacatatgaa atcgaacaaa cggtatcgaa
    19501 gattctgagg gtaaaaccta atggaaagat tattttaaag tcaacagttc cgatcggctt
    19561 ttcaaacaaa ttaaaaaggc tgtttgatac aaaaaatatc atttttgtcc ctgaattttt
    19621 gagagaaggt tgttctatat atgataattt atatccaagt cgcatagttg ttggagatga
    19681 gacagttgaa ggaagaaaaa ttgcagagtt gttcctttcg attagtactc atagtactgc
    19741 caatattaaa aatgttatgt tagtttctcc tactgaagca gaagcaatta agcttttttc
    19801 taacacattc ttagctctcc gtgttgcttt ttttaatgaa ctagattctt ttgctgagag
    19861 gagaagttta aatgctgaag ttgtaataaa aggtgtttgt ttagatccaa gaattggaaa
    19921 tttttataat aatccttctt ttggatttgg aggatattgt cttcccaaag atactagaca
    19981 attaaaaaaa gaatttatag aaataaatgc cccagtgata gaagcgattg atatttcaaa
    20041 cacaaataga aaacagttca tagttaaaca aatattggaa cgaaagccaa agatagtagg
    20101 aatatataaa ttggggatga aatataattc agataattac aaagagtcag ctattttaag
    20161 tataattaat gaactattga ttgtcggtat aaaaatttta gtatatgaac caaatttaaa
    20221 cgttagtata gataacgtaa tttttgaaaa gaattttgaa ctatttataa aacaaagtga
    20281 tctaattgtt gcaaatagat gggatagggg ccttgaagct tataaagata aagtttacac
    20341 aagggggatc tggataagag attaactatt gatattaaaa aggcaataat ttataaaatt
    20401 tgtacaaaaa aatgttaaat tttcacattt tcatagtata ctgtttaata gaaagtaggt
    20461 tctttgtaac cgctccatta taaaatcctt tcactcttcc ggtatactat taggaaaaac
    20521 ataactggag gatattttga gctcacacct catcgttcca gtggctgttc cccaagctcg
    20581 tgtactgccc aaaaaagtta gacattctat ttagaacgag gattgagttc tgtattgtac
    20641 aaggctcggt ccttttagag tcagcttaag gctggctttt tcaatcacca aagtgtcaga
    20701 atgttttgac aaatgaacac aaataatgat ataatatgca aaagctagga ggtggtagga
    20761 tgattgaaaa aatggaactg ggggaatttt acaaggaatt gcgcttggcg agaaagctca
    20821 agcagtcaga tgtggcttgt gctggactaa cagcctctca gttgtccaag tttgaactag
    20881 gacagtctat gctgtctgca gacaagctga tcctagccat ccaagggatc aatatgaatt
    20941 ttgatgagtt tgggcacaaa ctcaacaact atcaagaatc tccacatatg cgaattggta
    21001 gaagggttgt ggattgcttt tcccatcagg atatagctgc tttagagcag ctattggagg
    21061 aagtcgatca agaacagatg gcgcagacct atcgtcgttt gaatgccatt gtgattaaaa
    21121 atgcactcca ttccttagat aaaagctacc cgctaacaga ggaggatagc gagtttttga
    21181 ctacctacct ctacgctatt gagtcttgga cctggtttga actctatctt ttttgcaata
    21241 ctatgccctt cttgagcaat caagatctga tctttttatc aacctcctta ctcgaaaaat
    21301 ccaaggaatt taaagagtta gtacacaatc gatggtatat gaaaagtggt tatcttaata
    21361 tcatctcaga acttatggag cgcaagctat tttcttatat tccaatcttt gaagctgagc
    21421 tggagcgcat gctccgtcca tacgatgttt ttgaaaaagt attgtggcag tttttaaaga
    21481 aaatgcagat ttttcttcaa accaaaggaa gcaatcaaaa agagattgaa cactttatcc
    21541 aatctctgca agtattagaa aatccacaat tgacagccct ttttgaattg cgtttgcagc
    21601 aatacaaaga gcttatcgat tagttagaaa tgggaaaaag ataataatat cagcaacgat
    21661 cgtcaataat gttaaaactt gtttcttcat attacttccc tcctcattag taataacttt
    21721 attataccag ggaaataatc aaatctacca aaatcgcaaa tctgaaattt ctataagaaa
    21781 aaatatcaaa tatgcgattt tctaaaataa gccaatttcc gtgttatact gtccttgtaa
    21841 aacatttgaa gcaaattcta ggtcgcagaa gtggtttaca aatgaagata attgaaggag
    21901 tgtaaaagat gttaaattta caatttgcag aaacaatgga attgacagaa gctgagttgc
    21961 aagatgttag aggaggcaac ctcgtaaatt ctatgggagg tggtggaaga agtggtatat
    22021 ctggttgggg cgtcccagga atttaccctg gttggggcaa tcaagggatg agtcctaatc
    22081 gtggagcttt tgattggact atagatttag cagatggatt gtttggccgc cgtagaagat
    22141 aggctcttat aggagataat aggatgaaga aaatgaaaga agtgaaattt cacctagcaa
    22201 ctggcttgtt gattttgact tattacctca tttttaatgt cacatcagac ttagatttta
    22261 tggttgcttt gtctgataat atgtactatg tgttccaagt tttattggta cttattctgg
    22321 gaactatagc tacaattgct tttgtcaaaa gtgaacattg gaaagagtgt ggtcgtttcc
    22381 agtttcgttg gtcttacctg ggtgttttcc ttctttcttt tttcttattg ttcgtgtggg
    22441 caaatctaac aacctatatc tttcctcgta cccagaatgg aagtacagtc gtagaagtag
    22501 ccaccagttt aacaggaatt agctattttg ttacgcgaat actttatact agtattatcg
    22561 cacctgtatc agaagaggtt gtttgtcgtg gtcttttgat gacaagttta tctaaagtta
    22621 agagatacta tcttgatgtt cttgtttccg ctgccatatt tggcgctatg catgttttgc
    22681 agtatggttg gataacgaca gatttcataa agtattttgg aatgggcttg atattttgta
    22741 tgatgtttcg ctacacccga tccatctatt gggctatagc tctgcatgct tcatggaata
    22801 gcttcttact cattgttact ttattggtat ttggatatta gaaaggtgat atatgaaaaa
    22861 gataatctca caccgctact ttattatagt ttttctacta gttattgctg accagaagtt
    22921 cagtgtttta gttttacgca gcgaccttgt tgctgggcta tctgactttg cctattatct
    22981 gtcggatatg atgttgaatt ttcttgtggt tttatttgct cttattgcta tgatttggtc
    23041 ggggaaatgg cagaaaatca atagtagaaa gtttaaagga tcctatcttt tctattcctt
    23101 cctagctctg cttgcttttg ttgcttggaa ttttgtcacc ttttttcttt tcccacctac
    23161 tcgaaatgaa atttcttatc aacatgctgc tcctactttt acaggagcta cggcattttt
    23221 gatgtatttt ttttatcctg tgattgcagg tcccattttt gaagacatga tttatcgtgg
    23281 attggtgatg acagctctgg aaaaaggaaa gaaatggggg ctagatgtac ttggttctgc
    23341 tattttattt ggggtctcgc acattagtaa tcacggttgg gtcttgacag actttttcgt
    23401 ctatatgggt ggcggactta tatttgcagt cttatttaga atgacaaagt ccatttattg
    23461 gcctattggg ctgcatatag tctacaacgg cattggtcag cttttgatgt tactgtaatt
    23521 ttggttgtta gtagtatcgc ggtctatttc tggggaaaga ttcccatttg aacttaagaa
    23581 ggagttgtga tgaaagtatt tcttcaaaat agagatttta ggcaattaac tatcaaccag
    23641 tggatttcaa cgcttgggga tacgattttt tatctggcct ttctgaatta tgtggcagat
    23701 gcgtcttttg cacctttggc gattttactc attacgattt cagaaaccct tccccaaatt
    23761 ttacaaatct ttctgggagt tttggcagat tttcaacatc atcgtgtctt gaaatacaca
    23821 gtcattagtt ttgcaaaatt tttgctttac tctatcgttt ctttatcact ttcagggcag
    23881 tctttctcct tgttgctagt agcatttatt tgtctgctta acctcttgtc tgacacattg
    23941 agttactttt caggagccat gctcactccg attttcatta gaattattgg gcaagaccat
    24001 ctggcagaag ctattggatt taaacagtca actgttagtt tagtgaaaac aatcagtaat
    24061 atcctaggag gagtcttgct agggattctc tccatccagt tcatttcctt gctgaacgct
    24121 cttacctttt taatcgcatt tttaggtatc ctcttcataa aaactgacct cttgaaagta
    24181 gaaaaaacga ttaactatca agaaggactc tctgtaaaat ccttttgcca gcatttgctc
    24241 caatcatcga aattgatatg gaatatgaat aaggtgctct tggttttgtt tattatctct
    24301 actagtcaag cagtgataaa tgttacagtt cctgtctcta ctctattttt gaggaatcaa
    24361 ccctttttga atttacaaac aggtcaatct cttgccttgc tatccacttt tgaactgtca
    24421 gccctgattg tcggaagcct tgtaagtggc tatctgcaac ataccatttc cataaaaaca
    24481 gctctctatg cctcacttgt catccagttg cttcttctag taggatttgc cacagttcgt
    24541 tttgactgga ttctcatctt tagtaccttg gatgcctttt tcgcaggtgt tctctctcct
    24601 agattgcaag aactcgtctt taaacaaata cctgaggagt caatgggagc agttcaatcc
    24661 tctatcggtg ccattacggt tgttttacct agcttattta caatagcttt ggtaaccatt
    24721 gctactagct ttggaactct ggcagttagc tttgttttat tgctatttct tctagttgcc
    24781 tttgttatgc ttctgaatat tcgtgaaagt atttagtgga gaatttctat aatatatgtt
    24841 tgagactgga ttgccagtct ttttatgatg gtcaaaatca acagactttt ttctcttttc
    24901 tgaatgtgtt ata
//
LOCUS       DAVBGY010000024        24118 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVBGY010000024 DAVBGY010000000
VERSION     DAVBGY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24118
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.024"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            7..735
                     /locus_tag="VQ620_001044"
     CDS             7..735
                     /locus_tag="VQ620_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2763681.1"
                     /translation="MAIVEIINLTKSFKDIEVIHNTSFYLNKGKVYGFVGPNGAGKTT
                     IIKMILGILKPDSGKITIFNQTVEQNSKNILSRIGLVLGPSFYGHLDAYKNLKLIANM
                     KGLSLDTERLNEYLSMVGLKDVKKKKVKNFSMGMKQRLSIAASLLGSPEILIWDEPIN
                     GLDPQGVIEIRSLIRFLQEKKGITFLISSHILSELDKVISDIIIINYGKVEFFGSCHY
                     LLQKYNCRNLEEAYLACLAGGEYD"
     gene            728..1465
                     /locus_tag="VQ620_001045"
     CDS             728..1465
                     /locus_tag="VQ620_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2763682.1"
                     /translation="MIKLEFLKQKKSILWFVLIFPIILNALLYIDLTFRYRGYLLVHQ
                     NELALSNWQLIFKEQTIFYFSELFYLVLSLIIYEVFAVEFKNDAWLTVISLPFRNKYT
                     INSKLLITVVYTFTFWLSDYISLYVIGKAIDNSLEIGLIFFLKTFTIQLISSLMIMLL
                     YFLTLVLIRKISGIIPIGIIMMILTISIYYNDYNFKIYLPFTYLSHAFRVTESQFYMI
                     LLSNIIIIVLFYILIRKLNERSFEMKL"
     gene            1478..2188
                     /locus_tag="VQ620_001046"
     CDS             1478..2188
                     /locus_tag="VQ620_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763683.1"
                     /translation="MKLLKNELIKSKIFLFIIVDICIQILVILAIKTYILDISAPYSE
                     LDYNKYWYVLHALIYMPMIFPIQILYQNLREALIEDNNNGWNIMVINTNNLVKIIYIK
                     VTINIVRCFICYFVYTIFSLIQLGGMGTDMLLTNIIFPNIMSFLLFLPIAIFMQICCI
                     RFDSILAKALPNILLILIVLITFQSDWNIFIPATYYYTEIQSTTNLGIKLLVCIWIMG
                     FEFSLLPKLIKLKEQNLV"
     gene            2731..2952
                     /locus_tag="VQ620_001047"
     CDS             2731..2952
                     /locus_tag="VQ620_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2763684.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            3469..3708
                     /locus_tag="VQ620_001048"
                     /pseudo
     CDS             3469..3708
                     /locus_tag="VQ620_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            3852..5081
                     /locus_tag="VQ620_001049"
     CDS             3852..5081
                     /locus_tag="VQ620_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET2763685.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            5101..7797
                     /gene="ppc"
                     /locus_tag="VQ620_001050"
     CDS             5101..7797
                     /gene="ppc"
                     /locus_tag="VQ620_001050"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET2763686.1"
                     /translation="MSLQKLENSSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATNLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYEIILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    7880..8097
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8210..9214
                     /locus_tag="VQ620_001051"
     CDS             8210..9214
                     /locus_tag="VQ620_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2763687.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     IELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            9283..10149
                     /locus_tag="VQ620_001052"
     CDS             9283..10149
                     /locus_tag="VQ620_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2763688.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            10146..10904
                     /locus_tag="VQ620_001053"
     CDS             10146..10904
                     /locus_tag="VQ620_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2763689.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            10994..12754
                     /gene="dnaG"
                     /locus_tag="VQ620_001054"
     CDS             10994..12754
                     /gene="dnaG"
                     /locus_tag="VQ620_001054"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET2763690.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            12757..13866
                     /gene="rpoD"
                     /locus_tag="VQ620_001055"
     CDS             12757..13866
                     /gene="rpoD"
                     /locus_tag="VQ620_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET2763691.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            13880..14209
                     /locus_tag="VQ620_001056"
     CDS             13880..14209
                     /locus_tag="VQ620_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET2763692.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            14324..15367
                     /gene="cpoA"
                     /locus_tag="VQ620_001057"
     CDS             14324..15367
                     /gene="cpoA"
                     /locus_tag="VQ620_001057"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HET2763693.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            15383..16708
                     /locus_tag="VQ620_001058"
     CDS             15383..16708
                     /locus_tag="VQ620_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2763694.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGILIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGALYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFRTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            16739..16915
                     /locus_tag="VQ620_001059"
     CDS             16739..16915
                     /locus_tag="VQ620_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET2763695.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            16974..17108
                     /locus_tag="VQ620_001060"
     CDS             16974..17108
                     /locus_tag="VQ620_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HET2763696.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            17172..18482
                     /gene="obgE"
                     /locus_tag="VQ620_001061"
     CDS             17172..18482
                     /gene="obgE"
                     /locus_tag="VQ620_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET2763697.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            18492..18650
                     /locus_tag="VQ620_001062"
     CDS             18492..18650
                     /locus_tag="VQ620_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763698.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            19700..20959
                     /locus_tag="VQ620_001063"
     CDS             19700..20959
                     /locus_tag="VQ620_001063"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET2763699.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            20975..21526
                     /locus_tag="VQ620_001064"
     CDS             20975..21526
                     /locus_tag="VQ620_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2763700.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            21519..22796
                     /locus_tag="VQ620_001065"
     CDS             21519..22796
                     /locus_tag="VQ620_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2763701.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIDRLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            22812..23672
                     /locus_tag="VQ620_001066"
     CDS             22812..23672
                     /locus_tag="VQ620_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HET2763702.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            23895..24005
                     /locus_tag="VQ620_001067"
                     /pseudo
     CDS             23895..24005
                     /locus_tag="VQ620_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase"
BASE COUNT         7491 a         4008 c         5215 g         7404 t
ORIGIN      
        1 gtagagatgg ctatagttga aattataaat ctaacaaaaa gctttaaaga tattgaagtt
       61 attcataaca cttcatttta tttaaataag ggaaaagtat atggttttgt tggaccaaat
      121 ggggctggaa aaactacaat tatcaaaatg attctaggga ttttaaaacc agattctggg
      181 aaaataacta tttttaatca aaccgtagaa cagaattcca aaaatatatt atctaggata
      241 ggattagttt tgggaccttc attttatggt catcttgatg cttataaaaa tttgaagtta
      301 atagcaaata tgaaagggct tagtttagat actgaaagac ttaatgaata tctatcaatg
      361 gtaggattaa aagatgttaa aaagaaaaaa gtgaaaaatt tttcaatggg catgaaacaa
      421 cgtctaagta tagctgcatc attgttaggt agtccagaaa tattaatttg ggatgaacca
      481 atcaatggac ttgatccaca gggtgtaatt gaaattagaa gtttaatccg ttttctacag
      541 gagaaaaaag gtattacatt tttaatatca agtcatattc ttagtgaact ggataaagtg
      601 atatctgata ttataattat aaattatggg aaagttgaat tttttggttc ttgccactac
      661 cttttacaaa agtataattg tcgaaatctg gaagaagcct acttagcttg tttagctgga
      721 ggtgaatatg attaaattag agtttctaaa gcagaaaaaa agcatcttat ggtttgttct
      781 tatttttcca ataattctga atgctttgtt atatatagac ttaactttta ggtatcgagg
      841 ttatttattg gtgcatcaaa atgaacttgc tttatctaat tggcagttaa tttttaaaga
      901 acaaacaata ttttattttt ctgaactatt ttatttagtt ctatctttaa taatttacga
      961 agtgtttgca gtagaattta aaaatgatgc atggttaacc gtaatttcat tgccatttag
     1021 aaacaagtac acaataaatt ctaaattact cattacagtg gtttacacat ttactttttg
     1081 gttgtctgat tatattagct tatacgttat tgggaaggct attgataaca gtttagagat
     1141 tggtttgatt ttctttttaa aaacatttac cattcaatta atttcctcgt tgatgataat
     1201 gttattatat tttctaactt tagtacttat aaggaaaatt tctggaataa taccaattgg
     1261 tattataatg atgattttaa caattagcat atactacaac gattataatt ttaagattta
     1321 cttaccattt acttatcttt ctcatgcttt tagagtgact gaaagtcaat tttatatgat
     1381 tttactatct aatataataa tcatcgtact tttttatata ttaattcgta aattgaacga
     1441 aagaagtttt gagatgaaac tttaataggt aataaaaatg aagttactta aaaatgaatt
     1501 gattaagagt aaaatatttt tatttattat tgttgacatc tgtattcaga ttctagtcat
     1561 tctagctata aaaacatata ttttagatat ttcggcacca tactcagagc ttgattataa
     1621 taagtattgg tatgttttac acgctttaat atatatgcca atgatttttc ctatacaaat
     1681 tttgtatcaa aatctaagag aggcattgat tgaagataat aataatggtt ggaatatcat
     1741 ggtgattaac actaataatc tcgtgaaaat catctatata aaagtaacga taaatatcgt
     1801 gagatgtttt atttgttatt ttgtttatac aattttttct ttaattcaat taggtggtat
     1861 gggaactgat atgttattaa ccaatataat atttcccaat ataatgtcat ttctgttatt
     1921 tttacctata gctatcttta tgcaaatttg ctgtataaga tttgattcca ttttagcaaa
     1981 ggccttacct aatattttgc tgatacttat tgttttaatc acgtttcaga gtgattggaa
     2041 tatatttata cctgcaactt attattatac agaaattcag tcaactacaa acttaggtat
     2101 taaattatta gtttgtatct ggattatggg atttgaattt tcccttcttc caaaactaat
     2161 aaaattgaaa gaacaaaatc tagtttaaat gttgctcgaa aattaaataa ttgaatacga
     2221 ttaacaatga aacaatacaa cgtgagccat gacttcaaag taattgattt tatgattctg
     2281 gtttcttacg cttaattaag caagttgtat tcactttatc aaattgttat tactctaaaa
     2341 agcgtctaac agctatagag tgtctgtttg cttctcttta cttttatttt aaataattca
     2401 ttaaccttcc gtaagtgccc aataataagg tgtgttgaag aagtctgaga taaaatagtt
     2461 ctcagtcaca aaaaaagcta tagattccca ttggtgaaca gctccctgtc aagtagacag
     2521 taaaataata taaaatctta agcgacctga ttcctgaatt ccagtggagt caggttgttt
     2581 tttaaccgtc tttcttaata ttgtctcacc tataatagat atgcttatga gatatgttta
     2641 ataaattaat tttagttcaa aatgtccagt taggacactt tgaatgattt cttagtttta
     2701 tatattatac ttttctcagg agaaagctag atgtacagac gtttgagaga tttgagggaa
     2761 gatcacgatt tgacccaaaa acaaatagct aaaatacttt cgtttacaaa ctcagcttat
     2821 gctaaaattg aacggggtga gcatacgtta acagcagatg tattggtaac tctctcaaac
     2881 ttttacgatg tcagtacaga ctacctattg gaattgacag attttcctga taaaattcgc
     2941 tttagaaaat aatctcctca atttcataga gtgtgaaaat gaggagattt tttatttgtt
     3001 ctttgaaaat taaatagtcc aaaatggtac tttcctcatt tgtggagcaa atttgaatgg
     3061 ctcgccatga taagagcgat tttaaagcca tcaataaaat agagcgatac tttatatgcc
     3121 atgatacaaa tgatatacaa tgatacttct gaccgttcag gctgccatcg taaaagagca
     3181 gcaagtgaaa ttcttatgat gacttcatca gtcatgccac gttgaatgtg tgagtttgtt
     3241 agagaaacgc aattaatcct caaaaggttc cccgaacctt ttgagttcca cagacgcatc
     3301 acgtggagtg tgtaagcttg ttgctaaaag cgtaaaaacc ttggaacgaa aggaataata
     3361 gacttcctgc gaaacaaaaa tacaatacaa taaaactatg gatgatgaag caagtaaaca
     3421 attaagcgat agccgtttca agagccttgt aggtgttcag cgcacgagat gttagctgtg
     3481 ttaaaaacag cttatcaacg caacgtaaac gcgcaaaagg tggacgaaaa accaaattaa
     3541 gcctagacga tctcctcatg gtaactattc aatacatgcg agaatagcgc acttatgaac
     3601 aaattgcggc tgattttggc attcacgaaa gcaacataat tagtcggagg tcaatggatt
     3661 gaagcaactc ttattcaaaa tggttttacg atttcaaatt ctgccttaat tctgtaaaaa
     3721 cagtaaaatt cgaaggattg taaggtaaga gtttttttct ttctgaaaaa atggtataat
     3781 agcaatcaaa actagaaaat aaaacggaat ttggaacaga tttgtctgta tcctagtaga
     3841 gtggtgatac tatgaagatt agtaagaggc acttattaaa ttattccatc ttgattccct
     3901 acttgctttt atctattttg ggcttgattg tggtctattc gaccaccagt gctattttaa
     3961 ttgaagaagg caagagcgcc ttgcagttgg ttcgaaacca aggaatcttt tggattgtta
     4021 gtttgatact gattgcctta atttataaat tgagactaga ttttttgaga aatgagcgac
     4081 taatcatttt agttatatta atagaaatgc ttttattgtt cttggctcgt tttattggta
     4141 tttcagtaaa cggggcatac ggttggattt cggttgcagg agtaactatt cagccagctg
     4201 agtacttaaa aatcattatt atttggtatt tagctcaccg attctccaaa cagcaagaag
     4261 aaatagctac ttatgatttt caagttttga ctcaaaatca atggcttccc cgtgctttta
     4321 atgattggcg attcgttctc ctagttctga ttggaagttt gggaattttc cctgatttag
     4381 gaaatgcgac tattttagtc ttggtttcct tgattatgta tacagttagt ggaatcgctt
     4441 atcgctggtt ttcaaccatt ctggcgctcg tatctgccgc ttctgtcttt gtcttgacca
     4501 ctatcagcct aatcggtgtt gagacctttt caaaaattcc agtatttggc tatgtagcca
     4561 aacgctttag tgcctttttt aatccttttg ccgatcgtgc tgatgcaggt caccagttag
     4621 ctaattctta ttttgccatg gtcaatggtg gttggtttgg tctaggtctt ggaaactcga
     4681 ttgaaaaacg aggttatttg ccagaagctc atacagactt tgtcttttct atcgtgattg
     4741 aagaatttgg ctttgttggt gccagtctta ttttagctct cttgtttttc atgattttgc
     4801 ggattatctt ggtcggtatt cgagcggaga atcctttcaa tgccatggtt gcactcggtg
     4861 tcggagggat gatgttggtt caggtatttg tcaatatcgg agggatttcg ggcttgattc
     4921 catctacagg agtaaccttc cccttcttat cccagggtgg aaatagtctt ctagtcttat
     4981 cagtggcagt agcctttgtc ttaaatattg atgccagtga aaaacgcgct aagttgtacc
     5041 gagaattgga aaatcaacca atgaaccttc tgttgaagta ggataaagaa aggatagttt
     5101 atgtctcttc aaaaattaga aaattctagt aataaaagtg ttgtgcaaga agaagtcttg
     5161 attctaacag aattactgga agatattact aaaaatatgc ttgccccaga gacctttgaa
     5221 aaaataatac agttgaaaga attatcaacg caggaagatt atcaaggtct aaaccgtcta
     5281 gtgactagct tatcaaatga tgaaatggtc tatatttcac gctatttctc tatcttgcct
     5341 cttttgatta atatttcaga ggatgtggat ttagcttatg aaatcaatca tcaaaataat
     5401 attgatcagg actatttagg taaattatct acaacgatta aattggtagc agaaaaggaa
     5461 aatgccgttg agatcctaga acacttgaat gttgttccgg ttttgacagc ccatccaaca
     5521 caagtgcaac gcaaaagtat gttggattta acaaatcata ttcatagtct tttgcgtaaa
     5581 taccgtgatg ttaagttggg gttgatcaat aaagataaat ggtacaatga tttgcgtcgt
     5641 tacatcgaaa ttatcatgca gacagacatg attcgtgaga aaaaattaaa agtgactaac
     5701 gaaatcacga atgctatgga atattataac agctcctttt tgaaagctgt acctcatttg
     5761 acgacggagt ataagcgctt agcgcaagcg catggtctga atttaaaaca ggctaaacca
     5821 atcaccatgg gtatgtggat aggtggtgac cgtgatggaa atccatttgt tacagcaaag
     5881 accttgaagc agtctgcact cactcaatgt gaagtcatca tgaactacta tgataaaaag
     5941 atttaccaac tttatcgtga attttctctt tcaactagca ttgtcaacgt cagcaagcaa
     6001 gtcagagaaa tggctcgtca atccaaggat aactcgattt accgcgaaaa agagctttac
     6061 cgtcgtgcct tgtttgatat tcaatcaaaa attcaggcaa ctaaaaccta tctgattgag
     6121 gatgaagaag ttgggactcg ttatgaaacc gccaatgatt tctacaagga tttgattgcc
     6181 attcgagatt ctctactaga gaataagggc gagtccttga tttcaggtga ttttgtggaa
     6241 ttattgcagg cagtagagat atttggtttt tacctagcat caattgatat gcgacaagac
     6301 tctagcgtct atgaagcctg tgtggcagaa ctcttgaaat cagcaggaat tcattctcgt
     6361 tatagcgagt tgagcgaaga agaaaagtgt gaccttcttt tgaaagaatt agaagaagat
     6421 ccccgaattc tttctgcgac tcacgcagaa aaatcagaat tattagcaaa agaactagct
     6481 atttttaaga cggctcgtgt tttgaaagat aagttgggag atgatgtcat ccgtcagacc
     6541 atcatttcac atgcaaccaa cctttctgat atgctagaat tagctattct gttaaaagaa
     6601 gtaggactgg tggatacgga aagggcgcgt gttcagattg ttcccctttt tgaaacaatt
     6661 gaagacttgg atcattcaga ggaaacaatg agaaaatatc tttctcttag ccttgccaaa
     6721 aaatggattg actcacgaaa taactaccaa gaaatcatgc ttggctactc tgacagtaat
     6781 aaagatggcg gttacttgtc atcatgttgg accctctaca aggctcaaca acaattgact
     6841 gctattggag atgaatttgg cgttaaggtt accttcttcc atggtcgtgg tggtactgtc
     6901 ggtcgtggtg gtgggccaac ctatgaagcc attacatctc aaccgctcaa gtctatcaag
     6961 gatcgtatcc gtttgacgga gcagggtgaa gtaattggga ataaatacgg taacaaagac
     7021 gccgcttact ataaccttga aatgctagta tcggcagcta ttaaccgtat gattactcag
     7081 aagaagagcg ataccaatac cccaaatcgt tatgaagcca ttatggatca agtagtggac
     7141 cgtagttacg atatctaccg tgatttggtc tttggtaatg agcatttcta tgattatttc
     7201 ttcgagtcaa gtccaatcaa ggctatttca agttttaata ttggttctcg tccagccgct
     7261 cgtaagacta ttactgaaat cggtggtttg cgtgccatcc cttgggtatt ctcatggtca
     7321 cagagtcgtg ttatgttccc tggatggtac ggggttggtt caagcttcaa ggaatttatc
     7381 aataaaaatc cagagaatat tgctatctta cgagatatgt accaaaattg gcctttcttc
     7441 caatcgcttc tttcaaatgt tgatatggtt ttgtcaaaat caaatatgaa tattgctttt
     7501 gaatatgcta aactttgtga agacgagcaa gttaaggcca tctatgagat tattttaaat
     7561 gaatggcaag ttactaagaa cgttatcttg gctattgaag gacatgacga actcttagct
     7621 gacaatccat atctaaaagc tagtctggat taccgtatgc cttactttaa tattctcaac
     7681 tatattcagt tagagttgat taaacgccaa cgtcgtggag aattgtccag tgatcaagaa
     7741 cgattgattc atatcaccat caacggaatt gcgacaggat tgcgtaattc aggttgataa
     7801 ttttcaagag tgaatgctaa aagtgaatat caaaaaaatt ctaatagact attgacaagt
     7861 agtttaaaaa tgatataatt taaccattca gaaaagtaat catacaaact ttttagagag
     7921 tctgtggtag ctgaaaacag ataagtggcg atgatgaaaa ttgggctgaa tgctatttag
     7981 aatttgaaat tataaaaatt cggtaagcac accttacagt gcatctcgtt attgcgagac
     8041 tgagcgatag ggaaattccc tataattgag gtggtaccgc gcatcgacgt cctcacacaa
     8101 gttttttgtg tgaggatttt tttgatggag gttagtatgg aaagaaaacg atggcgtcgc
     8161 ttgtttagat aagtgaaata tgttaaagga aataaaaagg agaaacagaa tgaaaaataa
     8221 acgtttaatt ggaattattg ctgcattagc agtcttagta gcaggaagct tgatttattc
     8281 ttcaatgaat aaatcagaag ctcagaataa taaggatgag aagaaaataa ccaagattgg
     8341 tgtgcttcaa tttgtgagcc atccatccct tgatttgatt tataaaggga tccaagatgg
     8401 acttgcagaa gaaggatata aagatgatca agttaaaatt gattttatga actcagaagg
     8461 tgatcaaagt aaggttgcga caatgagtaa acaattggtt gcaaatggga atgaccttgt
     8521 ggttggtatc gcaacaccag cagcccaagg gttggctagt gcaacaaaag acctaccagt
     8581 tatcatggcc gctattacag acccaattgg tgctaacttg gttaaagatt tgaaaaaacc
     8641 aggtggtaac gttacagggg tatctgacca caatccagct caacaacaaa ttgaactcat
     8701 caaggctctg acaccgaatg tgaaaacaat cggagctctt tactcaagta gcgaagacaa
     8761 ttcaaaaaca caggttgaag aatttaaggc ttatgctgaa aaagcaggtc tgacagtgga
     8821 aacatttgca gttccttcaa caaatgaaat tgcctcaact gtcactgtta tgactagcaa
     8881 ggtagatgct atttgggttc caattgataa caccattgca tcaggatttc caacggttgt
     8941 ctctagcaat caaagttcta agaaaccaat ttatcccagt gcgacagcta tggtagaagt
     9001 aggtggtttg gcatcagttg taattgacca acatgacctt ggtgtggcaa caggtaaaat
     9061 gattgtgcaa gtcttgaaag gtgcaaaacc agccgatacc ccagtcaatg tcttttcaac
     9121 tggtaagtca gtcatcaata aaaaaatagc acaagaacta ggtattacta ttcctgagtc
     9181 tgttctcaaa gaagcaggac aagtcatcga ataaataagg tagggaggag gagactcttc
     9241 ccatttttta aaaagatgaa tatagaaagg attaaataaa atatgattgt ttccattatt
     9301 tctcaaggat ttgtctgggc tattctaggt ctgggaatct ttatgacatt taggatttta
     9361 aactttccag atatgacgac agaaggttcc ttccctcttg ggggagctgt tgctgtcact
     9421 ttgataacca aaggcgtgaa cccattttta gcgacacttg ttgctgtagg agcaggttgt
     9481 ttggctggaa tggcagcagg ccttctttat acaaaaggga agatcccaac cttgctctca
     9541 gggattttgg tgatgacttc ttgtcactca atcatgctct tgattatggg acgtgcgaat
     9601 ttaggcctgc ttggaaccaa gcaaattcag gatgttttgc cttttgattc ggatttgaat
     9661 caactcttga caggtctcat ctttgtgagc attgttattg ctctcatgct ctttttcttg
     9721 gacactaaac tcggacaagc ctatattgct acaggggata atcctgatat ggctagaagt
     9781 ttcgggattc atactggacg catggagctc atgggcttgg tcttatcaaa tggtgtgatt
     9841 gcccttgcag gtgccctcat tgctcagcaa gaaggctatg ccgatgtgtc tcgagggatc
     9901 ggggttatcg ttgtggggct tgcaagtttg attattggag aagttatttt caagagtttg
     9961 agcttggcag agcgtttggt tactatcgtt gtaggttcta tcgcttatca atttttagtg
    10021 tgggcagtta tcgcacttgg ctttaataca agttaccttc gtttatacag tgccttgatt
    10081 ttagcagtct gcctcatgat tccaacattt aagcaaacaa tcttgaaagg agccaagtta
    10141 agcaaatgac agcaattgta gaattaaaaa atgcaaccaa aattgttaaa aatggctttg
    10201 atgaagaaaa gattatttta aatgatgttt ccttagaaat ttttgaacgg gactttatca
    10261 cgattttggg cggaaatggt gctggaaaat caaccctctt taacactata gcagggacct
    10321 tatcactaac tagtggaact atccgtattt taggtgaaga tctcactaag ttttcacccg
    10381 agaagcgtgc caagtacctg tctcgtgtct tccaagatcc aaagatgggg acagctcccc
    10441 gtatgacggt cgctgaaaat cttttaatcg ccaagtttcg tggtgagaag cgtggattgt
    10501 taccacgacg cttgactagc tataaggatg aatttcaggc aaccattgaa aaagtaggaa
    10561 atggtcttga gaaacacttg aatacaccga ttgagttctt atcaggtgga caaagacagg
    10621 ctttgagtct cttgatggca accttgaagc gacctgaatt actcctgtta gatgagcata
    10681 ctgctgccct ggatccaaag actagtgttg ctttgatgga attgacagat gaatttgtta
    10741 agaaagatca gctaacagcc cttatgatta ctcatcatat ggaagatgct ctcaaatacg
    10801 gcaatcgctt gattgtcatg aaagaaggac ggattatcca agatttaaac caagaagaaa
    10861 aagcaaaaat gaaaatctct gattattatc aactctttga ataaagtagg gaaaatgagt
    10921 gaaatggttt actttttttc tgaaataaag tatactatat aaagtaaact atgataacat
    10981 ggaggtattg tgtatggttg acaaacaagt cattgaagaa atcaaaaaca atgccaacat
    11041 tgtggaagtc ataggagatg tgatttcttt acaaaaggca ggacggaact atctagggct
    11101 ctgtcctttt catggtgaaa aaacaccttc tttcaacgtt gtagaggaca agcagtttta
    11161 ccactgtttt ggttgtggtc gctcaggtga tgtctttaaa ttcatcgagg agtaccaagg
    11221 ggttaccttt atggaggctg tccaaatctt aggtcagcgt gtcgggattg aggttgaaaa
    11281 accgctttat agtgaacaga agccagcctc gcctcaccaa gctctttatg atatgcacga
    11341 agatgcggct aaattttacc atgctattct catgacaacg actatgggcg aagaggccag
    11401 aaattacctt tatcagcggg gtttgacaga tgaagtgctt aaacattttt ggattggttt
    11461 agcacctcca gaacgaaact atctctatca acgtttgtct gatcagtatc gtgaagagga
    11521 tttactggat tcaggcctgt tttatctctc ggatgccaat caatttgtag acacctttca
    11581 caatcgcatt atgtttcccc tgacaaatga ccaaggaaag gtcattgcct tctcaggtcg
    11641 tatctggcaa aaaacggatt cacaaacttc taagtataaa aacagccgat cgactgcaat
    11701 ttttaacaaa agttacgaat tatatcatat ggatagggca aaaaaatctt ctggaaaaac
    11761 tagtgagatt tacctgatgg aaggattcat ggatgttatt gcagcctatc gggctggaat
    11821 cgaaaatgct gtggcgtcga tgggaacggc cttgagtcga gagcatgttg agcatctgaa
    11881 aaggttaacc aagaaattgg ttcttgttta cgatggagat aaggctgggc aagccgcgat
    11941 attgaaagca ttggatgaaa ttggtgatat gcctgtgcaa atcgttagca tgcctgataa
    12001 cttggatcct gatgagtatc tacaaaaaaa tggtccagaa gacttggcct atctattaac
    12061 aaaaactcgt attagtccga ttgagttcta cattcatcag tacaaacctg aaaacagtga
    12121 aaatctgcag gctcagattg agtttattga aaaaatagct cccttgattg ttcaagaaaa
    12181 gtccatcgct gctcaaaaca gctatattca tattttagct gacagtctgg cgtcctttga
    12241 ttatacccag attgagcaga ttgttaatga gagtcgtcag gtgcaaaggc agaatcgcat
    12301 ggaaagaatt tccagaccga cgccaatcac catgcctgtc accaagcagt tatcggctat
    12361 tatgagggca gaagcccatc tactctatcg gatgatggaa tcccctcttg ttttgaacga
    12421 ttaccgtttg cgagaagact ttgcatttgc tacacctgaa tttcaggtct tatatgactt
    12481 gcttggccag tatggaaatc ttcctccaga agttttagca gagcagacag aggaagttga
    12541 aagagcttgg taccaagttt tagctcagga tttgcctgct gagatatcgc cgcaggaact
    12601 tagtgaagta gagatgactc gaaacaaggc tctcttgaat caggacaata tgagaatcaa
    12661 aaagaaggtg caggaagcta gccatgtagg agatacagat acagccctag aagaattgga
    12721 acgtttaatt tcccaaaaga gaagaatgga gtaataatgg caacaaaaca aaaagaagta
    12781 acaacatttg acgtacaggt agcagaattt atccgtaatc ataagcaaaa agggacagca
    12841 acagatgatg aaatcaatgc aagtctggtt attcctttta ccttggacgc tgatgggatt
    12901 gaagatctct tgcaacggat tcaggatgca gggatttcta tcacagataa cgaaggaaat
    12961 ccaagtgcgc gtgttcttag caatgaagaa gaaccagaac tcagcgatga ggacttgatt
    13021 ggatcaactt ctgctaaggt caatgaccct gtccgtatgt acttgaaaga aataggggtc
    13081 gttcctctct tgaccaatga agaggagaaa gagttggcac tggctgttga agctggcgat
    13141 atcgaagcca aacaacgtct tgcggaagcc aatcttcgtt tggttgtttc cattgccaaa
    13201 cgctatgtcg gtcgtggcat gcagttcctt gacttgattc aagaaggaaa tatgggcttg
    13261 atgaaggcgg ttgacaagtt tgactattct aaagggttca agttttcaac ttatgcaact
    13321 tggtggattc gtcaggctat cactcgtgct attgcggacc aagctcgtac catccgtatc
    13381 ccagttcaca tggttgaaac tatcaataaa ttggttcgtg aacagcggaa tctccttcaa
    13441 gaattggggc aagatccgac accagaacag attgctgaac gaatggatat gacacctgat
    13501 aaggttcgtg aaatcttgaa gattgcccaa gaaccagtat ctcttgaaac tcctatcggt
    13561 gaagaggacg atagccacct tggagacttt atcgaagatg aagtgattga aaatccagtg
    13621 gattatacga ctcgtatcgt cttgcgtgag caattggatg aaatcttaga tactcttaca
    13681 gaccgtgaag aaaatgttct gcgtctacgt tttggactag atgatggaaa aatgcgcaca
    13741 cttgaagatg tggggaaagt ctttaacgta actcgtgagc gtatccgtca gattgaagca
    13801 aaggctttga gaaaactacg ccaaccaagt cgcagcaaac cgcttcgtga ttttattgaa
    13861 gactaagagt gaggaaaata tggcttatac agaagagcaa attgaaaaca tcaaaacacg
    13921 gattttaaca gccttggaag aagtcatcga ccctgagttg ggaatcgata ttgtcaatct
    13981 tggtttgatc tatgagattc gttttgacgg tgacacaggg caaacagaga ttgacatgac
    14041 tttgacaact atgggttgtc ccttagcaga ccttttgaca gaccagattt atgatgctat
    14101 gatagaggtt ccagaagtaa cggatactga ggttaaatta gtctggtatc cagcctggac
    14161 agttgaaaaa atgagtcgct atgcccgcat tgccctaggc attaagtaaa catataaaag
    14221 catgtgagag actagaggaa aagcgaggaa atttcccctc ttttcctcta gtctctcctt
    14281 tcttttgctg attttattca aagaaaatga tataatagta gttatggaga aaaagaaatt
    14341 acgcatcaat atgttgagtt caagtgagaa agtagcagga cagggagttt caggtgctta
    14401 ccgtgaatta gttcgtcttc ttcaccgtgc tgccaaggac caattgattg ttacagaaaa
    14461 tcttccaatc gaggcagatg tgactcactt tcatacgatt gattttccct attatttatc
    14521 aaccttccaa aagaaacgct cagggagaaa gattggctat gtgcatttct tgccagctac
    14581 acttgaagga agtttgaaaa ttccattttt cttaaaggga attgtgaaac gctatgtatt
    14641 ttctttttac aaccggatgg agcacttggt tgtggtcaat cctatgttta ttgaggattt
    14701 ggtagcagct ggtattccac gtgaaaaagt gacctatatt cctaactttg tcaacaagga
    14761 aaaatggcat cctctaccac aagaagaggt agtcagactg cgcacagatc ttggtcttag
    14821 tgacaatcag tttatcgtag taggtgctgg gcaagttcag aaacgtaaag ggattgatga
    14881 ctttatccgt ctggctgagg aattgcctca gattaccttt atctgggctg gtggcttctc
    14941 ttttggtggt atgacagatg gttatgaaca ctataagaaa attatggaaa atccccctaa
    15001 aaatttgatt tttccaggca ttgtatcgcc agagcggatg cgcgaattgt atgctctagc
    15061 ggatcttttc ttgttgccta gttacaatga gctctttcct atgactattt tagaagctgc
    15121 gagttgtgag gctcctatta tgttgcgtga tttagatctc tataaggtga ttttggaggg
    15181 aaattatcgg gcgacagcgg gtagagaaga gatgaaagag gctattttgg aatatcaagc
    15241 aaatcctgct gtcttaaaag atctcaaaga aaaggctaag aatatttcca gagagtattc
    15301 tgaagagcat ctgttacaaa tctggttgga cttttatgag aaacaagccg ctttagggag
    15361 aaagtaaaaa gtgaggtaat ctatgcgaat tggtttattt acagatacct attttcctca
    15421 ggtttctggt gttgcgacca gtattcgaac cttgaaaaca gaacttgaaa agcagggaca
    15481 tgctgttttt atctttacga cgacagataa ggatgtcaat cgctacgaag attggcaaat
    15541 tatccgcatt ccaagtgttc ctttctttgc ttttaaggat cgtcgctttg cctaccgagg
    15601 ttttagcaag gcacttgaaa ttgctaaaca gtatcagcta gatattatcc atactcagac
    15661 agaattttct cttggcctgt tggggatttg gattgcgcgt gaattgaaaa ttccagtcat
    15721 ccatacctat cacacccagt atgaagacta tgtccattat attgctaagg ggatattgat
    15781 ccggccgagt atggtcaagt atctggttag aggtttcctg catgatgtgg atggggttat
    15841 ttgccctagt gagattgtcc gtgacttgct atctgattat aaggtcaagg ttgaaaaacg
    15901 ggtcattcct actgggattg aattagccaa gtttgagcgt ccggaaatca agcaggaaaa
    15961 tttgaaagaa ctgcgtagta aactagggat tcaagatggt gaaaagacgt tgcttagtct
    16021 ttcgagaatc tcctatgaaa aaaatattca agcagtttta gcagcctttg ctgatgttct
    16081 gaaagaggaa gacaaggtta aactggtagt agctggggat ggcccttatc tgaatgacct
    16141 caaagagcaa gcccagaacc tagagattca agactcagtc atctttacag ggatgattgc
    16201 tcctagtgag acggctcttt actataaagc ggcggatttc ttcatttcgg catcgacaag
    16261 cgaaacgcaa ggtttgacct acttggaaag cttagccagt ggaacacctg tcattgctca
    16321 cggaaatcct tatttgaaca acctcatcag tgataaaatg tttggagcct tgtactatgg
    16381 agaacatgat ttggctggtg ctattttgga agccctgatt gcaacaccag acatgaatga
    16441 gcatacctta tcagagaaat tgtatgagat ttcagctgag aactttggga aacgagtgca
    16501 tgagttttat ctggatgcca ttatttcaaa taacttccag aaagatttgg ctaaagatga
    16561 tacggtcagt cagcgtatct ttaggacagt tttgtatctt ccgcaacagg tggttgctgt
    16621 acctgtaaaa ggatctagac gcatgttgaa ggcttcaaaa acacagttga tcagtatgag
    16681 agactattgg aaagaccatg aagaatagaa agaggaacag ctatgaaaaa aacaattaat
    16741 gagaagcggt cgtgataaaa agattgcggg tgtttgtgct ggggtggccc attatctgga
    16801 tatggatccg actatcgttc aagtcatttg gggtgttctt acttgctgtt acggagctgg
    16861 aattgtagct tacattattt tatggattat cgcaccagta gcaactgact attgaaaact
    16921 agctcaattc atgctaagat aatagaaaat agaatgtaac gaaggagaga aaaatggcat
    16981 ttggagataa tggaaatcgt aaaaaaacta tgtttgagaa aataaccttg tttatcgtga
    17041 ttatcatgct agtagcaagt ttattgggaa tttttgcaac tgcaattggt gccctcagta
    17101 atctataaaa tagattcaag aaaatttagt gactgggatt tcccagccct tttttaaagt
    17161 gagaagaaat aatgagtatg tttttagata cagctaagat taaggtcaag gctggtaatg
    17221 gtggcgatgg tatggttgcc tttcgtcgtg aaaaatatgt ccctaatgga ggcccttggg
    17281 gtggtgatgg tggtcgtgga ggcaatgtgg tcttcgttgt agacgaagga ctacgtacct
    17341 tgatggattt ccgctacaat cgtcatttca aggctgattc tggtgaaaaa gggatgacca
    17401 aagggatgca tggtcgtggt gctgaggacc ttagagttcg agtaccacaa ggtacgactg
    17461 ttcgtgatgc ggagactggc aaggttttaa cagatttgat tgaacatggg caagaattta
    17521 tcgttgccca cggtggtcgt ggtggacgtg gaaatattcg tttcgcgaca ccaaaaaatc
    17581 ctgcaccgga aatctctgaa aatggagaac caggtcagga acgtgagtta caattggaac
    17641 taaaaatctt ggcagatgtc ggtttagtag gattcccatc tgtagggaag tcaacacttt
    17701 taagtgttat tacctcagct aagcctaaaa ttggtgccta ccactttacc actattgtac
    17761 caaatttagg tatggttcgc acccaatcag gtgaatcctt tgcagtagcc gacttgccag
    17821 gtttgattga aggggctagt caaggtgttg gtttgggaac tcagtttctc cgtcacatcg
    17881 agcgtacacg tgttatcctt cacatcattg atatgtcagc tagcgaaggc cgtgatccat
    17941 atgaggatta cctagctatc aataaagagc tggagtctta caatcttcgc ctcatggagc
    18001 gtccacagat tattgtagct aataagatgg acatgcctga gagtcaggaa aatcttgaag
    18061 aatttaagaa aaaattgtct gaaaattatg atgaatttga agagttacca gctatcttcc
    18121 caatttctgg attgaccaag caaggtctgg caacactttt agatgctaca gctgaattgt
    18181 tagacaagac accagaattt ttgctctacg acgagtccga tatggaagaa gaagcttact
    18241 atggatttga cgaagaagaa aaagcctttg aaattagtcg tgatgacgat gcgacatggg
    18301 tactttctgg tgaaaaactc atgaaactct ttaatatgac caactttgat cgtgatgaat
    18361 ctgtcatgaa atttgcccgt cagcttcgtg gtatgggggt tgatgaagcc cttcgtgcgc
    18421 gtggagctaa agatggggat ttggtccgca ttggtaaatt tgagtttgaa tttgtagact
    18481 aggagactgg tatgggagat aaaccgatat ctttccgaga tgcggatggt aattttgttt
    18541 ccgccgcaga cgtttggaat gaaaagaaat tggaagaact atttaatcgt ctcaatccaa
    18601 atcgtgcctt gagattggca cgaactaaaa aggaaaatcc atctcagtaa agaagctaaa
    18661 aaatcccgtg cctcatcaga cacgggattt tgtggtacga caggcatgta tagcaaactg
    18721 aatctggaat agcacagcat atcttctaaa atattgttat aaattgattt gaatttcaaa
    18781 attaaattgt ttgattctta tctcaatttg ttatagtata tctgatgtca aagttctcgg
    18841 cgagtcaaat agcgattccc aagcctgact atcgtgaggt agcggattaa aatggtctgg
    18901 ggatagaccg ttttaagtct gacgctggaa ataagaattg tcagaagaag ggatagcgaa
    18961 atcgtggctc tacgaacagg aacgtgataa taaggcgtat atagcggata agagggcctc
    19021 aaactctaaa gtccaaaaag gtagtcgtaa cctatatgcg taaatcacga gagtaattga
    19081 attcgtacta agattttcta ttttcactgt aaccttttaa cgtccttata tcttgtatac
    19141 acgaggaaag atgtacgact tatcccgtga ggtctatcac tataaagaga aaacgacaga
    19201 tagaagtgat cctgagtcac ggttatcttt aaaatagatt gtctgatagg acggtatgta
    19261 taaaacgctt ctgtgaactg agagaagggg gagaagttct tgctaaaatt tagttgaaca
    19321 gccgtattcc gatacttaga taagagatct agtcttagct cctactcagt tttaggggat
    19381 aaaaaagggg aaatagcgat tcgagaaaga ttatactctt cgaaaatctc ttcaaatcac
    19441 gtcaatatcg ccttgccgta tgtgtaggat actgactacg tcagttccat ctacaacctc
    19501 aaaacagtgt tttgagcaac ctgcggctag tttcctagtt tgatctttga ttttcattga
    19561 gtattagtaa ttcagttact aactcgtcaa ctctgattta tccaataaaa ttgaaaagga
    19621 tggaaaaaag gataaattta tgatatactt tattttgaag accttattag aaatcttgaa
    19681 agagtattga aaacttagaa tgagaaaaat tgttatcaat ggtggattac cactgcaagg
    19741 tgaaatcact attagtggtg ctaaaaatag tgtcgttgcc ttaattccag ctattatctt
    19801 ggctgatgat gtggtgactt tggattgcgt tccagatatt tcggatgtag ccagtcttgt
    19861 cgaaatcatg gaattgatgg gagctactgt taagcgttat gacgatgtat tggagattga
    19921 cccaagaggt gttcaaaata ttccaatgcc ttatggtaaa attaacagtc ttcgtgcatc
    19981 ttactatttt tatgggagcc tcttaggccg ttttggtgaa gcgacagttg gtctaccggg
    20041 aggatgtgat cttggtcctc gtccgattga cttacacctt aaggcgtttg aagctatggg
    20101 tgccactgct agctacgagg gagataacat gaagttatct gctaaagata caggacttca
    20161 tggtgcaagt atttacatgg atacggttag tgtgggagca acgattaata cgatgattgc
    20221 tgcggttaaa gcaaatggtc gtactattat tgaaaatgca gcccgtgaac ctgagattat
    20281 tgatgtagct actctcttga ataatatggg tgcccatatc cgtggggcag gaactaatat
    20341 catcattatt gatggtgttg aaagattaca tgggacacgt catcaggtga ttccagaccg
    20401 tattgaagct ggaacatata tatctttagc tgctgcagtt ggtaaaggaa ttcgtataaa
    20461 taatgttctt tacgaacacc tggaagggtt tattgctaag ttggaagaaa tgggagtgag
    20521 aatgactgta tctgaggaca gcatttttgt cgaggaacag tctaatttga aagcaatcaa
    20581 tattaagaca gctccttacc caggctttgc aactgatttg caacaaccgc ttacccctct
    20641 tttactaaga gcgaatggtc gtggtacaat tgtcgatacg atttacgaaa aacgtgtaaa
    20701 tcatgttttt gaactagcaa agatggatgc ggatatttcg acaacaaatg gtcatatttt
    20761 gtacacgggt ggacgtgatt tacgtggggc cagtgttaaa gcgaccgact taagagctgg
    20821 ggctgcacta gtcattgctg ggcttatggc tgaaggtaaa actgaaatta ccaatatcga
    20881 gtttatctta cgtggttatt ctgatattat cgaaaaatta cgtaatttag gagcggatat
    20941 tagacttgtt gaggattaaa ccgtagaggt gtttatgaat atttggacca aattagcaat
    21001 gttttctttt tttgaaacgg atcgcttgta tttgcgtcct ttctttttta gtgatagtca
    21061 ggacttccgc gagatagctt caaatccaga aaatcttcaa tttattttcc caacgcaggc
    21121 aagtctggaa gaaagtcaat atgcactggc caattacttt atgaagtccc ctttgggagt
    21181 gtgggcaatt tgtgaccaga aaaatcaaca aatgattggt tctattaaat ttgagaagtt
    21241 agatgaaatc aaaaaagaag ctgagcttgg ctattttttg agaaaagatg cttggtcgca
    21301 aggatttatg acagaggttg ttagaaaaat ttgtcagctt tcttttgagg aatttggctt
    21361 aaaacaatta tttatcatta cccaccttga aaatgaagct agccaaagag ttgctcttaa
    21421 gtctggattt agtttgttcc gtcagtttaa gggaagtgat cgttacacaa gaaaaatgcg
    21481 ggattatctt gaatttcggt atgtaaaagg agagttcaat gagtaagcat caggaaattc
    21541 taagctattt ggaggaatta ccagtaggta aaagggtcag tgttcgtagc atttcgaatc
    21601 atctaggagt tagtgatgga acagcctatc gagctattaa agaagctgaa aaccgtggaa
    21661 ttgtggagac ccgtcctaga agtggaacaa ttcgtgttaa atcccagaaa gttgctatag
    21721 atagattaac gtttgctgaa attgcagaag tgacttcttc tgaggttctg gctgggcaag
    21781 aaggtttaga gagagaattt agtaagtttt caattggtgc catgactgaa caaaatatct
    21841 tgtcttacct tcatgatggg gggctcttga ttgtcggaga ccgaacccgt attcagttgc
    21901 tagccttgga aaatgaaaat gcagttctgg ttacaggggg atttcaggtt catgatgatg
    21961 tgcttaaact ggccaatcaa aaagggattc ctgttctaag aagtaagcat gataccttta
    22021 ccgtcgctac catgatcaat aaagccttgt caaatgtcca aatcaagact gatattctga
    22081 cagttgagaa actttatcgc cctagtcatg agtatggttt tctgagagag acagatacag
    22141 ttaaagatta tttggacttg gttcgtaaga atcgtagcag ccgtttccct gttatcaatc
    22201 aacatcaggt cgttgttggc gttgtaacca tgagagacgc tggtgataaa tcaccaagca
    22261 cgacaattga taaggttatg tctcgtagtc tatttttggt tggattatcg acaaatattg
    22321 ccaatgtgag tcaacggatg atcgcagaag actttgaaat ggtaccagtt gttcgaagca
    22381 atcaaacttt gcttggcgtt gtgacgcgac gagatgtcat ggagaagatg agccgttccc
    22441 aagtttcggc tctaccaact ttttctgagc agattggaca aaagctctct tatcaccatg
    22501 atgaagtagt cattacagtg gaacccttta tgctagaaaa aaatggagtt ttggctaatg
    22561 gtgtattggc agaaattctg acccacatga cccaagattt agttgttaat agtggtcgca
    22621 atctcattat cgagcagatg ctgatctact ttttgcaggc cgttcagata gatgatatat
    22681 tgcgcattca ggcacggatt attcatcata cgagacggtc agctataatt gattacgata
    22741 tttatcatgg tcaccagatt gtttcaaaag caaatgtgac tgttaaaatt aattagaaac
    22801 taggagaaaa gatgataaca ttaaaatcag ctcgtgaaat cgaagctatg gacaaggctg
    22861 gtgattttct agcaagtatt catataggct tacgtgattt gattaagcca ggcgtagata
    22921 tgtgggaagt tgaagaatat gtccgccgtc gttgtaaaga agaaaatttc cttccacttc
    22981 agattggggt tgacggtgcc atgatggact atccttatgc tacctgttgc tctcttaacg
    23041 atgaagtggc tcacgctttc cctcgtcatt atatcttgaa agatggtgat ttgctcaaag
    23101 ttgatatggt tttgggaggt cccattgcta aatctgacct aaatgtctca aaattaaact
    23161 tcaacaatgt tgaacaaatg aaaaaataca ctcagagtta ttctggtggt ttagcagact
    23221 catgttgggc ttatgctgtt ggtacaccgt ccgaagaagt caaaaacttg atggatgtaa
    23281 ccaaagaagc tatgtacaag ggtattgagc aagctgttgt tggaaatcgt atcggtgata
    23341 tcggtgcggc tattcaagaa tacgctgaaa gtcgtggtta cggtgtagtg cgtgatttgg
    23401 ttggtcatgg tgttggccca actatgcacg aagaaccaat ggttcctaac tatggtattg
    23461 caggtcgtgg actccgtctt cgtgaaggaa tggtcttaac cattgaacca atgatcaata
    23521 caggcgattg ggaaattgat acagatatga aaactggttg ggcgcataag accattgacg
    23581 gtggattgtc atgtcagtat gaacaccaat ttgtcattac gaaagatgga cctgttatct
    23641 tgactagcca aggtgaagaa ggaacttatt aataaaaagt gaaaagacta ctggaagttt
    23701 attatgaaaa aatccagtag atcttttcat aataaaacgc attgtatcaa gtgttagggg
    23761 ctgatatcat gcgtttttct gcttttaaga ttttttccaa ctctgtttgt aagcgcatca
    23821 taacaaaggg tctaggattc agggctctcc tcctatatac tattagtaaa gtaaaactaa
    23881 gggaggatat tttagtgtcg cagtctattg ttcctgtaga gattccacaa tattgtcgtt
    23941 ttgattctaa aaagagaaat ggaattctgt ttaatgttcg tattgccaat cttaaattta
    24001 ccttttttag attagacttc ctgcgaaaca aaacatggta tagtagttct atgaatgatg
    24061 aagcaagtaa acaactaact gatgcacgat ttaagcgtct tgttggtgtt caacgcac
//
LOCUS       DAVBGY010000025        24010 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVBGY010000025 DAVBGY010000000
VERSION     DAVBGY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24010
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.025"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(181..1359)
                     /locus_tag="VQ620_001068"
     CDS             complement(181..1359)
                     /locus_tag="VQ620_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET2763703.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALFYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            1639..2901
                     /locus_tag="VQ620_001069"
     CDS             1639..2901
                     /locus_tag="VQ620_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2763704.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            2904..3800
                     /locus_tag="VQ620_001070"
     CDS             2904..3800
                     /locus_tag="VQ620_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HET2763705.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            3805..5490
                     /locus_tag="VQ620_001071"
     CDS             3805..5490
                     /locus_tag="VQ620_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HET2763706.1"
                     /translation="MKNKKLISSLFILFLLIFIHVKVFANENLIKNSNFEQGELFWNS
                     RNRGIINSNESYSNGHYSGMIPSTAINDNRADGYIGQVIDIEPNEDYLVSAFAKVDIS
                     GAEGYFTARWFDNNKQGEIVQNNIGEAVDQTVNTTEWKKYMFSFNSGKHDKVLIQLVK
                     WSEDDTTKKSNIFIDNVEMYQLSKGNSYKKIWRDDFDGEQLNKKYWGYELGSIRGWEQ
                     QHYVRSDENVFLRSGNLVLRATNRSKEDQYFNPRNNHRKVVYNSGSVRTHGKVEFLYG
                     KLEMRAKLPKGQGVFPAFWTLGSDFTLDGKINPVQGRGWPSTGEIDIMELVGERNSNG
                     SGNKTVYQTLHYGQSDNDDGKFAGRGTAFKLSSGNFNDSYHTFSIDWYKDYIVWMVDN
                     HIVRKVYYGSDNIAKKIFNRPQYVQLNLAMGGSWPGVVDNNLSGTEFVVDYVSYSRND
                     LQQHQAEEYYSQSPKINGAKDIVINRGDVPDLLKGITTNSGYELDYSIENEPMFNNFG
                     GNTSVDLLIRNKTEKEKISQMPPGVYNLHYTSRPKHLDYESKVDRKTVTLTIK"
     gene            5503..5820
                     /locus_tag="VQ620_001072"
     CDS             5503..5820
                     /locus_tag="VQ620_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2763707.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            5837..6154
                     /locus_tag="VQ620_001073"
     CDS             5837..6154
                     /locus_tag="VQ620_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2763708.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            6164..7519
                     /locus_tag="VQ620_001074"
     CDS             6164..7519
                     /locus_tag="VQ620_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2763709.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            7842..9317
                     /locus_tag="VQ620_001075"
     CDS             7842..9317
                     /locus_tag="VQ620_001075"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET2763710.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYVSDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTSLKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            9314..10162
                     /locus_tag="VQ620_001076"
     CDS             9314..10162
                     /locus_tag="VQ620_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET2763711.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDRFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            10177..10947
                     /locus_tag="VQ620_001077"
     CDS             10177..10947
                     /locus_tag="VQ620_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET2763712.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(11225..11512)
                     /locus_tag="VQ620_001078"
     CDS             complement(11225..11512)
                     /locus_tag="VQ620_001078"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2763713.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCSRQRRLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            11710..11988
                     /locus_tag="VQ620_001079"
                     /pseudo
     CDS             11710..11988
                     /locus_tag="VQ620_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12098..12301)
                     /locus_tag="VQ620_001080"
     CDS             complement(12098..12301)
                     /locus_tag="VQ620_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET2763714.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(12332..12940)
                     /locus_tag="VQ620_001081"
     CDS             complement(12332..12940)
                     /locus_tag="VQ620_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2763715.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            complement(12979..13149)
                     /locus_tag="VQ620_001082"
     CDS             complement(12979..13149)
                     /locus_tag="VQ620_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HET2763716.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            complement(13161..13727)
                     /gene="amaP"
                     /locus_tag="VQ620_001083"
     CDS             complement(13161..13727)
                     /gene="amaP"
                     /locus_tag="VQ620_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HET2763717.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            complement(13811..13975)
                     /locus_tag="VQ620_001084"
     CDS             complement(13811..13975)
                     /locus_tag="VQ620_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET2763718.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            14418..15899
                     /locus_tag="VQ620_001085"
     CDS             14418..15899
                     /locus_tag="VQ620_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2763719.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSS
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            16261..17256
                     /locus_tag="VQ620_001086"
     CDS             16261..17256
                     /locus_tag="VQ620_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2763720.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICN
                     ADSDSQNEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSENVCYVTSA
                     NYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKE
                     PLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKT
                     SVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIVIPTYFGGDKTTKKI"
     gene            17383..18693
                     /locus_tag="VQ620_001087"
     CDS             17383..18693
                     /locus_tag="VQ620_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET2763721.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGSGGGYEDKVNAAITTNSLPDVITL
                     DGPNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYN
                     KKMFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPAIDFRINSNDEMLPYAYM
                     PLIWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYP
                     MLLSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASE
                     FVKFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVA
                     YPQVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            18789..19691
                     /locus_tag="VQ620_001088"
     CDS             18789..19691
                     /locus_tag="VQ620_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2763722.1"
                     /translation="MSKETVVYTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYAFTDYYLLTPDERKFVGLENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMVYYIYQQGFTDRLVGYSSS
                     IALVFTTLIGMISLVQRRVLKEDD"
     gene            19693..20538
                     /locus_tag="VQ620_001089"
     CDS             19693..20538
                     /locus_tag="VQ620_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2763723.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFSGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRLAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
     gene            20548..21867
                     /locus_tag="VQ620_001090"
     CDS             20548..21867
                     /locus_tag="VQ620_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET2763724.1"
                     /translation="MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(22075..22147)
                     /locus_tag="VQ620_001091"
     tRNA            complement(22075..22147)
                     /locus_tag="VQ620_001091"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22112..22114),aa:Lys,seq:ctt)
     gene            complement(22411..23682)
                     /locus_tag="VQ620_001092"
     CDS             complement(22411..23682)
                     /locus_tag="VQ620_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET2763725.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWIKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
BASE COUNT         7587 a         3635 c         4758 g         8030 t
ORIGIN      
        1 gatcgattcg tcctgttatt atttcatttt actatacatt atgaaaaagg ggagatagga
       61 gtataattcc aaatataaag aaagcgaaca aaacagaatt ctgattttca gaaaacaggt
      121 ttattcgctt tttatgatat actccctctt tttagcctat ttattaaatg taatagaaaa
      181 ttaagaatgt agaattgtat gatacaaaca aagtgcaata gctgagtgtg cggctgtaaa
      241 atcattgtaa ggttcaataa tgacttcagg taggcgatta tagagcagtt tgggtttgta
      301 gttcatttca ctatatagtt tttggatgat gtgttgattt gttagtaaag gactatgtag
      361 gtagattttt tgagagtcaa tcatcatact gatatttaaa atggtttgac taagatagaa
      421 taaggcttga tggataagtg tgattattcc agtgtcacct aattgataag cggttaaaat
      481 aacttggata tcaatatcat cggcattttt gactaaacta ggaagtagag aatagggaga
      541 ttgatgatat agaatttttg attttttgat taaccaagat tctccagcaa aagtttgtaa
      601 gcatcccttg cgcccgcagc tacattcttc tccctcagat gaaacaactg tatgccctat
      661 ttctccaatc attagatttc ctttcccata aatgtttccg tcgtagatat aagaacagtg
      721 catacctcta gcaaaatgaa aataagcaaa attagggtca ttctgttggc gactaaatag
      781 gcgtttacct atagccatac agttaacatt gttagagaaa aacaaaggct tatcaaagtg
      841 ggattgaatc atttctaagt cgatatgttg ccataaagga ttgtttgttg ttattttata
      901 atcatctaaa tagcgaccag gtagcgcgat tccaattgct tctatttcat aatcagagca
      961 gttatttaag aattgcttta gtgtctggtt aatgagttga tttccctttt cttggattaa
     1021 ctgtttagta acaatttctt tctcttcctt taagatattt cctagattat ctcctaaagc
     1081 aaaagtgaaa tgtttttcag atagttcaca accaatgtag taaaatcgtt tagcttgaat
     1141 gtctagcagg attttttttc tccctacgct tgtatcgtgt tcgtcttcac caagttctaa
     1201 gagaatattt tctttaatca gatcatttgt gatactgcta gttgttgcag gtgttattcc
     1261 tgttttagta gcgatttcaa ttcgtgaaat gggacctaat gtgtaaactg tttccagtat
     1321 tttagctctt agttgtaatt gtttcttaga taaggtcata ttagttttct tcctagttgt
     1381 cagattctta aaatcctatg attttttgct atggataaat gtagatgtta atctttttaa
     1441 gcttttcatt acttactggt ctctttttat tatatattga taataaaaaa taatcaaatg
     1501 tttacacaaa tccttgacaa ggctttctta tagttgtaaa atataaataa caaaagataa
     1561 tcaaataaca aattgacgtc aaaaaataaa gataattgat aacttttgat aataaaatat
     1621 atttaagagg aaaaggttat ggaaaaatta ttacaagaaa agttacttcc agtagcggct
     1681 agattgggta ataataaagc gttggtttct attcgagacg gtattactct aactatacct
     1741 ttattgttga taggttcatt actaatggtt attgcaagtt ttcctattcc aggatgggaa
     1801 aaatatctgg gtgatattgg tgttgctgac tatttatgga agggtgttga tagtagtttt
     1861 ggattacttg gtcttgtagc tagttttggt atcgcttact ttatggctag acaatacaaa
     1921 gttgatggta ttcctgctgg tattgtgtca ttatcatctt ttattacagt tacgcctttc
     1981 attagaggag aggctggagc aggaatgcct accgctttca tggcatcaaa aggtttattt
     2041 gttgctatga ttttaggatt gattaatggc tatatctacc aatggtttat aaatcataat
     2101 atacagataa aaatgccgga tggtgttcca ccagcagtat ctaaaagctt tagtgccatt
     2161 attcctggtg cagtgactat tgttggttgg ttgattgttt atgcaacttt agataaatta
     2221 agtttaccta atcttcatga aattgctcaa gtagctttgg gaggtccact tggactttta
     2281 ggaaataatg ttattggtct tcttatctta attttcctta atagtagttt ttggtttgta
     2341 ggattacacg gaggaaatgt tgttaatgca gttatgaaac cgttatggtt agctaatcta
     2401 gatgcaaata aagtagctta tcaaactgga gaaactcttc caaatatttt tactagcgta
     2461 tttatggata attttgtatt cataggtggc ggtggtgcaa ccataggttt agttcttgcg
     2521 ttaggatatt tagcacataa gaagaaagct agtaaacaac taaaaacttt ggcacctata
     2581 actgttatac ctggattatt taatataaat gaaccagcaa tgtttggcgt tcctattgtt
     2641 ttgaacattt tgttactagt tccatttatc ttagctccaa tgtttaactt actagtagcg
     2701 tggggagcaa tggcatcagg tttggttccg ctaacttata cagatccagg ttggactatg
     2761 ccacctgtta taagtggttt acttgctaca ggaagtattt ctggttcatt attacaaatt
     2821 gtattgattg ttttggatgt cttactttat ttaccatttg taatagctat tgaaaaacgg
     2881 tttaagttat tggaggattg agaatgaaac gtttaattag tgcaaatcca tctgagatat
     2941 tacagatgaa tgctgaagaa ttaaaacaaa gtattttagc aagtgaaggt agagttgttc
     3001 tatctgaaaa tgtagttact cgtgagacat ttgttgggga tataactaat tctgaaattg
     3061 ctagagcttt tggagctgat atgattttat tgaattgtgt tgatgttttt gagcctaaaa
     3121 tttatgcttt ggatagttca ggtgatgatg ttattcatcg cttacaccag cttgttgctt
     3181 gtccaattgg tgtaaatttg gaaccgattg acccatctgc aaagatgcta gaggaaacac
     3241 aggaaattgt tgcaggtcgt gttgctagtg ttgaaacatt gaatcgaata gaggagttag
     3301 gttttgactt tgtctgtttg actggaaatc ctggaacagg agttagcaat cgagaaatca
     3361 ttaaggctgt tcaaactgct aaggaaaact tttctggttt gattattgca ggtaagatgc
     3421 acggggcagg agtgaatgag cctgtggcag agctttctgt cgcagagcaa ttgttggaag
     3481 caggtgcgga tgtgatactt gttccagcag ttggaaccgt tccagccttt catgaccaag
     3541 agttgcgtga agtcgttgat ctcgttcata gtaagggtgg gctagtactg agtgctattg
     3601 gtactagcca agaaacatct gatacagata ctatcaagga aattgcactt agaaataaaa
     3661 tttgtggagt tgatattcaa catataggtg acgcaggata tgggggactg gcaacagtcg
     3721 ataatattta tgcattgagc aaggcaatta gaggagtgag acatacagta tctcgcttgg
     3781 ctaggtcagt aaataggtga taaaatgaag aataagaaat taatttcaag tttatttata
     3841 ttatttttat taatatttat tcatgtgaaa gtttttgcta atgaaaattt aataaaaaat
     3901 tcaaattttg agcaaggaga gctgttttgg aactctagaa ataggggaat aattaattca
     3961 aatgagagtt atagtaacgg acattactca ggtatgattc cttctactgc aattaatgat
     4021 aatagagcag atggatatat tggacaagtt attgatatag aacctaatga agattattta
     4081 gtatctgctt ttgccaaagt agatattagt ggagcagagg gatattttac cgcaagatgg
     4141 tttgataata ataagcaagg agagattgtt caaaataaca ttggagaagc tgttgatcaa
     4201 acagttaata caaccgaatg gaaaaaatac atgttttctt tcaattccgg taaacacgat
     4261 aaagttttga ttcaattagt gaaatggtca gaagacgata ctacaaaaaa atctaatatt
     4321 tttattgata atgtagagat gtatcaactg tctaaaggaa attcatataa gaaaatttgg
     4381 agagatgatt ttgatggcga acaattaaat aaaaaatatt ggggatatga attaggatca
     4441 attcgtggtt gggaacagca acattatgta agaagtgatg agaatgtttt tctacgttcg
     4501 ggaaatttag ttcttagagc tacgaatcgc tctaaggaag atcagtattt taacccaaga
     4561 aacaatcaca gaaaggttgt atataattca ggaagtgtta gaacacatgg gaaagtagaa
     4621 tttctttatg gtaaattaga gatgcgtgca aaattaccga aaggtcaagg agtttttcct
     4681 gctttctgga ctcttggatc ggattttaca cttgatggga agattaatcc tgttcaggga
     4741 cgaggatggc cgtcaacagg tgagatagat ataatggaat tagttggtga acgaaattca
     4801 aatggaagtg ggaataagac agtttatcaa acgcttcatt atggacagtc ggataatgat
     4861 gatggaaaat ttgcaggacg tggtacagcc tttaaattat cgagtggaaa ctttaatgat
     4921 tcgtatcata ctttttcaat tgattggtat aaagattata ttgtatggat ggttgacaat
     4981 catattgtta gaaaagtata ttatggatca gataatatag cgaaaaagat ttttaataga
     5041 cctcagtatg ttcagttaaa tttagcgatg ggaggaagtt ggcctggggt agtggacaat
     5101 aatttatcag ggactgaatt tgtagttgat tatgtttctt actctagaaa tgatttacaa
     5161 caacatcaag ctgaagagta ttatagtcaa tctcccaaaa taaatggcgc taaggatata
     5221 gtaataaaca gaggagatgt tcctgattta ttaaaaggaa ttacaactaa tagtggatat
     5281 gagttagatt attctattga aaatgaacct atgtttaata actttggagg gaatacttcg
     5341 gtagatttat taattcgcaa taaaactgaa aaagaaaaga tttcacaaat gcctccagga
     5401 gtttataatt tgcattatac tagtcgtccc aaacatttag attatgagtc taaagttgat
     5461 cgaaaaacag taacattgac aataaaataa aaggagtaag ctatggctaa agtaacaatt
     5521 atgttagcgt gtgcagcagg tatgagtaca agtctgctag tgacaaagat gcaaaaggca
     5581 gcagaagata aggggttgga tgcagaaatt tttgcagttc cagctcctga agcagaagaa
     5641 attgtagcaa caaaagaagt aaatgtgttg cttttaggcc cccaagttcg ctatttacta
     5701 ggggactttc aagaaaaact aaaagataga cagattcctg tggcggttat tccgatgaca
     5761 gattacggaa tgatgaatgg ttctaaagtt ttagatttag ctgaaagttt attagactaa
     5821 gattgaggag aatgttatgg atgaaagtaa tttagaatct gtaatggggc taattatgta
     5881 tggtggggaa gccaaaagta atgctatgga ggctattcag gcagcaaaaa aaggggattt
     5941 ctctaaagcc aatcgaagat tagctgatgc gaatgctgcc ttattacagg cgcataaggc
     6001 tcaaacagaa atgttgacaa gagaggcaca gggggaagaa acatcaatta gcctcttgat
     6061 ggtacacgcg caagatcatt taatgacgag tctgactttt gttgacttgg cgaaagaagt
     6121 ggtagaagta tacgaacgat ttgaaaaaaa ttaggagttt agtatgaata caatgttgga
     6181 taaaatgcaa gaaaaacttt ctccaattgc aatgaaagtt gggaatcaga agtttttagt
     6241 tgctttacgt gattcatttg tggggactat gcctgttatt atgacaggtt ccattgcttt
     6301 gttattaaat gcttttctag tggatttacc acaacaattt cacctagaaa gtattacgaa
     6361 aacctttcag tggcttgttg acatcaataa tttggtattc aagggaagta taccaattgt
     6421 ttctttgttg ttcatctatt gcttgggagt gaatatcgct aagatttaca aggttgatac
     6481 agtttctgct ggtttggtat cacttgcttc gtttgtcatt tcgattggaa gtacagttac
     6541 aaagagtttc cctttggcaa atgtaggaga tgtgaaatta gatcagatct tacaaggaat
     6601 tgataatcta gcatttgatg gtaaaaatct gatggtaact attgggaatg tgattccagg
     6661 aaaccatatt aatgccagag gctactttac agccatgatg attggatttt tagcctctat
     6721 tattttctgt aaagtaatga aaaagaactg ggtaatcaag ttaccagatt cagttccccc
     6781 tgcgattgct aagccgttca cttctatcat tccaggattt atggcgatgt atatagtagc
     6841 catcttgact tatgttttcc atttacttag caatgactta ttgattgatt gggtttacaa
     6901 ggtgttacaa acgccattac taggtttgtc tcaaagtttc tttgcagtta ttctgatgat
     6961 ctttttaaat aagttattct ggttttttgg tctccatgga ggaaatgtat tggctcccat
     7021 catggaaggg ctgtttggag ttgctatgtt agcgaatctg gatgctttcc aaaaaggtga
     7081 accgattcct tatatatgga caagtgggtc ttttggggcg tttgtctggt ttggaggatt
     7141 aggattggta cttgctattt taattttttc aagaaacagt cattatcgaa aagttgctaa
     7201 acttggtcta gcaccagttc tgtttaatat tggtgagccg gtcaattatg gtttaccagt
     7261 tgtcttgaat cctttgctat ttataccatt tgtacttagc ccggttttca tggcaacggt
     7321 agcttactgg gcaacaagct ggggtcttgt ttcacctgtt acacaaaatg ttacttgggt
     7381 aatgcctcca attttatatg gtttcttctc tacagcattt gattggcgtg ccatcatctt
     7441 atcagttgtt tgtttgatta ttagtgtctt gacttatttc ccatttgtga aaatggcaga
     7501 taaaactgaa ttgagttaat ggtcttactt gcgttttttg tgataaaata gaaatagatt
     7561 acgtgtaaga tgagttaggt gtaggatttc ttatctccac taaaattgtt attagaccaa
     7621 ggtttatagt ttgttgaagt tggaatagta cactgtaact tctaaaacag tactagaaaa
     7681 tgatttgaat ttcctaatcg atttagtcat attttagttc attgttctat aaaacataac
     7741 aagaatgtta ttgctttata tagagagatt gatatagaaa ggggtgcagt aaaggtagat
     7801 atcttcttgt gcctcttttg ccatattaga aaggagattt tatggttcag acaaaacagc
     7861 caaatattat tttgattgtt gtagatcaaa tgagagcaga cgccttatcc ttgaatagta
     7921 aggataagct agttagcact ccaacattag atatgatggc tagtgtaggt tataattttg
     7981 aaaatgccta ttctccagtt ccttcttgcg ttcctgctag ggcagcttta ttgactggct
     8041 tagaccaaga taaaagtgga cgtgttggtt atcaagatga ggtgccttgg aattttacta
     8101 atactcttcc aaaagtcttt aaagatatgg gttatcaaac agagtgtatt ggtaagatgc
     8161 atgtttttcc ttccagacag agacttgggt ttgatcatgt cttacttcac gatgggtatc
     8221 tacatgttga tagaaagtat gataaggctt atggaagtca atttgattat gttagtgact
     8281 atctagcctt tttaaagggt aaagttggtt atgatgttga tttgattgat gacggaatgg
     8341 attgtaattc ttgggaagct agaccttggg ataaagatga gaagttacat ccaacaaact
     8401 gggttgtgag tgagtccatt tcatttttac aaagaagaga tccaacggtt cctttttttc
     8461 tcaaaatgtc ttttgaaaaa ccacatgctc cattaaatcc cccaaaatac tattttgata
     8521 tgtatatgga acgcttgcct caatttctcg atttgcatat cgggaattgg gaagtattag
     8581 aaaagcaaat tccgtctatt tatgctttaa gagggaaatt aaaggaagat gatcaaagaa
     8641 gaatggttgc tgcttatttt ggattgatta ctcatataga ccatcaaatt agtcgctttt
     8701 tgacttccct aaaagaattt cgtcatgata aagatacgat tatctggttt gtttcggatc
     8761 atggagatca gcttggtgaa cattatctgt ttagaaaggg atatccttat cagggaagta
     8821 tccacattcc ttcgtttatt tacgatccgg ctggattgat agctggtaat aggggaacaa
     8881 tcaagcaatt agtgaaaatt caagatattt ttccttcact cgtggatttg gcaggaggaa
     8941 ctactacaga tgaactagat ggacggagtg ttaagaattt attatttgga cagtacgagg
     9001 ggtggcgaac agaatttcat ggtgaacatg ctttaggaaa agattctagt caatatatct
     9061 tgacagacca gtggaaattt atctggtttc ctgttttaaa tcattatcag ttatttgata
     9121 tgaaaaaaga tccgcatgaa atgaatgatt tgtatccaag cgaaaaatac caacctattg
     9181 ttcgtcagat gaagaaaaaa ttagtagact ttcttagata tagagaagaa ggttttgttg
     9241 tggatgaaga gttagttcct gttgaacttt ctaaaataac gccaactcta acgaaaacgg
     9301 gggatagtca atcatgaaaa tgaaaaaaat caagggtggg acatttatga tgggaacaaa
     9361 ttctgaagag ggctttttag atgattttga aggtcctcaa gtcgctgtta gtgtaaagga
     9421 tttttccatt gcggatactc cagttacgaa tcaagaattt gctcaatttg tgaaagaaac
     9481 aggctataaa acactagctg agcgacaaga gtggtctttt gtttttatat tgttcgttcc
     9541 agaggcagaa agggagggat atcctcatcc tgctggggct ccgtggtggt tacaagttcc
     9601 gaacgcttgc tggaaacatc cctacggtga gaacagtaat ctagtgggtt tagaagacca
     9661 tccagtagtt catgttgctt tagaagatgc attggctttc tgtaattgga gcggaatgtc
     9721 tttacctact gaagcacagt gggaatatgc tgctagagga ggaagacaat ctgaatatcc
     9781 ttggggagat actcttttag aaggtggata ttatcatgcc aatacttggc aaggaagatt
     9841 tccttatgaa aatactgcct tagatagatt tataggaacg gctcctgtct atgaattttt
     9901 acctaatgat tttggtctat atcaaatgat tgggaatgtt tgggaatggt gtcgcaatcc
     9961 gagatatacc ttattagcta gttttaatga agatgactat gagttgccaa aatatgggat
    10021 acaagatgag gagtatgcta tcagaggagg ttccttttta tgtcattgtt cttattgtaa
    10081 tcgataccga gtagctgcta gaaatggatg tatctctact tccactagta gccatctagg
    10141 ttttagatgt ttgaaagaat aggatggact taaaaagtgg ttgagattat ttattttcta
    10201 atcattataa tagctagtgg attgggttct atatcaggca tgggtggtgg aattataatt
    10261 aagccactta tggatagttt tggataccat tcggtcagtg atattgcttt ttattctagt
    10321 ttctctgttt ttataatggc gattatttca acaatcaaaa gattttcaca aagtaaagag
    10381 ataaaatgga gattgatttt tacggtatcc ttttcttctg ttttaggagg atttctaggt
    10441 catcttattt ttcaagtttt gttatctcag ttatcagtac ggctagtatc cattgttcaa
    10501 atgattttac tatttgtgat gctacttgta tcatttgttt taacagattt taaaaaaact
    10561 tatcaatttg ataagatagg attctatatg atttgtggtc ttctattggg cttaatttct
    10621 agttttttag ggattggtgg tggtccgctc aatgtatctt tattgatggt gtttttttct
    10681 atttcgataa aagaagcaac gatgtattct cttgcaatta ttttcttttc ccagctgtct
    10741 catttggcta caattgttgt ggttacaggt ttaaatcagt atcatttagc acctgttcca
    10801 gttatttttc ttgcttctat atgcggaggg gtattgggga cggttgtatc aaaagtctta
    10861 cctgaaaatt gggtaagata ctgttttaaa gggatgttat tctttgtcat gggaatgact
    10921 ttatataatt tatttcatat tttgtaaggt cagtgtgaaa atatttaaat agacttcctg
    10981 taaaaactgg atttaacgcc ctccatttgt caaattaagc tagacattct taccaactca
    11041 gaaaatacct gatgaggtat ttggtatcca aaggatttag gtgggatgtg atttcttgag
    11101 gctgggaaaa agtctttaaa attaaaaaat gcataatatc aggtgttgga aaaactttga
    11161 tactatgcgt tttattgtgg gaatatttat ttcattttct cctgaaattg agtttttgtc
    11221 cagcctaaaa ttcttgactg acttcgttga aatctgtctg ctttggcaag tcatgacacc
    11281 ttaaaagtct atttgaatgt tcgttgagac gccgctgtct tgaacatcct agattcgcaa
    11341 aaaagctctc actatcgtga tgattggaaa tatctttcca ttttgaaaac tcttttccac
    11401 agtcaaacgt aatagattta aagaggttca ctagaaattg tgccagccac ccttggatgg
    11461 attctgccat atcctttgcc ttacgactct tggtcttcaa actgacaatc aattgactat
    11521 acttgttcgc tcacaaatag gagagaactg tttacgaaaa tctaatgaat atgccacaag
    11581 aacattatac cgcaacgtgt actatttttg gttcatttta ctatagaaac tattgaagaa
    11641 cctgtacgaa aatatatcat gaaaaagggg agatagaaag caaacagcct tgaaatcaat
    11701 gttgactgcg attatgaacc tgaatcagcc cctcaaaagg gactgtatat gaatgaacgt
    11761 taccagtgtt taaaaactaa agaatatcag gcacttttat cttccaaggg tagacaaatt
    11821 ttcgctaaac gtaagattga tatgaaatct gtctttgggc agataaaggt ttgtttgggt
    11881 tataagagat gtcatctgag aggtaagcgt caagtgagaa ttgacatggg attcatactc
    11941 atggccaaca acctgctgaa atataataag agaaagaggc aaaattaaaa agctagagtt
    12001 ccgcaattgg gaatctctag ctttttgttt tctgagaatc atcttgactc agactctttt
    12061 gactaatgtt accaaaaaga taaagtaact tttaacctta tttatcgtcg ccactcaaca
    12121 tgtttttaac accttctaca gcaccttcta cagcgtcttt agcgtcttcg gcaacttctt
    12181 ttactttaga aacaactttt tcagctgctc cttctttttc cattttttca tcaccgatgg
    12241 ctttcccaac accttcttta atagaacctt tagcttgatt taatttttct tctactgaca
    12301 tgataatttc tcctgttata tttattttat cttagtttac acgagtgttt tcgtgagata
    12361 ctacaccatt tgctgcacct ttaacagctt ctacaccttc gctaactttt tcttgaacag
    12421 ttgagaaacc agatccaaga ccagatttag ctttttcgaa ttgttctgaa gtgaattctc
    12481 ctgttgattc agcaacgtca gatacgcgat cttgaaggct tactgagtct gcttcatgct
    12541 gttctttagt tttgatgtcg acaacgttta cgttgatttc aacaatttcc aagtcagtca
    12601 ttttagcaac ttctgaagat acgatttctc tgatttctga atataaagct ggaacatttt
    12661 tttggtactc aacaataacg tttaagtcaa ctgcaacttg tgttttacca acttctacgt
    12721 taacaccact tgttacgtca tcgctgttaa cgattttttc tttaagattt gagaagaaac
    12781 caccatcgat tcccaaaaga cctgaaacgt tttctagtga aagaccaatg attttttgga
    12841 taactttatc ttcgtaagta agttcccctt tgatttcgtg agctacaaca gtagcatctt
    12901 tcttttctac gttagtattt gtgttttttt cgtttgacat atgaaactcc tttaattaaa
    12961 ttagtaattt ttattttttt atttatctat atagtttttt tcgatatata atccagctgc
    13021 aactcccagt gctcctaaaa tcaatacaaa tattgttttg aagaagccaa aggagacaat
    13081 caaacaagcc agaaatacgc ctatgagacc agcgataatt ggatatcgat attgtttaag
    13141 ccattccatt ttttacttcc ttacttcaca cgactaacag tctttttgtt ttgaggtttt
    13201 ggttggtaat tttttactgc aacttcaagt tttacttgac gctcaatacc aaaaaactgc
    13261 ttcaatccat tagttatttc attttgaatt atttggcatc tgtcagcgat gttgtctgaa
    13321 ggaagaattt taccttctac atgaacgaaa catttatttt ttcgtaaatt tacatgaaca
    13381 gttgggttct tgatcaatct atgatcactc accaaacttc taacaaaacc ttcgattgcc
    13441 gaattcttta attttaatgt atcgttttta gtttcaagtt ggatttccaa gtaacgttta
    13501 ggataaaaca tcacaactaa aatggataat aaaactaaag ttgatagaac cactgtcccc
    13561 caaaagacat atctagcaag atagaattca actacggagt tctgtctcca gctaagtaga
    13621 tgaataccta gatcactaac ttgatgataa tctatcaaaa caggaaggaa aattgtcaag
    13681 attaaaatac agaaaataat gaaacatatt ttctttgctt ttgacatata caatcctttc
    13741 gatttaacat ttaaactatt tttcccgtca ttgatttaaa aatagtttgc taactttact
    13801 ggcgaaaagt ttactttcta cctgtaaaga atgacactac agtaatcaca atcgctgcac
    13861 ctacaataga tgggagcaaa gccattccag cgattgatgg accccaacta cctaaaagag
    13921 attgtcctgc atatgccccg actaaaccag cgaatacatt tgcgatgatt cccatagaac
    13981 tactttttta gtgattttac ctgccaagag accaataaga cctccagcaa taattgatca
    14041 caacactgcc taccctcctt ttttatttta atatcaaaaa atcaatttct ttgattaata
    14101 ctattatata actttaatat agaaaagtca attatttcga ttgttattat tatataattt
    14161 taaaaaatca aattctttaa ttgttgctat tatataattt ttaaaattaa aaatataatc
    14221 tttagacttg aatttgattc tgtattcacg ccctcaatag acggtaaaat aagaaaattg
    14281 tttatatatt gtctccgtag tgttattata cgaaataaaa gattttagga aaagtcgtta
    14341 tatcatgcta tacctattct ttgtggtata attgcaagag gtttaatccg ataatttata
    14401 aagagagaaa gatattcatg agagatttat tatctaaaaa aagtcatagg caattagaat
    14461 tattagaatt attatttgaa cataaacgtt ggtttcatcg ttctgaacta gcagagttat
    14521 taaattgtac agaacgtgca gtcaaagatg atctatccca tgttaaatct gcttttcctg
    14581 acttgatttt tcattcttct actaatggta tacgcattat taataccgat gatagtgata
    14641 ttgaaatggt ttaccatcat ttctttaaac attcaactca tttttcgatt ttagaattca
    14701 tcttctttaa tgaaggttgt caagctgaga gtatttgtaa agaattttat atcagttcat
    14761 cttcgctcta tcgtattatt agccaaatca ataaagtgat taaaaggcaa tttcaatttg
    14821 aagtcagtct gacccctgtt caaatcattg gaaatgagag agacattcgt tacttttttg
    14881 cacaatattt ttcagaaaaa tattatttcc tagaatggcc atttgaaaat ttttcatcag
    14941 agccactatc tcaattgtta gaattggttt ataaggaaac aagctttcca atgaatttgt
    15001 caacgcatag aatgctaaag ttgctcctag ttacgaacct atatagaata aagtttggtc
    15061 attttatgga agtagataaa gattctttta acgaccaaag tttggatttt ttaatgcagg
    15121 cagaaggaat agaaggtgtt gctcagagtt ttgaatcaga atacaatatc tctttagatg
    15181 aagaagttgt ttgccaatta tttgtgtctt actttcaaaa aatgtttttc atagatgaaa
    15241 gtctctttat gaaatgcgta aaaaaggata gttatgttga aaaatcttac catctattga
    15301 gtgattttat tgatcagatt tcagtcaagt atcagattga aatggagaat aaggataatc
    15361 tgatttggca tctgcataat accgcacatc tgtatcgtca agagttgttt actgagttta
    15421 ttttatttga tcaaaaaggg aatacaatca ggaactttca aaatattttt ccaaaatttg
    15481 tttcagatat aaaaaaagag ctttctcact atttagagac tcttgaggtt tgttctagtt
    15541 caatgatggt aaatcatcta tcttatacct tcatcactca taccaaacat ttggtgatta
    15601 atttgctaca aaatcagcct aaactaaagg ttttggttat gagtaatttt gatcagtatc
    15661 atgcaaaatt cgttgcagag acactttctt attactgtag caataatttt gaactggaag
    15721 tttggactga attagaatta tcaaaggaat ctttagaaga ttcatcttat gatatcatta
    15781 tttctaattt tattattcct cctattgaaa ataagagact catctattca aataatataa
    15841 acacggtctc actcatatat ttgttaaatg ccatgatgtt tattcgatta gatgagtaac
    15901 caaaaataac aaaaaaacgc tatttcgcgt ttgaactgta cctcaaaagt tagacagaaa
    15961 aaatctaatt tttggggtgt ttttgttaag aaaagacctt caatcattta agcaaaatgt
    16021 acatctagat ttatagccct ctaaaactct tgcttttttt gtagaagata cgaaaaaagc
    16081 cataaaacca aggcttttcc tgttttatgt agatgtttac acatgaaaat tgaagtattt
    16141 tcaaaagcta attggagtga ttttattcat gatgggataa atgatctggc caaaaatgat
    16201 tgagaaatag ttgcttaaaa agcatttgca tgatactata tattgaaagg aggtgataat
    16261 atggtagaac aaagaaaatc aattaccatg aaagatgttg ctttagaagc aggagttagt
    16321 gttggaactg tttcacgtgt aattaataaa gaaaaaggca ttaaagaagt aactttaagg
    16381 aaagttcaaa gggctataaa aacattggac tatatccctg ataattatgc tcgaggtatg
    16441 aaaaagaata gaacagaaac tatcgctttg attattccga gcatatggca tccatttttt
    16501 tcggagtttg cctatcatgt tgagaaagag ttattaaaat ataattataa attatttatt
    16561 tgtaacgctg atagcgattc acaaaatgaa atcgaatata tcgaaatgtt gaaacaaaat
    16621 aaagtggatg gaattattgg tattacatat tctgatattg acaaatatat tttatctgat
    16681 ttaccgtttg taagtataga tagacatttt tcggaaaatg tatgctatgt tacttcagca
    16741 aattacaaag gaggacaaac tgcagcagag gaactggtta gaagggataa taagcacctt
    16801 gcttttattg gttcaattaa tatgtatgag aatgagacta tactcagaag taaaggattc
    16861 agagaaaaag ctaaggaatt aggagtagat gtctataatt ttgaaatgaa agaaccttta
    16921 ataaatcctg aagatcagat aagaattttt ttgcaatctc ataaagaaat tgatggtata
    16981 tttacaataa atgatatgat ggctctgaga gttattagtg ttatgaaaga actggggaaa
    17041 atagcacctg atgattacca gataattggg tttgatggtt taaaaacttc tgtcgatcaa
    17101 tcttatctag tgtctacaat agttcaagat attggtatga tggcaaaaga atctgttgca
    17161 atgatactga aaatgattaa gcgagaagag attctagaaa aacgaatcgt aattcccaca
    17221 tattttgggg gagataaaac aacaaaaaaa atttaaaaag cgcttgacaa ttaatgaaag
    17281 cgctgttata atataatcaa caaatgaaac gtttcaaaaa taggctagaa gcttgtttaa
    17341 aaaataaata aaaaatgaaa cgtttcaaaa aggaggaaaa tgatgcgtaa aggtacaatg
    17401 ttatctgttg ttgcggggct atcgttagct atacttgcag gttgtagtgg aggaactaac
    17461 tcaaaacaag cgagttcttc aaatgatatt aaagtctggg tacaattctc tgacgaaaca
    17521 aatgaaggaa aagcttggca aaaagttgtt gataacttta atcaatcagg aaaaagtaag
    17581 tacaaggttg taactgagta tattcctcgt agtggtagtg gtggaggata tgaagacaag
    17641 gttaatgctg ctattacgac taatagtctt ccagatgtta ttacccttga tggaccaaat
    17701 acggcagctt atgcaaaatc taaagtaatt actccgctag atgattatct aaaagataat
    17761 aatatggatg atgttttgga cagtattaaa caacagggaa cttataatgg gaaattctat
    17821 gcatttggat tttctgaatc gaatgttggt gtttattaca ataaaaaaat gttcaaagag
    17881 gctggaattg cggaatcaga gcttccaacg ctagaaaagc catggacatg ggacgaattt
    17941 aatacgattg ctaaaaaatt gaaagatcat tataacaaac cagcaattga ttttcgtatt
    18001 aattcaaacg atgaaatgtt gccatatgct tatatgccgt tgatttggtc aaataatggt
    18061 tctgtcgtaa acgaagatgg aacaaaggca gaaggatact tcaactccaa agagagtgta
    18121 gaagcagttc aattcattca aaatcttgta aaagaaggct atacaactgt tagtccagtc
    18181 gaaaaaggtt ttgaaacagg agagtatcca atgcttttga gtggttcttg gacgattgct
    18241 gacatgaata caaactacaa agatattgat tttggtatcc ttccttaccc agtatctagt
    18301 aagacgaaaa aattagtatc cccttcaggt agttggcaat tagctgtgac gacaaaatct
    18361 gataaaaaag aggccgcatc tgaatttgtt aaatttgcta caaatacaga atcaagtgaa
    18421 attattagct tggggaattc ggtccttcca attcgtaaat ctacaattga aaatatcaaa
    18481 gataaagtat ccgaaccaat gcgtttcttg atggaacaaa attcaaaaac agctcatgct
    18541 cgcccagttg tcgtagcata tcctcaggtt tctcgagctt ttcaacaagc aatgcaagat
    18601 attagctatt atgaagaaaa tccaaatgtg caaaaagtct tggatactcg tacaaaagaa
    18661 atgcaaactg ctattgatca gtcactcaaa taatctgtaa aaaagaaggt ggaaggaaaa
    18721 tgtttggaac taccttcccc tttcttgtta atagcctagt ttgatatata tgattaaagg
    18781 agatggatat gagtaaagaa actgttgtgt atacaaaaag acgtgaaaaa ataaaggata
    18841 acatattagg ttatagtttt ttagctccag ctttaatttt attaggtatc tttttagtaa
    18901 ttccagttgg aatggtcatt tattacgctt ttacagatta ttatttgttg acaccagatg
    18961 aaagaaaatt tgttggactt gaaaatttta ttcgattaac aaaagatccg attttcttaa
    19021 agagcttctt gaatactttg aaatttgttg tttggattat tccagttcaa ttgggagcgg
    19081 ccttaggaat ggctttgatt gtcaataaaa agcgaaaagg aaatatgttt tttaaagtgg
    19141 ctttttttgc tccagttgtc atgtccttgg tagttatttc tattctttgg ctttatcttt
    19201 taaatccaaa tagtggctta ttaaatgcta ttttaaataa ggtaggtatt gcttctcaac
    19261 ctttcttgac tagtcctaaa caagctatgt atgcaattgt ttttgtttct gcttggcagg
    19321 gtgcaggtta tcagatgctt ttatttttag gaggaatgca gaatattcca caagatgtct
    19381 atgaagctgc tgaattagat ggattctcaa aatgggctca gtttcgttat atcacgatgc
    19441 ccctactaaa accaacagcc ctgtttgtat tgttaacaac tttaatttct gcttttaagc
    19501 tgattgtaca accaatggtt atgacgcaag gtggcccatt aaactcaaca attactatgg
    19561 tgtactatat ttatcaacaa ggttttactg atcgtcttgt aggatattct agctcaatcg
    19621 ctttagtatt tacaacgtta attggtatga ttagcttagt acaacgtcgc gttctgaagg
    19681 aggatgacta agatgaaacg atttaaacca acaacaatct tagaatatat tctgttaact
    19741 ttattggcag gactcttcct ttttcctatg gtttggatgg tagtttcagc tatgaagcca
    19801 gaagcggatg tttataaaaa tttgactagt tggcaagcct tcttaccaag tttaaatccc
    19861 gctgattggt ttaaacctta tcaggaagta tttgagcgct ttagtatttt tacatatcta
    19921 gggaacagca ttttttatgc tggaacgttt gctattggtt ctattattgt caatgcactg
    19981 gccggtttcg cttttgcaaa agtcaatttt tcaggtaaga aaattttatt tggtttctta
    20041 cttgctttac ttattattcc agtggaaact gtgttaattc cacaatttac cattatcaat
    20101 tccttaggtt tggtcaataa tcgtcttgca gttattattc caggattggc gagtgtattt
    20161 aatatttact tatttaggaa tttctttatt gcaattccag aagagattct tgagtctgca
    20221 aagatggacg gagcaagtat cgttagaatt ttctttagaa ttatgcttcc aatgtctaaa
    20281 cctgcagttg ctacagttgg tgttttatca tttatttcaa gctggaatga ttatatctgg
    20341 ccattgatgg ttttgacaga ttcttctaaa ttttcaatgc aggttgcaat tacaactatt
    20401 aatacaactc aaccagttta tatcaatcag gtaatggctg tattaactat ttcaacgatt
    20461 ccgttgattt tggtctatgt agtcgctcag aaatatattg ttcaaggtct gggtggttcc
    20521 ggaactggaa taaaatagga tatacttatg ataaataaaa catttacagt agaaaaagcc
    20581 aatcgtttta tagcagaaaa taaacatctc gttaatactc aatataagcc tgaagaacat
    20641 ttttcagctg agattggttg gatcaatgat ccaaatggat ttgtctattt tcgtggagaa
    20701 taccatctct tttatcaatt ctatccatat gatagtgttt gggggcctat gcactgggga
    20761 catgctaaaa gtaaggactt ggtgacttgg gagcacttgc cagtggcact tgctcctgac
    20821 caagattatg accgaaatgg ttgtttctca ggctctgcca ttgtcaagga tgatcgcctc
    20881 tggctcatgt acactggaca tatcgaagaa gaaaccggtg tccgccaagt gcaaaatatg
    20941 gcattttcag atgacgggat tcactttgaa aagatttccc aaaatccagt tgcaactgga
    21001 tcagacttgc cagatgagtt gattgctgct gatttccgtg atccaaaact ctttgaaaaa
    21061 gatggacgct attactccgt agtagctgcc aaacacaagg ataatgtggg ctgtatcgtt
    21121 ctactagggt ccgataacct agtagaatgg cagttcgaat ccatcttttt aaaaggagga
    21181 gaacaccaag gctttatgtg ggaatgccca gattacttcg agttagatgg gaaagattgc
    21241 cttattatgt cacccatgcg ttatcagcgt gagggagact catatcataa catcaactca
    21301 tcgcttttgt tcacgggtaa ggtagattgg agagaaaaac gttttatccc agaatcagtt
    21361 caagaaattg atcatggcca agacttctat gcgcctcaaa cattgttgga cgatcaaaat
    21421 cgtcgtatcc tgattgcttg gatgcagaca tgggggcgta cccttccaac ccatgaccaa
    21481 gaacacaaat gggcatgtgc catgacttta cctagaattc taagattgga agatggcaaa
    21541 ctaagacaat tccctgttaa aaaaggccaa tatcaaatcc aaatagataa agattgtcat
    21601 taccacttag gaaatgatac agattatctt gaatttggtt atgacagtaa tgcgcagcaa
    21661 gtttacattg atcgtagcca tcttattcaa aaaattctag gtgaagaaga acaggacact
    21721 agtcgacggt atgtagatat tgaagctaaa gaattggaag ttgttctaga taaaaattcc
    21781 atcgagattt ttgtcaatca aggtgaagca agcttgactg caacttatta cttaacggtg
    21841 ccagctgagc tatcacgaat tgattaaaaa ttaagttatt tctcctaaag aaaaagttct
    21901 ctttctaaaa tagtggaaag aggacttttt gtgttttggg tatataagct tagtttatgg
    21961 tatttgtaaa attggtgttg gattatgatt taagctagtt ttctaaagaa tttgaaaaaa
    22021 attttattta agcaaaaaaa ccttggttcc aaggcttttc ctgttgtatt tagatgcccc
    22081 ctacagggat cgaacctgtg acccacggat taagagtccg ctgctctgcc agctgagcta
    22141 aggaggcaaa gaaaaagctg tattggtgcc gaaacttcac ggtttgtatt gaacccgcgc
    22201 aattaagcag gtgggcaact cgctctaact gaagctgttt ccgtgtgaga cggcctacat
    22261 gctgttagaa gacttttgtt tccctaataa tacaaaaaat agtcggtcaa cacttaagtg
    22321 tgaagtcgta caccacagcg tttctatact tatatgatac cactttttca aaaaaaatca
    22381 agaggaaagt gcaatttttt gaaaaggatt tcagattttt cgcaaacgat cgatagcttc
    22441 ctttctttga gccaaagccc gttcatattt accagtatct tctgcttgga aatagtgatg
    22501 attacgaatt ttttctggca gatagtcttg cttgatccaa tttccaggat agttgtgtgg
    22561 atagagatag tcttgggcat tccccagttc cttgcttcca ctgtagtgcc catcacgcag
    22621 gtgtcgcgga ataggcaagt gccctgatgt tttgaggtca gcaagtgcct tatccatagc
    22681 tacataggct gagttggatt ttggagaaag ggccaaatca atcacgacat tggcaatgag
    22741 aatgcgggct tcggggaaac caatcctttg tgcagcatcc agagcagtca cggtatgaat
    22801 ctgggcctca ggattggcta agccaatatc ttcataggcg ataacagtca agcgacgagc
    22861 gagactaggc agatccccag cctcaatcaa gcgggcagca tagtggagac tggcatccac
    22921 atctgagcca cggatagact tttgcagggc agatagaaca tcatagtgac cgtctccatc
    22981 cttgtccata gtgatgtagc ttctctgaag actattttcc atgatgtcta aggtgatgtg
    23041 gcgaatgccc tcgtcattct caggggtaga gagaacagcc aagtccagtg agttaaaggc
    23101 agagcgaagg tctccgtttg tagaggttgc gatgaaatcc agcgcatcct catctagttc
    23161 tattggaaaa tcaaaaccac gttcagggtt acttagagct atctgcaggg cctctttgac
    23221 gtcttggtta gacagaggtt ccaactcgaa aatttgaaca cggctacgaa tggcaggagt
    23281 gacagagaag aaaggatttt cagtcgttgc tccaatcatg atgaccagtc cactttccaa
    23341 gagaggcaag aggaagtctt gcttggtctt atctagtcga tgaatttcgt ctagcaatag
    23401 gacgagacca ccagaaaatt tagcttcttc cgagatttct tgcagtcgct ttttactatc
    23461 aactgtcgca ttgaaagttc gaaaggcata cttggtcgtt ccagcgatgg cagaggcaat
    23521 actggttttg ccgattccag gagggccata tagaatcatg gaggacaggc ggttggcttc
    23581 caccatgcgg cggatgattt ttccaggtcc gaccagatgc tcctgaccga tgacctggtc
    23641 gatggtttta gggcgcatgc gaagcgcgag attgtctggc atagcagtcc tttctaacgt
    23701 ggatttgagt agaatgactc atctactata tcttactatg tccattctag cctattagtt
    23761 gtgatatttc aatggttttg taataatcag ggagtgagat agaatctttt ttactattta
    23821 atttaatgag gagaggtatc agcatgagag atttgatttt ccttaaagct caaaatagga
    23881 tagttgagcc taaagaagtg gaagtgctga cttgagaaat aatattgctg aatgtcattg
    23941 tatgaactat aatgaaaaga gaggtgataa gcaatggaaa tgatggacca agtcaggaaa
    24001 ttttgatttt
//
LOCUS       DAVBGY010000026        23784 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVBGY010000026 DAVBGY010000000
VERSION     DAVBGY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23784
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.026"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(84..773)
                     /gene="rplA"
                     /locus_tag="VQ620_001093"
     CDS             complement(84..773)
                     /gene="rplA"
                     /locus_tag="VQ620_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET2763726.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(980..1405)
                     /gene="rplK"
                     /locus_tag="VQ620_001094"
     CDS             complement(980..1405)
                     /gene="rplK"
                     /locus_tag="VQ620_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET2763727.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAVRMIEGTARSMGFTVID"
     gene            complement(1528..2244)
                     /locus_tag="VQ620_001095"
     CDS             complement(1528..2244)
                     /locus_tag="VQ620_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET2763728.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKAKNEDGAAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     VIGTNGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYFGFEGGDYVD"
     gene            complement(2247..2717)
                     /locus_tag="VQ620_001096"
     CDS             complement(2247..2717)
                     /locus_tag="VQ620_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET2763729.1"
                     /translation="MCLICQRIELIKKGENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVSELHHLEKKTRIRFLEEMSVVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     RGDMNGYGLNGRGPVWWIPFEEMTAEICQSKPDEIKRLVERLSSEVDKLLEIKE"
     gene            complement(2719..3756)
                     /locus_tag="VQ620_001097"
     CDS             complement(2719..3756)
                     /locus_tag="VQ620_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET2763730.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVLGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAMLLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTNEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSALVD"
     gene            complement(3854..5179)
                     /gene="brnQ"
                     /locus_tag="VQ620_001098"
     CDS             complement(3854..5179)
                     /gene="brnQ"
                     /locus_tag="VQ620_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HET2763731.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(5350..5442)
                     /locus_tag="VQ620_001099"
     CDS             complement(5350..5442)
                     /locus_tag="VQ620_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2763732.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(5485..5637)
                     /locus_tag="VQ620_001100"
     CDS             complement(5485..5637)
                     /locus_tag="VQ620_001100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2763733.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            complement(5797..6375)
                     /locus_tag="VQ620_001101"
     CDS             complement(5797..6375)
                     /locus_tag="VQ620_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET2763734.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPCT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(6429..7829)
                     /gene="pepV"
                     /locus_tag="VQ620_001102"
     CDS             complement(6429..7829)
                     /gene="pepV"
                     /locus_tag="VQ620_001102"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HET2763735.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLTDLQAKLDAFVAEHKLRGELQEEAGQYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(7842..8447)
                     /locus_tag="VQ620_001103"
     CDS             complement(7842..8447)
                     /locus_tag="VQ620_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET2763736.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDLKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            8618..9418
                     /locus_tag="VQ620_001104"
     CDS             8618..9418
                     /locus_tag="VQ620_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2763737.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(9600..10433)
                     /locus_tag="VQ620_001105"
     CDS             complement(9600..10433)
                     /locus_tag="VQ620_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET2763738.1"
                     /translation="MNHKIAILSDIHGNATALEAVIADAKTQGASEYWLMGDIFLPGS
                     GANDLVALLKELPITASVRGNWNDCVLEALDGQYGLEDPQEIQLMRMTQFLMERMDPA
                     TTVWLRSLPLLEKKEIAGLRFSISHNLPNKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(10423..12270)
                     /gene="uvrC"
                     /locus_tag="VQ620_001106"
     CDS             complement(10423..12270)
                     /gene="uvrC"
                     /locus_tag="VQ620_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET2763739.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGIHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVAAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQRKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            complement(12310..13011)
                     /locus_tag="VQ620_001107"
     CDS             complement(12310..13011)
                     /locus_tag="VQ620_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET2763740.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKMGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(13090..14322)
                     /gene="murN"
                     /locus_tag="VQ620_001108"
     CDS             complement(13090..14322)
                     /gene="murN"
                     /locus_tag="VQ620_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HET2763741.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(14327..15547)
                     /gene="murM"
                     /locus_tag="VQ620_001109"
     CDS             complement(14327..15547)
                     /gene="murM"
                     /locus_tag="VQ620_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HET2763742.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(15659..16438)
                     /locus_tag="VQ620_001110"
     CDS             complement(15659..16438)
                     /locus_tag="VQ620_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HET2763743.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(16507..18168)
                     /locus_tag="VQ620_001111"
     CDS             complement(16507..18168)
                     /locus_tag="VQ620_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET2763744.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPEHIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNVIGDVGNV
                     VLRDRKILSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(18343..20565)
                     /gene="recJ"
                     /locus_tag="VQ620_001112"
     CDS             complement(18343..20565)
                     /gene="recJ"
                     /locus_tag="VQ620_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET2763745.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKTYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            20842..21600
                     /locus_tag="VQ620_001113"
                     /pseudo
     CDS             20842..21600
                     /locus_tag="VQ620_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            21640..22404
                     /locus_tag="VQ620_001114"
     CDS             21640..22404
                     /locus_tag="VQ620_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2763746.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            22417..23094
                     /locus_tag="VQ620_001115"
     CDS             22417..23094
                     /locus_tag="VQ620_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2763747.1"
                     /translation="MIDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILAHIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCMGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            23104..23763
                     /locus_tag="VQ620_001116"
     CDS             23104..23763
                     /locus_tag="VQ620_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2763748.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNTVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLCILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
BASE COUNT         6726 a         5528 c         4390 g         7140 t
ORIGIN      
        1 tcagatatac gacatgcccg tcgtacatca aaaaaagttg agacatagcg tcttcaactt
       61 ttttacatta ctttaactac tgattaaagt gagtttacgt caactttgat accaacacct
      121 tgagtagttg tgatagtcaa gtttgttacg taagttcctt tagctgtagc tggttttgct
      181 ttttggattg tttcgttgaa agctttaaag ttttcaacca atttttcagc ttcaaatgat
      241 actttaccga tgattgcttg aacgttacct gcacggtcag cacggtaagt gattttacca
      301 cctttagact cttcaactgc tttagcaaca tccattgtta cagtaccagt tttagggttt
      361 ggcatcaagt tacgtggtcc aaggacacgt ccaagacgtc caacaagagc catcatatca
      421 ggtgtagcga taactacgtc gaagtccaac caaccgtcgt tgattttagc aacaaggtca
      481 tcttcaccaa caaagtctgc accagcagct tttgcttctt cagcttttgc accacgtgcg
      541 aaaacaagaa cgcgtgaagt tttaccagta ccgtttggca ataccattgc tccacggatt
      601 tgttgatcag cttttttaac gtcgatgttc aagttgtaag caacttctac agttgcgtca
      661 aattttgcaa agtttgtttc ttttgcaagt gctacagctt cttctacgct gtatgctttt
      721 gtgctgtcga ttttctcaag agcagcacga agttgtttgc tttttttagc cattttctat
      781 tctccttgta agtggttcaa tcgattttca tctcccacgt cacttctcga aatgatgaag
      841 tcttgcgggt tttcagtcta ttagattctt ttcctacggc cttaagctcc cttgctgtac
      901 ccaagtacaa gcatcagtca cttgcctagt atgaaaagca actaatagac tgctgtgaga
      961 ttttctactg tgttattgat tagtcaataa cagtgaatcc catagaacga gcagtacctt
     1021 cgatcatacg cactgcagac tctacgtttg ctgcgttcaa atctggcatc ttagtttctg
     1081 caatttcttg tacttgtgca cgagtaactg tagcaacttt agttttatta ggtgtacctg
     1141 atcctttttc aacacctgca gcttttttca aaagaacagc agctggtggt gttttcgtaa
     1201 cgaaagtaaa tgatttatct tcgtaaactg agataacaac tggaatgatc ataccagctt
     1261 ggtcagctgt acgagcgttg aactcttttg tgaatcccat gatgttgata ccagcttgac
     1321 caagagcagg tccaaccggt ggagctggtg tagctttacc agcagggatt tgcaatttta
     1381 caagtttttc gactttttta gccattttta aatcctcctt tgtggttttg gcggtaatta
     1441 gagattttta cctcccacaa gtatgttttt cacataccca tctattatac actatttatc
     1501 tggaattgca agagaaaagt ttatttttta atcgacgtaa tctccacctt caaagccgaa
     1561 atactcttcc aaactgaggc cactagctgc aatctcttta gcctcttttc cgacataacg
     1621 aaggtgccat tcctcagcca tatagcctgt ttccttttcc ttacctttga gataacgtac
     1681 aacaaagcca taatcagccg catgatccaa gagccattgg gccgcttttt cttctgtcac
     1741 caaatcacca tttgtcccaa tcacatcaaa ggctaaacct gtctggtgct cgctatagcc
     1801 aggacgggca gaataacggt cggcagcttc tttcccatct tgattgacat aatcttgata
     1861 gagcttagtc tgagtttcat aacttctaaa gccactgtaa tgatcgctaa tggggaaacc
     1921 tgcctcttgc atagctttga tgagcttgac caactcagcc ttggctgttg gattttcccc
     1981 tggattgtag tcttttgaca atggatagtg tttgttggct acgatgattt catcgtattt
     2041 cccttgaatg ctgtaatagt ctcctttgtt aaccacttct gcttttttct gggcagctcc
     2101 ttgagactta cttcctactg ttccatcagc tttagcagtt tgctctgtct tagctgctcc
     2161 atcttcattt tttgcttttt cttgtgaaca agctgctaga ctcaaggcta gcaaggctgt
     2221 caagacaagg tatctttttt tcatctttac tcctttattt ctaatagttt atctacttct
     2281 gatgataaac gttcgactaa tcttttaatc tcatcaggtt ttgattggca gatttctgct
     2341 gtcatttctt caaagggaat ccaccatact ggaccacgtc cattgaggcc ataaccattc
     2401 atatcacctc ttcgtctagg aaacagatgc caatggagat gagcatcgcc atttcctagt
     2461 agttcgatat tcattttctc agcagcaaag gccttggcaa ctgcctcttg gaccacactc
     2521 atttcttcta gaaaacgtat tctagttttc ttttctaaat gatgcaattc gctgacatgt
     2581 tccttggcta gaaagagact atagcctgca aaatactggt ggtctccaat cacaagatag
     2641 cctgtttcca actctttgac aaaataggga ttttctccct tcttgatgag ctcaattctc
     2701 tggcaaatca aacacatatt agtctaccaa tgcactctta agataggcat cgaccattcg
     2761 ttctgttgcg accaccgagt caatatgagt gcgctcataa gaatgactag actcgatacc
     2821 cgcaccaaga agggcatgct tgacctctgc ccctgcagac atagccgctg aagcatccga
     2881 accataaaat ggatagatgt ccagcttaaa aggaatatct tgctctttag ccaaggcaac
     2941 caagtgttga cggaagtcat agtgataagg acctgaagca tccttgacac agatagatac
     3001 tgtgtactca tttgtctgct ggtcatctcc catagctccc atatccacag ccagatattc
     3061 tactacctga gcaggaatgt tagaattagc accatgtcct acttcttcaa aaactgaaaa
     3121 agcaaaatga gttgttactg gcaattcaat cttctctttc ttataaatac gaagcaggtt
     3181 gagcaacatc gctgcgctga ccttatcatc caaatgacga gacttgataa aacctgtatc
     3241 agtcacaaca gttcgtggat cgaagctgat aaaatcaccg acctcaatcc caagagcacg
     3301 agtttctttt tcattgctta cttttacatc caaacgcact tccatattgt cctgcgtgcg
     3361 ttctgcagtt cctgcatcct tgtagacatg gcaagaagtt tggtggatga ggatggttcc
     3421 taataccttt tgacctgtgc tagccacatg aacggtacag ttttcacctt caatcatgtt
     3481 ccaaggaaaa ccaccgatac ggtccaattt aagacgacca tctggtttga cagcacgaac
     3541 aatagcaccc agtgtgtcca catgggcagt cacatagcga tgttgctcat cattttgacc
     3601 tttgatggtc acattgacac cacccttggc tgtgcgaact ggctggtaac caaaaccttc
     3661 tagagtcttg actaaataat tcgaaatttc acgagtgaag cccgttggcg atgcgatggc
     3721 tgtcagttct ttgatatatt ctactgtttg attcatttct tcacctcttt cgctacctat
     3781 tataacacat ttatcatcgg ataaaaaaag aaaatcactc ctgtagactt ggctacaaaa
     3841 gtgatttaag tgattattcc atttcaaaaa catcgctttc ttgcttgttt ggtagaacca
     3901 atgagagcaa gattccaaca atggctggca ccaaccatgg gagagatgcc ttggcaaaag
     3961 gaagaacgct aacaagattt gcaagaaact caaccttaaa cgagcttcct agtacgcttg
     4021 caatggcaat aactgtaacc acagcaattg tcaactgcat acctggtttt gaaagggcaa
     4081 caaatttgtt gacaatgaca atcataacga tagcaatcgt gattgggtac aagataacca
     4141 gtactggaat tgagtacttg ataatcgcat caagacccaa attggcaata gcaaatccaa
     4201 tcaaggtaaa ggctgtcgca taaaccttgt agctgatttg tgggaagcgc tcattaaaga
     4261 actcagctgt tgacacaatc aaaccaacag tcgttgtgaa gcaggttacg gtaaccatag
     4321 ctgcaaggaa gagttgagct gttgagccaa agatttcttg agtggcttgt gacaagatgt
     4381 aaacacctgg tgttccaccc ttcatcgctt cagctggtac tgggaaatga tttccaagaa
     4441 aacctaaacc gatgtaaaga gcgctgaagg caagggcaac aacgatacca acaacccaaa
     4501 tagttgaaat gtattctttc ttacttgaaa atccaagttg tttcaaggtt tgaactgcga
     4561 ttacgctaaa ggccactgag gcaagggcgt ccaaggtatt ataaccttct aggaaacctg
     4621 taccaaaggc agaagcttga taagcagctg aagcagcttg aggacttgtt ccaccatatt
     4681 tgatagctcc cagaacgacc aagataacaa tcaaaattgc aaagactggc gttaaaatac
     4741 gtccaatgcg gtctaagatt tttgatggat taagcgaaat caaataggct gccgcaaaat
     4801 acagaaccgt aaatacaatc aagccaagtc ctttatttgc atccgacaaa agggggctaa
     4861 tccctacttc gtaagctgtt gtagcagtac gtggggtagc aaagaatgga ccgattgaca
     4921 agtaaagaac tgagaggtaa agagtcgcaa accaaggcgc tatcttcgtt gaaatctcgt
     4981 agatatatcc tttaggattt agcgttccaa taataagggt caagacggcg ataccaacgc
     5041 ctgaaaagac aaaacctgcg atggcaggaa gaaaatgttc tccagataga gcacctagag
     5101 aaggcggaaa aatcaagttc cccgcaccaa aaaatattcc aaacaggagc aaacctgtta
     5161 gggtaccttt tttagccata aaaatctcct tcatatttat aaaatactga tctagtatac
     5221 cacgattagg agtctgaaaa aagcatcacg aggcaaatat taaatgtttt caattttctt
     5281 aaaaataaga attatctaaa aataaaactc tcctacccag tccacaccaa gtaggagaaa
     5341 tgcaaaattt taaagttctt caaaggctaa catgccaccc tggacattga taacattata
     5401 accttgttct aataggaatt ggcaagcacg cgccgatctc attccagatt tgcaaataat
     5461 ataatgcaag cgatctttgt ccaattaatc ataactatca gccaattgac taagcggtag
     5521 gttgtgggca ccttctaaat gaagagctgc aaactcatcc acttctctca catccactag
     5581 agaaagctgg tcgttttggt aaagctggta aaatgcgtca aaggctattt ctttcattct
     5641 ttttcttcta atactcttcg aaaatctctt caaaccacgt cagcgtcgcc ttaccgtgcg
     5701 tatgttaccg acttcgtcag ttttatctgc aacttcaaag ctgtactttg agcagcctac
     5761 gactagcttc ctagtttgct ctttgatttt cattgactat aaaatggttt taattctttt
     5821 tttcaaatct ggcactactt ctgcctcaaa ccaaggattt ttggccatcc agatttgatt
     5881 tcgtggtgat gggtgaacta gcggaaaata ggctggcaga tagtctttat agtgtttcac
     5941 cctctccgtt accttcccac tgattttctc ctgtaaatag taggcttggg catattgccc
     6001 aatcaagagg gttaactgaa tatcaggcaa ttcctgtaag agctgcggat gccatttttc
     6061 tgcaaaacct gtacaaggcg gaagatcacc cgacttgcca tgtcctggaa agtagaaatc
     6121 cataggcaaa acagcaaaat aacctgaatt gtaaaaggta tcttcatcca cacctagcca
     6181 gtcccgcaaa cggtcaccac ttttatcttt ccagtaaagg cctgcttctt gagttttaag
     6241 tcccggagcc tgaccgatga tattgatgcg agcagttttt ggcgctgcaa agagaggctc
     6301 aatgccacgc tctgtatagc tggcattctg cgaatccgcc atgatagcct gtttgattct
     6361 ttcgatttga gacatctact ttccctccaa aaagaagtcc aagcataaga tctcagactt
     6421 ctatcgtttt atttgatcaa ttcgtaaata gcttcggcat aaattgctgc tgctcggaag
     6481 agatcgtcca aggcaataaa ttcattggct tggtgcatgg tatcaattga gtctgggaac
     6541 atagcaccat aggcaactcc gcgctctaac aagcgaccaa aggttccacc accgatgact
     6601 tgttcatgac ctttaaggcc tgtttgtttt tcatagacat tcaacaaggt ttgcacaagt
     6661 ggatcttcca ttggcacata gtgaggggta tgaccatgtt ctgatagact gacagaagca
     6721 actggcaagt tttctaggat tgacttgatt tgttctggac ttgttccttt tggatagcgg
     6781 atgttaaggg caatggtatt atcagcactt gtttcatcga agcggaagac gccggcattc
     6841 atagaaagag cacccatctt ttcatccaca tgagcaatct tgagattttc accctcatga
     6901 tcgttcaaga gaattttacc tgcgatgtca aggtagtctt tggctggacc agcaaagcca
     6961 aactggctga ggaagagggc aaggtaagtt gcgccattga cacctgaagc aggcatagca
     7021 ccgtgggctg atttaccaat gatcgtcacc ttgtattggc cagcttcttc ttggagttct
     7081 cctctaagtt tgtgttctgc aacaaaggca tctagtttag cttgcaagtc tgtcaagtca
     7141 cctgaaacga ctgctgttgc tgattctggt accatatttt cacgtaaacc acctgtaaag
     7201 ctgtgaagac gggctgcacc tgtattttca cctgcaaagt ggaggtattc cgtgatgttt
     7261 cctttttcac cattgatgat tggaaattca gcatctggtg agaaaccaaa gtctggcttg
     7321 gcgagtccta cgtgctcgaa gtagtagtcc atatctgccc agcctgattc ttcgtctgtt
     7381 ccaacgatga agcgaacttt cttagaagtt ggaaggccca attctttgat gattttcaaa
     7441 ccatagtagc aagctgttgt aggtcccttg tcatctgaag caccacgcgc ataaaggcga
     7501 ccatctttga tagttggtgt gtaagggtct gtgtcccaac cgctaccagc tggaaccacg
     7561 tccatatggg caaagattcc gagaacttct tctccatcac caaactcaaa atgtcctgca
     7621 tagttatcaa catttttagt tggataacca tcacggtctg cgatttcaag gaatttttcc
     7681 aaggctttta ctggaccagg tccaaatgga tgttgggcat cagccttgct atcatcacgt
     7741 tctgagttga tttccaaaag gctaaacaag tcagccaaga gatcttcttt gcgtttttct
     7801 acttctgctg taaaatcaat tgctatcatt ttttcttctt cctatctttt ttcgatgatt
     7861 tcatctactg gcaagcggta gcttggttcc aatttgtcgt ctgtgtaacc cactgtgatc
     7921 aagagttctg ggcggaaacg gtcttcaatt tccaaaacct cattgacttt tgatttgtca
     7981 aaaccaagga taatattgga accaattcct tggtctgtaa gagcgagaac caagttcata
     8041 gcaaccaaac ctgcattgag tgctaggtag tcgctgactt gttgttcatt gtagcgtgca
     8101 aattcagctg gcaaattttt catgaagtat tgaagttgtt cttctgagaa attgttagca
     8161 ccaccaactc gggcaatctt acgagcgcgt ttggcaaggt ctgtatctgt aaatagggca
     8221 atggttacag gtgctgatga tacctgctca aagtttgaac cataagccaa ctttgccagt
     8281 tcagcatttt tctcacgaac aactacaaat ttccaaggct ggctgttgtg ggcgcttggc
     8341 gccaaggttg caatttcgat agccgtacgc acatctttta gatcaacagg cttatcagtg
     8401 aaatgcttgg tcgcatgacg ttttttattt aattcaagaa atttcataat cgatttcctt
     8461 ttctaattct actgcttcca ttctaccata atttgaaaga gcttgcaggg atttttcatg
     8521 attaagtttt tctatcacag acataaaaga tatatattgg tttatcagga aagtttctga
     8581 taaactagca agtactttac atttgaatgg agacagtatg aaaaaattta gcctattact
     8641 agctatccta ccatttttgg ttgcctgtgg gaatcaagct acacccaaag agactagcgc
     8701 tcaaaagaca atcgtccttg ctacagctgg cgacgtgcca ccatttgact acgaagataa
     8761 aggcaatctg acaggcttcg atatcgaagt tctaaaggca gtagatgaaa aactcagcga
     8821 ctacgagatt caatttcaaa gaaccgcctg ggaaagtatc ttcccaggac ttgattctgg
     8881 tcactatcag gctgcggcca ataatttgag ttacacaaaa gagcgtgctg aaaaatacct
     8941 ttactcgctt ccgatttcca acaatcccct cgtccttgtc agcaacaaga aaaatccttt
     9001 gacttctctt gaccagatcg ctggtaaaac aacacaagag gataccggaa cttctaacgc
     9061 tcaattcatc aataactgga atcagaaaca cactgataat cccgctacaa ttaatttttc
     9121 tggtgaggat attggtaaac gaatcctaga ccttgctaac ggagagtttg atttcctagt
     9181 ttttgacaag gtatccgttc aaaagattat caaggaccgt ggtttagacc tctcagtcgt
     9241 tgatttacct tctgcagata gccccagcaa ttatatcatt ttctcaagcg accaaaaaga
     9301 gtttaaagag caatttgata aagcactcaa agaactctat caagacggaa cccttgaaaa
     9361 actcagcaat acctatctag gtggttctta cctcccagat caatctcagt tacaataaca
     9421 ctcttcgaaa atctcttcaa accacgtcag ctttatctgc aacctcaaag ctgtgctttg
     9481 agcaacctac ggctagcttc ctagtttgct ctttgatttt cattgagtat aagatatact
     9541 aaaagcgatt ggaccagcca atcgcttttt tagtttgcat tggctatttt aggaaactct
     9601 tacaaaaaat caaaaaaatt cttcacatcc tctacatacc catgcttttc tattaagctg
     9661 gctaaaagtt ccagattgtg cccctgatag ttatcgtcac gacgcagttc ttcatacatt
     9721 tcaataaagg gaagcccctt ggacttggcc aattctaact cagcctcgta atcataagca
     9781 actttacgaa attggatatt gaccaattcc ccatcttcaa cttctatcac ggcatactgg
     9841 gcacggtgat tttttaacgc ctcccaatta aaatagggca tgccaatcga ccctggattg
     9901 atgatttgtt gcccttgact tccataacga agcaactgct tgtgaacatg accataaact
     9961 gccacgtccg tttccgcatc tagcagttgg tcaaatttct ctgtatcatt ctcaactagc
    10021 aagtcaccac catagttctt atttggcaaa ttatgagaga tagaaaagcg cagtccagca
    10081 atttctttct tttccagcaa aggcaagctt cgtagccaga cagtcgttgc aggatccatt
    10141 cgctccatca aaaactgagt cattcgcatg agctggattt cctgtgggtc ttctaagcca
    10201 tattgcccat ctaaagcctc aaggacacaa tcattccaat tgcctcgaac acttgccgtg
    10261 ataggaagtt cctttaacag agcgaccaag tcatttgcac ctgaaccagg gaggaaaatg
    10321 tctcccatga gccaatattc actggctcct tgagttttag catctgcaat cactgcttct
    10381 agcgccgtcg cattgccatg aatatctgat aaaattgcga ttttatggtt tattgtggtt
    10441 tccttccgtt tggtaatcta ctctttcttc cgccacttga agcaagattt ctgtttcctg
    10501 cgggttcaac tttctttgca cagcctctgc tactgctctt ggtactccaa cttcgacaat
    10561 ctcatccaca ctggcttcct tgattttggt cagagacttg aagtgcctca tgagattttg
    10621 cttgcgttta ggtcccagac catcaatccc atccaattga gatgagaaag aatttttgga
    10681 gcgcagttgg cggtggaagg taatggcaaa gcggtggacc tcatcttgaa tgcgttggag
    10741 gaggaaaaat tcctgagaat tgcgagacaa gtccaccact tccagcggat ctccaaagag
    10801 caattcatgg gtttggtgct tatcattctt ttgcagacct gcaattggaa tatccaagcc
    10861 cagttcttct tggatgactt gcttagcaat attgacttga ccttgccccc catcaatcac
    10921 aatcaaatct ggaggagtca aagcctcacg ctgtactcga ccatagcgtc ttcgaatgac
    10981 ttctctcata ctagcatagt cgtccggccc aacaacggtt tttatcttgt acttacggta
    11041 atctttttta cttggcctgc cgttaacaaa gaccaccatg gctgaaacag gactggtccc
    11101 catgatgttg gagttatcga aggactcgat gcgaactgga gtcggaattt ggagcaaacg
    11161 tcctagattt tcaatagctc cttgggtctt ttcgactgat ttttctagca ggttaaattt
    11221 ctgctccaga ctaacacgtg cattttttat ggctagattg accagctgtt ttttctctcc
    11281 acgttggggt ttgagaatct tggtatccac caaagccttg acagcttctt cgtcaatatc
    11341 ctgcggaatc agtacctcat tgggaactag gtgagatttt tcttgataga attgtcccac
    11401 ataggtcaag aaatcctcat ctggatcatt gaagtagggg aagagattga catcgcgctc
    11461 gatgagcttg ccctgacgaa caaagaaaac ctgcacacac atccagccct tatccacata
    11521 gtagccaaag acatcgcgat tttggagatc cttagccatg acccgttgct tggttcgaag
    11581 cgttccaata gcctgaatca ggtcacggta ttccgccgca cgttcaaact ccatactttg
    11641 tgctgctact gccattttac tcttgagatc atcgatgatt ttgtcatcct gacctttcag
    11701 aaaatcagac acctcctgag ccatagactt gaaataagcc tcatccttct tacagatggt
    11761 gtgggccata cactggccga tatggtaata aaaacagacc ttagagggcg ggttggtaca
    11821 ttttcgaaaa gggaaaatcc tatctagaag tcgcttgatt tcattggctg cccccacatc
    11881 tggataaggg ccaaaataaa gaccaccgtc ctttttgacc tgacgagtaa tgataagccg
    11941 tggatagcgc tcattggtga ttttgatgaa aggataggac ttgtcatcct tgagcatgat
    12001 attgtacttg ggcttatttt ccttgatcaa gttgatttcc agcagaagcg cctcaatatt
    12061 ggactcagtg acgataaatt caaaatccac aatttcagac accagagcct ctgtcttggt
    12121 atcatgaatt ccacgaaaat aggaccgtac tcgattacgc agatttttag cctttcctac
    12181 atagataatg gtgccatttt tatccttatg aatgtagcaa ccagggctgg tcggcaagag
    12241 ctctagtttt gatttaatca agttgttcat agttccatta tagcaaaaaa agtagggaaa
    12301 gatagtaccc tactcactag tctcatatat ttttcggagg tttgcaacat cctcttcctg
    12361 gataccataa aaggaacctg ctggttgcat gacagacttc tcatctgtat ggtccaagcc
    12421 aatcgcatga cctaactcat gttctgccgt atggacaagg cgttcatagg agtagccata
    12481 gtatggattg gacaaataat aatgattcaa ccgcaccgtt acggacaaga attgccctgt
    12541 taagagatta gtttgacttt ccgcctctcc tgccacagga gtgcctccgt cgttcatctc
    12601 cgtagccgta atatccgcct tactagactc agtcacgagt tcaaagttaa aagcaccagt
    12661 ttgattccag ttctggattg cttctaaata agcctcttga aagcgtgaat ccatctgcgg
    12721 atccaggtaa atacgagcag aggcctgtgc ccaccttcct tcagaatgct ggtgactatc
    12781 tgtctggctt aacttaggca gttgccctgt tttctcccat tggtcaatac tttgttgacc
    12841 catttttact gaccgttctg cttgctttag cgctccttga aaatccccgt tcagatacca
    12901 gagaagtccg aaagcaagca cacataagag cacaactatc caaaccagac gccaaaacaa
    12961 acgccacaca aaagaaaaga aagcccctat caaacgaaaa agccagcgca tgtctctcca
    13021 cctttctagc aaataagaat tattttacta aaagaagcta aaagaaatct aaatactgac
    13081 tttttcatct taacgtccta ctattttttt gagtaactgg atagctttgt attttaaagg
    13141 cgatggatgg taacggaaag tacctgcttt gcgtacaata tagccattaa aattctgttt
    13201 aaaacgcaaa acaccatcac ttccatcaaa aatcccttga atgcctagga agttgtattt
    13261 aggtattcca cgttttatgc tttccaacat aacatatttt tgaagcagtg caggggcata
    13321 gaacttatta aactcagtgt aggaaccact aaagagataa gtcgtttcct gaggcatata
    13381 aacaaataaa ctcccagcta aaacaatatc ttcttctcca tatttttcaa tcaagtctcg
    13441 cgcttctgct tttcgaactt caaacgtttc aaattgacta gaatattctc tcagttgatt
    13501 ttgttttttc tcagaatgag gatttttact caaatcaagt cgcaacttgt ccaagttttc
    13561 ttctagttta ctttgttcac cttgcaattt gctcatatac tccgaaaaat tcaagcttgc
    13621 tatgagaaac tccgcttgtt ctccaaaagt atcataaaaa tgctcataat attctaaact
    13681 tttatcacta tattctctac gttcagaggt ttcttttgtt atattcttaa aaatcgatag
    13741 ttcttcacgt tttaactttt tcaaccgaat gccaaaggtt tcagcctttt tcaccaaggg
    13801 tttacccttt ttgctaaaac ttttaagcaa actcttttca gttaattcag ttaaatcttt
    13861 atagtataac caatctggtt ctccacctgg gtaacctgtt gttaagccat caaattgata
    13921 acctaaatca gtcaaacctt gaataatact ttttttctca gcatctattg gattaccttg
    13981 gctatcaaaa gtttgataag tttcataagg ttttacaagc aactctaata caccattttg
    14041 cttggcatat tcttttaact ctgcataaaa aactggaaga gcatcttgtt gggtataaat
    14101 cggccccgaa ttgagttcca tatgcagacc acccagcatg ggcaagctat aaaccagagc
    14161 tgcaacttga atttctcctt cttgtttcaa agcaagataa acaattcgag cccctctttt
    14221 ttctagcaaa tcccccatct ggacagattg cataaaggaa cgagaagaaa cctgatcaga
    14281 ataagtctga aactcttctt tcgtgagtgt tgttagtgcc atatacttac tttctatgtt
    14341 tttttcttaa tgttttacgg aaatcaagag caagtcttaa cagaggatag agaggatgag
    14401 tgggcattgt aaattcaccc aagtattctt caatcgttgg attaaatttt tccttaaaat
    14461 gataaagtcc accattgaga gagttttcaa caccacctaa attttgccag accatacctc
    14521 gctcaaaggc atagcgagcc gtttcatacc atgttaaaat tggtgcattg taacgtttaa
    14581 aatcatcatc cataccagca tatatattga cagaggtagt accaaattcc aaactcaaag
    14641 tagccgctaa aggaactctc gcttgaccta catctatata ttcctgcaag aaggtcaatt
    14701 cctctaacaa acgttctttt tccttcttct gcgcttctac ttttgaagtt cgagtcgact
    14761 cagtaaaggt ctcttccaag gctctatttt tcgctaactg ttcttctaac tcttgcgaac
    14821 gtttagaaac atccaaggtg gccaaggtga tataggcctt gtccttaaaa ttatctaaca
    14881 attttttata ataggcttca ttcctcaaat gaatctcttt tcgcttctca gtttttttca
    14941 tcaactccga aaatgaatct aatagttcca gtccaccata ttgaatctca agccctttgt
    15001 ttcgtgctgt tcgaatagcc tgttttgttg acttggaaag tttatcttct tcaaaatttt
    15061 ccttgtatat tttcgcctga atacgaggtt gaatggtgtc tcccatttcc tccgtttttc
    15121 ctgaccacct tactcccatt tgttgcaaac tatcaataat agccagattt tcaggaaatt
    15181 ctgtcttttc ctgattgatt aaactttgag ataggcaaat acttgggtca aaagtcacaa
    15241 aaaccgctct cttactgcga gcataggact taatagactg aatggcaaaa ttcaagagtt
    15301 ctttatcccc ataatccaat ataggtcctc ttgggatgta aaacatttta tagcctagcg
    15361 gaagagttct aatcaaaata ctagctgtcg ccagtaattt ttcttccctg taaacaccaa
    15421 acttctcatg ttgccaatta gacttaactt cctcccaagc actactttgt aatacattgg
    15481 ctaattcatg ttctttgaca aactgatcat attctaatgt gggaatgcca atttgataac
    15541 ggtacatttc ctactctctt tcctccagta ttttattact attctactac tttcgtttga
    15601 aaagtctagt gaaaacatat tttattttag ttctactccc cctatgctga agttcaaact
    15661 aagtcagtct ctcttctaat ttgaaatcaa ttggtagggt tgttaaaaaa acttccaatt
    15721 gtttttccca gtagtaccac tcgtgagttc cagcgctatg gctataggtc acatcaaaac
    15781 ctagtttttt gagatttttc actgcgagat tattggcttc gtacaagaaa tcctgttcgc
    15841 cacaccacgc ccaaagtttg gtctttttat ccgatttttt agccagactt tcaagagaat
    15901 agggactagt tgtccagtct ctaatctctc caaaaacacc tctccagtag gctggacttc
    15961 ccagattttg actttcagga gaaaagtttt gaaagctgag ggcacctgaa aaactagctg
    16021 catgagaaaa acgatttgtc gcaagagcca gtttgaagca gccgtaacct cccatagaaa
    16081 gaccagcgat aaaggtcttt tcacgcttgc tagtcatatt agggaagaag cgtttcagaa
    16141 cctgtggcaa ttcctctgct agagccgtgt agtagtcaaa accatactgg gtatcggtgt
    16201 accaaccatt gctggtattg ggcataacaa cgatgagatt agttcctcga agcaagcgtt
    16261 ctacattggt ccgcttaagc caactattat gatttccaga catcccgtgc aaaaggtaca
    16321 agacgggaat atcttcacac tctggttctt ccactcgatt ggcatcaggg tagaggacat
    16381 tcacccccca ctccatatcc aatacttgtg agtaatactc gattttcatt actgccatga
    16441 ttttctcctt ctatttttct ataagaaaaa gccgaaactc gactttctct ctgtttattt
    16501 caacgattat tttgcttcca tgactactgg taaaatagct ggacgacgct tggtttggtc
    16561 aaagagatac ttggtcaaat tatctcgaac cttcccttta agatctgccc agtcaaagtc
    16621 atctccttga agataatctt ctaccgtttg gttaatcaat tctgaacttt cacggagaat
    16681 atcgcgactc ttcttgagat aaacaaatcc gcgcgtatga acacgagccc tagccacaat
    16741 tttcttctca cgacggttga ctgtaatagc cacgatgaaa attccatcct ctgacaagat
    16801 cttacggtca cgaagaacaa catttccaac atcaccaatg acattcccat caatcaagat
    16861 atctcctgct gaaaccgatc cagctggaac aaagtctcca ttctcgtaag ccatggtcgt
    16921 ccccttttta ggaatgaaga tgtgttctgg caacatccca actgccatgg cagccttagc
    16981 gtgagcatcc aactcacgat actccccttg gacagggaag aggtacttag gttgcaaaag
    17041 attgatcatc agctgcaaat cacgcacatt tccgtgccct gatacatgta aactttgggt
    17101 aatcaatttg acaaccccac ctgcctgata aatcatattt tccacacgcg caacaaaggc
    17161 ttctttagca atagacggag ccgtagcaat atagaccagg tccccatcct tgatttctac
    17221 ataacgatgg cgaccaatcg acatcttacg aagtccattg ataggttcac ccatacgacc
    17281 tgtctcaaga ataatcaact catggtcttc aaagcgagac atatctttag gcttaatcaa
    17341 gagaatttcg ttggctaaag acaacttctt aagacgaatc gctgtgcgga cgatattttc
    17401 aatatcaaat cctgtcaaga cgatacgtcg acctgtttta tccgcagcgt caaaaatctg
    17461 ctgaatacga gaaagattac tggaaacagc tgcaacgatg atacgacctt cccagtcagc
    17521 aatagtttgg gtaatttcat ccctaacttc actttcacta gccacctgaa tattgctgtc
    17581 tgcattggcc gaatcactga ggagagccag gacgccgtca cgaccaatct ctgccaaacg
    17641 agcaaaatca gttgcataag attcactagc cgtttggtca aatttgaagt cacctgtata
    17701 aacgatgctt ccttccgatg tcttcaagac aattcccaga ctctctggaa cggagtaagt
    17761 cgtagggaag aaggaaacca ctgtcccacc aaaatcaatc tccgtattct catcaatgac
    17821 atggaaatca ttaaatttct taacggcatc atttcctttg acaaagagct ttgccaactc
    17881 aatggtcaac tcagacccaa atacaggaac tttagcctct gccaagagat aaggtagagc
    17941 accaatggca tccgcatgcc cgtgggtcaa gaaaacccca gcaatacggt cgctattttc
    18001 aaaaaggtaa tccatgtttg gaatcaccac atcgacccct aattgttcat tttcaggata
    18061 ttttaaccct acattcaaaa caaaaatgga ctctccaatt tcagcaatgt acatattttt
    18121 tccattctca cgcacaccac caagtgttgt taaactgata ttactcattt ttcctccaaa
    18181 atcttagttt atcttgatta tagggcaatt ctaccctctt attctaaaag ttctgatact
    18241 ttcagccgaa tcttttccta ccattatacc atttttttga tgattttcaa aatgaaggtc
    18301 tgttttcaaa aaagagttga tttcccaact ctttcctaac ttctactctt tttccatcaa
    18361 aaaatcataa atttcttgca ccgtacccag cgccatcatt tcttggtctt taacggtttg
    18421 tttgagattt tggtaaattt gactttctcc tatctcccgc tttggcgcct ctttattgac
    18481 tgtcatcaca ccatctttta tcgtcacaaa gcctagttct tcaaatactt gaatcatctt
    18541 gaccagcaag atttgttgaa tattaagata tgcagccaaa tctttcagct tgtagcgaat
    18601 atcaaactct gggaactggt aaatagtctt gtacaattta gcaaactgat ctctagtccc
    18661 ataacctgtc agataatagg tcttgtcaat atcatttttg aaatagacag cagagaaatg
    18721 ctgttcctga aaaatggtct tcagctgagt aatatcctct ggaatgtttt ttacgacaac
    18781 agcttcacta gccgcaagat ttggcagttc tccaggaaaa tccaagactg gaacaccttc
    18841 tggcaagact gcattttttc cacgaatgtt aaaaagttga acaccttcca ctcgcgcatc
    18901 caccatcatc aactggaggg cagtttggcc attccattgg ttgacagaca atttaaccgc
    18961 taactctaga ttcttggttt gagaaaactc tgtcgcccat ctgccttgac caaaggctac
    19021 cacttcaaaa ctcgcctcac ccttggaaat tttcagcttt agatgggcat tacctgcccc
    19081 catagtacga gcactttcga cctgaaaatt cttgatataa aaaataggtt tctgattatc
    19141 cattccaaaa ggagctaaac gttcaaaact tttgaccgtt tccaagctaa gtgcctccaa
    19201 atccaactct tcatctaggt ttaacttatt cttgccacca gcatctgcac ctttttcacg
    19261 aacataatct tccaaaacct gagataaatc tgagagttgc tcaacttcca gcgtcatacc
    19321 cgctgcacct gcatgacctc caaaggcgat gaagaggtct cgatggggat ccagagcttc
    19381 aaaaatatcg accgcttcca cactacgagc actgcccttg gcacgaccgt cttctatatt
    19441 aagaacaatg actgtctgtc ccaattcttc caataaacga ccagccacga ttcctagaac
    19501 cccaggattc cagccttcct tggccaagac ctgaaccttc ttctcaggat ccaccatggt
    19561 cttggcttct tcatagatag actgaacgat ttccttgcgc tcttcgtttt tctggtgaat
    19621 cataagggca atctcatgcg cttcctcatc atcaaatcca gtcaacaaat caatggcagg
    19681 attgggatca tccaagcggc ccaaggcatt caaacgagga gcaatctgga aaccaaccgt
    19741 ttcttctgtt acttcgttgg cagcaatccc agccatgtcc agcatttctt gcagaccaat
    19801 gcgctgggta tgacccaaca tttccagacc atattgaact aagatacgat tttcatccgt
    19861 cagactcacc atatctgcaa tagttccaat agcgaccaaa tcaagcaatt ccacttgcac
    19921 ttcttctaac agggcacaag ccaacttgaa agcaactcca caaccagcca aatatttaaa
    19981 aggataatcc gcatctggat gttcaggatg gacaatagca taagcatctg gcagggtttc
    20041 aggcatggaa tgatggtctg tcacaatgac atctactccc atagactgag ccaatgcaat
    20101 agcctcatga ccagcaaccc cattgtccac cgtcacaatc aaggaaattc cttcttgctc
    20161 gataaagtat ttataaacac tagcattagg gccatagcca tcggtaaaac gatttggcag
    20221 gtaaactcgg cactcagcac caagttgttc caaactttcc ttcacaatag aagccgaagt
    20281 catgccatcc gcatcatagt ctccataaac gagaatattt tccccttctt caatagcctg
    20341 acgaatccgc tccactgcct tgtccatatc atggagcaga taagcatcat gtaagtcctc
    20401 caaggaaggt tctaaaaact tcttcagact ttcttggtcc tgaatccctc tctcaaacaa
    20461 taaccgagcc acctcaggac ccagtccagc cttcttggct atctttgtaa aatccgcatc
    20521 ttctacctgc agggcaaact gccattcata agtaggtgtt atcaaaaaga catccactct
    20581 ttctaagaat tctattgctt cattataaca tgatttagac gttttctcta tccagattgc
    20641 ttttttataa cattttatag tatattgaaa ctagaatagt acacctctac ttctaaaata
    20701 ttgttagaaa tcgatttgac tgtcccgatc gatttgtcct gttcttattt cactatagtg
    20761 aattttttca gatatggttt gacaagaaca ttctttcatt gtagaatcgt gttataaaat
    20821 ctaacacaaa ggagatctct catggcttta gtagaattta aaaacgtcga aaaatattac
    20881 ggagactacc acgcactccg caacatcaat ctccgttttg aaaaaggaca agttgttgtc
    20941 ctgcttggac cttctggctc tgggaagtcc actcttatcc gtacgatcaa tggtttagag
    21001 gctgttgaca aaggaagtct cctagtcaat gggcaccaag ttgctggtgc cagccagaaa
    21061 gatttggtac ctcttcgcaa ggaagtcggc atggtttttc aacattttaa cctttatcca
    21121 cacaaaacgg tgttagaaaa cgtgacactt gcgcccatta aagttctagg aattgataaa
    21181 aaagaagctg aaaaaaccgc ccaaaaatat ctggaatttg taaatatgtg agacaagaaa
    21241 gattcctatc ccgccatgct atctggtgga caaaaacagc ggatcgccat cgctcgtggt
    21301 cttgctatgc atccggaact cctcctcttt gatgaaccaa catctgctct tgatcctgag
    21361 actatcggag atgttctagc agttatgcag aaactggcgc atgatgggat gaacatgatc
    21421 atcgttaccc acgaaatggg ctttgctcga gaggttgcgg accgcattat ctttatggcc
    21481 gacggagaag ttttagtaga tacgacagat gtcgataact tttttgacaa tccaagcgaa
    21541 cctcgtgccc aacaattcct cagcaaaatt atcaaccacg aaagtgacaa agtcaaataa
    21601 ggaggcgcct atgaaaaaaa attcttttta tcagcattat tgattagcct tttcggcctt
    21661 gctgctgcca aaccagtcca ggctgatact agtatcgcag acattcaaaa aagaggcgaa
    21721 ctggttgtcg gtgtcaaaca agacgttccc aattttggtt acaaagatcc caagaccggt
    21781 acttattctg gtatcgaaac cgacttggcc aagatggtag ctgatgaact caaggtcaag
    21841 attcgctatg tgccggttac agcacaaacc cgcggccccc ttctagacaa tgaacaggtc
    21901 gatatggata tcgcgacctt taccatcacg gacgaacgca aaaaactcta caactttacc
    21961 agtccctact acacagacgc ttctggattt ttggtcaata aatctgccaa aatcaaaaag
    22021 attgaggacc taaacggcaa aaccatcgga gtcgcccaag gttctatcac ccaacgccta
    22081 attactgaac tgggtaaaaa gaaaggtctg aagtttaaat tcgtcgaact tggttcctac
    22141 ccagaattga ttacttccct tcacgctcat cgtatcgatg ccttttccgt tgaccgctct
    22201 attctgtctg gctacactag taaacgcaca gaactactag atgatagttt caagccatct
    22261 gactacggta ttgttaccaa gaaatcaaat acagagctca acgactatct tgataacttg
    22321 gttactaaat ggagcaagga tggtagtttg cagaaacttt atgaccgtta caagctcaaa
    22381 ccatctagcc atactgcaga ttaaggagga caccccatga tagatttatc atcttggaca
    22441 gcctattttc aggattttgg acaatttttc aatggtttcc tctttactct tgccctagcg
    22501 attggatcct ttatcctcgc catggtctta ggaatcttct ttggtgccat gtcaaccagt
    22561 aaacgtccaa ttttacgcat tttagctcac atctttgtgg aattttacca aaacactccc
    22621 ctcttggtgc agtttgttat tgttttttat ggcctacctc ttatcagtga ccatactatc
    22681 atgattccaa tttattggac agctgttctc tgcatgggac tctatcacgg cgcttatatc
    22741 gctgaggtta ttcgttcagg gattcagtct attcctagtg gtcagatgga ggccgccttg
    22801 tcgcaaggtt ttacctatat cagtgccatg cgcttgatta tcttgcctca gaccttccgc
    22861 atcattctcc ctccattgac caaccaaatt gttaacctca tcaagaacac ctctactgta
    22921 gctatcatct ctggagtaga cttgatgttt gtgactaagt cttggtcggc tctcaacgga
    22981 aactatatcc cagccttttt aggtgctgct cttctctact ttgctctatg cttccctgtt
    23041 gcccagtttg gtcgcaagat ggagcaagcc aacaaaaaag cctattcact ttaggaggtt
    23101 actatggaat ctattttaga agttttaacc ccagataacc tagtctttat ctttaaagga
    23161 tttggcttga ccctctatat ttctctgatt gccatcatcc tctctactat catcggtacg
    23221 gtgctagctg tcatgagaaa tggcaaaaat cctgtcttac gcattatttc cagtatttat
    23281 atcgagtttg tgcgcaacgt tcccaacctt ctctggattt ttactatctt tttggtgttc
    23341 aaaatgaaat ccacaccagc aggtattaca gcctttactc tctttacatc agcagccttg
    23401 gctgagatta ttcgaggcgg tctcaatacc gtagacaagg gacagtacga agcaggaatg
    23461 tcacaaggct tcacctcagc ccaaatcctc tactacatca ttctcccaca agccatccgc
    23521 aaaatgctac cagccatcat ttctcagttt gttaccgtga ttaaggatac cagtctcctc
    23581 tactctgtta tcgccctaca agaactcttt ggagccagcc aaattctcat gggccgttat
    23641 ttcgaaccag agcaggtctt cagtctttgc atcctgattg ccctcatcta cttcagcttt
    23701 aacctagcaa tttctagcct gtctcatatg ctagccaaac gttggcaaca agctgcagaa
    23761 taatactctt cgaaaatctc ttca
//
LOCUS       DAVBGY010000027        23284 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVBGY010000027 DAVBGY010000000
VERSION     DAVBGY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23284
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.027"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            60..929
                     /locus_tag="VQ620_001117"
     CDS             60..929
                     /locus_tag="VQ620_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET2763749.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            933..1889
                     /locus_tag="VQ620_001118"
     CDS             933..1889
                     /locus_tag="VQ620_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET2763750.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            1889..2653
                     /locus_tag="VQ620_001119"
     CDS             1889..2653
                     /locus_tag="VQ620_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2763751.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            2653..3363
                     /locus_tag="VQ620_001120"
     CDS             2653..3363
                     /locus_tag="VQ620_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2763752.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKIPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            3828..4484
                     /locus_tag="VQ620_001121"
     CDS             3828..4484
                     /locus_tag="VQ620_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2763753.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDESIDLPALREKFEANGIQVEEIARTSAKVL
                     "
     gene            4592..5687
                     /gene="prfB"
                     /locus_tag="VQ620_001122"
     CDS             join(4592..4663,4665..5687)
                     /gene="prfB"
                     /locus_tag="VQ620_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET2763754.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            5705..6397
                     /gene="ftsE"
                     /locus_tag="VQ620_001123"
     CDS             5705..6397
                     /gene="ftsE"
                     /locus_tag="VQ620_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET2763755.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            6390..7316
                     /locus_tag="VQ620_001124"
     CDS             6390..7316
                     /locus_tag="VQ620_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2763756.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMLSPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            7601..9781
                     /locus_tag="VQ620_001125"
     CDS             7601..9781
                     /locus_tag="VQ620_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2763757.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDTSQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKNLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            9833..10648
                     /locus_tag="VQ620_001126"
     CDS             9833..10648
                     /locus_tag="VQ620_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET2763758.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            10788..12131
                     /locus_tag="VQ620_001127"
     CDS             10788..12131
                     /locus_tag="VQ620_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2763759.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKDRQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            12346..13536
                     /locus_tag="VQ620_001128"
     CDS             12346..13536
                     /locus_tag="VQ620_001128"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET2763760.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTDSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            14089..15024
                     /locus_tag="VQ620_001129"
     CDS             14089..15024
                     /locus_tag="VQ620_001129"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HET2763761.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            15058..16095
                     /locus_tag="VQ620_001130"
     CDS             15058..16095
                     /locus_tag="VQ620_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET2763762.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNSSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            16268..16873
                     /locus_tag="VQ620_001131"
     CDS             16268..16873
                     /locus_tag="VQ620_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET2763763.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            17029..17559
                     /locus_tag="VQ620_001132"
     CDS             17029..17559
                     /locus_tag="VQ620_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HET2763764.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            17580..18665
                     /gene="rlmN"
                     /locus_tag="VQ620_001133"
     CDS             17580..18665
                     /gene="rlmN"
                     /locus_tag="VQ620_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET2763765.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQGIVQESADGTVKYLFELPDGMLIETVLMCQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSISGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKSSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGT
                     DIDAACGQLLFNTMKRDRQKAVAAVNP"
     gene            18665..19147
                     /locus_tag="VQ620_001134"
     CDS             18665..19147
                     /locus_tag="VQ620_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET2763766.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVYFCFTPQEQSTVGVGTPDIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            19149..20690
                     /locus_tag="VQ620_001135"
     CDS             19149..20690
                     /locus_tag="VQ620_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2763767.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMEMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DLPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(20746..21549)
                     /locus_tag="VQ620_001136"
     CDS             complement(20746..21549)
                     /locus_tag="VQ620_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET2763768.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQSSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(21632..21853)
                     /locus_tag="VQ620_001137"
     CDS             complement(21632..21853)
                     /locus_tag="VQ620_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763769.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            complement(22332..22541)
                     /locus_tag="VQ620_001138"
     CDS             complement(22332..22541)
                     /locus_tag="VQ620_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763770.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            22711..22983
                     /gene="rpsP"
                     /locus_tag="VQ620_001139"
     CDS             22711..22983
                     /gene="rpsP"
                     /locus_tag="VQ620_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET2763771.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            23003..23242
                     /locus_tag="VQ620_001140"
     CDS             23003..23242
                     /locus_tag="VQ620_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET2763772.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
BASE COUNT         6969 a         4207 c         5105 g         7003 t
ORIGIN      
        1 agaacctatc aaaaagtgag ggaaaaccct cggaattata aatagaaaga gtgaatctta
       61 tgctccaaca actcgtaaat ggtttgattc taggtagtgt ttacgcgctg ttagccctag
      121 gatataccat ggtttacgga attatcaagc tcatcaactt cgcccatggt gatatttata
      181 tgatgggagc ctttatcggt tatttcttga tcaattcttt ccaaatgaat ttctttgtag
      241 cgcttattgt agctatgcta gcgacagcta ttcttggtgt cgtgattgag tttcttgctt
      301 accgaccttt gcgccactct actcgtattg ctgttttgat tacggctatt ggggtttctt
      361 tcctattgga gtatggaatg gtctatctgg ttggtgccaa tacccgtgcc ttccctcaag
      421 cgattcaaac agttcgatat gatttgggac caattagctt aacaaatgtg cagttaatga
      481 ttttggctat ttccttgatt ttgatgattt tgttacaagt cattgtccaa aagactaaga
      541 tggggaaagc catgcgtgca gtatcagtag atagcgacgc ggcgcaattg atggggatca
      601 atgtaaaccg tacgattagc tttaccttcg ctttgggttc tgctcttgcg ggtgcggctg
      661 gtgttctgat tgctctttat tataactctc ttgagccttt gatgggggtt actccaggtc
      721 ttaaatcttt cgttgccgca gtacttggtg gtatcggaat tattcctggt gcggctcttg
      781 gtggctttgt gattggtcta ttggaaacct ttgcgactgc ctttgggatg tcagatttcc
      841 gtgatgccat tgtttatgga atcttgttgt tgatcttgat tgtccgccca gctggtatcc
      901 ttggtaagaa tgtgaaagag aaggtgtaaa cgatgaagga aaatttaaaa gttaatattc
      961 tatggttact ccttttgtta gctggctata gcttgattag tgtactggtt tcagtcggag
     1021 tacttaatct attctatgta cagattttac aacaaattgg aattaatatt attttggctg
     1081 ttggtctcaa cttaatcgtt ggtttttcag gacaattttc acttggtcat gctggtttca
     1141 tggcgattgg tgcctatgca gcagctatta ttggttctaa attaccaacc tacggtgcct
     1201 tctttggagc tatgcttgta ggggctttgc tttcaggagc agttgcctta cttgtcggca
     1261 ttccaacctt gcgcttgaag ggggactatc ttgcggtagc aactctaggt gtttctgaaa
     1321 ttatccgtat ctttatcatc aatggtggaa gtcttacaaa tggtgcggca ggtatcttag
     1381 ggattcctaa ctttacaact tggcaaatgg tttacttctt tgtcgtgatt acaaccattg
     1441 caaccttgaa cttcttgcgt agcccaattg gccgttcaac cctctctgtt cgtgaagatg
     1501 aaatcgctgc tgagtcagtt ggggttaata cgactaaaat taaaatcatc gcttttgtct
     1561 ttggtgccat tactgcaagt attgctgggt cacttcaggc aggatttatc gggtctgttg
     1621 taccgaaaga ttacaccttc attaactcaa tcaacgtttt gattattgtt gtatttggtg
     1681 gactcggttc cattacaggt gcgattgttt cggctattgt tctgggaatt ttgaatatgc
     1741 ttctccaaga tgttgctagt gtgcgtatga ttatttacgc tttagccttg gtattggtaa
     1801 tgattttcag accaggtgga ctccttggaa cgtgggaact gagcctatca cgtttcttta
     1861 aaaaatctaa gaaggaggaa caaaactaat ggcattactt gaagtaaaac agttaaccaa
     1921 acattttggt ggtctaacag ctgttggaga tgtgactctt gaattgaacg aaggggaact
     1981 ggttggatta atcggtccaa acggagctgg gaaaaccacc cttttcaacc ttttgaccgg
     2041 tgtttatgaa ccaagcgagg gaacagtaac cctagatggt caccttttga atgggaaatc
     2101 accttataag attgcctctt tgggacttgg acgtactttc caaaatatcc gtctctttaa
     2161 agatttaaca gttttagata atgttttgat tgcttttgga aaccatcaca aacagcatgt
     2221 ttttactagt ttcttacgct taccagcttt ttacaagagt gaaaaagaat taaaggctaa
     2281 agctttggaa ttgttgaaaa tctttgattt agatggtgat gcagagactc ttgctaaaaa
     2341 tctttcctac ggacaacaac gtcgtttgga aattgttcgt gcccttgcta cggaacctaa
     2401 aattctcttc ttagatgaac cagcagcagg tatgaaccca caggaaacag ccgaattgac
     2461 tgagttaatt cgtcgtatca aagatgaatt taagattaca atcatgttga ttgaacacga
     2521 tatgaatctg gtcatggaag taacagaacg tatctacgta cttgaatatg gccgtttaat
     2581 tgctcaagga actccagacg aaattaagac caataaacgc gttatcgaag cttatctagg
     2641 aggtgaagcc taatgtctat gttaaaagtt gaaaatcttt ctgtgcatta cggtatgatc
     2701 caagcagtcc gtgatgtaag ctttgaagtt aatgaaggag aagttgtttc ccttatcggt
     2761 gccaacggtg caggtaagac aactattctt cgcaccttgt caggtttggt tcgaccaagt
     2821 tcaggaaaga ttgaattttt aggtcaagaa atccaaaaaa taccagctca gaaaatcgtg
     2881 gcaggtggtc tttcacaagt tccagaagga cgccacgtct ttcctggctt gactgttatg
     2941 gaaaatcttg aaatgggagc tttcttaaag aaaaatcgtg aagaaaatca agctaacttg
     3001 aagaaggttt tctcacgctt tcctcgtctt gaagaacgta agaaccaaga tgcagctact
     3061 ctttcaggag gggaacaaca aatgcttgcc atgggacgcg ctcttatgtc aacaccaaaa
     3121 cttcttcttt tagatgaacc atcaatggga cttgccccaa tcttcatcca agagattttt
     3181 gatatcattc aagatattca gaagcaagga acaaccgtcc tcttgattga acaaaatgcc
     3241 aataaagcac ttgcaatctc tgaccgagga tatgtattgg aaacaggaaa aatcgtccta
     3301 tcaggaacag gaaaagaact cgcttcatca gaagaagtca gaaaagcata tctaggtggc
     3361 taaaacaatc cagtggattg ttttagtcgg cagatgaaga ttacgaagta atcatcaata
     3421 tagtccgggg gcccttttta gtcggtagat tgagattgcg aagcaatcat caaatccaga
     3481 ggattgtttt agccggcaga taaagattac gaagtaatca tcaatatagt ccgggggccc
     3541 tttttagtcg gtggatgaag attgcgaagc aatcatcaaa tccagaggat tgttttagcc
     3601 ggtagattga gattgcaaac aaatctgcat ctacattgaa agcttaattt ctaataattg
     3661 aaaaaatcga atgaaaagtt tcttaccttc attcacagag ctcgatttta gagctctttt
     3721 tgctagctta ttcatacttt tctaaatttt gaaaaagaaa tgtaagcgtt tgatagattt
     3781 gcaaaaagat tgtataatag ggataagaat agaaaaggag aagtctcatg gcagttaaag
     3841 attttatgac ccgcaaggta gtttatatta gtccagatac aacagtatct catgcagcag
     3901 atttgatgag agagcaaggt ttgcaccgtc tgcctgttat cgaaaatgat caattggttg
     3961 gtttggtgac tgagggaacc attgcacaag caagtccatc taaagcaaca agtctttcta
     4021 tctatgagat gaattatctt ctgaataaga caaaagtaaa agatgtcatg attcgcgatg
     4081 ttgtcactgt ctcaggctat gctagtctag aagatgcaac ttatctgatg ttgaaaaata
     4141 agattggtat tctccctgtc gtagataacc atcaagtata cggagttatt actgaccgtg
     4201 acgttttcca agcctttctt gaaattgcag gttatggcga agaagggatt cgtgtacgct
     4261 ttgttacaga agatgaagtt ggtgttcttg gaaaaattgt ttctttgatt gtagaagaaa
     4321 atttgaatat ctcccataca gtcaatattc cgcgtaagga tggtaaggtg attatcgaag
     4381 tgcaaatcga tgaatcaatt gatttaccag ccttgagaga aaaatttgaa gcaaatggta
     4441 ttcaagtgga agaaatcgct cgcacttcag caaaagtctt gtaagaaggg aagtccaaag
     4501 gcttcttttt tcatgaaaag gggattagag caaaagatgg aaagaaatga taaaatatgc
     4561 tataatgaaa taatgtaaaa aaggagtatt tatggacatt tcagtaattc gtcagaaaat
     4621 tgacgcaaat cgtgaaaaat tagcttcttt cagggggtct ctttgacctc gaagggctag
     4681 aggaagagat tgccatcttg gaaaacaaga tgacagaacc tgatttttgg aacgataata
     4741 ttgcggccca aaaaacgtcg caagaattga atgaattaaa aaacacttac aataccttcc
     4801 ataagatgga agagttgcag gatgaagtcg aaattttatt ggattttttg gctgaagacg
     4861 agtcagtgca tgatgaactg gtagcgcagt tagccgaact tgataagata atgaccagct
     4921 acgagatgac tctactcttg tcagaacctt atgaccacaa caatgccatc ttggaaatcc
     4981 atccaggttc tggtggtact gaggcgcagg actggggtga tatgttgctt cgtatgtata
     5041 ctcgttatgg taatgctaaa ggctttaaag tggaagtgtt ggattaccaa gcaggtgatg
     5101 aggctggtat taagtcggta actttatcat ttgaagggcc taatgcctat ggtctcctca
     5161 agtcagaaat gggtgttcac cgcttagtgc gaatctcacc atttgactct gccaaacgtc
     5221 gccatacctc tttcacatct gtagaagtga tgccagaatt ggatgatact attgaagtgg
     5281 aaatccgtga agatgatatc aagatggata ccttccgttc aggtggtgcc ggtggacaaa
     5341 acgtcaataa ggtttcaaca ggtgtacgtt taacccacat tccaactgga attgttgtcc
     5401 aatcaacagt ggatcgtacc cagtatggaa atagagatcg tgccatgaag atgttgcagg
     5461 ctaagctcta tcaaatggag caagataaga aggctgcgga ggtagattct ctcaaaggtg
     5521 agaaaaagga gatcacttgg ggaagccaaa tccgttctta tgtcttcacg ccttatacta
     5581 tggtaaaaga tcaccgaact agctttgagg ttgctcaggt agataaggtt atggatgggg
     5641 acctagatgg ttttatagat gcttatctca agtggcgaat tagctaagat agaaaggaac
     5701 tcacatgtca attattgaaa tgagagatgt cgttaaaaaa tacgacaacg gaacgactgc
     5761 tctacgcggt gtttcggtca gcgttcaacc gggggaattt gcttacatcg taggaccttc
     5821 aggagcaggg aagtcaactt ttattcgttc tctgtatcgt gaagtaaaaa tcgataaagg
     5881 aagtctatca gttgctggtt ttaatctggt taagatcaaa aagaaagatg tcccgcttct
     5941 acgtcgtagt gttggggttg tcttccaaga ttataaattg ttaccaaaga aaactgtcta
     6001 tgaaaatatt gcttacgcta tggaagtaat cggggaaaat cgccgtaata tcaaaagacg
     6061 agtgatggaa gttttggact tggttggatt gaagcataag gttcgttctt tcccaaatga
     6121 actctcaggt ggggagcagc agcgaattgc gattgcgcgt gcaattgtaa ataatcccaa
     6181 agtattgata gctgatgagc caacaggaaa tctggatccg gataattcat gggaaattat
     6241 gaatctcttg gaacggatta acctacaagg gacaactatt ttgatggcga ctcataatag
     6301 ccagattgta aataccttgc gccaccgtgt cattgccatt gaaaatggcc gtgtcgttcg
     6361 tgacgaatca aaaggagagt atggatacga tgattagtag attttttcgc catttatttg
     6421 aagccttaaa aagtttgaaa cgaaatggtt ggatgacagt agctgctgtc agttcagtca
     6481 tgattacttt gaccttggtg gcaatatttg catctgttat tttcaataca gcgaaactag
     6541 ctacagatat tgaaaataat gtccgtgtag tagtttatat ccgaaaggat gtggaagata
     6601 atagtcagac aattgaaaaa gaaggtcaaa ctgttacaaa taatgactac cacaaggtat
     6661 atgattcttt gaagaacatg tctacggtta aaagtgttac cttttcaagt aaagaagaac
     6721 aatatgaaaa attaaccgag ataatgggag ataactggaa aatctttgaa ggagatgcca
     6781 atcctctcta tgatgcctat attgtagagg caaacactcc aaatgatgta aaaactatag
     6841 ccgaagatgc taaaaaaatt gaaggtgtct ctgaggttca agatggcggt gccaatacag
     6901 aaagactctt caagttagct tcatttatcc gtgtttgggg actagggatt gctgctttgt
     6961 taatttttat cgcagttttc ttgatttcaa ataccattcg tattaccatt atttcccgca
     7021 gtcgcgaaat tcaaatcatg cgcttggttg gagctaaaaa cagttatatc cgtggaccgt
     7081 tcttgttaga aggagccttt atcggtttat tgggagctat cgcaccatct gttttggtct
     7141 ttattgttta tcaaattgtt taccaatctg tcaacaaatc gttggtaggg caaaatctat
     7201 ccatgcttag tccagattta tttagtccgt tgatgattgc cctactattt gtgattgggg
     7261 ttttcattgg ttcattggga tcaggaatat ccatgcgccg attcttgaag atttaggtaa
     7321 aatagctgct tttatgagga gattgtaaaa tctccttttt tgctacaaga gtttttgaaa
     7381 agagatgcgt agaagaaaag agcttccaaa gaagtccccc agagaagact tcttgatggc
     7441 taatttgaac ttgaaatttg gcaactagaa attttttaga aaaaagtgta atttttttat
     7501 aaaagccttt acaaaaaaaa ttaaagtggt ataattaaat catagaaggt gcaaacgttt
     7561 tcgtaaaacg tttgcctaaa taaaaataaa ggagatttga atgatgaaag atacattcaa
     7621 aaatgtcttg tctttcgaat tttggcaaaa attcggtaag gctttgatgg tagttatcgc
     7681 ggttatgccg gctgctggtt tgatgatttc aatcggtaag tctatcgtga tgattaaccc
     7741 aacctttaca ccacttgtca tcacaggtgg aattcttgag caaatcggtt ggggggttat
     7801 cggtaacctt cacattttgt ttgccctagc cattggagga agctgggcta aagaacgtgc
     7861 tggtggtgct ttcgccgctg gtcttgcctt catcttgatt aaccgtatca ctggtacaat
     7921 ttttggtgta tcaggcgata tgttgaaaaa tccagatgct atggtaacta ctttctttgg
     7981 tggttcaatc aaagttgctg attactttat cagtgttctt gaagctccag ccttgaacat
     8041 gggggtattc gtagggatta tctcaggttt tgtaggggca actgcttaca acaaatacta
     8101 caacttccgt aaacttcctg atgcactttc attcttcaac gggaaacgtt tcgtaccatt
     8161 tgtagttatt cttcgttcag caatcgctgc aattctactt gctgctttct ggccagtagt
     8221 tcaaacaggt atcaataact tcggtatctg gattgccaac tcacaagaaa ctgctccaat
     8281 tcttgcacca ttcttgtatg gtactttgga acgtttgctc ttgccatttg gtcttcacca
     8341 catgttgact atcccaatga actacacagc tcttggtggt acttatgaca ttttaactgg
     8401 tgcagctaaa ggtactcaag tattcggtca agacccacta tggcttgcat gggtaacaga
     8461 ccttgtaaac cttaaaggta ctgatactag tcaatatcaa cacttgttag atacagtaca
     8521 tccagctcgt ttcaaagttg gacaaatgat cggttcattc ggtatcttga tgggtgtgat
     8581 tgttgctatc taccgtaatg ttgatgctga caagaaacat aaatacaaag gtatgatgat
     8641 tgcaacagct cttgcaacat tcttgacagg ggttactgaa ccaatcgaat acatgttcat
     8701 gttcatcgca acacctatgt atcttgttta ctcacttgtt caaggtgctg ccttcgctat
     8761 ggctgacgtc gtaaacctac gtatgcactc attcggttca atcgagttct tgactcgtac
     8821 acctattgca atcagtgctg gtattggtat ggatatcgtt aacttcgttt gggtaactgt
     8881 tctctttgct gtaatcatgt actttatcgc aaacttcatg attcaaaaat tcaactacgc
     8941 aactccaggg cgcaacggaa actacgaaac tgctgaaggt tcagaagaaa ccagcagcga
     9001 agtgaaagtt gcagcaggct ctcaagctgt aaacattatc aaccttcttg gtggacgtgt
     9061 aaacatcgtt gatgttgatg catgtatgac tcgtcttcgt gtaactgtta aagatgcaga
     9121 taaagtagga aatgcagagc aatggaaagc agaaggagct atgggtcttg tgatgaaagg
     9181 acaaggggtt caagctatct acggtccaaa agctgacatt ttgaaatctg atatccaaga
     9241 tatccttgat tcaggtgaaa tcattcctga aactcttcca agccaaatga ctgaagcaca
     9301 acaaaacact gttcacttca aaaatcttac tgaggaagtt tactcagtag cagacggtca
     9361 agttgttggt ttggaacaag taaaggatcc agtatttgct caaaaaatga tgggtgatgg
     9421 atttgcagta gaacctgcaa atggaaacat tgtatctcca gtttcaggta ctgtgtcaag
     9481 catcttccca acaaaacatg cttttggtat tgtgacggaa gcaggtcttg aagtattggt
     9541 tcacattggt ttggacacag taagtcttga aggtaaacca tttacagttc atgttgctga
     9601 aggacaaaaa gttgcagcag gagatctcct tgtcacagct gacttggatg ctatccgtgc
     9661 agcaggacgt gaaacttcaa cagtagttgt cttcacaaat ggtgatgcaa ttaaatcagt
     9721 taaattagaa aaaacaggtt ctcttgcagc taaaacagca gttgctaaag tagaattgta
     9781 atatacttga ggttggaagc tgtattccaa cctcttattt tgggagaaaa gaatgaaatt
     9841 tttaacactc aatactcaca gttggatgga gaaagaagca gaggaaaaat tccagatttt
     9901 gcttgaagat attcttgaaa aggactatga tttgatttgt ttccaagaaa tcaatcagga
     9961 gatgacctcg tcagaggtgg aggttaatga cctttatcaa gctttgccag cagctgagcc
    10021 tattcaccaa gaccattatg ttagactctt ggttgaaaag ttgttagagc aagggaaaaa
    10081 ttactactgg acctgggctt ataaccatat cggctatgac cgctaccacg aaggtgtggc
    10141 tatcttgtct aaaacaccta ttgaagccag agaaattttg gtttcagatg tggatgatcc
    10201 aacagactat catactcgcc gtgttgccct agctgaaact gtagtcgatg gcaaggagct
    10261 agcagttgcc agtgttcatc tctcttggtg ggataaaggt ttccaagaag aatgggcacg
    10321 atttgaggct gtcttgaaaa aattgaacaa gccactttta ctagctggag attttaacaa
    10381 cccagcaggt caggaaggtt accaagctat tttagctagt ccattaggct tacaagacgc
    10441 atttgaagtt gctcaagaga aaagtggtag ctatactgtt ccgcctgaaa ttgatggctg
    10501 gaaagggaac actgaacccc ttcgaatcga ttatgtcttt actaccaaag agttagcggt
    10561 ggaaaattta catgtcgtat ttgatggtaa caagagtcca caagtgagtg atcactatgg
    10621 cttgaatgct atgttaaact ggaaataata actgaaaaga ggttggaact ataaaattcc
    10681 agccttttct tactagagaa gctactggaa atagcctaaa taagtgagac tactgtaatg
    10741 gaataaaata tggtataatt gataagatag atagaatcga ggatgttatg tcatttacga
    10801 aatttcaatt taaaaactat attagagaag ccttgaagga gttaaaattt acaactccaa
    10861 cagaggtgca agacaagttg attcctattg ttttggcagg tcgtgaccta gtaggagaat
    10921 caaaaacagg ttcaggtaag actcatactt tcttgttacc gattttccag caattagatg
    10981 aagctagcga tagtgtgcaa gcagtgatta ctgcaccgag tcgtgagttg gctactcaaa
    11041 tttaccaagt agcgcgtcag atttcagctc actcagatgt cgaagttcgt gtggttaatt
    11101 atgtgggtgg tacggataag gatcgccaga ttgagaaatt ggcaagcaat cagcctcata
    11161 ttgttattgg aacaccaggc cgtatctacg acttggttaa atctggtgat ttagctattc
    11221 ataaagcaaa gacatttgtt gttgatgaag cagatatgac cttggatatg ggattcttgg
    11281 aaactgttga taagattgct ggcagtcttc caaaagactt gcaattcatg gtcttctcag
    11341 cgactatccc acaaaaactg caaccattct tgaaaaaata cttatcaaat cctgttatgg
    11401 agaaaattaa gaccaaaacg gttatttctg acaccattga taattggttg atttcgacca
    11461 agggacatga taagaatgct caaatttacc agttgactca gttgatgcag ccgtatttgg
    11521 caatgatttt tgttaacact aaaacgcgtg ctgatgaatt gcattcatat ctgactgctc
    11581 aaggcttgaa ggttgcaaaa atccatggag atattgcccc tcgtgaacgc aagcgaatca
    11641 tgaatcaggt gcaaaatctg gattttgagt atattgtcgc aacagatttg gcagcgcgtg
    11701 ggattgacat tgaaggtgtc agccatgtca tcaatgatgc cattccgcaa gacttatctt
    11761 tttttgttca tcgtgttggt cgtactggac gaaatggcct accaggtaca gctattaccc
    11821 tttatcagcc aagtgatgac tcggatatcc gtgagttgga gaaattggga atcaagttta
    11881 gtcctaagat ggtcaaagac ggggaatttc aagataccta tgaccgtgat cgtcgtgcca
    11941 accgtgagaa aaaacaagat aaacttgata tcgaaatgat tggtttggtt aaaaagaaaa
    12001 agaaaaaagt caaaccgggt tataagaaga aaattcaatg ggcggttgat gaaaagcgcc
    12061 gtaaaaccaa gcgtgctgaa aatcgcgctc gcggtcgtgc agagcgtaaa gctaaacgcc
    12121 aaacatttta atagaaattg ttggagtatt gagctccaac ttttttattt atgagaacga
    12181 actatctaaa ccgaaacact acattaaaga ctgcaaattg cgattaaaaa tggtataatg
    12241 ataaagttat atagtcccga taagatggta ggtatttatt acgaagagtt ttcctatcag
    12301 tactttgtaa ctctataaca atatttttta aggggggaca tttttatgtc agaacgtaaa
    12361 ttattcacgt ctgaatctgt atctgagggg catccggata agattgcaga ccaaatttca
    12421 gatgcgattt tggatgctat tttagcaaag gatccagagg cgcacgttgc tgctgaaaca
    12481 gctgtatata ctgattctgt ccatgttttt ggtgaaattt ctacaaatgc ctatgtggat
    12541 attaaccgtg tggttcgtga taccattgca gagattggtt ataccaatac agaatatgga
    12601 ttttctgctg agacggtggg agtacaccca tctttggtgg aacaatctcc tgacatcgct
    12661 caaggtgtta acgaagcctt ggaggttcgt ggaaatgctg atcaagatcc actggacttg
    12721 attggagcag gtgaccaagg gctcatgttt ggatttgcag tagatgaaac agaagagctc
    12781 atgccattgc caattgcact cagtcataaa ttggttcgtc gtctggcaga acttcgtaag
    12841 tctggagaaa ttagctatct ccgtccagat gcaaaatcac aagttacagt tgagtacgat
    12901 gaaaatgacc gtccggtacg tgtagataca gtcgttattt ctactcagca tgatccagag
    12961 gccactaatg aacaaatcca tcaagatgtg attgacaagg tcatcaaaga agttattcca
    13021 tcttcttatc ttgatgataa gacaaaattc tttatcaatc cgacaggtcg ttttgtaatc
    13081 ggtggtcctc aaggggactc aggtttgact ggtcgtaaga ttattgtaga tacttatggt
    13141 ggctactctc gtcatggtgg tggtgccttc tctggtaaag atgcgactaa ggtggatcgt
    13201 tcagcctctt atgcggctcg ctatattgcc aagaatatcg ttgcagcagg ccttgctaag
    13261 aaggcagaag tgcagttggc ctatgctatc ggtgttgcgc aacctgtttc tgttcgtatc
    13321 gatactttcg gtacaggaac agtagctgaa agtcaacttg aaaaagcggc tcgtcaaatc
    13381 tttgaccttc gccctgcagg gattatccaa atgctggacc tcaagcgtcc aatttaccgt
    13441 caaacatcgg cttacggtca catgggacgt acagatattg atcttccatg ggaacgtttg
    13501 gataaggtag atgctttgaa agaagcagta aaataagatt ttaagagggg aacgtcctct
    13561 cttttttata gtttttaact atactgggat actgttctga aaatccattt tgcgaaagta
    13621 gagatttaca tgtataatgg attgaaacta gaatagtaca cctcaacttc taaaacattg
    13681 ttagaaatcg atttgactgt cctgatcgat ttctcctgtt cttgtttcat tttactatat
    13741 ttctttaaaa atgataaagg ttgagatttc tcctcgtaat agataatcat ggggatattt
    13801 caatccaaag ttttattcgt tatcacttga ctattgcaag gttttctaga gcaacagagt
    13861 catggaatgg actcatggtt gagatttctc cttgttgctt ggacttcatt caaaagtctg
    13921 ttacccaagc cttgttcaaa cttccaatac actagctgtt tccatagcat gacttctgta
    13981 ctagactttc ttttccgaat aaatagatag aaccacagaa tctagtaaac ctagaattaa
    14041 aattatggta taatattagc aataaaagaa atctggagga ttagaatcat ggtatcaacg
    14101 aaaacacaaa ttgctggttt tgagtttgac aattgcttga tgaatgcagc aggtgtggct
    14161 tgtatgacga tagaggagtt agaagaggtc aaaaactcag cggcaggaac ctttgttact
    14221 aagacagcga ccttggactt ccgtcagggg aatcctgagc cacgctacca agatgttcca
    14281 cttggttcca tcaactctat gggcttgcca aataatggct tagactatta tttggattat
    14341 cttttagatt tgcaggaaaa agagtcgaac cgaactttct tcttatctct ggtcggcatg
    14401 tctccagagg aaacccatac tattttgaaa aaagtccaag agagtgattt tcgtggtctg
    14461 actgagctaa atctttcctg tccaaatgtt ccaggtaaac ctcagattgc ctatgatttt
    14521 gagacaacag accggatttt ggcagaagtg tttgcttact tcaccaaacc tcttggaatt
    14581 aaattgccac cttattttga tattgttcac tttgaccaag cggcagctat tttcaacaaa
    14641 tatccgctca agtttgtcaa ctgtgttaac tctatcggaa acggcctcta tatagaagac
    14701 gaatctgtcg ttattcggcc taagaatggt tttggtggaa ttggtggaga atacatcaaa
    14761 ccgactgctc tagccaatgt tcacgccttt tatcaacgtt taaatcctca aatccaaatt
    14821 atcggaacag gtggcgttct gactggtcga gatgcctttg aacacatcct ctgtggagca
    14881 agtatggtgc aggtgggaac gacccttcac aaagaaggcg tcagtgcttt tgaccgcatt
    14941 accaatgaac tgaaagcaat catggtggaa aaaggctacg agagcttaga agatttccgt
    15001 gggaaattgc gctatattga ctaaattaaa tcgaaaaatc tgaagaaagg agagacgatg
    15061 ctagccattg aagaaagtca gaagttgact ttatcaaatt taccgagcct gagcctattt
    15121 acagggacag atcagggtca gtttgaagtg atgaagagtc aagtgttgaa acagattggg
    15181 tatgattctg ctgacctcaa ctttgcctac tttgatatga aagaagtagt ttacaaggat
    15241 gtggaactgg agttggtcag ccttcctttc tttgcggatg agaaaatcgt gatattagat
    15301 tattttatgg atatcacgac tgctaagaaa cgctttttga cagatgatga gcttaagtca
    15361 tttgaggaat accttgacaa ttcttctcca acaaccaagt tgataatctt tgcagaagga
    15421 aagctggata gcaaaagacg gttagtcaaa ttacttaagc gtgatgccaa ggtcttcgat
    15481 gcagtagaag taaaagaaca agaattgcgc cagtatttcc aaaagtggag tcagaaacaa
    15541 ggtctgcagt ttaccaatca ttcttttgaa aatctcctca tcaagtcggg gtttcaattt
    15601 agcgaaatcc agaaaaatct tctcttttta cagtcctata aggcgaattc tgttattgag
    15661 gaagaggata ttgttaacgc aattcccaag accttgcagg acaatatttt tgatttaact
    15721 cagtttattc tgactaaaaa gatggatcag gcgcgcgatt tggtgagaga cttgaccttg
    15781 caaggggaag atgaaatcaa actgattgca gtcatgctgg gacaatttcg gacttttact
    15841 caggtgaaga ttttggcgga gtctggccaa acagaatcgc agattgcaag tagtttaggt
    15901 agttatctgg gacgtaaccc aaatccttat caaatcaagt ttgcattaag agattcgaga
    15961 ggactttctt tgagctttct gaagcaagct atttcctatt tgattgagac agactatcag
    16021 attaagacag gtctttatga aaaaggtttc ctttttgaaa aggcactctt acagattgct
    16081 agtcaggtca attgacattt gttgaaacta caacccgcgt caggattatc ttgtcgtggg
    16141 tttcttgctg atttgcttat tttattagaa agtatgtcag tgatttcctt atctataagc
    16201 aaaaaacttg aaaaaagtga atgtttgcgt tatcattttc atatagaaag aaaaagaggt
    16261 attacagatg gctattatct taccagaact tccatatgca tacgacgctt tggaaccata
    16321 catcgatgcg gaaacaatgc atttgcacca tgacaaacac catcaaactt atgtcaacaa
    16381 tgccaatgca gctttagaaa aacaccctga aatcggtgaa gaccttgaag ccttgcttgc
    16441 tgatgtagaa tctatcccag ctgatatccg tcaagcactt atcaacaatg gtggcggaca
    16501 cttgaaccac gctcttttct gggaattgat gactcccgag aaaacagctc cttcagcaga
    16561 actggcagca gcaatcgatg caacatttgg ttcatttgaa gaattccaag cagccttcac
    16621 tgcagcagca acaactcgtt ttggttcagg ttgggcatgg ttggttgtca acaaagaagg
    16681 gaaacttgaa gtgacttcaa cagcaaacca agacacacca atctcagaag gtaaaaaacc
    16741 aatcttgggc ttggacgttt gggaacatgc ttactacgtg aaataccgca acgtgcgtcc
    16801 tgactacatc aaagctttct tttcagtaat caactggaat aaagtagatg aattgtacgc
    16861 agctgctaaa taatgatagt tggagggaag aattgttctt ctctttttag gttataagat
    16921 tctggtctga caaaatcgtc agactttttt catttttatg agaaacgtgc taactgctag
    16981 aaattatggt agaataaagt attaagtaat cgtggaaaaa ggatatctat gcgaaaagaa
    17041 attgcacctg aattatacaa ctataacaag tttcctggtc cggagttcca tttacacggg
    17101 gataaggtcg aaacggaagg gatagctttt tccttggttg aaaatatcaa ggatgccttt
    17161 gatgtgacga cttttaatca gcgtttttca gaagtcttaa ccaagtttga ttatatcgtg
    17221 ggggactgga gcaacgaaca gcttcgccta cgaggttttt acaaggatga ccgaacagaa
    17281 gaaaaacttg aaaaaatcag tcgtttacaa gactaccttt tagagtattg tagttatggt
    17341 tgtgcctatt ttgtcttaga aaatgaagcc cctaagcgag catcatttga caagaaaatg
    17401 cgtaagacgg aagaagaaac accttctaga aaaggaaaga aaccgactca aactaaacga
    17461 aagtcgaatg cagataagaa aaatagacgt cgtcagaaag accagcattc tcagaaagag
    17521 gacaaggaac aacgtcattt tgtcattcgt cagaagtgag tagagaataa ggagaagaaa
    17581 tgaaaccctc aattcatagt ttggcacatc aaaccatgca ggaatgggta ttggagcagg
    17641 gagaaaagaa attccgtgca gatcaaatct gggaatggct ctaccgtaaa cgtgtgcagt
    17701 catttgaaga aatgaccaac ctttccaagg atttgattgc taagctcaat gaccagtttg
    17761 tggtcaatcc cttgaaacaa ggtattgtgc aagagtctgc tgatggtaca gtcaaatatc
    17821 tctttgaatt gcctgacggt atgttaattg agactgtact catgtgtcaa cactatggtt
    17881 tatctgtctg tgtgaccact caggtcggct gtaatatcgg ttgtaccttc tgtgcctctg
    17941 gtttgattaa gaagcagcgt gacctcaata acggtgaaat cgtagcgcaa atcatgctgg
    18001 ttcagaaata ctttgatgag cgtggtcagg atgaacgcat cagccatatc gttgtcatgg
    18061 gaatcggtga gccctttgat aattacaaca atgtcttgaa tttctttcgt actatcaatg
    18121 atgataaagg aatggctatc ggtgctcgtc acatcacggt ttcaatctca ggtttggctc
    18181 ataaaattcg tgactttgct gatgaagggg ttcaggtcaa tcttgccgtg tctcttcacg
    18241 cacccaacaa tgaacttcgt tcaagtatca tgaagattaa ccgtgccttt ccgattgaaa
    18301 aactctttgc tgctattgaa tactacattg agacaacaaa ccgtcgtgta acctttgaat
    18361 atatcatgct taatgaagtc aacgacggtg tggaacaagc cttggaattg gctgaattgc
    18421 tcaagaacat caagaaatcg tcttatgtaa acttgattcc ttataaccca gttagtgagc
    18481 atgaccaata tagccgtagt cccaaagagc gcgtgttggc tttctatgat acgcttaaga
    18541 aaaaaggggg caactgtgtg gttcgtcaag agcatggtac agatattgac gcagcttgtg
    18601 gacaattgct ctttaacaca atgaaacgtg accgccagaa ggcagtcgca gcggtaaatc
    18661 cttaatgatg aagaagactt ataatcatat tttggtctgg ggagtcattt tctatagcat
    18721 ttgcattgtc tatttttgct ttactcctca agaacaatct accgtgggag tgggaactcc
    18781 agatattcag catcttggac gcctggtttt tcttttgact cctttcaatt ctctctggaa
    18841 actgggcgaa gtgagtgaca ttggacaatt atgttggatt tttttacaaa atatcctcaa
    18901 tgtcttcttg ttttttcctc tgattttcca actcctttat ctatttccaa atttgcggaa
    18961 aacaaaaaag gtccttcttt ttagttttct tgtgagtctt ggaatcgagt gtacgcaatt
    19021 aatcttggac tttttctttg atttcaatcg cgtctttgag attgatgatt tgtggaccaa
    19081 cactttgggt ggctatctgg cttggctcct ttataaacga ttacataaaa acaagataag
    19141 gaattaaaat gagtatttta gaagttaaaa atctgagtca cggttttggt gaccgtgcaa
    19201 tttttgaaga tgtgtccttc cgtctcctca agggagaaca tatcggcctg gtcggtgcca
    19261 atggtgaagg aaaatcaacc tttatgagta tcgtgactgg taaaatgctg ccagatgaag
    19321 gaaaggttga gtggtccaaa tatgtgacgg ctggttactt ggatcagcac tctgtccttg
    19381 ctgaaagaca gtcggtgcgt gatgttctcc gtacggcttt tgatgagctt ttcaaagctg
    19441 aagctcgtat caatgacctc tatatggaaa tggctgaaga cggtgcggat gttgatgctc
    19501 tcatggaaga agtaggagaa cttcaagacc gtctggagag tcgtgatttc tataccttgg
    19561 atgctaagat tgacgaagta gcgcgtgctc ttggtgttat ggactttggc atggatacgg
    19621 atgtaacttc tttgtcaggt gggcaaagaa ccaaggtgct tttggcaaaa cttctccttg
    19681 aaaagcctga tatcttgctg ttggacgagc cgaccaacta cttggatgct gagcatattg
    19741 attggctcaa gcgctatctc caaaactatg agaatgcctt tgttctcatt tcgcacgata
    19801 ttccattcct caatgacgtt atcaatattg tctatcatgt ggaaaatcaa cagctgacgc
    19861 gttactctgg tgactactac cagttccaag aagtttatgc tatgaagaaa tctcagctag
    19921 aggcagccta cgaacgccag cagaaagaga ttgcagacct caaggacttt gtggctcgta
    19981 ataaagcccg tgttgcaact cgtaatatgg ctatgtctcg tcaaaagaaa ttggataaga
    20041 tggatattat cgaactccaa agtgagaaac caaaaccatc ctttgatttc aaaccagctc
    20101 gtacaccagg gcgctttatc ttccaagcca agaacttgca aattggttac gaccttcctc
    20161 ttactaagcc tttaaatctt accttcgaac gcaatcaaaa ggttgcgatt attggtgcta
    20221 atggtattgg aaaaacaact ctcttgaaga gtctcttggg cattatctcg ccaatcgctg
    20281 gggaagtgga gcgtggagat tatttagaac ttggttattt tgagcaggaa gtagaaggcg
    20341 gtaatcgcca aactcctctt gaagctgtct ggaatgcctt tcctgccctt aatcaagcag
    20401 aagtccgtgc agcccttgcc cgttgtggtt tgacaaccaa acatattgaa agccagattc
    20461 aagtattatc agggggagag caagccaagg ttcgtttctg tctcttgatg aatcgtgaaa
    20521 acaacgtttt agtgctggac gagccgacca accatttgga tgtggatgca aaggatgagc
    20581 tcaaacgcgc tctcaaagaa tataggggat ctatccttat ggtctgccac gagccagact
    20641 tttatgaagg ctggatagac caaatatggg attttaataa tttaacttaa gaatgagagt
    20701 aaaagaaata cagtaccgaa tgtggatact gtattttttt ggtttttaag atttaaaatc
    20761 gtagtctttc accacttcga tgctgtcgat tgtgatagca gtagttggct tgtctttttc
    20821 atctttttcg gccttagcaa tcttatccac aacatccata ccgtcaatca cttgaccaaa
    20881 gactgggtgt ttgccatcta gactagggtt tccaccttct ttgtaggctt cgataatttt
    20941 ctgtggatac ttgcttgtag ggagtttaga agaggtatct gtagagcttt ggttgatgaa
    21001 gaactggctg ccattggtgt ttggttgacc agtattagcc atagcaagag caccacggat
    21061 gttatacaaa taaggagtaa tctcgttctt gaaaccagtt cctttgtctt tagtcttatc
    21121 cttgtcatgc cagatggact gaccacctgt accgtcccct tttggatctc cagtttggac
    21181 cataaagcca tcgatgacac ggtggaaggt aataccgtta tagtagcctt ctttggcgtg
    21241 agtgaggaaa ttttcaaccg ctagaggagc gagtttaggg aagagtttaa tgcgaatatc
    21301 accttggctt gtgtggagaa tcacttcggc ttcatcttca gcaacttcct tagatagttg
    21361 tgggaaattg gcgttttcgt ttgttaaagc atcgtttaac tccttggcag attgggcagc
    21421 tactttggaa ctttcttcag cagcaagact ggaatcaaca taatcatcac cacgcagact
    21481 gcgttggacg ctgctacacc cagctagggc tacagtagac agtaaaagaa gggttgctag
    21541 ttttttcatt ctgatcctct caaaaattca tttataccat tgtaccctaa aaagaagtgg
    21601 atttcaaata ttagtagttt acagaactgg cttagataat agttgggatt tcatcatcat
    21661 cttgaaaatc ttcaacttct tcaacttctt ccccagtaaa tggtttctga aaagaactta
    21721 gtataaaacg ggcaattggt tcaacaatta ggaaatttag aggaagagct gcaataaagt
    21781 ttacgaacca ggcgcttaga taggtattgg gtgtgatagc aatgccattg taaaaaagtc
    21841 catagaagga catgaaagtg accagctaat tcttcttctg aaaaatgctc atgaagggct
    21901 tgatccaggt cgcaagattt cattttagaa agtccagtaa ctcccttctt cttcaggata
    21961 ttctttttca tggtattatt tagatggtca agtgagtcaa aagaagtttc gacaagagca
    22021 tacccttttt ctaccagagt ttttagatgt ttaggggcat caataccata agtgtactca
    22081 aagtattttg gaaaccagat cttaagaaag ctcgtaaagc tagccagttc tctaaacgct
    22141 aagaactagc tacaacacag catttacaac acttcatggt tcacaccatg gggtgctttt
    22201 taatgatttt aagcaaaatt cacacttaat tgaaagttat ttaaattaga tgaaaattta
    22261 ttttttgaaa aagcgggaaa acattgattc taagcgaatt ttgaatcata ttcgaatggg
    22321 agtttttaaa atcatgtttc agtggtaaaa gtaccaaact ggattctcca tagtaaaatg
    22381 atatctcctg ctagtgaatc atctactaat aagtccatat ttcgcttggg aacgggcttg
    22441 ggacttagaa gccaagtatc caactctcgt tcaggcgata tgaagggttt gacttggtgt
    22501 tctttataaa atttttctaa aatagtattc acggatagca tctttcaaac atttatttcc
    22561 tattctaaca aatttctaaa tactttaaga aatttatagt ctgtcaagtt tttttcttga
    22621 cacatgccag aaatctgcta taatagaaca tgtgctaaat agctcagcta tttcaccgaa
    22681 ataaaaaatt aaagaaatga gactttaaaa atggcagtta aaatccgttt gactcgtatg
    22741 ggttctaaga aaaaaccttt ctaccgtatc aacgtagcag attcacgttc accacgtgac
    22801 ggacgtttca tcgaaacagt tggaacttac aacccacttg ttgctgaaaa ccaagtaact
    22861 ttgaaagaag accgcgttct tgcatggttg gctaatggag ctcaaccttc agacacagta
    22921 cgtaacatcc tttcaaaaga aggcgtattg aaaaaattcc acgattctaa attttcaaaa
    22981 taagtttaaa gtaggttgac agatggatac gattgaaaat ctcattattg cgattgtgaa
    23041 acccttaatt tcacaaccag atgccttaac tatcaagatt gaggatacac cagaattttt
    23101 ggaatatcat ttgaatcttg atcaaagcga tgtgggtcgt gtaatcggtc gtaagggtcg
    23161 cactatttct gcgataagaa cgattgtcta ctctgtccca actgaataca aaaaagtaag
    23221 aatcgttatt gacgaaaaat aagaagggcg ggacggatgt ctcgcttttt tgtgtggaat
    23281 agaa
//
LOCUS       DAVBGY010000028        22680 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVBGY010000028 DAVBGY010000000
VERSION     DAVBGY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22680
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.028"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(<1..215)
                     /locus_tag="VQ620_001141"
     CDS             complement(<1..215)
                     /locus_tag="VQ620_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HET2763773.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAARGLQ
                     LGIKTGTWQGAATGAAGGAILGGVAYAA"
     gene            complement(284..553)
                     /gene="blpJ"
                     /locus_tag="VQ620_001142"
     CDS             complement(284..553)
                     /gene="blpJ"
                     /locus_tag="VQ620_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HET2763774.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            complement(1020..1217)
                     /gene="blpI"
                     /locus_tag="VQ620_001143"
     CDS             complement(1020..1217)
                     /gene="blpI"
                     /locus_tag="VQ620_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HET2763775.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            1499..2086
                     /locus_tag="VQ620_001144"
     CDS             1499..2086
                     /locus_tag="VQ620_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HET2763776.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            2079..3668
                     /locus_tag="VQ620_001145"
                     /pseudo
     CDS             2079..3668
                     /locus_tag="VQ620_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
     gene            3679..5040
                     /locus_tag="VQ620_001146"
     CDS             3679..5040
                     /locus_tag="VQ620_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HET2763777.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMDESQKTAL
                     ETQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLANSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LIASQGQTQGTAEEPFLSQINQSIASLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLLLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     SIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            5097..5252
                     /gene="blpC"
                     /locus_tag="VQ620_001147"
     CDS             5097..5252
                     /gene="blpC"
                     /locus_tag="VQ620_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HET2763778.1"
                     /translation="MDKKQNLTSFQELTTTELNQIIGGGWWEDFLYRFNIIEQKNTKG
                     FHQPIQL"
     gene            complement(5296..6636)
                     /locus_tag="VQ620_001148"
     CDS             complement(5296..6636)
                     /locus_tag="VQ620_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2763779.1"
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGISLTFERIFKAFLFKILL
                     AVVFGMLGYMVGNTYLSYFMEPLYGIGLSFLLLRELPKKLLLFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDDYSFIWLCIIIFNFFISLAFLKWLDYDFTSLRKGILDKDFQK
                     SLTQINWIMGAYYLVIQNLSYFEYEQGIQSTTVRHLILVFYLLFFMGIIKKLDTYLKD
                     KLHERLNQEQDLRYREMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLKDSSEKLQDNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTVVSILCDNAIEASVEACQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQDHVFRQVLTVIHIE"
     gene            complement(6650..7387)
                     /locus_tag="VQ620_001149"
     CDS             complement(6650..7387)
                     /locus_tag="VQ620_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2763780.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKEHHITPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSTRPHRVILYTKTDRLEFTASLEEVLKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(7392..7730)
                     /locus_tag="VQ620_001150"
     CDS             complement(7392..7730)
                     /locus_tag="VQ620_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2763781.1"
                     /translation="MKHMIIQTQKTVYKVNVDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQYGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            8007..8336
                     /locus_tag="VQ620_001151"
     CDS             8007..8336
                     /locus_tag="VQ620_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET2763782.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(8504..9553)
                     /locus_tag="VQ620_001152"
     CDS             complement(8504..9553)
                     /locus_tag="VQ620_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2763783.1"
                     /translation="MKDLFLKRKQAFRKECLCYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEAIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(9550..10281)
                     /locus_tag="VQ620_001153"
     CDS             complement(9550..10281)
                     /locus_tag="VQ620_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2763784.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            10349..10759
                     /locus_tag="VQ620_001154"
     CDS             10349..10759
                     /locus_tag="VQ620_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET2763785.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMNATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            10769..11056
                     /locus_tag="VQ620_001155"
     CDS             10769..11056
                     /locus_tag="VQ620_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763786.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            11218..11457
                     /locus_tag="VQ620_001156"
     CDS             11218..11457
                     /locus_tag="VQ620_001156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763787.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSSKHCF
                     EVVDRTDEVSNHTHDKPTLTWFEEIFEEYHSPFHN"
     gene            complement(11807..12943)
                     /gene="dnaJ"
                     /locus_tag="VQ620_001157"
     CDS             complement(11807..12943)
                     /gene="dnaJ"
                     /locus_tag="VQ620_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET2763788.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(13148..13501)
                     /locus_tag="VQ620_001158"
     CDS             complement(13148..13501)
                     /locus_tag="VQ620_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763789.1"
                     /translation="MSVNKNNLSDIADYHEKIKFFEELFNLDYNPTEMLVTVERNGNV
                     IYKEFTEVAVGKSKYDHTSVFKDVMIYFSDHICQELHLYKTQDPHYNKFEFDLAKRSL
                     KITTRDNTTIFISNE"
     gene            complement(13514..15337)
                     /gene="dnaK"
                     /locus_tag="VQ620_001159"
     CDS             complement(13514..15337)
                     /gene="dnaK"
                     /locus_tag="VQ620_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET2763790.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(15817..16341)
                     /gene="grpE"
                     /locus_tag="VQ620_001160"
     CDS             complement(15817..16341)
                     /gene="grpE"
                     /locus_tag="VQ620_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET2763791.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(16368..17402)
                     /gene="hrcA"
                     /locus_tag="VQ620_001161"
     CDS             complement(16368..17402)
                     /gene="hrcA"
                     /locus_tag="VQ620_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET2763792.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTGLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLEAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(17566..18075)
                     /locus_tag="VQ620_001162"
     CDS             complement(17566..18075)
                     /locus_tag="VQ620_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HET2763793.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            18682..21015
                     /locus_tag="VQ620_001163"
     CDS             18682..21015
                     /locus_tag="VQ620_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET2763794.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVSEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            21028..22491
                     /locus_tag="VQ620_001164"
     CDS             21028..22491
                     /locus_tag="VQ620_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HET2763795.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            22491..>22680
                     /locus_tag="VQ620_001165"
     CDS             22491..>22680
                     /locus_tag="VQ620_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HET2763796.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDD"
BASE COUNT         7199 a         4640 c         4130 g         6711 t
ORIGIN      
        1 gctgcatagg ctacacctcc aagtatagct ccacccgcag caccagttgc tgcaccttgc
       61 catgttcctg ttttaatgcc tagttgaaga cctcttgctg ctgctcctcc aacacctgct
      121 ttggcaaaat ctccccaatt gcatccgcca ccttcaacgc aagcaagcat ttcagtatcc
      181 ataacagaaa attgtgacat catttttgta tccatgacaa atactccttt tttaaaaaac
      241 taaaataaat cagaatagaa tcctcataat tttactataa gtcttaccaa cttagtccca
      301 atttatcacc aaccatacct cctaagcatg ttaatccacc cccaattgca ccaatgtgtg
      361 ctccaacaaa tgcaccagca agtccagcta ctcctaaagt ggccaaacct gctccagttc
      421 caccagttat aattcccgta gtgactcctg taatcagtgc attttgacaa tcagtggagc
      481 tatacccccc ttcaactttc gcaagcattt cagtatccat aacctctaac tgtgacaaca
      541 tttttgtatt catgatgaat acctcctttt tattttcaat ttgttaccaa agtcttaaat
      601 tcaataaaca aatagatttt ttatagtatc tttttgattt tcttaaaaaa gtatatacgt
      661 ctactatctt cttaaaggta gcagtaccta ttttttagtc taagatttca ataatcttga
      721 gtatctaaaa tatcttaatt tcgttattct ccttgcaata aaaagtttta ctatactatt
      781 tattaacttg cagaaagcaa aaaatattag taaataatag tttatagtta agttttttat
      841 tcctaccaat ccatcaacta agtaaagcat caacgattac ataaacgatt gataatataa
      901 ttaaaatttt gctaactatc ttattctcat cattcttaga taactttgat attttgtaag
      961 taagtaaata agacagtaaa ttaatagcga taataatact atatttaaga atcataatct
     1021 tacaaagagg acataattcc tgaacctaca caaataagtg ttgctgctcc cccagttatc
     1081 ggaccagtcg cagcagctaa tagtactgct ccaatacaac caccgattgc agatcctaaa
     1141 ttgcctcttc ctccactaac tatttcgagt tcttcattat ccataacaga aaattgttcc
     1201 atcatttttg tattcatgac aaatactcct tttttctttt tttatttttg tcttgttgta
     1261 actttgataa gtttagtata tcatcgtttt ttaaaatttt tcatccagat cttgaattgt
     1321 catcgaaacg tcttgaatta gcttttttat ttcaagccac ctctaaatgt ttaaaaaaaa
     1381 taatttctaa tcactttttt accattcagg aagttttaat gactattcaa gatttcataa
     1441 aatatgaact tagttttatg acataataga cctatccact atatgaaaag aattgccaat
     1501 gacttcttat aaacgtacat ttgttcctca aatagatgcg agagactgtg gtgtcgctgc
     1561 cttagcctcg attgctaaat tctatggttc agatttttct ctagctcact tgagagaact
     1621 tgcaaagacc aataaagaag ggacgactgc tcttggcatt gtaaaagccg ctgatgaaat
     1681 gggctttgaa acaagacctg ttcaagcaga taaaacgctc tttgacatga gtgatgtccc
     1741 ctatccattt atcgttcacg ttaacaaaga aggaaaactc caacattact atgttgtcta
     1801 tcaaacaaag aaagactatc tgattattgg tgatcctgac ccttctgtaa aaatcactaa
     1861 aatgtcaaaa gaacgctttt tctatgaatg gactggagta gctatttttc tagctaccaa
     1921 acccagctat caaccccata aagataaaaa gaatggtcta ctaagcaagc ttccttcctc
     1981 tgattttcaa acaaaaatct ctcattgctt acattgttct ctcaagctta ttggtcacta
     2041 ttatcaatat aggtggttct tactatctcc aaggaatctt ggatgaatac attccaaatc
     2101 agatgaaatc aactttagga atcatctcag ttggtctggt tatcacctag gttatcacct
     2161 atatcctcca acaagtcatg agcttctcca gagattatct cctaaccgtt ctgagccaga
     2221 gattaagcat tgatgtgatt ttatcctata ttcgccatat ttttgaactt cccatgtctt
     2281 tctttgcgac acgtcgtaca ggagaaatca tttcacggtt cacagatgct aactctatta
     2341 tagatgcctt ggcttctacc attctttctc tttttctgga tgtttctatt ctgattcttg
     2401 tagggggagt cttgctggca caaaacccta atctcttcct cctttctctt ctttccattc
     2461 ctatatacat gttcatcatc ttttctttta tgaaaccttt tgaaaaaatg aaccatgatg
     2521 tcatgcaaag taattctatg gttagctctg ccattatcga agatatcaac gggattgaaa
     2581 ctataaagtc gctcacgagt gaagaaaatc gctatcaaaa tatagacagc gaatttgtag
     2641 attatttgga aaaatccttt aagctcagta aatattctat tttacaaacg agtttaaagc
     2701 agggaacaaa attagttctg aatatcctta tcctatggtt tggcgctcaa ttagtcatgt
     2761 cgagtaaaat ttctatcggt cagctgatta cctttaacac acttttttct tactttacaa
     2821 ctcctatgga aaatattatc aacctccaaa ccaaactccc atctgcgaag gtcgctaata
     2881 accgtttgaa cgaagtctat ctagtcgaat ctgaatttca agttcaagaa aaccctgtcc
     2941 attcacattt tttgatgggc gatattgaat ttgatgacct ttcttataag tatggttttg
     3001 gacgagatac cttaacagat attaatctca cgattaaaca aggagataag gttagcctag
     3061 ttggagttag tggttctggt aaaacaactt tagccaaaat gattgtcaat ttctttgaac
     3121 cctacaaagg gcatatttcc atcaatcatc aggatattaa aaacattgat aaaaaagtct
     3181 tgcgccgtca tattaattac ctaccccaac aagcctatat ctttaatggc tctatcttgg
     3241 aaaatttaac cttgggcggt aatcatatga ttagccaaga agatattcta agagcttgtg
     3301 aattagctga aatccgtcaa gacattgaaa gaatgcctat gggctatcaa actcagctct
     3361 ctgatggagc tggtctatca ggaggacaga agcaacgaat agccctcgct cgtgctcttt
     3421 taactaaatc tcctgtttta atactagacg aagctactag cggtcttgat gtcttgactg
     3481 agaaaaaagt tatagataat cttatgtccc taactgataa aaccattctc tttgtagccc
     3541 atcgtctcag tatagccgaa cgaactaacc gtgtcattgt tcttgaccag gggaaaatca
     3601 ttgaagttgg tagtcaccaa gagttaatgc aggcgcaagg cttctaccat catctgttca
     3661 ataaataagg agaatgtcat gaatcctaat ctttttagaa gcgtcgagtt ttatcagaga
     3721 cgttaccata actatgcgac agtgttaatt atacctcttt cattactgtt tactttcatc
     3781 ttgattttct cccttgttgc cacaaaagaa attactgtta cttcccaagg agaaatcgcc
     3841 cctacaagtg tcattgcatc cattcagtca accagtgata atcctatcct tgctaatcat
     3901 ttagtggcaa atcaagtagt tgaaaaaggt gacttactca tcaaatactc tgaaacaatg
     3961 gatgagagtc agaaaactgc cttagaaact caattacaaa gacttgagaa gcaaaaagaa
     4021 ggacttggaa ttttgaaaca aagtttagaa aaagcgactg atcttttttc tggcgaggat
     4081 gagtttggct accataatac ctttatgaat tttactaaac aatcccatga tattgaactg
     4141 ggtatcacaa agactaacac cgaagtttca aatcaagcta atcttgccaa tagcagttca
     4201 tcagccatcg aacaagaaat tacaaaagtt caacaacaaa ttggagaata tcaagagttg
     4261 agagatgcta tcataaataa cagagcacgc ttaccaactg gcaatccgca ccagtcaatt
     4321 ttaaatcgtt atcttatagc ctctcaagga caaacacaag gaacggcaga ggagccattt
     4381 ttatctcaaa ttaatcaaag tattgcaagt cttgaatcat ctatcgcaag cctcaaaatt
     4441 cagcaagctg gtatcggaag tgtagcaact tatgataata gtttagcaac caaaattgaa
     4501 gtactccgca ctcagttttt acaaacagcc tcacagcaac aactaaccgt ggagaatcaa
     4561 ttaacagaat taaaagtaca actagatcaa gcgacacagc gcttagaaaa taatacctta
     4621 accgctccaa gtaaaggtat cgttcatctg aacagcgaat ttgaaggtaa aaatagaatt
     4681 ccaactggta cagaaattgc tcaaatattc cctgtcatca cagatacaag agaagtacta
     4741 atcacttact acgtatcatc tgactatctc ctcttactag ataaaggaca aactgtaaga
     4801 ttaaaactgg agaagattgg aaatcacggc accaccatca tcggccaact tcagagtatt
     4861 gatcaaactc ctaccagaac agagcaaggc aatctcttta aattaaccgc tcttgcaaaa
     4921 ctatctaacg aggatagtaa actcatccaa tatggcttac aaggtcgcgt cactagtgta
     4981 actacaaaga aaacatattt tgattatttc aaagataaaa ttttaacaca ttctgattaa
     5041 ttttcagata acactctata actatttatt atcttatcaa aaaggagaat cataacatgg
     5101 ataagaaaca aaacctaact tcatttcaag aactaacaac taccgaactc aaccaaatta
     5161 taggtggagg atggtgggaa gatttcttat atagatttaa tataattgaa caaaaaaata
     5221 caaaaggatt tcatcagcca atacaactat aaaaaaacaa gaccaagaga acgcactctc
     5281 ggtcttattt tttcttcatt ctatatgtat tacagtaagt acctgacgaa aaacgtgatc
     5341 ttggcaggtg gtattgagac tggtattggg atgactttcc acaattttca taacggtata
     5401 gagaccaact cctctctcct cccctttaga acttgctcca aaggagaaga tttcagaaat
     5461 atcgattccc tcttctttga tggaattttc aatgataaag gtctcctgtg ctccattttt
     5521 taaaaaggca attgaaacat gaggttgaca ggcttctaca ctagcttcaa tagcgttgtc
     5581 acaaaggata gacacaacgg ttagaaaatc aagtagactc atcccctcta cctgaatctc
     5641 ctctggaact tcgacattaa agacaatgtt cttatctcta gcttttagaa atttccctgc
     5701 taggagactt ttgagggctt tatcacgaat attcaccaat cgaccaaggt catatttatt
     5761 gtcctgcaat ttttcactag aatcctttaa gaccgagtcg tagatctctt ttatctgctc
     5821 catatcctcc tcttcaatgc ccagacgtaa gctagtcaag aggttggtgt agtcatggcg
     5881 aaaactccgt acttccttat aaagctcctc tatatgccga ctataccgct ccatctctct
     5941 gtagcgcaag tcctgctctt ggttcagtct ctcatggagt ttgtccttca aataggtatc
     6001 caatttcttg ataatcccca taaaaaagag caggtaaaag actaggatga gatggcgaac
     6061 agtcgttgat tgaatcccct gttcatattc aaagtaagac agattttgta tcaccaaata
     6121 gtaagccccc attatccagt taatctgagt cagggacttt tgaaaatctt tatcgagaat
     6181 cccctttctc aagctagtga aatcatagtc caaccatttc aaaaaagcta gagaaatgaa
     6241 gaaattgaaa attattatac ataaccaaat aaatgagtag tcatcatata cttgcccttg
     6301 tcccaaaaat ggaagcacaa aataggaaac acctctataa aagagattca ctaatatcat
     6361 tggaaagaga ccataaaaga gaaggagttt tttaggaagt tctcttaaca ataagaaaga
     6421 taagcctatg ccgtacaagg gttccataaa ataagataga taagtatttc ctaccatata
     6481 gccaagcatt ccaaaaacaa cggccaacag tatcttaaaa agaaaggcct taaaaatcct
     6541 ctcaaaagtg agactaattc catctacctt aaagaatatg acaattttta atccgtgagt
     6601 aacaagtgta tacaacaata tccaagcaac attcataaac tctcctagct cagtgtaagt
     6661 tattaatagc ctcagacact tccctgacct tataacgcgc gattagacaa cttccaccat
     6721 tgggaaagaa aagcagtttt tctttcttat ctaaatgcac cacattggca ggattgatga
     6781 gaaaagagcg atggcactgc aagagacgag gctcctgctt gagaacctcc tctagactcg
     6841 ctgtaaattc cagcctgtct gtcttggtat agagaataac acgatggggt cttgtggacg
     6901 tttcgagata gtaaacctct ttaaaaggat actggaattg agcaaatttt gatttaaagt
     6961 aaaagcaatc ttccgctaga cttttactgt cttgaccatt ggcatagagg agggctgtct
     7021 cgatacgaga ttcaaactcc tctgccgaaa gggccttatc aatatagtcc agagcagaca
     7081 cttggtagcg aaaggacagg ggcataaact ccgagtgagt cgtcacaaag acaatcaggg
     7141 catacggatc ccgatcccga atctttctag ccacttccag acccttcatc tcctcatttc
     7201 gaatctcaat atccaaaaag aatagctgat gggccccctt ctcatgcacc tctgccagca
     7261 gttggtctgg cttgccaaat acctcaaaag agctgggagt gatatgatgt tccttcaaga
     7321 gtttctcaat cgtcgtttca atcctagtct gttgggaaaa atcatcctct aaaacaaata
     7381 ttctcatctt cttactctcc ctctttcaac catttttgac gaatttctct atagcgacgt
     7441 ctggcatact tgacagcgta ttgaccttct tctccgagaa aaaggattct ttcttgaaaa
     7501 tcaatcgatt ttaatttatc aagattaacc aaacaatttc gatgacatct catcaaggtt
     7561 tccccatatt ggttctcaag attacttaaa ttttgaagca tattaaaact agcttcttct
     7621 gtaacaatct gtacggtatg ggctttagtt ggatgtgttt ggatatagta gatatcgtcg
     7681 acgtttactt tatagactgt tttctgcgtc tgaataatca tatgtttcat tggattctcc
     7741 tcctaagaaa agaataacag aaaagttcta gcccaagccc acccaatcct aaacagtctt
     7801 tgaaagctcc taaaaggatt atttttctac aacatcatga tgagcttaca actattttat
     7861 cataaaattc actcagcgca attcaagaca tttcaatgac aattaaagat ttgaataaaa
     7921 aaaaagaatt aaaaatgata aactaacagt atcaaaacac agttgccaga agtaagactg
     7981 gcacccagat taaaggaggc attcccatga cagatacaga ccccatcaaa agagctcata
     8041 ctttgattac tgacttaaac aaagcctacc aagcatgcaa acaggcaagc gctgacgacg
     8101 tccgctttca ggagcaatta aactctattc ttggtttttt agccaaggct gaaacagtgg
     8161 ataatcgatt cttgattgaa ctagaaaaat tttaccagac ttccagtctt ctcatgggac
     8221 tcagcgctct tgatccagat gctccaactc gcgccgcttg gcgggcctat gaccgcttcc
     8281 actttgacca agtcaagacc aagttaatac tcaatgaaaa tcaaagagca aactaggaag
     8341 ctagccgcaa gctgtacttg agtacggtaa ggcgacgctg acgtggtttg aagagatttt
     8401 cgaagagtat aagtctctac ggaccaacca ttatcctgta gtaacaaaaa agaagctgag
     8461 acagactgaa ctcaacttct tttttagtgt cgtatcagca atgttagtcc tgcatctgac
     8521 gttttacctg ataaggcaaa tacaagacta gtaaaaccaa accagctcct agtaaggcta
     8581 gaagggccag tttttcttgg aaggtaatca acccaacaac taattccaca agtaaaacaa
     8641 aactggtcaa tcctcgaact acctcctgta agcctttttc cttttgcccc ttgtccagcg
     8701 gaaagagttg ggtcaaatac tggtagtcaa aggcatgata gagggccagc aactggaaga
     8761 gcaagaggta gttaaagaga actaccactg ctgtcgcaat ccaagcttgc tcgataaaaa
     8821 cctgcgccag tacggaaagt aatagaagac gaagactgag agcaaagagg tcgccatttc
     8881 gcagataaga acgcagatag agattttgcc aaatcttccc aggcaccttc tgaacagcct
     8941 ttaagataaa gtccagatag gcacgacgct taacgctgtt tgaaattccc ttgacctgcg
     9001 taaagagggc aaagaaacga agcaagactt gcttacgctt gctttcttga gaaataacat
     9061 agtcccagtc cagtccagtt tcagtaaaaa atttgctggc cttttgacga aagtggaaat
     9121 attttcctac ccccaataaa agcacatata gcagaaaaac tggcaagcca taacccattg
     9181 ctaaaaataa aggcgcaaat aacagcaaga aaagggtctg tacaaagagc caaaagacta
     9241 gggaaatgcc agtttgacgc ttgagatgga gcttgattgc ctcttctcca actaagagaa
     9301 agagcttgtc tggagcctcc atataggtgg caattcctcc ccaaagtaag agtaaaacag
     9361 acgtaattcc tacaaacaaa aggataggcc aatgattttc aggaaaatgt tgtaagagtt
     9421 gactgtactg gtaggctaga aagcccaaca ggacaagcag gaacaagaca aagtggtcat
     9481 tgagcacata gcgcagataa caaagacact ccttacgaaa ggcctgcttt ctctttaaaa
     9541 acaagtcttt catagatcct cctctttggt cagagccaag taaatatcat tcaaactagc
     9601 ctcaggcata tcaaaggctt cacgtagttg caggagattg cctttggcac gcacctctcc
     9661 cttgtgaaga atgacaaagg catcacacat cttttccgcc gaatccagca cgtgggtact
     9721 catgagaata gacttgccct tttgcttctc cacttccaaa agctgaatca aatcagaaat
     9781 agccagcgga tcaagaccaa ggaaaggctc atccacgatg aaaagacttg gatccaccac
     9841 aaaagcacag ataatcatga ccttctgctt catccctttt gaaaaatgaa cagggaacca
     9901 gtctaatttc tgttccaaac ggaacatttt taacaagggc tctactcgtt cgaaagccac
     9961 tttttgctca ataccgtaag ccatagcaac cgtttcgata tgctctctga gggtcaattc
    10021 ctcatacaga ctaggcgtct caggaatgta gccaatctgc ttgcggtagc tagtcgcatc
    10081 tccttgcaga gtcaggccat tgatattgat ggagccacta taaggtgcca acagaccgat
    10141 aatctcattg atcgtcgttg atttcccagc accattgaga ccaatcaaac cgaccaactg
    10201 cccactttca acagtaaagg acacatcttt caaaacagga acatgaacat agccacctgt
    10261 caggttttta atttctaaca tattttctcc aaatctggta taatgtagct atattatatc
    10321 aaaattcagt acagtagagg tagattttat gtcagattgc attttttgta aaatcatcgc
    10381 aggggaaatt cctgcttcaa aagtatatga agatgagcag gtccttgcct ttcttgatat
    10441 ctctcaagta acactaggac acaccttggt cgtgccaaaa gaacactatc gcaatctttt
    10501 ggagatgaat gctacgagcg ccagccaact ctttgcccaa gtaccaaaag tagctcaaaa
    10561 agtcatgaaa gtcactaagg ctgctggtat gaatatcatt tccaactgtg aagaagtcgc
    10621 tggtcaaaca gtttttcata ctcacgttca ccttgtgcct cgctacagtg ctgacgatga
    10681 cctcaagatt gattttatcg cccacgaacc agactttgac aaacttgctc aagtcgctga
    10741 aaccatcaaa aacgcttaag gagttgctat gaaattatcc aacctactgc tatttgcagg
    10801 agctgcagcc ggaagttatc tggttacaaa aaatcgccaa accatcacag atgaagtctt
    10861 gaataccact gaccgcgttc aagctatcaa agacgatgtg gatattatcc aaaatagcct
    10921 gcaaatcatc gaccagcaaa aagaacttat caaggaatac caagaagact tgacttacaa
    10981 gtttaaggtc ttggaaaagg atatccagac tagactagct gtgataaaag aaatgcaggg
    11041 aactgaagat aagtaaaaag agccccccgg ctctttttta aaacgtaaac aagttatagt
    11101 gaattgaatt tagaatagca catcatagtt tctaaagcat tgttagagat tactttaaaa
    11161 tcctcttatc aatttgttca tattctattt caatctacta tatatatccc atcaactatg
    11221 catcataatt taggtaactc atactcaata aaaatcaaaa agcaaactag aaagctaggc
    11281 acagactgct caaaacaccg ttttaaggtt gtggatagaa ctgacgaagt cagctcaaaa
    11341 cactgttttg aggttgtgga tagaactgac gaagtcagta accataccca tgacaagccg
    11401 acgctgacgt ggtttgaaga aatttttgaa gagtatcatt caccatttca caactaaatc
    11461 gaagactttc tgccgtaatg aaaacacctg aaacagcttg gtttcagctg tccggaaact
    11521 ttaagactta ggttcaaagt ttaggtatgg aacttcgaag gaggtcgcta ccgtccgtca
    11581 ttacttagga aagtcttaaa aaatctataa agtaaaaaga gcccgacagc tctttttgtt
    11641 tatactcaat gaaaatcaaa aagcaaacta ggaaactagc cacgggctgc tcaaaacact
    11701 gttttgaggt tgtagatacg actcacatat atacggcaag ccgacgctga cgtggtttga
    11761 agagattttc gacgctgacg tggtttgaag agattttcaa agagtattat tctccatcaa
    11821 aggcatcttt aatatggtca aagaagcctt ttttctttgg atttactttc aagtcaccag
    11881 cagccgcgaa ttctttcaag gctacttttt ggcggtcgtt caagcctgtc ggtgttacga
    11941 cattaacagt aacgtattgg tcaccaactg caccgccacg aaggctcggt gcccccttac
    12001 tacgtaggcg gaatttctta ccagtctgag ttccctctgg aataaccaat tcaacatcac
    12061 cgtgaacagt tggaatatct actgtatcac caagagccgc ttggacaaag ttgaggttga
    12121 gattgtagaa gatagtcgtt ccttcacgtt caaacttgtc gctagcttct acagaaacta
    12181 ctacatacaa gtccccataa ggtccaccgt taaagcctgc ttcgccttga ccagcgaggc
    12241 gaatttgttg acctgtttcc acaccagcag ggattttcac atgtacgcta tgagcttgtt
    12301 tctcatgacc tgttccatga caggttgtac atggatattt gatttctttt cctcgaccgt
    12361 gacagacatc acaggttact tggcgacgca tcataccaag aggagtctgc gtatcgacgt
    12421 taatgacacc agcgccatga cagcgtccac aagtgactgg acttgtccct ggcttagcac
    12481 cagagccatt acatgtacga cagccagctt cacgatgata cttaacttcc ttctcagttc
    12541 cgaagatagc ttcttcaaag gtcaaattga cacgatactg gagatcatct ccttggcgag
    12601 gagcgtttgg attgcgcgaa gaaccgcctc cgccgaagaa acttgaaaaa atatcctcaa
    12661 aaccaccgaa gccacctgcc ccattgaaac caccgaaacc accagctcca ccaaaaccac
    12721 cattggcgcc tgcagcacca tactggtcat aggcagcacg tttttggtcg tcactcaaag
    12781 tctcataggc ttcttgaact tccttgtact tgtcctcagc accaggctcc ttgttgatat
    12841 ctgggtgata ttttttggaa agcttacgat aagccttttt gatctcgtct gccgaagcgt
    12901 tttttgacac ccccagacga tcataaaatt cagtattgtt catacaagat accaagaccg
    12961 taaaattcga ctgaaaaata ggaaatctga cgctggagcg acgctcctag acagatttct
    13021 cttttttccg agaatttagg tcgggttcaa ttcctttctt ttatattgat gagataatac
    13081 tccagccact cggccctatc cctttttacc gaaaaacaga ggctgagttg caacctctgt
    13141 ggttagatta ttcatttgaa ataaaaatgg ttgtattatc tctagtagta atcttcaaag
    13201 accttttagc taaatcaaac tcaaatttat tatagtgtgg gtcttgtgtt ttatataggt
    13261 gtaactcctg acatatatga tccgaaaagt aaatcataac atctttaaac acagaagtgt
    13321 ggtcatattt actcttacca acagctactt ctgtaaattc tttataaatg acatttccat
    13381 ttctttccac agtcaccagc atttctgttg gattataatc caaattaaat aattcttcaa
    13441 aaaactttat cttttcatga taatctgcaa tgtcagaaag attattttta ttaacactca
    13501 taacactagc cccttatttt tcagtaaact ctccgtctac gacgtcatcg cctgcgtttc
    13561 ctgttgcttg tgcgccttct gctccttctt gagcttgttg cgctgctgca gcttgttcgt
    13621 agagtttaac agcaagtcct tgagcttttt cgttcaatgc ttcaagtttt gctttcatgt
    13681 cgtccaagtt gttgtcttct tgagctttct taaggtcatc aagggcagct tgggcagcgt
    13741 cacgttctgc gtcgaagcct ttaccttcag tttccttgat tgtcttttca gtcgcaaaga
    13801 ttgcttggtc tacttcatta cgaaggtcta cttcttcttt acgtttctta tcggcttcag
    13861 cgtttgcttc tgcatctttc atcatgcggt cgatttcttc gtcagtcaaa cctgagttcg
    13921 attggatgac aatagtttgt tctttttgag ttccaaggtc tttggcctta acagacacga
    13981 taccgttctt gtcgatgtca aatgttactt cgatttgagg aattccacga ggtgcagctg
    14041 ggatatcagt caattggaag cgtccaagag tcttgttatc tgctgccatt gggcgttcac
    14101 cttgaagaac gtggatatca acggctggtt ggttgtctgc tgctgttgag aagacttgtg
    14161 atttagatgt tgggattgta gtgttgcgat cgataagttt tgtaaatact ccacccattg
    14221 tttcgatacc aagtgacaat ggcgttacat caagaaggac aacgtccttg acatcaccag
    14281 taatcacacc accttggata gccgcaccca tagcaactac ttcatcaggg tttactgatt
    14341 tgtttggttc tttaccagtt tcagctttaa cagcttcaac aacggcaggg atacgagttg
    14401 aaccaccaac aaggataact tcgtcgattt ctgacaagct caaacctgca tctgaaaggg
    14461 cttgacgaac tggaactttt gtacgttcaa caaggtcacg agtcaaatcg tcaaatttcg
    14521 cacgagtcaa agtcatttcc aagtgaagag gtccagcctc acctgcagtg ataaatggca
    14581 agctgatttg tgttgaagtt acaccagaaa ggtctttctt cgctttttca gccgcatctt
    14641 tcaaacgttg cattgccatc ttgtcagtag acaagtcgat accgttttct ttcttgaatt
    14701 ctgctaccaa gtggtcaatg attttttggt caaagtcgtc accaccaagt ttgttgtccc
    14761 ctgcagttga caatacgtcg aagacaccgt cacccaattc aaggatagag acgtcgaatg
    14821 taccaccacc aaggtcaaat accaagattt tttcttcttt gtcagtcttg tccaaaccat
    14881 aagcaagagc tgctgcagtt ggttcgttaa caatacgttc tacttcaaga ccagcaattt
    14941 taccagcgtc ttttgttgct tgacgttgag cgtcgttgaa gtaagccgga actgtgataa
    15001 cagctttggt tactttctca ccaaggtagt cttcagcgta gcctttcaag tattgaagga
    15061 tcatagctga gatttcttgt ggagtgtatt cttttccatt tgcagaaact ttttcagaag
    15121 ttcccatctt agatttgata gagataactg tatctgggtt tgtaactgct tgacgttttg
    15181 cagcatcacc aacgatgatt tctccgtttt tgaatgagac tacagatgga gttgtgcggt
    15241 ttccttctgg gtttgcgatg attttgcttt cagttccttc aagaactgca actgctgagt
    15301 ttgttgtacc taagtcaata ccgataattt tagacatgtg tttttctcct tgttagttaa
    15361 ttttctattt ttctttttga tactcttctt aagtggcgac gccgtcagag cttgacttcg
    15421 tcaatctctt tgactaaact ttgagcctaa ggtctcaaaa tttgcgcaaa tagcaccacg
    15481 gcgaagagta tcgtctttgg ttcgcttcgc tcactattac aaagccaaac atattttatc
    15541 tttcttcata gtttattgtc gtttcggaca agttttctat tatgtaaatt gcgacacgag
    15601 gagtcgaaat cgattttatt tcgacgacga gttagtaagg aagctaggca aacgtcatag
    15661 cgattgccgt tttctgacga gttgctttag ctaccgtcag aattgccgaa tcgaacacgc
    15721 cctaagctct gagtaaaaaa gatagatttt cttgtgttca tcgaacactt cgtcaatctc
    15781 ctatttttac tgcgagcttt ttacgagctt tgtatcttag ttatacacca ctaccattgc
    15841 tgggcgtagg atgcggtcat ggagtttgta gcctttttga aagacttggg cgatggtatc
    15901 tactgggtgt tcatcgtctg ctgggagagt ttggatggcc atatggtagt tatggtcaaa
    15961 ttcgccatct gctgcgattt cttcaattcc ttcttctttc aaagcgtgaa tcaagctttc
    16021 ttgcaccatc cccaagccct tcttcacatc atctgtcaaa ccttcaactg caagtgcacg
    16081 ctcaaggttg tcaagagatg gtaagattgc ttttgccaag tcctggctac gataacgttg
    16141 caagttttga cgttcttcat tggcacggcg ttggatattt tgcatttctg catgagcgcg
    16201 aagatatttg ttttcgaact catctgcacg ttcatttgcc aagtccaact cagacttttc
    16261 aggagttgtt tcttcagctg tttccacaac ttcctcttct tgaacttctt ctacttcttc
    16321 attttttata tcttgggcca tgttcgcctc ctttaatgat ttcaatctta atgtacttcg
    16381 taatgattac tgctgaggta gcggtaaaaa tctgtcaact tcatggtcaa aacacggttg
    16441 accacattga cttgattgat tagctgttgg taatccagat taactggacc gataatggct
    16501 agaattccaa ctccccgata aggaatgagg aacttactac taatcaccgc taggtcagct
    16561 aaacaggact cttgaccgtc tgcaacacga acattttgca tctgatcctc acgcaacccc
    16621 tcacgaatct ccaaggccac cttttgcggt tggtcaaaga actgataggc ttctagattg
    16681 gcaaaattca agagattgac cttgcccgcc atcacaatgt tttcgttgaa catttcctta
    16741 aagatgtgtt caaagagatc gatgacatta tccgttgttg taaagtaacg ctggataatc
    16801 tgcggaatct ccgtccgaat cttgtagtga atatctaaaa cggtgtgacc gaggaaacgt
    16861 tcctgaatga tgctcttcag tttcagcaaa tcctcctgca agaagttcct tggaatcaga
    16921 aactgactag taaccgttcg cgactcgtct agggtaaata ccgccaaggc tgtatgttgc
    16981 cccaaaacaa cgatatcaaa ggctgtcaaa cgttgcctgc tcggctcaac atccagtgcc
    17041 actaccgtac agccactcag gcctgttagc aagttagcag cctcttgcag aatatcctcc
    17101 aatttgaaga attcctgatc aaaggctttg acaatctcat atacctcatt ttcagccagc
    17161 cggtgaaaat ccagtgagtg tttcacatag tactgaaaac cagcaacact tggcatccga
    17221 ccgcttgaag tatgagcctt ctcaagcaac ccttgttttt ctagttccgc catgtcatta
    17281 cgaatggttg cactgctaga gttaatagac tcttgcaagg cttttgatcc gacaggttcg
    17341 tgcgttttgg taaagatgtc aataatcaga tttaaaatat cctgctgacg ctctgtaacc
    17401 atctcatcac tcctctctgt ctgatcaatt agcactcaat gcatccaagt gctaacttat
    17461 gattctatta tacaccctta gaagagaatg tcaagacaaa aactcaaaaa attagcactc
    17521 ttttatcaag agtgctaaaa atcaatctga agacctagaa ccttgctatt catctactcg
    17581 gtatttcctt tttagtctga aataaccatg aattagataa gaaatcagaa aagcatataa
    17641 aacaaaaata agaaagaaag acatagaaag atcctctcta aacaaactcg tacaaacaac
    17701 aagaccacct aagaaagtag ttgtacagga acgaagtcta gatcgcataa cttcccattt
    17761 gagcccctta ctcatataga cttgatcctc tggaagtaaa tcagaagacg atttacgaag
    17821 aatcgaacaa gaacctgctc ctatcaattc ccaacctcta tctctaaaat cttgcagttc
    17881 atgcttatat tttttaagaa atctagaatc atagatacgg tagatgacat cgtctggttg
    17941 acattggtca aaatagaaca aaccaaaacg actcgttcta tacctccaac ctttcaaatg
    18001 catctcatgt aaatattctt cttccttgtc caaatcaaca atagtgaaaa tccgaaattc
    18061 tactctgcta ttcattgtct taccccaaaa ttagaaaaca tgcctggcgt tatttattag
    18121 ataattcttt ccgcttttga ctcaatctcc aaaaaatata agaaatctga atcgcaaaaa
    18181 ctatcaataa aacccaatct attatgaaag tcaaaaacac ttcccaactg aaagaactac
    18241 ctccagtgac aaactttgag aaaaacggta gtagagctaa aaagagaagc aaaataggaa
    18301 gcatccgcat tgttaaaatc cttttggcat aaaaaaatct ttatttaaac gaaaatatta
    18361 tggcaaaatt tacgccagtt tttgaacggc tgatgtagat attttatact ttcaaaatgt
    18421 ttaaatgtga ttatttattt ttgaaaaata gctcaccagc ccgactgaaa gtgcttataa
    18481 atgataataa gtcgcctgcc gaaaacagcg aaaaatagcg gtgttatgcg gagataatct
    18541 gacgcgatgc gaaagtatat tgcatactta ttttcaacaa tttagcagag tatttttata
    18601 agtgtgatat aatagaagta taatttgttc tgatagttta ttttatggag aagtagattt
    18661 ttagaatgcg gagggttcaa tatggttgag tttataaagt ctaagaaaga aatgagtgag
    18721 gaggatatta aagcaaattt catcactcct gctattgtat ccaaaggatg gaaaaatggt
    18781 gagcatatcg cttacgaaga atacttcact gatggtcgaa ttgaggttag aggagataag
    18841 gctcgtcgta aagaaggaaa aaaatcagac tattcactgt attaccaatt tggaactcga
    18901 attgcaattg ttgaggcaaa ggataataaa cacagcgttc gagcaggatt acaacaagct
    18961 attgaatatg gagagatttt agatgttcca tttgtttatt cttcgaatgg tgatggcttt
    19021 attgaacacg accgtatcac gagagaagaa cgtgagctgg agttagacga attccctact
    19081 cgtgaagaat tattttctcg tatgacgaag gaaaaaggat tgacgtacga aattacagaa
    19141 gctatctcaa ctccatacta tacagacgcc ttctcaatga aaacgccacg ctattatcag
    19201 caaatagcta tcaaccgtac tattgaaaca gttgccagag gacaaaaacg agtaatgttt
    19261 gtgatggcaa caggaacggg gaaaacgttc atggcttttc aaattattca tcgccttcga
    19321 aaagctggtt tggctaaacg agttttattc ttagcggata gaaacatctt agtagaccaa
    19381 acgatggctg aagactttag gccattcgaa aaggtaatga cgaaaattac accaaaactt
    19441 ttgactgctc ctgaaaaatt aaattctttt gaaatttatc tagggcttta tcagcaacta
    19501 actggtgaag atggaactga aacacattat caaaaatttg acaaagactt ctttgattta
    19561 atcgtaattg atgaagcgca ccgtggttca gctaaggaaa acagtaactg gcgtaagata
    19621 attgattatt tcagttctgc gacacagatt gggatgaccg ctactcctaa agaaaccaag
    19681 aatgcttcca atacggaata ctttggtgag ccaatctata cttatagttt aaaacaggga
    19741 atcgaggatg gttttttggc tccatatcgt gttatgagag ttaatttaga tgtagatgtg
    19801 gatggttatc gtccagaaac tggaaaagtt gatgctaacg gacaattaat agaagatagg
    19861 tactacggca ggaaagattt tgataaaacc attgtcattg atgatagaac gcaaagagtt
    19921 gccaagtttg tttctgatta tatgaagcaa aacaatgcac gatttgataa aacaattgtt
    19981 ttttgtgttg atattgacca tgccgagcga atgcgtgctg cacttgtaaa agagaatcta
    20041 gacttagtcc aagaagacta tcgttatgtc atgcaagtaa ctggtgacaa cgctgaagga
    20101 aaagctcaac tggataactt tatggatgtc aattctaatt ttcccgctat tgtaacaacg
    20161 tctaaattat taacgacagg agttaatgct aaaacatgtc gtttgattgt tttagactct
    20221 aatatccaat ccatgactga atttaaacaa attattggtc gtggcacacg tctttatcct
    20281 caaaagggga aagaattttt tacgattatt gattttcgaa atgttaccaa tttgtttgct
    20341 gaccctgatt ttgatggtga tccagtgaag gtgctggaaa caggtgcgaa aacagtcagt
    20401 ggttctacgc ccggtttcgt aagtgaggaa ggtgacccag tagaaaaata tatcgttaca
    20461 gacaagcagg ttaccattct taattctact gttcaagtat tggatgaaaa cgggaaactg
    20521 attaccgaaa gcctgaccga ctacactcgg aagaatatct taggtagcta cgccactttg
    20581 aacgatttta tcacagtttg gcatacggcg gataaaaaga agcttatctt agacgaactt
    20641 tataaaaaag gagtttatct agatgctatt cgagagtcgg agggaatatc agaacaagaa
    20701 atcgatgatt ttgatttact cctaaaactt gcctatggtc aaaaagaatt aaccaaaacg
    20761 gaacgtatca ataagctcaa acaaagcgga tatttatata aatatagtga ggaagcgcgt
    20821 gctgttttgg aaattttact gaacaaatac atggataaag gtattggaga actcgaaagc
    20881 attgaaacat taaaacttcc agaatttcag atatatggtg gaaccttcaa aatcatcaat
    20941 acttattttg gagataaaaa acgatattta caagcaatta aagaattgga gcaagagcta
    21001 tttacagtag cttaatgaaa ggaaagtatg tcaattacat catttgtaaa aagaattcaa
    21061 gatatcactc gaaacgatgc tggtgttaat ggtgatgctc aacgtattga gcaaatgtct
    21121 tggttattat tcttaaaaat ttatgatagc cgtgaaatgg tttgggaatt agaagaagac
    21181 gagtatgagt caattatccc agaggaatta aaatggcgaa attgggctca tgctcaaaat
    21241 ggggaacggg tattgacagg cgatgaatta cttgattttg tcaataacaa gttattcaaa
    21301 gagttgaaag agcttgaaat aacttcaaat atgcctattc gaaaaacgat tgttaaatca
    21361 gcttttgaag atgcgaacaa ctatatgaaa aatggcgtct tgttacgcca agtcatcaat
    21421 gttattgatg aagttgattt caatagccct gaagatcgtc attcgtttaa tgatatttac
    21481 gaaaaaattc ttaaagatat tcaaaatgct gggaactcag gagaatttta tacgccacgt
    21541 gcagcgactg attttattgc cgaagttctt gacccaaaac ttggagaatc aatggcagac
    21601 cttgcttgcg gaacaggagg cttcttgact tcgactctga accgtttaag tagtcaacgt
    21661 aaaactagtg aagataccaa aaaatataat acagctgttt ttggtattga aaagaaagca
    21721 tttcctcatc ttttagcagt tacaaatctg tttcttcacg aaattgatga ccctaaaatt
    21781 gttcatggaa atactttgga gaaaaatgtt cgtgaatata cggatgatga aaaatttgac
    21841 attattatga tgaatccacc ttttggaggg tcagaattag aaacaataaa aaataacttt
    21901 ccagcagaat tacggagttc tgaaacagct gatttattta tggctgtcat tatgtatcgt
    21961 ttgaaagaaa atggtcgtgt tggagttatt ttacctgatg gttttctatt tggtgaaggt
    22021 gtaaaaactc gcttgaaaca aaaactggta gatgagttca acttacatac gattattagg
    22081 ttgcctcata gtgtctttgc accgtataca ggaatccata cgaacattct tttctttgat
    22141 aaaacaaaga aaacagaaga aacttggttt tatcgtttag atatgccaga tggttataaa
    22201 aatttctcga aaactaagcc gatgaagtca gaacacttca atcctgttcg tgactggtgg
    22261 gaaaatcgtg aagagatttt ggaaggtaag ttctacaaat ctaaatcatt tacacctagt
    22321 gaattggctg agttgaatta taatttagac cagtgtggct ttccaaaaga ggaagaggaa
    22381 atcttaaatc cctttgagtt gattcagaat tatcaagcgg aaagagcaac tttaaatcat
    22441 aagattgata atgtattagc tgatattttg cagttgttgg aggacaaata atgacaccag
    22501 aacaacttaa agcaagtatt ctccaaagag cgatggaagg gaaattagtg ccgcaaaatc
    22561 ccaatgacga acctgcaagt gaattattaa agagaattaa agctgaaaaa gaaaaactta
    22621 tcagtgaagg aaaaatcaaa cgagataaaa aggaaactga gatatttcgt ggtgatgatg
//
LOCUS       DAVBGY010000029        22656 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVBGY010000029 DAVBGY010000000
VERSION     DAVBGY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.029"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            87..3437
                     /locus_tag="VQ620_001166"
     CDS             87..3437
                     /locus_tag="VQ620_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET2763797.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            3681..3782
                     /locus_tag="VQ620_001167"
                     /pseudo
     CDS             3681..3782
                     /locus_tag="VQ620_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4023..4493)
                     /locus_tag="VQ620_001168"
     CDS             complement(4023..4493)
                     /locus_tag="VQ620_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2763798.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4510..6783)
                     /locus_tag="VQ620_001169"
     CDS             complement(4510..6783)
                     /locus_tag="VQ620_001169"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HET2763799.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFAYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEVELEVKLPHKIELEEPQLNLVQPQGQAELVAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQIFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCKTFSLGQEEQ
                     AIQNQYPDKDFERYSKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            7129..10230
                     /locus_tag="VQ620_001170"
     CDS             7129..10230
                     /locus_tag="VQ620_001170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET2763800.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRVAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAIIVPYFDRVESLELGCDYYIGVYPETLASEFHRPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALENLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGFVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            10313..11320
                     /gene="pfkA"
                     /locus_tag="VQ620_001171"
     CDS             10313..11320
                     /gene="pfkA"
                     /locus_tag="VQ620_001171"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET2763801.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            11379..12884
                     /gene="pyk"
                     /locus_tag="VQ620_001172"
     CDS             11379..12884
                     /gene="pyk"
                     /locus_tag="VQ620_001172"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET2763802.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(13618..13843)
                     /locus_tag="VQ620_001173"
                     /pseudo
     CDS             complement(13618..13843)
                     /locus_tag="VQ620_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            14691..15563
                     /locus_tag="VQ620_001174"
     CDS             14691..15563
                     /locus_tag="VQ620_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HET2763803.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(15709..16053)
                     /locus_tag="VQ620_001175"
                     /pseudo
     CDS             complement(15709..16053)
                     /locus_tag="VQ620_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            16196..16508
                     /locus_tag="VQ620_001176"
                     /pseudo
     CDS             16196..16508
                     /locus_tag="VQ620_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16512..17228
                     /locus_tag="VQ620_001177"
                     /pseudo
     CDS             16512..17228
                     /locus_tag="VQ620_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(17358..18173)
                     /locus_tag="VQ620_001178"
     CDS             complement(17358..18173)
                     /locus_tag="VQ620_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HET2763804.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(18170..19075)
                     /locus_tag="VQ620_001179"
     CDS             complement(18170..19075)
                     /locus_tag="VQ620_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET2763805.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            complement(19405..19488)
                     /locus_tag="VQ620_001180"
                     /pseudo
     CDS             complement(19405..19488)
                     /locus_tag="VQ620_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            19532..21661
                     /locus_tag="VQ620_001181"
     CDS             19532..21661
                     /locus_tag="VQ620_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HET2763806.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDFFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            21648..22097
                     /locus_tag="VQ620_001182"
     CDS             21648..22097
                     /locus_tag="VQ620_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HET2763807.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTRRYRCGLCRGKLLLINQPED"
     gene            22156..22428
                     /locus_tag="VQ620_001183"
     CDS             22156..22428
                     /locus_tag="VQ620_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET2763808.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
BASE COUNT         6991 a         3968 c         4880 g         6817 t
ORIGIN      
        1 gcttgtgaga tagctataaa agtgtggaga aatagcttga aatttagtat aatatagcta
       61 aactatttgt ttaaagtgag aaaaaaatgg gaaattttag ctttctttta aaaaatgacg
      121 aatatgaatc tttttcaaaa ccttgcattg aagctgaaaa tatgattgct acatcaactg
      181 tggctactgc ctttatggcg cgtcgtgctt tagagcaggc tgtccattgg atatatagtc
      241 acgattcata tttagaagct ccctatcgtg ctactctatc ttctttagta tgggatgatg
      301 attttaggga tatcgtagat tctgaactcc acaagcagat agttctgttg attcggtggg
      361 gaaaccatgc tgctcatggt ggtgaaatta aggaacgaga agcgatttta gctttacatc
      421 atttgtatca gtttgttaat tttatcgatt attgttacag caatgaattt gtggagcgtt
      481 attttgatga gaagtgctta ccactttcag caaacatcaa ataccgagaa actccacaat
      541 ctatgataaa gttacaagac agtttaccag aactgcctga ttttcatgaa cagatggctg
      601 ctcagtccgt agaagttcaa gagacttata ctgaaaaacg tgagactgca gcgcaacggc
      661 aagatgtgcc tttccatatt gatcaattat ctgaggcaga gacaagaaag ctctttattg
      721 atatcgatct ccgtttagca ggatggatat ttgaagaaaa ctgtcgtgtt gagatagccg
      781 ttgatggtct caagcacggt tcaggaattg gttactgtga ctatgtactt tatggtaaaa
      841 atgggaaaat tttagcgatt gtagaggcta aaaaagcctc tgtcaatcca gaagtagggg
      901 aagtacaggt caaagaatat gctgaagctc ttgagaaaca tatcggctat cagccaattt
      961 gctttattac aaatggcttg aagcactata tacttgatgg tccgaaccgc cgccagattg
     1021 caggctttta ctctcaagaa gaattgcaat tagtgatgga tagacgtcat cttcaaaaac
     1081 cacttgagga tatttctagt aaaattaggg acgatatttc cgggcgtcac taccaaaaac
     1141 acgcgattgc aagcgtttgt gaagctttct ctaatcatcg tagacaggca cttttggtta
     1201 tggcaactgg ggctgggaaa actcgtacag cagtttctct agttgatatc ttatcacgtc
     1261 ataactgggt aaaaaacgtt ctcttcttag ccgatagaac ttccttggtt aagcaagcat
     1321 atgattcgtt tagaaaatta ctcccagatc tttccgtttg taacttctta gaagataaag
     1381 aaggagctca atcaagtcgc atggtctttt caacttatcc gaccatgatt ggagcgatta
     1441 gtggtcaaga agaagtaaat caacgccctt tcactgttgg gcattttgac cttatcataa
     1501 ttgacgaatc tcaccgttct atttatcaga aatacaagtc catttttgat tattttgatg
     1561 caagaattgt aggcttaaca gctactccgc gtcaagattt agataaaaac acctatggat
     1621 tctttaattt ggagaatggg gttccaacat atgcatatga tttggaagag gctgttaaag
     1681 acggatattt agtagcctat cattctatcg aaaccaaact gaaactacct acggatggtc
     1741 tacattatga tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa tttgaagacg
     1801 atagctgtga aaaagatatt gatgggagtg tatttaattc ctttgttttc aataaaagta
     1861 cagtagaaat tgttttaaat gaactcatga caagaggaat tcagacagcc tcgggtgatg
     1921 aaattggtaa aactattatt tttgctaaaa atcatgatca tgcggaatat atcagaggta
     1981 tttttaacaa ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt gattatagta
     2041 ttaagcatta tcagaccttg attgatgatt ttaaaattaa ggagaagtat cctcaaattg
     2101 cgatttctgt cgatatgtta gatacaggta ttgatgtacc agaggttgtt aatttagtct
     2161 tcttcaagaa agtacgctct aaaactaagt tttggcagat gattggtcga ggaacccgtc
     2221 tatgtaaaga tttatttgga cctgagcagg ataaggaaaa cttcttggta tttgattatg
     2281 gggacaattt tgattatttt cgtgcagatc caagagatgg agagggtcgt cacattgttt
     2341 cgttgactca gcgtttattt aatatcaaag tggacttgat tcgagaactt cagggactcc
     2401 aataccaaga agatcagttt gcgagagcat accgtcagca gcttgtctcg gaacttcaag
     2461 gtcgtataga gagcttaaat gagttggact tcagggttcg tatggtttta gatacagttt
     2521 atagctatag gaaattggaa agttggcaga atctaactgc tgttacaagt gaaaccattc
     2581 aaaaaaatct ctctccgctt ttatttgatg aagataaaga agatgagatg gcgaggagat
     2641 ttgatttgtg gttgcttcat attcagttgg ggcaactgac agctaaatct tccactgttc
     2701 atatttccca agtgatgaag acggctagag ctctttctgc tattggcaat atcccgcagg
     2761 tttttgagca ggctgaaatt atcaggaaag tacaggagcc tgaattttgg aaagaagtta
     2821 acttgtctga tttggaaaaa attcgtcttg ctattcgaga tttattacag tttttggata
     2881 aaacagaccg taaaccctac tatgttaact ttgaagatcg tatactctcc actgttcacg
     2941 agaccacagc atttttgcag gtcaacgatc ttcggtctta caatgaaaaa gttgagcatt
     3001 atttgaaaac tcatctggat gaggagtcca tttctaagct ataccataat aaaaagttga
     3061 catctgatga tatgcttgca cttgaaaaat tgctttggga aaaattaggt agtaaagcag
     3121 actaccaaag tcattatgaa aataaggcaa ttccgagatt ggttcgtgag attattggct
     3181 tagatagaga gtctgccaat cgtatttttt ctaaattttt gtcggatgag aatcttaatg
     3241 ccaggcagat ttcatttgta aaattgattg tagactacat tgtagaaaat ggttttttag
     3301 agacgaaagt gttaacgcaa gagccgttta aatcttatgg atctgttcaa ctactcttcc
     3361 aacaccaact accagtactt cgtaatattg ttcaaatcat tgaacttatc aataatcgag
     3421 ctggagaagc ggcttaaatt ctaaagtgat tgccatgctg agactcattt aaaattaaaa
     3481 agagtagaaa tttatgctat atatgagaag ttttattagg aagaatgtca tcgttttcct
     3541 agaatacagt atcagttgtt aagtggctga taaatttcaa agtagatact tgtaccacga
     3601 tgtttgttga tagagttatt aacaaaagag ctactttgat tttaaagaaa tagaaaacaa
     3661 aaagccgagc aagaattcaa ttgcaggaga aaatgaaata atactcaatg aaaatcaaag
     3721 agcaaactag gaaactagct gcaggctgct caaaacactg ttttgaggtt gtagataaga
     3781 ctgacgaagt cagctcaaaa catggttttg aggttgtaga tgaaactgac gaagtcagta
     3841 accataccta cggcaaggcg acgttgacgc ggattgaaga gattttcgaa gagtataaat
     3901 cttcctagga taaagcaaaa cgcatagtat caagtgttga aaacccttga tactatgcgt
     3961 tttctgatgt taaagacttt ctaccagttt ttttaagcat aattgttagt tgtagtcatt
     4021 tattattctt caaagaaaaa tggtggggcg aattttttca gttcttcaaa gcacttttga
     4081 gcagtatctg catcttcaca gatgataaga cagacatcat taccacaaag ggtagcgata
     4141 gcgtcaggga agctcaaagt atcaatgata gaaccaaagg attgagccag tccaggaagg
     4201 gtttttagta ggacttggtg ttgaactggg cgcatccaga caagggcgtc ttccatgtag
     4261 agttcgagac gtttttccca ttttgagatg gaaccattgt taagaacata ataagcgcta
     4321 tcttcttcgc ggacttttga taggttcata tttttgatgt cgcgtgagag ggttgcctgg
     4381 gttacttgaa tgtcgttctt agcaagaagg gcttgcaact cagcctgtgt atgaatcttg
     4441 ttttttgtga taagagcgcg tataagttgg tggcggtgtt ctgatttatt cataataatg
     4501 taactccttt tagcaaggta aggtaagcat ggactgagcg aggtcgacag tcaagtggta
     4561 gtctgtattg tcacggatgg tgatttcaaa gtcagtagta tagaggacta aacggagagt
     4621 gtctccttct tttagcttgt aaatagttgg ctgcagttca aattgaacgt ccatccattc
     4681 atctgcagta atatcctcta ctaacagtaa atcatttcta ttttgtaaat taaggtaacc
     4741 ttttgtcacg actcgttgtg cctctggtct aaatggcaat tcacagagat tttccaacat
     4801 gtgatagcga ccgttgtcaa tggttctagc acttaaaata gctggataag gttgtaggta
     4861 tttcttttgc ccaaattcta gcagttgggc agataagagc cccttgtttg tactggattt
     4921 gatacgaaga ttgagctgag cgcgaccgtt taggtggaga tctttagtca caggaaggtt
     4981 aatagtaatc tgattagctt tcccttgata gagctctgta ttgaaggttt ggtatgtctt
     5041 actatagcgc tcaaaatcct tatctgggta ctggttttga atagcttgct cttcttgacc
     5101 aagtgagaag gttttacagt tttcttgccc accgaagtta tcaagtgata accaagtctg
     5161 tggagctgta ttgtcctgcc agataacagt aggaagttga aagtctgttt cctgtcctag
     5221 taatttcttg gttaataagg catttatgga ctcacggaag tcaattgatt gccaattgtt
     5281 catgtaaaca tgggcaccat tatggaaaaa gagatgcttg tgtatatgag taggaagagc
     5341 atggaatatc tggtaaacat gaagtggttt gacattccaa tcctgagaac catgagtaaa
     5401 gacaacctct gcctttactt tatgggcatt gagcagataa ttgcggtcat gccaaaactg
     5461 attgtagtcc ccagtttttc ggtctagctg agctttcact ttttctaagt cagcttggtg
     5521 agcttcattg ccacggatat agtcgccagc taagagatta cgagaatagg ttaactcagc
     5581 aagggagtca aagtcctcac ctggataacc acctgggcta gtcaccagac cgttttcacg
     5641 gtagtagttg taccatgatg aaattcctgc ctcggcaatg ataacttcta aaccatcgac
     5701 tcctgtagtc gcaagaccat tggacatggt acctagatag gaaagtcccg ttgtagcaac
     5761 ttttccgttt gaccaatcag ccttgacttg acgctggcgc gtgtgatcag taaaggcacg
     5821 gcaacggccg ttaagccaat cgatgacatt tttataagcc tcgatttgct ggtagtctcc
     5881 attagtcatg aaacctgtcg agtctttggt accaacacct gagacataga gattggcaaa
     5941 gcctctcgga aggaagtagt cgtttagtgt atagctagag ttgatgtgag ttagcttttc
     6001 ctcagcctct gctacaagct cagcttgacc ttggggttgg acgagattta gttgaggttc
     6061 ctctagctca atcttgtgag gaagcttaac ctcaagctca acctccatct tgtagagagc
     6121 cttgtcacta gccttgtcat tggttccctg atgataaggg ctggctgtca tgatggcagg
     6181 gatttttcca tcaaaacgag ggcgaataat gctaaccttt actaggtctg atagcccttt
     6241 ttggtcagta tcgacacgag actcaacgta aacgacttca cgaatgacat cctggttaga
     6301 aaaagtagcc aaactcttgc cgttaaagta gtggtagtca ttatcctccg gaataagacc
     6361 atcactaaca agttggtcga taagagtatt tccttttttg gtgcgagtat tgagtaactg
     6421 atagagattt tcaatcaagt caccatatat aatgggaaat ccagtttctt tacgaaaaac
     6481 gtcactatct tcgaagtcaa ccaaataagc aaagcctaaa agttgaaaag caacagtata
     6541 aaaaatatct gctgtcagtt catcttctga ttgaaaaaat gtcagcaggt ctgttttttt
     6601 atcagctgct aggatagaaa gtgggtagtt ggtgtcttga taagtgaaaa agaaacgacg
     6661 taaaaaggtt tcaagtgagt ctttgtgatt ggctgtattt tgtaaatcaa agccacattt
     6721 ttttagttca gataagacat tttcttttgg aaaattgata taactatatt gattaaaacg
     6781 catagaacct ccatatagaa tgacagttaa ggttattata tcaaaaaaaa agcagaaagg
     6841 gaattgttaa cttcaaaagg aaataatcca ataaaaatga ataaagtact aaattcaata
     6901 tagagaacag agtaacaata agaataatag atagggtata aaagttctag gaaatttata
     6961 gtatatgctt tctgttttta tatacaatat agtataaata taaaaacgat gacaaaaata
     7021 caaatgaata gaaaataaat tagtaagctg atgaaatttt tctcaagaga agccatttat
     7081 aggtgaaaat ggtataatat agtgagaagg atagaggaga agtgtaaatt gatcgcacaa
     7141 ctagatacaa aaacagtcta tagttttatg gaaagcgtca tttcgatcga aaagtatgtg
     7201 agagtagcta aagaatacgg ctacactcat ttggctatga tggatattga caatctttat
     7261 ggcgctttcg actttctaga gattacaaaa aaatacggca ttcatccttt gctagggctt
     7321 gaaatgacag tgtttgtaga tgatcaggga gtgaatttgc gctttttagc tctatctagt
     7381 gtgggctatc agcagttgat gaagctttcg acagccaaga tgcaggggga gaaaacttgg
     7441 tcagtcctgt cccagtacct ggaggatatc gcgatcattg tgccttattt tgatagagtt
     7501 gagtcgttag aactaggctg tgattactat ataggggttt atccagaaac actagcaagc
     7561 gaatttcatc gtcctatctt gcccctttat cgggtcaacg cttttgaaag cagggataga
     7621 gaagttcttc aagttttaac agcgattaaa gaaaatctac cgctcagaga agttcccttg
     7681 cgttcgagac aagatgtctt tatatcagca agttctttag agaaattatt ccaagaacgt
     7741 tttcctcaag ccttggaaaa cttagaaaag cttatttcag gtatttctta cgatttggat
     7801 actagtttga aactgcctcg ttttaatcca gctagaccag cagtagagga gttgagagag
     7861 cgtgctgaac tggggtttgt tcagaagggg ttgactagta aagaatatca agatagacta
     7921 gaccaagaat tgtctgttat tcatgatatg ggctttgatg attatttctt ggttgtttgg
     7981 gatttgttgc gttttggacg atcgaatggc tattatatgg gaatgggaag gggttctgca
     8041 gtaggcagtt tggtttctta tgccttagac atcacgggga ttgacccagt agagaaaaat
     8101 ctgatttttg aacgctttct taatcgtgaa cgctatacca tgccagatat tgatattgat
     8161 atcccagata tttatcgtcc agattttatc agatatgttg gtaataaata tggtagtaaa
     8221 catgcggcac aaatcgttac tttttcaacc tttggagcca agcaagctct acgagatgtc
     8281 ttgaaacgct ttggtgttcc tgagtatgaa ttatctgcaa ttactaagaa aatcagtttt
     8341 cgtgacaatc ttaagtcggc ctatgaggga aatctccagt ttcgtcagca aatcaatagt
     8401 aagttagaat accaaaaagc ttttgagatt gcttgcaaga tagagggcta tccaaggcaa
     8461 acctctgtcc atgcggctgg tgttgtaatt agtgaccaag atttaaccaa ctacattcct
     8521 ctaaagtatg gtgatgaaat tccactgact cagtatgatg ctcatggagt tgaggctagc
     8581 ggacttttga agatggactt tctgggacta cgaaatttga cctttgttca gaagatgcaa
     8641 gagttgcttg ctgaaacaga aggtattcat ctgaaaattg aagaaatcga tttagaagac
     8701 aaagaaacgt tagctttatt tgcctctggt aatacaaaag gtatctttca atttgagcaa
     8761 ccaggtgcca ttcgtctgct taagcgtgtg caaccagtct gttttgaaga tgtcgtcgcg
     8821 actacttctc taaatcgacc gggtgctagt gactatatca ataattttgt ggcaagaaag
     8881 catgggcagg aagaagtgac tgttctggat ccagtactgg aggatatttt ggctccaacc
     8941 tacggcataa tgctctatca ggagcaggtt atgcaggttg cccagcgatt tgccggattt
     9001 agtcttggga aagccgatat tttgcgtcgg gctatgggga aaaaggatgc ctctgccatg
     9061 catgagatga gggcttcctt tattcaaggt tcattagaag ctggtcatac tgtggaaaaa
     9121 gcagagcagg tctttgatgt tatggagaag tttgcaggtt atggttttaa caggtcacac
     9181 gcctatgcct actcagcctt ggccttccag ttggcttatt ttaaaacaca ttatccagcc
     9241 attttttatc aggtcatgtt aaattcttcc aacagtgatt acttaataga tgcacttgaa
     9301 gcaggttttg aagtagcctc tctatccatc aacaccattc cctatcacga taaaattgcc
     9361 aacaaggcca tctatctagg tttgaaatcc attaaaggag tcagtaatga tttagctctc
     9421 tggattattg aaaatagacc ttattctaac attgaagatt ttatagctaa attacctgag
     9481 aattatctga aacttcctct gctagaacct ttggtaaaag ttggtctttt cgattcattt
     9541 gaaaaaaatc gtcaaaaagt atttaataat ttagctaatc tatttgaatt tgtgaaagag
     9601 ttgggaagtt tgtttggaga tgctatttat agttggcagg aatcggaaga ttggacggaa
     9661 caagaaaaat tttatatgga acaagagctt ttagggatag gtgtcagcaa acatccacta
     9721 caagctattg caagtaaggc tatttacccg attaccccaa tcggaaattt gtcagaaaat
     9781 agctatgcta ttatcttggt tgaagttcag aaaataaaag tgattcgtac caaaaagggt
     9841 gaaaatatgg ccttcttaca ggcagatgat agtaagaaaa aattggatgt cactctcttt
     9901 tcagacttat atcgtcaggt tggacaggaa ataaaagagg gagccttcta ctatgtaaaa
     9961 ggaaaaatac aatcacgtga tggccgtctg caaatgattg cacaagaaat aagagaagca
    10021 gttgctgaac gcttttggat acaggtgaaa aatcatgaat cggatcaaga aatttcacgc
    10081 attttagaac aatttaaagg cccaatccca gtcatcatcc ggtatgaaga ggaacagaaa
    10141 accatcgttt ctccccatca ttttgtagct aaatccaatg aattagagga gaaattgaat
    10201 gaaatcgtta tgaaaacgat ttatcgctaa aaatacggaa aatagaagaa ttttaaaatc
    10261 aaatgtgata taatcaagaa gaatgttaaa agaaaaagga gcataaacca atatgaaacg
    10321 tattgctgtt ttgactagtg gcggagacgc acctggtatg aacgctgcca tccgtgcagt
    10381 tgttcgtcaa gcaatttcag aaggaatgga agtgtttggt atctatgacg gatatgctgg
    10441 tatggttgcc ggtgaaattc atcccctaga tgcagcttca gtaggggaca tcatttctcg
    10501 tggtggtact tttcttcact cagctcgtta tccagagttc gctcaacttg aagggcaact
    10561 taaagggatt gagcaattga aaaaacacgg aattgaaggt gtagttgtta tcggtggtga
    10621 cggatcttac cacggcgcta tgcgtttgac tgaacatggc ttcccagcta ttggtcttcc
    10681 aggtacaatc gataacgata tcgttggtac tgactttaca atcggttttg acacagcggt
    10741 tactactgcc atggacgcta tcgataagat tcgtgataca tcatcaagtc accgtcgtac
    10801 ttttgtaatc gaagttatgg gacgtaacgc tggtgatatc gctctttggg ctggtattgc
    10861 aactggtgct gatgaaatca tcatccctga agcaggcttc aagatggaag atatcgtagc
    10921 aagcatcaaa gctggttatg aatgtggtaa aaaacacaat atcatcgtct tagctgaagg
    10981 tgtgatgtca gcggctgaat ttggtcaaaa acttaaagaa gctggagata caagcgacct
    11041 tcgtgtaaca gaacttggac atattcaacg tggtggttct ccaactgcgc gtgaccgtgt
    11101 tttggcgtca cgtatgggtg cacatgctgt taaacttctt aaagaaggta tcggtggtgt
    11161 tgcggttggt attcgtaacg aaaaaatggt tgaaaatcca attcttggta ctgcagaaga
    11221 aggggcattg tttagcctta ctgcagaagg taagattgtg gttaacaacc cgcacaaagc
    11281 cgatattgag ctatctagct tgaataagag cttgtcataa tttacaattt agttaataag
    11341 accaaaaagg tcatatatat aaaggagtca caaaaatcat gaacaaacgt gtaaagatcg
    11401 ttgcaacttt gggtcctgcg gtagaaatcc gtggtggtaa aaaattcggt gaggacggat
    11461 actggggtga aaaacttgat gttgaagctt cagctaaaaa cattgctaaa ttgattgaag
    11521 ctggtgctaa cacattccga ttcaacttct cacacggcga ccaccaagaa caaggtgagc
    11581 gtatggcaac tgttaaactt gcggaaaaaa ttgcaggtaa aaaagttggt ttccttcttg
    11641 atacaaaagg acctgaaatc cgtacagaat tgttcgaagg tgaagctaaa gaatattcat
    11701 acaaaactgg tgaaaaaatt cgtgttgcaa ctaaacaagg aatcaaatca actcgtgaag
    11761 tgattgcgtt gaacgttgct ggtgctcttg atatctatga tgatgttgaa gttggtcgtc
    11821 aagttttggt tgacgatggt aaacttggtc ttcgtgtggt tgctaaagat gatgcaactc
    11881 gtgaatttga agttgaagtt gaaaacgatg gtatcatcgc taaacaaaaa ggtgtgaaca
    11941 tccctaacac taaaattcct ttcccagctc ttgctgaacg cgataacgac gatatccgtt
    12001 tcggtcttga acaaggtatc aacttcatcg caatttcatt cgtacgtact gcaaaagatg
    12061 tgaacgaagt tcgtgcaatc tgtgaagaaa ctggaaacgg acatgttcaa ttgttcgcta
    12121 aaatcgaaaa ccaacaaggt atcgataact tagacgaaat catcgaagca gctgatggta
    12181 ttatgattgc tcgtggtgat atgggtatcg aagtaccgtt cgaaatggtt ccagtttatc
    12241 aaaaaatgat tatcaagaaa gtcaatgctg caggtaaagt tgttatcact gcaacaaaca
    12301 tgcttgaaac aatgactgaa aaaccacgtg caactcgttc agaagtatca gacgtattca
    12361 acgctgttat cgacggaact gacgctacaa tgctgtcagg cgagtctgca aacggtaaat
    12421 acccactcga gtcagtaact acaatggcta caatcgacaa gaacgctcaa gctcttctta
    12481 atgaatacgg acgtcttgat tcagattcat ttgagcgtaa ctctaagaca gaagtaatgg
    12541 cttctgctgt taaagatgct actagctcaa tggatatcaa attggttgta actcttacta
    12601 agacaggtca tactgcacgt ttgatttcta aataccgtcc aaatgctgac atcttagcat
    12661 tgacatttga cgaattgaca gaacgtggct tgatgttgaa ctggggtgtt atcccaatgt
    12721 tgacagatgc tccatcttca actgacgata tgttcgaaat cgctgaacgt aaagcggtag
    12781 aagcaggtct cgttgagtca ggcgatgata tcgttatcgt tgctggtgtg ccagtaggag
    12841 aagctgttcg cacaaacaca atgcgtatcc gcacagtacg ttaagaaaaa tataaaaacc
    12901 tatcatatcc agctttctag cctgtatgat gggctctttt tttgtccagg gggcaaaaag
    12961 ggggcaaaac tttcataatg gattgatact cttcgaaaat ctcttcaaac cacgtcagcg
    13021 tcgccttacc gtatatatgt tactgacttc gtcagttcta tctacaacct caaagcagtg
    13081 ctttgagcaa cctgcggcta gcttcctagt ttgctctttg attttcattg agtatgaaat
    13141 aagatatgca caaattgatt agaaagtcaa atgaatttct acaaatgttt tagcaatcgc
    13201 aatctacttg tctagattcg atctgatata ttttcgattt aatgatatgg tatttaaaac
    13261 ctccaaagta gcttgctcca ttcttttact tacgtgagtg tagatgttat ttactgtttt
    13321 agcgtttttg tgttccactc taaccattat agcattcttc tcagctagtg tactaaggag
    13381 tatgtgcctg aaaatatggg aactaagggg ctggtttatc ggtttctcta gtttagtatt
    13441 tgccttttgc aaagtgatgt taaatgcctt tctctaaatt tacatatcac tattgtttaa
    13501 caaaatctaa tctactttag gtcacttatt ctttttttga aatgtagaat gaactttttc
    13561 aaagtttttc gaatctttta aaatctgttt gctttatatc gccattctcc ccctttttta
    13621 attctcccta tatagcctga cagctttccc gatggtacga atatggttgc tttcgtctag
    13681 gtggatgtcg gggtattcgg gattgagttt ttttgaggca gccttggcgg agtttcttga
    13741 catagttagt gccgtctact tggaagatgc cgatggtatt atagtcaatc tgtggggtat
    13801 tcttgataaa taggtagtcg ctgtttctta tctttggctc catggacttg ctgacgacat
    13861 aagcgattgg gtcgtagtcg tctgggataa tggaaactcc ataactaaat cgtcgtcctg
    13921 catcgagcgg ctacctgcag agataaacta cctaacacga gagtaagtag tctgtctgta
    13981 gtcgtccagt ctgatgattt ttacgatatt tcgtttttct gctcatacag ttgcctctcg
    14041 gcataggtca ggactttacc ttgtctgggt ggtgcccgtt ggtcgtagat agattggata
    14101 tcgctaggag aatccttttg aactggagga aagagggtat caatcaagct actgaatact
    14161 ttagctaagt caaatatagt atttttctta gtagacctaa cccttttttc ataatttcca
    14221 atggtgtttt tacttatacc aatcttagta cccaattctt attgagtcca accattacta
    14281 gtctatattg ttttatagtt gattgagttt ggaatagtac gctgtagctg ctaaaacatt
    14341 tctaggaatt aatttgactt tcctaataga gttgttcata tcttatttca atctactatg
    14401 tttttcacct ctaacaatcg caatctcttc tttatccatg aatgaaatcg ctttctattt
    14461 ttgtaagtaa agcatcacac gaaatccacg aaaatgaaaa cctttgttgt gttttcgtaa
    14521 aaaatttgtt gacagagcac gaaacgccta ctataattaa atcaagctta aggaaataac
    14581 aaaaaaacaa atcggaggga aagacgatga actcattaaa tgagaaagta atcaatatct
    14641 gtaaagcagt agttaaagaa actttaatcc aagacattta ggagaaagaa atgaagaaat
    14701 caagaaaatt agccacatta ggaatctgtt cagccttgtt tttaggcttg gctgcttgtc
    14761 aacaacaaca tgctacttct gaggggacga atcaaaggca aagcagttca gcgaaagttc
    14821 catggaaagc ttcatacacc aacctaaaca accaggtaag tacagaagag gtcaaatctc
    14881 tcttatcagc tcacttggat ccaaatagtg ttgatgcatt ttttaatctc gttaatgact
    14941 ataataccat tgtcggctca actggcttat caggagattt cacttccttt actcacaccg
    15001 aatacgatgt tgagaaaatc agtcatctct ggaatcaaaa gaagggcgat ttcgttggga
    15061 ccaactgccg tatcaatagc tattgtcttt tgaaaaattc agtcaccatt ccaaagcttg
    15121 aaaagaatga ccaattgctt ttcctagata acgatgcgat tgataaagga aaggtctttg
    15181 attcacaaga taaggaagag tttgatattc tattttcgag agttccaact gaggcaacta
    15241 cagatgtcaa ggttcacgct gaaaagatgg aaacattctt ctcacaattt caattcaatg
    15301 aaaaagctcg aatgctgtct gtagtcttgc acgacaatct ggatggcgag tatctgtttg
    15361 taggccacgt tggggtctta gtacctgctg atgacggttt cttatttgta gagaaattga
    15421 ctttcgaaga gccctaccaa gcgattaaat ttgctagtaa ggaagattgc tacaagtatt
    15481 taggcaccaa gtatgcggat tatacaggcg agggactggc taagcctttt atcatggata
    15541 atgataagtg ggttaaactt taatagtgtt atgatatgac agtaatctta ggatttttat
    15601 ttctattttt gatgagttgg tgggttattt caatcgttaa tagccagaaa ccacgcaaaa
    15661 atgaaacatt tattggctat atccaaagat acgatattga tgggaatggc tatcaatcat
    15721 taccgtgtcc tcagaactga gaggagttct tgaaatcgta aaactacttt gaacaagagt
    15781 tacttcaacc cattggctcc gacggattaa gttgctttcg tgaacaccaa aatcagccgc
    15841 aatttcttca taagtgcggt attctcgcac atattgaaga gtggccataa gaaggtcttc
    15901 taggcttaat ttaggttttc gtcccccttt tgtgtgttta agttgataag cagtttttaa
    15961 tacagctaac atctcttcaa aagtggtgcg ctgaacacca acaagacatt taaatcgtgc
    16021 atcagttagt tgcttacttg cttcataatt catagaacta ctataccata ttttgtttcg
    16081 caggaagtct aatgctaaaa tcttatcagt gagaagattt cttaaagttt attctttgta
    16141 gtttcatctg tattagtaca taatttaact tagagaaaat aggaggacta atcgaatgga
    16201 attaaaagat tttacagaaa aagaacagga aatgattaag aaaaggctta caatgtctaa
    16261 tattagtgac aaagaaacta ctgagaagat tctggcgcta gtaccacaag acttgattaa
    16321 gcgaatcccg ttttttgtca gaaaacatgc tacaacacgt acgattaaac gcatttcaat
    16381 tgaacaccct gaactctacg ctgtagctca aacaagtggt gagattccag aaaaaaaaac
    16441 gcgaagaatt gcgtcagatt atcacaacta tctttgaaca aaagatgaat aagcatagta
    16501 ttaagtagag aatgaaaaaa tattttattg gcggtttggg aagcaatgcc tatcatagca
    16561 aggattttct ttaagaacta gattcgcagg tctattttct aaatccatat gaaaagcatc
    16621 ttcgagatga aacagaattg aaatcatggt ttaaaaatga gattgtagag gaagaatcta
    16681 tctgtctgat aggtcattct attggaggcg atttagctcg ttatttcgca tcggaatttg
    16741 aagaagtaaa gaaactgatt cttttggatg gtggctatct agatttagat aagattttac
    16801 ctttggatac agagttagag gaaactaaaa attatatcaa atctcaaatt atttcggact
    16861 tagctcttct tacttctaaa gaaaaatctg aagcaaagca ttggtcagaa aatatggaga
    16921 aagctgtaag acagtcctat cactggaatg ttgagtataa tagatatgag ttggctataa
    16981 attatgaaaa tatagaagcg atactccgcc tacggagaaa aatacaagct tttaagagag
    17041 aagtgggaga taccttgttt attagtcctc gctatcctaa tgaagctaca tggagagagg
    17101 aagccctaaa agaattgcca gactattttg atactatttt tttagaaaac tttggccatg
    17161 agctttatac tcaagcacct aaagaaatcg ctagtctaat gaatgagtgg ctcgcttatt
    17221 ttctatgagc tacttgtatt gctggaaatg gtataagatg aaaatctaca agtcactaat
    17281 tttcatataa ccaaaaaacg aacaggctct ttttctgtta ttcagaagag aacttgttcg
    17341 tttttatctt gattttctta aaaagctcta tatacatagg gattttctgg tttatcgact
    17401 ttggtaaagc tgtcgatttc caaggttgga aggttttgtt tgagttcttt atggtagtga
    17461 ttgaccagtt ttcctttggc tgaaatccaa gtgttgtttt catactgccg ggtattgccc
    17521 ttggtcagca atccatagac accagaatct gcgatacagt ggataatgcc gaatcggaac
    17581 agaaattgac tattggcatg actggggtcg ttatagacaa agcctgtaaa ctggattgtc
    17641 ttgccctcaa actcatctgg atagtcgtag atagcctcca tgacttccat atagttttca
    17701 ttagtgatct gaatactatc ttgggataag tatttatccg ccgccgttcg catttccttt
    17761 tcataggctg attttgaaaa ataagaactg gtatctggtt tcaaatattg gcttgtcgtc
    17821 ccttcgcttg tctgaatggc tagatccgtt ccttccgata gggggaaatg ataaccttta
    17881 gcagaaacag tctgagaatc caagctaaca gttgggaaag ttaagccgat gacaagtgga
    17941 atagccagaa gagaaatact cgtcatcttg gccaatcggc tgttcagatg actgtgggtt
    18001 ttgacttgct tcatccagat atacaattga acaattgcca agataaagga aagcaccatg
    18061 gaaatatagg ccagatagga atagtgcatg ttgatgtact ggtttagttt gcccgacaga
    18121 tggaggtaaa tagtcagttc aaaatagcca gctaaaacta aaaatcgaat cataggatga
    18181 ctccaatcaa gagagaatag gctaaaacga ccagagtcac aatcgtaatg aagtgactaa
    18241 taaatcgtgc tttcaagtaa tttttcatca tgagaatatt tttgatatcc agcattgggc
    18301 caatgacgag aaaggccaga actggagcca aaccgaaact cgagagtaga gaagcaccta
    18361 taaaggcatc cgcctcacta cagagcgaaa gaagaaaggc caaaagcatc aagagcagga
    18421 tggcaaaaag aggagtcgca ctgatagagg tcagaatccg agtcggaacg tagacctgta
    18481 ttatagaagc aaagaggcag ccaaatacca aataacgccc cgtatcaaaa aattcatcaa
    18541 tggcctgcac aaagacttga aaaacttttt ttgcagaact caagtaagaa aaatcatgct
    18601 catgacaagc cagacgattt tctttctgaa tcggttcttg ccagaaaaat cctagaaaaa
    18661 ttcctagtat tacagccaca agaatggaac ccagagctcg taatagggcg acatggaagg
    18721 agttgccaaa ggcagaatag gtcgcaaaaa gaacaatggg attgataaca ggtgctgtca
    18781 caagaaaagg aactgccgtg taacttggaa cctttttttc cagaaaacga ttgatgatgg
    18841 ggacgattcc acattcacaa gaagggaaaa gtataccgac aaaggtccca aaaaagattc
    18901 tcccccaacg atttcgaggg agaaaatgat aaaccttgtc aggtgtgata taaacttcaa
    18961 tcagccctga gacaatgctt cctatcagaa cgaagggaag ggcttcaatg ataatggaga
    19021 gaaaaatggc tccagtttga agtacactag atgggagttg ttgaaaagca gtcatctagt
    19081 ttttcttttc tgctttgact tttttctctt tgtcatcagg gaaagtaact tgtttcaaat
    19141 cagggagttt agcaaactct tcaaacatct tgtccaagtc gtctcgtttt gtaaatttga
    19201 cagccatagg tgtacctcga ttctaaattc tacctctatt atactattat tttgcggaaa
    19261 agggagtttt ttaatttgtt ttcgatagtt gatacacttc gaaaatctct tcaaaccacg
    19321 tcagcttcgc cttaccgtag tacggttact gacttcgtca gttctatcca caacctcaaa
    19381 acagtgtttt gagctgactt cgtcagtctt atccacaacc tcaaaacagt attttgagca
    19441 gcctgcggct agcttcctag tttgcttttt gattttcatt gagtatgaaa aggattggag
    19501 aaagccttcc agcttgtggt ataatagatt tatggataaa aaatatgaaa aaatctctca
    19561 ggatttggga gtgacgttaa agcaaattga taccgttcta agtttgacag ctgaaggggc
    19621 gactattccc tttatcgcgc gttatcgcaa ggacatgact ggtagtctgg atgaggtggc
    19681 gattaaggct attattgatt tggataaaag tctgacaaat ctcaatgacc gtaaggaagc
    19741 tgtcttagct aagattcaag aacaaggtaa gttgaccaag gaattggaag aagctatctt
    19801 agttgccgaa aaattagcag acgttgaaga actctatctt ccttataagg aaaagcgtcg
    19861 taccaaggca accattgccc gtgaagctgg actctttcct cttgctcgtt tgattttgca
    19921 gaatatagtt gacttagaga aagaagctga aaagttcgtc tgtgaaggat ttgcgactgg
    19981 caaggaagcc ttgactggtg cagttgatat tttggtcgaa gccttatcgg aagatgtgac
    20041 cttgcgttct atgacttatc aggaagtgct gagacactct aaactcactt ctcaagccaa
    20101 ggatgaaagt cttgatgaaa agcaggtttt tcagatttat tatgattttt cagagacagt
    20161 tggaactatg caaggctatc gtaccttggc tctcaatcgt ggggagaaac ttggtgtctt
    20221 gaagatcggt tttgaacatg cgacggaccg tattcttgcc ttctttgcta ctcgtttcaa
    20281 ggtgaaaaat gcttatattg atgaagttgt tcagcaatcc gttaagaaaa aggtcttgcc
    20341 tgctattgag cgtcgtattc ggacagaatt aactgagaaa gctgaagagg gagctatcca
    20401 acttttttct gacaatctgc gcaatctcct cttggttgct ccactgaaag ggcgcgtggt
    20461 tcttgggttt gacccagcct ttcgtacagg tgccaagcta gctgtcgtgg atgcaacagg
    20521 aaaaatgctg acaactcagg ttatttatcc tgttaaacca gcatcagctc gtcaaatcga
    20581 agaagccaag aaagatttag cagatttaat tggtcaatac ggtgtagaga ttattgccat
    20641 tggaaatgga acggccagtc gtgaaagtga agcttttgta gcggaagttc tgaaagattt
    20701 ccctgaagtc agctatgtta tcgttaatga aagtggtgct tctgtctatt ctgccagcga
    20761 acttgctcgt caggagtttc cagacttgac cgttgaaaaa cgctctgcca tttctatcgc
    20821 ccgtcgtttg caagatcctc ttgcggaatt ggtcaaaatc gatcctaagt caattggtgt
    20881 cggtcaatac caacacgatg tcagtcagaa gaaactatct gagagtctgg actttgttgt
    20941 cgatacagtg gttaaccaag ttggtgtcaa tgtcaataca gctagcccag ctcttctttc
    21001 acacgtagct ggactcaata aaaccatttc tgaaaatatc gtcaaatacc gtgaggaaga
    21061 gggaaaaatc acttcacgct cccaaatcaa gaaagttcct cgtttggggg ccaaggcttt
    21121 tgaacaggct gctggtttcc tccgtatccc tgaaagtagc aatatccttg ataatacagg
    21181 agttcaccca gaaaattacg ctgccgttaa ggaactcttc aaatgcttgg acattaagga
    21241 tctgaatgaa gaggcccaaa gcaaactcaa gtcgctttca gtcaaggaaa tggcgcaaga
    21301 gttagacctt ggtccagaaa ctcttaaaga tatcattgca gatcttctca aaccaggtcg
    21361 agatttccgt gatttctttg atgctccagt tctccgtcaa gatgtcctag atatcaagga
    21421 cttagtggtt ggacaaaagc tagaaggagt ggtgcgtaac gttgttgatt tcggtgcttt
    21481 tgttgatatc ggaattcatg aggacggtct catccatatt tcccatatga gtcgcaagtt
    21541 tatcaaacac cctagtcaag tggtggcagt tggagatttg gtgagcgttt gggttaagaa
    21601 aattgatact gaacgtgaaa aagttaatct gtctctccta gctccaaatg aaactgactg
    21661 attacgtcaa gcaggtttca ctagaagact tcggcagacc ttttatccat catgtccagt
    21721 ggaataggcg tctacgttcg acaggtgggc gatttttccc caaagatggg catttggatt
    21781 ttaatcccaa ggtttatcag gaactggggt tggacgtttt taggaaaatt gtccgacatg
    21841 aactctgtca ttatcacctc tattttcaag ggaaaggcta tcaacataag gatcgggatt
    21901 ttaaggaact gttgaaagca gtggatggat tacggtttgt accatccttg cccaatagta
    21961 actccaagcc aatcaagctc tatcgttgcc aatcctgcca gcaaacttat cagcgtaagc
    22021 gaagaattga taccagacgc tatcgctgtg gactttgtcg aggtaaattg ctactaataa
    22081 atcagcctga ggactgatga aagccgagcc tacccgtgat atactatgtc taacctagca
    22141 gaaagaggaa atatcatgaa cagtaagttt tataaaatga gacgaaatcg catgatttca
    22201 ggagttttag caggtctgtc agacaagtgg aactttgatg ttacccttgt acgttttctg
    22261 tttgccattt ttacagtcgc aaactttgga cttggggtga ttatctatat catcctagct
    22321 tctatcatgc caaccaagga agaaatcgaa gcagaaatgt acggaacagg cccacgtaaa
    22381 cgcaaagaag ctcaagccat tgacgataat gaaggctggt tttggtgagg ttttacaaaa
    22441 gatttggaat acgcaaaaag ctttaaatca atagtctaaa ctgcttgatt taaagctttt
    22501 ttacttttat actcaataaa aatcaaagtg caaactagga agccagccgc aggctgtaat
    22561 ttagtcggca atgtgaagtt gacatagttt gcactttgat ttttgaatag tattaataat
    22621 atttcgtcag cttcatccac aaactatagt ggattg
//
LOCUS       DAVBGY010000030        22217 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVBGY010000030 DAVBGY010000000
VERSION     DAVBGY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22217
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.030"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(71..2029)
                     /gene="ftsH"
                     /locus_tag="VQ620_001184"
     CDS             complement(71..2029)
                     /gene="ftsH"
                     /locus_tag="VQ620_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET2763809.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNREIHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            complement(2045..2587)
                     /gene="hpt"
                     /locus_tag="VQ620_001185"
     CDS             complement(2045..2587)
                     /gene="hpt"
                     /locus_tag="VQ620_001185"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET2763810.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKAREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            complement(2591..3868)
                     /gene="tilS"
                     /locus_tag="VQ620_001186"
     CDS             complement(2591..3868)
                     /gene="tilS"
                     /locus_tag="VQ620_001186"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET2763811.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVENLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            complement(3865..5133)
                     /locus_tag="VQ620_001187"
     CDS             complement(3865..5133)
                     /locus_tag="VQ620_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HET2763812.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSSSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            complement(5253..5621)
                     /locus_tag="VQ620_001188"
     CDS             complement(5253..5621)
                     /locus_tag="VQ620_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET2763813.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            complement(5614..5880)
                     /locus_tag="VQ620_001189"
     CDS             complement(5614..5880)
                     /locus_tag="VQ620_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET2763814.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            complement(5948..9457)
                     /gene="mfd"
                     /locus_tag="VQ620_001190"
     CDS             complement(5948..9457)
                     /gene="mfd"
                     /locus_tag="VQ620_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET2763815.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYAQHAFIHQLKENG
                     YQKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPIQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            complement(9458..10027)
                     /locus_tag="VQ620_001191"
     CDS             complement(9458..10027)
                     /locus_tag="VQ620_001191"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET2763816.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVKYYLQEKNFEKTMQRYNG"
     gene            complement(10098..11213)
                     /gene="ychF"
                     /locus_tag="VQ620_001192"
     CDS             complement(10098..11213)
                     /gene="ychF"
                     /locus_tag="VQ620_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET2763817.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            complement(11297..11491)
                     /locus_tag="VQ620_001193"
     CDS             complement(11297..11491)
                     /locus_tag="VQ620_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HET2763818.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(11556..12692)
                     /gene="dnaN"
                     /locus_tag="VQ620_001194"
     CDS             complement(11556..12692)
                     /gene="dnaN"
                     /locus_tag="VQ620_001194"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET2763819.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            complement(12851..14212)
                     /gene="dnaA"
                     /locus_tag="VQ620_001195"
     CDS             complement(12851..14212)
                     /gene="dnaA"
                     /locus_tag="VQ620_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET2763820.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(14425..15183)
                     /locus_tag="VQ620_001196"
     CDS             complement(14425..15183)
                     /locus_tag="VQ620_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET2763821.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(15241..16422)
                     /locus_tag="VQ620_001197"
     CDS             complement(15241..16422)
                     /locus_tag="VQ620_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET2763822.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            16605..17084
                     /gene="rlmH"
                     /locus_tag="VQ620_001198"
     CDS             16605..17084
                     /gene="rlmH"
                     /locus_tag="VQ620_001198"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET2763823.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            17128..17201
                     /locus_tag="VQ620_001199"
     tRNA            17128..17201
                     /locus_tag="VQ620_001199"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17162..17164,aa:Arg,seq:tct)
     gene            17366..17491
                     /gene="comC"
                     /locus_tag="VQ620_001200"
     CDS             17366..17491
                     /gene="comC"
                     /locus_tag="VQ620_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HET2763824.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            17512..18837
                     /locus_tag="VQ620_001201"
     CDS             17512..18837
                     /locus_tag="VQ620_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2763825.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            18834..19586
                     /locus_tag="VQ620_001202"
     CDS             18834..19586
                     /locus_tag="VQ620_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2763826.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            19638..19709
                     /locus_tag="VQ620_001203"
     tRNA            19638..19709
                     /locus_tag="VQ620_001203"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19671..19673,aa:Glu,seq:ttc)
     gene            19715..19788
                     /locus_tag="VQ620_001204"
     tRNA            19715..19788
                     /locus_tag="VQ620_001204"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19749..19751,aa:Asn,seq:gtt)
     gene            complement(19829..20371)
                     /locus_tag="VQ620_001205"
     CDS             complement(19829..20371)
                     /locus_tag="VQ620_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET2763827.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            20550..21317
                     /locus_tag="VQ620_001206"
     CDS             20550..21317
                     /locus_tag="VQ620_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HET2763828.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            21402..22082
                     /locus_tag="VQ620_001207"
     CDS             21402..22082
                     /locus_tag="VQ620_001207"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HET2763829.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFVAAISTNMIFAKLPSGRH
                     PESRWAWLKSRAEINGIIAVLAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMV
                     TTLTTWNSRIGAFFLLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQT
                     ISINKSFS"
BASE COUNT         6868 a         4091 c         3634 g         7624 t
ORIGIN      
        1 cattttctcc attctgttcc ctttagctcc taaactgcac aaaaaagaga ggctccagcc
       61 tctctcaggg ttatttttcg tcattcattt ttgactttac ttcatcatag gatagtgcat
      121 gagattcctc ttctactgct tcaggcatct ttcctgtttc gtaaagagct ttaatttgtg
      181 tactatccaa tgtttcgtat ttcaataatg cttctgcaat taacttgtga atttcacgat
      241 ttgactgaat aatttcagca gctttatttc gtgcctcatt taataatgaa cgaacctctt
      301 catcaatttc ataagctgtt tgttctgaaa ttgatttttg aggactctgt gcaccaaaca
      361 tagcatggtt tccttcatat tgtactgggc caagtttttc actcataccg tactctgtaa
      421 ccattgcacg cgccatttgt gtcgcttgtt caaagtcgtt tgaagctcct gtagtttgga
      481 cattaaagat aatttcttca gctacacgtc cacccattaa gccagccaat tgctctttca
      541 tatcttcttt agatagaagc atttgatcct ctttaggaag tgcaatcatg tatccgcctg
      601 cacggccgcg tggtacaatt gtaaccttat ggacaacgcg agcattcgac aagactagac
      661 caacaatggt atgtcctgcc tcatggtaag caaccaattc tcgttctttt tgtgaaactg
      721 tcttatcttt cttagaaggt ccagcaataa ctctatcttc tgcttcatca atatctgaag
      781 catcaattat cgatttattg cgacgagcag caactaaagc tgcttcattc aagacattct
      841 ctaaatcagc accaacaaag cctggagttt gttgagccac taatttcaaa tcaacatctt
      901 ctgctaaagg cttgttctta gcgtgaactt tcaagattgc ttcacgacct ttaacatcag
      961 gacgaccaac caatactttt ctatcaaaac gtcctggacg caaaagggca ggatcaagta
     1021 catctgaacg gtttgtcgca gcgatgacga taatcccttc atttccctca aaaccatcca
     1081 tctcaatcaa aagttggttc aaggtttgtt cacgttcgtc attacctccg ccgagaccga
     1141 ctccacgttg acgtccaaca gcatcaattt catcgataaa gatgatagct ggtgctgctt
     1201 ttttggcatc ctcaaaaaga gagcgaacac gactagctcc aactccgaca aacatttcta
     1261 caaagtcaga acctgagata ctaaagaatg gaacacctgc ttctccagcg actgccttag
     1321 caagcaaagt tttacctgtc cccggaggtc cctccaaaag aacacctgct ggaatacggg
     1381 ctccaagttt tgtgaatcgt tttggatctt ttaagaactc aacaacttca actagttctt
     1441 gtttttcttc ctcagctcca gcaacatctg aaaatcttac tttaatatct tctttatttg
     1501 ctgctttagc cttactacgt ccaaaactca ttggattacg gccattgcct cctcccatat
     1561 ttcccatcat agagaatagg aagaagaata gaattccaaa tggcacaatg gatacgagta
     1621 gattaatcca tataccactt gaactttcat gcttaacagt tacttctgct ttatggtcag
     1681 tagcaagttt ttgcaattct gatacggtag tatctgcagg aagaatagtg ctggtaaatt
     1741 tctctacctt agtaacagat ggcgtgaaaa actgaatacc tgttccttct ttacttgttt
     1801 taggattttt atagacacca gaaacttcga taacactacc atttggttgg taagttaatt
     1861 cttttacatt accatcggta atttcttgta ccaactcagt atagttgatt tgctgacttc
     1921 ctcctgagtt attcccagaa tagaaatact ggaatcctgt cacaaggaaa aagataaata
     1981 ataaccatag aaaaggattt ttaattaaac cattattttg ttttttcatt aaagattatt
     2041 ctttctaatt tgaatacact tcctctttca atactccaat ataaggaaga ttacgataat
     2101 tttctttgta gtctaaacca taacctacta caaactcatt tgggatagta aagcaggtat
     2161 agtctgcctc aatttctaca acacgtcctt ctggtttatc caacaaggtt gcaattttaa
     2221 cagaagctgc ttctcttgct ttaaacatat ctcgcaaatt cttcaaagtt tgacctgtat
     2281 caatgatatc ttctacaaat agaacatgtc ttcctttgat atcttgagtc acatcttgtt
     2341 taatattgat aacaccacta cttgctgttc caccatggta gctagaaacc atcatgaagt
     2401 ccatttcaat atgtgtatca atatgtttga ccaattcagc cataaaagga atagatcctt
     2461 ttaaaatccc aactaagatt ggattttttc ctgcatagtc tttagttaat tgagcaccta
     2521 gttttttagc tgcttctgta atttcatcgt gtgaaacgag gactttttta atatcgtttt
     2581 ctaacatttt ttacctatct attttttcta tataaagtac agtgttcatt atatcatttt
     2641 tcgttttttt actcaaatta ttggtcgcaa ttcccaaaat tgagacaatt tcaccaaatt
     2701 gctcaataat aagagcagag tttctctttt ccatagggat tttcaaatca ataaataaac
     2761 gtctgagttt ttttctatgc ccattttgaa tcaaaacatc tcctgttttt cgatgacgaa
     2821 tgtgtatgga tgtttcacgt gaaacaggta tttgttgaat taattcacct tctaatggaa
     2881 gtccaaagga aaataaatat ccttgataag ctacctgatt ttgatagtgt aacacaagtt
     2941 catcttcctc ttcatcagcc tgcggactga ttttacaaat ctgaaactgt tggtactctt
     3001 ttatcaattc atagccattt ttaatcggat gacgatactg gcttttagat tttaaaatct
     3061 gctgaacttc agcaaactga gcttttgtaa gattcaaatc tggaaaacga ttcagataag
     3121 tttgaagtaa aactctttgt gtagactcag agtaagaaaa taactgctgt aaattttcca
     3181 cattaatatt gttagataat tcagctattg ccaaatcata atctaaaatt tcattgccga
     3241 tgcctaagat tgcatcccta aatcgaggat tttctttttc caattctggt aagtaagaat
     3301 ttcgaatacg atttcgaaaa taatgattct cctgatttga tgtatcttca aagtgaaaaa
     3361 ttgatggaaa gtcttttttc tgaaaatgca agaagggacg aatgatttct atctctccga
     3421 ctacttgctt ctccttaatt cctgatagat agcgcaagcg agttcctcga atcaagcgca
     3481 tcaaaatcgt ttccacctga tcatcagcat ggtgggcagt gactaaagct gtcgcacctg
     3541 tctttttcat gacctcttga aaaaaatcat aacgaaaatt tcgtgcacgc gcttctgaaa
     3601 attctcctga aaaattgctg atataaatag gaagctctgc ttcagcagcc aacttcctta
     3661 attccttttc ttcccaatct gattcaattc tctgcttatg attcacatga gctagaatca
     3721 attcaatctc taactctttt tgataagtag acaatacctt aaatagaaac atggaatcta
     3781 atccaccaga aagagctaga accgccttag catgcttttt gaaatatccc ttcttgagaa
     3841 aatgatttaa aaaatctggt tccctcattt tagaacctca taaacatcct tggctatctt
     3901 agaaatcgta tcataatcag aattcttagt gaaaatagaa agaataaatg gagaatctgc
     3961 atagacaaca cccgtatcat gcttaaattc atccgcatct ccaattttat gagctacttt
     4021 aacagaaaca cctttggcaa ttcgctgact atcaaaatct gttttagtca aagactctag
     4081 cacaaatcca ttttgattat aaatagcttc cataaacttc ccggccatct tagaagaaat
     4141 caatttttct tttggatccc aatcatctcc cataatggca gacatcttgg atttgaatgt
     4201 ggcatcagat tggtttgaaa tgtaatatcc caatagatta tgagctacat tatcagattc
     4261 ttttgatact ttcgtaatta aatcctttaa agaatattct ttattatctt cttttttagg
     4321 aagactacca cttccctctg gtttataaga acctggaaaa tcattgactg cagatacgta
     4381 ttttacagtc gtatctaact gataaagacc ctcatttatt ttttcttgcg tataatagag
     4441 ataagagagt ttcaaaacgc tggctgcata catcttttca tcttgattga taccagcttc
     4501 ttttccagta gtcagttgct taacataaat agagaaagaa tctttctgat atttttcaga
     4561 taacatttct tgaactttac tcatccgatt atcttcttca gaagttgatt ctttagctac
     4621 ccatccagcc tgatcaatat gtagaaattc tcttccttct acaaacatgg tcttgtcgat
     4681 tgatacttgc gaataagctg atgaggatga tttcacttct tttaaatcat aaggactatt
     4741 gtacagttta aagtcagatt ctaaccatgc tttttttatt gttggagtta cctctgattg
     4801 atcatataaa aatcgtttgt ccgcagctat aaattgatga tttgatagct taaatactgg
     4861 aattccttgt ttatttaagc gccactcggt tatttgaaaa cttgttttgg gagtcaattt
     4921 cccagattcc actactaaat cttcattcgc ataaacagga acctctccat aaaccatggg
     4981 agaacttaat ttttctctaa aataaatacc aaagtcagat tgtgaaaggt aataaatttc
     5041 tttcgaagta tagacgactt ctttttctgt gctaacgact tttgaaatgg tcaaaaaact
     5101 tggtagcaac aaaataatta agaatttacg cattcttcct ttccttttct tcttgtaaac
     5161 gcaataattg atttttatca gccaattctt ccagcttttc atctgacaaa ctcaaaaatt
     5221 gctctattac gtctaataaa ttttccattt tatcaccttt gaagcaagtc aggaatcgta
     5281 taaacttttt ccctcgactt agaataatag tacttcgctc gtgtatattt ggcagcataa
     5341 tcttcatctt tcaacttggt agcaaatgct gtctccttat ccttttcatc actcaaagtt
     5401 tgatactgag tttgcaagtc tgctaattgc tgacgtcttt ggagtaattg ctgataactc
     5461 tgcgctaaat taaaagttgg caagataaat aatagcataa tcaaaatcaa tacccctccc
     5521 ataaaacgat tccgtttttg tcgttctttc atcaggtagc gacgacgttg gtattcattt
     5581 tgaataaaag aattattcaa ttgtacaata tttttagaca ttttcttcta cccgtgtttc
     5641 actgataatt tcatacattc ctgctgcatc ttcttttttt gtactatctt tcatctctag
     5701 tacttttaca agcagcaact tattgccaaa gcgaatttca acttggtcat taactttcaa
     5761 gtccgttgaa cttttggcca agattccatt aaccttgatt ctacctttat ctgctacttc
     5821 ctttgcgact gtacgacgct tgataattcg cgatactttt aaatatttat ctaatctcat
     5881 ccttattacc tcaatttatt attgtaccat ttttatctat ttttatctat tttatagaag
     5941 aaaaatatca aatggaattt tcttccttaa actcttttat ctctaataaa ctttctccaa
     6001 aaatcagcag accttctaaa atttcataat ctttcttatt ttggacatca aatacaagct
     6061 ccattaatcc cttattctca gcgatgcctg cttttaagtt cgttacggat aaagctttaa
     6121 aataatcttg agctaaaaac aatcgttgag tgactttttc aaattgaatt gtaattttat
     6181 tatcttttct ttccacacgt tgaacaaaga ccttgtccaa gtatgattta accaaaccaa
     6241 tctctaaaag ataggctact acatctgggt attctccaaa acggtctatc aactcctctt
     6301 gtaactcttc ataattgaca cggttgtcaa tttgacgaat tttcttgtaa atttcaatct
     6361 tatgtcgttg atcagaaata taagtatcag gaagataggc atcaatttgc aaaatcaact
     6421 cagcattccc tttggttctt gtgttagcgt tagcattacc gtttcgttta gcaatagctt
     6481 cctctaataa ctgcgaatac aattcaaaac caacagaatc aatgaaacca gactgggatt
     6541 ttcctaaaag atttcctgct ccacgaatcg aaagatctcg cattgcaatc ttaaagccag
     6601 agcccaattc tgtaaatcct ttaatcgctt ctaatctctt ttcagagact tcactgattg
     6661 atttttctgg acgatacatg agataagcat aagcaatacg attactacga ccgactcttc
     6721 ctcttaactg atataaggtt gacaagccca tatggtccgc attttcaata aataaagtat
     6781 tagcatttgg aatgtccacc cctgtctcaa taatagtagt cgtcaccaag atatcgtatt
     6841 gtccctcaat aaagtctaat agagtatttt ccaactggac ttcactcatt cgaccatgaa
     6901 catatccaat cgaagcctcc ggaatcaact cctgtaattc tgaaaccttc tgaacaattg
     6961 tgtcaacttt gttgtaaaga taataaactt gacctccacg ctccatttca cgcaagacag
     7021 catcacgaat gacactatca ttcttttcca aaacataggt ctgaacagga tagcgattag
     7081 tcggcggagt ttcaataaca gataaatctc tgattcccag catagacata tggagggtac
     7141 gagggattgg cgtagcggtc aaggttagga catccacttg tttcttcagt tctttcaaag
     7201 tttccttatg cttgacacca aatcgctgtt cctcatcaat aatcatcaag cccaaatcag
     7261 caaacacaac atcttttgac aaaacacgat gtgttccaat caaaatatcg acttgaccgt
     7321 ttttcaattt ttcaagtgtt gcagtctgct cttttttact tctaaagcga ctcaacacat
     7381 caatattaac tgcaaaattt tggaatcgtt ccttaaaatt cgtatagtgc tgttgcgcta
     7441 aaaccgtcgt cggaactaga atgacaacct gtttgtgatc attgactgct ttaaaggctg
     7501 cacgcatagc aacttcagtc tttccaaaac caacatcccc aactaaaagt cgatccattg
     7561 gctgagaagc ctgcatatcc ctcttgattt cctcaatact acgaagttga tcatccgttt
     7621 caacataagg gaaagcatca tcaaaggcat cttgatcatc atcatcagct gagaaagcaa
     7681 aacccttcaa ctgactacgt tcagagtaga gtttgattaa atcatcagct atatcctcta
     7741 cctggttctt aaccttttgc ttggcctttt taaaatgacc gtcatttaat ttattgagtt
     7801 ttggagcttt accatcactt gaaatatatt tggacagtag atgaatctgt tccacgggga
     7861 tagaaatttg atcaccattt tggtattgga cactgacata atcgcgatga attcccttga
     7921 tttcaatggt ttcaattcct agatattgac caatcccatg gatatgatgg acaacatagt
     7981 cccctttttc aagttcattg taatctttta atctctctgc atttgaaaca tgttgtcttc
     8041 gaaaacgacg ctttaatttc ttttgaaaaa tctcatgttc agttatcaat aaaatctttt
     8101 catctacaaa atgaaaacca tgtctgagat taccctctat taaatttaca gattctttac
     8161 agatacttgt cttatctcta gaatccaatt taatctgata ttcctctaac atatcctcca
     8221 atgttttact tcccattgaa ttgctagact gcagaataat ggtgtaatcc atttttttat
     8281 atcgttcaat ttcttctttt agaaaagaaa actgattgaa aaattcctgt ataggatatt
     8341 gattgaattg ataaattttg tcaaatttga gatttcctaa acccttttga agattagaga
     8401 aaaaggtcac tggactttgt tttttataga tttgttcaat atcagaaaaa tactgcatat
     8461 cagaaaatgc tttactattc tgtaattctt ctgtaaagta ctgcgctaat tctctttcaa
     8521 agacttcata ctgattcatc aatttttgat aatcatcaaa gaatattgga gtatcttttt
     8581 caatataatc aaagacagtc catgtcttat catagcacaa agataaaaac ttccgagaat
     8641 ctgcatgact ttgtttttgg tgaaaacttg aaagaatttc ttctaggtat gatttcaaaa
     8701 taggtgataa agtttttgaa atttgttttt ctaaagctga ctgtcctcgt tgataatcct
     8761 tttctctcaa aagcatatca ctagctggaa agatagtgag ttctgtctta ttttctttcg
     8821 ataattgtgt ttctacttca aatgacctga taccatcaat ttcatcacca aaaaactcaa
     8881 ttcgacaagg ttctaactgg gatatttcaa aaatatctaa aatatctcct cgaagactaa
     8941 attcgccctg agtttgtact tgagtaactt tttgatagcc attttccttt aactgatgga
     9001 taaacgcgtg ttgagcatat tcttcaccaa ctgagatttt tacaatacta tctttgaatg
     9061 cattgggaga cggtaaaatc aatcgacttg ctgcgatatt acaaactaaa atccctttct
     9121 tagatgaatc agtcaaaaaa cgcaaggctt caacccgtga aataattttt tcctgtgaag
     9181 acatcaaaaa ctccaccata ggagcatcat ctaccaaaaa tggatagacg agttcctcac
     9241 ccaagataga aataagatca ctaacaagtc cttctgcttc tccataagtt gacgtcaata
     9301 acacaatcct atcttctttt tctaaactgc ttgcaattgc aagagcctta gtagatgttg
     9361 ataaaccaag tattagttgt cttttcttat ctgttaaatt ttgatgccat tttttaatct
     9421 gatcattttc tgagaataaa tctaataagg tcaccattta tccgttatac ctctgcattg
     9481 ttttctcaaa atttttctct tgtaaatagt attttacaga atcgtcaact ttgtcaacag
     9541 actgtaaaat accgatataa tcatccctgt caaacttact caaaacatga tgaacaactg
     9601 acataccatt tttaggtctt ccaattccaa tcttaacacg gttaaagacc tgagttccta
     9661 tatgttgaat aatagacttg ataccattat gaccacctgc tgagcctttt gctcttaaac
     9721 gaattttccc aacttccatg tcaagatcat cgtaaatgat aagtaaatcg tcaatatcca
     9781 aaccatagta agttaataaa gcatgaactg cttttccact ttcattcata aaggtcgttg
     9841 gtttaaccag ataaattttt tctccattta ggaaaaagga tgctaggtca gcttgaaata
     9901 tcttatcgtg tgtaaaagtg acattctgtt tcttcgctag ttgatcaatc aacataaaac
     9961 caacattgtg ttttgtttca aaatatttat cccctggatt tcccaaacct acaagtaatt
    10021 tggtcattta tttttccttt caaaagccaa aagggttgga atttttttcc aacctaattg
    10081 acaccattta ttaattttta gacattaaag cggaattcca tgatgtcgcc atcttgaacg
    10141 atatattctt ttccttcttc acgcaagcgt ccagcttctt ttacggcctt ttcagatccg
    10201 tatttcacta gatcttcata tgacatggtt actgcacgaa taaagccttt ttcaaagtct
    10261 gagtggataa taccagctgc ttgaggagcc ttcataccac gtttgaaagt ccaagcgcga
    10321 acttcttttt caccagctgt gaagtaagtt cccaatccaa gcaagtggta agctgcacgc
    10381 gtcaacttat ctacacctga ttctgtcaaa ccaatggctt caagaaactc ttttttatct
    10441 tcatcatcca attcagaaat ttcttcctca gcacgcgcag aaataacgac tacttcagca
    10501 ttttctgtcg ctgcaaattc acgaatttgt ttgacatagt cgatagagtc aggttctgaa
    10561 accacatcct cgtccacatt agctacataa agaactggtt tagtcgtcaa aaggaaaaga
    10621 cctttgacaa ccttttgttc ctcatctgta aattcaatgg tacgagctga tttcccgtct
    10681 tctaggactg gtttaatctt ttgaagaaca ttgaattctg ctactgattc tttatctttt
    10741 tgcgtacgtg ccatcttttc tacacgcgca tatcgtttgt tcactgattc taagtcagca
    10801 agaattaatt ccagattaat tgtatcaata tctgcaagtg gatctacaaa ggcgtcttca
    10861 cgtccttgct cgcgcatcac attttcatca tcaaaagcac gaactacgtg aacaatcgca
    10921 tctacttcac gaatattggc caagaattta ttccctagcc cttctccttt tgaagctcct
    10981 tttacaatcc ctgcaatatc cgtaaattca aatgttgtgg gaactgtctt tttaggagtt
    11041 atcatttcag ttagtttttg taggcgttca tctggaactt ccaccattcc aacatttgga
    11101 tcaatcgtcg caaatgggta gtttgctgcc tctgctcctg cttttgtaat tgcattaaat
    11161 agtgttgatt taccaacgtt tggcaaacca acgatacctg ctgtcaaagc catatttctc
    11221 attctccgtt ttcatttcaa tctctaatat tataacacaa aaagggataa agtgctaacc
    11281 ctctaactaa gggttctcag tcaataattt tattcatttt tcgctcaaaa tcatatcgcc
    11341 ccatcatgac aacatgctca caattactgc attttatttt gatatctgct cctacacgtg
    11401 taatttccca acgattagcc tttttaccag tcgacttgat tgtacaagcg tgtgattttt
    11461 tcatctcaac aaaatttcca acttgataca tactactctc cttttctttt tcgattatat
    11521 cataaaagag gcgagccagg ctcaacctct ttcacttaat ttgtacgaac tggtgtaatg
    11581 agctgcatga agtcttcgtc agtatctgct ggcacaagag taaatggacg aacagctgag
    11641 ataaagctaa tggtcacctt ttcgctattt aaagctttaa gagaatcaat caagtaagtt
    11701 gggttgaaac taatggtcaa atcttcacca gtaacctgat cagtatcgat ttcttcgttt
    11761 actttaccaa cttctggaga gtgaacatgg gcgctaacaa ccccatcctt aatttcaagt
    11821 ttcacagtac cattttgagt cgcacttgat aaaagacggg cacgctccat tgactggcgt
    11881 aagtttacca cattaaaagt aatagtagtg ttaaagtctg ttggaatcaa gcgatctgta
    11941 tcaggatagt ttccttctag gagacgagta tagaagctaa tattttcgct tctaaagagg
    12001 atttggttat tggcaaagaa aatctctaca gtttcgatat catctgtaaa taccgctgaa
    12061 aattcgcgta gagaacggct aggaattacg acatcaaaat catcactatt tttttcaaga
    12121 gtcaattttt tctggcttag gcgatgagag tctgttgcaa ctgtttttaa ctctttgtgt
    12181 tgactcaata cgaagtggac acctgttaaa atcggacgac tctcttgtgt acttgcagca
    12241 aaggctgttt cattaataat tttcttgagt aattttgttt caagtattaa aggagtgctt
    12301 gctgaaattt cttggattcg tggatattgt tcgctatctt ttccttttag ggtaatttct
    12361 gatttgccac tggttaaaac aatttgattt tgttcaattt ctttaaaatc aagagttaca
    12421 tcaggtaaac tagatactac attgataaag aaagaagctt caagaaggat cgaacctaaa
    12481 gaagtaatta acaaaccagc atcttcattt ttttgagaaa taaaattttc aattgaaatt
    12541 tgaccatttg aaccaattaa agtaatacct tcattggtca cgtcaatttt tactgttgat
    12601 aaaataggaa tggcattttt agaactaata gctctcttag tagtatttaa tgcttgtaga
    12661 aataaatttt tattaattga aaaatgaatc atggattctc ctttatttat ttttagtatt
    12721 agaaagataa tagtaataat agagtctgtg aaatctgtgg aaaacagcct ttaaacaagt
    12781 catatcaagt tttttagctt gtttaaaaaa tgtggataaa aaagataaaa aactaaaagt
    12841 tatccacaaa ttatttgatt ttctttttga ttgattcaat ttctaaacgt aaattatcgt
    12901 cttgatcaat caaagatttt attttggcat gggcatgaat gactgtggta tgatcttttc
    12961 ccccaaattc cttcccaatt tttggaagac tattatctgt tagttctcta gataaataca
    13021 tggctacttg acgggccaaa acaatatttt gaaggcgtct acttcccttc atttctttga
    13081 tactaacacc ataaaagtta ccaacttcag tttggatttt atcaattggg atgacgagca
    13141 tttggctaac atcttgtttg cgggctctaa tggcttctgc agcaatatca atagtgatat
    13201 ccttgatttt ttttactctg gcaattaaag tgatgtcgtt gatggctccc tcaagatctc
    13261 gaacatttga atcaaattgc ccagctaggt attctagagt atcactttgg aaattgtagc
    13321 ctaaatgttc cgttttactt tgtaaaatgg caatacgtgt ttcaaagtca gggggtgtga
    13381 tagtttgtgt caatccccaa ctaaaacgcg tgacaagcct ctcctcgagc ccttctagat
    13441 gttttggact acgatcactc gttaggacaa tctgtttttg cttgtcatga agggcgttaa
    13501 aggtattgaa aaattcttcc tgagttgcga ctttttttcc gctgagtgac tggatatcat
    13561 cgattaacaa aagatcaaga ctacgatagg tctttttaaa cttttccatt tccccaagtc
    13621 ttaggtgatc aagaaagtca ttaataaagc tttcggcagg gatatattta acacgcgcat
    13681 taggaatatt ttttagaatt tcatttccaa tagcgtttaa taagtgagtc ttaccaaggc
    13741 ctggtcctcc atagataaaa agagggttat aggtcagagc caaatcttca gagacagcta
    13801 aagcggctga cacagcccaa acatttccat ccccttgaat aaagttatca aaggtatact
    13861 tttcttttaa tcccgtatct gaataaggaa tagatactaa ctttggacta tagttataaa
    13921 gagttaaatt tgtagcttct tcaacttgtg agctagtcgt atcttgaggt ttggtgaaaa
    13981 tatagtgggg agttatttca gcgtcataaa tttcaaaacc agctactaca ataatatctt
    14041 ttagttgttt ttcccagacc atttccattt cagagcgagg tagaaatata gtggcaacat
    14101 tttcctctac cttgatgagt tcagcttgaa tagcatagaa atcatacatg gatcgagtca
    14161 gtctttcttg tgcaaattct aatatacgat tccaaaattg tttttctttc aatcctttct
    14221 cctcctttac tatttaaaag tttaatacta gagatatttt accatagata gcaagaattt
    14281 tccacaagct gtgaaaaata atccacaatg ttaccaaact ttatccacag gttggggata
    14341 aaagaagaaa ttattgattt attaagcttt ttattgaaaa acatagtgga taaccttgtg
    14401 agataaaaaa ataaaagaac agccttattt caggctgttg ataattctac tatattcttc
    14461 ttgatttgaa aaagaaataa tgatttttcc actgtctttt ttagatagtt taatttctac
    14521 atctaatccg agtagttttc ttaactgttt ttcttcattt tgtatgaaat gattagtttt
    14581 ttgctgtttc ttttgttttt tctctgtcag aagagcttct aatttcctta cagaaatatc
    14641 ttcttctata atccgttgaa agaaatagtc ttgttgttcc ttatttaacc caactaggga
    14701 acgcgcatgg gcttgtgata gtttgccatt ttctacttct gaaagaatct gttctggcaa
    14761 ggaaagtaaa cgaatggagt tgctgatata tggacgagac ttgcccatct tatctgcaat
    14821 ttcagcatgg gtgaatcctt tctctacgag agattcatag gcgcgtgctt cttctattgg
    14881 gtttaaattt tctctctgta aattttcaat aatggactgg accatcatct cttggtctga
    14941 aatctgttta acaacagctg ggatagaccg tagaccagct aaaagtgaag cccgatagcg
    15001 tctctctcct gcaaggattt cataaccaat aacaggagat tgacgaacaa taatcggttg
    15061 aatgacccca ttttctttga tagactgtgc tagttcatct agtttttctc tatcaaattc
    15121 ttttcggggt tgatagggat ttttttgtat atctgtgata gaaatcattt caaatttttc
    15181 catgattcta cactaacaca tcttttctct tatgtaaagc tttctttaca tagatgtcaa
    15241 ttaagattct aaatcacctg aactcttgtt aagtttgata gaggtagttt cttctttccc
    15301 gttacgatag taggttatct taatggtgtc tccgatagaa tggttgtaaa gagcactttg
    15361 taagtctgtt gatgaagcaa tctctttgtc atctactttt gtaattacat cgtatttttc
    15421 aaggtgacca ttggcaggca tattactttg taccgaacga acaactacac cagatgtaac
    15481 attacttgga atattgagtc ttctgatgtc gcttgtactc acattagata aattaaccat
    15541 ctggattccc aaagctggac gcgtcacttt tccgtttttt tctaactgtt caataatatt
    15601 gatagcatca tttgcaggaa ttgcgaaacc aagaccttct acagatgttc ctccatttgt
    15661 agcaatttta cttgaggtaa ttccgataac ctgcccttga atattgatca gtgggccgcc
    15721 agagttacct gggttaatag cagtatcagt ttggatggct tttgtagaaa tagcttgtcc
    15781 atcttccgat tttaaggata catttctatt gagactggat acgatacctt gagtgacagt
    15841 atttgcatat tcagaaccta acgggctacc gatggcaata gcagtttctc ctacagttaa
    15901 cttactagaa tcaccaaact cagctactgt tgtcactttt tctgaagaga tttttacgac
    15961 agcaatatca gagaaagtgt cagctccgac aatttctcca ggtactttag tcccatctga
    16021 caatcgaata tctactttgc tggcgccatt tataacgtga ttgttggtga cgatgtaagc
    16081 ttctttatca ttctttttat aaataactcc agatccttca ctagagattc gctgagaatc
    16141 tgtgtcagta tcatcattgc caaatacgct attttgtctg tttgccgaat aagtaataac
    16201 agaaacaaca gcatctttta ctttgttaac agcctgtgtt gttgaatttt cgttcttata
    16261 ggcagtttgt gtaatagtac tattgttgtt agagttgttt acactacttt tttgagttag
    16321 ttgagttatt gaaaaactac ccaaggctcc actaaaaaag ctaatgacga taacgactaa
    16381 taattgaaac cattttttgt aaaatgtttt tagatgtttc atatttgcct ccatatgttt
    16441 gaattactga aagtataaac tgactagctt aattataact taaacacaaa agttttacac
    16501 aaactgtgga taactctttt gaaactgtga ttttcttaat tgaaatctat tttttatttt
    16561 gtgaataaga tgtgaaaaaa tagagaatat gttagaatag agtcatgaaa attaaagttg
    16621 taacagttgg gaaactgaaa gaaaagtatt taaaagatgg tatcgcagag tattcaaaac
    16681 gaatttctag atttgctaag tttgaaatga ttgagttatc agatgaaaaa acaccagata
    16741 aggccagtga atcagaaaat caaaagattt tagaaataga aggtcagaga attttatcaa
    16801 aaattgctga ccgtgatttc gttattgtgt tagccattga agggaaaact ttcttctcag
    16861 aagaatttag taagcagtta gaagaaactt ctataaaagg attttctact cttactttta
    16921 ttattggggg aagtttagga ttgtcatcat ctgtaaaaaa tagagccaat ctttctgtca
    16981 gttttggtcg cctaacctta ccccatcagt taatgagact agttcttgtt gaacaaatct
    17041 atcgcgcttt tacgattcag cagggattcc cctaccataa atagaaaatt gacttttaat
    17101 tgaatttttg gtagaataat tgtattaggt ctcatagctc agctggatag agcattcgcc
    17161 ttctaagcga acggtcgcag gttcgaatcc tgctgggatc aatataatag caaagctggg
    17221 aattttcccg gcttttttct taaaaaagta cactttggga gaaaaaaatg acagttgaga
    17281 gaattttatc taaaacgaaa ttccattttg tataatggtt tttgtaagtt agcttacaag
    17341 aaaaaacatt ttaggagatt ttattatgaa aaacacagtt aaattggaac agtttgtagc
    17401 tttgaaggaa aaagacttac aaaagattaa aggtggggag atgaggttgt caaaattctt
    17461 ccgtgatttt attttacaaa gaaaaaagta atgaaataag gggaaagagt aatggattta
    17521 tttggatttg ggacggttat tgttcatttt ttaattatta gtcacagtta ccattttatt
    17581 tgtaaaggtc aaataaatag aaaagaatta ttcgtttttg gtgcttatac attactaaca
    17641 gaaatagtat ttgattttcc cttatatatt ctatatttag atgggttagg gattgaaaga
    17701 tttttatttc ctttgggatt atattcctat tttcgatgga tgaaacagta tgagagggat
    17761 agaggactat tcctaagttt actactatct cttttatatg agagcactca taactttctg
    17821 tccgtaactt tctcctctat aacaggagat aattttgttt tacaatatca tttcccattc
    17881 tttttcgttg tgacggtgtt aacctatttt gttacattaa aaatcattta ctatttccat
    17941 ttggaactag cctattttga cgaggactac ctttatcctt tcttgaaaaa agtatttttt
    18001 gctttactat tgctacatat tgtatctttc gtttcagata tggtaagtac gattaaacat
    18061 ttgaatagtt ttggaagtat tttgtcatct attgtcttta tctctctcct tttgaccttc
    18121 tttgcaatga attctcataa agttcaaatg gagaaagaga ttgctttgaa gcagaagaaa
    18181 tttgaacaga aacatttaca gaattacaca gatgaaattg ttggtctgta taatgaaatc
    18241 cgtggttttc gacatgatta tgctggaatg cttgtcagca tgcagatggc aattgacagt
    18301 ggtaatttac aggaaattga cagaatttac aatgaagttt tagtcaaagc aaatcataaa
    18361 ttgcgttcag ataagtacac ttactttgat ttgaacaaca tagaagactc agctttacga
    18421 agtttggttg ctcagtcaat tgtctatgct cgaaataatg gtgtagagtt tacactggaa
    18481 gtaaaagata cgattaccaa gcttccaatt gaactattgg atttggttcg tatcatgagc
    18541 gttttattga ataatgctgt cgaaggatcg gctgatagct ataaaaagca gatggaagta
    18601 gcagttatta agatggaaac tgaaacagtt attgtgattc agaattcatg taaaatgacg
    18661 atgactcctt caggagatct atttgcctta ggattctcca ctaagggaag aaatcgcgga
    18721 gtcggattaa ataatgtgaa agaactacta gataagtaca acaatattat tttagaaaca
    18781 gagatggaag gcagtacatt tagacaaatc attagattta agagggaatt tgaatgaaag
    18841 ttttaatttt agaagatgtt attgaacatc aagtgagact agagagaata ttggatgaaa
    18901 tttcgaaaga atcgaatatt ccaatatcat acaagacaac gggaaaagtc cgtgaatttg
    18961 aagaatacat tgaaaatgat gaagtaaatc agctttattt cctagatatc gatattcatg
    19021 gaattgagaa aaagggattt gaagtggctc agctcattcg tcattacaat ccttacgcta
    19081 ttatcgtctt tatcactagt cgatcagagt ttgcgactct aacctataaa taccaggtat
    19141 cagccctaga ttttgttgat aaggatatca atgatgagat gtttaagaag agaattgagc
    19201 aaaatatctt ctacacgaag agtatgttac ttgaaaatga agatgttgta gattatttcg
    19261 actacaatta caagggaaat gatttaaaaa ttccttacca tgatattttg tatattgaaa
    19321 caacaggggt ctctcataaa ttgcgcatta ttggtaagaa ttttgcaaaa gagttttatg
    19381 gtaccatgac agatattcag gaaaaggaca aacatactca gcgattttat tctcctcaca
    19441 agtcattttt ggtaaatata ggcaatatca gagaaattga tcgaaaaaac ttagaaattg
    19501 ttttctatga agaccatcgt tgtcctattt caagattaaa aattagaaaa ttaaaagata
    19561 ttttagagaa aaaatctcaa aagtgattga caattagcaa gaaattgata taatggttat
    19621 atctgctaca gatagaaggt ccgttggtca aggggttaag acaccgcctt ttcacggcgg
    19681 taacacgggt tcgaatcccg tacggactat tttatccgcc atagctcagt tggtagtagc
    19741 gcatgactgt taatcatgat gtcgtaggtt cgagtcctac tggcggagta tagataaaag
    19801 ggaacacagc tgtgttcctc tttttgtatc aatttgtatc accaagcatt ttcataagga
    19861 agtctgttat ttcttgagaa ctttcttttt ttccatgtgc aatccaagtt tggcagacac
    19921 caaaaagtgc atgagttaga tagatgctac tatattctaa ttcggtggta tttagattca
    19981 gttgcataaa tcgcttttgt aaatctgtac taagcatgat atgaagttta tttcgtaaga
    20041 aattttggat ttctttagtc ccattttcag aaagaagggc agccagaagt ggttctgact
    20101 ctagatattc aaaaacttct aaaatagcgt ctcttttgtg atgagcatgt ttttgaaaaa
    20161 tatattcaaa tgtatggaat agcttgcttt gatagtgctc aatcatatca tacttatcct
    20221 tatagtgagt atagaagctg gaacgactaa ttccggcttt ttctactaat ttgacagtag
    20281 aaattttatc aaatggctgt tccatcagta attgtaccat agcattttca atagttcgct
    20341 ttgtttttaa gcgtttgtta ctttcttgca tatttcctcc ttgtaaacaa attagactat
    20401 atgtctaaaa atagattttt tatcttgtaa tttagatttt ttaatgtata atctattata
    20461 tcaaaatttt agacaatatg tttaaaaaag gagaaactaa gtttaaagaa tggaaagcaa
    20521 tttaaaaaaa accaaccttt attattgtca tgatcgggat ttctcttatt ccagatctgt
    20581 acaatatcat atttttgtca tcaatgtggg atccatatgg gcaattgtct gacttacctg
    20641 tggcagttgt aaataatgat aaagaggctt cctataatgg taatactatg gcaataggaa
    20701 aagacatggt gtccaattta aaagaaaata aaaccttgga ttttcatttt gtagatgaag
    20761 aggaaggaaa gaagggattg gaagatggcg attactatat ggtagtgact ttaccaagtg
    20821 atttatctga aaaaacaact acattatcca atattcaatc gacagcagct tatcaatcat
    20881 tgacaagtga gcaacaaact gagataagtg attctgtatc tcaaaattca actgatagta
    20941 ttcaatcggc tcagtcaatt gtagctttag tacaagattt acagggaagt ttagaaaact
    21001 tacaaaatca atcttctaat ctttcgactt taaaaaatca atctaatcaa gtatcaccta
    21061 ttacttctac ttctttgata ggattgtcaa gtggattaac agagatacaa ggagatgtta
    21121 ctagcaaatt agttcctgcc agtcagtcga ttgcatcagg tgtaaacgca tatactacag
    21181 gtgttgataa agtttctcag ggcgcaagtc aactaagtga aaaaaatgcc accttgacag
    21241 gtagtttgga taaactagtt tcaggctcaa acaccttgac acaaaaatct tctagattga
    21301 cagcaggagt tggttaatta caatcaggat ctgggcaatt agcagacaaa tccagtcagt
    21361 tactttcagg tgcttctcca ttagagaata gagctaataa attggcagat ggatctggga
    21421 aactagcaga aggtggaaca aagttaactt ctggattgga agatttacag acaggacttg
    21481 cttctttagg acaaggacta ggtaatgcta gtgatcaact caaatcagta tcaacagaat
    21541 ctaaaaatgc agagattttg tcaaatccac tcaatctttc aaaaacagac aatgatcaag
    21601 ttcctgtaaa tggaatcgca atagctcctt atatgatatc agttgctctt tttgttgcag
    21661 caatatcaac aaatatgata tttgcgaaat tgccttcagg acgtcatcca gagagccgtt
    21721 gggcttggtt gaaatctcga gctgaaataa atggtattat agctgttttg gcaggaattt
    21781 tggtatatgg aggagttcag cttattggtt taactgctaa tcatgagatg agaatattta
    21841 ttctcatcat cctaacaagt ttagtattca tgtctatggt gaccacttta acaacgtgga
    21901 atagccgtat aggagctttt ttcttactta ttttgctttt actacagtta gcatcaagtg
    21961 caggtactta tccacttgct ttgacaaatg atttctttag atctattaat ccctggttac
    22021 caatgagcta ttcagtttcg ggattacgac aaacaatctc tatcaacaag tcattttcct
    22081 agctgtcata ctagctctat ttactggttt aggtatgcta gcctatcaac ataagaaaat
    22141 ggaagaagat taaaaaaatc gaccgattaa tgaactgcac cccaaaagtt agacagaaaa
    22201 aatctaactt ttgaggt
//
LOCUS       DAVBGY010000031        21993 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVBGY010000031 DAVBGY010000000
VERSION     DAVBGY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21993
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.031"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(<1..217)
                     /gene="tnpB"
                     /locus_tag="VQ620_001208"
     CDS             complement(<1..217)
                     /gene="tnpB"
                     /locus_tag="VQ620_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET2763830.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKYRFKALYWDGQGFWLLYKRF"
     gene            complement(198..383)
                     /locus_tag="VQ620_001209"
     CDS             complement(198..383)
                     /locus_tag="VQ620_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763831.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(687..2249)
                     /gene="guaA"
                     /locus_tag="VQ620_001210"
     CDS             complement(687..2249)
                     /gene="guaA"
                     /locus_tag="VQ620_001210"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET2763832.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            2391..3089
                     /locus_tag="VQ620_001211"
     CDS             2391..3089
                     /locus_tag="VQ620_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2763833.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(3132..4028)
                     /locus_tag="VQ620_001212"
     CDS             complement(3132..4028)
                     /locus_tag="VQ620_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET2763834.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLLIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(4037..4972)
                     /locus_tag="VQ620_001213"
     CDS             complement(4037..4972)
                     /locus_tag="VQ620_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HET2763835.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4969..5694)
                     /locus_tag="VQ620_001214"
     CDS             complement(4969..5694)
                     /locus_tag="VQ620_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HET2763836.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(5778..6413)
                     /locus_tag="VQ620_001215"
     CDS             complement(5778..6413)
                     /locus_tag="VQ620_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HET2763837.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(6415..7242)
                     /locus_tag="VQ620_001216"
     CDS             complement(6415..7242)
                     /locus_tag="VQ620_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2763838.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(7264..7600)
                     /locus_tag="VQ620_001217"
                     /pseudo
     CDS             complement(7264..7600)
                     /locus_tag="VQ620_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
     gene            complement(7601..8317)
                     /locus_tag="VQ620_001218"
     CDS             complement(7601..8317)
                     /locus_tag="VQ620_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET2763839.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISMADII
                     EKAGITPIGVYTHEPRDQAPLVIKEVNAIKVALLAYSYGFNGIEQYIFQEDYNRYLSD
                     LNEDKIKAEVERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSETVEKDGDKKLIIY"
     gene            8840..9451
                     /locus_tag="VQ620_001219"
     CDS             8840..9451
                     /locus_tag="VQ620_001219"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET2763840.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEGIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9496..10224
                     /gene="rlmB"
                     /locus_tag="VQ620_001220"
     CDS             9496..10224
                     /gene="rlmB"
                     /locus_tag="VQ620_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET2763841.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFLNRL"
     gene            complement(10263..11174)
                     /gene="trxB"
                     /locus_tag="VQ620_001221"
     CDS             complement(10263..11174)
                     /gene="trxB"
                     /locus_tag="VQ620_001221"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET2763842.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVKEIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGSIAGQEAYKFITEHS"
     gene            complement(11240..11464)
                     /locus_tag="VQ620_001222"
     CDS             complement(11240..11464)
                     /locus_tag="VQ620_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HET2763843.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(11542..12285)
                     /locus_tag="VQ620_001223"
     CDS             complement(11542..12285)
                     /locus_tag="VQ620_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2763844.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAMSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHHPKEERTKEFFASYKRTYI"
     gene            complement(12285..13085)
                     /locus_tag="VQ620_001224"
     CDS             complement(12285..13085)
                     /locus_tag="VQ620_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2763845.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(13243..13599)
                     /locus_tag="VQ620_001225"
     CDS             complement(13243..13599)
                     /locus_tag="VQ620_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET2763846.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13612..14124)
                     /locus_tag="VQ620_001226"
     CDS             complement(13612..14124)
                     /locus_tag="VQ620_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET2763847.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(14124..14618)
                     /locus_tag="VQ620_001227"
     CDS             complement(14124..14618)
                     /locus_tag="VQ620_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2763848.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            14753..15199
                     /locus_tag="VQ620_001228"
     CDS             14753..15199
                     /locus_tag="VQ620_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HET2763849.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            15196..15843
                     /locus_tag="VQ620_001229"
     CDS             15196..15843
                     /locus_tag="VQ620_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET2763850.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(15864..16445)
                     /gene="pdxT"
                     /locus_tag="VQ620_001230"
     CDS             complement(15864..16445)
                     /gene="pdxT"
                     /locus_tag="VQ620_001230"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET2763851.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(16446..17321)
                     /gene="pdxS"
                     /locus_tag="VQ620_001231"
     CDS             complement(16446..17321)
                     /gene="pdxS"
                     /locus_tag="VQ620_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET2763852.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(17473..18852)
                     /locus_tag="VQ620_001232"
     CDS             complement(17473..18852)
                     /locus_tag="VQ620_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2763853.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKDKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            19147..20070
                     /locus_tag="VQ620_001233"
     CDS             19147..20070
                     /locus_tag="VQ620_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET2763854.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGISPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            20130..20735
                     /locus_tag="VQ620_001234"
     CDS             20130..20735
                     /locus_tag="VQ620_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2763855.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKTIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            20753..>21993
                     /locus_tag="VQ620_001235"
     CDS             20753..>21993
                     /locus_tag="VQ620_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2763856.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRNFKFKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGAS"
BASE COUNT         6579 a         4955 c         3984 g         6475 t
ORIGIN      
        1 caaagcgttt atatagtagc caaaatcctt gaccatccca gtaaagggct ttaaagcggt
       61 atttacgtcc accacaaaag agaaaaactt gacctgagaa aggatctaat tcaaagtggg
      121 ttttaaccag ataagccagt gaatcaatgc cttgcctcat atccgttttc ccacatacga
      181 gatagacctg ccctaggcta gatagatgaa ttgtcataga gcaacacctt atccaaaagc
      241 tgttctacca tttcgagatt gagagattga aaaaaactta cttcaagctt gccaatacga
      301 attttaagca gaatatcatt tctcttttta gaatcaaaag gacgagattg tggaatctct
      361 acaggaacaa taggttgtgg cattaaaata tcctccaata gttttacttt actaatagta
      421 tacaggagga actccatgaa tgatagatac cttgttatga cgcgattact atattagaaa
      481 gttggctctc taattaaatt aattctcatt atatatatca aaagaccgca gcttgaaaac
      541 tacggtctaa ttttttgtcc aaatgtaaac cacttggaac tattattcaa tttattcttt
      601 ttagtaaatg ttcccgtttt aatcccagtt acagttttta atcctctgta accgttgatt
      661 ttacaaagct tttaattttt ttgcgattat tcccactcaa ctgttgcagg tggtttactt
      721 gtaatatcgt agacgatacg gttaacatga tccacttcat ttacgatacg tactgagatt
      781 ttttgaagta cttcccatgg aattttggca aaatcagcag tcataccatc gatagaagtg
      841 atagcacgga ttgcaatcgt gtagtcatat gtacgaccgt cacccataac accgactgaa
      901 cgaacgcctg tgttaacagt gaagtattgc caaatatcgc ggtcaagtcc agctttagcg
      961 atttcttcac gaagaatagc gtctgattca cgaacggttt caagtttctc ttcagtgatt
     1021 tcacccatga cacggatagc aagtcctggt cctgggaatg gttggcgcca tacgatatgg
     1081 tctggcatac caagctctgt accaagagca cgaacttcat ccttgtaaag agtattgagt
     1141 ggttcaatca attcaaactg catatcttct ggaagaccac ccacgttgtg gtgtgacttg
     1201 atagtttgag ctgtatccgt accagactcg ataacatctg tatataaagt accttgagca
     1261 aggaatttca catctttgag cttgcttgct tcgtcatcga atacatagac aaactcgtta
     1321 ccgatgattt tacgtttttg ttcaggatca gaaacgccag caagtttgtc aaggaaacgt
     1381 ttagcagcgt ctgctttgac gatattcaaa ccaaacttac caccgagcat gtccataact
     1441 tgatcagctt cgcctttacg aagaagaccg tggtctacga agatacagat caattgatcg
     1501 ccaatcgctt tttggagaag aaccccaacg acagatgagt caacaccacc tgatagacca
     1561 agaaggacac gtttatcacc gacggtttca cgaatttttt tgatctgcat gtcaatgaaa
     1621 ttatccattg accagtcacc tttagcctta caaatgttaa gggcaaagtt acgaaggata
     1681 tcatttccgt atacagaatg acgaacttct gggtggaatt ggataccgta aatgtgttta
     1741 tctgggtttt cgatggctgc gtatgggcag tcagctgatg tacctgtacg aacaaagtca
     1801 gcaggaatct cagtaaccgc atcaccatgg ctcatcaaaa cagtctgttc atcaggtgtt
     1861 gattcaaaaa gcgctgatgg tgtgtgagtt agggttgatt gaccgtattc acgatttcca
     1921 gcatcacctg caggaacaac ttttcctcca agtttatggg tcaataactg cataccataa
     1981 cagattccca aaattggaat tccgagttcg aagatttctg ggtcaatatc aaatgaacca
     2041 tcttcatata cagaatttgg accacctgat agaataattc ctacaggatt gacttcacga
     2101 acttcagcag ctgaaatttt atggcttttt agttctgaaa aaacaccaat ctcacggata
     2161 cggcgtgaaa tcagctggtt gtactggcta ccatagtcca atacgatgat tttttctaca
     2221 tcttgcaaat cagttgaaat gttgctcatc tttttccttt ctcaaaagaa atatcttttc
     2281 tcaatattct atcacaaata agggcgaatt accaactaaa caaggcgaag tttgactttt
     2341 tctagtttat tggggtacaa tagagaaaag atagaaatga agtgaaaaat atgctacctg
     2401 cttatatgaa aatccatgat cagataaaaa aggatattga cgagcaccgt tgggctattg
     2461 gtgagaggct tcccagtgaa agagatttag ctgagcagtt tgcggtcagt cgcatgaccc
     2521 tccgccaagc cgtatctcta ttagtcgaag aaggcgtctt agagcgccgt gtaggaagcg
     2581 gcacctttgt ttccagtact cgagtacaag aaaagatgcg agggacaacc agttttactg
     2641 aaattgtcaa atcccaaggt aaagttccct ctagccagct catttcctac aaaaaaacca
     2701 ttcccaatga gcaagaagtt gccaagctag gaatttttcc aacggacaat attatccgaa
     2761 tggagcgggt ccgctatgcc gaccaagttc ccctagttta tgaagttgct tctattcctg
     2821 aaaaattcat taaggacttt aaaaaagaag aaatcacaag tcatttcttc caaaccttgc
     2881 aaaaacatgg ctatcgtatc ggcaaatctc aacagaccat ctatgctcgc cttgctaaag
     2941 aaaagattgc ccactatttg gaagttgaaa aaggacatgc tattcttgga ttgacccagg
     3001 tttcctacct agaagatggt actgcttttg aatacgtaaa aagtcaatat gtaggcgaac
     3061 gctttgaatt ttatcttgaa aataattaga ttaccacgca aaaactctct gtcacggaca
     3121 aagagtcttt tttatttttc taagaataaa tctttcaaag aaatcaaagt tacagcaaac
     3181 aatatcattg ccataccaag aaaatcaatg ggataaaatt gttcattcat tattaagaag
     3241 gcaaagaaaa tcgccgatat tggctcaatt gaagccaaca agcttgactt gaccggtcct
     3301 atcagactgg ctcctttaag gaaagctgta taggcaaaga cagtcccgat aaggataatg
     3361 cctgcaaacg caaggagaaa atcaagacta gtcgggatat cggcctgtag aacccctgta
     3421 aaaggaaggg cgaccaaacc tgctatgacc attcccacac caatgagcaa gctgctcccc
     3481 cactttttaa tcaaggctat gggtaaaatg atatacagag cataagtcaa ggcagaaaag
     3541 agaccccaga acagaccagc aggtgtcatg gataactggt ccaactgccc atgtgttgcg
     3601 atcaggaagg ttcctccgat ggcgaatatg atggaaacta tctctcccag tgtcggtgcc
     3661 accctatcct tgatacagct ataaattaaa attccgacag gacaaacata ctgaagcacc
     3721 gtcgctgttc ccgcattggt ctcctgaata gcagacagat aggcgaattg gttgagaaaa
     3781 agaccaatca gagcaaaaat aagaagagac agcaaactct ttctatcctt taaaaagacc
     3841 agtattttat cctttgcagt agcataagcc aagagcatga gaattccacc agcgattaaa
     3901 agacgcaagt tggtcaagac cagagccgaa attccgtgtg ccattaggta ttggccactc
     3961 gttcctgaca acccccaagc aataccagcc acaactgtta gtaaagtccc ttttaaacta
     4021 tttgacatgt ctctccttac tcttctttgc gaatcaagtt catcacttga ttgcggtcta
     4081 atatccattg agctctctcg tccttttcat aggtacgatt cgataggaaa atagtggctt
     4141 cttgcttctg acgattccac atgataaagg tacctgtata gcccgtatgg tctagccaat
     4201 ctccttccaa attccatgct aaagaacgtt ccttgtcatc caaaggagaa aaattttgat
     4261 ttaagtctct tgcaaaatca tctgctaaat agtgttctaa aaagatttgt aaatccttta
     4321 tagtcgaaaa taaaccagca ctcccagcat gtctacccag gagacgagcc ttgggatcat
     4381 gcactatgcc tgcctctaca cctctaactg ttggaacagc aagctcaact ggcccaaact
     4441 tggtttccgt cattccccaa ggtttccaga cttgatcctt taaaatcaca tccaaatctt
     4501 gattaaaaat tctttccaaa ataaagccca acagcaaaaa atggacatcc gaataaagaa
     4561 aggctggctg acttcgtctg ttgagatgaa acatcgcttc ctttaattca ggggctgtta
     4621 aaagatcacg attaggaata aaaggatcaa ggtctgttgc atgagtcaag agctggcgaa
     4681 tagtgatgtc tggataatca ctctcaggta aaaaatctat taccagtcta tcaatatcta
     4741 attgacctat ttcccacaag aaggtacaaa ctgtgccaac cccaacaacc ttgctgacac
     4801 tagctagatc ataaactagt cctgcctcag tctgcaagcc atgctctggg tcactctggc
     4861 ctaaatagaa ctctgtccat tgattgtcct taaaatacgc aaaagaggct ccgggataaa
     4921 ttcctgcctc gatttgttct tctatttttt taataatctt ggtccacttc atacttcttc
     4981 aaaccacaat tcaacattat ttctatcttt accaaggaag aatttttcag acttaggaat
     5041 aaaatattca gtagactcaa acttctggcg aagacttgct atgtctaatt cattgaccaa
     5101 gaacttgagc atagataagt cccaggtaac cgtattgtcc acagtcaaat cctgcccctg
     5161 agctggaata aaatcaaggg atgccccaat ttcagatgat tccaagaaac tttcgatatc
     5221 agttgggaga tgtaattcca tagaaatctc aaatttactt aaagaaattg atgccaaatc
     5281 tgtttgaaat tcaggctttt ctcctacttc tactagactt gctctgtcat cttccgcatg
     5341 aatcaaaatc aaatcatctt ctggtgagaa aatttcaaaa gcgtagccat tttgaccttt
     5401 atataatcga tgaatcgaat ctgttttaga taagattcct tcaatttcta agggattttc
     5461 caccttgaca atcaatctag ctagtttttt tcttccctct accttacgag tacgcatact
     5521 gggagcttct tctaaaacca gcttttcaag acccgtttgg tcacctagtg acagaaaggc
     5581 cgattcttct aacaaggcct tcattccaag ggtttcaata taaaatgttt catttaattt
     5641 tctattatta gcttttaaag taggaataat ccgtacaatc tgatttacat tcataaattc
     5701 ctccgtcact ttttatttta aaggatttta gaatttttta caagatatta tgctcaaatt
     5761 taacattatt tagctattta gtaaagatag tctttcaagg attttgacaa agcctgataa
     5821 ccagcaatac tgaggtgcag tccatcagtt gtatattctt ttttgagttg gcctgcttgg
     5881 tctgtcaaac aatcaaatac tggcacaaat tccacctgca tataggcaga tgcaagctct
     5941 tgataggctt gattccagtt ctgaattttt tcattcgagc ggatatagac tgcctgctgg
     6001 tactcctctc cctcattgac aggcaaaatg gaaagcaatt taatctctgt caatggataa
     6061 tcgcgagcaa cggattgaat gatagcttcg agattattga gagcctcatt cacaggaaca
     6121 tcctttccga tatcatttgt cccaatcaga agaaaaattt tatctactgc tccaccatat
     6181 agatgagcat caagattctc tagtaacagt cctgtctgat agccacgaat tcctcgattg
     6241 acaatcgtct ttgaagtccc aaatagctcc tgtagaggat aatactcgac aatggaatcc
     6301 ccaataaaaa gaatgtttgg ttctacaaca gaaatgtgat ttaggtgacg atacttagtt
     6361 tgaatttttt cttgttcctt taggagccaa ttttctaata actgtactgc cacttcattc
     6421 tcctttttct aaccagtttt ccaagacttg ataaactcct ccttggctgt tggctggtgc
     6481 tagagcagtc gccacagctt tggctttctc atcagcattt tccatcgcat aggcaattcc
     6541 agccatttca agcatttcaa catcattttc actatcacca aaagccatga tttcttggga
     6601 tttcaagtcc cagcgcttga gtaattcctc caagccccat gctttatgaa tcccagcttg
     6661 gaggatatca atgcaaccat agccactgga tacagctcgg acacggccat caaagagagc
     6721 attgatttct tccaaaaccg aactcaaacg ttcctcacca acaaccatgc tcatcttgag
     6781 cacaccacca aagaggtcag atgttaattc atccacaaat tgcatccgtt ggtagaattt
     6841 ttcaatcatt tctggagtca taaaactttc aagatctgta aaaatcgtac cttccttgac
     6901 aaaatcaccc ttcatccccg ttacaacaaa ctggtcctga cacgctcgac ccttgaaatg
     6961 agccaaagcc ttgttgacaa tggcgtcatc ccatgtctga gcctgaatca attcattgtt
     7021 ttcaaaaata cgagcgccat tagcaacaac cagaaccact cgatccacca agggactcag
     7081 tagttgtctc atgcggtgaa tttcattgcc cgtcgcaatg acaaaacgaa tgcccctttg
     7141 atccaactga tctaaaatct tttccaatcg tgggagatca agctgacccc tagcatccag
     7201 caaggtccca tccatatctg tcgcaattac cttaatcatc atttactttc ctctggattc
     7261 aagctaatac cacttcaatc ccacatgttc attcatttct ttataggctg tatcaattcg
     7321 ttccttagtc gcttcatcta actggtcacg atgactgcca cccctctata aaatcttcca
     7381 aaatataagt ttggtaatga tacaagggat atccttctgg tgaaaaggtt ccctttggtg
     7441 ttcgattgac ccaagtagga tgagctttag ctgttccgat agttgttttt ccatccttct
     7501 tcttgatggt gacatccatg agaacaccac gttcagtcca cttagcattc tcttcatctc
     7561 ccatagattc aatccgttga ttggaaatga agttccccat ttaatagata atgagtttct
     7621 tatctccatc tttttcaacc gtttcagatg gttcaacaac gtgaggatgc cctccaaaga
     7681 taatatccgc tccccaatcg atcatcttgt gataaagagc tttttgttct tcagttggtt
     7741 ccaatcgata ctcaacaccc atctgaggca tgataatggt gatatctgct tccttctctg
     7801 cccgttcgac ttcagccttt atcttatctt cgtttaaatc tgaaagataa cgattatagt
     7861 cttcctgaaa aatatactgc tcaattccat tgaaaccata ggaataggct aacaatgcaa
     7921 ccttgatagc attcacttcc ttaatgacca gcggagcctg atcacgtggt tcgtgcgtat
     7981 aaactccgat tggagtgatt ccagctttct caataatatc ggccattgaa ataactccct
     8041 caatttgcga atccaaaata tgattatgag ctaaatccag cacatgataa cctgcttcct
     8101 taatagcatc cataacttca acaggagcat taaagagaag ataacccgct aaataatgat
     8161 ccttattaat ggttccttca aaatcaccaa tagctaaatc tgcttgcttg agccaaggag
     8221 tcacatactc aaaattttca tgaaagtcat aggtaccgtc ttctttttta gctgaaaaga
     8281 aaagtccatc gtggtagagc aaatccccgt gggccataat tctggcagtt ttttcctctt
     8341 cctactcctg agacttttgc ttagtcccct cttgagaaat agtatctcgt ttttgactag
     8401 tcaagggatt ccttggaagc tttcaaacaa caagaccaag cccattgata aaccaactgc
     8461 tagcaagagt atcgccacaa atcccttatt gctccacttg cgataactcc taaaaagttt
     8521 accaagccct tcataaaacg aaaagctaaa ccaccctgat ttcgattttg tcttctttgt
     8581 atcttcattc tccctacttt cttatgcaag ccttttcttt ttattatatc acagataagt
     8641 atttctttca caattgaatt gaacttccca tctattttct ataaatccta aatgccataa
     8701 tgctttcaat tcttgtcatt ttgtgatatc atgtagaggt aatgaaagga aatgaactaa
     8761 tccacagtgg cttattccaa gtataccact tgggctttgg cagtagttaa ctgcactaaa
     8821 cataatataa ggagagaaaa tgtctgcaat agaacgtatt acaaaagctg ctcacttaat
     8881 tgatatgaac gatattatcc gtgaagggaa tcctactcta cgcgcgattg ctgaggaagt
     8941 cactttcccc ctatctgacc aggaaatcat cctaggcgaa aagatgatgc aattccttaa
     9001 acattcccaa gatcctgtca tggctgaaaa aatgggactc cgcggtggtg ttggactggc
     9061 tgctccccag ttagatatct caaaacgcat tatcgctgtt ttggtaccta atattgttga
     9121 agaaggcgaa actccacagg aagcctacga tttggaaggc attatgtaca atccaaaaat
     9181 cgtctctcac tctgttcaag atgctgctct tggcgaagga gaaggttgcc tgtctgttga
     9241 ccgtaacgtg cctggctatg ttgttcgcca tgcccgcgtt actgttgact actttgacaa
     9301 agatggagaa aaacaccgta tcaaactcaa aggctacaac tccattgttg ttcagcatga
     9361 aattgaccac attaacggta tcatgtttta cgatcgcatc aatgaaaaag acccatttgc
     9421 agttaaagat ggtttactga ttcttgaata aagaaaatcc cgttgcaaga cggggttttg
     9481 tgttataata gaggcatgaa aacaaatgat attgtctatg gtgtccacgc cgttaccgaa
     9541 gccctccttg caaatacagg aaacaaactc tacctccaag aagatctccg aggtaagaat
     9601 gttgagaaag tcaaggaact agctacagaa aagaaggtgt ccatttcttg gacatcaaaa
     9661 aaatctctct ctgagatgac tgaaggtgct gttcatcaag gttttgttct acgagtgtct
     9721 gaatttgcct atagcgagct agattacatc cttgcaaaaa cacgccaaga agaaaatcca
     9781 cttctattga ttctagatgg tctaaccgat ccccataatc tgggttctat cttgcgaaca
     9841 gccgatgcga ccaatgtttc aggtgtcatc attcccaagc accgtgctgt cggagtaact
     9901 cctgtcgttg ccaaaacagc cacaggtgct attgaacacg ttccaattgc ccgagtgaca
     9961 aacctcagtc aaaccttaga taaactcaag gatgaaggct tctggacctt tggaacggat
    10021 atgaacggta ctccttgcca caagtggaat acaaaaggga aaatcgccct catcatcgga
    10081 aatgaaggaa aaggcatctc tagcaacatc aaaaaacagg tcgatgaaat gattaccatt
    10141 ccgatgaatg gacatgttca aagcctcaat gccagtgttg ctgcagccat tctcatgtac
    10201 gaagtttttc taaatagact ataaaaaagt ttccagtcat ctgattggaa acttttttat
    10261 gattaactat gttctgtgat aaacttgtag gcttcttgac cagcgataga tccatctcca
    10321 actgctgttg ttacttggcg aaggtctttc aagcgaacat ctccaactgc aaagataccg
    10381 tcgactgcag tcttcatgtg gctatctgtc acaatccagc ctgcctgatc ttggatattc
    10441 aattctttaa caaaatcgct aagagggtcc aaaccaacat agataaagac accaccgaag
    10501 gcttgctctg tcacttgacc tgttttcaca ttttcaaaaa cgacagattc tactctgttt
    10561 tcacccttga tttccttcac tacagaatcc cagataaagc tgattttttc attcgcaaag
    10621 gcgcgatctt gtaaaacctt ttgggcacga agttggtcac gacggtgaac aatggtaaca
    10681 gtcttagcaa aacgagtcaa gaagagggct tcttcaacag ctgaatctcc accaccaact
    10741 accaataaat cttggtcacg gaagaaagca ccatcacaca cagcacagta agaaacacca
    10801 cgactgttca gttcttcttc tccaggcact cccaaaggac ggtgtttaga accagttgct
    10861 acgataactg tacgtgtttc atatgtttgg tcatcagtca tcactttctt aaaatcacca
    10921 tggtcttcga cattttcaac ataaccataa atgtgctcaa caccaagatt ttcaagtggt
    10981 tcaaacatct tttcagccaa ttcaggtcca ctaatattag cgtatcctgg gtaattttcg
    11041 atatcagatg tattattcat ctgaccacct ggcagaccac cttcaatcaa agctactttt
    11101 agattgcttc gagcagcata caaggccgca gtcatccctg caggtccagc accgataata
    11161 atagtatcgt acatatagat tccttctttc ttgttgtaac tatctttatt ctaactcttt
    11221 cttgtcaatc acgcaaggat tatgccttga aaacaagaat taaactcata accgcaaagg
    11281 ataatactgg gacaaccaag actccaatca taatcatatc atagagatgg gcttggcgag
    11341 ccctggctgt cttatcaact cccgacatgg ctctcagacc aatccaaatc cctaaaaaaa
    11401 tcagaacaag gatggtggtc aagatcaaac tctcgaaata taaagaaaat agttgcagta
    11461 gcatgatttc tctcatttct atctttttta aagagtaaac tcagctagtc caactaactg
    11521 agttttcctt tatctattat atcaaatata agtccgtttg taactagcaa agaattcttt
    11581 tgtccgctct tctttcggat ggtgaataat ctcatccggt gttccagact caatgatttt
    11641 ccccttatct aggaagagaa tcttatcagc cacttgggct acaaaggaca tatcgtgact
    11701 gactaaaatc atagtctgtc ctgacatagc agcatctgca atagacttct ctacttcacc
    11761 aaccaattct gggtcaaggg ctgaagttgg ttcatctaaa agcaagacat ctggtttcat
    11821 agcaagcgca cgcgctagag caacccgttg cttttgtcca cctgataaat ggcgaggata
    11881 atggttttca cgatccgaaa gcccaacctt agccaactct tccttggcaa tcttagtcgc
    11941 ttcttggtca gataatttct tgacaacaac caagccttct ttcacattat caagtgctgt
    12001 tcgacgttca aataaattaa actgttggaa aaccatagac aacttacggc gtagggcaag
    12061 gatttcttct tgagtgattt tagaaaaatc aactgaaaaa ccatcaatct gaatagagcc
    12121 actgtcaggt gtttcaagat aattgagact gcgaagaaag gttgattttc cagctcctga
    12181 agaaccaatc aaggctacaa cttccccttt ttgaatatcc aagttcagat gatccaagac
    12241 agtctgtcct gaaaaggatt tgcttaaatt cgaaatctta atcattaacg aaggtctcct
    12301 ttcacatctg tttgcactgt atcaggtgca gaaatagcca tttttctctc gatgaaacga
    12361 ccgaggcttt caattccgat attgactacc caataaacaa gggcaacaga gatgaagcgt
    12421 tcaaaatagc gataatcagc tccacctaga atctgagctt gggcaaagac ttccacaaca
    12481 cccgcactaa aagctagaga tgttcccttg gtcaaaccga tgagggaatt aatcaaggtt
    12541 ggagtagcta ccaccgctgc attaggaata atcactcgtc gataaacttg cgctcgggtc
    12601 atacccagac tgcgcgccgc ctcaatctca ccaggattaa ctgagagaat ggctgcacga
    12661 atggtttcac tagcataagc tgcctcatta aaggcaaaag cgacaatcgc aaaagctgca
    12721 gctggaatcg cattgatatt gagaccagtt ccccattgct gattgagggc tttcaaagcc
    12781 aaagggattc cgtagtaggt caacatgagt tgcaccaaaa tcggtgtccc ttttaagaaa
    12841 ctaacaaaga aggcctgcaa gggatataaa atcttgacac gattgatctt cacaatggca
    12901 aaaagaagcg ccaaaaccaa gccaaaaagg gcaccaccaa ttgtcaacat aattgttgtt
    12961 ggaagttgtt ggacaattct agggattcca tcaaagaccg aacgtaggct aaacagcttg
    13021 ccatccggaa tcaattgcat caagttttgg taccaatctg atgctaaaat cgttgtaaca
    13081 ttcataaaaa catcctcttc tgataatgat tcattctacc atgcttctgc cgtcaatgaa
    13141 attatttgct acggtcagat acagactttg tctacctact agtttatcat actttgaaac
    13201 agggaagtta tatattttat ctatcaaaga gatagataaa aactatttca gtcccagttc
    13261 ttcataagat tttcgataac cgatttgctt aacagttcca ttttctacta aaatgggccg
    13321 ttttaacaac ataccgtcac ttgctagcaa ctcagccgct tcttggtttg acaaacttcc
    13381 taccttatct tttagcccta attcacggta tttgatacca ctggtgttga aaaattgctt
    13441 caattcaaat cctgaggttt ctagccaatt caaaatgact tcttggctag gtgtttcttc
    13501 cacgatatgg acggctttat agtccacacc taattgattt aattcttgtt ttgctttttt
    13561 acaagttgaa catttggggt attcgataaa ttctaacatg tgcttctcct tctatttgtt
    13621 tatatcttta aaatctacgc cttcatgctc caagagccaa gctttctttt ccactcctgc
    13681 agcatagcct gtcagacgct tgcctgctcc caacacacga tgacaaggta ctaggataga
    13741 ccaaggattg cgtcccactg ctccaccaat tgcttgagca gaagccactt gcaggtcttg
    13801 agcaatttgt ccataggtca ctgtctgacc ataaggaatg ccctgtaaat agtcccaaac
    13861 tctcttttca aaattcgttc cgattggcgc caagagcaag ttggataaat cctgaggctt
    13921 gcctttaaag taatcatcta agcaagcaat aactgggtct aaaataggat gactaacaac
    13981 ttcttctatc gtttcatctc ctagtcccct ctcaaaatgc ttctgctcct gaacccaaat
    14041 tccatacaaa taatggtcat cagctacaag tgatagaata ccaattggtg aggagtagag
    14101 tatttttaca tacttctttt gcattatttc tttaattttt gataggccaa gcgttcgcca
    14161 tctactggca ccttaccaac ttgtttaaaa ccaagttttt caaaaatatg ttgcataacc
    14221 ttgttttcag catgcgtatc tgagcgaaaa tcaagataat caaaaccttc aatcaagccc
    14281 tctaagaagg tttgagcaac tccttttccc tgcacatctg ctgccacagc aatacggtga
    14341 aagactagat actctgactc tccagcttgc cagtttccct cataaatagc ttcataggcc
    14401 tcctctggac tcttggtcac agcagcatag gctagcagtt ctccctcttc caaggctacg
    14461 taggcttgcc ctgagatgat atcatcgata ataacatctg catttggata gccattttgc
    14521 cactggtcac taccagcatc tgctaaacat tttttggcat cctccatcac ctgcatgatg
    14581 gcatctactt catttggaaa agctaaacga atttccatct tttttcctca tttctgacta
    14641 gtttctccca tcatagcata atttaagtat ttctacaagc agttaaaact tgactttgtg
    14701 tttttgttat tttataatct agttatcaaa actatataaa aaggagttga aaatgaaaac
    14761 taccttttcc tacccaaaat gggcagaaat tccaaacatt gacctctatc tggaccaggt
    14821 tttgctctat gtcaatcagg tctgcgcccc tatctctcct aataaagaca agggcctaac
    14881 agcatctatg gtcaacaatt atgtgaaaaa tggttacctg acaaagcctg acaaaaaaaa
    14941 ataccaacgc caacagattg cccgtttgat tgctatcaca accctcaagt ctgtattttc
    15001 tattcaagaa atagcccaga cacttaatac tctacaaact caagcaagtt cagaccaact
    15061 ctacgatgct tttgtggact acatgaacca aggaattgac ccagctaacc ctattatcca
    15121 aaccagctgc caaaccgtca aactctatca tcaaactcta gacttaatcg atcatactca
    15181 agaggaggta atccaatgaa cactagtctt aaactcagca aacaactcag ttttggagag
    15241 gagattgcta atagcgtgac ccatgctgtg ggtgcagtca tcatgcttat cttgctgcct
    15301 atttcatcca tctatagtta tgaagcacac ggatttttat cctctatcgg cgtttccatt
    15361 ttcgtcatca gtctctttct catgttccta tcatccacca tttatcactc tatggcctat
    15421 ggttcgaccc acaaatatgt tttgcgaatc attgaccatt ctatgattta cgttgccatt
    15481 gccggctcat acacgcccgt tgtcttgacc ttgatgaata actggtttgg ctatctgatt
    15541 attgtcatcc aatggggaac gaccatcttt ggtattctct ataaaatctt tgctaaaaag
    15601 gtcaatgaga aatttagcct tgctctttac ctgattatgg gctggttggt tctggctatc
    15661 attcctgcca ttatcagtca aacgacaccc gttttctgga gtctcatggt aactggcgga
    15721 ctctgttata cagttggagc tggattttat gccaagaaaa aaccttattt ccacatgatt
    15781 tggcatctct ttatcctagc tgcgtccgca cttcaataca tcgctattgt ttattacatg
    15841 taaaaaagtt gagaaattca atctcaactt ttttctttac acatattgat aaagtactgg
    15901 tgcaagcgca catcatcagt caattctgga tgaaaagaac ttaccaacat atttttttct
    15961 tgggctgcaa caatttgatt gttcactgtt gctagaattt ctacaccctc accaacacta
    16021 ctgataatcg gaccacggat aaaggtcatt ggaatcttgc caactccctt acattctgct
    16081 tccgtgtaga aacttcctaa ttggcgccca taagcattac gctcgaccac catatccata
    16141 gttcctagat gactctcttt ctgagaagtg atttccttag ccagcaaaat taagcccgca
    16201 caggtcccaa acactggtaa gccagataga atggcttctc ggatgggaag tagcatgttc
    16261 tggtcacgta agagcttgcc catggttgta gactcaccac caggcaaaat caaacccgac
    16321 aagtcactct gatcttgctg aaaatcatct agatttctga gttctacact ctcgacacct
    16381 aattgatcta gcacttttgc atgttctgca aaggcccctt gcaaggccaa tattccaatt
    16441 ttcatctatt ttcctcgttc agccatgaga atttggattt cattttcatt aataccaacc
    16501 atggcttctc ctaaatcttc agagatttga gctaggattt gaggattacg gaagttagtc
    16561 acagccttaa caatggcact cgctcgttta acaggatctc ctgacttgaa aatacctgaa
    16621 ccgacaaaga ccccctctgc ccctaattgc atcattaacg cagcatctgc tggcgttgca
    16681 acacctccag cagcgaaatt tacaactggc aattttccat gttcatgaac atattggacc
    16741 aattctacag ggacttgcaa atccttggca gcaacataaa gctcgtcctc acgtaagttt
    16801 tgaatgcggc gaatttcctg attcatcata cgcatatgac gaacagcttg gacgatatcc
    16861 cctgtccctg gttctccttt ggtacgaatc atggaagcac cttcagcgat acgacgcaag
    16921 gcttcaccca aatccttagc accacagaca aaaggaactt ggaattcttt cttgtccaca
    16981 tggaaacggt catcagctgg agatagaact tcactctcgt cgatataatc aatttcaata
    17041 gcctctaaaa tctgagcttc aacaaaatgc ccgattctga ccttagccat tactggaata
    17101 ctaaccgctt cttggatttc cttaatcatc tttgggtcgc tcatgcggga aactcctcca
    17161 gctgcacgaa tatcagccgg aattcgttcc aaggccatca cagctgccgc accagcagct
    17221 tctgcgatac gagcctgttc aggattctgc acatccataa taacaccacc cttgagcatc
    17281 tgtgccaagt ttttatttag ttcataacga ttttcagtca tttgttttat cctcatcatt
    17341 tgtattggta gctacctttt cattttaagt cagaacagaa tgaagcacaa gataaaaaaa
    17401 ctataattgt atggggacag attggctaaa aactatctga ccttaactta aggccagata
    17461 gctcattcat ttttattttt cagccgtaag ggcagccatt gtgatgtagt tgtatggttt
    17521 attgaagtgt ggcaagaaga agaggtctgt caatgccaat ttatcaattg tcacatgctc
    17581 ttggatagca agtgagaaca tgtggattcc catgctaatt gcaatatcat gtgaaaccat
    17641 ttgagcacca agaatttcac ggctatcttt gtcaaagaca atcttaattg ctacttcatg
    17701 gttgtcatgt ttcatgaatt ctggtttttg aagatcgtta aagcctgttt cagttgcgtt
    17761 gtaaccagca gctttcgctt tttcaagagt caaaccagtt gaaaccatgt gaagaccgta
    17821 gattgagata ccatttgaac cttgaacacc gattccttcc aattcatgtc cacaagcgtt
    17881 gtaggcacca acgataccag tgcgaacagc atttgaagca agagcgatat agcttgtatc
    17941 tttacgagca ttgtcataaa cagtcgcaca gtcaccaaca gcgtaaacgc ctgggataga
    18001 tgtttcttgt ttcttgtcta caaggaaggc accgttgcgg aagagttcga tcttaccatc
    18061 agcaagggct gtgtttggac ggaaaccaac tgcaaggata accatatcca cgtcaaagct
    18121 ttctttgtca gtaatcaagc gttcaacttt accatcacct tcgattgctt taacagtttg
    18181 acctagagcc aagcggatgt tgtgatcttc caagttcttc gccatcattt gtgtgaagtc
    18241 tttgtcatag taaccgttca agacagtatc aacgatatca acaaggacaa cttcttttcc
    18301 aagacgttca aaggcttcag caagttcaac accgatgtaa ccaccaccaa caacggcgat
    18361 acggtcgaga tgttggctct tgtcagaaag tttattgata acttcttcag cattttggta
    18421 caatttcacg aattgtacgt tttcaagagt tgctttaaat tcgcggtttc ctttaacaat
    18481 ttcaacacct tcgattggtg gcaagattgg tgtagaacct gtagcgaaaa tcaatttctc
    18541 gtatgattct ttgtgttctt ttccttcaac ttctgctgta accactttgt tatcatagtc
    18601 gattgaaaga actggtgaat tcatataaac cttagcacct ttagcttcta atttgtcttt
    18661 atcagaatag aacaagcctt cagcaccgtc aatttgttca ccaatccaaa gagccattcc
    18721 acatcctagg aaagagatgt tagagttttg gtcaaataca acaatttcgt tctcatttcc
    18781 aaaattatcc aacatggtat tgatacatgc tgtaccagcg tggttagcac cgactacaac
    18841 gattttactc ataaaaaatt tcctgccttc attctaaatt tactttacta gtataccatt
    18901 taccgttagc gtttacaagg gggggactat tatttcagca ttttttatca aaaataattc
    18961 cccatttatc ctgttttcaa gcctcatgaa ttttatgagc atatttcttg actttttcca
    19021 aattttgttt gtgaaaattc taactttatg gtattataat aacatgaaaa taaaatgtaa
    19081 gggcttcaat ttagatgatt atttgatatt gcaacttatt ttctataaga gaaaggagtt
    19141 ggtagcttga ctcttcgttc acattctgaa cggctaatgg ggactactat cactatttca
    19201 ttagtggatg agcaagccga tatctttctc caaaaatcct ttgacttgct caaagagctt
    19261 gaataccgct tcaacgccaa tagtcaagaa tctgagttga tggaaatcaa ttatcaagca
    19321 ggaatatccc ccgtcacagt tcatccagac ctgtttgagc tgatttcact tgggttagag
    19381 catagcctag cgctctctag ccacctcaat atcagcattg gtcccttaat tcaaacctgg
    19441 cgtatcggtt tttcagatgc caaggtcgcc cagcctcaag aaattgaatc ggtgctgcct
    19501 ctaatcaatc ctcatggtat cgagttagat tcttctactt ctactgtgtt tttaaaacag
    19561 aaaggaatga agatcgatct aggttgttta gccaaaggat acagtgcgga taaggttgcc
    19621 caatttctta ggaaagaggg ggtgacttct gccttgatca atctgggagg gaatatcctg
    19681 accattggaa aaaatcaggc aagaggggat aacccatggc aaatcgggat tcaagaccca
    19741 gccaatccta ggggaaatca tttaatgacc atccctgttg tcaataaatc tgtcgtgact
    19801 tcaggcattt atgaacgtca cctgaccgtc gatggacaag attaccatca catttttgac
    19861 agtcaaacag gatatcctgt tgaaacggaa ctagcgagtc taacaatcat ctctgataaa
    19921 tcagtcgatg gcgaaatctg gacgactcga ctctttggag aaagacctgc ttctatcctc
    19981 tggcaagtcg aaagtttgga gggcatcgaa gtcatcctca tcgataaaga aggccaccta
    20041 agctgttctt caggaattcc tactctatag agacaactgt ttcaatggaa ttttaaaatt
    20101 gtattatgta aaaagagaaa ggaaatatca tgttaaaact tattgctatt gttggaacaa
    20161 attcaaaacg ttctacaaac cgtcaattgc ttcaatacat gcaaaaacac tttgctgaca
    20221 aagctgaaat tgaacttgtt gaaatcaaga ccattcctgt cttcaacaaa ccagctgaca
    20281 agcaagtacc tgctgaaata ttggaaattg ctgctaaaat cgaagaggca gatggcgtta
    20341 ttatcggtac tcctgagtat gatcactcta ttccagctgt tttgatgagc gctcttgctt
    20401 ggttgtctta tggtatttac ccacttttga acaaaccaat catgattaca ggtgcttctt
    20461 acggtacgct tggttcatct cgtgcccaat tgcaacttcg tcaaatcttg aatgctcctg
    20521 aaatcaaggc aaatgttctt ccagatgaat tcttgctctc acactctctt caagcattta
    20581 acccaagtgg cgacttggtt gaccttgatg ttatcaagaa attggatgcc atctttgatg
    20641 acttccgtat cttcgtgaag attactgaga aattgcgcaa tgcgcaagaa ttgcttcgca
    20701 aagatgctga agactttgac tgggaaaatt tgtaagatag gagaccgaaa gaatgaaatt
    20761 tgttggactt gttggatcaa actacgatca atcatataac cgcaaactct tggaatttat
    20821 ccgtcgcaat ttcaaattca aatttgaatt agaagtactt gaaatcgacg aagttccaat
    20881 gtttaaccaa gacgaaaaat gggacgaaag cttccaattg cgtttcttgt ataacaagat
    20941 tacacgtgct gatggtgtca ttatcgccac tcctgagcac aaccacacta tctcagcttc
    21001 actcaaatct gtacttgaat ggctttcata cgaagttcat ccatttgaaa acaagcctgt
    21061 tatgattgtg ggtgcatcat actatgacca aggaacatca cgtgctcaag ttcaccttcg
    21121 taaaatcctt gatgctccag gtgttaatgc ctacacgctt ccaggtaacg aattccttct
    21181 tggtaaagct aaggaagctt ttgataacaa cggaaatatc accaacgaag gcactgttaa
    21241 attccttgaa acttgcttag ataactttgt taaatacgta ggagtcgttt cgaaattgaa
    21301 aaaaccaaaa ccaatcgaat cagaagactt ggattgtgga aaaccaatcg ctacaaccat
    21361 cacagaagtt gaccctgacg atccagaatg ggtagaaaaa gttgcagcaa tcactggtgc
    21421 tgtttctggt gatacctatg tcaaattgga ccacggtatc ctaacagtta accaaattga
    21481 tatgttcttg aaagctatgc catttgaatt gacatacgct gacgacaaca accaattcct
    21541 ctactacaac aacgctcacc aagatccaga cactatgttc gctaaacgtg taccacctca
    21601 atcaggtagc cgtatgtcga ctgttcatgg ttctcttcca ccagcacgta tgaagaacgt
    21661 agagtgggtt atcggaacac ttcgcaacgg aaaccaagaa tacgtccgta cgatcgttcc
    21721 aggttctcct gcaggtgtta tcaacactca caactaccaa gcaatgtact atcctgatgg
    21781 atcatacgct ggtatcaatg aaatcgtctt taacttccaa ccatggcttg actggtacct
    21841 aaaagagact ggtcaacgtt tggtgggtgg tagcggacca tttgctcctg ctgccggagg
    21901 tcatggagac gccgatgcta cttctggcgc ttctgattca ggggatgctg gaggccacgg
    21961 tggtgacgca gacgctacag ccggtgcaag tta
//
LOCUS       DAVBGY010000032        21972 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVBGY010000032 DAVBGY010000000
VERSION     DAVBGY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21972
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.032"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(408..1604)
                     /gene="tuf"
                     /locus_tag="VQ620_001236"
     CDS             complement(408..1604)
                     /gene="tuf"
                     /locus_tag="VQ620_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2763857.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2515..3384)
                     /locus_tag="VQ620_001237"
     CDS             complement(2515..3384)
                     /locus_tag="VQ620_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET2763858.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3607..4287)
                     /locus_tag="VQ620_001238"
     CDS             complement(3607..4287)
                     /locus_tag="VQ620_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2763859.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPEIPSPQEPGTPGEPTPEKPIPQPNPEHPSVPTPNPELPNQETPTPDK
                     PTPEPGTPKAETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAGLGLGF
                     FKKKEDEK"
     gene            complement(4245..5351)
                     /locus_tag="VQ620_001239"
     CDS             complement(4245..5351)
                     /locus_tag="VQ620_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET2763860.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSYRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5462..5716)
                     /locus_tag="VQ620_001240"
     CDS             complement(5462..5716)
                     /locus_tag="VQ620_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763861.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQV
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(6074..6607)
                     /locus_tag="VQ620_001241"
     CDS             complement(6074..6607)
                     /locus_tag="VQ620_001241"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET2763862.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPVLYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVMDLL
                     REVPTIFECLECFFKTR"
     gene            complement(6594..6809)
                     /locus_tag="VQ620_001242"
     CDS             complement(6594..6809)
                     /locus_tag="VQ620_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2763863.1"
                     /translation="MGKERREQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCAVPSVWAALKQIKVTLKKDDEL"
     gene            complement(6776..6919)
                     /locus_tag="VQ620_001243"
     CDS             complement(6776..6919)
                     /locus_tag="VQ620_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HET2763864.1"
                     /translation="MAYSTDFKQRALDYIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            7153..8871
                     /locus_tag="VQ620_001244"
     CDS             7153..8871
                     /locus_tag="VQ620_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET2763865.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            8982..9329
                     /locus_tag="VQ620_001245"
     CDS             8982..9329
                     /locus_tag="VQ620_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET2763866.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(9608..10444)
                     /locus_tag="VQ620_001246"
     CDS             complement(9608..10444)
                     /locus_tag="VQ620_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2763867.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(10457..11086)
                     /locus_tag="VQ620_001247"
     CDS             complement(10457..11086)
                     /locus_tag="VQ620_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2763868.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLDNLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(11096..11737)
                     /locus_tag="VQ620_001248"
     CDS             complement(11096..11737)
                     /locus_tag="VQ620_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2763869.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLIPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(11883..13112)
                     /locus_tag="VQ620_001249"
     CDS             complement(11883..13112)
                     /locus_tag="VQ620_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET2763870.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(13102..14268)
                     /locus_tag="VQ620_001250"
     CDS             complement(13102..14268)
                     /locus_tag="VQ620_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET2763871.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            14412..15425
                     /locus_tag="VQ620_001251"
     CDS             14412..15425
                     /locus_tag="VQ620_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HET2763872.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVRMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(15470..15889)
                     /locus_tag="VQ620_001252"
     CDS             complement(15470..15889)
                     /locus_tag="VQ620_001252"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET2763873.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(15900..17306)
                     /gene="atpD"
                     /locus_tag="VQ620_001253"
     CDS             complement(15900..17306)
                     /gene="atpD"
                     /locus_tag="VQ620_001253"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET2763874.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(17392..18270)
                     /locus_tag="VQ620_001254"
     CDS             complement(17392..18270)
                     /locus_tag="VQ620_001254"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET2763875.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(18286..19791)
                     /locus_tag="VQ620_001255"
     CDS             complement(18286..19791)
                     /locus_tag="VQ620_001255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET2763876.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQSVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(19806..20342)
                     /locus_tag="VQ620_001256"
     CDS             complement(19806..20342)
                     /locus_tag="VQ620_001256"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET2763877.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(20342..20836)
                     /gene="atpF"
                     /locus_tag="VQ620_001257"
     CDS             complement(20342..20836)
                     /gene="atpF"
                     /locus_tag="VQ620_001257"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET2763878.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(20850..21566)
                     /gene="atpB"
                     /locus_tag="VQ620_001258"
     CDS             complement(20850..21566)
                     /gene="atpB"
                     /locus_tag="VQ620_001258"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET2763879.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(21601..21801)
                     /locus_tag="VQ620_001259"
     CDS             complement(21601..21801)
                     /locus_tag="VQ620_001259"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HET2763880.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(21876..>21972)
                     /locus_tag="VQ620_001260"
     CDS             complement(21876..>21972)
                     /locus_tag="VQ620_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2763881.1"
                     /translation="LDYKSPREDFWMANLNLKFSKMEIIFIKRFQ"
BASE COUNT         6533 a         4731 c         4014 g         6694 t
ORIGIN      
        1 gatttcttct ttagttgatt tcatattact attgtataat gctttttgat ttgagtctag
       61 tattatactc ttcgaaaatc tcttcaaacc gcgtcagctt cgccttgtcg tatagatgtt
      121 actgacttcg tcagttctat ctacaaccta aaaacagtgt tttgagcagt ctgcagctaa
      181 ctttctataa caaaatgaaa taagaacaag acaaatcaat caagacaatc aaatcgattt
      241 ctaacaatgt tttagaagta gatgtgtact attctagttt caatatatta tagtttgctc
      301 tttgattttc attgagtatt agttcaatag ctaaaaaaag aaccttgcca agcaagattc
      361 ttttagtgtc tgacttaaat aattgttctt ctgggaacta aatcgaatta agcttcgatt
      421 tctgtaacca tacctgaacc aacagtacgt ccaccctcac ggatagagaa tgtagtacct
      481 tgttctacgg cgattgggtg aatcaactca acatcgattg tcacgttatc accaggcatt
      541 accatttcag tacctgctgg aagttcgatt gaacctgtaa cgtcagtagt acggaagtag
      601 aattgtggac ggtagttgtt gaagaatgga gtgtgacgtc caccttcttc tttagtaagg
      661 atgtagactt cacctttgaa tttagtgtgt gggttgattg aacctggttt agcgataact
      721 tgtccacgtt cgatttcatc acgttgaaca ccacgaagaa ggacacctac gttatctcca
      781 gcaagacctt cgtcaagttg tttacggaac atttcaacac cagtaacaac tgctttttga
      841 gtttcttctt tgataccaac gatttcgatt tcgtcgttga ctttaacgat accacggtcg
      901 atacgtcctg aagcaactgt accacgtcca gtgattgaga atacgtcctc gactggaaga
      961 agcaatggtt tgtcagtgtc acgttctggt tctgggatat actcatcaac tgtgttcatc
     1021 aattccataa cgatgtcttc gtatttagag tcaccttcaa gagctttaag tgctgaacct
     1081 tggataactg gaagatcgtc acctgggaag tcgtattctg acaataggtc acggatttcc
     1141 atttcaacca attcaagcaa ttcttcgtcg tcaaccaagt caactttgtt catgaagacg
     1201 ataaggtgtt taacaccaac ctgacgtgaa agaaggatgt gctcacgagt ttgtggcatt
     1261 ggtccgtcag ttgaagctac tacaaggata gctccgtcca tttgagcagc accagtgatc
     1321 atgtttttaa cgtagtccgc gtgtcctgga gcgtcgatgt gagcgtagtg acgtttttca
     1381 gtttcgtact caacgtgcgc agtgttgata gtgataccgc gttcgcgttc ttctggagca
     1441 gcatcgatag acgcatagtc tttaggttgg ttaactgatg aaggcaagcg acgtgccaaa
     1501 acagttgtga tagctgcagt tagggtagtt ttaccgtggt caacgtgtcc gatagtacca
     1561 atgttaacgt gtggtttact acgatcgtat ttttcttttg ccatttgagt aaaagcctcc
     1621 aataaaatat attttataga tagacagtag gcaatacagt ctaactttcc ttactatttt
     1681 atcaaatttc agctaaattg caagtgtttt acaacgtttt tgtgaattat tcttggtaaa
     1741 aatttaacta attaatagaa agtactaaat agacttatcc ttcaaggagt tttcagcatt
     1801 atccatccac tcacttgctg aaaatgtaaa cagtctctgg tttatatagt aaaatgaaac
     1861 aagaatagag caaatcgatc aggacagtca aatcgatttc taacaatgtt ttagaagtag
     1921 atgtgtacta ttctagtttc aatctactat acaacctcaa aactgtgttt tgaacaacct
     1981 gcggctagct tcttagtttg ccctttgatt ttcattgagt ataaaattgc agataaaacg
     2041 ataacaatca taggactcag cagagtcaat gtaggtcgaa gaatagcccc cctaatcgct
     2101 tattttatag caaaatgaaa tagaaactga acattttgtt gaggaaggta aatcaattcc
     2161 tcacaatgtt tgagaaagaa gggaggacta ttccaacttc aacccactat aaagcagact
     2221 tacaagatag ggaacccatc tccgataata tatagtagaa tgaaaactgc acaatttgat
     2281 tggagaattc aaaccaattt ctaacaatgt tttagaaaca agggtctatt atttcaactg
     2341 caacctacta tagcccattg aagctggaat agcccactga gacttctaaa ccactgctgg
     2401 agaggagttt gtttagagtt tctttcattt ccctaaaaaa tcaaaaagag agaaccagtc
     2461 tgatcctctc ttacttatag atggtgctca ctcaaatgtt aaaggagcta tttcttagag
     2521 ataaaggaat ttgaatacag ctactgccgc aattgctgct gcgataggtg ctactactgg
     2581 tacccaagaa taccaccatt ttgaatcgcc tttatgctca ccaagaactg attttggaag
     2641 gaaagcatga aggagacgtg gtcccaagtc acgggctggg ttcaaggcag gtcctgtagg
     2701 tcctccaagt gatgttacca aagccataac gaggaatcca agtgccaagt gagccacaga
     2761 aagtcctgaa gcagtgtgtg gagccacctg tgctttaata gccaagtcag aaaaatcaac
     2821 tgtttgtcct gcttctattg ccttttgttt catgaattga agcacttcag caccaaagaa
     2881 gtttttagtc aaaccaagag ctgcaaagaa caaaacaaat gaaccaacaa actcattaat
     2941 caaaccattg acagttgctg catagcgact ttcttttgta ccatggtcaa tacttgaaat
     3001 agttgagaaa gttcccaaga tgttatttgg tttttcagtt ttcaagtaga atggacggta
     3061 tgttgccaca actaaggctt ggccaaagat agcccccaag acttgcgcga taatgtaagg
     3121 taccacttgt gcccaaggga aaagaccgct aactgcaagc cctagagtga aagcagggtt
     3181 gatgtgattc ccagatacgt taccaaacat caaggctggg atcataaccc ccataccata
     3241 accaacagcg atgacgatcc agccactttg gtgacctttc gtacctttaa gttcaacgtt
     3301 ggcaactgca ccattcccaa gaatgatcaa aatggcagtt cccaaaaatt cagtggcata
     3361 cttcagtgcc catgtgaaat ccatttgcta gattctcctt aaaatttttt agatattcct
     3421 tattttatca atttttaccc tatttagcaa ctgatttctg gaaatatttt taaaaaaata
     3481 ggattcatag tttcccagtt caacgtactt gtaagcgttt ttattcttca cgaaagacct
     3541 cattctcaaa accaggaaaa agcccacatc tgtgatcttt tcctggttcc tagattctaa
     3601 aatctattat ttttcatctt cttttttctt gaagaatcct aatcctagac cagctaacaa
     3661 ggtcatgata ccagcactag ccaaggtagc attagcttct gtacctgtgt ttggcaattc
     3721 ctctctctta cctgtctcat aagtcggaac ttctgggtct ggattcactg gagtttcagc
     3781 ttttggagta cctggttctg gagttggttt atctggtgtt ggagtctctt gatttggtag
     3841 ttctgggttt ggtgtcggaa cacttggatg ctctggattt ggttgtggga ttggtttttc
     3901 tggagtcggt tcacctggag tacctggttc ctgaggactt ggaatttctg gtttttctgg
     3961 agttggttta tctggtgttg ataaacggtc ataccttacc gttatttctt tatcactaga
     4021 gtctgacgta acttcttgtg attcaactgt tggaatatct ggatctttgt acttgtcaat
     4081 cttaccagat ataacctcgt cccagtttcc tgttgtccat tcaccgtagg ttacaactcc
     4141 cgtgaccttg ttctcagttt ttgtacggct taaggttaca ggttgaacaa catcttcttt
     4201 tacattttgg ttcgtaactt tatcaacgta atgaatgata cgcgttatag tcttcgtctc
     4261 agtagaggtt gctgttttgg gaaccactgt ttcctcaaca ttctcacggt agtaatagtc
     4321 aactgttgca ccgtcttctg gtacgcattt gcaggagttg ctaccaaggt gtatgttgtt
     4381 ttccttgtga taactcggtc ttctttgtcc tcagttgttg ttttcccttc aatagttttt
     4441 gattctgtgg tatactcaga acctatcgct aaatcagctt ttataacaga ctctgccaac
     4501 ttctcttggc taccttcttt atagtaattg gctgttactg tagcagtggt tggcgcttcg
     4561 ctttctctat aaactaaggt cactgttcta ccttcgctta caatattccc agttaaactt
     4621 gcagaatttg tatctgcttc tttaaaagta taatattttc cgtcagtagt agtcatgcta
     4681 ctgagttttt tatctgtgac attgtagctg gtaccaatca gttgtttttt attggtaatg
     4741 taggttccgt cactttcttt ttctccaatt ccagtatcat ttttcaatga tagcaaacgc
     4801 ccttgttcat caacatagcg aactttcaca ttttctgaga ttagttctgc caattctgag
     4861 gtttttttct ttttcttgat ttcttcggtt attttccctt tctcttcttc gggaatattt
     4921 agttttggaa tgattttttc aacaacggtt cgtgatggtt ccacagtatc ttggatgact
     4981 gaaaagtcag ctagaattgg gagattatac tgaacacggt aactttgagt gtttactcct
     5041 actctttcat tattctctga aaatacttgt acggtataag aaacaacatc ttttcctaat
     5101 agaacatccc cagtagagaa atagccgcct tttcctagtt tgctatctcc agagcccact
     5161 tctttcctaa ttttatcaga tagtttttta ccagtcagta cattcgttcg cacaatccct
     5221 ttgtctaccc ctacaaagtg ggagaacttt ttgaactctt cagaaccaga tctagcccaa
     5281 ccattattaa gggcatttgc ttttgtattt gtattctctc tcaaaggttt ggcgattaga
     5341 attatattca tcggcactta gagttgctgc tatatctgac tcttgaatac caacttcctt
     5401 actaccattt ctagcggcag tatatgtgta tttatctgtt tctacttcta gctgattatc
     5461 cttaacatga taggtaattt tctcctggta taagagtctt cagtagttta tatactctgt
     5521 catcatcttt agctttagaa gcagctgagt ttagccaagt tggggtatta ggagtatctt
     5581 tatcaacctg tttcaagaca ttattcaagt catttgctag cctattggtt atcctcactg
     5641 tgaaagtttt cttccagtct gaaccacttt tattagactt agatatgctc atgaaatcct
     5701 ggtcaacatt caacacaata tcgccttgtt ttttagcatc ttccgttgct tctgcttcaa
     5761 attgatcatg gagtttttgt tgctctggtg tatagttttt ttatttacac gatttaatgt
     5821 actatttttt attttagtat taagaactcc attaatacga tttttaaact ctgcttcaaa
     5881 aaatccctta atttgttggt gcgcaagttc aacattcaaa cctttcttat acaagtcttc
     5941 attctgatac aagtcttcat tctgaacgat ttcggctaca cggtgaatgg tttgatcgta
     6001 taccgtcttc tcttaaccag cagtatcctt agttttaatg actactcgat aggttccttt
     6061 agaaccgtta tagtcatcta gttttaaaaa agcattccaa acattcaaaa atagttggaa
     6121 cttcccttaa taaatccatc actttctttt tcaagatagc ccaagcttgc tcaataggat
     6181 tcaaatccgg tgaataaggt ggtagaggta agaaaaagtg tttatcttgg atgcagagtt
     6241 catccaaaat gttcttggga tgaaaactag cattgcccat gacaataaca tgaggtgtct
     6301 tcaaagcagg taggagttgc gttttgaacc actccacaaa gaaatcgctt gtcatgcttt
     6361 tcttgtaaat catgggagct ataaactctc cgtctacttg tcctgcaaca attgaagttc
     6421 gctcaaaacg acgtccgcta atcttttcat agactttctc ccctctagga gcccctgcat
     6481 aaggacgata gaggtagcgg tcgattcctg tttcatcaat atataggact ggtaaatctt
     6541 ttaggttatc caaaatatca agaaactcag cgactttctc tggctcttgt tccttaaagc
     6601 tcgtcatctt tttttaaagt gaccttaatc tgctttaaag ctgcccatac tgaaggaaca
     6661 gcacaatcaa aatgtgccgc aatttcccgt aaaaaagcat ctggatgagc ctctacaaag
     6721 gctttcaatt cctctaaagg aatctttcgg tttttgacga ctcgcttttt cctctctatg
     6781 tgtccttgtt cacgtctttc ttttcccacg tgaagagagt tctgacgcca acaccaaaaa
     6841 actttgctgc ctcgacatgg ctgtgcccct ctttgatgta atctaatgct cgctgtttaa
     6901 aatcagtact atatgccata gctttattat aatatgcttg tttaaaacta agcaactata
     6961 tcaacgtttt gttatgagaa gacttacttt ttttactgaa attgaatttt tcctagcctc
     7021 aacatcatat aaccccaaaa ttcaatctga cctggtttga agagattttc gaagcagata
     7081 aggttttact aaaactaaaa aaaatgatat aatcaaactg attaaatgtt ttaaaactaa
     7141 aggagaattt gaatgtctta ccaagaaaat taccagaaat gggttgattt tgtagagctt
     7201 cctgactacc ttcgtcaaga tttggaaaat atggacgaaa aaactaagga agatgccttc
     7261 tatacaaatc ttgaatttgg tactgcaggt atgcgtggct tggttggtgc tggtacaaac
     7321 cgtatcaaca tctacgttgt tcgccaagct actgaaggat tggctcgttt gattgagtca
     7381 aaaggtggaa acgagaaaga acgcggtgta gcaattgcct acgatagccg tcacttctca
     7441 cctgagtttg cctttgaatc tgcggcagtt cttgctaaac acggcatcaa atcttacgta
     7501 tttgaaagcc ttcgtccaac tccagaacta tcatttgcag ttcgtcatct caactgtttc
     7561 gcaggtatca tggtcacagc cagccacaac cctgctccat ttaacggtta caaggtttac
     7621 ggtgaagacg gtggacaaat gcctccacac gatgcggacg ctttgactac ttatatccgt
     7681 gcaatcgaaa acccatttgc agttgaagtt gctgatgtgg aaactgaaaa agcttctggc
     7741 ttgattgaag ttatcggcga agctgttgac gtagaatacc ttaaagaggt taaggacgta
     7801 aacatcaacc cagccttgat tgaagaattt ggtaaagaca tgaagattgt ctacacacca
     7861 cttcatggta ctggtgaaat gttggctcgt cgtgctcttg cccaagcagg atttgactct
     7921 gttcaagttg ttgaagcgca agcaactgct gacccagact tctcaactgt aacatctcca
     7981 aacccagaaa gccaagcagc ctttgccctt gctgaagaac ttggtcgtca agttggtgca
     8041 gatgttcttg tcgcaactga cccagacgct gaccgtgttg gtgttgaagt tcttcaaaaa
     8101 gatggtagct acctcaacct ttcaggtaac caaatcggtg ctatcatggc taaatacatc
     8161 ttggaagctc acaaaaacgc tggaactctt cctgaaaatg ccgctctctg caaatctatc
     8221 gtttcaactg acttggtaac gaagattgct gaaagctacg gcgcaactat gttcaacgtc
     8281 ttgacaggtt tcaaatttat cgctgaaaaa atccaagaat tcgaagaaaa acacaaccac
     8341 acttacatga tgggatttga agaaagcttc ggttacttga ttaaaccatt tgtacgtgat
     8401 aaagatgcca tccaagccgt tcttgtcgtt gctgaacttg ctgcctacta ccgttctcgt
     8461 ggtttgacac ttgctgacgg tatcgaagaa atctataaag aatatggcta ctacgcagaa
     8521 aaaacaatct ctgttactct ttcaggtgtc gatggtgctg aacaaatcaa agcgattatg
     8581 gctaaattcc gcaacaatgc tccaacagaa tggaacgcaa cagctatcac tgtcgtagaa
     8641 gacttcaagg cacaaactgc tactgttgct gacggtactg ttacaaactt gacaactcct
     8701 ccaagtgatg tgttgaaata cacacttgct gacggttcat ggattgccgt tcgcccttca
     8761 ggtacagaac caaaaatcaa gttctacatt gcagttgtag gggaaaccaa cgaagaatca
     8821 caagctaaga ttgctaacat cgaagcagaa atcaatgcat ttgtaaaata aagacctata
     8881 aaagatgaga ccaaccaacc tcatcttttt tcgtatcaaa tctaagcaat tttagaaact
     8941 ttatggcata ctagacttat caatgaaagc gaggtaatct catggaacaa tttttagata
     9001 acatcaaaga ccttgaagtc actacagttg tgcgtgcgca agaagctctt gataaaaaag
     9061 aaactgcaac cttctttatc ggtcgcaaaa cttgccctta ctgccgtaaa tttgcaggta
     9121 cattgtcagg tgtcgtagct gaaaccaaag ctcacattta cttcatcaat agtgaagaac
     9181 caagccaact caatgatttg caagcattcc gctcacgtta tggaatccca actgtaccag
     9241 gttttattca tattacagat ggacaaatca acgtccgttg cgactcttca atgtcagcac
     9301 aagaaatcaa agatttcgca ggattgtaaa aaatcagcta acaagtctta aaatcacaaa
     9361 aacgcataat gtaaggtgca aaaaccttga tattatgcgt tttattgttc ctaaaacagt
     9421 tacatcagca tttcattaca gaacatttta attttggaag tcaaatcaat ttttaacaat
     9481 gttttagaaa tcacagtggg ctattccata ttcaattatc cagattcaat cgctattatc
     9541 gttttcctag attacaaaaa aaactacgtt ttgctaatca aaacgtagtc tcagccacta
     9601 ttttatctta ctgtccttct tttacttctt tggttgctac atcttctcca aaccattttt
     9661 ggctgatttc ttggaacttg ccgtccttgt aaagactaga aaaagcttca tttatcttct
     9721 taaccaaggt tgtatcttcc ttacgggctc caaccgcaaa agcttctgtt tctagtccaa
     9781 ctgtaaagac attataatcg tttaaaacac cttctgcttc taaataatag tttgcataga
     9841 cacggtcaat caatagacca tcaattcgat cgtttttcaa atcaatcaag gcttcattaa
     9901 aggtttggta ttgattcgct tccttattag cgacaatatt cttcaaaagt tctggatttg
     9961 cttcaaagtc cgcataacca gatgaaccag cttgagctcc taatgtcttt ccagtcatat
    10021 cctttgcagt cgtgatacca gatgatttct tcgtaaccaa tacctgctca ttcttcatat
    10081 atgagttact gaaagccacc ttttcacggc gttcgtctgt agcggaatag ccattccaaa
    10141 tcagatcaat cgttcctttt gtcaattcag cttctttcaa atcccaatca atcggttgcc
    10201 aatttaccgt gattccgtat ttttcaaaaa cagctgtagc taaatcaata tcaaatcctg
    10261 cataagaacc atctttctga gcaaatccca ttggaacaaa agtactatca aatccaatag
    10321 taatagactt gttagactgg tactttgacc aattatctcc actagtttcg ctagaatttt
    10381 tcccacaagc tactaagaac aaagcagtca tcagactgac taatacaagc atccattttt
    10441 tcatccattt ttcctcctat ttaggttcta ctttcaataa tacatctgcg atattttcag
    10501 caaactgcaa atcatgggta accacaatct gggtcatccc aagttcccta ttttgcaaga
    10561 ttagcttctc cacttccaaa cgtaattctg gatccagggc agaagttggt tcatcgtagc
    10621 caatgatttc tgggtcaatc atcatagcac gcgccaaagc cacccgctgc ttttgcccac
    10681 cagatagtga gaaaggatag gcctctgcgt gtcctcctag tcctaactgt tccaagagtc
    10741 cactcgcctt cttctcagcc tcttcctgct tcattcccat ggtcttcaca ggcgataagg
    10801 tcaaattgtc cagaactgat aaatgaggga acagttgaaa atcttggaag acaaatccca
    10861 gtagattgcg cttctgcaat tcatccagct ctaaaggttg tccattataa aagatttgcc
    10921 ctgaatcaat ggtttcaaga cctgcaagca tacgtaaaag agttgtctta cctccaccag
    10981 aaggtccaac gatagccagg atttgctttt caggaatact tagactgaaa ttagacagga
    11041 tttgtttgtc tccaaagact ttattgatat ttcgtaattc taacatggca gcctcctatc
    11101 tataataact atacttcttc tcaacttttt tggaaataat tgtcacaatc ccaatcaaaa
    11161 tcaaataaat ggctcctgcc aagaacatag gaattagact agcatcgcgg ttagcagctg
    11221 ttcgactagc caagataaga tctgaaattc cgagagcata gaccaaagaa gtatccttga
    11281 ccaaactcat aacttcatta aagacactag gaagaacgat cttggtcact tggggcaaga
    11341 taatatagcg cactctgtca aaagggctaa acttcaagac cttggcggcc tcatactgtc
    11401 ctcttggaat agtgtcaatt cccccacgga aaatttctgc aaagtaagct gcatagttga
    11461 gaacaaaggc aataatagct gcaggaaggc ggtctaaacg aatcccaata cttgggagca
    11521 cataatagat aaaaatcagt tgcaagagta agggggttcc tcgcataacc cagatgtaaa
    11581 tgttaatcag ataatggagg ggcttccaat ggacttgcaa ggcaaaggca atcaaaacgc
    11641 ccaacggaat cgaaaatagc aagaccagtg caaagacctg tacagtcgtg ctcgcaccat
    11701 tcagtaaact cggtaatatc tcaaataaat aagacatact gcacctccta aaagtaattg
    11761 ttcctattat agcatgaata aacaagatta ccaagtattt ttgtaaaaag attctaccat
    11821 atacaaataa aaaccgtagc tatcaaaaga gataaactac agttctatat gtttaaacat
    11881 tcttacaatc tctcaaacag ttcttgcatt tgcacatcat ctggaaccag ttttaagtaa
    11941 gtgtgagcat ggacttttgc ttcttcaaaa tgtcccaatt cacgcaagag atagatatag
    12001 tgttccagaa attctggatt gtccttcaaa tctcctgtca actcttgata atgctcataa
    12061 gcagtatcca aatcgtccat ttcttgataa gaacgagcaa tcatccactt ggtcaaaaga
    12121 ttttctggct cctcactctg caagtctaga atatcctcat aacgctcctg ctccagataa
    12181 atagtggcta aacgaagcaa gatttcttct gtatcctcag cgtcttcttt tgcagtaagg
    12241 agataatttt ctgcaccact agcatcatgc aattcataag aaaattgtga agcagctagc
    12301 aagaggcgag tttcaaaggg atttttctct aatccttgct tagcgatacg cagggcttct
    12361 tgaacttgat gttccttatg taaagcctga ctgtacccat actcatagcc ttcaaagtca
    12421 ggagaaatgg tatcaagctg cttaaagtag agggtggctt tttgatattc ttcttgatca
    12481 aaataaagac tggccaactc aaaagctgtt aagtcatcgt attctaactc cagggctttt
    12541 tctaaaaact cagtagccgt ttcaaatttc cctaactgag cataggcaaa gccaattcgt
    12601 tgataggtgg aaatgcccgt ttgctcataa atcgagcgat tatctaactg ggcataggct
    12661 tgaatagccg cttggtaatt ttccaactca ctatccaact ctgccaaacc caatatcaag
    12721 agagaatcct ctgagtaggt caaggcctcc aataatttct cacgtgccac atctgtcaaa
    12781 ccttccagct ggtaaaggtc tgccttcaga gccaaagacg agacatacca gtcactgtca
    12841 gcttggattt cctcaagata ggtaaaggct tcttctattt gaccatcctc gctagcaatt
    12901 gcagctagat taagatgaac ctctggaaaa tcctctacaa ttttcaggta aatttccttg
    12961 gcctgaggat agaaaccaat cccttcaaga taagttgcca actcatacag aagatcactt
    13021 gaatcatttt ctaaagcttt ggcgaaataa tgctcagcct tagttaaatc ttgctcctcc
    13081 aaagcctgta acatctgttg actattgttc actcttgact tcctcaccct ctaattcata
    13141 tagaccactg actaccttat accattcgaa aatggctgaa atgacaacct tagcagaggc
    13201 ataaaccgga ataccaagta aaactcccca gataccaaac atagatcctg aagttaacaa
    13261 aacaaagaga acattaatag gatggatgtt taattgactt cccaaaatca atggagagac
    13321 aaaacggcct tcaatagttt gttctacgat aaagacaatc actactttca aaagcatgac
    13381 tggaccagca atcaaaccca atactagagc aggaagcatg gctagaaagc taccaagata
    13441 agggaccaga tttaaaatac cagcagtaac ccccagcgta accgcatagc gtagaccaat
    13501 aatcttgaag aagatgataa acattactgc tacaataata gccactgtca cttgccctcg
    13561 aacatagttg gacaactgtt gattcacatc tgataaaact tgtccaacag gttccttcaa
    13621 ttttcttgga atgaattggg tcaaatagtt acgcaagcct ttcccatcac gcaagagata
    13681 aaagagcatg aaaggaacga taatcaaggc aacaatcact tgagaagccc cgctaataaa
    13741 ggcactcacc cagttgactg cctgagatga aaccttactt gccaaaactg tagcctggct
    13801 agaaaaattg gtcaaaactt gctctaattg aggtctgaaa tcatctggca ggtgctgggc
    13861 taccaatcca ttaacaatcc tatctatatc ttctaagtaa acaggaacgt ttcttgcaaa
    13921 ggtcaaaacc tgacgttgca gatttggaat ggcgactgcc aagccccaaa tgataaagag
    13981 agcgatgata acaaagacaa tagtgatagc tataacacga ttaaccttat gcttctccat
    14041 ccaatcaaca ataggattca acaaataata taacaaacca gacaaaatga ctggcaacat
    14101 cacaactgct aaaaagtcta aaacaggtga aaatagaaaa ctaatcttac ttaaaataaa
    14161 aagattcagt cccaataata aggttactaa aaataccgta attgccttgt tatctaaaaa
    14221 ccacttgaaa aaccaagata ggctaaaatg tttctctttt tgctccataa atacttcctt
    14281 tctattgttc tctcattata ccatttttga aacaaaataa ctctttttta aagtgcttac
    14341 atctaattgg atgtctttaa tttgcttgtt tttgtgatat aatagtctta tctttacaca
    14401 gaggtatagt aatgaaagaa actgtttatt ttggaactta tacacgtcgt acttctcaag
    14461 ggatttacaa ggcagacttt gatacagaaa ctggtcagct ttcaaatcta gaactttttg
    14521 cagctgagcc aagtccaacc taccttgcct ttgaccagca ccaacattta tacactgttg
    14581 gtagccaaga cgataagggg ggaattgcag cctatcaaac tgacgggact gtgttaaatc
    14641 atgttgttga agaaggagct ccccactgtt atgttgctgt cgatgaaaag cgtgatttgg
    14701 tttacgcagc caactatcac aagggacaag tccttgttta taaacgccag gaagatggta
    14761 gtcttctact tagtgatatg gatcaacaca gtggccaagg tccacatgaa aatcaagctt
    14821 ccccccatgt tcactataca gatttaacac ctgaccacta tctagtgacc tgcgacttgg
    14881 gaactgacca agtcatcacc tatgacctcg atcaagaagg aaaattatct aagctctata
    14941 cctatcacag caagccagga gcaggctcac gccatatcat tttccataac cactataaaa
    15001 tcgcttatct catttgtgaa ctcaatagta ctatcgaagt tttaatctac gatggcgttg
    15061 gcgaatttgt acgtatgcag gttatttcaa ctttaccaga agcttacgaa ggctttaatg
    15121 gaaccgctgc tattcatctc tctaaagacg gtaaatacct ctacgcttct aaccgtggcc
    15181 atgattctat cgcagtatat accatccttg cggacggtag cttagagtta ttagaaatcg
    15241 ttccaacgca tggtcagact ccacgtgatt ttgatttgac ccccgaccaa aaatttctca
    15301 ttgttgtcca tcaagactct gacaatgcaa ctgtctttaa acgtaattgt gacaatggtc
    15361 gtctagcaga actctccaac gacttccatg ttcccgaagc agtctgcatc cgttttgctc
    15421 cttaaaacac cataaaaaat gaacttggta ttttcaagtt cattttttct tatagtctat
    15481 ttccgacatt aatacggtta atggcacgtt gcaaagcaat cttagcacga cgttcttggt
    15541 caatcaaatg tttgtcttgt gcttcttcaa ttgcacgttc tgcacgaagt ttggcacgtt
    15601 ctgcacgact gatatcgata tcacgagcac gttctgcaga gtcagcgacg attgtgatca
    15661 tatcattggc aatttcaata acgccaccgt ttactgcaat ccagttcacg tgatctttat
    15721 catcgatacg ttttaccttt acttcatcaa ctgctaaaac cgcaatcata ttttcatgtc
    15781 gtggcaagat ccccatctca ccatccagag ttcgaaccga tacatagctg gcatggtgat
    15841 catagacgag accatctggt gtcacgatct ggacagttaa ctgagccata ggtcacctct
    15901 taaaatccca ttttttcagc ttttgcaatc acatcttcga tagaacctac accacggaag
    15961 gcatcttctg gcaagtggtc gtatttacca tcaaggattt ccttaaagcc acgtacagtt
    16021 tcagcaactg gaacataaga acctggctga ccagtaaatt gttccgcaac gttgaagttt
    16081 tgtgacaaga agaactggat acgacgggcg cgagcaacca aggtcttttc ttcatcagaa
    16141 agttcatcca taccaaggat agcaatgata tcttgcaatt catggtaacg ttgaaggaca
    16201 cgttttactt cagcagcaac tgcatagtgc tcttctccaa cgatttcagg tgccaaggca
    16261 cgtgagcttg aagcaagtgg gtcaacggct gggtagatac ccaattgtac caacttacgt
    16321 tccaagtttg ttgttgaatc caagtgagcg aaggctgttg ctggcgctgg gtcagtatag
    16381 tcatccgctg gcacatagat agcctggata gaggttacag aacccttctt ggttgatgtg
    16441 atacgttctt gcaattgacc catttccgta gcaagtgttg gttggtaacc aacggctgat
    16501 ggcatacgac ccaaaagggc agatacttct gaaccagcct gagtgaaacg gaagatatta
    16561 tcgataaaga gaagcacgtc ttggccttcc acatcacgga agtattcagc gattgtcaaa
    16621 ccagtaaggg caacacgcat acgtgctcct ggtggctcat tcatctgacc aaagaccatg
    16681 gctgttttct cgataacgcc tgattctttc atttcccagt aaaggtcatt cccctcacga
    16741 gtacgttccc caacaccagt aaatactgaa ataccaccgt gctcttgggc aatgttgtga
    16801 atcaattctt ggattaagac ggttttacca actccggcac caccgaaaag tccaacttta
    16861 ccacctttaa ggtaaggggc aagaaggtca ataaccttga tccctgtttc aaggatttca
    16921 gaagaggtag acaactcatc aaaagttgga gcttttttat gaattggctg acgctctgcg
    16981 tcttctgtaa aaggagcttc caaatcaatg gtatctccca aaacgttgaa gacacgtccc
    17041 aaagtttctt tacctactgg tacagagatt ggacgacccg tgtccaatac ttccattcca
    17101 cgagtcaacc catctgttga ttccatggcg atagtacgaa ccataccatc tcctaactcc
    17161 aaggctactt caaggacgat ttttgttttt ctttcgtcat ttttgtagac gacaagtgca
    17221 ttgttaatct caggaagttt ttcccctgct gcaaacaaaa cgtctacaac gggaccgata
    17281 acctgagcaa ttttacctga actcatctcc ttctcctatt ctatataaga tactgtcggt
    17341 tcctagctca actaggtcct aagttcattt tcatacgagc tggactagag cctattctaa
    17401 ggcactagca cctgctacga tttctgtaat ttcttgtgta atcgccgcct gtctggcacg
    17461 gttatactga attgtcaaat cattgatgac tttcttagca ttatctgtcg ctgtttgcat
    17521 ggctgtcata cccgcagcat tctcagctgt cttggcatcg ataatggcac cgtaaatcat
    17581 actttctgca aactgaggca acaactgctc cagaatttct tctcggctgg tttccaattc
    17641 aaaagtcaag ctgtactctt catccgcttc atttggatcc aagtcaacaa tcggaagcat
    17701 ttgttccaca cgcatttgac tggttagcgt attgacatgg tggttgtagc aaacataaag
    17761 ctcatcaaag agttcatttt ggtacatttc aacagttttt gaaataatct tacgaacttg
    17821 atcaaagcta ggttggtctg ccaagccacg taattcataa agtggttgaa taccgcgagc
    17881 cttaaagaaa tcagctccca tcccaccgat acagatcatt tcaaaacctt taccgtctgg
    17941 gtggtattct tctttcaact ccataacagc tttcaaaata gaggaattat aacctccaac
    18001 caaaccgcgg tctgaagtga taacgatata gcctgtcttc ttcacagaac ggctaatcaa
    18061 catcggatta gttgaagcac cagctccatt accatgaagg atatctgtca aaagtttacg
    18121 cactttctga gcgtaaactt ggaagttgcg agcagcttct tcagagcgac ctaacttagc
    18181 agccgatacc atttgcatgg cattagtgat ttgactcgta ttttttgttg aggcgatttt
    18241 tgttttaata tcatttagag acactgccat ctgacacctc tattcttatt ggaagctaga
    18301 ttgattgaga aactctgtaa tcgcagcatc caagactgct tcttctggca agtcttttgt
    18361 atcacgaatg gtttccaaaa tctctggatg ttgagcatca aagaaggcat ggaactcttc
    18421 ctcgaaacga acaatatcat ctactggaac agtatccaag aaaccatgtg tcaaagcata
    18481 aagaatggtt acttgtttct caacaggtaa tggtttgtga acaggttgtt tcaagacctc
    18541 aacggtacga cgtccacggt tcaacttagc ctgtgttgct gcgtccaagt cagaaccaaa
    18601 cttagtaaag gcttccaact cacggtatga agcaaggtcg atacgaagtg taccagcaac
    18661 cttcttcatg gctttgattt gtgcagaacc acctacacga gatacagatg aacccgcatc
    18721 gatggctgga cgaatacctg cattgaagag gccatcgcca aggaagattt gtccatcagt
    18781 gatagaaatc acgttggttg cgatataggc tgagatatct cctgcttgtg tctcgataaa
    18841 tggtagggct gtaattgatc caccaccaag ttcatcagaa actttagctg agcgctcaag
    18901 caaacggctg tggagataga aaacatcccc tgggaaggct tcacgacctg gaggacgacg
    18961 aagcaagagc gatagttcac gataagctac cgattgtttt gatagatcat cgtatacaat
    19021 caaaacatgc ttaccttgat acataaattc ttccgccata gcaaccccag tataaggagc
    19081 taggaagagc aatggagatg gttgtgaagc agaggctgtc acaacgattg tgtagtccaa
    19141 ggcaccgtac tgacgaagtg tttctacttg cgtacgaact gttgattctt tttgtccaat
    19201 cgcgacgtag atacagatca tatcttgatc tttttggttc aagattgtat caatcgcaat
    19261 ggttgttttc cctgtctgac ggtcaccgat aatcaactca cgttgaccac gaccaatcgg
    19321 tacaagggcg tcaatagctt tcaaaccagt ttgcaatggt tctgaaacag acttacgttg
    19381 cataacacca ggagctggtg cttctactgg acgagtttta tcagtgtgga tttctccaag
    19441 accgtcaact ggacgaccaa gcggatccac aacacgacca atcagacttt cacctacagg
    19501 gacttccatg attttccctg tacggcggat tgtatcgcct tcacggatat ctgtaaagtc
    19561 acctaggatg ataataccaa cgtctgttga ctccaagttt tgagccatac cataagagcc
    19621 gttttcaaaa ttcaacaact ctccactcat gacattttca aggccatgag cacgcgcgat
    19681 accgtccccg atataggtta caacacctgt ttcagtcaca tcaaaattgg gtttgaaatt
    19741 ttcaatttgt tgcttaatta aagcgctgat ttcttgtgcg ttaattgcca aaagaacacc
    19801 actttctatt tcaaattttc tttaacaact ttaagttgtt gtttaatact cacatcaatt
    19861 gtcttgtgat tggcaaaaat gacaaaacca ccaatgagac tttcatcgat ttgttctttt
    19921 acactcctta ctttcagaga catttttttc tcaatcaaag ggagcaaacg agtcttctgt
    19981 tcatcagtta gaggatgagc agacgtaatc gtcacttcaa atcgatttgt ttctttttca
    20041 agtcggttca agcaatctac aagcacatca taaaaaagat ttgctctgtg attgtaggcc
    20101 agaacctgga taaagttttg taataaaggc gacacagaat cttggaaaaa agcaattgtt
    20161 ttttccttat ccgactcgtc tactgccact tgttttaaaa aagaaggcag acctgttttt
    20221 tcaacaactt gcttgatttg agtcaagtct gaaaagatac ggtcttcttc tcctttttca
    20281 agtaccaatt ggacaaaagg catgctgtat ttttcaatta cctttactgt tttcttgtcc
    20341 attaagcttc tcctagctga tcgatatact gatcaatgag tgctttatgg gcatgactgt
    20401 caaggttttg tgagatgatt ttaccagcta agctgatggt caaatctgcg acctcaccct
    20461 taacactctg taaagcttct actttatttt gagcaatttc ttgattggct ttttctttta
    20521 agtgtcctgc ttctagttta gcatctgcta agatattagc cttactttgc tcagctgttt
    20581 cctttgcatt ttcaatgatt gtcttagctt ctttacggct accagccaat tcatcttcgc
    20641 gtttttgagc caatacttct gctttttgac gggcttcttc agctctgtca atatctgaag
    20701 caattttttc agctctttct tcgaaaatgc ctgtaatatt agaccatgca aattttttaa
    20761 tcaagactag caaaagaata aaagagccag tgattaaaat aaaattacca attaattcac
    20821 ctactgttac gtgcatcagt tactcctttc tattcctctt caatattaat cttattccct
    20881 aaatacacag atgtcaaaag agtaaaaaca taagcttgga tgcaggaaat aaagacagaa
    20941 aatgcagtcc aagccaaatt agctccaaag gctactggat accaataaat agcttggtgg
    21001 gaaagaagaa gtaacaaact cgtcatgacc tctcctgcaa agatattccc aaaaatccgc
    21061 aaagccaaag ataagaagtt tgtaaattct tccaagatat tcatcggtat gacaaaaaca
    21121 ggactcataa aagattttat acttttttta aatccacgac gacgaacgct ttctatatgt
    21181 gtcaacaaaa tgacaagaaa agataaggtt aagtcatact gtaaattagc ggttggcgaa
    21241 ctccaccagt tagtcccatc gatcgtttga agctttgtca ttaagccaag gttattggca
    21301 atcaccatga aaaggaataa acaaaggaaa aagagtgagt aatctttcat gtagcgcgaa
    21361 ccaatgttag gttctgtaaa tccaataaca aagtcataga catactcaag tacattttgc
    21421 tttcctttgg gtttcaaggt catattgcgg cttgcccaat aaataaagac aaaaataact
    21481 cccacaatca acaaagtcat ggctaacata gtcagattga agataacagg accaatagag
    21541 atgattggat taatactttc ttccatctaa aatcctccct tttcttctcg ttccatgtat
    21601 ttatttgata ataaatgaga agacaagagt tacaaagaaa gttccttcaa taaaggcaac
    21661 acctaaaaac atcaaactac gaaactcaga aagcatatct ggttggcgtg ctactgattt
    21721 aaacagtcca ttcatcaata aaccttcacc gacagatacg cccatacagg caatacataa
    21781 gcctaaaaat gttaaattca taacaaattc tcctttaaaa taaaatttac ttacttagtg
    21841 tagtcctaaa atttaaattt gtcaactttt aaccgctatt ggaagcgttt aataaaaatt
    21901 atttccattt tgctaaattt caagttcaag ttagccatcc agaagtcttc tctgggtgac
    21961 ttgtagtcca ag
//
LOCUS       DAVBGY010000033        21572 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVBGY010000033 DAVBGY010000000
VERSION     DAVBGY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21572
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.033"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..128
                     /locus_tag="VQ620_001261"
     CDS             <1..128
                     /locus_tag="VQ620_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2763882.1"
                     /translation="FRSLENLEQAIVDYIDYYNNKRIKVKLKELSPVQYRTKSFG"
     gene            complement(176..2719)
                     /locus_tag="VQ620_001262"
     CDS             complement(176..2719)
                     /locus_tag="VQ620_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HET2763883.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQKKVD
                     ILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTKTFT
                     EAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQAQT
                     GFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(2781..4403)
                     /locus_tag="VQ620_001263"
     CDS             complement(2781..4403)
                     /locus_tag="VQ620_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2763884.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            4610..5635
                     /gene="trpS"
                     /locus_tag="VQ620_001264"
     CDS             4610..5635
                     /gene="trpS"
                     /locus_tag="VQ620_001264"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET2763885.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            5788..7266
                     /gene="guaB"
                     /locus_tag="VQ620_001265"
     CDS             5788..7266
                     /gene="guaB"
                     /locus_tag="VQ620_001265"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET2763886.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(7317..8414)
                     /gene="recF"
                     /locus_tag="VQ620_001266"
     CDS             complement(7317..8414)
                     /gene="recF"
                     /locus_tag="VQ620_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET2763887.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(8417..8785)
                     /gene="yaaA"
                     /locus_tag="VQ620_001267"
     CDS             complement(8417..8785)
                     /gene="yaaA"
                     /locus_tag="VQ620_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HET2763888.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKIDIPDMNIDILLTQSTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            8944..10194
                     /locus_tag="VQ620_001268"
     CDS             8944..10194
                     /locus_tag="VQ620_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET2763889.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSYLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHCPSKYGDEKHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSQLDLFSGFLRMYAGIDRENRNQARKMMNNQLLDL
                     KKGYFTELELEQTKEMIRRSLLLSQDNQSSLIERAYQNALLGKSSADFKSWIAKLEQV
                     DKDAICRAANNVKLQAIYFMEGIE"
     gene            10191..11474
                     /locus_tag="VQ620_001269"
     CDS             10191..11474
                     /locus_tag="VQ620_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET2763890.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTLVTEVDGDVKQYPAGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELITSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKLTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            11516..12337
                     /locus_tag="VQ620_001270"
     CDS             11516..12337
                     /locus_tag="VQ620_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2763891.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            12348..12893
                     /gene="pgsA"
                     /locus_tag="VQ620_001271"
     CDS             12348..12893
                     /gene="pgsA"
                     /locus_tag="VQ620_001271"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET2763892.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            12890..13717
                     /locus_tag="VQ620_001272"
     CDS             12890..13717
                     /locus_tag="VQ620_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET2763893.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            13702..14541
                     /locus_tag="VQ620_001273"
     CDS             13702..14541
                     /locus_tag="VQ620_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET2763894.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPNQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            14534..15328
                     /locus_tag="VQ620_001274"
     CDS             14534..15328
                     /locus_tag="VQ620_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2763895.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            15387..16205
                     /gene="mreC"
                     /locus_tag="VQ620_001275"
     CDS             15387..16205
                     /gene="mreC"
                     /locus_tag="VQ620_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET2763896.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKVSQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            16205..16699
                     /gene="mreD"
                     /locus_tag="VQ620_001276"
     CDS             16205..16699
                     /gene="mreD"
                     /locus_tag="VQ620_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET2763897.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            16793..17971
                     /locus_tag="VQ620_001277"
     CDS             16793..17971
                     /locus_tag="VQ620_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET2763898.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            18195..18974
                     /gene="rpsB"
                     /locus_tag="VQ620_001278"
     CDS             18195..18974
                     /gene="rpsB"
                     /locus_tag="VQ620_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET2763899.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            19053..20093
                     /locus_tag="VQ620_001279"
     CDS             19053..20093
                     /locus_tag="VQ620_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HET2763900.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            20340..20681
                     /gene="tnpB"
                     /locus_tag="VQ620_001280"
                     /pseudo
     CDS             20340..20681
                     /gene="tnpB"
                     /locus_tag="VQ620_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(20817..21104)
                     /locus_tag="VQ620_001281"
                     /pseudo
     CDS             complement(20817..21104)
                     /locus_tag="VQ620_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            21095..21493
                     /locus_tag="VQ620_001282"
     CDS             21095..21493
                     /locus_tag="VQ620_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2763901.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPIIRKEISNWHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
BASE COUNT         6853 a         3489 c         4425 g         6805 t
ORIGIN      
        1 gctttagatc tttagaaaac cttgaacaag ctattgtgga ctacattgat tactacaaca
       61 acaaacgaat taaggtaaaa ctaaaagaac ttagccctgt gcaatacaga actaaatcct
      121 tcggataaat taattgtcta actttttggg gtcagtacat taactggtcg attttttatg
      181 ccttagatga ctttcgtctg tgattataga ttccaaatag taagagagaa gtaaaggaac
      241 agattgctcc agtaataaaa ccattgggaa tgaaggaaag tgtaatagtt cctttcccct
      301 tgggaatgtc aactttcata aatccagttt gagcttgttt aatttctatt ttcttaccat
      361 cttggtaggc agaccaacct ttgtcataag gaatggtgaa gaaaatagat gtatcttgtt
      421 ggacatcata tgtagcaaaa accttgtttt tagaagttga tactgtgaca ggttgttctt
      481 taattttttg aattgcctcg gtgaaagttt tggtatctaa acgatagaag gtaggagatt
      541 caaatgatac ttgtgaattt ccagggaaac taacattgat attgaaagtt tttttctctt
      601 tagtatatcc tagattaaag aaggagaaga cattatcagt tgtaaaagtc tttttttcac
      661 catttacaag gatgtcaacc ttcttttgtt tatcgttaga aaagtgaagg tttatgaaag
      721 agagataaac ttggctgttt tctggaactt caatttgata ctggattgct gcatcttcat
      781 ttgaagaact tgtgacacta atcaaatcat tagtattttc tgttttttca taaggtattg
      841 gagaaaaata atcaaaattg acgttagcaa gttgatttaa aaatgaggcc tgattatcca
      901 aggtatgttc attgaacttg acatcattgt aaacagattg actagcaact gcaatcggaa
      961 gagagtattg attttcatat agggtaagat tatctttttg atagatatct ttaaagccat
     1021 acttatcaat aggactgtct gagatattgt actggatacc aaataaacta tcagccaaaa
     1081 tactattatt tgcatatcgg agattgagat tagtcccaga ggatttaaaa ccaagtttat
     1141 ctaaagtaga gcttgatgaa cgatttcgaa cagatgaaaa ttgagagatt ccattgtagt
     1201 tgaatttcat actgtcattt cctgtctgag tttgtagttt ttcagtacga gtaaattgat
     1261 ttccaatata tgttgagaaa gattccatag ctgggatatc tcgactataa gcacttcgag
     1321 aagcaaatcc ccattcctta gcaattccgt ccatttgaga tgaagcattt aaactcattt
     1381 caaccagtat aaataaagag attagaatgg caaatagatt cacagatata aactttttga
     1441 taactgcaag gagtaaaaga gaatagacaa ccaaaaattc aagagtaagc agaatattca
     1501 aatctgttaa aaaagaataa tgcgatttta gatagatggt agctaaaaat cctgctacta
     1561 caagaaaaag cgaaactaaa aaattccaga ctttaagttc tttcagacgc tttaagactt
     1621 ctgctgctgt gtaaattaac aaggtagaga aaatccaagc atagcgatgt aaaaacatgt
     1681 ttggagtatg catgccttgc caaaataagt caagagcttc tatgtaaaag cttgcaatta
     1741 gaaatgcaaa gaatattaca tatatgagtt tcacgtgaaa cttaatagat ttcagcgtaa
     1801 aaaataaaat ggtcaaaata aagggaaata gtccaacaaa aatcattggg atggccccat
     1861 actttgttgt gtcaaaggaa ccaatgaatt gcttagcaaa gagatcaaga taccagctac
     1921 tttcagtttg aaactttgta acttcagtca atttttcccc atgtgtctgt aaatcaaata
     1981 gagtgggaag agtcataatc aaactagcca taccagctaa aaaggagata actatgaaat
     2041 caagaacaga tgattttcga gtcttaaagt cccacgaaat ttgacagaga taccagaaaa
     2101 taagaaacaa tactgtcata tatccaaaat aataattttg aataaataag attgacagac
     2161 ttgtaaagta caataggagt ttcttttcag ttatcagtag atgtaaacca gttataatta
     2221 aaggaatcaa gataaaaaca tctagccagg tttttatctc taattgactg acagtgaaac
     2281 tcatcagagc ataggaagta gataaggcta gttttaaagt ctgagggata gattgaaaca
     2341 atttattcaa actaaaaaag gttgacagac caatcaatcc aaattttaag agagtggtca
     2401 gatagacagc atctggcata ttcgttagat caaaaaagta aactagaggc gcgagaaaac
     2461 tacccaagta ataactagat agggcataga agtttagccc tagaccactt gtaaaggtgt
     2521 aaaacagact actatttcca tgtaggatat ttcgtaaggc tacatcaaaa ataacgtatt
     2581 gatgaaagcc atctcctaat agaggagagt tgtcgctatt ccagtagata ccttgagata
     2641 gatatactcc agacataatc actacaggaa tgatgaaaga aataaaatag gttcgatatg
     2701 tttttaaaaa tgatttcatg ttacctcgta gaatgataga aaactcagtt ggttaaccca
     2761 actgagtttt gaagttttat ttagtctttc caaagttctt taacttttgc ttgtacttct
     2821 gcattttcta ggaattcatc gtaggtttca tcgatacggt caatgacgcc atttttagat
     2881 aagacaatga tatggttagc caaagtttga ataaactcgt ggtcatggct ggcaaagatg
     2941 attgattctt taaagttttt cagtccatca ttcaagcttg agatagattc caagtccaag
     3001 tgatttgttg gatcatcaag tacaaggaca tttgatttta agagcatgag ttttgaaagc
     3061 atgacacgaa ctttttctcc ccctgacaag acatttacag gtttgttaac ttcatctcca
     3121 gagaagagca tacggccgag gaaaccacgt aggaaagtat tgtcatcttc ttctttactt
     3181 gcgaattgac gcaaccagtc aagaattgat tctcctcctg caaaatcagc tgagttatct
     3241 tttggtaggt aagattgact agttgtaact ccccacttga cagttccttc atagtcaata
     3301 tctcccatga ttgcacgaat taatgcagtc gtttgaatat cattttgtcc aataagtgct
     3361 gtcttatcat ctggacgcaa gatgaaacta atattatcca agatagtttc accatcaatc
     3421 tttacagtta gattttctac tgtcaagaga tcattaccaa tctcacgttc cgctttaaag
     3481 ttgataaatg gatatttacg actagatggt acaatctctt ctagctcaat cttatcaagc
     3541 attttcttac gtgatgttgc ctgccttgac ttagaagcat tggcagagaa acgagcaacg
     3601 aattcttgca attgtttaat tttttcttct gctttagcat tacggtctgc tagcaattta
     3661 gcagcaagct cagaagattc cttccagaag tcgtagtttc cgacatagag tttgattttt
     3721 ccaaagtcaa ggtcggccat gtgagtacaa actttgttta agaagtgacg gtcgtgggat
     3781 actacgataa ctgtgttatc aaagtcaatc aagaagtctt ctaaccaagt aatcgattgg
     3841 atatccaaac cgttagtagg ctcgtccaag agaagaacat ctggtttacc aaaaagtgct
     3901 ttggcgagga gaacctttac tttttcaccg ttggccaatt cgctcatgtt ttggtagtgt
     3961 aattcttctg gaatgtttag gttttgaagt agttgagagg cttcactctc tgcttcccaa
     4021 cctccaagtt cggcaaactc tccttcgagt tcggcagcac gaaccccgtc ctcgtctgag
     4081 aaatcttcct tcatgtagat agcatctttc tctttcatga tgctataaag tttttcattt
     4141 cccatgataa cgacatcaat ggcacgttca tcttcgtagt caaagtgatt ttgacgaaga
     4201 acagagagac gttcatctgg accaagagag atgtgaccag tagtaggttc gatatctcca
     4261 gctaaaattt ttaaaaaggt tgattttccg gcaccattag caccgattaa tccgtaagta
     4321 tttccttctg taaatttgat attgacatca tcaaaaagtt tgcgatcact aaaacgtagt
     4381 gaaacatcag atactgtaag caatgttttt ctcctatatg tgtaatatat ttattctact
     4441 agaaaataca gaaatattca aatttttatt tgtcaatttt gtgtaaatta tatttacagt
     4501 atcctttaca caaatctgta aaaagcaagg ctgatttatt ttgataaatt acggttattt
     4561 cattaaaaaa aatgctataa ttgaaaggac tatatcgaag gagaacaaaa tgactaaacc
     4621 cattatttta acaggagacc gtccaacagg aaaattgcat attggacatt atgttggaag
     4681 tctcaaaaat cgagtattat tacaggaaga ggataagtat gatatgtttg tgttcttggc
     4741 tgaccaacaa gccttgacag atcatgccaa agatcctcaa accattgtag agtctatcgg
     4801 aaatgtggct ttggattatc ttgcagttgg attggatcca aataagtcaa ctatttttat
     4861 tcaaagccag attccagagt tggctgagtt gtctatgtat tatatgaatc tagtttcgtt
     4921 agcacgtttg gagcgaaatc caacagtcaa gacagagatt tctcagaaag gatttggaga
     4981 aagcattccg acaggattct tggtctatcc aatcgctcaa gcagctgata tcacagcttt
     5041 caaggctaat tatgttcctg ttgggacaga tcagaaacca atgattgagc aaactcgtga
     5101 aattgttcgt tcttttaaca atgcatataa ctgtgatgtc ttggtagagc cggaaggtat
     5161 ttatccagaa aatgagagag cagggcgttt gcctggttta gatggaaatg ctaaaatgtc
     5221 taaatcacta aataatggta tttatttagc tgatgatgcg gatactttgc gtaaaaaagt
     5281 aatgagtatg tatacagatc cagatcatat ccgcgttgag gatccaggta agattgaggg
     5341 aaatatggtt ttccattatc tagatgtttt tggtcgtcca gaagatgctc aagaaattgc
     5401 tgatatgaaa gaacgttatc aacgaggtgg tcttggtgat gtgaagacca agcgttatct
     5461 acttgaaata ttagaacgtg aactgggtcc tattcgtgag cgccgtattg aatttgctaa
     5521 ggatatggga gaagtttata atatgattca aaaaggtagt gaaagagcgc gtgaagttgc
     5581 gggtcaaacc ctatctgagg taaaaggggc aatgggactt cattacttta actaagttta
     5641 ttttacaaga aactatcatt tctatctcga agaaaagata gtttttttat ttacccctgt
     5701 aacatttgac aaatttttat agaatggtat gatagataca atataaaaag agtcaagctc
     5761 aaaaagaaag aaaagaggaa acttcgaatg tctaattggg acactaaatt tttgaaaaaa
     5821 ggttttacct ttgatgatgt attgcttatt ccagctgaaa gtcatgtgtt gcctaacgat
     5881 gcagatttaa caactaaatt ggcagataat ttgactttaa atatcccaat tattaccgct
     5941 gccatggaca cagttacaga gagtcaaatg gccattgcta ttgctcgtgc aggcggtctc
     6001 ggagttatcc ataaaaacat gtcaattgct caacaagcag acgaggttcg taaggtaaaa
     6061 cgttctgaaa atggagttat tattgatccg ttcttcttga cgcctgaaca tacaattgct
     6121 gaagcagatg agcttatggg tcgttaccgc atcagtggtg ttccagttgt tgaaacactt
     6181 gaaaatcgta aattggttgg tattttgaca aaccgagatc ttcgttttat ttcagattat
     6241 aatcaaccaa tttcaaacca tatgactagt gaaaatcttg ttactgctcc tgtgggtacg
     6301 gatcttgcaa cggctgagag tattcttcaa gagcatcgta ttgaaaaact tccgttggtc
     6361 gatgaagaag gcagtctttc tggtttgatc actatcaaag atattgaaaa agttattgag
     6421 tttccaaatg cggctaaaga tgagtttggt cgtcttctag ttgcaggtgc agtaggtgtt
     6481 acttcagata catttgaacg tgcagaggct ctttttgagg caggagcgga tgcgattgtt
     6541 attgatactg cacatggtca ttctgcaggt gtcttgcgta aaattgccga gattcgtgct
     6601 catttcccag atcggacttt gattgctgga aatattgcta ctgctgaagg tgcacgtgcc
     6661 ctttatgaag cgggtgtaga cgttgttaag gttggtattg gaccaggttc tatctgtact
     6721 actcgtgtga ttgctggtgt tggtgttccg caagtaacag ctatctacga tgctgcagct
     6781 gttgcgcgcg aatatggtaa aacgattatt gctgacggtg ggatcaagta ttctggagat
     6841 attgtaaaag cacttgctgc aggtggaaat gctgttatgc ttggatctat gtttgctgga
     6901 actgatgaag ctccaggcga aactgaaatc ttccaaggac gtaaattcaa gacttaccgt
     6961 ggtatgggat caattgctgc tatgaagaaa ggttcaagcg accgttattt ccaaggttct
     7021 gtcaatgaag caaacaagct tgttccagaa ggaattgaag gtcgtgttgc ttataaagga
     7081 gcggcagctg atattgtttt ccaaatgatt ggtggtattc gctctggtat gggttactgt
     7141 ggtgcagcta accttaaaga actacacgat aatgctcaat ttattgaaat gtctggtgct
     7201 ggtttgaaag aaagccatcc tcatgatgtg caaattacta atgaggcacc aaattattct
     7261 atgtaaaaaa caatgaaaag aactccagtg aaaacaggag ttcttttaca atgttgtcaa
     7321 tttccattta cagcagcttt accatcctga atagtgaaga tacttagatt ttctggcaga
     7381 ttttgaagat ggtctaagct tgttgttgtg ataaaggttt ggattgattg agaaatcgtt
     7441 tctaataatt ttaactgtct agtgttgtca agttcactca tcacatcgtc aagcagtaat
     7501 ataggagatt ctgtggtaat gctttccatt aattcgattt ctgctaattt tatcgagagg
     7561 acgagactac gatgttgacc ttggcttccg aaactagcat ccatcccatt tatataaaaa
     7621 gaaatgtcat ctcgatgagg accgacacca gtattctttt taaataaatc tctggatcta
     7681 cttttttcta aagcaatttt gaaagattcg gataagtttt gtttgtcagt tatattgaca
     7741 gaagattgat aggatattga caactcttcg atctgattag agagttcaaa atgtttctta
     7801 cgcccaaatg attctagttt ttttatgaaa tctaagcggt gattcattac acgacatcca
     7861 taatcaacta gctgatcatc taacacagaa aggaatgttt catctatttt ttgagctgat
     7921 tttaggtaag tgtttctttg ctttaggatg tggttataat tggttaagtc agataaatag
     7981 attggcttaa tttgcccaag ttccatatca atgaattttc gtcgaatcga aggtgctcct
     8041 ttaattagtt gtaaatcttc aggagcaaat aagacaacat tcatgtgtcc tacataatct
     8101 gaaaggcgtg cctgttttaa gtgattaact tttgttacac ggcctttttg tgttagttcg
     8161 atttctaggg gaatggatcc agtttttttc tgaacgagac ctgaaagatg aagttgttcc
     8221 tcatcaaaat gaatgagatt tttatctgtt cgagttcgat gactacgcgt taaggctaaa
     8281 aaatagatag cctctaacat gtttgttttt ccttgtgcat tacgtcccaa aaagacattc
     8341 aatttaggat taaagtctat tttcgtttct ttgtaattac gaaaagtctt gagagataga
     8401 tgttgtagcc acatgattat ctaccaggga atcgtggagc ttgttggctt ttaggtgatg
     8461 aagcaggttt cgatttgtct tttttaactc ccttattcat ctttttgaca agtttagcga
     8521 tccgttcttt ttcaacttta tcagcttggt attcatcttg ttcttcagaa gtagattgtg
     8581 tcaacaagat gtcaatgttc atgtcaggga tgtcaatttt atcgccaata cgaagttttt
     8641 taccacgacg actctctaat tccctattaa agtaaacaga atgttcagag agaaatgatt
     8701 taatagctcc tccgctatgt gtaattccaa gttctttgag tagtgcttgg agggtaataa
     8761 attcttcaaa taatttgtat tccataattc acctcaatct ttggtattat accatatttt
     8821 cctaaaaata gtgagagata cagcgagaaa tgtaagcgat ttttatttca aaagtgttac
     8881 agggaacaag aaaatttcag gttttcgtga tataatagaa gtctgtatat aaggagataa
     8941 atcatggagt tagtgcatgg aatttcaaca cattttatcc aatcaaaaaa gtttaaaaca
     9001 aacaaaatta ccgtgcgttt taccgctcca ttatcccttg atacgattgc aggtcacatg
     9061 ttgagtgcaa gtatgctaga gactgctaat cagatgtacc ccacttctca agatttgagg
     9121 agacacttgg ccagtctata cggtacagat atgtcaacca attgtttcag aagagggcaa
     9181 agccacatta tagaattgac atttacctat gttcgtgatg agtttttaag taggaaaaac
     9241 gtgctaactt ctcagatttt ggaacttgta aaagaaactc ttttttcacc cgtagtagtt
     9301 gataatgggt ttgatccggc cttatttgaa attgagaaaa aacaattgct agcaagttta
     9361 gcagctgata tggatgattc tttttatttt gcacataaag aattggataa attgtttttt
     9421 catgatgaac gtcttcaatt ggaatatagt tatttacgaa atcgtatttt agctgaaact
     9481 ccacaaagtt cttattcttg tttccaagaa tttttagcca atgatcgaat agatttcttt
     9541 ttcctaggtg attttaatga ggttgaaatt caaaatgtat tagaatcatt tggctttaaa
     9601 ggtcgaaaag gagatgtgaa ggttcagtat tgtcaacctt attctaatat ccttcaggaa
     9661 ggtatggttc ggaaaaatgt gggacaatcc attttggaat taggttatca ttgcccttct
     9721 aaatatggtg atgagaaaca tttacccatg attgtaatga atggtttact tggtggattt
     9781 gctcactcta aactttttac aaatgtccgt gaaaatgctg gattagctta tactatttca
     9841 agtcagctcg atttatttag tggattcttg aggatgtatg ctggtattga ccgagaaaat
     9901 cgtaaccagg ctcgtaaaat gatgaataat caactgcttg atttaaaaaa aggttatttt
     9961 acagagcttg agttagagca gaccaaggaa atgattcgtc ggtctttgtt actttctcaa
    10021 gataaccaat cttcattgat tgaacgtgct tatcaaaatg ccttacttgg aaaatcttca
    10081 gcagatttta aaagttggat tgcaaaactc gagcaagttg acaaagatgc tatttgtaga
    10141 gcagctaaca atgtgaaact acaagcgatt tactttatgg aaggaataga atgacaaagg
    10201 ttgtttttga agaaaaatac tatccagctg taaaagaaat ggtttatcga actcgtttgg
    10261 caaatggatt gacagtagct cttttgccta aaaaggaatt taaagaggtt tacgggagtg
    10321 taactgtaca gtttggttcg gtagatacgc ttgtcacaga agttgatgga gatgtaaaac
    10381 aatatcctgc aggaattgct cattttcttg aacataaatt atttgaaaga gaagattcta
    10441 gcgatttgat gtcggctttt acgagtctag gtgcagatag taatgccttt acaagcttta
    10501 caaaaacaaa ctatcttttt tcagcaacgg attatttttt agaaaattta gatttacttg
    10561 atgaattgat aacatcagca cactttactg aagcttccat tctgacagag caggatatta
    10621 ttcagcaaga acgagaaatg taccaagatg atccagattc gtgtttattc ttttcaactt
    10681 tagcgaattt gtatcctggt acacctttag caactgatat agttggaagt gaggagtcca
    10741 tttcccaaat caatctaact aatttgcaag aaaattttac aaagttttac aaacctgtaa
    10801 acatgtctct gtttttagtt ggtaattttg atgtggagcg agtacaggac tattttgaaa
    10861 gcaaagaact gaaagattca gattttcagg aagtagcaag agaaaagttg tttttacagc
    10921 ctgtaaagct aacagatagt atgagaatgg aagtatcttc tcccaaacta gcgattggag
    10981 ttagaggtaa gcgagaagtt tctgaagcgg attgctatcg acatcatatt ttattaaaat
    11041 tattgtttgc aatgatgttt ggttggactt cggatcgttt tcaaaaatgt tatgaatcag
    11101 gtaaaattga tgcgtcctta tctctggaag ttgaaataac aagtcgcttt cattttgtca
    11161 tgttgacaat ggatacgaaa gagccagttg ctttgtctca tcaatttagg aaggctattc
    11221 gtaattttac aaaggattta gatattacag aggaacattt agatattatc aaaagagaga
    11281 tgtttggcga atttttcagt agcatgaact ctcttgaatt tattgcaacg caatatgatg
    11341 cttttgaaaa tggtgagaca atttttgatt tgccgaaaat tttacaggaa attactttag
    11401 aggatgtcct tgatgctgga catcatttaa tagatgatgg tgacatagtt gattttacaa
    11461 tatttccatc gtagtaacct atcataatag acactagaaa gaagggatga caagtatgag
    11521 aaaaaaaaca attggagagg ttttacgatt agctagaatc aatcagggat tgagtttaga
    11581 tgaattgcag aaaaagacag aaatccagtt agatatgttg gaagcaatgg aagcagacga
    11641 tttcgatcaa cttccaagtc ctttttacac gcgttctttc ttgaaaaaat atgcatgggc
    11701 tgttgagtta gatgaccaaa ttgttttgga tgcttatgat tctgggagta tgattactta
    11761 tgaggaagta gatgttgatg aagatgagtt gacaggtcgt agacgttcaa gtaagaaaaa
    11821 gaagaaaaaa acatcatttt tacctttatt ttattttatc ctttttgctt tatcgatttt
    11881 aatttttgtg acttattatg tttggaacta tattcaaact caaccagagg agccttctct
    11941 ttctaattac agtgtggttc aatcaacaag ttcaactagc tctgttcccc actcctcaag
    12001 tagtagttct tctagtatag aatcagctat aagtgtatca ggcgaaggaa atcatgtaga
    12061 aatcgcttat aagacaagta aggaaacagt taaattgcaa ttggcagttt cagatgttac
    12121 aagttgggtc agtgtttcag aaagcgaact tgagggcggt gtaaccttat cgccaaagaa
    12181 gaaaagtgca gaagcaacag ttgcaactaa aagtcctgta acaattacgt taggtgttgt
    12241 aaaaggtgtt gatttgacag tagataatca gactgttgat ttatcgaaat taacagctca
    12301 gactggacaa atcactgtaa cctttactaa aaattaagga aaaacgaatg aaaaaagaac
    12361 aaattcccaa tctcttaaca ataggtcgaa ttctctttat acctattttt atctttattt
    12421 taacgatagg aaattcgata gagagtcata tagttgcagc tattatcttt gctgttgcca
    12481 gtattaccga ctatttagat ggatatttag ctcgtaaatg gaatgtggtc agtaattttg
    12541 gtaaatttgc agatcctatg gcggataagt tactagttat gtcggctttt attatgttga
    12601 ttgagttagg tatggctccg gcttggattg ttgcagtgat tatatgtcgt gagttagctg
    12661 tgacaggttt aaggctttta ttggttgaaa ctggtggaac aattttagca gcagcaatgc
    12721 ctggaaaaat taaaactttt agtcagatgt ttgctattat tttcttgcta ttacattgga
    12781 ctttgcttgg tcaagttcta ctttatgtag ccttattttt cactatctac tctggctatg
    12841 actattttaa gggtagtgcc tatgtattta aagggacatt tggttcgaaa tgaaatcaat
    12901 aattgatgta aaaaatcttt cttttcgcta taaagaaaat cagaactact acgatgtgaa
    12961 ggatattacg tttcacgtga aacgtggaga atggctttcg attgtagggc ataatggtag
    13021 tggtaaatca acgacggttc gattaattga tggcttactg gaagcagaat ccggagagat
    13081 tgtaattgat ggccaacgtt tgactgagga aaatgtttgg aatatacgtc gtcaaatcgg
    13141 tatggttttt caaaatccag acaatcaatt tgttggagcg actgttgaag atgatgttgc
    13201 ttttggtttg gaaaatcagg gactttctcg tcaagaaatg aaaaagagag tggaagaagc
    13261 tctggcttta gttggcatgt tggactttaa aaagagagag ccagcgcgtc tatcaggtgg
    13321 tcaaaagcaa cgtgtggcca ttgcaggtgt tgtagcccta agaccagcta ttttaatctt
    13381 agatgaagca acgagtatgt tggatcctga ggggcgtaga gaacttattg ggacagtaaa
    13441 aggaattcga aaagactatg atatgacggt catttctatt acccatgatt tggaagaagt
    13501 cgccatgagt gatcgcgtat tggtcatgaa aaaaggggaa attgaatcaa ctagtagtcc
    13561 aagggagctt ttctctcgaa atgatttaga tcaaattgga ttagacgatc cttttgctaa
    13621 tcaattaaaa aaatctttga gccagaatgg ctatgattta cctgaaaatt atttgacaga
    13681 aagtgagcta gaggataagc tatgggaatt gctctagaaa atgtgaattt tacatatcaa
    13741 gaaggtactc ccttagcttc agcagctttg tcggatgttt ctttgacgat tgaagatggc
    13801 tcttatacag ctttaattgg gcacacaggt agtggtaaat caactatttt acaactctta
    13861 aatggtttat tggtgccaaa tcaagggagt gtgagggttt ttgatacctt aatcacctcg
    13921 acttctaaaa ataaagatat tcgtcaaatt agaaaacagg ttggcttggt atttcagttt
    13981 gctgaaaatc agatttttga agaaacggtt ttgaaggacg ttgcttttgg accgcaaaat
    14041 tttggagttt ctgaagaaga tgctgtgaag actgcgcgtg agaaactggc tctggttgga
    14101 attgatgaat cactttttga tcgtagtccg tttgagctgt cagggggaca aatgagacgt
    14161 gttgccattg caggcatact tgccatggag ccagctatat tagtcttaga tgagccaaca
    14221 gctggtctag atcctctagg gagaaaagag ttgatgaccc tgttcaaaaa actccaccag
    14281 tcagggatga ccatcgtctt ggtaacgcat ttgatggatg atgttgctga atatgcgaat
    14341 caagtctatg taatggaaaa gggacgttta gtaaaggggg gcaaaccaag tgatgtcttt
    14401 caagacgttg tttttatgga agaagttcag ttgggagtac ctaaaattac ggccttttgt
    14461 aaacgattgg ctgatagagg cgtgtcattt aaacgattac cgattaagat agaggagttc
    14521 aaggagtcgc taaatggata gtatgatttt agggcgttat atcccagggg attcgattgt
    14581 tcaccgattg gatccacgta gcaaattgct tgctatgatg ctactgattt tgattgtgtt
    14641 ttgggctaat aaccccttga cgaatctgat tctttttata gcgacaggga tatttattgc
    14701 cttgtcagga gtatctcttt cattttttat tcagggcttg aagtctatgt ttttcttgat
    14761 tgccttcaca actatttttc aactattttt catttctaat gggaatgttt tatttgagtt
    14821 ttcgtttgtg agaatcacgg attatgcttt gcaacaagct gggattattt tttgtcgctt
    14881 tgtattgatt attttctttt caactttgtt aaccttaaca accatgcctt taagtttggc
    14941 atcagctgtc gaagctttat tagcaccttt aaagcgtgtg aaagttccag ttcatgaaat
    15001 tggattgatg ctgtctatga gtttgcgttt tgtcccaacc ttgatggatg atacgactcg
    15061 gattatgaat gcacagaaag ctcgtggagt ggattttgga gaaggaagca tcgttcaaaa
    15121 agtaaaggcg atgattccca ttttgattcc tctttttgcg acaagtttaa aacgtgcaga
    15181 ttccttggct atcgctatgg aagcgcgtgg ctatcagggt ggaaaaggca gaagtcaata
    15241 ccgacaattg aaatggactc taaaggatac gctgaccatt cttgttattc tcgtacttgg
    15301 ttgttgttta tttttcttaa aatcttagta gctttcagga attgataaaa agttactgta
    15361 acagtttttg atataaaggt aggaatatga accgttttaa aaaatcaaaa tatgtcatta
    15421 ttgtttttgt cactgttctg cttgtgtcag ctctcttagc gacgacttat tcaagtacaa
    15481 ttgtgacaaa attaggagat ggaatctcat tggttgatag agttgtacaa aaaccttttc
    15541 agtggtttga ttctgtcaaa tcagatttgg ctcatttgac acgaacatat aatgaaaatg
    15601 aaagtttgaa gaaacagctt taccaattag aagttaaatc aaatgaggtg gaaagtttaa
    15661 agacagaaaa tgaacaactg cgccaattgc ttgatatgaa gtctaaattg caagccacaa
    15721 agactttagc agcagatgtt attatgcgtt ctccggtatc ttggaagcag gagttgacct
    15781 tagatgcagg tagatcaaaa ggtgcttctg agaacatgtt agctattgca aatggtggct
    15841 tgattgggag tgtttcaaaa gtagaggaga actctactat agtcaacctt ctgacaaata
    15901 cggaaaatgc tgataagatt tctgttaaaa ttcaacatgg ctctactaca atttatggaa
    15961 ttattattgg ctatgacaag gaaaatgacg ttcttaaagt tagccaatta aatagtaata
    16021 gcgatattag tgcgggagat aaggtgacta ctggtggatt aggaaacttt aacgttgctg
    16081 atattcctgt tggtgaagtg gttgccacaa cgcatagtac agactatttg acacgagaag
    16141 taactgttaa attgagtgca gatactcata atgtagatgt gatagaatta gtggggaatt
    16201 cataatgaga cagttgaagc gagttggagt atttttattg cttcctttct ttgttctaat
    16261 tgacgcccat attagccagc ttctgggctc atttttcccc catgtacatt tggctagtca
    16321 ttttcttttt ctatttctct tatttgagac gatagaagta tcagagtatc tctacctagt
    16381 ctattgtttt gttataggct tggtttatga tgtttacttt ttccatctaa tagggattac
    16441 aactctctta tttatcttat tgggagcctt ccttcataaa ttgaatagtg ttattttgtt
    16501 gaatcgttgg acaagaatgc tagctatgat tgtactgaca ttcctgtttg aaatgggtag
    16561 ttatcttttg gcttttatgg tagggttgac agtagatagc atgtcgattt ttatagtcta
    16621 tagcttggta ccgacgatga ttttaaattt tttatggatt actgtttttc aatttatttt
    16681 tgaaaaatat tatctataag aacgacatat aaatgtaaca aaggcgtaat atttattagg
    16741 cctttttttg gtatactagt attgtcttta aaagaaggag tatctacgta atatgaagaa
    16801 aaaaatctta gcgtcacttt tattaagtac agtaatggtt tctcaagtag ctgttttaac
    16861 aactgcgcat gcagaaacga ctgatgacaa aattgctgct caagataata aaattagtaa
    16921 cttaacagca caacaacaag aagcccaaaa acaagttgac caaattcagg agcaagtatc
    16981 agctattcaa gctgagcagt ctaacttgca agctgaaaat gatagattac aagcagaatc
    17041 taagaaactc gagggtgaga ttacagaact ttctaaaaac attgtttctc gtaaccaatc
    17101 gttggaaaaa caagctcgta gtgctcaaac aaatggagcc gtaactagct atatcaatac
    17161 cattgtaaac tcaaaatcaa ttacagaagc tatttcacgt gttgctgcaa tgagtgaaat
    17221 cgtatctgca aacaacaaaa tgttagaaca acaaaaggca gataaaaaag ctatttctga
    17281 aaaacaagta gcaaataatg atgctatcaa tactgtaatt gctaatcaac aaaaattggc
    17341 tgatgatgct caagcattga ctacgaaaca ggcagaacta aaagctgctg aattaagtct
    17401 tgctgctgag aaagcgacag ctgaagggga aaaagcaagt ctattagagc aaaaagcagc
    17461 agctgaggca gaggctcgtg cagctgcggt ggcagaagca gcttataaag aaaaacgagc
    17521 tagccaacaa caatcagtac ttgcttcagc aaacactaac ttaacagctc aagtgcaagc
    17581 agtatctgaa tctgcagcag cacctgtccg tgcaaaagtt cgtccaacat acagtacaaa
    17641 cgcttcaagt tatccaattg gagaatgtac atggggagta aaaacattgg caccttgggc
    17701 tggagactac tggggtaatg gagcacagtg ggctacaagt gcagcagcag caggtttccg
    17761 tacaggttca acacctcaag ttggagcaat tgcatgttgg aatgatggtg gatatggtca
    17821 cgtagcggtt gttacagctg ttgaatcaac aacacgtatc caagtatcag aatcaaatta
    17881 tgcaggtaat cgtacaattg gaaatcaccg tggatggttc aatccaacaa caacttctga
    17941 aggttttgtt acatatattt atgcagatta atttacagag ggactcgaat agagccctct
    18001 tttcaggttt taccgtgaca atccctatta aaaattatat caaaatagct tgaaaatatt
    18061 ggaaaagtat ggtaaaatga aaattgtcgt gtgaacgata atactcattc ttgatgaatt
    18121 gtgaagcagt tgcccttggg tcgttttgcg agttgaagtc aagaagagga aaaaaacaaa
    18181 aaggagaaat actcatggca gtaatttcaa tgaaacaact tcttgaggct ggtgtacact
    18241 ttggtcacca aactcgtcgc tggaatccta agatggctaa gtacatcttt actgaacgta
    18301 acggaatcca tgttatcgac ttgcaacaaa ctgtaaaata cgctgaccaa gcatacgact
    18361 tcatgcgtga tgcagcagct aacgatgcag ttgtattgtt cgttggtact aagaaacaag
    18421 cagctgatgc agttgctgaa gaagcagtac gttcaggtca atacttcatc aaccaccgtt
    18481 ggttgggtgg aactcttaca aactggggaa caatccaaaa acgtatcgct cgtttgaaag
    18541 aaattaaacg tatggaagaa gatggaactt tcgaagttct tcctaagaaa gaagttgcac
    18601 ttcttaacaa acaacgtgcg cgtcttgaaa aattcttggg cggtatcgaa gatatgcctc
    18661 gtatcccaga tgtgatgtac gtagttgacc cacataaaga gcaaatcgct gttaaagaag
    18721 ctaaaaaatt gggaatccca gttgtagcga tggttgacac caatactgat ccagatgata
    18781 tcgatgtaat catcccagct aacgatgacg ctatccgtgc tgttaaattg atcacagcta
    18841 aattggctga cgctattatc gaaggacgtc aaggtgagga tgcagtagca gttgaagcag
    18901 aatttgcagc ttcagaaact caagcagatt caattgaaga aatcgttgaa gttgtagaag
    18961 gcgacaacgc ttaatttata caaatagtaa ttacctagga gggcggggct tagcccggct
    19021 ctcctatttt caaaaaatat aggagaatta aaatggcaga aattacagct aaacttgtaa
    19081 aagagttgcg tgaaaaatct ggtgccggtg ttatggacgc taaaaaagcg cttgtagaaa
    19141 cagacggtga catcgaaaaa gcgattgaat tgcttcgtga aaaaggtatg gctaaggcag
    19201 ctaagaaagc tgaccgtgtt gctgcagaag gtttgactgg tgtttatgtt aacggtaatg
    19261 ttgcagcagt tattgaagta aacgctgaaa ctgacttcgt tgcaaaaaac gctcaattcg
    19321 ttgaattggt aaatactaca gctaaagtca ttgctgaagg aaaacctgct aacaacgaag
    19381 aagctcttgc tttgataatg ccttcaggtg aaactcttga agctgcatac gtatctgcaa
    19441 cagcaactat cggagagaaa atctcattcc gtcgctttgc attgattgaa aaaacagacg
    19501 cacaacactt tggagcatac caacataacg gtggacgtat cggtgttatt tcagttgttg
    19561 aaggtggaga cgaagcactt gctaaacaat tgtcaatgca catcgcagcg atgaaaccaa
    19621 cagttctttc ttacaaagaa ttggatgagc aattcgttaa agatgagttg gcacaattga
    19681 atcacgttat cgaccaagac aacgaaagcc gtgcaatggt taataaacca gctcttccac
    19741 acttgaagta tggatcaaaa gctcaattaa ctgatgatgt tattgctcaa gctgaagctg
    19801 acatcaaagc tgaattggct gcagaaggca aaccagaaaa aatctgggac aaaattattc
    19861 caggtaaaat ggatcgcttc atgcttgata acactaaagt tgaccaagct tacacacttc
    19921 ttgcacaagt ttacatcatg gatgacagca agacagttga agcatacctt gaatcagtta
    19981 acgcttcggt agttgagttt gctcgctttg aagttggtga aggtatcgag aaagctgcaa
    20041 acgacttcga agctgaagtt gcagctacaa tggcagcagc cttgaataac taataataga
    20101 aaggaagcaa attgcttcct tttttgtata gggcttagat ttttcctgaa attgtttttt
    20161 ccccagtctc actcgattag aataattaaa acaaaaagcc ctataataag ggctaagtgc
    20221 atttaggaga ctatacttca aattcatagt aagcgcgttc ataacaaggt atctatccta
    20281 attccccaac ttccagtata ctagataaaa aataattatc ggagtaaagg tattgtacta
    20341 tgataattca actaagtgat ttaggtcaag ttcaccttgt ttgtggcaag acagatatga
    20401 ggtagggaat agactcatta gcctatgtag ttaaaaccca ctttgaattg gattctttct
    20461 ccggtcaagt ctttctcttt tgtggtgaac gtaaagaccg ctttaaagcc ctttactggg
    20521 atggtcaagg attttggcta ctatataaac gctttgagaa cggaaaactg acttggctaa
    20581 gtacagaaaa ggatgtcaaa gctctcgcac ctgaacaagt agattggctt atgaagggct
    20641 tttctatcac tccaaaatat agtatattga aactagaata gtacacctct gcttctaaaa
    20701 cattgttaga aatcgatttg actgtcctga tcgatttatc ctgttcttat ttcattttac
    20761 tataaatcta tcagaaagtt gtgatttcta ttgaaatgag gactttcttt ttacactcat
    20821 ctgctttcaa aaagcattct agtccgtctc cgattaacga tggactttat cacctccttc
    20881 tccagtcctt gtatgacatc ttccaaagtt cgaaaggctt tattcttaaa tccacgttta
    20941 cgaatctctt tccacacttg ttcaatgggg ttcatctctg gtgtgtatgg aggaataaat
    21001 gcaaagccaa tattagtcgg aatctttaag gtacttgatt tatgccatat agcattgtcc
    21061 ataacgagta aaagataatc atctggataa gcttgtgaaa tctcctattc ctaaagcccc
    21121 tttagcgcat aactttggct cagcttctat tatcgctcac accatccatc agaagtttaa
    21181 tctgaaggta cccaattatc gccaagaaga agattgggct aggatgggtt taccaatcat
    21241 acgtaaggaa atctctaatt ggcatatcaa gacgagtcaa tactatttgg agccccttta
    21301 taacctcttg cgagagagac tattgactca gcccttactt catgcggatg aaacttctta
    21361 tagggtgcta gagagtgata gtcagctgac ttactactgg acttttttgt ctgggaaagc
    21421 tgagaaacaa gggattacgc tttaccatca tgtcctaatc gatttgttca tatcttattt
    21481 caatccatta taaatagtga gaaatatcta tcctatcttc tagaatgtct tccaaacgtg
    21541 gaaactctcg taaacaaaga ggttttagag gc
//
LOCUS       DAVBGY010000034        21234 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVBGY010000034 DAVBGY010000000
VERSION     DAVBGY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21234
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.034"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(311..2038)
                     /gene="ezrA"
                     /locus_tag="VQ620_001283"
     CDS             complement(311..2038)
                     /gene="ezrA"
                     /locus_tag="VQ620_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HET2763902.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2120..4066)
                     /gene="gyrB"
                     /locus_tag="VQ620_001284"
     CDS             complement(2120..4066)
                     /gene="gyrB"
                     /locus_tag="VQ620_001284"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET2763903.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4081..4644)
                     /locus_tag="VQ620_001285"
     CDS             complement(4081..4644)
                     /locus_tag="VQ620_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2763904.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4729..5283)
                     /locus_tag="VQ620_001286"
     CDS             complement(4729..5283)
                     /locus_tag="VQ620_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET2763905.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5294..6517)
                     /locus_tag="VQ620_001287"
     CDS             complement(5294..6517)
                     /locus_tag="VQ620_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET2763906.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVSLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6554..9004)
                     /locus_tag="VQ620_001288"
     CDS             complement(6554..9004)
                     /locus_tag="VQ620_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HET2763907.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQALGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIIGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9203..10132)
                     /locus_tag="VQ620_001289"
     CDS             complement(9203..10132)
                     /locus_tag="VQ620_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2763908.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10272..10559
                     /locus_tag="VQ620_001290"
     CDS             10272..10559
                     /locus_tag="VQ620_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HET2763909.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10593..11927)
                     /gene="ciaH"
                     /locus_tag="VQ620_001291"
     CDS             complement(10593..11927)
                     /gene="ciaH"
                     /locus_tag="VQ620_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HET2763910.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11917..12591)
                     /gene="ciaR"
                     /locus_tag="VQ620_001292"
     CDS             complement(11917..12591)
                     /gene="ciaR"
                     /locus_tag="VQ620_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HET2763911.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12700..15246)
                     /locus_tag="VQ620_001293"
     CDS             complement(12700..15246)
                     /locus_tag="VQ620_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET2763912.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHIAYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15370..15810)
                     /locus_tag="VQ620_001294"
     CDS             complement(15370..15810)
                     /locus_tag="VQ620_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HET2763913.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15785..16654)
                     /locus_tag="VQ620_001295"
     CDS             complement(15785..16654)
                     /locus_tag="VQ620_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HET2763914.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFTELTNTEANCLVGTKEFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDLESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16696..17424)
                     /locus_tag="VQ620_001296"
     CDS             complement(16696..17424)
                     /locus_tag="VQ620_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2763915.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(17432..17911)
                     /locus_tag="VQ620_001297"
     CDS             complement(17432..17911)
                     /locus_tag="VQ620_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2763916.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVDHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVK"
     gene            complement(17922..18620)
                     /locus_tag="VQ620_001298"
     CDS             complement(17922..18620)
                     /locus_tag="VQ620_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HET2763917.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18613..18879)
                     /locus_tag="VQ620_001299"
     CDS             complement(18613..18879)
                     /locus_tag="VQ620_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HET2763918.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(18945..19871)
                     /locus_tag="VQ620_001300"
     CDS             complement(18945..19871)
                     /locus_tag="VQ620_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HET2763919.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19946..20761)
                     /locus_tag="VQ620_001301"
     CDS             complement(19946..20761)
                     /locus_tag="VQ620_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763920.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20765..21085)
                     /locus_tag="VQ620_001302"
     CDS             complement(20765..21085)
                     /locus_tag="VQ620_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET2763921.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLTKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         6418 a         4661 c         3720 g         6435 t
ORIGIN      
        1 ttattggacg gttaccaagt tacttcaaag tacagatttg aagctgcaga taagactgac
       61 gaaatcactt taatactaaa tgaaaatcaa aaagcaaact aagaagctag cctcaggctg
      121 tacttgagta cggtaaggcg acgctgacgt gatttgaaga gattttcgaa gagtataaaa
      181 cactgttttg aagtggtgga taaatcggct gggtaaaaac tcaatctcag gagaaaatga
      241 agtaaatctc cccacaacta tagaaaaaga tttgattctg ctcctcacac aataaaatct
      301 ttttctttta ttaaaaacga atcgtttcac gtgttttctc atatgaggta acaaagcgat
      361 tggttacacc aggctctgcc acttccaatg cttgagaaat cttgtcaaat gaagcgtgat
      421 aatcaaattc tttttcaaaa atatctaaag cttcgttaaa tgcttcttga atgcgttcat
      481 caaatgagcg atagcggtta gaatattgca agagttgctc tgtcaaagtt gcatattgta
      541 caatattata agtttccgtt tctaaagctt ccatatcatt cgttgcaatt tcaagaactc
      601 gggtaacaga ttcaatgtta atcatttttt gttctaactc aaccattaaa tcctcggtat
      661 tattgcttgc cgtaaagaat aacttcaaga aagtttgtgg aatacctggc agattgcgtt
      721 tttccatgta tcgcttgata gtatggagac gattgacata aacattggcc ttttgacgtg
      781 cattaatatc atctttctca atttgtgtca ggcgctcact aactgaaatt tgctcatctt
      841 caatatcttt tagttgagtt tgtaaatcct caagattttc ttcaagaact gaataagctt
      901 gggttggttc ttcttgattt gaagttacct caacaatagc tgcctcaaaa ctctctaatt
      961 ctgtctgaat acgacgaaca tggcttgcag ctgtctcagg aagtaaatag gtcttgttca
     1021 aacgtgcaat atcttctccc aataaagtat tattctcttt catatgttga aggtaagttg
     1081 gaagagttgc aagtagattt tccactactt tctgagcagc aatttctcga gtaaaaatat
     1141 catacaaggc attgatttcc tcttgtgcct gtccattctc atattcggca ttatccaatt
     1201 ccaactgacg aatattctct tggtttttct tgaatgcttc ataaagcaag tggaaacgcg
     1261 cttcaatatc cgtttcaaca aaatgataat tagcatcaat tagtttacga taaccggctt
     1321 ccaaatcctg taattgatct ggcaattctg tagaaagcgt cgtaaccaag gctggaacac
     1381 gatccacaat atgacttaag gccaaaatgt gattttctgt attatccaaa atcactgcgg
     1441 cttccacagg gtcacccgat gaattcaagg ttacaaattg tgaaaattca gattggatat
     1501 tttctaattg tttttcaatt tcatccaagg cttgaccata ctgttctgaa ttttcagcaa
     1561 ctctatgctg aagttcctca aataaatcca aagcatgaag aacacgacca ctatttttag
     1621 attcttgctt ctctaagtct gccaaagcat tgcgaattgc cgcaatatct tcttcaatca
     1681 aagtaatttg actctcaatt tggtcaattt gatgactggc cttgagaaaa cgaaatgaat
     1741 ggttataacc ttctgcttca aagagattat tttcaatatc ggcaaaagag ttgagagata
     1801 aatcgaccca tttttgattc cattcacgga aagccacttg actttgtcca atcaagtgca
     1861 tattttttac agcttctact tcatcattta ctggaagatt gtatagttct tcttttcttt
     1921 cttctagcgc ctctaatctc ccctcgtttc gcttacgtag aaagattgcc actacataag
     1981 ccagaactaa aatgactgca attgcaacca ttaaataaat tagttgtcca ttagacatat
     2041 caaactcctt ttttacttga aacaatcgta acgattatat catatttttt agaccaaggg
     2101 aactacttct ccctattttt tagacatcaa gtgtactata gacagcattt tcttcgataa
     2161 actcacgacg aggctctact cgatccccca tcaacatatc aaagatttta tctgcttctg
     2221 cagcatcatc tacagaaact ctagccatca agcgatgttc gggatccatg gttgtttccc
     2281 acagctgatg atcgtccatt tcacctagcc ccttataacg ctgaatagtc ggtttggtac
     2341 gaccttcact ataacgggct aaagcttctt ggagtttgat ttcttgatct gcacccggct
     2401 ggatatattc tttaatctcg cttccaacct tgacaccata gattggtggt tgggcaatat
     2461 aaacataacc agcttctagg attggtttca tataacgata aatcaaggtt aaaagaaggg
     2521 tacgaatatg ggctccatcg acatcggcat cggtcatcaa aacgagtttt tggtaacggg
     2581 ctttcgaaac atcaaattct gcgccaaatc ctgttcccat ggctgtgaaa agactacgaa
     2641 tttcttcgtt agctagaatc ttatccatac ttgctttttc aacgttcaaa atcttaccgc
     2701 gaattggaag gatagcctga aactcacggt tacgaccaga tttggctgat ccaccagctg
     2761 agtctccttc gacgatgaag agttctgttt cagcagggtt attagaagaa cagtctgcta
     2821 gtttccctgg aaggttggaa atttccaaac cagatttttt acgtgtgact tcacgcgcac
     2881 gcttggcagc cacacgagcc ttggcagcta aaatcccttt ttccacgata cgtttggcaa
     2941 tctgtggatt ttccatgagg aaatcggaga aggcttcact gaagaggcga ttggtaatct
     3001 tgaccacttc gctatttccc aatttggtct tggtttgtcc ttcaaactgt ggatttgggt
     3061 gtttaactga gataactgca gttaatcctt cgcgaacatc ttcccctgtt aaattatctt
     3121 cattgtcttt cagtaactta ttcttacgag cataatcatt gataacacga gtcagtgccg
     3181 tacggaaacc ttgttcatgc gttccacctt catgggtatg aatattattg gcgaaactca
     3241 tgacattttc atggtaacct gttgtgtact gcatggctac ctcaactgtg atatcatcca
     3301 tctcaccgtc tgtatagatt ggtgtatcaa agattacatc cttgttctcg ttgatatatt
     3361 caacgtaact agcaatccca ccttcataat gataatgctt ggtttgttcc aaaccttggc
     3421 gcttatcagt gatagaaatt tgaagaccgc gatttagaaa ggccaactct tgaatccgtt
     3481 tatttaattt atcaaaatca aaggttgttg tttcagtaaa gatttttggg tccggtatga
     3541 agtgaaccgt tgttccagtt ctatctgtat ccccaaccac ctcaaggtct gcaacaacat
     3601 gaccacgacg gtattcttgg taatgaatct taccgttttt atggacatga acgtctaatt
     3661 gagtggaaag ggcattaact actgacgacc ccaccccgtg aagaccacct gaaaccttgt
     3721 atccaccacc gccgaacttt cctccagcat gaaggactgt aaagacggtc tcaacagcag
     3781 gacggcctgt tttttcctga atatcgactg ggataccacg cccatcatcc acaacagtaa
     3841 tcgaatcatc tggctcaata aaaacttgaa tatggctggc aaatcctgcc aaggcctcgt
     3901 caattgagtt atcaacaatt tcccagacta gatggtgaag accttctttt gaggttgatc
     3961 caatgtacat ccctggacgc atacgaacag cctctaagcc ctctaaaact tgaatttgac
     4021 tggcatcata atcctgtgcc tgcagatttt tgatttcttc tgtcatctta ttcctttttc
     4081 ttatatgtct aatacttgtc ttaaattcac gatactggta aacaagtggg tatcaagtcc
     4141 tgcagcttga cctgcttcga tatcaatcgg ccgatcacca atgacaagac cagagctaat
     4201 ctgatacttt tctcttaaat aaagcatgga ttcgggattt ggctttctct taaagcctga
     4261 gctagaagtc accacttctg taaaataagc tgctatagag gttttttcta aaatttccaa
     4321 aacctgatca tttcgatgag agaccaaaaa atgacggcca ccttgattta aaatgtcttc
     4381 caataggtca gaaactcctt caaataaaat cgggtgttca agctctctgg cttcattttc
     4441 cttgtacttt tctaaaaaat tctctaaatt gggagcgaat gtctcaatcg caaaaggagt
     4501 agaaaccttt aaagcttgat agacactgtc atggtcttgt gtgataccat acagtgccaa
     4561 tgtttcaaca aatgcagctg ttgaagtttc ataattatcc agtaaagttc cacctaaatc
     4621 ccagatgtaa tcgtgatatt tcataccctt cattatagca tttttttatg aaaaaagcga
     4681 tgatttccct gagttttcca cataaaaaac gagagaataa ctcccgtttt actgattttt
     4741 accaaagaca tctcgataga gcattcctgt tcgtaaactc tctgcgtccc ctcctagttg
     4801 ctccaccaac tcgtaggcaa aggcaagggc tgttgaagga ccccgactgg ttgtcaactg
     4861 accatctacc actactgttt ccttgacgta gtgaccatca aggatttgct cttgaacgcc
     4921 gtcataacaa gtgtatcgct tatttttcaa tatctctgct tgattgaggg caattggtgc
     4981 cgcacaaatg gctgctagtt tcttcccttc ttgctcgaag ctttgcaatt cttgaatcaa
     5041 ggtctgatta tcacgtaaat gtgcagaacc aggcatacct ccaggaagaa caatcatatc
     5101 atagtctgat aaatctccat caaagacatg atctgctctt acttggattg catgcgaacc
     5161 cgttacttgc tcttcaaaac caaccatatc acatgtgata ttggctcgac gcaagacatc
     5221 tacaactgtc aaggcttcaa tttcttcaaa gccctgagct aacataactg ctacttttac
     5281 catgattttc tccttattta atttgtttta atacaacctt tttccgtttg aatgggactt
     5341 ttccgctctt ttcttcagct agattagtct ggtaactcat cgataaaagc aaaccaacac
     5401 caatcagatt actgataata gctgatcccc cttgcgaaat gaaaggcaag ggaatccccg
     5461 tcaaaggaag tagtccagtc acagcaccga tattctcaaa gatgtggaag agcaacatca
     5521 taatcaaacc tgtggaaata taagtgtaga actggttatt tgatttaaga gtaatcttca
     5581 acatacggta aatcaacatg agatagaggg caataaccag gacagagcca ataaagccaa
     5641 aatcttctgc aataaccgta aaaatcatat ctgactctcg aactgggata agcagattcg
     5701 aagcattaaa tccctgacca aataagccac cactcccaat ggcaatctgc ccttgagcct
     5761 gctggtaagt cgttgtttgg gcaaactcaa agggattgag ccaagccaaa atccgattga
     5821 tttggtaggt cggcattcca atctggtgaa gaaaagctcg tccgtccttg ctaataaaga
     5881 tagctaagaa accagcaact cctgttacag cagtcacaaa tactgggata ataattttcc
     5941 aagaaacccc tgataataaa acgattcctg agaaaatggc tacaaaaacc aaagccgtcc
     6001 ccaagtcact ttgaagtgct aaaagaacta ggactggaat ggtaaagaga atcatccaga
     6061 aaattaacaa aaagtccagc gaaaccgtgc gtctccattc cttatgtttc tttgtaaatt
     6121 ggacaatgac acgagccaac atgaggatat aggatatctt cataaattct gacggttgaa
     6181 atagggtaat tccatttatt gatacccagt ttttggcacc cgttgatgca actaagcttg
     6241 gattataaaa tacaatcggc aagatcataa gtcccaagcc taaaatatat agaaaggggg
     6301 tcaccttcca aagaaattct gtattaaaga gcatgaccac aaaaccaatc acaagcccca
     6361 aggcaatcca ggcgacctgc tgccctaaaa tgggcagaat attattggga taatcatgac
     6421 taacggctat atagatagcc accacaccga tgaccagtag aaaaaatact ggcaagagca
     6481 aactataatc gactctagag tcgagagaac gtttcatata tactaacctt atactttcat
     6541 acaatactat ttatcaaagt tcattaaaaa atctatcaat agcctcgtca acttcggatc
     6601 gagagatggt tttaacagtc gcttcttctg ctagagatgc tactatttgt ttgccgtatc
     6661 gttttccgat gattctccta tccaaaataa gagttaagga acgttggtat tcacgtctca
     6721 tacttcttcc caaagcctgt tttaaacgaa taatggccat tggcaattga taatcataaa
     6781 aggcattttt cccttcttga ttcagttctt gattaatctt tttcgtcaag ggttcttgag
     6841 gattttggaa aggaagcctc ggtacaactt gaatcacaaa aggatggctt gaaaaatcaa
     6901 ctccctccca gaaacttgct gcaccaagca agatttgttg ttcacctttt tcaaagcgtt
     6961 tctttagctg atgaacatcc ccatttttat actgggccaa gtggctaact gtaagtaaat
     7021 ccgatactgc tagaagcatg tctttagcgg taaagagaac caaaatcggt tgctggaaag
     7081 cttgaatttc cactagtaaa gcagctacct ctctggcata gacttctaag gaggtttctg
     7141 ttaccagagg gaaatctttg accataacca cttcttgttc ctgttttccc cgagattcaa
     7201 tcttgacaaa tttggcttca ggatagccta aaaggtctgc caaagaaacc ctctgactaa
     7261 tctcaagagt agccgatact cccaaggctt ggcaactctc aggaagtaaa gaggacagaa
     7321 gagtacggcc tgattttgta gaagaaatct gaattttctt ttgactcgtt tcagttactt
     7381 caagccagta atcaccttca gctgtaaaat agcgaaccag ctcatcaaag ccttctactt
     7441 ctaattctga aaaatactga tggagattgt ccagagaatc taagatattt ttcctagatt
     7501 taccagactg aaattgttct atcaagtaga gacactcaaa gcgaatactt tctagtatcc
     7561 gttgttgaac cctgttttct tctcctacta aagccttatc aattaaatcg ataatagatt
     7621 gtatatcgta ggtctcttga agcagatttt ctagagctaa caaaatcttt tggacttcat
     7681 caataatcag taaacggtca ctgacaaatt caggattatc ttcaagtctg gtcacgagat
     7741 aggcatgatt agtcactaaa agcttgcaag tctctgccct ttcttgacta cgtttccaaa
     7801 aatcttccgt cacaaataag ctctgggatg ataaattccc atcatgacga aggtctgcta
     7861 gaaaatgttg gtaacgatag agttgcccga tttcatccaa atctcctgtc tctgtctcag
     7921 taagccagac caagacttgc attttaaagc gtctaaataa gcgattttca tcattttcct
     7981 gcaaggaatg ataaaaggca tccaacttca gataattttg tggtcccttt aagctatgaa
     8041 tatctgtatg gaacacttcc ttgaggcgtt taccttcttc ttccatgatt tgattttgaa
     8101 gaatctttgt cggaacacta agaacaattt gtcgctcttt ggattgagat aaagcgggta
     8161 agagatagcc ataggttttc ccaatccctg tcggcgcttg aatcagagag acaggttcat
     8221 ctttcaatag caagccaacc tctttagcaa aactttcttg ttcctccctc acttcaaggt
     8281 tcaacagaga aatattttta gaaaagtctt gagatagttt tcgtggctcc agagaagctg
     8341 ccgttttctt aaaatacaga ccttgaactt ggaccaagtc tggagaactc aggatagatt
     8401 ggttgcgata agtttcctca ataaccaggt aggactcata taggagagcg tcagccattt
     8461 ccagcaagcg ttccaagaga cctttaggaa gctgagtcat cttttcccgt aaaaaaagaa
     8521 gtaattctgc tgtagcttgg gcatctgaaa gggctgtgtg tgcgtgttta agaggaattc
     8581 ctaattctcg acacaaaatc ggcaagctat atttttccag ttcagggaaa aagacctggg
     8641 ccaattcgac cgtatcaaca cgagggtttc ttagctcata gccttcaaaa aataaatttt
     8701 ccgccaagag attagcatca aactgaacat tatgggctac aaaaatccca tcctccacca
     8761 agtcaaatat ttttctggca acttgcgaaa aatcaggtgc ttgcgccaga cgttggtctg
     8821 tcaatcctgt cagttctttg atatgagcat ccaagggttc atgtggattg acatccgtcg
     8881 tatagtgatc gacgatttct ccgtcctcaa tcacgacaat tcccacttgg ataattttag
     8941 ccttactacc tgtgctagtt gcctctaaat ccaccacaac atagcgatta ccaatcgttt
     9001 tcattataaa aaattatacc aaaaaaaggg ccatttggca cctgcaaaca taggataatt
     9061 ttcaaactca agaacactac ttttcttaaa aatcccatcg aatagagcat tctagcctgc
     9121 tgatttttta aaatagtgaa gaaatacgaa aaattgagag aagaaatgtt ttcatcttcc
     9181 ttctctcaac gaatcatgta ttttatttaa ccatgtcagg atcgtagtca taaaatcctg
     9241 tatcaaggaa acggttttta gggtgaagtt tacgaacttc ttcatccagc aagaaggctt
     9301 ggtcatggct aaagttgccc tcttgaatca agctacgcgc ttggataaag agttttgcaa
     9361 tctcaacaaa gttctgacca ggagtgtaga gcccttctag tttccagcga gtaaagccat
     9421 gctccaccaa ttctgttaat ttgatcatca aatcaaggtc attgttggca aagatatggg
     9481 tcccatgatt atcttcaaaa atggaatagt ggctctctgg atcacttggc tcagccaaga
     9541 agaggtcacg tttatgcgtc ttttcatcat cgatatgtgt aaagttatag tagttttgca
     9601 agagtggacg tttagaatga tggatgacgc tagcaccgta aaccaaaact tcagcaggaa
     9661 tttccaaaat ctctggcatt ttgaaaagtt cagctgatgg aatttcacgc gccaaaacag
     9721 cctcagatgc gccagccttt tgtccccaga agttaatctg acgactgcta gttaccatgg
     9781 ttgaagcatc gtagatggtc ttaaatgaat aaccatcgcg gttaactacg taaaagacgc
     9841 ctgcatcccc aatcgtaata tagtctgtct tgatttcttc caagaagtct aagaaaggct
     9901 tgatacggtc catcatatct tggtgcatga gagcattgac cgcaacgagc aattccttac
     9961 cagcatcatg aaccaactta gcgatttcac gtaattggtc ataactaaag gtcgttggca
    10021 gacgaaggcc aaaatctttc tcaccgacat agatacggtc tacgccagct tcgagtagtt
    10081 gttcaacttg ttcaatactt tcagcagttg ctgtaatgac aatcttttcc ataagaaaaa
    10141 ttataccaca tttctccaaa atcatccatg ctttgaaaat gaaaagggac tttattcttt
    10201 ttgtaacctt tctgtaataa ttctctgccg aaaaaatgat aaaataggta tgtactgtta
    10261 aggagagaat tatgcccgta agaaaattac aatcctatga ggtagactat caagaagaat
    10321 taaaccagca gcttcctcat taccaagctt atacacctga agcgcaatct gatgccaatc
    10381 tcaaggaaat cctatttttt attaatatcg ctgttttttg tatctgtatt gctatcttta
    10441 gttttatctt tttagcatta aaattatcaa ctgctcttgc ctttgccgca gcaatcggat
    10501 tcagcttact tgttttaaaa gttcaacggt ctattatcaa acgaaaacgt agaagataaa
    10561 tccaaaatat cgccccagtt ggagcgatat ttttattttt tctttttaga tggtgtctga
    10621 atggcaatct tcacttcaaa gattgttccc ttgggtttat tatctttgac agtaacagtt
    10681 ccttttagag catctacaat ttgcttggct agggataatc ctaaaccaaa accacctttt
    10741 tgccgagttc tagccttgtc tactcgataa aaacggtcaa aaattttctt tttatcttct
    10801 gtcgaaatac cgattccatt atcagaaaca agtaaataaa gattgcgatc ggtcgccgag
    10861 ataagaaaat caatttcacc atcctcctca gtatacttga cggcattatc gaaaagaatg
    10921 gtcatcagtt gtttcagaag aagctgatct gtgacaattg ttcgatggat acgattttca
    10981 aaacggaaga cacgattatt ttccgaagca atcatctcgt agtttgtgaa agttgtatta
    11041 aaaaagctag ttggaacttc tgcaagctcc ggcttaatcc catcatctct ccgagctaag
    11101 ttcagcaagc ttgtcgttaa aaaacgcata tttcggactt cttccaaact cgatgcaatg
    11161 ctttcgctca catccataat ggtagcttct ggcttacgaa aaagggtctc taagcgattt
    11221 tgcaaaactg cgagtggagt tcgtaactca tgactggcat tttccacaaa agactgttgc
    11281 ttctgcatac tctcaagcag gggcctaaca ctgaccctag ctagatagag actggcaagt
    11341 aaagacaaaa tccagaaact agccatcacg accacaatca attgctcatg cttttgacta
    11401 gcctgttcca actgactggt attaatcaag acagcagcat acttgatatt ggttgaaacc
    11461 gaactaatat tggtctccat caaaatcaca cgatagattt cttcctgccc atagctatta
    11521 aaaacctgaa tctggtagat atgtcctagt tctttctttt ctaacttaat cttatccaag
    11581 cccaaaaatc gatttccaga aagaagttga gtaaagtctt tatcaaagag aatgacttcc
    11641 gtattggaac tgacattagg ttttatttct actttactag cgtccgccct agcattttct
    11701 aaatctttaa tctcttctgt tgccctattt atagccagct gaataactgc ttgaggattt
    11761 tcactcagtc catgaagctt atcgtccacc gaagtataaa gactcgaatg catgacttgt
    11821 aaaataatca gagtcattgt agaaaaaatc agggtgaaga caccgaagtt gcggataaaa
    11881 taactaaagt catccgcata ccatgttttt ttaagtttac tgaacatctt ttaaaagata
    11941 cccaacacta cgcaaagttt gcaaattctc tgcaaaagtg gttcccttta atttcttacg
    12001 gacttttgaa acatagactt cgacaaccga aatcgttgta tcactatcaa atccccatag
    12061 acggtcaaaa atctgcgtct taggcaaaat cacattttga ttttgaagga aataaactag
    12121 taaatcgaac tctttcccca gcaattcgac aggagtatct tcaactttaa cggtattggt
    12181 tgataaatta accacgatat tcccataagt caaggtgttt tcattaaact tccctgaacg
    12241 tttgagaagg gcctgaatcc gcattttaag ttcttctagg tagaaaggtt tggtcagata
    12301 atcatccgct cccagttcaa atccatgtcc cttgtcatcc aaactttcct tggcagtcat
    12361 aatcagaact ggtgtcgtaa ttcccttttc acgcaattct tttaagactt ggaaaccatt
    12421 tttttctggc aacatcaaat ccagcaaaat caagtcatag acaccactct cagcttcgta
    12481 gagaccttct tctccatcaa atacctgcat aacatccgca aaatcgtcta aaaagtcaaa
    12541 tactgaattt gacagaccta ggtcatcctc aaccaataag atttttatca tgagaaactc
    12601 ctccttatta aaactattat accaaatttg ccttaaaaaa aactcaactc tctgcatttt
    12661 acatgagata gctgagtttt ctttttattt taggcttatt tatgcatttc cgtattgaag
    12721 aacaactgct tcgactgcag ctttttcacg gctaatcaag tcaacacgcg ctgcaatttc
    12781 cttgattccc ataccgatgt tacggctaag agcaaggtca gaaagttgcg gctcaaagaa
    12841 ctccttgtat tccgccaagc gttgctgagt cttaaataca tgagcaggaa ggataacaaa
    12901 gctatcaaag ctcatatctc ctccaagggc tgccttaatc caagcccagt tttcacgcgc
    12961 ccaagaccaa gctgttttct gagttgcttg atgagctagg aattggtaat accaagcaga
    13021 caagtcctgt ggtttgacca caaatttgtc cttccaagaa gtaatcaagt tttggatatt
    13081 atccgcatct gtactgtatg caagagctgc tgccaactgg cgtttaaaga cagcatctgt
    13141 tgcgtgagta taagtatcaa gataaagtgc taacaagtct ttagtctcat gatgtttcat
    13201 ctcattaatc agaacttgtg aacgaatagc tgctgggagt cctgcaagat tctccttgtg
    13261 tgttgcgaag atttggctag cgacttgact agcttctgca tcatttgagc gaatcatcat
    13321 cgaaacagcc agctgacgaa ccaattcatc ctcatctgat tctccgtctt tagcttcaaa
    13381 accaagacgg tcatagttat gacgagccaa tttagcaacc agtcctttga aggctgtttc
    13441 agcatccgtt ccttcatcaa taaagcgctc aagggctgaa atcacttgag aaacagctga
    13501 aaccaccaga taagactctt ccttagcaag tttatcaaga actggaagca agtctgcata
    13561 agcaatgtgt cctgcctcag ccaacaaacg acgttcttga acaatttgca gtttgcttgt
    13621 gttatcaagt gtctctagct cagcaagaac agcttctaac aagtctcctt gatagtcggt
    13681 aatatagtgg gcagtatttt cagtgttgag acgaagagct ccttcatttt cagcaagaag
    13741 agctgcgtag ccagggattt cgatactttc agtttcgagt gtatcaggca agcctttcca
    13801 gttgctattg aggggcacca cccagagacg gttcttgtct tcgttctcac cgatgaagaa
    13861 ttgtttttgt gaaatcttca agacatcatt ttcaactttg acagtaagaa ctgggtaacc
    13921 aggctgttcc aaccaagaat ccatgaaggc tgcgacatca cgtcctgacg cttgaccaag
    13981 ggcatcccaa aggtcactac caatggtgtt gctgtattgg tgtttttcaa agtaggcgtg
    14041 caaaccttta gcaaaatcag catctcctag ccaacggcga agcatgtgca tgagacggct
    14101 tcctttggca tagacgatag cgccgtcaaa gagtgtattg atttcatctg gatgtttaac
    14161 ttcgacgtgg acagactgaa cgccatcagt agcgtcacgt tcaagagcaa gaggtactcc
    14221 acctgtttgg aaatcttcaa agatattcca gcttggttcg atggtatcca cacagacgta
    14281 ttccatcata ttagcgaaac tttcattgag ccaaaggtca tcccaccatt tcatagtcac
    14341 gaggttccca aaccattggt gagccaattc atgggccaca acaagggcaa cttgttgacg
    14401 gctagcaaat gtagagttct catcgacaac caagtaaact tcacggtagg tcacaagacc
    14461 ccagttttcc atagcaccag ctgagaagtc aggaagggcg atgtggagag attgaggaat
    14521 tgggtactta actccatagt aatcttcgta aaactcgata gagcgaacag cgatatccag
    14581 tgagaaatca agatttgaaa gtggatgtgc tttggttgag tagacaccta ccagggtacc
    14641 atttttagtt ttagcggtca ccccttgcaa atcaccagca acaaaggcca acaagtaaga
    14701 agacatgcga ggtgttgtct caaacttcca gatacctgtt tccttacggt tttcaacatc
    14761 gatttctggc atgtttgaca aggccaattc accttctgct tggtcaaagc gaagagagag
    14821 gtcaaaagtt gctttggctt caggctcatc cacacatggg aaagcttcgc gcgcaaaatg
    14881 gctctcgaac tgagtagaca agacctcctt cttgactcca tcaactgtat aataagaagg
    14941 gtaaatccct gtcatgttgt ctgtaatttt accagaaaag gcaagaacca attcaacttg
    15001 accagcctca gccaattcga tatgaagggc ttcattgtca tggtcaactg taaatggacg
    15061 agcttgacct gcaacttcta cagaggtgat ttccaagtct ttttggtgga gggagatgcg
    15121 gtcactctgt gcttgaccag tgatggtcac tttcccagaa aaagtcttgg tctcacgact
    15181 caaatctaaa aataaatcat aatgttcagg aacaaattgc ttaataaaat gttcaactgc
    15241 ttgcatagtt ttctcctatt ctaagtttaa gagctactgc tcttcttcaa acaaacttat
    15301 tatatcatat tttgcttgag aaaaaaggat tggacagcga tttccaaaac tttcttcctt
    15361 ctaaatagtc tacagtgggt agaccttgcg aaattcttct aaaacaacct tgctttcagg
    15421 tgtaaaagtc agccctactt cacccaaaca gtctgtgaaa aagtcctcat gaacctggcg
    15481 ccaataggca agtgatttgt ctccctcacc ttccttaaag gcatgttgcg cagaaacttg
    15541 attgaagagt tcaacagaaa cctttgtaat ttcgacaatg cagacagact gattttgact
    15601 atctaaaatg acatcgaagg tccctacttg gggaagtggt tcgtcttcta gtacatagag
    15661 gtcataggct gatgctgttg ctgtcttttc tcctttaaac accaaatccg ctaaaaggtc
    15721 tggttcaact ccaaaagccc aagcatcgat ttcatctccg atcaaaggat tgatttgctt
    15781 gtatttattc cacatttctt gcggtatcat gggtactcct ttgtaatttt ttactttctt
    15841 cttttatgtg tttaagatga tctggatggt caatctctaa atcaaaaatc tctggaatag
    15901 aactgtagtg gataatgcac ttgataccca actgattcat tttttgtatg aaagaagtat
    15961 tcagatagcc tgctacagca aaattaatct tgttctttct tgctttatcc tgcatatctc
    16021 ttagcatatc taacattatt ggactttcca gatcatgcca ttgactgttt ctcatagtcg
    16081 caaaaacaaa ggaagtcaaa tcattcattc caactacaat ctttgaaatg cccgtttcca
    16141 gtatactaga taagtcaaaa tacgctgacg gtaattcaat catcgttccg actttcccag
    16201 taaagccata ctgacgcaat actgtaatag cttgttttaa ttggtcggca tcattgacaa
    16261 aaggaaagat aacagataga ttggggttgg tttgataaac ttctgtaacg acatgtgctt
    16321 cagcctgaaa ttcatccaaa cacgccagta aacgcctggt tcctctatag ccaaacaagg
    16381 gatgcccttc gtcaaaaaac tctttagtcc ccactaaaca attggcttct gtattcgtta
    16441 attcagtaaa acgataccaa acttcttcat ctgagtacag ggagcagata gtctctagat
    16501 aatcttttac aaattgctga caacttgcta atatgatgtt ttgattaagc tctctcaaca
    16561 aatattcccc acgaatcatg ccgacgtggt gaaatagttg aggataaatt ttttcaagaa
    16621 ttttttcgcc actaagggca agttgatttc tcatagccat tcacctcgtc ttaaaagata
    16681 caatatcata tataattata tcaaaatttc ctctagttca aaagcttcat aaggttcaga
    16741 taactcatat cctaaaaatt ctgctacttt tttggaagcc tcattatgag catcccaaag
    16801 tggaaacata tctctattta aactctctag aatcattgca gcaccaagct ttttagcaaa
    16861 tccatttcct tgttcgtttg gtctagttgc aacttccact tcaactgctt tacggtaaac
    16921 taaccctgag gaaatcccag caatcagttc attattttta agaatcacaa agccaaatcc
    16981 accttttaaa acaaaatcct gataggactc aaaatccccc tgtaaatcct gtgaccattc
    17041 ttccgtagaa aagcagttat aaatatgatt atcaataggc acaatattgt aaccttcctc
    17101 taaatgagta actagattat ttaaaaattc aacttgaaaa tttgccttat ctttaaaaga
    17161 atagcgagta aaagaattta gccccacttt tcgatgcaga aaatcttgcc aattagtatt
    17221 ttctgaaata ataatcttat aatctaaacc atattttcta acaaaatctt cccaaaatct
    17281 ggaatccaac tcccctgcta ggtatagaaa attcccaaca tcataaaagc ttgttgtcct
    17341 atcattgttt cggtagacag ttccaatttg agatttaaga ccatagagaa ccatattttt
    17401 cttccatgtt ttaaatagtt ccattatccc tctacttcac cttccaattc atgtaagaag
    17461 tcttgtccag ttctggaaat cctaataatt cagacttaac cttcaagact aatggcgatg
    17521 cattttcttc tgtaatctct tgaatatcca tccaaatata tccaagtgaa tcattcgcac
    17581 catcagacac agcttccgaa gtcgtaactt gaggtgcact ctcattcatt tcaacatcat
    17641 acaaggccat gacatggtca accataaaat ttgttaactc ttctctgacg aaaacatcgt
    17701 agattcgagg attagagtag cttctaacag taaatcccgt ctcttccata acttctctag
    17761 tcagcgtttc cgtcagtcct tcaccaagtt gctgactgcc tccaggtaga tcataccgat
    17821 gttgataagg gcctctcgtt ttttcaatgc agagtaactt cccattttct aagcaaacag
    17881 cgtagactcc aaagtgtttt ttgatttcca tccaactcct cctacttcaa agaccagcca
    17941 ccatctattg tcaagatttg tccttgcatg gcgctcgctt ttccacttgc taaaaaaaga
    18001 ctaagctctg ctatttcctc tggctcaatc cagcgcttga ttggagtttc actagccacc
    18061 cagtcagcca aaccacctgg ttcaaaatcc gcagcggtca tagctgtctt gactgctcct
    18121 ggagcgatac caaagacctg aatcccagct tcagcatagt ctagagccaa ctgcttggtg
    18181 aagccagcca aggcatgctt ggatgaagta taggcgtgac cacctccacc tgctaggcta
    18241 gaagcaatgg aacacatatt gatgatgatt ccctttttat tttccagcat ttgtgtcaaa
    18301 taataccgag tcaactctac tggaataatg tagttgattt caaaaatctc ttgaatgtcc
    18361 tgcgccgttt gttccaacag tggtttgtaa tcatccaaaa ctccagcagt attacacaaa
    18421 acatccacct gagggcacca gtcaaaaata ggttccaagt ccaaggtcaa atctctctgt
    18481 aaaaagtgaa aatcaccctc taagagtggc ttttcacctt ggtcaactcc ataaacttga
    18541 tagcccttct ctaaaaagag gcgagcttga gccaatccga tccctgaact cactcctgta
    18601 atgagtacac gtttagtcat gcacttctac ccaatccgtt gccaaaacat cacaaactgt
    18661 cgggctccac atggaaaaac cttctccttc gccagaaacg ttgattagga aataaggtgt
    18721 catttcaagt gcaagcccat tttgctcgat ggtatcaaag agttggacat agttttccgc
    18781 acctccccaa ccagttcgta catattttct cttagccttt aacccaggca ggatctcttc
    18841 aaatgtcatg tttttctcct ttaattctac attcttcatt taattatagc aaaaaaccgc
    18901 tttatacggc tttttgaatg tgagttattc aaacctgcta ctacttacgg caaattattc
    18961 cctgcagcaa gataaatttc ataccattct tttcttgtta agctaaagtt tgccgctcgg
    19021 ctaacttctc tcaagtgctt aggatttgtt gtacctacga ctgcctgcat ttttgctgga
    19081 taacgcaata tccaagaaat ggcaatagtt gaagaggtta ctccatattt aatagctaaa
    19141 cgatcaagta cttgatttaa agcttgaaat ttctcatttc caacaaaatt ccctttaaaa
    19201 tacccgaatt gtaagacaga ccatgcttga atgaccacat cgtgtaattg gcaatattca
    19261 aaaatgctgc catctcgcat agctgcttga ctatcttcca tattaacatg aaaagctgat
    19321 tcaaatcctg gagtaaaagc cgcactcaat tgtagctgat taacagctaa cggctgcttg
    19381 acatcttttt taagcaactc catcatcata ggattttgat tagaaactcc aaaatctcga
    19441 actttacctt gtttataaag gagattaaag gcttctgcta cttggtcaga ttccatcaaa
    19501 gcatctggtc gatgaaggag caagctatct agatgatcaa tcttcaatct ttgcaaaata
    19561 ccgtctactg attttataat atagtcctta gaaaaatcaa aataggtaaa ttcttcaatg
    19621 cgaatgccac atttggactg aatccacatc ttttctctta aatctggacg attttttagg
    19681 acaagaccta acagttcttc acaacgacca cgaccataaa tatcagccaa gtcgaaggca
    19741 ttgattccaa cagaaagtgc tgtttctaca agctcttcaa cttcttttac agacttatct
    19801 tttattctca tcattccgag aacaatttct gataattctt tgtcatcttg accaagagtt
    19861 atgtatctca tcaaattttt ctcctttaat ttctaacatt cttcccttca ttataacaaa
    19921 aaaccgcttt gcaacggctt tttgactata cttcactcca ttttatcttc ttaaacccac
    19981 ggaacaagac aaagattcca ataaagagga cagctaaagg aataactttt gtaaggaaaa
    20041 catttgaaat tcccatccac tcataataac ggagcagaga acccaccaca agatgggcaa
    20101 taatcatact gacaaatgga cgaaagaccg cttctttcca attccaaata ccgataacta
    20161 gcgaaatcgt aaagacagac aaactatccc agggagccgg aatataaaag gctccttctt
    20221 gtatgaagct tgccattcct acatatccta aaacaactag aagaactata gtcccaacaa
    20281 caatgtaagt gccaattttc attttaggag aatcttggac taaacttctt cgtaaaattg
    20341 tggccacaag tccaaatcca atcagaaaaa taagaagttg ccctaaaaat gtgagcaaat
    20401 tgactgttaa gagaggacct ttagaaaaat cacttagtag ttgataataa cgtaatacag
    20461 ccaggacaag aattggcgtc aaaagggact ctttgataga actgcgaggt gctcccttga
    20521 gaatctcttt cattattttt ttaggattct tacctagata atcctctgca ctcatgccat
    20581 ctcgttctgc ttctgagaaa tctagcatca tcaaatagat ctgctctctg agatagtctt
    20641 catcatagag aaatccagca agattaaaac tttcccacaa ctcctcaaaa tacttttgat
    20701 tctcctcaga aaactcatgt agcaaagcgc ttgtttcttc gtaatacttc attttcttca
    20761 tggtttaacc cccattctta atcccttcta ctttttgact caaatcgtcc cattgctgcc
    20821 aaaagactga gacacgctct tctccttctt tcgttaatga aaaatacttc cgatctggac
    20881 catctggcga cgggcgcatg tcgcctctta tccattgatt tttttctaac ttttgcaaca
    20941 aaggataaat agttcctgga acgatagtat caaatccagc ctctcgcaaa gtctgaacca
    21001 actcataacc ataccgctct ttttgaccaa tcatatccaa gacacaacct tcaagaacac
    21061 cttttaatag ctgagtttct ttcatcactt ctcccttcta atctattttg taatacctac
    21121 tagtaacttc acctatagta tattacttct acactagttt gtaaagcata atagttaata
    21181 ctcttcgaaa atctcttcaa accacgtcag cgtcgcccta ccgtatgtat ggtt
//
LOCUS       DAVBGY010000035        21098 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVBGY010000035 DAVBGY010000000
VERSION     DAVBGY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21098
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.035"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            210..686
                     /locus_tag="VQ620_001303"
     CDS             210..686
                     /locus_tag="VQ620_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2763922.1"
                     /translation="MKLSLYSEVEHYHFLSSYSLNDITYTDTPKNIIEKSKKDKYRYP
                     IVILNKDHKVIGFFCLHLNTGPKEYGYYQNDYALIRGFSIHDNFRNQGYGSNALNEIF
                     EFIDSTLKLEINHIILAVNEKNILAQKAYRNSGFSVVKKDLEGKRGKLIIMEKSRN"
     gene            complement(1360..1752)
                     /locus_tag="VQ620_001304"
     CDS             complement(1360..1752)
                     /locus_tag="VQ620_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET2763923.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLTRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTDKSQYEFLFPSVK
                     IKEEKTDKKEEVATDSSEKAEKKIRREA"
     gene            complement(1730..2191)
                     /locus_tag="VQ620_001305"
     CDS             complement(1730..2191)
                     /locus_tag="VQ620_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET2763924.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVGQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            complement(2154..2414)
                     /locus_tag="VQ620_001306"
     CDS             complement(2154..2414)
                     /locus_tag="VQ620_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763925.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            complement(2419..2847)
                     /locus_tag="VQ620_001307"
     CDS             complement(2419..2847)
                     /locus_tag="VQ620_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET2763926.1"
                     /translation="MEGQIVKSMIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVE
                     EQIFFMEFEELYRETQKRSVASQQKTSLNLDGQMISNGSQKLTVPKGIQAPSGQSITF
                     DRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            complement(2816..3142)
                     /locus_tag="VQ620_001308"
     CDS             complement(2816..3142)
                     /locus_tag="VQ620_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET2763927.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            complement(3144..4160)
                     /locus_tag="VQ620_001309"
     CDS             complement(3144..4160)
                     /locus_tag="VQ620_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET2763928.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLDFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            complement(4108..5049)
                     /locus_tag="VQ620_001310"
     CDS             complement(4108..5049)
                     /locus_tag="VQ620_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HET2763929.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            complement(5125..5490)
                     /locus_tag="VQ620_001311"
     CDS             complement(5125..5490)
                     /locus_tag="VQ620_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HET2763930.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            complement(5641..6699)
                     /locus_tag="VQ620_001312"
     CDS             complement(5641..6699)
                     /locus_tag="VQ620_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET2763931.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            complement(6862..8013)
                     /gene="nagA"
                     /locus_tag="VQ620_001313"
     CDS             complement(6862..8013)
                     /gene="nagA"
                     /locus_tag="VQ620_001313"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET2763932.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            complement(8166..9983)
                     /locus_tag="VQ620_001314"
     CDS             complement(8166..9983)
                     /locus_tag="VQ620_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HET2763933.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMIFLLSATAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            complement(10084..11226)
                     /gene="tgt"
                     /locus_tag="VQ620_001315"
     CDS             complement(10084..11226)
                     /gene="tgt"
                     /locus_tag="VQ620_001315"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET2763934.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMLLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFRKYFVEKYGYNKSGRNF"
     gene            11356..12213
                     /locus_tag="VQ620_001316"
     CDS             11356..12213
                     /locus_tag="VQ620_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HET2763935.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            complement(12241..12885)
                     /gene="pcp"
                     /locus_tag="VQ620_001317"
     CDS             complement(12241..12885)
                     /gene="pcp"
                     /locus_tag="VQ620_001317"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET2763936.1"
                     /translation="MKVLVTGFEPFGGEKGNPTLEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSVQVLEEEMNRYQPDFVLCIGQAGGRTSVTPEREAINQDDARISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRPTTPTMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(12992..13360)
                     /locus_tag="VQ620_001318"
     CDS             complement(12992..13360)
                     /locus_tag="VQ620_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HET2763937.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATRSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLWYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            complement(13371..13796)
                     /locus_tag="VQ620_001319"
     CDS             complement(13371..13796)
                     /locus_tag="VQ620_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET2763938.1"
                     /translation="MIEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIKVKEEMESILTEFERTELSRLLNKLVLGIENIEI"
     gene            complement(14221..15363)
                     /locus_tag="VQ620_001320"
     CDS             complement(14221..15363)
                     /locus_tag="VQ620_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET2763939.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQGTTPAEKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            complement(15529..16149)
                     /locus_tag="VQ620_001321"
     CDS             complement(15529..16149)
                     /locus_tag="VQ620_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2763940.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQNLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNTFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            complement(16153..17433)
                     /locus_tag="VQ620_001322"
     CDS             complement(16153..17433)
                     /locus_tag="VQ620_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET2763941.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKLTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            complement(17494..18978)
                     /locus_tag="VQ620_001323"
     CDS             complement(17494..18978)
                     /locus_tag="VQ620_001323"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET2763942.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTAELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVYESDS
                     YIEDPHTAVASAVYRKYQAVTGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            complement(19054..20331)
                     /locus_tag="VQ620_001324"
     CDS             complement(19054..20331)
                     /locus_tag="VQ620_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HET2763943.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIETAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            complement(20438..20521)
                     /locus_tag="VQ620_001325"
     tRNA            complement(20438..20521)
                     /locus_tag="VQ620_001325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20485..20487),aa:Leu,seq:caa)
     gene            complement(20531..20602)
                     /locus_tag="VQ620_001326"
     tRNA            complement(20531..20602)
                     /locus_tag="VQ620_001326"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20568..20570),aa:Gln,seq:ttg)
     gene            complement(20616..20688)
                     /locus_tag="VQ620_001327"
     tRNA            complement(20616..20688)
                     /locus_tag="VQ620_001327"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20653..20655),aa:His,seq:gtg)
     gene            complement(20703..20773)
                     /locus_tag="VQ620_001328"
     tRNA            complement(20703..20773)
                     /locus_tag="VQ620_001328"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20739..20741),aa:Trp,seq:cca)
     gene            complement(20778..20858)
                     /locus_tag="VQ620_001329"
     tRNA            complement(20778..20858)
                     /locus_tag="VQ620_001329"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20822..20824),aa:Tyr,seq:gta)
     gene            complement(20871..20943)
                     /locus_tag="VQ620_001330"
     tRNA            complement(20871..20943)
                     /locus_tag="VQ620_001330"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20908..20910),aa:Phe,seq:gaa)
     gene            complement(20947..21020)
                     /locus_tag="VQ620_001331"
     tRNA            complement(20947..21020)
                     /locus_tag="VQ620_001331"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20984..20986),aa:Met,seq:cat)
BASE COUNT         6302 a         4804 c         3732 g         6260 t
ORIGIN      
        1 cttcggatta cgaatgaatt tgattgttgg tattatcaat catgaactag gattctagtt
       61 ttgcaggaag tctaataatg aaaataaagg actaaagcaa taactctaaa tacatctgat
      121 ttttatagga tatttcataa aaaaaaatct acatacctag agtcctattt agttatgtat
      181 cttacataaa gagaaaggaa ttaattatca tgaaattatc tttgtatagt gaagttgaac
      241 attaccattt tttaagttct tattctttaa atgatattac atatactgat accccaaaaa
      301 atattattga aaagtctaaa aaagataagt atagataccc aatagtaata cttaataaag
      361 accataaagt aattggattt ttttgcctac acctaaacac tggtcctaaa gagtatggct
      421 attatcaaaa tgattatgcg cttattagag gattttctat tcatgataat ttcagaaatc
      481 aaggatatgg ttctaatgct ctgaatgaaa tatttgaatt catagactca actttaaaat
      541 tagaaattaa ccatattata ttggctgtaa atgaaaaaaa tatattggct caaaaagcat
      601 atagaaattc cggtttttct gttgtgaaaa aagatttaga aggaaaaagg gggaaattaa
      661 ttattatgga gaaaagcaga aactagtaat gatactagtt tctgcttttc tctaagtctg
      721 ctataaattt ataacatgtt gaaataagat gtgaataaat tgattgcaga attcaaacca
      781 atttctaaca atgttttaga aaactcggcg gactattcca gattcaatcc actatattct
      841 atcttatttg catattttga gagaaataat ctatatacta aacttttatg tttataattc
      901 attcctttcg tttcactcaa ggcacaagac agaatgaaaa agtggtgtgc tctttatttt
      961 gttttataat aatagtgaga aaacctatca ctactacaaa tcacggggag gtgaataagt
     1021 gagtggtata gccactacct cgcattttta taggtgtcat tctttccatc aagcacaaga
     1081 actgtgacta ggagcacgta aacttataat tgaataagcg actcgtttgt gaaaggaaag
     1141 aatagtcaag ggataggcag actcaaattt ggtgaaggga aacccctcat gttaaaagcc
     1201 aaacttcaat tttaaaaatt ttaaaatttg tttcgtattc actactcctt gcctttatat
     1261 tggattgatt aagagaacct ttcgcgtttc tagaaactac agtggactat tctggattca
     1321 acgcattata gttgaattga ctaggaattc tctttctttt caggcttctc ttctgatttt
     1381 tttctccgct ttttcgcttg aatcagtcgc tacctcttcc tttttatccg ttttctcttc
     1441 tttgattttg actgaaggaa acagaaactc atattgactc ttatccgtac gaacagtcgt
     1501 caccaagctt gttttcttgt tttgatagct cacctgacct agattaaaaa cctgttcccc
     1561 actttcttgc tcggctttat ccttggttcg tttagccatg gcaaaagcaa ccaatttttc
     1621 tttatttaaa gcatagtctt gatagtgggc gatttgtcgg gtcaaataaa attgcaacaa
     1681 aagactaaag atggctgcta tggtgactgc gtagaggaga acacctgcct taactttctt
     1741 ctttttccac acgatagatg aactcccttt ctaagccttt ttgaaactgg aaacgaaaat
     1801 gaaccagttg attgtcctct gtaatctgag ctgatttgag gccataaacc ataggctgat
     1861 acccccgtcc actagcatcg gttttacgaa aatcgtctga ctttgactta ccgatggcga
     1921 tgtccttgcc atcttgcttc atgtataggc gattgccttc tactttttcg aactgcgaac
     1981 ggtctaattc tgcctcaagt tggcccacaa acaagagcca ctccttttgc tcgctttgtt
     2041 gctggtagcg aacttctgaa atgaggagct gactcatagc ttgaaaaagg agtaatcccc
     2101 cactgatgac aatgagggca agcagggatt ctaacaaggt aaaagccttg accttatggc
     2161 tctttgattg ccaacaactg ttctgaacca tggtagacct ccaatccctt ttcactagaa
     2221 aatacctgaa tctcaactcc gttgatgctt acctgatttt gccccgtctg caaggccatc
     2281 ttagctaccc tcaagacttc ttccttttgc aagatttttg cttcctcttg cctatttttt
     2341 tgaatttgtc ccaaaaggag ggtcgcaatg ctggcaaaga tagctagagc gactactgct
     2401 tccagtaaaa tcactgccct aattttttgt ttccttaatg cgtttaattt ttccatttcc
     2461 tagatataat tgatagcgaa tcgctccttt actggtctga aattcaacct tagccaggga
     2521 cgaattgccc ccagctcggt caaatgtaat actttggcct gatggtgcct gaattccttt
     2581 aggaactgtc aacttttgac tgccattgct aatcatctgc ccatccaagt tcagactagt
     2641 cttttgttga ctagctacac tgcgtttttg ggtttcccga tagagttctt caaactccat
     2701 aaagaaaatc tgttcctcta ccgctgcaaa agtggactgg acagagccgg acaagcccaa
     2761 ggcaaggata ctcacaagtc ccaaaaccaa gagactttcc agcatggtaa aggccttaat
     2821 cattgacttt acgatttgcc cctccatttt tagtatagta ttcattataa gctttaacct
     2881 gttcttccgt gatgcgccca tctccttgta acttgctcag agtagcatct tcatttttat
     2941 ccaagctata aagttctgcc tggctttcca ccaccttaac aacagctgct tttcctttgt
     3001 cattgactgc ttctttttgc ttggtcagat taggtacaaa gagcaagaaa agcacgctga
     3061 taatcagcaa gaccaccaac atctccacca atgtaaaagc tttaacctta gcttttttca
     3121 agaatgtcat catttttttc attttaaaaa tttacctcca tattttgata catgggcatg
     3181 agcattgccg cataaagtaa aacgataatc agtgccacaa agataaaaac cagtggctgc
     3241 accaaattca tggtgcggtt gactcgggta aaaaaggctt cccaagtttt ttcagcatag
     3301 atttccaact cactacccag cttggactta acttccccat actctatgat gagactcaat
     3361 tccttcctaa agaaaggata ggttcctatc gtctgagaaa attcacggcc atttttcagg
     3421 gtttgagcca gatcttgacc gatttcttta aagagctggg aaccttgttc ctgcatcatt
     3481 tgaaaaatct gcgtcagctc cattccctgt gaaatcatat tcccccattc acgtgcataa
     3541 taggctgtca agtaggtctg cacaaagatt ccaataaagg gaaggcgtgc taagatagaa
     3601 aagacactca tcttagaact tcttttataa aaagttagtg ctaaaagggc aagcacggaa
     3661 acaagcccta ccatgcctag aaaaatttgt ggcagattac cgataattcg ggtggcaata
     3721 ttgctactat ccagttgtgg gagcaggtaa ttccgtagcc ccagcataat taagagaaga
     3781 aaatccagca aaatcaaagg ataggtcgct acttcaatta actttttctt gaccttggcc
     3841 agattgtcta gatattcttc tatctttccc aaactcaggt gaagattgcc atgaacttca
     3901 gctagagata actgagtgac aatggtactt gaacatccca aactttccat catttctgag
     3961 aatgatttcc cctgagacaa gcctgtacgc atctgggtca cacactgctt gtccaacaag
     4021 gaactcctat ctaaaaagga gatagtctcc accagatgaa aaccgctaga aaagagattg
     4081 ttaaataggg tgatgatatt tttttgctta gctgtagcta attttttccg tctcagcctg
     4141 aagacttgtg atatgtccat ctttaagaag ttggtcaatt tgctcattcc acttggctgc
     4201 ttggtgttct tgataatctc tgtttgcaaa gtcaacgatt cctcctcccc cgattaatct
     4261 ctggtagcag actccttgca gaacaactgc caattcttct tcactcacac ccaactccag
     4321 cagacgctca taaacacctc ggatactctt ggcgtgaatg gttgaaaaga ctgtcgcacc
     4381 tgtcaaacta gctctgacca ctgcacgcgc cgtctcgctg tcacgaattt ctccgataat
     4441 caagagatct ggtcgatgac gcaaggaaag tttgattaga ttttcatagg ttaggccgat
     4501 tgcttcgttc aactgcaact gaagcatgtc gtcctgcttg atttcgacag gatcttcgat
     4561 ggacataact tgctgtcctt taaagagtga cttggacaat tcatgcatca aggtcgtctt
     4621 accactccca accgggccag caaaaagata gagtccccgt tgcctgtact gcttgcctaa
     4681 ttcttcaata tcctgaaacc aaaaatgcaa gtcctgctcc tcatcgtgca acaaacggat
     4741 aaccaaactc tcatgccccc gataatcgcc tacagtagat aaacgtagag acgccatctt
     4801 ctggtcatag gcataatcac aggaacccag ttgactacgt cttttttctc ccacattcat
     4861 acccgccaca aacttaaagt gactgataac ggctgcaaac ttttcaaaat catagctacc
     4921 aattttacag cgctcgtctc ctaccctcat atgaagctca taggcgtcta acttagggac
     4981 aaaataaata tcctgcgtcc cttttttccg agctgaacga atgatttctt gtgcaatttc
     5041 ttgaaccata cttacctcct cacctatact attcgcaaag cgttagaaaa ataaaaaaag
     5101 caactccaaa aaagttactt ttctctattt taatttcata cggcaagaac gattcctttc
     5161 ctgactggtt tgtttttcat agcctgagcg tagtttttcg tctgggataa cctgctcatc
     5221 ctgcaaaaga gccaaagaat ggtattgttg taaatactca tcacattcat cacaccagcg
     5281 ttggtcttcc gggtcccaaa aaatggtcat caagtcgctt ctacttttat aaagagggga
     5341 ttcttgttcc aatctaaacc agcaagtttt gtagtatttg agagcatcat aatactggtc
     5401 aaattttcta cttgctacaa tatcttcttc ccaaccttct aagaaccacc acggttcaaa
     5461 atctccgtac atttctataa cacgatacat attcattcat tcctttgtac cgtatattat
     5521 aaccaattcc gccagacaag gaaagaaata tgctcatttc ttaatttttt gataaaaaat
     5581 ccagccatct gatggatgac tgaatttcta tttgcttatg tacaaaaata tttttactac
     5641 ttaggcttct gagatatcgt tttcgataat gaccttaatg gcatggtggt ctgctgcctt
     5701 actgaagact tcgtaggctt tttcaatttc actgagtttg aaatagtgag ttaccaattt
     5761 ttccggttca atcttatgac tttcaagtgc tttcaacaat tgtggagtcg tatttgtaga
     5821 taccaaacca gttgttacat tgatgttgcg aatccaaagt ttatctaaat cgaattcaac
     5881 tggtttacca tgcacaccac agttggcaac cgttccgtct acaccgataa tcttttgaca
     5941 gaaatcaaat gtttcaggaa tccctacagc ttcgatagcg acatccacac cacgaccatc
     6001 tgtcaaatca taaatttctt taatggcttt tttagggtct gaagaattaa ccttatgagt
     6061 cgcaccgaat gatagggcag tttccaagcg gttatcgtct aggtctacca taatcaattt
     6121 agctggtgaa tagaattggg ctgttaaaag agcagccaat ccaactggac ctgaaccaat
     6181 aatggctacg ctgcaaccag gttctacttt cccttttaag acaccaattt catatccagt
     6241 aggcagaatg tctgacagca taaccaaagc ttcatctgac aagtcttctg gagtatggta
     6301 aagagtatta tctgcatgag ggacacgtag atattcagcc tgcataccat caatcaagtg
     6361 accgaaaatc cagccccctt cgtcttcaca gtgggcataa attccttttt tacagtagta
     6421 gcatttacca caggcacaga cgcaagaaat caagaccttg tcaccttttt tgaagttgga
     6481 aactccttcc ccaacttctt caacaatccc aatcccttcg tggccaagaa tggtaccact
     6541 ttggcaagta ggaacatccc ctttgataat atggaggtct gttccacaaa tagtggtttt
     6601 tacaatacgc acaatagcgt ctgttggctt gcgaataact ggtttgtcta catcaacaaa
     6661 agaagcaagt cctggtttaa cataagtata ggctttcata aagcactcct ccgttttttt
     6721 atttgtaata tttatagttc aattgtaagc ctttttcttt gtttcttcaa gctttttttg
     6781 tgattttttt aactttttat ttaccagtaa agtaccctgt ataaaaaagc agaagctagt
     6841 ctctaacttc tgccttctct cttatgcttg ataacgtttc acaccatcta ggtaggttgc
     6901 taccaattcc aaatctttat ctaatacgat gaagtcagcg tcgtagcctt cacggatttg
     6961 gccacagacg tcatcgatgt gaacggattt agctgggttg aagctggcca tcatgactgc
     7021 ttcatgcgga ttcgcaattc cccattcgac cacattcttc aaaccatctt tgagtttgag
     7081 gatagaacct gccaagttac ctgtcgattt gaggcgtgca gttccatttg caacgacaac
     7141 tgggaattct cccaacatat aatctccgtc ttccaatccc ccagctgtca tacagtctgt
     7201 gataagagcg atgttttctg ttcctttttg tttgataaga atttcgcaag cctttggatc
     7261 tacgtggtga ccatcacaga tcaactctgc ataggtatgt ggcaattggt acatggctcc
     7321 aaccataccc aattcacggt gagtcaaccc acgcattcca ttgtaggcat gcacccaaac
     7381 actcgctcca gcatcgactg cttttttggc ttcatcaaaa gtcgcgtttg aatgtccaag
     7441 agcaaccgtc acaccttcgc ccgtaactgt acgaacaaag tcttccaccc catcacgttc
     7501 tggtgcaagg gcaattttat taagcaagcc atttgccgct ttttgccaag aatgaaactc
     7561 ctcaacaccc gggtctctca tataagttgg attttgtgcc cccttaaaag tttctgtgaa
     7621 atatggacct tcataataaa tcccacgaat cttagcacct gttgcttctt tataatggtt
     7681 tccaagattt tcagtgactg caagcaattg ctcataagtg gctgttaaag ttgtgggtaa
     7741 gaaactggta acaccggtac taagaagtcc ttcactcata gtatgcaatg taccttcaat
     7801 gttgttgtcc atcacatcta cacctgcata tccatgaata tgagtatcca caagtcctgg
     7861 ggcaatgcta taacctgtat agtcaatcac ctcagcccct tcaggaatct gctctacatg
     7921 tttcccaaac ttgccgtcca caagttccaa gtaaccacct cgacgaactc cgtgtgggta
     7981 gaaaaactga tccgctttaa tatagttagg cataatgtta acctccttaa aagattgatt
     8041 ctacaattta ttatgtcaat tacattataa acctttcttt tttatttgta aatggtatag
     8101 acctattttt tggagatatt ttaaaagagc tggatttctc caactcttct cttttttaat
     8161 acaagttatt ttgatttaac cggcttgtct tgagctgtct gcaaagctgt ggcaatcgta
     8221 tctgcataca attttgctcc tgcttcgata gtgctactct cactcccgaa atgaacctgg
     8281 tctgttccag cccaaatttc tggatgctct ttcgcaactt gattccaatc tgctatcgta
     8341 atgtaaggtg tcttctctgc caattctctc atataggcag cagccttctc aacgatggca
     8401 taggtctctt ttgtcttatc tccctcataa ggagtcacca aaatcatatg gtgtccctta
     8461 ggaagatttt tcacgatact gtcccagtca tccttgtaat tctcaggatt atttacccca
     8521 gtcgcaatga ccaccgtctt aggtaaaaat ttattctggc tattatttag catgatttca
     8581 tttgcggtct tggttgttac gctgacctgc gcgttaatct gtgctccagg aagagctgtc
     8641 tgtagtgctg tatttgccct taaagccact gagtcaccaa ttaacatagt gccatcagca
     8701 attcccaaac tgtttgcatc tgcccgttct gccatcacct tggtctggcc aatatttgtt
     8761 gcagcttgct tcaagccatt gacagtcaag tctgtctcaa acgctcccac ttgtggtgcc
     8821 aacaaggtca ccatgcagac aatgaaggtc aagattcctg tacctgctgc aagaattgcg
     8881 tgaatataag gcaggggacg aagagtttgt aaaataggtg tgttcttgcc tgcaatccaa
     8941 ggttccaata cataaaatga cagactggca aagccataag aacaaatcag agtcagtaat
     9001 acagcaagaa gatttgatgt caactgtgag aaaatgatat agaaaggcca atggaaaaga
     9061 taaaccgcat agctagtatc cgctaaaaag ctgataatct tcggctcctg tatatgatgt
     9121 gtcttttcat gtaagacacg cgccgccaga atcatggcaa gagctgcaag actggcaagc
     9181 aagaagccga taagataggc aaaaagataa gtgaatttga caaagaaagt caaaagaact
     9241 aggaaaccaa agcctcctcc aaaaactact aaagtctttc gtaaatccca gattttatcc
     9301 aactgcttga cgagggaagt cgtctgacga acgcctacaa tagttgctaa catacttccc
     9361 aaaaagaatg gatagacatg agttaaactg gagaaataaa cagaggaata agaggtcact
     9421 agaaaactac caataaacat ggagaagaaa ctgatcaaga aggcagtagc agataagaga
     9481 aagatcatcc ccttcaactg accatttgat ttagcttgtt tggataagaa ccaaactgcc
     9541 aatccccaaa gaatatagta gtgaacctca actgccaaac tccaattatg aacaaacaaa
     9601 tgaggaatga actgagattc ataactccca cctgttaaga gttcatagaa gttggtcata
     9661 aagcctaaga cgcccgcaat ctggccacca attccagcaa catagtcttg gcgaaccaag
     9721 aaagtaaaag gcatggtcac caagaccatc aaaaccacag gtggcacaat ccgataaaag
     9781 cgtcttctaa aaaatcctat caaatcaatc tcattgttct tagaaaattc ttcaatgagt
     9841 agagctgtaa tcaggaaacc tgaaaatgtg aaaaagacat ctaccccgaa aaatcctcca
     9901 ggaaagatcg tctgaaagaa gtgatacaag agtaccaaaa gtaaacctat aatcctaatc
     9961 aaggaaaacc atttaatgcg catacgagtt tattcttcct ttcattgtac actttattct
    10021 atcaaaaaaa gaagaaaaat cctaagagaa aacttaggat ttttagctta tatcaattcc
    10081 attttagaaa ttacgtcctg acttattata gccatatttt tccacaaaat acttacggaa
    10141 ttccaagaga ttgtcatcca tgatggcttg tcgcacttgc ttcatcaggt taagcaagaa
    10201 gtaaagattg tggtagctag tcaagcggat accaaaggtt tcatcagcct tgagcaggtg
    10261 acgaaggtaa gcgcgtgtat agttattaca tgtgtagcaa tcacactcag gatccagtgg
    10321 cgtaaagtcc tcagcaaact gggcattttt cacaaccaaa cgtccttgac tggtcataca
    10381 agtcccgtta cgagcaattc gagtcggtaa gacacagtca aacatatcca ccccacgaat
    10441 gaccccatcg atcaagctat ctggcgctcc cacacccatc agataacgag gtttattttc
    10501 aggcagcagt tgagttgtaa agtccaagac cgcattcatc tcttcatggg tttctcccac
    10561 tgccaaacca ccgatagagt agcctgagaa atccatgctg acaagatcgt gagctgattg
    10621 gcggcgaagg tcttcaaatc ctgcaccttg cacaattcca aacaaacctt ggtcatgtgg
    10681 acgacggtga gccttcaaac cacgctcagc ccaacggctg gtacgctcga tcgatttctt
    10741 aacgtagtca taaggttgat aaaactgagg acattcatca aaggacatca tgatgtctga
    10801 acccagatta ttctgaatag agatggcttt ttctggggat aggagcatct tagaaccatt
    10861 tagatgattt ttaaaggtta ctccttcttc tgtgatatta cggctatctg ctaaagaata
    10921 aacctgaaaa ccaccactat ctgtcaagat aggctggtcc caattcatga acttgtggag
    10981 accaccagcg cgtgcaatga gttcatctcc agggcgaagc cagagatgat aggtgtttga
    11041 taggataatt cccgaaccca tctccttcaa ttcttcaggt gactgagttt tgacagtggc
    11101 ttgtgtccca actggcataa acataggtgt cggaaaggta ccgtggggag tgatgatttc
    11161 tcctagacga gctcctgtgt gtttttcttt cttaatcaaa cgatatttga ttggtgaatc
    11221 tgacattttt tacctccgaa gctgggaaag acagtcccag ttcatacagt gtgcccaagg
    11281 acacactgta tgattttatc aaaaaaagta ggaactgtca cgaactatgt ataagaacta
    11341 tggtataata agagaatgaa ctatccaaaa attgatttaa aaacaattcg tcaggagtca
    11401 aaacattttc aggctgacac cccccgtctc tttctcctct atatccttcc cagtatgctg
    11461 gtcatcttat ctggttttct taatccctta tctcggatcc acgggactgt tttagaacag
    11521 ccatttttca gcatacttgg ccaaatactt caaacctacc tctttcctct actagtctcc
    11581 tttatcggga ctattctttt gaccagttcc gtctatgcaa cactgacact catgaaagat
    11641 tctaaaacgg agccatctgt aaaaaacagc ctagctctct ttgatgagga gcgcttttct
    11701 caaacctttt tgaccctact tctcaaacgt ttctatctct ttttatggag tatccctaat
    11761 ttacttggga tttacctcct cttttatagt agctttttag ccaaaaaatt cgtcacccta
    11821 catcctgagt ttccaaatct agatctctca tcagttgaaa ccgagcgttt cctcatggtc
    11881 tttggactct actttcttgc aagtctcatc ttgatcattg taggaaatat cctctatatt
    11941 ccacagtatt atgcctattc acaggtggaa tttcttctct gttatagtct agacctagga
    12001 caggtcccac caagacgaat cctaaaaact agccgttcct ttatgaaggg ctacaagttc
    12061 cagcactttg tcctagattt acagcttctt ccttggtact tcctcaattg gattaccttt
    12121 ggcattgcta gtttttcact cctaccttat atccaatgca ctaaaattat gttttaccga
    12181 gcagtattgg ctcgaaaacg tccaaaagct tgaaggtttt tcccctcaag cttttttcta
    12241 tcaatgagtt tctccgccta ccaacttgag ttcctgatct ccatgttcta tcatagcgcc
    12301 gattgctgct tctatccctc gccgaatatc cactaaactc atagttggag tagtcggtct
    12361 gttcaccacc tgttccatca tataaggaat atgcataaaa cctgccttaa catatggaaa
    12421 tttcttttct accaaataga gagcctgata catcaaatgg ctgcagacaa aagtccctgc
    12481 cgtattggaa acagaggccg gtaagccctc tttttttata gcttgaacca tcgctttaat
    12541 cggcaaacta ctaaagtagg ccgaagcacc atctgggcga atgggacggt caatcggttg
    12601 attatcttcg ttatcagaaa tgcgtgcatc gtcttgatta atggcctctc gttcaggtgt
    12661 cacactagtt cttccaccag cttgcccaat acaaaggaca aagtcaggtt gataacgatt
    12721 catctcttct tccaatactt gaacagattt gtgaaaaact gtcggcacct ctagccaacg
    12781 gacctcagca ccatggattt cagctggtaa acctttaatg gcctccaaag ttggattgcc
    12841 cttttcccct ccaaagggct caaaacctgt cactaatact ttcatttcta actcctcaaa
    12901 accaattcaa tgagactttt ttcttaaaaa caagtataac atatttccct tattctggat
    12961 taaaaaggat tagcgagatt aaaatcgacc ttcaagtgta tttaaagagt aaaatactaa
    13021 tcaaggccaa aatagctgat ccaccttgct tcaaaataat ttttttatcc gctgttaaag
    13081 agccgtaagt cgcagcacca atcacaaata agacaaaaat agtcacaatt tctaaattct
    13141 gtgagaaata aatgccatac cagagaaaga ctcctagcag agccttataa atcccttgat
    13201 ttttgaacaa tgaacttact gacggatgag ccaattcttc cttttccata ttaaatacac
    13261 gactagtcgc atctgatcgc gtagcaatac tttccaaata aaaaatgtaa aaatgctcca
    13321 aagcaacgat cgttgttaaa ataattgtaa taattgacat ctatttctcc ttaaatttct
    13381 atattttcaa tacccaaaac caatttattt aataaacggc tgagttctgt tctctcaaac
    13441 tctgttaaga tactttccat ctcttcctta accttgatat gttgcagagg gggattcacc
    13501 actaactgct ccttggcata cttcgcagcc tctaccaaca cttcccgctg attttcagga
    13561 ttacgatgac gctccaccaa accttccgtt tccaaaatct tgaaatgccg tgtcaaagca
    13621 gcctgatcaa ttttcaaacg ctcctgaacc gccatttggt tacaaggaga atgctccagc
    13681 aaaaacagta aaatctgata ccgtgtcaaa ctaatcccaa gccttttttc aaacaattgc
    13741 gtactcgctt gctcagacaa gcggagttga tacagtaaat cttgaatctc tatcatttct
    13801 tcctcctatc cattgactta tcaatagttg accaatcaat tataatataa agtatagtga
    13861 aatgaaataa gagcaggaca aatcgatcag gacagtcaaa tcgatttcta acaatgtttt
    13921 agaagcagtt gtgtactatt ctagtttcaa tctactataa aatagattgc aagaatgttg
    13981 cttagattat tacaagatga taactttcca ttttccattc aaattgatac tcaatgaaaa
    14041 tcaaaaagca aactaggaag ctagtcgcag gttgctcaaa gcacagcttt gaggtttcag
    14101 ataaaactga cgtggtttga agagattttc gaagagtatg acaaagaaaa aacgaacgag
    14161 aatcgcttcc catcacgaaa gctagatctc attcgtcaaa atgatgttac caagtcgggt
    14221 ttatccattc attgaaacgt gaacgtgatc atagtgattt tctgtcacac taccacggtc
    14281 tggcattggg ttccaagtgt tagctggccc atatttgcta tcgaatggag catagaaacg
    14341 ttgtttccag atgatgtaac taatgccacg gctggccatg ttttgaatag catattccgc
    14401 aatcttatcc cctaattctg aacgttctgg taccataaag tcgatagcca aaccttttcc
    14461 gtgatctcca ctgtctcctg gacgataacc gctaaaggat gtaatgccaa acaagttagc
    14521 aatttcttct ttaaaggcag ctgtttgtgg ttgaagacct gcattttcag attttgctac
    14581 tgcaagtcca gcataatcgg gcgcagctgg agcctcgtaa gttgttgaaa ttattttcgt
    14641 ctcttctggt tgataagtag aaactgctgc tgtagctcca tttgatgatg ctacttcttt
    14701 tgcttctgaa cttgttgtag ttgcttccac tgctgatgca gcttgagggc ttgcttgtgt
    14761 ttcctgcttc tcagctggag tcgttccctg aggagcttct tctgcaactg cactgcttgt
    14821 ttcggccgtt tgctcctctg ggacagaagt gcccgtagat ggtgccactt cttcagaagc
    14881 aatcactgtc tttgtaactt ctgaacttga tgctacttct tttggagctt ctgcaattgg
    14941 ttgagaaagg tctgcaacct gaacagtttg atcatcaacg gtcacttgat tagtggtcaa
    15001 atctgctgtc gcagttgtca cttcttcact agagtctgct tgaggtgttt ggatttcaac
    15061 ttctgttact tcttctgctt cattgacagt cgttgtcaaa acagtttctg ggaaaatcaa
    15121 gtccatatta gtgattttgt tcagattcgc aagcactgtg acatctacac ccaaggcttc
    15181 tgcaatggtg ctcaaagtat caccatactg tacggtataa cttgttttgt tgtccgtttt
    15241 agtcaaatcg ttttggattt gctcaacact acgtgcagtc caaagaactt cttctgcttg
    15301 agttgccaat actggggcaa atgacaaggc tactgttgac gctaataaca ttcttttctt
    15361 cattctttca aattcctttc aaatgagtac ctgtctatca taacacaaaa aatgtagaaa
    15421 aaaagccctc tcgggcctat ttaacagaac tgtctattcc ttgtaacaaa ctagattact
    15481 tgaaatagtt tgttaggtct ctgtcacaaa actgacacaa tctttttatc acttagtctc
    15541 aaaaatacgg gaaatatctg ctaacgcttg aatcctgtga ttcccttcat aagtagactc
    15601 taaaaagttg atactttgaa tcccactatt ctgggcaaat tccacatcca gagtccgatc
    15661 ccctatataa taagtattat cagaattcaa ctgatactta tctagcagat aggtagccgc
    15721 ttctggactt ggcttccgca caaagccact ctgactggtc aaaatctctg taaaatagga
    15781 ttccaccccc aagtccttga gaatggtaaa agtgttgttc cccttatgag tatatataaa
    15841 ctgctgaatt cctgattcgt ctgcccaagc tagcacctca cgcgcacctg gcatcaaaac
    15901 tacctgagca ttcttctcag ccagactctg ggcacgcacc tgatttagca cctcaacatc
    15961 cagatttcta tcttctgcca cccgcacaag caaattttgc accgaatact tgaagataaa
    16021 ctctctcacc ttctccttat cataaggaat agaaaactga gcaaaagtct cctcaatccc
    16081 tgataaaatc gcttcgtaag agtccaataa agtcccgtct aaatcccaaa taaaagctgt
    16141 tttttgcatt tcctatcctt tcaagctcat atagcgctgg taacgatagc gtagaaataa
    16201 ccagcgaaaa ccattatcca agagagaccc tgcccaaata ccaggcaagc cccaaccaag
    16261 cacaataccc atcagatatc ctgtcccaat gcggatacac cacatgccta tacttgtcgc
    16321 ataaaaaggg aggcgtgcat ttcctaatcc ctgccagact gccgtataga tgactgttcc
    16381 tgtcgtcata ggggtaccaa gtagtgaaaa cagtgtcact agaacactag cctccaccgc
    16441 tagagaatca gtcgtataga gatgagttaa tggtacaccc aagacatata tactaaagga
    16501 caggggcaac atgaggaaca gagaaagcca aaaggtttgt ttactcaaac tagcaactct
    16561 tttccaatca tcctctccaa ctgctcgggc caacagcatg accgttgccg tagcgacacc
    16621 aaaggcaggc atatagttaa actgggtcaa gacttctccg attgcattcc cagcaactgc
    16681 ctccgtccca aaagaaacga ccaaggcaat gatcactaca tctccagccc tcatcataag
    16741 tcgctctcca gctgctggta aagccaaggt caacagttcc ttatctaaac caaaagttag
    16801 cttcccataa ggcagtttta attgtgacca caaaatcaca agaccaacca aacgagacag
    16861 aattgtcccc caagcaacac cagctatccc catatccaga acaaaaatag ctagacttga
    16921 aaaaagaata ttcaaggcat tggataaaaa actaacatag agaggcagac gtggattatg
    16981 cgttgcacga atcaaggctc ccaaactagt cattaaacct aagagaacaa tcgatccgcc
    17041 taccaaagat agatacagtc caccactctc agctacatcc ctctccgtcc ccaaaagtcc
    17101 tatcatctct ttcccagcga agatggacaa aaatcctaaa aggaaactta atagtaaggt
    17161 aatcttcaac gcctcagtca catgataggc caacttcgac tggtctttct gccctatgct
    17221 ttttgaaata acactggaaa tagcagctcc cagagcgatg aaaatcgcct gataaatggt
    17281 gataatatta ccagctactg aaacccctga aatagctatc aatcctaaat gagcaaccaa
    17341 ataactgtcc accattccca ttagcatctg caaaaagttt tcacccatag ctggcaatgc
    17401 aatattaaga atgtctttat ttttcttaaa caatctctcc tcctgatgaa aagaaactca
    17461 gttggtttcc caaccgagtt tgctccctct gtcttaaagt cctaaataag cctcaaccgc
    17521 tgcttgcatg tcagcagctg ccactgttgt cttgtgacga attggagcta tttcaagccc
    17581 atcaactgct ggtggcactg caacgcctga gatttcatgt aattgagcca aggcttcaaa
    17641 gtctgtcaaa cctgcttttc cagttacagc ttctactgca actactggga acttgtatgg
    17701 actagctgtt gaagcaatca ctgtcttagt tacatctcca gtaaccgctt ggtattttct
    17761 ataaactgct gaggcaaccg ccgtatgtgg atcctcaata taagaatctg actcataaac
    17821 acgcttgatt tctgccgctg tttcttcctc agtcgcatat tcagctgcaa agaggtccaa
    17881 aatctctgca tcaaagtctg tcaacttata ttgtccttgc gtgttcaagg cattcataag
    17941 ttcagctgtc ttttcagcat tatttcccaa aagatggaaa atcaagcgct ccaagtttga
    18001 agataccaag atatccatag atgggctggt tgttacctta aactcacgtt ttttgtcata
    18061 gacacgtgtt ttaaagaagt ctgtcaagac attattgtca tttgaagcac agattagctt
    18121 gccaactggt aggccgattt gtttggcata aaaggcagcc aagatatttc caaagtttcc
    18181 tgttggtact gtgaagttaa ccttttcacc agctacaatt tcaccagtct taaccaattg
    18241 agcgtaagca taaacataat aaacaatttg tggcaccaga cgaccaatgt tcatagagtt
    18301 agctgatgaa aattgcaact tgttggtagt caatttttca cgaagagcca cgtcgttaaa
    18361 catgtgcttc acatttgttt gcgcatcgtc aaaattacca tcaatagcaa taacatgagt
    18421 attgtcacca ttctgagtgg tcatttgcaa ctcttgtacc ttgctgacac caccctttgg
    18481 ataaaagacg ataatctcag taccaggcac atccgcaaac cccgccatag cagctttccc
    18541 cgtgtcacca gatgtcgctg tcaagataac aatcttgttc tccaaaccat gtttcttagc
    18601 agcagtcgtc ataaagtatg gcaaaataga caaggccata tccttaaagg caatcgttga
    18661 accatggaaa agttccaaat tgtattgccc atctaatttc actaatggtg caatagctgg
    18721 agtatcaaat ttgctatcgt aggcattgtt gatacagtag tccaactcct cagctgtaaa
    18781 gtcatctaaa aatgctgaca aaactagctt agcaacttcc tggtaagaag catctttcaa
    18841 tttgtcaaag tccaaatcta cctttggata agtaaccggt gtaaacaaac cgccgtccgt
    18901 cgccaaacct tgcaaaattg cttggctggc agttactgta ttgttggcat cacgcgttga
    18961 ttgataaact aatgtcatta ctctctatcc tttatctata gtctcgtcca ttatatcatg
    19021 atttttcaga aaattctccc tttataagag aaattatagg ctcaattctt ctctcaaagg
    19081 ctgtaaatag gtcatttctt ccttatttct caccactagt ccctcctcag ctagtaggag
    19141 taagtctttg gaaaattcaa taatcgtagt ttcttcctca tctgtaagat ttttcttaga
    19201 aaattgtctc cttaaagact tgtaatcata accaaatact ttaaagaaag gtgctgtttc
    19261 taagtaagct tctaacttgt ctaaattaac caataatccc aagtgaaaag ctgcagaagc
    19321 aaaagtccta tcaagtggct gtgtacacac actacgaaac tcaactgttc ctcgagtcgt
    19381 taaatcttgg tactggtaac tacgatgagt ttcaaaatcc ttctcttggg ggtaaataat
    19441 aacctcatcc ccattcagag caaatgcttg gatttcggac gtagccaaat agtccccagc
    19501 ctgaatagga taaaaataat aggtctgccc atcacgttcc gcagtaaaaa tcgcagaatg
    19561 atttagatag tcaaaaaaat cagcttcatc attaaggagt ctagcattga ccccaacatt
    19621 ctctggatag ataccatgca tagattcttc ccagaaaata tcccttgaaa ttttcgtatc
    19681 ccaatccgca cccgaaaatt cagagtttgc aaataaataa gccttagccg tttcaatttg
    19741 agtaaaagca ttaatcaccc gtaagtagtt ggtttttgaa atatccagct gaacctggct
    19801 cccacagata aaagtaccat attcagggaa atgatgtaaa tctgatttaa taatatttct
    19861 actcaaattc aaataatcca tcaacatctg atagcgtgga taagccactg gacaattctc
    19921 atttttatcc cagttgggat ggataccaca gccaacaata gcatgatttg attcagctaa
    19981 ctttctctga attacattca tataattatt gaaacgattt tcgacctctt gaatcgtttc
    20041 agccttacca aatgcaaact caatcgttgt ataggaaact tcaaataaaa tagcatcctg
    20101 acttatcgga tctactaact ggatcagatt gccaaaatca tctacctttg ctacggtgag
    20161 atccaaagta gaaactaaat aatgaaatag atcctttata acttcaacat ctgtagcatc
    20221 cccttctaaa cttgcaacag gatactccaa ctcaattccg acaaacaatt caggattttc
    20281 tttaatattt ttcaaatacc gatgttttaa taaatcgata gaacaagaca tactacccta
    20341 cactttctta atctaaataa aatttgaata actataatta taccaaaaat aatataaaaa
    20401 aggaacgaag aataacaacg ttcctaacac tatactatac cggcggccgg ggtcgaaccg
    20461 gcacgtcctt gcggacactg gattttgagt ccagcgcgtc tgccaattcc gccacgccgg
    20521 caaatagtaa ctggggtagc tggattcgaa ccaacgcatg agggagtcaa agtcccttgc
    20581 cttaccgctt ggctataccc caataatata aaataggcga gtgatgggga tcgaacccac
    20641 gcatgccaga gccacaatct ggtgtgttaa ccacttcacc acacccgcca taattctatt
    20701 aacacgggca gtaggaattg aacccacact gaaggttttg gagaccttag ttctaccttt
    20761 aaactatgcc cgtaaaatgg aaggggaggg attcgaaccc ccgaacccga aggagcggat
    20821 ttacagtccg ccgcgtttag cctcttcgct acccttccaa aatatataaa tggcgcgaga
    20881 cggaatcgaa ccgccgacac atggagcttc aatccattgc tctaccaact gagctaccga
    20941 gccttattgc gggagcagga tttgaaccta cgaccttcgg gttatgagcc cgacgagcta
    21001 ccgagctgct ccatcccgcg ttaataatat aaaaggagga tgtgggattc gaacccacgc
    21061 acgcttttac acgcctgacg gttttcaaga ccgttccc
//
LOCUS       DAVBGY010000036        21054 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVBGY010000036 DAVBGY010000000
VERSION     DAVBGY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21054
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.036"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            204..2363
                     /gene="pfbA"
                     /locus_tag="VQ620_001332"
     CDS             204..2363
                     /gene="pfbA"
                     /locus_tag="VQ620_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HET2763944.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVISTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKESVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            2347..2643
                     /locus_tag="VQ620_001333"
     CDS             2347..2643
                     /locus_tag="VQ620_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763945.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYSKMK"
     gene            2885..3193
                     /locus_tag="VQ620_001334"
                     /pseudo
     CDS             2885..3193
                     /locus_tag="VQ620_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            3357..4016
                     /locus_tag="VQ620_001335"
     CDS             3357..4016
                     /locus_tag="VQ620_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HET2763946.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            4041..5108
                     /locus_tag="VQ620_001336"
     CDS             4041..5108
                     /locus_tag="VQ620_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2763947.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            5212..6222
                     /locus_tag="VQ620_001337"
     CDS             5212..6222
                     /locus_tag="VQ620_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2763948.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            6234..7925
                     /locus_tag="VQ620_001338"
     CDS             6234..7925
                     /locus_tag="VQ620_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2763949.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            7928..8638
                     /locus_tag="VQ620_001339"
     CDS             7928..8638
                     /locus_tag="VQ620_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET2763950.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            8734..9102
                     /locus_tag="VQ620_001340"
     CDS             8734..9102
                     /locus_tag="VQ620_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763951.1"
                     /translation="MKDYKINFDLGKIEYFDNNCLIQVYKFISFYDICEMVFAFHLPP
                     DELITNVIFKEKINSMLKCYIDRLLYVFINPTHFTEKVNLQFYGSFFSYEFICREVGN
                     ILKNKGVKCNLNFFEGEEYL"
     gene            9210..10211
                     /locus_tag="VQ620_001341"
     CDS             9210..10211
                     /locus_tag="VQ620_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2763952.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            10816..10893
                     /locus_tag="VQ620_001342"
                     /pseudo
     CDS             10816..10893
                     /locus_tag="VQ620_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            11394..12755
                     /gene="trpE"
                     /locus_tag="VQ620_001343"
     CDS             11394..12755
                     /gene="trpE"
                     /locus_tag="VQ620_001343"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET2763953.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQAIDRDPLDFLYEVTHKSQHHSDLPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRSVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            12752..13318
                     /locus_tag="VQ620_001344"
     CDS             12752..13318
                     /locus_tag="VQ620_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET2763954.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            13329..14333
                     /gene="trpD"
                     /locus_tag="VQ620_001345"
     CDS             13329..14333
                     /gene="trpD"
                     /locus_tag="VQ620_001345"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET2763955.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEDYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            14330..15097
                     /gene="trpC"
                     /locus_tag="VQ620_001346"
     CDS             14330..15097
                     /gene="trpC"
                     /locus_tag="VQ620_001346"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET2763956.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDC
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNVGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            15084..15683
                     /locus_tag="VQ620_001347"
     CDS             15084..15683
                     /locus_tag="VQ620_001347"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET2763957.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            15661..16884
                     /gene="trpB"
                     /locus_tag="VQ620_001348"
     CDS             15661..16884
                     /gene="trpB"
                     /locus_tag="VQ620_001348"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET2763958.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            16877..17653
                     /locus_tag="VQ620_001349"
     CDS             16877..17653
                     /locus_tag="VQ620_001349"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET2763959.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(17625..17726)
                     /locus_tag="VQ620_001350"
                     /pseudo
     CDS             complement(17625..17726)
                     /locus_tag="VQ620_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            17955..18095
                     /locus_tag="VQ620_001351"
     CDS             17955..18095
                     /locus_tag="VQ620_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763960.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WT"
     gene            18132..18686
                     /locus_tag="VQ620_001352"
     CDS             18132..18686
                     /locus_tag="VQ620_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763961.1"
                     /translation="MFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFPL
                     IQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNSN
                     IEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDIP
                     NFSELSETEDFEIIPRIEKQEGQK"
     gene            18701..19852
                     /locus_tag="VQ620_001353"
     CDS             18701..19852
                     /locus_tag="VQ620_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET2763962.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGNRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(19904..20563)
                     /locus_tag="VQ620_001354"
     CDS             complement(19904..20563)
                     /locus_tag="VQ620_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET2763963.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACVIIFGKLLL
                     V"
     gene            20633..>21054
                     /locus_tag="VQ620_001355"
     CDS             20633..>21054
                     /locus_tag="VQ620_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2763964.1"
                     /translation="MTIRFEEKVSIENAQLVCQWSNSLGKVFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVR"
BASE COUNT         6991 a         3288 c         4452 g         6323 t
ORIGIN      
        1 tagtttcagt ctactataga actgaccaag tcagtaacct agacttaggg caaggcggca
       61 ctgacctagt ttgaagagat ttccgaagag tataaatttt aatattttct tgtgttattc
      121 cttgacaatt caatttggaa aatatatgat aaagataatg acagcggtgt cattctatct
      181 attttaagaa aagtaataat caattgttaa aaatagtaaa aaaattggag gttctgatga
      241 aatattttgt tcctaatgag gtattcagta ttcgtaaatt aaaggtgggg acttgctcgg
      301 tactattggc aatttcaatt ttgggaagcc aaggtatttt atcggatgaa gttgttacta
      361 gttcttcacc gatggctaca aaagagtctt ctaatgcaat cactaatgat ttagataatt
      421 caccaactgt taatcaaaat cgttctgctg aaatgattgc ctctaattca accactaatg
      481 gtttagataa ttcgttaagt gttaatagta tcagctctaa tggtactatt cgttccaatt
      541 cacaattaga caacagaaca gttgaatcta cagtaatatc tactaatgaa aataagagtt
      601 ataaggaaga tgttataagt gacagaatta tcaaaaaaga atttgaagat actgctttaa
      661 gtgtaaaaga ttatggtgcg gtaggtgatg ggattcatga tgatcgacaa gcaattcaag
      721 atgcaataga tgctgcagct caagggctag gtggaggaaa tgtatatttt cctgaaggaa
      781 cttatttagt aaaagaaatt gtttttttaa aaagtcatac acacttagaa ttgaatgaga
      841 aagctacaat tctaaatggt ataaatatta agaatcaccc ttccattgtt tttatgacag
      901 gtttatttac ggatgatggt gcgcaagtag aatggggccc aacagaagat attagttatt
      961 ctggtggtac gattgatatg aacggtgctt tgaatgaaga aggaactaaa gcaaaaaatc
     1021 taccacttat aaattcttca ggtgcatttg ctattgggaa ttcaaataac gtaactataa
     1081 aaaatgtaac attcaaggat agttatcaag ggcatgctat tcaaattgca ggttcgaaaa
     1141 atgtattagt tgataattct cgttttcttg ggcaagcctt acccaaaacg atgaaggatg
     1201 ggcaaatcat aagtaaggag agcattcaga ttgaaccatt aactagaaaa ggttttcctt
     1261 atgccttgaa tgatgatggg aaaaaatctg aaaatgtgac tattcaaaat tcctattttg
     1321 gcaaaagtga taaatctggg gaattagtaa cagcaattgg cacacactat caaacattgt
     1381 cgacacagaa cccctctaat attaaaattt taaataatca ttttgataac atgatgtatg
     1441 caggtgtacg ttttacagga ttcactgatg tattaatcaa aggaaatcgc tttgataaga
     1501 aagttaaagg agagagtgta cattatcgag aaagcggagc agctttagta aatgcttata
     1561 gctataaaaa cactaaagac ctattagatt taaataaaca ggtggttatc gccgaaaata
     1621 tatttaatat tgccgatcct aaaacaaaag cgatacgagt tgcaaaagat agtgcagaat
     1681 atttaggaaa agtatcagat attactgtaa caaaaaatgt aattaataat aattctaagg
     1741 aaacagaaca accaaatatt gaattattac gagttagtga taatttagta gtctcagaga
     1801 atagtatatt cggtggtaaa gaaggaattg ttattgagga ttcaaagggt aaaataaccg
     1861 ttttaaataa ccaattttat aatttatccg gtaagtatat atcattcatc aaatctaatg
     1921 caaatgggaa agaatctgtt atacgtgata gcgatggtaa tttcaatatt gtaacggaga
     1981 atgggcttta caaaattgta acaaataatt taagtgataa aaacgaaaaa gaaaaaaaca
     2041 aagaggaaaa acaatctaat tcaaataatg taattgatag taaccagaag aacggagagt
     2101 ttaactcaag taaagataat agacaaatga atgacaagat cgacaataaa caagataata
     2161 agacagaaga agtaaactat aaaatagttg gagatggcag agaaactgaa aatcatatta
     2221 ataaatctaa agaaatagta gatgtaaaac aaaaattacc aaagactggt tcgaacaaga
     2281 ttatggaact attcttaaca gtgacaggaa ttggtttact tttgacacta aaagggttga
     2341 agtattatgg taaagataaa taaaatttgt tcgatacaag gaagttccgt agaaaatgag
     2401 gatattgtag gttcacaaaa tcagtatttt tggattattg gtggagctac agatttatat
     2461 aattctaaag aggagatagg ttattcagtc tcagaagttg tacatatttt atcagaaagt
     2521 ttgtcggtta attgtaaaga atcaaaaact cttaaacaaa tatttgaaac tgctttactc
     2581 gaggttaaag atgaaatcgg tttaaactca tatgaattaa cagagtatag taaaatgaaa
     2641 taataatagg acaaatcaat caggacagtc aaatcgattt ctaacaatgt tttagaagta
     2701 gaggtgtact attctagttt caatatacta tattatcgct taccaatatt tgctaaactt
     2761 tcagaaaaaa agttggagta tatgatgcta ggtgactgcg tcatgttagt aaacgaaatg
     2821 gaaataactg atcatagagt agataactta tttgaaaaag ggaaaaatga gataaaagat
     2881 tcaagtagta gatttagcaa taggacatat aaaagcttta gaaaaagtat ctgaaaaaac
     2941 agatgtttat atctataacc taggctcggg agaaggcact agtgtattac aacttgtaaa
     3001 tacatttgaa agtgttaata agatccctat tccttataaa atagttccaa gacgttcagg
     3061 agacgttgca acttattatg caaatgcaga caaagcatat aaggaattaa attggaggac
     3121 aacaaaatcg attgaagaca tgtgtagaga tacatggaat tggcaatcaa aaaatcccaa
     3181 tggctataat tgatgatact gtctatatga gtgctgtatt ttggaaaaat tacttaacct
     3241 tttaatgaaa gataaattaa aaaaaatgtt tgacagcgct tgacaaagga aacggtttca
     3301 ttgtataatg taaacacaat gacaccgatg tcaaatataa aaaagaaggt ctttatatga
     3361 aaaacttaac aaaaattaag ttcaaagaaa atggggaatt taatcatttc cctgggaata
     3421 cagttgtagc aaatctttat actaaacaag atttgatgga agttgttgat attattcaat
     3481 cacgttatag agaattacca tttatcgata agtttacttt aactccaagg aattctattc
     3541 acatgactgt aattgaattg ttgtgccatg aaaatcgtga aacggagttt tggagcagta
     3601 atcttcctct agatacacct ttacaggaaa tacatgatta ctttgctaaa caacttgaaa
     3661 tttttccatt gttggatgaa gaaattcata tgcgtataac tgaaatggga aaacaaaaca
     3721 tactagttga acctgcagat gaagcttctg caaagagatt agaagaaatt cgtacttatg
     3781 tctcagaaaa agcaggtgtt tgtttcccta atcatgatag atatcaattc catatttcaa
     3841 ttgggtatct tcggattcct ctaaccgaag aggaggaaga agagtttact aaagtcagag
     3901 cagaattaac tgaaatttta ttagagaaga ttccaactat tactgtaaac cgtattgact
     3961 atactgtatt tgaagatatg aggcaatttg ttccatatca cgaaaaattt aaataaaata
     4021 ataaaaaagg agaacttatc atgaaaaaaa tgaaagtttg gtctactgta cttgcaacgg
     4081 gagttgctct tactacactt gctgcttgct ctggaggttc aaattctacg actgcttctt
     4141 catctgaaga aaaagctgat aaaagtcaag aattagttat ctattcgaac tcagtctcaa
     4201 atggtcgtgg tgattggtta actgctaaag caaaagaagc tggttttaat ataaaaatgg
     4261 ttgatatcgc tggcgctcaa ttagcagacc gtgttattgc tgagaagaat aatgcagttg
     4321 cagatatggt atttggaatt ggtgctgttg actcaaataa aattagagat caaaaattac
     4381 tagtacagta caagcctaaa tggttagata aaattgatca atctttatca gataaagata
     4441 attattataa tcctgtgatt gttcaaccat tagttttaat tggggcgcct gatgtaaaag
     4501 aaatgcctaa agattggact gaattaggta gtaagtataa aggtaaatat tcaatttatg
     4561 gtcttcaagg tggtacagga cgggcaattc tagcaagtat cttagttcgt taccttgatg
     4621 aaaaaggaga tttaggcgta tcagaaaaag gatgggaagt tgcaaaagaa tactttgcaa
     4681 atgcctatac tcttcaaaaa ggtgaaagct cagttgttaa agcgttagat aaagaaagcc
     4741 caattcaata cggtatgatc tggggttctg gagcgttaat tggacaaaaa gaacaaaatg
     4801 ttgaattcaa agttatgact cctgagattg gtgtaccatt tgtaactgaa caaactatgg
     4861 ttttaagcac tagtaaaaaa caagcgttag ctaaagaatt tattgattgg tttggtcaat
     4921 cagaaattca agtagaatat agtaaaaaat ttggatctat tcctgccaat aaagatgccc
     4981 taaaagagtt acctgaagat acgaaaaaat ttgttgatca agtgaaacca caaaatattg
     5041 actgggaagc tgttggaaag catttggatg aatgggtaga aaaagctgaa ttagaatacg
     5101 taaaataaga aataagagtt attttttatg tccaagtctc tctattattt ctaaatttaa
     5161 ggaagaaata aagagaggct tttggtttta gtaatagagg gagttagtga gatgattaaa
     5221 tttgataata ttcaaattaa atatggtgat tttgttgcaa ttgataatct gaatttagat
     5281 atacatgaag gggaattctt tacatttctt gggccttcag gatgtggtaa atcaactact
     5341 ttgagagcat tggtaggttt tttagatcca tcatcaggaa gtattgaagt taatggaaca
     5401 gatgtcactc atttggaacc tgaaaagcgt ggaattggta ttgtatttca atcttatgcg
     5461 ctatttccaa ctatgactgt ttttgataat attgcatttg gtttaaaagt taagaaggta
     5521 gctccagatg ttattaaagc taaagtatca gcagtggcag caaaaattaa gatctctgat
     5581 caacagttac agcgtaatgt atcagaatta tctgggggtc aacaacaaag ggtagcattg
     5641 gctcgtgctc tggttcttga acctaaaatt ctttgtctag atgaaccatt gtcaaacctt
     5701 gacgcaaaat tacgtgtaga tttgagaaaa gagttgaaaa gacttcaaaa agagttaggt
     5761 attactactt tatatgttac tcatgatcaa gaggaagcct tgactttatc tgatagaatt
     5821 gcagtcttta acaatggata catcgaacag gtcggtacac cagtagagat ttatcataat
     5881 tctcaaactg aatttgtatg tgattttatt ggagatatta atgttttgac cgatgaaaca
     5941 gtccacgaag tattactgaa aaatacaagc gttttcttag aggataaaaa aggttacatt
     6001 cgattagaga aagttcgatt caatcgtgaa actgaacaag attttattct aaaagggaca
     6061 attattgatg ttgagttttc tggagttaca attcactata caataaaagt ttctgaaagt
     6121 cagattctta atgtaacaag tattgatagt caggctgcta ttagatctgt cggagaaagt
     6181 gtggaattat ttatcacacc atcagacgtt ctgcaatttt aaggaggtgc agtatgcgtc
     6241 ataaattaaa tttaaaagat tggcttattc gtttagggtt aatctggttc ttagtaacat
     6301 ttattattta tccaaacttt gatctagtag tgaatgtatt tgtaaaagga ggagaatttt
     6361 cccttgatgc tgtacatcgt gttctaaaat ctcagagggc acttcagagt attatgaaca
     6421 gttttaagtt agcattttca ctcattatta cagttaatgt cgtaggtatt ctttgtgttc
     6481 tatttacaga gtactttgat attaaaggtg ctaaaatttt aaaattaggt tatatgacct
     6541 ctttaattta tggaggagtg gttttagcga ctggatataa atttgtctat ggtccttatg
     6601 gattgattac aaaattttta caaaatgtta tcccttcttt agactctaac tggtttattg
     6661 ggtatggtgc agtcttattc attatgacat tttcaggaac tgctaatcat acattgtttt
     6721 taacaaatac aattcgaagc gttgactatc acactattga ggctgctcga aatatgggag
     6781 caaaaccatt tactgttttc cgaaaagtag tgttaccaac cttaattcca actctatttg
     6841 cacttactat tatggttttt cttagtggtt tatctgcagt agcagcaccc atgattgttg
     6901 gtggtaaaga atttcaaact ataaatccaa tgattattac atttgcaggg atggggaatt
     6961 ctcgtgattt agctgcctta cttgcaatta ttttaggtat tgcaactaca attttgctta
     7021 ctatcatgaa taagatagaa aaaggtggaa attatatttc tatctctaag actaaagcgc
     7081 ctcttaaaaa acaaaaaatt gcgtctaagc cttggaatat cattgctcac attgtagcat
     7141 atggattgtt cacagttttc atgcttccac taatttttat agtattatac tcatttacag
     7201 atccagttgc aattcaaaca ggtaacttaa cattatcaaa ctttacttta gaaaattatc
     7261 gcttattctt tagtaatagt gcggcattct ctccattctt ggtcagcttt atttattcta
     7321 ttattgctgc gacaacagca acaattctcg cagttgtatt tgctcgtgtt gtcagaaaac
     7381 ataaatctcg ttttgatttc ttatttgaat atggtgctct acttccttgg ttactaccaa
     7441 gtacactttt agcagtaagt ttattattta cttttaatca gccacaattt cttgtcttga
     7501 atcagatttt ggtaggtagt ttggtaattc tacttattgc atatatagtt gtaaaaatcc
     7561 cattttctta tagaatggta cgtgctattt tatttagtgt tgatgatgag atggaagatg
     7621 cagcaagaag tatgggtgct tcaccttttt atactatgat gaaggttatc attccattta
     7681 ttttaccggt tgttctctct gttattgctt taaactttaa ctctttatta actgacttcg
     7741 acttatctgt attcctttac caccccctag ctcaaccatt aggtattacg attcgatctg
     7801 caggtgatga aacagcaaca tctaatgcac aagctctggt atttgtttat acaattgttc
     7861 tgatgattat ttctggaacg gtattatact tcacacaaag accggggcgt aaagtaagga
     7921 aataatcatg acagccacta gtcttgggtt atcaaatatt gaaatagttg tcaggattgt
     7981 tttatcagta gtcattggta gtataattgg tttagagaga gggagcaaat cccagcctgc
     8041 aggcatccga acttatagta ttgtttgtct agctgcatgt ttgattatga tgacgaatga
     8101 atacgtatct tataaatttg ggacaggaga tcctacacga ttaggagctc aagttatatc
     8161 aggtgtgggt tttctaggcg ctggaacgat tcttattaca gataaaaaga aaattacagg
     8221 tctgacaact gcagcaggca tttgggcttc ggcaggaatt ggattagcta ttggagtagg
     8281 tttttatgag ggagctcttt tagtagccat ttctgtttgg ggtgtgatat ccatgttcca
     8341 accactaaaa aaatatctac aaaatcgttc taaaatgatt gaattgtata tagtagttaa
     8401 atctacagaa gcctataatc gtgtattggt ttattgtgca gaaaatggta tcagaatgac
     8461 cgattcaaga acagcgtttg gagatgttaa ttctgacaga attgaatact ttgatgttcc
     8521 agataaaaaa atagcgtcat ttattactct ggaattatca ggtagatttg aacatcttcg
     8581 actgatggaa gaaattgcta atattgtagg tgttatttat gtcgaagaaa ttagctgaaa
     8641 tttagtaaac aaagggacag atgtttttgt ccttttgttt tttagatatt ttaatcatct
     8701 aatttttgag tagatttttg ctcagaggtt tttatgaaag attataaaat caattttgat
     8761 ttaggtaaaa tagaatattt tgataataat tgtttgatac aagtatacaa atttatttct
     8821 ttttatgata tttgtgaaat ggtgtttgcc tttcatttac ctcctgatga gttgataaca
     8881 aatgttatct ttaaagaaaa aattaactct atgttaaaat gttacattga caggctatta
     8941 tatgttttta taaatcctac acattttact gaaaaagtta atctacaatt ttatggtagt
     9001 tttttctcat atgaatttat ttgtcgtgaa gttggaaaca ttcttaaaaa taaaggtgtg
     9061 aaatgcaatt taaacttttt tgaaggggaa gaatatttgt aaaattaatt ttgtgaattt
     9121 gtggatctta aataatttac taaattctct tgttaaaaaa aattaaatat tgtatagtaa
     9181 tattagaatg tttgtaatag aggactgaaa tgaaaaatat aaatgggaaa aaggtaacta
     9241 tttatgatat tgcacgttta tcaggttttt ctccaaaaac agtttcacga gttattaatg
     9301 gtggagtaaa tgttaaagaa gaaacttatc aagcgattca aaaagttata gaggaattgt
     9361 cgtatattcc aaatgcgtat gcaaaaaatt taactaaaaa agaagctata aatatattga
     9421 tttctgtaaa aaaaatcgac tcttttcctt taatttggtt tcatacgctg ttagataaag
     9481 tgttacggac gtgtaaagaa tttggtgtta atgctatagt tgagtacttt ggagaggagg
     9541 atacaattag taattcaatt atatcaagca caggtagcct agtagatggt gttattgttt
     9601 tttatgaaag tgtagatgat atcagaattc agtatttaaa gaagaaccat atgccttttc
     9661 ttgttttcgg tgaatctcaa acgtctggag tagtctatgt atctaataat aattttcaag
     9721 ctacttatga tatgatgaaa gctgtaactg aagaaaaatt taaaaatatg tggttgctta
     9781 tgggaggaga atcccatgtt aataaggatc gtgaaagagg tgtgcgttct tttctgaatg
     9841 ataaaaatta ctttatggat ttaaaagtca tttatggctt atctacaatt gattcagttt
     9901 attcttatgc aatgcagcac ttgactaccc aaaattatcc ggacattatt tttgtttcag
     9961 gagatgaaaa ggttcagggc ttgattcgtg cgtgctatga gaaaggaatt cttattccag
    10021 atgatatttc tatcattgga tttgataata ttcctatttc tcagtattat actccagctt
    10081 tgtctacaat tgctcccaat tatgttaaat tggctaaaga aatgatagaa ggtgttttag
    10141 caattattaa aggggaaagt gtcacatctg ttgaagtttc tcctaaattt gttaggagac
    10201 agagtttcta gtattgttta attcttgaaa atcctagtgg aaaagatgga gtgttctaaa
    10261 ataagttctg tttagcctcc attcagtttc tattgagttg gatccaattt ctaacaagtg
    10321 ggacatgttc atgttcgatc taaggaagat atggtcacat tcgaatgata tcaaaaaaat
    10381 cgtaaaaaag tcttgacaaa gaaaaaacaa agtattaaaa taagttcaac aaatcagaaa
    10441 agtaactatt tttgatcttc agggagcctg tggtgattgt gaataggtgg ttggaagtag
    10501 tgaaagtggg ctgattttga aaatgaattt gaaacaatga aaattcggtg cgcacacctt
    10561 acagtgcagc ttgttgttaa acaaggcaga gatgtaaagg gatagtccct ttataattga
    10621 ggtggcaccg cgttaccaac gccctcacac ggaagtatat tctgtgtgtg ggcttttttc
    10681 tatccgtcgt ttggtttatc ttttattagg gccttaagtc gctttgatga acttgagttc
    10741 tatctacagc tccttgcctc ctactaaaag taaacaaaaa ggctggacta atacgaaaaa
    10801 agctagtaat actcaatgaa aatcaaagag caaactagga aactagccgc aggttgctca
    10861 aaacactgtt ttgaggttgt ggatagaact gacgaagtca gctcaaaaca ctgttttgag
    10921 gttgcagata gaactgacga agtcagtaac catacctacg gcaaggcgac gttgacgcgg
    10981 tttgaagaga ttttcgaaga gtataaaggt gtaccgagat gaacatctct tcatcaatgt
    11041 ctctggtcgt ggaggcaagg acctagctgc gattgcagat tactagaagc taaaaaataa
    11101 tagatggaaa aattacagtc ttttctctca cagagagctt gtggttgctg aaaacaagca
    11161 gagaaagctg taaagttggg ttcatctgaa acgagagaag aaaacatcat tctcaaggga
    11221 gtgcccttta tcgcacagcc tgttacagga gatatctgag acaggaatga gagataggag
    11281 aaatcctata attgaggtgg caccgcgaat ttcgtcctca cgcaagttat tttgcgtggg
    11341 gatttttcat acagtcgcca cgaactagaa ttagaactga aagggaaatg acaatggaac
    11401 gaatcattca tggagatgtc ttatcaccaa tcttggctta tatgcgctta aaggggcaac
    11461 ataaggttat tttagagagt attccgagag acaaggaaac agctcgtttt tctatcctag
    11521 cctataatcc agtttttgag attaagtttg aaaatggagt tctctatcaa aatggtcaag
    11581 cgattgatcg ggatcccttg gatttccttt atgaagtgac tcataagagc cagcaccatt
    11641 cagacctccc ttttggtggg ggagccattg gttttgttgg ttacgatatg atttcgcttt
    11701 atgaagaaat tggtcaaatc cctgaggata caattgggac gccagacatg catttctttg
    11761 tctatgagag ctatatggtc tttgaccaca agaaggagaa gatacatgtc atagaggatg
    11821 cactttatag tgagcgcagt caagaggcct tggaaaaatc cttgaaccaa gtgcttgagg
    11881 aattacgcat tcctgctcca aatgaatttg aagacttgga tttatcacca cttgacttca
    11941 aaccgcatat cgctccacat aagtttgagg gaatggtaga aacagctcgt gacttgattc
    12001 gtaacggaga tatgttccaa tgtgtactca gccagcgttt ctcagcagaa gttactggaa
    12061 atccatttga cttctacaga aatctccgcg tgactaatcc atctaattac ctttatttct
    12121 atgattttgg ggattatcaa atcatcggag ctagtccaga aagtttggtt tctgtcaaaa
    12181 atggcatcgt gacaaccaat ccgattgcag ggacgcgacc aagaggggct acggatgaag
    12241 aagacaaggc cttggcgact gacctcctct ctgatgagaa ggaaacagca gaacatcgaa
    12301 tgttggtaga cttggggcgt aacgatattg gccgcatctc tgaaacggcc agtgtccaag
    12361 ttactaagta tatggaggtg gagctcttcc gctacgtcat gcatttgacc agtgtggtca
    12421 agggacgttt gcttgcagaa ctcactgcca tggatgcctt gaaagcaacg cttcctgctg
    12481 gaaccgtttc tggagcacca aaaattcgag ctatgagacg catctatgaa ctggaaacgg
    12541 aaaaacggag cgtatatgca ggagcaatcg gctacttgtc tgcgacgggt gatatggatt
    12601 tggccattgc tatccgaacc atgattctca aaaatcaaag agcctatgtg caggctgggg
    12661 cagggattgt ctacgactct atcgcccaaa acgaatacca agaaaccatt aacaaagcaa
    12721 aatctatgac tagaattgga gaactaagac catgatttta ttgattgaca actatgattc
    12781 ttttacctat aacttggccc aatacattgg gaattttgca gaagttcagg ttttgagaaa
    12841 tgatgattcc aagctgtatg aagaagctga aaaagcagat ggtctggtct tttctcctgg
    12901 tcctggttgg ccagttgatg ctggaaagat ggaagacatg atttgtgatt ttgcaggcaa
    12961 gaagccgatt ctagggattt gtttgggtca ccaagccatt gcagaagtct ttggtggtaa
    13021 gctaggtttg gctccaaaag tcatgcatgg gaaacagagc aatatcaact ttgaagcgcc
    13081 atctgttttg tatcaaggta ttgaggatgg acgtgcggtc atgcgttatc acagtatttt
    13141 gattgaggaa atgccagaag actttgaagt gacagctcgt tcgactgatg accaagccat
    13201 catggggatt caacataaaa acctaccgat ttatggcttc cagtaccatc cagagagcat
    13261 tggaacgcca gacggcttgt cttctattcg gaattttatc gagaaggttg taaagtgagg
    13321 aaactaggat gaaagagatt attgaaaaac tagcaaaatt tgaaaattta tcaggtgtgg
    13381 aaatgacgga tgtcattgag cgtatcgtaa ctgggcgtgt aactgaagcg cagattgctt
    13441 ctctcctctt agctcttaag atgaaggggg aaacacctga agaacgtaca gccattgccc
    13501 aagtcatgag aggacatgcc cagcatattc caactgagat tcatgatgct atggacaact
    13561 gtggtacagg tggggacaag tctttcagtt ttaatatttc cacaactgca gcctttgtct
    13621 tggctggtgg cggtattcac atggccaagc atggtaaccg ctcgatttct tctaaatctg
    13681 gttccgcaga tgtcctcgaa gccttgggaa tcaatcttga cctcaaacca gctgaactag
    13741 gtaaggtctt tgataaaact ggaatcgtct ttctcttcgc taaaaatatg cacccagcta
    13801 tgaaatacat catgccagct cgtttggaat tgggaattcc aacgattatg aacttgactg
    13861 gtccactgat tcatccaatg gctttagaaa cacagcttct tggaattagt cgtccagaac
    13921 tcctagaaag tacagctcag gttttgaaaa atatgggtcg caaacgtgcc atcgtggttg
    13981 ctggaccaga agggttggat gaagctggct tgaacggaac aaccaagatt gcacttcttg
    14041 aaaatggcga aatcagcttg tcaagcttta ctccagagga tttgggaatg gaagactatg
    14101 ctatggaaga tattcgtggt gggaatgctc aggaaaatgc agaaattttg cttagcgttc
    14161 tgaaaaacga agcaagtcca ttcttggaaa cgacagtctt gaatgctggt cttggtttct
    14221 atgctaatgg taagattgat agcatcaagg aaggagttgc cttggcccgt caagtgattg
    14281 ctagaggcaa ggcccttgaa aaactcagac tgttacagga gtaccaaaaa tgagtcagga
    14341 atttttagca cgaatcttag agcagaaggc gcgtgaggtg gagcagatga agctggagca
    14401 aatccagcct ctgcgccaga cctatcgctt ggcagaattt ttgaagaatc atcaggactg
    14461 cttgcaggta atcgctgagg tcaagaaagc tagccctagt ttgggagata tcaatctcga
    14521 tgtggatatt gtgcaacagg cccagactta tgaagaaaac ggagcagtga tgatttcggt
    14581 gttgacagat gaggttttct ttaaagggca tttggattat ctacgggaaa tttccagtca
    14641 ggtagagatt ccgacgctca acaaagactt tatcatagat gaaaagcaaa tcatccgcgc
    14701 tcgcaatgta ggtgcgacag ttatcttgct tattgtggca gccttgtccg aagaacgcct
    14761 caaggaactg tatgactacg cgacagagct tggtctggaa gtcttagtgg agactcacaa
    14821 tctagctgaa ctagaggtag cccacagact tggtgctgag attatcgggg tcaacaaccg
    14881 caacttgact acctttgagg tcgacttgca gaccagtgta gatttagccc cttactttga
    14941 ggaaggtcgc tattacattt ctgaatctgc cattttcaca gggcaggatg cggaacgact
    15001 agccccatac tttaacggaa ttttggtagg gacagctctt atgcaggcag aaaatgtggc
    15061 ccagagaatc aaggagttgc agattgacaa aggttaaaat ttgtggacta tcgaccaaag
    15121 aagcggtgga aacagccgtt tcagcaggag ccgactatat cggttttgtc tttgcaccta
    15181 gtaaaagaca ggtgacttta gaagaggcag ctgagttggc aaagcttatt cctgcagatg
    15241 tgaaaaaggt tggagtattt gtttcaccaa gtcgggtaga actgctggaa gcgattgaca
    15301 aagttggctt ggacttggtt caagttcacg gtcaggtggc agctgattta tttgagaatt
    15361 tgccttgtgc cagtattcag gctgtgcagg tagatggaaa tgggcatgtc cccaattctc
    15421 aggcagatta tctactcttt gatgcccctg tggcaggaag tggtcagtcc tttgattggg
    15481 gtcaactgga tacgactgga ctagcacagc ccttctttat cgcaggtggc cttaatgaag
    15541 ataatgtagt aaaagcaatt caacatttta ctccctatgc agtagatgta tcgagcggag
    15601 tggagacaga tggacaaaaa gatcatgaaa agattagaag atttatagag agggtaaagc
    15661 atggcatatc aggaaccaaa taaagatgga ttttacggaa aattcggcgg acgttttgtc
    15721 ccagaaacat tgatgacagc agttttggag ttggagaagg cctaccgtga aagtcaggca
    15781 gacccaagtt tccaagagga attaaaccag cttttgcgcc agtacgtagg acgtgaaact
    15841 cctctttatt acgcaaaaaa cttgacccag catatcggcg gagccaagat ttatctcaaa
    15901 cgggaagacc ttaaccatac aggagcccac aagattaaca atgccttggg acaagtttgg
    15961 cttgccaaac gcatgggtaa aaagaaaatt atcgcagaaa ctggtgctgg tcagcacggt
    16021 gtggcaactg caactgctgc ggccctcttt aacatggaat gtaccatcta catgggtgag
    16081 gaagatgtca aacgccaagc cctcaatgtc ttccgtatgg agcttttggg agctaaggtc
    16141 gaggccgtga cagatggttc gcgcgtgctc aaggatgcgg tcaatgcagc ccttcgttca
    16201 tgggtggcta atatagatga tacccactat atccttggtt ctgccttggg gcctcatcca
    16261 tttccagaaa ttgttcgtga cttccaaagt gtcattggtc gagaggctaa acaacagtac
    16321 cgtgacttga caggacaaaa tctgccagat gccctagtag cctgtgttgg tggtgggtcg
    16381 aatgccatcg gtctcttcca tccctttgta gaagatgagt cagtagccat gtatggagct
    16441 gaagcggctg gatttggtgt ggatacggag caccacgcag ctaccttgac caagggtcgt
    16501 ccaggtgtcc ttcacggttc cctcatggat gtgctccaag atgcccatgg tcaaattctt
    16561 gaagccttct ctatctcagc aggtttggac tatcctggta tcggtccaga gcattctcac
    16621 taccacgata ttaaacgtgc cagctatgtc cctgtgacag acgaagaagc cttggaagga
    16681 ttccaactct tgtctcgtgt ggaagggatt atcccagctt tggaatctag ccatgctatc
    16741 gcctttgcgg tgaaattggc caaagaactt ggaccagaaa agtctatgat tgtatgccta
    16801 tcaggtcgtg gggacaagga tgtggttcaa gtaaaagacc gcttggaagc agatgcagca
    16861 aagaagggag aagctcatgc ctaagacact aacaaaaaaa ttaaatgcta ttaaagcagc
    16921 tggaaaagga atttttgttc cctatatcat ggctggtgac cacgaaaaag gtttggatgg
    16981 tcttgctgaa acaattcact ttttagaaga tttgggtgtc tctgcaattg aagtgggtat
    17041 tcccttttca gaccctgttg cagatggacc tgttatcgaa gaagctggct tgcgcagttt
    17101 agcccacggg acctctaccc aggctttagt tgaaaccttg aaaaccattg aaacagagat
    17161 tccactggtc atcatgacct acttcaaccc cctctttcag tacggtgtgg agaactttgt
    17221 caaagatctg gctgatacag ctgtgaaggg cttgattatc ccagacctgc ctcatgaaca
    17281 tgccaacttt gtagaaccat ttttggcaga tacagatatc gccttgattc ctttagtaag
    17341 cttgaccaca ggaattgagc gccagaaaga gttgattgaa ggggcggagg gattcgtcta
    17401 tgccgttgcc atcaatgggg taacagggaa atcaggcaat taccgtgcag atttggacaa
    17461 gcacttggcg caacttcatc aagtggccga catcccagtc ttgacaggtt ttggtgtatc
    17521 tagtcaagcc gatctggaac gcttcaatgc ggtgtcagat ggtgttatcg tcggttcgaa
    17581 aatagtaaaa gctctccacc aaggagagcc gattcaggac tttatcaaac aagcagtagc
    17641 ttaccaaaaa taattaaaca agtagcaagt aagaggaaag gcacgaaagg aagtctttcc
    17701 tttttctttt gcaggcgaaa ggctagtttt atagtgaatt gaatctggaa tattacacta
    17761 cggttcctaa aatattttaa gaaagtgatt tgaatttccc aataaatttg ttcagttttt
    17821 aattgatttt actatagaag atataatttt ctaaattact ctgacacaga actttaagaa
    17881 aaagctaatt attgtagtaa tttctttaag aaaatgatat aattcattaa aaagtatttg
    17941 gaggaggaag aattatgttg aatcaggatc tctttgattc gcttgaggca caaaaaattg
    18001 tagatacttt gatgaaaggg caaaaagatt atgtagatga acgtctagaa aaaagagaga
    18061 caatgatagt gtcgaatggt tatgcatgga cgtgacctaa tcatattgat actgcatttg
    18121 catcagcaga tttgtttgag tataaattac aattagcagg acagacttgg ggatatttag
    18181 aatttgaaac aaatacagaa aaatatggga aagtattgtt aattataaag ggtaagaagc
    18241 gacttacgaa ccaatttcct ttgatacaaa aaaataagag tggctactta tttgaatatg
    18301 ctcagatgaa tacactttat cttaatcaac attcttccta caaaaatgat gaagatagtc
    18361 attcctttcc aattcagatg gagttagttt ctgatgaaat gattcaagaa attgaacaag
    18421 ctactaaaaa ttcgaatatc gaaaaattta tgattttaac ttatgaggcg gactcagaaa
    18481 acaatattat atctgtagat gttgttatgc ctgatgcacg aactggtcag ttacacttga
    18541 ttcaagattt gtctgagtat attcaatcaa gttcgtacca tttcgaagaa gctaaatatc
    18601 aagatattcc taatttttca gaattatcgg aaacagaaga ttttgaaatt attccaagaa
    18661 tagaaaaaca agaaggtcaa aagtagtaag gaatatgtaa atgtttaatg gtcgggtatt
    18721 gaaagaatta cggctgttaa atggtttaag tagagcagaa ttagctcaga gaattaattt
    18781 aacggaacaa gccatttggc agtttgagtc caacgaaacg aaacctaaat tatcaaccaa
    18841 aatgcatttg gccaaccaat ttcatgttga tttaacttat tttgaacagg aagaagagag
    18901 cattcgattt gattcttctg taattgcctt tagaaatgca gacctagcaa cacggaaaac
    18961 aatagatatt caaactatgt atttacataa ggtagatagt ttgattgatt attttgaaag
    19021 ttttgtaatt atacctaata ttacaattca tgacctaagt aatgtagtga gtgaatctta
    19081 tcataaggga gaatccattg aggaattggc tctttatgcc agggaaaaat taggtatttc
    19141 aaaagataat catgatttgc tttataaatt agaacgttca ggcatctata tcgtggaacg
    19201 attaattaat ggccaagctg atgcttatag cgcatggtca aaattgggaa gaccttatat
    19261 tgtgttggga acgaataaat catctgtacg tcgaaatttt gacttagctc atgagctagg
    19321 acatattctt ttacacaaat ataaagatat gaatgaagat ggcaatcgtt tggagcaaga
    19381 agcaaattat tttgcatcat gttttttatt gccaaaagaa gagtttttag tcaaatttga
    19441 agagagggtt ggcaagcgtg tcagcaatcc tgatagttat attttattga agtcggattt
    19501 gaatgtttcg atacaggctt tagagtatcg agcttttaag ttaggattat tgactccaaa
    19561 gcaacattct tacttttatc gtcaaattgc gcaaaaaggt tacaaaatga ttgaaccttt
    19621 ggatgatcaa atttttgtta aaaaaccaag caaagtaaag agtattctgg acgtcgtttt
    19681 gagtaatcat ctagtcagtc tagcgactat aatgtctaaa caaagtattc gtttacagtt
    19741 tataagcgaa atattttcag tcgaaatgaa attttttgat cagtatcaag aagatagaag
    19801 aacagatcga tttgataaca tcatcccttt gtacaaaaga aataatttat aatttaactg
    19861 tgaaataatt tcatgaagga catatggcag aaattggatt ttatcagaca agcagtagct
    19921 taccaaaaat aatcacacaa gcagcaagta agaggaaagg cacgaaagga agtctttcct
    19981 ttttcttttg caggagaaag gccagaatac ccgtcgcaga agcgaactga atcaagatca
    20041 gtaactccgt tacgctaaag acgagagcac aagaagctaa aaagaggaaa tctcctgcgc
    20101 ccatgcggat atcgataaaa tgagccaaaa ttccaagagc aaggaaggag accatgacca
    20161 gattccagcc agaggaagct attaggatta ggtggaaagt catccagacc agtaagggat
    20221 attcctgatg gcgaaagtcg tagatgccta aggtcaaacc agcggtgatt aggacgactt
    20281 gccccaagga aagcaattcc caagagtaaa gcagaaagag gagtcctaag actaattcaa
    20341 agagggcata ccagacagga tagcgaactt tgcagtagcg acagcgaaag cgattgaaga
    20401 cctgtgagag aatcggaatc aaatctaagg gacgcaagcg agtctgacag gaatcgcagt
    20461 gactggctga actgataatg gattgctctg gaaaacggtc aatgaccaaa ccaagaaagg
    20521 aagcgagaat gctcccgacg agaaaaaaat aaaaatcaat catacttatc tattcgtaaa
    20581 aaatgggaga aatagtataa tggagaaaca tagataagat ggtgaggaaa agatgacaat
    20641 tcgttttgaa gaaaaggtga gtatagaaaa cgctcagctc gtatgccaat ggtccaactc
    20701 cttaggcaaa gtctttcaag aacaatggat gggaccaagg attccttttc tactgaccct
    20761 tcaagccttg gaaggagtct tttcaatctt tgatgaacaa gagtttgtgg gacttatcca
    20821 gaaaatcagg ctagaagaca gcaatcttca tatcgggaga ttttttatca acccccagaa
    20881 acaggagcaa ggcttaggta gccaggcttt aaggaaattt gttagtttgg cctttgaaaa
    20941 tgaagatata gatagtattt ctctaaatgt cttcgaggca aatcaaagag ctcagaatct
    21001 ttaccaaaaa gaaggatttg aaatcgttca aatggttgaa gcacctgtac gaaa
//
LOCUS       DAVBGY010000037        20816 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVBGY010000037 DAVBGY010000000
VERSION     DAVBGY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20816
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.037"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(4..924)
                     /gene="cysK"
                     /locus_tag="VQ620_001356"
     CDS             complement(4..924)
                     /gene="cysK"
                     /locus_tag="VQ620_001356"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET2763965.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(1209..1844)
                     /locus_tag="VQ620_001357"
     CDS             complement(1209..1844)
                     /locus_tag="VQ620_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HET2763966.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVSVNLV"
     gene            1900..3198
                     /locus_tag="VQ620_001358"
     CDS             1900..3198
                     /locus_tag="VQ620_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2763967.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVIEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            3195..3857
                     /locus_tag="VQ620_001359"
     CDS             3195..3857
                     /locus_tag="VQ620_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET2763968.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQACQFWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            3937..4485
                     /gene="raiA"
                     /locus_tag="VQ620_001360"
     CDS             3937..4485
                     /gene="raiA"
                     /locus_tag="VQ620_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET2763969.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            4621..6594
                     /locus_tag="VQ620_001361"
     CDS             4621..6594
                     /locus_tag="VQ620_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET2763970.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            6591..7043
                     /gene="rplI"
                     /locus_tag="VQ620_001362"
     CDS             6591..7043
                     /gene="rplI"
                     /locus_tag="VQ620_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET2763971.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQDPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            7087..8439
                     /gene="dnaB"
                     /locus_tag="VQ620_001363"
     CDS             7087..8439
                     /gene="dnaB"
                     /locus_tag="VQ620_001363"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET2763972.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            8441..8707
                     /locus_tag="VQ620_001364"
     CDS             8441..8707
                     /locus_tag="VQ620_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763973.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            8796..10142
                     /gene="cbpD"
                     /locus_tag="VQ620_001365"
     CDS             8796..10142
                     /gene="cbpD"
                     /locus_tag="VQ620_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HET2763974.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      10225..10327
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10398..10688
                     /locus_tag="VQ620_001366"
     CDS             10398..10688
                     /locus_tag="VQ620_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HET2763975.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            10651..11409
                     /locus_tag="VQ620_001367"
     CDS             10651..11409
                     /locus_tag="VQ620_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2763976.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            11447..12454
                     /locus_tag="VQ620_001368"
     CDS             11447..12454
                     /locus_tag="VQ620_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2763977.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            12454..13182
                     /locus_tag="VQ620_001369"
     CDS             12454..13182
                     /locus_tag="VQ620_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2763978.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            13301..13759
                     /locus_tag="VQ620_001370"
     CDS             13301..13759
                     /locus_tag="VQ620_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HET2763979.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            13761..16193
                     /locus_tag="VQ620_001371"
     CDS             13761..16193
                     /locus_tag="VQ620_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2763980.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFMELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALVLGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     ALRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKVKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            16835..17488
                     /gene="rr06"
                     /locus_tag="VQ620_001372"
     CDS             16835..17488
                     /gene="rr06"
                     /locus_tag="VQ620_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HET2763981.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            17485..18816
                     /gene="hk06"
                     /locus_tag="VQ620_001373"
     CDS             17485..18816
                     /gene="hk06"
                     /locus_tag="VQ620_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HET2763982.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILFLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKGEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            18899..19426
                     /locus_tag="VQ620_001374"
     CDS             18899..19426
                     /locus_tag="VQ620_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HET2763983.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            19505..>20816
                     /gene="pspC"
                     /locus_tag="VQ620_001375"
     CDS             19505..>20816
                     /gene="pspC"
                     /locus_tag="VQ620_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HET2763984.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEGVRSGD
                     TPKVTSSGDEVESHLKKILSEIQTQLDRKRHAKTVALINKLQDIKRTYSYKLNVLEEK
                     PKAELTSKTKEELTAAFEQFKKDTLPTEPGKKVAEAEKKVAEAKKKAEDQKEEDRRNY
                     PTNTYKTLELEIAEFDVKVKEAELELLKEKAKKPLNEGTIKQAKEKVESKKAEATRLE
                     NIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPAPAPKPENPAEQPKAE
                     KPADQQAEEDYARRSEEEYNRLTQQQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGS
                     MATGWLQNNGSWYYLNSNGAMATGWLQNNGSWYYLNANGSMATGWLQNNGSWYYLNAN
                     GSMATGWLQYNGSWYYLNANGSMATGWLQYNGSWYYLNANGDMAT"
BASE COUNT         6527 a         3441 c         4725 g         6123 t
ORIGIN      
        1 cggttacaat tcataaagtg ctgtagagag ataacgttca ccgttatctg gtgctagggc
       61 aaggactttt ttacctgtac ctaatttttt ggcaacctcg atggctccgt agatagctgc
      121 agctgaggaa atccctacaa ggaagccttc ttttccacca atttcacgtc cgagtgcaag
      181 agcgtcatct gatgttacac gaacgatacc atcataggct ttagtatcaa gtgtatcagg
      241 aataaatcca gctgagatac cttgaatttt gtgaggacca ggtttttcac cagatagaat
      301 agcagattca tctgcttcta ctgcaaaaac ttgaatgtta gaattttctg atttgagtgc
      361 atgagaaaca ccagaaatcg ttccaccagt acctactcca gcaacaaagg catctaatcc
      421 atctttaccg aaagcagcta gtatctcagc tcctgttgtt ctttcgtgta cttctggatt
      481 agctggattg tcaaattgaa gaggaaggaa accatcacgt tcagcagcga tttcttgagc
      541 cttagcaata gcacctttca ttccctcgct accaggagtt aggacgagtt cagcaccata
      601 agcttggata attttacgtc gttctacact catagtttca ggcataacga tgacgacttt
      661 atacccttta gcagcaccta cccatgaaag tccaataccg gtgtttccac ttgttgcttc
      721 aacaatagta gaaccaagtt tcagaatacc atcttgttca gctttttcaa tcatgctaag
      781 ggcaatacgg tcttttacag atgaaccagg attaaatgct tcaagcttta tatagacgtc
      841 tgcagcacct tctggcacga tgttgttaag tttaacaatc ggtgtttgac cgattaattc
      901 agtaatgttg ttataaatag acatatgaat acctctttgt ataagatatc tttagtataa
      961 cgctatccat accatttgta aaatatagaa atcctatcga agcgatagga tttctatata
     1021 tcatagatca aagtcattaa ttttcaagtg attgcgttca gttaattcta gtaaataggt
     1081 gtgaaggaaa acgatatcag gtttcgtttg aattgcttca ttttctagta tattttggta
     1141 cgcatctttt tattttgctc gctaaagtag ttgtgagaat gtcaacgaaa cgctgtatta
     1201 ttcattgttt acactaagtt tacagaaact tcaacctctc gtaaaccttg gtcagttaaa
     1261 gtgacttttc cattaaaaaa ctccacaagt gcagctttaa tagtttcttt ttcttcttta
     1321 tcaacataaa tcatcgtatc gacttgatct gtaaagtttg tatccagctc catgagatca
     1381 tgttctttaa ggaagttact gtactcttgg tactgagcat aagacatttg aatagcaatg
     1441 ccagcctgtt cttttatttc aataatacca atttctttga cagctaaggc gacactgccg
     1501 gcgtaagcac gaattagtcc tccagcgcct agtttaatac caccaaagta gcgtgtcacg
     1561 accacacaga cattggtgag attgtgattt tctagtaccc caagcatggg aacaccagca
     1621 gtaccactag gctcaccatc atcacttgta cgtttaattt cactacgttc tccaataata
     1681 aaggcagagc agttatgtgt cgctttgtag tgttcttttt tgatggcagt aatgaagtca
     1741 cgagcctctt cttcgctata aacacgcttg gcatggcaga taaagcgaga ttttttgatt
     1801 tcttcttgga cttggccgtc ctctttaatt gtcctaaatt ccatgattta attgtactaa
     1861 aaaataatct aaagtgctag tttttacgaa taaagaagta tgaaagtaaa tttagattat
     1921 ctcggtcgtt tatttactga gaatgaatta acagaagaag aacgtcagtt ggcggagaaa
     1981 cttccagcaa tgagaaagga gaaggggaaa cttttctgtc aacgttgtaa tagtactatt
     2041 ctagaagaat ggtatttgcc catcggtgct tactattgtc gagagtgctt gctgatgaag
     2101 cgagtcagaa gtgatcaaac tttatactat tttccgcagg aggattttcc gaagcaagat
     2161 gttctcaaat ggcgcggcca attaactcct tttcaagaga aggtgtcaga gggactgctt
     2221 caagcagtag acaagcaaaa gccaacctta gttcatgcgg taacaggagc tggaaagaca
     2281 gaaatgattt atcaagtagt ggctaaagtg atcaatgcgg gtggtgcagt gtgtttggct
     2341 agtcctcgca tagatgtttg tttggagctg tacaagcgcc tgcaacagga tttttcttgc
     2401 ggtatagctt tgctacatgg agaatcggaa ccttattttc gaacaccact agttgttgca
     2461 acaacccatc agttattgaa gttttatcaa gcttttgatt tgctgatagt ggatgaagta
     2521 gatgcttttc cttatgttga taatcccacg ctttaccacg ctgtcaagaa tagtgtaaag
     2581 gagaatggat tgagaatctt tttaacagcg acttcgacca atgagttaga taaaaaggtc
     2641 cgtttaggag aactaaaaag actgagttta ccgagacggt ttcatggaaa tccgttgatt
     2701 attccaaaac caatttggtt atcggatttt aatcgctact tagacaagaa tcgtttgtca
     2761 ccaaagttaa agtcctatat tgagaagcag agaaagacag cttatccgtt actcattttt
     2821 gcttcagaaa ttaagaaagg ggagcagtta gaagaaatct tacaggagca atttccaaat
     2881 gagaaaattg gctttgtatc ttctgtaaca gaggatcgat tagagcaagt acaagctttt
     2941 cgagatggag aactgacaat acttatcagt acgacaatct tggagcgtgg agttaccttc
     3001 ccttgtgtgg atgttttcgt aatagaggcc aatcatcgtt tgtttaccaa gtctagtttg
     3061 attcagattg gtggacgagt tggacgaagc atggatagac cgacaggaga tttgcttttc
     3121 ttccatgatg ggttaaatgc ttcaatcaag aaggcgatta aggaaattca gatgatgaat
     3181 aaggaggctg gtctatgaag tgcttgttat gtgggcagac tatgaagact gttttaactt
     3241 ttagtagtct cttacttctg aggaatgatg actcttgtct ttgttcagac tgtgattcta
     3301 cttttgaaag aattggggaa gagaactgtc caaattgtat gaaaacagag ttgtcaacaa
     3361 agtgtcaagc ttgtcaattt tggtgtaaag aaggagttga agtcagtcat agagcgattt
     3421 ttacttacaa tcaagctatg aaggattttt tcagtcggta taagtttgat ggagacttcc
     3481 tgttaagaaa agttttcgct tcatttttaa gtgaggagtt gaaaaagtac aaagagtatc
     3541 aatttgttgt aattccccta agtcctgata gatatgctaa cagaggattt aatcaggttg
     3601 agggcttggt agaggcagca ggctttgagt atctagattt attagagaaa agagaagaga
     3661 gagccagttc ttctaaaaat cgttcagagc gcttggggac agaacttcct ttctttatta
     3721 aaagtggagt cactattcct aaaaaaatcc tacttataga tgatatctat actacaggag
     3781 caactataaa tcgtgttaag aaactgttgg aagaagctgg tgctaaggat gtaaaaacat
     3841 tttcccttgt aagatgagga aaaaatttgc aaaaatagaa tgaaagcgtt ataataaaat
     3901 cataaaaaca aaaatgttta gaaagaaggt actcatatga ttaaatatag tatccgtggt
     3961 gaaaacctag aagtaacaga agcaattcgt gattatgtag tttctaaact cgaaaagatc
     4021 gaaaagtact tccaaccaga acaagagttg gatgcccgaa ttaacttaaa agtttatcgt
     4081 gaaaaaacgg ctaaagtgga agtaacgatt ccgcttggat ctattactct ccgcgcagaa
     4141 gatgtatctc aagatatgta tggttcaatt gaccttgtaa ctgataaaat tgaacgtcag
     4201 attcgtaaaa ataaaacaaa aatcgagcgt aaaaataaaa ataaggtagc aactggtcaa
     4261 ttatttacag atgctttggt ggaagattca aatattgtcc agtctaaagt tgttcgttca
     4321 aaacaaattg atttaaaacc aatggatttg gaagaagcaa ttctacaaat ggatttattg
     4381 gggcatgatt tctttatcta tgtggatgtt gaagatcaga caaccaatgt gatttatcgt
     4441 cgtgaggatg gcgaaattgg tttgttagag gttaaagaat cttaatttca atatgtgtaa
     4501 aggtaggttt actgaattgt aaactacctt tttcttataa ttgatagaat tactgattac
     4561 acttttaaaa agtcgctttt tgaggggtat tatggtataa tgggtgccaa gaggttttga
     4621 atgaaaaaat tttatgtaag tccaattttt cctattctag taggattgat tgcgtttgga
     4681 gtcttatcca ctttcattat ttttgttaat aataatctgt tgacggtttt aattttgttt
     4741 ctttttgtag gaggctatgt ttttttattt aagaaactga gagtgcatta tacaaggagt
     4801 gatgtagaac agatacagta tgtaaaccac caagcggaag aaagtttgac agctctattg
     4861 gaacagatgc ctgtaggtgt tatgaaattg aatttatctt ctggagaggt tgagtggttt
     4921 aatccctatg ctgaattgat tttgaccaag gaagatggtg attttgattt agaagctgtt
     4981 caaacgatta tcaaggcttc agtaggaaat ccgtctactt atgccaagct tggtgagaag
     5041 cgttatgctg ttcatatgga tgcttcttcc ggtgttttgt attttgtaga tgtatccagg
     5101 gaacaagcca taacagatga attggtaaca agtcgaccag tgattgggat tgtctctgtg
     5161 gataattatg atgatttgga ggatgcaact tctgagtcag atattagtca aatcaatagt
     5221 tttgtagcta attttatatc agagttttca gaaaaataca tgatgttttc tcgtcgggta
     5281 agtatggatc gattttatct atttactgac tacacggtgc ttgagggctt gatgaatgat
     5341 aaattttctg ttattgatgc tttcagagaa gagtcgaaac agagacagtt gcccttgacc
     5401 ttaagtatgg gattttctta tggcgatgga aatcatgatg agatagggaa agttgctttg
     5461 ctcaatttga acttggctga agtacgtggt ggcgaccagg tggttgttaa ggaaaacgac
     5521 gaaacgaaaa atccagttta ttttggtggt gggtctgctg cttcaatcaa gcgtacacgg
     5581 actcgtacgc gcgctatgat gacagctatt tcagataaga ttcggagtgt agatcaggtt
     5641 tttgtagtcg gtcacaaaaa tttagacatg gatgctttgg gctctgctgt aggtatgcag
     5701 ttgttcgcca gcaatgtgat tgaaaatagc tatgctcttt atgatgaaga acaaatgtct
     5761 ccagatattg aacgagctgt ttcattcata gaaaaagaag gagttacgaa gttgttgtct
     5821 gttaaggatg caatggggat ggtgaccaat cgttctttgt tgattcttgt agaccattca
     5881 aagacagcct taacattatc aaaagaattt tatgatttat ttacccaaac cattgttatt
     5941 gaccaccata gaagggatca ggattttcca gataatgcgg tcattactta tatcgaaagt
     6001 ggtgcaagta gtgccagtga gttggtaacg gaattgattc agttccagaa ttctaagaaa
     6061 aatcgtttga gtcgtatgca agcaagtgtc ttgatggctg gtatgatgtt ggatactaaa
     6121 aatttcacct cgcgagtaac tagtcggaca tttgatgttg ctagctatct cagaacgcgc
     6181 ggaagtgata gtattgctat ccaggaaatc gctgcgacag attttgaaga atatcgtgag
     6241 gtcaatgaac ttattttaca ggggcgtaaa ttaggttcag atgtactaat agcagaggct
     6301 aaggacacga aatgctatga tacagttgtt attagtaagg cagcagatgc catgttagcc
     6361 atgtcaggta ttgaagcgag ttttgttctt gcgaagaata cacaaggatt tatctctatc
     6421 tcagctcgaa gtcgtagtaa actgaatgta caacggatta tggaagagtt aggcggtgga
     6481 ggccacttta atttggcagc agctcaaatt aaagatgtaa ccttgtcaga agcaggtgaa
     6541 aaactgacag aaattgtatt aaatgaaata aaggaaaagg agaaagaaga atgaaagtaa
     6601 tctttttagc agatgttaaa ggaaaaggta aaaaaggcga aattaaggaa gtaccaacag
     6661 ggtatgcgca aaactttctt atcaaaaaga atctagccaa agaagcgact gctcaagctg
     6721 taggtgaact tcgtggtaaa caaaaatcgg aagaaaaagc tcacgctgag atgattgcag
     6781 aaggaaaagc aattaaagca caacttgaag cagaagaaac tgttgtagaa tttgttgaaa
     6841 aagttggtcc agatggtcgt acctttggct ctattaccaa taagaagatt gcagaagaat
     6901 tgcaaaaaca atttggaatt aagattgata aacgtcatat tcaagtacaa gatccgattc
     6961 gagcggttgg tttgattgat gtgccagtga aaatctatca agatatcaca agtgtaatca
     7021 atcttcgtgt gaaagaagga taagtttaca ccttcttgac aagattgtaa aaggaaggga
     7081 agtctgatgg cagaagtaga agagttacga gtacaacctc aagatatctt agctgagcaa
     7141 tccgttttag gggctatctt tattgatgag agtaaacttg tttttgtgcg agaatacatt
     7201 gagtctcggg acttttttaa gtatgcccat cgtttgattt tccaagccat ggtcgattta
     7261 tccgatcgtg gtgatgccat agatgcaaca acggttcgta ctatccttga taatcaaggt
     7321 gatttacaga atattggtgg cttgtcttac ttggttgaga ttgttaattc tgtgccaact
     7381 tctgctaatg cggagtatta tgctaagatt gttgcagaaa aagcaatgct acgtcgttta
     7441 attgccaagt tgacagagtc tgtcaaccaa gcttacgaag cgtcacaacc agctgatgaa
     7501 attattgctc aggcagaaaa agggctgatt gatgtcagtg aaaatgcaaa tcgaagcggg
     7561 tttaagaaca ttcgagatgt gttgaatctc aactttggaa atctggaagc tcgctcgcaa
     7621 caaacgaccg atattacagg tattgcgaca ggttatcgtg atttggatca tatgacaaca
     7681 ggacttcatg aggaggagtt gattatctta gcagctcgtc cagcagttgg taagacagca
     7741 tttgccttga atatcgctca gaacattggg actaagttgg acaaaacggt tgctattttt
     7801 tcactcgaaa tgggtgcgga aagcttggta gatcgtatgt tagctgcaga aggcttagtg
     7861 gagtcacatt ctatccgtac agggcaattg acagatgagg agtggcaaaa atatactatt
     7921 gctcagggta atctagctaa cgccagtatc tatatcgatg atacgccagg tattcggatt
     7981 acagagattc gttctcgttc tcgtaaattg gctcaagaaa ctggaaatct tggtttgatt
     8041 ttgatagact atttgcaact tatcacggga actggtcgag aaaatcgtca acaagaagtt
     8101 tctgaaattt ctcgtcagtt gaaaatacta gccaaggaat tgaaggttcc agtaatcgct
     8161 ctgagtcagc tttctcgtgg tgtagaacaa cgtcaggaca agagaccggt cttgtctgat
     8221 attcgtgaat ctgggtctat tgagcaggac gctgatatcg tagcttttct ctatcgcgat
     8281 gactactatg aacgtggtgg tgaagaagag gagggtatcc caaataataa ggtggaagtt
     8341 attatcgaga aaaaccgtag tggagctcgt ggaacagtgg aattgatttt ccaaaaagaa
     8401 tacaataaat tttcaagtat ctcaaagagg gaggcataag atgtcagatg catttacaga
     8461 tgtagccaag atgaaaaaaa tcaaagaaga aatcaaggca catgagggac aagtcgtaga
     8521 aatgactttg gagaatggtc gtaagcgcca aaaaaataga ttgggtaagc taattgaagt
     8581 ttatccatct ctatttattg tggagtttgg ggatgtggaa ggagataaac aagttaatgt
     8641 ttacgttgaa tcctttactt actcagatat tcttacagaa aagaatttga ttcattatct
     8701 tgactaaagt gagaaatttt ctcacttttt cttttttctc cgaataattt aggtgaaggc
     8761 aatcatcgct ttatattatt tttcaaggag gaagaatgaa aattttaccg tttatagcaa
     8821 gaggaacaag ttattacttg aagatgtcag ttaaaaagct tgttcctttt ttagtagtag
     8881 gattgatgct agcagctggt gatagtgtct atgcctattc cagaggaaat ggatcgattg
     8941 cgcgtgggga tgattatcct gcttattata aaaatgggag ccaggagatt gatcagtggc
     9001 gcatgtattc tcgtcagtgt acttcttttg tagcctttcg tttgagtaat gtcaatggtt
     9061 ttgaaattcc ggcagcttat ggaaatgcga atgaatgggg acatcgtgct cgtcgggaag
     9121 gttatcgtgt agataataca ccgacgattg gttccattac ttggtctact gcaggaactt
     9181 atggtcatgt tgcctgggtg tcaaatgtaa tgggagatca gattgagatt gaggaatata
     9241 actatggtta tacagaatcc tataataaac gagttataaa agcaaacacg atgacaggat
     9301 ttattcattt taaagatttg gatggtggca gtgttgggaa tagtcaatcc tcaacttcaa
     9361 caggcggaac tcattatttt aagaccaagt ctgctattaa aactgaaccc ctagttagtg
     9421 caactgtgat tgattactat tatcctggag agaaggttca ttatgatcaa attctcgaaa
     9481 aagacggcta caagtggttg agttatactg cctataatgg aagctatcgt tatgttcaat
     9541 tggaggctgt gaataaaaat cctctaggta attctgttct ttcttcaaca ggtggaactc
     9601 attattttaa gaccaagtct gctatcaaaa ctgaacccct agttagcgga actgtgattg
     9661 attactatta tcctggggag aaggttcatt atgatcagat acttgaaaaa gacggctaca
     9721 agtggttgag ttatactgcc tataatggaa gtcgtcgcta tatacagcta gagggagtga
     9781 cttcttcaca aaattatcag aatcaatcag gaaatatctc tagctatgga tccaataata
     9841 gttcaactgt cggttggaag aaaataaatg gtagttggta tcatttcaaa tcaaatggtt
     9901 ctaaatcaac aggatggctg aaagacggtt ctagctggta ttatttgaaa ttatctggtg
     9961 aaatgcagac aggatggtta aaggagaatg gctcgtggta ttatctgggt agttcagggg
    10021 caatgaaaac aggctggtac caggtctctg gtaagtggta ttattcttac tcttcaggcg
    10081 ccttagctgt taatacgacg gtggatggct acagagtaaa cagtgatgga gaacgagtat
    10141 agaaaattgg agtaggagaa atttcctgct cttttctgta agcagtttcc ttgacatttt
    10201 tcttattttg cgctatcata tatacatata atatatggga gtctgtgtac agtctgagag
    10261 gaagtgttaa acttcgaccg cacctgatct gggtaatgcc agcggaggga acgatactta
    10321 gtctaatttt gcaccttttc catgtatggt aaaggttttt ctttttttta aaaggaaaac
    10381 gagaagagga ggttcttatg aaagcaagca ttgccttgca agttttaccc ctagcacagg
    10441 ggattgatcg gatagctgtt attgatcagg tcattgctta tctgcaaact caagaagtga
    10501 cgatggtagt gacaccattt gaaacggtct tggaagggga gtttgatgag cttatgcgca
    10561 ttctaaaaga agcgctggaa gtggcagggc aggaggcaga caatgtcttt gccaatgtca
    10621 aaataaatgt aggagagatt ttaagtattg atgagaaact tgaaaagtat actgagacga
    10681 cacattagtc tattgggctt tctcggagta ttgtcaatct ggcagttagc aggttttctt
    10741 aaacttctcc ccaagtttat cctgccgaca cctcttgaaa ttctccagcc ctttgttcgt
    10801 gacagagaat ttctctggca ccatagctgg gcgaccttga gagtggcttt actggggctg
    10861 attttgggag ttttgattgc ctgtcttatg gctgtgctca tggatagttt gacttggctc
    10921 aatgacctga tttaccctat gatggtggtc attcagacca ttccgaccat tgccatagct
    10981 cctatcctgg tcttgtggct gggttatggg attttgccca agattgtctt gattatctta
    11041 acaacaacct ttcccatcat cgttagtatt ttggacggtt ttaggcattg tgacaaggat
    11101 atgctgactt tgtttagtct gatgcgagca aacccttggc aaatcctgtg gcattttaaa
    11161 atcccggtta gcctgcctta cttttatgca ggtctgaggg tcagtgtctc ctacgccttt
    11221 atcacaactg tggtatctga gtggttggga ggttttgaag gtcttggtgt ttatatgatt
    11281 cagtctaaaa aactgtttca gtatgatacc atgtttgcca ttattattct ggtgtcgatt
    11341 atcagtcttt tgggtatgaa gctggtcgat atcagtgaaa aatatgtgat taaatggaaa
    11401 cgtacttaaa atagagcgtt tttgaaaaag aaaagaggaa atcaaaatga agaaaacatg
    11461 gaaagtgttt ttaacgcttg taacagctct tgtagctgtt gtgcttgtgg cctgtggtca
    11521 aggaactgct tctaaagaca acaaagaggc agaacttaag aaggttgact ttatcctaga
    11581 ctggacacca aataccaacc acacagggct ttatgttgcc aaggaaaaag gttatttcaa
    11641 agaagctgga gtggatgttg atttgaaatt gccaccagaa gaaagttctt ctgacttggt
    11701 tatcaacgga aaggcaccat ttgcagtgta tttccaagac tacatggcta agaaattgga
    11761 aaaaggagca ggaatcactg ccgttgcagc tattgttgaa cacaatacat caggaatcat
    11821 ctctcgtaaa tctgataatg taagcagtcc aaaagacttg gttggtaaga aatatgggac
    11881 atggaatgac ccaactgaac ttgctatgtt gaaaaccttg gtagaatctc aaggtggaga
    11941 ctttgagaag gttgaaaaag taccaaataa cgactcaaac tcaatcacac cgattgccaa
    12001 tggcgtcttt gatactgctt ggatttacta cggttgggat ggtatccttg ctaaatctca
    12061 aggtgtagat gctaacttca tgtacttgaa agactatgtc aaggagtttg actactattc
    12121 accagttatc atcgcaaaca acgactatct gaaagataac aaagaagaag ctcgcaaagt
    12181 catccaagcc atcaaaaaag gctaccaata tgccatggaa catccagaag aagctgcaga
    12241 tattctcatc aagaatgcac ctgaactcaa ggaaaaacgt gactttgtca tcgaatctca
    12301 aaaatacttg tcaaaagaat acgcaagcga caaggaaaaa tggggtcaat ttgacgcagc
    12361 tcgctggaat gctttctata aatgggataa agaaaatggt atccttaaag aagacttgac
    12421 agacaaaggc ttcaccaacg aatttgtgaa ataatgacag aaattagact agagcatgtc
    12481 agttatgcct atggtcagga gaggatttta gaggatatca acctacaggt gacttcaggc
    12541 gaagtggttt ccattctagg cccaagtggt gttggaaaga ccaccctctt taatctaatc
    12601 gctgggattt tagaagttca gtcagggaga attgtccttg atggtgaaga aaatcccaag
    12661 gggcgcgtga gttatatgtt gcaaaaggat ctgctcttgg agcacaagac ggtgcttgga
    12721 aatatcattc tgcccctctt gattcaaaag gtggataagg cagaagctat ttcccgagcg
    12781 gataaaattc ttgcgacctt ccagctgaca gctgtaagag acaagtatcc tcatgaactt
    12841 agcggtggga tgcgccagcg tgtagcctta ctccggacct acctttttgg gcacaagctc
    12901 tttctcttag atgaggcctt tagcgccttg gatgagatga caaagatgga actccacgct
    12961 tggtatcttg agattcacaa acagttgcag ctaacaaccc tgatcatcac gcatagtatt
    13021 gaggaggccc tcaatctcag cgaccgtatc tatatcttga aaaatcgccc tgggcagatt
    13081 gtttcagaaa ttaaactaga ttggtctgaa gatgaggaca aggaagtcca aaagattgcc
    13141 tacaaacgtc aaatcttggc agaattaggc ttagataagt agaaaaatag ggagttggtg
    13201 aagattatcc tttaccagcg ccctttttct tttaaaaatg agaaaatttc ggtataatag
    13261 tcaaacaagg tcaaggttta aagagagagg tgggtttgtt atgagattta aaaatacatc
    13321 ggatcatatt gaggcctaca tcaaggcgat tttagatcaa tctggtatcg tggagttgca
    13381 acggagtcag ttggcagata cctttcaggt tgttcctagt cagattaact acgtgatcaa
    13441 gacacgcttt acggaaagta gaggctactt ggttgaaagt aagcgtggtg gcggaggcta
    13501 cattcgtata ggacggattg agttttctag tcatcatgaa atgctccggg agctgcttta
    13561 ctcgattggt gagcgagtca gtcaagaaat ttatgaggat attctccagc ttttggttga
    13621 gcaggaattg atgaccaagc aggagatgaa tttgctagaa tcagtagctt tggatcgcgt
    13681 tttaggagaa gaagctccag ttgttcgagc aaacatgcta cgtcaggtca tacaagaggt
    13741 agatagaaaa gggaagtaag atgaactatt caaaagcatt gaatgaatgt atcgaaagtg
    13801 cctacatggt tgctggacat tttggagctc gttatctaga gtcgtggcac ttgttgattg
    13861 ccatgtctaa tcacagttat agtgtagcag gggcaacttt aaatgattat ccgtatgaga
    13921 tggaccgttt agaagaggtg gctttggaac tgactgaaac ggactatagc caggatgaaa
    13981 cctttatgga attgccgttc tcccgtcgtt tgcaggttct ttttgatgaa gcagagtatg
    14041 tagcgtcagt ggtccatgct aaggtactag ggacagagca cgtcctctat gcgattttgc
    14101 atgatggcaa tgccttggcg actcgtatct tggagagggc tggtttttct tatgaagaca
    14161 agaaagatca ggtcaagatt gctgctcttc gtcgaaattt agaagaacgg gcaggctgga
    14221 ctcgtgaaga tctcaaggct ttacgccaac gccatcgtac tgtggctgac aaacaaaatt
    14281 ctatggccaa tatgatgggc atgccgcaga ctcctagtgg tggtctcgag gactatacgc
    14341 atgatttgac agagcaagcg cgttctggca agttagaacc agtcatcggt cgggacaagg
    14401 aaatctcacg tatgattcaa atcttgagcc gtaagactaa gaacaaccct gtcttggttg
    14461 gggatgctgg tgtcgggaaa acagctctgg tgcttggtct tgctcagcgt attgccagtg
    14521 gtgatgtgcc tgcggaaatg gctaagatgc gcgtgttaga acttgatttg atgaatgtcg
    14581 ttgcagggac acgcttccgt ggtgactttg aagaacgcat gaataatatc atcaaggata
    14641 ttgaagaaga tggccaagtc atcctcttta tcgatgaact ccacaccatc atgggttctg
    14701 gtagcgggat tgattcgact ctggatgcgg ccaatatctt gaaaccagcc ttggcgcgtg
    14761 gagctttgag aacggttggt gccaccactc aggaagaata tcaaaaacat atcgaaaaag
    14821 atgcggctct ttctcgtcgt ttcgctaaag tgacgattga agaaccaagt gtggcagata
    14881 gtatgactat tttacaaggt ttgaaggcga cttatgagaa acatcaccgt gtacaaatca
    14941 cagatgaagc ggttgaaaca gcggttaaga tggctcatcg ttatttaacc agtcgtcact
    15001 tgccagactc tgctatcgac ctcttggacg aagcagcagc aacagtgcaa aataaggtaa
    15061 agtatgtaaa agcagacgat tcagatttga gtccagctga taaggccctg atggatggca
    15121 agtggaaaca ggcagcccag ctaatcgcaa aagaagagga agtacctgtc tacaaagact
    15181 tggtgacaga gtctgatatt ttgaccacct tgagtcgctt gtcaggaatc ccagttcaaa
    15241 aactgactca aacggatgct aagaagtatc taaatcttga agcagaactc cataaacgtg
    15301 tcatcggtca agatcaagct gtttcaagca ttagtcgtgc gattcgccgt aatcagtcag
    15361 gaattcgcag tcacaagcgt ccgatcggtt cctttatgtt cctaggaccc acaggtgtcg
    15421 gtaagaccga gttggccaag gctttggcag aagttctctt tgatgacgaa tcagccctta
    15481 tccgctttga tatgagtgag tatatggaga aattcgcagc cagccgtcta aatggagctc
    15541 ctccaggcta tgtgggttac gaagaaggtg gagagttgac agagaaggtt cgcaataaac
    15601 cctattccgt tctcctcttt gatgaggtag agaaggccca cccagatatt tttaatgttc
    15661 tcttgcaggt tctggatgac ggtgttttga cagatagcaa gggacgcaag gtcgattttt
    15721 caaataccat tatcattatg acatcgaacc taggtgcgac tgcccttcgt gatgataaga
    15781 ctgttggttt tggggctaag gatattcgtt ttgaccagga aaatatggaa aaacgcatgt
    15841 ttgaagaact gaaaaaagct tatagaccgg aattcatcaa ccgtattgat gagaaggtgg
    15901 tcttccatag cctatctagt gaccatatgc aggaagtggt gaagattatg gtcaagcctt
    15961 tagtggcaag tttggctgaa aaaggcattg acttgaaatt acaagcttca gctctgaaat
    16021 tgttagcaaa tcaaggatat gacccagaga tgggagctcg cccacttcgc agaaccctgc
    16081 aaacagaagt ggaggacaag ttggcagaac ttcttcttaa gggagattta gtggcaggca
    16141 gcacacttaa gattggtgtc aaagcaggcc agttaaaatt tgatattgca taaaagaata
    16201 aaagtatcag catctgacca taagtcacag tggagtgaaa ctcaatgaaa atcaaagagc
    16261 aaactaggca gctagccgca ggttgctcaa aacactggtt tgaggttgca gatagagctg
    16321 acgtggtttg aagagatttt cgaagagtat gaaactaaaa cctatagctt ctaaacgatc
    16381 cgtggttttc accattcaac acaaaattca tatgtttatt accctccgtc gtatttgtct
    16441 tagagcgtgt gtagtagaaa aagagcagtc ttatctgaaa tttttattct ttcaaaagag
    16501 acctgtttct tttttgcatg tcaaatccgt tctagctggt atttgaaaaa tcaaactaat
    16561 attcaatgaa aatcaaagaa caaactaggc agctagccac aggttgctca aaacactgtt
    16621 ttgaggttgt agatagagct gacgtagttt gaagagattt tcgaagagta taagctgcaa
    16681 gatgaatgat tttcttgtat tgacgttgtt gttgacaaaa agtagcggat aaatgaaatc
    16741 cattccatta tcatagatga taggctggta ggaaattttc aaatagcata caggaaatag
    16801 atgtatggag ttctggtagt agaaagggag agagatgaac attttagttg cagatgacga
    16861 ggaaatgatt agagaaggaa ttgcagcatt tctgacagaa gagggttatc atgtcattat
    16921 ggctaaggat ggacaagagg tcttggaaaa atttcaagat ctccctatcc atctcatggt
    16981 attggattta atgatgccta ggaagagtgg ttttgaagtg ttaaaagaaa tcaatcaaaa
    17041 gcacgatatt cctgtcatcg tcttgagtgc tctgggagat gaaactactc agtcacaggt
    17101 atttgatctc tatgctgatg atcatgtgac aaaacctttt tctttggtac tgcttgtcaa
    17161 gcgtattaag gcgcttatca gacgttacta cgtcatagag gatatttggc gatatcagga
    17221 tgtaacagtg gattttacct cttacaaagc acattataaa aatgaagaaa ttgatctcaa
    17281 accaaaggaa ttactggtac taaagtgttt gattcagcat aaaaatcaag ttttaagtag
    17341 agagcagata ttggaagaaa tttcaaaaga tgtagctgat ttaccttgtg atagggtcgt
    17401 tgatgtctat attcgtactc ttcgcaaaaa attagcttta gattgtatcg tgactgtgaa
    17461 aaatgttggg tataagatta gcttatgata aaaaatccta aattattaac caagtctttt
    17521 ttaagaagtt ttgcaattct aggtggtgtt ggtctagtca ttcatatagc tatttatttg
    17581 acctttcctt tttattatat tcaactggag ggggaaaagt ttaatgagag cgcaagagtg
    17641 tttacggagt atttaaagac taagacatct gatgaaattc caagcttact ccagtcttat
    17701 tcaaagtcct tgaccatatc tgctcacctt aaaagagata ttgtagataa gcgactccct
    17761 cttgtgcatg acttggatat taaagatgga aagctatcaa attatatcgt gatgttagat
    17821 atgtctgtta gtacagcaga tggtaaacag gtaacagtgc aatttgttca cggggtggat
    17881 gtctacaaag aagcaaagaa tattttgttt ttgtatcttc catatacatt tttggttaca
    17941 attgcttttt cctttgtttt ttcttatttt tatactaaac gcttgctcaa tcctcttttt
    18001 tacatttcag aagtgactag taaaatgcaa gatttggatg acaatattcg ttttgatgaa
    18061 agtaggaaag gtgaagttgg agaagttgga aaacagatta atggtatgta tgagcacttg
    18121 ttgaaggtta ttcatgagtt ggaaagtcgt aatgagcaaa ttgtaaaatt gcaaaatcaa
    18181 aaggtttcct ttgtccgtgg agcatcacat gagttgaaaa cccctttagc cagtcttaga
    18241 attatcctag agaatatgca gcataatatt ggagattaca aagatcatcc aaaatatatt
    18301 gcaaagagta taaataagat tgaccagatg agccacttat tggaagaagt actggagtct
    18361 tctaaattcc aagagtggac agagtgtcgt gagaccttga ctgttaagcc agttttagta
    18421 gatattttat cacgttatca agaattagct cattcaatag gtgttacaat tgaaaatcaa
    18481 ttgacagatg ctaccagggt cgtcatgagt cttagggcat tggataaggt tttgacaaac
    18541 ctgattagta atgcaattaa atattcagat aaaaatgggc gcgtaatcat atccgagcaa
    18601 gatggctatc tctctatcaa aaatacatgt gcgcctctaa gtgaccaaga actagaacat
    18661 ttatttgata tattctatca ttctcaaatc gtgacagata aggatgaaag ttccggtttg
    18721 ggtctttaca ttgtgaataa tattttagaa agctatcaaa tggactatag ttttctccct
    18781 tatgaacacg gtatggaatt taagattagc ttgtagacag attagttttt tattaaagtt
    18841 catatagggt taacataagt gtgttattct ttgtgtagat aaaagaaagg atactaatat
    18901 ggtattagcg attattttag taacattctt tattcgattg atttttttaa agcgttcgat
    18961 agagaatgag aaacgaatcc ttagcaatgg cgggaaagaa tttggagttg agaatacaaa
    19021 acgattaact atggctcata ttgtttttta tctctcttgc ttggttgagg caatggtgca
    19081 caagacaatt tttgatggca tgggcatggt tggtttagtc ttgcttattt tttctatgct
    19141 gatgttaatg ttggtgattc acttgttggg agatatttgg acagtgaagc ttatgcttgt
    19201 caataatcac aaatatgtag atcatatctt gtttaggaca gtaaaacatc ctaattactt
    19261 tttaaatatt cttcctgagt tgattggctt gaccttgttg agtcatgctt atgtgacttt
    19321 tgttttagtt tttccagttt atgcagttat tttgtatcga cgaatagctg aagaggaaaa
    19381 gctattacat gaagttataa tcccaaatgg aagcataaag agataaatac aaaattcgat
    19441 ttatatacag ttcatattga agtgatatag taaggttaaa gaaaaaatat agaaggaaat
    19501 aaacatgttt gcatcaaaaa gcgaaagaaa agtacattat tcaattcgta aatttagtat
    19561 tggagtagct agtgtagtag ttgctagctt gttcttagga ggagtagttc atgcagaagg
    19621 ggttagaagt ggggataccc ccaaggttac atctagtggg gatgaagtcg agtcgcatct
    19681 aaaaaaaata ttgagtgaga tccaaacaca attagataga aaacgacatg ccaaaactgt
    19741 agcgctaatt aacaagttgc aagacattaa gagaacgtat tcgtataaat taaatgtttt
    19801 agaagagaag ccgaaagctg agttgacgtc aaaaacaaaa gaagagttaa ccgcagcttt
    19861 tgagcaattt aaaaaagata cattaccaac agaaccagga aaaaaggtag cagaagctga
    19921 gaagaaggtt gcagaagcta agaaaaaagc cgaggatcaa aaagaagaag atcgtcgtaa
    19981 ctacccaacc aatacttaca aaacgcttga acttgaaatt gctgagttcg atgtgaaagt
    20041 taaagaagcg gagcttgaac tattaaaaga gaaagctaag aaacctttaa acgagggcac
    20101 aattaagcaa gcaaaagaga aagttgagag taaaaaagct gaggctacaa ggttagaaaa
    20161 catcaagaca gatcgtaaaa aagcagaaga agaagctaaa cgaaaagcag cagaagaaga
    20221 taaagttaaa gaaaaaccag ctgaacaacc acaaccagcg ccggctcctc aaccagaaaa
    20281 accagctcca gctccaaaac cagagaatcc agctgaacaa ccaaaagcag aaaaaccagc
    20341 tgatcaacaa gctgaagaag actatgctcg tagatcagaa gaagaatata atcgcttgac
    20401 tcaacagcaa ccgccaaaaa ctgaaaaacc agcacaacca tctactccaa aaacaggctg
    20461 gaaacaagaa aacggtatgt ggtacttcta caatactgat ggttcaatgg cgacaggatg
    20521 gctccaaaac aatggctcat ggtactacct caacagcaat ggcgctatgg cgacaggatg
    20581 gctccaaaac aatggttcat ggtactatct aaacgctaat ggttcaatgg caacaggatg
    20641 gctccaaaac aatggctcat ggtactacct aaacgctaat ggttcaatgg cgacaggatg
    20701 gctccaatac aatggttcat ggtactacct aaacgctaat ggttcaatgg cgacaggatg
    20761 gctccaatac aatggttcat ggtactacct aaacgctaat ggtgatatgg cgacag
//
LOCUS       DAVBGY010000038        20553 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVBGY010000038 DAVBGY010000000
VERSION     DAVBGY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20553
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.038"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            127..2109
                     /locus_tag="VQ620_001376"
     CDS             127..2109
                     /locus_tag="VQ620_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2763985.1"
                     /translation="MKSSKLLALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQGSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            2410..7713
                     /locus_tag="VQ620_001377"
     CDS             2410..7713
                     /locus_tag="VQ620_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HET2763986.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATANENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLFSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKTPGNSTWYKGNRVVAPEPGSVNRLSITLKSDGQLNASNNDVNLFDTVTLPGAVN
                     DNLKNEKKILLKAGTYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVNDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGQGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLVSDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(7880..10039)
                     /locus_tag="VQ620_001378"
     CDS             complement(7880..10039)
                     /locus_tag="VQ620_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HET2763987.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGIYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(10036..10632)
                     /gene="recU"
                     /locus_tag="VQ620_001379"
     CDS             complement(10036..10632)
                     /gene="recU"
                     /locus_tag="VQ620_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HET2763988.1"
                     /translation="MINYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            10698..11225
                     /locus_tag="VQ620_001380"
     CDS             10698..11225
                     /locus_tag="VQ620_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HET2763989.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            11295..11624
                     /gene="gpsB"
                     /locus_tag="VQ620_001381"
     CDS             11295..11624
                     /gene="gpsB"
                     /locus_tag="VQ620_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HET2763990.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            11645..12025
                     /gene="rnpB"
                     /locus_tag="VQ620_001382"
     ncRNA           11645..12025
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VQ620_001382"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            12110..13267
                     /locus_tag="VQ620_001383"
     CDS             12110..13267
                     /locus_tag="VQ620_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET2763991.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARLPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERKLELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            13280..14650
                     /gene="mapZ"
                     /locus_tag="VQ620_001384"
     CDS             13280..14650
                     /gene="mapZ"
                     /locus_tag="VQ620_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HET2763992.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTNSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQASSNTTS
                     EPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAWDFADGVLE
                     QILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGYFVGNGAGH
                     ADDLDY"
     gene            14726..16150
                     /gene="gndA"
                     /locus_tag="VQ620_001385"
     CDS             14726..16150
                     /gene="gndA"
                     /locus_tag="VQ620_001385"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET2763993.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            16162..16851
                     /locus_tag="VQ620_001386"
     CDS             16162..16851
                     /locus_tag="VQ620_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2763994.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            16949..17905
                     /gene="cbpC"
                     /locus_tag="VQ620_001387"
     CDS             16949..17905
                     /gene="cbpC"
                     /locus_tag="VQ620_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HET2763995.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDDLFDNRPVFEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLRSSGAMA
                     TGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMATGWYQDGSTWYYLNAGN
                     GDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(18023..19255)
                     /locus_tag="VQ620_001388"
     CDS             complement(18023..19255)
                     /locus_tag="VQ620_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2763996.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVHIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFVTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(19298..19885)
                     /locus_tag="VQ620_001389"
     CDS             complement(19298..19885)
                     /locus_tag="VQ620_001389"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2763997.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCEIAY
                     ALFAYSKVLEPSMFYNDLHTFHAELKKLLEKVTSNTENLGNLQLSWCEGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVILA
                     CSERDDHGLLMFQGDSGKADLFDFGIGSRGYIGVY"
     gene            complement(19785..20121)
                     /locus_tag="VQ620_001390"
                     /pseudo
     CDS             complement(19785..20121)
                     /locus_tag="VQ620_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
BASE COUNT         6407 a         3711 c         4597 g         5838 t
ORIGIN      
        1 ggttacgata ttcatatttt ttgcaaagat gttatttgaa aaataatttt caaaaattct
       61 gaaaattctg ttgacaactt tctgaaaaga gtctataatg gagagaaagt tttaaaggag
      121 aaaaggatga aaagttcaaa actacttgcc cttgcgggcg tgacattatt ggcggcgact
      181 actttagctg catgctctgg atcaggttca agcactaaag gtgagaagac attctcatac
      241 atttatgaga cagaccctga taacctcaac tatttgacaa ctgctaaggc tgcgacagca
      301 aatattacca gtaacgtggt tgatggtttg ctagaaaatg atcgctacgg gaactttgtg
      361 ccgtctatgg ctgaggattg gtctgtatcc aaggatggat tgacttacac ttatactatc
      421 cgtaaggatg caaaatggta tacttctgaa ggtgaagaat acgcggcagt caaagctcaa
      481 gactttgtaa caggactaaa atatgctgct gataaaaaat cagatgctct ttaccttgtt
      541 caagaatcaa tcaaagggtt ggatgcctat gtaaaagggg aaatcaaaga tttctcacaa
      601 gtaggaatta aggctttgga tgatcagaca gttcagtaca ctttgaacaa accagaaagc
      661 ttctggaatt ctaagacaac catgggtgtg cttgcgccag ttaatgaaga gtttttgaac
      721 tcaaaagggg atgattttgc caaagcgacg gatccaagta gtctcttgta taacggtcct
      781 tatttgttga aatccattgt gacaaaatcc tctgttgaat ttgcgaaaaa tccgaactac
      841 tgggataagg acaatgtgca tgttgacaaa gttaaattgt cattctggga tggtcaagat
      901 accagcaaac ctgcagaaaa ctttaaagat ggtagcctta cagcagctcg tctctatcca
      961 acaagtgcaa gtttcgcaga gcttgagaag agtatgaagg acaatattgt ctatactcaa
     1021 caaggctcta ttacgtatct agttggtaca aatattgacc gtcagtccta taaatataca
     1081 tctaagacca gcgacgaaca aaaggcatcg actaaaaagg ctctcttaaa caaggatttc
     1141 cgtcaggcta ttgcctttgg ttttgatcgt acagcctatg cctctcagtt gaatggacaa
     1201 actggagcaa gcaaaatctt acgtaatatc tttgttccac caacatttgt tcaagcagat
     1261 ggtaaaaact ttggcgatat ggtcaaagag aaattggtca cttatgggga tgaatggaag
     1321 gatgttaatc ttgcagattc tcaggatggt ctttacaatc cagaaaaagc caaggctgaa
     1381 tttgctaaag ctaaatcagc cttacaagca gaaggtgtga ctttcccaat tcatttggat
     1441 atgccagttg accagacagc aactacaaaa gttcagcgcg tccaatctat gaaacaatcc
     1501 ttggaagcaa ctttaggagc tgataatgtc attattgata tccaacaact acaaaaagac
     1561 gaagtaaaca atattacata ttttgctgaa aatgctgctg gcgaagactg ggatttatca
     1621 gataatgtcg gttggggtcc agactttgcc gatccatcaa cctaccttga tattatcaaa
     1681 ccttctgtag gagaaagtac taaaacatat ttagggtttg actcagggga agataatgta
     1741 gctgctaaaa aagtaggtct atatgactac gaaaaattgg tcactgaggc tggtgatgag
     1801 gctacagatg ttgctaaacg ctatgataaa tacgctgcag cccaagcttg gttgacagat
     1861 agtgctttga ttattccaac tacatctcgt acagggcgcc caatcttgtc taagatggta
     1921 ccatttacaa taccatttgc attgtcagga aataaaggta caagtgaacc agtcttgtat
     1981 aaatacttgg aacttcaaga caaggcagtc actgtagatg aataccaaaa agctcaggaa
     2041 aaatggatga aagaaaaaga agagtctaat aaaaaggctc aagaagatct cgcaaaacat
     2101 gtgaaataac tgttgcaaaa tataagaaag gatttagtat ttctcttgaa tgctgaatcc
     2161 ttttttacat ttgtaaagaa agattctaaa tgtactgacc cccaaaagtt ggagcctctt
     2221 tttgtcagaa tagagaaaat ttttgttaat tttacttgtt tcctattgct ttctcagcta
     2281 ttatttgtta tattaaaagt ataattattt tttatttatc agggttaagc attgcacttt
     2341 cagaggaagg agtatttttt aaaaaggaaa tgtaaacgct tgctaaaaaa tgaaaggatt
     2401 tagaagttta tgaataaagg attatttgaa aaacgttgta aatatagtat tcggaaattt
     2461 tcattaggtg ttgcttctgt tatgattgga gctgcattct ttgggacaag tccggttctt
     2521 gcagatagcg tgcagtctgg ttccacggcg aacttaccag ctgatttagc tactgctctt
     2581 gcaacagcaa acgagaatga tgggcgtgat tttgaagcgc ctaaggtggg agaagaccaa
     2641 ggttctccag aagttacaga tggacctaag acagaagaag aactattagc acttgaaaaa
     2701 gaaaaaccgg ctgaagaaaa accaaaagag gataaacctg cagctgctaa acctgaaaca
     2761 cctaagacgg taactcctga atggcaaacg gtagagaaaa aagaacaaca gggaacagtc
     2821 actatccgag aagaaaaagg tgtccgctac aaccaactat tctcaactgc tcaaaatgat
     2881 aacgcaggca aaccagccct gtttgaaaag aagggcttga ccgttgatgc caatggaaat
     2941 gcaactgttg atttaacctt caaagaagat tctgaaaagg gcaaatcacg ctttggtgtc
     3001 ttcctgaaat ttaaagatac caataataat gtttttgtcg gttatgacaa ggatggctgg
     3061 ttctgggaat acaaaactcc aggtaatagc acatggtata aaggcaaccg tgtagttgca
     3121 ccagaaccgg gttctgtaaa ccgtctttct atcactctca agtcagatgg acaactcaat
     3181 gccagcaata acgatgtcaa tctctttgac acagtgacac tgcctggggc ggtcaatgat
     3241 aatcttaaaa atgagaagaa gatccttctc aaggcaggaa cttatggcaa tgaccgtaca
     3301 gttgtcagcg ttaaaacaga caatcaagaa ggcgtaaaag cggatgatac tcctgcccag
     3361 aaagaaacag gtccggttgt tgacgatagc aaggtgactt atgatacgat ccagtctaag
     3421 gtcctcaaag cagtgattga ccaagccttc cctcgtgtca aggaatacag cttgaatgga
     3481 catactttgc caggacaggt gcaacagttc aaccaagtct ttatcaataa ccaccgaatc
     3541 acccctgaag tcacttataa gaaaatcaat gagacaacag cagagtactt gatgaagctt
     3601 cgcgatgatg ctcacttaat caatgcggaa atgacagtac gcttgcaagt tgtggacaat
     3661 caattgcact ttgatgtgac caagattgtc aaccacaatc aagtcactcc aggtcaaaag
     3721 attgatgacg aaagaaaact actttcttct attagtttcc tcggcaatgc tttagtctct
     3781 gtttctagtg atcaaactgg tgctaagttt gatggggcaa ccatgtcaaa caatacgcat
     3841 gtcagcggag atgatcatat cgatgtaacc aatccaatga aagacctagc caagggttac
     3901 atgtatggat ttgtttctac agataagctt gctgctggtg tttggagtaa ctctcaaaat
     3961 agctatggtg gtggttcgaa tgactggact cgtttgacag cttataaaga aacagtcgga
     4021 aatgccaact atgtaggaat ccacagctct gaatggcaat gggaaaaagc ttataagggc
     4081 attgttttcc cagaatacac gaaggaactt ccaagtgcta aggttgttat cactgaagat
     4141 gccaatgcag acaagaaagt cgattggcaa gatggtgcca ttgcttatcg tagcattatg
     4201 aacaatcctc aaggttggga aaaagttaag gatatcacag cttaccgtat cgcgatgaac
     4261 tttggttctc aagcacaaaa tccattcctt atgaccttgg atggtatcaa gaaaatcaat
     4321 ctccacacag atggtcttgg gcaaggtgtt ctccttaaag gatatggtag cgaaggccat
     4381 gactctggtc acttgaacta tgctgatatt ggtaagcgta tcggtggtgt cgaagacttc
     4441 aagaccctaa ttgagaaggc taagaaatat ggagctcatc taggtatcca cgttaacgct
     4501 tcagaaactt atcctgagtc taaatacttc aatgaaaaaa ttctccgtaa gaatccagat
     4561 ggaagctata gctatggttg gaactggcta gatcaaggta tcaacattga tgctgcctat
     4621 gacctagctc atggtcgttt ggcacgttgg gaagatttga agaaaaaact tggtgacggt
     4681 ctcgacttta tctatgtgga cgtttggggt aatggtcaat caggtgataa cggtgcctgg
     4741 gctacccacg ttcttgctaa agaaattaac aaacaaggct ggcgctttgc gatcgagtgg
     4801 ggccatggtg gtgagtacga ctctaccttc catcactggg cagctgactt gacctacggt
     4861 ggctacacca ataaaggtat caacagtgcc atcacccgct ttatccgtaa ccaccaaaaa
     4921 gatgcttggg ttggggacta cagaagttat ggtggtgcag ccaactatcc actgctaggt
     4981 ggctacagca tgaaagactt tgaaggctgg cagggaagaa gtgactacaa tggctatgta
     5041 accaacttat ttgcccatga cgtcatgact aagtacttcc aacacttcac tgtaagtaaa
     5101 tgggaaaatg gtacaccggt gactatgacc gataacggta gtacctataa atggactcca
     5161 gaaatgcgag tggaattggt agatgctgac aataataaag tagttgtaac tcgtaagtca
     5221 aatgatgtca atagtccaca atatcgcgaa cgtacagtaa ctctcaacgg acgtgtcatc
     5281 caagatggtt cagcttactt gactccttgg aactgggatg caaatggtaa gaaactttct
     5341 actgataagg aaaagatgta ctacttcaat acgcaggccg gtgcaacaac ttggaccctt
     5401 ccaagcgatt gggcaaagag caaggtttac ctttacaagc taactgacca aggtaagaca
     5461 gaagagcaag aactaactgt aaaagatggt aaaattaccc tagatcttct agcaaatcaa
     5521 ccatacgttc tctatcgttc aaaacaaacc aatcctgaaa tgtcatggag tgaaggcatg
     5581 cacatctatg accaaggatt taacagtggt accttgaaac attggaccat ttcaggcgat
     5641 gcttctaagg cagaaattgt caagtctcaa ggggcaaacg atatgcttcg tattcaagga
     5701 aacaaagaaa aagttagtct cactcagaaa ttaactggct tgaaaccaaa taccaagtat
     5761 gccgtttatg tcggtgtcga taaccgtagt aatgccaagg caagtatcac tgtgaatact
     5821 ggtgaaaaag aagtgactac ttataccaat aagtctctcg ccctcaacta tgtaaaagcc
     5881 tacgcccaca atacacgtcg taacaatgct acagttaacg atacaagtta cttccaaaac
     5941 atgtacgcct tctttacaac aggatcggac gtctcaaatg ttactctgac attgagtcgt
     6001 gaagctggtg atcaagcaac ttactttgat gaaattcgta cctttgaaaa caattcaagc
     6061 atgtatggag acaagcacga tacaggtcaa ggaaccttca agcaagactt tgaaaatgta
     6121 gctcaaggaa tcttcccatt tgtagtgggt ggtgtcgaag gtgtcgaaga caaccgcact
     6181 cacttgtctg aaaaacacga tccatataca caacgtggtt ggaacggtaa gaaagtcgat
     6241 gatgttatcg aaggaaattg gtcactcaag acaaatggac tagtgagccg tcgtaacttg
     6301 gtttaccaaa ccatcccaca aaacttccgt tttgaagcag gtaagaccta ccgtgtaacc
     6361 tttgaatacg aagcaggttc agacaatacc tatgcttttg tagtcggtaa gggagaattc
     6421 cagtcaggtc gtcgtggcac tcaagcaagc aacttggaaa tgcatgaatt gccaaatact
     6481 tggacagatt ctaagaaagc caagaaggca accttccttg tgacaggtgc agaaacaggg
     6541 gatacttggg taggtatcta ctcaactggc aatgcaagta atactcgtgg tgattctggt
     6601 ggaaatgcca acttccgtgg ttataacgac ttcatgatgg ataatcttca aatcgaagaa
     6661 attaccctaa caggtaagat gttgacagaa aatgctctga agaactactt gccaacggtt
     6721 gccatgacta actacaccaa agagtctatg gatgctttga aagaggcggt ctttaacctt
     6781 agtcaggccg atgatgatat cagtgtggaa gaagcgcgtg cagagattgc caagattgaa
     6841 gccttgaaga atgctttggt tcagaagaaa acggctttgg tagcagatga ctttgcaagt
     6901 cttacagctc ctgctcaggc tcaagaaggt cttgcaaatg cctttgatgg aaacttatct
     6961 agtttatggc atacatcttg gaatggtgga gatgtaggca agcctgcaac catggtcttg
     7021 aaagaaccaa ctgaaatcac tggacttcgt tacgttccac gtggatcagg ttcaaatggt
     7081 aacttgcgtg atgtgaaact tgttgtgaca gatgagtctg gcaaggagca tacctttact
     7141 gcaactgatt ggccagataa caataagcca aaagacattg attttggtaa gacaattaag
     7201 gctaagaaaa ttgtccttac aggtactaag acttacggag atggtggcga taaataccaa
     7261 tctgcagcgg aactcatctt tactcgtcca caggtagcag aaacacctct tgacttgtca
     7321 ggctatgaag cagctttggc taaagctcag aaattaacag acaaagacaa tcaagaggaa
     7381 gtagctagcg ttcaggcaag catgaaatat gcgacggata accatctctt gacggaaaga
     7441 atggtggaat actttgcaga ttatctcaac caattaaaag attctgctac gaaaccagat
     7501 gctccaactg tagagaaacc tgagtttaaa cttagctctt tagtttccga tcaaggtaag
     7561 acgccagatt ataagcaaga aatagctaga ccagaaacac ctgaacaaat cttgccagca
     7621 acaggtgaga gtcaatctga cacagccctc ttcctagcaa gtgttagtct agccctatct
     7681 gctctctttg tagtaaaaac gaagaaagac tagtatttag taaaacctct taacaagatt
     7741 acggaagcag tctctatctt ttccaatgag gtttatagta cagaaaaagc ctgagaagac
     7801 atcttctcag gcttttgtta agcacataaa tacaatagtg ctatgacaaa atcacccaga
     7861 aaaatctggg tgataaatgt tatggttgtg ctggttgagg attctgattt tgttgatcag
     7921 gggttgtatt tgattgttgc gtattattgt taggattggt agtcgtacta ttatttgtgc
     7981 ttggagtggt tgagctagac tgtgaagttg aactatctga tgatgagctt gaactttcag
     8041 ttgatggggg ttgttgtgga gcaggtgagc tccacgtaga acgagcacca tttttaaata
     8101 cgaattctcc atttctgtag agcccctctg gtatattcca atcctctgga ttgcttcctt
     8161 cagacaggta ggtcatcata gagcggtaaa ctttggcagc gaccgtaagg ccattgccta
     8221 caagtggtgt cagacggtta gaatagcctg tccatacagc cattgaatat ttacgcgtat
     8281 agccagcaaa tagttcatca ggtgctacaa attgagaggt cttgatgtgg ttttcaattt
     8341 cctcgtctgt atagttagag gttcctgttt taccagcctg agggagccaa gcaagatagg
     8401 catttcgtcc agttccataa gtcaagactg ttttcatcat gtcggtcatc atataggctg
     8461 tcgtttcctt catggcacga gttccgacat tagagaactc tttttcactc ccatcactaa
     8521 agacgacttt atggatatac attggtttat agtaagttcc accatttgca aaggcagcgt
     8581 aagcagcagc catcttttca ctacttgctc catatttttt gtctgattcg gttgtgttac
     8641 ttgaaatggc atttgagtag tgaatacttg ggtagtcgat tccgagacca tttaggaaag
     8701 tcttggcgcg gttgagtccg accttgttta gagtttccac ggctgggacg tttcgcgatt
     8761 gttgcagggc gtattgcaag gtgatgttgc caaagtagcc cctatcccag ttataaacag
     8821 gggtatttgt cccagggtag ttatagggct catcgtgaac gatagtagca gttgaatcgt
     8881 agataccgta ctccaaggca ggagcatagt ctgtgatcgg tttcatagtt gatccccagt
     8941 cgcggtttgt ttctactgct tggttaattc cgaaggaaac attacttgac tgatggcgtg
     9001 ctcctagctg ggcaatgact ttaccgttag aaacatcaac aatggtagaa gcgacttgca
     9061 attcatcgtc tggataggca acgtattcgt ctgtattgta aatatcccac agatgttttt
     9121 gagcttcttg gtctacattt gtgtagacat ccatcccagt tgtgagcagg ttatagcctg
     9181 tttcttcttc aacttgattg atgacttcct tgaggtaatt atccatgtaa gcagggtaat
     9241 tacttgctga tttgagactt tgtagtccat cagtaattgg tgtattgact gctttctcat
     9301 actgttcagc agagatgtag ccttgatttt tcatttcaga taagaccaag tttcggcggt
     9361 cttgggctgc ttctggatgt gaataggggt catattggtt tggtgcctga ggcattccag
     9421 ccagcaaggc taactgaggt aaacttaaat tattgaggtc tttaccatag tagttttgag
     9481 ctgctgtctg cattccatag ttcccattag acatgtagac cttatttata tagtaggtca
     9541 agatttcttg cttggttgct ttttgttcta actgaatcgc taaccaagct tcctgagcct
     9601 tacgagaaat agtctggtcg gaagtcgaag ttgaaaagta agtcaactta atcaactgtt
     9661 gagtgagagt tgatccacct tggagggaat tgctttgcag attgcgcaag aaagctccca
     9721 ggatacggat ggtatcaatc cccctgtggt cgaagaagcg atggtcttcg atagaaacga
     9781 ttgctttaac caaatctgtg ggaatatcat tagcttgggc attgacgcgg cgttcagaac
     9841 ccaagtcagc aatgagttga tttttattgt cgtagatttt actagaagtt gttgcaacta
     9901 gtttactctc ggataggcta ggagccttgc taacgtagta gaaaaaaact cctccgccta
     9961 agacaatggc tgcgataacc aagcttaaga agctaatgct cagatacttg attaggcgca
    10021 gaatcgttgg tttgttcatc ttgttttacc acctaataaa tgttctttga taacattgag
    10081 ataaggaatt tgagggaagg caccagcctt gatttcatat ccatattctc gaatatattc
    10141 aagtggcatt gatttttgtc ccttatcttg atgatagaag cgaatcaaat cgaatgccgg
    10201 caataagtag gtttcttgct gagaagaaaa gtgaagaagg acaaagcaga ttccttgttg
    10261 ggcaaggact tgttccatat gctgaatctg atgtggatga aaatttttca tcggaatcgc
    10321 acgtttttgt tttgtttcct tgacttcaaa gtcgatgtaa tatccattat aaacgccaga
    10381 atagtccgtc gttgaagctt gtcgaaaata ggcttcaaca atcttggcac gacttcgttg
    10441 tggatagtcc acttgtacga tttgaatagg agttggtttc ttatgtataa cagccaagcc
    10501 ctgagacaaa tagtagtcgt tggtagcatt gatcatcttt tcaaaagaca ttcctcgatt
    10561 tgcgaaattt ttgggttgag aaagagatgt ttgtcttttt tgtgatgaaa ctttatgtgg
    10621 atagttgatc ataattctcc ttattggtac aataacatca ctttattata ccataatttg
    10681 cacagaaagg gttaaaaatg gctacagctt tggttttagg ctattctgct tttgatttgg
    10741 gtttattttc tgataaggat cctcgtctta aattgattaa aaaagcgata cgtaaagatt
    10801 tagaggctat ggcagcagat ggggtgtctt ggcttgtgtt tacagggagt ttgggttttg
    10861 aatattgggt tttagaggtt gctcaggaga tgaaaaccga atacggtttc cagttggcca
    10921 ccatttttgc ttttgaaacc catggggaaa attggaatga aggcaatcag atgaaactga
    10981 gtcgctttaa gcaggtagac tttgtcaaat atgcttatcc tcgctatgaa cacaaggggc
    11041 agttaagaga ttaccagcag tttttgctgg aaaacacgac tagttcctat cttttttatg
    11101 atgaagaaaa tgaaacaaaa ctagcttatt tttaccaaaa gatgaaaaat caagaggact
    11161 attttataaa gagattaaca tttgatcaat taaatgaact tgctgaaaat ttttccgaaa
    11221 attgaagctt tgaccttgat ttttgtttgc ctttttttat ataataatac tagcaagcga
    11281 gaatggagag agacatggca agtattattt tttcagcgaa agatattttt gaacaagagt
    11341 ttggacgtga agtccgtggc tataataaag tagaagttga cgagttttta gacgatgtca
    11401 tcaaggacta tgaaacctat gctgccttgg tcaagtcact tcgtcaggaa attgcggatt
    11461 tgaaggaaga attaactcgt aaaccgaaac cttcaccagt tcaagcagaa ccccttgaag
    11521 cggcaattac aagttctatg acgaattttg atattttgaa acgcctgaat agattggaaa
    11581 aagaagtttt tggtaaacaa attttagata actcagattt ttaagtagtt atttgagatg
    11641 tgcaattttt ggataatcgc gtgaggagaa ttgcttctca tgaggaaagt ccatgctagc
    11701 acaggctgtg atgcctgtag tgtttgtgct aggcgaaacc ataagcctag ggacgagaaa
    11761 tcgttacggc agttgaaatg gctaagtcct tggataggcc agagtaggct tgaaagtgcc
    11821 acagtgacgg agtctttctg gaaacagaga gagtggaacg cggtaaaccc ctcaagctag
    11881 caacccaaat tttggtcggg gcatggagta tgcggaaacg aacgtagtat tctgactgct
    11941 atcagctaga gctgttagtg gtagacagat gattatcgaa ggaagtggtc ctagtcactt
    12001 ctggaacaaa acatggctta tagaaaattg catataggtt ggggctgaga aattttctca
    12061 acctcatttt ttaaagtgga catatagaaa ggtcttgcaa gactgtaaca tgaaaaaaga
    12121 atttaattta attgcaactg tggcagcagg gcttgaggct gtcgttggtc gtgaagtgcg
    12181 agagttgggc tacgattgtc aggttgaaaa tggacgtgtt cgttttcaag gagacgtgag
    12241 agctattatc gaaaccaacc tttggcttcg ggcagcagat cgtatcaaaa ttatcgtagg
    12301 aacgttccca gctaagactt ttgaagagct atttcaggga gttttcgctt tagattggga
    12361 aaattattta ccacttggag ctcggttacc gatttcaaaa gctaaatgtg ttaagtccaa
    12421 acttcacaat gagcccagtg ttcaggctat ttctaagaaa gctgttgtca agaaattgca
    12481 gaaacactat gctcgcccag aaggggttcc tctgatggag aatggcccag agtttaagat
    12541 tgaggtctct attctcaaag atgtggcaac tgtcatgatt gatacgaccg ggtctagcct
    12601 ctttaaacgt ggttatcgta ccgaaaaagg tggcgctcct atcaaggaaa atatggcagc
    12661 agccatttta caactttcta actggtatcc agacaagcct ttgattgatc cgacctgtgg
    12721 ttcggggact ttctgtattg aggcagttat gattgctaga aagatggcgc caggtcttcg
    12781 tcgctctttt gcatttgagg aatggaactg gatcagcgat cgcttgattc aagaagtgcg
    12841 cacagaagcg gctaaaaaag tagaacgtaa gcttgagttg gatatcatgg gctgtgatat
    12901 tgatgctcgc atggtggaaa ttgctaaggc caatgctcag gcagctggtg ttgcaggaga
    12961 tattactttt aagcagatgc gcgtgcagga tttacgttcc gataaaatca atggagtaat
    13021 catttccaat ccgccttatg gtgaacgttt gtcagatgat gcaggagtga ccaagctcta
    13081 tgctgagatg gggcaagtat ttgcaccgct gaaaacttgg agcaaattta tcctgactag
    13141 tgatgaagct tttgaaagca agtatggtag ccaagcagat aagaagcgta agttatacaa
    13201 cggaaccttg aaagtggatc tatatcaata ttttggtcag cgtgtcaaac ggcaagaggt
    13261 aaaatagaaa gggatactca tgagtaaaaa aagacgaaat cgtcataaaa aagaaggtca
    13321 agaaccgcaa tttgattttg atgaagcaaa agagctaaca gttggtcaag ctattcgtaa
    13381 aaatgaagaa gtggaagcag gagtcttgcc tgaggattcc attttggaca agtatgttaa
    13441 gcaacacaga gatgaaattg aggcggataa gtttgcgact cgtcaataca aaaaagagga
    13501 gttcgttgaa actcagagtc tggatgattt aattcaagag atgcgtgagg ctgtagagaa
    13561 gtcagaagct tcttcggagg aagttccatc ttctgaagac atcttactac ccttgcctct
    13621 ggacgatgag gagcaaggct tggatcctct attgctagat gatgaaaatc caacagaaat
    13681 gactgaagaa gtggaagagg agcaaaacct ttctcgtctg gatcaagagg actcagaaaa
    13741 gaaaagtaaa aaaggcttta ttttgaccgt tttggcgctt gtatcagtaa ttatttgtgt
    13801 cagtgcttat tatgtctatc gtcaagtggc tcgttcgacc aaggaaattg aaacttctca
    13861 atcaactaca gccaatcaat cggatgtgga tgattttaat acactttatg acgcctttta
    13921 cacaaatagc aataaaacgg ctttgaaaaa tagccagttt gataaactga gtcaactcaa
    13981 gactttactt gataagctgg aaggtagtcg tgaacatacg cttgccaaat ctaaatatga
    14041 tagtctagta acgcaaatca aggccattca agatgtcaat gcacaatttg aaaaaccagc
    14101 tattgtggat ggtgtgttgg ataccaatgc caaagccaaa tcggatgcta aatttacgga
    14161 tattaaaact ggaaatacgg agcttgataa agtgctagat aaggctatca gtcttggtaa
    14221 gagccagcaa acaagtactt ctagctcaag ttcaagtcaa gcaagttcaa atacgactag
    14281 tgagccaaaa ccaagtagtt caaatgagac tagaagtagt cgcagtgaag tcaatatggg
    14341 tctctcgagt gcaggggttg ctgttcaaag aagtgccagt cgtgttgcct ataatcagtc
    14401 tgctattgat gatagtaata actctgcctg ggattttgcg gatggtgttt tggaacaaat
    14461 tctagcaact tcacgttcac gtggctatat cactggagac caatatatcc ttgaacgtgt
    14521 caatatcgtt aacggcaatg gttattacaa cctctataag ccagatggaa cctatctctt
    14581 tacccttaac tgtaagacag gctactttgt cggaaatggc gctggtcatg cggatgactt
    14641 ggactactaa gcagtcgtta caaaattctt tcttttcaaa agtaaaaatg ataaaataaa
    14701 acaaatgaaa caagaggagt gtcaaatgac aaaagctaac tttggtgtcg taggtatggc
    14761 cgtaatgggt cgtaaccttg cccttaatat tgaatcacgt ggttacacag ttgctatcta
    14821 caaccgtagt aaagaaaaaa cggaagatgt gattgcttgc catcctgaaa agaattttgt
    14881 accaagctat gacgttgaaa gttttgtaaa ctcaatcgaa aaacctcgtc gtatcatgct
    14941 gatggttcaa gctggacctg gtacagatgc tactatccaa gcccttcttc cacaccttga
    15001 caaaggtgat atcttgattg acggaggaaa cactttctac aaagatacca tccgtcgtaa
    15061 tgaagaattg gcaaactcag gtatcaactt tatcggtact ggagtttctg gtggtgaaaa
    15121 aggtgccctt gaaggtcctt ctatcatgcc tggtggacaa aaagaggcct acgaattggt
    15181 ttcggatgtt cttgaagaaa tctcagctaa agcaccagaa gatggcaaac catgtgtgac
    15241 ttatatcggt cctgatggag ctggtcacta tgtgaaaatg gttcacaacg gtatcgagta
    15301 cggtgatatg caattgatcg cagaaagcta tgacttgatg caacacttgc taggcctttc
    15361 tgcagaggat atggctgaaa tctttactga gtggaacaag ggtgaattgg atagctactt
    15421 gatcgaaatc acagctgata tcttgagccg taaagacgat gaagaccaag atggaccaat
    15481 cgtagactac atccttgatg ctgcaggtaa caagggaact ggtaaatgga cgagccaatc
    15541 atctcttgac cttggtgtac cattgtcact gattactgag tcagtgtttg cacgctacat
    15601 ttcaacttac aaagaagaac gtgtacatgc tagcaaggtg cttccaaaac cagctgcctt
    15661 caactttgaa ggagacaagg ctgaattgat tgaaaaaatc cgtcaagccc tttacttctc
    15721 aaaaatcatt tcatacgcac aaggatttgc tcaattgcgt gtagcctcta aagaaaacaa
    15781 ctggaacttg ccatttgcag atatcgcatc tatctggcgt gatggctgta tcatccgttc
    15841 tcgtttcttg caaaagatta cagatgctta caaccgcgat gcagatcttg ccaaccttct
    15901 tttggacgag tacttcttgg atgttactgc taagtatcaa caagcagtgc gtgatatcgt
    15961 agctcttgcg gttcaagctg gtgtgccagt gccaactttc tcagcagcta ttacttactt
    16021 tgatagctac cgttcagctg accttccggc taacttgatc caagcacaac gtgactactt
    16081 tggtgctcac acttaccaac gtaaagacaa agaaggaacc ttccactact cttggtatga
    16141 cgaaaaataa gtaggtcagc catggggaaa cggattttat tacttgagaa agaacgaaat
    16201 ctagctcatt ttttaagttt ggaactccag aaagagcagt atcgggttga tctggtagag
    16261 gaggggcaaa aagccctttc catggctctt cagacagact atgatttgat tttattgaac
    16321 gttaatctgg gagatatgat ggctcaggat tttgcagaaa aattgagtcg aactaaacct
    16381 gcctcagtca tcatgatttt agatcattgg gaagacttgc aagaagagct ggaagttgtt
    16441 cagcgttttg cagtttcata catctataag ccagtcttta tcgaaaatct ggtagcgcgt
    16501 atttcggcga tcttccgagg tcgggacttc attgatcaac actgcagtct gatgaaagtt
    16561 ccaaggacct accgcaatct taggatagat gttgaacatc acacggttta tcgtggtgaa
    16621 gagatgattg ctctgacccg ccgtgagtat gaccttttgg cgacacttat gggaagcaag
    16681 aaagtattga ctcgtgagca attgttggaa agtgtttgga agtatgaaag tgcgaccgag
    16741 acaaatatcg tagatgtcta tatccgctat ctacggagca agcttgatgt taaaggacaa
    16801 aaaagctaca ttaaaaccgt gcgtggtgtt ggttacacca tgcaagaata gaaaagcagt
    16861 tgcagttgtg taactgcttt tttgaggagt ttttatatat tgacatgcag tcgtgtcttt
    16921 gctacaatcg ttttaggagg atatataaat gaaacttttg aaaaaaatga tgcaagttct
    16981 actagcagtc tttttctttg gtttgctagc tacaaatacg gtatttgcga ataccacagg
    17041 tggccgattt gttgataagg ataatagaaa atattatgta aaagatgatc ataaagcaat
    17101 ctattggcat aaaatagacg gtaaaactta ctattttggt gatattggag agatggttgt
    17161 cggttggcaa tacttagaaa ttcctggaac aggttatcgt gatgatttat ttgataatcg
    17221 tccagtcttc gaaattggcc ttcaggagaa gtggtactat tttggacaag atggtgcttt
    17281 gctagaacaa acagataaac aagtactaga ggcaaaaacg tctgaaaata caggaaaagt
    17341 atacggtgaa caatatcctc tatctgctga aaagagaact tattattttg ataataatta
    17401 tgctgtaaag acaggctgga tttatgaaga cggcaattgg tattatttaa ataagctagg
    17461 aaattttggt gatgattctt acaatccact accaattggt gaagttgcta agggttggac
    17521 tcaagatttt catgttacta ttgacattga tagaagcaaa cctgctccat ggtactacct
    17581 ccgttcctca ggagcaatgg ccactggctg gtatcaggaa ggtaccactt ggtattattt
    17641 agaccagcca aatggcgata tgaaaacagg ttggcaaaac cttgggaaca aatggtacta
    17701 tctccgttca tcaggagcta tggcaactgg ttggtatcaa gatggttcaa cttggtacta
    17761 cctaaatgca ggtaatggag acatgaagac aggttggttc caggtcaatg gcaactggta
    17821 ctatgcttat agctcaggtg ctttggcagt gaatacgacc gtagatggct attctgtcaa
    17881 ctataatggc gaatgggttc ggtaatgaca gggcgcttat aattatatta gaacactcgc
    17941 ttaatcaaag aagtgacttt gttgtacttg acctgacgat tggggagatt aaaagcatgt
    18001 gaaatctaca catgcttttt cgttagttat gagtgttgaa cttgactaac cattgacaaa
    18061 agataagagc aatcaaaata agatacaaaa atatcaatgc taactgcacc gaaacgcccg
    18121 atgctatgga tagggctaat agggagagca gggacggggc gagaatgtct actgtgctca
    18181 gagcagagcc caccgtcccc atcttgtcac tggggatatg ggcaaagaca gctgcttgta
    18241 ggcgagggct gagaatgccg ataatcgtag aactgatgaa actggtcacg aaaattacga
    18301 tataggcttg acaaaggata ctagttatta gagccaatag ggaaatctca cagaaaacca
    18361 agaggtgcgt gaaacggata tttttaaata gattactgct ggtcatattg ccaaggatag
    18421 ctccacaaga catgatgatg gagagcaggg cgaagagttg cccaatgctc agttgtgcta
    18481 gtgtctgttg gatgaagcgg agggcaatca gccgagggga cacatccaag atggcaatca
    18541 tactggtcgt cagaaacagt agtatgacgg tatcttttaa cctcaggagc acagccagcg
    18601 actgtttaag atgttgaaaa tatttcttaa aactcagtgc tgtatgtgac atttcaatcc
    18661 ttttttcaac ctcatacaag gtatgtcgaa taacatacag gcccaaaaag gcaatgacaa
    18721 aagtcagagt gttgataagg gaaatagttt gaatacttat aacattgata aggaatgcgc
    18781 cagccagatt ggcgacaatg tggacaaccc tcatcagaga ctgcctgaac cccatggcat
    18841 catgcaggtc atccttaatt acactgatgt agagggcgtt catcatgtag gcagacaggt
    18901 agctcaaggt gtcagagatg aggttgatga tgacaatcat aatgactgaa actaatgaaa
    18961 agggcgacaa gacgagaaat actgtcaaaa tagcgtagag caggattttg attttggcaa
    19021 tacagagtgc gtgtttgact ctattttctt gaaaatctcc tagaatcccg agaaagagtt
    19081 gcgatagtag gggaaccatc tctgaaatgg aaatcagtaa aatcgctagc ggagcgaaat
    19141 tgtactgagc cacataattg ataatggcga ggtagaaaat actgtcgccg aatgatgaaa
    19201 aacagctgtt tagcagcaac tgtctatata tgttgtttgt ccaaaataat ttcatacatt
    19261 tctccttatg tttgcacatc gaatgggaat ttattattta atagacacca atataccccc
    19321 tgcttcctat tccgaagtca aacaaatctg ctttaccact atctccttga aacatcagta
    19381 aaccgtgatc atctcgttca gaacatgcta aaattacctg ttggattttt ttcatcagta
    19441 atttgttttg attgtagaca gtggtttgta gtaagctagt tattccgtgg caatatcctg
    19501 tcatcatttt tagatgatgg ttaaaaacaa attcttgata tttactaata atatctttgt
    19561 ttccgtcaca atcgtacata caaagatata agattattcc ggaaattcct tcgcaccaag
    19621 aaagttgtaa atttcctaaa ttttcagtat tagaagtaac tttttctaat aattttttta
    19681 attcagcatg gaatgtatgg agatcattat agaacataga aggttctaag actttagaat
    19741 aggcaaataa agcatatgca atttcacaat atccatgagc aaagctatag tctaaaatgc
    19801 tttctttggc tgtatctctt tgtaggaaat aatgataaat ttccccatat actattgtgg
    19861 atgaaagttt taagagtatt gtcattggta aattgatagt attttattaa tgataatagg
    19921 acaccagatt ttcccaatgc aaagtctatt tcttctagag tatcataatt ctctattaat
    19981 ttcttagcgg tgacgtttgc taattgtaga tagtattgat ttttactgat agaaaataaa
    20041 tgtaggagta accatagata gcctgcatca ccaaatagca gtgagtattg atattcatat
    20101 gtgtgtcgtt ctaattgtca ctgccttctt gatgatagag agtattttag aatccagttc
    20161 tggggggtac aatttattta gaggaaacag tagtccagct attccgtgtt ggaatgataa
    20221 attacttacg aaatttccaa attctgttga agaagtgatc cttccttcag aatttacagt
    20281 tttatctagt aaaaatcgta gaacactatt acattctgtt ttgtaatcat atttatgttc
    20341 ctctattttt ttaattttga ctaaattatt tgtttgactt aaatttagta gacattgtaa
    20401 ccaaaatagt tgctcatctg tgagctgacc ttgagtatgt gatagttcta gtaattgaat
    20461 aggatccttt agttagattc aatcaatcta taacacaaca acataataat acttttagga
    20521 taacttgtca aatattatag taaaataaaa taa
//
LOCUS       DAVBGY010000039        20508 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVBGY010000039 DAVBGY010000000
VERSION     DAVBGY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.039"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            208..621
                     /gene="rpsL"
                     /locus_tag="VQ620_001391"
     CDS             208..621
                     /gene="rpsL"
                     /locus_tag="VQ620_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET2763998.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            641..1111
                     /gene="rpsG"
                     /locus_tag="VQ620_001392"
     CDS             641..1111
                     /gene="rpsG"
                     /locus_tag="VQ620_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET2763999.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            1536..3617
                     /gene="fusA"
                     /locus_tag="VQ620_001393"
     CDS             1536..3617
                     /gene="fusA"
                     /locus_tag="VQ620_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET2764000.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            3721..8112
                     /locus_tag="VQ620_001394"
     CDS             3721..8112
                     /locus_tag="VQ620_001394"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HET2764001.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFQTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPVEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GDRRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPILATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNEIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNDYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            8214..8477
                     /locus_tag="VQ620_001395"
     CDS             8214..8477
                     /locus_tag="VQ620_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HET2764002.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            8470..8748
                     /locus_tag="VQ620_001396"
     CDS             8470..8748
                     /locus_tag="VQ620_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HET2764003.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            8769..8921
                     /locus_tag="VQ620_001397"
     CDS             8769..8921
                     /locus_tag="VQ620_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764004.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            8943..10184
                     /locus_tag="VQ620_001398"
     CDS             8943..10184
                     /locus_tag="VQ620_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET2764005.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENVGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            10194..10424
                     /locus_tag="VQ620_001399"
     CDS             10194..10424
                     /locus_tag="VQ620_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET2764006.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            10635..11357
                     /locus_tag="VQ620_001400"
     CDS             10635..11357
                     /locus_tag="VQ620_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2764007.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVMDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            11574..12908
                     /gene="pepC"
                     /locus_tag="VQ620_001401"
     CDS             11574..12908
                     /gene="pepC"
                     /locus_tag="VQ620_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HET2764008.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAK"
     gene            complement(12964..13875)
                     /locus_tag="VQ620_001402"
     CDS             complement(12964..13875)
                     /locus_tag="VQ620_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2764009.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(13899..14702)
                     /locus_tag="VQ620_001403"
     CDS             complement(13899..14702)
                     /locus_tag="VQ620_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HET2764010.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(14730..15719)
                     /gene="manX"
                     /locus_tag="VQ620_001404"
     CDS             complement(14730..15719)
                     /gene="manX"
                     /locus_tag="VQ620_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HET2764011.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(15952..16971)
                     /gene="adhP"
                     /locus_tag="VQ620_001405"
     CDS             complement(15952..16971)
                     /gene="adhP"
                     /locus_tag="VQ620_001405"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HET2764012.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKNLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(17310..18122)
                     /locus_tag="VQ620_001406"
     CDS             complement(17310..18122)
                     /locus_tag="VQ620_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2764013.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            18312..19730
                     /locus_tag="VQ620_001407"
     CDS             18312..19730
                     /locus_tag="VQ620_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET2764014.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNDENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            19783..20490
                     /locus_tag="VQ620_001408"
     CDS             19783..20490
                     /locus_tag="VQ620_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2764015.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLVLSYLVIICSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
BASE COUNT         5972 a         4021 c         4670 g         5845 t
ORIGIN      
        1 gaaattgatc tctaacatta ccaataaaaa tgaaaaatat cttgacaagc aagggaaaat
       61 tttgttacaa taatagacgg tactttttac ttttggtctc tcaagagtgt acagggacgt
      121 gctgacaaat gttgcaaaag tacacacaga tgatatctgt caccaagtgt atcatcacca
      181 aaaataaaaa aacacaggag aatgtagatg cctacaatta accaattggt tcgcaaaccg
      241 cgtaaatcaa aagtagaaaa atctaaatca ccagctttga acgttggtta caatagtcat
      301 aaaaaagttc aaacaaacgt ttcttcacca caaaaacgtg gtgttgcaac tcgtgttgga
      361 acaatgacac ctaaaaaacc taactcagcc cttcgtaaat tcgctcgtgt acgtttgagc
      421 aaccttatcg aagttactgc ctacatccca ggtatcggac acaacttgca agagcacagc
      481 gtggtgcttc ttcgcggtgg acgtgtaaaa gaccttccag gggtacgtta ccatatcgtc
      541 cgtggtgcac ttgatactgc aggtgttaac gatcgtaaac aaggccgttc taaatacggt
      601 actaaacgtc caaaagcata aggaaagggg ataaagagaa atgagtcgta aaaatagagc
      661 tccaaaacgt gacgtattgc cagatccgct atacaattca caactagtta ctcgtcttat
      721 caaccgcgtt atgcttgatg gtaaacgtgg tactgctgct tcaatcgttt acggtgcttt
      781 tgagcaaatc aaagaagcta ctggcaacga tgcacttgaa gtatttgaaa cagctatgga
      841 aaacatcatg cctgtacttg aagtacgtgc acgtcgtgtt ggtggttcta actaccaagt
      901 cccagttgaa gttcgtccag aacgtcgtac aacacttgga cttcgttggt tggtaacaat
      961 cgctcgtctt cgtggtgaac acacaatgca agaccgtctt gcaaaagaaa tcttggatgc
     1021 tgctaacaac actggtgcag ctgttaagaa acgtgaagat actcaccgta tggctgaagc
     1081 taaccgtgca ttcgcacact tccgttggta agataggatg cgaaagcgtt aagaaagtcc
     1141 cagagaaaat agggaatcga agcaggttgc ggttgcaacc aatgagattc atctttttct
     1201 ccagactttt agcttgagct caactaaatc atgatgctag gcacggtaag gatgcaaggt
     1261 gaaaatagga aactgacgca gtattcaacg aatacaagga gttttatctt tttcacgcag
     1321 catcccgttc cagctcacat cggctaacta actttagccc gggttcaaat tagctaaatc
     1381 gattagtatt agctataact cagcttacca tctcgtaagt tgaaaccaac aatagcatga
     1441 aaacattgag aacgggtagg tcctgcctat ccgtttttat taaaatcgtg ttataataga
     1501 atagaaatca aaaataaata ggagaaacaa acctcatggc acgcgaattt tcacttgaaa
     1561 aaactcgtaa tatcggtatc atggctcacg tcgatgccgg taaaacaaca actactgagc
     1621 gtattcttta ctacactggt aaaatccaca aaatcggtga aactcacgaa ggtgcgtcac
     1681 aaatggactg gatggagcaa gagcaagaac gtggtatcac gatcacatct gctgcgacaa
     1741 cagctcaatg gaacaaccac cgcgtaaaca tcatcgacac accaggacac gtggacttca
     1801 caatcgaagt acaacgttct cttcgtgtat tggatggtgc ggttaccgtt cttgactcac
     1861 aatcaggtgt tgaacctcaa actgaaacag tttggcgtca agcaactgag tacggagttc
     1921 cacgtatcgt atttgccaac aaaatggaca aaatcggtgc tgacttcctt tactctgtaa
     1981 gcacacttca cgatcgtctt caagcaaatg cacacccaat ccaattgcca atcggttctg
     2041 aagatgactt ccgtggtatc attgacttga tcaagatgaa agctgaaatc tatactaacg
     2101 accttggtac ggatatcctt gaagaagaca tcccagctga ataccttgac caagctcaag
     2161 aataccgtga aaaattgatt gaagcagttg ctgaaactga cgaagaattg atgatgaaat
     2221 acctcgaagg tgaagaaatc actaacgaag aattgaaagc tggtatccgt aaagcgacta
     2281 tcaacgttga attcttccca gtattatgtg gttcagcctt caaaaacaaa ggtgttcaat
     2341 tgatgcttga tgcggttatc gactaccttc caagtccact tgacatccca gcaatcaaag
     2401 gtattaaccc agatacagac gctgaagaaa ctcgtccagc atctgacgaa gagccatttg
     2461 cagctcttgc cttcaagatc atgactgacc cattcgtagg tcgtttgaca ttcttccgtg
     2521 tttactcagg tgttcttcaa tcaggttcat acgtattgaa tacttctaaa ggtaaacgtg
     2581 aacgtatcgg acgtatcctt caaatgcacg ctaacagccg tcaagaaatc gacactgttt
     2641 actcaggtga tatcgctgct gccgttggtt tgaaagatac tacaactggt gactcattga
     2701 cagatgaaaa atctaaaatc atccttgagt caatcaacgt tccagaacca gttatccaat
     2761 tgatggttga gccaaaatct aaagctgacc aagacaagat gggtatcgcc cttcaaaaat
     2821 tggctgaaga agatccaaca ttccgcgttg aaacaaacgt tgaaactggt gaaacagtta
     2881 tctcaggtat gggtgaactt caccttgacg tccttgttga tcgtatgcgt cgtgagttca
     2941 aagttgaagc gaacgtaggt gcgcctcaag tatcttaccg tgaaacattc cgcgcttcta
     3001 ctcaagcacg tggattcttc aaacgtcagt ctggtggtaa aggtcaattc ggtgatgtgt
     3061 ggattgaatt tactccaaac gaagaaggta aaggattcga attcgaaaac gcaatcgtcg
     3121 gtggtgtggt tcctcgtgaa tttatcccag cggttgaaaa aggtttggta gaatctatgg
     3181 ctaacggtgt tcttgcaggt tacccaatgg ttgacgttaa agctaagctt tatgatggtt
     3241 catatcacga tgtcgactca tctgaaactg ccttcaagat tgcggcttca ctttccctta
     3301 aagaagctgc taaatcagca caaccagcta tccttgaacc aatgatgctt gtaacaatca
     3361 ctgttccaga agaaaacctt ggtgatgtta tgggtcacgt aactgctcgt cgtggacgtg
     3421 tagatggtat ggaagcacac ggtaacagcc aaatcgttcg tgcttacgtt ccacttgctg
     3481 aaatgttcgg ttacgcaaca gttcttcgtt ctgcatctca aggacgtggt acattcatga
     3541 tggtatttga ccactacgaa gatgtaccta agtcagtaca agaagaaatt attaagaaaa
     3601 ataaaggtga agactaatcc gtcctcactc tagaaggaag tcacttagtg gcttcctttt
     3661 gtctttagaa aatacctcta aatatggtaa aatagtagaa gaataatgtg aggaaaatga
     3721 atgtcaaata gttttgaaat tttgatgaat caattgggga tgcctgctga aatgagacag
     3781 gctcctgctt tagcacaggc caatattgag cgagttgtgg ttcataaaat tagtaaggta
     3841 tgggagtttc atttcgtatt ttctaatatt ttaccgattg aaatcttttt agaattaaag
     3901 aaaggtttga gcgaagaatt ttctaagaca ggcaataaag ctgtttttga aattaaggct
     3961 cggtctcaag aattttcaaa tcagctcttg cagtcctact atagggaggc tttctctgaa
     4021 ggtccatgtg ctagtcaagg ttttaagtcc ctttatcaaa atttgcaagt tcgtgctgag
     4081 ggtaatcagc tatttattga aggatctgaa gcgattgata aggaacattt taagaagaat
     4141 catcttccta atttagccaa acaacttgaa aagtttggtt ttcaaacttt taactgtcaa
     4201 gtcgagaaga atgatgtcct gacccaagag caggaagagg cctttcatgc tgaaaatgag
     4261 cagattgttc aagctgccaa tgaggaagcg ctccgtgcta tggaacaact ggagcagatg
     4321 gcacctcctc cagtggaaga gaaaccagtc tttgattttc aagcgaaaaa agctgcagct
     4381 aaacccaaac tggataaggc ggagattact cctatgatcg aagtgacgac agaggaaaat
     4441 cgtctggtat ttgaaggggt tgtttttgat gtggagcaaa aagtgactag aacaggtcgt
     4501 gttttaatca actttaaaat gacggactat acttcaagtt tttctatgca aaagtgggtt
     4561 aaaaacgagg aagaggccca gaagtttgac ctcatcaaga agaattcttg gctccgagtt
     4621 cgagggaatg tggagatgaa taacttcaca cgcgatttga ctatgaacgt acaggatctg
     4681 caggaagttg ttcactatga gcggaaggat ttgatgccag aaggtgatcg tcgggttgag
     4741 tttcatgctc atactaacat gtcgactatg gatgctttgc cagaggtcga agagattgtt
     4801 gcaacagctg ctaagtgggg acacaaggcg gttgctatca cagaccatgg gaatgtccag
     4861 tcctttccac atggctataa ggcggctaag aaagcgggaa tccagctgat ctatgggatg
     4921 gaagccaata tcgtggagga ccgtgtccct atcgtctata acgaagtgga gatggactta
     4981 tcagaagcaa cctacgtggt ctttgacgtg gaaacgacgg gactttcagc tatctataat
     5041 gacttgattc aggttgcggc ctctaagatg tacaagggga atgttattgc tgaatttgat
     5101 gaatttatca atcctgggca tcccttgtca gcttttacta cagagttaac tggaattaca
     5161 gatgatcatg tcaaaaatgc caaaccacta gaacaagttt tgcaagaatt ccaagaattt
     5221 tgcaaggata cggtcctagt tgcccacaat gctacctttg acgttggctt tatgaatgct
     5281 aattatgagc ggcatgacct tccaaagatt agtcagccag ttattgatac gctggagttt
     5341 gctagaaacc tctatcctga gtataaacgc catggtttgg ggcctttgac caagcgtttt
     5401 ggtgtggcct tggaacatca ccacatggcc aactacgatg cggaagcgac tggtcgtctg
     5461 cttttcatct ttatcaaaga ggtagcagaa aaacatggtg tgaccgattt agctagactc
     5521 aacattgatc taatcagtcc agattcttac aaaaaagctc ggatcaagca tgcgaccatc
     5581 tatgtcaaga atcaggtagg tctaaaaaat atctttaagc tggtttcctt gtctaatacc
     5641 aagtattttg aaggagtgcc acggattccg agaacggttc tagatgccca tcgagagggc
     5701 ttgattttag gttcagcctg ttcagagggt gaagtttttg acgtggtcgt ttctcaaggt
     5761 gtggatgcgg cggttgaggt ggccaagtat tatgatttta tcgaggtcat gccaccggct
     5821 atctatgcac ccttgattgc caaagagcag gtcaaggata tggaggaact ccagaccatt
     5881 atcaagagtt tgatagaggt tggagaccgc cttggcaagc ctattctggc tacgggaaat
     5941 gttcactata tcgaaccgga agaagagatt tatcgtgaaa ttatcgtccg tagtttggga
     6001 cagggtgcga tgattaatcg aactatcggt catggtgaac atgcccaacc agcaccactt
     6061 ccaaaggctc attttcgaac gactaatgag atgttggatg aatttgcctt tttgggagag
     6121 gaactggctc gtaaactggt tattgaaaac accaatgcct tggcagaaat atttgaaccc
     6181 gttgaagtcg ttaagggtga cttgtatacg cctttcatcg acaaggctga agaaacagtt
     6241 gctgagttga cctataagaa agcttttgag atttatggaa atccgctgcc agatattgtt
     6301 gatttgcgga ttgaaaaaga attaacatcc atactgggga atggatttgc tgtgatttat
     6361 ctggcatcgc agatgctggt gcaacgttct aatgaacggg gttatttggt tggttctcgt
     6421 gggtctgtcg gatctagttt cgttgcgacc atgattggga ttacggaggt caatcctctc
     6481 tctcctcact atgtctgtgg tcagtgtcag tacagtgagt ttatcacaga tggttcgtac
     6541 ggttcaggat ttgatatgcc ccataaggac tgtccaaact gtggtcacaa actcagtaaa
     6601 aacggacagg atattccgtt tgagaccttc cttggttttg atggggataa ggttcctgat
     6661 attgacttga acttctcggg agaagatcag cctagcgccc acttggatgt gcgtgatatc
     6721 tttggtgaag aatatgcctt ccgtgcggga acggttggta cggtagctgc caagactgcc
     6781 tatggatttg tcaagggtta cgagcgagat tatggcaagt tttatcgtga tgcagaggtg
     6841 gaacgcctcg ctcaaggagc ggcgggtgtc aagcggacaa caggccaaca cccgggggga
     6901 atcgttgtta ttccgaacta catggatgtc tacgatttta cgcctgtcca gtatccagca
     6961 gatgatgtca cggctgaatg gcagaccact cactttaact tccacgatat cgatgagaac
     7021 gtcctcaaac tcgatgtact gggacatgat gatccgacca tgattcgaaa acttcaggat
     7081 ttgtctggta ttgaccctaa tgaaattcct atggatgacg aaggcgtgat ggcactcttt
     7141 tctgggactg atgtgctagg ggtaacacct gaacaaattg gaacgcctac gggtatgttg
     7201 gggattccag agtttggaac aaatttcgta cgtggaatgg tagacgaaac ccatccgaca
     7261 acctttgcgg aattgcttca gctgtctggt ctgtcacacg gtactgacgt ttggttgggg
     7321 aatgctcagg atctgattaa gcaaggaata gcggacctat cgactgttat cggttgtcgg
     7381 gacgacatca tggtttacct catgcatgcg ggtctggaac ctaagatggc ctttaccatt
     7441 atggaacggg tacgtaaggg tttgtggcta aagatttcag aagaggagag aaatgactat
     7501 atcgaagcca tgaaggctaa taaggtgcca gagtggtata tcgaatcctg tgggaaaatt
     7561 aagtacatgt tccctaaggc ccatgcggca gcctacgtta tgatggcctt gcgtgtagct
     7621 tacttcaagg ttcaccatcc tatttattac tactgtgctt acttctccat tcgtgctaag
     7681 gcttttgata tcaagaccat gggtgcgggc ttggaggtca tcaagcgcag aatggaagaa
     7741 atctctgaaa aacggaagaa caatgaagcc tctaatgtgg aaatcgatct ctatacaact
     7801 cttgagattg tcaatgagat gtgggaacga ggtttcaagt ttggtaaatt agatctctac
     7861 cgtagtcagg cgacagagtt cctcatcgac ggggataccc ttatcccacc atttgtagca
     7921 atggatggtc tgggagagaa cgttgccaag caactggtgc gggcgcgtga agagggagaa
     7981 ttcctctcta aaacagaact acgcaagcgt ggtggactct catcaacctt ggttgaaaag
     8041 atggatgaga tgggtattct tggaaatatg ccagaggata accagttgag tttgtttgat
     8101 gagttgtttt aaaaaattgc ttaataatct attaaaagag gctaacgtat atccaataga
     8161 tttacattag ctttcttttt tgttaaaata gtctatggaa atagggtgag agtatgtcaa
     8221 agatgagtat aagcatccgt ctggatagtg aggttaagga gcaggcccaa caggtgttta
     8281 gtaatctggg aatggatatg acaacagcta ttaatatttt ccttcgtcag gcaattcaat
     8341 atcagggatt accttttgat gttagactag acgaaaatcg gaagttgctc caagtgttaa
     8401 cagatttaga ccaaaatcgt aatatgagcc agtcttttga atcagtctca gatttgatgg
     8461 aggacttacg tgcttaagat tcgttatcat aaacagttta aaaaagattt taagttggct
     8521 atgaagcgtg gtttgaaggc cgaattatta gaagaagttt tgaattttct ggttcaagaa
     8581 aaagaacatc ctgccagata tcgtgatcat tcattgacgg catccaagca ttttcaagga
     8641 gttcgtgaat gccataccca gccagattgg cttttggttt ataaagtaga caagtcggaa
     8701 ttgattttaa atttgctgag gacaggcagt cacagtgatt tattttaatc tattttaagg
     8761 gggttctcat gaaactaaga atatttgcgg aagataagcc ggctaagaag gtatttgaat
     8821 atcaattaga acttgctgat cgtacaattc ttctatcgac agcactcttg tcaggtgcta
     8881 ttgctttagc aggaatcttt tctgccttga aagaaaaata aaaatagaaa agagaaaaca
     8941 gaatggtttt accaaatttt aaagaaaatc tagaaaaata tgcgaaattg ttggttgcga
     9001 acggaattaa cgtgcaacct ggtcacactt tggctctctc tattgatgtg gagcaacgtg
     9061 aattggcaca tctaatcgtg aaagaagctt atgccttggg tgcgcatgag gtcatcgttc
     9121 agtggacaga tgatgtcatt aaccgtgaga aattcctcca tgccccgatg gagcgtttgg
     9181 acaatgtgcc agaatacaag attgctgaga tgaactatct tttggagaac aaggctagcc
     9241 gtcttggggt tcgttcatct gatccaggtg ccttgaacgg agtggacgct gacaagcttt
     9301 cagcttctgc taaagctatg ggacttgcca tgaagcctat gcgtatcgca actcaatcta
     9361 acaaggttag ctggactgta gcagctgcag caggacttga gtgggctaag aaagtcttcc
     9421 caaatgctgc gagcgacgaa gaagcagttg atttcctttg ggaccaaatt ttcaaaactt
     9481 gccgtgtcta tgaagcagat cctgttaagg cttgggagga acatgcagcc attctcaaga
     9541 gcaaggcaga tatgcttaat aaggagcaat tttcagccct tcactacaca gcgccaggaa
     9601 cagatttaac acttggtttg ccaaagaacc acgtttggga atcagctggt gctgtcaatg
     9661 cacagggcga aggattcttg ccaaatatgc cgacagaaga ggtcttcaca gcgcctgact
     9721 tccgtcgtgc agatggttat gtcacttcta caaaaccgct tagctacaac ggaaatatca
     9781 ttgaaggcat taaggtgacc tttaaggatg gacaaatcgt agatatcact gctgagaagg
     9841 gtgatcaggt tatgaaagac cttgtctttg aaaatgtggg tgcgcgtgcc ttgggtgaat
     9901 gtgccttggt accagatcca agtccaattt ctcagtcagg cattaccttc tttaacaccc
     9961 ttttcgatga aaatgcgtca aaccacttgg ctatcggtgc agcctatgcg actagcgttg
    10021 ttgatggagc ggagatgagc gaagaggagc ttgaagctgc agggcttaac cgttcagatg
    10081 ttcacgtaga ctttatgatt ggttctaacc aaatggatat cgatggtatt cgtgaggatg
    10141 gaacgcgagt acctcttttc cgtaatggga attgggcaaa ttaaggagat aatatgttag
    10201 gaagtatgtt cgttggtctc ctagtgggat ttttagcagg tgctatgacc aatcgtggag
    10261 agcgaatggg atgttttgga aaaatgtttc tcggttggat cggagccttt ctaggtcact
    10321 tgctctttgg aacttggggg ccagttttat caggaacagc tattatccca gcgattttag
    10381 gagccatgat tgttttagct attttttgga gacgaggaag ttaatttcct aaatctgata
    10441 ctcaatgaaa atcaaagagc aaactaggaa actagccgca ggttgctcaa agcactgctt
    10501 tgaggttgca gatagaactg acgaagtcag taacatatat acggcaaggc gacgttgacg
    10561 cggtttgaag agtatgaaac gaaaaggagg ttggtcattg taccagcctc cttttgatat
    10621 gatataatag ttctatgaga ttagataaat ttttagttgc ctgtgcggtg ggaagtcgga
    10681 ctgaggtcaa aaacttgctc aaggctgggc gcgtgactgt aaatggtaaa aaagaaaaat
    10741 cagctaaatt gcagattgat gaaaaaatag atgagattcg ctttgatggg caagtgttgg
    10801 agtatgaaga gtttgtctac tacatgatga acaagcccaa aggagttatc tcagcgactg
    10861 aggatcccaa gcacagaacc gttatggact tgttggatga cttggcgcgg agtaaggaag
    10921 ttttcccagt aggacgcttg gatattgaca cgcatggtct tttgctcttg accaatgatg
    10981 gtcagctggc tcatgttctt ctttcgccca agcgtcatgt ggacaagact tatctggcac
    11041 aagtcaaggg aatcatgacc caagaagatg tggagacatt tgctgagggt attcctctca
    11101 aagactttac ctgtcaaccc gctatactgg agcttgtatc catagataca gaaaagaatc
    11161 aaagccaaat ccgtgtgacc attgcagaag ggaagtttca tcagatcaag cgtatggtgg
    11221 gctactgtgg caaggaagtg gtggacttgc aacgattgac tatgggaact ttagtattag
    11281 atgagaacct ggaacgaggg gaatggcgtc gcttgaccaa ggaagaatta gaaattctcc
    11341 gcgctaatat tatttaattt agtttaatac tcaatgaaaa tcaaagagca aactaggaaa
    11401 ctagccgcag gctgtacttg agtacggcaa ggcgacgttg acgtggtttg aagagatttt
    11461 cgaagagtat aaattcgaaa aaggtgaggg acaatgtcct cgccttttat gttttttagt
    11521 tgcttccttt gtgaaaagag ttataataga ctgtagaata aaaggaggaa tctatgaacg
    11581 cgattcaaga atcatttact gataaactat ttgccaacta tgaagcaaat gtaaaatacc
    11641 aagcgattga aaatgctgcc agccacaacg gaatttttgc agctctggaa cgtcgccaaa
    11701 gccatgtaga caacacacct gttttctcat tggatttaac caaggacaag gtcactaacc
    11761 agaaagcgtc tggtcgttgc tggatgtttg cggctctcaa caccttccgc cacaaactca
    11821 tctcgcaata caaattggag aactttgagt tgtcacaagc ccacactttc ttctgggata
    11881 agtatgagaa atcaaactgg ttcttggagc aagtcattgc gacttcagac caagaattga
    11941 ctagccgcaa ggttagcttc ttactccaaa cacctcaaca agatggtggt caatgggata
    12001 tggtcgtttc cctctttgaa aaatacggtg tcgtgcctaa gtcagtttat cctgagtctg
    12061 tttcatctag cagcagtcgt gagctaaatg cgatccttaa taaattgctt cgtcaagatg
    12121 ctcaaatctt gcgtgatttg cttgtttctg gtgcagatca agtgactgtt caagctaaga
    12181 aagaagacct cttgcaagaa atctttaact ttcttgctat gtcattagga cttccaccac
    12241 gcaagtttga ctttgcttat cgcgataaag ataacaacta caaaagtgaa aaaggaatca
    12301 caccacaaga gttctacaag aaatatgtca atcttccttt agaagactac gtttctgtta
    12361 tcaatgctcc aactgctgat aaaccttacg gaaaatctta cacagttgag atgttgggga
    12421 atgtggttgg tagccgtgca gttcgttaca tcaacgttcc aatggagcgc ttgaaagaat
    12481 tggcgattgc ccaaatgcaa gcaggtgaga ctgtttggtt tggttctgat gtcggccagc
    12541 tcagtaaccg taaggctgga atccttgcga cagatgttta tgactttgaa tcaagcatgg
    12601 acattaaact tactcaagac aaggctggac gtttggacta tagcgaaagc ttgatgaccc
    12661 acgccatggt cttgacaggt gttgacttgg acgaaaatgg taaatcaacc aagtggaagg
    12721 ttgaaaactc atggggagac aaggtcggta cagatggtta ctttgttgcc tcagacgctt
    12781 ggatggacga atacacatac caaatcgttg ttcgtaagga attgctgaca gcagaagaac
    12841 aagctgccta tggagcagaa ccaattgtac ttgcaccatg ggatccaatg ggagccttgg
    12901 ctaaataaaa gcatagaaaa aaggaatcag attttagaac ctggttcctt tttagttgct
    12961 tgattacatg atgtgaagaa catgtgccac aatacccact gcgaagaggg caaggataat
    13021 agtgattgga gatacttttt tcttaagtaa gtacatgcaa agtaaagtaa ggagtagtcc
    13081 tgataatcca ggaatcaaca tatccaagtt ttgttggaaa gtagtaactt tttcaggagt
    13141 ttgagacaat ccttgtccta cttgtgcgaa tgcttcttgg atacctttag acccttctgg
    13201 caatttatcc caatggatat aagccttttc atctagttga actttagaaa catcgaaagc
    13261 aaatttaata tttacccagc gttgaacaag gacagcaaga atgaacatcc caaggataga
    13321 agctccttta gtgatatctt gaaggatacc accagacata tctttagtga tttctgatcc
    13381 agccttgtat ccaatctctt gaacatacca caagaatgac atacgaatca agttccatgc
    13441 aacaaagaag aggagtggcc ccaagatatt gccagtaagg gcaagtgaag caccgagaga
    13501 tccaaggatt gggcgtactg taaaccagaa tactgggtca ccgataccag caagaggtcc
    13561 catcataccg attttaaccc cttggatagc agcgtcatcg atttccacac cgttagcacg
    13621 ttcttcttca agcgcaagag taacccccat gactggagca gctacgtatg gatgagtgtt
    13681 gaagaactca aggtgacgct caagagcagc gatttgatct tcttttttag tatagagttt
    13741 tttgatagct ggaatgagtg tataagccca gcccaagttt tgcatccgtt caaagttcca
    13801 agacccttgt aagaaggttg aacgccacca aactttttta cgatctgatt tagttaattg
    13861 aagtttttca gtcatgatgt tttcagtcct ttcttatctt agtagtcttc taggatatcg
    13921 ccgattgggt cgttagaagt tgcggctcct ccgccaccat ttccaccagt tttagaaagg
    13981 tgaaggtaga taagagcgat agcaacaccg atagcaccga atccgattag agtaatatct
    14041 gacacagcag cgagaacgaa accaagagcg aagaatggcc atacttcacg agttgccatc
    14101 atgttgataa ccatggcgta accaacggca acgaccatac caccaccgat agccatacca
    14161 tctttgagcc agtctggcat ggcactaagg atactttgta cagtttcagt tggtaccata
    14221 aggagaagag ctgcaggaag cgcgatacga agtccttgga agagtagcgc aatgaaatgc
    14281 gcacgctcca cagcgccgaa gtcacctttt ttagcggcag catctgcagt atgaaccaaa
    14341 ccaactgaaa ttgtacgaac aatcattgtc aagaaaagtc cagctacggc aagagggata
    14401 gcaaccgctt gggcaacacc gataccagtc ttggtaaagt caccaccaag aaccatgata
    14461 atggcagcag cgacagaagc aagtgcagca tcaggagcga tagcagcacc gatatttgac
    14521 caaccaaggg caatcatttg aagcgatcca ccgaggataa tccctgcttc caagtgacct
    14581 gttacaagcc caataagggt acaggctaca agtggttggt gaaattggaa ctggtcgagg
    14641 atgccttcaa gacctgcaaa gaaggctaca acgactacta aaaccataga aataatagac
    14701 atgtttaaaa tcctttcata aataatggct tatttgacat tggctttgtt aatcaagtca
    14761 aacaaatctt ttttagaatc atttggtact ttacggacat caaattcaac acccaagtca
    14821 cgcatttttt caaatgtagc aacatcttct ttgtccatag acaaaacggt attgaccaat
    14881 gttttacctg ttgagtgagc catagaacca acgttaagag tcttgattgg cacgccgcct
    14941 tcgatggcac gaagggcatc ttgaggtgtt tcaaacaaga taagggcatg tgtttctcca
    15001 aaacgtgggt cttttgaaat ctcaatcagt ttttgaattg gaaccacgtt agccttgaca
    15061 ttacctggag ctgcttgttt aatcaattct ttacgaaggt cgtctttagc cacgttatct
    15121 gaagcaacga tgatacgatt tgcttttgaa tctggagtcc aagcagttgc aacctgaccg
    15181 tgaagtagac gtgtgtcaag acgggcaaga ttgattttca atttaccgtc tccgataaca
    15241 gttccttctg ggatagcagt ttgggcaact ggagcagctg cagcgcttgc aacttcttca
    15301 actggattta gctcttctgg aagagctttg atgccatctt tggcttcttt aatgatatta
    15361 gcagcgactt tttctacacc tgcagcagcg tccatgaggc gttctgtgta ggcttgaatc
    15421 aacatcggta agttaagtcc tgtgatgatg gcaaacttac gctcaggatt ttctcccatc
    15481 acgcgactag cttggttaaa tggagaacca ctccaaaggt cagccaaaac tagaacctca
    15541 tcttctgcgt caaatgcagc aacagcgtta ttaaacttag cgtatagatc atcaggacct
    15601 tcatttggca taaaggtcac aacttgaacc ttttcttgtt caccaaagat catagatcct
    15661 gactgatgaa tacccgcagc aaattcgccg tggctcgcaa taatgattcc gatactcatt
    15721 attgtcattc ctcctttttt gttttagttt tcatttaaga aaactttaag actgtttaag
    15781 tataaaccgt tttcataaaa aaagcaacta tttatcataa aaaattaaaa aatttttatt
    15841 tttgtatgtt gatatgttga atttttgagg agtaatcttg aattcttatt taaaatttaa
    15901 tataaaaaag ttggaagaag cttccaactt ttaaaatagg tttctattag attagtgggt
    15961 gaagtcgagt accatacgtc cttggatttg gcctttttcc atttcgtcga aaatggcaac
    16021 agcatcttct actggacgtt tttgaacaac tgggactacc agaccttctg caccaaattg
    16081 gaaggcttct tctaagtctt tacgagttcc gacaagagaa ccgatgactt ggattccatc
    16141 gaggactgtt ttaacgatgc tgagttccat catttcagaa ggaagaccaa cagcgacgac
    16201 gcgaccacca gcacgaatgg agtcaacagc ctggttgaag gcaactttag acacagcagt
    16261 tacgacagct gaatgagctc ctccatcagt tttttcttta atgagtccag ctacatcttc
    16321 aacttcgagg ccgttaatca caatgtcagc gcctacttct tttgcaaggg caagtttgtc
    16381 attgttgata tcgactgcga taacatgagc attgaatact tttttagcgt attgaacagc
    16441 gaggttacca agtccaccag caccgtaaag aacaacccat tggcctggtt caacttttgc
    16501 ttctttgata gctttatagg ttgttactcc agcacatgtg atagaagaag cttgggctgg
    16561 atcaagtccg tcaggaactt tgacagcata gtcagcagtt acgatacatt gttcagccat
    16621 accaccgtct actgagtagc cagcattttt cactgtacgg caaagggttt cgcgaccagt
    16681 tgtacagtat tcgcaagtgc cacatccttc aaagaaccaa gcaacgctga cgcggtcacc
    16741 gactttaagg tttttcacat ctggagcaat ctctttaacg ataccgatac cttcgtgccc
    16801 aagaacacgt cctgggactt gaccaaagtc accatgagca acgtggaggt cggtgtggca
    16861 aacgccacag tattcaactt ctacaagtgc ttccccagtt tcaagtggac ggagtacttt
    16921 ttcttcaata gcaacaccag tgctttctgg atttacaaca acagctttca tagctatcct
    16981 ccttgatatt gaccgagaac agggacaacg ggactggatt gattttttgt caacagagtc
    17041 gagtgtgacg agctctcttg tatttatatg tgaagtatat cacaaaagaa agccttttcc
    17101 aaggttttgg agacaaaaag tagaacaatc gattaactgg ttgatgagac tgtgaaaaga
    17161 gcacaaagac cagcttgcaa gctggtcata ctcttcgaaa atctcttcaa accacgtcag
    17221 cttcatctac aacctcaaag cagtgctttg agcaacctac ggctagcttc ctagtttgct
    17281 ctttgattct cattgagtat cagtataggt tataagaata agtcttgtag ggttttggca
    17341 accccatctt ggtcgttggt aagggaaatt tgctcatctg cataagggag tagctctgga
    17401 ttggcatttt tcatggcata acccttccca gcaaaagcga gcatttcggt atcattgtgt
    17461 tcatctccaa aggcaatcaa atcttttttg tcacgattca ttatcttgag caagtagtcc
    17521 aaagcaaagg ccttgttgac accttttggg gtacattcaa ggatattgag cggacctccc
    17581 caggtattga tagaaagttg atgctggtag aaggcgttca tttcttttgc caaggcatat
    17641 ttgtcactgg ctctggtctg caagaggata cagttagggt ccttggtcac caattcaggc
    17701 tggaattgat cttcaggctg gaaagcttct acaccaaata gtttgggatt ggcaatttct
    17761 tcattgggat ttgtaatgta gaatttttta cgatattctc cagcgataaa atcggcttga
    17821 atgtcctctg aacgttgaac catatctagc agatattttt tgtctacagt caaacacttt
    17881 tcaaaatccc aaacttggtc tggtaaatga gtaagggatc cgttgaagtt aatcatagga
    17941 gtgtctaagc ccagttcacg gtaaaaatct tttgacatac ggtaagggcg acctgtcgta
    18001 ataataacct gatggccttt ttcagcaact tttttgatgg tttttttggt aaagtcagaa
    18061 atttgactgt ctgagttgag cagggttcca tctaggtcaa ctgcaataat ttttttcgtc
    18121 atatggacct ttctagtgtg ttttcagata agatgtcttc tattataaca gaaagcaggc
    18181 agaaaagcag attccaaaca aaaaatgaaa ttggattaat ttcttaaaca aatcaaacaa
    18241 agacattgaa aaagtgaatg ataaatgata taattctatt attgttcgta aaaattaaaa
    18301 ggagattgat gatggacaaa ttatttaaac taaaagagaa cggtacagac gttcgtacag
    18361 aggttctcgc tggtttaaca actttctttg caatgagcta tattctcttt gtaaacccac
    18421 aaatactttc acaaacagga atgcctgctc agggtgtgtt tcttgcaacg attatcggtg
    18481 cagttgctgg taccttgatg atggccttct atgctaactt gccatacgct caagcaccag
    18541 gtatgggact caatgccttc tttaccttta cagtcgtatt tggacttggt tattcttggc
    18601 aagaagccct agctatggtc ttcatctgtg ggattatttc attgattatt accttgacaa
    18661 atgttcgtaa aatgatcatt gaatcgattc ccaatgctct tcgctcagct atttcagctg
    18721 gtatcggtgt cttccttgcc tatgtaggga ttaagaatgc tggacttttg aaattcacga
    18781 ttgatccagg caactatact gttgtaggag aaggggctga caaagctcaa gcaacgattg
    18841 cagcaaactc ttcagcagtt ccaggattgg tcagctttaa taatccagct gttttagtgg
    18901 ctcttgcagg acttgccatt actatcttct ttgtcatcaa agggattaaa gggggaatta
    18961 ttctctctat cttgacaaca actgttcttg ctattgcagt tggtttggtt gatttgtcta
    19021 gtatcgattt tgctaataac catgttggtg cagcttttga agatttgaag acaatctttg
    19081 gtgcagctct tggttcagaa ggtttgggag ctttggtttc agatacagct cgcttgcctg
    19141 aaactctgat ggccattctt gccttctcat tgacagatat ttttgacaca attggtacct
    19201 tgatcggtac aggtgaaaaa gttggtattg tagcgacaaa tgatgaaaat caccaatcag
    19261 ccaaattgga taaggctctt tactctgatt tgattggaac gacagtcggt gccattgcag
    19321 gtacttcaaa cgtaacgact tatgttgagt ctgctgctgg tatcggtgca ggtggacgta
    19381 ctggtttgac agccttggtt gtagctatct gttttgcgat ttcaagcttc tttagcccac
    19441 ttctagcgat cgtaccaaca gcggctacag ctccaatctt gattatcgtt gggattatga
    19501 tgcttggtag cttgaaaaat atccattggg atgatatgtc tgaagcagtt cctgccttct
    19561 tcacatctat ctttatggga ttcagctact ctatcactca agggattgca gttggtttct
    19621 tgacttacac tttgactaag cttgtcaaag gtcaagttaa agatgttcat gccatgattt
    19681 ggattttgga tgccttgttt atccttaact acatcagcat ggccttataa tagaatgacc
    19741 cagggggatt tccccctttt tttaatacaa aggagatagg tgatgaaaga gaaaaatatg
    19801 tggaaagaat tgttgaatcg tgcaggctgg attttggtct ttttacttgc cgtcctttta
    19861 tatcaggttc ccctagtggt tacctctatt ttgactttaa aagaagtagc cctgctacag
    19921 tcagggctga tagttgctgg cctttcaatt gtggttctgg ctctatttat tatgggagct
    19981 cgtaaaacca agttagctag ttttaatttt tcttttttta gagctaaaga tttggcacgt
    20041 ttggtcttga gttatttagt cattatctgt tcaaatatac ttggttctat cttgttgcaa
    20101 ctgtcaaatg agacgacaac agctaaccag tctcagatta acgatatggt tcagaatagt
    20161 tcgttgattt ctagcttctt cttgctagcc ttgcttgctc caatttgtga ggaaatcttg
    20221 tgccgtggga ttgttcctaa aaagattttc cgaggcaagg agaacttggg atttgtagtc
    20281 ggtacgattg tgtttgcttt attgcatcaa ccaagtaatt taccttcttt attgatttat
    20341 ggaggtatgt cgacagttct atcttggacg gcctacaaga cccaacgttt ggaaatgtcg
    20401 atcttgcttc acatgattgt taatgggatt gctttctgtt tgttggctct tgtggtgatt
    20461 atgagtcgga cattaggaat ttctgtgtaa aagtttttat gtaggaac
//
LOCUS       DAVBGY010000040        20244 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVBGY010000040 DAVBGY010000000
VERSION     DAVBGY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20244
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.040"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            85..1065
                     /gene="folP"
                     /locus_tag="VQ620_001409"
     CDS             85..1065
                     /gene="folP"
                     /locus_tag="VQ620_001409"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET2764016.1"
                     /translation="MVKSLEGVTDMSSKANHAKTAICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEIEEEIQRVVPVIKAIRKESDVLISI
                     DTWKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSS
                     LIFPHFGFGQAFTEEELADFETLPIEDLMEAFFDRALSRANQAGIAQENILLDLGIGF
                     GLTKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFLNRDT
                     ASAHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            1067..2389
                     /locus_tag="VQ620_001410"
     CDS             1067..2389
                     /locus_tag="VQ620_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET2764017.1"
                     /translation="MKEFENNQWIAHYRTDQPHFGLERMVELLALRGNPHLKLKVIHV
                     GGTNGKGSTIAFLKNILEKLGLRVGVFSSPFLIHYTDQISINGKSIPEARLEALMADY
                     QSLLEGEAAANLQGTTEFEIITAIAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            2370..2924
                     /gene="folE"
                     /locus_tag="VQ620_001411"
     CDS             2370..2924
                     /gene="folE"
                     /locus_tag="VQ620_001411"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET2764018.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            2967..3779
                     /gene="folK"
                     /locus_tag="VQ620_001412"
     CDS             2967..3779
                     /gene="folK"
                     /locus_tag="VQ620_001412"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET2764019.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQVNLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(3929..4246)
                     /locus_tag="VQ620_001413"
     CDS             complement(3929..4246)
                     /locus_tag="VQ620_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HET2764020.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            4602..5048
                     /gene="rplM"
                     /locus_tag="VQ620_001414"
     CDS             4602..5048
                     /gene="rplM"
                     /locus_tag="VQ620_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET2764021.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            5067..5459
                     /gene="rpsI"
                     /locus_tag="VQ620_001415"
     CDS             5067..5459
                     /gene="rpsI"
                     /locus_tag="VQ620_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET2764022.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVIGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            5808..5960
                     /locus_tag="VQ620_001416"
                     /pseudo
     CDS             5808..5960
                     /locus_tag="VQ620_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6116..6454
                     /locus_tag="VQ620_001417"
     CDS             6116..6454
                     /locus_tag="VQ620_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET2764023.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQETLDKHHQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            6644..8080
                     /locus_tag="VQ620_001418"
     CDS             6644..8080
                     /locus_tag="VQ620_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET2764024.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVI
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGENREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTAQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            8132..8371
                     /locus_tag="VQ620_001419"
     CDS             8132..8371
                     /locus_tag="VQ620_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HET2764025.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WIAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            8501..8815
                     /locus_tag="VQ620_001420"
     CDS             8501..8815
                     /locus_tag="VQ620_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HET2764026.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            8933..10912
                     /locus_tag="VQ620_001421"
     CDS             8933..10912
                     /locus_tag="VQ620_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET2764027.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFAQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNSFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            10922..11236
                     /locus_tag="VQ620_001422"
     CDS             10922..11236
                     /locus_tag="VQ620_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HET2764028.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            11267..11773
                     /locus_tag="VQ620_001423"
     CDS             11267..11773
                     /locus_tag="VQ620_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764029.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFISPSPIIIFPIMLIVFSILATIEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFENLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            11853..13199
                     /gene="celB"
                     /locus_tag="VQ620_001424"
     CDS             11853..13199
                     /gene="celB"
                     /locus_tag="VQ620_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HET2764030.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNSLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            13579..13755
                     /locus_tag="VQ620_001425"
     CDS             13579..13755
                     /locus_tag="VQ620_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764031.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALSLGMT"
     gene            14157..16370
                     /locus_tag="VQ620_001426"
     CDS             14157..16370
                     /locus_tag="VQ620_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HET2764032.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEDEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(16592..17068)
                     /locus_tag="VQ620_001427"
     CDS             complement(16592..17068)
                     /locus_tag="VQ620_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET2764033.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRVEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            17291..20083
                     /locus_tag="VQ620_001428"
                     /pseudo
     CDS             17291..20083
                     /locus_tag="VQ620_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
BASE COUNT         6537 a         3199 c         4313 g         6195 t
ORIGIN      
        1 taggaaccga cctctttcta ctgggaaaaa gatgaatgca atcgtggcca tctttttctt
       61 tttatggtaa aatagagaaa taatatggtg aaaagccttg agggagtgac cgatatgtca
      121 agtaaagcaa atcatgcaaa aacagctatt tgcggaatta tcaatgtaac cccagactcc
      181 ttttcggatg gtggtcaatt ttttgctctt gagcaggcac tccagcaggc tcgtaaattg
      241 atagcagaag gggctagcat gctagatatc ggtggagaat cgactcggcc gggaagtagc
      301 tatgttgaga tagaaataga agaggaaatc cagcgtgttg ttccagtgat taaagcgatt
      361 cgcaaggaaa gtgatgtcct catctccatt gatacttgga agagtcaagt agcagaagct
      421 gctttggctg ctggtgccaa tctagtcaat gatatcacgg gtctcatggg tgatgaaaaa
      481 atggcccatg tggttgcaaa ggctggagcg aaagtagtca ttatgtttaa tccagtcatg
      541 gcgcgacctc agcatcctag ctcgctcata tttcctcatt ttggctttgg tcaagctttt
      601 acagaggaag agttagctga ctttgaaaca ttgccaatcg aagacttgat ggaggctttc
      661 tttgacagag ccctatcgag agcgaatcaa gctgggatcg cacaagaaaa tatcctgttg
      721 gatctaggga ttggatttgg tctgaccaag aaagaaaatt tgcttctttt acgggacctg
      781 gataaactac atcagaaggg ctatccaatc tttcttggag tgtcgcgcaa gcggtttgtc
      841 atcaatatcc ttgaagaaaa tggttttgaa gtcaatcctg agacagaact tggtttcctt
      901 aatcgggaca cggcctctgc tcatgtaacc agtatcgctg cgagacaggg ggtagaagtg
      961 gtacgagtgc atgatgtagc tagtcacagg atggcagttg aaattgcctc cgctattcgt
     1021 ctggctgatg aagcggaaaa tttagattta aaacaatata aataagatga aagaatttga
     1081 aaacaatcag tggattgctc actatcggac ggaccagccg cattttggat tggaacgaat
     1141 ggtggaactg ttagctttgc gtggcaatcc ccatctaaaa ctcaaggtca tccatgtcgg
     1201 agggaccaat ggtaagggtt cgaccattgc ttttttaaaa aatattctag aaaagctagg
     1261 actgagagtt ggcgtgttta gctcgccctt tctcattcat tatacagatc agattagcat
     1321 caatgggaaa tctatcccag aggctagact agaagccctc atggcagact atcagtcgtt
     1381 gctggaggga gaagcggccg ccaatttgca ggggacaact gagtttgaga taattacagc
     1441 catagcctat gactactttg cctcagagca agtagatgtg gccatcatgg aagtgggcat
     1501 gggcggactt ttagatagta ccaatgtttg ccagcccatt ttgacaggaa ttacaactat
     1561 tggcttggat catgtggccc tacttggtga caccttggag gccatagcag agcagaaggc
     1621 aggtattatc aaacaaggga tgcccttggt aacagggcgt attgctccag aagccttgac
     1681 tgtgattgac cgcattgcgg aagggaaaga tgcgccgaga cttgcctacg ggacagatta
     1741 tcaggttcgt catcaagaaa gtgtggtgac aggggaagtc tttgactata caagtgctgt
     1801 cagacaaggt cgcttccaga ctagcctgct tggtttgtac caaatagaga atgctgggat
     1861 ggccatagct ttacttgata ctttttgtca agaagatggt cgagagctag caagcaatga
     1921 ctttcttggt caagccttgg aagaaacaag ttggccaggg cgtttggaaa tcgtgtcaag
     1981 agatcccttg atgattttgg atggagccca taatccccat gctatcaagg ccttgttggt
     2041 aaccttgcaa gaacgttttg cggattatca taaggaaatc ctcttcactt gtatcaaaac
     2101 caaagccttg gaggatatgt tggacttgct gggagccatg ccagataccg agcttactct
     2161 aacacatttt gcggatagtc gggcgacgga tgaaagcgtg ctgaaagagg cagctaagtc
     2221 tagaaatctc agctaccaag attggcatga ttttctagag cagaatttga cagataaaaa
     2281 agaagagaaa caaacagtta ggattgtcac aggttccttg tatttcttga gccaagtgag
     2341 ggcctatctg atggagagga agaacgagaa tggatacaca aaagattgaa gcagctgtaa
     2401 aaatgattat cgaggctgta ggagaggacg ctaatcgcga gggcttgcag gaaacacctg
     2461 ctcgtgtagc ccgtatgtat caagagattt tttcaggtct tggtcaaaca gcagaggaac
     2521 atttgtcaaa atcctttgaa attattgacg ataatatggt ggtagaaaag gatatctttt
     2581 tccataccat gtgtgaacac cacttcttgc cattttatgg tagagcgcac attgcctaca
     2641 ttccagatgg tcgtgtggca ggcttgtcta agctagcccg tacggttgaa gtttattcga
     2701 aaaaaccaca aattcaagaa cgtttgaata tcgaagtggc cgatgccttg atggactatc
     2761 taggtgctaa aggagccttt gttgtcattg aggcggaaca tatgtgtatg agtatgcgtg
     2821 gtgttagaaa accaggcact gcaaccttga cgacagtagc tcgtggtcta tttgaaacag
     2881 ataaggacct ccgcgaccaa gcttatcgtt taatggggct ataaaaagaa tccgcttcaa
     2941 gcggattttt ctagaaagga atcattatgg atcaactgca gattaaggat ttggaaatgt
     3001 ttgcctatca tggtcttttt cctagtgaga aagaattggg gcagaaattt gtcgtttcag
     3061 ccatcctatc ctatgatatg accaaggcag ctacagactt ggatttaaca gcctctgtcc
     3121 attacggaga attgtgtcag cagtggacga cttggtttca ggaaacgagt gaagatttga
     3181 ttgaaacggt agcctataaa ctggtggaac gtacctttga gtcttatcct cttgtccaag
     3241 aaatgaagtt ggaactgaaa aaaccttggg caccggtgca tttgtcacta gatacttgct
     3301 cggtaaccat tcatcgccgc aagcaacgag cctttatcgc cctaggaagc aatatgggag
     3361 ataaacaagt aaacttgaag caagccattg acaaactgcg agctcgtggc atccatattc
     3421 tcaaagagtc cagtgtctta gcgacggagc cttggggtgg agtggagcag gatagctttg
     3481 ccaatcaagt ggttgaggtg gaaacctggc taccagcaca agacttgtta gaaaccttgt
     3541 tagccattga gtcagagctg ggacgggtga gagaagtgca ttggggacct cgtttgattg
     3601 atttggactt gctctttgtg gaggaccaga tcctttatac agacgacctc atattgcctc
     3661 atccttacat agcggaacgc ctttttgtcc ttgagtcctt acaggaaatt gcgcctcatt
     3721 ttatccatcc gatattaaaa caaccgatcc gcaacttgta tgatgctttg aaaaaataga
     3781 aaaactctag ttttcagtta cttgcaactg aaggctagag tttttatact cttcgaaaat
     3841 ctcttcaaac cacgtcagcg tcgccttacc gtactcaagt acagcttgcg gctagcttcc
     3901 tagtttgctc tttgattttc attgagtatt aaaataggtc attttcttct gggaggagga
     3961 tagtttctct accgtccatg tctaaaacca gtactcttgg gggataacga gggtcgaaag
     4021 gatggttaaa gtcaaaatca atggctgtag ggaggtgttg acttgaaaag tggaaggtaa
     4081 tctttccttg gttattaagc aattgaaact cgagttcttc ttccaattca aagacatttt
     4141 ttaagaaatg gtcgatgata taccaaaaag agtcaatgat gtcatcaggc aagctggtaa
     4201 caatgccaaa actagcagat cgcatgtggg tattggtaaa agccatatct ctgtcccctt
     4261 tcttttccct tatcatacag caaataggat taaaaatcaa gaaaaggtga ttttttgaaa
     4321 aggattttag ttacagggag aaatagggaa aaaattccta aaaatctacc gaagttaata
     4381 ggtaaattcc caaattaact tgattatata actttcagtt actttgagaa gttaccgaaa
     4441 aatatttttc atatctattg acttttaggg gtaaaatttg gtatgatagt aggcggtatt
     4501 gtttacccca tttgaaaggc cccggaacct tccaaatact tttcgatggg aaggaacacc
     4561 catcaccgta aacaaaaatc gaactatata taggagaaat catgaacaaa acaacattta
     4621 tggctaaacc aggccaagtt gaacgtaaat ggtacgtagt tgacgcaact gatgtaccac
     4681 ttggacgtct ttctgcagta gttgctagcg tacttcgcgg aaaaaacaaa ccaacattta
     4741 caccacacac tgatacaggt gacttcgtga ttgttatcaa tgctgaaaaa gttaaattga
     4801 ctggtaaaaa agcaactgat aaaatctact acactcactc aaaccaccca ggtggattga
     4861 aacaaatctc tgcaggtgaa cttcgttcta aaaatgcagt acgtttgatt gagaaatcag
     4921 ttaaaggtat gcttccacac aatacacttg gacgcgctca aggtatgaag ttgaaagtat
     4981 ttgttggagc tgagcacact cacgctgcac aacaaccaga agttcttgac atttcaggac
     5041 ttatctaagg aaaggaacaa taaagtatgt cacaagcaca atatgcaggt actggacgtc
     5101 gtaaaaacgc tgttgcacgc gttcgccttg ttccaggaac tggtaaaatc actgttaaca
     5161 aaaaagatgt tgaagagtac atcccacacg ctgaccttcg tcttgtcatc aaccaaccat
     5221 tcgcagttac ttcaactgta ggttcatacg acgttttcgt taacgttata ggtggtggat
     5281 acgctggtca atcaggagct atccgtcacg gtatcgctcg tgcccttctt caagtagacc
     5341 cagacttccg cgattcattg aaacgcgcag gacttcttac acgtgactca cgtaaagttg
     5401 aacgtaagaa accaggtctt aagaaagctc gtaaagcatc acaatttagt aaacgttaat
     5461 tcgaaagaat tactatactt atacagagca cctttcgggg tgttcttttt ttatactttc
     5521 ttactaaatt ggtgcaattg acacagttgt tgcgacttta gtcgcttaca aatgtgactg
     5581 caacctgaca tggtcagttg cctcaaaacg ttaatcaata cgattatatc aacgtttcaa
     5641 agcactcaaa agtttacctt atgggtgctt ttttcgtgtt tttttgaaaa gtttacctta
     5701 tttttatagt aaggtgaaat aagaatagga caaaccgagg atagtcaaat cgaattctaa
     5761 caatgtttta gaagtagagg tgtactattc tagtttcaat ctactatatt atggataatg
     5821 caagattcca tagaatgggg aagctagaac tcttgtgtga agagtttagg cgtaaacttt
     5881 tacctcttcc tccctactca cctgagtaca atcctattga gaaaacatgg gctcttttgt
     5941 cctgctcttg tttcaattga ctataaatcg ctgtaagaaa aaatcatttt attggtataa
     6001 agaagtgatt gaaagtaatg gcgaaacgtt agataattag ttcataaaaa acaagtttag
     6061 ctcctcatct aaacttgttt ttttaaaatt gaaggatgat tttggaggat ataagatgac
     6121 attgactatt catgtatatt ataaaggtga tggagatagt gcaataaagt ttgcaagaga
     6181 aatgcttgat agtggccttg ttgaagaaat tagaaatcaa aaaggtaact tgaaatacga
     6241 atatttttta ccaattgaaa aagagggaac tattctcctt attgatcagt ggataaatca
     6301 agaaacttta gataagcatc atcaatcaaa gacaatgcag aaaatcttag atttaagaaa
     6361 aaaatatcac ttgcagatgc aagttgaacg ttatatagaa gatgattctg ggatgccaga
     6421 aagcgataag tcgtttatag acacagggaa ttagaattat tgtgagagtt tttgtagttt
     6481 gaaagtattg aaaatcgtaa agatatcatt acgagaatac aagaagatat tgttctattt
     6541 ccattaaaaa ttacttcttt ccttaacaat aaggaaaaag aatgcgtttt catcttttgg
     6601 tttgaggttt ataataagag tatcaaagtc aggaggaaaa aagatgacta cttttaaaga
     6661 tggattttta tggggtggtg ctgttgctgc tcatcaactt gaaggtggat ggcaagaagg
     6721 tggcaaggga attagtgttg ctgatgttat gactgctggt cgtcatggag tagctcgtga
     6781 aattactttg ggagttttag agggtaaata ttatccaaat catgaggcga tagattttta
     6841 tcatcgttat aaagaagaca tagcactttt tgctgaaatg ggattcaagt gcttccgtac
     6901 ctctattgca tggacacgta tctttccaaa aggtgatgag ttagagccga atgaagaagg
     6961 attacagttt tatgataatc tttttgatga atgcttaaag aatggtattg aacctgttat
     7021 cactctatct cattttgaaa tgccttatca cttagtgacc gaatatggtg gttggaaaaa
     7081 taggaaattg attgatttct ttgctcgttt tgcagaagtc atatttaagc gttacaaaga
     7141 taaggttaaa tattggatga ctttcaatga aatcaataat caagcgaatt atcaggaaga
     7201 ttttgcacca tttactaact caggtattgt atatgaggaa ggtgagaata gagaggcaat
     7261 tatgtatcaa gcagcacatt acgaattagt tgcttctgca cgagctgtaa aaattggtca
     7321 tgagattaat ccagattttc aaataggttg tatgattgcg atgtgtccaa tttatccagc
     7381 tacttgcaat cctaaggata tcttaatggc aatgaaagct atgcaaaagc gttattattt
     7441 tgctgatgtg catgttttag gtaaatatcc tgagcatatt ttcaagtatt gggaacgaaa
     7501 aggtatttca gttgatttta ctgcccagga taaagaagat ttacttggtg ggactgtaga
     7561 ttacattggt ttcagttact atatgtcctt tgctatcgac tctcatcgtg aaaataatcc
     7621 ttattttgat tatcttgaaa cagaagattt agtgaaaaat aattatgtta aggcttctga
     7681 atgggagtgg caaattgatc cagaaggttt gcgttatgcg ttaaattggt ttacagacca
     7741 ctatcactta ccactcttta ttgttgaaaa tggttttgga gctatagatc aagttgcagc
     7801 agatggtatg gtacatgatg attatagaat tgaatatcta ggtgcccata ttcgtgaaat
     7861 gaaaaaggct gtagttgaag atggtgttga tttaatgggt tatactccat ggggatgtat
     7921 tgatttggtt tcagctggta ccggtgaaat gcggaaacgt tatggcttta tttatgtaga
     7981 taaagatgat aatgggaagg gaagttataa tcgttccccg aaaaaatctt ttggctggta
     8041 taaggaagtt atttcatcta acggtgaatc agtagaatag tataaacaaa ctgagattat
     8101 tctcagtttg tttagcatat tgaggtgaca tatgattgga agtattgcag caattttaac
     8161 aacatttgca tttttaccac aagtttttcg tgtagttaag acaaaggaca caggatctat
     8221 tgctctaggt atgtatgtta tgcaagttat tggtattgca ttgtggattg ctcatggtat
     8281 tcgtatagga gacttgcctt tgattttagc aaatagtgtt tcatttctac tctcaggaat
     8341 aattttattt tataaattga agtataaata atttttttcc atatcattta ggaaaatgag
     8401 ggaattgtca ttggaaacgc tttattttat aataaaaata gataacaaat aagccaactt
     8461 agcacagctt tgattggatt taagaaaagg agaaaatatt atggcaaaag ctttaattat
     8521 ttgtgcagca ggaatgtctt catcacttat ggcacagaaa acaacagagt ttctaaaagg
     8581 aaaagggaaa gatattgtag ttgatgctat tagtgctact gaaggaggga aagctatcgc
     8641 agcagcagct tttgatttgt atcttgtcag ccctcaaaca aaaatgtact ttaagcaatt
     8701 tgaagaggct ggttctaaag caggaaaacc agttgtacaa atacctcctc aagcctatat
     8761 tcctattcca atgggaattg aaaaaatggc taatttgatt cttgagaata tttgatgaac
     8821 acatgaaatg tcaataaata tttaagttaa gataaattgt tttgagtttg atataaattg
     8881 atttaagtgt ataatatgct tcaatatatg aaaacatacg ttttaggagg gaatgaatgt
     8941 gttaaacatt aaagagaaag atatccttca atttttaata aaaaataagg aacgttttgt
     9001 tacaagcaaa gaactggcag aatatttatc ctgttcagat agaactgtcc gtaatgtact
     9061 gaaattaatt gagaagacaa tgattattca aggagtccgg ttgatttcaa aacaaggtca
     9121 agggtatcaa atattttttg agaatcaagg cgcttaccag gagtttagac aaacttatga
     9181 actggaagaa gactatacga aaactgcggt ttcaaaaggt gatgatcgtt tagtatttat
     9241 cttaaataaa ttattatttg agcaagttcc agttcttttt gatgatttag ctgatgaact
     9301 ttacgttagt cgttcaacat tatctcatga tttcagaaag atacgagtga tgttatcaga
     9361 gtataatttg tccattgaga gtagggcgaa taaaggagtt tatgtctcag gcgaggaacg
     9421 tgataaacga cgttttatca taaattattt cctagagaat caatttttta aaacaataca
     9481 ttgctatgta aaatttaact tttttgctca aactgttcca ttagaagaat ttgctagaat
     9541 tgttttggat gaatgccaag aggccaacct aaaactatct gattttgtac tacagaattt
     9601 agtagttcac atagcattgt ctgtgattcg tttaaaatcc ggatttgaaa taaaaaatat
     9661 agactgtcag atgactgatg atgcaataga gagaaaggtt gctcaaagaa ttctttctaa
     9721 agtaagagaa gtaacaaatc aggaatttcc agttcaggaa atagactaca ttactttgca
     9781 tttgttagct aagagtcaac aatgtcaaaa aaatcagaag aatatttctg aagaagtttt
     9841 aaaaaaatct ttatttaaaa catttcagaa tttaggatta gatgatatgt ataacttttc
     9901 ttcagatttc caactaattg aaggattgat aactcatcta atgacactac aagtacgttt
     9961 agaaagccgg attaccttaa ataacccttt ggtagatgaa attaaacaga attatagtga
    10021 tatattcttt atgactagag aaattcttgc aaatatggac aagttcttgg agtggacaat
    10081 atcagatgat gaagtagcat atgtaagtct tcacttttta gctgctatgg aacgtagcaa
    10141 agagagtact aagtttaata ttcttgctat ttgtgcaaca ggatttggtg cagcccaaat
    10201 gttaagaaat cgtttggaaa cggagtttgg gaagcgagta gaagttgttg atgtaatagg
    10261 ctattatgaa ttgaatcaag aaaaacttaa aggaatagat tttatagtgt cagctgttga
    10321 tttatcaaat ctatattttc aaattccagt ttttaaagtt agtgtgtttc ttaagagtga
    10381 tgagatggaa atgattagaa aagctatgga tcagatgcaa gtttctagtt atgtacaatc
    10441 atcaaagatt aataaatttg aaaacaacag ctttagacag tattttagta aagaaaattt
    10501 tttaatatgt actgaatcag acaaggtaaa tttacttgaa aagatggttg agagtttatc
    10561 tgttggtgaa tcaaatgagt ttgaacaatc tttactttat ggaattaaac agcgcgaaga
    10621 attaagttca gttgtttttt ctgaaaaaat tgcagttccg catcctattc agccctttgg
    10681 tacagaggga aaggtttcgg ttgcaatttg taaagattcg ttactctggg ataatcaaag
    10741 ttcacatgtc cagctagtat ttcttctatc tccatcaata tatgggaacg aaggtttggc
    10801 aactgtgact aaaaagatag tctctctgac agagaatgat gaattgcaaa atcagctaat
    10861 atcttgcaat aactttgaag attttattaa catatttgag aagataaaat aggaggttca
    10921 tatgaatcaa gaggaactgc aggttgcagc atttgaaatt attctgcatt cgggaaatgc
    10981 aaggtcagaa atacatgaag catttgcaaa aatgagagaa ggtagttttg atgatgcaga
    11041 aagtaaatta aatcaatcaa atgagataat tcttgaagcg catcatgcac aaaccaaact
    11101 cttgcaagaa tacgcaagtg gagtggaaat taaaattgaa attatcatgg tacatgctca
    11161 agaccattta atgaccacta tgacattatt agaagtagca aaggaaatgt tagctcttta
    11221 taagaaagtt aactaaatat gtgttgttat ataaggagga aatgatatgg ataaaaaaga
    11281 acgacaaaaa attgaacaac aacgtcgaga gatggctcta actaatactt tttttaatcg
    11341 atatcttctt ctacgttatt caatagcact cttctttttt gggaatattt attggctttt
    11401 gaatcagttt attagtccct cgcctattat tattttcccc attatgctca tagtattttc
    11461 tattttagca actattgaac aatttaaact ttatggaaat aggaaagaaa agttgggaat
    11521 aacactaatg tttgttagaa tacaaatgct tatttcaata ggattacttg ttttaacttg
    11581 gacaagttgg tttgagaatc tttttccgat ttttgaaaat aaccaagtag cacgtttatt
    11641 tgtttttgta gttcttctat taggtttagt tctgagtttg ttagacatta gaagaattaa
    11701 aaaaatttat aaacgaacag ataaggctta tcaacaattt gtccaattgg aaaagaactc
    11761 acttagttta taatcctgaa ctagtataat ttgtatttga aactagtctt taaaaagtaa
    11821 aaattattat gttttattaa ggaggtctat ttatggatca acaaaacggg ttgtttggtt
    11881 ttcttgaaaa ccatgttatg ggaccaatgg gcaaacttgc tcagtttaaa gtagtgcgtg
    11941 ctatcacggc tgcaggtatg gctgctgtac catttactat tgtaggatca atgtttttgg
    12001 tattcagtat tttgccacaa gctttctcat tttggccaat tgtggcagat attttctctg
    12061 cttcatttga taaattcaca tcactttaca tggttgcaaa ctatgcgact atgggttctc
    12121 tatctcttta tttcgttcta tcacttgcat atgaattgac aaaaatttat gcagaggagg
    12181 aagaactcaa tatgaattct cttaatggtg ccttgctcgc cttgatggct tttgtcatga
    12241 cagtaccgca aatcattttt gatggtggaa tgatgaagac tgtgacaagt ctaaaagaag
    12301 gtgcagtaat tgcagatgga tgggcaatgg gaaatggagt cgcacgtttt ggtacaacag
    12361 gaatttttac cgcaatcatt atggcaattg tgactgttct tatttatcgt atgtgtgtta
    12421 aacataattg ggttattaaa atgcctgaag ctgttccaga aggagtttct cgtggattta
    12481 ccgctttggt tccgggattt gttgttgcat ttgttgttat ctttatcaac ggtcttcttg
    12541 tagcaatggg aacagatatt tttaaagtca ttgcaattcc atttggtttt gtatccaatc
    12601 tgactaattc gtggattggt ttaatgatta tttatctatt gactcaacta ctttggattg
    12661 taggtatcca cggtgcgaac attgtttttg catttgttag tccaattgct cttgctaaca
    12721 tggctgaaaa tgctgctggc gggcacttcg ctgttgcagg tgaattttct aatatgtttg
    12781 taattgcagg tggttctggt gcaactttag gactatgttt atatattgct tttgcctcta
    12841 aatctgaaca gcttaaagca ataggacgag catctgtagt tccagcctta tttaatatta
    12901 atgaaccatt aatttttgga ttacctatta tctataatcc agccttggct ataccattta
    12961 ttttagcacc aatggttact gctactattt attacgtagc gaattctcta aactttatta
    13021 agccaattat cgcacaggtt ccatggccaa ctccagtagg gattggagct ttcttaggga
    13081 cagcagatct tcgagctgta ttagttgctc tagtatgtgc atttgcagca ttcctagtct
    13141 atcttccatt catccgtgta tatgatcaaa aattggtgaa agaagagcaa ggtatctaat
    13201 aagtaaaaaa gtatactcga tgatagaatc catatagttt aaataatgag cgttaacatt
    13261 agcagtgaag aggtcttttt aagatattct tctctgcttt ttcatatttt ttcgctatgt
    13321 agagtatgaa gcaaaataga aagtagttcg aatagttgat aaacagggaa ttgctgttat
    13381 ttaaaaaaaa caataaagta aagtatgtta tcgtagaata cgatacaata gtaaaaaagc
    13441 aaaaagaatg cgttttatat ttgggtttga ggcttataaa ataggataag cgtactgaaa
    13501 acttagatat gacaaacaaa ataaaatttt taaaagctac agagaaacat ggtataatgt
    13561 atctaaagga ggtagcatat gtccttagat atagataaag aaaagatgac aatcatggga
    13621 atagcttttg aaaaccgatc cgtttttaag agtgtttggt atgctttaag tacaaatatg
    13681 attgaaggat ggcgacctac ggttagtgat gttgaaaaat tacgagatga agctctttct
    13741 ctggggatga cttaatgaag cgcaactgtt atgagtctta tgactatgtt gatccggata
    13801 atttatatac cgatccgggt tcttcagttc taagaaataa gcaggaagga aaaaagcacg
    13861 cgagcttgaa tatcgtatga ttgctagtaa gagtttgaaa ttatttatca atcctatctt
    13921 agttaatagt ttaaaagtag tttgtttcac aactgcataa gcttagaaaa gttgaaaagt
    13981 gataactctt taataaactc ttggtttgtt aaaagatatt ttgtccacta catttttttc
    14041 aattgactat tatttgtcat agatataaca gatagaacat tgcattcaga ataatgaaaa
    14101 acgcatcaaa aatggtgcgt tctgttttta tgctaccata aaaggaggaa aacaaattga
    14161 aaatttttaa gggagagttt tatcgaatct ctgtattaac agacaagcta gtaaggttag
    14221 aatactctcg aactggaaat tttgaggata gaacgacaca acttatctat aatagagatt
    14281 ttggccaagt ttcgttagat tatatcgaga catcaaatgt actagatatt atgacggact
    14341 attttcatct gcactttaat aaaggagaat ttaacgccga aaatttattt atagaattaa
    14401 aaggaaattt tgccgtatat ggtagtcgct ggtattttgg tgaatctatt gaaacgttaa
    14461 aaggaacagc tcggactctg gataaggcag atggagcaat ctcgttagaa gatggaatta
    14521 ttagccgaaa tggtatagcc ttattggatg attctcaagg atttatttgg gatgaacaat
    14581 ctggttatat tgagagagaa aatcaaattg acctgtattt ctttgcctat gggcatgatt
    14641 atagaggagc aatcagagac ttttaccatt tgactggttc aacacccttg ttgccaagat
    14701 atgctttagg caattggtgg agtagatatt ggccttatac gtcggatgaa tacttggatt
    14761 taatagacag atttgaaaca gagaaaattc cattatctat cggtgtgtta gatatggatt
    14821 ggcatataac tgacattcca gctcgttttg gaagtggctg gacaggatat agttggaata
    14881 gaaacttaat accaaatcca gaacagttat tgcaacaact tcatgataga aagttaaaac
    14941 tctccttaaa tgtccatcct gctgatggga tacgggctta tgaagaagct tatcctcaaa
    15001 tcgcaaaacg gttggggtta aatgtagaac tagaagaacc tgctattttt gattttttta
    15061 atccctcttt tagggaagcc tactttaaag atgttcatta tgagctagaa aagcagggag
    15121 tagatttttg gtggattgac tggcaacaag ggacacaagg tatgctagat ccactttggc
    15181 ttttaaacca ttatcactat caggatagtt gtaaaaatgc agaaggtggt ttgattttat
    15241 caagatatgc aggtcctggt agtcaccgct accctgttgg tttttcaggg gacactatta
    15301 ttagttggaa ttccttaaga tttcaaccct attttacagc gacagcatct aatatcggtt
    15361 atagttggtg gagtcatgat atcggtggac atatgttggg ggattatgac gaagagctac
    15421 aaactagatg gctacagttt ggtgttttta gtccgattac tcgattacat agttctagaa
    15481 gtccttttaa tagtaaagaa ccttggttct tttcagaaac aacatctaag attatgaaga
    15541 aataccttcg tttgagacat cagatgattc cctatctata taccatgaat gtaaagacac
    15601 atgaggaagg tgccccatta atcagtccaa tctattattt ctatccagag aatgatgaga
    15661 gctataatgt tccaaaccaa tacttttttg gaacagaact gatggtggct cccattgtag
    15721 aaaagatgga tttgacattc caatctgcaa aagtagatgt atggttccct gaagatgaat
    15781 ggtatgactt cttttcagag aaaaaataca caggtggtgt gaagttaagt gtttataggg
    15841 acatctcgac tacgcctgtg tttgcaaaaa gtggtgcaat cattcccttg gttggttctg
    15901 agataggtat gggtgttgat ttacctgagg ttgtagattg gtatgtattc ccagggaaac
    15961 aacattcttt tgaaatgctt gaagatcaaa atggtcaaag atataaaaca agattatcaa
    16021 tcgattggga aatgggaatg gtagagttag cattacaagg agattctagt atcgttccaa
    16081 gcaatagaaa acatagaatt cattttaaag gaacgaatgt gtctataatt gaattgccaa
    16141 ataagaatga tacagctaaa tttgaatgga aagataataa aaggacatct ttaaatgacg
    16201 aagtttttag actactaaag acagcttctc ttccatatga attaaaagat agattgttaa
    16261 atcaattcat caatgcgaaa aactctcatg acttaatgaa tatcttgcat catcaggata
    16321 aggaattgag agggcgtttg ttggaaatga tatttactag ccaaaactaa ttgaaataat
    16381 tagaaagtca tttgtttagt gtatatgaag attctgtatg gtattcgtta tcactggtca
    16441 cattgaaatt gtgaaataag cacctacaac atagacttta caacacttca tgtttcatat
    16501 catggggtgt ttttctattt aagtcaaagc caatcaaaaa atctcactat tcatcaggtt
    16561 aaaggaaacc taatcatagt gagattgtga attatagtag agtttgtatc gcctcttcaa
    16621 tttgttttgg gtctgttttt gaagaaaagc gttcaaagac ttgcccatct cgaccgatga
    16681 gaaacttagc gaaattccat tcgactcgtt ttcctagtgg gccggatttc tggtctttta
    16741 accagacata gagagggtct gcttccttac cgttgacctt aatcttggca aaacgtggga
    16801 aggtggtttg aaaatgtagg ctacagaagg cgttgatttc ctctgcgctg ccgggtgctt
    16861 gtcccataaa ctgattgcaa gggaaatcca atatttcgaa gccctgatct tgatagcgtt
    16921 catagagttc ttgaagtccc tggtactggg gcgttaaacc acatccagta gcagtgttga
    16981 caatcaagag aactttgcca cgatagctat ccaagggagt tgcttggttg ttttggttca
    17041 aaacggaaaa atcataaagt gaagtcatga gggcctcttt tctttttcta cattgtaaca
    17101 gtttttactt ttttcgtcga gtcggatgga agatattgtt gaagaataag ccttcaatat
    17161 gatagaaacc gtttacaaat aactttaaaa atggtatgat ttaattaaac cggtttactt
    17221 agttgctata aatctctaat ttgagattta gttcctattg attttacaat atgtttattg
    17281 gagtgtatac atgcaaacaa aaacaaagaa gctcattgtg agtttgtctt cacttgtttt
    17341 atcaggattt ttattaaacc attatatgac agttggagcg gaagaaacga ctacgaatac
    17401 cattcagcaa agccagaagg aagttcagta tcagcaaagg gatacaaaaa atttagttga
    17461 aaatggtgat tttggtcaga cggaggacgg aagcagtccg tggacaggaa gcaaagctca
    17521 ggggtggtca gcttgggtag accagaagaa tagtgcagat gcctcaactc gagtcattga
    17581 ggctaaggat ggggctatca ctatctcaag ccctgagaaa ttaagggcag cgcttcaccg
    17641 tatggttcct attgaagcta agaaaaagta taaactgcgt ttcaagatta aaacagataa
    17701 taaagtcggg attgctaaag ttcgtatcat tgaggaaagt ggtaaggaca agcgattgtg
    17761 gaattctgca acgacgtcag gaacaaagga ctggcagacc attgaagcag actatagccc
    17821 gactttagat gttgataaaa tcaagctgga gttattctat gaaacaggaa ctgggactgt
    17881 ttcctttaag gatattgagc tggtagaggt agcagaccag ctttctgagg attctcaaac
    17941 agataaacag cttgaggaaa agattgattt accaattgga aaaaaacatg ttttttctct
    18001 tgcggactat acttataagg tagaaaatcc tgacgttgct tcagtcaaaa atggaatttt
    18061 agaacctctt aaggaaggga caaccaatgt cattgtcagt aaagatggca aggaagtgaa
    18121 aaagattcct ttgaagattc tagcctctgc taaggatgca tacacagacc gtttggatga
    18181 ctggaatggc atcatcgctg ggaatcaata ctatgattct aaaaatgaac agatggccaa
    18241 attaaaccag gaattggaag gaaaggtagc tgatagccta tccagtattt caagtcaggc
    18301 ggaccgcatc tatttgtggg aaaaattttc aaattataaa acgtctgcaa atctgactgc
    18361 cacttatcgg aaattggagg agatggccaa gcaagtgacc aatccttctt ctcgttatta
    18421 taaagatgaa actgtcgttc gaacagtcag ggattccatg gaatggatgc ataaacatgt
    18481 ctacaatagt gaaaagagca ttgttgggaa ctggtgggat tatgaaatcg gtacacctcg
    18541 tgccatcaac aataccttgt ctctgatgaa agaatacttc tctgatgagg aaattaaaaa
    18601 atatacagat gtgattgaaa aatttgtacc agatcccgaa catttccgaa agacgactga
    18661 taacccattc aaggctctag gtggaaactt agttgatatg ggaagggtaa aagtaatagc
    18721 tggtttactg cgtaaggatg atcaagaaat ttcttctacc attcgctcga ttgagcaagt
    18781 gttcaagttg gtagaccaag gtgaaggttt ttatcaagat ggatcctata tcgaccacac
    18841 caatgttgcc tatacgggtg cttatgggaa tgttttgatt gatggcctgt ctcaactgtt
    18901 gccagtcatt caaaagacca agaatccaat cgataaagat aaaatgcaaa ccatgtacca
    18961 ctggattgat aaatcgtttg ctcctttgct ggtgaatgga gagctgatgg atatgagtcg
    19021 tggacgctcg atcagtcgtg caaatagcga tggctactgg ccaacagtta atccatataa
    19081 gatgcctggt acaacagaga cggatgctaa gagagcggat agcgatacag gtaaagtttt
    19141 accgtctgct ttcgttggaa cgagcaaact agatgatgcc aatgcgacag caaccatgga
    19201 tttcaccaac tggaatcaaa cattgactgc tcataagagc tggtttatgc taaaggataa
    19261 gattgccttt ttaggaagca atatccaaaa cacttcaaca gatactgctg caactacaat
    19321 cgaccagaga aaactggaat caagtaatcc atataaagtc tatgtcaatg ataaagaagc
    19381 ctcccttaca gaacaagaaa aggattatcc tgaaacccaa agtgtctttt tagaatcgtc
    19441 cgattcgaaa aagaatattg gttacttttt ctttaagaag agttcaatca gtatgagtaa
    19501 ggctttgcaa aagggagcct ggaagggtat caatgaagga cagtcagaca aggaagttga
    19561 aaatgaattt cttacgatta gtcaggctca taagtaaaat ggagattctt atggctatat
    19621 gctcattcct aacgtggatc gtgccacctt caatcaaatg ataaaagagt tagaaagtag
    19681 cctcatcgaa aataacgaaa cccttcagtc tgtttatgat gctaaacaag gagtttgggg
    19741 cattgtgaaa tatgatgatt ctgtctctac tatttccaac caattccaag ttttgaaacg
    19801 tggagtctat actattcgaa aagaagggga tgaatataag attgcctact ataatcctga
    19861 aactcaggaa tcagctccag atcaggaagt ctttaaaaag ctagagcaag cagctcagcc
    19921 acaagtacag aattcaaaag aaaaggaaaa atctgaagag gaaaagaacc attcggatca
    19981 aaagaatctc cctcagacag gagaaggtca gtcaatcttg gcaagtctag ggttcttgct
    20041 acttggggcg ttttatttat tccgtagagg aaagaacaac taatttgttc atcatctaga
    20101 tgaattacat gaaactgtgg aaaatagaaa aaatccgaag aaggctaaat atcttcggat
    20161 ttttctttta aaaagggata agttgatact tatccttttt tacttcatta tgtgactata
    20221 gcgttttgaa actagaatag gacg
//
LOCUS       DAVBGY010000041        20225 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVBGY010000041 DAVBGY010000000
VERSION     DAVBGY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20225
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.041"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            63..527
                     /locus_tag="VQ620_001429"
     CDS             63..527
                     /locus_tag="VQ620_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HET2764034.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            685..1110
                     /gene="lacA"
                     /locus_tag="VQ620_001430"
     CDS             685..1110
                     /gene="lacA"
                     /locus_tag="VQ620_001430"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HET2764035.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            1141..1656
                     /gene="lacB"
                     /locus_tag="VQ620_001431"
     CDS             1141..1656
                     /gene="lacB"
                     /locus_tag="VQ620_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HET2764036.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            1667..2596
                     /locus_tag="VQ620_001432"
     CDS             1667..2596
                     /locus_tag="VQ620_001432"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HET2764037.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDDQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKLVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLEELKEVLQESLFTGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAGLLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            2598..3578
                     /gene="lacD"
                     /locus_tag="VQ620_001433"
     CDS             2598..3578
                     /gene="lacD"
                     /locus_tag="VQ620_001433"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HET2764038.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKILVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3812..4648
                     /locus_tag="VQ620_001434"
     CDS             3812..4648
                     /locus_tag="VQ620_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2764039.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4684..5001
                     /locus_tag="VQ620_001435"
     CDS             4684..5001
                     /locus_tag="VQ620_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2764040.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            5003..6697
                     /locus_tag="VQ620_001436"
     CDS             5003..6697
                     /locus_tag="VQ620_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2764041.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERSGKSNDELKEKVAANFNTAKADAILEKA
                     GVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            6906..8312
                     /gene="lacG"
                     /locus_tag="VQ620_001437"
     CDS             6906..8312
                     /gene="lacG"
                     /locus_tag="VQ620_001437"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET2764042.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPLDPKNPSDVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            8355..8537
                     /locus_tag="VQ620_001438"
     CDS             8355..8537
                     /locus_tag="VQ620_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HET2764043.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLFLNFINPEE
                     WDTISFDSDFMPFQQS"
     gene            complement(8815..9576)
                     /locus_tag="VQ620_001439"
     CDS             complement(8815..9576)
                     /locus_tag="VQ620_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2764044.1"
                     /translation="MLKQEKLAKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(9758..10720)
                     /gene="nrdF"
                     /locus_tag="VQ620_001440"
     CDS             complement(9758..10720)
                     /gene="nrdF"
                     /locus_tag="VQ620_001440"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HET2764045.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(10908..13067)
                     /gene="nrdE"
                     /locus_tag="VQ620_001441"
     CDS             complement(10908..13067)
                     /gene="nrdE"
                     /locus_tag="VQ620_001441"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HET2764046.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(13148..13366)
                     /gene="nrdH"
                     /locus_tag="VQ620_001442"
     CDS             complement(13148..13366)
                     /gene="nrdH"
                     /locus_tag="VQ620_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HET2764047.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            13717..13980
                     /locus_tag="VQ620_001443"
     CDS             13717..13980
                     /locus_tag="VQ620_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HET2764048.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            13986..15719
                     /gene="ptsP"
                     /locus_tag="VQ620_001444"
     CDS             13986..15719
                     /gene="ptsP"
                     /locus_tag="VQ620_001444"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET2764049.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            15974..18424
                     /gene="phtA"
                     /locus_tag="VQ620_001445"
     CDS             15974..18424
                     /gene="phtA"
                     /locus_tag="VQ620_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HET2764050.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
     gene            18582..>20225
                     /locus_tag="VQ620_001446"
     CDS             18582..>20225
                     /locus_tag="VQ620_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2764051.1"
                     /translation="MKINKKYLAGSVATLVLSVCAYELGLHQAQTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSTNDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWLPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGNHYHF
                     IPYEQMSELEKRIARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPS
                     NPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLG
                     AKKTDLPSSDREFYNKTYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKL
                     VDDILAFLAPIRHPERLGKPNAQITYTDDEIQVAKLAGKY"
BASE COUNT         6465 a         3627 c         4171 g         5962 t
ORIGIN      
        1 aactagaatt ctagttttgc aggaagtcta ataaagaaac tagcaaggtc aggccttact
       61 ttattggtgg acatccagga tttaattgtc ttctacttga tgatgaagtc tatgaagatt
      121 attatctaga gtttgagaaa gaagagattt gctctgttcc acgtcctttc ccagaaacag
      181 gtatgttgga tttccaagat agaagtccat ggctagaggg acaaaaagaa atagatctta
      241 gttatgatct tttcagcaca gatgcagtga ctttggatga attgcaatct cgaacaattg
      301 cccttcgttc tcttaaacat gataagggat tgaaagtgca ttttgcagaa tttccaaacc
      361 tcatcatctg gtcaactttg aacaagggac ctttcattac ctttgaacca tggtctggct
      421 tgtcaacatt ccttgaagaa ggagatcatt tagaagataa gaagaatgtt tgtctcttag
      481 aagccaatca ggttgaagaa ttagggtttg agatagaagt tttataaaaa aagtaaaaaa
      541 taaataacaa aaacaaacac aaactgttga cttcttcaaa caatagtagt atattatgtt
      601 tgtgaggact aattgactgt tttttaaaga cagccaatac actgctggaa tccagcatag
      661 aaaaaataaa aaggagtata caatatgtct attgttatcg gtgcagatgc tgcaggtttg
      721 agattgaaag aagttgtgaa agacttctta gaaaaagaaa acttccacct tgtggatgtt
      781 acagctgaag gtcaagactt tgttgatgtg actctcgctg ttgctgcaga agtaaacaaa
      841 gaagaacaaa accttggtat cgtgattgat gcttatggag ctggtccatt tatggttgca
      901 actaagatca aaggaatggt tgctgcagaa gtatctgacg aacgttcagc ttatatgact
      961 cgtggccaca acaactcacg tatgatcact atgggggcac aacttgttgg tgatgaattg
     1021 gctaaaaaca tcgctaaagg atttgttaat ggtaaatacg acggtggacg tcatcaaatc
     1081 cgggttgaca tgttgaacaa aatgggctaa ttagattaca gtaagaaagg taagttaaaa
     1141 atgagaattg caattggatg cgaccacatc gtaacagatg aaaaaatggc ggtttcagaa
     1201 tttttgaaat caaaaggata tgaagtcatt gactttggta catatgacca tacacggact
     1261 cactacccaa tctttggtaa aaaagtaggg gaagctgtaa ctagcggcca agctgatctt
     1321 ggggtatgta tctgtggtac tggtgttggt atcaacaacg ctgtaaataa agttccaggt
     1381 gttcgttctg ccttggttcg tgatatgaca acagcccttt atgctaaaga acaattgaac
     1441 gctaacgtta tcggctttgg tggtaagatt actggtgaat tgcttatgtg tgatatcatc
     1501 gaagctttca ttcatgctga atacaaacca tctgaagaaa acaagaaatt gattgcgaaa
     1561 attgaacacc ttgaaagtca caatgctcaa caaacagacg caaacttctt tacagaattc
     1621 cttgaaaaat gggatcgcgg agaataccac gactaagagg tgacctatga ttttaacagt
     1681 cacaatgaac ccatccattg atatttccta tcccttggat gagttgaaga ttgatactgt
     1741 caatcgtgtg gtggatgtaa ccaaaacggc tggtggtaag ggacttaatg ttacccgagt
     1801 actttcagaa tttggtgatt ctgttcttgc tactggttta gtgggtggca aacttggtga
     1861 gtttttggtt gaacatatcg atgatcaagt aaagaaagat ttcttttcta ttcagggaga
     1921 aactcgtaac tgtatcgcta ttctccacgg tgacaaccaa acagaagttc ttgaaaaagg
     1981 tcctgaagta ttggaacagg aaggtcaaga ctttttggaa catttcaaaa aactcttgga
     2041 gtcagttgaa gtagtagcca tctcaggtag tctgccagct ggccttccag ttgattacta
     2101 tgcgagcttg gtagagcttg ctaatcaagc tggcaagcta gtagtcttgg actgctctgg
     2161 tgcagcactt caggcggttc ttgaatcacc ccataaaccg acagtaatca aacccaataa
     2221 tgaggaattg tctcagcttc taggaagaga agtttctgag gatttggagg aattaaaaga
     2281 agtccttcaa gaatctttgt ttacagggat tgaatggatt atcgtttcac ttggtgccaa
     2341 cggtactttt gccaaacatg gtgacacttt ctacaaggta gatattccta gaattcaggt
     2401 ggtaaatcct gttggatctg gagactctac tgtggcagga atttcttcag gacttcttca
     2461 caaagaatcg gatgcaggat tactcatcaa ggcaaatgtc cttggtatgc tcaatgctca
     2521 agaaaaaatg actggtcatg tcaacatggc caactatcaa gctctatatg atcaattaat
     2581 agtaaaagag gtataaaatg gctttaacag aacaaaaacg tgtacgctta gaaaaacttt
     2641 ctgatgaaaa tggtatcatc tcagctcttg catttgacca acgtggtgct ttgaaacgcc
     2701 tcatggctca acaccaaaca gaagaaccaa ctgtggccca aatggaagaa cttaaaatct
     2761 tggtagcaga tgaattgact aaatacgctt catctatgct tcttgaccct gagtatggac
     2821 ttccagcaac taaggctctt gatgaaaaag ctggtcttct ccttgcttat gaaaaaacag
     2881 gttatgacac aacaagcaca aaacgcttgc cagactgctt ggatgtttgg tctgcaaaac
     2941 gtattaaaga agaaggtgca gatgcagtta aattcttgct ttactatgat gtagatagct
     3001 cagacgaact caatcaagaa aaacaagcct acatcgaacg catcggttct gagtgtgtgg
     3061 ctgaagatat cccattcttc cttgaaatcc ttgcttacga tgaaaaaatt gcggacgcag
     3121 gttctgtaga atacgctaaa gtaaaaccac acaaagttat cggtgctatg aaagtctttt
     3181 cagacccacg ttttaacatt gatgtcttga aagttgaagt tcctgttaac attaaatatg
     3241 ttgaaggctt cgctgagggt gaagtggttt acacacgtga agaagcagca gccttcttca
     3301 aagcgcaaga tgaagcaacg aacttgccat acatctactt gagtgctggt gtatcagcta
     3361 aactcttcca agatactctt gtatttgctc atgaatcagg tgcaaacttt aacggagttc
     3421 tttgtggtcg tgctacatgg gcagggtcag ttgaagctta catcaaagat ggtgaagcag
     3481 cagctcgcga atggcttcgc acaactggat ttgaaaacat tgatgagctc aataaagttc
     3541 ttcaaacaac agcgacttca tggaaagaac gtgtgtaaga aagtcctcct agtttaggaa
     3601 catgaatcta aaaaaattta aaaaaagttg tatgtaaagg tttacaaaat aacttacttg
     3661 tgctatactt aaatcacaag ttaatacaag gtgagtgtta ctaagtaata ttaggcatga
     3721 tcacaggtga attagaaatc agctgatttt ctagttcatt tgtggtcatt ttttgtactc
     3781 atataccttt aagatataaa aggaggttga catgtatcga attctaaatc caatgaatca
     3841 caatgtctcg cttgtcagaa atgataaggg agaagaggtg attgtaattg gtaaggggat
     3901 tgcattcgga aagaagaagg gggatttgat tgctgaaaat catgttgaga aaatctttcg
     3961 gatgaagacc gaagagtcca gagaaaactt tatggctctt ctcaaagatg ttccgcttga
     4021 ttttatcaca gtgacctatg aaatcattga taagctatca aagaaatatc attatccgat
     4081 tcaagagtat ctctatgtaa ccttgacaga tcatatttac tgttcttatc aagctctaac
     4141 tcaaggaagg tacaaggata gtaatctgcc agatatttct gctaagtatc ctgtcgcttt
     4201 tcaaatcgca aatgaagctt ttgaaattta ccgtcagaag ctagcagatc attttcctga
     4261 ggacgaaatt attcggattg cttatcattt cattaatgct gaaggtgaaa atgaagtgga
     4321 acttgtggag tcgattgata agaggaaaga aattctcagg aatgttgaag aagttttaac
     4381 ggactatgca attcaacgaa ctaaaaagaa taaccatttc tatgatcgct ttatgatcca
     4441 tttgaattat ttcttggatt atttagacag atctagagat gataaccaat cacttctgga
     4501 tatggaagat catattaaac aatcctatcc aaaagccttc gagattggtt ccaagatcta
     4561 tgatgtgatt acgcaacata cgggtcttga tttgtataaa agtgaacgag tttatctagt
     4621 tctacatatc caacgtttat tgtcataaaa atttatttaa aactatataa ggagaattct
     4681 atcatgaata gagaagaagt aacattgtta ggttttgaaa tcgtagccta tgctggcgat
     4741 gctcgttcaa aactattgga agccttgaag gctgctgaag ctggtgattt tgaaaaagcg
     4801 gacgctctgg tagaggaagc tggtagctgt attgcagagg ctcaccacgc gcaaacaagt
     4861 ctattgacta aggaagcttc tggtgaggac ttagcgtata gtgtgaccat gatgcatggt
     4921 caagatcact tgatgacaac tatcttgtta aaagacctaa tgcaccattt aattgaactt
     4981 tacaagagag gagttaaata aaatggataa gttaataatt tttattgaga aaggaaagcc
     5041 tttctttgag aaattatcga gaaatatata cttaagagcg ataaaagatg gttttatttc
     5101 cagtatgcca gcagtattat tctcaagtat ttttatacta atagctgctg tgcccaatat
     5161 ttttgggttt aaatggtctg atgaacagtt agcttttatt ttgaaacctt acaactactc
     5221 aatggggatt ttagctctct tagtagctgg gacaactgca aaatcgttaa cagattcagt
     5281 taatacacgt agtatggaga aaaccaacca aatcaactac atgtcaactt ttttagcagc
     5341 tgttgttgga ttgttaatac tggcagctga tccaattgaa ggtggatttg ctaatggtct
     5401 tttaggtact agaggattac ttactgcatt tttggcagct tttattacag taaatatcta
     5461 taaagtatgt attaaaaata atgtaaccat tagacttcct gaagaggttc caccaaatat
     5521 tgcacaagtt tttaaagatg ttattccgtt tgccttatct gttctttcaa tatatggatt
     5581 ggatttaatt gttagaaata tttttggtac aaatgtagca gaatctgttg gtaaaatatt
     5641 agcccctcta ttttcagcta ctgatggtta tatcggttta gctattgtat ttggtgctta
     5701 tgctttcttt tggtttgttg gaattcatgg gccatctgtt gtagaacctt taattgtagc
     5761 tatcagttat gcaaatatag aagctaatgt tcagcttgtg caagctggta tgcacgcaga
     5821 taaaatttta aatccagtta ctcaaacttt tgttgtaaca atgggtggta ctggtgcaac
     5881 actagtagtt ccatttatgt ttatgtggtt atgtaaatca aaaagaaata gaattgtcgg
     5941 acgtgcgtct gtagttccta ctttctttgg cgtaaatgaa ccaatcctat ttggtgctcc
     6001 aattgtttta aatcctatct tctttattcc gtttgttact gctcctatta tcaatgtttg
     6061 gattatgaaa ttttttgtag atgttctaca aatgaatagt ttcagtatta ttttaccttg
     6121 gacaacacct gctccaattg gtattgttat gggaacggcg ctggctccat tgtcattcgt
     6181 cttggctata actttaatca ttatcgacac tttaatctat tatccatttg ttaaagttta
     6241 tgaccatcaa attttagaag aagaacgttc aggtaagtct aatgatgaat tgaaagaaaa
     6301 agttgctgca aacttcaaca ctgcaaaagc ggatgctatt cttgaaaaag cgggtgtcga
     6361 tgcagcacaa aataccatca ctgaagaaac aaatgtcctc gttctctgtg caggtggagg
     6421 aacaagtggt ctccttgcaa atgctttgaa taaggcagca gcagaataca atgtccctgt
     6481 gaaagcagca gcaggcggct atggtgctca ccgtgaaatg ttaccagagt ttgatcttgt
     6541 tatccttgcc cctcaagttg cttcaaactt tgaagatatg aaagcagaaa cagataagct
     6601 cggtattaaa ctagcgaaaa cagaaggcgc tcaatacatc aaattaactc gtgatggaaa
     6661 aggtgctctt gcattcgtac aagcgcaatt cgattaaggc tagagactct gaaatagtct
     6721 cctatcgtta cggaaatcgc tatggcgaat ttcctattat taattcgtcg gtaaaaagat
     6781 atcgttttta cctcctcatg tcacaattcg gtgacttggt acaagaagtg agatggagaa
     6841 ggatggctca ctgactcctc tcctctcact tttactttat ttaaatcaag aaataggtga
     6901 aaaaaatgac aaaaacactt ccaaaagact ttatttttgg tggcgcaaca gctgcctatc
     6961 aagcagaagg tgctacacat actgatggta aaggaccagt agcgtgggat aaatatcttg
     7021 aagataacta ctggtacact gcagaaccag ccagtgattt ttacaatcga tatccagttg
     7081 acctcaagct agcagaagag tatggtgtca atggtattcg aatttctatt gcttggtcac
     7141 gtattttccc gactggttac ggccaagtaa atgctaaagg tgttgagttt tatcataatt
     7201 tatttgcaga gtgtcacaaa cgtcatgttg agccctttgt aactcttcat cactttgaca
     7261 cgccagaagc tctccactca aatggagact tcttaaaccg tgaaaatatc gaacattttg
     7321 tagactacgc tgccttctgt tttgaagaat ttccagaagt aaactattgg acaaccttta
     7381 atgaaattgg gccaattggt gatggtcaat acttggttgg gaaattccct ccaggtattc
     7441 agtacgacct tgccaaagtc ttccaatcgc accacaatat gatggtgtct catgcgcgtg
     7501 cagttaagct atataaagat aaaggctata aaggcgaaat tggtgttgtt cacgctctgc
     7561 caactaagta tcctttagat cctaaaaatc cgtcagatgt tcgtgcagct gagttggaag
     7621 atattattca caataaattt atcttggatg caacttatct aggtcgctat tcagctgaaa
     7681 cgatggaagg tgtcaaccat atcttatcag tcaatggtgg tagcttggat cttcgtgaag
     7741 aagattttac agcattagaa gcagcaaaag acttgaatga tttccttgga atcaactact
     7801 atatgagtga ctggatggaa gcctttgatg gagaaactga aattatccat aatggtaagg
     7861 gtgagaaagg aagttctaaa taccaaatca aaggtgttgg tcgtcgtgta gctcctgatt
     7921 atgtaccacg tacggattgg gattggatta tctaccctca aggtttatat gaccaaatta
     7981 tgcgtgtgaa gaaagattat cctaactaca agaagattta catcactgaa aatggtctcg
     8041 gctataaaga tgagttcgtc gataacactg tttatgatga tggacgtatt gattatgtga
     8101 aacaacactt ggaggtcttg tctgatgcga ttgcagatgg agcgaatgta aaaggttact
     8161 tcatttggtc attgatggac gtcttttcat ggtcaaatgg ttatgaaaaa cgttacggtc
     8221 tcttctatgt agactttgaa actcaagaac gttatccgaa gaaatcagct cactggtaca
     8281 agaaagtagc ggaaactcag attatagact agtagaatta gtcattagat atagaatttt
     8341 agtgagtcaa aaagatgttc aaagatttta tccaatctat ttatgaaaaa gtttatatta
     8401 taaatttcga aaaatgctct caaataccgt gtttgacgag tgaagaattg aaaagtctat
     8461 ttttaaattt tatcaatcct gaagaatggg atactatctc ctttgattca gattttatgc
     8521 catttcaaca atcgtaacca atttctcaaa aaagttaaat cttatattta gtactctgca
     8581 aaactcttat ctaatcacgt tgcttatact caatgaaaat caaagagcaa ctttaaacta
     8641 ggaagcgagt cgcagatttc tcaatgcata gctttgagga attgggcaaa aagtctttga
     8701 tatagaaaaa gcatagtatc aggtgtttca acacctgata ctatgctttt tattgtggga
     8761 agatttactt tttttcttct gaaattgagt tgttacccag gctctttcag tttattaagg
     8821 cttgatgact ttaatgtgtt tagatagctt aaaaaggatt gaatcactta gtttagaatc
     8881 tgaaacaata gtatcaagat ttgatacatt ataaaaagta taaaaatcaa acttattgaa
     8941 cttactatga tctgcgagta aatatttttt attagaatta tttaaagcga tgcgttgagc
     9001 ctctccctct tcctcgctaa aagtagctag agctccgttt tgaataccat tacagctaac
     9061 gaaagcttta gaaaattgga gattagagag attttgtagg gtcaatgtgc caacaaaagc
     9121 acctgtaata tcgcgataat ttccacctat caaaatcaaa tctgttaatt ttcgttcgct
     9181 taaaatcaga aaaacaggta gactgttggt tacgacgcgg atattgtcaa taggcaactc
     9241 acgcgcaaaa aactctaatg ttgttcctgg tccaatgaaa atagtttctc tttcttctac
     9301 tagactgcct gcaaaatggg ctatttcttg tttttctgcc gtttggaggg cttgtttttc
     9361 aatatttgat cgctcattag tcaaaaggga gttggttcga agtttttcag ctccaccatg
     9421 cacacgaatc agcaaatctt tatcagctaa ttcctgtaaa tagcgccttg cagtcatatc
     9481 tgaaacggct atttcgtcca taatctgttt aactgttatg gttcctttac tatttactat
     9541 ctctaaaatt ttggctaatt tttcttgttt gagcatatta tcacctcgtt tcctactact
     9601 atcttaccat aaacaaactc atcattcaaa tacaaaaaca acaaaatgaa acaaaaacaa
     9661 aaatatcgaa gtttgttttc aaaactttcg atatttttgt tgggttataa ctttgatgtt
     9721 tctagtttac tttttgatga ttgagagtga tggagaatta gtctaaaccg tagttatagt
     9781 catcgtcttg catggcttca acttcgccaa gaaggtaacc atttccgact tgagagaaga
     9841 agtcatggtt ggaagttcct gttgaaatac cgttcataac gattgggttg acatcttcag
     9901 ctgaatctgg gaaaagtgga tcttgtccca tgttcatgag agctttattg gcattgtagc
     9961 gaaggaaggt tttaacctct tcagtccaac caacaccgtc ataaagactc tctgtgtagc
    10021 cttcttcatt ttcataaaga gtatagagta ggtcgtacat ccattctttg agtttttctt
    10081 gctcttcttc aggcaattca ttgaaaccaa gttggaattt gtaaccaatg taggttccgt
    10141 gaacagactc gtcacgaata atcaatttaa tgatttctgc aacgttggct agtttgttgt
    10201 taccgagata gtagagggga gtgaagaaac cagagtagaa gaggaaggtt tcgaggaaga
    10261 cgctggcaac tttcttttca agtgggctgc cgtttaggta gatttcgttg acaatctcag
    10321 ccttcttttg taggtaagga ttggtattgg tccattcgaa aatttcttca atctcagcct
    10381 tagtattcaa ggtagaaaag attgatgagt aagatttagc gtggacagat tccataaatt
    10441 ggatgttatt gaaaacagct tcctcatgtg gtgtacggat gtctgcgcga agggcttgaa
    10501 ccccagtttc agattgcata gtgtcaagaa gggttaaacc accaaaaact tttccgacca
    10561 agtctttctc tttgttagat agctttctcc agtcatccaa gtcgtttgat aagggaatac
    10621 gtgtatcgag ccaaaattgc tccgtcagtt tttcccaagt tgatttgtcg atgacatctt
    10681 cgatggcatt ccagttaatg gctttgtagt aagtttccat ttaaaatctc tttctgtgtt
    10741 tagtattgcg aactcacaat tatttctact ttaccataat tctataggag tatcgcacaa
    10801 aaagtcggaa gcccgacttt taaaatgtta cataaattat gttatgacat agtagatttg
    10861 attttatcag tgctgcttag ggaaaaataa tgtttctatg ctagaaacta aatcacacag
    10921 ctttcacatt ggttggcgcc gacttctcca ccgtcatctg taaaggtacg gacgtagtag
    10981 atagacttga ttcccttgtt aaaggcatag ttacgaagga tggacaagtc acgtgtcgtt
    11041 tgtttatttt ccctcttcca ttcgtaaagg ccttttggaa tgtcactacg catgaagagg
    11101 gtgagtgaaa gtccttgatc cacgtgttca gtcgcagcag cgtaaacatc gatgacttta
    11161 cgcatatcca tatcgtaggc agaagtgtag taaggaatgg tttctgtaga caagccagca
    11221 gcagggtagt agattttacc aattttcttc tcttggcgtt cttcgatacg ttgcgtaatc
    11281 gggtggatag aagcagaaac gtcgttgata tagctgatag aaccatttgg cgctacagca
    11341 aggcgatttt ggtggtaaag accatcttct tgaaccttgt cgcgaagttc agcccaatca
    11401 gcaacaccag ggataaagac atttttgaag agttctttaa cacggtctga tgttggaaca
    11461 aattcaccag ttacatactt gtcaaagtaa cttccgttag catagtctga tttttcaaag
    11521 ttgtggaagg taataccacg ttcacgcgca atattgtttg actctaccaa ggtccagtag
    11581 ttcataagca taaagtagat gcttgtaaat tcaacagact caggtgaacc atattcaatg
    11641 agttgttggg caaggtagct gtgcagtccc atggcaccga gaccaaaggt gtgggcttgg
    11701 ctatttccat ggtcaatcgt tggtacagct acgatatgtg aactatctgt aacgaaagta
    11761 agggcacgaa ccatagcacg gatagaacga ccaaaatcag gtgaagtcat catgttaacc
    11821 acgttggttg aacccaggtt acatgaaaca tctgttccca tttgaaggaa ttcttgagca
    11881 tcgttgatca agcttggttc ttgaacttga agaatctcag aacacaagtt actcatgata
    11941 atctttccat caacaggatt tgcacggtta gccgtatcga tgttgactac ataaggatag
    12001 ccagactctt gttgcaattt agagatttca gtttccaaat cccgcgcctt gatttttgtc
    12061 ttgcgaatat ttggatttgc gaccaattca tcgtattttt cagtaatgtc gatgtaattg
    12121 aatggcacac cgtattcttt ttctacagag taagggctga agaggtacat ttcttcattt
    12181 ttacgagcca attcgtagaa tttatcaggt actacaacac caagtgatag agtcttgaca
    12241 cgtacttttt catcagcgtt ttctttctta gttgaaagga aagcgatgat atctgggtga
    12301 aagacgttga ggtagacaac accggcacct tgacgttgcc ccaattggtt ggagtaagag
    12361 aagctgtctt caaaaagctt cataacagga acgacacctg aagcagctcc ttcatagcct
    12421 ttgataggtg caccagcttc acgaaggttg ctgagggtaa ttcccacacc accaccaata
    12481 cgtgaaagtt gaagagctga gttgatagaa cgtccgatag agttcatatc atccgtcact
    12541 tggattagga aacaagatac caactctcca cggcgagcac gtccagcatt caagaaggaa
    12601 ggagtagcag gttggtagcg ttggtggatg atttcattgg caatatcgat tgcaacagct
    12661 tcattcccat cagcgaaata aagggcgttg aagaagacac ggtcttccat attttcaaga
    12721 tagtattcac cgtcattagt ctttaaggca tattgattgt aaaatttata agctgccatg
    12781 aatgacttga attggaagtt ttggtctttg ataaattgag ctaattcttc caagaactct
    12841 ggacggtatt tcttgataaa ggctgtttcg atgtagttgt gttcaatgag gtaattgatt
    12901 ttgtctttga ttgaatcaaa aaccatagtg tttggaacta cattttcttt aaagaaagca
    12961 tccaaggctt ccttgtcttt atgaagcatg atttgtccat taacaggacg gttaatttcg
    13021 ttattaagac ggaagtaagt cacgtcttca agatgtttta atcccataaa atttccctta
    13081 tctaattaca aaagaaaggc ttctaagtta gccctaaaag cagtttcttc tggatgatgt
    13141 actaagatta tgctaattgt ttcagttttc ctggttggaa acctgaaaag acttcagttg
    13201 gtgtttggat aacaggagct gcgctaaaac cgagctcttt aacttgatcg acgtactcag
    13261 gttgctcatc aagattgatt tcacgataag agacattatt actgtccaag aaacgcttgg
    13321 tcattttaca ttggacacaa ttgtttttag aataaacggt taccattgtg taactcctct
    13381 tcaaaattta atactatctt agtatatcag aaaataaaat tttgtcaatg atttgaacta
    13441 aatatagtgg tgttcttttg ttgtttttat atataaacac aatatatagt gtttgtattg
    13501 tgaaatagca atgattatcc tataaattgt ttttaaagaa actatatcct gtgttttgtt
    13561 atctaggata gaagattttc agcaagttat ctgaaaggaa attgaaggaa aatccagaaa
    13621 atgcttgaaa aaaatcctag aagatggtat aatactaaat tgtaagggtt atcacatata
    13681 actcaaaaaa agaaagaaca aaaggagagt caaactatgg cttctaaaga tttccacgta
    13741 gtggcagaaa caggtattca cgcacgtcca gcaacattgt tggtacaaac tgctagcaaa
    13801 tttgcttcag atatcactct tgagtacaaa ggtaaatcag ttaaccttaa atcaattatg
    13861 ggtgttatga gtcttggtgt tggccaaggt gctgacgtaa ctatctcagc tgaaggtgca
    13921 gatgcagatg acgctatcgc tgcaatctca gaaacaatgg aaaaagaagg attggcataa
    13981 gggaaatgac agaaatgctt aaaggaatcg cagcatctga cggtgttgca gttgcaaaag
    14041 catatctact cgttcagccg gatttgtcat ttgagactat tacagtcgaa gatacaaacg
    14101 cagaagaagc tcgccttgat gccgctctac aggcatcaca agacgagctt tctgttattc
    14161 gcgagaaagc agtaggtacg ctcggtgaag aagcagctca agtttttgat gctcacttaa
    14221 tggttcttgc tgacccagaa atgatcagcc aaatcaagga aactatccgt gcgaagaaag
    14281 tgaatgcaga agcaggtctg aaagaagtta cagatatgtt tatcactatc tttgaaggca
    14341 tggaagacaa cccatacatg caagaacgcg cagcggatat ccgcgacgtg acaaaacgtg
    14401 tattggcaaa ccttcttggt aaaaaattgc caaacccagc ttctattaat gaagaagtga
    14461 ttgtgattgc gcatgacttg acaccatctg atacagctca attggacaaa aactttgtaa
    14521 aagcttttgt aaccaacatt ggtggacgta caagccactc agctatcatg gcacgtacac
    14581 ttgaaattgc tgctgtatta ggtacaaata acatcactga aatcgttaaa gacggtgaca
    14641 tccttgctgt taacgggatc actggagaag tgattatcaa cccaacagat gaacaagcgg
    14701 cagaatttaa agcagctggt gaagcctatg cgaaacaaaa agctgaatgg gcacttttga
    14761 aagatgctca aacagtgact gctgacggta aacacttcga gttggctgcc aatattggta
    14821 ctccaaaaga cgttgaaggt gttaacaaca acggtgcaga agctgttgga ctttaccgta
    14881 cagagttctt gtacatggat tctcaagact tcccaactga agatgagcag tatgaagcat
    14941 acaaggctgt tcttgaagga atgaacggta aacctgttgt cgttcgtaca atggatatcg
    15001 gtggagataa ggaacttcct tacttcgata tgcctcacga aatgaaccca ttccttggat
    15061 tccgtgctct tcgtatctct atctctgaga ctggagatgc tatgttccgc acacaaatcc
    15121 gtgctcttct tcgtgcgtct gttcacggtc aattgcgtat catgttccca atggttgcgc
    15181 tcttgaaaga attccgtgca gcgaaagcag tctttgatga agaaaaagca aaccttcttg
    15241 ctgaaggtgt tgcagttgcg gataacatcc aagttggtat catgatcgag attcctgcag
    15301 cggctatgct tgcagaccaa tttgctaaag aagttgactt cttctcaatt ggtacaaacg
    15361 acttgatcca atatacaatg gcagcagacc gtatgaacga acaagtttca tacctttacc
    15421 aaccatacaa cccatcaatc ctacgcttga ttaacaatgt gatcaaagca gctcacgctg
    15481 aaggtaaatg ggctggtatg tgtggtgaga tggctggtga ccaacaagct gttccacttc
    15541 ttgtcggaat gggcttggat gagttctcta tgtcagcaac atctgtactt cgtacacgca
    15601 gcttgatgaa gaaactcgac acagctaaga tggaagagta cgcaaaccgt gcccttacag
    15661 aatgctcaac aatggaagaa gttcttgaac ttcaaaaaga atacgttaat tttgattaat
    15721 cgaaaagtcc ctgcaactca gttacaggga ctttttttga tattttaaaa agaattttca
    15781 agaaaatctt tcttataaaa agtccaacct tgaaaaagta gtggtcagaa caaaaaatac
    15841 ttaaatggtt cataaaattc ttgacaagtt ggagatttag gagtaaacta ttaaccagtt
    15901 aagtaataga gaggagtttc tgcaatttag aaatgaattg caactagaaa tatcaaatag
    15961 aaagagagtt tcgatgaaaa ttaataagaa ataccttgtt ggttctgcag cagctttgat
    16021 tttaagtgtt tgttcttacg agttgggact gtatcaagct agaacggtta aggaaaataa
    16081 tcgtgtttcc tatatagatg gaaaacaagc gacgcaaaaa acggagaatt tgactcctga
    16141 tgaggttagc aagcgtgaag gaatcaatgc tgagcaaatc gtcatcaaga taacagacca
    16201 aggctatgtc acttcacatg gcgaccacta tcattattac aatggtaagg ttccttatga
    16261 tgcgattttc agtgaagaat tactcatgaa agatccaaac tataagctaa aagatgagga
    16321 tattgttaat gaggtcaagg gtggatatgt tatcaaggta gatggaaaat actatgttta
    16381 ccttaaggat gctgcccacg cggataacgt ccgtacaaaa gaggaaatca atcgacaaaa
    16441 acaagaacat agtcaacatc gtgaaggtgg gactccaaga aacgatggtg ctgttgcctt
    16501 ggcacgttcg caaggacgct atactacaga tgatggttat atctttaatg cttctgatat
    16561 catagaggat actggtgatg cttatatcgt tcctcatgga gatcattacc attacattcc
    16621 taagaatgag ttatcagcta gcgagttggc tgctgcagaa gccttcctat ctggtcgggg
    16681 aaatctgtca aattcaagaa cctatcgccg acaaaatagc gataacactt caagaacaaa
    16741 ctgggtacct tctgtaagca atccaggaac tacaaatact aacacaagca acaacagcaa
    16801 cactaacagt caagcaagtc aaagtaatga cattgatagt ctcttgaaac agctctacaa
    16861 actgcctttg agtcaacgac atgtagaatc tgatggcctt gttttcgacc cagcgcaaat
    16921 cacaagtcga acagctagag gtgttgcagt gccacacgga gatcattacc acttcatccc
    16981 ttactctcaa atgtctgaat tggaagaacg aatcgctcgt attattcccc ttcgttatcg
    17041 ttcaaaccat tgggtaccag attcaaggcc agaacaacca agtccacaac cgactccgga
    17101 acctagtcca ggcccgcaac ctgcaccaaa tcttaaaata gactcaaatt cttctttggt
    17161 tagtcagctg gtacgaaaag ttggggaagg atatgtattc gaagaaaagg gcatctctcg
    17221 ttatgtcttt gcgaaagatt taccatctga aactgttaaa aatcttgaaa gtaagttatc
    17281 aaaacaagag agtgtttcac acactttaac tgctaaaaaa gaaaatgttg ctcctcgtga
    17341 ccaagaattt tatgataaag catataatct gttaactgag gctcataaag ccttgtttga
    17401 aaataagggt cgtaattctg atttccaagc cttagacaaa ttattagaac gcttgaatga
    17461 tgaatcgact aataaagaaa aattggtaga tgatttattg gcattcctag caccaattac
    17521 ccatccagag cgacttggca agccaaattc tcaaattgag tatactgaag acgaagttcg
    17581 tattgctcaa ttagctgata agtatacaac gtcagatggt tacatttttg atgaacatga
    17641 tataatcagt gatgaaggag atgcatatgt aacgcctcat atgggccata gtcactggat
    17701 tggaaaagat agcctttctg ataaggaaaa agttgcagct caagcctata ctaaagaaaa
    17761 aggtatccta cctccatctc cagacgcaga tgttaaagca aatccaactg gagatagtgc
    17821 agcagctatt tacaatcgtg tgaaagggga aaaacgaatt ccactcgttc gacttccata
    17881 tatggttgag catacagttg aggttaaaaa cggtaatttg attattcctc ataaggatca
    17941 ttaccataat attaaatttg cttggtttga tgatcacaca tacaaagctc caaatggcta
    18001 taccttggaa gatttgtttg cgacgattaa gtactacgta gaacaccctg acgaacgtcc
    18061 acattctaat gatggatggg gcaatgccag tgagcatgtg ttaggcaaga aagaccacag
    18121 tgaagatcca aataagaact tcaaagcgga tgaagagcca gtagaggaaa cacctgctga
    18181 gccagaagtc cctcaagtag agactgaaaa agtagaagcc caactcaaag aagcagaagt
    18241 tttgcttgcg aaagtaacgg attctagtct gaaagccaat gcaacagaaa ctctagctgg
    18301 tttacgaaat aatttgactc ttcaaattat ggataacaat agtatcatgg cagaagcaga
    18361 aaaattactt gcgttgttaa aaggaagtaa tccttcatct gtaagtaagg aaaaaataaa
    18421 ctaatgaaaa atgaaagtct cgataaagag gctttcattt ttattatgta tatatgtaaa
    18481 attcttgaca agcaatatta aaaagagtaa actattaact agttaattaa ccggtttatt
    18541 actttatagt gaatcaaata tacttaagaa aagaggaaag aatgaaaatc aataaaaaat
    18601 atctagctgg gtcagtagct acacttgttt taagtgtctg tgcttatgaa ctaggtttgc
    18661 atcaagctca aactgtaaaa gaaaataatc gtgtttccta tatagatgga aaacaagcga
    18721 cgcaaaaaac ggagaatttg actcctgatg aggttagcaa gcgtgaagga atcaatgccg
    18781 aacaaatcgt catcaagata acagaccaag gctatgtcac ttcacatggg gaccactatc
    18841 attattacaa tggtaaggtt ccttatgacg ctatcatcag tgaagaattg ctgatgaagg
    18901 atccaaacta ccagctaaag gacgaggata ttatcagtga aatcaagggt ggttatgtga
    18961 ttaaggtaga tggaaaatac tatgtttacc ttaaggatgc agctcatgcg gataatgtcc
    19021 gtacaaaaga agaaatcaat cggcaaaaac aagaacatag tcagcatcgt gaaggaggga
    19081 cttcaacaaa cgatggtgcg gtagcctttg cacgttcaca aggacgctat actacagatg
    19141 atggttatat ctttaatgct tctgatatca ttgaggacac gggtgatgct tatatcgttc
    19201 ctcacggcga ccattaccat tacattccta agaatgagtt atcagctagc gagttagctg
    19261 ctgcagaagc cttcctatct ggtcggggaa atctgtcaaa ttcaagaacc tatcgccgac
    19321 aaaatagcga taacacttca agaacaaact ggctaccttc tgtaagcaat ccaggaacta
    19381 caaatactaa cacaagcaac aacagcaaca ctaacagtca agcaagtcaa agtaatgaca
    19441 ttgatagtct cttgaaacag ctctacaaac tgcctttgag tcaacgccat gtagaatctg
    19501 atggccttgt tttcgaccca gcgcaaatca caagtcgaac cgccagaggt gtagctgtcc
    19561 ctcatggtaa ccattaccac tttatccctt atgaacaaat gtctgaattg gaaaaacgaa
    19621 ttgctcgtat tattcccctt cgttatcgtt caaaccattg ggtaccagat tcaagaccag
    19681 aacaaccaag tccacaatcg actccggaac ctagtccaag tctgcaacct gcaccaaatc
    19741 ctcaaccagc tccaagcaat ccaattgatg agaaattggt caaagaagct gttcgaaaag
    19801 taggcgatgg ttatgtcttt gaggagaatg gagtttctcg ttatatccca gccaaggatc
    19861 tttcagcaga aacagcagca ggcattgata gcaaactggc caagcaggaa agtttatcgc
    19921 ataagctagg agctaagaaa actgacctcc catctagtga tagagaattt tacaataaga
    19981 cttatgactt actagcaaga attcaccaag atttacttga taataaaggt cgacaagttg
    20041 attttgaggc tttggataac ctgttggaac gactcaagga tgtctcaagt gataaagtca
    20101 agttagtgga tgatattctt gccttcttag ctccgattcg tcatccagaa cgtttaggaa
    20161 aaccaaatgc gcaaattacc tacactgatg atgagattca agtagccaag ttggcaggca
    20221 agtac
//
LOCUS       DAVBGY010000042        17572 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVBGY010000042 DAVBGY010000000
VERSION     DAVBGY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17572
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.042"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(179..1114)
                     /locus_tag="VQ620_001447"
     CDS             complement(179..1114)
                     /locus_tag="VQ620_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HET2764052.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(1111..1971)
                     /locus_tag="VQ620_001448"
     CDS             complement(1111..1971)
                     /locus_tag="VQ620_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET2764053.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFYYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            2079..4241
                     /locus_tag="VQ620_001449"
     CDS             2079..4241
                     /locus_tag="VQ620_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET2764054.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATITSYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIILSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYFYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4333..5592
                     /locus_tag="VQ620_001450"
     CDS             4333..5592
                     /locus_tag="VQ620_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2764055.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADDTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRSE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5606..6472
                     /locus_tag="VQ620_001451"
     CDS             5606..6472
                     /locus_tag="VQ620_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2764056.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            6486..7319
                     /locus_tag="VQ620_001452"
     CDS             6486..7319
                     /locus_tag="VQ620_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2764057.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKSTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIIAGISNGAV
                     K"
     gene            7320..7400
                     /locus_tag="VQ620_001453"
                     /pseudo
     CDS             7320..7400
                     /locus_tag="VQ620_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            7538..8980
                     /gene="gtfA"
                     /locus_tag="VQ620_001454"
     CDS             7538..8980
                     /gene="gtfA"
                     /locus_tag="VQ620_001454"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HET2764058.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTEILPEIHEHYSIQFKIADHDYYVYDFTLPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAREVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVSF"
     gene            9118..10311
                     /locus_tag="VQ620_001455"
     CDS             9118..10311
                     /locus_tag="VQ620_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET2764059.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            10998..12977
                     /locus_tag="VQ620_001456"
     CDS             10998..12977
                     /locus_tag="VQ620_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2764060.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13044..14540
                     /locus_tag="VQ620_001457"
     CDS             13044..14540
                     /locus_tag="VQ620_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2764061.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14540..15466
                     /locus_tag="VQ620_001458"
     CDS             14540..15466
                     /locus_tag="VQ620_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2764062.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSIRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15475..16542
                     /locus_tag="VQ620_001459"
     CDS             15475..16542
                     /locus_tag="VQ620_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2764063.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16553..17479
                     /locus_tag="VQ620_001460"
     CDS             16553..17479
                     /locus_tag="VQ620_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2764064.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
BASE COUNT         5174 a         3141 c         3676 g         5581 t
ORIGIN      
        1 aggctccata atatccatag ggaatttacc cactacaaat attatagagc ccgttttaat
       61 gagagtttgt aactctcttt tttgttttta gagacaataa aacttcagat ccgtaaaact
      121 taattgaatt actaatctga agttattttt atatcgttta tattataatt gtgttatttt
      181 acttccaact attgagagaa atttcgccac tatttagcca gatttctttt ccgttatcac
      241 attgaactaa aagttttcca ttttctgaga tgtctttagc aagtcccttg tagtcttttt
      301 gctctagtgt gaaagtgact tcttttccta gaatgaatga ctgttttttg tataggtata
      361 atagctcttc tgctggtgtt tcgaagaaag cacgccagat ttctatgatc aattcattcc
      421 ttgttatagg agctgtagct ttaaataagc tggcagcttt ttcttttaat tcctgaggga
      481 agtctttaat agtgaaattg atacctactc caataatgat atctgtgact aagccagttt
      541 ctacagaggt cattgcttca gtaaggattc ctccaatttt atgattgttt agatagatat
      601 cattgaccca ttttatgtcg acatctatta aagttaggtt cttaatggct ttgtagacag
      661 ctccagctac aagtagtgtg taggatggta atttgtcata ggggagattt ggtttaagat
      721 ggagtgtcat ataaatacca ccttgtggtg agtagaagga acgttgaaaa cggcctcggc
      781 ctgctgtttg ataggaagct agatagaggg tatttgcttc atggcctaaa tcaattgctt
      841 cttttgcatc tagttgtgtt gattttgttt cgggtttaaa gctgacttta attggaagat
      901 tttcttctag aatctctgga agaataaggt caccattcat cagtttatat cctctatttt
      961 tgatactatc aatttcaatg ccttcttgtt ctagtcgctt gatggctttc caaattgctg
     1021 ttcggcttag ggatagtttt tctgcgattt tttctccgct gatatagtcg gtttctttag
     1081 ataggatttg gtagacagct tggtaggatt tcataatgtt gcctttctca ctagttggta
     1141 ttgagagtat tcttttcttg tatgacttgg agtctgatta aagtattgtt tataagcttt
     1201 cgaaaaatgg agtggatctg aaaaacctac cgagtatgca attaccttga tggactcttg
     1261 ggtattttcg agaagttgtc tagctcggtg cattcgaacg tagagtaggt attctttggg
     1321 tgataaggta ttaaattctt tgaatacgct tgataagtag cttctgtgaa cggatagttc
     1381 ttttgctaaa tcttgaattg taagtgattg aggatagtgg ctatcaatta atcgtttgca
     1441 ttcaagatag agttggtggg ttgatgaaat attctttttt ttctgattgg gagcaatagt
     1501 tcccagatga aacatcagtt catgaagttg tcccatgata tggagttgag ctaattcact
     1561 tgattttgta atctgagcga agcggacaat gtctgagatg agttttgcag tagtctgggt
     1621 atgacaagtt tcagattgga tgagatagga ttgatcagaa atttgagaaa gagcaaaata
     1681 atcaggggat ttccctccag tgattcctaa ccagtagtag gcccaaggtt ctttactatc
     1741 tgcttgataa aaggttagtt cctctggttt taatagaaag aaatctcctt cttttaaatc
     1801 aacaatttta cccttgtaat aaaattttcc ttgtccttta gtaatgtaat gtagaacgta
     1861 tgtatcacga atggctggac caaaagagta attaggtgtg cattcctcat atccataaaa
     1921 gcttagggca aggtcgattg ttccagtctg gtattctgaa aaaactaaca tgtgctacct
     1981 cctacctaac attttaccat attcttgaga catttttcta ttttggaaag cgatttcagg
     2041 tgataaaata taatcaataa agtgatgagg tgaagtaaat gggagttagg atagagaata
     2101 atctatttta tgttgagagt aaaaatctaa gtttgattat tgaaaatcga aatggctact
     2161 tacttttgaa acatttagga aagactatta agaactataa aggttccaat agtgtttatg
     2221 aacgagacca tgccttttca ggaaatccaa cggctactaa tcgaaccttt agtttagata
     2281 ctcagcgaca gatttttgga caacatggct taggagattt taggaaacca actatacagg
     2341 ttcagcatag tgtaactgaa gtaacagact ttcgatttgt agaagcaaag attttaaaag
     2401 gtcagaatgg tccacagggc ttaccttctc cacatagcat ggacgataca gagactcttg
     2461 tcttaatgtt agaagattct aaggctcaac ttagtctgac tttgtattat actactttta
     2521 ataatgatgc gactattact agctacagta aattagataa taatagtaat caggaagttg
     2581 tcatccataa agatttttct tttatggctg attttccagc tgcagattac gaaatagcaa
     2641 ctctgcaggg tgcttatgct cgtgaaaaga ctgttagacg tcaacaggta gaacaaggaa
     2701 tcttttcgat tagttcaaac cgaggtgctt ctggtcatgc tcaaacacca gctcttctac
     2761 tatgcgaaca aggagtcaca gaggatgctg ggaatgtgtt tgctattcaa ctaatgtata
     2821 gtggcaactt tgaagctttt gttcaaaaaa atcaattgaa tgaagttcgg gtggctattg
     2881 gcattaatcc agaaaacttt tcttggaagt tagcccctga ggaatacttt gaaacaccgg
     2941 tagctttagt gactcattca gatcagggat taactggtat tagtcatgaa agtcagaatt
     3001 ttgtactgaa gcacattatt ctaagtgaat tttctaaaaa agaacgtcca attctaatca
     3061 ataactggga agctacttac tttgactttc agagagaaaa actgttagag ttagcagatg
     3121 aagctaagaa agttggcatt gaactttttg tattagatga tggttggttt ggcaatcgtt
     3181 ttgatgataa tcgtgcttta ggtgattggg ttgttaatga ggaaaaactg ggtggaagtc
     3241 tagaaagtct gatttcagct atccatgaaa gaggtttgca gtttggactt tggttagaac
     3301 ccgaaatgat ttctgtagat agtgatttgt atcgtcaaca ccctgactgg gctattcagg
     3361 ttcctgacta tgagcatact tattctcgga atcaattagt acttaatctt gccaatcctc
     3421 aggtagtaga atacttgaaa agtgtcttag atcaactcct atcttatcat gagattgatt
     3481 acattaaatg ggatatgaac cgcaatatca ctaagctagg gaatggatta acttatctag
     3541 agacacagat gcaatctcat cagtacatgc tggggcttta tgaactcgtt tcttatctga
     3601 cagagaagca cagccatatt ctctttgagt cctgctctgg tggtggtgga cgaaatgatc
     3661 ttggtatgat acgctatttc ccacaagtct gggctagtga taatactgat gccattgcac
     3721 gtttaccaat tcaatacggt tcatcctatt tctatccaac catttctatg ggggctcatg
     3781 tgtcagcagt accgaatcat cagatgggac gaatgacacc attagaaaca cgtggccatg
     3841 tagcaatgat gggaaatttg ggctatgagc ttgatttgac aaatttatca gatgaagaga
     3901 aagctacgat tgctaatcag gtgaacttgt ataaagaatt acgaccagta gttcagttag
     3961 gacagcagta tagactaatt aatcctgata ctgcatccaa tgaagcagct gtacaattta
     4021 attacggaaa tcaaacgatt gtaacctacg ttcgcgtttt atctgttgta gagaccatgg
     4081 aaacaacttt aaaattaaaa gatttggatg aagagggact atataaatta caggaaaatg
     4141 gcgaagttta ctcaggtgca gaactaatgt atgcgggctt aactgttatc ttatcccaag
     4201 gagatttttt gagtagacag tatattttta gaaaactatg atatgtactg accccaaaag
     4261 ttagatttat tctatctaac ttttgaagtg cagtttgata agggtgtata aatttataaa
     4321 ggaggctttc taatggaatg gtataaaaaa atcggacttc ttgcaactac aggtttagct
     4381 ttgtttgggc tcggtgcttg ctccaactat ggtaaatctg cggatgacac agtgaccatc
     4441 gagtatttca accagaaaaa agaaatgacc aaaaccttgg aagaaatcac tcgtgatttt
     4501 gagaaggaaa accctaagat aaaggtcaaa gtcgtcaatg taccaaatgc tggtgaagta
     4561 ttgaagacac gcgttctcgc aggagatgtg cctgatgtgg tcaatattta cccacagtcc
     4621 atcgaactgc aagaatgggc aaaagcaggt gtctttgaag atttgagtaa caaagactac
     4681 ctgaaacgcg tgaaaaatgg ctacgctgaa aaatatgctg taaacgaaaa agtttacaac
     4741 gttcctttta cagctaatgc ttatggaatt tactacaaca aagataaatt cgaagaactg
     4801 ggcttgaagg ttcctgaaac ctgggatgaa tttgaacagt tagtcaaaga tatcgttgct
     4861 aaaggacaaa caccatttgg aattgcaggt gcagatgctt ggacactcaa tggttacaat
     4921 caattagcct ttgcgacagc aacaggtgga ggaaaagaag caaatcaata ccttcgttat
     4981 tctcaaccaa atgctattaa attgtcggat ccgattatga aagatgatat caaggtcatg
     5041 gacatccttc gcattaatgg ctctaagcaa aagaactggg aaggtgctgg ctataccgat
     5101 gttatcggag ccttcgcacg tggggatgtc ctcatgacac caaatgggtc ttgggcgatc
     5161 acagcgatta atgaacaaaa accgaacttt aagattggaa ccttcatgat tccaggaaaa
     5221 gaaaaaggac aaagcttaac cgttggtgcg ggagacttgg catggtctat ctcagccacc
     5281 accaaacatc caaaagaagc caatgccttt gtggaatata tgacccgttc agaagtcatg
     5341 caaaaatact acgatgtgga cggatctcca acagcgatcg aaggggtcaa acaagcagga
     5401 gaagattcac cgcttgctgg tatgaccgaa tatgccttta cggatcgtca cttggtctgg
     5461 ttgcaacaat actggaccag tgaagcagac ttccatacct tgaccatgaa ctatgtcttg
     5521 accggtgata aacaaggcat ggtcaatgat ttgaatgcct tctttaaccc gatgaaagcg
     5581 gatgtggatt aggagtagag agactatgaa aaaagtatta caaaaatatt gggcatgggc
     5641 ttttgtggtc atccccctct tgttacaagc aattttcttc tatgtgccga tgtttcaagg
     5701 agccttttac agttttacca actggacagg attgacttac aactacaaat ttgtcggctt
     5761 aaacaacttt aagctcctct tcatggatcc aaaattcatg aatgcgattg gctttaccgc
     5821 aatcattacg attgccatgg tggttggtga gattgcactc gggatcttca ttgcgcgtgt
     5881 cttgaactct aaaatcaaag gccaaacctt cttccgtgct tggttcttct tcccagctgt
     5941 tttatctggt ttgacagtgg ctttgatctt caagcaagtc ttcaactacg gtcttccagc
     6001 gattgggaat gcccttcata ttgaatttct ccaaaccagt cttttaggga ctaagtgggg
     6061 agcaatcttt gcggctgtct ttgtccttct ttggcaaggg gtggctatgc ccatcatcat
     6121 cttcctagct ggtttgcaat ctattccaac tgagattaca gaggcagcaa ggattgatgg
     6181 tgcgactagc aagcaagttt tctggaacat tgaattgcct tacttgctac caagtgtctc
     6241 tatagtcttt atcctagccc taaaaggtgg gctgactgcc tttgaccaag tctttgccat
     6301 gaccggtggt ggtccaaaca atgccacaac ctcacttggg ctcttggttt ataactatgc
     6361 ctttaaaaac aaccaattcg gttatgccaa tgccattgcc gtaatcttgt tcttcttaat
     6421 tgtagtgatt tcgatcatcc aattgagagt atctaagaaa tttgaaattt aagaggagaa
     6481 gcatgatgaa acaagatgaa agaaaagccc tgattggcaa atacattcta ttgattctag
     6541 gatcggttct gattttagtg ccgctccttg ctaccctctt tagttccttc aaatccacta
     6601 aggatattgt agataatttc tttggctttc caaccaactt cacatgggac aactttagcc
     6661 gtctcttagc tgatgggatt ggaggctatt attggaactc tgtcgtcatc actgtcttgt
     6721 ctttacttgc agtaatgatc tttatcccta tggcagccta ctccatcgct cgcaatatga
     6781 gtaaaagaaa agcctttacc atcatgtata ccctcttaat cctcggaatc ttcgtacctt
     6841 tccaagtcat catgattccg attacggtta tgatgagtaa actcggtttg gctaatacct
     6901 ttggtttgat cttgctctac ttgacctatg cgattccaca gaccctcttt ctctatgttg
     6961 gctatatcaa aatctcgatt ccagaaagtc tggatgaagc agcagagatc gatggggcta
     7021 atcaatttac aacctatttc cgcatcatct tcccaatgat gaaaccgatg catgcgacaa
     7081 ccatgatcat caatgccctt tggttctgga atgacttcat gttgccactc cttgtcttga
     7141 accgggattc caaaatgtgg actctgcctt tgttccaata caactacgca ggccaatatt
     7201 tcaacgacta cggaccaagc tttgcctctt acgtggtcgg cattatcagt atcaccattg
     7261 tctatctctt cttccaacgc catatcatcg caggaataag caacggggca gtgaagtaat
     7321 actcaatgaa aatcaaagag caaactagga agctagccgc aggttgctca aaacactgtt
     7381 ttgaggttgt ggatgaaact gacgaagtca gtaaccatat ctacggtaag gcgacactga
     7441 cgtggtttga agagattttc gaagagtata acatgaattt gatttacgaa gccagatcat
     7501 atgagacttg gcttcaatta gaaaaaggag agtaatgatg ccaattcaaa ataaaaccat
     7561 gttgattacc tattctgata gccttggaaa taatcttaaa gacttatatg ataatttgga
     7621 agagcatttt ggagatgcta ttggaggagt tcacctttta ccatttttcc catcaacagg
     7681 tgatcgtgga tttgcgccag ttgactacga cgaagtggac tcagcttttg gtgattggga
     7741 ggatgttaag cgtttaggtg agaaatatta tcttatgttt gattttatga ttaatcatat
     7801 ttctcgtcaa tccaagtatt ataaggacta tcaagaaaaa catgaagcca gtgaatttaa
     7861 agatctcttt ttaaactggg ataagttttg gccagaaaac cgtccgacac agtctgatgt
     7921 agatttaatt tacaagcgta aggatcgtgc accaaagcaa gagattgtgt ttgaagatgg
     7981 ttcagtggaa catttgtgga atacctttgg tgaggagcag attgatcttg atgtgaccaa
     8041 agaagtaact atggaattta tccgtaagac cattcagcac ttggcaagta atgggtgtga
     8101 tttgattcgt ctagatgcct ttgcttatgc agtgaagaaa ttggatacta atgatttctt
     8161 tgtggaacca gatatttggg atttattgga caaagttcga gatatcgctg ctgagtatgg
     8221 gacagagatt ttacctgaga ttcatgaaca ctattcgatt cagtttaaaa tagcagacca
     8281 tgattactat gtttatgatt ttactcttcc aatggtgaca ctttatactc tttacagttc
     8341 cagaacagag cgtttggcta agtggttgaa gatgagcccg atgaagcaat ttacgacgct
     8401 agatacccat gatgggattg gagtagtaga tgtcaaggat atcctgaccg atgaggagat
     8461 tgactatgct tcaaatgaac tctataaggt tggagccaat gtcaaacgta agtactctag
     8521 tgccgagtat aacaacttag atatctacca aatcaattca acctactatt cagcgcttgg
     8581 agatgatgat gtcaagtatt ttctcgctcg tctaattcaa gcttttgccc caggtattcc
     8641 tcaggtttac tatgtgggtc tattagcagg caagaatgac ttgaaattat tagaagaaac
     8701 taaagaaggt cgaaatatta atcgtcatta ctatagcaac gaggaaatag caagagaagt
     8761 gcaacgacct gttgtgaagg cccttctcaa tctattttct ttccgtaacc gttcagaagc
     8821 ctttgatcta gaagggacta ctgagataga gacaccaaca gctcacagca ttgtaatcaa
     8881 acgtcaaaat aaagataagt ccgtaacagc agtagcagaa attgatttgc aaaatcagac
     8941 ttatcgggta attgagaatg gagttgaagt atcattttga agccttgata tggtggataa
     9001 aatagggttt ttattttgga aaacgtttcc tttgttttca aattgctaaa aaagtggtac
     9061 aatataggat agcttacgat tatctgaatc agctgatttg gagagaaagg attcattttg
     9121 aaatcaatag gctttattga aaagctgaag gggttgtcta gtaaagagct gattttattg
     9181 ggaattatcc taagtatctt tttacccttt tatctttttg tagttgtact ctgtttatat
     9241 attatcagtt tgatttttac aggagacatg aaaagtattc ttcagaaaat gggggagcat
     9301 ccgatgctgc ttctttttct tagctatagt actgttatat ccattcttgc acaaaattgg
     9361 atgggtcttg tggcttcagt aggaatgttt ctatttacta ttttcttttt gcactatcag
     9421 tcgattttat cccataaatt ctttcgattg attttgcagt tcgtcttgtt tggtagtgtc
     9481 ttgtcagctg cttttgccag tttagaacat ttccaaattg tgaagaaatt taactatgct
     9541 tttctttcac ccaatatgca ggtgtggcat cagaaccggg cagaagtgac cttctttaat
     9601 cctaattatt atggaattat ttgttgtttc tgtattatga ttgctttcta tctgtttaca
     9661 acgaccaagt tgaattggtt gaaagtattc tgtgtgattg caggctttgt taatctcttt
     9721 ggtttgaact ttactcaaaa tcgaactgcc tttcctgcta ttatcgctgg agcaattatc
     9781 tatctcttta cgactattaa aaactggaag gccttttggc ttagtattgg ggtcttcgcg
     9841 attggtttga gtttcctctt ttctagtgat ttgggagttc gaatgggtac tttagactct
     9901 tctatggaag aacgcatttc tatctgggat gctgggatgg ccttgtttaa gcaaaatcct
     9961 ttttggggtg aagggccatt gacctatatg cactcttatc ctcggataca tgctccttat
    10021 catgaacatg cccacagtct ttatattgat acgattctga gttacggaat tgtgggtacc
    10081 attttattag ttttgtcttc tgttgctcct gttcgcttga tgatggatat gagtcaggag
    10141 tcggggaaac gtccgattat cggcctttat ctatctttcc ttacagtggt tgctgtgcac
    10201 ggaatttttg acttggctct cttctggatt cagtcaggct ttattttctt gctagttatg
    10261 tgcagtattc cattggagca tcgaatgttg gtatcggaca tgacggatta agttttataa
    10321 gtttgaaatc ttctaccttg ggtaggagat ttttttatac gatgggcatg tcgtgcatct
    10381 gaggtgtaag tcctctgtag ggatccacta ccggtgaata caatagcgct ccccaagcct
    10441 gactatcgtg aggtagcgga ttaaaatggt ctggggatag atcgttttaa gtctgacgca
    10501 ggaaataaga attgtcagag aaagcaatag tgaaatcgta ggctatgaat agaaacgtga
    10561 tagcgaggag catatagtgg ataaggtagc gagaaatttt aaagtataaa agatagtctt
    10621 aatctatata tgtaaatcac aaaagtaatt gaattcggaa taaggttggt gtgaaaagac
    10681 atagtaaact gaatcttgaa tactagaatg atacttctac aatattgtta taatttaatt
    10741 tgaattccaa aatcacattg ttccgtccgt atttcatttt actgtatgtc ttcctaaact
    10801 ccaaagtttc tgcgtcaaat ttcctatttt cactgtagtc tttgacactt cgaacgacaa
    10861 tttgaatcat atattcaaaa aaaaatgaaa aatttgtgcc aacaagccta aatattctga
    10921 aaatttgttt gatttttaat ggataatgtg atataatggt tgtcaatcta taattcacat
    10981 aaacagagga gtagaaaatg aaaaaaaatc gtgtatttgc tacagcaggt cttgttttat
    11041 tagcagcagg tgtacttgca gcatgcagtt cttcaaaatc atctgattca tcagccccta
    11101 aagcttatgg ctatgtttat acagcagacc cagaaacctt ggactacctg atttcaagta
    11161 aaaatagtac aacagtagtg acttcaaatg ggattgatgg tttattcact aacgataatt
    11221 acggtaatct tgctcctgca gttgcagagg attgggaagt ctctaaggat ggtttgacct
    11281 acacttataa gattcgtaaa ggggttaaat ggtttacctc tgatggagaa gaatatgcag
    11341 aggtgacggc taaagatttc gtgaacggtt taaaacacgc agcagataaa aaatcagaag
    11401 ctatgtattt agctgaaaat tcggttaaag gcttggcaga ttatctatca ggaacttcaa
    11461 cagatttttc aacagttggt gtcaaggcgg ttgatgatta tacgttacaa tacactttga
    11521 accagcctga accgttctgg aactctaagt tgacctattc tattttctgg cctctgaatg
    11581 aagaattcga aacatcaaaa ggaagcgatt ttgctaaacc aacagatccg acatccttgc
    11641 tttataatgg tccattcttg ttgaaagggt tgactgcaaa atcttctgta gagtttgtaa
    11701 aaaatgagca atattgggat aaagaaaatg tccacctaga tactatcaat ctagcttact
    11761 atgatggatc agatcaggag tcgctagagc gtaacttcac tagtggagct tatagttatg
    11821 cccgtcttta ccctaccagc tccaactatt ctaaggttgc agaagaatac aaggacaata
    11881 tctattacac acaatcaggc tctgggattg ctggtctggg tgtgaatatt gatcgccaaa
    11941 gttacaacta tacttctaaa actacagatt cagagaaagt agctactaag aaggcattgc
    12001 ttaacaaaga tttccgtcaa gccttgaatt ttgctcttga tcgctcagct tactcagctc
    12061 aaatcaatgg taaagatgga gcagctttag cagttcgtaa tttatttgta aaaccagact
    12121 ttgtttcagc tggtgagaag acctttggtg atttagtcgc tgctcaactt cctgcttatg
    12181 gtgatgagtg gaaaggtgtg aatttagctg atgggcagga tggtttattc aatgctgaca
    12241 aggccaaggc agagtttgcg aaagctaaga aagctttaga agcagacggc gttcagtttc
    12301 ctattcatct ggacgttcca gtagaccaag catcaaaaaa ctacatatct cgtattcagt
    12361 cctttaaaca atctgtagaa acagttcttg gtgttgaaaa tgtcgttgtt gatattcaac
    12421 aaatgacaag tgatgaattc cttaatatta cttactatgc tgccaatgct tcatctgagg
    12481 attgggatgt atcgggagga gtttcatggg ggccagacta tcaagaccca tctacttacc
    12541 tggatatttt aaaaacaact agcagtgaaa ctacaaaaac atatttagga tttgataatc
    12601 caaatagccc ttcagtagtt caagttggtt tgaaagaata cgataaatta gttgatgaag
    12661 ctgccaaaga gacaagcgac ttgaatgtcc gttatgaaaa atatgcagcg gctcaagcat
    12721 ggttgacaga tagttcactc tttattcctg ctatggcttc ttctggtgca gcaccagtgc
    12781 tttcacgaat tgttccattt actggagctt ctgcgcaaac aggctctaag gggtcagatg
    12841 tttacttcaa atatttgaaa ttacaagata aagtggtgac taaggaagag tatgaaaaag
    12901 ctcgtgaaaa atggttgaaa gaaaaagctg aatcaaatga gaaagctcaa aaagaattgg
    12961 caagtcatgt gaagtaaata atttctaata gaactttctc tccatgagga gaaggttctt
    13021 ttgggatttt taaaggaaat aatatgaaaa aatatatttt tatgcgtgtt ttgcggtcat
    13081 tggtttcgat tttcttagta acgactttga cctacacgat tatctatacc ttggttcctc
    13141 gaaaattgat tttcaagcag gatcctaact ataataaaat tgcgacaacg gctgataaac
    13201 gtgataacta tgaaaatact gtgtttgagc gtatgggcta cattgagtat tacgatacta
    13261 aagagttgca agaaaaggca agtagcatgg attcttctgt aacagtagaa gcaaatgcga
    13321 ccaataaagc tatttatgaa aagtacatca atcaattagg tcatggttgg actttgggag
    13381 aatttactga aagtggtcaa ttctatgcta ctcgtgaaat tccaattttt gaacgtgttt
    13441 ttcacttcta tgctaacttg attgacattg accatacaaa taaaatccaa gaccctgaaa
    13501 atccagactt gaaacgctac cttcgttttg aaaatgatcc agctatcgga tggtcattgg
    13561 tcggttcagg aactaaacat aaatatctct tgtactttaa cagtcagttc ccatttgttc
    13621 atcaaaactt tgtgaacttg aatttaggtg actcttaccc aacctatgct aatacaccag
    13681 ttcttcaggt tattactcaa ggtcaaggac aaaccaaaac tgcccaagtt cagttcccaa
    13741 caggtaagaa aacgtcttct gtaaatattt actcaagaac ctacaagtca cctagtcagg
    13801 ctgactctcg tgaagtagct agctatggga aagatgatcc ttatacagcg actgaaagta
    13861 attaccaata tccatctatg attgtcagct ctgctattac tggtttgatt ggtttggttc
    13921 ttgcctatgc tcttgccgtg ccacttggtt cagccatggc tcgtttcaag aacacttgga
    13981 ttgatagcct ctcaacaggg gctttgacct tcttgcttgc tcttccaacg attgccttgg
    14041 tttacatcgt tcgattgatt ggatcatcta ttgcccttcc agattcattc cctatcttgg
    14101 gagctggaga ttggcgttct tacgttttac cagcagtcat ccttggtttg ttgggtgctc
    14161 ctggtacagc catttggatt cgtcgttaca tgattgactt gcaatctcaa gactttgttc
    14221 gtttcgctcg tgcaaaaggt ttgtctgaaa aagaaatttc aaacaaacac atctttaaaa
    14281 atgccatggt tccgctggtt tcaggaattc ctggtgccat cattggggtt atcggtggtg
    14341 caacccttac tgaaacagtc ttcgccttcc caggtatggg taaaatgttg attgactctg
    14401 taaaagcatc taataactct atggtcgttg gtcttgtctt catctttaca tgtatttcta
    14461 tcttctcacg tcttttggga gatatttgga tgactattat tgacccacgt attaaattga
    14521 ctgagaaagg aggcaaataa tgtctacaat cgataaagaa aaatttcagt ttgtaaaacg
    14581 tgacgatttt gcctctgaaa ctattgatgc gccagcatat tcttactgga aatcagtgtt
    14641 taaacaattt atgaagaaaa aatcaactgt agtcatgttg ggaatcttgg tagccatcat
    14701 tttgataagt ttcatctacc caatgttttc taagtttgat ttcaatgatg tcagcaaggt
    14761 aaacgacttt agtattcgtt atatcaagcc aaatgcggag cattggttcg gtactgacag
    14821 taacggtaaa tcgctctttg acggtgtctg gttcggagct cgtaactcca tcctcatttc
    14881 tgtgattgcg acagtgatta acttggttat cggtgttttt gtcggtggta tttggggtat
    14941 ttcaaaatca gttgaccgtg tcatgatgga agtttacaac gtcatctcaa acatcccacc
    15001 tcttttgatt gttattgtct tgacttactc aatcggagct ggattctgga atctgatttt
    15061 tgccatgagc gtaacaacat ggattggtat cgccttcatg atccgtgtgc aaatcttgcg
    15121 ctatcgtgac ttggaataca acttggcatc acgtactttg ggaacaccaa ccttgaagat
    15181 tgttgccaaa aatatcatgc ctcaattggt atctgttatt gtgacaacca tgactcaaat
    15241 gcttccaagc tttatctcat acgaagcctt cttgtctttc ttcggtcttg gattaccgat
    15301 tacagtgcca agtttgggtc gtttgatttc ggattattca caaaacgtaa caaccaatgc
    15361 ttacttgttc tggattccat tgacaaccct tgtcttggta tccttgtccc ttttcgtagt
    15421 tggtcaaaac ttagcggatg ctagtgatcc acgtacacat agataggagt agaaatgaca
    15481 aaagaaaaaa atgtaatttt gactgctcgc gatattgtcg tggaatttga cgttcgtgac
    15541 aaagtattga cagccattcg cggcgtttcc cttgaactcg ttgaaggaga agtattagcc
    15601 ttggtaggtg agtcaggatc aggtaaatct gttttgacaa agaccttcac aggtatgctc
    15661 gaagaaaatg gtcgcattgc ccaaggtagt attgactacc gtggtcagga tttgacagct
    15721 ttatcttctc acaaggattg ggaacaaatt cgtggtgcta agattgcgac tatcttccag
    15781 gacccaatga ctagtttgga ccccattaaa acaattggta gtcagattac agaagttatt
    15841 gtaaaacacc aaggaaaaac agctaaagaa gcgaaagaat tggccattga ctacatgaat
    15901 aaggttggca ttccagacgc agatagacgt tttaatgaat acccattcca atattctgga
    15961 ggaatgcgtc aacgtatcgt tattgctatt gcccttgcct gccgacctga tgtcttgatc
    16021 tgtgatgagc caacaactgc cttggatgta actattcaag ctcagattat tgatttgcta
    16081 aaatctttac aaaacgagta tcatttcaca acaatcttta ttacccacga ccttggtgtg
    16141 gtggcaagta ttgcggataa ggtagcggtt atgtatgcag gagaaatcgt tgagtatgga
    16201 acggttgagg aagtcttcta tgaccctcgc catccatata catggagtct cttgtctagc
    16261 ttgcctcagc ttgctgatga taaaggggat ctttactcaa tcccaggaac acctccgtca
    16321 ctttatactg acctgaaagg ggatgctttt gccttgcgtt ctgactacgc aatgcagatt
    16381 gacttcgaac aaaaagctcc tcaattctca gtatcagaga cacattgggc taaaacttgg
    16441 cttcttcatg aggatgctcc aaaagtagaa aaaccagctg tgattgcaaa tctccatgat
    16501 aagattcgtg aaaaaatggg atttgcccat ctggctgact aggaggaagg aaatgtctga
    16561 aaaattagta gaaatcaaag atttagaaat ttccttcggt gaaggaagta agaagtttgt
    16621 cgcggttaaa aatgctaact tctttatcaa caagggagaa actttctcgc ttgtaggtga
    16681 gtccggtagt gggaaaacaa ctattggtcg tgctatcatc ggtctaaatg atacaagtaa
    16741 tggagatatc atttttgatg gtcaaaagat taatggtaag aaatcgcgtg aacaagctgc
    16801 ggaattgatt cgtcgaatcc agatgatttt ccaagaccct gctgcaagtt tgaatgaacg
    16861 tgcgactgtt gattatatta tttctgaagg tctttacaat caccgtttat ttaaggatga
    16921 agaagaacgt aaagagaaag ttcaaaatat tatccgtgaa gtaggtcttc ttgctgagca
    16981 cttgactcgt taccctcatg aattctcagg cggtcaacgt caacgtatcg gtattgcccg
    17041 tgccttggtc atgcaaccag actttgttat tgcagatgag ccaatttcag ccttggacgt
    17101 ttctgtacgt gcccaagtct tgaacttgct caaaaaattc caaaaagagc tcggcctgac
    17161 ctatctcttc atcgcccatg acttgtcggt tgttcgcttt atttcagatc gtatcgcagt
    17221 tatttacaag ggtgttattg tagaggttgc agaaacagaa gaattgttta acaatccaat
    17281 tcacccatat actcaagcct tgctttcagc ggtaccaatc ccagatccaa tcttggaacg
    17341 taagaaggtc ttgaaggttt acgacccaag tcaacacgac tatgagactg ataagccatc
    17401 tatggtagaa atccgtccag gtcactatgt ttgggcgaac caagccgaat tggcacgtta
    17461 tcaaaaagga ctaaactaat aatggtttta taatttccat gtcaattttt atgggaatta
    17521 taaaactttt ttgttggact gatttgaaaa actttgaaag cttcctgaaa ga
//
LOCUS       DAVBGY010000043        17180 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVBGY010000043 DAVBGY010000000
VERSION     DAVBGY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17180
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.043"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(55..285)
                     /locus_tag="VQ620_001461"
     CDS             complement(55..285)
                     /locus_tag="VQ620_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764065.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(303..944)
                     /locus_tag="VQ620_001462"
     CDS             complement(303..944)
                     /locus_tag="VQ620_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2764066.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(956..1636)
                     /locus_tag="VQ620_001463"
     CDS             complement(956..1636)
                     /locus_tag="VQ620_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HET2764067.1"
                     /translation="MGMFVGMFKARVESHEIILDVKALMPWISAICLLIGFISMFLTF
                     NFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITGVVIPLAIFISLSEV
                     IILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFATPKDVLNYINSYDEG
                     ERQANLEQSFRILFQLHQYVLPALYIFLIILSFLTGEIQLLAFLLVGAIHVYINVMQL
                     PMVKRYFK"
     gene            complement(1692..1889)
                     /locus_tag="VQ620_001464"
     CDS             complement(1692..1889)
                     /locus_tag="VQ620_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764068.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(1889..2095)
                     /locus_tag="VQ620_001465"
     CDS             complement(1889..2095)
                     /locus_tag="VQ620_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2764069.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(2105..2419)
                     /locus_tag="VQ620_001466"
     CDS             complement(2105..2419)
                     /locus_tag="VQ620_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764070.1"
                     /translation="MWVLGFILFLIFFYSNDSKKIKKLEKKIKRLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(2638..2715)
                     /locus_tag="VQ620_001467"
                     /pseudo
     CDS             complement(2638..2715)
                     /locus_tag="VQ620_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(2712..4001)
                     /locus_tag="VQ620_001468"
     CDS             complement(2712..4001)
                     /locus_tag="VQ620_001468"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET2764071.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(4079..5326)
                     /locus_tag="VQ620_001469"
     CDS             complement(4079..5326)
                     /locus_tag="VQ620_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET2764072.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTFFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFVGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLDLSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFFGLLFTLSIPVGTACFSLVAVWNMQLT
                     WMLFVGFSLLAIFLTILNLKNDI"
     gene            complement(5517..6368)
                     /gene="rgg"
                     /locus_tag="VQ620_001470"
     CDS             complement(5517..6368)
                     /gene="rgg"
                     /locus_tag="VQ620_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HET2764073.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVDLTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKGNETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            6492..6833
                     /locus_tag="VQ620_001471"
     CDS             6492..6833
                     /locus_tag="VQ620_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET2764074.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITQISR
                     YGRIALGLPPR"
     gene            6891..6971
                     /locus_tag="VQ620_001472"
                     /pseudo
     CDS             6891..6971
                     /locus_tag="VQ620_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(7267..8970)
                     /gene="ilvD"
                     /locus_tag="VQ620_001473"
     CDS             complement(7267..8970)
                     /gene="ilvD"
                     /locus_tag="VQ620_001473"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET2764075.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(9184..10116)
                     /locus_tag="VQ620_001474"
     CDS             complement(9184..10116)
                     /locus_tag="VQ620_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HET2764076.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(10113..10970)
                     /locus_tag="VQ620_001475"
     CDS             complement(10113..10970)
                     /locus_tag="VQ620_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2764077.1"
                     /translation="MILSENREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(10974..12320)
                     /locus_tag="VQ620_001476"
     CDS             complement(10974..12320)
                     /locus_tag="VQ620_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2764078.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKSSAKEE"
     gene            complement(12333..12617)
                     /locus_tag="VQ620_001477"
     CDS             complement(12333..12617)
                     /locus_tag="VQ620_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2764079.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(12621..14651)
                     /locus_tag="VQ620_001478"
     CDS             complement(12621..14651)
                     /locus_tag="VQ620_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET2764080.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKVSSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYNDIKMIIKQED"
     gene            14842..15849
                     /locus_tag="VQ620_001479"
     CDS             14842..15849
                     /locus_tag="VQ620_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET2764081.1"
                     /translation="MTEKLINSKPNGVFALILIELTLVLGVFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNGNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            15852..16052
                     /locus_tag="VQ620_001480"
     CDS             15852..16052
                     /locus_tag="VQ620_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2764082.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            16204..16386
                     /gene="rpmF"
                     /locus_tag="VQ620_001481"
     CDS             16204..16386
                     /gene="rpmF"
                     /locus_tag="VQ620_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET2764083.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            16402..16551
                     /gene="rpmG"
                     /locus_tag="VQ620_001482"
     CDS             16402..16551
                     /gene="rpmG"
                     /locus_tag="VQ620_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2764084.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(17043..>17180)
                     /locus_tag="VQ620_001483"
     CDS             complement(17043..>17180)
                     /locus_tag="VQ620_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764085.1"
                     /translation="ESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWV
                     G"
BASE COUNT         5577 a         3612 c         2941 g         5050 t
ORIGIN      
        1 ctttgatttt cattgagtat aatacaaaaa tccgatgaac ttcaccggac tcttttattt
       61 tgaatttttg cctgctttac gcttttcagc gatttcggct gcctttcgag gcaagacaat
      121 ttccgttatg taagccgtcc caaaacgcag tacacctgca ataggagcaa agacaactgc
      181 tagatagtta tagaagaaat cgcctttgaa ggcataagct agcgctccaa tgatgaaaaa
      241 tagaacgact gcctgaatca ctgctaataa aattactcgt ttcatgtgac ctcctgactc
      301 tattatagca tgagaatcat caaaaagccg actaaattat tcaaagcgtg aagagaaata
      361 ctgtagacca gaccttttct gctaatgtaa gccaaaccca aactaaaacc aaggctaaaa
      421 tagacaaaaa attgttgcac atcacctgga aaatgaatca aggcaaatag aagactagat
      481 accagaagaa aaatcagggt tcgtttacta ttgtcctgct taggaaagag atagcgtgct
      541 aacatccctc taaaaacaat ctcttccgtc aaaggagcaa aaataaccac agcaaagaat
      601 gagaaaagtg gttgagacaa ggtcaagtct gtcgctattt gctgatttac tgaaggatca
      661 tctggcaaga agaattgaac gaccagagat aagaaccaaa ccaaggcagg aagccaaata
      721 aatcggttaa agccgctctt ctcaatataa acaggagctt tctgatacca tttgtaaatg
      781 ccgtacacat atactccagc caagaccaca tagagtagag taacagcata gggtgaagcg
      841 cctaaagcaa gcgacgcagt cgcgagcccc tgaataaagc catagataaa gaaaaaggat
      901 agaagggcta gaagaaacca gccaaggttt ttaagtaatt tcatagataa ctcctttatt
      961 tgaaataacg ttttaccata ggtaactgca tcacattgat ataaacatgg atggctccta
     1021 caagcaagaa agctagtaac tgaatctctc ctgtcaagaa agaaaggata ataagaaaaa
     1081 tatataaggc tggtaagaca tattggtgta attggaataa aattcgaaaa ctctgttcca
     1141 aattagcctg acgctcccct tcatcataag aatttatata gttcaagaca tcctttggtg
     1201 tagcgaaaaa ttccaaatca aactgacgaa caatcgcaat ggttttaaaa agagattttt
     1261 gagcgactaa gaataccaca aagagtaaga aagaaaggaa aaatgtttga gggtttgtat
     1321 gcaatataat cacctcactt aatgaaataa aaatagccaa tggaatcact acacctgtaa
     1381 tattaaaagc aatggttcca aactcaagat tccgatacat ttgcacataa taggtttcat
     1441 tcagatcgtc atccatttcc tcttgataca aggaatgaaa ttttctgctt ttctttaaga
     1501 aattgaaagt caaaaacata ctaatgaaac ctatcagtaa acaaatagct gatatccatg
     1561 gcatcaaggc ttttacatct aaaataattt cgtgggattc gacacgtgcc ttaaacatcc
     1621 ctacaaacat gcccaagaac cccccaagac aatagacatc aaaaataaca atctacgttt
     1681 ctttttcata ttcattctcc tttttcactt gctagatttt tggatttctt ttcaatccat
     1741 tcaattactg ggatgagagc aaagtagacc caaacaaatt ggtcgctttg atagggatta
     1801 aaccagctta ggtccatccc aatcagtaga aatacgctga ctaataaagc tatgaccact
     1861 acataataaa tcactttata cttgttcatc actcgtcctc ctccaaacga aataccgatt
     1921 cgactgtttc gttgaaaatt tgagatattt tcagggcaat gataatggat ggggtgtact
     1981 catcccgttc tagtaggcta atggtctgtc tggaaacccc tgccagtttg gctaggtcgg
     2041 tttgattgag accatcgcga gctcgaagct cttttagacg attttttagt tgcatgttac
     2101 acacctactc tccgtcaaat tcaacggttt ggatatcctc aatacgttgc aacttgaatt
     2161 tttcttttcc cgtattatct acacgtcgta gctttaccca ttcctcatca acatccacaa
     2221 cttcccagtt atctggccca atatacactc ccgttataat tggttccttt ccaatcattt
     2281 cttgtaataa tctcgacatt tctgcatttc ctttctcttt tcgctcaagt cttttgattt
     2341 tcttctctag tttcttgatt tttttagaat cattagaata aaagaaaatc agaaatagta
     2401 taaaccctag tacccacatc atagctcctt tcttcttttc tcttaacttg cttacattgt
     2461 aacacatctt tttctttttg acaagtatag ttgtcaaaaa gtttatgatt tttgtcattt
     2521 tgcaaaagaa aaaggtcagg agtaagttcc tgaccacttt atctatcatt aatactcttc
     2581 taaaatctct tcaaaccacg tcagcttcac cttgccgtag gtatggttac tgacttcgtc
     2641 agttctatcc acaacctcaa aacagtgttt tgagcaacct gcggctagct tcctagtttg
     2701 ctctttgatt ttcattgagt ataaaatcct agtttttcaa agatttctga gaagttttgg
     2761 ctgattgtct caagtgacac ttgcacttct tctcgggttt ggttgttctt gaccgtcact
     2821 tgtccgcttt cgacttcgct ctctcctagg gtgatgaggg tcttagccgc aaagacatct
     2881 gctgacttga actgagcttt gagcttacgg ttgaggtaat cacgctctgc tttgaaacct
     2941 tgttggcgaa gggcttggac tagttctagg gctttgacat ttgctccatc acccaagact
     3001 gcgatataga catctagggc attttcgata gggagggcca cgccttgttt ttcaaggatg
     3061 agaagcagac gctctacacc aagcccaaaa ccaaatccag cagtttcagg gcctccaaag
     3121 taagcaacca aaccatcgta gcgaccaccc gcacagactg tcaagtcatt gccctcaatc
     3181 tctgtgataa actcgaaaat ggtgtggttg tagtagtcca gaccacgcac catattggta
     3241 tcgatgatgt aatctactcc aagattttcc aacatctgac gcacagcatc aaaatgagtt
     3301 tggctttctt catcaagaaa gtccaagata gacggcgcat tctctactgc caccttgtct
     3361 tctttttcct tagagtccaa gacacgaaga ggattttcct ccaagcgacg ttggctatcc
     3421 ttagacaagg tctccttgag cggtgtcaaa tagtcaatca aggcttggcg gtaggctgca
     3481 cggctctcag gatttccaag agtgttgagg tgcaatttga caccttgaat accgatttcc
     3541 ttcaaaaaat gggctgccat agcgattgtt tccacatcgg tagctggatt gctagagcca
     3601 aaacattcaa caccaatctg gtggaattgg cgcaagcgcc ctgcctgtgg acgctcataa
     3661 cggaacatag gtcccatata gtagaacttg cttggctttt gcacttctgg ggcgaagagt
     3721 ttattttcca cataggaacg gacaacgggc gcagttcctt ctggacggag ggtaatatga
     3781 cggtcaccct tgtcataaaa atcgtacatt tccttggtta cgatatccgt tgtatctccg
     3841 acagagcgac tgataacctc gtaatgctca aaaataggcg tgcgcacttc tgcatagtta
     3901 tagcgcttga aaatctcacg ggcaaagccc tcaacgtact gccacttagc agattcagca
     3961 ggtaaaatat cctgcgttcc ttttggtttt tgtaatttca tagggaatcc tctttaaact
     4021 taatagtctt attttaccac aaataaaggg attaaaacag taagaaaaaa attaggattt
     4081 agatatcatt tttgagatta agaattgtca aaaaaatagc tagcaaggaa aaaccaacaa
     4141 atagcatcca agtcaactgc atgttccata cggctactag tgaaaaacaa gctgttccca
     4201 caggtatgga taaggtaaac aatagaccga aaaaattact agtacgagct agaacctctg
     4261 gagctagatt tttcatgagc atggcactaa tctttggttg aactttacca gacacataca
     4321 gagtaaagaa gagaaatagc aaaccaagca cgacttgatt gaataaatta gccaaaccaa
     4381 ctagactaag tcctacggtc gcccacatca tcaatctagg caaggactgc ttcccaaaat
     4441 aatcattgcc cgtaaggcta ctgatgatga ttgctactaa aacacagaat tgattgataa
     4501 atagtgcctc tgtataagaa aaattcaaga gagaatggct caaaaagaag atattataaa
     4561 ttccacccaa agcgccaccc aaggaattaa taagcaagac agcaaagagc ataaaaccaa
     4621 agtttttctg tccactttta agaaaaacga gacgtaaatt tcggtaaatt gttaggaact
     4681 ggtctttgat agaaagcttc tcatttttta agttttcacc atcagcagat gacattgaca
     4741 ggctcaattt gctttttcct aaaaagagga tagtggctga tactaggaaa aagcaggcat
     4801 tgattcccgc aacgagagaa aaattgttga ccgatagatc taagagccag actccgaaag
     4861 cttgaccacc aatagctgaa atataggtga tgaactgtga aaaagaataa gcctccatca
     4921 gatcatcttc agctactttt tccttaataa gaggcatacg caggccacct acaaaatcac
     4981 tgatgatatc actaatgaca ttgatcaaac acaggctaga aaaggcaaag agactagctt
     5041 gctgaacaac tagggctgct agaaaaaata gaaccgcctg aaacaaaccg ctatagacca
     5101 tccatttgac cttgtccctc gtgtaatctg cccgaatccc tgcaaaaact gtaaagaagg
     5161 tcggaagaat catgacaata ttcgccatag caacagcaaa agatgcttgt gacaaggtcg
     5221 atgcatagac gataaagacc aggttgaaaa tcgaagcacc aaaagcattg aagaagcgtg
     5281 ataaagtcaa aattctataa gctggatttc taaacaatag tttcatagat aatctccctt
     5341 cttgttctaa gttctacatc aagaatagct ttagtatagc acttatgaat ttgcgaaacg
     5401 attcttttga atatatgcaa caaacaaaat gagcaaaaaa acgatagaac ttttgtaatg
     5461 acctgagaga gggcaacagt tctatcgctt caaatttttt cttggtttgc agatactcaa
     5521 cattcggtcg tatttctata gtattcggca gatttattac agccaagcat ctcaaaaata
     5581 cggacagcat cctccatctt tttctgacct tccttgactc taccttgctt gctatcaagg
     5641 agaccttctg cccacagata aacaattcgg aaataggtct catttccctt atagaaatgc
     5701 tcttcgataa cacgtttaaa ataataggca ttggtaaatt cttcacactc aatactagct
     5761 aaaaagccat tcaatagtat agtatgaaaa aggtttcgat tgccagacat ttccattaga
     5821 aaatcggatt tacgtaccat ttctcgtaca tatctagtaa aaagagaaac agataaaaat
     5881 ggagaactga ctgaaaataa attgagttca tagattcccc agatctcggt agaaaacaaa
     5941 taatcatgaa ggacttttcc ttcctctgct gttaagtcta ccctttcatc tatgctcttc
     6001 atataagact tgataataat ggcatttaga atatgtttct gtttgttgtg agaatgggca
     6061 tgcttttgat actccctgcg atataagtcc tcaagaggtg ctatattctt tggttccaag
     6121 acatctgtaa tttcttttct caactcagaa tctgtatcat actggaaacc tctcgccaga
     6181 aagaggatct cctccacact ggcagatata ttttccagag caaatagaaa cttttccacc
     6241 gaaagctcac tctgacctgt ttcaaaacgg gacaacatag acggcgaaaa ttgtcctccg
     6301 gttgcttgtc tcagtgagat atttcttgat tctcgtaatt gtctaaagac ttttccaatc
     6361 tgctccatag acttcccctt gattccgtat tttcttcatt ttatcatatt tttcagaaaa
     6421 ttcatcaaaa acttgccaaa ttgtcagaat tatgagaaaa tagaggatat ttatcacgtg
     6481 gagggactgc tatgagagac gatatcaaaa tcaatgaccg tgctttggcc ttgcaagacc
     6541 aaattatcga aaaactagag aaagtttttg atacagatgt ggaattggat gtttacaatc
     6601 taggtctgat ttatgaaatc aatctggatg aaacggggct ctgcaagatt gtcatgacct
     6661 tcaccgatac tgcctgtgat tgcgccgaaa gcctgcctat tgaaattgtg gcaggtctga
     6721 aacaaatcga gggtatcaaa gatatcaagg ttgaagttac ctggtcgcct gcttggaaaa
     6781 tcacacaaat cagtcgctat ggccgtattg cccttggact accacctcgt taagcaggcc
     6841 aatcactttt aaagatgaaa aaagcaagcc gaaaatggct tgctttttga tactcaatga
     6901 aaatcaaagg gcaaactaga aaactagccg caggttgctc aaaacactgt tttgaagttg
     6961 tggatagaac tgacgaagtc agctcaaaac actgttttga ggttatggat agaactgacg
     7021 aagtcagccc aaaacactgt tttgaggttg tggatagaac tgacgaagtc agcccaaaac
     7081 actgttttga ggttgtagat agaactgacg aagtcagctc aaaacactgt tttgaagtta
     7141 tggatagaac tgacgaagtc agctcaaaac actgttttga ggttgtagat agaactgacg
     7201 aagtcagtaa ccatacctac ggcaaggcga cgttgacgtg atttgaagag attttcgagt
     7261 atgagtttat tttttacctg acttgtccat attccagaag tctgtcacgg ctccgcgtga
     7321 agcagatgat acgatgtggg catatttacc gaggacacca cggctgtaaa gtggtggcaa
     7381 ggttgtttct gccttgcgtt tttcaagttc ttcttcggat acggccatgg aaatttcttt
     7441 ggtatcttgg tcaaccgtaa cgatatcgcc ggtacggaga taggcaattg gtccaccatc
     7501 ctgagcttca ggagcgatgt gtccaacaac cagaccataa gtaccaccag agaaacgtcc
     7561 gtccgtcaag agggccacct tatctccctg acctttacca acaatcattg atgaaagtga
     7621 cagcatctca ggcataccag gaccaccttt aggtccaaca aaacgaacaa cgactacatc
     7681 gccatcaacg atttcatctg tcagaacggc ctgaatcgca tcttcttctg agtcaaagac
     7741 cttagctggc ccaacgtgac gacgcacttt aacacctgat accttggcaa ctgcaccgtc
     7801 aggagcaagg ttcccgttca agatgataag cggaccatcc gcacgttttg gattttcaag
     7861 tggcatgata actttttggc ctggagtcaa gtctgcaaag tcagccaagt tttcagctac
     7921 agtcttacca gtacatgtga tgcgatctcc gtgaaggaaa ccatttgcca acaagtactt
     7981 cataaccgca gggacaccac cgacttcgta gaggtcttgg aagacatact gaccagatgg
     8041 tttcaagtcg gccaagtgag gcacacgttc ttgaatcgta ttgaagtcct caagtgacaa
     8101 gtcaacattt gcggcatggg caatggcgag caagtgaaga gtggcgtttg tagaaccacc
     8161 gagagccatc gttacagtaa tagcatcttc aaaggcttca cgagtcaaga tatctgatgg
     8221 tttgagacca agttccaaca tcttaacaac agcacgtcct gctgcttcga tatcttcttt
     8281 cttatcagct gattcagctg ggtgagagga tgaccctggc aaactcatcc ctagaacttc
     8341 gatagcagtt gccatggtat tagcagtata cataccacca caaccaccag ggccagggca
     8401 ggcattacat tcaagacgtt tcacgtcctc agctgtcatg tcaccgtggt tccattttcc
     8461 gataccttca aagacagaaa ccaagtcgat atctttacca tcaagatttc ccggtgcaat
     8521 agttccacca taggcgaaaa tagctgggat atccatatta gcaatagcaa tcatagatcc
     8581 aggcatgttc ttgtcacagc caccgatagc gacgaaggca tccacgttgt gaccactcat
     8641 agccgcctcg atggagtccg cgatgatgtc acgagatgtt agagagaaac gcataccagg
     8701 cgttcccata gcgatcccgt ctgctacggt aatggttcca aactgtacag gccaagcgcc
     8761 tgcagatttg acaccttctt tagccagttt cccgaaatca tgcaagtgaa tgttacatgg
     8821 tgtattttcc gcccaagtcg aaatcactcc cacaatcgat gtttcaaagt ccttatctgt
     8881 cataccagtc gcacgaagca tagcacggtt aggtgattta accatgctgt cataaatgct
     8941 actgcggtga cgtttatcta attcagtcat cttatccctc ccatttcagt ttttactatt
     9001 atagcacaat ttaaaggcaa tgaacagaag aaaattctat acgcatgtga aatatttaaa
     9061 attaaaaaca gcaaagcgga ttagtgcact ttctgatgac cagaatatgc tttttaatcc
     9121 gctttcttta aataacgtaa tgtaattttt acagaaattc tttcagataa gtgtatttaa
     9181 catctatctt gcattataaa tttctagaac cttctctttt atattcgatt cactcaaacc
     9241 atactcatta agaagataat ccattttccc tacttgacca aatctttctt gaacacccat
     9301 ccgatgaatt tttgttattc catcatcaga gaataattca cataaagcac tgccaattcc
     9361 acctatctga ttgtggtttt ctacagtaaa tatagttttt ccacttaaca ttgtttttat
     9421 ctgttctggt atcggtttga ttctaaataa atctatcaca cctactgaat aacctaattt
     9481 agacagttca tctgcaactc gaatacttgg agcaaccatt ataccagaag caacgattac
     9541 aagatcttcc ccatgtctta actcaatgta gcccttagaa aaatcttctc caccttgata
     9601 cacaggaact ggagcttttc taattgttcg aatatatttt agtcctttta agtctaatgt
     9661 ctggttcaat atttcacgaa attggatatc atcagttgct tcaaaaatga tcgatttagg
     9721 aattaaacgt aacaatccaa tttcttcaaa tggcatatgt gtcccaccat tcatttctgc
     9781 agttactcct gcatctgatc caatcacagt ggcatccaat tgtgcgtatc caagagaaat
     9841 aaataattga tcaaatactc ttcgtgaagc aaaaggacca aatgtatgaa gataaggtct
     9901 aaacccctga atagacaagc ctgctgcaag gccgaccatt tctgcttcca taatcccaac
     9961 attcacataa cggtctccaa agtccttttc aagattatga gtagccatcg aacttgacaa
    10021 atcggcttct aagactacta tatcagaata actttgatta gcctctaaaa ggaagtctct
    10081 atatacatgc cgtaattctt tcgtacttct catcattctg tttcctccaa ttcctgactt
    10141 aatctttcta caactgaagt taacatttgt ttctcctcta cagtagggcg aagatgatga
    10201 ttggatttca tttcttccag ctcttgaacc ccttgacctt taatagtatc taatacaatg
    10261 cacttaggtg atgaattatt tgactgtttt aattggacaa tcccttcata aatttctcta
    10321 atatctgaac ccttgaccct aatggattca aatccaaatg ctgaaaattt ttctacgaaa
    10381 tcacctggat tacaaatatc ctttgtaaaa ccatctaatt gttttttgtt atcatcaaca
    10441 aatacaatta agttggataa ctgttgatga gaagcaaact gtatagcctc ccaacattgt
    10501 ccttcattta actcaccatc tccaacaata gcataagtat aaaagggact ctttcttatt
    10561 ctctgaccat atgcaagtcc agttgcaaca ctaattcctt gtcctaaaga gcccgttgtc
    10621 atatctatgc ctggcgttag atttctatca ggatgagacg gtaatttggt tccatttgta
    10681 tttaaagaat ataagaattc tttgtcaaag aaaccattca aatagagtgt actgtataga
    10741 gctggtcctc catgaccttt tgataatatg aaataatctc tatctcgtgc tgcaaatatt
    10801 tctggagtca ttggcattat ttcaccataa agcactgcta aaacttctac gatagacaga
    10861 cttcctccgt aatgtccgaa tccaagatga ttcaatgttc taagagtatt taatcggatg
    10921 ttagtcgcaa attttcttaa ctcatcttct ctattttcac ttaaaatcat cccttactcc
    10981 tcctttgcag atgacttttt aataaaggat actccaaaca taactgctag aataagaaca
    11041 agaccaatca caatgcctgc ttgtgaacca aattgattta acattcctaa aataattcct
    11101 gatagaccaa aatctgcatc tgagaaagtt gatccttgga aaccaagtcc tcccaaaact
    11161 ggcattaaaa agactggaag aaaactgatt aaaatacctt gtaaaaatgc tccaatagtg
    11221 gctccacgaa caccaccaga tgcattccca atgacacctg cagtcgctcc acagaagaaa
    11281 tgaggcacaa cgcctggtaa gataacaacc gttcctgaag caatcataat tgccatactt
    11341 actaaaccac caataaaact agagataaat ccaattagaa ctgcattggg tgcataagta
    11401 taaacaatcg gacaatccaa agcaggtttt gaattaggta caagacgctc tgaaatacct
    11461 ttaaaggctg gaacaatttc acccaaaata aggcgaacac ctgctaaaat aacaaatacc
    11521 cctgctgcaa attgacctgc taattgtaaa gcataaacta gaccacttgt accactactg
    11581 atttcttttt ctatatattc tgaccctgca aagatagcta caataatgta aataactgcc
    11641 atggataaag taatactaac agtgctatca cgtaaaaaag ctaaactctt tggaaattta
    11701 atgtcctctg ttgattttga tttgtcaccg ataaggctac cagtaaaacc actcaaccaa
    11761 tatcccaaag aactgaaatg acctaaagcc accttgtcat ttccagttaa ttgaaccata
    11821 tatttttgca caaatgctgg ggaaatactc ataataatac cgagtgctaa tcctcctagt
    11881 aagataagag gcaagctagt aaagccagca actgataaaa tgaccgcaat catacatgcc
    11941 atatatagag tgtggtgccc tgttaaaaaa atatatttaa atcgagtaaa acgagcgatt
    12001 aagatattga acaccatgcc tgcaaacata atcattgcag tagctgagcc atatgttgtt
    12061 aaagctacag ctacaattgc ttcattattc ggcacaacgc cagataaatg aaaagcatgc
    12121 tcaaacatgg taccaaatgg atttaaagaa ttttgtacaa ttcctgcacc accagataca
    12181 actaagaaac caacaaaggt cttaattcca cctttaataa tatcaggtaa tttcttcttc
    12241 tgaagaacta atcctaaaat tgcaattaaa gctactaaaa tagctggtgt actaacaata
    12301 tccaatatga acttcatcat gacgctagcc tcctatataa gtcctttttc ttcacaaagt
    12361 ttagtaatta attctcgtag ttcatccata tcaataatac tatttaagat acgaacatct
    12421 ccaagatgac tagctgaatc agctagatca cgaccaacaa tccaaatatc agctgcattt
    12481 ggatctgctc cacctaaatc ataatgttca acttctacat ctgaaacatt caaatcactc
    12541 aatacagatt caatattcat ctgtaccata aaacttgaac ctaatcctga accacaagct
    12601 gtaccaattt ttaacattat ctaatcctcc tgtttaatta tcattttaat atcattatag
    12661 ttttttgatg atattaaagt ttgaacatga tttttatctc ttaaaattgt tgttaaatgt
    12721 gacaaagcct ttaaatgact ctcattatca atggctgcaa tacaaatcaa caatcttacc
    12781 tcttgttctg gattatccaa taaataaatc ggttcttcca aaactaacat tgacattcct
    12841 atttcattca caccttcatc tggccgagcg tgaggaattg ctactccctt ccctaaatta
    12901 ataaaaggtc caaactcttc tactttttga atcattgcct cagggtagtt ctcagttatc
    12961 ttatcttgat ccaaaagcgg tttagctgct aaacgaatcg cctccttcca tcctaatttt
    13021 tgcgaactaa cctgataggt ttctttggta ataagttgtt ctagcactgg tacaatttcc
    13081 tttctatcat ttttttgata aagataattc tttaacgcca atcttaattc caattcttgt
    13141 gtaataattc catatctttt gacaatattc aggatttgtt caatctcaaa atctccatac
    13201 tctaaattcg gaaaatcttt taacactagt tctactagtt gtattgcttg ctcttcagtc
    13261 atcataaccg aaactagata atttggcttt tctgtctcca cctttatggt agaaaaaacc
    13321 atatcatagt cactactaac tttcacctgt aaatcatcaa tctttgaggt tcctataaac
    13381 tcaatttgag gaaataatgc taatagattc tcttttaaca tcaatgaaga actaacacca
    13441 ttaggacaaa tgattgctgc tttataccat ttttgaggca aagtatctgc tttctttaaa
    13501 taacctccga aatggataac aaaatatgct gtttcactat caggtatggg attgtcaata
    13561 gcgtccatca agggcatcaa agaatctttg actaattcaa ataaatcagg ataatgttct
    13621 ttaacatgca atacatattc atttgaacta ggtaggccga actttaatct atagtaagcc
    13681 ggtataaggt ggcggcgaag attttctctc aatccttccc tttgtttaaa atgtaacaaa
    13741 gaaatatctt ccattctact tataatagcc tctgttaatt gattaaagta aaccggagca
    13801 acatctactt caccttcaaa gcaacttgat aataaaacgg tgatatagcg ataatcatcc
    13861 tcagaaaaca ccgtatctat aattcccaaa tcaaccactg tatccaataa aatagtcgtt
    13921 atatcttgaa taacaggaga tactaatgtc tctgaaagac atactctttc aacatccctt
    13981 tgatacctac acagaatgaa tactaaaccg aaaaggtaaa cttttaattg attaacaata
    14041 ggtactagct gtagcttctc ataataatct ttaactacct gatcaatcaa atcataagtt
    14101 aatgaatacc cccaactgga taaaacataa tccaaacccc aaatccctat ggaggattcc
    14161 agcaactcac taaccatttg aaaagctaag cggtgcttat tccactctga accgtgtaaa
    14221 gtataacctt ttgctctact gtaccctagc tccaaatcat tatctaacat aatctttctt
    14281 aatgattgaa tatcagataa ggttgtattc ttacttactt tcaaaaagtc ttggtaatga
    14341 ttattcgata taaaatctaa tcggcaaaaa gtgtaaagat agattaaagc taagcgagtc
    14401 gactttggta aaaccaattc atccgactta ataatatctg tcaaagactg cttcgtacga
    14461 tttgataaac tatagcgacc ttgtttttta tccagcacta tccctttatt agctagataa
    14521 ggcactaaat aatctattcc ttctttgact tcctttatag gtaagctcac cttaacagat
    14581 aattcatata atgatagctc acaatgatcc atcaaagtca tcaaaataac tagtgctcta
    14641 taatcaaaca tttctcttcc tttctaacta caattatagt gtaaaaatta taagaataaa
    14701 agagtatttg agcacaatat aatctcacct tttaaaagaa attattgggc tgaaactttt
    14761 tagtgattat tttcaacttt ctattgactt ttaaaaacaa agtgatatca tttagatatc
    14821 aaataatggg aggtcattct tatgactgaa aaactaatca attcaaaacc aaatggtgta
    14881 ttcgcattga ttctcattga attgacgctc gtacttggtg tctttatatt tataatgggt
    14941 gttggttcgg aaaatatttt tggaattatt atcggacctt tactaatcgt aattgcaggt
    15001 ctagctcatg ctggtttaaa agttgtcaaa cctcaggaag ctctggttct gacactcttt
    15061 ggtaattata caggtaccat caaagaacct ggtttttact ttgtcaatcc cttcagcgta
    15121 gcagtcaacc ctgcaaacca cactcgactt ggacaaagtg gtgatgttag cacaaaatct
    15181 ccttttttag gagctaaatc atcaaatggc aatgatgtaa atcttgaaat tggcaagaaa
    15241 cacatttccc tcaaagtcat gaccttgagc aattctcgtc aaaaaatcaa tgattgctta
    15301 ggaaaccctg tagaaatcgg tatcgcggta acttggagag ttgtcgatac cgctaaggca
    15361 gtcttcaacg ttgataacta caaagaatat ctttcattgc aatgtgatag cgccctccgt
    15421 aatattgtcc gtatctaccc ttacgatgtg tctcctaatg tggatactac gggtgacggg
    15481 caagcagatg aaggtagtct ccgtggctct agcgaaattg ttgctaaccg tattcgtgaa
    15541 gaaatccaaa gtcgtgttga ggatgctggc ttggaaatcc ttgaagcacg tatcacttac
    15601 ctagcttatg ctccagaaat tgctgccgtt atgcttcaac gccaacaagc atctgccatt
    15661 attgatgcac gtaagatgat tgtagatggt gctgtaggaa tggttgaaat ggcactagaa
    15721 cggctcaatg aaggggaatt ggtagaactt gacgaagaac gaaaagctgc catggtttca
    15781 aatctccttg tcgttctttg tggcaatcat gatgcacaac caattgtcaa cacaggaagt
    15841 ctttactaaa gatggctgaa gctaaaaaaa agcagatccc ccttcgactc tcaacaaagt
    15901 tatacgctgc actcgcatca tgggcagaag acgactttcg ttcagtcaat gggcaaatag
    15961 aatatctcct cacagagtgt gtcaagcaac ggaaaaaaga cggaaaatac gtatcagaaa
    16021 ccattgacga accatttgag attgatattt aacaccttct cctgtctgcc gtgtcggata
    16081 ggagaatttt ttcatctttt tttgtcaagt aactttactt tacaaaaaaa atgtgttatc
    16141 ctagtatggt tgatgaaaat cagtagattg aatcgaatat aaatacctaa aggagaaatc
    16201 aaaatggcag tacctgcacg tcgcacttca aaagcgaaga aaaacaaacg tcgtacacac
    16261 tacaaagtaa cagctccatc tgtaaacttt gacgaaacta ctggagatta ctcacgttct
    16321 caccgtgtat cacttaaagg atactacaaa ggacgtaaaa tcgctaaagc tgcatcagct
    16381 gaataataga agggagatac catgcgcgta aatattacac ttgaacacaa agaatctggt
    16441 gaacgcttgt accttacttc taaaaacaaa cgtaacactc cagaccgcct tcaattgaag
    16501 aaatactcac caaaacttcg caaacacgtt gtgtttacag aagttaagta agcattcaat
    16561 acgaatcaat acagaagaaa aacgcttgat cagcgttttt tctttttgtt aatttcacta
    16621 tattgcaagg gaaatcattc gaatgactac ctttttcact accttttttg aaataagaat
    16681 acggattcgg acgattaaaa gtaagagaaa ctcaaatgta agttggggtt ccttttttgt
    16741 atatttttca aataacgcct ctacactatt tgtagcaaat tcaccaacta cagttgtatc
    16801 ttagttaaaa taagttagaa tatgtaagtg agtaccagat ataccaagac atcgtcacca
    16861 tctaaggtat attcaaaata caaaagttga ccaactagat ttctgaatat ccttatatat
    16921 ccattcttaa aattggttta aatagcgtag tcttttaaac tagttttgag aatccaaaaa
    16981 atcttcctac atatgtaaga agatttttta gttcagaatg attagattta gctaatggat
    17041 acctatccta cccactcacc gttagcattg actcgatagc catcgggtgt agtcgtgttt
    17101 actaataaat ctcctgaatt ataggtatag taccatttac cagaaactgt aaaccaacca
    17161 gtagccattg aacctgattc
//
LOCUS       DAVBGY010000044        16131 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVBGY010000044 DAVBGY010000000
VERSION     DAVBGY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16131
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.044"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            145..6831
                     /locus_tag="VQ620_001484"
     CDS             145..6831
                     /locus_tag="VQ620_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET2764086.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKLEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVLTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVARVRTLIGKEPKMPKTVGFVYSDGSREKLPVTWSQ
                     VDVSQAGVVTVKGTANGREVEAHVEVLAIAKELPTVKRIAPNTDLNFVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRIEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQIRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLRFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGKERIFT
                     AINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSEASQTN
                     KAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(7079..7934)
                     /locus_tag="VQ620_001485"
                     /pseudo
     CDS             complement(7079..7934)
                     /locus_tag="VQ620_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8145..8504)
                     /locus_tag="VQ620_001486"
     CDS             complement(8145..8504)
                     /locus_tag="VQ620_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HET2764087.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            8596..9552
                     /locus_tag="VQ620_001487"
                     /pseudo
     CDS             8596..9552
                     /locus_tag="VQ620_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            9536..10093
                     /locus_tag="VQ620_001488"
     CDS             9536..10093
                     /locus_tag="VQ620_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2764088.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQADLTNAQLILQGHETLDQFVIKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            10086..10340
                     /locus_tag="VQ620_001489"
     CDS             10086..10340
                     /locus_tag="VQ620_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764089.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERSVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            10352..12406
                     /locus_tag="VQ620_001490"
     CDS             10352..12406
                     /locus_tag="VQ620_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET2764090.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            12585..13445
                     /locus_tag="VQ620_001491"
     CDS             12585..13445
                     /locus_tag="VQ620_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET2764091.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            13656..14366
                     /gene="ccdA2"
                     /locus_tag="VQ620_001492"
     CDS             13656..14366
                     /gene="ccdA2"
                     /locus_tag="VQ620_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET2764092.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            14386..14952
                     /locus_tag="VQ620_001493"
     CDS             14386..14952
                     /locus_tag="VQ620_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HET2764093.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            14963..16075
                     /gene="msrB"
                     /locus_tag="VQ620_001494"
     CDS             14963..16075
                     /gene="msrB"
                     /locus_tag="VQ620_001494"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET2764094.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
BASE COUNT         4898 a         3050 c         3568 g         4615 t
ORIGIN      
        1 catatctacg gcaaggcgac actgacgaag tttgaattgg attttcgcag agtgtaaatt
       61 gttgaaacta ggttgtcata ccatgtatac cacttgggct ttggtagtag ctaactgcac
      121 taaatataat ataaggaggg ttttatgggg aaaggccatt ggaatcggaa aagagtttat
      181 agcattcgta agtttgctgt gggagcttgc tcagtaatga ttgggacttg tgcagttcta
      241 ttaggaggaa atatagctgg agaatctgta gtttatgcgg atgaaacact tattactcat
      301 actgctgaga aacctaaaga ggaaaaaatg atagtagaag aaaaggctga taaagctttg
      361 gaaactaaaa atgtagttga aaggacagaa caaagtgaac ctagttcaac tgaggctatt
      421 gcatctgaga agaaagaaga tgaagccgta actccaaaag aggaaaaagt gtctgctaaa
      481 ctggaagaaa aagctccaag gatagaatca caagcttcaa gtcaagaaaa accgctcaag
      541 gaagatgcta aagctgtaac aaatgaagaa gtgaatcaaa tgattgaaga caggaaagtg
      601 gattttaatc aaaattggta ctttaaactc aatgcaaatt ctaaggaagc cattaaacct
      661 gatgcagacg tatctacgtg gaaaaaatta gatttaccgt atgactggag tatctttaac
      721 gatttcgatc atgaatctcc tgcacaaaat gaaggtggac agctcaacgg tggggaagct
      781 tggtatcgca agactttcaa actagatgaa aaagacctca agaaaaatgt tcgccttact
      841 tttgatggcg tctacatgga ttctcaagtt tatgtcaatg gtcagttagt ggggcattat
      901 ccaaatggtt ataaccagtt ctcatacgat atcaccaaat accttcataa agatggtcgt
      961 gagaatgtga ttgctgtcca tgcagtcaac aaacagccaa gtagccgttg gtattcagga
     1021 agtggtatct atcgtgatgt gactttacaa gtgacagata aggtgcatgt tgagaaaaat
     1081 gggacaacta ttttaacacc aaaacttgaa gaacaacaac atggcaaggt tgaaactcat
     1141 gtgaccagca aaatcgtcaa tacggacgac aaagaccatg aacttgtagc cgaatatcaa
     1201 attgttgaac gaggtggtca tgctgtaaca ggcttagttc gtacagcgag tcgtacctta
     1261 aaagcacatg aatcaacaag cctagatgcg attttagaag ttgaaagacc aaaactctgg
     1321 accgttttaa atgacaaacc tgccttgtac gaattgatta cgcgtgttta ccgtgacggt
     1381 caattggttg atgctaagaa ggatttgttt ggttaccgtt actatcactg gactccaaat
     1441 gaaggtttct ctttgaatgg tgaacgtatt aaattccatg gagtatcctt gcaccacgac
     1501 catggggcgc ttggagcaga agaaaactat aaagcagaat atcgccgtct caaacaaatg
     1561 aaggagatgg gagttaactc catccgtaca acccacaacc ctgctagtga gcaaaccttg
     1621 caaatcgcag cagaactagg tttactcgtt caggaagagg cctttgatac ttggtatggt
     1681 ggcaagaaac cttatgacta tggacgtttc tttgaaaaag atgccactca cccagaagct
     1741 cgaaaaggtg aaaaatggtc tgattttgac ctacgtacca tggtcgaaag aggcaaaaat
     1801 aaccctgcta tcttcatgtg gtcaattggt aatgaaatag gtgaagctaa tggtgatgcc
     1861 cactctttag caactgttaa acgtttggtc aaggttatca aggatgttga taagactcgc
     1921 tatgttacca tgggagcaga taaattccgt ttcggtaatg gtagcggagg gcatgagaaa
     1981 attgctgatg aactcgatgc tgttggattt aactattctg aagataatta caaagccctt
     2041 agagctaagc atccaaaatg gttgatttat ggatcagaaa catcttcagc tacccgtaca
     2101 cgtggaagtt actatcgccc tgaacgtgaa ttgaaacata gcaatggacc tgagcgtaat
     2161 tatgaacagt ctgactatgg gaatgatcgt gttggttggg ggaaaacagc aaccgcttca
     2221 tggacttttg accgtgacaa cgctggctat gctggacagt ttatctggac aggtacggac
     2281 tatattggtg aacctacacc atggcacaac caaaatcaaa ctcctgttaa gagctcttac
     2341 tttggtatcg tagatacagc cggcattcca aaacatgact tctacctcta ccaaagccaa
     2401 tgggtttctg ttaagaagaa accgatggta caccttcttc ctcactggaa ctgggaaaac
     2461 aaagaattag catccaaagt agctgactca gaaggtaaga ttccagttcg tgcttattcg
     2521 aatgcttcta gtgtagaatt gttcttgaat ggaaaatctc ttggtcttaa gactttcaat
     2581 aaaaaacaaa ccagcgatgg gcggacttac caagaaggtg caaatgctaa tgaactttat
     2641 cttgaatgga aagttgccta tcaaccaggt accttggaag caattgctcg tgatgaatct
     2701 ggcaaggaaa ttgctcgaga taagattacg actgctggta agccagcggc agttcgtctt
     2761 attaaggaag accatgcgat tgcagcagat ggaaaagact tgacttacat ctactatgaa
     2821 attgttgaca gccaggggaa tgtggttcta actgctaata atctggttcg cttccaattg
     2881 catggccaag gtcaactggt cggtgtagat aacggagaac aagccagccg tgaacgctat
     2941 aaggcgcaag cagatggttc ttggattcgt aaagcattta atggtaaagg tgttgccatt
     3001 gtcaaatcaa ctgaacaagc agggaaattc accctgactg cccactctga tctcttgaaa
     3061 tcgaaccaag tcactgtctt tactggtaaa aaagaaggac aagagaagac tgttttgggg
     3121 acagaagtag caagagttcg tacattaatt ggtaaagaac caaaaatgcc gaaaacagta
     3181 ggttttgtat acagcgatgg tagtcgtgaa aaacttccag taacttggtc tcaagtcgat
     3241 gttagtcaag caggagttgt gactgttaaa ggtacagcaa atggacgtga ggtagaggct
     3301 catgtcgagg ttctagcaat tgcgaaagaa ttaccaacag ttaaacgtat tgctccaaat
     3361 actgacttga attttgtaga caaatctgtt tcttatgttt tgactgatgg aagtgtacaa
     3421 gagtacgaag tagatagctg ggagattacg gaagtagata aagccaaact ttcagtagct
     3481 ggatcacgta ttcaaatgac tggtcagtta gctggagaaa ctattcatgc aacccttgtg
     3541 gtagaagaag gaaatgctgc agcacctgta gtgccaactg ttactgttgg aggtgaggca
     3601 gtaacaggtc ttactagtcg acaaccaatg caatatcgta ctctatctta tggtgcccaa
     3661 ctgccagaag tcacagcaag tgctgaaaat gctgatgtga cagttcttca agcaagcgca
     3721 gcaaacggca tgcgtgcgag catctttatt cagcctaaag atggtggtcc tcttcaaacc
     3781 tatgcaattc aattcctaga agaagcacca aaaattgctc acttgagttt gcaagtggaa
     3841 aaagctgaca gtctcaaaga agaccaaact gtcaaattgt cggttcgagc tcactatcaa
     3901 gatggaacgc aagctgtatt accagctgac aaagtaacct tctctacaag tggtgaaggg
     3961 gaagtcgcaa ttcgtaaagg aatgcttgag ttgcataagc caggagcagt cactctcaaa
     4021 gcggaatatg agggagctaa aggccaagtt gaactcacta tccaagccaa tactgagaag
     4081 aagattgcgc aatctatccg tcctgtaaat gtagtcacag atttgcatca aaaacctact
     4141 cttccaacaa cagtaacggt tgagtatgac aaaggtttcc ctaaagctca caaagtcact
     4201 tggcaagcta ttccgaaaga aaaactagac tcctatcaaa catttgaagt actaggtaaa
     4261 gttgaaggaa ttgaccttga agcgcgtgca aaagtctctg tagaaggtat cgtttcagtt
     4321 gaagaagtca gtgtgacaac tccaatcgca gaagcaccac aattaccaga aagcgttcgg
     4381 acatatgatt caaatggtca cgtttcatca gctaaggttg catgggatgc gattcgtata
     4441 gagcaatacg ctaaggaagg tgtctttaca gttaatggtc gcttagaagg tactcaatta
     4501 acaactaaac ttcatgttcg cgtatctgct caaactgagc aaggtgcaaa catttctgac
     4561 caatggaccg gttcagaatt gccacttgcc tttgcttcag attcaaatcc aagcgaccca
     4621 gtttcaaatg ttaatgacaa gctcatttcc tacaataacc aaccagccaa tcgttggaca
     4681 aactggaatc gtactaatcc agaagcttca gtcggtgttc tgtttggaga ttcaggtatc
     4741 ttgagcaaac gctccgttga taatctaagt gtcggattcc acgaagacca tggagttggt
     4801 gcaccgaagt cttatgtgat tgagtattat gttggtaaga ctgtcccaac agctcctaaa
     4861 aaccctagtt ttgttggtaa tgaggaccat gtctttaatg attctgccaa ctggaaacca
     4921 gttactaatc taaaagcccc tgctcaactc aaggctggag aaatgaacca ctttagcttt
     4981 gataaagttg aaacctatgc tgttcgtatt cgcatggtta aagcagataa caagcgtgga
     5041 acgtctatca cagaggtaca aatctttgcg aaacaagttg cggcagccaa acaaggacaa
     5101 ataagaatcc aagttgacgg caaagactta gcaaacttca accctgattt gacagactac
     5161 taccttgagt ctgtagatgg aaaagttccg gcagtcacag caagtgttag caacaatggt
     5221 ctcgctaccg tcgttccaag cgttcgtgaa ggtgagccag ttcgtgtcat cgcgaaagct
     5281 gaaaatggcg acatcttagg agaataccgt ctgcacttca ctaaggataa gaacttactt
     5341 tctcataaac cagttgctgc ggttaaacaa gctcgcttgc tacaagtagg tcaagcactt
     5401 gaattgccga ctaaggttcc agtttacttc acaggtaaag acggctacga aacaaaagac
     5461 ctgacagttg aatgggaaga agttccagca gaaaatctga caaaagcagg tcaatttacc
     5521 gttcgaggcc atgtccttgg tagtgatctt gttgctgagg tcactgtacg agtgacagac
     5581 aaactaggcg aagctctttc agataaccct aactatgatg aaaacagtaa ccaggccttt
     5641 gcttcagcaa ccaatgatat tgacaaaaac tctcatgacc gcgttgacta tctcaatgac
     5701 ggagatcatt cagaaaatcg tcgttggaca aactggtctc caacaccatc ttctaatcca
     5761 gaagtatcag cgggtgtgat cttccgtgaa aatggtaaga ttgtagaacg gactgttgcc
     5821 caagccaaac ttcgtttctt tgcagatagt ggtacggatg caccaactaa actcgtttta
     5881 gaacgctatg tcggtccaga gtttgaagtg ccaacctact attcaaacta ccaagcctac
     5941 gacgcagacc atccattcaa caatccagaa aattgggaag ctgtgcctta tcgtgcggat
     6001 aaagacatcg cagctggtga tgaaatcaac gtaacattta aagctgtcaa agccaaagct
     6061 atgagatggc gtatggagcg taaagctgac aagagcggtg ttgcgatgat tgagatgacc
     6121 ttccttgcac cgagtgaatt gcctcaagaa agtacgcaat cgaagattct tgtagatgga
     6181 aaagaacttg ctgatttcgc tgaaaatcgt caagactatc aaattaccta taaaggtcaa
     6241 cggccaaaag tctcagttga agaaaacaat caagtagctt caactgtggt agatagtgga
     6301 gaagatagcc ttccagtact tgttcgcctc gtttcagaaa gtggaaaaca agtcaaggaa
     6361 taccgtatcc agttgactaa ggaaaaacca gtttctgctg tacaagaaga tcttccaaaa
     6421 ctcgaatttg ttgaaaaaga tttggcctac aagacagttg agaaaaaaga ttcaacactg
     6481 tatctaggtg aaactcgtgt agaacaagaa ggaaaaactg gtaaagaacg tatctttaca
     6541 gcgattaatc ctgatggaag taaggaagaa aaactccgtg aagtggtaga agctccgaca
     6601 gaccgcatag tcttggttgg aaccaaacca gtagctcaag aagctaaaaa accacaagtg
     6661 tcagaaaaag cagatacaaa accaattgat tcaagtgaag ctagtcaaac taataaagcc
     6721 cagttaccaa atacaggtag tgcggcaagc caagcagcag tagcagcagg tttagctctt
     6781 ctaggtttga gtgcaggatt agtagttact aaaggtaaaa aagaagacta gaaattttga
     6841 taccttcttt atcattaaag accaaagaaa aactcttgtc ctaaatagaa gataaagagt
     6901 ctggaacaaa atagttctca accacaaaaa agctagatat ttccaattgc ggaactctag
     6961 ctttttaatt ttgagtcgtt ctcttattgt atagtagatt gaaactagaa tagtacactt
     7021 ctacttctaa aacattgtta gaaatcaatt tgactgtcct tatcgatttg tcctgttctt
     7081 atttcatttc actatgtttt aggaggttat tggccataag taccaatccc atgtcaattc
     7141 tcacttgacg cttccctctc agattacatc tcttgtaacc caaacaagcc tttatctgcc
     7201 caaagacagg ttccacatca atcttgcgtt gagcgaaaat ctgtctactt ggggagataa
     7261 aagcgcctga cattctttag ctttcaagtt ttgatagcgt tcgttcatat acagtccctt
     7321 ttgagggact gattcaggtt catcagcgta gtaaaccttg atttcctgtt gaaagtctgt
     7381 ctgtgttttc tggtacttga tatggtgaaa acgatagcac cagccatcag gatgtgtgta
     7441 gctatcctcc ttgtcattat agtgccaatt cgctaagttt ttagctgact gtttataccc
     7501 tctcttttgt tccttatcaa acatggcata tttaatcaga aggggagtga tttcttcttg
     7561 aaaatagacc tgtatctgtt cttgaagttt ggcggagaat ttctctgtcg ttttcttcca
     7621 cacaaaacta tacttgttgg cattggcttc aatcttagtt ccgtcaataa acaaacaatc
     7681 taaggtcact aactcttcca tttttcaatt tttcgaccag agaaaatccc ttgtgaatag
     7741 gcaaatagaa gagtagatac aagcatttta gggtgataag acgggcgatc aaaggcatga
     7801 tagaaggcgt ggaagtgaca atcctccaag gtattcacca ctttttcaat agtaaaaacg
     7861 agatgatctt gtggcaagaa ggaactgatt tctagtggta aagttgtttg atttgtgtta
     7921 tagtgaatat gcatgggata gcctttctaa atggtttatt aagcaattct attttaccaa
     7981 gactatcgca accatgcaaa aaagcaacgg caaatccgtt gccttttttg gagataagag
     8041 actattgtcc cagactcttt tctttgtata aaattgtata gtgttattgt ctatactatt
     8101 ttgtagagaa agcaaaaatc cctagaaaat gcttagggat tcgactattt cattaagaac
     8161 aattcaacaa tcatttctat atacatgata agggttaata agaatcctgc acgccagaag
     8221 aatttgataa atttaggata gtaaaagctg cgttttttca aaaggaagaa aaagacgaga
     8281 ataatggcta ggatcgagag ggctagcccc agtctaggga ggaaattatg ggtaaaggtt
     8341 ttagctgtaa tcaagtaata ctcaaatacc aagactggaa aagccaaatc cgcaaagttt
     8401 cgtcctagtt tttttaatct aaaaagtttg gttaggataa tgcagacgac taaggtcagt
     8461 atcaataata aaatagatgc taatttcatt aaaatcatac ccatattgta tcataaaaaa
     8521 aggctaatgg aaacgagaaa cagaggaatt tataggaaag tcaggctttt gagattgtgg
     8581 gtgatttttg ttataatgaa agttataaaa tcttataata ccttgaatga ttattatcga
     8641 aaactctttg gagaaaagac ttttaaagtc cctattgatg cgggatttga ctgtcccaat
     8701 cgtgatggaa ctgtggctca tggaggctgt actttttgta cggtttccgg ttctggagat
     8761 gccattgtgg caccagatgc gcctatccgt gagcaatttt ataaggaaat tgactttatt
     8821 catcgtaagt ggccagatgt tcagaagtat ctggtttatt ttcaaaattt taccaacacc
     8881 catgaaaagg tggaagttat tcgagagcgt tatgaacagg ctatcaatga gccaggtgtg
     8941 gtaggaatca atattggaac gcgcccagac tgtttaccag acgaaaccat cgaatatttg
     9001 gctgagttat cggagtgcat gcatgtgacg gttgaattgg gcttgcaaac tacttatgaa
     9061 gcaacctctg acctgattaa ccgtgcgcat tcttatgaat tgtaggtgga aacggtcaag
     9121 cgtttgagaa agtatccaaa aattgagatt gtttcccatt tgatcaatgg tttgcctggt
     9181 gaaacccatg agatgatggt tgaaaatgtc cgccgttgtg tcacggataa cgatattcaa
     9241 gggattaaac tgcatttgct ccatcttatg acaaatactc gtatgcaacg agattaccat
     9301 gaggggcgtt tacaattgat gagtcaggac gaatatgtca gggtcatctg tgaccaactg
     9361 gaaattattc ccaaacatat cgtcatccat cgaattacag gagatgcgcc tagggatatg
     9421 ctgcttgggc ctatgtggag cctcaataaa tgggaagctc tcaacagcat tgagatggag
     9481 atgcgacgtc gtggaagtgt tcaaggatgc aaggctgtaa aacaggagtt tgaaaatgaa
     9541 aagaccactt gagatggcac atgatttttt ggctgaggtc gtgacaaaag aggatgtcgt
     9601 agtggatgcg actatgggaa atggtcatga cacgcttttt ttagccaagc tagccaagca
     9661 agtctatgcc tttgatattc agaagcaagc cttggaaaag acccaagagc gtttgcatca
     9721 ggctgacttg acaaatgccc agttaatctt gcaaggccat gagacactgg accagtttgt
     9781 gataaaagct aaggcaggga tttttaatct gggctatttg ccgtcagctg ataagtctgt
     9841 catcacccga ccgcagacaa cgattgaggc attagaaaag ctatgtggct tacttgtcaa
     9901 aggtggacga attgctatta tgatttacta tggtcatgaa ggaggcgatc tcgagagaga
     9961 tgctgtcttg gattttgtga tccagttgaa ccaacaagag tacacagctg ccatttaccg
    10021 aactttaaac caagtcaaca acccgccgtt tttagtgatg attgaaaaat tagagagata
    10081 cagacatgga taaacaatac ctacacgaaa agctggatgc catgcgccag aattttgttg
    10141 aatcaaccca ccacgaacga tcggtgggcg tgctagacca agcgcatatg agcaaaaaaa
    10201 tgcttaaaat caagaaaaaa ttagttgctc ttgaaatgga acggtgccag agaaaaattg
    10261 agcacaaaga ctgttccaag attgaccaaa aaatcaaaga gcagaaggag atatttgaat
    10321 cctgttgtaa aaaagattaa ggaggacgtg cgtggaatta ctgatttacc tcatcctatt
    10381 tttactggtt ttgattgtct caagtacaac caataagctc ctgccctttt tgcctctccc
    10441 tttggtgcag attcttttgg gaattgtgat tggtctcttt ttacccaata ctgactttca
    10501 ccttaatacg gagttgtttt tggcactggt tatcggaccc ttgcttttcc gagaggctga
    10561 agaagcagat gttacggcta ttttaaaaca ctggcgaatc attgtttatc tcatatttcc
    10621 agtgattttt atctcgacct tgagtttggg tggcttggcc catcttcttt ggttcagcct
    10681 tcccttggca gcttgcttgg ctgttggggc agcccttggt cctacggact tggtggcctt
    10741 tgcctctctt tcggagcgtt ttagctttcc taagcgcgtg tccaatattc ttaagggcga
    10801 aggactcttg aatgatgctt ctggtttggt ggcttttcag gtagctttga cagcttggac
    10861 aactggagct ttttctctgg ggcaagctag cagttcgctc atcttttcaa tcctaggcgg
    10921 ttttttaatt ggatttttaa cggccatgac caaccgcttc ctccatacct tcttgctaag
    10981 tgtgcgcgca acggatattg ccagtgaact tttattagaa ttgagtttgc ctctagtgac
    11041 cttctttctg gcagaagaag ttcatgtttc aggtattatt gccgtcgtag ttgctggaat
    11101 tttaaaggca agtcgcttca agaaaatcac gctcctcgaa gcccaagtgg atacggtgac
    11161 cgagacggtc tggcatacag tgacctttat gctcaacggt tctgtctttg tgattttagg
    11221 gatggagttg gaaatgatag cagaacctat cttgaccaat ccaatctata atcctctact
    11281 tttattgcta tctctcatcg cccttacctt tgtcctcttt gtcattcgtt ttattatgat
    11341 ctatggctat tatgcctata gaacccgacg cctcaagaaa aagctaaata agtatatgaa
    11401 ggacatgttt ctcttgacct tttcaggtgt taagggaacg gtgtcgattg ctacgattct
    11461 cttgatacca agtaatctag aacaggagta tcctcttttg cttttccttg ttgcaggtgt
    11521 gacgcttgtc agctttttaa caggtctctt ggtcttgcct catctttctg atgaagagga
    11581 agaaagcaag gattatctca tgcatatcgc cattttgaat gaagtaacgc tagagttgga
    11641 aaaagagttg gaagacacca gaaataaact tcccctctat gcggctattg acaattatca
    11701 tggacgtatt gaaaatctca ttttaagcca agaaaaccag gatgatcaag aagattgggc
    11761 tgctttgaaa ctcttgattc ttagtattga aagtgatggt ttggaacagg cctatgaaga
    11821 ggggaacatt agcaatcgtg cttaccgagt ttaccaacgt tatctgaaaa atatagaaca
    11881 aggaatcaat cgtaaacttg cctcaagact gacctattat tttcttgttt ccttgaggat
    11941 tttacgtttt ctccttcatg aagtttttac tcttggaaag accttccgta gctggaagga
    12001 caaggagcaa agccgtctcc gtgctcttga ttatgaccaa attgcagagc tctatcttgc
    12061 caatacagag atgattattg aaagtttgga aaacctgaag ggagtctaca gacgctcttt
    12121 gattagtttt atgcaggagt ctcgtcttcg agaaacagct attatcagca gtggtgcctt
    12181 tgtcgaacgg gttatcaatc gtgtcaaacc caacaatatc gatgaaatgc tgagaggcta
    12241 ttatctggag cgcaagttga ttttcgaata cgaagaaaaa cgattgatta cgactaagta
    12301 tgccaagaaa ttacgacaaa atgtaaataa cttagagaac tattccttga aggaagctgc
    12361 caataccctg ccgtatgata tggtggaatt ggtaagaaga aattagttaa tactcttcga
    12421 aaatctcttc aaaccacgtc agcgtcgcct tggattatat atgtgactga cttcgtcagt
    12481 ttcatctaca acctcaaagc agggctttga gcaacctgcg gctagcttcc tagtttgctc
    12541 tttgattttc attgagtata agattataaa tgaaggagta tgacatgaaa aagtggtgga
    12601 aagagctgat agacaagcct ttattaaaag cttttttgca ttattatcaa gcatcagata
    12661 gtgagttgac cagtgtcgca gtagcctact attggttgat ttcgattttc cccctgcttt
    12721 tggtggtggt caatatcctc ccctattttc agattcctgt gggagaattt ctgtgcttta
    12781 tgaaagacgt tctgccccca agcctctatg aaggtgtgga aaaaatagcc agagaagttt
    12841 tgacgcaacc ttcaacaggt ttattgagtt tttctgtttt atcggcgtta tggagttttt
    12901 caaaatctat gaatttcttg caaaaagctt ttaacaaggc ttatggcgtc gaaaagagtc
    12961 gtggacttat ttcccatcag atgctaagtc tcctagtcag ttttggcttg cagctcctct
    13021 ttgcgtttgc cttgtttttg atcttatttg gtcagatgat tttggcttta cttgctcatt
    13081 attggacaaa agatggcatt atctatcagg ctttacaagg gttggcaggt cctctgattt
    13141 acgccctcct ctttgctatc ttggtcatgc tttattattt tcttcccaat ctatctaaca
    13201 ggaaaattag ctatacccta ccaggcagtg cctttgttct cttagtgatt ttaggtttac
    13261 taactctgtt ctctagttat ctcaattact atgtccatca tttggtggat gtccgaattt
    13321 taggttccgt tcttcttgtt gttatgatgt tttggtttat cttgattgct aaaattgtta
    13381 tcctaggcgc ggttatcaat gccagtatgc agagtttgaa agatccggtc tttaaaaaag
    13441 actaactatt tatccattac aaaagcgcat agtatcaaaa ctttatagaa ccttgatact
    13501 atgcgctttt gtgctttgac agttttttgt atagtttttg tccaagattt ctaaatcatc
    13561 tgattaaatt cctattttcc cctatcattg tccctgtttt ttcgaaaatg gaaggagtat
    13621 aataaatcta tcaataaaat gataggagga actttatggg tcatattttc ttttttctaa
    13681 gtgtcttttt ggcagggatt ctatccttct tttctccttg tatcttacct ttgttaccgg
    13741 tctatacagg agtgttacta gatgataagg atggtgctca ggcttctagc ggcaaatttt
    13801 caatctcagt tactagttta ttacgaactc tggcctttat agcaggaatt tcctttatat
    13861 ttattttgtt gggctatgga gctggttttt taggcgattt gctttatgct tcttggttcc
    13921 aatatcttac tggggcaatt attatccttc ttggtttgca ccaaatggag attctacact
    13981 ttaaggggct ttataaggaa aagaggctac aactgcaagg acaggggcaa aatggtaagg
    14041 gctatagtca ggcattttta ttgggcttga cctttagttt tgcttggacg ccttgcgtgg
    14101 ggccggttct ggggtctgtt ttggccttgg cggcttcagg tggttcagga gcttggcagg
    14161 gagctggtct catgttggtg tatacgctgg gcttggcgct accattcttg cttctagctc
    14221 tgacctctag ttatgttttg aaacatttcc gaaaacttca tccctatctc ggaatcctca
    14281 aaaaagtggg tggttttctc attattgtga tgggactctt ggttctgttt ggaaatgctt
    14341 caattttaag tcaattattt gaataaaatg gaaaggaata tcaatatgaa aaaatggcaa
    14401 acatgtgttc ttggagcagg ttcgctcctt tgtttgacgg cttgttcagg caagtccgtg
    14461 actagtgaac accaaacgaa agatgaaatg aagacggagc agacagctag taaaacaagc
    14521 gcagctaaag ggaaagaggt ggctgatttt gaattgatgg gagtagatgg caagacctac
    14581 cgtttatctg attacaaggg caagaaagtc tatctcaaat tctgggcttc ttggtgttcc
    14641 atctgtctgg ctagtcttcc agatacggat gagattgcta aagaagctgg tgatgactat
    14701 gtggtcttga cagtagtgtc accaggacat aagggagagc aatctgaagc ggactttaag
    14761 aattggtata agggattgga ttataaaaat ctcccagtcc tagttgaccc atcaggcaaa
    14821 cttttggaaa cttatggtgt ccgttcttac ccaacccaag cctttataga caaagaaggc
    14881 aagctggtca aaacacatcc aggattcatg gaaaaagatg caattttgca aactttgaag
    14941 gaattagcct aggaggtgtc ttatgaatga taagttaaaa atcttcttgt tgctaggagt
    15001 attttttcta gccataaccg gtttctatgt tctattgata cgaaatgcag ggcagacaga
    15061 tgcctcgcaa attgaaaagg cggcagttag ccaaggagga aaagcagtga aaaaaacaga
    15121 aattagtaaa gacgcagact tgcacgaaat ttatctagct ggaggttgtt tctggggagt
    15181 ggaggaatat ttctcacgcg ttcccggggt gacggatgcc gtttcaggct atgcaaatgg
    15241 tagaggagaa acaaccaagt acgaattgat taaccaaaca ggtcatgcag aaaccgtcca
    15301 tgtcacctat gatgccaagc aaatttctct caaggaaatc ctgcttcact atttccgcat
    15361 tatcaatcca accagcaaaa ataaacaagg aaatgatgtg gggacccagt accgtactgg
    15421 tgtttattac acagatgaca aggatttgga agtgattaac caagtctttg atgaggtggc
    15481 taagaaatac gatcaacctc tagcagttga aaaggaaaac ttgaagaatt ttgtggtggc
    15541 tgaggattac catcaagact atctcaagaa aaatccaaat ggctactgcc atatcaatgt
    15601 taatcaggcg gcctatcctg tcattgatgc cagcaaatat ccaaaaccaa gtgatgagga
    15661 attgaaaaag accctgtcac ctgaggagta tgcagttacc caggaaaatc aaacagaacg
    15721 agctttctca aaccgttact gggataaatt tgaatccggt atctatgtgg atatagcaac
    15781 tggggaacct ctcttttcat caaaagacaa atttgagtct ggttgtggct ggcctagttt
    15841 tacccaaccc atcagtccag atgttgtcac ctacaaggaa gataagtcct acaatatgac
    15901 gcgtatggaa gtgcggagcc gagtaggaga ttctcacctt gggcatgtct ttacggatgg
    15961 tccacaggac aagggcggct tacgttactg tatcaatagc ctctctatcc gctttattcc
    16021 caaagaccaa atggaagaaa aaggctacgc ttatttacta gattatgttg attaatactc
    16081 ttcgaaaatc tcttcaaacc acgtcagcgt cgccttaccg tatgtagggt t
//
LOCUS       DAVBGY010000045        15613 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVBGY010000045 DAVBGY010000000
VERSION     DAVBGY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15613
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.045"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(1..741)
                     /locus_tag="VQ620_001495"
                     /pseudo
     CDS             complement(1..741)
                     /locus_tag="VQ620_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567576.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(879..2501)
                     /gene="groL"
                     /locus_tag="VQ620_001496"
     CDS             complement(879..2501)
                     /gene="groL"
                     /locus_tag="VQ620_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET2764095.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2517..2801)
                     /gene="groES"
                     /locus_tag="VQ620_001497"
     CDS             complement(2517..2801)
                     /gene="groES"
                     /locus_tag="VQ620_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET2764096.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(3071..3466)
                     /locus_tag="VQ620_001498"
     CDS             complement(3071..3466)
                     /locus_tag="VQ620_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET2764097.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3544..4305)
                     /locus_tag="VQ620_001499"
     CDS             complement(3544..4305)
                     /locus_tag="VQ620_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET2764098.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(4338..4964)
                     /locus_tag="VQ620_001500"
     CDS             complement(4338..4964)
                     /locus_tag="VQ620_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HET2764099.1"
                     /translation="MIFTYNKEHVSDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(4980..5297)
                     /locus_tag="VQ620_001501"
     CDS             complement(4980..5297)
                     /locus_tag="VQ620_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET2764100.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(5294..5593)
                     /locus_tag="VQ620_001502"
     CDS             complement(5294..5593)
                     /locus_tag="VQ620_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HET2764101.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(5878..6069)
                     /locus_tag="VQ620_001503"
     CDS             complement(5878..6069)
                     /locus_tag="VQ620_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HET2764102.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFSFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(6230..6904)
                     /locus_tag="VQ620_001504"
     CDS             complement(6230..6904)
                     /locus_tag="VQ620_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764103.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYAK"
     gene            complement(6910..7356)
                     /locus_tag="VQ620_001505"
     CDS             complement(6910..7356)
                     /locus_tag="VQ620_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2764104.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(7470..7973)
                     /locus_tag="VQ620_001506"
     CDS             complement(7470..7973)
                     /locus_tag="VQ620_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET2764105.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7970..8332)
                     /locus_tag="VQ620_001507"
     CDS             complement(7970..8332)
                     /locus_tag="VQ620_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HET2764106.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(8398..10257)
                     /locus_tag="VQ620_001508"
                     /pseudo
     CDS             complement(8398..10257)
                     /locus_tag="VQ620_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(10274..10388)
                     /locus_tag="VQ620_001509"
                     /pseudo
     CDS             complement(10274..10388)
                     /locus_tag="VQ620_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
     gene            complement(10515..10745)
                     /locus_tag="VQ620_001510"
     CDS             complement(10515..10745)
                     /locus_tag="VQ620_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2764107.1"
                     /translation="MDKPMLVFKRFGHQTYLMVQKEAKRCGIEFMGGPQGQVVRFWDN
                     REKNQDLVLIKDIEQELKITKSVASNLVKRIV"
     gene            complement(10869..11585)
                     /locus_tag="VQ620_001511"
     CDS             complement(10869..11585)
                     /locus_tag="VQ620_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2764108.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHVIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(12466..13881)
                     /gene="ply"
                     /locus_tag="VQ620_001512"
     CDS             complement(12466..13881)
                     /gene="ply"
                     /locus_tag="VQ620_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HET2764109.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13892..14224)
                     /locus_tag="VQ620_001513"
     CDS             complement(13892..14224)
                     /locus_tag="VQ620_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764110.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(14295..14903)
                     /locus_tag="VQ620_001514"
     CDS             complement(14295..14903)
                     /locus_tag="VQ620_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764111.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14916..15374)
                     /locus_tag="VQ620_001515"
     CDS             complement(14916..15374)
                     /locus_tag="VQ620_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HET2764112.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            15499..>15613
                     /locus_tag="VQ620_001516"
     CDS             15499..>15613
                     /locus_tag="VQ620_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2764113.1"
                     /translation="MNYEASKQLTDTRFKLLVGVQRTTFKEMLAGLKTAYQK"
BASE COUNT         4502 a         3601 c         2857 g         4653 t
ORIGIN      
        1 ttgatatgag aatagtcttt ctcaacatcc ttatccaaca agcatgctgt ggcgtccttc
       61 ttaacttgaa ccaggtcaat attctgggct tgggctgcca tagacgcagc cacaataaca
      121 ttgacgatag atatcatact cctcacacat ctctactgaa cgtttgtagc cttgattttt
      181 cttgaaatcg ttgggaagat agtgggtcgt gtaaattttt tgcacatcga ttccgatgct
      241 attgatggtt tgagaattct tatgaggact gatggtcaag gctgaaccaa agtagtcaaa
      301 gcccaagtcc atagccactt gcgctgtttt atccagtcgg tagtcaaaac aaaccttgca
      361 acggtcgcca ccttcgggct cctcctctag tcccctaact agttttcggt attaattggg
      421 ttcgtaggga gcttctaggt actgaaccgt atttcctgtc cgatcattaa aatcactaac
      481 aaatttcttg atgacataga cccgcttatg gtattctact ttgggatgga tattagaatt
      541 ggcaaaatag atggtcacat ctgcatactt ggtcaaatat tctagtgtat aggtactaca
      601 aggggcacag caaacatgca cgagaatggt tggccgctgc tcatttttct cccatgcttg
      661 taccattttc tgcatgacac ggtcataatt aatcttctga ttggggggca tcttgcacag
      721 aatttcttct acatcgatca tgttcttctc ctttttctag tcttatttta tcatataagt
      781 tagaagagct caaatcaatt tagaagtgaa tattataaaa agaagggtta gtcattccct
      841 ccttttgtgt tttttataag ttgttttcta tagaaagctt acatcatccc gcccatcata
      901 cttggatcca ttgctggagc tggggctact ggttctggtt tattggctac gactgcttct
      961 gttgtcaaaa tcaagctggc tacagatgct gcattttgta gggctgaacg actcacttta
     1021 actggatcaa tgataccttg atcaatcatg ttaacccact cgccagttgc tgcgttgaat
     1081 cctataccaa gctcagcatt tttcaaacga tcgataacga tagatccttc aaatcctgca
     1141 ttgtgagcaa tttgacgaac gggttcttcc aaagcacgga gaacaatatt acgtcctgtt
     1201 gcttcatctc ctgtcaattc caaggtagca acagctggaa tcacattggc aagagctgtt
     1261 ccaccacctg caacaatacc ttcttcaaca gctgcacgag tagcgttgag ggcatcttca
     1321 atgcggagtt tcatttcttt caactcagtt tcagttgcgg ctccgacctt aataaccgct
     1381 acaccacctg acaatttggc caagcgttct tgcaattttt cacggtcaaa ttcagaagtt
     1441 gtagtttcga tttgagactt gataaccgca acacggtgag aaatcgcttc aggatttcct
     1501 gcaccttcta caataaccgt gctatctttg tccacggtca ctctcgctgc ttgaccaaga
     1561 gcttcaattg tcgcatcttt caactcaaga ccaaggtctt ctgtgataac tgttccgcct
     1621 gttaagatgg cgatatcttc aagcatggct ttgcgacggt caccaaaacc aggtgccttg
     1681 actgctacta cgttgaaggt tccacgaatc ttgttcaaaa caagagttgg aagagcctcg
     1741 ccatccacat catccgcaat aatcaagagt ggacgattgc tttggagaat gctttccaaa
     1801 agtggcaaga tttcttggat attggaaatt ttcttgtctg taatcaaaat gtacggattt
     1861 tcaaggtcag ccaccatttt ttcgctatca gtcaccatgt actgtgaaag gtaaccacgg
     1921 tcaaactgca ttccttctac gacttcaagc tctgtttcca taccacgtga ctcttcgatg
     1981 gtgatgacac cgtctttgcc aactttttcc attgcttcag agatgtactc accaactttt
     2041 tcagaacgag aagatacggc tgcaacttga gcgatagctt ctttattggc aacagggatg
     2101 gcgttgtttt tcaaagcttc aactgctgcg gcaactgctg tttcaatccc acgacgaata
     2161 ccgattggat ttgcacctgc tgtgacgttt ttgattcctt cacggacgat tgcttgggtc
     2221 aaaacagttg cagttgtagt tccatcacct gcgatatcat tggtttttga agccacttct
     2281 gataccaatt tggcacccat attttcaaaa tggtcttcta attcaatttc tttggcaata
     2341 gtcacaccgt cattggtaat caatggtgag ccaaatgatt tttcaagaac gacattgcga
     2401 ccttttggtc ccaaggttac tttaacagtg tctgcaagga tatcgacacc acggaccata
     2461 gctgaacggg catctgatga aaatttaatt tcttttgaca tacttacttt ctccttctat
     2521 tcttcaatga ttgccaagat gttagcttcg cctacgatga tgtacttttc atcgccatct
     2581 ttgacatcaa gacctgcgtg ggcttcaact aagacacggt ctccagtttt aacacttgga
     2641 gcaaccaagt caccgttcaa ggtacgaaca ccttgtccag tagccacaac ttgggctgtt
     2701 tttgtttttt cttgggctga acctgcgaga acaaagcccc caacggtttg ttctttttct
     2761 tctattttta agaccacacg gtcccctaat ggtttcaaca tctgatttcc tccataatga
     2821 gatagatatt attagcactc tttatacctg agtgctaatt catagttcta ttgtatcact
     2881 tagtcagaaa taatcaagaa aaaagtctga ctttctcaag ataaaaagcc tgagaccaac
     2941 tcagactttt taatgtttat actcaatgaa aatcaaagag caaactagga agctagccgc
     3001 aagctgctca aagcattgct ttgatgttgt ggataaagct aacgtggttt gaagagattt
     3061 tcgaagagta ttaaaatggc aattcttcct cttccaagac taaatctgcc aaatcttggc
     3121 ctgcattatt ttcacgcatg gcacgttgag cacgactttc caagagttgg aatcctgtta
     3181 caagtacttc ggtcacgtag ttcatttggc catttttctc aaagcgacgg gtacgcaatt
     3241 ctccatcaac ggaaatgaga ctacctttgg ttgcgtagct tgccaaagtt tctgctagtc
     3301 tgccccatag gaccatattg acaaaatcag cttcacgttc accgttttgg tctttgtaac
     3361 gacggttcac agcgatagtt gctcgcgcta ccgacttgtc attgttggtt ttgtgcaatt
     3421 ctggtgtaga cgttaaacgc ccaatcatga taactttatt atacatattt tcttcctcct
     3481 acttatctat tcgtaggaaa tcaaaaaaag ttacagaaat ttgtaacttt tcgaggaaat
     3541 ttcttatttt ttatgaatca tgaaacctgt cgcctgttga ttggccataa tggtcatatc
     3601 tgtaatctga acacggcgag gctgactggt cacatagact actgtgtctg caatatcctg
     3661 agcttgcaag gcttctattc cttggtaaac ggacgcagct cgctctttat caccatgaaa
     3721 acgaacagtt gagaaatctg tttcgacaat cccaggctga atggttgtca ccttgatatc
     3781 cgttgcgatg gtatcaattc gcagtccatc cgaaaaggtc ttaaccgcag ccttggtagc
     3841 tgagtaaaca gcggcaccag cataggcgta gattcctgcg gttgacccca tattgataat
     3901 atgaccttga ttgactttta tcattgctgg caagaaacag cgagtaaccg ccatcagacc
     3961 tttaacattg gtatccaaca tggtcaacat atccaactcc tcatagtctt gatagggagc
     4021 caagtcaaga gccagtccgg cgttgttgac caagatatca atcctcccta tcgtttctag
     4081 aatatcagag caaacagtct tcaccatgac catatccgtc acatctagtg gaaaagtcca
     4141 gacggtttga tttggaaagc ttactgcaaa ctccgacttg aggatttcta atctgtctat
     4201 ccgtcgtcct gttagaacga catcctcacc ctgctccaga taagcacgcg caatcgcttc
     4261 accgattcct gaggttgctc ccgtaatcac tacatttttt gccatcttat ttccttctat
     4321 cagatgctga caagttccta ggcagtccag tgtttcgctg ggtcgaacgg tgttccgaca
     4381 acttggtctt ctgataattc aagcacccca cgtttttgcg gagcatttgg caaatgcaat
     4441 tcacgaggac tacacatcat cccaaaactc ttttcaccac gaagttcgcc tgggaaaatg
     4501 aaattccctt ttggcatcat ggcaccagga agagccacaa tggttttcaa cccaacacgc
     4561 gcattaggtg ctcctgcaac gatttgcact atcttgtcac ttgcgactgc aacttggcag
     4621 atgttgaggt ggtcactgtc tggatgagct accatctcga caatttcacc aacaacaaac
     4681 ttaggttcct tatcattaac aatttcttct gtaaaacctt ccgcctgtaa ttcttggttc
     4741 aaacgagcga cttgctcatc tgataaaaag acttgaccgc gctctgcaat ttcaaacaag
     4801 ctcgaaacct caaaaatgtt ccaagccact gtttccccat tttctttgag gaaaacacgg
     4861 gctaccttgc ctttgcgttc cacattcagt ttggcatctc cgctattttt cacgatgacc
     4921 ataaggacat cactgacatg ttctttgtta tacgtaaaaa tcattgtttc ctttttctcc
     4981 tatttcaatc ctgctaaaaa atcgttaatt tgctccttac ttttacggtc gcgattgaca
     5041 aaacgaccga tttccttgtc cttttctaga acaacaaggc taggaattcc gtaaacatcc
     5101 cagagtttgg ccaaatccat atactgatct cggtccattc gaataaaggt gaactctgga
     5161 ttggtctctt caatctctgg taaggcagga taaatataac gacaatcgcc acaccagtct
     5221 gccacaaaaa ggaagacctt cttgcccgct ttttccacta aagatgctaa ttcttctaaa
     5281 cttgctggct gtatcataag acttcctcct catagactag gtcttcattt tcatagacaa
     5341 aggtataatg acggccatcc tcaaaaatga cgccaccaac caagctctcc agactgcttt
     5401 cgtaaacttg aacataaagg gtcgcaattt cccccatgtc ggaaaaatgg tctcgcacaa
     5461 tctctgtcaa ctcttcctga gtcttcatga gcttacggtc atctgcaact tttttcgtag
     5521 caagagcaag gcttccgata cctagcagag ccaagcctgc catccacatt tttttagctt
     5581 tcataccatt cattttaaca caaaaaaggc ttcaggacaa atgaggaagc agcagaaaag
     5641 caagtaaaaa gcctcttcct ttaaggaaaa ggacttctta tactaagagc aaactagaaa
     5701 gctagccgca ggctgctcaa aacacggttt tgaggttgta gataaggcga cgttgacgtg
     5761 gtttgaagag attttcaaag agtattattc ttattgccag gcacctaagt tgccaacgta
     5821 gtaaccatca ggtgtgtagg tattgcgaag catcttacct gatgaagcca gataatacta
     5881 cttgccattg tctttgaccc aatcattcgc aatcatggaa ccagaagaac ttacataata
     5941 ccattctccc ttgtcataaa cccaagtact gactttcatg gttcctgagc aattaaagga
     6001 aaaaaaactg tccaatatca ttcgtttttt aaaagcattc gacactacat catctacgct
     6061 gtagtacata gatacatcaa acgtaaaagc caaaatatcg aaaaatatac aacttttaaa
     6121 ggcagcattt cattttttaa agtctaacaa ccatagaact gaaatcagag caaggcatca
     6181 atttcctact cttagtacgc ctattttgat ggttgaagtg aagatttttt tatttagcgt
     6241 agacaatttc caacttccca aaagccacat ctccacggat aatcaaggtt ttgctggttg
     6301 gggctacagg agcgtcagcc ttagctgcac caaaggaggt ttctactttc aaatctacac
     6361 gccagtgttg tggaacatag acggttgcat tcccgaaggc cacttcaata tttaaagttg
     6421 caaaatcacc tagcatctct gcattatcat agtagatttt agcatcccca aaagcgactt
     6481 ccacttggtc atctacgaga tcttgatctt gcttatagaa ggtcccgcta ccaaaagcga
     6541 cttccttatc cgtgacgact gtttttttcc cattgtacca ccattttttt tcattccaga
     6601 acttacttga atgcgtcaga taaccaacac caagtaccac caagatgcta gcccaaataa
     6661 gagaatgatt ggtaactggt aacaagtcat aagcgtaatt tgcaatgatg agcgccagta
     6721 aacctgtaaa aactgccgaa gtgagatgac gtctaaggat ggactcaatg gacttataag
     6781 caaaaaaaac aatacctagt aaaggccata ttttaccatc caaagaagga attccaaaat
     6841 tcccttgcag caagatccaa gctgctaaaa ccaataaaac aataccaaat gctttctttt
     6901 tcatgttttt tacctcatgt ttcttagatt ttcttttagg agggattggt aatgccgtga
     6961 gacatgaacc tccttgtgcg tctgataaaa ggaaatggtg cccgttcctg aaaaggactt
     7021 gtccactgag taaatctgac ggatgtttgc gatcgttgac ttggaaactc gattaaaata
     7081 gcgaggcaag atagactcca actcatagag cttgaggcga acctcataag cttccttctg
     7141 gttatgagcg tagatcttgc tcccttctgt ttcaaagaag agaatttctg acaggtctag
     7201 ataatattca cctgtcccct tgtaaaaagt caacctcgga gcctttgact cttgcaagag
     7261 ccgttgcaag tctgcaattt catctgtcaa agtgggggtc ttgatgacaa tttcagtctc
     7321 ctctaaattg ccgtcaatct caattcgtaa cttcatcaca tctcctttct gactctacta
     7381 tatcaaagct aagttaggaa tacaagggca aaaaagcaag tggtcaattt gaagcagtaa
     7441 gtggttgtag gactagctta acttacatgt cagaatagga agaaaaggaa tccccacacc
     7501 aaaccacaag catagccaac caagacatcc ttgggataat gaacaccccc taaaaccctc
     7561 accaacccca gcaaagctga aaagatcaag catactaagc caataggtag actagcatgc
     7621 agacaggcca tggagatgat agttgccgaa aagacatggc gactgggcat cgaccgtccc
     7681 gaactatcct tatcaagaag gggacagata tcccaagttt cataaggtct cgggtgattg
     7741 attttcttac gaaacaggga caaggccaca aaacctgatg caggaataag cacataaacc
     7801 ccaacttgct gaccaagtcc tagttgcagg taagtggtga ctagcaagat cagatagacc
     7861 aggggcatgg cgactgtcat caaacgattg aaactgcgta ataggaataa aagaattgga
     7921 cgactagtta gtctggagct gatatttcga taccattctt gataattttt catttatgct
     7981 tctcatactt ttctagaact tgttgagatg ttttcccctt ggcaagccaa tccactagct
     8041 tgtccaagcg acgtatttcc tgcatcagag gatcatcaat ttcctcaata cggacaccgc
     8101 aaatcttccc tgtaattgca attcgttcag ggacataata tggtgcttgt ctaaaaaaat
     8161 ctccgtaagt aacatcccta tccagacaag caagaacatc actcacttca taccctgtca
     8221 accaactggt cacttggtga actgactctg cctttccacc ctttcgctca acttttgcaa
     8281 tcaaggctaa gtagacagca gcaaatttca tattgtataa tttctgactc atacttctct
     8341 ctttctatgt gtactagtat aaataagaaa aagaaggccg tcaagccttc ttttgattta
     8401 ttcttctgct tcatcttctg taaattgact attgtacaag tcagcgtaga agctaccttg
     8461 cgccatcagt tcctcatagt tgccttgctc gatgatattt ccatctttca tgaccaagat
     8521 caagtctgca tttcggatgg ttgacaagcg gtgggcaatg acaaaggatg tgcgtccttc
     8581 catcaaacgg tccatggctt tttggatcaa ttcctctgtc cgtgtgtcaa cagaagaagt
     8641 cgcctcatcc aaaatcaaaa gcggtgcatc cttaagaagg gcacgagcaa tagtcaatag
     8701 ttgtttttgt cctacagaca aggtcacggt gtcatccaag atggtatcat agccatctgg
     8761 caaggtcata ataaagtggt gaattcccac agccttacta gcttccatca ttcgttcatc
     8821 actaatccct atttgattat agatgagatt gtctcgaata gttccttcaa agagccaggt
     8881 atcctgcaag accattgaaa aggcatcatg cacttctgaa cgcgtcatag ccttggtatc
     8941 cacaccatca atgtgaatac ttcccttatc aatctcatag aatttcatca aaagattgac
     9001 aatggttgtc ttaccagtcc cagtcggccc aacaatggca accttttgac cagcatgagc
     9061 tgtcgcagag aagtcatagt cttgaacatt gacaccgtcc accagaattt ctcctgctga
     9121 cacgtcgtag aaacgtggaa tcagattgac cagagttgat ttaccagaac ctgttgaccc
     9181 aataaaggcc actgtttgac cagtttctgc tttaaagcta acatgttcaa taactgcctc
     9241 cgaatttgcc gcatagcgga aggtcacatc cttaaactcg acctgacctt tgaagttttc
     9301 atcagtcagc tgcacttgaa cagggttttg gatagaagaa tgcaaatata aaacttgatt
     9361 aatccgctta gcagagacca tagttcgggg aagaacgatg aagagtgctc ccatgagaag
     9421 gaagcccatg acaacctaca tggcataaga catgaaaaca atcatgtcac taaagagagg
     9481 cagacgcgct atcggagcag cgtcgttaat cacataggcc ccaatccagt aaatcgccac
     9541 actcaaacca cttgaaatcc ccatcatgat aggattcaaa atagccataa gacggttgac
     9601 aaacaaattc aaacgggtca attcatcatt tactgctgca aatttttcat tttgataatc
     9661 ctctgcattg taggcacgaa cgacacgaat acctgttaaa ctctcacgag tgatactgtt
     9721 cagtttatct gtcagcccct gaatcaagga ctgttttgga aaggctagcg tcatcaaaac
     9781 ggtcgtcatc aggacgttga caatcactgc cacaagtacg gcccagagcc agtattctga
     9841 atgacctaaa atcttcccaa tagcccagat agccataatt ggaccacgcg ttaccacttg
     9901 caagcccata gtaatcaaca tttgaacttg agtaatgtca ttggtagtac gcgtcaagag
     9961 gctaggaatt gaaaatttct taatctctgt ctgcgagtaa tccaaaactc ggttaaaaat
    10021 atcacttctc agcctactaa tataaaaagc cgccactcgg gatgcaaaaa atccaactgc
    10081 aactacggac aagaaggcaa gaaagggcat tcccatcatc atgcttgccg gctgccacaa
    10141 ctcatctaaa ttagttcctt gactacctag caaatccgta attttcgaga tataggtcgg
    10201 cacttccaac tctagataga ccgaaaagca agtaaagaga atggctagta aaatcatccc
    10261 ccattctttt ctactaattc ttttggctaa tttctttatt ctctcctcct attcccttga
    10321 tattttgcct gtagttgacc gagaactttc tcaaaaatca gtaattcatc ttcatcaatg
    10381 tcttccatca actgcttgtc tatgcgttca aaaaaagcct taacctgttg catctgagaa
    10441 cgtgctttgt ccgtcagacg aacaaactta gcccgcttat caacaggact cgcctccaat
    10501 tccaccaaac cattttacac tatacgctta accagattac tagcaacaga cttggtaatt
    10561 ttgagttcct gctcgatatc tttaatcaag accaagtctt ggtttttctc gcgattatcc
    10621 caaaaacgca caacctgacc ttgcggccca cccataaatt caatgccgca acgtttggct
    10681 tccttttgca ccatcaggta agtttgatga ccaaaacgct taaagactaa catcggttta
    10741 tccataatct cccccttcta aataaaaata gttctctgga gaataattaa atttctatga
    10801 gaactatttt cttgattaaa aaaatcccaa gtgattttct cacttaggat catgttctat
    10861 aggttaaatt aaaacccatc tacgttcgta taaatctttt ggacgtcttc gtcgtcttca
    10921 agaacgctgt aaagtttttc aaaagtttca aggtcttcgc ctgacaattc cacttctgac
    10981 tgaggaatca tttccaattc agtcacttgg aattcttcaa taccagactc acggagggca
    11041 acgatagcct tgtgaaggtc agttggcgct gtgtaaactg tgattgtacc ttcttgtgct
    11101 tctacgtcat ccacatccac atccgcttcg agcaattgct caaagactgc gtctgcatct
    11161 tcacctccaa atacaataac acctttgttg tcaaagaggt aagaaacaga acctgaagcg
    11221 cccatgtttc cgccgttttt accaaaggct gcacggacat tggctgctgt acggttaacg
    11281 ttagaagtca aagtatccac aattagcata gagccatttg gcccaaaacc ttcgtaacgt
    11341 ccttctgtaa aggtttcgtc tgtgtttcct ttggctttat caatcgcttt atcgataacg
    11401 tgttttggca cttgggcttg tttagcacgg tcgataacga atttcaaagc tgagtttgat
    11461 tctggatctg gatcaccttt tttagctgct acatagattt ctacaccaaa ttttgcatat
    11521 actttagagt tggctccatc tttagccgtt ttcttggcta cgatattggc ccatttacgt
    11581 cccattagga atctcctttt ttcacatttt aatctttctt attataacac aagttttttt
    11641 gattttcact agaggaaatg gattttatta gcaaatcaag ctaggatagt actttacctg
    11701 ctaagatggt cttgcctttc tatctttatc aacagacact catccacatt caaaaaacaa
    11761 actagaccat tatctgcaaa tagaaagttt cagccaagtt tgacaaagtc agctcaaatt
    11821 actgtttgaa gtttgtagat ataagcgaca aaaacaatca tactgcacct tttgttgaca
    11881 gtctactcca gacatatcat agttccagta aatactttgc aatccaacag ttcttatagg
    11941 cgctattgta ttctaagaaa ttaatagacg agtttctaag caaacctcta atactcaata
    12001 aaaatcaaag agcaaactag aaagctagcc gcaggttgct caaaacactg ttttgaggtt
    12061 gcggataggg ctgacctggt ttgaagagat tttcgaagag tataatttac gtgttcccaa
    12121 gatggagaag ttagactagt acactggcac ttctaaacca ttgctagcaa ttgatttgtt
    12181 catatttaat ttcatttctt ccataaatgg gtattagata taaacagcaa aatatttccg
    12241 atacgtgtcg ttcttgaatt tccaatcatc taaaacaagt aaaggataat caatcccctg
    12301 tatatcaagg aattggctac cctttttact tttttacaca ttctgtttga tagattcatt
    12361 ttaacatcac gagcatactc caatggaaat cgctaggcaa gagataaact ttcagatatc
    12421 cgcagagaga tcatcgcctc tttttgtcgc aagcattctc ctctcctagt cattttctac
    12481 cttatcttct acctgaggat agagagttgt tccccaaata gaaatcgtcc gcttacgcac
    12541 tagtggcaaa tcggtttttt cataaaccgt acgccaccat tcccaggcaa gcccggtaca
    12601 ctctctaatt ttgacagaga gattacgaac attccctttt aaaggaatac tagtggtaaa
    12661 gtgagccgtt aaatcctgcc catttctgtc ccaagcctta ggagtcaaga cttccttacc
    12721 ttgatgatca taggataatt cattccaagt aatataatat tgggcaacat aggcaccact
    12781 atgatccagc agtaaatctc cgtttctgta agctgtaacc ttagtctcaa catagtctgt
    12841 actattttga aaggtcgcaa ctacattgtc acgtaaaaaa gaagttgtat aggaaatcgg
    12901 caagcctgga tgatctgctg taaagcgact gccttcttga atcaagtcct ctaccatatc
    12961 caccttgcct gttacaactc gggcacccga acttgggtcg ccccctaaaa taaccgcctt
    13021 cacttctgta ttgtccaaaa tctgcttcca ctctgtctga ggagctacct tgactccttt
    13081 tatcaaagct tcaaaagcag cctctacttc atcactctta ctcgtggttt ccaacttgag
    13141 atagacttgg cgcccataag caacactcga aatatagacc aaaggacgct ctgcagaaat
    13201 tcctctctgt tttaaatcct ctaccgttac agtatcttga aacacatctc ctggattttt
    13261 aacagcgtct acgctgactg tataataaat ctgcttaaaa ttaacaatct gaatctgctt
    13321 ttcgcctgaa tggacagagt taaaatcaat atcaagagaa ttccctgtct tttcaaagtc
    13381 agaaccaaac ttgaccttga gttgttccat gctgtgagcc gtgatttttt catactgcat
    13441 tctagctggg acattattga cctgaccata atcctgatgc cacttagcca acaaatcgtt
    13501 taccgctccg cgaacacttg aattgctggg gtcttccact tggagaaagc tatcgctact
    13561 tgccaaacca ggcaaatcaa tactataagt catcggagca cgatcgaccg caagaagagt
    13621 gggattattc tctaacaagg tctcatccac tacgagaagt gctccaggat agaggcgact
    13681 gtcgttggta gctgttacag aaatatcact tgtatttgtc gacaagctcc gcttctttct
    13741 ttcgataaca acaaactcat cgggtagctg attaccctct ttgatgaaac gattttcaat
    13801 actttctccc tgatgggtca agagtttctt tttatcgtaa ttcatagcta gtataaagtc
    13861 atttactgct ttatttgcca tcttctacct cctaataagt tcctggattg agttgcataa
    13921 actcagactt gttcagcgaa atcagccgtg gttggactaa gtaatccaaa atttcctcgt
    13981 acaattcttc tgagacattg cgtcgccgtc tggctagata agaagtcgga atgaccgtat
    14041 tatccaacat aaatacctta tctaagtcaa tcaaggttgg tcttgtaaaa ggattacgag
    14101 ctagatctgg ctcttctatc ataaagttct tgaccaaacg tctggtcaag agagctggtt
    14161 tgaaggtctg atttttaacc aactctttgt ttttagtcat gctgttgtca atacagatat
    14221 acatatgatt cttcacagcc aaatcgctac taatagtcgg aaaaggcaaa taaagagcta
    14281 caacatctcc tctcttaatc aagcaagagc accccctttt ctcctaatgt aacatagaca
    14341 ggattgacca agtcttctga ttgactcaga atttccaaag tttgagtttg gcgcgctgtc
    14401 aatttagtag catcttgtct cttcaataca aaatgcttgt cgccaataac cttgacaata
    14461 taatccttct ccaaagctga ctggtaaatc cacatcagat gttgtctgtc ctgagaactc
    14521 aagagagaag gattttcaag cctcccgata gtctgataaa aatcaaaaac aggagctaac
    14581 tcctgccaat ctgattggct agttgtcaag gctagaaaaa gggctttgcg agctgatact
    14641 tcttggttag ccttgagagt tactttcccc tccaagtttt ttagaaatcg ggaaactcca
    14701 gaaagcaaat ttttctctaa ctgcgagaaa taaaaacctt tcgttcccag acataagtct
    14761 ttcatgtcgc tttctctagc aaataagagc tcaaacattt gatagtaaaa gaaaaatatc
    14821 tggcactggg tcgcgctcat cttttcctta tcggcttctt tttttaacca gagcaagggc
    14881 gacaggtagc tggattgaga catttcctct acctcctact cttttttaac tggagcatct
    14941 gcactagctg ccacttcttt tgactggata ctttcccact ggttaatctc ctctgagata
    15001 agaccttcgc atgtcttgac aaatagggca aaagccttgg tctttcctgc atatttctcc
    15061 gtttggcatt gatagaggaa tttttctttc tccaggagtt gcgcagtttt ttggtaagaa
    15121 atccaatttt cctttgcatt atacaaattg ataatcccct cacacagcaa gccgagactg
    15181 gataaggcaa ccgaaatcaa acggtagcga tcacctggca taggaatagc acaaaagaca
    15241 gctatgagga aacctgccac gatttctgtt atttttaata ccttatagcg cctacgatgt
    15301 tgaacgcttt tctttaaaaa atgagctatc tgtacgtcta atcgctctgt caggtacatt
    15361 tcttctggcg tcatattcgt aactcctttc atttactttg ataatcatag tataacatat
    15421 ttgaatatca aagtatagta aaaactggac tataacttat tagacttcct gcgaaacaaa
    15481 atatggtata gtagttctat gaattatgaa gcaagtaaac aattaactga tacacgattt
    15541 aagctccttg ttggtgttca gcgtaccact tttaaagaga tgttagctgg attaaaaaca
    15601 gcttatcaaa aaa
//
LOCUS       DAVBGY010000046        15154 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVBGY010000046 DAVBGY010000000
VERSION     DAVBGY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15154
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.046"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(62..859)
                     /gene="proC"
                     /locus_tag="VQ620_001517"
     CDS             complement(62..859)
                     /gene="proC"
                     /locus_tag="VQ620_001517"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET2764114.1"
                     /translation="MKIGFIGLGNMGASLTKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(863..2125)
                     /locus_tag="VQ620_001518"
     CDS             complement(863..2125)
                     /locus_tag="VQ620_001518"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET2764115.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAVSFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            complement(2135..3265)
                     /locus_tag="VQ620_001519"
     CDS             complement(2135..3265)
                     /locus_tag="VQ620_001519"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET2764116.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMVEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRFQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(3345..5228)
                     /gene="cbpE"
                     /locus_tag="VQ620_001520"
                     /gene_synonym="pce"
     CDS             complement(3345..5228)
                     /gene="cbpE"
                     /locus_tag="VQ620_001520"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HET2764117.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAETGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKERWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAVGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(5231..6118)
                     /locus_tag="VQ620_001521"
     CDS             complement(5231..6118)
                     /locus_tag="VQ620_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2764118.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(6108..6569)
                     /locus_tag="VQ620_001522"
     CDS             complement(6108..6569)
                     /locus_tag="VQ620_001522"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET2764119.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(6566..7474)
                     /locus_tag="VQ620_001523"
     CDS             complement(6566..7474)
                     /locus_tag="VQ620_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET2764120.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            7467..7664
                     /locus_tag="VQ620_001524"
     CDS             7467..7664
                     /locus_tag="VQ620_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2764121.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            complement(7716..8387)
                     /locus_tag="VQ620_001525"
     CDS             complement(7716..8387)
                     /locus_tag="VQ620_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2764122.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRLFNYLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(8441..8662)
                     /locus_tag="VQ620_001526"
     CDS             complement(8441..8662)
                     /locus_tag="VQ620_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HET2764123.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDSGYIGGGNHLCKGSAARF"
     gene            8934..9743
                     /locus_tag="VQ620_001527"
     CDS             8934..9743
                     /locus_tag="VQ620_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2764124.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(9924..10799)
                     /gene="aguB"
                     /locus_tag="VQ620_001528"
     CDS             complement(9924..10799)
                     /gene="aguB"
                     /locus_tag="VQ620_001528"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HET2764125.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(10809..11894)
                     /gene="aguA"
                     /locus_tag="VQ620_001529"
     CDS             complement(10809..11894)
                     /gene="aguA"
                     /locus_tag="VQ620_001529"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HET2764126.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRSF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALEKPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLLGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(11891..13018)
                     /gene="nspC"
                     /locus_tag="VQ620_001530"
     CDS             complement(11891..13018)
                     /gene="nspC"
                     /locus_tag="VQ620_001530"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HET2764127.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(13018..14277)
                     /locus_tag="VQ620_001531"
     CDS             complement(13018..14277)
                     /locus_tag="VQ620_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HET2764128.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVITLIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVKKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(14274..15134)
                     /gene="speE"
                     /locus_tag="VQ620_001532"
     CDS             complement(14274..15134)
                     /gene="speE"
                     /locus_tag="VQ620_001532"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET2764129.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
BASE COUNT         4439 a         3505 c         2767 g         4443 t
ORIGIN      
        1 agtttgaatt tgattttcga agagtatcag ccaccataaa agaggcagat aaaactacct
       61 cttatttacc tagttcttgt gttcgtttat aggcttgatg aactgcctcc atgactgtgc
      121 ctcgaaaagc atgcgcttct aggcttgcta cgccagcaat agtcgaaccg cctggactac
      181 aaacttgatc tttcaaaact ccaggatgtt gctggctttc taggaccaat tgcccagctc
      241 ctaccacagt ttgggctgcc attttcaagg ctgtttctcg tggtaatccc gtctgaacac
      301 ctgcatctgc caaggcctca ataaagagat agacaaaggc tggtccacaa cctgcaagac
      361 ctgtcgctgc attgattaag ctttctccta attcaaccaa gagaccagcc ttggctaaaa
      421 gctgacaaaa gatctcactg tcctcagccc tgcaattagg agacaaggca taactaatca
      481 ctccttgccc gatagaagca ggggtattag gcatcatacg aataattcgg tgttggcttg
      541 ggataagact tgctagtttt tctaaggtca atccagctgc catcgaaatc aaaagaagac
      601 tttctctttt ttcaaggatg gtctggtatt gagaaagcag ttcagaaaac tgagcaggct
      661 taacacctag aaaaatcaca tctgcttctt cgaatatttc ttcattgtta gaagtctgac
      721 caccgaagtt agcgatgaaa gcatccacct tgacttgact acgattggca aggagaatct
      781 catctgacgg tctagcctgc aagacagact tggttaaact agcacccata ttccccaaac
      841 cgataaatcc aatcttcatc tcttactccc ttatctgtcc atcaccagtg accacatact
      901 tgtagctagt caattccttc aagcccatag gaccacgcgc gtgcaatttc tgagtagaaa
      961 tccccatttc acaaccaaga ccaaattgtc ctccatctgt gaaacgagtt gaggcattaa
     1021 catacaccgc tgcagagtcc acttgatctg taaagtatgc tgcagcttca gcattttccg
     1081 tcacaatggc atccgaatga tgggtactgt gggattcaat atgcgcaacc gcttcttcta
     1141 aactgctcac aaccttaaca gctagaatat agtctaaaaa ctcggtatca aagtcttgtg
     1201 cttgagcagc ttgacctgaa acaaactggc ttgctttgct atctaggcgg aattgaattg
     1261 gttccaaccc agcttctttt cgatctgcaa ccaacacttg ctccaagcga ggaaggaagc
     1321 ttactgcttt gttttcatga accagcagaa cctccatggc attacaaaca gaaggacgac
     1381 tggttttagc attgttgatg atagacagcg ccttgtcttc gtctgcatcc ttatccacat
     1441 agacatggac aatcccagtt cctgtctcga taacaggtac aatcgcattc tcaaccactg
     1501 cattgatcaa gccagctcct ccacgaggaa tgagaaggtc tagatagccc ttggccttca
     1561 tcatagcata actactttca cggctagtat cctccaccag ttgaatcaca tttggatgaa
     1621 tagtagtcgt ctccaagccc ttcttcaagg ctgtgacaat ggcatgggtt gtttgatagg
     1681 catccttacc actacgaaga acaaccgcat ttccactctt aagagtcaaa gcagccgcat
     1741 cagacgtcac atttggacgg ctttcataga taataccgat gacacccata gctacacgtt
     1801 tttttgtgat aaccaaacca ttttcaagct gacttgtttc taaaacttca ccgattggat
     1861 ctggtaaggc aaccacttca cgaattcctc ttgccatcgc ttctatacga tctgcatcca
     1921 aataaagacg atccaacatc acatctgaga ttttcccctt agccgctgcc atatcgaggg
     1981 cattagccgc taaaatttcc tcagtagcag ccactaagtg atcagccatg gctagcaagg
     2041 cttggttttt cacttcttca ctagctgtgt taatcgattt tttaacagcc tgtacctgtt
     2101 caaattgttc ttgtctactc accatagttt acctctaaaa ttctgtaaaa agtagttgga
     2161 tttcaggagt aatggaaatc cagtcgtcac ggtaaatcaa gacacccttg gctttttgaa
     2221 aacgcaacat atcctccaaa gcagatgctc caaattgcac gcgtcctttt ccaagtgatt
     2281 ttccactttc cttatcaaat actgtcacga tatcaccgta agaaaagact ccttctgctt
     2341 caacgatacc agataagaga agactctttc catgttgaga gagagcttcc gcagcccctt
     2401 tatcaaccca aatagaacct tgactctgag catagaaggc aagccattgt ttctgggtac
     2461 gaagcccctt ctcttgagca acaaagtaag aaccatcctc ggtctcctct gccgcctcaa
     2521 ccatggaatc tgatttcaag gatgagcaga tataaacagg aactcctgat tccgtcgcga
     2581 tagttgcagc cttgattttg gttaacatac ccccagttcc gtttgacgaa ccagctccac
     2641 cagccatatc aataatctca cgattgatgg tctcgattct ctccaagcgt ttggctcttg
     2701 gatctgaatt aggatttcca gtatagagac cgtccacatc tgtcaagaaa actaaaaggt
     2761 ctgcttggac catggccgct acttgagcac ttagagtgtc attgtcccca accttgagct
     2821 catcaataac gacactatca ttctcattga tgataggaat tgccccacgg ttgagcaaaa
     2881 ccgacaaagc ctgatgggca tttttataac gacgcttatc cacaaagtca tcttgggtca
     2941 gcaagatttg tgcagaaacg atttgacgca agagaagatt ggttgtatat tcttccaaca
     3001 aaagcccctg ccctaccgct gctgaagcct gtttatcagc aattttagtc ggacgctttt
     3061 taaatcctaa ggctccaaaa ccagctgcaa tggcacctga agacaccaaa atcaactcat
     3121 gaccagcctc gtgtagcata gccaactgct gggtaatatc ctttacctta ctacgtgata
     3181 aacttccatc ctcatttgtc agagaagaag tacccacctt aaagacaatc cgtttgtatt
     3241 tcatagtctc actccgatta ttcatattta tccattataa catgaataac aggaaataga
     3301 aaagagttga taggaaaaag aaggccctaa aaccttcttt tctactactg ttctgattcc
     3361 gatttgttta cactcgtttc agagctttgt gaagttgagg atgtcaaatc ttgactagtt
     3421 gaagaatttg taacactttc atgttgactc gtttctttat tttcaagatc ctttttcact
     3481 acttctttgg attcttttat ttctgaatga cttgtagttt taattgtagc agatttctcg
     3541 atcggaacat ccactaacca ggcaccactt gaatccacag tatagccttc tggtgtctta
     3601 ccattcacaa gtaattgacc attttctttc aagaagtacc atttatcctt gtccttgatc
     3661 caacccacag ctcgtgaacc atctgtcttg tagaaatacc aatcatttgc ctttttaagc
     3721 cagccttgct tcatagctcc tgattcttct agatagtaat gatgtccagc aacttctatc
     3781 tcacctgtct tcataatacc agtagaatcc atatagtacc aggcttcttt atcttttacc
     3841 cagcccgttt tcatttcccc tgatggagca aagtaatacc attgcccctt atattgaagc
     3901 caacctgtct tcatagagcc atcaacatcc aaataatacc accgctcttt atcttgaatc
     3961 caaccaattt ccatctggtt ttctttgtta aaataatacc agattccagc gattttcttc
     4021 caatttttag cagcaacacc agagtctgtc aaatagaacc aacgattgtt ccattttttc
     4081 cattgattct gtaataagat acccgtttgg ttaaagtaat accattcacc cttaatttca
     4141 ttccaaccga cagcatattc tcctgtagaa tcaggcgctt gataccacca gtttccatat
     4201 gaactcttat gccaaccagc ttgaaaactt ggaataggct tgtaggaagt tgaaatgttt
     4261 actaaaccgt cttgtcgaat atcaaaaact gttgcatcat agtctttgct ggctgcgttg
     4321 attctctcaa ttcctcgttc tttgagccaa ttaacatact cactatcaac accatttttc
     4381 caaggtagac tatccgaagt ttgaacaatc aaactcggac tcaaattttt aatgaaatcc
     4441 ttggtatttg atttgttggt atcatggtga tgattaaact tcatcaaatc aacttttcca
     4501 atgagaggac catacttgtc ttctgctcca tgaacattat ctaagtcgcc cccaaggtaa
     4561 attttcttgc cattgacttt taccacacta atcaaggaat tggaattatc atcccaaatt
     4621 ttctttaatt cacctgatga atcggtttca ttttcataat tatagagctg aatatccatg
     4681 tccccaaact gaaaatgagc atccccttgt gtgatatttt gaataactga aacacctgtt
     4741 tctgcagcag tctgtaaaac cttatcatag ccatacagat tatcccatag acgttcagaa
     4801 ttagtaatac gactatcact atatttctta agatagactc ggtcaactgg ataggtagac
     4861 agtaattcat caacatttcc aatatgatca ctgtgggtat gggtcaccaa aataaaatca
     4921 agtttttgga cacccaattc cttcaaacga cgaaagacac ggtctgttag aacatgctta
     4981 taagacgttt caattccttc tctccatgga tagcgagaat cacttccatc tgggaaatca
     5041 taatcttctc ctgtatccac catggcaaaa tgtccattgc tttcaagaat aatcgcatca
     5101 ctgccacctt cttgaacatt gataaagtga attttatttc ctgaactttc ttgagcctga
     5161 acattcccat accatgacaa acctaaaaaa gcgcctacaa gtgctaaact agttaatttc
     5221 tttttcattc ttactttctc aatctctcca aggtttcctt aaaaatctct gggatatctg
     5281 ctttaaattc caaggtctta cctgttctcg gatgagtaaa acctaaagtc ttggcatgaa
     5341 gaaattgtcc atgtcctttc aaagtcttgc gaggaccata gacctcatca ccagcgactg
     5401 gatggccgat ataagccatg tggacacgga tttgatgagt gcgccctgtc tccagttgca
     5461 actctactaa gctataatcg ccaaagcgtt ccaagacgtg aaaacgcgtc actgcaggct
     5521 tccctttagc agttacagcc tgtttcttac ggtctttttc actccggcca atcggcgctt
     5581 caattacacc acgatcatta ggcagatttc catgaacaat cgcccaatat ttgcggagag
     5641 actttttatc cttgagttct tgggcaagtg ctagatgcgc atcatcgttt ttagcaatca
     5701 tgagaagacc tgacgtatcc ttatcaatac ggtgaacaat acctggacgc agaaccccat
     5761 tgatacccga caagtcctta atatgataca tgagggcatt tactagggtt ccactggtat
     5821 gaccagcact cgggtgcaca accattccct gaggtttgtt aacgacagcc acatcctcat
     5881 cttggtagac tatttctagc ggaagatcct cagccacata ctctaatacc tctggttctg
     5941 gcacatggta agtgacgaca tcaccctctt ggactgtgta tttagctttc ttgacttgac
     6001 cattgaccaa gacctggcct gatttaattt gttcattcgc gagactacgt gataattctg
     6061 acaaatctga caaagcctta tccaaacgca gaccaccagt ttcaatttta atttccattt
     6121 atttcctctt ttagcattgc aatcaataaa ataatcactc caaccgtcag atagctatct
     6181 gccacattga aaattgcaaa gttgataaag tcaaggtgga acatatccac aacaaagccc
     6241 tgactgaccc tgtcaataaa gtttccaaga ccacccgcga ttattagagt caaacccaag
     6301 accatccaga atgagtcctc catgtgttta tgtaaatacc aaatggcacc tatcacgaca
     6361 accagagtaa tgacagcgaa taacaactgc tgatcttgta agatagaaaa ggctgcacct
     6421 cgattttgca ggtaggtcaa gctaacgaaa ttggggatcc aggagcgcac ttcacccagt
     6481 ggaatctgct ggacgatata ggatttgacc aactgatcca gcccaatcaa aagcagtaca
     6541 atgactgcca ctattgctct ttttttcatg atttcctctt ttgatcaaaa tattcttgca
     6601 tgacttctac gaagagagtc ccagcttgac taagctccac ttcttcacgt ttaacataga
     6661 ccatgcggtt atctaggtta tccttgagac gaataactgt aatgccatta acactgtcac
     6721 tatctaaaaa tccagaacct gtcgcatagg cgtccgtccg ctccaaaata ccattcaagg
     6781 tggcacggtc tgtcacatta aacatctgtg agctagcgct ggtatcaaca aagttctctg
     6841 aataataaag gtactcgtct ttctcttgag tgaaacgaac cgttggtaaa tccgctaaat
     6901 cctccatgac taattcctct ttctgggcta aaggatgacc ctcacagaga taaatatggg
     6961 tatggaaagg aatcaattcg atgacctcca gacctaactt ttcaacccgt tgcataatcc
     7021 cctttttatt ttgattgttg aggtagataa tcccaatctc actatgccct tgcgccactt
     7081 catctaatat ttgaacagta gttgattcaa aaatacggaa gttcttatag tcaggatagc
     7141 gctctgaaaa ggccgtaata gttggtggta agaagtcata gtgctggcta gcaacggaaa
     7201 attcatcttt ttcttcttca ggattggcat actgattttg aaaaatatca aatcctttaa
     7261 ccaattcttg tgctttttca taaaattcca tcccacgacg ggtcaagaaa gtccctgagc
     7321 tggtccgacg gaaaatctta aagcccaact ctttttccaa atcacgaaca gaaatagaca
     7381 gactcggctg actaacatac atcttttcag cagcttcacg aaaagtacca ctattggcaa
     7441 tagccacaac atagcgtaat tgttgaatgt tcatcttcta cccccaactt ctttatcttt
     7501 tcattatacc atattttaga agttttccaa aaaggaaaaa agaacatcct attcttctta
     7561 actatcttca ctatctgcct ttctcacgcc aatcttattt tcaaaatcaa ttcaaaaaaa
     7621 taagtggcta cacaccactt cagtatcgga aagaaaaacg ttgactattt gtgaaaaaag
     7681 aaaatgccgg aaaattccga cattatttta gtaagctaac ttcctgaaaa taggagcaac
     7741 cacacatccc agaaggcagt gatattaatg agaatatgaa ctagtattgg atagtagaga
     7801 tttttagtca tgcgatacaa tagagctaaa ataagacctc cactagcata aatgaaaaaa
     7861 gcaagaggag ttaaagcaaa attgatatga atataaccga aaataatagc agaaagcaaa
     7921 acatctccgt accaaggtga gtttttgaaa aaggttgtca taagcacacc tcgataaatc
     7981 aattcctcag caataggggc gatgaagcaa acgatgagca agaaataagg gaactcctgt
     8041 ctccccatca tttctaccgt ttcattcaaa gaaatctgat ttgaagacag ggatatgaaa
     8101 tacgaaaaga ggaagtcaga catatatgaa atgatgtagc ccagtaaaag gtagatgaag
     8161 tacctcagct gccacttgaa atgaaaaatt ttctttcctg caaaaaccag tagatagatg
     8221 accgctaaga aaagaactcc gctctccatc aaaagcagaa tctgaaaaaa ttcacgactt
     8281 gctggtagat agggctttgc gagataattg aaaagcctcc aaaaccaagt tgatttgtaa
     8341 aaaattaagg acaatgctag taaaatttga atagctcgtt ttttcatatt aaatcctctg
     8401 ttttctcccc ttgtttcttc atattcctaa taaattttac ttaaaatctc gcagcacttc
     8461 ctttgcaaag atgattacct cctccaatat atcctgaatc ataggtgcct ctgggacaaa
     8521 atccatgaac cggcagagtt aatactggtt ctatttccca atcgtaccta ccattaggat
     8581 ttttaccata tgaatctgct ccaccttgta tcagctctaa ttcttcattt gttagattta
     8641 tattttttgt cattaaaatc atgatatatt tcctccatta ttcttcagat tagttgagta
     8701 atctgatact catacctact tacaaaaaaa ctattatatt aagttggttt tttaatttgt
     8761 ctagttgcaa cattgacaaa catagtatag catatctttg ccaaatatcc tcttcaaatc
     8821 atgaattgtc atcaaaacat cttaaactat aaaatcaatt agtctcaagc tttctatcaa
     8881 tttcttctcc aaaatatgct ataatactac caaaagataa agaaggaaga cctatgatta
     8941 aactactagc cttggatatg gatggaaccc tcctcaatga agccaaggaa atcccacaag
     9001 ctcacattac tgctattcac aaagccattg aaaaaggtgt caaactggtt ctctgtacgg
     9061 gtcgccccct tttcggtgtc ctcccctact acaaaaaact ggggcttgac ctccagaacg
     9121 aatatgttat tgttaacaac ggttgttcaa ctcaccaaac cagtgattgg ggtctagttg
     9181 actggaaaga actgagtcca gctgacatcg aatacctcta tgaccttgct gaaaagagtg
     9241 atgttcagtt gacacttttt gacgagtcac attattttgt tctcggtggc aagcccaatc
     9301 aagttattga aaatgatgct aaactagtat tttcagacct gactgaaatt tctcttgagg
     9361 aagcgactag tggaaagttc cggatgttcc aaggtatgtt tttaggaaca aaagaacaaa
     9421 cagacgattt tgagcagcgt tttgctgaag agctttgcca acgattcagt ggagttcgtt
     9481 cgcagcctgt catttatgaa gcaatgccac ttggaacgac aaaggctact gctctttcac
     9541 gactagctga gattttgaag attgattcct cagagattat ggccatgggc gatgctaata
     9601 acgatatcga aatgctccag tttgcagggc ttgggattgc aatgggaaat gccagcgatt
     9661 atgtcaaatc tcttgcggat gccgttacct caagcaacga agaagacggc gttgcgcgtg
     9721 ctattgagaa atatattcta taaaaaagaa aagcaaatag acagaagtta ctgctatttg
     9781 cttttttgct aatattttaa aatacggact aatcaataga caagaatagc gaatcataaa
     9841 aatcgattta ctagatgcca tagtatctta tagttgctaa taagaagtta gtctagcaga
     9901 atgaatctct catttctccc acactaatct gtaatttgtc tatacatttc tggtcttcta
     9961 tctcgaaaca agccccagtt taggcgttca ctcgctccct tgtctaggtc ataagtagct
    10021 aacagaacag cttcttcttg tctttcagct cgttctagaa tagctcctgt ttcatccgtc
    10081 ataaaggagg aaccgtagaa gtcaagactg gaactctgtc cgccattttc ctcactagga
    10141 gtaacctcct ctaaaccata acgattggct gcgatgactg gaacaatatt cgctgctgcg
    10201 tgcccttgca tagtacgttg ccagtgacca caactatctg tatccaaaat cggctctgaa
    10261 ccgatggctg ttggataaaa gagcaattct gccccattca gagcaagaca gcgggctgtt
    10321 tcggggaacc attgatccca acagataccg ataccaatct tagcatagcg agtattccag
    10381 accttgaaac cagtgttacc aggcgtaaaa tagaattttt cttgataata atggtcatct
    10441 ggtatgtggg tctttcgata aacgcccagc acttccccat ctgcatcaat aacggcaata
    10501 gagttataca agacattacc atctttttca tagaaactga ttggtaaaac aacttgtagt
    10561 tccttagcaa tcaccttaaa atgctgaatg gcagtatttt ccgctacaga ttgggcatac
    10621 tggtagtagt catactgacg ttcctgacag aaatagggat gttcaaacaa ctcgggcaag
    10681 agaataattt gggctccttg ctcagcagcc tgacgtacta aacgctctgc ggtttggata
    10741 tttgttgcca catccttagc gcattgcatc tgaatggttg caactcttac atttctcatc
    10801 tttttctcct attctggaat ttgttgggtg atacagtgga tattgccacc acctaagaga
    10861 atatctctgg ctggtattcc gacaacttta cggtctggga aacacttgct gaggatatct
    10921 aaggccactt ggtcgtttac atcctcaaac tgtggaacca agacagcctt gttggcgata
    10981 taaaagttta cgtaggaagc tgctagtcgt tcacctgcgt atcgcttttc ttctccttct
    11041 tcatagatgt agcctagcaa atcttcttct gtcacaactt gtcgaactgc agggataggc
    11101 aatttatgaa tggtgaagtg acaacctttt gcatctgttt cctgttctaa gagttcgaga
    11161 tctgcttttg acatggcata ctggggatca ttttcgtcat ctgtccaagc taaaacaacc
    11221 tcagcaggac caacaaaggc agcaacatta tcgacgtgtt cattggtttc atcctgataa
    11281 ataccataag gaagccaaat aactttttca gcaccaagac tttctaataa tgtgttttca
    11341 atctcctctt tagtcaggtt aggattgcga ccaggactaa gcaagcaact ttcagttacg
    11401 agaatagttc cttgaccatc gctatggatt gcgcctcctt ccagtacaaa aggtttagca
    11461 tcatagacag gcttttccaa ggcctcagca aaacgactgg ctacttggtc atcctcttca
    11521 taatcttgat aaagaccatc ataggtgcct ccccaagcat tgaaggccca atccacggct
    11581 aatttcttac ctttatcatt gacgagaatg gttgggccag tatcacgcgc ccaggcatca
    11641 ttggtgggaa tgtctaaata aacaaccttg tctccaagat aggattgggc ttcagataga
    11701 taggcctgct ccaccaaaag atagactctt tccccttctg ctatggtctc gatgatctga
    11761 gtaaatgatc ttttagcagc ctttccttga aaaggccatg atcctggtcg agtcggccat
    11821 atcatgaggg taccatgatg gggttcgtac tctgctggca tgtgatagcc taattttttt
    11881 ggactgtcca tcatgataat ctccctttaa agtcttgata gccaaaagct ttgagtaagc
    11941 tacagtctcc ctgttcgtcc atgagataga gacttggcaa tccaatacca ttaaaggtat
    12001 tatttttgac aaaagaataa atggccatgt cttgaaaata aagtctgtct ccgatttgga
    12061 ctggattttc aaaactataa tcaccaatca catcgccagt cagacaggta ttagaagaaa
    12121 gtctgtaggt atgggctttt tcctgtgcct caaagccatt tctcaaaggt ggacgatagg
    12181 gcatctcaag tacatcaggc atatggcagg tcgcagaggc gtctaaaacc aagatttcca
    12241 taccgttttc tacaatatct aatacctcag ttgctaaata acccgcatta agcgcaatgg
    12301 cttcaccagg ctcgatatag atttcaagat tgtaagtttt tcggatacgc ttgatttctg
    12361 aaatcagcaa atccacatcg taaccttctc ttgtaatatg atgaccacca cccatattga
    12421 gccattttac ctcatgtaag tagggaccaa attgttcttc tactgctttc aaagttgttt
    12481 gtaaatcatc tgctccctgc tcgcaaaggg tatgaaaatg aagtccgtca accaaatcta
    12541 gcaaatcact cggaatcttg tctatagtaa ctccaaagcg agaacctggt gcacaagggt
    12601 catagagcgc gtgatctcct tgagttgaac actgagggtt gaggcgcaaa ccaacactga
    12661 caccagcctc tcgacaacga gccccatgct tacgcaactg tctctctgag ttaaagacga
    12721 tatggtctgt tatcccaagc aattcctcca agtctgcatc cttgaaagca ggcgcaaata
    12781 catggacttc accaggaaat tcttcccttg ccaatttggc ctcatagagt ccactagctg
    12841 tcgtacctga tagatactgg ctaatcaagg gataagtttt gtagagggaa tatgccttct
    12901 gggcaagcaa gaccttgcaa ccggcctctt cttgtacata ttgtagaatg cggcaattag
    12961 cttctaactt ggccaagtca ataacatagg ctggtgttgg tacttgttct aacttcatta
    13021 gtccaccatt tgtggatttt caaccacaac ccatggcaaa ccatactcat tcaaagcttc
    13081 catgaatgga tctggatcta actcctcaag gttatacact ccagcttgtt tccaagttcc
    13141 gttcatgact aattttgtcc caatcatggc tggaactcct gtcgtataag aaatagcttg
    13201 cgaaccaacc tctgcgtaac attcctgatg gtcgcagaca ttgtagatat agatagtctt
    13261 tttgacaccg tctttgacac ctgtaaagat acatccaata ttggttttac ctacagtacg
    13321 tgggccaagg ctagcaggat ctggaagcaa ggctttcaaa aattgaattg gaacaatttc
    13381 ttggccgtta aagttaatgg tatccgtacg aaggagtcca acattttcaa gacatttcat
    13441 gtgcgtcaag taagattgac caaaagtcat aaagaaacga atgcgtttga cacctggaat
    13501 gttcttggcc aatgattcga tttcttcatg gtgaaggaga tacatatctt tttgtccaac
    13561 ttgagggaaa tcatactcac gcttgataga catagcttcg acttcgaccc atttcccatc
    13621 ttcccagtaa gaacccggcg cagaaacctc acggagatta atttctggat taaagttggt
    13681 tgcaaatgga taaccgtggt caccgccatt acagtctaaa atgtcgatat aatggatttc
    13741 atcaaaatag tgtttgaggg cataagctga aaagacacta gttacacctg ggtcaaaacc
    13801 agaaccaaga agagcagtca agcctgcttc tttgaatttc tcttgataag cccactgcca
    13861 tgagtagtca aagtaggctg taaaaccaag ttccttacaa cgtttttcgt agatagcacg
    13921 ccactcaggg tcttctgtgt cttctgcttc gtagttggct gtatcgatat agtgaacacc
    13981 tgttgccaaa caagcatcca taatggttaa atcttgataa ggcagagcta cattcaaaac
    14041 agcttctggt ttgtagcttt caatcaaggt aatgacctct tctaccttgt cagcatcaag
    14101 tgctgcagtt tcaattttag tacttgtttt gccttctagc ttcgctttca agtcatcgca
    14161 ttttgacttg gtacggctag caatcataat ctctgtaaac gtttcgctat cttgacaaat
    14221 ctttgaaata gcaacttggg caacgccccc acaaccaata actagtaaac gactcatttt
    14281 tttccttcct cttcttctaa aatgtcctca acatacttgg gcaacataaa ggctcccacg
    14341 tgtaagtttg cagtgtagta ttctgtgaaa agctggcgtt ttttccagcc ttccttgtca
    14401 aaatctttga cagggtggta ttttttcgat gcaaatccaa acaaccaata gccagctggg
    14461 ctagttggaa tatgggcctg ataaacccga ctgattggaa aggcttgatt gaccttgcgg
    14521 tgcatgcttc ggcaggccga ctcatcctcg tcaaagaagg gactcccatg ctggtaaatc
    14581 atgatgccgt cttccttcag agctcgataa ctattgccgt agaattcctt ggtaaagagt
    14641 ccttccgtat ggccaaatgg atctgtcgca tcgttgatga taatatcgta atcatcttcg
    14701 cagtttcgca aaaagcgtag cccattttgg tagtaaatgg taacacgagg atcatctagc
    14761 cctgcagcaa agtctgggaa atactcacga cagacctcga ccaacatctc atccggttcc
    14821 acgatatcga tttgttccag ttcaggatag agtgttaata cttgggcaac accgccgtca
    14881 ccacccccaa taaccaatac tttctttgga tttgggtgga cagccatggg aacgtgaacg
    14941 gtcatttcat tgtagacgaa atcatccgca tctgagaaca agacatggcc atttaaaatc
    15001 agtattttcc caaaagctgg cgtatccaag acttcgatat cctgccattc actttttcca
    15061 gcgtaaagtt gcttggctgt tctcagagac aatttgacat ctggagtatg aacttcagaa
    15121 aaccataaat ccatctattt ctccttatat tata
//
LOCUS       DAVBGY010000047        15048 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVBGY010000047 DAVBGY010000000
VERSION     DAVBGY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15048
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.047"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(67..615)
                     /locus_tag="VQ620_001533"
     CDS             complement(67..615)
                     /locus_tag="VQ620_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HET2764130.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(630..1478)
                     /locus_tag="VQ620_001534"
     CDS             complement(630..1478)
                     /locus_tag="VQ620_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HET2764131.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            1666..3015
                     /gene="trkA"
                     /locus_tag="VQ620_001535"
     CDS             1666..3015
                     /gene="trkA"
                     /locus_tag="VQ620_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HET2764132.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            3019..4458
                     /locus_tag="VQ620_001536"
     CDS             3019..4458
                     /locus_tag="VQ620_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET2764133.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVVVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(4584..6263)
                     /locus_tag="VQ620_001537"
     CDS             complement(4584..6263)
                     /locus_tag="VQ620_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2764134.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSTFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QSISFVFLSLILVVDILIYLPFCRAYDRQLLMKEDVASSNDIILEEETSEIMPGELDE
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKSLQFVLEHYQAV"
     gene            complement(6333..7745)
                     /gene="lacG"
                     /locus_tag="VQ620_001538"
     CDS             complement(6333..7745)
                     /gene="lacG"
                     /locus_tag="VQ620_001538"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET2764135.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGINMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKYNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSDDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            complement(7755..8099)
                     /locus_tag="VQ620_001539"
     CDS             complement(7755..8099)
                     /locus_tag="VQ620_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2764136.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            complement(8267..10153)
                     /locus_tag="VQ620_001540"
     CDS             complement(8267..10153)
                     /locus_tag="VQ620_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HET2764137.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYMRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGQMLWKKTRSYEELIEEYFFALYGENWQSVVEYLEKL
                     SIYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            complement(10155..11477)
                     /locus_tag="VQ620_001541"
     CDS             complement(10155..11477)
                     /locus_tag="VQ620_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2764138.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEAFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            11645..12868
                     /locus_tag="VQ620_001542"
     CDS             11645..12868
                     /locus_tag="VQ620_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET2764139.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            13167..13337
                     /locus_tag="VQ620_001543"
     CDS             13167..13337
                     /locus_tag="VQ620_001543"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2764140.1"
                     /translation="MTKQIPKFTKDTAQLYTCKWLLYNKVTKMYDHTIVNHSVREYIT
                     DSISTNTIEEAE"
     gene            13949..14233
                     /locus_tag="VQ620_001544"
     CDS             13949..14233
                     /locus_tag="VQ620_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764141.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            14226..14464
                     /locus_tag="VQ620_001545"
                     /pseudo
     CDS             14226..14464
                     /locus_tag="VQ620_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14560..14778
                     /locus_tag="VQ620_001546"
     CDS             14560..14778
                     /locus_tag="VQ620_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764142.1"
                     /translation="MMSIREQDLKDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
BASE COUNT         4944 a         3111 c         2406 g         4587 t
ORIGIN      
        1 ttcaaaacac tgttttgagc aacctgcggc taacttccta gtttgctctt tgattttcat
       61 tgagtattag tcctttttca aacttcctgc acgagtttgg gttcctgcat aggcaagtaa
      121 gagaagagtt cctgcaatag ctacagatac accattggca attcccgcaa caattccttg
      181 tgcaaatact ttttctgccg cttcttgata aatcacaaca tctccaagtg gtgccaagac
      241 accccaaaca agggcatttg caagtagttg aatgaggtta aaaataagaa tatctttcca
      301 gtcaaaaaca ccattgatca cgcgaacgta ctttctaaaa agtcccacaa ctaaaccaaa
      361 gagtccgcta gcgataatcc aagtccacca tagaccataa ccaacaagag agtccttgat
      421 tgcatgacca atcaacccaa caagcaaacc gataatcggt ccaaaaataa tagaaagtag
      481 cgcttgtacc gcatactgaa gctggatgct tgtatttgga acaggggttg gaatgttgat
      541 catcccgatg acgacaaaga gggcagcgcc aattccgaca gcaacaactt gtttaattgt
      601 aaatttgatt tccatactat tctcctattt tatccttcta ttttctttat ttcaatggtc
      661 caagatgaac cgacacctac attataggcc ttggcaaagg aaccttggtt gatagccaaa
      721 cctaaacgat agagagagtt gatgtaaagg atgggttgcc caattctcac atctgcaaat
      781 gatttgccat agacaacctg attttgatag accagcatat cagcatgata gatggtcact
      841 tcaaaacgat caccaaattc tggttccaac ttgtaaaatt cttcccgtgt gatagaggtc
      901 caaagcgaac cgaaacgcac atccagaata tcaatggctc ccttcaccag atgatcttct
      961 atgatggtcg ctacgactgg aagctctaca atctgttcca cactgagctc tggccctact
     1021 tcctcaaaag taatgtgacc actggccagt ttagcaccag tataggcata gacatcacga
     1081 ccgtggaagg tataagaatg ctctgtgttt tgacgcctat tggccacctc agaaatctca
     1141 cgaatggcta caatgccaac gtgtttcttg ataaaggaaa gcgtcccatt atctggcgtg
     1201 acaatgtatt gattttttgc agtcttggca actacactct tacgtttcga accgacacct
     1261 ggatcgacaa ccgatacaaa cgtcgttccc tcaggccagt aatccaccgt ctgaaagaga
     1321 cgatagctcc cctcaaaaat attataaggc gtgatatcgt gcgtcaagtg atggattttt
     1381 aaggttggag actcttctaa agccactcca atcatagccg ataccgcacc atcaaccaga
     1441 ccaaagtctg attgtaatac cagtaaatta ttattcattt tatctccttg tatatccttt
     1501 atcataccat atttcagaga aagtcgctaa tagctatttc aattggttag ggtatagttt
     1561 tatcttatag cgaattttca ctaaaagctc ttgttattag ctagtaggtg cattttttct
     1621 tgaaaaaggg tatgatagtt acatcatgaa aaagtaggtt ttattatgaa aattatcctt
     1681 gtcggagggg gaaaagttgg ttttgccctc tgtcgctcct tggttgcaga aaagcatgat
     1741 gttttgctga ttgagcaaga cgaagctgtt ctcaatcata ttgtcagtcg ctttgatatc
     1801 attggtatcc ttggtaacgg ggccgatttt gccattcttg agcaagccag cgtccaagat
     1861 tgtgatatct ttatcgccct gactgagcac gatgaagtca acatgattgc agcagttcta
     1921 gccaagaaaa tgggagctaa agaaactatc gttcgggtgc ggaaccctga atattctaac
     1981 tcttatttca aggaaaagaa tattctcggt ttttctctta tcgttaatcc tgagctcttg
     2041 gctgcccgtg ctatcgcgaa tatcattgac ttccccaacg ccctgtctgt cgaacgcttt
     2101 gctggtggac gcgttagcct catggaattt gtcgtcaagt ccaccagcgg tctttgccaa
     2161 atgcccattt ctgattttcg taaaaaattt ggtaatgtca ttgtctgtgc gatagagagg
     2221 gatcatcaaa ttatcattcc aagcggtgac atgactgtac aggataaaga tagaatcttt
     2281 gtcactggta accgtgtcga tatgatactc ttccataatt attttaaatc acgcgccgtg
     2341 aagagccttc tcatcgttgg ggcaggtaga attgcctatt atctacttgg tattctcaaa
     2401 gatagtcgta tcgatacaaa agtcattgaa atcaatcctg aaatcgccag cttctttagc
     2461 gagaaattcc caaatctcta catcgttcaa ggagatggaa ccgcaaaaga tatcctgctg
     2521 gaagaaagtg ctcaacacta tgatgccgtt gcgactctaa caggagtcga tgaggaaaat
     2581 ctgattacat ctatgttcct tgacagggta ggtgtacaga aaaatattac taaggtcaat
     2641 cgtaccagtc tcctcgagat tatcaatgcg cctgattttt caagtatcat cacacctaaa
     2701 agcatcgctg tagatacgat tatgcacttt attcgtggtc gagttaatgc ccagtattca
     2761 gaccttcaag ccatgcacca tctagccaat ggccaaatcg aaaccctgca attccatatc
     2821 aaggaagcca ataaaatgac tgccaaacct ctttctcaac tgaaattgaa aaaaggggtt
     2881 cttattgcag ccattattcg aaagggcaag actattttcc caactgggga ggatatgttg
     2941 gaagttggag acaagctcct agtaacaacc ttgttgccaa acatcaccaa gatttatgac
     3001 ttgattgcga ggtaagaaat gaataaaagt atgattcgtt acctcctttc aaagttactt
     3061 ttgattgaag ctgttctcct cttggttcct gtggctatcg ctgtctatta ccgtgaatcg
     3121 agccaagtct ttacagccct cttttcgacc atagggattc tcgtattgtt aggcggttca
     3181 gggagtttac agaagccaaa aaatcaacgg atttatgcca aggagggagt cttgatcgtt
     3241 gccctctgtt ggatcctttg gtctttcttt ggtggtctcc cctttgtctt ttctgggcaa
     3301 atccccagcc ttattgatgc cttttttgag attagttccg gctttacgac tactggagca
     3361 agtattttga acgacgtttc agttctcagt cgttccctcc tcttctggcg aagttttacc
     3421 cacttgattg gagggatggg agtgcttgtt tttgcacttg ctattatgga caatgccaaa
     3481 aatagccacc tagaggtgat gaaggctgag gttccaggtc ctgtttttgg caaggttgta
     3541 tccaaactaa aaaacactgc ccagattctc tatctccttt atctagctct cttctccctc
     3601 tttgtcatca tctattatct agctggtatg cctctctacg atagttttgt cattgctatg
     3661 gggacagcgg gaactggagg ctttaccgtc tataacgacg gaattgccca ctatggcagc
     3721 tcactgatta cctatctggt cagtatcgga gttctggttt ttggagtaaa tttcaacctc
     3781 tactactacc tcatgctccg tcgcatcaag gccttctttg gagatgaaga gcttagggct
     3841 tacttggtca ttgtagtggt ttctacaggc ttgattagcc tcaacaccct ctacctctac
     3901 ccagggtttt caaagagctt tgaaatgacc ttcttccagg tttccaacat cattacaaca
     3961 actggttttg gatatggaga tattaccaac tggcccctct tctcccagtt tatcctcctt
     4021 ttcctcatgg caatcggtgg ctctgctgga tcaactgcag gtggactcaa gattattcga
     4081 ggcctcatcc tttcaaaaat tgccaaaaac caaattttgt caatcctatc gccccaccgt
     4141 gttttgaccc tccatgttaa taaaacggtg attgacaaag atacccagca taagattctc
     4201 aagtactttg tcatctatgc tatgattttg ctagcgctta tctttattgt cagcctagat
     4261 agcaatgatt ttctggtcgt gaccagcgct gtctttagct gtttcaataa tatcgggcct
     4321 attctaggaa ccacttctag tttctcaatc tttagtccca tctcaaaaat tctcctatcc
     4381 tttgcaatga ttgcagggcg cttggagatt tacccaatcc tccttctctt tatgaagaga
     4441 acttggtcta agagataaaa tacaaaatcc ctctttttac aattgtgaaa gagggattct
     4501 tatattaaaa aagggggaac gtttttaaat ccaagcctat ctgtaagcaa aatccttact
     4561 caaataaaag atggaaaaac ttactacaca gcttggtaat gctccaatac aaattgtaag
     4621 gatttacttg gactctttgt taaatgaata tattccattc ctctggtagc aactatctga
     4681 atacctaatc tttctgcatc caccttcatc tctctataat aactccgaac ttgtggagcc
     4741 agaataatta aatcataaac acccataata tcataatgag ctccgtacgc tcctgaattc
     4801 gcaatcactc taacttctgc taattgagcg ccctcgttaa ttgcattggc taattgcgca
     4861 cttgttccag accctgcaca aagaaccagt actttcaact ccttactttt tatttcatct
     4921 aactcaccag gcattatttc acttgtctcc tcctctaaaa taatatcatt tgagcttgca
     4981 acatcttctt tcatcagtaa ctgtctatca tacgctctac agaatggcaa ataaatcaat
     5041 atgtcgacaa ctaaaatcaa agataaaaat acaaaggaga tagattgaaa attcgttcca
     5101 attaacaatc ccaagggacc aggaaaggcc caaggtaact ggatataaaa tccattcatt
     5161 ccaaagaaat caataaagac ctttcctaga aatacattca ctggtggagt catcaaaaaa
     5221 ggtacaaaaa gatagggatt caaaataact ggcataccaa atagaagagg ttcattcacc
     5281 gcaaataaaa caggagtaat tgtagcttta cctaccgatt ttaattgttt agaccgcata
     5341 aagaaaatca aaataaatgg tacaacaaag gtagcccccg ttcctccgat agcagcgata
     5401 taatttccga aattaggcgt taaagaatga taaggaaact gcccatttgc aagaagttga
     5461 gcattctctt ccatattgct cagttgtaaa gctgtaacag caggtaagac aatagatggt
     5521 ccatgaattc caacaaacca aagcaaagcg cacataaacc aaatcaacat catagcagga
     5581 tatgattctg cccccttaaa aataggagtc aatagttgtt gaaatacttg ggcaaaagga
     5641 acatctaaac taaaccgaga tacaatatct aacaaaccac taataagtaa aacaaaagaa
     5701 aaaggaaaaa tatctctaaa agcttgtgat atagccccag gaacttcctt aggtaaatga
     5761 atagtaatgt ctcgcttaat acagaatcgg taaacattta cagtaataaa ggtactgaca
     5821 aacgaagtta tcaatccttg agatcctaaa tagtttgtcg acaaacttgt agatcccgtt
     5881 ttctcatcaa ctacaagcgt tacagttaat acaagatagc cacatatggc cgccaacatt
     5941 gcagaagtat catttattac ctttccagga ggcatttttc tattaacatt tccagccaat
     6001 gactttgcaa ctgtccctga aacaataaca cccaaaactc ccatggttag catatacact
     6061 ttcctcatcc aggcaatagc tggactcggt aactctactc cgaaattttt aggaatcata
     6121 ataataatca tcaaaaagct tgaaaacata attaaaggca tagtagcaat aaatccatct
     6181 ttaatagcca tcatataatt attagtagaa attttttcaa atttcccttg ttgcttttca
     6241 atggcagcga ctaacttatc cataaaaact cctcctgtaa aaaatttaag ctcatagtta
     6301 aaatatatta aacatcagtc aaatcgctaa ccttaattcc gttttaatcc ttttacccaa
     6361 agagcacttt tcttaatata tctcttctgt gtttcataat caacaaagaa aagaccatat
     6421 cgcttattgt agccattcgc ccaagaaaat tgatcttgca aagaccatat aaaatagcct
     6481 tgaatattga ctcccctatc tcgagcctct aaaactttgt gtaaatgttg ctcaatataa
     6541 cggattcttt tactgtcatc aataatatca ttctcgccct ctggcttaac ctctttaagg
     6601 gctgtaccat tttcagttaa ataaatgacc ggatgttgag gatattcttc tttgatacga
     6661 agcaacatat caaataaccc ttgaggataa atattccaat cccaatctgt tgtcggaata
     6721 ccaggctttt ttacaaattc acctagagca tttaatttaa aagaagactg tccttttatt
     6781 cctgttgaat taaattcttg tctattttca cctttgtatt cacgaataaa ttctgaacga
     6841 taataattca tcccaaacat cgtattaaga ggtgcagctc ttttcataat ttctaaatca
     6901 ccatcttcaa taataaagct agaattatta gcttccaata tttgattcaa gtgaaaaagc
     6961 gtgtcatcac tgtagtagcc caaaaaagtt ccatctaata gaaatttatt attataaaca
     7021 tcataccgtt tagctgccaa aatattttct ttttgcccat caataggata gcctggcttt
     7081 aaagcatgaa tacaacctac ctttccctca atccctaatt gatgaaattc gaggactgca
     7141 agagcatgcg ccaacaacat gttatgattt gcttgaattg cttcagataa ttgaaaatgg
     7201 tgattcggag gaaactgacc tcctatgtat tggcctgcag caagagacat cagttcattg
     7261 attgtaaacc aatgcttgac ttctgtaaat tcttggaaac aaaagcgagc atatcgtatg
     7321 aaacgatcaa tattctctct attcaaccaa tcccctgttt ctaacatttt ctgaggcgaa
     7381 tcaaaatgat gtaaagaaac aaacggaatc acattatatt ttaagcagga ctgaaagact
     7441 ctatggtaat aacgaacagc caatatattc ggctccccat ctatatcagg aaatatacga
     7501 acccaagaaa tagataaacg caaagcctgc aaaccatgtt ctgccgccaa agctatatcc
     7561 tcttcgtaac gataataaaa atcactagct ggatctggta agaacggact attttcttgc
     7621 aaataaacat cccacatatt tattcctttg ccatctaccc tagttgcacc ctctacttga
     7681 taagcagcgg tagcagctcc taataaaaac tccttcggta aggctttaac ctgtaaattt
     7741 tccatgacta atcctcattg caaaccaata tgttcttcaa tctttcgaat tcgttcatat
     7801 tcatcaataa aaaactttac ctgatcatac aatagcatcg ttgtcatcaa agtatcttga
     7861 ccatgaatca tgataaaact cacttcaaaa ttatctccag atgcctcctc cgctaataaa
     7921 ttagtctgtt ctcgatgggc agacactatt gagtcgtttg cagactcaac caattctctt
     7981 gcctgttcaa aatatccatc tctcgcaaac gccaaagcat ccatcaaatc tgaccttgca
     8041 tctcctgcgt aagcgacaat tgtaaaacca agcatattta tttcttcttt tgatttcatc
     8101 tttcttcctc ctcaatcttg aaccatcttt tacacatcat tattaaaatg acttcgctac
     8161 gatacaaata gaaagtcgac cctaaatatc ttaaaaatcc ttaaatttca taatatttag
     8221 gatcaatctt ctctgaatac cttggttcat tttctagact tatgatttat aagtggaaac
     8281 cagcgtaatt ttttgctact tcaattacac gataaacatc caaattagat tggaatagca
     8341 gttcgtgccc acggatataa ttcaacacat ttctccattc atcttgagct tgccttgttt
     8401 ttccagttgc ttgaagatat aaagccttcg ccatcttaac aacatattca cgatgataac
     8461 tgagctgttt ccattcatcc ttttgactat ttaataactt agaaatattt tcctcaataa
     8521 ttggtaaaaa attatcagct agattgtaag ctatataata atgattcgct aaaacatcat
     8581 tttgacgact gccaattgca ttaaaatagt cacaagagga ataaatggat aatttttcta
     8641 aatattcaac aacagactgc caattttccc catacaaagc aaaaaagtat tcttcaatca
     8701 attcttcata acttcttgtc ttcttccaga gcatttgccc catgacataa ttaggaaaat
     8761 tatgagggaa tccggcacgt aattcttgac acgaaatgta cccattcaaa tgtaggtttg
     8821 aaagataaga tacatctctg taaatagttt gactaatttt catatagcct aaatcgccat
     8881 aatgagcacg ccctaaagga tagtcatata cgaaactatc tcctttaaat gttttttgcc
     8941 actcaaaaag ataagataaa ttttcctcaa gagaattcgg aagtataatt ttattacgca
     9001 tataaggttt aggcgtaggt atggaattgt caaaatctac atctgcataa ctcatttcaa
     9061 atgttcttgt aatcggtgca aacatcatgg taaagcgttc aggattatct aatttttctt
     9121 tctgaggcgc ccataacaac tcatggtaaa gcagaaaaca aatctttgta tctaatccct
     9181 cactcgttaa agccctatcc aattgattga gaatacgaat atactgatcc gaaaccaatt
     9241 cttgtctaca gttttcgcat tcacaaatat tattacgagc atccgacaac catacatgta
     9301 agtagttaac atcaggtctt ttcttggcat aatccttgat aatttctacc atcttatcag
     9361 ctacatctgg atttgaaaaa tccaggctgg ttaaaatcgg agccgtatta aacaattctc
     9421 gtttcccgtt tatttcagcg acatagggtt tcttctcctc tgaaatacta agacctgatt
     9481 cccagccaaa ttttgaagag taacctaaaa cctcacctgt ccatccatga ccaacacgat
     9541 gatgaataag acctcttttt tgcaattctt tatccaacct atcactcaat tcttgtacta
     9601 attcatttga gaatttttct ttatttaggt atggattaaa ctcatgttca taccaacgtt
     9661 tcaaaaaaga gtaaggattt tcaaactgga tgaaaaaact gttcatccca atcttaggta
     9721 gccaatcaat gaaatctagt atattttcaa atgaatccgc tccctctata catactcccc
     9781 tatgatagta actggctgtt tcatctatag atagctgttt atctaaaaaa tcttcaaatc
     9841 gtaactctgg aacaaagtca tgtctgcgcc ctggtctagt atacacaacc ccaaattcat
     9901 gaaacattcg gtaaattcca ataagtaaac ctacaattgt attagactca attctcccct
     9961 tacccttgtc taatgagata agaacatggt cttttctgtc ctctttattg actaatccta
    10021 caaaaatatc cgcttccttc tcctcgacaa gctctatgtt agtcttaaag acccgattca
    10081 tatagtaact taattcctct aaggcaaagc tagaataagt atttttctgt tgatcaaata
    10141 ctctgataac catcttattt ccctccaaca gtagcttttt ctttttcaag attgcgttta
    10201 tctgcaattt tgaaaaatgg ataataaatt gctacagaaa ccaaaatcaa aacaacttgt
    10261 aatagagctc ctcgccaact agctcctgtc gcaaggaagc ctcctatgat cggtggcatt
    10321 gtccacggaa ggattacacc tgttgtatag ggtactaatc ctacagccat tgatacatag
    10381 gtaatcaagg cattgattgt aggagtagca ataaacggaa tcagcataat cggatttaaa
    10441 actgttggaa acgtaaatag aatagctgta ttgatattaa aaatagacgg tataatagct
    10501 agcttaccta atgttttatt cgcacgactc ttactaaata ggaagagaca aatcgcaaga
    10561 ccaatagtcg ctccaccgcc accaataaat acaaataaat ctttaaacgg taatgtaata
    10621 atgtgttgga gtgtttgtcc tgcagccaca gcttcttggt tttctgtagt aaattgtaac
    10681 caaactgggt ctacaaaagc atttaaaact tgtcccccat taactccaca gaaccaaaag
    10741 aatgagttta caataacaca tagaatcata cctggaagcg ttcctgcaat taacttaagc
    10801 ggtgttccaa caattgcacc taggagtccg ttgagacctc ctgcaactcc tgccgcttca
    10861 agacctttta agaccagagc ccacaaaaca aaagtagtaa agccaggtaa aagagctgaa
    10921 aatgatttac ttaccacatc tgggacacca cttggcattt ttatcgtaat tccgcgctgg
    10981 ataaacatac gataaatctc tgctgtaacc aatccaatag taatcgccat aaacaaagaa
    11041 gatgcattca ggctagaaaa tggtattgcg ccgccaaata actgcttgac ctgctcccca
    11101 tttttatcat aaaccatact cgaaaaacga ggtgccatca atacaaaact ggataaggct
    11161 atgatccctg ccgacggacc atctgtacca tatccttccg acaacctgta ggcaataccg
    11221 aaacaagcga ccaaggagat aatcgctact gttgactgat tcatagttgt caagatactt
    11281 agcaatccaa cactattcag ccaatttgta aaagcctctt gaggccaact gattagaata
    11341 agaaataaag agccgactaa aatcaaaggc attgccatca taaatccttc acgaatagct
    11401 ttcaaatgcc gttggttgcc aactttttca ccaaacggag cgacttttgt ttctaaaaac
    11461 gctaaaatat tgttcatcac cggaactcat ttttttattt tttataaatt aaatatacca
    11521 ctaacagaaa gcgttgtcaa tatatttttt gttgttttta caatattttt ttctttgtat
    11581 agaataataa ttttaattga ttttttctta tgtttaatat aaaataaacc aaaggaggaa
    11641 acaaatgatt acaaaacaat cagccttttt acaaaataga gccactatac tggagtttct
    11701 atatcgaaat ccagcaacat cccgtacaga tattgttaat gaaacaggac ttactccagc
    11761 aacaacgact aacatcataa aagagctaag cgaacagtcc ctcatatacg aaactggtga
    11821 tgaatttagc gagttttccg gatctggaag acgtagaaaa actatttcta tcacagataa
    11881 tattccgtat gttgttggag gtattgaaat aaacgtccta ggtatctttc tcagtctatg
    11941 tgacttacaa gggaaaactc ttttcgagac agaaattttg aatgaagatt atcctatttc
    12001 agaaatcaat tccaccatta ccaatatgat aaaaacagct atagagtacg tccctttgga
    12061 aacaaaatta cttggatttg gcttatcaat acctggacat tataacaaag actccggaag
    12121 tatcattaca aacaacccca tatgggaatc ttttaattta ttaaatgtaa ttaaaagatt
    12181 caattttcct tttattgtaa aaaataatat cgattgtatg gctataggac aatacctttt
    12241 taatccacac aatacccccg ataactttat tttcctacac gctggattag gtatttacac
    12301 ttcctttttc acaaaagaaa aaataggagc ctctaaaaat ccttatatcg gagaaattgg
    12361 acacaccatt gtcgaattga atgggcaata ttgtgaatgc ggaaaaaaag gttgtttaca
    12421 aacatatatt tcggatgctt ggttaatcaa acatgcccaa ttattattta aaaattctca
    12481 actaactgta ctaaaaagcc ttgtaaagac tgaaaaagac attcatttag ataccctttt
    12541 aacagcttac aacttaggcg actctgcttt acgccaacaa attgataaag gagtcaattt
    12601 attggccact tctattgcaa atcttctcct catcaatcct gctgataaaa tctatatcaa
    12661 cagtcaattg cttaattatc aacctttcac tcatgaagtc agggataaaa tccaagatca
    12721 gctccacttc gttcccttta ctcgtaatat agaaattgaa attttacctt acaacaaaca
    12781 tcgtggaagt ataggagctt gtgcattagc tatcgttgct tttttcatag agcatagcaa
    12841 tgtattacaa gatattattt caccttaata tatattagaa atctatagac ctgtttaaat
    12901 caactataac ctgtagtaaa tatctcgtat ttagacaata tgaaaacaag acgacttcca
    12961 tataggaaac cgccttctcg ctatgctgca tgaaactgta aaaatagatg aaattgatag
    13021 cggtagtcag atcaagaaac atcgtaactc taaaaagtca caatatataa gttcatcctc
    13081 ggaaaaatat cattctaatt attgaaatgc ctacatgaaa agaaacgtta aatgctcata
    13141 aaacaacgaa tacaggtatc aaaactatga caaaacaaat ccctaaattt actaaagaca
    13201 ctgctcaact ttacacctgt aaatggttgt tgtataataa agttacaaag atgtacgacc
    13261 acactattgt aaatcatagt gttcgcgaat atattactga tagcatttct acaaatacaa
    13321 ttgaagaggc tgaataggct ttaaacgaaa tatgtcagtg ttttggcatt cctagccttc
    13381 atatcattta aagaattgta tagataaaat tttttccaat acagacactc gtaacaactg
    13441 cttcattttt ctaccaacat atttaggaac aggataagat acaagaggat taatccatag
    13501 ctcagttcta taccaatcta agacaaataa gctaaaaaaa cgattgataa taagcaaata
    13561 gattccaaat tttctctatc tgctcatttt aataaacaat actagtgtaa ctatccttcc
    13621 agtcagaagc ttgtcaaatc acaccgaaaa ttcttctaaa atttatctcg ttaggcaatc
    13681 aagcaaaaac tcgacgatag tacaaacatt atcatacaga attgacttcc taaattatat
    13741 actttagtaa ggttttcgga taagaaaaaa ggttcatttt acatttctaa acattctttt
    13801 ctaagatgaa aaacagaatt tttcgattgt aatttaaagc aacaagaaga ttttcagtat
    13861 catcctatag atacgagcta attaagaaaa actacatttt tgaatataaa ctacaataat
    13921 ataaactaaa ttccatagga ggaagacaat ggattggtac gattatatga tacaggcatc
    13981 caaacaatca caattcaacg caagccattg gtttcgctat ttgcgaaaag ttatttttga
    14041 agactattct tatttaacaa accaagatgt agaaaagttg ctagactcca aagaactaac
    14101 ccgttttcaa aaaattagct tgaagtatgc ctttcaagag catactccaa ctcataaata
    14161 tgtgatttca ttaaataaac ctgctaagtt aaccaatgtt caaaaattga tggagaaata
    14221 caaacatgga taaaatgaaa ccggtcttcc aagccctaaa taagaaatta attcaggaaa
    14281 atctgacttt aacaattatg gttatgtctt agaatatcat ggtttacgtg ccacacaaga
    14341 tgttgatgct ttttatgacc aaaatcagaa aatcaatgaa attattgctc gtgtaggaaa
    14401 acagtttaat cttaacatcc acgaagagtt atggctcaac aatcatgttg caaagcaaat
    14461 atgaacaaac aacctccctt aagtttatgc gaatccttgt actcatttga gaatctgacc
    14521 gttttagtag tccctattga gtatgtgtta ggaatgaaga tgatgagtat cagagagcag
    14581 gatttgaaag atattggtgc aattattaaa tacaaaaact ttcattcacc attcgatacc
    14641 tttaaatatt taaaagacat gggatttgat actattgacc tctctgttct attagaaggt
    14701 tttagttatg cttatggtat ggattggtta gaaaaatttt ttaaagaaaa tcaagataaa
    14761 cttcgcgaat tttattaaca gtatcaaaca agatgattgt caattagcca gtcgtcttgt
    14821 ttttatgatg gaatgaaata aaatcggaac aaatcactta gagaattcca gacaatttct
    14881 agcactgttt gagacgtttc agcggactat agcaaaatga aataagaata agacaaatcg
    14941 atcaggacag tcaaatcgat ttctaagaat gttttagaag taaaggtgta ctattctaat
    15001 ttcaatctac tatatttcag attcggtcca ctattccttc ccaaaaac
//
LOCUS       DAVBGY010000048        14463 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVBGY010000048 DAVBGY010000000
VERSION     DAVBGY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14463
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.048"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(122..1972)
                     /locus_tag="VQ620_001547"
     CDS             complement(122..1972)
                     /locus_tag="VQ620_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET2764143.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMVDFWGLSASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(2040..3179)
                     /locus_tag="VQ620_001548"
     CDS             complement(2040..3179)
                     /locus_tag="VQ620_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2764144.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKGKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDAEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGGLILKIDAHSKVTESFVMNNVAVIQQGEFVCGGPRP
                     TIVKGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLSLLTLLTLLKHK
                     NGFLIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKGTVIYLEK"
     gene            3237..4403
                     /locus_tag="VQ620_001549"
     CDS             3237..4403
                     /locus_tag="VQ620_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HET2764145.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TAMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIDILMTVALFTVDRADKIL"
     gene            4542..4868
                     /locus_tag="VQ620_001550"
     CDS             4542..4868
                     /locus_tag="VQ620_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET2764146.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            4855..5448
                     /locus_tag="VQ620_001551"
     CDS             4855..5448
                     /locus_tag="VQ620_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HET2764147.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEVAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            5441..6352
                     /locus_tag="VQ620_001552"
     CDS             5441..6352
                     /locus_tag="VQ620_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HET2764148.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            6415..7479
                     /locus_tag="VQ620_001553"
     CDS             6415..7479
                     /locus_tag="VQ620_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764149.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(7551..8411)
                     /locus_tag="VQ620_001554"
     CDS             complement(7551..8411)
                     /locus_tag="VQ620_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HET2764150.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            complement(8533..9519)
                     /locus_tag="VQ620_001555"
     CDS             complement(8533..9519)
                     /locus_tag="VQ620_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HET2764151.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(9791..11266)
                     /locus_tag="VQ620_001556"
     CDS             complement(9791..11266)
                     /locus_tag="VQ620_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HET2764152.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(11524..12447)
                     /locus_tag="VQ620_001557"
     CDS             complement(11524..12447)
                     /locus_tag="VQ620_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2764153.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(12461..13390)
                     /locus_tag="VQ620_001558"
     CDS             complement(12461..13390)
                     /locus_tag="VQ620_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2764154.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(13709..13930)
                     /locus_tag="VQ620_001559"
     CDS             complement(13709..13930)
                     /locus_tag="VQ620_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764155.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTEAEVIDNLKEVIAKLKANA"
     gene            complement(13884..14144)
                     /locus_tag="VQ620_001560"
     CDS             complement(13884..14144)
                     /locus_tag="VQ620_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764156.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKALADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            14224..14361
                     /locus_tag="VQ620_001561"
                     /pseudo
     CDS             14224..14361
                     /locus_tag="VQ620_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         4337 a         3099 c         2672 g         4355 t
ORIGIN      
        1 aaccatatac ggcaaggcga cgttgacgtg atttgaagag attttcgaag agtataagtc
       61 tctacataat aaaacgcata ttaccaaggt tatttaactt gataatatgc gtttttcttt
      121 tttattcaat gtgggttgtt tgattagcaa aggcgataat agcttgcttc aactcatctc
      181 cactgagagt gcggaactct ccaatctttt gattgatgga ttctaaaggc atgacattaa
      241 ccttaccaac gaaaatctta tccataactt gattatcaac gagttcggtt gataccaaga
      301 gttcttcgta gagtttttca cctgggcgga ttccaacttc aacgattgga atttcacttt
      361 cagtgtggcc acttagaagc accatcttct tggccaagtc ataaatcttg actggtttgc
      421 ccatatcaag gataaagact tccccatctt tggcataagc accagcatgg ataaccagac
      481 ggctagcttc tggaatggtc ataaagtaac gggtcatgcg gaagtctgtc accgttacag
      541 gcccaccttc agcaatctga cgttcaaaga ctggaatgac actaccacgg ctaccaagaa
      601 cattcccaaa acgaactgca cagtaggttg attggctacg ttggttaaag ccagtgacaa
      661 tcaactccgc cacgcgcttg gttgctccca taacatttgg tggattgact gccttatctg
      721 tcgaaatcat aaccatctta ggcaccttgg cttcatcaac ggctctagca acattgtaag
      781 ttccacggat attgtttttg aaggcttctt ttggattgcg ctccatcata ggaacatgct
      841 tgtgggctgc cgcatgataa acaatagcag gtttgtactg ctcaaagact tgcaacaaac
      901 gatcatagtc ttgaatgtcc gcaatcacag gtacataatc aatcccttgg aacttacgaa
      961 tcaattcatg ataaacaagg tagattgagt tttccccatg accgagcaag acaatgcgtt
     1021 caggattgaa gcgactaact tgacgacaga tttcagaacc gattgaacct ccagctcctg
     1081 tgactaagat ggtcttacct gtcagttctg cacccagacg cgattcgtca agacggattt
     1141 cctgacgacc caaaaggtcc gtaatatcaa ttttttggaa gccagtacct gcttggtgaa
     1201 ggccctgaac aacagtttca accttaggca tcttgtaaca tttgacaccc agcttattac
     1261 acatctgcaa gatacgctca tattctgacg gatccagcga cggaatcgca acgatgacac
     1321 gctcgatttg atggcgtttg gctaattcag gcagattgtc ataagagccc aaaacaggaa
     1381 taccaccaag tttttgaccc tttttcttag aatccttatc caaaataccg accagttcta
     1441 attcactggt tggatgttgg taactatcca taaagagagc cccaccatca ccggcaccaa
     1501 tcaagaaggt ccgacggtgt tctccatcac cactaccttt tttgcgtctg gagtagatta
     1561 actgccaagt aatccgtggc aataaaatca agaaggtact caacaagata aagagaatga
     1621 taaaacggat ggagaagagt ggcaagaagg cataacagat actatatgac aagacactgc
     1681 tagcagtcac accaaaaaag attttcatga aatccgtaat cttgctgtaa cgactaatac
     1741 tcgcgctcaa gccccaaaaa tcaaccatca attgatagaa caggaaggcc aaactcgtat
     1801 agataatgta gtcaacaggt gctggattaa tcagcccata aaataaaata taagatacaa
     1861 tgatggaaac caccatactg aaaatatcaa atattcccca gaaaacctgc ttttgttgtt
     1921 tatttaaaat ttccaccaga tcaatcacat aatctgtgag ttttttattc atagaaattt
     1981 actcctcctt aaaatagttc tactatattg ttattatagc acattttaat ttatttggct
     2041 tatttttcca aataaattac tgttcccttg tactccttct tccatttaaa tcctctaatt
     2101 aaacctacaa tcgtcccaag gccataagca aagtgaatgg aaaataaaag aaagggcatc
     2161 acaattagaa atccattttt atgttttaat aaagtcagca aagtgagtaa tgacagtaat
     2221 gataaaagta gaaaataggc acctaatagt aaagttatga atacgaatgt gatcggtaac
     2281 aatgctagac taaacacaag actcaaaaca aataaacaag gaacataatg aaataatgat
     2341 aaacacttaa actgaacatg acttgtcaag ccaatccaca aaccatttga atacttttga
     2401 tgcagcattt tcttgaatgt tggtcgaata tactgataag atagaatact tgggctatag
     2461 cggattttat aaccatgttc tcgaattcta taatgaatat cattatcttc agttcggcca
     2521 agttgctcat ttactaaacc aaccttctgg aaaacctctc gtttatacat cccatgaaaa
     2581 atagaagaaa catatctatc ctcggaacta tttcgataat tggcaatgct actgccaaac
     2641 atattttcct caacaagatg caaggtctct gcccattttc cttttccttt gacaatcgtc
     2701 ggtctaggcc ctccacagac aaattcacct tgttgaataa cagccacatt gttcattaca
     2761 aaactctcag taacttttga atgagcatca atttttaaaa taaggccccc tacagaatgc
     2821 ttaactccca ggttaaaacc actagcttga tttttcttag gattgttata caatctaatt
     2881 gagttaaact ctgcatcttc ttttataaat tgctgaatga tagctgtggt cccatctgtg
     2941 gacatagcat ttataaatag aatttcaata ttctctttag gataggtttg attttttaag
     3001 tcttcaatta gaccaggcag atatttttct tcattgtatg cactgattac aatggatacg
     3061 agaagacttt caacattttg agtcactact actctcctga attttccctt ccaccattct
     3121 atcataattt tcaagacttt tccattttta tcttgaaagc cagaaacctt acttgacatt
     3181 atagtgaaaa agccttaaaa taagctgtta ttaaaatcag ctagaagaga tattatatga
     3241 aaaagcaatc actctttttt gttccaggta ttatcctgat tggtgtttcc ttgcgaactc
     3301 cttttactgt tttacccatt attttgggaa atatttcgca aggtctggag gtagaagtta
     3361 gttcgcttgg tgtcttgacc agcctgcctc tccttatgtt taccctcttt tcaccatttt
     3421 ctacccgact ggctcagaaa atcggcttgg agcatctctt cacctacagc ctcttcttct
     3481 tgaccatcgg ctcacttatt cgactaatca atctgcccct gctctatcta ggaaccttga
     3541 tggttggggc aagtgtcgca gtcatcaatg tcctgcttcc tagtcttatc caagccaatc
     3601 aaccaaagaa aattggtttt ctgaccacct tatatgtaac gtctatgggg attgcaacgg
     3661 ctctggcttc ctatctagct gtgcccatta cacaagccag ttcttggaaa ggacttatcc
     3721 tcctcctcac gttactctgt ctagcaactt ttttggtctg gctcccaaat caccgctata
     3781 atcatagact agctccacaa accaaacaaa aaagtcaaat aaaggtcatg cgtaataaac
     3841 aggtttgggc aattattatc ttttcaggtt ttcaatcctt gatcttttac accgccatga
     3901 cctggttacc taccatgtct atccatgcag gtctatccag tcacgaagct ggcttgctga
     3961 cttctatctt atctctgatt agcattcctt tttcaatgac catcccaagc ctgacaacca
     4021 gtttatctac tcgcaaccgt cagctcatgc tcactctggt ttcactagct ggtgtggtcg
     4081 gcatttccat gctctttttc ccaatcaata atttcattta ctggcttgcc atccatctcc
     4141 tcatcggaac cgcaaccagc gccctcttcc cttatctcat ggtcaacttt tcactcaaga
     4201 caagcgcccc tgaaaagaca gcccaattgt ccggcctatc tcaaacagga ggctatatcc
     4261 tagcagcctt tgggccaacc ctctttggtt acagttttga cctattccac tcttgggtac
     4321 cagctgtagc tgccctcttg ctcatcgata tcctgatgac tgtggccctc tttacagtgg
     4381 acagagcgga taagatcctt taaacttcta gttttagcta gaagtttttt atatataaaa
     4441 attttacaca tttctcttga cagggctttc ttttagatgt acaatgtatg tgtagaaaaa
     4501 ttatatataa aaatcttaca cattagaaaa ggaggtttcc catgtacttt ccaacatcct
     4561 ctgccttgat tgaatttctc atcttggctg tactggagca gggtgattct tatggttatg
     4621 agattagcca aaccattaag ctgatcgcta atatcaaaga atccacactc tatcccattc
     4681 tcaaaaaatt ggaaggcaat agctttctga caacctattc tagagagttc caaggtcgca
     4741 tgcgcaaata ctactccttg acaaacggtg gtatagagca gctcttgacc ctaaaagatg
     4801 aatggacact ctatacagac accatcaatg gcatcataga agggagtatc cgccatgaca
     4861 agaactgaat acctgactca gctagaactc tatctcaaga aactacctga agctgaccgt
     4921 atcgaagcca tggactattt cagagagctc tttgacgatg ctggagtcga aggagaagaa
     4981 gaactcatcg ctagtttggg aactcccaaa gaagtggccc acgaagttct atccaatctt
     5041 ctcgataaaa aaatcaatga agcacccgct caaaaaaata accgacaaat tttacatatc
     5101 gccttgttag ccctccttgc agcacctatc ggcattcctc tgggaatcgc catcctcgtg
     5161 accctgttcg caatccttgt agccgctttg actgtcattc tggctttctt tgcagtttcc
     5221 atactgggta tcatcggcgg attcctattt ttagttgaaa gtttcactgt cctcgcccaa
     5281 gccaaatcag cctttatctt gatttttggt tctggtttac tggctatcgg tgcttcttcg
     5341 ctagttttac ttggcatttc ctatgtagct cgcttcttcg gtctactcat tgttcgtctg
     5401 gtacaatttg ttcttaaaaa aggaaagaga ggtaatcagc atgcgtaaat ggacaaaagg
     5461 atttctcatc tttggtgtgg tgactaccgt tatcggcttt atcctgcttt ttgtaggtat
     5521 ccaatctgac gggattaaga gcctactttc catgtccaaa gaacctgtct atgatagccg
     5581 tacggaaaag ctaacctttg gcaaggaagt cgaaaaccta gaaattactc tccaccaaca
     5641 cacgctcacc atcacagact ctttcgatga tcaaatccac atttcttacc atccatctct
     5701 ttctgctcac catgatctta tcaccaatca gaacgataga actctgagtc tcactgataa
     5761 gaaactgtct gaaactccgt ttctctcttc tggaattggt gggattcttc atatcgcaag
     5821 tagctactct agtcgttttg aagaagttat tctccgacta ccaaaaggga gaactctaaa
     5881 agggatcaac atctcagcca atcgcggaca aaccaccatc ataaatgcta gccttgaaaa
     5941 tgcgaccctc aatacaaaca gctatatcct ccgaattgaa ggaagtcgta tcaaaaacag
     6001 taaactcaca acgcccaata tcgttaatat ctttgataca gttcttacag atagtcagct
     6061 agagtcaaca gagaatcact tccacgctga aaatatccaa gtccatggta aggttgaact
     6121 gactgccaaa gattatctca gaatcatcct agaccagaaa gaaagccaac gaattaactg
     6181 ggacatctca agcaactatg gttctatctt ccaattcaca agagaaaagc ctgaatcaag
     6241 aggtacggaa ttaagcaacc cttacaaaac tgaaaaaacc gatgtcaagg atcaactcat
     6301 tgcgagatct gatgataata ttgatctaat atccacacca agcagacgtt gacaaaaacg
     6361 cttacatctg ctactatgga agcatattta tctaactaaa aaggaggttc taccatgaaa
     6421 caagaatggt ttgaaagtaa tgattttgta aaaacaacaa gcaagaacaa gcctgaagag
     6481 caagctcaag aggttgcaga caaggctgaa gaaacgatag ccgatctcga tacaccaatt
     6541 ggaaaaaata ctcaagtaga ggaggaagtc tctcaagctg aagtcgaact ggaaagccag
     6601 caagaagaga aaattgaaac tcctgaaggc ggtgaagcga gaacagaaat agaagaaaag
     6661 aaggcatcta attctactga agaagagcca gacctttcta aagaaacaga aaaagtcact
     6721 atagctgaag agagccaaga agctcttcct cagcaaaaag caaccacgaa agagccactt
     6781 cttatcagta aatctttaga aagtccttat atccccgacc aagctccaaa atctagggat
     6841 aaatggaaag agcaagtgct tgatttttgg tcttggctag tggaagcgat caaatctcct
     6901 acaagtaagt tggaaacaag tatcacacac agttacacag cctttctctt gctcattctg
     6961 ttttctgcat cttccttttt ctttagtatc tatcacatca aacatgctta ctatggacat
     7021 atagcaagca ttaacagtcg cttccctgag cagctagctc ctttaactct tttttctatc
     7081 atctctatcc tagtagcgac aacactcttc ttcttttcat tcctcttggg tagtttcgtt
     7141 gtgagacgat ttatccacca ggaaaaggac tggacgctag acaaggttct ccaacaatat
     7201 agtcaactct tggcaattcc aatcttcctc actgctattg ctagtttctt tgctttcttt
     7261 gatagcctac gatttacggc cctcttgtgt gtgattagca ttggaatcat tctgcttgcc
     7321 agtctccata tcattacaag accaagtcaa gcaagtgaaa ccgactcctt ctatcaatta
     7381 ttcttgtctg tccttgtgaa cggagttatt atcctcctct tctttgtagc tgaagtggca
     7441 ctgattggag attatcttcg tatcttggcc tttctttaaa attcaatcct gtcatccttg
     7501 gcaggatttt ttctatgtca aaaaagcaag tcgattgacc tgcttctgct ttacttttct
     7561 tgtgctaatg ttttaaaatc tttgtctaat atgggttgca cttctatttg attggcgtct
     7621 agttggtggt aaaaagatgg gggtaatacc tctaagaatt ttgcatagtc caagcgagca
     7681 atggcttcat agtcttctgg tttagagcca tctcctgtga tttgaaccaa gtcaatctcc
     7741 cactggactt ccttatccac caattgctca atataggctg caggaacaaa cggttctctc
     7801 ggtaaaactg tcttgactcc ttgagctaaa tggtaaacgc catgcacttt tccttctcca
     7861 tcctgataga aggaaactct tgcaaagtag gcatctgtct cttgaaccgc acgcacacgc
     7921 gcttcaaact gagccaagcc atctgcctct aaaaagcttt tcaagttctc acgactaaag
     7981 aaaataggat taaaaattcc ttggcaaatc gtaaaacgag ctacagggtc tgctagatag
     8041 gcttgaatac ttgcttggaa ataagcttca aaatcaaaac catctagccc ttcaatatca
     8101 gtccctgtat aagcaagcaa agcatctaaa aatgacttga taatctgcca gtctgtcttt
     8161 gttagtaggt caggaagatc gacacggtaa gccttttgac catcagcata actgacctta
     8221 aagagaaatt gtgattgccc cactatcgca cactcaatat actcgagacg attaagaggc
     8281 tgacgaagat agactacatc atagctatgt gactccaaac cgtctagcaa ggccaagatc
     8341 gacttggcat tcaaaatctc ttgttctcct aaaatgctct gtttatttgg aataaaaaat
     8401 gttttcgcca taactggacc cctttgaaat cgtttacata cagtatacct tattttcctg
     8461 aaagatacaa aaacaaactt tagatttttg agtacaaagc atagaaaaac agcccctgag
     8521 gactgtttga tcttatacag tagctgcctg atccaagctt tcaccgatag cggctaggcg
     8581 ctcaatcact tcagcttgtg tcaattcatt ttttgaaaca tagcggttac gtgggtgaac
     8641 acggcactcg tgtgagcatc cacgaaggta cttgtcttca ttttcttctg atgtcaagat
     8701 acgacggtta cagaagggat ttccacagtt gacataacgt tcacatggtg ttccatcaaa
     8761 ccagtctttc cctacgatgg ttgggttgac atggttgaca tcaacggcaa tacgctcgtc
     8821 aaagacgtac attttcccat cccaaagctc accttgaact tctgggtctt taccgtaagt
     8881 tgcgattcct ccgtgcaatt ggccgacatc tttgtagcct tcacggacca tccagcctga
     8941 gaatttctca cagcgaacgc cacctgtaca gtaaaccacg acacgcttgt ccatgaattt
     9001 ttccttgtta tcacggaccc attgtggtaa ctcacggaag ttgcgaatat ctgggcgaat
     9061 agccccacgg aaatgtccta ggtcgtactc ataatcgtta cgtgtgtcaa ggacaacggt
     9121 atctttatca agaagcgctt ctttgaactc ttttggagac aagtaagcac ctgttgtttc
     9181 aagtgggttg atgtcattgt caaagtcgtt gtcttccaaa ccaaggtgga caatttcttt
     9241 cttgtagcga acaaacatct tcttgaaggc ttgttcattt tcttcgtcaa tcttgaacca
     9301 gagttcttcc attcctggaa ggctgtgaac gtagtccatg tatttttgag ttgtttcata
     9361 gtcacctgaa actgttccgt taattccctc gtcagcgact aggatacggc ctttaaggcc
     9421 gattgattta cagaaagcca agtggtctgc agcaaattgc tctgcatttt caattggagt
     9481 ataaaggtag taaagtaaga cacgaatatc ttttgccata agatttgttc tcttttctat
     9541 tcttaaattt tctaaatttt ttatttatct acactagtat acctgcttga gaaaacgaat
     9601 gcaatttatt tgactggaaa ttgtagaggg ttctcgaaat ttcctgaatg gttaaaataa
     9661 ggacaagaga aaacatggat atctatatcc ttgtgccaaa aaaaccactg ccctccccag
     9721 accaacctga ggaaagcagt gattcttatt ttaggagtta ggaatgaata cacgaaatca
     9781 atttagctga ttattttttg tttttcaaga attcatcgta ttgtttttgc atttcgttca
     9841 atactttttc gtaggcacct tcagatttca atttttccat caattctgga atcgctttat
     9901 ctgggtctac agtaccagtg ttgatagctg tatcaaattg ttgcattgtg ttagcaatag
     9961 ctgagatttc agatttcaca ttgtcagtat tgaagataaa tccaagcgct ggagattctt
    10021 tagcttctgc caattctttc ttagaatttt cgatttgttg gtctgtaacg ttttcgttga
    10081 tgtaaaggat ccagttgtta ccagtgttcc atccacccat gtgagtgttt cctttgtagc
    10141 catcaagaac gcgaacacgg ttttctttac cttcaatttt ttcccagttc ttgccttctg
    10201 gaccgtaaac aagaccgttc aagagttctg gattcgtatt caagaggttc aagatttcca
    10261 ttgatttttc tttgttctta gagttgtttg agatgacaaa gttagcaact tgtgttgttt
    10321 ggtttttctt gatgaagtta gtaattggtt tgatttggat atctttgttg gcaacacgtg
    10381 aaagcaagct gttaccgtag tcagctggtc ctactgtttc ttcacgaacg aaccaagtat
    10441 cttgttgaag gtcaaaggaa gtatcgcttg ttgcgacgtc ttttggaatg tagccagctt
    10501 catagaattt gtgaagagtc ttcaagtgtt ctttgaaacg aggcacttcg taacggttta
    10561 caactttagt agtatcgcct tcaaggtcga taacgaatgg aagaccgttt gctactgggt
    10621 agtcaaagtt atcagatggg atgaaaactt taccaatagc aaatggtact acgtctggag
    10681 ctttttcttt gatttgtttc aagactggct caagagtttc gtaagaagta acacctgaaa
    10741 tatcgatacc atatttagca aggagagttc cgttgaaggc aaagttttga gatgatgcaa
    10801 cgttggctgc aactggaaca gcgtaaatct taccatttac agtattaccc ttgatgtaag
    10861 ctgggtcaag tgctttgtaa aggtctttac cttctttttt gtacaattct gtcaagtcag
    10921 cgtaagcacc tttttgagca tttacaatat agttatctgc aaaggcaata tcatagtttt
    10981 caccagatga tgtgataact gacattttct taccatagtc accccagcca aggtattgga
    11041 tatccaattt agcaccaact ttttcttcga tgattttgtt ggcatttgct aacaattcat
    11101 ccaagttatc tggtttgtca ccgatttggt acattttgat aacaggtttg tcacctgaat
    11161 cagcagcttt tttgctgtta cctgtcaaat ttccacaagc agcaagacct gcagccaaag
    11221 cgactacact agcagatgca aaagcatatt ttttccagtt tttcatgata aaaactcctt
    11281 tttttatttt taaacttata aacaatgtaa tgatcttata ctcaatgaaa atcaaagagc
    11341 aaactagaaa actagccgca ggctgctcaa agcactgctt tgaggttgta gataagactg
    11401 acgaagtcag ttacatatat ctacggcaag gcgacgttga cgcggtttga atttgatttt
    11461 cgaagagtat taacttcaca caagggaagt tgggaactga gaaatgttct ttttcaataa
    11521 gcactattct ttcacaccac cgatagtcaa accttttaca aagtagcgtt ggaagaatgg
    11581 atataaaatc gcgattggaa gggttgcaac cacaaccatg gccatacgac ctgtttcttt
    11641 cggtagagca actcccagtt gaccagtcaa gccgaccgct ttggcaatgt agtccatatt
    11701 ttgttggatt tgcatgagca aatattgcaa tggatacaag ttatcactct tgatgtaaag
    11761 aagggcgttg aaccagtcat tccagaaacc aagagctgtc aaaagcgtga tggttgcgat
    11821 acctggtagt gacaatggca aacagatttg gaagaaaatc cgggcctcac tggcaccatc
    11881 gatacgagcc gattcgagaa tggcttctgg aatggtcttc ttgaagaagg aacgcatcaa
    11941 gatgatattg aatggtgaga gaagcattgg aacaatcaag gcccaaacag tgtcaccaag
    12001 ctgaagtaca cgggtcacca tgatataacc tggtaccaaa ccagcgttga acaacatact
    12061 gagaaggacg aagatggtaa aaaatctgcg atacttaaag gttgtccgtg aaatagcgta
    12121 ggcataggtt gttgtgataa agacatttgt caatgtccca actacggtta caaagacaga
    12181 gatgaagagg gcttgtagga ttttatcctt aaactgtgcc aaaaactcaa aaccgtctaa
    12241 gccaaattgg gatgggaaga agctatagcc gtattggagg aggcttttct cgtctgtcac
    12301 tgaaataatg ataacgaata caaaaggtag gatacaagag agggcaatca aacccgaaat
    12361 gatactgaag aagatatctg ctttcttact gaaggagtga atgccgacat tatcaatttt
    12421 ttctttttta attttctttt ttgccatatt ctcctccttt ctagaacaaa gctgagtttg
    12481 gatcgactcg tcttgcaagc aagtttgata ggataaccag aatcaaacca acaacggatt
    12541 ggtaaagacc ggctgctgca gccataccaa tatctgctgt ctgagtcaaa ccattaaaga
    12601 catatacgtc caaaacgttg gttacattgt aaagctgacc agcattgtgt gggatttgat
    12661 agaagagacc gaagtctgcg cggaagatat ttccgactgc aaggatggtc aatacagtta
    12721 caagcggagt caactgagga atggttacgt tgcgaatacg ttgccacttg ctagctccgt
    12781 ccactgtcgc tgcttcgtag taggttggat caattcccat gatcgtcgca tagtacatga
    12841 cactgctata tccaaagcct ttccaaatac ctaggaaaag taggagatag ggccagatgc
    12901 ccaggtcagc gtagaaattg acttctttga gaccaagact ttccaataga tgattgaaca
    12961 cccctttatc aatatttagg aaggcatctg taaagaaact gatgataacc caagacaaga
    13021 agtaagggaa caacatagaa gtttggaaaa tcttcaccat tctcttagaa cggagctcgc
    13081 tgaggataat ggcaatccct acagatacaa ctaaacctag aaagataaag ccaagattgt
    13141 agaggacagt atttcgtgtg ataataaagg cgtctcttga actaaataag aatctgaaat
    13201 tatcgagtcc gacccattta ctatttatga tactatctat gaaaccatta ctggtcatgt
    13261 ggtagtcttt gaaggcaacc acgttcccaa atactggaat gtaaaagaat agaatcaacc
    13321 agagtgcccc tggcaaaacc atcaagagaa agatccagtt gtctctcaat gtttttgaaa
    13381 actttttcat aatttcctcc ctttttattt tgatatccat ctaaaaattc ttttttagac
    13441 ttttgataac gattacatta ttagtatact cctatttgca ggttaggtta aactcctaat
    13501 tatagaaaaa actccacaaa ttatgtagca gatttaaaac tttatcacca ctatcaaaca
    13561 aatgtcctaa atcaattgtt tattttatct ctattagccc agtgatggcg tcactctgtt
    13621 ataagcatcc aacaacgggg tatactgaaa aatctccaga ctagggaact cagcgatagt
    13681 tcctaatctg gagattttta atatgttatt aggcgttggc tttcaactta gcaataacct
    13741 ctttaagatt atcaatcacc tctgcttcag tatgctcaga gcctttttca tctgccaaga
    13801 acaaaactgc tttttgaagt tctttttgag agttttcaag gacatcctta tctactgttt
    13861 caaggtttga gtctttaaga agtttactta attccttggc taatttcttg agtttgattt
    13921 gcagactcat cttctcctgc tgtttctttg cccgctgttt gtcctccatc cttagttgct
    13981 gactggcttt ccttaatgga ctctagggaa gcaatggcat ctttgactgt ttgcaagata
    14041 tcacgtaaac cttgctctgt caaactatca tctgcaagag ctttcttagc ctctgccaaa
    14101 accagacgtg ctgaatctgt ggtaggattc gatacacctg tcaatgatct caaaagattt
    14161 tctaaggttt gagtctgctt actaatacta gactaaaatc aaaaagcatt acataacagt
    14221 gatatgaaat caactaaaga agaaatccaa atcatcaaaa cacttttaaa agactctcgt
    14281 acagctaaat atcataaacg ccttcaaatc gttctatttt gtctgatggg caaatcttat
    14341 aaagagatta tagaactttt atagtagttt gaaataagat gtgaacatct ctatcaggaa
    14401 agtcaaatta atttatagaa atattttagc agccaaggtg tactgttata gattcaatac
    14461 act
//
LOCUS       DAVBGY010000049        13616 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVBGY010000049 DAVBGY010000000
VERSION     DAVBGY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13616
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.049"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(<1..207)
                     /locus_tag="VQ620_001562"
     CDS             complement(<1..207)
                     /locus_tag="VQ620_001562"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764157.1"
                     /translation="MENMTNPLLLVQIRVHGYLLDFASSEGQRQKHYRLKNLPQTVEL
                     TVDDVEEDVDLTLPENRSYQEADFF"
     gene            complement(275..916)
                     /locus_tag="VQ620_001563"
     CDS             complement(275..916)
                     /locus_tag="VQ620_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2764158.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(918..2948)
                     /locus_tag="VQ620_001564"
     CDS             complement(918..2948)
                     /locus_tag="VQ620_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2764159.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEVLSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQFICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFPDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSTYYQRLFTDYL
                     QNFSSESVEVTSQKYYLPQVSLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(3217..4116)
                     /locus_tag="VQ620_001565"
     CDS             complement(3217..4116)
                     /locus_tag="VQ620_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2764160.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(4188..4748)
                     /gene="rnmV"
                     /locus_tag="VQ620_001566"
     CDS             complement(4188..4748)
                     /gene="rnmV"
                     /locus_tag="VQ620_001566"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HET2764161.1"
                     /translation="MKERISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(4748..5521)
                     /locus_tag="VQ620_001567"
     CDS             complement(4748..5521)
                     /locus_tag="VQ620_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET2764162.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIRLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(5826..6491)
                     /locus_tag="VQ620_001568"
     CDS             complement(5826..6491)
                     /locus_tag="VQ620_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HET2764163.1"
                     /translation="MTILGKDTVQQSAKGESVTQEATPEYKLEITPGGDKGGNTGSSD
                     ANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAENAIEKAAKNKQDEIKGAPL
                     SDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGVQAIAMVTVPKRPVAPNAA
                     PKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALASLGLVVATSGFALLGRKTR
                     RRK"
     gene            complement(6693..6830)
                     /locus_tag="VQ620_001569"
     CDS             complement(6693..6830)
                     /locus_tag="VQ620_001569"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764164.1"
                     /translation="MICPVIILFYYKVEVVGDGTATIVFKDGSAITIPGNQLVAQDPK
                     A"
     gene            complement(7003..7137)
                     /gene="rpmH"
                     /locus_tag="VQ620_001570"
     CDS             complement(7003..7137)
                     /gene="rpmH"
                     /locus_tag="VQ620_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET2764165.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(7282..8496)
                     /locus_tag="VQ620_001571"
     CDS             complement(7282..8496)
                     /locus_tag="VQ620_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2764166.1"
                     /translation="MKEYNKSRKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(8652..8945)
                     /locus_tag="VQ620_001572"
     CDS             complement(8652..8945)
                     /locus_tag="VQ620_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764167.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMIWQLESLVPLLYPDFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            9107..9559
                     /locus_tag="VQ620_001573"
     CDS             9107..9559
                     /locus_tag="VQ620_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET2764168.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(9594..10982)
                     /locus_tag="VQ620_001574"
     CDS             complement(9594..10982)
                     /locus_tag="VQ620_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2764169.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     SASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            11048..12010
                     /locus_tag="VQ620_001575"
     CDS             11048..12010
                     /locus_tag="VQ620_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET2764170.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKNYMEG"
     gene            complement(12007..12288)
                     /locus_tag="VQ620_001576"
     CDS             complement(12007..12288)
                     /locus_tag="VQ620_001576"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764171.1"
                     /translation="MNTIERTRRLVKDCATHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     SKHVFEVVDETDEVSSKHVFEVVDRTDEVSNHTYGRATLTWFAEIFEEY"
     gene            complement(12352..13362)
                     /gene="ccpA"
                     /locus_tag="VQ620_001577"
     CDS             complement(12352..13362)
                     /gene="ccpA"
                     /locus_tag="VQ620_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HET2764172.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
BASE COUNT         3907 a         3189 c         2448 g         4072 t
ORIGIN      
        1 aaaaaaatca gcttcttgat aacttcgatt ttcgggtagg gtcaaatcca catcctcctc
       61 cacatcatcc actgtcagtt caaccgtttg aggtaaattt ttcaaacgat aatgcttttg
      121 cctttgccct tcactactag caaaatcaag caaatagcca tgcactcgga tttgaaccaa
      181 aaggagagga ttggtcatat tttccatgat gaggatatct tataaaccac ctctggcact
      241 aggataatca gataccctgc gaatacggat tttttcacac atgagcaagt ttcctcatat
      301 caatgatttc atcagcctta ttccagacta ggggattatg ggtcgcaatg atgataattc
      361 gattttcatc tttcaaatcc accaagagat tcataacctc ctctgaattt tcaggatcaa
      421 gagctgctgt tggttcatct gccaaaatca agggtggatt tttcaaaata gttttcgcaa
      481 gggcaactcg ttgggcctct cccccagata aagtataaat tttttgttct aaatccaaat
      541 accctagatt aactttttct aaagccccca cttgcctttc caaacgttct acttttgaga
      601 ttttctgacc aacaaaaccc aaatccaaat tttctttgat tgattggttt tctaagaggc
      661 caaaattttg aaagagatag cccatctgat ctcgaaaata ctcacgagtg ggaatggttt
      721 ttaaatcttt cccctgatag agaacccttc ctccatctat cttttctagc tttcccaaga
      781 tattcagcag cgtcgtcttt ccgcttccac tctttccgat taaggcataa accttgccct
      841 tctccagctt gagattcaaa ccagagaaaa tcgcgcggtc attaaatttc ttttccaatc
      901 cttgaatatc aatcatctta ttttcctttc aatactgcca tgctaccagc ttcttctttt
      961 ttgtcctgcc ttactaaaat caagagggcg ttaagcgtaa acaaagctac aactagagcg
     1021 ctaatcaaac catctcttgt caaaatacta gataggacta ggccaagcaa gagaactcct
     1081 ccttgcgcca gtaaatactt gccatgaagc tcatagattg tcataccagc aagacgttta
     1141 atcataagct cccgtctgaa ctgctcaaaa tagagaagat tcatggaatc aaataacaag
     1201 atagccgtag acagggtcaa aatcgtccca ataaggagag agtaaaactc tacccgtttg
     1261 tcatttagta ccttggcaaa aaatagctgg ctttttacca agtaagaaac cttgtgatac
     1321 agaccttgct cttttagagc tgtgatgctg tctccatatc gatctagttt caaggcacta
     1381 ttagccgtag ttccccacaa catccctgca acaggtcttg ttccagtcgc ttgcggcgtt
     1441 agaactacaa taatcggatc ttttaggtac tggcgtgttg tcttgtaccc atcattatag
     1501 aggaaacgtt cctgtcctgt ttctgtaaaa gctagactta cctgtgggag gtagtatttc
     1561 tgactcgtca cttctacact ttcagatgaa aagttttgca ggtaatctgt aaacaatctt
     1621 tggtagtagg tagactgctc tcgcaagctc tcaggcaaga ttagcccaaa ctctccctca
     1681 gacaagtttt gcatcttgtc cataaactct gaagaaacgg taatcttttc ttctatcaga
     1741 tagcgtggtg agacatagat aacattccct gacggtgtgt agtcactgag acgattgcca
     1801 tctaggtcca cttctgctcc gtctgggaaa ttgtcaacat tgctcataat ataaaaagag
     1861 tctgtattgg ctaaccgttc ttcagtaaat gtctgccact cacgattatc cttacgcgtt
     1921 ccttcttcat cggcaaatgc actagaccaa ccaaaggata gacggtaacg gtctgaggac
     1981 tggctccatt tattgctagc tctctccatt tcctgcattt cacggtagtg gggtaggaga
     2041 gctgtcgcac tcgatccgac caccaataca gctaagagtt gccccaccat catcaatgtc
     2101 atcatacgtt tgagagggag tttccctttc aatagatcca ccagactatt ttcctgtaaa
     2161 ccaagtagat agacgacact tagtaaggta gaaatccctg ccagggtcaa tccataaagt
     2221 agaagagcaa tgatgaccag ttgcaccgtt gccataaaca aggcaccttg ataccagaga
     2281 atccccaatc ccaaaagact ggataccagc actgagcaga taaactgtct cacatcttct
     2341 aacactggtc tgagagcaac tccaaacaag ctctcaccag ctattaagcg aatccctgcc
     2401 tgacgaaggg atttgatccg ataaatcagg gtcagactca taaaggtcag cagaaaaata
     2461 gctaaactca gtagcaccat aggggtggcc gccaatagta agactatcga aaatggatct
     2521 tctccattcg aaacaaagcc ttgatagcca agttctttca aggtggtctg taagctcact
     2581 ccatccaaac ttcctgacac aatcaagtaa gaccctaata aatcactcgt ttctgcactc
     2641 tccttggagg aaatggtcag accttctgga agctctccct caccataagt ggcataggta
     2701 aagagtgttt ctccagcttc atttggctca acgattcgac gggcgatgag actattatgc
     2761 tctaccgcca agtgactcaa aacctctgtc acctcttcgc gactagctac tggttgattt
     2821 atcatcattc ctgtcacatc aacagaagga tagttaccaa ataaaatctc ttctcgatgg
     2881 tcaacggaag ttatcaccat ataaagcgat accaatacca ttgaaatcaa aataaataaa
     2941 cgtttcatca tttcctccaa aaagttactt ggtcacttag tgagaaggtg accacagtca
     3001 ctgcgatgtt aagaagctta ttaagttctt tctactagca acatcttcaa atacattata
     3061 tccgtttttt caagaactat caaggacatc aatgtcctta aaatctaact atttcatatt
     3121 atttttagct tatatctaaa aaaacagttc taaagacgaa ctttagaact gttttgatac
     3181 gaggacaagg acatttttgt cactgattca ctatgtttat aaatattttt tgacatcctc
     3241 ttgccattcc tctgtcaatt tctttactag aaatccatct ccaattagct tcgcaaatgt
     3301 tttagaagac tgataaaatg attcagctaa actaaaatct ctttcttctc ttaatgcttg
     3361 cttccacaaa aacataaata caagaggttt cttttgataa tcttgagttt tatccataat
     3421 ttcttgtaaa cacgatagat aatcattaaa acaatcagtt actcctacct tttcaagaca
     3481 acacattcct gaaataagag tatctctaat tataaagaag ttttctaatt tagttacctt
     3541 tttctgttcc aaaagaaaga gcattagctt ttcgatttga tttataaatt tggcaagatc
     3601 ttttctaatg agcatttgat aaaaaaacaa acgaatcaac aacatatcat ttgttgaata
     3661 aacttctttt tctatgatct gagacatatg ttcctcaagt attaactctg catattcagg
     3721 ccttctagtt ctagaagtat ccaaacacgc ctgcaatact tcacatgcta attgttcttc
     3781 tttaggaaga ttatcaaagt atgttttata aatctcttcc aaacacgctt ccttcttatc
     3841 gtactcctct tctttaccat agattggttc acgtaaaatc tggtatttca attctaaata
     3901 agcagaagga agagctgaaa aatccggcat aaggctataa actggaactc ctagccggcg
     3961 agcaatatag gctaacttgg gcaaactcgg ttgcgaagct ccactttcaa ttcgcgacag
     4021 ttgacgaact gtcagttctt gctcatctcc acaaaaatct ggacgactaa ttcctttttc
     4081 tatccgaatc tctttaattt tttgaccaat taacatcaga cgcctcctct tctacctgct
     4141 tcgaaaaata ctgtgaaact taaaaaattg taacggctac atctttccta ctcataagat
     4201 ttcatagctt cttccacttc tgccaaagta accccaaaca actctaggcg tttgaggagt
     4261 tgcttgccgt tagaatagcc gattcggaga gtctctccga gatattctct acgcttacgg
     4321 ctgtctgccc ctgctagaaa accaaggcga atcaaatcgc tacgactaat gtcaaactga
     4381 ctctcatgtt caaattgttc tgtcacctga gctagagccg ttttcaggtc ttcatagctg
     4441 gcatgctcaa ttcccagaga acgccccttg gtcttggact tgggaacagc ttcatctcgc
     4501 ttgagaaagg catgctgaac tgttggaatg gccgtcataa tcatgcgccg aatccgctcc
     4561 ccattaaaat ctgggtctgt aaagacaatg actccatgac gttggtgtag acgctgaatc
     4621 cgctctatgt cctggtcatt gatggcagaa cctcgagtct cataggtctc cacatcgaaa
     4681 taacgcttga gattgaccgt atcatcacga ccttcaacca cgataacttg ggaaattctc
     4741 tctttcatta cttgctgtcc aatccaaaaa tgcgttctgc atttgcagtc gttgctaccg
     4801 ccagctcttc tgtcgtcata ccacgcaagt cagcgataaa gtcgaccaca tagcgagtat
     4861 aggctgtttt attttcacga ccacgcttgg gtacaggtgc taagtaaggc gcatctgttt
     4921 ctaccaacat cttgtccaaa ggtaactctt tagctgcttc ttggaggtca gttgccttct
     4981 tgaaggtcac cactcctgag aaggaaatgg tcataccaag atccacaaac ttctctgccc
     5041 actcaagcgt ccctgaaaat gaatgcatga taccaccacg aggaccaacg ccctcactct
     5101 tgataatctc ataggtatct tccagcgcat cacgggtatg gacaacaaaa ggcaaatcca
     5161 agtccttaga tagccgaatc tgacggcgaa aaacctgctc ctgcacctct ttgggcgctg
     5221 tcatccaatg gtaatctaag ccaatttcac ctaaagccac aaccttggga tgttttaact
     5281 tatccaacaa gtaagcctca acttcctctg tataagtacc agcttctgta ggatgccaac
     5341 caatagtcgc atagagctgc tcatactcat ctaccaactc caaggcacgc tcaatcgtcg
     5401 gtttatcaaa accaacaata ttcatctgta tcacacccat ctcagcagcc aaggcaattt
     5461 cttctgcctc acgacctgca aattcttcta cattcaagtg tgtatgtgta tcaaaaatca
     5521 tctcttctaa cctcgttttc tatcttctat tataccaaaa aagaggaggg gcacctaatt
     5581 tttcggtttc ccctcctctc ttcaatagag agctattctg ctatcttttc tatccgatat
     5641 tgcccatctc ctattccaca gttagagaca gaagagattg gctacattga tagaatattc
     5701 ctattcagct aaagtatgtg attatcttgg gttctcactt ttctcaccca tcactaggat
     5761 tgatgacttc tcaaaatccc ccttataatc taactttaag tagagaatag aattttctag
     5821 ctgttctatt ttctacgtct agtctttctt cctagcaaag caaaaccact tgttgccacc
     5881 actagaccaa gactagcaag tgctgcactt gatgctgaac ctgtgttagg taattgtttg
     5941 ccagcaggtg actggtaggt aacatcttgc attgttcctg cagttgcttg cggtgcactt
     6001 gttgtcttag gagcagcatt aggagccact ggtctcttag gaactgtaac catggcaatg
     6061 gcttgcactc caatcgtttc tgcttccttc acatcttcca tagttttcgc attttcaatc
     6121 tctttgagag ctgcttgttt ttctgcttcc actcttgcta aaagttctgc tttttcttta
     6181 tcagaaagcg gtgcgccttt gatttcatcc tgcttgttct tggctgcttt ttcaatggca
     6241 ttttcagctg attctttttc agtagctaat tgcttagaag cttgtgattg agctgagttt
     6301 tgtgaacctg tgtgagctga tccacccgcc tggctaccac cgccttcatt cgcattagca
     6361 tctgagcttc cagtattgcc tcccttatct ccacctggtg taatttctag cttatactct
     6421 ggtgtagctt cttgagttac agattcacct ttcgcagatt gttgaactgt atcttttcct
     6481 agaatcgtca ctactgaacc atctgggaat gtgattgttg ctgtaccatc tccagctaca
     6541 ttgattgtcg ctccgtctaa tgctgaacca tttgcttgta aaatagcaac cttaactttt
     6601 tcttcatctg ttaaatgagt tatatctttt acatctactc tttgagcagg tgctttaaca
     6661 gttgattttt cagcagtcgg tttagtgcta tcttatgctt ttggatcttg tgctaccaat
     6721 tgatttcctg gaattgtaat agctgaacca tctttaaaga cgattgtcgc tgtgccatct
     6781 ccaactactt caactttata gtaaaataaa ataataacag gacaaatcaa tcaggacaat
     6841 caaatcgatt tctaacaatg ttttagaaac agaggtgtac tattctattt tcaatctact
     6901 gtaacatcgc ttccgaatcc atttgcatct actgaacttt tttacaaaaa taaaaatcag
     6961 tagactcgag ttcctactga tttttgatag aattcgtttg gattatgcag ccaaaacttt
     7021 gcgtccttta cgacgacgag ctgccaatac gcgacgacca tttttagttg acatacggtt
     7081 acggaatccg tgtttgcgcg cacgacgaag tttacttggt tgataagtac gtttcacgat
     7141 gaatacctcc tcatagattt tgtattcgtt tagccggcta taaagtgatc agttactaaa
     7201 catactttac tattctatct gattctttca cttttgtcaa tagctctagg caaatgtttc
     7261 cagctttttc gaatgcaagc cctatctacg atactgtttc aagaaacgag tcatcttttc
     7321 ttggatttgg gctaactcat caacacgagg aaggtaaacg atacggaagt ggtctggttc
     7381 ctgccagtta aagcctcgac catgaaccaa gagaaccttt tcctgcttca agaaatcaag
     7441 gacaaactgc tcatcatcat cgatacggta catattgcgg tcgatttttg ggaagatata
     7501 gagccccgcc ttgggtttaa cggcagacaa acctggaata tcttgaatgg cattatagat
     7561 gaaatttctt tgctcgtaga ttcgtccacc aggaagaagc aattcatcga ctgattggtg
     7621 accccccaag gaagtttgta cgacttgttg ggccaaaacg ttagagcaaa ggcgcatatt
     7681 ggacagcata ttgagccctt cgatatagcc cttaacatga gtcttagggc cagacaagac
     7741 catccaaccc acacggaaac ctgctatgcg gtgggatttt gacagaccat tcatgctgac
     7801 acagaagaca tctggtgcca agctcgccac aggcgtatgc acatgtccgt ccattaccat
     7861 gcggtcatag atttcatccg caaagataat caaatcgttt tgacgggcaa tctcaataat
     7921 ctccagcaag agttccttag gataaagggc tccagttggg ttatttggat tgataaggac
     7981 aattgccttg gtattggaag tgatttttga cttgatatcg tcaatatctg ggtaccattc
     8041 cacagcttca tcacagatat agtgaacggc atttcctcca gctaggctga cagccgccgt
     8101 ccatagagga tagtctggca ttggcactaa gacctcatcc ccattgtcca aaagcccctg
     8161 catggacatg acaatcaact cactgacacc atttccaagg taaatatcat caatatctac
     8221 gtttgggaat ttcttcagct ggcaatactg catgatggcc ttacgggctg agaaaatccc
     8281 tttggagtca gaatagcctt cactatcacg cgcattcata atcaagtcat gaatgacctc
     8341 gtctggcgct gtaaagccaa attctgctgg atttcctgta ttcagacgta aaatcttttc
     8401 tccattcgct cgcatccgca tggcttcttc caatacagga ccacggatat cataagcaac
     8461 atgctctaac ttacgagact tgttatattc tttcatcttg tttcctcaat tcatcaggat
     8521 agtaacatta taccactaga aagagaatgc gacaagttta ctgaaatgtg ttactagttt
     8581 cgtatagaag aaaaaccttg ctaaaggcga ggttaagaac gtagagatag caggtatttt
     8641 gcaaagacac atcacatacc aaaagataaa cctagaaaaa gcaagcctag aaaggagtca
     8701 taaagaagat taaacatgag gaaaagcccc catatcatca agtaatccca acgcccactt
     8761 cgttttgcaa ctagaaataa gattagatgg tagagtacat gaaagactaa aaatccaata
     8821 aaatctggat aaagaagcgg taccagactc tctaattgcc agatcatgat cacttctacc
     8881 aagactgaaa ctaggataat tccataataa aggaaatttg atttttgaaa agagtttttc
     8941 atcattctcc cttccttcac ttctttcctg ctatcctttc ttttatttta tcatatattt
     9001 atatagattt taagccctaa tattcttttg aaaaatactt tttttgcttt acttctatag
     9061 tgaaaaggtt tacaattata gtaagaaaat tgcaggaggt ttcattatgg cacaacgtta
     9121 ccaaaatatc atggtcgcaa tcgatggttc taaggaagcg gacttggctt ttgtcaaggg
     9181 agttcattct gctctacgaa acgacgctaa actcaccatc gcacatgtca ttgacacacg
     9241 cgctctccaa agcgtatcca cctttgatgc tgaagtttac gaagaactcc aagtcgacgc
     9301 tgaaagtctg atgaaagagt acgaaaaacg tgctaaagat gctggggtag cagatgttca
     9361 tatcgtcatt gaaatgggaa atccaaagac cctgctagca cgtactattc cagatgccga
     9421 ggaagtggac ctcatcctcg ttggcgcaac tggtctcaac gcctttgaac gcctcttggt
     9481 cggctcttca tctgaataca tactccgcca tgctaaggtc gatttgctgg ttgtgagaga
     9541 acaagaaaaa accttataat cacaaagaaa aggagcccct agctcctttt tgtttacgat
     9601 ttatttctct ctttatggcg ttcgtaagcc ttgagctggc gctgcagttc ctttttaata
     9661 gcaggttctg gagcatattt ttcttcccaa ttatctggtt ttaagatttt atgggtcact
     9721 ggatcaaaat gagccttacc atctggaaaa attttcccca tattggcctg atggacaata
     9781 tcaaaaatac gttctgggtc cacccccatc aagacaaaac tgccgtaggt gaagtaaagc
     9841 gtgtcaatca aggcatccac ttgccctatc aaatcttgct gagcaggtgt cttcttggct
     9901 actttacctg ctgccttatc aagggcctga tgaagttgcg atacagcttg accaaaatct
     9961 tcttcagaag gactggctga tcgaacaaac tccaccaatt cttctatttt aaaaccagcc
    10021 ctatgggttg caccctctaa atcccaagct cgaggttctt cttgggttcg ttcatccatc
    10081 atgtggtgga aggtcttgac cttattgaaa tgatagtcac ggctgacaaa gactttttct
    10141 gaagacaaaa caccaaaatg ttccaaggcc ttgtggatcc catcttgttg attgctagtg
    10201 gtaatgtgct tggcagcttc cttgacactg ctactaccat ttcccatagc gaccgacata
    10261 ccgacgccag ccagcatttc aaggtcatta tctgagtcac caaaagccat gacttggttg
    10321 aggtcaaagc catattcttt cccaactcgg cgaatgcctt ctaatttaga atttccctga
    10381 ttgatgacat ccgatgcaaa aggattgcta cgtgtcaatt tcaagtcttc aaaatcagct
    10441 gccgccttct cagattcttc tggtgtcatc agcatcaaaa cttggtaaat aggttgattc
    10501 atcaggtgaa gcaggtcctc ttccttttgg ggaacaacct tgctgaccat gcgattaaaa
    10561 gactgactca ccgtccgagt taaaacagag ggaacgaagc gactaattcg ttgggaaaaa
    10621 gaacccagac caaaggacat gattttagaa cccaacatgg catccttggt ccctaaggca
    10681 atctccgtgc cctctttttt agcatagcta attagatggc gcaaatgtaa cttggaaata
    10741 gggctcgtga acaagactct gtctttacta aagatatact ggccattata ggttaccgca
    10801 aaatccagat ccaaatcgtc catcaattcc ttaacaaaaa aaggtcctcg ccctgtcgct
    10861 acgccaacta gtaccccttg ttctttgaca atcttaatcg catccttagt ggatttcaaa
    10921 acactcttgc gattgttgac caaggttcca tcgatatcaa aaaaaacagc tttgacttcc
    10981 atcctatccc aatctcccct tttgtgatac aatgattata ccacatttca gaaagagtga
    11041 gtaaatcatg cctaagaaaa tccttgtttt acatacgggt ggaactattt ccatgcaggc
    11101 cgatgcttct ggcgctgttg tgacgagttc agataatccc atgaaccatg tgtccaaccc
    11161 acttgaagga atccaagtcc acgccttgga cttttttaac cttccaagtc cccatatcaa
    11221 acccaaacat atgctggtcc tctaccagaa aattaaagag gaagcagata actacgatgg
    11281 agtggtaatc acacacggaa ccgatacttt agaggaaaca gcctatttcc ttgataccat
    11341 ggaagttccc catatgccta tcgttctaac aggagccatg cgtagctcca atgagctcgg
    11401 tagtgatggt gtttataatt acctaagtgc tttacgagtg gccagcgatg acagggctgc
    11461 tgacaaagga gttttggtcg ttatgaacga tgaaatccac gctgccaagt atgtcaccaa
    11521 aacacatacg actaatgtca gcaccttcca gactccaaca catggccccc tcggtctcat
    11581 catgaaacag gaaatcctct acttcaaaac agctgaacct cgtgttcgct ttgaccttga
    11641 tcacatacaa ggtttagtcc ctatcatctc ggcttatgct ggtatgacag atgagctgat
    11701 tgatatgctg gatttagaac acttggacgg tttgattatc caagccttcg gagctggtaa
    11761 tattcccaaa gaaacggctc aaaaattaga aagccttctg caaaaaggaa tcccagtcgc
    11821 tctggtatca cgatgcttta acggtattgc cgagcctgtt tatgcctacc agggtggggg
    11881 cgtacagttg caaaaagcag gcgttttctt tgttaaagaa ctcaacgccc aaaaagctcg
    11941 cttgaaactc ctcatcgccc tcaatgccgg actaacagga caggctttga aaaactatat
    12001 ggaaggctaa tactcttcga aaatctctgc aaaccacgtc agagtcgccc taccgtatgt
    12061 atggttactg acttcgtcag ttctatctac aacctcaaaa acatgttttg agctgacttc
    12121 gtcagtttca tctacaacct caaaaacatg ttttgagctg acttcgtcag ttctatctgc
    12181 aacctcaaag cagtgctttg agctgacttc gtcagttcta tctacaacct caaaacagtg
    12241 tgttgcgcag tctttaacca gccgcctagt ccgctctatg gtattcatta agtcaacatc
    12301 tcttgtttaa gagcacaaaa tcaggaaatc ttctcgattc cctgattttt tctatttacg
    12361 ttttcgtgtt gagctacgtt ctgtcaaacc atgaggtaag agaacttcac gttcttccaa
    12421 ctcttcctta tgcataatct tggtcaacat acgcatacta atggcaccaa ggtcataaag
    12481 aggttgggca atcgttgtca agtttggacg ggtaaagcgt gagatttgtg aatcatcact
    12541 agtaataatt tcaaaatctt ctggcacaga aacaccctta tcagccaaac cgttcaagac
    12601 tcctgctgcc aactcatcac ctgtcacaac tgctgcagtt gcatttgatg aaatcaaacg
    12661 ctctgctaag gcgtaaccat catcatagct atatttagat tcaaatacca aaccctcact
    12721 ataagtgatt cctgcttttt tcaaggtttc cttgtagcca actaaacgaa ccttaccatt
    12781 gatgtcatcc actagcggac cgctaacgaa agcaatacgc tcattttctt tagcaaggta
    12841 actcactgca tcaattgttg cttgcttata gtcaatattg acacttggca actggtgctc
    12901 aacatcgaca gttcctgcga gaacaatcgg agtacgtgaa cgcgaaaatt ctgagcgaat
    12961 tttatctgtc aagtgataac ccatatagat aatgccatct acctgctttg aaaagagggt
    13021 attgacaaca gaaacttctt tctcgttatc ttcatcgcta ttagctagga caatattgta
    13081 cttgtacatt tctgcaatat catcaatccc cttagccaaa ctcgaaaaat aaccattggt
    13141 aatatttgga atcacgacac ctacagtggt tgtcttttta cttgcaagac cacgcgcaac
    13201 tgcatttgga cgataatcta agcgatcaat tacctctagc acttttttac gggtattctc
    13261 ttttacattt ttattgccat tgaccacacg gctgaccgtc gccatggaaa cacctgcttc
    13321 acgagcgaca tcataaatgg ttactgtatc atctgcattc attccttttc ctgtcctttc
    13381 tatctcacac attcttttac aagtagaggt actgattgaa gctctatatc tacttacaaa
    13441 agtgaagatg tgaaaatttc gttttcatat ttctacttat tccattctat cactaattgt
    13501 aaacactttc aagtgttttt tgaagattga ttgaaaaaat ttcatagaaa acctaggttt
    13561 agctccttgc taccacctta gactaaataa aaaggaggaa actaagccct tctaaa
//
LOCUS       DAVBGY010000050        13294 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVBGY010000050 DAVBGY010000000
VERSION     DAVBGY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13294
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.050"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            61..717
                     /locus_tag="VQ620_001578"
     CDS             61..717
                     /locus_tag="VQ620_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET2764173.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILVVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            707..1030
                     /locus_tag="VQ620_001579"
     CDS             707..1030
                     /locus_tag="VQ620_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET2764174.1"
                     /translation="MVSKYLLLAVIFSGLVTWIPRMIPFILVKHKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAIFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            1134..1964
                     /locus_tag="VQ620_001580"
     CDS             1134..1964
                     /locus_tag="VQ620_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2764175.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPKPFIYEENGELTGYEIEVVRAIFKDSDKYNVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2118..2972
                     /locus_tag="VQ620_001581"
     CDS             2118..2972
                     /locus_tag="VQ620_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HET2764176.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            3072..4445
                     /locus_tag="VQ620_001582"
     CDS             3072..4445
                     /locus_tag="VQ620_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET2764177.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4438..5499
                     /locus_tag="VQ620_001583"
     CDS             4438..5499
                     /locus_tag="VQ620_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HET2764178.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGSSTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            5501..6193
                     /locus_tag="VQ620_001584"
     CDS             5501..6193
                     /locus_tag="VQ620_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2764179.1"
                     /translation="MESLIQTYLPNVYKMGWSGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAIPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6222..6791)
                     /locus_tag="VQ620_001585"
     CDS             complement(6222..6791)
                     /locus_tag="VQ620_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET2764180.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            6926..7540
                     /locus_tag="VQ620_001586"
     CDS             6926..7540
                     /locus_tag="VQ620_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET2764181.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKILLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7542..9188
                     /locus_tag="VQ620_001587"
     CDS             7542..9188
                     /locus_tag="VQ620_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2764182.1"
                     /translation="MKHFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVDSIQAIAQGQEGLRIDTLEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9200..10486
                     /locus_tag="VQ620_001588"
     CDS             9200..10486
                     /locus_tag="VQ620_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2764183.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10538..11071
                     /locus_tag="VQ620_001589"
     CDS             10538..11071
                     /locus_tag="VQ620_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2764184.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            11149..11619
                     /gene="nrdI"
                     /locus_tag="VQ620_001590"
     CDS             11149..11619
                     /gene="nrdI"
                     /locus_tag="VQ620_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HET2764185.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(11912..13174)
                     /locus_tag="VQ620_001591"
     CDS             complement(11912..13174)
                     /locus_tag="VQ620_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET2764186.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         3894 a         2494 c         3057 g         3849 t
ORIGIN      
        1 taagacaata tgaggagaaa gatgaaagaa aaaggatttt aggagggggc gcaggcagcc
       61 atgccaacgg ctcttggcta tgtcagtatc ggcctggcct gtgggactat cggtgcgccc
      121 tatgtgacac ctgttgagat gggcttgatg agtctctttg tttatgctgg gagtgcccag
      181 tttgccatgt tggcactgat tgtggttcaa gctcctgtgg cagctattgc tatgacggtt
      241 tttctaatca acttgcgtct ctttttgttg agtttacacg catcgactta tttccgtcat
      301 accagtcttt ggtacaatat cggtatgtct agtatcttga cagatgagac ctatggcgtt
      361 ttgatgggtg aattggccca tacagacaag gtaaatccta tgtggatgca cgggaacaat
      421 ctcaacagct atgtggcttg gtttgtgggg acagtagtcg gaacggctct gggtggcctg
      481 ctaccaaatc cagaaatctt tggcttggat tttgccctgg ttgggatgtt tattggtatt
      541 tttgcttcgc aatttcagat tatgcaaaga cggattcctg tccgcaatct gctcattatc
      601 ctagtagttg ttgcggtgtc cttctttttg ctcttgacag tggtgtctca gtcactagct
      661 gttctgtttg cgacgctact tggttgtagc atgggggtgg ttttagatgg tcagtaagta
      721 tcttttatta gcagttattt tctctggtct tgtgacctgg attccccgta tgattccctt
      781 catcttggtc aagcataagg gcctgcctgc gattgttgag cgttttttga agtttttgcc
      841 tgtttccatt atctttgcct tgattctttc aagcgtagtg acaggtaagg ttgggagcct
      901 tcctcaaatt aaatggctag acttcttagc catctttcca acagcttggg tagcctttcg
      961 ctaccgcaat ctagtcggaa cagttctctt tggagtggtc ttgattgcca tcttgcgttt
     1021 ggtcttttaa attacccata aaaaaaactt atcacagaga tagatatcat ataatggcgt
     1081 aaatgctctt tttctgttaa gattataagg tattctattt tggaggaaat gacatgaaaa
     1141 aaatcgttaa atactcatct cttgcagccc ttgctcttgt tgctgcaggt gtgcttgcgg
     1201 cttgctcagg tggtgctaag aaagaaggac aagcagctag caagaaagaa atcatcgttg
     1261 caaccaatgg atcaccaaag ccatttatct atgaagaaaa tggcgaattg actggttatg
     1321 agattgaagt cgttcgcgct atctttaaag attctgacaa atataatgtc aagtttgaaa
     1381 agacagaatg gtcaggtgtc tttgctggtc ttgacgctga ccgttacaat atggctgtca
     1441 acaatcttag ctacactaaa gaacgtgcgg agaaatacct ctatgccgca ccaattgccc
     1501 aaaatcctaa tgtccttgtc gtgaagaaag atgactctag tatcaagtct ctcgatgata
     1561 tcggtggaaa atcgacggaa gtcgttcaag ccactacatc agctaagcag ttagaagcat
     1621 acaatgctga acacacggac aacccaacta tccttaacta tactaaggca gacttccaac
     1681 aaatcatggt acgtttgagc gatggacaat ttgactacaa gatttttgat aaaatcggtg
     1741 ttgaaacagt gatcaagaac caaggtttgg acaacttgaa agttatcgaa cttccaagcg
     1801 accaacaacc gtacgtttac ccacttcttg ctcaaggtca agatgagttg aaatcatttg
     1861 tagacaaacg cattaaagaa ctctacaaag acggaactct tgaaaaattg tctaaacaat
     1921 tcttcggaga cacttatcta ccggcagaag ctgatattaa ataatttctt gaaatcatcc
     1981 attctgagta agatgggtgg tttctcagtt tttagggata tagttttaaa ctatatatct
     2041 gactatctaa taacaatttg tgaaatcaaa caaattgtga gatactagta cggtattatt
     2101 tttaaggaga aagaatcatg aaaatcaaaa aatggcttgg cctagcagcc cttgctacag
     2161 tcgcaggttt ggctcttgca gcttgcggaa actcagaaaa gaaagcagac aatgcaacaa
     2221 ctatcaaaat cgcaactgtt aaccgtagcg gttctgaaga aaaacgttgg gacaaaatcc
     2281 aagaattggt taaaaaagac ggaattacct tggaatttac agagttcaca gactactcac
     2341 aaccaaacaa agcaactgct gatggcgaag tagatttgaa cgctttccaa cactataact
     2401 tcttgaacaa ctggaacaaa gaaaacggaa aagaccttgt agcgattgca gatacttaca
     2461 tctctccaat ccgcctttac tcaggtttga atggaagtgc caacaagtac actaaagtag
     2521 aagacatccc agcaaacgga gaaatcgctg taccgaatga cgctacaaat gaaagccgtg
     2581 cgctttactt gcttcaatca gctggcttga ttaaattgga tgtttctgga actgctcttg
     2641 caacagttgc caacatcaaa gaaaatccaa agaatttgaa aatcactgaa ttggacgcta
     2701 gccaaacagc tcgttcattg tcatcagttg acgctgccgt tgtaaacaat accttcgtta
     2761 cagaagcaaa attggactac aagaaagcac ttttcaaaga acaagctgat gaaaactcaa
     2821 aacaatggta caacatcatt gttgcgaaaa aagattggga aacatcacct aaggctgatg
     2881 ctatcaagaa agtaatcgca gcttaccaca cagatgacgt gaaaaaagtt atcgaagaat
     2941 catcagatgg tttggatcaa ccagtttggt aataagaaac agggaggtgg gagagaaaat
     3001 tccacctctt gcttttgtat agagtataga ttgtaaagaa gactattcgt tcatagaaag
     3061 gtagagagaa tatggttttt cctagcgaac aagaacagat tgaaaaattt gaaaaggatc
     3121 atgtagtcca acattatttt gaggttttgc gtaccttgat ttctaagaaa tcagtctttg
     3181 cccagcaggt tggactcaag gaagtcgcaa attatctggg tgagattttc aagcgtgttg
     3241 gagctgaagt ggagattgat gagagctata cagcgccctt tgtcatggca catttcaaga
     3301 gttcgcgtcc agatgccaag accttgattt tctataacca ctatgacact gtgccagcgg
     3361 atggggatca ggtctggaca gaggatcctt ttacgctttc ggtccgcaat ggcttcatgt
     3421 atgggcgtgg ggttgatgac gacaagggtc atatcacagc tcgcttgagt gctttgagaa
     3481 aatatatgca gcaccatgat gatttacctg tcaatatcag ctttatcatg gagggagcgg
     3541 aggaatcggc ttcaacagac ctagataagt atttggaaaa gcatgcagac aaactccgtg
     3601 gggcggattt gttggtctgg gaacaaggga ccaaaaatgc cttggaacag ctggaaattt
     3661 ctggtggcaa taaggggatt gtgacctttg atgccaaggt aaaaagcgct gatgtggata
     3721 tccactcgag ttatggtggt gttgtggaat cagctccttg gtatctcctc caagccttac
     3781 agtctcttcg tgctgcggat ggccgtatct tggttgaagg cttgtacgaa gaagtacaag
     3841 agcccaatga acgagaaatg gccttgctag aaacttatgg tcaacgaaac ccagaggaag
     3901 ttagtcggat ttatggattg gagttgcctc tcttacagga ggagcggatg gcctttctaa
     3961 aacgtttctt tttcgagcca gcgcttaata tcgaaggaat ccagtctggt tatcaaggtc
     4021 agggtgttaa gactatttta cctgcagaag ccagtgccaa gctagaggtt cgtctggttc
     4081 cgggcctaga accgcatgat gttctggaaa aaattcggaa acagctagac aaaaatggct
     4141 ttgataaggt agaattatac tataccttgg gagagatgag ctatcgaagc gatatgagcg
     4201 caccagccat tctcaatgtg atcgagttgg ccaagaaatt ctatccacag ggcgtttcag
     4261 tcttgccgac gacagcgggg acaggaccta tgcatacggt ctttgatgcc ctagaggtac
     4321 caatggttgc attcggtcta ggaaatgcca atagccgaga ccacggtgga gatgaaaatg
     4381 tgcgaatcgc tgattattac acccatatcg aattagtaga ggagctgatt agaagctatg
     4441 agtagagata ttatcaagtt agatcagatc gatgtgactt ttcaccaaaa gaagagaacc
     4501 attacagcgg ttaaggatgt gaccattcac atccaagaag gggatatcta cggaatcgtt
     4561 ggatattctg gagcaggaaa atcaaccctt gtacgggtga ttaatctctt gcaaaaacca
     4621 tctgcaggga aaattaccat tgacgacgat gtgatttttg acggcaaggt gaccttgacg
     4681 gcagagcagt tgcgtcgtaa acgtcaagat atcggaatga ttttccagca ttttaacctg
     4741 atgagccaaa agacagcaga ggagaatgta gcctttgccc ttaaacactc tgaactcagc
     4801 aaggaagaaa agaaggctaa agtagctaag ttgttggact tggttggttt ggcagatcgt
     4861 gctgaaaact acccttcaca actatctgga gggcaaaaac agcgtgtggc aattgcgcgt
     4921 gccttggcca atgatccaaa aatcttgatt tcagacgagt caacttctgc ccttgatccg
     4981 aagacaacca agcagatttt ggccttgttg caagatttga accaaaaatt aggcttgact
     5041 gttgtcttga ttacgcatga aatgcagatt gtcaaagaca ttgccaaccg tgtggcggtt
     5101 atgcaggatg ggcatttgat tgaagagggt agtgtgcttg aaatcttctc agaccctaaa
     5161 caacctttga ctcaagactt tatctcaaca gctacaggta ttgacgaagc catggtcaaa
     5221 atcgagaagc aagaaatcgt ggaacacttg tctgaaaaca gtctcttggt gcaactcaag
     5281 tacgctggat cttcaacaga cgagccactt ttgaatgaat tgtacaagca ttatcaagta
     5341 atggctaata ttctctatgg gaatatcgaa atcctcgatg gtactcctgt tggagaattg
     5401 gtggtggtct tgtcaggtga aaaagcagcg ttggcaggtg cccaagaagc cattcgtcaa
     5461 gcaggcgtac agttaaaagt attgaaggga ggacagtaag atggaatcat tgattcaaac
     5521 ctatttgcca aatgtctata agatgggttg gtctggtcag gcaggctggg gaacagctat
     5581 ctacctaacc ctctatatga cagttctttc cttcattatc ggaggcttct tggggctagt
     5641 ggcaggtctc tttctcgtct tgacagcgcc aggtggtgtc ttggagaata aagtcgtatt
     5701 ctggatttta gacaaaatta cctcaatttt tcgtgcgatt ccctttatca tcctcttggc
     5761 aatcttgtca ccactttctc acttgattgt taaaacaagt atcgggccaa atgcagccct
     5821 tgtcccactt tcttttgcag tctttgcctt ctttgcccgt caggtgcagg ttgtcttggc
     5881 tgaattggat ggcggtgtca ttgaggcggc tcaagcgagc ggagcgactt tctgggacat
     5941 cgtgggtgtt tacctatcag aaggtcttcc agatttgatc cgtgtgacga ctgtgacctt
     6001 gatttccctt gttggggaaa cagctatggc cggtgcggtt ggagctggtg gtatcggtaa
     6061 cgtagccatc gcttatggat ttaaccgcta caatcacgat gtgaccatct tggcaaccat
     6121 tattatcatt ttgattatct ttgcaatcca attcttggga gatttcttga ctaagaaatt
     6181 gagtcataaa taaaaagagc cgcgtggctc tttttaactg atcagatttt ctgtgcaaat
     6241 tttttactca aggcttgtcc aatcaaggca cccactaggg ctccgatgac aatacttgcg
     6301 ataaatagaa ggacagttcc agggtttgga gcgaccatga tacggtcgat atattcttgg
     6361 gattttcctc ttgccagaag agtagccata taggctttgg gcgcaatcca cataagcaag
     6421 attggtcctg ttgtactaaa ggcgaaaata atgaaagaaa ggaagttctt tgttttgtcc
     6481 ttgtattttc ctaaatgagc tacttcatct gctaggaggc cacagataat tccaggaagg
     6541 aaggaaccag caccgtgttt agttcccaag aaaaagaggg caatgacaag gccgatagtg
     6601 gtaatggctc caaagcgcgg aacttttgcg actaggatca tatagacgct accgccgaca
     6661 agggcagtaa aggcaggcgc ataaaacatg tttccagttt ggtcaaagag attgcccaaa
     6721 aggacaccaa tccccatgca gaggaagtaa agaactgcaa aaagcagtgt agttaagata
     6781 gatttcttca tgaattgtct cctgataatt ttttcacaat tctcattgta ccacggtttg
     6841 gtgggattgt aaatggatac tagaagtttt tgaaaacgct tgaaatatga tataatggtt
     6901 tcatagttat ttttagggga atatcatggg tagattttta gactttgtct ttaatcgttt
     6961 ctttttaggg atgattgcga cagccttctt ttggctatta actttagcag gagggattat
     7021 ccttggtcta gcgccggcta gtgccacctt gatgagctta tatgcagaac atggttatag
     7081 ctttcgggaa tacagtttga aggaggcttg gtctctttac aagcaaaatt ttgtctcaag
     7141 caacctgatt ttctatagct ttttaggtgt gggtctagtt ttgacctatg gtttgtatct
     7201 cttggtgcaa ttgcctcatc agaccattgt tcatttgatt gcgacccttt tgaatgtcct
     7261 agtagttgcc ctgatctttt tggcttatac agtatcttta aaattacaag tttattttgc
     7321 cttgtcctat cgaaatagtc tcaaattatc cttgattggc atctttatga gtctagcagc
     7381 tgtggccaag attctccttg ggactgtgct acttgtagca attggttact atatgcctgc
     7441 cctgctattt tttgtaggaa ttgggatgtg gcatttcttt atcagtgata tgttggaacc
     7501 tgtctatgaa atcatccatg aaaaattggc gacaaaatag aatgaagcac ttttggctac
     7561 atacgcttct aagaacctat agttcagtga tgatcattat cattgcgagt tttgcaatct
     7621 tactctctta cgctgactgg gattcacgtg aaaaggaagc ccagagagta gcccagcgtg
     7681 taactgctag aacagtgagt gaaattgaat attaccatag agagtcaacc cagatagctc
     7741 aggctttagt tgaaaaccaa gctcgtattg agggaatcta taaatacttt agtcttagca
     7801 tgccagacta tttttactgg caattagagc ggaaagcttc gccttatata tcagtctctc
     7861 tgtatgaaaa tgttgatgac ctctatgttc gaaatgattt tgtaactgga gtggccattg
     7921 cttttcaaga ttacaaggaa gtctatgttt ctactaaaga caaacgtagt ggagaaaaaa
     7981 tcagggctga ggatttcaaa ccagcaggaa atagttttgc cattccggtg tcagatccag
     8041 tgtcagatca agatttagga gtgatttaca tctccttgga tcctgctgtt ttataccatg
     8101 ccattgataa tactagaggt catactccga tggcagtaac agtgacctca ccttttgata
     8161 cggagatttt tcatattggt gagacagttg ataaggagag tgaaaattgg ctagttggct
     8221 taacttctca tggatatcag gttcaggtgg cagttcctaa aaactttgtt ttacaaggaa
     8281 cagtgactag ctctgctttg attgtgggtt tgagccttct ctttattgtc attctttatc
     8341 tgactttgag gcagaccttt gctaattatc aaaagcaggt agtggatttg gtggattcca
     8401 tccaagctat tgcccaagga caagaaggtc ttcgcattga tacgcttgaa aaggatcagg
     8461 aattgctcct aatcgcggag acgaccaatg atatgttgga tcgattggaa aagaatatcc
     8521 atgatattta ccagttagaa ctcagtcaaa aagatgccaa tatgcgggcc ttgcaggcgc
     8581 aaatcaaccc tcacttcatg tataatacgc tggagttctt gcgtatgtac gcagtcatgc
     8641 agagtcaaga tgagttggca gatatcattt atgaattcag tagtctcttg cgtaacaata
     8701 tttccgacga aagagagacc ctcctcaaac aggaattaga attttgccgt aaatacagct
     8761 atctctgcat ggttcgctat cccaagtcca ttgcctatgg tttcaagata gatccagagt
     8821 tagagaatat gaagattccc aagtttacct tgcaaccgct ggtagaaaac tatttcgcgc
     8881 atggtgttga ccacaggcgg acagataatg tgattagcat caaggctctt aaacaggatg
     8941 ggtttgtgga aattttggtg gtcgataatg gtagaggaat gtcggctgaa aagttggcaa
     9001 atatccgaga aaaattaagt cagaaatatt ttgaacacca agccagctac agtgatcaaa
     9061 ggcagtctat cgggattgtc aatgtacacg agcgttttgt gctctatttt ggagaccgct
     9121 atgccattac tatagagtct gcagagcaag ccggtgttca gtatcgtatt acaattcaag
     9181 atgagtagaa agggagaaaa tgtataaagt attattagta gatgatgagt acatggtgac
     9241 agaaggtctg aagcgtttga ttccctttga taagtgggat atggaggtcg tcgcaacagc
     9301 cagtcatgcc gatgaagctc tagaatatgt tcaggaaaat cctgtcgatg tcatcatttc
     9361 cgatgtcaat atgccagaca aaacagggct tgatatgatt cgggagatga aagagatctt
     9421 accagatgct gcctatatcc tgctctcagg ttatcaggag tttgattatg taaaaagagc
     9481 aatgaacctt agtgtggtgg actatttggt caagcctgtt gataaggtag agctgggaaa
     9541 tctgctggag aagattgcag gtcagctcgg cgagagaggg aagaaaagtc agactcttag
     9601 tcaagaatta gacgaggctg gatttgttag ttatttaggg gataaggaga attggtggat
     9661 aggtctatcc aaggaaaaac aaggttcctt caccattccc tactatgtct tgggccaaga
     9721 ctggcagatt ttcatttctg accaacccct agatggttta gtcgttacac cttttgaagc
     9781 tccttatcaa gaacactttg aacgctggaa gctgaatgct gagaaaaccc tcttttacgg
     9841 ttctgtaaat ttgcagcagt ctgagagtct ctttgcctat tacgaaccga tttatagggt
     9901 tattattcag ggaaatctca atcaaatcgt agaagagtta aatctcttgg agaaggtagt
     9961 tcttgaaaat acgccgcgtg ttccgattac taaacagctt tttattcagt ttgtcatgga
    10021 tgttttccat ttatttgaac atctcaaagc tgatgacctg acagacatcg tcaaaaccat
    10081 tcatgctatt caatccttcg atgaattggt tccttatatc aaggaaactc tgagcagctt
    10141 tttcggtcaa taccgtatga atgaaaatgt ggtcagtgtg ctggaagtca ttggtcgtga
    10201 ttaccaaaaa gagctttccc tcaaggatat cagtaaggct ctctttatca atcctgtcta
    10261 tctagggcag ttgattaagc gtgaaaccga ttcgactttt gcagagttac taaacaaaca
    10321 acgtattaag gctgcccaac aacttttgct ttcaactagt gacagcatcg aagatatttg
    10381 ttatgctgtt ggttacagta accttggata tttctataaa gttttccgaa aattgtgcgg
    10441 aaaatcgcca aaagcctacc gaaaacaggt agaaactata ctataagatt tgtattcctt
    10501 tacaaaaggt gctataatat tatcaggagg tcctatcatg aaaaagaaac caatttatct
    10561 atgggtcttg ctaatcttgt ctgcccttat ttcagttccg tctctgtttg gaattgtgag
    10621 tcccttgcct agtaaagaag cccttcgtgc tgctcagaaa caagttgcag gggtcaatgc
    10681 tcagcaatta gaagaccagc ttaattatac ctatagagta gcagaagcat ctcattctat
    10741 ttttaatgtt gctttgattg tgctatctac tattttagtg gtcgtagcga ttgtcttcct
    10801 tgttcgtaaa aatttgcaat atgcaaacta tacttacgtt ggctatgttt tgttagctat
    10861 tattggttcg atttatggct atgttggttt acaagacgct gtgcagttgg ttcaagatga
    10921 gagcatgcgt ttgacagtga gtattggttc aaaagctgtt agcattttct atatcgtcat
    10981 caatgttctc ttcctagccc ttgtcttcta taagatgtgg cgacaacaga aagccttggc
    11041 agaagaagag gaaacagaag agcttgccta agtattgaca aaaaagaact atcaagatac
    11101 aatcttggta gttgtttttt gattatagat aaaatcatgg aggtacatat gaagacaatt
    11161 tctttagttt atattagttt gagtggcaac actgaaagtt ttgtgacgcg cttgaaagac
    11221 tatctcttgt cccagtacaa agggattgag gttcaaaaga ttcatatcaa ggatttggtc
    11281 aaggaaggca aaaatttcta tgaaatggac catccctatg tcgctttttt gccgacctat
    11341 ctcgaaggtg ggaatggcgt ggataacgga gatgttgaga ttttgacgac accggtggga
    11401 gattttattg cctatggtaa caatgctagt aagtgttttg gtgtggttgg gtcaggaaat
    11461 cgtaacttta ataaccaata ctgcctaaca gccaagcaat acagtcaacg ctttggcttc
    11521 cctgtattgg ctgactttga aatgcgaggc atgctggaag atatcaaaca tgtcgcagct
    11581 attatcgcag atttgtatga gttggaaaag gagaattaat actcaatgaa aatcaaagag
    11641 caaactagga agctagtcgc aggttgctca aagcacagct ttgaggttgc agatagagct
    11701 gacgtggttt gaagagattc tcgaatagta taaagttctt ataagaggtg gttgaagttg
    11761 ttcaactgtt ttttgagtat aaacagtctt taagaacgta aaaaagcata agttcaggtc
    11821 ttcaaaacct tgttcttatg ctttttatca tagaaatatt tgctgaattt tctcttgaaa
    11881 tgaaacctta tcttcatctc gcgcttgtcc cttaccctgc ttttccgatt gcgagtgcgt
    11941 agataaagaa gatggcgaag ccaaagagga aaatcaatcc cgcaatggca aggtgtttga
    12001 gccaagaagg aagatttttg ttttcacgcg ttaccaaggc gtaattcaaa agagcaaaga
    12061 aaggtgttgt caggaaagat ccaatcatgg caaagcggag catggttgaa acctgaccag
    12121 cgaagaactt gataatgacg ataccgatga tagcagtgat ggtcatccag atgttcaaag
    12181 atttacgatt gtcctctttt tgactgatta gcagtcggag agattcctga ttaacgcgag
    12241 aatagccatc gataacagtt ataactgttc caaagataca gaggaaggca ataaaggtaa
    12301 tcaagtaacg ggaccattcg ccaagaacag aggcatacat gcccacgaat tgagagatgt
    12361 atttggctga agcagcttca accgcctgcc ctgtaggata ctgaatcagt gctcccagtg
    12421 ccacaaagaa gacggctagg atagctgttc caatataacc agtgttaaag tcaaacagag
    12481 cgtcttctgt gttaaagttg acggtctttc tcttttcagc tgaccaaagt gaattgatgg
    12541 ctgaaatttc aataggagcc ggcatccatc ctaggaggga gacgatgaag ggcagagctg
    12601 ccatttgcca aggtgtcttc tcgacaaaat cagaactgta ttctggatgc ttgaccgccg
    12661 caatgataac tgcaagaaca gtcgcaatgg ttaaggcaga cataatccat ttgaccatgc
    12721 cgtctaaaag tttgtagcct ccaaagagta gcatagccca aatgattgca acgagaatga
    12781 gggaccactg agtaatgcta agtccaatca ttgggaaggc actggcgatg atagctgagc
    12841 acagaatggc aacaccagcc gtgttgacca tagccgaaaa gacattgaga ataaagaaaa
    12901 tccagagata gagttttcct ttttcggcat aaccttcaac caaagtcttt ccagtatcag
    12961 ctgtgtattc agcaccaaaa cggaaaaatg gatatttaaa gacattggct aagatgacca
    13021 agagaagtag agaccaaccg taagaaccgc cagcttgagt tgaggataca atgtgggaac
    13081 ctccaacagc ggcagttgcc attaggattc caggtcccat tgcttttagc ttgcttgccc
    13141 aggttgattg atttaaggaa atagcttgcg acatgataaa tactcctttt tgaattttca
    13201 gaataatctg tatatgcaat ctatattcta caaaaaattt tggaaaaatg caagaatatt
    13261 ttgaaaaaag atagaaaatt tacaaagaaa atct
//
LOCUS       DAVBGY010000051        13282 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVBGY010000051 DAVBGY010000000
VERSION     DAVBGY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13282
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.051"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(98..610)
                     /locus_tag="VQ620_001592"
     CDS             complement(98..610)
                     /locus_tag="VQ620_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HET2764187.1"
                     /translation="MSAQITINHKKVRYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNFPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            complement(635..1615)
                     /locus_tag="VQ620_001593"
                     /pseudo
     CDS             complement(635..1615)
                     /locus_tag="VQ620_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            complement(2091..2900)
                     /locus_tag="VQ620_001594"
     CDS             complement(2091..2900)
                     /locus_tag="VQ620_001594"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET2764188.1"
                     /translation="MTFLDKIKETATFLKDKGIQAPEFGLILGSGLGELAEEIENPVV
                     VDYADIPNWGRSTVVGHAGKLVYGDLAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGYGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSKSYTP
                     EYRATAHEVAKKLGIKLDEGVYIGVTGPTYETPAEIRAYKILGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            complement(2897..3457)
                     /locus_tag="VQ620_001595"
     CDS             complement(2897..3457)
                     /locus_tag="VQ620_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET2764189.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAQLRQELTELGLGKVETYINSGN
                     IFFTSTDPKAQLVEKLESFFEIHYPFIQSFSLLSHEDYEAELENLPKWWTKDLARKDV
                     LFYTESLDVDQVIEKVNSLELEDEVVHFGRLGIFWGKFSEESYYATAYHKYLLKMPFY
                     RNITIRNAKTFDKIGQMLKTKKGDTQ"
     gene            complement(3459..4670)
                     /locus_tag="VQ620_001596"
     CDS             complement(3459..4670)
                     /locus_tag="VQ620_001596"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HET2764190.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     SVGLNVPNMAKIGLGNIPRETPLKTVPAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKIIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFAPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPSFKGNGVIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            complement(4694..5377)
                     /gene="rpiA"
                     /locus_tag="VQ620_001597"
     CDS             complement(4694..5377)
                     /gene="rpiA"
                     /locus_tag="VQ620_001597"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET2764191.1"
                     /translation="MENLKKMAGITAAEFIKDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTSKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRRFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIEDPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QILTSTKAN"
     gene            complement(5675..6514)
                     /locus_tag="VQ620_001598"
     CDS             complement(5675..6514)
                     /locus_tag="VQ620_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET2764192.1"
                     /translation="MKVIDQTLLEKVIIERSCTSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEAMAFSLQDQQLLQEQLEKAEVVLLGPGL
                     RDDTFGENLVKQVFASLKKNQILIVDGGALTILARTSLLFPSNQLILTPHQKEWEKLS
                     GIAIEKQNEGTTSSALTSFPQGTILVEKGPATRIWQVGQSDYYQLKVGGPYQATGGMG
                     DTLAGMIAGFAGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISNCLPKVMK
                     RYV"
     gene            complement(6529..7350)
                     /locus_tag="VQ620_001599"
     CDS             complement(6529..7350)
                     /locus_tag="VQ620_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HET2764193.1"
                     /translation="MILKIYNGEYSLQWDGIYYLALIDYPNIQEWELEKIAKFIAYEK
                     LHKRQTSIECADSCLKKEILDYICQHPFLPPFTPTDKRVASTYDLHKRLVTSDYCSHT
                     TTIDAAISIFKTGRLLSAVKAFGRDAEELVLDSRNAASDPIDYFDYVMLGWSNTSSGY
                     RLAMERLLGRAPSEKELQDKFIPGVSFHFIYTDLIKVPGYIFDGYHAVKIKDMLNLLS
                     ELYICIIPTHNKSQFENIIPTKIQDRVYYLDYAGEDLEEWTKKVYQVVLKQSDKG"
     gene            complement(7488..8345)
                     /locus_tag="VQ620_001600"
     CDS             complement(7488..8345)
                     /locus_tag="VQ620_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HET2764194.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTEKLKEETGLVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGFRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLSKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            complement(8499..9233)
                     /locus_tag="VQ620_001601"
     CDS             complement(8499..9233)
                     /locus_tag="VQ620_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2764195.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            complement(9233..9916)
                     /locus_tag="VQ620_001602"
     CDS             complement(9233..9916)
                     /locus_tag="VQ620_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2764196.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSEIVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            10106..10336
                     /locus_tag="VQ620_001603"
     CDS             10106..10336
                     /locus_tag="VQ620_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HET2764197.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            10565..12823
                     /locus_tag="VQ620_001604"
     CDS             10565..12823
                     /locus_tag="VQ620_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2764198.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIKAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(13030..>13282)
                     /locus_tag="VQ620_001605"
     CDS             complement(13030..>13282)
                     /locus_tag="VQ620_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2764199.1"
                     /translation="KFLLPTLTTPSVIIMDNARFHRMGKLELLCEEFGHKLLPLPPYS
                     PEYNPIEKTWAHIKKHLKKVLPSCCNTFYEALLSCSCFN"
BASE COUNT         3984 a         2942 c         2410 g         3946 t
ORIGIN      
        1 aattttgttc tttcacagag agctacctga gttctattca agctcagata gtactttctt
       61 ataaactaga caaactaact gtcattctac catcagatta caagacatca tcgtcactca
      121 ccttggaatt caatgtcgta ccccaatggg taattttacg gtggggttga gctaaaattg
      181 gtctgttttc atagattgtt tgccatctat tccatagtag gcccgtcttt ttctcaatct
      241 taactcgcag atttctcata ttttctttga ttgggaagtt gaggacaaaa cctgcagtct
      301 ggttgcaacc gtttccttcc caagaatgac tacgaacaac ttggtttcca tctttatcta
      361 ctggaacttc ttcccaagtt atggagtagc gggcaatgta agctccactg tgttgaattg
      421 tcaatgtttt atctttcaca ggagtctgac tgattggttg aactggctta gaaacttgtg
      481 tcgccgtttc agcattcgta gctataaagc ggaaaacttc aacttctgca agactgaggg
      541 cattatagcc ttctagctca atccgaacat agcgcacttt tttatggtta atcgtaattt
      601 gtgctgacac atctttagga gatgttatac gtttttactc aatttcttta ccagaactgt
      661 ctaaaagaat gacatcaaag tttgccaatc tatcctgggc agtgtctgtt cggttgtaaa
      721 tattgatttg gcgaatggtt tcttctttag ccaaatctac ttgccaccaa ggcttagatt
      781 ggaagtttgt atgagtgaca gaattgtgac catagttacc atcgactttg ccatccacag
      841 ctcttctagc atctcctccg aaaactgtcg aactttgagt aacctgtttt cctaaggcaa
      901 tattttccat tggttcagcg cttggttgac tagaagatcg aggcagagcc tgtttcttaa
      961 agaatcgcac tttatccaat ttcggatcac gataaccatg tttatccaag gtagcttgtt
     1021 taccaatatt aaagttagga atatcattca ttggactttc aaaatagcca cgagaacggt
     1081 gtttaaagcg tgaattacct gtaaatgtaa caatatcacg attattatga atcaagggag
     1141 tcacaacatt gtcattatca accacataat gcttaatttt tccactaaca gctaatttac
     1201 gactttccaa accaacatcc cagaaaccat tcttagcatt ccaaacagtt ctggaagtaa
     1261 tcactttagg agcactgaaa gttgtcactt tcctcaatac atggttataa aaatcaggat
     1321 atttttggta agcttcaacc gctgcaatct gagctaggta acatccaaga gaatgacctg
     1381 tcatatacaa cttagtaata cttatatcct tggctaattc cccaacaacc ttgcggatat
     1441 catccgcttg agctggttat gttatctcta cttttgactc ttcccttttg ttttcaagaa
     1501 cttcacttgc ttccggaagc gtttgaggag ctagtcttgg ctcctctcta agaggagttt
     1561 ctagttttgg agcatcattt tctgactctt tcaattcctc cttagaatct agcacaccta
     1621 ttaccacttc agatgttaca ttttgcttta cttcattggc tgaaattcca gctggagcta
     1681 gaaatcaaag ccgactacaa cagatacaac tccgatactt aattttttat gccaaagcgc
     1741 ataattcgtt gaccaatttt cattatttct tatctttctt ttcgtttatt tacaagcaga
     1801 ctcaagcgaa atctcattgt gctatatatg ataagactga gaaattcaga aagtcaattc
     1861 attctcatct ctccttatca tactagtctg cttggctagg aagctaatgc tagtattcta
     1921 gaatcttgtc tttcacaatg ctggctatca gatcagacat acctattctt ccgcttcttt
     1981 cagttataag agcaggaaga agctcatagt attcctctgt ttcttcttta atccggatag
     2041 gcagtcaatc ctgaaaaaac agaggcactc ccccttttaa atcttttttc ttacaattca
     2101 gcaagaatcg ctttaagcaa gcctttgaaa tcacctttaa cacgttcagt tacttctaca
     2161 acttcttcgt gattaagttc ttcttggaaa ccagccgcaa agttagtgat acatgaaata
     2221 ccaagaactt tcaaacctga gtgggctgcc acaatcactt caggaactgt agacatacca
     2281 actgcatctg ctccaagtat cttataggcg cgaatctctg ctggtgtttc ataagttgga
     2341 ccagtaacac cgatatagac accttcatca agcttgatac caagtttctt agcaacttca
     2401 tgggcagtag cacgatattc tggagtgtag gatttagaca tatctgggaa acgtggacca
     2461 aagtcatcca agttttcacc catcaatggg ttctgccccg tcatgttgat atggtctgag
     2521 atagccatca aagtaccagg accatatcca ataccaccag ctgcatttgt tacaatgaca
     2581 ccttcacatc cgagaacttt catgacacgt actgggaagg taactacttc cagaggattt
     2641 ccttcgtaga aatggaaacg accttgaaga gccaagacct tgcgacctgc aaggtcacca
     2701 tataccaatt taccagcgtg accgactact gtcgaacgac cccagtttgg aatatctgca
     2761 tagtctacta caactggatt ttcgatttct tctgccaatt ctccaagtcc tgatccaagg
     2821 attagaccga actcaggcgc ttggattcct ttatctttca gaaaagtagc tgtttccttg
     2881 attttatcta aaaatgtcat tgtgtgtctc cttttttagt ttttagcatt tgaccgattt
     2941 tgtcaaaagt tttagcattg cgaatggtga tattgcgata aaaaggcatc ttgagcaagt
     3001 acttatgata ggcagttgca tagtaggatt cctcagagaa tttcccccag aaaatcccaa
     3061 gtcttccaaa atgaaccact tcatcttcca attccaaact gttcactttc tcgatcactt
     3121 gatccacatc caagctctcc gtgtaaaaga ggacatcctt tcgtgccaaa tctttggtcc
     3181 accatttagg tagattttca agttccgctt catagtcttc atgactcagc aacgaaaagc
     3241 tctgaataaa tggataatgg atttcaaaga aagactctaa cttttcaacc aattgggctt
     3301 tgggatctgt cgaagtaaag aaaatattgc cactgttgat gtaggtctca acctttccca
     3361 gtcccaactc tgtcagttct tgacgaagtt gcgccatgac aaccttattt ttcccaccta
     3421 cattaatgcc ccgaacaagt aaagcatatc gcgtcatctt ataccaattt atctaagaaa
     3481 ctttccccaa tcatggcagt ttcaacacca aagttatccg caactgtcgc tgagatatct
     3541 gcaaaatgtc caactggaat gacaccattt cctttaaagg aaggactgta agccaaaagt
     3601 gggatatatt cacgagtgtg gtctgttcct gcataggttg ggtcatttcc atggtccgca
     3661 gtaattaaaa gaagatcatt ctctctcatg gctgcgataa tttcaggcaa gcgttcatca
     3721 aactcatgca agcaatcacg gtaaccatga gcattgcgac ggtggccgta aagggcatca
     3781 aagtcaacca agtttgtgaa ggagaatcct ttttcaaact cagcaaggcc catggtcttc
     3841 aatagtgtat caattccatg gctgtttgac ttgttgtggc ccatgtcatg gttgatacca
     3901 gcaccgttaa agatatcatt gattttacca acagcataag tatcgatacc agcttcattg
     3961 agtttatcga gaacggttgg agcaaatgga gaaacggcta agtcacgacg gtttgctgta
     4021 cgagtgaagt tacctggttc acctacataa gggcgagcaa tgatacgacc tagaagggca
     4081 gggcgctcaa gtgtaattga acgagcgtat tcacagatac ggtacaattc gtccaaagga
     4141 ataatgtctt cgtgggcagc aatttgcaaa acaggatcag ctgaagtata gataatcaac
     4201 tccccagttt ccatttgacg tggtccaaaa tcatcgataa cagctgtacc tgagtaaggt
     4261 ttgttagctt cacgaatgat cttgcgtcct gagaattctt caatttttgt caagatttct
     4321 tctgggaatc cgttccagaa agtatcgaaa ggctcagtaa tgttgagtcc catgatttcc
     4381 cagtgtccag tcatagtatc cttaccaaga gatacttctt ccaattttgt tgcatatcct
     4441 gttggattgc tttccgccgg tacagtcttc aaaggcgttt cgcgaggaat atttcccaga
     4501 ccgattttag ccatgtttgg cacattcaaa ccaactgatt tggaaatgtg tcccagtgtg
     4561 tcagaagctc catctggaac ccctgcattg acaaagttat tagcatctgg tgcagcaccg
     4621 attcctacag aatctagtac caccaagtga atacgattaa attttgacat agtgtatctc
     4681 cttattatgt tgattagttt gcttttgttg aagttaaaat ctgaacccca tctcgtccag
     4741 caacaatgac cttatccacc atttggttga ataaaccgtg ctctacaaca ccaacgacat
     4801 ggtccaattc ttgcccaaag gcaattggat cttcaatgac atccaaagca aggtcaatga
     4861 taaaattctg catatcggtc acaaaacgtt ggccgtcttt ttcacggaaa cttggtttgt
     4921 agccagctcg ctcaaaacga cgaaagacct gttctgcgcc atactgaacc acttctactg
     4981 gcaatttaaa agcacccagt ttctcgacta gcttgctttc atccaccacc caaatgtatt
     5041 cttttgaggg cgttgccaca accttctcca tcagaagggc accaccaccg cctttgattc
     5101 cgttaaactg actatccact tcatccgccc cgtcgactgt cacatcgaca aagtctactt
     5161 ggtcaataga cttgagcggg atattgagcc cttcagcctg tttactggtc acactagaag
     5221 tcgtcacagc tgtaatctgc aagccttctt ccttgattcg acgaccgatt tcttcgacaa
     5281 aataataggc agtagaacct gttcctagcc ctacaaccat cccatccttg ataaattcag
     5341 cagccgtgat acctgccatt ttcttcagat tttccactca aacacctcca ttaaagagct
     5401 acctctatta taccatgtaa aggcttttat ttcatctaaa agtaccttta gaatactgct
     5461 tatttatcct tgaaattgaa tttttttaat aaaagtagag ctctaacaaa tcaatttaag
     5521 tacggatgta aaccaaatca actctaacaa aaatagattt acaagagtaa aaaaacgaac
     5581 aaatttagtt tcttagcaag aagaatacta aacgttcgtt ttttgtgaat taagaataat
     5641 gatacaaatt atcaacattt tttgtctaac tattttagac atatcttttc attactttag
     5701 gaagacaatt actaatttcc gtcggcaaga ccacataatt ttcttgagat agttcttggg
     5761 ctatggctga atgaagatgg gttgctactg ccacacgttc gtagagactg gcctgtcgaa
     5821 attggcctgc aaatcctgca atcattccag ccagtgtatc acccatacca ccagtcgcct
     5881 gatagggacc gccaaccttt aactggtaat aatcagactg gccaacttgc caaatacgag
     5941 tagctggacc tttctctacc aaaattgttc cttgagggaa agaagtcagg gcactagatg
     6001 ttgtaccttc gttttgcttt tcaatagcaa taccagacag tttttcccat tctttttggt
     6061 ggggagttaa gataagctgg ttagatggaa acaacaaact tgtcctagca aggatggtta
     6121 aggcccctcc atctacaatc aaaatctgat tcttttttaa gctagcaaag acctgtttta
     6181 caagattttc tccaaacgta tcgtctcgta aaccaggccc cagcaagaca acttctgcct
     6241 tctccaattg ctcttgtaac aattgctgat cttgcagaga aaaggccata gcctcaggca
     6301 aatggctgtg tagagcaggg atattttccc tgtccgttcc aacggttacc aatcctgcac
     6361 cgctttttac agctgctaaa gcagccatga tgatggcacc accataagga taagtcccac
     6421 caagcaacag cagacgaccg tagtctcctt tatgacttgt acaagaacgt tcaataatga
     6481 ctttttctag taaggtttga tcaatcactt tcatcgtttt ttcctcaact atcctttatc
     6541 tgattgtttt aaaacaactt gatagacttt cttagtccac tcttctaagt cttctccagc
     6601 atagtcaaga taatacaccc tatcttgtat tttggttgga ataatatttt caaattggct
     6661 cttattatga gttggaataa tgcaaatata caactcactt aataaattaa gcatgtcctt
     6721 aatttttaca gcatggtaac catcaaaaat ataaccagga actttaatca aatctgtata
     6781 gataaaatga aaacttactc caggaataaa cttgtcttgt aattctttct ctgaaggagc
     6841 tcgacctaat aaacgctcca tcgccaatcg ataaccagaa cttgtatttg accaccctaa
     6901 catgacatag tcaaaataat ctatcggatc agatgcagca tttcgactat ccaaaaccaa
     6961 ctcctcagca tctcgcccaa aggctttcac agcagataaa agacgaccag ttttaaaaat
     7021 agaaatcgct gcatctatag tcgtagtatg actacagtag tctgaagtca ctaacctctt
     7081 atgtaggtca taagtcgagg ctactctttt atctgtagga gtaaatggtg gcagaaaggg
     7141 atgctgacag atgtaatcta aaatttcttt ttttaaacaa gaatcagcac actcaatact
     7201 tgtttgacgt ttatgaagtt tttcgtaagc tataaattta gcaatttttt ctaattccca
     7261 ctcttgaata tttggataat caattagtgc taagtagtat attccatccc attgtaaact
     7321 atattcccca ttataaattt ttaaaatcat ctaatcacct cactccatta tacaacaaaa
     7381 aggaggcgca aacctccttt tataaactta tttctaaaat ttaatattca tttctatcat
     7441 tttagatata gctatagaaa atacactttc ctcagaaaat ttttatctta ttttctatcc
     7501 aatgtcctaa gtgctgcctg ataggtttgc tccatcagca tagtaatggt cataggaccg
     7561 acacctccag ggactggcgt aatgtggcta gcaagtgggg caaccgcctc ataatcaaca
     7621 tccccacaga gcttaccatt ttcatcgcgg ttcatcccaa cgtcaatgac taccgcacct
     7681 ggtttgacaa agtcagcagt cacaaacttg gcacgaccga ttgcaacaac cagaatatct
     7741 gcttttgcag ccaccttgga aagattatga gtacgtgagt gagtcaaggt tactgttgca
     7801 ttctttgcca aaagaagctg ggccataggt tttccgacaa tattggatcg accgatgacg
     7861 actgcatttt taccttccaa gtcaatccca tattcatgga acatttccat aattcctgca
     7921 ggtgtcgagg gaatcatgac tggatgtcca gaccaaagac gtcccatgtt taggggatgg
     7981 aaaccatcca catccttttc tgggtcaatg gctaataaaa ccgcctcttc atcgatatgt
     8041 tttggtaatg gcaactggac caaaatccca tgccaagctg gatcctgatt atatttagca
     8101 atcaggtcta acaattcctc ttgagtaatg gtctctggaa ctcgcactac ttcgctacgg
     8161 aaaccagctg caagagctga cctctccttg ttgcgaacgt agacttggct ggctggattg
     8221 tccccaacca aaatcactac caaaccaggc actagacctg tttcttcctt taatttttca
     8281 gtcttttcag ccaactgccc ctgcaatttg gccgctaaag cttttccatc aataatctgt
     8341 gtcatatcac ttctcttttc tttcaaatat cttccattat accaaaaact gccagcaccc
     8401 tctaacactt ctttcctagg caatcctata gtttcaaact ataagaaaaa gctctggttg
     8461 agcttttcac taatcttgtc ttgaaatttt aaaatagatt ataaaacctt actcaagaag
     8521 tctttagtcc gttgttcttg ggtttgttca aaaatctgtt ctggtgttcc gtcttcaaca
     8581 accacaccgt ctgccataaa gatgacacga tctgccacct cacgggcaaa tcccatctca
     8641 tgtgttacga taaccatggt cattcctgac ttggctagat cttgcataac agccagaact
     8701 tctccaacca tctcaggatc tagtgctgaa gttggctcgt caaagagcaa aacatctggt
     8761 tccatagcca acccacgcgc gatggcaatc cgttgttgtt gcccacccga cagactctgt
     8821 ggataagcgt ctgccttatc tggcaaacca actttttcca aaagctcttg ggctctcttc
     8881 tcagcaactt ccctgctttc acctttagtc ttgataggtg acaaggtaat attttccatc
     8941 acagtcatat taggaaagag gttaaattgt tggaaaacca tgcccatctt ctcacgcatg
     9001 gcaaacaggt cattcttctt gtccgtaata tcgactccct caaagataac cttccccttg
     9061 gttgcttcct ccaacaaatt catagagcga agcaaggtag atttcccact ccctgaagga
     9121 ccgatgataa cgacaacttc tcctctttta atctcgaggt tgatgccctt caatacttca
     9181 ttctttccaa aagatttatg taaattttca atttttatca aggtttctgt cattatttct
     9241 tatctccttg tcccatatgt ttttcaaaag ctttcaaggc tactgtcaga atagaggtca
     9301 taatcaagta gtaaaatgct gcaaataaaa gtggtgttaa aggtagatag gttgttgtag
     9361 aaactgttgt agccccattc cacaactcca tgaccccaat agctgataag agggagctgt
     9421 ccttgataat ggtgataaat tcgttcccca atgctggcaa gatatttttg actgcttgtg
     9481 gcaaaatcac ataacgcatc gcatttttag gacgaatccc tagcgaataa gccgcctcta
     9541 gctgaccttt tggaaccgca ttgattccgg cacgaacaat ctccgaaaca taagcaccac
     9601 tattcataga gataatcaaa atccctggaa tcagacgcga aaaatcaaca cctaaaattc
     9661 caatctgaat agtcggagca ttgatatgca taagagcaaa ggcaatcata atttgaacca
     9721 tcatcggtgt cccacggaaa atccaaacgt acaagttggc caaccaaaca agcggtttaa
     9781 actttgaacg ttgcccaaaa gccaagacaa cacccaaaat agttcccaaa aagataacac
     9841 agatagaaat aagaatcgtc acaacagccc catagttaaa ataaggtaaa tacttaggta
     9901 aaaaagaaaa attcatagtc acactgcttt ctaaaataga atactgtatg attataacat
     9961 gttttgaatt aaaatttaaa ttttttatcc aatttattta aaaatcttta aactagcaga
    10021 attagcgaga aatcttagtt ttttctagtc aagttggcct cctttatggt aaaatagtaa
    10081 cgataagaaa tgaaggagaa tcaaaatgga atcacatttg gttagaatca tcaaccgcct
    10141 tgaagcaatg gcaaacgatg gcggaaattt aaaacgtaat tttgaacgcg aaggagttgt
    10201 tgttgcagaa gttgcgtaca gccacgacga agaaaacggc ccaatcttta cccttcgtga
    10261 cgtagaagat cgcgaaacgt atacgtttga tagcattgac ttaatcgcaa tggaaattta
    10321 cgaacttctc tactaatcta aaacaagctg ggattgtccc tgcatgtcta agtaaaatcc
    10381 tttttgtctc acaaatagga tttttttata gtccaaattc acaaaaattt tcattgtaac
    10441 aacttctcaa agcttcaatc tttttacttg ctttacaccc caatcctgtt ataatgagag
    10501 tatagaattt gactattttt gacctttaaa caagtcaggc taaagaaaga aatgaggtag
    10561 atgtatgctt tgtcaaaact gtaaaattaa cgactcaaca attcatcttt acaccaatct
    10621 caatggaaaa caaaaacaaa ttgacctctg tcaaaactgc tataagatta tcaaaacaga
    10681 tcctaacaat agcctcttca aaggtatgac ggatctgaac aatcgtgact tcgatccctt
    10741 tggtgatttc ttcaatgatc taaacaattt cagaccttct agcaatactc ctcctattcc
    10801 cccaacccaa tcaggtggag gttacggtgg aaacggcggt tatggttccc aaaatcgtgg
    10861 atctgctcaa actccgccac ctagccaaga aaaaggcctg ctggaagaat ttggtattaa
    10921 tgtaactgaa attgcccgtc gtggagacat tgaccccgtt attgggcgcg acgatgagat
    10981 tatccgtgtc atcgagattc tcaatcgtag aaccaagaat aatcctgtcc ttatcggtga
    11041 acctggtgtc ggaaaaacgg ccgttgtcga aggtctagct cagaaaattg tcgatggcga
    11101 tgtgccacat aaactccaag gtaaacaagt catccgtctg gatgtggtta gcttagttca
    11161 aggaacgggg attcgaggac aatttgaaga acgcatgcaa aaactcatgg aagaaattcg
    11221 caaacgtgaa gacatcatcc tctttatcga tgaaatccat gaaattgttg gtgctggttc
    11281 tgcgagtgat ggtaatatgg acgcaggaaa tatcctcaag ccagcccttg ctcgtggaga
    11341 actgcaacta gtcggtgcta ctaccctcaa tgaataccgt atcattgaaa aggatgctgc
    11401 cctcgagcgt cgtatgcagc ctgttaaagt cgatgaacca acggtggacg aaacaatcac
    11461 tattctcaaa gggattcaaa agaaatacga agattaccac cacgttcaat atacagatgc
    11521 tgcgattaaa gcagctgcaa ctctttccaa tcgctacatc caagatcgct tcttgcctga
    11581 caaggccatt gacctcctag atgaagctgg ttctaagatg aacttgacct tgaattttgt
    11641 ggatcctaaa gtaattgatc agcgcttgat tgaggctgaa aatctcaagt ctcaagctac
    11701 acgagaagaa gattttgaga aggcggccta cttccgcgac cagattgcca agtataagga
    11761 aatgcaaaag aaaaagatca cagaccagga tactcctatc atcagcgaga aaactattga
    11821 gcacattatc gagcagaaaa ccaatatccc tgttggtgat ttgaaagaga aagaacaatc
    11881 tcaactcatc catctagccg aagatctcaa gtctcatgtt attggccaag atgatgcagt
    11941 cgataagatt gccaaggcta ttcgccgtaa tcgtgtcgga cttggtaccc ctaaccgccc
    12001 aatcggaagc ttcctcttcg ttgggccaac tggtgtcggt aagacagaac tttccaaaca
    12061 actggctatc gaactttttg gttctgctga tagtatgatt cgctttgata tgagtgaata
    12121 catggaaaaa catagtgtgg ctaagttggt cggcgctcct ccaggttatg ttggctatga
    12181 tgaggctggt caattaactg aaaaagttcg ccacaatcca tattctctca tccttctcga
    12241 tgaagtggaa aaagctcacc cagatgttat gcacatgttt cttcaagtct tggacgatgg
    12301 tcgtttgaca gacgggcaag gacgcaccgt tagcttcaag gatgccatca ttatcatgac
    12361 ctcaaatgca ggtacaggaa agaccgaagc tagcgttgga tttggtgctg ctagagaagg
    12421 acgtaccaat tctgtcctcg gtgaactcgg taacttcttt agcccagagt ttatgaaccg
    12481 ttttgatggc attatcgaat ttaaggctct cagcaaggat aacctccttc agattgtcga
    12541 gctcatgcta gcagatgtta acaagcgcct ctctagcaac aacattcgtt tggatgtaac
    12601 tgacaaggtc aaggaaaagt tggttgacct aggttatgat ccaaaaatgg gagcacgccc
    12661 acttcgtcgg actattcaag actatattga ggacacaatc actgactact accttgaaaa
    12721 tccaagcgaa aaagatctca aagcagttat gactagcaag ggaaacattc agattaaatc
    12781 tgccaaaaaa gctgaagtta aaagttctga aaaagaaaaa taaatcctat agaaaaggag
    12841 tagaaaatga aatttttctg cttctttttt tactaaaata actgtaattt cttgacagct
    12901 tgccctttgt ccattatgat atatagtaga ctgaatctga aatagtacga aacaattgct
    12961 aaaacattta tagaaattaa ttttactttc ccaatcgatt tgttctcatc ttatttcaat
    13021 ctgctatagt caattgaaac aagagcagga caaaagagcc tcgtaaaagg tattgcaaca
    13081 acttggtaat acctttttga ggtgcttttt gatatgagcc catgttttct caataggatt
    13141 gtactcaggt gagtagggag gaagaggtaa aagtttatgc ccaaactctt cgcataaaag
    13201 ttctagcttc cccattctat ggaatcttgc attatccata ataataaccg atggtgtggt
    13261 taatgttggt aagagaaact tc
//
LOCUS       DAVBGY010000052        13121 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVBGY010000052 DAVBGY010000000
VERSION     DAVBGY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13121
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.052"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            1..189
                     /locus_tag="VQ620_001606"
     CDS             1..189
                     /locus_tag="VQ620_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764200.1"
                     /translation="MIFTKQIRHWILSDQVISGKRKLFFREDTPKEILDLYENIKSKL
                     DFAYQEVHSNNGLKKYEK"
     gene            354..533
                     /locus_tag="VQ620_001607"
     CDS             354..533
                     /locus_tag="VQ620_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764201.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            676..849
                     /locus_tag="VQ620_001608"
     CDS             676..849
                     /locus_tag="VQ620_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HET2764202.1"
                     /translation="MTQKEMVKLLTAHGWIKTRGGKGSHIKIEKQGERPITILHGELN
                     KYTERGIGKQAGL"
     gene            886..1338
                     /locus_tag="VQ620_001609"
     CDS             886..1338
                     /locus_tag="VQ620_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET2764203.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            1629..2099
                     /locus_tag="VQ620_001610"
     CDS             1629..2099
                     /locus_tag="VQ620_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HET2764204.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            2118..3206
                     /locus_tag="VQ620_001611"
     CDS             2118..3206
                     /locus_tag="VQ620_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET2764205.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTRKSGRFHLSRTAIMKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAILFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVRKDVVQTHKAMKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            3187..3345
                     /locus_tag="VQ620_001612"
     CDS             3187..3345
                     /locus_tag="VQ620_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-dihydro-8-oxoguanine-triphosphatase"
                     /protein_id="HET2764206.1"
                     /translation="MELEISDFPGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     KSVRENLI"
     gene            3455..4489
                     /locus_tag="VQ620_001613"
     CDS             3455..4489
                     /locus_tag="VQ620_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET2764207.1"
                     /translation="MKKSDVLDLIKYHYEGRETEFRNQSIAIARNFNKHGDTQIAQYI
                     MGLMSQSDRFMPQIENPSEYLTPAKLDIGPLPLPLSIMNDLKGIINAVNHHIGINKFL
                     FVGSPGTGKTESVKQVARLIGKELLVVDFSHLVDSKLGQTVKNLATLFNEINNLPFKQ
                     NYIILFDEIDSIVLDRVNQNDLREMGRVTSAFLKELDRLSPEIVLIATTNLFENLDKA
                     VTRRFDAIIDFDRYTDEDKVEVATIILNELLKQFKNVARDLKLFKKIINSANVIPNPG
                     DLRNSIRTSLAFSDPSDPHDYQKRLLRSLHNGRNLSISKLSKLGFTVREIEILTGISK
                     SSVSRELSED"
     gene            4491..6734
                     /locus_tag="VQ620_001614"
     CDS             4491..6734
                     /locus_tag="VQ620_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET2764208.1"
                     /translation="MNTLLTLRGKSFTPKSAKGRGPITIPADSIVTLNHLNELYTSLE
                     RVKEYWSNNTIIDGVLISVYYNRVLAKSNRIDGFFNVGGGNAIPNETIVGAKFSNDKS
                     RHIITHYISKEVLDKTIEHTKKVITIFKDYFASAPMTSMVFSDSSKFNQIDYSKYGLS
                     KSKFKQYLRDACFVKGFGIEQADMSTIRNSIVTIYDVKTNLLTLLKQLDIDVSEANIM
                     DQTTVLLDEKNIELLLLKAPYLVSMIVEDFSSLSPENFYNDGNTFYNNLPFPTNEPII
                     GVIDTLFDDRVYFNEWVEYHDLISPDISKGSQDYKHGTAVSSVIVDGASLNPKLDDGC
                     GRFRVRHFGISLQSGFNSFTIIKQIREIVSQNPDIKVWNLSLGSNDEIRENFISVEGS
                     LLDEIQFDNDVIFIIAGTNGIAPDGQRKKIGAPADSLNSIIVNSVDFSNQVVSYSREG
                     VVLSFFIKPDVSYYGGGNGDFINVCEPLGLARVAGTSFAAPFIARKMAYLIHIMGLSR
                     EEAKALLIDSAIPWNDKKTFADISLLGYGVVPIKIEDILSTPDDEIKFIVSDVSRAYE
                     TYNYDFPVPISKEHYPYVAKATMCYFPKCSRNQGVDYTNTEMQLSLGVLDKKGIKTIN
                     NDNQYSENAPGYVREKSARNIFRKWDNVKHLGEKFSSRKKAKPILNPSNPQWGMSVKT
                     IERLRNDDGYGIRFGVVVTLKELNGVNRIEDFIQQAELRGWLVNRLQVEAQVDLFNSL
                     NEEIEFE"
     gene            6785..7804
                     /locus_tag="VQ620_001615"
     CDS             6785..7804
                     /locus_tag="VQ620_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET2764209.1"
                     /translation="MNITYIVGNGLDLQYGLKTRYKDFYEFQNRTYISRKENEEEYSN
                     LIYESLFSDTVNDYENWSDFELSIGKLTKDNDLISSSIEIKEKFIDDFSEVVDDLREY
                     LRIQQEKNLEKGNAIDFISTLDNMRTSLPVINQPAIDKKYNENLYQHDIVNILTLNYT
                     NVIDKLYNESAKSFSNQLRTNNYKFHIAPPIHAHGTVDICTVLGVSDETQISNSFDEE
                     QKESLIKNLVLKNYRENMDVKNSDIIKNSDIIILYGVSLGETDGYIWNQIAEQSIRSS
                     VPVIIYHYVPHFDAGNPTRVKRLYRNVEDKFIQNSGIDLELEKKLRDNLIVVIGKTIF
                     NLMER"
     gene            7817..8767
                     /locus_tag="VQ620_001616"
     CDS             7817..8767
                     /locus_tag="VQ620_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET2764210.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSLDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPETVDVAAVEADLQARLAELTDFMDLGEVKMGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNTG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDKW
                     DMVRQSAESAGFFLETHMIQGEWNACVFKKTKDISGVIGG"
     gene            8769..9512
                     /locus_tag="VQ620_001617"
     CDS             8769..9512
                     /locus_tag="VQ620_001617"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET2764211.1"
                     /translation="MQQYFVKGSAVSPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSVIVAYEESGKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            9523..11319
                     /gene="pepF"
                     /locus_tag="VQ620_001618"
     CDS             9523..11319
                     /gene="pepF"
                     /locus_tag="VQ620_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET2764212.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETA
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEATFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIISYSAELGE"
     gene            11565..12119
                     /locus_tag="VQ620_001619"
     CDS             11565..12119
                     /locus_tag="VQ620_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HET2764213.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRDDLSNQGL
                     HYLSKEKDTTGFSSQYGWVHSFAHGADLLTEVVCHPDFPKNRVHEVFDILGQLFKRMS
                     IRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDDLKEAIQSFQY"
     gene            12230..12931
                     /locus_tag="VQ620_001620"
     CDS             12230..12931
                     /locus_tag="VQ620_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET2764214.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLGN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEELLKLKKPCSSS"
     gene            complement(12923..>13121)
                     /locus_tag="VQ620_001621"
     CDS             complement(12923..>13121)
                     /locus_tag="VQ620_001621"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764215.1"
                     /translation="DETDEVSLKHCFEVADETDEVSLKHCFEVVDETDEVSNHTYGKV
                     KLTWFEEIFEEYHTKKPYSSR"
BASE COUNT         4104 a         2187 c         2879 g         3951 t
ORIGIN      
        1 ttgattttta caaaacaaat tcgtcattgg attcttagtg atcaagttat ttcagggaaa
       61 agaaaacttt tcttccggga agatactcca aaagagattt tagatttgta cgaaaacatt
      121 aaatctaaac ttgattttgc ttaccaagaa gttcattcta ataatgggtt gaaaaaatat
      181 gaaaaataga ataattgatg tttttgaagt agtaaatcgc ctccttgtaa taactgttga
      241 aaatcctgat ttcgaagact tgagagttaa tcatactcaa tgaaaatcaa agagcaaact
      301 aggaaactag ccgcaggctg tacttgagta cggtaaggcg acgttgacgt ggtttgaatt
      361 tgattttcga agagtatcag tttgtaaaaa ttggagataa aaaatataga gtacgcagtg
      421 gtcctatgat tcattcaact ccgcctcaat ctgtcttaga tcgagatact tttacaattg
      481 actatacaga tgatgaattg ctcgataaag aagcagtttt tactactcat tagaaaaata
      541 ggtctgaata aattggtttc ccttagaacc acatctaggg gcttttatgt ataatagaat
      601 aataagtatc actcctttac aatacgtgta acacgtgtta taatggtgtt gtcaggaggt
      661 aaaagcgcta tacctatgac acaaaaagag atggttaaac tccttacggc ccatggttgg
      721 ataaagacta gaggcggtaa aggctcccat attaaaatcg agaagcaagg ggaaagacct
      781 atcacaatcc ttcatggtga gctgaataag tacactgaaa gagggatagg aaagcaagct
      841 gggttgtaaa attcagtccc tgctttttga tatggaggta gtgaaatgtt agttacgtat
      901 ccagctttat tttattatga cgacacagac ggaacagaag cgacttattt tgtccatttc
      961 ccagattttg aatactcagc tacacaagga gaggggattt ctgaggcttt ggctatgggg
     1021 tcggagtggc tagggataac tgttgcagat ttgatagaga gtgatggaga gttacctcag
     1081 ccatcagata tcaatagctt gtctttaatt gataatgatc cttttaaaga tgatgaagat
     1141 ttcgtgtcaa cctatgacct tgataaatct tttatttcta tggtatctgt tgatgtatca
     1201 gaatacctag gaagtcagga acccattaaa aagactttga ccataccaaa atgggcagat
     1261 aagttgggac gagaaatggg acttaacttt tctcagactt tgacagacgc tattgcagat
     1321 aagaaagttc aagcctaaga ttaagcgtgg tatcgtggta atagtcaata aaaaagcacg
     1381 tccacttgta ctagttactc gcctattgat tttatagatt tattagccct ttcgagggct
     1441 ttttatattg atttttatga gaaatgaaga aatcagactt ttaagaaaat cattgatacc
     1501 aagggtttaa atgaggtaat atggtataat taggacataa aataattttg tggtaagatg
     1561 gtagtatcta ttttagcata tttccgagca atcggggcga ttaaagagtc gcatagaaag
     1621 aggacaaaat ggcaacatac ggatttttag atattttaga ggaagagttg gacaagaact
     1681 ttccctttga ctttgagatt agctgggaca agcgtaacca cgcggtagaa gtgagttttc
     1741 tattggaagc gcaaaatgct gcaggtgtgg agatggtgga tgaagacgga gaggtttcat
     1801 cagatgacat tctttttgag gaagcggtcc ttttctacaa tcctgctaag tcaaccgtca
     1861 atgcagaaga ctatttaacg gttatccctt atctacctaa aaaaggattt tctcgcgagt
     1921 ttttagctta ttttgccctt ttccttaaag atactgctga ggttgggcta gatgtcctca
     1981 tggacttttt ggaagatcca gaagcagagg aatttgttat ggaatggaat caagaagtct
     2041 ttgaagaagg aaaagttggc ttggaagagg gagaatttta tccttatcca agatactagg
     2101 agttggttgg aggtgttatg aagaaaattg ggacttattt ggtttatgtg ctagctgttg
     2161 tctttattat gctagctttt gcttgtggaa caatcgcatt tgcagagttg ggatattccg
     2221 cagttttagc ctttactttt ggttatgcct tcgctcttct aagcatgtat ttaatcttta
     2281 ttcttcatga gctgggtcat gctttttgtg gctacttgac aggctatcgg ctggtggctt
     2341 ttggattagg acattttatt ttgacaagaa agtcaggcag gtttcatctt agtagaacag
     2401 ccattatgaa aaatgttggt gctcaataca ttggtttaaa agaggatgaa agcgatcaaa
     2461 gaatcatcct gatgctttca ggaggcttga tggttcatct cggcttgata ttattggcga
     2521 tattgtttgg atttttgaca agaagctggt attttgcagg gacttggatt tttcttaatt
     2581 tatctttctt cctaaataac attttaccag tcgacatcac cgatggagca aaaatttggg
     2641 aattactaca acaccctgaa aataccaaat acgcctacct ggtattgaga cattctgccc
     2701 agaccttgct ggctcctcaa gaatatgatt tgaaagactt tgttcaagca gttcctgagg
     2761 atgcacgagg gagttttgcg gatggtgtat taggtatgca aggtgaagtt tccattttgg
     2821 aagggaagga agaagtagct aaacagcagt ttcaagcatt acttgagagg acaactactc
     2881 cgatgatgca gacagttgcc caactatccc tgcttcatat cgccttgttg gaaggagatt
     2941 ttgagcaagc ggagcaatat gccagtattc gacaggtcaa atcgtttctg tctctcaaac
     3001 tgtccaatct tcaagtgata caagcctggt atcaatttaa ggtaaggaaa gatgtggttc
     3061 aaactcacaa ggctatgaag attgctagac agaaaatgga tagtagtcga atgttgaggg
     3121 acgagaaacg ctattatgaa aacttgttag cagagctgga aaaggaaatg ttagaaggaa
     3181 ctgaagatgg aactagaaat ttctgatttc ccaggctgta agattgcttt gttttgtggg
     3241 gataagcttt tgactatctt acgcgatgat aaggcaagca ttccctgggc caatatgtgg
     3301 gaactgccag gtgggggaaa gagtgtcagg gaaaatctga tatagttttg cattaaaaga
     3361 atatcctttc aaggttattc ttttttaatt ttgtcccaaa ataattgact ttgtcccgga
     3421 aatatagtat tatttgtata aggaggaatg aatcatgaaa aaatcagatg ttcttgattt
     3481 aataaagtat cattatgagg gcagagagac agaatttaga aatcaatcca tagcaattgc
     3541 tcggaacttc aataaacatg gtgatacaca aatagcacaa tatattatgg ggttaatgtc
     3601 tcaaagtgat agattcatgc cacaaataga aaatcctagt gagtatttga ctcctgctaa
     3661 actagatatt ggtcctctcc cattgcctct ttcaattatg aatgatttaa aaggaataat
     3721 aaacgctgtc aatcatcata ttggaataaa taaattttta tttgtgggat ctccaggtac
     3781 tggaaaaact gaaagtgtaa agcaggtggc tagacttatt ggcaaggaat tacttgtagt
     3841 agatttcagt cacttagtag atagtaagct agggcaaaca gtcaaaaact tagcaactct
     3901 ttttaatgaa atcaataatc ttcctttcaa gcaaaattat atcatcctat ttgatgaaat
     3961 tgattccata gtgttggata gggttaatca gaatgattta agagaaatgg gacgagtgac
     4021 ttctgccttt ttaaaggagt tggataggct gtcaccagaa attgtattaa ttgcaacaac
     4081 aaatcttttt gaaaatcttg ataaagcagt tacgagaaga ttcgatgcca taattgactt
     4141 tgaccgttat actgatgaag ataaagtaga agtagctact attattttaa atgagttatt
     4201 aaagcaattt aagaatgtag ctagagattt aaaattattt aaaaaaatta tcaatagtgc
     4261 caatgtaata ccgaatcctg gtgatttgag aaactcaata agaacgtcat tggcatttag
     4321 tgacccatca gatccgcatg attaccaaaa acgcctgcta agaagtttgc ataatggtag
     4381 aaatttatct atttctaaat tatcgaagct aggttttact gttagagaaa ttgagatttt
     4441 gacaggtatt tctaagagta gtgtatcacg agagttaagc gaggattaaa atgaatacat
     4501 tattgacatt acgaggtaaa agttttacac caaagtcagc aaaggggagg ggaccgataa
     4561 cgattcccgc agattctatt gttacgctaa accatttaaa tgaactttat acatcccttg
     4621 aacgagtaaa agaatattgg tctaataaca caatcattga tggagtactt attagtgtgt
     4681 attataatcg tgtcctagct aagagcaatc gtatcgatgg tttttttaat gtgggtggcg
     4741 gaaatgcaat tccaaatgaa acgattgtgg gtgcaaaatt tagtaatgat aagagtaggc
     4801 atattattac acactacatt tcaaaggaag tgttagataa gacaattgag catactaaaa
     4861 aagttataac aatattcaaa gattattttg ctagtgctcc catgacgagt atggtgttct
     4921 ccgactcttc aaaatttaat caaattgatt attctaagta tggtctgtcg aaatcaaaat
     4981 tcaaacaata cttaagagac gcttgctttg tcaaaggatt tggtattgaa caggcagata
     5041 tgtctactat acgtaattct attgtaacaa tctacgatgt aaaaactaat ctcttaacat
     5101 tacttaaaca acttgatatt gatgtttcag aagctaacat catggaccag acaactgttt
     5161 tacttgacga aaaaaatatt gagttactac tgctaaaagc accttatcta gtatcaatga
     5221 tagtagaaga tttttctagc ctgtcaccag agaattttta taatgatggg aacacttttt
     5281 ataataatct cccttttcca actaatgaac ctattattgg tgtcatcgat acgttgtttg
     5341 atgatagagt ttatttcaat gagtgggtag agtatcatga tttaatttca ccagatatta
     5401 gcaaaggaag tcaagactat aagcacggta cagcagtttc ctcggtaata gtagatggag
     5461 ctagtttgaa tccaaaacta gatgacggat gtggaagatt tagagtcaga cattttggga
     5521 tatctttaca gagtggtttc aattccttca caattatcaa acagattaga gaaattgtgt
     5581 ctcaaaatcc tgatattaag gtttggaacc tttcgcttgg atcaaatgat gaaattaggg
     5641 aaaactttat ttctgtcgag ggttcgttgt tagacgagat tcaatttgat aacgatgtaa
     5701 tttttattat tgcaggcact aatgggattg cccctgatgg acaacgaaaa aagattggag
     5761 caccagcaga ttcactcaat tcaattattg taaattctgt tgattttagc aatcaagtcg
     5821 tttcttattc acgcgaagga gttgtcctgt ctttctttat caaacctgat gtttcatact
     5881 atggcggggg gaacggagac ttcataaatg tttgtgaacc actagggctg gctcgagtag
     5941 ctgggaccag ttttgcggct ccatttattg ctagaaaaat ggcttacctc attcatatta
     6001 tgggattaag tcgtgaagaa gcaaaggctt tattaattga ttcagcgata ccatggaatg
     6061 ataaaaaaac attcgcagac atttctttac ttggatatgg ggttgtgcca atcaaaatag
     6121 aagatatttt gtctacacct gatgatgaaa ttaaatttat agtctcagat gtttctagag
     6181 catatgaaac ttataattat gatttcccag ttccaatttc taaagaacac tatccatatg
     6241 tagcaaaggc cactatgtgc tattttccaa agtgttctag aaatcaagga gttgactata
     6301 ctaatacgga aatgcaactt agtttgggag ttttagataa gaaaggcatt aaaacaatta
     6361 ataacgacaa tcaatattct gaaaatgcac caggatatgt gagagaaaag tcggcacgaa
     6421 atatttttag gaaatgggat aatgtcaaac atttaggaga aaaattttct tctcgtaaaa
     6481 aagctaagcc gattctgaat ccttctaatc ctcaatgggg aatgagtgta aaaacaattg
     6541 aacggttaag aaatgatgat ggttatggta ttcgttttgg agtagttgtt actctaaagg
     6601 agttgaatgg tgtcaataga atcgaagatt ttattcaaca agccgaatta cgcggttggc
     6661 tagttaatag attgcaagta gaagctcaag tagacctctt taatagttta aatgaagaaa
     6721 ttgaatttga atagtggata ttttatcctt attctagatc ttaggaacac aagagggcaa
     6781 agatatgaat attacttata tagttggaaa tggtttggat ttgcaatatg gtttgaaaac
     6841 aagatataaa gatttctatg aatttcaaaa taggacttac ataagtagga aagagaacga
     6901 ggaagaatat tcaaatctca tttatgagtc tctattttct gatacagtaa atgattatga
     6961 aaattggtca gattttgaat tgtcaattgg taaattaaca aaagataacg acctcatctc
     7021 ctcaagtata gaaataaagg agaaatttat agatgatttt agtgaagtag ttgatgattt
     7081 aagagaatat ctacgcatac aacaagaaaa aaatcttgag aaaggtaatg caattgactt
     7141 tataagcact ctagataata tgaggacctc tctccctgta attaatcaac ctgctatcga
     7201 taaaaaatat aatgaaaatc tatatcaaca cgatatcgtg aatatattaa ctcttaatta
     7261 cacaaatgta atcgataaat tatataatga gtcagcaaaa tcatttagca atcaattacg
     7321 aactaataat tataaatttc acattgcgcc tccaattcat gcacatggaa cggtagatat
     7381 ttgcacagtt ttaggagtta gtgacgagac acagatttca aatagttttg atgaagaaca
     7441 aaaagaatcc ttaataaaaa atttagtttt gaagaactat agagaaaata tggatgttaa
     7501 aaatagcgat attataaaaa attctgacat cataattctt tatggggttt ctttgggaga
     7561 gactgatggg tatatctgga atcagattgc tgaacaatca atcagaagtt ctgttcctgt
     7621 cataatttac cattatgtac ctcattttga tgcaggaaac cccactagag taaaacgctt
     7681 atatagaaat gtagaagaca aattcataca aaatagtgga attgatttag agttagagaa
     7741 gaaattaaga gataacctta ttgttgttat tggaaagact atttttaatc tgatggaaag
     7801 gtaagcaaaa tacatagtgg aaacatggca agagttaaaa gttacagtga agcgtgaggg
     7861 agaggagttg gtttccaatc tcttgattga gctgggagcg caaggtgttg cgattgaaga
     7921 cagtctggac tatgtgggaa atgttgaccg cttcggtgaa attttcccag aggtggagca
     7981 acaggaagaa atcgtagtga ccgcctacta ccctgaaacg gttgatgtag cagcagttga
     8041 agcggatttg caggctcgtc tggcagaatt gaccgacttt atggacctag gagaagtcaa
     8101 gatgggcacg actgccttgg ctgaggaaga ctgggctgac aactggaaga agtattatga
     8161 accggcacgc atcacccatg acctgaccat cgtaccgtca tggacggact atgaggcaac
     8221 agcaggagaa aagattatca agctggatcc tggtatggcc tttgggacag ggacccaccc
     8281 aacaaccaag atgagcctct ttgccttgga acaggttctt cgtggtggcg aaacagtgct
     8341 agatgtaggg actggttcag gggttctttc tattgcaagc tcactcctag gtgccaagga
     8401 gattttcgcc tatgacttgg atgatgtggc ggttcgtgtc gctcaggaaa atattgagct
     8461 caacactggt atggaaaata tccatgtagc agcaggagac ttgcttaagg gagttgagat
     8521 tgaagcagat gtcatcgtgg ctaatatctt ggcggatatt ctcattcatc tgacggagga
     8581 tgcttatcgc ttggtaaagg atgaaggcta cctcatcatg agtggcatta tcaaggacaa
     8641 gtgggacatg gtgcgccagt cggctgagtc agctggattt ttccttgaaa cccatatgat
     8701 tcaaggggaa tggaatgcct gcgtcttcaa gaagaccaag gatatttcag gtgtgattgg
     8761 aggctaggat gcaacagtat tttgtcaagg ggagtgcagt ctctcctgtc accatcgagg
     8821 acaaggaaac cagtaagcat atgtttcagg ttatgcgctt gaaagaagag gatgaggtta
     8881 ccttagtctt tgatgatggc atcaagcgct tggcgcgcgt gctggatatg gaaaatcgtc
     8941 agtttgagtt ggtccaagaa ttaaatgaca atgtggaact accagttcaa gtgaccatcg
     9001 catctggatt tcccaaggga gacaagctgg agttcatcgc tcaaaaagta accgaactgg
     9061 gtgctagcca aatctgggcc tttcctgcag actggtcagt tgccaagtgg gatggcaaga
     9121 aattgggtaa aaaagttgaa aaactagaaa aaattgccct tggagcagcc gagcaaagca
     9181 agcgtaatat tgtaccaagt attaaacttt tcgagaaaaa agcagatttt ctagctcagc
     9241 tggaccagtt tgactctgtc atagtagcct atgaagaatc aggtaaagaa ggagaagccg
     9301 ctgcgcttct gcaagcagtc actggtcttg aaacaggagc caaactgctc tttatctttg
     9361 gtccagaagg cggtctctca cctgcagaaa tcgaaagttt tgaagctaag ggagccgttt
     9421 tggtaggcct aggtcctcgt attttgcgag cagaaacagc accgctttac gccttatcag
     9481 cccttagtgt tttagtagaa ttagagaaat aagaggaaga aaatggaaca aaaacaccgt
     9541 tcagaatttc cagagaagga actctgggac ttaacagccc tataccaaga ccgtgaggat
     9601 ttcttgcgtg caatcgagaa agctcgcgaa gacatcaacc agtttagccg tgattacaag
     9661 ggcaatcttc acacttttga ggatttcgag aaggcctttg cggaattgga acagatctac
     9721 attcagatga gccatattgg caactatgct tttatgcctc agacgacgga ctatagcaat
     9781 gacgaatttg ccaatattgc ccaagctggg atggaatttg aaacagctgc cagcgtagcc
     9841 ttgaccttct ttgacgatgc cttggtggca gcagacgagg aagtcttgga ccgtttgggt
     9901 aaattgccac atttaacagc tgccattcgt caggctaaaa tcaaaaaagc ccactactta
     9961 ggggcagatg tggagaaggc cttgacaaat ctcggtgaag ttttttacag tccgcaggac
    10021 atttatacta agatgcgagc tggggatttt gaaatggctg actttgaagc ccatggcaag
    10081 acctacaaaa acagctttgt gacctatgag aatttctacc aaaaccatga ggatgctgag
    10141 gttcgtgaga aatccttccg ttccttctca gagggacttc gtaagcacca aaatacggct
    10201 gcagcagcct atctggctca ggtcaagtct gaaaaactct tggctgatat gaagggatac
    10261 gactctgtct ttgactatct tctagctgaa caagaagtgg accgtgtcat gtttgaccgc
    10321 cagattgacc tcatcatgaa ggactttgca ccagtcgctc agagatacct caagcatgtt
    10381 gccaaggtaa atggtcttga aaagatgacc tttgcagact ggaaattgga cttggacagc
    10441 gccctgaatc ctgaagtgac tattgacgat gcctatgatt tggtcatgaa gtcggtagaa
    10501 cctttggggc aagaatattg tcaggaagtt gctcgttacc aagaagagcg ctgggtggac
    10561 tttgctgcta acagtggcaa ggattccggt ggttatgcgg cggacccata tcgcgtacac
    10621 ccttatgtac tcatgagctg gacaggccgt ttgagcgatg tctatacctt gattcatgaa
    10681 atcgggcatt ctggtcaatt catcttttca gacaatcatc aaagctattt caacgcccac
    10741 atgtcgacct attatgttga agcaccgtca accttcaatg aattgctact cagtgattac
    10801 ttagagaacc agtctaatga cccacgtcaa aaacgcttcg ctctggctca tcgcttgaca
    10861 gacacctact tccataattt catcacccac ctcttggaag ccaccttcca gcgtaaggtg
    10921 tatacattga ttgaagaagg ggagaccttt ggagcaagca agctcaacag cattatgaag
    10981 gaagttttga cagatttttg gggcgatgcc attgaaatcg acgacgatgc agctttgact
    11041 tggatgcgcc aagctcacta ctatatgggc ttgtatagtt acacttactc agcaggacta
    11101 gttatctcga ctgctggtta ccttcatcta aaacattctg aaactggagc tgaagactgg
    11161 ctcaatctcc ttaaatcagg tggtagcaag acaccacttg agtcagccat gattatcggt
    11221 gctgatattt caacagacaa accactccgt gataccatcc aattcttgtc tgacacagtt
    11281 gaccagatta tctcctatag tgctgagttg ggagagtagg ggaatagaaa aattttttag
    11341 aatgatgttt atgggcttgt taagcgtcat tctatttgtc ttagttgtag ctattttcaa
    11401 aaatgtcgtt gaaggtcgtg atgttaaatt tgaacttact gtagaactgg tattgatagt
    11461 tatttacgtc attttatatc agattgtatt taattgagaa ttcaatattg agtcatttaa
    11521 gactgccttt ttgaacagtt gctagaaatg tagaggtatt gaccatgtat caagatttac
    11581 taagaaaaat agcagaagaa aaaccaaatt ataaccagga agaaatccag tggttgtttg
    11641 atcatttggg aaatccttct ccagaaattc gtgatgacct ttcaaatcaa ggcttgcact
    11701 atctttcaaa agaaaaggat acaacaggtt tctcaagtca gtatggttgg gttcattctt
    11761 ttgcacatgg agccgattta ctgacagagg tggtttgtca tccagacttt cctaaaaaca
    11821 gagttcatga agtatttgat atacttggcc aactatttaa aagaatgtcg attcgcttta
    11881 cagatgatga ggattggcgt ttagcaagag tgatctatga acctatttta caagggaagt
    11941 tggagcaaga acaagtagct tcttggataa aaactgttga ttttccgata gaagaaaggg
    12001 aggattttta caaattttcc aactttagat cctgtctggt ggaagtctat gtccaacttg
    12061 accagagaaa tagtttacaa gatgacctga aagaagctat ccagtctttt caatactagg
    12121 gattagctaa tagttctatg attttcaaaa aatttccaat caattttctt gaaacccttt
    12181 cctacttttg atagactagt atctagtttt tgaaaaaagg agaaagaaaa tgaaaaaatt
    12241 tgttgctgag ttaatcggta cgttcatgct tgtgttcgtt gggacaggag ctgttgtttt
    12301 tggaaatggt cttgatggcc ttggtcacct tggaatcgcc tttgcctttg gtttggcaat
    12361 cgtggtggcg gcctactcaa tcggaactgt ttcaggtgct cacttgaacc cggctgtttc
    12421 gattgctatg tttgtaaaca aacgtttgtc atcttcagaa cttgtaaact acatccttgg
    12481 tcaggttgtt ggagctttca tcgcttctgg cgcagtcttc ttcctcttgg gtaattcagg
    12541 tatgtcaact gctagtcttg gtgaaaatgc cttggcaaac ggtgtcactg tctttggtgg
    12601 tttcttgttt gaagtcatcg caactttctt gtttgtcttg gttatcatga ctgtgacttc
    12661 agaaagtaag ggcaatggcg cgattgctgg tttggtaatc ggtttgtcat tgatggcgat
    12721 gattcttgtc ggattgaaga ttactggact ttcagtaaac ccagctcgta gcttggcacc
    12781 agctgtcttg gtaggcggcg cagcccttca acaagtttgg attttcatcc ttgcaccaat
    12841 cgctggtgga gttcttgcag cccttgttgc aaaaaatttc cttggaacag aagaattatt
    12901 gaaactcaaa aagccttgct cctcatcttg aggaataggg ctttttcgta tgatactctt
    12961 cgaaaatctc ttcaaaccac gtcagcttca ccttgccgta ggtatggtta ctgacttcgt
    13021 cagtttcatc tacaacctca aaacagtgtt ttaagctgac ttcgtcagtt tcatctgcaa
    13081 cctcaaaaca gtgttttaag ctgacttcgt cagtttcatc t
//
LOCUS       DAVBGY010000053        12573 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVBGY010000053 DAVBGY010000000
VERSION     DAVBGY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12573
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.053"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(251..433)
                     /locus_tag="VQ620_001622"
     CDS             complement(251..433)
                     /locus_tag="VQ620_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764216.1"
                     /translation="MAVIFKNVLIEEKTEEEKQHILSILNGEYDLSDDVSCRLRRLNE
                     ESKRLEVFVKETNSLV"
     gene            complement(649..1881)
                     /locus_tag="VQ620_001623"
     CDS             complement(649..1881)
                     /locus_tag="VQ620_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET2764217.1"
                     /translation="MKIAVAGTGYVGLSIAILLAQHHQVVAVDIIPEKVELINKKKSP
                     IQDDYIEKYLAEKELNLVATLNAEEAYENADFIVIAAPTNYDPKKNFFDTSSVEAVIE
                     AVMKYNPDAIIIIKSTVPVGYTKSVRRKFLTDNIMFSPEFLRESKALYDNLYPSRIII
                     GTDKDDKDLVKSAEIFVKMLQEGAVKENIETLYMDFTEAEAVKLFANTYLALRVSYFN
                     ELDTYSEMKGLDTQAIIDGVCLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYSDVPQ
                     EMITAIVQSNRTRKDYIADRILDLAGSYVGNDFYKPDKEKEIVVGIYRLTMKSNSDNF
                     RQSSIQGIMKRLKAKGVTIIIYEPTLESGTTFYGSQVVNDFEIFKKSSQVIVANRFDE
                     TLEDVRGKVYTRDIFKRD"
     gene            complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQ620_001624"
     CDS             complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQ620_001624"
                     /EC_number="5.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate 4-epimerase Gla"
                     /protein_id="HET2764218.1"
                     /translation="MRGKTILITGVAGFIGSNLVRKLLELEGTMTIIGLDNLNDYYDI
                     ALKDYRLLQLNKLIQDYPEKNWIFIKGDIADKMLINKIFKSYHPQIVVNLAAQAGVRY
                     SITNPDVYIESNIVGFHNVLEACRHSYEIYDGGVEHLVYASSSSVYGGNKKIPFATED
                     KVDNPISLYAATKKTNELQAYAYSKIYNIPSTGLRFFTVYGPAGRPDMAYFGFTDKLR
                     SGSDIEIYNHGNCKRDFTYIDDIVEGIVRVMQSAPKKLVGSDNLPLAPYKVYNIGNSK
                     PENLLDFVDVLQQELIKAGVLPENYDFDSHKKLVPMQPGDVPVTYADTSDLERDFGFK
                     PKTSLREGLRKFAEWYKDYYNV"
     gene            complement(3048..4217)
                     /locus_tag="VQ620_001625"
     CDS             complement(3048..4217)
                     /locus_tag="VQ620_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET2764219.1"
                     /translation="MRLSKKYWFALTSKFLLILLTLLSSIIINRGLGVEKKGDYAYVM
                     NLVEIFYILGGVGLGQAYSTFKRNHSNEYQNKFVFLTFIQGLLFGIIGLIIVLADVPI
                     DYGGVIIVLSSLSIMRTNLTMIAVIENSIKRNKIVLGVSVVYSVMLVLLLLFHLVSIE
                     MVFLVYAINEFLRIILFLYYYNMFPVFEHISIKEFFTIYKFGFLSMILTLLITFNYSI
                     DIIMLKQMSSSYNVGIYSVAVTFSNMFLMVPDSFKEILFGDSAKRTFEKNKAINAIKV
                     SFLFMILLIIGFLSFGEFAITLLYGSSYIKAFPITLVIFLGSLSMIFFKILQPIYISQ
                     GKQSVAIKILSVSALINISLNWYLIPNYQITGAAIASAISYTVCGLFFIIDYLRN"
     gene            complement(4403..5707)
                     /locus_tag="VQ620_001626"
     CDS             complement(4403..5707)
                     /locus_tag="VQ620_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HET2764220.1"
                     /translation="MKFSLQSNDIKNKIILLVGVVFSGTLILCSENNDVTIPLFSMIF
                     LSFMAMIIVKFNIFHPYVWYSSFFTIYSISYPLLYRMEYINYGYTKDLIFMQWLALAT
                     LLLTLPSKAIVLSERKINLNNNNYLLFLNNMNTFITTVAIIYLLGSGFRNKGEIYSGA
                     NIVIMLVFSLVYFMILIHTYQLFIQLSTKKWRLLNCNTNTAIVICLFSIVTGERDYMF
                     TLFILDVIILFYFHKIKRFYLVMLVPLAALLIPLSAVFKYTLLSGQVSSINTNNIWFD
                     LLDGEFVSASRNLQILILHNMGNYFEGRSFFNDIVRIFYNTGYSNQTWFMDTFFPNVH
                     STKYGFTLVGEGYVNGGYFGIVMIFMLTGFLMRFLYINAQRNIYGMLIYLYMIPIFIY
                     STRADFANILSPLLKYAILGTLVIVFINRMVLISPLDEMGGE"
     gene            complement(5824..6978)
                     /locus_tag="VQ620_001627"
     CDS             complement(5824..6978)
                     /locus_tag="VQ620_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2764221.1"
                     /translation="MKVFIATEFRCTIYKNEIYLKPKAYFIYERYAKAFGEIVLCSRY
                     EKVESYPEGYHKANFINKFIPIEGLHQVLFGQNKNKIIEGMIDSDLIVVRIPSIIGSK
                     TADYALKIGKPYLTEIMGDAWDSYWYHSLKGKLLAPYIYAKTKSIVKNANYCIYVTEK
                     YLQDRYPNIKSNIVASNVNITSVENRSLKSRLYKLKKFNPQKISIMTTASVNVRAKGH
                     RFVLEAMKRLEIQGILLDYYLAGDGDQSFLKKKAEELGVANRIHFLGELTTSQIYEYL
                     DKVDLYIQPSLQEGLPRAVIEAMSRACPCIGSNKAGIPELLGPHCLFTPSSSQAIAGS
                     IIDFISMDKEKMVIKQFEFSKKFLSSVLNKRRSDYFAQIRRELNNSDGLD"
     gene            complement(6975..7526)
                     /locus_tag="VQ620_001628"
     CDS             complement(6975..7526)
                     /locus_tag="VQ620_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET2764222.1"
                     /translation="MNRIIKNIVGLLRANICLILLKVCRNKDLTFHYFLKFGKNVTVE
                     QESNAKLILGKKIRVNAGGVLKVRKGAKLKISDDVFLSNNCMIACRKYIDIKSGVKCG
                     PGVLIYDHDYDVSVPGGLKAKKFKTAPVMIGENVWIGANSIVLKGVSIGENSVVAAGS
                     VVTKDIPADTIFIQKRLSREMKL"
     gene            complement(7519..8655)
                     /locus_tag="VQ620_001629"
     CDS             complement(7519..8655)
                     /locus_tag="VQ620_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2764223.1"
                     /translation="MNLQKKKIMLITNHDDDIYCFRKEVIESLVEYGYEVLVSCPYGD
                     KIELMKSIQFEYEDITIDRRGINPLKDIRLFLSYIRLLRKYRPDVVLTYTVKPNVYAS
                     LAATILGIKYINNVTGLGSVLTMGSCVRSLVLLLFRISFRKSNCVFFQNEYNMKMLKT
                     LGLILGDSKLIPGSGVNIEKYPVQQYPNGGNGIQGETIVFNFIGRILKEKGIDTYLAA
                     AQIIKSRYPKTEFNIIGFIEPTESNYELKICDLEKKGIVYYLGQQKDVIPHITRSHAI
                     IHPSVYGEGMSNVLLENASSGRVLITTDNPGCKEIVKDRETGYIFQGGNVEELVSILE
                     VFLGLENEKRKEMGLQGRMYIEQYFSRQIIIDAYLDKIKGALDE"
     gene            complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQ620_001630"
     CDS             complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQ620_001630"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HET2764224.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNTLCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIAMSFARAGYKTLLFDGDIRNSVMSGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTPPIGIVI
                     DAAIITQKCDASILITATGEANKRDVQKAKQQLEQTGELFLGVVLNKLDISVDKYGVY
                     GRK"
     gene            complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQ620_001631"
     CDS             complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQ620_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HET2764225.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQGEKPGLTNQDLQAGAYLVKDYREIVLSQDVLEEVISDLKLD
                     LMPKGLANKIKVTVPVDTRIVSVSVSDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNTLIGFLAGVIGTSVIVLLLELLDTRVKRPEDIENALQMT
                     LLGVVPNLGKLK"
     gene            complement(10172..10903)
                     /locus_tag="VQ620_001632"
     CDS             complement(10172..10903)
                     /locus_tag="VQ620_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase CpsB"
                     /protein_id="HET2764226.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKELFVDNPRKIIMDQLI"
     gene            complement(10905..12350)
                     /locus_tag="VQ620_001633"
     CDS             complement(10905..12350)
                     /locus_tag="VQ620_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET2764227.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLICFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLLFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSKIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADS
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLTDGDRDRGRNQQKVIVAIIQKLTST
                     EVLKNYSSILQGLQDSLQTNIPIETMIDLVNTQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLTVVKAAIQDVMEGR"
BASE COUNT         4238 a         2434 c         1654 g         4247 t
ORIGIN      
        1 aattcactta cttcatatta catttagcat gaaatgaaat aagcgaattc tactttgttt
       61 gcatacaatt aaaaatcaat tcctaaggat gttttaaata actgtgtaaa aggcataatt
      121 tcaccatttc ttagtttttc aatgctaaat tacatagtaa ctggagagct attagaaatc
      181 tctaacttcc tcatagttaa aaaatgacaa tgagatactt attgatttca attcattaac
      241 agaatacgaa ctagacaagt gagttcgtct ccttaacaaa cacttccaag cgtttacttt
      301 cctcattcag tcgtcgtaat ctacaactta catcatcaga taagtcatac tcaccattta
      361 gaatagatag aatatgttgt ttttcttctt cagtcttctc ttcaataagc acatttttaa
      421 aaattactgc cattatcagc acctcacttc gaaggaatat atgcaactat atcctattaa
      481 gtcaatctct actgacaaaa gaagtatatt tacttcacta agtgcgagcg agaatataga
      541 aatatcttta ttgttttaga tgagttgtcg ctatcctcca gctagttttt aactattcta
      601 tcattcaaat gatatagatt attagggact agaaatatac aaattgattt aatctctctt
      661 aaaaatatcc ctagtataaa ctttacccct cacatcttct aaagtctcat caaaacgatt
      721 tgcaacaatc acttgactag atttcttaaa tatttcaaaa tcattaacta cttgactacc
      781 atagaaagta gtaccacttt caagagttgg ttcgtaaata ataatagtaa caccttttgc
      841 tttcaaacgc ttcataatac cttgaataga agattgtcta aaattatctg aattactttt
      901 cattgttaaa cgataaattc ctacaacaat ctctttttct ttatctggtt tatagaaatc
      961 attgcctaca taagatccag caagatctaa aatacgatct gcaatataat cttttcttgt
     1021 ccgattactt tgtactatag ctgttatcat ttcttgtgga acgtcagaat aatttgcaag
     1081 aagttgcttg gtatctttag gaagacagta acccccataa ccaaaagaag gattattgta
     1141 gtgagaacct attctcggat ctaaacaaac tccatcaata atcgcttgag tatctagtcc
     1201 tttcatctca gaataagtat ctaactcgtt aaaataggaa acccggaggg caagataggt
     1261 attagcaaaa agttttactg cttcagcttc tgtaaaatcc atatacaatg tttcaatatt
     1321 ttctttcact gcaccttcct gaagcatttt tacaaaaatt tcagcagact ttaccaaatc
     1381 tttatcatct ttatctgtac ctataataat tcgactcgga tataggttat catataacgc
     1441 ttttgactcc cgtaaaaact caggactaaa cataatattg tctgtaagaa atttcctccg
     1501 tactgacttt gtatatccaa caggaacagt acttttaatt attataattg catcaggatt
     1561 atatttcata actgcttcaa tgacagcctc gactgaagat gtatcgaaaa aatttttttt
     1621 cggatcataa ttagttggag ccgcaataac tataaaatcc gcattctcat atgcttcctc
     1681 tgcattcaat gtagctacga gattcaattc tttttccgcg agatattttt ctatataatc
     1741 gtcttggata ggggacttct ttttattgat taactcaact ttctcaggaa tgatatctac
     1801 ggcaacaact tgatgatgtt gagccagtaa gattgcaata gataatccaa catatcctgt
     1861 acctgctact gctattttca taattttttc ctttctcaaa acactctcgt tatacattat
     1921 agtagtcttt ataccactct gcaaactttc tcaaaccttc tcgtaaagaa gtttttggtt
     1981 taaaaccaaa atctctctct aaatcacttg tatctgcata agtaacagga acatcgcctg
     2041 gttgcatagg aactaatttt ttatgactat caaaatcata attttccggc aaaactcctg
     2101 ccttgattag ctcctgttga agaacatcca caaaatctaa caaattttcg ggtttactat
     2161 tcccaatatt gtacacttta tatggggcta acggtaaatt atcagatccc actagtttct
     2221 tcggagctga ttgcatgacc cttactatcc cctcaacgat atcatctata taagtaaaat
     2281 ctcgtttaca attaccatga ttataaatct caatatcgga accacttcgc aatttatctg
     2341 taaatccaaa ataggccata tctgggcgcc cagcaggacc ataaactgta aaaaagcgta
     2401 agcctgtcga aggaatattg taaatctttg aataagcata cgcttgaagt tcatttgttt
     2461 ttttagtcgc agcatatagt gaaataggat tgtctacctt atcttctgtt gcaaacggga
     2521 tttttttatt tccaccatat accgatgagg acgatgcata aactaagtgt tccacaccac
     2581 catcatatat ctcatacgaa tgacgacaag cttccaacac attatgaaag ccaacgatat
     2641 tactttctat atatacatct ggatttgtaa tggagtagcg tactcctgcc tgggcagcta
     2701 gattaacaac aatctgagga tgataactct taaaaatctt attaataagc attttatctg
     2761 caatatcacc cttgataaat atccagtttt tttctggata atcctgaatt agcttattaa
     2821 gttgaagcaa tcgataatct ttaagtgcaa tatcatagta atcattcaaa ttgtccaaac
     2881 caatgattgt catcgttcct tctaattcca ataatttcct aaccagattt gatccaataa
     2941 aaccagctac tcctgtaatt agaattgttt ttcctctcat atcataatac ctcttgttat
     3001 ttttatattt tcatccacta atacacattc tttaacaact attataatca atttctaaga
     3061 taatctataa taaaaaacaa accacataca gtataagaga ttgctgatgc tatcgcagct
     3121 cctgtaattt ggtagttagg aattaagtac cagtttaaac ttatatttat caaagccgac
     3181 acagataaaa tttttatagc tacactctgt ttaccctgag atatataaat tggttgtaaa
     3241 attttaaaga aaatcatcga taagcttcct aaaaaaatga ctaatgttat aggaaatgcc
     3301 tttatataac tggaaccata aaggagcgta atcgcaaatt ctccaaaaga aagaaatcca
     3361 ataattaata aaatcataaa tagaaatgat acttttattg catttattgc tttatttttc
     3421 tcaaaagttc ttttagccga atcaccaaat aaaatctctt taaatgagtc tggaaccatc
     3481 aaaaacatat tagaaaacgt aactgcgaca ctgtatatac ctacattata actactagac
     3541 atttgcttca acattatgat atcaatacta taattaaatg taattaatag agtaagaatc
     3601 atagaaagaa atccaaactt atatatagtg aaaaattctt taatactaat atgttcaaaa
     3661 actgggaaca tattgtaata gtataaaaat aaaatgatac gtaaaaattc attaatagca
     3721 tataccaaaa ataccatctc tatagaaact aaatgaaata gtaacaacaa tactagcata
     3781 acggaatata ctacgcttac tcctaataca atcttattcc tctttatact attttctatt
     3841 actgcaatca tagtaagatt tgttctcata atagatagac tggacaaaac tattattacc
     3901 ccaccataat ctatcggaac atcagctaaa actattatta aacctattat tccaaacaat
     3961 aatccttgaa taaaagttaa aaaaacaaat ttattctgat actcattaga atgattcctt
     4021 ttaaaggtcg agtaagcctg acctaaacca actccaccta aaatataaaa aatttctact
     4081 aagttcatta cataggcata atctcctttt ttttcaacac ctaatcccct attaattata
     4141 attgaggata aaagtgttaa taagattaac aaaaatttac tcgttaaagc aaaccaatat
     4201 ttcttagata gtctcactta ttttctccgt atgttaaatt ttataacagt aaatttaaaa
     4261 ttatagtata ttgaaataga atatgaacaa aacgattagg gaatttaaag taatttataa
     4321 aaattcttta gaagctgcat tgtactactc caacttcaaa ccactatagt atcaaaaagt
     4381 ataccccacc tttttaaata aactactcac cacccatttc atccaatggt gatatcaaca
     4441 ccattctatt gataaaaaca ataaccaatg ttcccaatat agcatattta agtaatggag
     4501 ataatatgtt agcaaaatca gcacgagtag aataaataaa aataggtatc atatacagat
     4561 atatcagcat cccgtatata tttctttgag cgtttatata aagaaatctc atcaaaaagc
     4621 ctgttagcat aaaaatcatt acaattccaa agtatcctcc attcacatat ccctctccca
     4681 caagagtaaa accatatttg gttgaatgta cgtttggaaa aaaggtgtcc ataaaccaag
     4741 tttgattaga ataccccgta ttataaaaaa ttcttactat gtcattaaaa aaagaacgac
     4801 cttcgaaata attgcccata ttatgtaata tcagaatctg taaattacga ctagctgata
     4861 caaactcacc atctagcaaa tcaaaccaaa tattatttgt atttatagat gaaacttgcc
     4921 cacttaataa agtatattta aaaactgcag ataacggaat cagtagtgcc gcaagcggaa
     4981 ctaacattac aagataaaaa cgttttattt tgtgaaagta aaataatata atcacatcaa
     5041 ggatgaataa agtaaacata taatctcttt cccctgtaac gatagagaag agacagataa
     5101 ctatcgctgt gttagtatta caatttagta gtctccattt tttagtactc aactgtataa
     5161 atagctgata tgtatgaatg agaatcataa aataaaccaa agaaaatact aacattatca
     5221 caatattggc acctgaatat atttcccctt tatttctaaa accacttcct aatagatata
     5281 taattgctac tgttgtgata aacgtattca tattattgag aaataataaa tagttattgt
     5341 tatttaggtt tattttcctt tcgcttaaca caatggcttt agaaggtaga gttaacaaca
     5401 aagttgccaa agccagccat tgcatgaaaa tcaaatcttt tgtataacca tagtttatat
     5461 actccattct atagagtagc gggtaagaaa tagaataaat agtaaagaag ctactatacc
     5521 acacataggg atgaaaaata ttaaatttca caattatcat tgccataaaa cttaaaaaaa
     5581 tcattgaaaa taatggaatt gtaacatcat tattttcact gcataaaatt aaagtccctg
     5641 aaaaaactac acctactaga agtattattt tattttttat atcatttgat tgtagactaa
     5701 acttcattta cttttttcct ttaactaata atttaaacgt aatgcgtatt caaaaatttg
     5761 attggctatg tagtcagttt ttatagcata tttaatatat cgaaacggat tttccatcgc
     5821 attttaatct aaaccatcag aattatttaa ttctcttcta atttgagcaa aatagtcact
     5881 tctgcgttta tttaatacac tcgaaagaaa tttttttgag aattcaaact gttttatcac
     5941 catcttttcc ttatccatac taataaaatc tataatagat cccgcaatcg cttgtgaaga
     6001 ggatggagta aatagacaat gtggccccag caactctgga attcctgctt tattagaccc
     6061 tatacaaggg caagcacggc tcatagcttc aattaccgca cgaggtaacc cttcttgtaa
     6121 acttggttgt atatataaat caactttatc caaatactca taaatttgtg atgtagtcaa
     6181 ttcccctaaa aaatggattc tattcgctac tcccaattcc tctgctttct tttttaagaa
     6241 actttgatca ccatcacctg ctaaataata atccaacaaa ataccttgta tttctaatct
     6301 cttcattgct tccaatacaa atctatggcc cttggctcgt acattcacag atgctgttgt
     6361 cattattgaa attttttgag gattaaattt tttcaactta taaagacggc tcttcaaaga
     6421 tctattctct acagaggtaa tattaacatt tgaagcaacg atattagatt taatattagg
     6481 atatctatct tgtaaatatt tttctgtcac gtatatgcaa taattagcgt tttttacaat
     6541 tgatttagtt ttggcgtata tatatggagc taataatttt ccctttaaac tatgatacca
     6601 gtaagaatcc caagcatccc ccattatttc tgtcagatac ggcttaccta tcttcaatgc
     6661 gtagtctgca gtttttgatc caattataga cggaatacga acaactatta agtcgctatc
     6721 aatcattcct tcaataatct tatttttatt ttgaccaaat agaacctgat gtagaccttc
     6781 tatagggata aatttattaa taaagttcgc tttatgatat ccttcagggt atgattccac
     6841 cttctcataa cgactacata gtacaatctc tccaaatgct ttagcatagc gctcataaat
     6901 aaaataagct ttcggtttta aatatatttc atttttatag attgtacatc taaattcagt
     6961 cgctataaat actttcataa tttcatctcc cttgataaac gtttctgaat aaatatagta
     7021 tcagctggaa tatcctttgt tacaacactt cctgctgcaa ccacactatt ctcaccaata
     7081 ctcactccct tcaagacaat gctgttagct ccaatccaaa cattttctcc aatcataact
     7141 ggggccgtct taaatttttt tgctttcaat ccacctggaa cactaacatc atagtcatga
     7201 tcatatataa gtactccagg accacatttt actccagatt taatatctat gtatttacga
     7261 caagctatca tacaattatt actcaaaaat acatcatcag aaatcttgag ttttgctcct
     7321 tttctaactt tcaatactcc cccggcgttt actctaatct tttttcctaa gattaattta
     7381 gcattagatt cctgttcgac agttacattc tttccaaatt ttaaaaaata atgaaaagta
     7441 agatccttgt ttcgacacac ttttaataat atcaagcaaa tatttgcacg aagtaaacca
     7501 acaatatttt ttataatcct attcatctaa agctcctttt attttatcta aataagcatc
     7561 tataataatt tgacgagaaa aatattgctc tatatacatt ctcccttgaa gtcccatctc
     7621 ttttcgtttt tcattttcta gacctaaaaa aacttccaat atagagacta gttcctcaac
     7681 atttccccct tgaaatatat agcctgtctc tctatcttta acaatttctt tgcaacctgg
     7741 attatctgtc gtaattaaaa cacgtcctga actagcgttt tctagtaata cattgctcat
     7801 tccttcacca tacacactgg gatggataat tgcatgggaa cgggtaatat gaggtatcac
     7861 atctttttgt tgtcccaaat aataaacgat tccttttttt tctaagtcac aaattttaag
     7921 ttcataatta ctctctgtcg gttctataaa gccaataata ttaaactctg ttttgggata
     7981 tcgactctta ataatttggg cagcagccag ataagtatct ataccttttt cttttaatat
     8041 cctaccaata aaattaaaaa cgattgtttc tccttgaatt ccatttccac catttggata
     8101 ctgttgcaca ggatactttt caatgttcac tcctgaccca ggtatcagct tagagtcacc
     8161 caaaatcaag ccaagagttt ttaacatttt catattatat tcattttgaa aaaaaacaca
     8221 atttgatttc ctaaaagaaa ttctaaacag taataacacc aggcttctta cacaagaacc
     8281 cattgttaag acactaccta aacctgttac gttatttata tattttattc ctaaaattgt
     8341 tgcagctagg ctagcatata catttggttt cacggtatat gtcaagacta catccggtct
     8401 atattttctc aataatctta tataactaag aaatagacga atatctttta aagggtttat
     8461 tcctcttcta tcaatagtaa tatcttcata ctcaaattga atagacttca tcaactctat
     8521 cttatctcca taaggacatg acactagcac ttcataccca tactcaacta gtgactctat
     8581 aacttctttt cgaaaacaat agatatcatc gtcatgatta gttattaaca taattttttt
     8641 cttttgtaaa ttcatcaaaa taatcttcct aaaatataat taaccgctac tccattatcc
     8701 tttacaacta tcattttaaa aatagtaatt ttctataagt aattgtttcc aaattgtgac
     8761 actttaaaat tctatacaca tatagaactt acaaactatt tacgaccgta aactccatac
     8821 ttatcaaccg agatatccaa tttatttaaa acaactccca ggaacagttc ccctgtttgt
     8881 tctaattgtt gtttcgcttt ttggacatca cgtttattcg cctcacctgt tgctgttatc
     8941 aagatggacg catcacactt ttgagtgata attgccgcat caataacaat tccaataggt
     9001 ggtgtatcaa caatgatata atcaaaatat ttacgcaatg tttcaatcat atcattaaaa
     9061 tttttacttt gtaacaaggc tgtagggttt ggtgatacag atcccgattg aactacaaat
     9121 aaattttcaa tatttgtatc acataaaccg tgagataaat cagctgtccc agataaaaat
     9181 tctgttagcc ctgtaatttt ttcacgagat ttaaaaaatc ctgacataac tgaatttcga
     9241 atatcgccat cgaacaaaag agttttatag cctgcacgcg caaatgacat tgctatattt
     9301 acggaagtag ttgtttttcc ttcccccgga ttaacagaag taacggaaat tacttttagt
     9361 ttatctccgc tcaactgtat atttgtacac aaggtattgt aatattcttc tgccttctta
     9421 atgaactcca gttttttttg tgctatttct aatgtcggca tctctctctc ctatttcaac
     9481 ttacccaagt ttggtacaac tcccaaaagt gtcatctgca gtgcattttc gatatcttcc
     9541 ggacgtttca cacgagtgtc caaaagttca agaagaagaa ctataacact agttccaatc
     9601 acccctgcca aaaaaccaat tagtgtattg cgtttaatat ttggcgaaga cggtgatatc
     9661 gccggtcttg cctcctccag tgttgtcaca tcagaaacac gagtaatact gataattttt
     9721 tgagcagcta cttctcttaa agagttagcg atacggcttg cctcttcagg aactcgatca
     9781 ctaactgaaa cagagacaat acgggtatca actggtactg ttactttaat tttattagcc
     9841 aaaccttttg gcatcaaatc tagtttcaaa tcagaaataa cttcctccaa aacatcctgc
     9901 gataggacaa tttcacgata gtctttaacc aagtatgctc ctgcctgcaa gtcttgattg
     9961 gttaaaccag gcttctctcc ctgattacgg ttaactacat aaatccgagt cgtactagta
    10021 aactcaggtt tgataacaaa agtactgtag gcaaaagcaa ctgaagaagt tataattgcc
    10081 actaataaaa tgaccaactt tcttttccat aaagctctga atagttgcaa tacatcgatt
    10141 tccaaagtgt tttgttcctt catattttct cctaaattaa ttgatccatt ataatttttc
    10201 tgggattatc tacaaaaagt tctttcgctt ttttcgctcc atatttctta gcaatgatat
    10261 catatgcctg ttgcatatat ggaggtctac tgtctaaatt gtgcatgtca cttgcaacta
    10321 catgaactaa atcacgttcc aaaaaatacc gagctctctt tttcatgaat ttatattttt
    10381 caccaaagaa cttaggtttt gaaacatgat aactatttat ctgagtatag caccccatat
    10441 caatcagttc acgaacacgt ttttcgttat tctctaaagc atcataacgt tcaatatgag
    10501 caattactgg cgtgattccc aacatcaaaa tattgctcaa tcccgtatga atctgacgat
    10561 aggaagtatg catgctaaac tcaatcaagg cataacgact atcattaagg gtaggaattt
    10621 cttttttttc tagcttttct agagcatcca gagtatagta tatctctgcg ccataagcaa
    10681 tgactaaatc atctgctact tcttttgcaa tttcccgaac ctgaagaaag ttttctgcta
    10741 tcttctcttc cggagtttca aacatgccct tgcgacggtg agaggtagag acaatggttc
    10801 tcaccccctg cctgtaggat tctgccaaga gagccttgct ttcctctctt gacttgggac
    10861 catcatctac atcaaagacg atgtgcgaat ggatgtctat cattttatct accctccatc
    10921 acatcctgta tagctgcttt aactacagtt aaactactat catctatttc catcacatag
    10981 aggttactgt ctggcattgc ataagaagga agatccatcc gacctgtccc ttttaaatct
    11041 tgagaattta ctttataatt ccctccactt tccaactgag tattcactaa atctatcata
    11101 gtctcaatcg gtatatttgt ttgaagagaa tcctgcaatc cttgaagaat actactatag
    11161 tttttcaaaa cctctgtaga agttaacttc tgaataattg ctacaatgac cttttgttgg
    11221 ttgcgaccac ggtcacggtc tccatcggtt agtgagtagc gttcacgtac aaaacctaaa
    11281 gcttgctcag agtctagatg gacattccct actgggaaat ggaacttccc atgtagagct
    11341 gaaaactctt gatcattatg aacatctacc cctcccaata agtcaatcat tttcaagaaa
    11401 gaagtgaagt tcaatcgcac atagtaattg atatccactc catagagatt ttctaaggtg
    11461 tgaatggacg aatcaactcc ataaatgccc gcatgggtta atttatcttt ttgattattt
    11521 ccactatctg cgattggtac ataggcatca cgtggcgttg tggtcaagag gattttcttg
    11581 gtatctcgat tgacagtcat taggatattg acatctgatc gcgacaccga actaatagga
    11641 ccataggtgt caattccact aacatagata ttgaaagact gattcttaga cgtcttagga
    11701 gcctctactt ttttagtgaa tcccttggta taaatctttt ttatcttcga tgcgtagtct
    11761 ggatactctg actcgatgat attttcaaag acactattta ggacaatggc cttagtctcc
    11821 cctgcaatca aactcttgta agctgccaag taagacgaac tctggttgac cgtcaaatcg
    11881 gtattctgac ttgacttgat atcagctagt agtttttgaa tattttcatt atcagtccca
    11941 gtcggtgctg tcacactcgt cagttgcgta acattttcga tcttactatc tgctaaaaca
    12001 gcgacactga ttgaatattc tgagtaatta gaagtcgcat ttaaacgatt ggtcagtcca
    12061 acaaactgct gtactgcaaa gagcgacact gagctgacaa ggatagagaa cagcaacaga
    12121 aaaatagtaa acttttcagc ttttttatag ataatcaaga gtagccctac caaggcaact
    12181 agtaggacta acgcagttac cactaggtta agatatctaa aagcaaggat attgtactta
    12241 aagattaaga acaataaaaa acaaattaac aataaataaa tagtcagcaa aacgatatta
    12301 acacttcgct tcactttctg tgaacgtgat tttttaaaac gtctactcat gattaacacc
    12361 tataccttga acatcataca attatatcac ttttttacgg taatgtctac acctttattt
    12421 tcactatctg catctttaag tatcttagta gacttcccgc gaaccaaaaa tatagtaaaa
    12481 tgaaataaga ataggacaaa tcaatcagga cagtcaaatc gatttctaac aatgttttag
    12541 aatcagatgt gtactattct agtttcaata taa
//
LOCUS       DAVBGY010000054        11835 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVBGY010000054 DAVBGY010000000
VERSION     DAVBGY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11835
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.054"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            173..2464
                     /gene="pcrA"
                     /locus_tag="VQ620_001634"
     CDS             173..2464
                     /gene="pcrA"
                     /locus_tag="VQ620_001634"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HET2764228.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTTYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2507..3187
                     /gene="radC"
                     /locus_tag="VQ620_001635"
     CDS             2507..3187
                     /gene="radC"
                     /locus_tag="VQ620_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET2764229.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3184..3873)
                     /locus_tag="VQ620_001636"
     CDS             complement(3184..3873)
                     /locus_tag="VQ620_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET2764230.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYNLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(3891..4532)
                     /locus_tag="VQ620_001637"
     CDS             complement(3891..4532)
                     /locus_tag="VQ620_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET2764231.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(4615..4830)
                     /locus_tag="VQ620_001638"
     CDS             complement(4615..4830)
                     /locus_tag="VQ620_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET2764232.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(4838..5185)
                     /locus_tag="VQ620_001639"
     CDS             complement(4838..5185)
                     /locus_tag="VQ620_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HET2764233.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5190..6305)
                     /locus_tag="VQ620_001640"
     CDS             complement(5190..6305)
                     /locus_tag="VQ620_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2764234.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6315..7274)
                     /locus_tag="VQ620_001641"
     CDS             complement(6315..7274)
                     /locus_tag="VQ620_001641"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET2764235.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7387..7956)
                     /locus_tag="VQ620_001642"
     CDS             complement(7387..7956)
                     /locus_tag="VQ620_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HET2764236.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAEDYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8084..8755
                     /locus_tag="VQ620_001643"
     CDS             8084..8755
                     /locus_tag="VQ620_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HET2764237.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            8739..9557
                     /locus_tag="VQ620_001644"
     CDS             8739..9557
                     /locus_tag="VQ620_001644"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HET2764238.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            9554..10450
                     /locus_tag="VQ620_001645"
     CDS             9554..10450
                     /locus_tag="VQ620_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2764239.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            10494..11468
                     /gene="pta"
                     /locus_tag="VQ620_001646"
     CDS             10494..11468
                     /gene="pta"
                     /locus_tag="VQ620_001646"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HET2764240.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT         3441 a         2268 c         2549 g         3577 t
ORIGIN      
        1 aggtattgag ctagtttatg aaaaaattgg gtgaaaaagt cgagtgtttt agaaacccac
       61 agtgtagtat tctagtttca atccactata ttttgctact ccccgtaaag tttctatttt
      121 ccctgatttc tgatataata gaaatattga cttcaagagt aaggaagaga agatgaacgc
      181 attattaaat ggaatgaatg accgtcaggc tgaggcggtg caaacgacag aaggtccctt
      241 gctaatcatg gcaggagctg gttctggaaa gactcgtgtt ttaacccacc gtatcgctta
      301 tttgattgat gaaaagctgg tcaatccttg gaatatcttg gccattacct ttaccaacaa
      361 ggctgcgcgt gagatgaaag agcgtgctta tagcctcaat ccagctactc aggactgtct
      421 gattgcgacc ttccactcca tgtgtgtgcg tattttgcgt cgcgatgcgg accatattgg
      481 ctacaatcgt aattttacaa ttgtggatcc tggtgaacag cgaacgctca tgaaacgtat
      541 tctcaaacag ttgaacttgg atcctaaaaa atggaatgaa cgaactattt tggggaccat
      601 ttccaatgct aagaatgatt tgattgatga tgttgcttat gctgctcaag ctggcgatat
      661 gtatacgcaa attgtggccc agtgttatac aacctatcaa aaagaacttc gtcagtctga
      721 atccgttgac tttgatgatt tgattatgct gaccttgcgt ctctttgatc aaaatcctga
      781 tgttttgacc tactaccagc aaaaattcca atacatccac gttgatgagt accaagatac
      841 caaccacgct cagtaccaat tggtcaaact cttggcttcc cgttttaaaa atatctgtgt
      901 ggttggggat gcggaccagt ctatctacgg ttggcgtggt gctgatatgc agaatatctt
      961 ggactttgaa aaggattacc ccaaagctaa ggttgttttg ttggaggaaa attaccgctc
     1021 aaccaaaacc attctccaag cggccaacga ggttattaaa aataataaaa atcgccgtcc
     1081 taaaaatctc tggactcaaa acgctgatgg ggagcaaatc gtttactatc gtgctgatga
     1141 tgagctggat gaggctgtat ttgtagccag aaccatcgat gaacttagtc gcagtcaaaa
     1201 cttccttcat aaggattttg cagttctcta tcggactaat gcccagtccc gtacaattga
     1261 ggaagccctg ctcaagtcta acattcctta taccatggtt ggcggaacca aattctacag
     1321 ccgtaaggaa attcgcgata ttattgctta tctcaacctt attgctaatt tgagtgacaa
     1381 cattagtttt gagcgtatta tcaacgagcc taaacgtgga attggtctag gtacagttga
     1441 gaaaatccgt gattttgcaa atttgcaaaa tatgtctatg ctggatgctt ctgctaatat
     1501 tatgttgtct ggtatcaagg gtaaggcagc ccaatctatc tgggattttg ccaatatgat
     1561 gcttgatttg cgggagcagc tagaccactt aagcattaca gagttggttg agtccgtcct
     1621 agaaaaaaca ggttatgtcg atattcttaa cgcccaagcg actctagaaa gcaaggcaag
     1681 ggttgaaaat atcgaagagt ttctttctgt tacgaagaac tttgatgaca ccactgatgt
     1741 gacagaagag gaaactggtc tggacaaact gagtcgtttc ttaaatgact tggctttgat
     1801 tgccgacaca gattcaggta gtcaggagac atcagaagtg accttgatga ccctgcatgc
     1861 tgccaaaggt ctcgaatttc cagttgtctt tttgattggg atggaagaaa atgtctttcc
     1921 acttagtcgt gcgactgaag atccagatga attagaagaa gagcgccgtc tagcctatgt
     1981 aggtatcacg cgtgcagaga aaattctcta tctgaccaat gccaactcac gcttgctttt
     2041 tggtcgtacc aattataacc gtccgactcg ttttattaac gaaatcagtt cagacttgct
     2101 tgagtatcaa ggtctggctc gtcctgcaaa tacaagcttt aaggcatcat atagcagtgg
     2161 tagtatttcc tttggtcaag gtatgagttt ggctcaggct cttcaagacc gtaaacgcgg
     2221 tgctgcccca aaatcgatcc agtcaagcgg tcttccattt ggtcaattta cagctggcgc
     2281 aaaatcagca tctagcgagg caaattggtc cattggtgac attgctctcc acaagaaatg
     2341 gggagaggga actgttctgg aagtttcagg tagcggtgct aggcaggaat tgaaaatcaa
     2401 tttcccagaa gtaggtttga aaaaactttt agccagtgtg gctccaattg agaaaaaaat
     2461 ctaattttcc atccttctca cgaataataa agtgaggagg atttttatgt acagtatttc
     2521 attccaagaa gattcactat taccaagaga aaggctggcc aaggaaggag ttgaagcgct
     2581 tagtaaccaa gagttgctag ctattttact caggacagga acacgtcaag ctagcgtttt
     2641 tgaaattgcc caaaaagtct tgaacaatct ttcaagccta acggatttga aaaaaatgac
     2701 cctgcaggaa ttgcagagtt tgtctggtat tgggcgtgtt aaggccatag aattacaagc
     2761 tatgattgaa ctggggcatc gtattcacaa acacgagact cttgaaatgg aaagtattct
     2821 cagcagtcaa aagttggcca agaagatgca gcaggaatta ggggataaaa aacaagagca
     2881 cctggtggca ctctatctca atactcaaaa tcaaatcatc catcagcaga ccatttttat
     2941 cggttctgta actcgtagta tcgctgaacc gcgagagatt cttcactatg caatcaagca
     3001 tatggcgact tctcttatct tggtccacaa tcatccttca ggagcggtag cgcctagcca
     3061 aaatgatgat catgtcacta aacttgttaa agaagcctgc gaattgatgg ggattgttct
     3121 cttggaccat ttgattgtct ctcattctaa ttactttagt tatcgtgaaa agacagattt
     3181 aatctaaagt tcattaacga catagtcaaa gagtttttta tctttgggac gattttcaaa
     3241 aagaagttct ggatgccatt ggacaccgag aaaggcgaca tcatccgtac tcatgacagc
     3301 ctcaatgata ccatctttag gatcatgagc cacaactttt aaatttggtg ctaagtcctt
     3361 gatgctctgg tggtggaagg agttgatatg agagatttct ccatagattt cttggagaac
     3421 ggtatctggt tctgttacca agcgttgagt tgtgtactca acagaagaat cctgccaatg
     3481 gtcttcgata tcttggtaca aagttccacc catggcaacg ttaaagagtt gggtaccacg
     3541 gcagacagag aaaatgggct ttttctgttt aatagcttcc ttgatgaggg ccagttcgaa
     3601 gatatctctt tgaaggttat agtcatcgct atcaatggtt ttgggttcgc cataaaattt
     3661 tggatcgaca ttttgcccac ctgtcaagat gagcttgtca atcaaactga tatagtggca
     3721 ggccatttct tgatcaccaa tcggtaggat gatgggaatc cctccagcat ctttaacgcc
     3781 ttcaacaaag ccttttgctg cgtagctcat catgatgtca tcatctggat gagctttttc
     3841 gtttcctgta atcccaataa ctggtttttt cataaaatga ttttcgcttt ctaatcctct
     3901 tttcgcatga agtagaggag ggtttggagt tcacttgtca aatcgacata ctgaacgacc
     3961 acgtcttttg gtaaatgcag atggactggt gaaaaactga gaattccttt cacaccagca
     4021 tcaaccaaga gattagcaac ctcttgtgac ttgacgctgg gaacagttag gatagcagtc
     4081 ttcacatcag catccttgat tttatccttg atctgagaaa tcccgtaaat gggaatcccg
     4141 tcaggagttt gggtaccgac ttcaggatgg tcgtctaggt caaaggccat gataatcttc
     4201 atcttgttac gttcgtggaa gcggtagtgg agaagggcat ggcccatatt tccaatacca
     4261 accagcatga cattggtaat agagttgtca ttgagcaaat cggcaaaaaa tgtcattagt
     4321 tttttgacat catagccaaa accacgacga ccaagttcac caaaatagga aaaatcacga
     4381 cgtacggtcg ctgaatcaat accgatagcc tctgcaattt gcttagagtt ggcacgttca
     4441 atcttttctg catgaaatct cttaaaaatt cgatagtaga gagagagtct ttttgctgta
     4501 gcttttggaa tagcaaactg tttatctttc acaaaatcac aacctttcta ttcttctatt
     4561 ttatagaaac attgtgaaaa aatcaacaaa aataagaaaa aactaagaaa aatcttagtt
     4621 ttgatgtaaa aaatctgcat gagatagaaa acggtagagg tctccgacca gaccctgata
     4681 aactttttgg cccctaaaag tcagagaagt cacataaagt gtatctggta aggttacaca
     4741 tcctgacaaa gtcaacatga gagcctcatg atcctcatac ttgagagtac gctctacatg
     4801 atagcagtcc ttataggtca gttcaaacat tttggctcta tctttccgat tttgtaaaga
     4861 caccacgttc taccaagcta tccatgagga agtagaattt ttcctgatga atatggtggt
     4921 cttctgattt gaaaatatca actagacgaa ggccaaactt gtcagtgata ttgattttag
     4981 cccctgtaag ttccttgtta atgatgattt tgagttggaa gccttcaccg ctgtttggca
     5041 ctttttccaa aaggcgagtc agttcatagt taccaacctt agtttcaaaa aaggtgttat
     5101 ctttgagggt gaatttttta acagaagggc taagagtgta atcgtaacga caatttttta
     5161 actgaatgat tttttcaaat gccatatggc taacctccga taatttcttt taaggttttt
     5221 gcgagggttt gtaggtcttc aacggtattt tgtggcgaca aactgatgcg aagggattcc
     5281 ttcaagcgtt ctgaatttgc gccatacatg gcttcaagaa catggctgga ttggacaacg
     5341 cctgcagtac aggctgagcc agtagagatt gaaattccag ctaaatctag ccgaaggagt
     5401 aagaggtcat ttttctgacc aggaaatcca atattgagaa cataagggag atgatgtttt
     5461 cctctattca agtaatactg aatgccctcc agctctgcca gaaaggcagt ttctagattt
     5521 tgtacatgtt gaaaatgttc ttcttgtttt tctaggtctt cttttagggc tgcaaccatg
     5581 cctacgatag caggcagatt ttcagttcct gcacgttttt tctgttcctg gtctccgcca
     5641 tgtagatagg aatcaaagtc catgctagat gcgtagagaa aaccgattcc cttaggacca
     5701 tggaatttgt gggcagaagc agtgagaaaa tcaatgccca attcttctga atgaattggg
     5761 attttaccaa tagcctgaac tgcatcaaca tgataggcag cagggtgttg cttgagtatt
     5821 tggccaattt cagcgatggg cagtaggttt cctgtctcat tattggcaaa catggtagaa
     5881 accaaaatcg tatcgtcacg taaagccttt tgaatttgct gggctgtgat ttcttgattt
     5941 tctggctgga taatggttgc ttcaaaccca aagtgttgaa ccaagtaatc aattgtttca
     6001 aggacagcat ggtgctcgat ggcagttgtg atgatatgtt ttccttgttc ttggtgacga
     6061 agacagtagc caatgatggt agtattattg ccttcagtcc caccagaagt gaaaaagata
     6121 tgttgaggtt ttgtccttag taactgggct agttcctgac gggcttctcg caagagtttg
     6181 ccagcttgac gaccatgacc atgaatacta gaaggatttc cgtgggtttc ttgcataacc
     6241 ttggtcatag ctgaaatagc aactgctgac ataggagtcg ttgcagcatt gtccaaataa
     6301 atcaaagaat caccttattt ctttttattg taggcaaaga gtgggctgac tggttttctt
     6361 tcgtgaatac ggacgatagc atcaccaatt aactcactag cagtgatgta gcatacattt
     6421 ttaggagttt tttcttttgt tgctactgaa tcagtcacaa gaatttcttt aatattagta
     6481 ttgtcaagaa gctcagcagc tccctcgacg aagagaccgt ggctagaaac agcataaatt
     6541 tctgtagctc cttcacgttc aacgatttta gaagcttcag agaaggtacg tcctgtattt
     6601 aaaatatcat caatcaagat agctttctta ccttcaacat caccaataat ataaccttcg
     6661 ttacgagttg catcgtcttg agggtagtcg ataatggcga taggagcatc aagatattca
     6721 gccaggctac gcgcacgttt gacacctgaa tttttagggc taacgacaac aacatctgaa
     6781 ccaagcaatc ctttatcgca gtaatgtttt gcgaataggg gaacagtgaa aagattatcc
     6841 actggaatat caaagaaacc ttgaacctga acggcatgca aatcaagagt caggatacga
     6901 tcaactccag ccttaaccag catattggca actagttttg ctgtaagtgg ctcacgagga
     6961 caagcaatgc ggtcttgacg tgcatagcca aaatatggaa ggacaacgtt gatactgtgg
     7021 gcacttgcac gcacacaagc atcgaccatg attaacaatt ccattaggtg gttgttgaca
     7081 gggaaacttg ttgattggat gatgtaaaca tcataaccac ggacactttc ttcgatattt
     7141 acttggattt ctccgtctga aaattgacgt gatgatagtt ttccaagtgg gacaccaaca
     7201 gcttgggcaa ttttttgtgc aatctcttgg ttagagttga gtgcgaaaag tttcatgttt
     7261 tttctatctg acattataga ccgtcctctg taaactttat aaatcctagt tatatttacc
     7321 ttacatatat gaactgggat ttgtgtattt ttatcttttc tattttacca aaaaatggag
     7381 attatttcag ctatttttca tacttttgac aaatcgaacc aattttgaag gagctttttg
     7441 gtaggaaatc tgatttttct ctaaaaattg tcggaaatcc tgtttgcctt gctcatgatt
     7501 ttccacttca agctccaatt cgtaatctgt tatatcaaag tatcggctct gatccagtgc
     7561 catgagacca atagctgttt tcatttcata gcgaagcgtt gttagacaac caagaacctg
     7621 ccagttctta ctttggatac catgtttcgc caattcatcc agtactagtc cttgaggaag
     7681 ttcttcctta ctcagatagt cctcagcatc ttttagttgc aatttttggt tgtattccat
     7741 gtttccaaca ctctgcggga ctttgagtgt caactcagcc cagtcttcaa aggttcgaat
     7801 gcgcatagcg actttctttt ctcgcagttc aaaatcaggc gtgtcgatgt agtaatttgt
     7861 ttgaagaaca ggagtgacac ctgtgaactg gtcttttaga cgattgtatt catctttttt
     7921 caatagtgtt ttcaattcaa tttctaaatg tttcattttt cttacctttt tttatcgttg
     7981 aaagcggatt tatggtataa taagcattgt atttattgta tatgaatctg gagaaaaaat
     8041 caaagatatt tttgacggat aatatgagaa caagggagaa tatatgacct tagaatggga
     8101 agaatttcta gatccttaca ttcaagctgt tggtgagtta aagattaaac ttcgtggtat
     8161 tcgtaagcaa tatcgtaagc aaaataagca ttctccaatt gagtttgtga ccggtcgagt
     8221 caagccaatt gagagcatca aagaaaaaat ggctcgtcgt ggcattactt atgcgacctt
     8281 ggaacacgat ttgcaggata ttgctggctt acgtgtgatg gttcagtttg tagatgacgt
     8341 caaggaagta gtggatattt tgcacaagcg tcaggatatg cgaatcatac aggagcgaga
     8401 ttacattact catagaaaag catcaggcta tcgttcctat catgtggtag tagaatatac
     8461 ggttgatacc atcaatggag ctaagactat tttggcagaa attcaaattc gtactttggc
     8521 catgaatttc tgggcaacga tagaacattc tctcaactac aagtaccaag gggatttccc
     8581 agatgagatt aagaagcgac tggaaattac agctagaatc gcccatcagt tggatgaaga
     8641 aatgggtgaa attcgtgatg atatccaaga agcccaggca ctttttgatc ctttgagtag
     8701 aaaattaaat gacggtgtag gaaacagtga cgatacagat gaagaataca ggtaaacgaa
     8761 ttgatctgat agccaataga aaaccgcaga gtcaaagggt tttgtatgaa ttgcgagatc
     8821 gtttgaagag aaatcagttt atactcaatg ataccaatcc ggatattgtc atttccattg
     8881 gcggggatgg tatgctcttg tcggcctttc ataagtacga aaatcagctt gacaaggtcc
     8941 gctttatcgg tcttcatact ggacatttgg gcttctatac agattatcgt gattttgagt
     9001 tggacaagct agtgactaat ttgcagctag atactggggc aagggtttct taccctgttc
     9061 tgaatgtgaa ggtctttctt gaaaatggtg aagttaagat tttcagagca ctcaacgaag
     9121 ccagcatccg caggtctgat cgaaccatgg tggcagatat tgtaataaat ggtgttccct
     9181 ttgaacgttt tcgtggagac gggctaacag tttcgacacc gactggtagt actgcctata
     9241 acaagtctct tggcggtgct gttttacacc ctaccattga agctttgcaa ttaacggaaa
     9301 ttgccagcct taataatcgt gtttatcgaa cactgggctc ttccattatt gtgcctaaga
     9361 aggataagat tgaacttatt ccaacaagaa acgattatca tactatttcg gttgacaata
     9421 gcgtttattc tttccgtaat attgagcgta ttgagtatca aatcgaccat cataagattc
     9481 actttgtcgc gactcctagc cataccagtt tctggaaccg tgttaaggat gcctttatcg
     9541 gtgaggtgga tgaatgaggt ttgaatttat cgcagatgaa catgtcaagg ttaagacctt
     9601 tttaaaaaag cacgaggttt ctaagggatt gctggctaag attaagtttc gaggtggagc
     9661 tattctggtc aataatcaac cgcaaaatgc aacgtatcta ttggacgttg gagactacgt
     9721 taccattgac attcccgctg aggaaggctt tgaaaccttg gaggctattg agcatccatt
     9781 agatattctc tatgaggatg accactttct agtcttgaat aaaccctatg gagtggcttc
     9841 tattcctagt gtcaatcact ctaacaccat tgccaatttt atcaagggtt actatgtcaa
     9901 gcaaaattat gaaaatcagc aggttcacat tgttaccaga ctagataggg acacttctgg
     9961 cttgatgctc tttgccaagc acggttatgc ccatgcacga ttagacaagc agttgcagaa
    10021 gaaatctatc gagaaacgct actttgcttt ggttaaggga gatggacatt tggagccaga
    10081 aggggaaatt attgctccga ttgcgcgtga tgaagattcc attattacca gacgagtggc
    10141 taaaggcgga aagtatgccc atacttcata caagattgta gcttcttatg gaaatattca
    10201 cttggtctat attcacctgc acactggtcg aacccatcaa atccgagtcc atttttctca
    10261 tatcggtttt cctttgctgg gagatgattt gtatggtggt agtctggaag atggtattca
    10321 acgtcaggct ctgcattgcc attacctatc cttttatcat ccatttttag agcaagactt
    10381 gcagttagaa agtcccttgc cggatgattt cagtaacctt attacccagt tatcaactaa
    10441 tactctataa aaactgtctc agagtataat tattatctta aaggagaaaa ctcatggaag
    10501 tttttgaaag tctcaaagcc aaccttgttg gtaaaaatgc tcgtatcgtt ctccctgaag
    10561 gggaagagcc tcgtattctt caagcaacaa aacgcttagt aaaagaaaca gaagtgattc
    10621 ctgttttgct tggaaatcct gaaaaaatta aaatttatct tgaaattgaa ggaatcatgg
    10681 atggttatga ggtcatcgac cctcaacatt atcctcaatt tgaagaaatg gtttctgcct
    10741 tggtggagcg tcgcaagggc aaaatgactg aagaagatgt acgcaaggtt ttggttgaag
    10801 atgtcaacta ctttggtgtg atgttggttt acttgggctt ggttgatgga atggtgtcag
    10861 gagcgattca ctcaacagct tcaacagttc gtccagctct acaaatcatc aaaactcgtc
    10921 caaatgtaac tcgtacttca ggagccttcc tcatggttcg tggtacggaa cgttacctat
    10981 ttggagactg tgccattaac atcaatccag atgcagaagc cttggctgaa attgccatca
    11041 actcagcaat cacagctaag atgtttggca tcgaacctaa aattgccatg ttgagctatt
    11101 ctactaaagg ttcagggttt ggtgaaagcg ttgataaggt cgttgaagca actaaaattg
    11161 ctcacgactt gcgtcctgac cttgaaatcg atggtgagtt gcaatttgat gcagcctttg
    11221 ttcctgaaac tgcagctctg aaagctcctg gaagtacggt agctggtcaa gcaaatgtct
    11281 tcatcttccc aggtatcgag gccggaaata tcggttacaa gatggctgaa cgcctgggtg
    11341 gctttgcggc tgtaggacct gttttgcaag gtttaaacaa gccagttaat gatctttctc
    11401 gtggatgtaa tgcagatgat gtttacaagt tgaccctcat cacagcagct caagcagttc
    11461 atcaatagtg aaaactataa agcgatatac tatgctatac tgtagttatg aaactatgta
    11521 cgaaaagcac tgccattaat tcctgagaac taaattactg attggtgtca aaaaggaaaa
    11581 cttccaagcg atgatatcct gtctatacac gacctataga aatctgtaat atgtatgtcc
    11641 gtaaaacgat aatttccctt tttgatttta aatgagtatg aaaagagaat tttttcatga
    11701 gaaaacgcat aatattaagt gtcttgacac ctgctattat gcgttttctg gctctttgtc
    11761 aactgtagtg ggttgaagaa aagctaagct cgagaaagga caaattttgt cctttctttt
    11821 ttgatattca gagcg
//
LOCUS       DAVBGY010000055        11791 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVBGY010000055 DAVBGY010000000
VERSION     DAVBGY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11791
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.055"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(<1..809)
                     /locus_tag="VQ620_001647"
     CDS             complement(<1..809)
                     /locus_tag="VQ620_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2764241.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETS"
     gene            1275..1613
                     /locus_tag="VQ620_001648"
     CDS             1275..1613
                     /locus_tag="VQ620_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HET2764242.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            1591..1950
                     /locus_tag="VQ620_001649"
     CDS             1591..1950
                     /locus_tag="VQ620_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764243.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WILPILLMAGMGDVIIN"
     gene            1969..2181
                     /locus_tag="VQ620_001650"
     CDS             1969..2181
                     /locus_tag="VQ620_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764244.1"
                     /translation="MMVVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKKLQIK"
     gene            2596..3060
                     /locus_tag="VQ620_001651"
     CDS             2596..3060
                     /locus_tag="VQ620_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764245.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGGVGCNWWNKRDNIAAAAVSGG
                     MSVYQTYAIKKLIANRGMMSRVVRSAITRYIGGVSMATITNALNIAATVAEVFSLGGA
                     IAYGLDIVDGKFDGYNKINLINSIIIALGMILALILVLSRVYRKYFYRDDDD"
     gene            3217..4275
                     /locus_tag="VQ620_001652"
     CDS             3217..4275
                     /locus_tag="VQ620_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764246.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTVWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNLTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALGDLSKQYLNRNISELQDGSFEGGIQDAVASKQIVVINEFIACLNSRCMPSQYDR
                     FFYKDRPYIFSRKSPIKG"
     gene            4590..5567
                     /locus_tag="VQ620_001653"
     CDS             4590..5567
                     /locus_tag="VQ620_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764247.1"
                     /translation="MKNKRYFFDTILMILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGVAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYISESVREQIDLLEIKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSIRR"
     gene            5944..6318
                     /locus_tag="VQ620_001654"
     CDS             5944..6318
                     /locus_tag="VQ620_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HET2764248.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            6526..7428
                     /locus_tag="VQ620_001655"
     CDS             6526..7428
                     /locus_tag="VQ620_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764249.1"
                     /translation="MIRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTKTLKDKIQKSELSYSDLMSLQLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLMYEMSISNNNNWSNKVDKIRIHLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            7484..7816
                     /locus_tag="VQ620_001656"
     CDS             7484..7816
                     /locus_tag="VQ620_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET2764250.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICVALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGKIAYCIGYGALNRGCDISGNPYPWD
                     GFISATVR"
     gene            7813..8541
                     /locus_tag="VQ620_001657"
     CDS             7813..8541
                     /locus_tag="VQ620_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764251.1"
                     /translation="MNNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IGEDFATKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNRVLALI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKEKEFELFKNSK"
     gene            9593..9832
                     /locus_tag="VQ620_001658"
     CDS             9593..9832
                     /locus_tag="VQ620_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764252.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLETFKVNFCLTDLHHLF
                     GLHKITRDFASHTISVIQTSVFILEDFRNILCTMM"
     gene            10380..>11791
                     /locus_tag="VQ620_001659"
     CDS             10380..>11791
                     /locus_tag="VQ620_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HET2764253.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPVASQSKAEK
                     DYDAAVKKYEAAKKEYEDGKAAQKKYEDDQKKTEEKAEEERKASEEEQAANLKYQQEL
                     VKYIRENDPTKKAEAKKAMDEAEKEYKKKQTEFAEVRAKVIPSAEELKKTRQKAEEAK
                     AKEAELTKKVEEAEKKVTEAKQKVDAEHAEEVAPQAKIAELEHEVQKLEKALKEIDES
                     DSEDYVKEGLRAPLQFELDVKQAKLSKLEELSDKIDELDAEIAKLEKDVEDFKNSDGE
                     QAGQYLAAAEEDLVAKKAELEKTEADLKKAVNEPEKPAEETPAPAPKPEQPAEQPKPA
                     PAPQPEKPAEEPENPAPAPQPEKSADQQAEEDYARRSEEEYNRLTQQQPPKAEKPAPA
                     PQPGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNANGAMATGWLQYNGSWYYLNAN
                     GAMAIGWLQYNDSWYYLNANG"
BASE COUNT         3979 a         1614 c         2243 g         3955 t
ORIGIN      
        1 atagaagttt cctcggttag ctccagaagg gagaaccctt tttggataag tttcgcatca
       61 tccctacaaa tcccaatgcg gacatagcag atagccagcc tatcgtagct tttcttattc
      121 ttggcgtcct ccagtaaagt gtagcagatt tgcttacaca tgtttttatc ttgattgtat
      181 agatagagtg tggaaaggtt taatagaatt gtcattcgca agtcatataa atgttgatag
      241 tttttataga cttctaggcg ttgcaatatt tttccagtga tgagatggag gtattcaatg
      301 ggaaaactga aaagaatggt attgaggatt tttagatcac tctcatacca tgtatcttgt
      361 ttttcaattt ttttccaaag ttttttgaca acctgttctg cgtgttttga tagttcccct
      421 gccccgtgtt gacggagata aataacaact tcaagcatat ctctgatttc ttctataggg
      481 agatcgtggt gggtcttgag gtagtcttgg catttttgaa ataaattgac aagatcgctg
      541 gtcccgataa tggaacgcat attgagatag gtttgcataa tctctgtgcg ttgacttggt
      601 tgataaagtt gacagatata ttcaaactct tcaaaactca tattgatttg acggagaaga
      661 aattccatat tttcatattt tggggttgtc ttgccacttt cgatttttga taagctggtt
      721 cttgagagaa cacccccaca gacctcttct tgggtcagcc cctttgactc acgtatttct
      781 ttatagacct ttccgaaatc ataacgcatg attttctcct ttctgtgaaa aaagttaaca
      841 aacaataaaa ttttgttaaa aatattgtat catagcaatg taaaaagtaa aaaagaaagt
      901 cgaaataaat ttgtgaaaaa agttaacgta attttaaaat gagcttaata agaggtatga
      961 tgtaggtaga aaataaagat aggagaaaaa gtatgaataa aaaaatattt tgtattctag
     1021 tatgtattat atcattgatt agtctggcca taatctttcc atggggctgg tcgatataat
     1081 gtaagggagc ttgagttgtt aaaggaggct tagtccttgg acaaaaatct gctgtactac
     1141 aatgcttgga aagatatcaa gaacaaagaa tgatacgatt gaaaataaac tgattcaaga
     1201 actactgggc agtgattgag gtttgggtga catcaaagaa aagttagatt aattctaaag
     1261 aattgagtat ttctatgtta aaaaaatatc cttgtacgat gcaacatgat cagtcagatt
     1321 gtgctgcggc agttgtttca acagttcttt tatcttacaa aaaggaacta tcaatcatga
     1381 agattcggga aattattggt acagacatgt atggaacgac tgtcagtggt attgtttcag
     1441 gtctgaataa gttgaatttt acagtaaaag ctgttcgagt agcactggaa gatttgactc
     1501 caaaattaac atttcctgcg attcttcaag ttaagaatga tttaggtcaa aatcattttg
     1561 tggtattaca tagtataaag gagaaaataa atggaactcg tattaccaaa taattatgtt
     1621 gtgattgatg aagaagagat gatgtacctt gatggggggg ctatatatat acccaggtgg
     1681 gcaattacag gagccattac tggtgcagca tatgcagcat tagcagcagc aggaggtgga
     1741 ggccttcaac tagttcttgc atcttatgga ttacgctccg cactggtagc tgggattgtt
     1801 aaaggtttag gagtattagg aattcatatt ggaaatgctt ttgcaaatac tgttattaga
     1861 agtattgcat ctgctggaat tggtgctgga gctgattgga ttttaccaat attattgatg
     1921 gctgggatgg gcgacgtgat aatcaattga gaataggata atagtattat gatggttgtt
     1981 ttaatttcag ttattgttgc attcctgtac cataaaatta cagagtatgt caacaacagg
     2041 aaaattgata ttgttatttt tctatcattg ctgatttctc attttatttg gaattactat
     2101 tttttaccaa ttgaaatatt gatagcaatc agtggagcaa atttattatt aagtttttta
     2161 aaaaaattac agattaaata atattattcc atatgacata tcaactaggt cattagattt
     2221 tattgaagat ataaattaaa aacgaggttg ggaaaaaact caatttgaaa gtgaaattga
     2281 gtaaattttt ctattatata acgcatatta tcaagatttt tgaacacctg ataatatgca
     2341 cttttcttat tttaaagatt ttttgccttt cttcatttct tgaatgaatt ggagagatga
     2401 ctttatttag gtgtttgaca ttactataga taggatttag cagtactaat atgaactcat
     2461 attataggtg cagacttaaa ttttggaagc aagctaaagg atatcttatc taggtaacta
     2521 agtgctataa acaaagtcaa cgatcacttg ttaggaaacg atcctaacta tgcttaataa
     2581 aaaaggagaa aataaatgga actcgtatta ccaaataatt atgttgttct tgagcaagaa
     2641 gagatgatgt atcttgatgg gggtggtgtt ggttgtaact ggtggaacaa acgtgataat
     2701 attgctgcag ctgcagtttc tggagggatg tcagtatacc agacatatgc cattaaaaag
     2761 ttaattgcta atcgtggtat gatgagccga gtcgttagaa gtgctataac aagatatatt
     2821 ggtggagtta gtatggcaac tattactaat gctcttaata ttgcggctac tgttgctgaa
     2881 gttttctcat taggaggagc cattgcatat ggtttagata tcgtagacgg taaatttgat
     2941 ggatataata aaataaatct aataaattct attattatag ctcttggtat gatattggca
     3001 ttaattttgg tattatcaag agtgtataga aaatattttt atagagacga tgatgactaa
     3061 agtttttatc aataatttgg gctccttgtc aactgtagtg gattgaagaa aagctaacgt
     3121 ctggggaagg gtttaaagct ttgctttatc aaatattgac ttacagagtt gctctattgt
     3181 attgatgtat tcttagtaaa taatatggtg ttttgaatga aagatgatca gaaatattta
     3241 ttagcgggac tttattcgct tctagttgca atattttatt ttccattgat tgagtccaag
     3301 gggatttttg taagtatatt gatggcggtt ttacttttat atttaatata cttcattgca
     3361 acagtcattc atattgtgat aattaagttt ataagaaaaa aatcttttaa gtatttggtt
     3421 ctatatccat ttacttatga tgggagctgg agatttcaac ctataaactt actctacttt
     3481 ccagaaatgg tgagagatgt cattccgatt aatttagttc aagaatattg ccaaggacaa
     3541 ccttacggac tgttaaaaaa gatgctgaaa agaattcgat tgtcacgaga gatagcttta
     3601 ctcctggcta ccattatcgt ctattttttt acgcatagaa ttctacctct ttcagtattt
     3661 acgttcatat tttcttacat attgctattt gcacaatctt atttgggtgg taataccgtt
     3721 tggattggaa atagaagatt aataatagat gatgaatttg aaaaaatctt gctttcaaaa
     3781 agttatataa aagaaatatc atctgcacga tactcggaat acttgacatg tgaatacaaa
     3841 aaccttacac caatcatatt gttagctatt tttgaaaatt tgcttgactc ctacttgctt
     3901 cagaatcaga gtgaggttga cttagatatt ttttataaag ttttaccttt gctttataaa
     3961 gaaaaatata ctatgggatt caattacttt gtaagtttaa attatttact atataaggtt
     4021 ggatttttgg gaatcattta cgataatgaa gcattaggag atttgtcaaa acaatattta
     4081 aacagaaata tttcggaatt acaagatggt tctttcgagg gcggtataca agatgctgtt
     4141 gcctctaaac aaatagtagt tataaatgaa tttatagctt gtttaaattc cagatgcatg
     4201 ccatcccaat atgatagatt tttttataaa gatagacctt atattttttc cagaaagagt
     4261 cctattaagg ggtgaaaatt tgttacattt ggattaacaa gtgtcctagg gattagaaat
     4321 aactctagta tagctagata tttttgagta actagaaata tcattaggct acccatatga
     4381 ttgccagaaa agatggctga tgaatcttat ttatactgtt gcagtaggca ttgtctagga
     4441 taaataagct atcttgatgg gggaaattgg aataatttta gtagaaacat taaaggatta
     4501 tggacaagta gcgcaggatt aggtgatgca atgtgctaag ttggcttggt gttagcagta
     4561 gtattattta acaatggaaa gtagactaaa tgaaaaataa gcgttatttt tttgacacaa
     4621 ttttaatgat tttactgctt ataagcacga tattttgtgt cagcccagta tttataaaat
     4681 tggacatctt aggaaccccc tctcatgcca tcttaacctt tgtattggcg ataccgttat
     4741 tctatatttt gtctcaatgc ttacatactt tgctattact agtttcctca atcttttgta
     4801 aattaagacc aatttatttt tattttatat ttgtgatcat tataggagct aggaaatact
     4861 atcgaattct ctttcaccaa ctaatgggat ttagtccagg agtagcagtg ttttataagg
     4921 agagtcaaac aactaagaat ctgtttaaat tttactactt cttgtacttt acaacactaa
     4981 tttcatacta ttttttcttt acttttgtgt atgacaaacc gttgcttttg cctcttattc
     5041 cattttctat tatcatagca ttagtacaaa aactttatag aattgagaat caacaattat
     5101 ttctgttaaa atcaaaggta ctaacaatac tggaatctaa gaagaattgt gagtttaatc
     5161 ttcaggatta tcatgaaatt tggaaattgc aatcaaaatc tgagttgcca tgcgtggctt
     5221 tatcttatat cagtttgatc aagccatata tttcagagag cgtgcgtgaa caaattgatt
     5281 tattagaaat taagcgattt aaaaaaataa atcatccaat atcactttat ggtatgttgg
     5341 atgttataaa attaaatcta tatttacggc attataatga aaaaaataaa tatgaatcaa
     5401 tgttaaagaa aattttagag gtaagaccag actttgtttt aatcgagcag aatattgatg
     5461 atagtttaaa tagctcgcaa ccattatctt tatctttagc catttcagaa atccaacttt
     5521 tgcttgaagt gtatatgggg atcaaacatg tttcaattag gagataaaac cagaataata
     5581 tttgccatta taggggcaag ttgcatatta gtcagtatgt atcttattcc acttattaaa
     5641 actgagaata aattttggga aatactttta tttatattag gtgtattgct aattggaatt
     5701 gcgttactaa gtcaaaaaaa ataaattttg aaatagtatt agtgagttcg caatcattta
     5761 ttgctagtgg tttagaactg ttaagtttca aacaataatt gattttaaac cttaggtaac
     5821 ctataaaaac aaataaggta gagaaaggat atcttatcta ggtagctaag tgataaaaac
     5881 acagtcgtct atcacttgtt aggaaactat cctaactatg cttaataaaa aaggagaaaa
     5941 taaatggaac tcgtattacc aaataattat gttgttcttg agcaagaaga gatgatgtat
     6001 cttgatgggg gattttctat tccgagatgg cctgttgcaa cagccattaa tatagctttt
     6061 aatggtgttt taggtggagg agcaatcagt ctagttagaa attatattcg taattatggt
     6121 ttgcggcgag ttacaagcgc aattgctgga gcagctgcaa gatatgttgg ggtacgagtt
     6181 gcaaatagag tggcaggatt tgcactgtct gctattaatg gatttgcagc ttggatgtca
     6241 attggcgatg ctattacaac aatctgggcc aacaatgatg taaataggag agacccaaat
     6301 ttaaacgcct tgtggtaact agatatgggt attaaaatgg aaaaaatttt tgttattatt
     6361 ttttttgtat gtctatttat atcatccata acttttttag cctatgattt tgttagcgaa
     6421 gaaataaaaa aattgattat ttggatgaat gtcgtttttt tgattttaat catagcaatg
     6481 atgatttatc caaaattaag aaaatgataa ggacactgta aaattatgat acgtaaacta
     6541 attgttttct tactaatgct ccctcttttt gtcttgtggc tagagttgca tgtcctagtg
     6601 aataacttac tattgaatct agaatctccc cttgattttg tgattagtat gagtcttgct
     6661 ttctgtagtt tgattttatc aaaaatagtt ttagacatac tatacgcatt gaaagatttg
     6721 tacaagaaag aagctcttat tacgattttt ccttttattt ttataggcag gaagaaagta
     6781 aatgtgaggt tctcgcccta tttttcattc catcgtaaaa gtttgtcgcc tgatgattta
     6841 agatctagga ttatatggag ttttattctt gaaattgcta ttatcctagt atttatttta
     6901 aaaattcctt ttgcaatcat catgcttact acaatatttt tctggactat aatggatatc
     6961 aatcatctcg tttttaataa gacggaattt ctttttaatc aaaataaatg ggaaaaagaa
     7021 gattcgtttg aaagcgattt gacgaaaacg ttaaaggata aaatacaaaa atcagagcta
     7081 agttattctg acttaatgtc tcttcagctg tatgatgcga tgaaccaatc caccttttta
     7141 acggatagtg aactttttga ggatattttg aaaaaaatcg aagattctca taacaccctt
     7201 ctgtgtacag gtcttgtcga gttactgatg tatgaaatgt ctattagtaa caataacaac
     7261 tggtccaaca aggttgataa aattaggata cacctcatca gaatcaatca attagatttt
     7321 ttctattata caagttggtt aaggcaaaat tttgatttct gtatgaatag agagtatcat
     7381 aaaatgaaat ctcggaaatt attactgagt aataaaaaga tagtttaagt tcgtcctatt
     7441 tgtaaaacaa atgaaacaat ttcaactaag gaggagaaaa caaatggaac ttgtattacc
     7501 aaataattat gttgtgattg atgaagaaga aatgatgtat cttgatgggg gagcttattt
     7561 aagcaagcgt gcttgtcaag gaatttgtgt agctttagct atgagttcag gaacttttat
     7621 agcattagct ggagctgcag ttttaaccaa aaaactaata aactatatta aggttggagg
     7681 tcttggaggc tggcttattg gtgcagcagc aggaaaaata gcttactgta ttggatatgg
     7741 tgctcttaat agaggttgtg atattagcgg gaacccttat ccttgggatg gattcatatc
     7801 tgcgacagta agatgaataa tgtagataag attcgaaaaa tacatattat cgtatgttgg
     7861 atgtatatat ttttatcatt cagggcgatt ataaatgaca cggagtattt tttgcttatt
     7921 tttttagctt ttatctactc aatagtatca ctacctctat attctgtgaa aaataaaata
     7981 gtatctatct gtctagccat aaattcgatt ctgttaatga gtttccccat tttaatcaat
     8041 aagtttttcc cagaaagttt tttgacttat accgtattaa taagtgtttt tatcctagag
     8101 ttagcaatct ttcatttaat tggggaagat tttgctacta aattgactaa cgaatataag
     8161 aaaattagtc agtttagaag caaggtgtct caatctcctt ggataaaata tttagagatt
     8221 tctagtttta tattaactat atttccatct attctttacg gtacagttga taatcgtgta
     8281 ttggctctta tctttttgat aaaaatttgt gtagatacta cgataaaatt tttattcatc
     8341 agattatttg acacaagtac tttaatgaag aggagaatat tttttctttt cgcattggat
     8401 gttatagctt acttattttt aggatatctt ttagtgattc aaaaagcagg ctatttgttt
     8461 tcggttttac ttcttttttc taatttttca gttccattta tcaaagaaaa ggaattcgaa
     8521 ttatttaaaa atagcaagta atagaaacat aggttaaatc ttagaacggc ttaaaatcag
     8581 atatgataaa gggaaatagt cttatctagg taactaagtg ctacaaacac agtcgaggat
     8641 cacttgttag gattacattt taatcatgct taataagaat ggagaattcc tatggggatt
     8701 gtattactct gaagtttcga ataccttagt tctcgtgatt ttgagctatt ctaggtttag
     8761 aattgctcat atatatgtat ttgagctgct cgatagttag gacagctcta ttctgagaaa
     8821 tctgagctat caatgcttca gaatagtatt tcgccaatgg tttttattga agaggtgatt
     8881 gttgtatgat agtctaaaat aaaactgatt taatggagta aatggagaat cgttacatga
     8941 aacatttttt tgcaggaatt ggtgcgataa aacttgttgg ctacttattg tatatatgtg
     9001 taggactagc ccctcttttt catatatatg tcataggttc ggaactgagt tttgtgaaaa
     9061 tagtgttatc tattttggga gttatatttg taagtatgtt aactattgct cgaatttatc
     9121 ggacttttct ataaagaata gtttggactt gaactgtatc taaaaacaga ggttggtcga
     9181 ttatcagcct ctttcttgtc gttgaaaaga gccagatata ttgacttttg aaaaaaagat
     9241 gtcataattg agttgggata aacctaaaaa taatatagta gattgaatct ggaatagtcc
     9301 gttatgactg ttaaaatatt tctagaaatt aatttgactt tcctaatcga tttgttcaca
     9361 tcttgtttca atctactata ctttctcgtt aaaccgttcg gcaattcatg gtgacatgga
     9421 cacatagtag taagcacact atgtggcttg gcagagctaa aaactgttcc cttgcgataa
     9481 gcctaataag cacaacatag ggagttagag aagccgactc taatcaacca ctttgggcag
     9541 tagtgagaac tgccccgtgc ttttttattt tgagaaaata tggagtttgt cgttgaaatt
     9601 acttgattgt attttggatt atcaagaaag attcaatgga aaaacatgtc aagtatcaac
     9661 gaattataag tatttagaga ctttcaaagt gaatttttgc ttgactgatt tacatcattt
     9721 atttggctta cacaaaatca cacgagattt tgctagtcat acgatatctg ttattcaaac
     9781 tagtgttttt attttggaag attttagaaa tatcctatgt acaatgatgt aatcgaaagg
     9841 atatctttat atgagtttat cggcgatatt ttctattcta aaataacaag ttgttgtatc
     9901 gtactaagga tttatctaaa aatactatga aattggacgt catatttttt gaggataaaa
     9961 ataaaagatc cgcagtttta ggtttacgaa gagacaaaag cggagtattt aaaccagtta
    10021 ctctacattt tacaagcgct aagaaatatg ctaaagttcg taaaacagat gtgaaagaaa
    10081 tgaaatggct ataaagattg tccgcaggct taagcttgcg ttttttgttg tttaacgaaa
    10141 ttaaaaaaca agctctctca tcggaaatgt ttttatagga atgaaggaag atgatgctat
    10201 gtttaaaaag agaatcgaaa agggcaaaag tagtgtcttt atctttttgg agtagaagct
    10261 tatgatatag aaatttgtaa taaaaatgta atataaaaca tttgacaaat atttacgggg
    10321 ggagtatact taatataagt atagtctaaa aatgactatc agaaaagagg taaatttaga
    10381 tgaataagaa aaaaatgatt ttaacaagtc tagccagcgt cgctatctta ggggctggtt
    10441 ttgttacgtc tcaacctact tttgtaagag cagaagaagc tcccgtagct agtcagtcta
    10501 aagctgagaa agactatgat gcagcagtga aaaaatatga agctgctaag aaggaatatg
    10561 aggacggaaa agctgctcag aaaaagtatg aagatgatca gaagaaaact gaggagaaag
    10621 cggaagaaga aagaaaagct tctgaagaag aacaagctgc aaatctgaaa tatcaacaag
    10681 agttggttaa atatatacgt gaaaatgatc caacaaaaaa agctgaagct aagaaagcaa
    10741 tggatgaggc tgagaaagag tataagaaaa aacaaacgga atttgctgaa gttcgtgcaa
    10801 aggtaattcc tagcgcggaa gaattaaaaa agactagaca aaaagcagaa gaggctaaag
    10861 caaaagaagc agaacttact aaaaaagtag aagaagctga gaaaaaagtt actgaagcca
    10921 aacaaaaagt ggatgcagaa catgctgaag aagtcgctcc tcaagctaaa atcgctgaat
    10981 tggaacatga agttcagaaa ctagaaaaag ctctcaaaga gattgatgag tctgattcag
    11041 aagattatgt taaagaaggt ctccgtgctc ctcttcaatt tgaattggat gttaagcaag
    11101 ctaaactatc aaaacttgaa gagttgagtg ataagattga tgagttagac gctgaaattg
    11161 caaaacttga aaaagatgta gaagatttca aaaactcaga cggtgagcaa gctggacaat
    11221 accttgctgc agctgaagaa gacttagttg ctaaaaaagc tgaattagaa aaaactgaag
    11281 ctgaccttaa gaaagcagtt aatgagccag aaaaaccagc tgaagaaact ccagctccag
    11341 caccaaaacc agagcaacca gctgaacaac caaaaccagc gccggctcct caaccagaaa
    11401 aaccagctga agagcctgag aatccagctc cagcaccaca accagagaag tcagcagatc
    11461 aacaagctga agaagactat gctcgtagat cagaagaaga atataatcgc ttgacccaac
    11521 agcaaccgcc aaaagcagaa aaaccagctc ctgcaccaca accaggttgg aaacaagaaa
    11581 acggtatgtg gtacttctac aatactgatg gttcaatggc tacaggttgg ctacaaaaca
    11641 acggttcatg gtactacctc aacgctaatg gtgctatggc tacaggttgg ctccaataca
    11701 acggttcatg gtactacctc aacgctaatg gtgctatggc tataggttgg ctccaataca
    11761 acgattcatg gtattaccta aacgctaacg g
//
LOCUS       DAVBGY010000056        11731 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVBGY010000056 DAVBGY010000000
VERSION     DAVBGY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.056"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(<1..367)
                     /locus_tag="VQ620_001660"
     CDS             complement(<1..367)
                     /locus_tag="VQ620_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2764254.1"
                     /translation="MTIEPGQRIALVGQSGSGKSTLSKILSGLYKIDTGKILFDGVNI
                     NQIDKKILSQNLGVVPQDSFLLNRSILDNITLKNEVTSQKIEEVCKAVQIYDEIMAMP
                     MKFNTIISEMGSNISGGQRQ"
     gene            complement(536..1849)
                     /locus_tag="VQ620_001661"
     CDS             complement(536..1849)
                     /locus_tag="VQ620_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2764255.1"
                     /translation="MTRKVPNIEQMSQIECGLCCCLSILHFYKSKETLLDLRRDIEKG
                     RDGYSIGDLKQLLNKRNFDTGSYQVKDVNKISELPLPLIAFWDNQHYVVIYKVKKNKV
                     YIMDPAKGYINYEFKEFSKHFSNIVLLSFPNENYQSLKSQFPSPWIRVFSSFSKVKGR
                     LILTLLFSIISYLIILSVPVMTSKFINSALGNTFSFQTSFLILFSLLCLYLISILARS
                     MGILFSNIFFSRDIESFTFKHLLKLPYSFFELRAKGDILYRISSLSGFRELFTNQVVG
                     GVVDIGTILSVVIYMFLSSKTLSIIALILSLINFLFLFSTRKIMYDTVNRELQEQSLI
                     YSVETEALNTISSIKISGLEDEIYENWSKYLKNVLTKYKKRSIVHILYNSATNVFQLF
                     APIIILIFGLDNVLNGKILLGEVVAFQTMASILFSSEISIFNAYT"
     gene            complement(1842..2390)
                     /locus_tag="VQ620_001662"
                     /pseudo
     CDS             complement(1842..2390)
                     /locus_tag="VQ620_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059560.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2471..2533)
                     /locus_tag="VQ620_001663"
     CDS             complement(2471..2533)
                     /locus_tag="VQ620_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HET2764256.1"
                     /translation="MKKISKFLPILVLVMDIIII"
     gene            complement(2702..3690)
                     /locus_tag="VQ620_001664"
                     /pseudo
     CDS             complement(2702..3690)
                     /locus_tag="VQ620_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050775.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3639..4766)
                     /locus_tag="VQ620_001665"
                     /pseudo
     CDS             complement(3639..4766)
                     /locus_tag="VQ620_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423473.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
     gene            complement(4929..5468)
                     /locus_tag="VQ620_001666"
     CDS             complement(4929..5468)
                     /locus_tag="VQ620_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HET2764257.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNIEYIKARNIKISSDSELESDVDGDKSDNLP
                     VEIKVLAQRVEVFSKPKED"
     gene            complement(5580..6434)
                     /locus_tag="VQ620_001667"
     CDS             complement(5580..6434)
                     /locus_tag="VQ620_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET2764258.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNLTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            complement(6492..7250)
                     /locus_tag="VQ620_001668"
     CDS             complement(6492..7250)
                     /locus_tag="VQ620_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET2764259.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYALS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVVF"
     gene            complement(7312..7500)
                     /locus_tag="VQ620_001669"
     CDS             complement(7312..7500)
                     /locus_tag="VQ620_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET2764260.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            complement(7822..8388)
                     /locus_tag="VQ620_001670"
     CDS             complement(7822..8388)
                     /locus_tag="VQ620_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764261.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKTYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            complement(8656..10335)
                     /locus_tag="VQ620_001671"
     CDS             complement(8656..10335)
                     /locus_tag="VQ620_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET2764262.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCNHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKVDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            complement(10427..10588)
                     /locus_tag="VQ620_001672"
     CDS             complement(10427..10588)
                     /locus_tag="VQ620_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764263.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            complement(11272..11586)
                     /locus_tag="VQ620_001673"
     CDS             complement(11272..11586)
                     /locus_tag="VQ620_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764264.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            complement(11552..>11731)
                     /locus_tag="VQ620_001674"
     CDS             complement(11552..>11731)
                     /locus_tag="VQ620_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HET2764265.1"
                     /translation="KELNKEVAFYLTSIIRQALKNTEYKDQISSTVLPDIKIKLPIDS
                     RGTPDWNYMERYRDR"
BASE COUNT         3619 a         2307 c         1539 g         4266 t
ORIGIN      
        1 gttgcctttg cccacctgaa atatttgacc ccatctctga gatgatagta ttaaatttca
       61 tcggcatagc catgatttca tcatagattt gaactgcttt acaaacttcc tctatctttt
      121 gtgaagtaac ttcgttcttt aaagttatat tatcaagaat acttcggttc aataaaaatg
      181 aatcctgtgg aactactcct aaattttgac ttaatatttt tttatctatt tgattaatat
      241 ttacaccatc aaataaaatt tttcctgtat caatcttata taatcctgat agtatttttg
      301 ataacgtact ttttcctgac cccgattgac cgactagtgc aattctctgt cctggctcaa
      361 tagtcaaatt taaattttct attacaggag ctgagtcttt ggaataagag aaagataggt
      421 ccttaatatc tattctacct tctaaagaac actttttaag accattttca acattctctt
      481 cattttcaag ccagatatca tttactctat ttaagtatcc tgctgccaaa atatactacg
      541 tataagcatt aaatattgag atttctgaac taaataaaat tgatgccatc gtttgaaatg
      601 cgacaacctc acccaataaa atttttccat ttaaaacatt atcaagtcca aaaatcaata
      661 taattatagg agcgaacaat tgaaatacat tagtagctga attgtataaa atatgcacaa
      721 tacttcgttt tttatacttt gtcaatacgt tttttaaata cttagaccag ttttcatata
      781 tttcatcttc taatcccgat atttttattg aagaaatagt atttaatgct tctgtttcta
      841 ctgagtaaat caatgattgt tcttgtaatt ctctattaac cgtatcatac attatttttc
      901 tagtactaaa cagaaacaaa aaattgatta aactcaatat aagagctata attgataatg
      961 tttttgaact aagaaacata tatattacaa cacttagaat agtcccgata tctactactc
     1021 ccccaacaac ttggttagta aatagctccc taaaaccact aagactagaa attctgtata
     1081 aaatatctcc ttttgccctg agttcaaaaa aactatatgg tagtttaagc aaatgcttaa
     1141 atgtaaaact ttcaatatct ctactaaaaa atatattaga aaatagaatt cccatactcc
     1201 tagccaagat agaaataagg tacaaacaaa gtagagaaaa taaaattaaa aacgaggttt
     1261 gaaaagaaaa tgtattccct aatgcagaat taataaactt tgaagtcatt actggtacgg
     1321 aaagtattat taagtaactt attattgaaa atagtaacgt caaaatcaat cgacctttta
     1381 cctttgaaaa actagagaat actcttatcc atggagaagg aaactgcgac ttcaaagatt
     1441 gataattttc gttaggaaat gatagcagta ctatattact aaaatgtttt gaaaactcct
     1501 taaactcata atttatatat cctttagcag gatccattat atagacctta ttctttttta
     1561 ctttgtaaat aactacataa tgttgattat cccagaatgc tattaatggt aacggaagtt
     1621 cactaatttt atttacatct ttaacttgat atgagccagt atcaaaattt cgtttattca
     1681 ataactgttt caagtcacct atactataac catctctccc tttttctata tcacgtctta
     1741 aatctagtaa tgtctctttg cttttataga aatgtagtat ggacaagcag caacataaac
     1801 cacattctat ttgagacatt tgctcaatat taggtacttt tctagtcatc atgtcctctt
     1861 aactccagta ataaaacatt aagtaatcta tcatcttgtt ttcgagatat ttcatagaca
     1921 actccagata ctcctagcat aaaagaaaat gtattcatag tattcaaatc cgtacttaat
     1981 tctggtttga taacctgaga aacgacatta tcaaaataaa aatctataga ttcttttatt
     2041 aataggtttt cttcatcttt caatattgca tcaagacaac tgatagtacc tacagaaccg
     2101 tgacataaat tcaattctct tattttttct acattagtta atacattttc aattattgat
     2161 aaatcattat tcccaatatt agttttatcg aaaatttctt ttctagctaa taaaatgccc
     2221 ggagctccat aacaccaaaa attaggaaag gaatcattat gatttcttca tatgtctttc
     2281 caatttttta atatattatt gaaacattta tcttctttat atagtaattc ctctatacat
     2341 tttaatatat ctgtactagg attaatttca taataatatg ataattacat tgctactcca
     2401 gaataatcgt gtgctaaacc tgttactgtt atattatcta attgtgaaat acaataatta
     2461 atacactttt ttaaatgatg ataatatcca tcaccaaaac caaaatcggt aaaaattttg
     2521 aaattttctt catacttgga cttcctttcc gtttttattt attgtacaat aaataaaaac
     2581 ggatttaatt tttgttggga aaatgggaaa ttatgaaatc aaaacttggc atcacactta
     2641 gaaaagttcg taaaggaaaa caaattagtc tatgctctgt agcagatgaa catctttcaa
     2701 aatcacaaat atctcgtttt gaacgtgggg agtcagagat atcttgtatt cgactaatca
     2761 atattttaga caaattacat attactttgt aggggagttt ttggctatcg ttgctcaatt
     2821 gtctgattaa ggtcctaccc ttgatgaaac aattattatc catgttttct ttattataga
     2881 caaagtaaga agacgtttct cgaatgtaga ctttatattt tttatgattt tcttcttcca
     2941 taatatccaa ttgatagttg ggaatgaaaa taagaccgct ctgtttgaca ccgaaagaca
     3001 ccttgatata gacgccctta tcaactagct tctctatttg gttctctgca agttccactt
     3061 caaattcacg aacggtatct catttttcct taaatgtctt aaaggcttcc tcaatctctt
     3121 cagtggatac tttatcctta tctcgttctt cttggaaagc atggtactgt tcctgtaaat
     3181 tctctaatcc ttctgaagca acgacttcct tatttttaaa ataatcttga aaaaatttga
     3241 catcatataa tttcttatca cttatttttt gatgacccaa acttatcttt tgattatttt
     3301 cttccaggat aaaagttaca ttttttgttt taagtcaatg gttagattca attcttttgc
     3361 ttttgttatt aaatcttcta aagaattgac acggtttaac aaaaattcta aacgactttc
     3421 aatctcttgc ttagcaaaat gcgttctaaa aaattcttca tcatatagat ctcgtttgct
     3481 gagttggcgc cctcgaattg gttttatcat cgttctatct gtcatcaaaa aacggctatg
     3541 cttttgacta aaatcaatct gaacatgcaa ctgctttgct ttctctaaaa aatcatcaaa
     3601 cgatttagat tgctgaagca aaaaataaag acgttgtttc aattcaaatt tatgactaga
     3661 ttccttatat tttttataat ctcgaaaaga tcaggatttt tagctaaaac tgccttacga
     3721 aaatccttcc agaactgatg gtcaatctca ttagccacat ccaaacgcca agcatcgata
     3781 ttaaactctt caatccaata agtcgcaacc tttaaaagat aattcttgac ctctggattg
     3841 gctgtattta gcttaggcat atagtcctcg aaaccaaaaa catgataggg taagtctctc
     3901 ttattaacta gcttttcagt tgtcaatggg aattgttgaa tatggaacca atccttataa
     3961 gcagactgtt caccattttt gacgacattt tttcattgaa gagattgcga accaatatga
     4021 ttaaataccg catccagcat gactttcatg ccacgatgat gcgcttgatc caccagttcc
     4081 cgaaaggtct ccttgtctcc aaaataacgg tcaatttcaa agtaatctgt cgtattgtac
     4141 ttgtgattac ttgtagattc aaagatggga caaagatata gtccagtaat acccaagtct
     4201 tgcaagtaat tcatatgatc aataatcccc tgtaaatcac caccaaagaa atcatcgctc
     4261 ttaggtgtga cagatgaatc ccagtctaaa gtcccttctg ggtttaatag agcattgcca
     4321 ttggcaaatc tttcaggaaa tatctgatac cataccgtat ttgaaaccca gtcaggaacc
     4381 ttgcaggcat caatctcatg aagataaggc aacttaaatc cattcccaat tgcatgaaga
     4441 ttttctagag aattttccac acacccttta tcgccataca aaatattttg accttctgta
     4501 tctctgagct caaagagata ctggatacgt gcaaagtcaa ctgacacttc aacctgccaa
     4561 tggtcaaata aggtaccaga agttatcttg accatttctt ttgtatcctg ataaaactct
     4621 tccataaaga taaaagggtc cccatagtgc aagttgatgc tttcaatgtc ccctttctta
     4681 gttcgaattc gaatatggag tttcttatcc ttataaagat aggcatactc cgactcaggc
     4741 ctatggtaaa tagcactcaa ttccatcaat ttgtctccta aagtattcat tcaaatttta
     4801 cgcaaacgtt ttcaaactca ataaatccag tatactattt ttgtaaacta tttgcaagga
     4861 atgtccgttg ttaaaggaac tatcttttaa agtatacaaa aagccttaaa aaaggctttc
     4921 tctatatact aatcctcttt cggttttgaa aatacttcta ctcgctgagc taggactttg
     4981 atttctacag gtaggttatc tgatttatct ccgtcaacat ctgactccaa ttcactatct
     5041 gaagagattt taatattacg cgctttgata tactcgatat tatcatttgc tttaccgata
     5101 tcaatcttat tggttaaatc aaaatccagt tcgtcaattg cgccatcgat gtcttgattg
     5161 atttccaaaa gttttgtaat gaggttaccc gtaccgcctg ggataatccc taacttagga
     5221 atgtagtctc tctcatcaat acctgaaatg acttcattga cagttccatc tccaccaaac
     5281 acaaccactg catcatactg ctcacgagaa gcttcttcag caaaatgtgt tgcatccagc
     5341 gctttttcgg taattttggt ttcaacatat tcaaagtatt cttttgcttt attctccagc
     5401 ttttctttgt aatccaaagc cttctcgcca ccagaagtag ggttgataat taccattgct
     5461 tttttcattg attttatcct taattttaaa cagaaatgtt tacatttcgt cgtatgcaag
     5521 taaatgtaat cctattatac aatgaaaata cagaaaagag aaatctgacg tactggagat
     5581 taatacgctt ttattctatt ttcccatcgc ctaactacat cctttaaggg ttcatccaag
     5641 taagaatagg ccttatcctt gatccaatca ggaataccgt aagctgcctc tgctatgtta
     5701 caagtgattg ctgcgagagt atcactgtcg ccaccaagtg agatggcatt tcttatcgca
     5761 tcttcgaagt ctctactttc aagaaaggcg ataatggctt gagggacagt ttcctgacat
     5821 gtttcgttaa aacgatagtt aggacggatt tcatctaaag tttgagatag attgtaatcg
     5881 tattcttttt ctatataatc cttaattcgt cttttacact ctgtaagatt gtcgttaatt
     5941 gattgctcgt actctctaca ataaccacca aagtaaaaac gacatagaaa gatagcatca
     6001 gctgtagcca tagcgccttt gacaccttct ggatgattat gagttacctc tgcagaaaga
     6061 ctcgtaagtc ctctagatga tggccatata ccagttttcg cataaaaacc acagtccatg
     6121 atccaagcac atggagaaat acgcatagct gatccattcc caaagctatt ataaggctca
     6181 cggttatcgc tgtttagcca tgcattaaac cgagcaccgt aatcagcatt cggatacatt
     6241 ctgccatatt tcttcatcgc gtcaatgaaa tcgtcttttt gtccaccatt cataattgct
     6301 tctgcaacag cacaggtcat aaccgtgtca tctgtaaaaa agcagtcctt ccgaaataaa
     6361 ggaaagtcct ttgttttgat attgttccat tcgtaaacag aaccgacaat atctccaata
     6421 attgctccaa gcatcagatt cctccttgtt cattttgatg ctttttatat tggttatata
     6481 ccatatttat tttagaaaac aacatcctgt tggattttaa aaatttcatt tttttcaaaa
     6541 tagggtttta ccatttcttt ccacctagct ctatgaaaat tgattgattt taaaggagat
     6601 agggcataat ttcccaatgc ataaccatca tttacttcaa caacaagtgt tctgccatcg
     6661 cgagtaacac cgatatctag tccataagct attggcgcat ctttccaaca tgatatcgct
     6721 tcatcaatta cacttgcatc aaattgtgca tgataatcac ctgtataggg tcgaacatct
     6781 aatacgcgac catctaacac aaaacaacgc cattcagcta tgaattctac aacctcacta
     6841 atccatatag gatagtcgaa aggtagacca atacctatta aatcatgggt tccattaaca
     6901 actcttccag taaagacttt tgaaccagct ttaggcttaa taaattttcc ccaattatca
     6961 ggtatattca caatctctcc taaaatacca gcataaatct ttcgaccata aaactcttta
     7021 agctcaatag gatagtcata aaccggaacg tttaagccca tcatttttag taatgctcta
     7081 gtctccatta tataatctac aactatatct tcacttgtta actcttttat ttcagaaaaa
     7141 gattgatata aaataacttc ttctccttgt aagtaggcac ctacttgagc attgtattta
     7201 ttaattgaaa cctcacttgg taatttactt tgtctaatat aaacaaccat ttcatcactc
     7261 ctatatcact agtgttacac tgatttgtaa aaaataatag caattttgct cttatttttt
     7321 tgagtaaata gctcccataa tatcatcgaa ataatcaacg gtatttagga gtaattcaat
     7381 aacctgggac tttgttagtc gcattcccct tctatctcta gcatcttcta ctaaattttc
     7441 aagtttctct agatttttat catccaagct aatcattatt ctatttttat cggttgccat
     7501 tttcatcacc tcaagttaat tctatcacag gtgtaacact tgtgtcaact ggcttttata
     7561 atacattagt ttaaaagtgg agaggatttt taacacagta actttaaatc tttggtatta
     7621 aaaaattttc acaatattta tagaaataaa atctgtctca aatcagttat caaatctagt
     7681 ataaattatg agcggctact ctaatacttt ccctctaaac aagaaaaaga cttacactca
     7741 aggattttct tccccccctt cgttataacg ttttgactct tttactagca aaggtatata
     7801 ctcacaagga actttggttg actattgaat ctctccaact tcttctttaa catatccttc
     7861 tacatcttca atctctacaa acattgggtc taagtgacac aagaaatgcc aaacttcgat
     7921 cccttttttt ctgtaaagaa tcgcttcacc gtcttcactt ccgaaaaagc ttctgtcgat
     7981 ttcatatccg cggctttcta agaagtcttt tgctttacga tagttcgttt ctcttgtttc
     8041 gacataggtt ttaacttcat ggttgttaac gacatatgca tcaatttttg aatatccttc
     8101 gatcactcta tcatttttga gggataaatt tgaaatctct ttccaaataa tgtttacatt
     8161 ttcctcagga tcgaacataa atttagataa aggaacaata tttccgttaa aaataatttc
     8221 catataatcc ggtatgtttt taggattaaa atactccact tcaaaaccat cttctgtttc
     8281 cagagtgtat cccgggattt gagctacaaa ggctttccca tcttctatgg aatcaaatgc
     8341 tactaaatct ttagaataat cattttggta caattccaat ataaccatcg ataatctctc
     8401 cattttcatt atcaggctaa tgtaaataag cacgtcacct gaccaattca ggctctctgt
     8461 atcatctcat catatttcct acttacttta cgagtcctat acccagaaca caccttatcg
     8521 accttcggtc tcacctcgtt gcattggctg aacatctact tttacttcgc tgatgcttca
     8581 actcgtacaa gcagtgatac cgcctcagcg tgatgcgtca gtgggactca aaaggttcgg
     8641 ggaacctttt gaggattaac tacgtttctc taataaactt acacattcaa cttgttcatc
     8701 attgtccaaa cctatgttga gattttcttc tataattggt agcttaaaag taatggattt
     8761 tagccattgt ccgttagatt gtttttcttc ataaacttga atttcagaaa tcaaagctga
     8821 aattaactgc ctacgctcta catcattcat gactttatag agcttatcaa aatagatcag
     8881 aaccttatat atgttatctc ctgtaagctt ttcagcttca atagtctgtt tctttgcttt
     8941 cgcatcaatt agtgatgatt ctaattcatc tactttgtca tacatacgat atagtctatc
     9001 atctaaatcc tgtttccttc tcttataatg cttatcttca acatctaaat tatctatttc
     9061 ctcaattagc ttaaactttg tagaatgact ctttctcaat tccttttggt aattatctat
     9121 ttctttttct atttcagagg tatccacctt catgttgatt ttttcttgca tcatagaagc
     9181 aaatttcgga ttacttacta tcttgacaat cacctctgca acagcatcat ctaacaattc
     9241 ttctctaatt tgcttactga atgtacactt atgacctctt atcatctgcc tatggttaca
     9301 accatagtaa taaaaatctt tatactttgt gccatctttc tttttcttga tacacttgtt
     9361 cccaaacatt cccactccac atatcgggca ttttacaatt ccagaaagca agtgtgtgcg
     9421 tgtatctttt cctttattca catgctcata tttctttgct tgagatttta gcttaacctg
     9481 agcagcttgc caaacttcat cggaaactat agcttcatgt atcccttcag atattagata
     9541 ttcatcttgt tcaacctgct tatattcatt tcttgtacca tgaacttttt ctaaagttct
     9601 tcttccaaat gctattttcc cattatatac aggattcttt aatatctttc ttataagacc
     9661 tgcatcaaac aaaggattct taccattctg tcttgggatt tttctaattc catgattctc
     9721 taagtattta gatatcccat tggctcctat cgtagtattt acatactggt cgaaaatcgt
     9781 tcttattgca actgcctctt cctcatttat aaacagcttg ccgtcttcaa gtttatatcc
     9841 atacggagca aagccaccat tccattttcc ttcccttgct ttttgaatgc gaccttccat
     9901 tgtttgaata cggatgtttt ctctttctat ttcagccaca gctgataaaa cagaaatcat
     9961 tagtttccca gcatctttag atgaatcaat gccatcttca acgcagataa gattaactcc
    10021 ataatcctgc attatctgaa gtgtagaaag aacatcagcg gcatttcttg caaatcttga
    10081 taacttaaac acaagaacaa aagatactcc atcttttcca gattttatat cttccatcat
    10141 tcgattgaac tgtattctac cttcaataga cttgccagac ttcccggcat cttcatactc
    10201 tccaacaatt tcataatcgt tgtaaatagc aaaagctttc attcgtgatt tttgtgcctc
    10261 taacgaatac ccctctatct gtattgacgt agatactcgt gtatagaggt atacttttat
    10321 tttttctttt gacatagtat taatctcaat ataatttttc tatatcatat ataatttttt
    10381 taatttaagt ttggactatc atttcaagta tattataaca cttttattag tccgtctcaa
    10441 tttgtgtttt tgccatgtca aaactatttt tcatctcttg attttttgct ggcgttggat
    10501 cgggtagatt atctaaatct aaagcaccag catattttgc aatcagattt gctattaaat
    10561 cagccaatcc attccagtca ttgtccaata tatacctcct ctaaagtttt atatctaata
    10621 attatttgtt taattaagtt ttttgacatt gacaagtgct ttggattagc aacataggaa
    10681 tctcacctcc gcctctattc cggatgagcc ggcttcaacc ttagaagtat cattatcctc
    10741 attttcttca tagcggatag ggtatccctc cctatattca aactcttact tatcgctcac
    10801 tttcttttta cttagcagaa ctttttttgc cgaattattc agccgaaaga tcttgacgga
    10861 taggttatta cgctccaaaa ataattaacg tcttgtcttg gtctattcaa ttgttaaggt
    10921 tcaaaattta tcgagagtta ttaatctttt taaaatttga ccatcagaaa atatttatct
    10981 taatgtaaca aaattctata aattaccctc ttatacttaa cagtgaaaag aagtctttct
    11041 tggtaaccaa ttttgaaata gaatttgctt atataaaaag gtcaaattcc cactgcataa
    11101 gtagcagtga aaattagacc ctcttggtaa ctgtcatcta aaagtcttct agcatttctt
    11161 taattttcta atcgccttta aagtgtcata tttatatttt tttatctctg gcatactttg
    11221 ataccttttt agacttaaag tctttaatag tgcctttcca cctcttttta tctataaaga
    11281 ttctcctaca tcataattca tttttttatt taaacctttc tgtcttagtt tgtctttatc
    11341 ttcttcatac cattttaaga ttgtcacata gtggttttga taggtcttac cactgctttc
    11401 catgtatctg gatagtttat ttatcattat atctgtgtgt gagtttaatt tttcttttag
    11461 atttttatat tcttctttgc ttaaccttac atttttgaat tctccataaa aaatgggggt
    11521 ggacttttta tctatctctc cctctctctc tttatctatc tctatatctt tccatgtaat
    11581 tccaatctgg agtacctcta ctgtctatcg gtaatttaat tttgatatct ggcaatactg
    11641 tgctagatat ttgatcttta tattcagtat tttttaaagc ttgcctaata attgaagtta
    11701 aatagaatgc tacttcttta ttcaattctt t
//
LOCUS       DAVBGY010000057        11405 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVBGY010000057 DAVBGY010000000
VERSION     DAVBGY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.057"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(52..2049)
                     /gene="metG"
                     /locus_tag="VQ620_001675"
     CDS             complement(52..2049)
                     /gene="metG"
                     /locus_tag="VQ620_001675"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET2764266.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDKAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEYDGKLTLLTVDPAVPNGSVIG"
     gene            complement(2173..3432)
                     /locus_tag="VQ620_001676"
     CDS             complement(2173..3432)
                     /locus_tag="VQ620_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2764267.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKKSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(3434..4135)
                     /locus_tag="VQ620_001677"
     CDS             complement(3434..4135)
                     /locus_tag="VQ620_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2764268.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(4119..5318)
                     /locus_tag="VQ620_001678"
     CDS             complement(4119..5318)
                     /locus_tag="VQ620_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET2764269.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILIPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            5604..6950
                     /gene="gor"
                     /locus_tag="VQ620_001679"
     CDS             5604..6950
                     /gene="gor"
                     /locus_tag="VQ620_001679"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HET2764270.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            7147..7683
                     /locus_tag="VQ620_001680"
     CDS             7147..7683
                     /locus_tag="VQ620_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HET2764271.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            7734..7820
                     /locus_tag="VQ620_001681"
                     /pseudo
     CDS             7734..7820
                     /locus_tag="VQ620_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(7889..8104)
                     /locus_tag="VQ620_001682"
     CDS             complement(7889..8104)
                     /locus_tag="VQ620_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HET2764272.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            8202..8522
                     /locus_tag="VQ620_001683"
     CDS             8202..8522
                     /locus_tag="VQ620_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764273.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(8553..8891)
                     /locus_tag="VQ620_001684"
     CDS             complement(8553..8891)
                     /locus_tag="VQ620_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764274.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(8903..9622)
                     /gene="trmD"
                     /locus_tag="VQ620_001685"
     CDS             complement(8903..9622)
                     /gene="trmD"
                     /locus_tag="VQ620_001685"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET2764275.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(9612..10130)
                     /gene="rimM"
                     /locus_tag="VQ620_001686"
     CDS             complement(9612..10130)
                     /gene="rimM"
                     /locus_tag="VQ620_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET2764276.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKRYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(10161..10949)
                     /locus_tag="VQ620_001687"
     CDS             complement(10161..10949)
                     /locus_tag="VQ620_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2764277.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
BASE COUNT         3280 a         2505 c         2078 g         3542 t
ORIGIN      
        1 attgagtata aataaaaaaa cagaactagt ctgaactagt cctgtctact tttacccaat
       61 cacacttcca tttggtacag ctggatcaac tgtgagaagg gttaatttgc catcatattc
      121 agctgataga atcataccct ggctgacata ttttttcatc attttacgtg gtttgaggtt
      181 agcaacgatt tgaactttct tgccgaccaa ttcttgttca tttggatagt attttgcaat
      241 tcctgaaaga atctgacgat cttctccatc accagcatcc aagcggaatt gaagcaactt
      301 atctgaacct tctactttag acacttcttt gacttctgcg acacggattt caaccttgtc
      361 aaagtcttca aacttgattt catccttgtt tagtttgagc tcaacttcgt ccggattcca
      421 ttctttttcg actgctggtt tattgccttc catttgttcc ttgatatagg cgatttcttc
      481 ttccatattt agacgtggaa agataggtgt tcctttggca actacagtca catctgctgg
      541 gaagtcagcc aaactcaagt tttcaagact agaaacttct tccaaaccaa gttgagtcaa
      601 aactgcacga ctagtttcca tcataaatgg ttcaatcaag tgagcaacta cacgaatgct
      661 ggctgccaag tggctcatga cacttgccaa ttggtcacga agagctttat ccttggccaa
      721 gacccatggt gcggtctcat cgatgtattt attggtacga gagatcagag tccagactgc
      781 ttcaagcgca cgtggatagt caactgcttc catgtgtgta tggaagtctg cgattgattg
      841 ttctgcaacc tcagcaagaa catgatcaaa ttcagtcaca ccttctacat aggcagggat
      901 ttgtccatca aagtacttat taatcatgga aaccgtacgg ttaaggaggt tcccaaggtc
      961 attagccaat tcatagttga tacggccgac atagtcttca ggagtaaagg ttccgtctga
     1021 accaactgga aggttacgca tgaggtagta acgaagtgga tctagtccat aacgctctac
     1081 caacatttca gggtaaacga cattcccttt tgacttagac atttttccgt ctttcatgac
     1141 aaaccaacca tgggcaatca aacgatcagg taatttaaca tccaacatca taagaaggat
     1201 tggccagtag atagagtgga agcgaaggat atcttttcct accatatgga agactgttcc
     1261 attccagaac ttgtcaaagt taccatgttc gtcttgagcg tagccaagag ctgtcgcata
     1321 gttaagaagg gcatcaatcc aaacgtagac aacgtgtttt ggatttgatg ggacaggcac
     1381 tccccatgta aaggttgtac gagataccgc caaatcttcc aagcctggct cgatgaagtt
     1441 gcgtagcatt tcattaaggc gaccatctgg cgtgataaat tcaggatgag ctttgaaaaa
     1501 ttcgaccaaa cggtcttggt atttgctaag gcgaaggaag tatgattctt cagaaaccca
     1561 ttcaacctca tgacctgatg gagcaatacc accagtcaca tttccagctt catcacggaa
     1621 aacttctgcc agctggcttt ctgtaaagaa ttcttcgtct gatactgaat accaaccaga
     1681 gtattcaccc aagtagatat catcttgagc aagtaagcgt tcaaagacct gtgcgacaac
     1741 tttttcatgg tagtcatcgg ttgtacggat aaatttatcg tatgagatat ctagtaattg
     1801 ccagagttct ttaactccaa ccgccattcc atcaacatag gcttgaggtg taataccagc
     1861 ttcttccgct ttctgctgga ttttctgacc atgttcatca agacctgtca gataaaatac
     1921 atcgtagccc atcaggcgtt tgtaacgtgc taggacatca catgcgatag ttgtgtaggc
     1981 agaaccgata tgaagtttcc cagatggata gtaaatcggc gttgtaatat aaaaattttt
     2041 ttcagacata atttttcctt tccaggcaaa tgaaacctgt ttttctaaca cttcattata
     2101 tcacattttt aatgaatttc gatagggaaa tccatacaaa aacaagatag acgagtgtcc
     2161 atcttgttga tctcattcat aacgaagggc ttcaattgga tcaagtttcg atgccttgtt
     2221 ggctggcaag actccaaaaa tcataccaac actagccgaa actgcaagac taaatagggc
     2281 aactgggatt gatactccaa cttctatacc ttctattaaa ccttgcagta acaaacctgc
     2341 taaggcagtt aaaccacttg caattgtcaa gccaattaag ccacctaaca aggtcaaaat
     2401 catggattca atcaaaaact gaattaaaat attggcacgt gttgcaccca aagctttacg
     2461 aagaccaatc tcacgagtgc gctctgtcac cgaaaccagc atgatgttca tgacaccagt
     2521 tcctccaaca aagagagaaa tccctgcgat ggaactaata atcgtcgtca taaaactaaa
     2581 cgattgttga atttctgcaa atacaacgga ctcatctgcc acctggtatt ctccctgttg
     2641 taagcctgca agctctgtca tttttcgtgc cagttctgga cccagagttg gggttaaact
     2701 ggtatcattc actcgaaaga caatactagc tatttcatct atattaaaat tcgcagcaag
     2761 ggagatattg gtagtaatag gcaagccacc aaacccatat atttttgact ttttagcctc
     2821 cggactagta taaaccccaa tgacccggta actaaatcca ttgacttcta caaccttgtt
     2881 aatagcctct tgaggagatt caaataaact aatggacaat tcctcatcta gcaaaataac
     2941 acttgcaaac tctttgaaat cttgctctct cagactacga cctgcaataa tttcattctt
     3001 aacagcgtcc atgtaagttc tgtttccacc tgtcaaatta gcattctcaa cctttttatc
     3061 ttgataggtc aagatggcat tcgttgaatt ggttacatag taactatcca ctcccttcag
     3121 tttagctgcc tcttgaaccc aggattcttg cggttttggc ggttcaacag gaacttcctc
     3181 ttcctttcca gaaaccgtaa aagctgattg tttctgagta aaagacccat ctttactttt
     3241 tttaggagag aaaaagacgc taatattttt ctgagattta gtcatatctt tattgacttg
     3301 acgagatagg gaatcaccca aagccataat cacaacaact gatgaaacac cgataataat
     3361 cccaatcata gtaagcaaag aacgcatctt gtgagccatg atagatgaaa aggcaaattt
     3421 cagattctgc atcttagttt tcctcctttc ctaactgagc actgtcagac gaaatgaccc
     3481 catcccgaat gacaatctga cgtttggcat aggcagcaat ctcaggctca tgcgttacca
     3541 tgataatggt ttttccttct ttattcaaat caaccaataa ttgcataatt tggttacctg
     3601 ttttggtatc caaggctcct gtcggttcat ccgctaggat aatagaagga ttgtttacca
     3661 aggcacgcgc aatggctaca cgttgctttt gaccaccaga taattctgaa ggtaaatggt
     3721 gactacgttc tgtcaattca accttgtcta aatattcctc agccaacttg cgacgttttg
     3781 aagacgaaac tcctgcgtaa atcaagggca attctacatt ttgcagagca ttgagcttcg
     3841 atagaagaaa gaactgctga aagacaaaac cgatttgttg gttacggacc ttagctagtt
     3901 gtttttcacc aagcccagcc acttcttgac cttcaagata atattctcca ctggttggtg
     3961 tatccaacat gccaatcgta ttcatcagag tggacttacc agacccagat ggtcccatga
     4021 tggctacaaa ttcaccctca ttcacttcta gattgatatt tttgagaacc tgcagttctt
     4081 ggtcaccatt acggtaactt ctgaagatat tttttagact aattagttgc ttcatcagcc
     4141 ttcacctctt ttccttcttc caaggaagat gttggattac tgatgacctt agcaccgttc
     4201 gttaaaccag aagtgatttc ttgattttct gcgtcagcat ttcccaatga aacctcaact
     4261 tttttagcct tttgttgttc atccacaatc cagacataat ttttactatc atccattact
     4321 agactgctaa caggaataag aatagcctta gttttgcttt taacctcaat gttgacagaa
     4381 aaaccttgtt tcaaatcacc aacctcgcct gtcacatcaa tagtataagg gtatttagaa
     4441 cctgtattat tcccggctgc tggactagct gcttcaccat tgtttttagg atagtcagaa
     4501 atatagctta atttcccagt ccatttttta tcaggataca ctttagaagt aaagcttact
     4561 tcttgaccta cagaaaggtt ggctagattg tactcagaca attctccctt gacttgtaaa
     4621 ttttcattgc tgacaatatg aaccataact tgactcgccc ctgttggaga tttagaaaca
     4681 ttgctattga cttcgactac agttccctct agggtactga gaacagttgt tgcatccaat
     4741 tgactttgag ccttgcttaa ttgcgctgca gcatctgcac gcgcatcacg ggcatcaccc
     4801 aattgagcat caatagaagc aacagaattt ccagccactg gagttgggct ttgcaccgtt
     4861 gcatcttctc ctcctactgg cgctggtaac tgtggagcct gagctgaagc ggcttcattt
     4921 cgtgcttgat tgagttcatt gatatgacga tctgccttag ctactgctcg actagctgaa
     4981 tcataggccg cctgcgcttc tgaactactg tacttgacta aagcctgccc ttcgctgacc
     5041 ttatcgccca cagaaacaag gatttcatct aaatcaccct tactagcatc aaaataaaca
     5101 tattgttcat tttttgctgt tactgtccct gacaataaaa cagaggatgc cacgcttcct
     5161 tccttggcaa caacaagatg agtaggctca tcttttacag cagtctgaga aggttgtcta
     5221 aaaagtaaaa tccccccggc acccaataca actacactcg cagcaccgat tgctgcatac
     5281 agttgccact ttttagcttt accattcttt ttcttcataa tgaaactcct tttctttttt
     5341 acaatacttt gctattatac caaatttccc tccagcaaac aatgcagttc agatggaaat
     5401 aatggcagtt caggattaaa caatcgttcg gaattttgct tttctattgt actagatata
     5461 taatacacta tcttgactta tttggcaagc ttttttgcaa ttttttgtgc gtagcagatt
     5521 tttgcaatca aatcaaaaaa agaataaaat ataactatca aaaaagacac tctactattt
     5581 aaaagattac aaaggagttt tcaatgagag aatatgatat cattgctatc ggtggaggta
     5641 gcggaggaat cgctaccatg aaccgtgctg gtgaacatgg agccaaagca gccgttattg
     5701 aggaaaagaa attaggtgga acctgtgtca acgtcggttg tgttcctaaa aaaatcatgt
     5761 ggtacggggc gcaaatcgct gagactttcc atcaatttgg agaagactac ggctttaaga
     5821 ctactgatct taactttgac tttgcaaccc tacgtcgcaa tcgtgaagcc tacattgatc
     5881 gcgctcgttc ttcttatgat ggtagtttta aacgcaacgg tgtagacttg attgaaggtc
     5941 atgctgaatt tgtagattct catactgtaa gcgtaaatgg tgaactgatt cgtgctaaac
     6001 atatcgtgat tgctacaggt gcccatccaa gtattcctaa tattcctggt gctgagctag
     6061 gtggctcttc tgatgatgta tttgcctggg aagaacttcc agagtcaatt gccattctag
     6121 gcgctggtta tatcgccgtt gaattagctg gcgtactcca cacttttggt gtcaagacag
     6181 atctctttgt tcgccgcgat cgtcctttac gtggttttga ttcctacatc gttgaaggtt
     6241 tggtcaagga aatggaaaga acaaacttac cacttcacac tcacaaagtc cctgtcaagt
     6301 tagaaaaaac tactgacggc attaccattc atttcgaaga tggtactagt cacacagcta
     6361 gccaagttat ctgggctaca ggtcgccgtc caaacgttaa gggcttgcaa cttgaaaaag
     6421 ctggagtgac tctgaacgaa cgtggcttta tccaagtgga tgaataccaa aatactgttg
     6481 ttgagggaat ctatgctcta ggtgatgtaa cgggcgagaa agaactgact ccagttgcaa
     6541 tcaaggccgg acgtacccta tctgaacgtc tctttaacgg aaaaactact gcaaaaatgg
     6601 attactcaac tattccaact gttgtctttt cacaccctgc tatcggaact gttggtttga
     6661 cagaagagca agctattaaa gaatacggtc aagaccaaat caaggtttat aaatcaagct
     6721 ttgcatctat gtactctgct tgcactcgca accgtcaaga atcccgtttc aaactcataa
     6781 cagctggttc agaagaaaaa gttgtcggac ttcatggaat tggctacggc gttgatgaaa
     6841 tgattcaggg atttgccgtt gctatcaaaa tgggagcaac caaggctgac tttgatgcaa
     6901 ctgtggcaat tcacccaact gcatctgaag aatttgtaac catgcgttaa tcgaaacaaa
     6961 agaagctgat ataaatatgt ctgcttcttt tttaatgatt ttcgaatcaa caaaaaaatt
     7021 atccaacttg actagtcacg aaaatcggct gttacagttt ttgttacctt gtttttaaaa
     7081 aatggttgac atcgactcct atatgagtat aatagttatc atacattaga aaagaggtta
     7141 actattttga aaaaagctca cgtttatgct atccctgcta ttggggctgc tctcattgct
     7201 gctttggcac aaatcagtct tccaattgga cctgttccct tcactctgca aaactttgca
     7261 atcggcttga ttgctactgt ctttagaccg agagaggctg tactttctgc tggactctat
     7321 cttcttctag gtgctatcgg tcttcctgtc tttgcaggag gtggagctgg ttttcaggct
     7381 ttagttggcc ctactgcagg ctatctttgg ttttatctcg tttactctgg acttacttcc
     7441 tctctaacca acagcaagag tggtgttgtt aagatttttc ttgcaaacct cttgggtgat
     7501 gcccttgtct ttgtcggcgg gattctcagc ttgcatttcc tagctggaat ggcatttgaa
     7561 aaagctcttg ctgtgggggt tcttcccttt atcattccag accttggcaa acttctggct
     7621 attagtttta ttagccgtcc cctacttcaa cgccttaaaa atcaggctta ctttactaac
     7681 taaaaaagga tatcgagtta tcatgactca atatcctttt cttttatttt gaaaacttat
     7741 actcaatgaa aatcaaagag caaactagaa agctagccgc aggctgctca aaacactgtt
     7801 ttgaggttgt ggatgaaact gacgaagtca gtaaccatac atacggcaag gcaaagctga
     7861 cgcggtttga agagattttc gaagagtatt agttgccttt aaaggcatcc accatcgttt
     7921 gaaattcttc atttgagaga gtaatccctt tgcccatttt agtatggtct ggactccaag
     7981 cacgaatatc aaactttgca ggggcaccat taaagctcac acggttaatt tccttggtcc
     8041 aacctttttc gttttcagaa agagtcaaca agtgctcttc gatttcaaat gtaaattctg
     8101 ccattttctt ctcctttttt agtttcatta gtttattcgt aaaatcttgt agattttagg
     8161 aaaattttat ataatattga tataaaagaa gggaggccaa tatgagacat aaattccagc
     8221 aagttctaaa taaaatacat gattttttaa atggatatga ccaacctgac cagactgaaa
     8281 ccaactccct tacagccact attgaagagg ctatccagaa acaaaccgct gttcacctta
     8341 tcttgtctga gacaagtttt acaggtgata tcatcaaata tgatcagcaa ggccagcaaa
     8401 ttatcgtgaa aaattttgcc aaaaatgtga gccggattat ccgtataagc gatattcaac
     8461 gcctgcgatt tgtcccctca actgtccaaa cagcccaaaa aaatagattt aagaaagagt
     8521 gagatgtagt tgcttcatcc cactcttttt tcttagcgaa tttgttcaaa atgtaaatga
     8581 actgcaatat gatctccata accacttctt tccaagtcac gttgtaaacg ataggaaatg
     8641 tagtgttctg caatggtaat gtaacctgcg cccaataaac gatgttcaac catagattga
     8701 atcatactga tagtcgcacg ttccaccttg gcttcttgta aatccaaaac taccttctta
     8761 gtgacttgag caagattttg acgcaaatca tctgtcaaaa cataaacagt ttgggctgcc
     8821 ttcaagatgg cttggtaaat cttatctgga ttaaattcag caatttcgcc attacgtttg
     8881 attacttgca taggtttctc ctttattctt tgttttcttt gatttctgcc agcatttttt
     8941 cttcttctac tgtcagttga taatgttcaa gtaaatccgg tctgcgctcg taggttttct
     9001 ttaaactctc gtacaatcgc cactgacgaa tcttttcatg gtggccactc atcaatacat
     9061 ctggcacgac catgcctcga taatcatagg gacgtgtgta ctgaggatat tctaaaagac
     9121 ctgaagaaaa actatcatct tggtggctag actccttgcc aatcacttct ggaatcaggc
     9181 gaactgtagc atcaatcatg gtcatagctg ccaattctcc accagtgagg acatagtcgc
     9241 ctagggaaat ctcatctgtt accaaggtct taatgcgctc atcataaccc tcatagtgcc
     9301 cacagataaa gattagctct tcctcttgag ccaaatcttc agcataagcc tgatcaaact
     9361 gctttccagc aggatcgagg agaataacgc gcggattttt cttttcaata gcatcaaagg
     9421 aatcgaaaat aggttgtgct ctgagcaaca tgccctgacc gcctccgtag ggctcatcat
     9481 ctacatgacg ggccttttca gcattttctc gaaaattatg atactggata tccaagagcc
     9541 ctttttctcg agcctttcca acgattgagt gctccagtgg agaaaacatc tctggaaaga
     9601 gggttaaaat atcaatcttc atcgtctaac ccttctaaga tttccacatc gacccgttta
     9661 tttggaatat caacattgag aaccactggt gggatataag gtaaaagcaa atcacgtttg
     9721 ccttttcgtt tgaccaccca gacatcatta gcacctggtt gcaggatttc cttgatggtt
     9781 ccaaccaagc tatcaccctc atagacttcc aaaccgataa tctcgtgata gtaaaattca
     9841 ccatcgtcta ggtcattcaa atcttcctca gcgaccttga gactatatct cttgtacttt
     9901 tcgatagtat tgatatggta catatctttg aatttaataa tgtcaaagtt cttctgttta
     9961 cggtggctag cgatggtcac tgtttggaca aactgatctt tttcatcaaa caaagccagc
    10021 tcagctcctt ttttaaaccg ttcttctgca aaatccgtca cagacaagac tcgcatctca
    10081 ccctgtaatc cctgcgtatt aacgattttc ccaacattaa agtagttcat cttgtctcct
    10141 gtaatctcct tttttccatc ttattctgac aattctcgaa taatagccgc aattttttcc
    10201 gattctgacc attgtaaata atggtgattc cctcctaaaa tgagtttagt attggaagtc
    10261 caatattctg attctctgta ctctttttct ctataaggct gacaaaaaac aaatacagga
    10321 atatgagctt ctatagatac atcctcaaaa tcttcctcag taatctctcc agatatctga
    10381 aattctggat cttgattttc caactctaag cctttttctt gcattaattc ccagattttt
    10441 ttattcgttt caggactaaa tgttgcttga gttaagttct taaaataaag ttcaggacca
    10501 cactcgtcaa tcagcctcat ctgctcttcc atttctggat aaggattttc tgaaaaatca
    10561 gcaaacatga cttttttagt tgtcggttca attgctacta aagtctgacg cttaattggt
    10621 ttctcgagta atttgcaagc taaaattcca ctccaactat gtgcacaaag tatatattca
    10681 gaaattccta attcttcaag tacttcataa accgcatctg caagattatc tagatttttt
    10741 ccagcttggt catgaatcgg actcctacct gtgttcggaa aatcaattgt caaataacca
    10801 attgtaggag gaggtttttc aagtataagt gaaaaatttt cataacttgg tagcaaacct
    10861 gctccgttta gacaaactag cactttcttt tgcttttgat aagtaacaga gaggctacca
    10921 atttctgtag atacttcaaa cctcttcata aagaaatcca ctcattctat ataatgaatt
    10981 attaaaaatc cttatctttt attttatcac gttccaagga ttttctcaag ttggaggaag
    11041 gggacaacat ctctactttc ccttcaaaaa tccttccaaa tcatgtttat gttggtaatt
    11101 aacggctgcg gttttgtctt tctcaaagac agtcttggta aggtcaatat gattaatagc
    11161 tacgattgcg acggtgtagt aaatgatatc agccagttct ctggcaagtt cctcgttcga
    11221 atcctatccc ttcttttcga ccagagcgcc tattcaaaac ctcggctact tctccgactt
    11281 cctccactaa cttcataaag agaccttcat cagtccgaga ctgctgttaa tgttcgatta
    11341 agtagtcttg gaattgccta aacgttcaat cttttatagt atattgaaac tagaatagta
    11401 cacct
//
LOCUS       DAVBGY010000058        10991 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVBGY010000058 DAVBGY010000000
VERSION     DAVBGY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10991
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.058"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(6..890)
                     /locus_tag="VQ620_001688"
     CDS             complement(6..890)
                     /locus_tag="VQ620_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HET2764278.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDMIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(894..1280)
                     /locus_tag="VQ620_001689"
     CDS             complement(894..1280)
                     /locus_tag="VQ620_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HET2764279.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLISLMLEEQILTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(1273..2616)
                     /locus_tag="VQ620_001690"
     CDS             complement(1273..2616)
                     /locus_tag="VQ620_001690"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET2764280.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYEVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEGLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(2698..3582)
                     /locus_tag="VQ620_001691"
     CDS             complement(2698..3582)
                     /locus_tag="VQ620_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2764281.1"
                     /translation="MIQVRNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHIEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(3594..4211)
                     /gene="cysE"
                     /locus_tag="VQ620_001692"
     CDS             complement(3594..4211)
                     /gene="cysE"
                     /locus_tag="VQ620_001692"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HET2764282.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFIW
                     KHGFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(4227..6440)
                     /gene="pnp"
                     /locus_tag="VQ620_001693"
     CDS             complement(4227..6440)
                     /gene="pnp"
                     /locus_tag="VQ620_001693"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET2764283.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDDQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRLELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            complement(7125..7991)
                     /gene="metF"
                     /locus_tag="VQ620_001694"
     CDS             complement(7125..7991)
                     /gene="metF"
                     /locus_tag="VQ620_001694"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET2764284.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(8055..10304)
                     /gene="metE"
                     /locus_tag="VQ620_001695"
     CDS             complement(8055..10304)
                     /gene="metE"
                     /locus_tag="VQ620_001695"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET2764285.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVASDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(10565..10831)
                     /locus_tag="VQ620_001696"
     CDS             complement(10565..10831)
                     /locus_tag="VQ620_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2764286.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGVSQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
BASE COUNT         3069 a         2512 c         2182 g         3228 t
ORIGIN      
        1 cttctttaca aaccaactat agttgacaaa gaacctaaaa tcaattgata acacaaggtc
       61 aggtcggtca actctttcaa ctgaagccct gtcaactctt cccatttatc aatcttgtat
      121 tggagagaat tgcggtgcag atagagttgc tgggctgttt tagtgagaac agcactattt
      181 tcccaaagag agagaatgat ttcctgaatc tgatcttgat ccaaaatcat ctggtgtaga
      241 cattctttaa ttggcttcaa gtccacgagt ctttctccca tactccaaag atagagctga
      301 gaaaaagtat gaacaccttg gtgaccctga cgccaccatg tcttgaacaa atcccgctca
      361 gctttgatta agtctgatag ggcttgatgt cctgtctgag accaaacctg acccaacatg
      421 atagaaagac gaagtccaaa gtcatactca actgcttcaa tcatatcact taaaatatct
      481 cttacagaag tgtatttgtc ttgttgaagc acgaaaacat aatcctgagc tccgacctgt
      541 aacactgtct gacaattcgg aaaaagagtc cgcatcatat ctagccaaga agccagattt
      601 tcctgctgaa aataagaaag atggcaataa accaactgaa tctttttaaa aacttgcggt
      661 gcctgtccct tgccttcaac cagataggaa taccaagggt ttagcgaacg agcctgctcc
      721 tgctgggtca aaagggcaac caactgcttt tcacgctcgc tgagcccagc ttcctccagc
      781 aaaatccact gctgagaagc taaaggaagc gtgagatagc cctctttctc tactggttgg
      841 tctgaaatcc gagcctcagg aaaccagtct tgtagttctt ttgccctcat gttctagccc
      901 tccacttttt ggatgcacca tgaaaccaaa ctctcaagac gttccagatt ctcagtcata
      961 tggagatagc ccataactgc ttcaaatccc gtggacatac gataagtcac gacatctgca
     1021 tttttagcct ttgtgtgact attggtattg cggccacgtt tgtagatttc ttcttctttt
     1081 tccgttagga tctgctcctc caacatgaga gaaatcaggc gagcttgagc cttggctgac
     1141 acgtacttgg tcgcctcttg gtggagttta ttgggcttgg tcataccttt gaggatgagg
     1201 tggcggcgaa tatacataga atacaccgca tctccctcaa aggctagcgc aatcccgtta
     1261 atgagattga catcaatcac gtgtccacct cactccatcc ttggtatcaa ggagcttaat
     1321 tccttgagta accaattggt cacggatttg gtctgctgtc gcaaagtcac gattggcacg
     1381 cgcctcttgg cgtttttgaa tcaagtcttc aatctctgca tccaaaactt cctcaacaaa
     1441 gacaattcca aaaatttcta acatatctgc aagagcttgc ttgacacttg catcatagtt
     1501 ccctgagttg atccatttgg ccatttcaaa gacaactgtg ataccgttgg cagcattaaa
     1561 atcttcatcc atagctgcta caaacttatc tttaaagttt tgtaactctt gggcatccac
     1621 gtttcctgta aatggttgtt cgtaagtatt cttcagatac ttgagattgg tctcggcatc
     1681 gcgaactgcc ttttccgtga agttgatagg cttacggtag tgctgggtcg caaagaagaa
     1741 acgaagtact tgcccatcaa gagttttaag ggcatcgtgt accgtaatga agttacccaa
     1801 ggacttagac attttgacat tgtcgatatt gacaaagcca ttgtgcatcc agtagttagc
     1861 aaaagccttg cctgttttag cttcagattg ggcaatttca ttggtgtggt gtggaaactc
     1921 taggtcagct ccaccaccgt gaatatcaat ggtatcacct aaaatctctg tcgacatgac
     1981 tgaacactca atatgccaac ccggacgtcc aggtccccaa ggactatccc aagaaatctc
     2041 acctggtttg gaagatttcc atagagcaaa gtctacagga ttttccttac gagccgtttc
     2101 ttcatcggta cgacctgaag cacctagctc caaatcttcc aaggttttat tagccaattt
     2161 agcatagttg tgggattttt ctacacggaa atagacatcc ccttgactct cataggcaaa
     2221 gcctttctcg atcaagcctt ccacaaaacg gatgatgtct gccataaact ccactacacg
     2281 cggatggcga gtcgcaggtt tcacgcccaa ggccgtcaca tcctcacgaa aggcagcgat
     2341 gtacttatcc gcaacctcct gaggcgtgat gccttcttcc ctggcacggt tgataatctt
     2401 atcatccaca tccgtaaaat tggaaatata ggcaacctcg tacccacgat actcaaaata
     2461 gcgacgaatg gtatcaaaag ctaccgttga acgagcattc cctacatgaa tatagttgta
     2521 caccgttggc ccacaaacat acatcttgat cttgccgtcc tcaatcggga caaattctcg
     2581 caaatcacga gacatggtgt catagatttt aatcataaat cataatcaag aaagctgaaa
     2641 tccaagaaca attagtttca tcactaaaag ttcaagtaaa tttcagtccg aatatctcta
     2701 cacttcggaa tcccttgctc ctttctcatt cagataaacc acctgagtct gtttgacaaa
     2761 gccaattttt tcatacaaac gtttggcacc tacattgcta tcttccactg caatctgaaa
     2821 ttccttgtca ttttgctcaa ttagttggtt gacgagggat tttgctaagt agcttccata
     2881 gcctttccca cgttcaggtt ccgatattgc taaaccgtag aagtaattcg tattagtcga
     2941 taaatctacc gtgcaagtcc caataacctg accgtctttt aacaaaatat atagtcggct
     3001 ttctggatcc ttcagagctt cagcgacata tctatccaca acttctctcg attcatgttc
     3061 ctctgaaaat gcctgaaatt ttaattgact aatttgatcc tgatacgaac tatctgctaa
     3121 caaaacttca atatgggaaa catttgctaa cggataaggt cttctatcct tacctaacca
     3181 agtttctgtc tcttcatcct cgattagtcc ccagttactg gcaaagtcag gatgattctc
     3241 taaaaaaata cgttctgtct gaaaagtgac tgatcgaatg gggaaagaag ctgtttctct
     3301 ctcaaaacta gtaaacaatg cacgcgcaat cccctgacgg cgatgacctg gatgaaccag
     3361 tatcgtcact tctacatctt ggtcatctgc atagacagtt aataaaccaa caagttcgcc
     3421 tttttcataa taaaggaaaa aggcgggcat gtttgggtca aaattaagca tgttagagag
     3481 atagggatcg cgataggtac cgtcatagtt ttggcaacag ttaattactt ttttcgcctc
     3541 agatagctcc tcttggctta acttgtttct tacttgaatc atataggtat cctctacaaa
     3601 ccagacgatc tgtgactggc atctttagcc tgctcgagtt tattgacata atactctcgt
     3661 ttttcttcga cttcgtgaat gacaggctca tctttcttac catgaagacg gacaatcttg
     3721 gccggaatac cgacaaccgt cacatcacta ggtacatctg ctacgacaac tgctgcagca
     3781 ccgaccttgg cattttcacc aatttccaca ggtccgataa cttgggcatg ggctgatatg
     3841 agggctccct ttcgtacagt cggatggcgt ttgccacagt ctttccctgt tcccccaaga
     3901 gtcactccgt gatagagaag aacgcctttt tcaacaatcg ctgtctctcc aatcaccaga
     3961 ccagaaccat ggtcaataaa gacacctgaa tcaatctggg ctcctggatg aatctcaatc
     4021 tgagtccaaa agcgccaaaa ctgactgtgc atacgagcta agagtttgaa gccgtgcttc
     4081 cagataaaat gcgagagacg gtgggccgcc aaggccttga cacctggata agtcagcaaa
     4141 acctccaaag tggtgcgggc cgctggatca ttttctttta cgatatcaat ggtttcgcgc
     4201 caccacccca tacatttctc cttttcttat tctgaatctt ttggtgtttc tgtaaattct
     4261 ttcttaggtt tgtgatcctt gtgatgacgt gggcggtgag gacgctcaga cttttcacct
     4321 ttttcatcat gctcaggttt tggcggacga ggtagaagag ccttcataga ggcatcgata
     4381 cggccttttt catcaatctt gataacctta acatcaactt catccccgat ttctaccaaa
     4441 tcctctacac gattggtacg agtccaagcc atctcagaga tatgaacaag ggcatctgtc
     4501 ttatcaaaga ggttaacaaa ggcaccaaat ttctcgatac gaacgacttt agcacggtaa
     4561 acttcatcca ctttggcttc acgaaccaaa ccagcaataa tttctttggc acggttaata
     4621 gcatcttggt cactagagta gatagacaca tttccttctt cgtctatatc aatcttaaca
     4681 cctgtttcag cgataatctt gtcgatggtt tctccaccct taccgatgac aatcttaatc
     4741 ttgtccacat caatcttgat cgtatcaatt ttcggagcag ttggagccaa ttctagacga
     4801 acttctggaa tggttgcttc aatgacatca aggatttcaa aacgcgcttt cttggcttga
     4861 gcaagagcct ccgtcaagat ttctgcagta atcccttgaa tcttgatatc catttgaagg
     4921 gctgtaatcc catcacgagt acctgcaacc ttgaagtcca tatctccaaa gtgatcttcc
     4981 aaaccttgga tatctgtcaa tactgtgtag ttatttccat ctgagataag tcccatagca
     5041 ataccagcta ctggcgcctt gattggcaca ccaccagcca taagggcaag agttcccgca
     5101 cagatagaag cttgagatga agaaccgttt gattccaaaa cttctgctac tagacggata
     5161 gcgtatggga attcttccaa gcttggcaag acttgagcaa gagcacgctc accaagggca
     5221 ccgtgaccga tttcacgacg acctggcgca ccgtaacgac ctgtttcccc tacagaatat
     5281 tgagggaagt tatagtggtg cataaagcgt ttcttgtact ctggatccaa accatcaatg
     5341 atttgagttt ctcccatcgg agccaaggtc aagactgaaa gagcttgagt ttgcccacga
     5401 gtaaagagac ctgaaccatg tacacgagga aggaagtcaa caaccgcatc caaaggacgg
     5461 atttcatcga ccttacgacc atcaggacgc accttgtctt ctgtaattaa acgtcgcact
     5521 tctgcgtgtt ccatttgttc caagatttca gccacatcac gcataatacg gtcaaattct
     5581 tcgtggtccg catatttttc ttcgtaaacg gcagtcactt ggtctttcac tgcttgagtt
     5641 gcagcttcac gggccaattt ctcttctact tgaactgcct tttggaggtc actgttgtag
     5701 gctgcaatga tttcagcttg caattcagca tccacgtgaa gcaattccac ttctgctttt
     5761 tctttaccga cagcagcaac gatttcttct tggaaggcaa tcaattcttt gacagcttcg
     5821 tgccctttaa gaagcgcttc caacatgatt tcttctgaca attctttggc accagactct
     5881 accatgttga tagcgtgctt agttccagct actgtcaatt caagaagaga ttgctctgct
     5941 tgttcttgac ttgggttgat gatgatttgg tcatctacat atcccacttg taccccagca
     6001 attggtccgt caaatggaat atctgaaata gacagtgcca aagatgaacc aaacatagca
     6061 gccattggtg cagatgcatt ttcatcataa gaaagcactg tattgatgac ttggacttca
     6121 ttacggaaac cttccgcaaa cataggacga atcggacggt caatcaaacg cgctgtcaag
     6181 gtcgcatctg ttgaaggacg tccttcacgt ttcataaagc caccaggaaa cttcccagcc
     6241 gcatacattt tttcttcgta gttgacttgg agtgggaaaa aatccccagt tgccattttc
     6301 ttagacataa cggcagcagt caagacagtt gactcaccgt aacgtacgac aacagagcca
     6361 tttgcttgct tagcaacctg accagtctct acaattaact cacgacccgc aaaagtcgtt
     6421 tgaaacactt gttttgccat tttaatcccc tttggattga tgaaattata cgccttgcct
     6481 acaaagatca agataccaag gacgtcaaaa gcaaagtaaa aataggaaac tgacgaagtc
     6541 ttcgatgaag acaagacagt ttatcttttt tacacagctt ttcggccgtg ttcaattaca
     6601 caagatattt tggacggttc ggcttgccga acatttctgt agaaaaatag gaaggtgacg
     6661 tcgcactcga cgagtgctag gaagcttatc ttttttccta agaaatgaga ccaaaattca
     6721 agtcatcaag ataccaagcc gtcaagcaac tcaaaggaaa ataggaaatc gaacgacgga
     6781 gcgactgctc ctaggcagat ttatcttttt ccacagagtt gtaggcgtgt tcagttttca
     6841 agatacatca ttagaaaggt ttaatattaa agtatctaaa cctttcacgc taatcgctat
     6901 cgggcgatta gctaaatgct ttactaactc tttcgtcaaa taacatcgat ttgactcact
     6961 cgtgtcgtta aatcttacag tttaaatgca ttgtattatt taataccttc atctttgtat
     7021 caagtacgta cagaatctat tttatcatat ttttcttaaa aagtgaagtc tttaccatta
     7081 aaaaggaacc attcccctca cctgagaaga atggtttgct tttattatcc tagagactgg
     7141 tgattaaaca aggcatgggt tgcttgatgg atgtattttg ctgtatcagc attattcatc
     7201 gtatagagat gcacaccggc aacatcctga gttaccaagt ccacgatttg gtccactgca
     7261 taggcaagtc ctgctgctct gagcgactca gggtcatgct catacttgtc taagatggct
     7321 ttaaatttgc gtggaagatg gatattctca caagtcttca agagtcggag agcctgattt
     7381 cgattcagaa ttggcataat tcctgcatga atgggaacat caatcccagc caagatacac
     7441 ttgtcctgaa aatcatagaa gcgctcattg tcaaagaaga gctgagttac gaggctcgaa
     7501 cagcctgcat ccactttctt cttaagattt tgaatatctg aaatctgatt tggcgaatct
     7561 ggatgccctt ctggatagca agctccaata atatcaaagt gaggggcttg ttccttgata
     7621 aactcaatca agtcggttgc atagcggaaa tccttttgtg gttctacgtc tggaataata
     7681 tccccacgaa gagccaagat tttctgaacc ccaactttgt ccaagtcagc aatagtttca
     7741 gcaaccttgt ccttagttag ataaatagct ggcaagtggg caatggtcgg aatcgccaag
     7801 tcattttgga taaagtcagc caaacgaacc gtcgtttcct tgatattaaa tttattattg
     7861 ctggcagtta cactgataaa atggggagcc aactcctgca tatcctgcaa ggctgaaata
     7921 atgttaccat tacccacggc tgggtttgga gggaacactt caaatgagag tgacggtgtt
     7981 tggcgtgaca tatgtaataa ccttttctag ttgatttctt tttgaacaac cactgtatgg
     8041 agagaaatcc aatcttacaa tttctcacgc gcagctttag ctgcttcaac aaggcggatc
     8101 aagctttctt ttgtttctgg gataccacgt gttttcaaac cacagtcagg gttgatccaa
     8161 actttcttgc ttggcacttt agcaaggatg gcttcgattg tgttgtcgat ttcgccttca
     8221 tttggtacac gaggtgagtg gatatcgtaa accccaggtc ccacttctgt ttggaagttt
     8281 ttcgctttga gttcgtccaa gatttcaagg tttgaacggc tggcttcaaa ggaaataacg
     8341 tctgcatcca tgttatcgat agctgggatg atatctgtaa attctgagta acacatgtga
     8401 gtgtggattt gtgtgtctga cgctactgtt gagtgtacca agcggaaggc aggaattgcc
     8461 cagtcaaggt aatcttcgta ccagtcacta cgacggagtg gcaatttttc acgaagagca
     8521 gcctcatcaa tttggatgat tttcacacca gcagcttcaa ggtcaagcac ttcatccttg
     8581 atagcaaggg cgatttgaag agtagaatcc ttgatagaga tgtcttcacg tgggaatgac
     8641 cagttaagga tggtaacagg tccagtcaac atacctttaa caggtttgtt tgtacgactt
     8701 tgtgcatagc tagaccattt aacagtgata gggttaagac gagtgacatc accccagatg
     8761 attggtggtt ttaccccacg cataccgtat gattgtaccc atccattttt agagaagagg
     8821 tatcctgaca agttttgacc gaagtactca accatgtcat tacgctcaaa ttcaccgtga
     8881 acaaggacat caaagtcaat atcttcttgc cacttgatcc attcgtcaat cgtttcagca
     8941 aggaaagcat cgtactcttt ttgagacaat tcacctttac ggtaagccaa acgtttggca
     9001 cgaacttctt ttgtttgagg gaatgaacca atcgttgttg ttggaagagc tggaagtttg
     9061 aaagcttctt cttggatagc ttcacgttct gcaaaggctg gcaaacgagt gtagtctgcg
     9121 tctgtcaagc cagcgatacg cgcacgaagt tcagcatttt caccaacacg ctcagtcgca
     9181 aagagttctt tgttggctgc aagagcttct gaaccttgac cattgcggat agcatccaag
     9241 tcacggattt catccaattt ttcaactgca aaggcaaagt ggttcaagag tgctggttca
     9301 aattcttcat tagcagttgt aaatggcaca tgaagaagtg agcaagagct tgtcaaaacg
     9361 atgttttcag ctggaatttg ctcaagaaca gccaagctct tttcgtagtt gttgcgccag
     9421 atgtttttac cattgacaat acctacatag agagtcttgt cagctgggaa gccaccttta
     9481 acgagttcaa gagttttctt accttcaaca aagtcaagac cgatagcatc tactggtaag
     9541 tttacaaggt cagcgtatac gtcacgaaca tcaccgaaat aagtttgaag caagacttca
     9601 agaccttttt tgtcagccaa gagtttgttg taaaggttca agaagagagc tttttcttca
     9661 gctgtcaagt cttttacaag agccgcttca tccaattgga tgcgagtcgc accaagttca
     9721 gccaatttag caaaaacttc ttggtaagca gccactaagc tatctacgaa gtcgtctgct
     9781 ttcacgcctt cttcaaagtc tgacaattga aggaaagtga agggacctac aagaacagga
     9841 cgagtgttca atccaagttc tttggcttct tggaactcat cgaaaatctt gtgaccagcc
     9901 aattttactt gagtgtcttt ttcaaattta ggaacgatgt agtggtagtt agtgttgaac
     9961 catttcttca ttggaagggc gcgaacgtcc cctttttctc cctggtaacc acgtcccaaa
    10021 gcgaagtagc gctcaaggtc agacaagtcc aagttttgaa cggatgcagg caccacgttg
    10081 aaaaggaaag ccgcatctag gaagttatca tagtgagaaa agtcatttga tggaatttca
    10141 gtgatgcctt tttctttgac aatgttccag tgtttagcac gcaagtcttt tgctgctgct
    10201 aaaagttctt cttctgagat ttcttttcta aagtattttt cagttgtaaa ttttaattca
    10261 cggaattcgc ccaaacgagg gaaaccgatg attgtagttg acatgatgtg tcctccaaaa
    10321 tttgttgttg aaactatctt aacagaaaag aaagcgtctg tataattgta aaaaattagg
    10381 gtttgatata gtttgaaact atatatctgt ttcggacaaa agaaaaagac ttgaagcaaa
    10441 cgtctcaaat cctttgtaat tcttacttta cagctatatt ccaattagaa tactaaaaca
    10501 tgttattagt aattcttata agtgactatg acctgttatt agaaaagact ataactgatt
    10561 ctagtcaact tttcccctgt tcaagtggaa cgactgctag tgtctttcct agactggtta
    10621 gaacttttaa gaccgtatcc aactgaagac tgctctctcc tgtttccatc ctagctatga
    10681 caggttgact cactccactg acttcttcca gctttttctg actgattcct tgttcatacc
    10741 tagcctcaat caactcgctc atgatagcca ctcgcatatc actttcaagg atttcctcct
    10801 tgctaaagag ctcagatgga catccttcca attactccca atagcactat tcttcatcac
    10861 ttaaccctct ttcttttacg tctatgtatt tttaaaagaa tgagcgaatt atgattcgat
    10921 agattgacca gcaggtttaa agttggtgct agcctgtttc ttaagcgctt ttccttttct
    10981 agggtaaagc a
//
LOCUS       DAVBGY010000059        10732 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVBGY010000059 DAVBGY010000000
VERSION     DAVBGY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10732
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.059"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            51..233
                     /locus_tag="VQ620_001697"
     CDS             51..233
                     /locus_tag="VQ620_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET2764287.1"
                     /translation="MKSQKIKVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTIL
                     RLESASIVSELVAGLR"
     gene            533..1372
                     /locus_tag="VQ620_001698"
     CDS             533..1372
                     /locus_tag="VQ620_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HET2764288.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            1390..3228
                     /locus_tag="VQ620_001699"
     CDS             1390..3228
                     /locus_tag="VQ620_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2764289.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPDVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTPAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     NLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            3241..4656
                     /locus_tag="VQ620_001700"
     CDS             3241..4656
                     /locus_tag="VQ620_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET2764290.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSAIPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            5248..6294
                     /gene="pheS"
                     /locus_tag="VQ620_001701"
     CDS             5248..6294
                     /gene="pheS"
                     /locus_tag="VQ620_001701"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET2764291.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            6294..6803
                     /locus_tag="VQ620_001702"
     CDS             6294..6803
                     /locus_tag="VQ620_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2764292.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            6880..9285
                     /locus_tag="VQ620_001703"
     CDS             6880..9285
                     /locus_tag="VQ620_001703"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET2764293.1"
                     /translation="MLVSYKWLKELVGIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEILSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKKFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYDKAVNFKEFTLTETNEAATDALSVGIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SHVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYVLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLEVTYTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSVIEAALQPAVPFVEITKFPAVSRDVALLLKAAVTHQEVIDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEKKVNA
                     EVR"
     gene            complement(9458..10459)
                     /locus_tag="VQ620_001704"
     CDS             complement(9458..10459)
                     /locus_tag="VQ620_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET2764294.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIADDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            10615..>10732
                     /locus_tag="VQ620_001705"
     CDS             10615..>10732
                     /locus_tag="VQ620_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2764295.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNREIASLLGK"
BASE COUNT         3439 a         1723 c         2283 g         3287 t
ORIGIN      
        1 accccaaaag ttagattttt tctgtctaac ttttggggtg tagttcacag ttgaagagcc
       61 agaaaattaa agtgtctttt actcaatcaa aaatagtaac gcctattctt ttggtgaata
      121 agactattgt ttggtatggt gcaatgccat tattagggaa ggtagatgag atgaccatat
      181 tacgtttgga atcagctagt atagtttctg aactagtggc aggtttacga tagagaaaat
      241 ttttaaaaat ttctatgtat gattttcatt tctttagtga gactgttgcc attatcacat
      301 tcgaatcaca caaaataaaa aaatttctac aagtacttga caaatagatt gaaatatcat
      361 aaaatgaaaa tggttacaga gttattaatt atttaagctt catgtcacca ttaaaaattg
      421 aaataaaagg atgttatcac taatacaagt gagcaggaac ctatttaatc acatcggaag
      481 aagtttcttg atgtttttaa gtaggttcct tttattttaa aagggaaatt ttatgatcat
      541 aaaacgaata ctaaaccaca atgccgtaat tgcgcaaagt aaaaaagata tcgatattct
      601 tctttttgga aggggcatag cttttggaag aaaaactgga gataaagtaa atccaattga
      661 tattgagaaa agtttttttc tcaaaaatag agataatatg acccgtttta cagagatgtt
      721 tattaacgtt cctttggagt tggtgtacat caccgaaaaa ataattaacc taggtaaaat
      781 aacattgggt aataattttg atgaaattat ctatattaat ttaacggatc atatttcttc
      841 gagcatagaa cgttataaag aagggattat tatttcgaat cccctacgct gggaaatatc
      901 gaaatattat aaagaagaat ttgaacttgg gaaaagggct ttacaaataa taaaaaaaga
      961 gttaggtatt gaacttccaa ttgacgaagc tgcattcata gcgctacatt ttgttaatgc
     1021 taatttagaa aataattttc aagagtcgta taaaatcact gaaataatta tgggaattga
     1081 gaaaatcatt caagatttct attgtactga gtttaaccaa gattctattg attattatag
     1141 attcataact catataaaat tatttgctca tcgcttggtt gaaaatacaa cttattgtga
     1201 cgatgatgat gaagacttgt tagcattaat gaaaaataaa tatcctagag aatatgaatg
     1261 tggtgaacag gtggcaatgt ttatacaaac tgagtacaat tatttgctga cttctagtga
     1321 attagtttat ttgatggctc atattcgtcg cttaacaaaa aatttagatt aatagaatag
     1381 gagaatacca tgagttataa agatacggta caaaaaatcc tcgatgtaat tggaggtgaa
     1441 aaaaatgtca atagagttac ccattgtgta acacgtttaa gattagaatt aaaagatgaa
     1501 aatttagtca atgatgatga tgtgaagaag ataccagatg taataggtat tatgaaaaag
     1561 aatggacaat atcaaattat acttggtaat gatgtagcta attattataa agaattcgtt
     1621 aaacttggca attttgaatc cgattcagtt gttcaagggc acaaagggaa tattttagaa
     1681 agaatcattg agtatatcgc tggttccatg actccaatca ttccagcaat gttaggggga
     1741 ggtatgttga aagtcttggt aatcatttta ccaatgcttg gtatattgca atcagattct
     1801 cagactattg cttttttgac attttttggg gatgctccat attatttctt accgctgtta
     1861 ttagcttatt ctgcatcaca aaaattaaaa gtaacatcta cattagctat gtctgtagca
     1921 ggtgtacttc tccatccaaa ttttgttcaa atggtgcaat cagggaatcc tcttagttta
     1981 tttggtgcac ctgtgacacc agctagttat ggttcatcag tcgttccaat tcttattatg
     2041 gtttggttga tgaaatatat tgaaaaaata attgctaaat taacaccagc tattactaag
     2101 agttttttgc aacctacgct agtattatta gtatcaagct gtattgcctt agttgtagtc
     2161 ggacctattg gagtaattgt tggtgaagga ttatcaaatc tagttgggca aatgtatggt
     2221 gtagctggat ggcttacatt agctattctt ggtgctatta tgccatttat tgttatgact
     2281 ggaatgcatt gggcttttgc acctattttt ttggcggcat ctattgctac tccagacgta
     2341 ttaattcttc cagcaatgtt agggtcaaac ttagctcaag gggctgcttc gatggctgtt
     2401 gcattaaaga gtaaaaataa taatacaaaa caaattgctt ttgcagcagg tttctcagcc
     2461 ttacttgcag ggattaccga acctgcatta tatggtgtga ctttaaaata taaaaaaccg
     2521 ctttatgcag ctatgattgg tggtggatta gcgggattat ttgcaggtct tactagtgtt
     2581 aaagcatatc tatttgctgt cccatctttg atagcgttgc ctcaatttat ttattctgat
     2641 gtgccatcaa atattgtaaa tgctttaatt gtggcggtca tttcggttgt tattaccttt
     2701 gtattagctt atatatttgg aatcgatgaa gaagagagtt ctagcaattt agaagttaaa
     2761 gctggagttt caaataaaaa aatgatattt tctcctatat caggagaaat cattccgtta
     2821 agcgatgtcc aggataaaac attttcagat aaattaattg gagacggagt agcgattatc
     2881 ccaagtgaag gtaaggttta tgcaccattt gatgggaaaa ttacaaatat ttttccgact
     2941 aagcacgcaa ttggattgaa gagtgatgag ggtgttgagt tactaattca tattggatta
     3001 gatactgttg agctaaaagg tcaaggtttt attagtcatg tagaagaagg agacagagtt
     3061 ttcaaaaatc agttgatttt tgaaatgaac ttgaatttaa tcaagactaa aggctacgaa
     3121 acagttacac cagtaattgt aacgaatacc aatgattttc tagatgtatt agtattacct
     3181 aataatcaga caatcgagca ttctaaggaa ttactggtaa tattataatg gaggaaagtt
     3241 atgaccattt ttccagatga ttttctttgg ggtggagcgg ttgcagctaa tcaagtagag
     3301 ggagcatata atgaagatgg taagggctta tcagttcagg atgtgttacc caaaggtgga
     3361 ttaggagaag caacagaaaa tcctacagaa gataacttaa aattgatagg tattgatttt
     3421 tatcataaat ataaggaaga tatatccttg ttttctgaaa tgggctttaa tgtttttcgt
     3481 acttctattg catggagtag aatttttcca aaaggagatg aggaagaacc taacgaagct
     3541 ggtttgaaat attatgatga attatttgat gaactacatg ctcacgggat agaaccactt
     3601 gtaactcttt cacactatga gactccatta tatttagcaa gaaaatatca tggatggatt
     3661 gataggagaa tgattcattt ttatgaaaaa tttgctcgaa cagttttaga aaggtataaa
     3721 gataaagtta aatattggct tacatttaac gaagtaaact ctgttttgga attaccgttt
     3781 actagtggag gaatagatat acctaaggag aatctttcga aacaagaatt atatcaagct
     3841 atacatcatg aattagtcgc ctcgagtttg gttacaaaaa ttgctcgtga gattaattca
     3901 gagtttaagg tggggtgtat ggtattagct atgccagctt atccaatgac tccaaatcca
     3961 aaagatgtat gggcgactca tgagtatgag aatctaaatt atctattttc agatgtgcat
     4021 gttagaggtt attatccgaa ttatgcaaaa agatatttta aggaaaatga cattaacata
     4081 gagtttgcag ctgaagatgc agagttatta aaaaattata ctgtagattt tttatccttt
     4141 agctattaca tgagtgtgac tcaatctgct attccaacac agtataattc aggagaaggg
     4201 aatattattg gtggtttagt aaatccttat ttagagtctt cagagtgggg atggcaaatt
     4261 gatccaattg gactacgtat aattttaaat agatattatg accgttatca aatcccatta
     4321 tttatagtag aaaatggatt aggtgctaaa gatcaactaa taaaagatga acttaataac
     4381 ttaaccgtcc aagatgatta tagaattcag tatatgaaag aacatttatt gcaagttgct
     4441 gaagctctac aggatggtgt tgaaattatg ggctatacgt cttggggatg tattgactgt
     4501 gtttctatgt ccactgcaca actttctaaa aggtacggtc tcatttatgt tgatcgaaat
     4561 gatgatggaa atggcacatt caatcgatat aagaaaatgt cctttacttg gtataaagga
     4621 gtgattgaat caaatggaga atccttattc aaatagtttg tttattgatg attagatatt
     4681 atcacggtta aggcaacatc tcattaaata taaaataaag aggtttggaa gacatcaaat
     4741 tctaaacctc tttattttat agggggatta attaatttaa ttttgttaca gagagccgat
     4801 tataaatagt taacttagga tagattggtt atatatagta gactgaatct aaaatagtac
     4861 gaaacaattg ctaaaacatt tatagaaatt aattttactt tcccaatcga tttgttttca
     4921 tcttatttca atccgctata aagttagata agaataagct aacaagcaga agtaagatag
     4981 gtcttgcaag aatggtaaaa atctgataaa ctaagtagta attgaatagg aatctgaaag
     5041 actagtagcc caagggaagt atatcaggga ggagagccgt gactgcaagc tctctatatg
     5101 aaagctgggt gaattcactt gcgcatggat ttagaaatga aggtctgact tgtcagatga
     5161 aaacggatgg taccgcgtgt caacgctccg agtggagttt ttggcatgtg gttttctttt
     5221 tatctacgag agactgatgg aggaaatatg tcaactattg aagaacaatt aaaagcgctt
     5281 cgcgaagaaa cgctggctag cttgaagcag attactgctg gaaatgaaaa agagatgcaa
     5341 gatttgcgtg tctctgtcct tggtaaaaag ggttcgctca ctgaaatcct caaagggatg
     5401 aaagatgttt ctgctgaaat gcgtccaatc atcgggaaac acgtcaatga agctcgtgat
     5461 gtcttgactg ctgcttttga agaaacagct aagctcttgg aagaaaagaa agtcgcggct
     5521 caactggcta gcgagagtat cgatgtgacg cttccaggtc gtccagttgc gactggtcac
     5581 cgtcacgttt tgacacaaac cagtgaagaa atcgaagata tcttcatcgg tatgggttat
     5641 caagtcgtgg atggttttga agtggagcaa gactactata actttgaacg tatgaacctt
     5701 ccaaaagacc acccagctcg tgatatgcag gatactttct atatcactga agaaatcttg
     5761 ctccgtaccc acacgtctcc agttcaggcg cgtgctatgg atgcccatga tttttctaaa
     5821 ggtcctttga agatgatctc gccagggcgt gtcttccgtc gcgatacgga cgatgcgacc
     5881 cacagtcacc aattccacca aatcgaaggc ttggtagttg ggaaaaatat ctctatggct
     5941 gatcttcaag gaacgcttca gttgattgtc caaaaaatgt ttggtgaaga gcgtcaaatt
     6001 cgtttgcgtc catcttactt cccattcaca gagccatctg ttgaggtgga tgtttcttgc
     6061 ttcaagtgtg gtggagaagg ctgtaatgta tgtaagaaaa caggttggat cgaaattatg
     6121 ggggccggta tggttcaccc acgtgtcctt gaaatgagtg gtatcgatgc gactgtatac
     6181 tctggctttg cctttggtct tggacaagag cgtgtagcta tgctccgtta tggaatcaac
     6241 gatatccgtg gattctacca aggagatgtc cgcttctcag aacagtttaa ataatgatta
     6301 gaaaagtaga aatggcagat gttgaggtgt tggctaaaat tgccaaacaa gcctttcgtg
     6361 aaacctttgc gtatgataat acggaagagc agttacagga atactttgaa gaggcttata
     6421 gtctgaaaac tttgtcaact gagttgggaa atcctgactc tgaaacctat ttcattatgc
     6481 atgaggagga gatagctggt tttctcaaag tcaactgggg aagtgctcaa actgagagag
     6541 aattagagga cgcttttgaa attcaacgcc tctatgtgct acaaaaattc caaggatttg
     6601 gactaggtaa gcaactgttt gaattcgcac ttgaacttgc tacaaaaaat agtttttctt
     6661 gggcttggct aggtgtttgg gagcataata caaaagctca agcgttttat aaccgatatg
     6721 gttttgaaaa atttagccaa catcatttta tggttggtca aaaagtagat acggattggt
     6781 tactgagaaa gaaattaagg taagaagaga attcttctat tgaaatgata ggaaaggaaa
     6841 agaaccagtg tggcaatggt cattctggag aactaaatta tgcttgtatc ttataaatgg
     6901 ttaaaagaat tggtgggcat tgatgtgcca tcacaggagt tggctgaaaa aatgtcaacc
     6961 acagggatcg aggtcgaagg tgtcgaatca ccagctgccg gtctttcaaa aattgtcgtc
     7021 ggtgagatct tgtcttgcga agatgtgcca gaaactcacc ttcatgtttg tcaggttaac
     7081 gttggcgaag aagaagagcg tcagatcgtt tgtggtgccc caaatgtgcg tgcaggcatc
     7141 aaggttatgg tggctcttcc gggagctcgt atcgctgaca attacaaaat caaaaaagga
     7201 aaaatccgtg gcttagagtc acttggaatg atctgttcac ttggtgaatt gggtatttct
     7261 gactcagttg tacctaagaa attcgcagat ggtattcaaa ttttgccaga aaatgccgtt
     7321 ccaggtgagg aagtcttttc atatctagac ttggatgatg aaatcatcga actttctatc
     7381 acaccaaacc gtgcagatgc cctttctatg cgtggagtgg ctcacgaagt ggcagccata
     7441 tatgacaagg cagtcaactt taaagaattt actttgacag aaacaaatga agctgcgaca
     7501 gatgcccttt ctgtgggcat tgagacagac aaggcgcctt actatgcagc tcgtatcttg
     7561 gacaatgtga ctatcgcacc aagtccacaa tggttgcaaa accttctcat gaacgaaggc
     7621 atccgtccca tcaataacgt tgtagacgtg acaaactaca tcctgctcta ctttggtcaa
     7681 cctatgcatg cttttgactt ggacacattt gaagggactg acatccgtgt gcgtgaagcg
     7741 cgtgctggtg aaaaattggt gaccttggac ggtgaagaac gtgacttgga cgtgaatgac
     7801 ctagtcatca ctgttgcaga caagccagta gcccttgcag gtgtcatggg tggtcaagca
     7861 acagaaatct ctgaaaaatc tagtcatgtt gtccttgaag ctgctgtttt caatggcaaa
     7921 tctatccgta agacaagtgg tcgcctgaac cttcgttctg agtcatcttc tcgctttgaa
     7981 aaaggaatta atgtggcaac agttaatgaa gcccttgatg cggcagctag cctgattgcg
     8041 gaacttgcag gtgcgacggt gcgtaagggt atcgtttcag cgggtgagct tgatacttca
     8101 gatgtagaag tttcttcaac tcttgctgat gttaaccgtg tcctcggaac tgagctgtct
     8161 tatgctgatg tcgaagacgt cttccgtcgt cttggctttg gtctttctgg aaatgcagac
     8221 agctttacag tcagcgtacc acgtcgtcgt tgggatatca caatcgaagc tgatctcttt
     8281 gaagaaatcg ctcgtatcta tggatatgac cgcttgccaa ccagccttcc aaaagacgat
     8341 ggtacagctg gtgaattgac tgtgacacaa aaactccgcc gtcaagttcg taccattgct
     8401 gaaggagcag gtttgacaga aatcatcacc tatgttctaa caactcctga aaaagcagtt
     8461 gagtttacgg ctcaaccaag taaccttacg gaactcatgt ggccaatgac tgtggatcgt
     8521 tcagtcctcc gtcaaaatat gatttcaggt atccttgata ccgttgccta caacgtggct
     8581 cgtaagaata aaaacttggc cctttacgag attggaaaag tctttgaaca aacaggtaat
     8641 ccaaaagaag aacttccaaa tgaaatcaac agttttgcct ttgccttgac aggcttggtt
     8701 gctgaaaaag atttccaaac agcagcagtt ccagttgatt tcttctatgc taaaggaatc
     8761 ctagaagcgc tctttactcg cttgggacta gaagtgacct atacagcaac agctgagatt
     8821 gccagccttc acccaggacg tacagcagtc atttcactcg gtgaccaagt tcttggtttt
     8881 cttggccaag tgcatccagt cactgctaag gcttacgata ttccagaaac gtatgtggct
     8941 gagctcaacc tttcagtcat cgaagctgcc cttcaacctg ctgttccatt tgtggaaatc
     9001 acgaaattcc cagcagtcag ccgtgacgtt gcccttctcc tcaaggcagc agtaactcac
     9061 caagaagtta tagatgctat ccaagctgcc ggcgtgaaac gtttgacaga tatcaaactc
     9121 tttgacgttt tctcaggtga gaaattggga cttggtatga agtcaatggc ttatagcttg
     9181 accttccaaa atccagaaga cagcttaacg gacgaagaag tcgcacgcta catggaaaaa
     9241 atccaagcat cgcttgaaaa aaaagtgaac gcagaagtgc gttaaacgat agtaggatac
     9301 aaattccccc tctagtttat gaacactaga ggggtgattt tatataatac tcaatgaaaa
     9361 tcaaaaagca aactaggaag ctagccgtaa gctgtacttg agtactgtaa ggcgaggctg
     9421 acgtggtttg aagagattgt cgaagagtat aaaataatta aaatgtgatt tctgtaaaaa
     9481 tacatctatg atcagagttt tgaaaactta caatgtctaa atctttaaca tagtagtggt
     9541 ttttaggcaa taaaatatga tctattgttg cattaaaaag ttttggactt tggctattcc
     9601 aagttcctct ttcaaaaggt ggtagtacat ttaatgcgtc cctatgagag cttatttttg
     9661 caagtgctcc atgacgcata gttgcattaa aatcatctgc aataatagcc tttggatatt
     9721 ttgaagccaa ttgattatga atgatgttta agtcttgctt ccagatttcc attaaacctg
     9781 gcagaggagg agcagtatgc aaggcaatga tatctggtat attttgtttt ctcgaatgta
     9841 atacaattgt cccgaaccgt gttgtatgaa aagttttagc ttctgtatag aaaccataac
     9901 ttttcttgac aatcacagtc acaggagcta ttccactatt ggtaggtgga gaagtgaaaa
     9961 tatcatagtt ggccatagaa agtccaactt gatgaaacaa tagtttgatt ctctggtttt
    10021 cttgctcacc tctgatattg gtagctagtt cagggaatat agccatatcg gcgtcaaaat
    10081 ggctaaaaat tcgctcaata tgttgtgctt ctatttgatt agcgacgttc caagttacta
    10141 attttaaggt tttagtctta tttgatgcag aagaaagggt tttatctgtt ccgaataata
    10201 gtaatatgag cgatagtaag agcaaaatgc cagagtaaaa ccgtatttgt ttatttttct
    10261 tgtaaaaaga tagggaaaaa aatacgaaac ttaaaatagc agccatagtt gcagtagcta
    10321 aggggaaggc ataaatctgt gccaagatag gtctggtaga tagccaaata ttaagattag
    10381 aagatggaaa aaaagaataa gttatgatag ggataacaaa gagaatgacc gaaagggcag
    10441 agagaaatgt tttaatcatt gtgtgagtac ctcaatttgc aatggttgct cggagctaat
    10501 gtgactttgt gactttactt gattgatatt ctttttcttg tcttcctcat agattaagta
    10561 caacccaagt aaaaaaagtc caatttaaga ttgaaatgat taaaaggcac agaaatgcaa
    10621 gacaattata ctacaaaagg caaacatttg acaatcgata gccgtcgctt aatcgaaaga
    10681 tggaaaaaag aagggaaatc aaatagagaa attgcctctc tgcttggaaa ag
//
LOCUS       DAVBGY010000060        10643 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVBGY010000060 DAVBGY010000000
VERSION     DAVBGY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10643
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.060"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..96
                     /locus_tag="VQ620_001706"
     CDS             <1..96
                     /locus_tag="VQ620_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2764296.1"
                     /translation="IVDYIDYYNNKRIKVKLKGLSLVQYRTKSFG"
     gene            complement(212..1015)
                     /locus_tag="VQ620_001707"
     CDS             complement(212..1015)
                     /locus_tag="VQ620_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HET2764297.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1296..2051)
                     /locus_tag="VQ620_001708"
     CDS             complement(1296..2051)
                     /locus_tag="VQ620_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HET2764298.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(2121..4772)
                     /locus_tag="VQ620_001709"
     CDS             complement(2121..4772)
                     /locus_tag="VQ620_001709"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET2764299.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYSTTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPKFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTDDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNEAGEIYVGEEAPAGDGWKQDEDVLDTWFSS
                     ALWPFSTMGWPNTETTDFKRYYPNSTLVTGYDIIPFWVSRMIFQGIEFTGKSPFKNAL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIDKYGTDSLRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAEYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(4765..5343)
                     /locus_tag="VQ620_001710"
     CDS             complement(4765..5343)
                     /locus_tag="VQ620_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET2764300.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYQLMYLKQIQNLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(5343..5813)
                     /locus_tag="VQ620_001711"
     CDS             complement(5343..5813)
                     /locus_tag="VQ620_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2764301.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGYG
                     IVVKGTDKIVGSVDFNHRHEDDVLEIGYTLHPDYWGRGYVPEAARALIDLAFKDLGLH
                     KIELTCFGYNLQSQRVAEKLGFTLEARIRDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            complement(5800..6378)
                     /locus_tag="VQ620_001712"
     CDS             complement(5800..6378)
                     /locus_tag="VQ620_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET2764302.1"
                     /translation="MKQSFKTNKLYYGFPVFILGYQDQNFGYNITTCSSSYSLGDWVV
                     IGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEKLERLGVHYEISERTQ
                     TPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDEKGHFKNQLFAPTYFM
                     GDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(6661..7056)
                     /locus_tag="VQ620_001713"
     CDS             complement(6661..7056)
                     /locus_tag="VQ620_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET2764303.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(7152..7406)
                     /locus_tag="VQ620_001714"
     CDS             complement(7152..7406)
                     /locus_tag="VQ620_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HET2764304.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            complement(7415..8749)
                     /locus_tag="VQ620_001715"
     CDS             complement(7415..8749)
                     /locus_tag="VQ620_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HET2764305.1"
                     /translation="MTDERIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVLDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTVKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKRLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(8749..8982)
                     /locus_tag="VQ620_001716"
     CDS             complement(8749..8982)
                     /locus_tag="VQ620_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HET2764306.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLTGTPMDKIVRTLEANGYEVIGLD"
     gene            9013..9087
                     /locus_tag="VQ620_001717"
                     /pseudo
     CDS             9013..9087
                     /locus_tag="VQ620_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            9076..9201
                     /locus_tag="VQ620_001718"
     CDS             9076..9201
                     /locus_tag="VQ620_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
                     /protein_id="HET2764307.1"
                     /translation="MDRTDEVSNHTYSKVKLTWFEEIFEEYKMILLLILYHMERD"
     gene            complement(9251..9391)
                     /locus_tag="VQ620_001719"
     CDS             complement(9251..9391)
                     /locus_tag="VQ620_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764308.1"
                     /translation="MKFFWTILAILFIKPIIGIVKFFWMIISFAVQLLFYKILDWFFK
                     LI"
     gene            complement(9388..9570)
                     /locus_tag="VQ620_001720"
     CDS             complement(9388..9570)
                     /locus_tag="VQ620_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764309.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKDDHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(9572..10355)
                     /locus_tag="VQ620_001721"
                     /pseudo
     CDS             complement(9572..10355)
                     /locus_tag="VQ620_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3097 a         2353 c         1959 g         3234 t
ORIGIN      
        1 attgtagact atattgatta ctacaacaat aaacgaatta aggtaaaact aaaaggactt
       61 agccttgtgc aatacagaac taaatccttc ggataaatta tttgtctaac tttttggggt
      121 cagtacacct aaaactttga tgatatacgt tttcttgtga gaatatttac ttcatttttg
      181 cctaaaattc aatgtttact cagtatttgg attatgaaaa atcgaggtct aaatctagat
      241 acattttttc tgaagacaaa tcattttgac caccgagcaa gagattttca aaaaaagctg
      301 ttaaaaactc agaacgtcgc tgtaaaatct ttgcattatc taataccaag gcatcacgaa
      361 aatatttgga atgttgctga aatggtgtat tatcaatatc aaaaccaaac tcacgaagat
      421 actgaatcaa aaagaccgtt actgtccgag tgtttccttc gcgaaatgga tgaatctgcc
      481 agattcctga aataaaatgc tggatttgtt taaccacatc cgcctgagtt agtgtcgcat
      541 atgcaacttg tttttcctga ttaaaatcat aatctaaggt catttgaatc atggagtaat
      601 cagagtacac aacactttca ccattcaaaa caggttcatt ctttgtgata ttggtctgac
      661 gaaattgccc caccggaata gagggttcaa atatatcttg aaacaactcc ttatgaatag
      721 caagtaaggt cgcaggacta aagctaaagc ctcttcgaga caatagttct acaatacgta
      781 gagaaaccaa gtctgcctct tccgtacttg catcaatagt atggtgataa gccgttgcat
      841 cctcataaac ctgctcataa gtcagttctc cccgggactg tttctcagcc aaagattcca
      901 tatacgctga tggcactaga ttgtcaactt tctgcagacc aaaacctatc cgccataaat
      961 cacgcttcgc ttcataagac aagtttggat tgtcaatgtt gtaagttgat tgcatagaaa
     1021 tatcctttca attgtttttc ttcattacca gtaaatcacg aaattggttg gaaactggct
     1081 taaactataa aaagtcctca caataggttc tatgatatat atatatatat acttcatgta
     1141 aataataaaa gagttataat cagcactact gataaattca ataccgattt cgccatctta
     1201 aaacttagtc aaacgaatac cgttgatttc cagttcatta agtcgttgac ctgtcaagac
     1261 ttctcgttcc ttcaactgga aaagttaagg gggaactaat acaaatcctc tacgcttcta
     1321 tattcaatga tttcattttt gataatattc tttttaaact caaaatgttt taaaggatta
     1381 tcgattaaca ctaactttgg aatatcatct agatttgtga ccaaggataa aataaaatcc
     1441 gattgaaact ccttgacttt ttcaaattct tttgcagtta aacgcacacg ccccttagat
     1501 tttcggatac cttttacttc ggcaaggtaa gaatactctt ggacatctac ctgaaagtca
     1561 tatccatcac cataaagacg ggcatcggtt aactgtccac cttggaattt ttcttcttta
     1621 tcaaaatgaa ggataaaata attctcagcc tccattcccg tttcctgtag acgtttgaat
     1681 ttagttcgaa ctattggttt ttcaacgatg gttatgcctg ttttcttccc ttcactggca
     1741 agtaacattt taactatttc agcataacta tgaacatctt catttccaaa catcatatca
     1801 attaaatctt tacgaaaacg ataaacttca gctttctgcc accaaccttt tcgattattg
     1861 tcaaaataag gatcaaataa atccattcta ttttttacaa ctccagttgt ttcagcaatt
     1921 cccagagaaa ccacatagcg ataaaactcc gacttgctag agcattgaaa ttcttttata
     1981 aaaagcttgt caaacttggc aagaccgtag ccaattaaat ttaacaattc ataatgaggg
     2041 tgtttggaca tcactttctc catcaataga tattgatttc attataccaa aaaacacggc
     2101 tcatcaccgt gtttctgtgt ttatttcacc aacttcttca tctcatcaat acgtgctacg
     2161 gtcgcatcat acttagcttg gtaatctgct tgcttgtcgc gttctttttg gacaacttct
     2221 ggtttagcgt tggcgacgaa gcgttcgttg ctgagttttt tgccaaccat gtcgagttct
     2281 ttctgccatt tggcaagttc tttttccaga cgtgccaatt cttcttcgac attcaagagg
     2341 tctaccagtg gcaagaagat ttcggctcct gtgatgacgc ttgacatagc cagttcaggt
     2401 gcagggatgg ttgatgcgat ttccaagtgt tcaggatttg tgaaacgttt gatgtagttg
     2461 acattgctgt taaagaaggc ttccaagtcg ctatcgcttg tcttaacaag gatagtgata
     2521 ggcttgcttg gagcaacgtt tacttccgca cgcgcattac gaacggcacg gatcaagtct
     2581 ttaaggcttt ccacaccagt gtgagcagca aggtcttcaa aggctgggtt gacagttggg
     2641 tattctgctg taacgataga tccttctgag atttgtccaa agatttcctc tgtcacaaat
     2701 ggcatgattg ggtgaaggag acgaaggatc ttgtccaaag tgtatagtag aacagaacgt
     2761 gtgatgactt tctcttcttc gttatcgcta taaaggactt ccttggtcaa ctcaacatac
     2821 cagttggcaa attcttccca gatgaagttg taaaggatat gaccagccac accaaactcg
     2881 aacttatcaa agttttcagt aacttttgca atggtttcgt tgagattgtg gagaatccag
     2941 cggtccgtca cattaccagc ctcacctgtt gcaacttttg tgacattgtc atgcgccaca
     3001 tccagcgtca aaccttcatt gttcatgagg atatagcgag aaatgttcca aattttgtta
     3061 ataaagttcc atgaagcatc cattttctcg taagagaaac gaacgtcttg accaggagct
     3121 gagccgtttg acaagaacca acgaagacta tctgtaccat atttatcaat aacatccatt
     3181 gggtcaatgc catttccaag agatttagac atcttacgtc cttcttcatc acgaattaga
     3241 ccatggataa gagcattttt aaatgggctc tttcctgtaa attcaattcc ttggaaaatc
     3301 atgcgtgata cccagaaagg aataatatca tagcctgtta cgagtgttga atttggatag
     3361 tagcgtttaa agtcagtagt ttcggtatta ggccagccca tggttgagaa gggccagagg
     3421 gcagagctga accaagtgtc aaggacatct tcgtcctgtt tccagccatc gcccgctggg
     3481 gcttcctcgc cgacatagat ttcgcctgct tcattatacc aagcagggat ttggtgaccc
     3541 caccagagct gacgggaaat cacccagtcg tggacatttt ccatccattg caggaaggtg
     3601 tcgttgaagc gcggtgggta aaattccacc ttgtcatctg tgtcttggtt ggcaatggcg
     3661 ttcttggcca attggtccat cttgacgaac cattgagtag acaagcgtgg ctcaactacg
     3721 acacctgtcc gttctgagtg accgactgaa tggacacgtt tttcgatttt gacaagggca
     3781 ccgatttctt ccaacttagc aacgactgcc ttacgagctt caaaacgatc catgcctgaa
     3841 aattcaaagg caagctcgtt catagttccg tcgtcgttca tgacgttgac ttgtggcaag
     3901 ttatgacgtt ggccaaccaa gaagtcattt ggatcgtggg caggtgtgat tttcacgaca
     3961 ccagtaccaa acttaggatc tgcgtgctca tcaccaacga ttgggattaa tttattagca
     4021 attggaagga tgacattttt accaatcaag tccttgtagc gcgggtcttc tgggttgacc
     4081 gcaaccgcaa cgtccccaaa catggtctca ggacgagttg tagcaacttc aagggcgcgt
     4141 gaaccatctt ccagcatgta attcatgtga tagaaggcac cttcgacgtc cttgtgaata
     4201 acctcgatat cagaaagggc tgtgcgagct gctgggtccc agttgatgat aaactcacca
     4261 cggtagatcc agcctttctt gtaaaggtcc acaaagacct tacgaacagc ttttgacaaa
     4321 ccttcgtcaa gagtgaaacg ctcacgagag tagtctacag agagccccat cttgccccat
     4381 tgttccttga tagtagtgga atattcgtct ttccattccc agactttcgt caagaaagac
     4441 tcacgaccaa ggtcatagcg ggaaatgccc tcaccacgca agcgctcctc taccttagcc
     4501 tgagtggcaa tccctgcgtg gtccatccca ggaagccaaa gggtatcaaa accttgcatg
     4561 cgtttttgac ggatgataat atcttgcaaa gttgtatccc aagcgtgacc aaggtgaagt
     4621 ttacctgtaa cgtttggtgg tggaataacg attgaataag gcttagcctt ttgatcgcct
     4681 gaaggcttga aaacatcagc atcaagccat ttttggtaac gaccagcctc aacctcggct
     4741 ggattgtatt tagatgaaag ttctttagac atgtgtgtgt cctttctcta ttttgtttat
     4801 tttattttga atttgcttag cagcttcttc tgcagacaaa ttcgtattat ttattttaaa
     4861 gtagtggtgc aactcattcg gttgatattg ggaatttaat tgaagtgttt cagcggtctc
     4921 taaaatttct ctttcagata cctcaatatg tcgttttaag ggtttgtgct ttaatcgatt
     4981 ctccgttcga tttcgacgta tgcactcttc aagacttgtt tccaactcaa caaaaagaat
     5041 ctcttgatga taatcatcca acaaattctg aatttgcttt aaatacatca gttgatactg
     5101 atttgaaaaa tcaattacat ctgttaaaat tactgatcgc tgatttcttg cacttgctcc
     5161 aaggaatgaa aaagtgattc cacgaacaaa ttcccacatt tcctctgtat agtcctgata
     5221 gatttctagt gcaaaatcaa tggcttgatg gttataaaat agggtagcat ccgtcagtcg
     5281 agataattct tgaccaatgg tcatttttcc tgatgctgga gcaccaatga tgaaaagatg
     5341 catcaaatca cctcccactc actcttcagc aaggcatatc tcaaatcatc acagcagttg
     5401 ccttgagcat ctttacggtc tcttatgcga gcttcgaggg taaagccaag cttttccgcg
     5461 actcgttgac tttgaaggtt atatccaaag caagttagtt caatcttgtg aagacccaaa
     5521 tctttaaagg ctaagtcaat caaggcacgc gcagcttctg gcacataacc tcgcccccaa
     5581 tagtctgggt gtaaggtata gccgatttcc agcacatcat cttcatggcg atggttgaaa
     5641 tcgacagagc caacgatctt atccgttcct ttgacgacaa tcccgtagcc tgctggatag
     5701 gcgacctctg gcaaactagc atagtcaaag atatcctcag catccgccac agtacggact
     5761 cgtaaaacga gacgttctgt ttcgatttta tctggcagct cagttcttgc catccttctt
     5821 cctcgctttt ttgatgaaac tgcccttcat atctacacgc ttatccagat agcgataaac
     5881 gcgctgatat ccatctccca tgaaataggt tggggcaaac agttgatttt taaaatgtcc
     5941 cttttcatcc aggagttctg gggcaacaag tcgctcaaga atcttggcaa agatgtggca
     6001 aataccgtct tcctcaacaa tcctatctac ccgacaatct aaaacaagtg gacaggcgtc
     6061 taaaatagga gtctgagttc gttcagaaat ttcataatgc actcccaaac gttccaattt
     6121 ctcctgatga ctgataaaac cagcctgctc catcgcaagc atagaagttt catcagaaat
     6181 attcacagta aatttttgat actgtttgat ctgctctgcg gcattctctc tcgcaacgac
     6241 tccaatcaca acccaatctc ctagactata cgaggaacta caggtcgtga tgttatagcc
     6301 aaaattctga tcttgatatc ctaaaataaa aacaggaaaa ccataatata gtttattagt
     6361 tttaaatgat tgttttataa caacctcctt tgactaagac acaaaagaaa agccctgcca
     6421 tctacatgac agggacgaat gtatttatcc gcggtaccac ccaatttcgg gccaagcccg
     6481 catctttagt ctttaaaata aaaagacaga aatatttcat tttttcagtc attagcaacc
     6541 agttttgaca catttgtgga cttctcagcg ccgccacttt ctgtaaaatg tgggattcaa
     6601 aacacctcta atagctctat tgtagcaagt ttttctcgga aatgcaaggc tcaagaaaaa
     6661 ttacttgaac ttgatgccat tttctttcaa tttcttgatg gtagctgggc cgattccttt
     6721 caaggcaagg agttcttttt cagtccagtt tttgaaatct gaagcagact tgattccttc
     6781 atcatagaaa gtcttggcac gatcaagtgg aagtccaccc aagtttgctg caaaatcttc
     6841 tacagaatta gctacttttt gagcttgctc tttggtagct tctactgctt gttcaacttt
     6901 tttagaaaca gccttaccag ctgcgcttgc tgactgttct gcacgcttca cgactttttt
     6961 tgtctcatct acaaccttag tcacagtact tgaaaaggca cctgcgcgac ggaggccatt
     7021 tcgtaattgt tttttacgat tgagtttctt tgacatgata aaatcctttc aataaaaaat
     7081 ccctgctctg acaaggattt aacataaaca ttctatcaag attttagtga aaaatcaaga
     7141 atctttctcg tttaataatt tcgctcacca aacaagcctt ctggcaaatc atggaatccc
     7201 ttgcctactt tgaagttttc aaacttacca agcaagaact gataagcatc caagcgactc
     7261 tcgtagcgaa tcatggcatc tttggcacgc tcgtcctggt cttcttcgta aactttttga
     7321 ctttccttgt gcgccatgtc gttaatcatg atttgggtat tgacccaagc ttcaaattcc
     7381 ttcataaatt cttgttcgta actcattttt ctccttattc taatccacga aaatagtccg
     7441 tatcaatctc acggtagggc tggatatcct gaacatactc caacactcct tggaattctc
     7501 cgtcttcatc gtgtactgca gcataggtga tgtggacaaa cttacctcgc gactcagact
     7561 tgaaccacat ttcatacttg tctttgcttc cctcacgaag cctcttcatg atagttttga
     7621 ccttgtccaa gtacttaggc ggatggcaga gttcgacatt gcgcccgact tgagacggcg
     7681 tccgtttgaa aatcatctca tcagctggcg tattgtcatt gtaatactgg aaaatatctt
     7741 ctttattgac aaaggtaatc tccataggga gatgattgag gatgagattg gcctgctcga
     7801 ctgaaagata gccattacca aaagcctgtt gactatggcg gtccagcaca gcttcctttt
     7861 ccttaggggt aaaggtaatg gtaaaatggc cttctggcgt atctatgact tgttgaactt
     7921 gaccttctgc cgtatctagc tgtacaggct cctctgcaat cttttcctca ataaagctct
     7981 gtcgttctgg cacccatttc tctgacggac ggatgatggc atagccatag gcatcgctct
     8041 cctccgcaat ctgaagccag tcatcctgag taaaagactc aaggagaatc atgaggagga
     8101 tggactcttc cttgaaaatc atactttcaa actctgtcgc aaaagcttca aaagcttcct
     8161 ttacactgct aattgacact tctggtagtg acttgactgt cgttagagct gtttgaaaga
     8221 gttccctaat ctgatcatcc actccccaca taactttggg aggtgaatcg tgtccatagc
     8281 gctccatgat aggaaagaag agttcttcct tacgttggta atggatgtca aattgcccca
     8341 caagccccat ctgacgcacc aagcccttgc gcatctccgc aagcatttcc tcgtcttcca
     8401 tcaactcata ggtatctaac aatctacgaa tgcgaatcaa agctgcacgg agggccaaat
     8461 tttcttcctt aaagacacga actgggtgcc cagggtgttc ggtatctaaa acttcgacac
     8521 ctttgatagc atttttaaaa agattggcat ggacatcaca gagttccata acatcttcaa
     8581 aagtgacgcc cgaatccgag ttcatcagct cgtgctccat aagggaaatc tcgatggctg
     8641 acacgcccgt aaaggtcgca tcaaagcgat cttgaaccga ctcaggagag gcgccattgt
     8701 gcaattctaa caaaatatcc cgtaggatat gaatccgttc atctgtcatt agtctaatcc
     8761 aatcacttcg tagccattcg cttccagtgt gcgtacaatc ttgtccatag gagttcctgt
     8821 tagcttagaa ccctgtttaa gtgatacttt acgaccaact gtattgcgca ttaagggatt
     8881 ggcaaggggt ttaaaaccca actccactag aatttccaag acttctggat gcttgtccac
     8941 cacttctgca acaggaattg acacatcgat gatattgtcc atgacgacct ccattgtatg
     9001 ttctaatact caatgaaaat caaagagcaa actaggaagc tagccgcagg ttgctcaaaa
     9061 cagtgttttg aggttgtgga tagaactgac gaagtcagta accataccta cagcaaggtg
     9121 aagctgacgt ggtttgaaga gattttcgaa gagtataaaa tgattttact gcttatatta
     9181 taccatatgg agagagatta aaaaactagc agtggagttc ctgctagttt tctgcgactt
     9241 ggattaccat ctagataagt ttaaagaacc aatccaatat cttgtaaaac agcaattgaa
     9301 ctgcaaaaga aatgatcatc caaaagaatt tcacaatccc aataatcggt ttgataaaaa
     9361 gaatagcaag aattgtccag aaaaatttca ttctctctcc tctggcttaa tgagccaccg
     9421 agccgtatcc attagcttat ctgcaataaa gagttttccc agaccaacaa cgacatggtc
     9481 atctttcttt atcgttttca tcacttttac accttgcacg gtcaaaaagt aattaccata
     9541 aattttagca aaagctttga taggattcat attattctcc tttgatgatt tcagcctctt
     9601 ttcggattgt ttcaacatct tcttgatatt gaacttcact atagttgact agcttggata
     9661 attctttctg caagctttcc cccattggtt tcatagttgc aaaggttaaa ccacctgaaa
     9721 tgataccacc caagatagga ataaatttcc ccattccttt ggccaaccct cccttggtaa
     9781 gattcacacc aaatattttt aagacttttt tcaaaatagg gtaccaaagc gtctttgtta
     9841 aagctttatt aggcactgtt ttcattacct gtttggcaat tattatacca ccagcacgta
     9901 gcaaacaaag cagcggttcc attcacccct aacattacgc ctagataaag caagagggta
     9961 ttttgagcat cttcactcaa ctcctcacgt gaagcccaaa gatcctcata accataaata
    10021 taacctaatt cttgggccaa tttcagagag aaaccataaa attgagccac ataagctgga
    10081 atggtaattg ccatagcaag ccctccaggt aatcctgcca aaacagaagt cccactcgct
    10141 aataaaacat tatccctaat acaggcatta gccactcgat ctaagatttc ttgagataag
    10201 agagacgttg gaccttgttc caataaggta ggaatgtcct gtggacccaa ttctttggaa
    10261 aacttatcca ctaaaaattt cgaacgatca accttaacaa ctggtagttt taccacttgt
    10321 tgtaatactt gcatagctaa gtcttgttca gccatataca aactccttat ttcttttttg
    10381 caccttatca tattatattt cctacaaaaa aagcattttc taattagata acattaaaaa
    10441 atacagaacc ccttttttat agtaaaataa aacaagaaca ggacaaatcg atcaggacag
    10501 tcaaatcgat ttctaacaat gttttagaag tagaggcgta ctattctagt ttcaatatat
    10561 tttaaaagaa tatttttcca tttcaaattc aacctatttt cctccaccaa aaaagggctc
    10621 tataatattt gtagtgggta aat
//
LOCUS       DAVBGY010000061        10463 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVBGY010000061 DAVBGY010000000
VERSION     DAVBGY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10463
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.061"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(15..539)
                     /locus_tag="VQ620_001722"
     CDS             complement(15..539)
                     /locus_tag="VQ620_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HET2764310.1"
                     /translation="MKIAIIGYSGSGKSTIAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAVDCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(592..828)
                     /locus_tag="VQ620_001723"
     CDS             complement(592..828)
                     /locus_tag="VQ620_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET2764311.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLVK"
     gene            complement(896..1816)
                     /locus_tag="VQ620_001724"
     CDS             complement(896..1816)
                     /locus_tag="VQ620_001724"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HET2764312.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            2006..2415
                     /locus_tag="VQ620_001725"
                     /pseudo
     CDS             2006..2415
                     /locus_tag="VQ620_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2644..3225
                     /locus_tag="VQ620_001726"
     CDS             2644..3225
                     /locus_tag="VQ620_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2764313.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            3222..4499
                     /locus_tag="VQ620_001727"
     CDS             3222..4499
                     /locus_tag="VQ620_001727"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HET2764314.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            4517..5179
                     /gene="deoC"
                     /locus_tag="VQ620_001728"
     CDS             4517..5179
                     /gene="deoC"
                     /locus_tag="VQ620_001728"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET2764315.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGDLKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            5166..5555
                     /locus_tag="VQ620_001729"
     CDS             5166..5555
                     /locus_tag="VQ620_001729"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2764316.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGNVYTGVNIE
                     NVSYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            5646..6698
                     /locus_tag="VQ620_001730"
     CDS             5646..6698
                     /locus_tag="VQ620_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HET2764317.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDIANKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            6842..8377
                     /locus_tag="VQ620_001731"
     CDS             6842..8377
                     /locus_tag="VQ620_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2764318.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            8370..9428
                     /locus_tag="VQ620_001732"
     CDS             8370..9428
                     /locus_tag="VQ620_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2764319.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILVTIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            9431..10387
                     /locus_tag="VQ620_001733"
     CDS             9431..10387
                     /locus_tag="VQ620_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2764320.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         2986 a         1857 c         2390 g         3230 t
ORIGIN      
        1 aaaaaggaag ccctttatga attgtaggac ttccttttct tatccagaaa ttgatctagc
       61 tctctctgat ttcgaagaat agtgacttta tgtgaatatt cttggcaaag tttttggtaa
      121 ttttcttttt gagttttgct acgcccatcc caaagaatcc atctgataaa ctcccactca
      181 aagcgttcag ggcaatctac cgccatactt tctctgactt ttccacggta tttaagataa
      241 cgcttaaagg ctctaaagag acaggtcaat ggcgaaaaat tgagaaagat gatttggtca
      301 gcttcttgca ttcgttcttg gtagtagcac caagaataat taccatcgat gacccaagct
      361 ttatgcttgg tgagaaagtt ttttatctcg gttaacatcc attcgcagtc actgtcttgc
      421 caaccaggtt gaaattggag tgtgtccatg tgcagttttg gaatggagta gtagttagat
      481 aacttttctg ctatagttga cttaccagaa ccagaatatc cgataattgc gattttcatt
      541 ttctaccttt tcctatttgg agacaaaaaa acagcctcta tggactgttt cttatttaac
      601 aagtttagct gaaagacgag ctttatcgcg gcttgctttg tttttgtgaa tcaaaccttt
      661 agtttctgct ttatcgatag ctgagctagc agcacggaaa agttcttcag atgggtttgc
      721 ttcgaaagct tttatagcag tacgcatagc tgatttttga gctgagttct tttcgttttg
      781 tttaacgttc aattcagcgc gtttgatagc tgatttaatg tttgccaatg ttcttacctc
      841 catatttact aactatacca ttatatatga aaacttacgt tttgacaagg gaaaattact
      901 tttttaagta aatttcatcg atttcatggt tcttgctttt atgaagaatc acttctgcac
      961 gatttctggt tggttcaata taattttgca gatttgtgag attgatactg atccagacct
     1021 gatgggcaaa ggactccact tccccaatcg gcatctgagt aaaacgataa tagtagctat
     1081 cagggtcgtt ttgggctaga ctcagcattt tcaagaaacg gtccagatac caactttcaa
     1141 tatcatccac tccagcatct acatagatgg aaaagtcaaa gaagtcagtg atatagagac
     1201 gatcgttttg tggattttga aagacattaa ttccctcaac aattacaaaa tcagcagctt
     1261 tgacactttg tttctcttcg ggtacgatgt cgtaaacttc atgagaatag acaggaatat
     1321 ctacatcttg tccatttttg atgcggtcca agaagttgag aagagcttcc atatcatagc
     1381 tttcaggaaa tcctttacga tttaaaatcc cctgctcaat caaggtttga ttgggataga
     1441 gaaaaccatc agttgtaacc aactcaaccg tagcatctgt aaacgtacgg gacagtagga
     1501 tttgaagtag gcgactggtt gtggattttc caacggcaac actcccagaa accccaataa
     1561 taaaaggttg agatttactt tcacgttgga ggaaaattcc ttttgaaaag gctaaatctt
     1621 ccttagttcg cttgtaaatc tgaatcaaat gagccaaggg gagatagata tctgtaacgt
     1681 cttggagact gatttggtca ttaaaactct tgatagattc caattcttct tctgtcaaag
     1741 gaggtgttgt ctttcgatgt aaagattgcc aagtctggcg gctgattttt tcaaaatgta
     1801 aaaattcgtt ggtcatttgt tttcccctag atattttgtc tatcatacca taaaaagcta
     1861 aaaaaataaa gcggtttctt gaatgtggta agtcaatggt atatagtata agtgatcaat
     1921 ttattgatga aataggagat agattctact gatttataag cttataaagg gggcttttta
     1981 gttcacaact tataggaggt gtactatgaa aatcttaaaa cgttacatat tggaactctg
     2041 ttttatttta agttttgctt taccttttat aaaaggaacc aatgcagata atggtagatg
     2101 ctttgtggaa acctattacg gttttacttt tttgatggaa catgctattg taacagctgt
     2161 ctttatttgt tcgttcttaa ttgctttctt actaaaaaac gatggacgaa atggattgct
     2221 gcgggtagtt attgcttttt agtcctacgg attgcgacag agggttattt cttccacatg
     2281 tcactagaag atttgatacg actttggaca agtttggaaa tcctgacaca aacgtatcag
     2341 ttgggctttt atctaaacat tttgttagga attttgttga taataaagta ttttgttgat
     2401 aataaagtat tttaaggtta agcaatagaa ataataatct gcctgattat agatgataaa
     2461 ctttttgttt ttatctggac tgtgatattt atagttctaa atggtttatt atttataagt
     2521 gttgaaagag attgaagaaa atggcttcgt gacttgcttt cttcggtctt tttcgtttta
     2581 ttatcaattt gtaaacgatt tacaatttga gtcaaattat ggtaaaatag tgtcatgagt
     2641 aaaatgtact atgcagaaaa tcctgacgct gctcacgaca ttcatgagtt gagagtggac
     2701 ttgttgggag aaaaaatgac ttttttgaca gatgcgggtg tttttagcaa gaaaatggtt
     2761 gactttggaa gtcaactctt gctcaagtgt ctggatgtca accaaggaga gacagtccta
     2821 gatgtaggct gtggttatgg tccattaggt ttgtccttgg tcaaggttta cggagttcag
     2881 gcgaccatgg ttgatattaa cactcgtgcc ctggatttag ctcagagaaa tgctgaaaaa
     2941 aataatgcaa aagcgacgat atttcaatct aatatttatg caagagttca agggcgattt
     3001 gaccatgtca tttccaatcc tcctatccgt gcgggcaagc aagtcgttca tgaaatcatc
     3061 gagaaaagta aagatttctt ggaaattggt ggagatttga ccatcgtcat ccagaaaaaa
     3121 caaggagctc caagtgccaa gtctaagatg gaagatgtgt ttggcaattg tgaaatcctt
     3181 aaaaaagaca agggatacta tatccttaga agtgtgaaag aatgagagca gttgatttaa
     3241 tccaaaaaaa acgagacggt caagaactga cttcaagtga aattgaatgg ctgatagaag
     3301 gctatgtatc aggaactgtt cctgattatc agatgtctgc ctttgctatg gctgtttatt
     3361 ttaaaggaat gacgactcga gaaatctctg atttaacgat gaatatggtt aagactgggc
     3421 aagagtttga cctgtcagcc attgatggtg tgaaagttga caagcattct actggtggtg
     3481 tcggcgataa ggtaaccttg attttagctc ctcttgttgc tagcttcggt gtgcctgtag
     3541 caaaaatgag tggtcgtggt cttggccata caggcggaac aattgataaa ttggagtcca
     3601 ttaagggcta tcaagtagaa cgtagtcaag aggattttat tcgtcaggtt caggatattg
     3661 gagtatctgt cattgggcaa tcagaccagc tggtcaaagc agataagctt ctctatgccc
     3721 ttcgtgatgt gaccgcaact gtcgacacga ttcctttgat tgcgagttcg gtgatgagca
     3781 agaaaattgc ggcaggggcg gatgctattt tgctagacgt gactgtcggt gagggtgcct
     3841 tcatgaagac ggttgatgag gcgcgtgagt tggctcaaac tatggtagat cttggtaagg
     3901 ttgttggacg gaagacagta gcagtgatta cggatatgag ccagcctttg ggacgagcga
     3961 ttggaaatcg tctggaaatc cttgaagcat tggagatttt gcaaggtcaa ggacgtcagg
     4021 atattaccca ctttatttgt gaattggctc aaattatgct tggtttggca aatgttaaca
     4081 aaactgttga agaagttcgc caacatcttg aaaatggtca agcactggct aagtttgagg
     4141 aaatggtgca agcccaaggt ggagacttgg aagacctcta tcgtcctgta aatgtagctc
     4201 atgtggtgga aatccctgct caggaaacgg gtgtcatttc agctcttcca gctatggact
     4261 ttggtcttta tgccatgagg ctgggtgctg gtcgtgcagt taagtctgat gccttggact
     4321 atgaaacagg aattgttttt gaaaagaaga ttggagactc cgttcaaaag ggtgaaattg
     4381 ttgcaaaagt atacacaaat gaaaaaattc cacctcaact agttacagat ttccaaaaat
     4441 gtgttaaaat aagtgatgaa gtgaaaaaaa tccgtgaaat tgtagaaatc atctcctaaa
     4501 tggagggaaa accgaaatga aattaaataa atatatagat catacgcttt taaaacaaga
     4561 tgcaaagaaa aaacaaattg atagtttgtt gtctgaggct agagagtatg actttgccag
     4621 tgtttgcgtt aatccgacct gggttgaaca tgctaaaaaa ggacttgaag gcacagatgt
     4681 taaggtttgc acagtagtag gtttcccttt gggagcaaca acttcagccg tgaaagcatt
     4741 tgagacaaaa gaagctatcc aaaatggtgc agatgagatt gatatggtga tcaatgttgg
     4801 agatctcaaa tcaggtaatt tagccttggt tgagtcagat attcgcgcag tagtggaagc
     4861 aagtggtgat aagttagtga aagtcattat tgaagcttgc cttctgacag accaagaaaa
     4921 aattgttgtt tgccaattgg cccaaaaagc tggggctgac tttgtcaaaa catctactgg
     4981 cttttcaact ggtggtgcta cgatagcaga tgttacatta atgcgtgaaa cagttggatc
     5041 tgatatggga gtcaaggctg ctggtggagc tcgttcttat gcagatgctc ttgcctttgt
     5101 cgaagcaggt gcgacccgta tcggaacgtc agctggggta gctattttaa aaggagaatt
     5161 ggcagatggc gactactgag ttgattgaac tggcaattga aaccagcaaa catgcctatg
     5221 tcccctattc tcactttcct atcggagcag ttttagtagc caaagacgga aatgtttaca
     5281 cgggagtgaa tatcgaaaat gttagctatc ctttgaccaa ttgcggtgag agaacagcta
     5341 tttttaaggc tatatctgaa ggccaaagag aattttcaga attgattgtc tatggtcaaa
     5401 ctgaaaaacc gatttcacca tgtggtgctt gtcgccaagt aatggtcgaa ttttttgaac
     5461 aagatttaaa agtgacctta gtcgcaaaag ataaatcgac ggtcgagatg acggtcgggg
     5521 agttacttcc atattctttt acagacttaa attagtctga gtcgctcttt gagtggcaag
     5581 ggtctttgtg accaatcaat ccatacttgc aacatcgttg cacatcttat ttaggaggtt
     5641 cagtaatgaa caagaaacaa tggctaggtc ttggcctagt tgcagtggca gcagttggac
     5701 ttgctgcatg tggtaaccgc tcttctcgta atgcagcttc atcttctgat gtgaagacaa
     5761 aagcagcaat cgtcactgat actggtggtg ttgatgacaa atcattcaac caatcagctt
     5821 gggaaggttt gcaggcttgg ggtaaagaac acaatctttc aaaagataac ggtttcactt
     5881 acttccaatc aacaagtgaa gctgactacg ctaacaactt gcaacaagcg gctggaagtt
     5941 acaacctaat ctttggtgtt ggttttgccc ttcataatgc agttgaagaa gcagctaaag
     6001 aacacactga cttgaactat gtcttgattg atgatgtgat taaagaccaa aagaatgttg
     6061 cgagcgtaac tttcgctgat aatgagtcag gttaccttgc aggtgtggct gcagcaaaaa
     6121 caactaagac aaaacaagtt ggttttgtag gtggtatcga atctgaagtt atctctcgtt
     6181 ttgaagcagg attcaaggct ggtgttgcgt cagtagaccc atctatcaaa gtccaagttg
     6241 actacgctgg ttcatttggt gatgcagcta aaggtaaaac aattgcagcc gcacaatacg
     6301 cagccggtgc agatattgtt taccaagtag ctggtggtac aggtgcaggt gtctttgcag
     6361 aggcaaaatc tctcaacgaa agccgtcctg aaaatgaaaa agtttgggtt atcggtgttg
     6421 accgtgacca agaagcagaa ggtaaataca cttctaaaga tggcaaagaa tcaaactttg
     6481 ttcttgtatc tactttgaaa caagttggta caactgtaaa agatattgct aacaaggcag
     6541 aaaaaggaga attccctggc ggtcaagtaa tcgtttactc attgaaggat aaaggggttg
     6601 acttggcagt aacaaacctt tcagaagaag gtaaaaaagc tgtcgaagat gcaaaagcta
     6661 aaatccttga tggaagcgta aaagttcctg aaaaataatg gatggaagtc atttcttagg
     6721 aagcggccaa gggccgcttt tttaagagtt gagacaaagt cattttgtct cagtaaagct
     6781 tgctactaga caaactagca agttttattg aaataaaata agactctgaa aggaagagca
     6841 catggcacac gaaaatgtca ttgagatgcg tgatattacc aaggtgtttg gtggatttgt
     6901 tgccaacgac aaaatcaact tgcacctacg aaaaggtgaa attcatgcac ttttaggaga
     6961 aaatggggct ggtaagtcca cgctaatgaa catgttagca ggccttcttg aaccaactag
     7021 tggtgaaatc gcggtcaacg gtcaagttgt caatctcgac tccccatcta aagcagctag
     7081 cttgggaatc gggatggttc accagcactt tatgttggtt gaagccttca cagtggctga
     7141 aaacatcatt ttaggtagtg aattgactaa aaatggtgtg ctagatatcg ctggagctag
     7201 caaagaaatc aaggctcttt ctgaacgtta tggcttagct gttgaccctt ctgccaaggt
     7261 agcagatatc tcagttggag cccaacaacg tgtagaaatt ttaaaaacac tttatcgggg
     7321 ggctgatatc cttatctttg acgaaccaac ggctgttttg actccatcag aaattgatga
     7381 gttgatggct attatgaaaa atcttgtcaa agaaggaaaa tcaattatct tgattaccca
     7441 caaattggat gaaattcgcg cagtttctga ccgtgttaca gttatccgtc gtgggaaatc
     7501 aattgaaacc gttgaaattg caggggctac caatgctgat ttggcggaaa tgatggtagg
     7561 acgttctgtt tcctttaaaa cagagaagca agcctctaaa ccaaaagaag tggttttgtc
     7621 tatcaaagat ttggtggtca atgaaaaccg tggtgttcca gctgttaaaa atctgtcctt
     7681 ggatgttcgt gctggagaga ttgttggtat tgcggggatt gatggaaatg gtcagtctga
     7741 actgattcaa gccattacag gtcttcgtaa ggttgaatct ggtagcattg agctaaaagg
     7801 agattcaatt gtaggcttgc acccacgtca gattacagaa ctaagtgttg ggcacgttcc
     7861 agaagaccgt caccgtgatg gcttgatttt ggaaatgatg atatctgaaa atattgccct
     7921 tcaaacctac tataaagaac cacatagtaa aaatggaatt ttgaattatt caaatattac
     7981 ttcttatgct aaaaagctga tggaagagtt tgatgttcgc gctgccagtg aattagttcc
     8041 tgcagctgca ctctcaggag gaaatcaaca aaaagcaatt attgctcgtg aaattgatcg
     8101 agatcctgat ctccttatcg ttagccagcc aactcgtggg ttggatgtcg gtgccattga
     8161 gtatatccac aaacgcttga ttgaagagcg tgataatggc aaggctgtcc ttgttgtcag
     8221 ctttgaattg gatgagattt taaacgtctc agaccgtatt gccgttatcc acgatggtaa
     8281 gattcaaggt attgtatcac cagaaacaac caataaacaa gaacttggtg tcttgatggc
     8341 tggtggaaac ttgggaaagg agaagagtga tgtctaaaaa attacaacaa atttcggttc
     8401 ccttgatttc tgtatttcta ggaattttac tcggagccat tgtcatgtgg atcttcggtt
     8461 atgatgctat ttggggctac gaagaattgt tctatacagc ctttggcagt ctgcgtggga
     8521 ttggagaaat cttccgtgct atgggtcctc tggtcttgat tggtcttggt tttgcggttg
     8581 ccagtcgagc tggtttcttt aacgtcggac ttcctggtca ggctttggca ggttggattc
     8641 tcagtggttg gtttgcgctg tcgcatccag atatgccccg tcccttgatg attctagtaa
     8701 ccatcgtgat tgccttgatt gctggtggga ttgtcggagc gattccaggt attcttaggg
     8761 cctatctagg gacgtcagag gttattgtaa ccatcatgat gaactacatt gtcttgtatg
     8821 tagggaatgc ctttatccat gctttcccta aagacttcat gcaaagtaca gattcgacca
     8881 ttcgtgttgg ggctaatgca acctatcaga caccttggtt ggctgagttg actggtaact
     8941 cacggatgaa tattggtatt ttctttgcca tcattgccgt tgcagttatt tggttcatgc
     9001 tcaagaaaac aactcttggt tttgaaatcc gtgcagttgg tcttaatcca catgcttcag
     9061 aatatgctgg tatttctgcc aagcggacta ttatcctatc tatgattatt tcaggtgcct
     9121 tggcaggtct tggtggagct gttgaaggtt tgggaacctt ccagaacgtc tatgttcaag
     9181 gttcgtcatt agctatcgga tttaacggaa tggcggttag tttgcttgcg gccaactcac
     9241 caattggtat actctttgca gccttcctat ttggcgttct ccaagttggg gctcctggta
     9301 tgaatgcggc gcaggtacca tctgagcttg tcagcattgt aacagcgtct attatcttct
     9361 ttgtcagtgt tcattacctt atcgaacgct ttgtcaaacc gaaaaaacaa gttaaaggag
     9421 gtaagtaaag atgtctatta taaccttgct ccccctcttg gtgtcttcta tgctgattta
     9481 ctcagcacct ctcatcttta caagtatcgg tggtgttttc tctgaacgtg gtggtgtggt
     9541 aaacgttggc cttgaaggaa ttatggttat gggtgccttt tctggagttg tctttaacct
     9601 tgaatttgca gaacaatttg gagcagcaac tccatggcta tccttgcttg tagcaggatt
     9661 ggttggtagt gttttctcta tcatccacgc agcagcgacg gttcatttcc gtgcagacca
     9721 tgttgtcagc ggtacggtat tgaacttgat ggcgcctgcc ttggctgttt tcttagttaa
     9781 agttctttat aacaaaggac aaaccgacaa cctaagtcaa acttttggac gctttgattt
     9841 cccagtcttg gcaaatatcc cagtgattgg tgatatcttc ttcaagtcaa ctagtctact
     9901 tggttatctg gcgattgcct tctcattcct tgcttggttt attctcttta agactcaatt
     9961 tggtcttcgt ctccgctctg tcggtgaaca tcctcaagca gcggatacct tgggaatcaa
    10021 cgtctacaag atgagatatt tagggattat tatttcaggt tttctaggtg gaattggcgg
    10081 agcgatttat gcgcaatcaa tctcagttaa cttctcagtg acaactattg ttggacctgg
    10141 atttatcgcc cttgctgcga tgatctttgg gaaatggaat ccaatcggtg ctatgctttc
    10201 tagtctcttc tttggacttt cacaaagttt ggctgttatc ggttctcaat tgccgttcct
    10261 acaaggagtg ccagcggttt atcttcaaat tgcaccttat gttttgacaa ttcttgtctt
    10321 agcagccttc tttggaaaag cagtcgcacc aaaagcagat ggtatcaact acatcaaatc
    10381 aaaataagca tacaaaaaaa cgtcagtcca atgactgaac tgccccccaa aagttagaca
    10441 gaaaaaatct aacttttggg gtg
//
LOCUS       DAVBGY010000062        10317 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVBGY010000062 DAVBGY010000000
VERSION     DAVBGY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10317
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.062"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            848..1246
                     /locus_tag="VQ620_001734"
     CDS             848..1246
                     /locus_tag="VQ620_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET2764321.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEVGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKKQTVFLILQQ"
     gene            1284..1973
                     /locus_tag="VQ620_001735"
     CDS             1284..1973
                     /locus_tag="VQ620_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2764322.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNFV
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            2015..2263
                     /locus_tag="VQ620_001736"
     CDS             2015..2263
                     /locus_tag="VQ620_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764323.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLKWFVLERLELKDDA"
     gene            2293..2904
                     /locus_tag="VQ620_001737"
     CDS             2293..2904
                     /locus_tag="VQ620_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2764324.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            3065..3859
                     /locus_tag="VQ620_001738"
     CDS             3065..3859
                     /locus_tag="VQ620_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET2764325.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3856..4491
                     /gene="trmB"
                     /locus_tag="VQ620_001739"
     CDS             3856..4491
                     /gene="trmB"
                     /locus_tag="VQ620_001739"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET2764326.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            4617..5096
                     /gene="rimP"
                     /locus_tag="VQ620_001740"
     CDS             4617..5096
                     /gene="rimP"
                     /locus_tag="VQ620_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET2764327.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            5140..6276
                     /gene="nusA"
                     /locus_tag="VQ620_001741"
     CDS             5140..6276
                     /gene="nusA"
                     /locus_tag="VQ620_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HET2764328.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            6298..6591
                     /locus_tag="VQ620_001742"
     CDS             6298..6591
                     /locus_tag="VQ620_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HET2764329.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            6584..6883
                     /locus_tag="VQ620_001743"
     CDS             6584..6883
                     /locus_tag="VQ620_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HET2764330.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6900..9692
                     /gene="infB"
                     /locus_tag="VQ620_001744"
     CDS             6900..9692
                     /gene="infB"
                     /locus_tag="VQ620_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET2764331.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDSRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            9943..10293
                     /gene="rbfA"
                     /locus_tag="VQ620_001745"
     CDS             9943..10293
                     /gene="rbfA"
                     /locus_tag="VQ620_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET2764332.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3153 a         1876 c         2328 g         2960 t
ORIGIN      
        1 ataatttaat tagaaaaata ttttcaaaat ttcccaaaaa ggatacagat agtaccccct
       61 aaaaaataaa tttctcccct gtatttgcta gcttaattta caggggagtt tctttaatat
      121 tgtttcgcct gtcaaaatga tatgtgatag caggatgaaa agtctacaaa caagttggta
      181 tcaatacaat ataaagagga aagtatgttt ttaatgctta ctttttaaat tttttaacaa
      241 ttcaagatgt ttcgatgaca attcaagatt tggatgaaaa attttaaaaa acaatgatat
      301 actaaacttg tcaaagttgc aacgggtgct gctacattat gttaaaaatc gctcttgatt
      361 gataagctgg atttgttagg aaaggagtaa ataattatgg ataagaaaaa gatagtttcg
      421 actataatat gtatagtatt tcttgtagtg tcagtagata atttttttag agacttaacc
      481 ccgttactgt tcattttaaa tattattggc ctgtcttgct tttcagtgct aacatacatt
      541 aacataaaag agatactctt aaatataagt aaatgatgat ttggttagtc ctagaaccaa
      601 tcaacttatg tttatcggct tggcagattt tatgtttgtc atttgtttgt atagaggtat
      661 ttcagaaaca gaattctatc aacaactaat cgcttatata ggtgtattct ctgcttgttt
      721 atcacgcttc tgctcatgtg gggcttgaag tattataaaa agtagctttc tatccttttc
      781 tgacaggata tttgagtaga aggctctatt aaattgttag tgaaataaat gaatggaggt
      841 atttaatatg aaatacagat tattttttgt tattttcttg agtagtgtgt tggatattct
      901 tttagggaca tttttacaaa tctctatcgt atctattggg tggcttgttc tttacagtgg
      961 tttgtttgaa gtaggagttt tccttcttgc taataaaggg gtggcggtaa aaatcaagga
     1021 agtagatatt cgaaatcgct ttaaatttat ttttggaaaa accttatggt ttcaaattct
     1081 tttgctcatc tttttgataa tcaaacttta tcttggtttg gatgcgaggt tgattttatt
     1141 ctatggacat attttcattg tctttaatgc cttaatgtat ctattatcta gtagtcaggt
     1201 tagccttaaa aaaaaacaaa ctgtcttctt aatcttacag cagtagagtc tgacaaggtg
     1261 aggtcatagt agggaggatt tctatgaaaa agtatcaact tctattcaaa ataagtgcag
     1321 tcttctctta cttatttttc gtatttggtc tttctcagct gacgcttatc gtccaaaatt
     1381 attggcaatt ttcttcccag attggtaatt tcgtctggat tcaaaatatc ttgagtttac
     1441 tttttattgg agtcatgatt gtggttcttg ttaagacagg ccatggttat ctcttccgca
     1501 ttccaagaaa aaaatggctt tggtattcga ttttgacagt attagtggta gtgttccaga
     1561 tctcttttaa cgttcagaca gctaaacatg ttcagtcaac tgcggaaggt tgggctgtat
     1621 tgattggtta tagtgggact aactttgcag agctaggtat ttatatagcc ctgttctttc
     1681 tggttccact gatggaagaa ttgatttata gaggattact gcaacatgct ttctttaagc
     1741 attcgcgatt tggtcttgat ttgcttcttc cttctatttt atttgctctc cctcattttt
     1801 caagcctgcc tagtctgtta gatatcttcg tctttgcaac agttggaatc atctttgctg
     1861 gtttgacccg ctataccaag agcatttatc catcctatgc ggtgcatgtg atcaataata
     1921 ttgtagcgac cttcccgttt ttgctcactt ttctacatag ggtcttgggg taaaaaaaca
     1981 aaaggcttgc ttttcagcca tagaggaggt catcatgtat aaacacttat ttttcctaga
     2041 ttccaaaact ttagatcggt tgacacccta tattctagtc ttggcttctg acaccattgc
     2101 ttttaatgtt tttgtgctaa cctttgtatc tgcggtggtt tttaatttcc taaattccat
     2161 gctagcttta atggctatat tcataggggc tggctatgtg gtcggatttt ggttgctaaa
     2221 atggtttgtt ttggaaaggt tagagctaaa ggatgacgcg taggaagtct gtttggtgag
     2281 gaggatagtt ttatggagtt ttttgataaa tttcatgcct tgtgttttgg atttttagta
     2341 ctaataattg tcattacagt tccttatacg attaaccatg ggggtttttt tcaaaatgaa
     2401 tctgcattga ttcttgtaag tcttcttgta acctcgctga gtgttgctta tgctagaaag
     2461 tttgaaatga tttcttttgg gatgttaagc aagaaacaac ttttgctttt cattgcaatc
     2521 tttcttctaa gtgtacttga gacgctggtt tatattcatt tcttcgctgt ttcttctggc
     2581 tcaggggtcc aacacttggc ggaagtcagc agaggaattt ccctgtcttt gattttgact
     2641 acctcagttt ttggccccat ccaggaggaa ctcattttca gaggacttct tcaaggtgcg
     2701 gtttttgaca attcttggtt agggcttgtg ctaacttcct ctctcttttc tttcatgcat
     2761 ggaccttcta atgtcccttc gtttattttt tatctacttg ggggtttgtt gctgggcttt
     2821 gcttataaaa agagtcaaaa cctatgggtt tctactctag tacacatgtt ttacaacagt
     2881 tggccactcc tatattattt ataaaaatta tgaaaaggta agcagaagac ggtgcttacc
     2941 tttttcaagc tataatgata cccaagccaa tccagaggct tttctttgag aatcgggtta
     3001 gagtggttga cgaataggcc aaaaactagt agaatagtaa ggaaacttta tacggaggaa
     3061 agaaatggat ttgggtgata atgagctaac actgactccc atacctggga aaagtggcaa
     3121 ggcttatatg ggtagctatc ctgacgggaa gcgcatcttt gtaaaaatga acacctctcc
     3181 aatcctacct ggtctagcta gagaacaaat tgctccacaa ttattatgga gtcgtcgttt
     3241 ggcagatggg cgtgatatgt gtgctcaaga atggttgaca ggcaagatat tgacccccta
     3301 tgatatgaat cgtaagcaaa tcgtcaatat tttaacccgt cttcatcgct cacgtccgtt
     3361 gatgacacaa ttgagtcgtt tgggctatgc catggaaaca cctgtagatt tactacagtc
     3421 ttggcaggaa acggctccag atgctttgcg taaaaatcat tttatcagtg aagtgatggc
     3481 tgatttacgt cagactattc caggatttag agaggaccat gcgaccattg tccatggaga
     3541 tgtacgacat agtaattgga ttgagacaga tagtggcttg atttatttag tagattggga
     3601 ttcggttcgc ttgaccgatc gcatgtttga tgtggcccat atgctctgcc attatatttc
     3661 agaacatcag tggaaggaat ggttgaccta ctacggttac aagtacaatc aaacggtatt
     3721 aagtaaattg tattggtatg gtcaattgtc ttatttgagt cagatttcca agtattatat
     3781 gaaccaagat ttagaaaatg tcaatcggga gattcatggt ttgcgtcatt tccgagacaa
     3841 gtatggaaag agaagatgag agttagaaat cgtaaagggg caacagaatt actagaggca
     3901 aatccccagt atgtggtcct caatcccttg gaagccaagg caaaatggcg ggacttgttt
     3961 ggcaatgata atcccattca tgtggaagtt ggaagtggaa agggtgcctt tgtttcaggt
     4021 atggccaagc aaaaccctga catcaactat atcgggattg atattcaaaa gtctgttttg
     4081 agctacgctt tggacaaggt gcttgaagtt ggagtgccta acatcaagct cttgtgggta
     4141 gatggttctg acttaactga ctattttgaa gacggtgaga ttgatcgctt gtatctgaac
     4201 ttttcagatc catggccgaa aaaacgccat gaaaagcgtc gtttgaccta caagaccttc
     4261 ttggatacct tcaaacgtat cttgcctgaa aatggagaaa ttcatttcaa gacggataac
     4321 cgtggcttgt ttgagtacag tttagtgagc ttttctcaat atggcatgaa actcaatggt
     4381 gtctggttag atttgcatgc cagtgatttt gaaggcaatg tcatgacaga atacgagcaa
     4441 aaattctcaa acaaggggca agttatctac cgagttgagg cagaatttta agagatagcc
     4501 taaaattagg ctgtacaagt gcttttgctt tacataagtt ggcaaacgtg ctatactgat
     4561 agtaagaata tgaaaagtga ggcggggaaa tatcttcgcc tcttgcttat gaggaggtgg
     4621 acgcaatcgc aacaatcgta gaattagtca gagaagttgt agaacctgtc atagaagctc
     4681 cttttgaact cgtggatatc gagtatggaa agattggcag tgacatgatt ctcagtattt
     4741 ttgtagataa acccgaagga attaccttga acgacacggc agacttgaca gaaattatca
     4801 gtcctgtcct agacaccatc aagccagatc ccttcccaga acaatatttc ctagaaatta
     4861 ccagtccagg tttggaacgc cctttgaaaa ccaaggatgc cgtcgctgga gcggttggaa
     4921 aatacatcca tgtcgggctc taccaagcca tcgataagca aaaggtcttt gaaggaacct
     4981 tgttggcctt cgaagaggac gagttgacta tggaatatat ggacaagacg cgtaagaaaa
     5041 ccgtccaaat tccatacagt ttagtatcaa aagcacgttt agcagttaaa ttatagaaaa
     5101 agaaaggata gcttttgagg attcaaaagt gaagaaaaca tgagtaaaga aatgctagag
     5161 gccttccgca ttttggaaga agacaaggga atcaaaaaag aagatatcat cgacgcagta
     5221 gtagagtcgc ttcgttccgc ttatcgcaga cgctatggtc agtcagacag cgtagctatt
     5281 gacttcaacg aaaaaacagg tgactttaca gtttatactg tccgtgaagt tgttgatgaa
     5341 gtatttgata gccgtttgga aatcagcttg aaagatgctc ttgccattaa ttcagcttat
     5401 gaacttggag acaaaatcaa gtttgaagaa gcaccagctg agtttggtcg tgtagcagcc
     5461 cagtctgcca aacaaaccat catggaaaaa atgcgcaagc aaacacgtgc catcacttac
     5521 aatacttaca aagaacatga gcaagaaatc atgtctggta cagtagaacg ctttgacaac
     5581 cgctttatct atgtcaacct tggtagcatc gaagcccaat tgtcaaaaca agaccaaatt
     5641 cctggagaag tttttgcttc tcatgatcgt atcgaagttt atgtttacaa ggttgaagac
     5701 aaccctcgtg gtgtgaacgt ctttgttagc cgtagtcatc cagaaatgat caaacgttta
     5761 atggagcaag aaattccaga agtttatgat ggaactgttg aaatcatgag cgtggctcgt
     5821 gaagcaggtg accgtacgaa ggttgctgtt cgtagccaca atccaaacgt ggatgctatc
     5881 ggtacaatcg ttggacgtgg tggtgctaat atcaagaaga ttactagcaa attccaccca
     5941 gctcgttacg atgctaaaaa tgaccgcatg gtaccaatcg aagaaaatat cgatgttatc
     6001 gagtgggtag cagatccagc tgaatttatc tacaatgcca tcgctcctgc tgaggttgac
     6061 caagttatct ttgatgaaaa cgacagcaaa cgtgccttgg tggttgttcc agataacaag
     6121 ctttctcttg ccattggtcg tcgtggacaa aacgtgcgct tggcggctca cttgactggt
     6181 taccgtatcg atatcaagtc tgctagcgaa tttgaagcca tggaagacgc tgcttcagta
     6241 gagttggaag tagaaaacga tactgtagaa gaataaaagc tgctagagga gggaaagatg
     6301 aaaacaagaa aaatcccttt gcgcaagtct gttgtgtcta acgaagtcat tgataagcgt
     6361 gatttgctcc gcattgtcaa gaacaaggaa ggacaagtct ttattgatcc tacgggcaag
     6421 gccaatggcc gcggcgctta tatcaaacta gacaatgcag aagccctaga ggcgaaaaag
     6481 aagaaggtct ttaaccgcag ctttagcatg gaagtggaag aaagctttta tgacgagttg
     6541 atcgcttatg tggatcacaa agtgaaaaga agagagttgg gacttgaata agcaaaagat
     6601 aagtaatctc ttggggcttg ctcagcgagc agggcgcatc atatcgggtg aagaattggt
     6661 ggtcaaggcc attcaagacg gcaaggccaa gttggtcttt ctagctcatg atgctggacc
     6721 caatctgacc aagaagattc aagataaaag tcattattat caagtagaaa ttgtaaccgt
     6781 gttttcaaca ctggaattaa gcatagcagt cgggaaatcg agaaaggttt tggctgtaac
     6841 agatgctgga tttacaaaga aaatgaggtc tcttatggaa tagaagagga ggacatgatt
     6901 tgtctaagaa aagattgtac gaaatcgcaa aagaacttgg aaaagaaagt aaagaagttg
     6961 tagcgcgtgc aaaagagttg ggcttggatg tgaaaagcca ctcatcaagt gtggaagaag
     7021 ctgtcgctgc aaaaattgct gccagcttta agcctgcagc tgctccgaaa gtagaagcaa
     7081 aacctgcagc accaaaagta agtgcagaaa agaaagccga aaaatctgag ccagctaaac
     7141 cagctgtagc taaggaagag gcaaaaccgg ctgagccagt tgctccgaaa acagaaaaag
     7201 tagcagtgaa accgcaaagt cgtaatttca aggctgagcg tgaagcccgt gctaaagagc
     7261 aggcagaacg acgcaagcaa aataagggca ataaccgtga ccaacaacaa aacggaaacc
     7321 gtcagaaaaa cgacagccgt aatggtggaa aacaaggtca aagcaaccgc gacaatcgtc
     7381 gctttaatga ccaagctaag aagcagcaag gtcagcaaaa acgtagaaat gagcgccgtc
     7441 agcaagagga taaacgttca aatcaagcgg ctccacgtat tgactttaaa gcccgtgcag
     7501 cagccctaaa agcagagcaa aatgcagagt acgctcgttc aagtgaggaa cgcttcaagc
     7561 agtatcaggc tgctaaagaa gccttggctc aagctaacaa acgcaaggaa ccagaggaaa
     7621 tctttgaaga agcggctaag ttagctgaac aagcacagca agttcaagca gtggttgaag
     7681 tcgtccctga gaaaaaagaa cctgcagtgg atacacgtcg taaaaaacaa gctcgaccag
     7741 acaaaaatcg tgacgattat gatcatgaag aagatggtcc tagaaaacaa caaaagaatc
     7801 gaagtagtca aaatcaagtg agaaatcaaa agaatagtaa ctggaataac aacaaaaaga
     7861 acaaaaaagg taataacaag aacaaccgta atcagactcc aaaacctgtt acggagcgta
     7921 aattccatga attgccaaca gaatttgaat atacagatgg tatgaccgtt gcggaaatcg
     7981 caaaacgtat caaacgtgaa ccagctgaaa tcgttaagaa acttttcatg atgggtgtca
     8041 tggccacaca aaaccaatcc ttggatgggg aaacaattga actcctcatg gtggattacg
     8101 gtatcgaagc caaacaaaag gttgaagtgg ataatgctga catcgaacgt ttctttgtcg
     8161 aagatggtta tctcaatgaa gatgaattgg ttgagcgtcc accagttgtt actatcatgg
     8221 gacacgttga ccacggtaaa acaacccttt tggatactct tcgtaactca cgtgttgcga
     8281 caggtgaagc aggtggtatt actcagcata tcggtgccta ccaaatcgtg gaaaatggta
     8341 agaagattac cttccttgat acaccaggac acgcggcctt tacatcaatg cgtgcgcgtg
     8401 gtgcttctgt taccgatatt acgatcttgg tcgtagcggc agatgacggg gttatgcctc
     8461 agactattga agccatcaac cactcaaaag cagctaacgt tccaatcatc gtagccatta
     8521 acaagattga taaaccaggt gctaacccag aacgcgttat cggtgaattg gcagagcatg
     8581 gtgtgatgtc aaccgcttgg ggtggagatt ctgaatttgt tgaaatctca gctaaattca
     8641 accaaaatat cgaagaattg ttggaaacag tccttcttgt agctgaaatc caagaactca
     8701 aggcagaccc aacagtgcgt gcaatcggta cggttatcga agcgcgcttg gataaaggaa
     8761 aaggtgcggt cgcaaccctt cttgtacaac aaggtacctt gaatgttcaa gacccaatcg
     8821 ttgtcggaaa taccttcggt cgtgtccgtg ctatgaccaa cgaccttggt cgtcgtgtta
     8881 aagttgctgg accatcaaca ccagtctcta tcacaggttt gaacgaagca ccgatggcgg
     8941 gtgaccactt tgccgtttac gaggatgaaa aatctgcgcg tgcagcaggt gaagagcgtg
     9001 ccaaacgtgc cctcatgaaa caacgtcaag ctacccaacg tgttagcctt gaaaacctct
     9061 ttgataccct taaagctggg gaactcaaat ctgttaatgt tatcatcaag gctgatgtac
     9121 aaggttctgt tgaagccctt tctgcctcac ttcaaaagat tgacgtggaa ggtgtcaaag
     9181 tgactatcgt ccactcagcg gtcggtgcta tcaacgaatc agacgtgacc cttgccgaag
     9241 cttcaaatgc ctttatcgtt ggtttcaacg tacgccctac accacaagct cgtcaacaag
     9301 cagaagctga cgatgtggaa atccgtcttc acagcattat ctacaaggtt atcgaagaga
     9361 tggaagaagc tatgaaaggg atgcttgatc cagaatttga agaaaaagtt attggtgaag
     9421 cggttatccg tgaaaccttc aaggtgtcta aagtgggaac tatcggtgga tttatggtta
     9481 tcaacggtaa ggttgcccgt gactctaaag tccgtgttat ccgtgatggt gtcgttatct
     9541 atgatggtga actcgcaagc ttgaaacact ataaagacga cgttaaggaa gttacaaacg
     9601 gccgtgaagg tggattgatg atcgacggct acaatgatat taagatggat gatgtgattg
     9661 aggcgtatgt catggaagaa atcaagagat aagatttttt gctcctttct taggtggtga
     9721 gggacgcaag caaaccgatg gtttcattgc ttatttttga gcctagggtc tcaaaaatcc
     9781 cctgtgatgg gactgataaa tcagttccat cactttcacc acggcgaaag aagcagatga
     9841 cttcaaattg aacttcgttt caatttaaac tgaaaatcaa gaagtttaaa atagctaggt
     9901 ctgctggcct agcttttggt tcaaagtaga gaaaggaata tcatggcaaa tcatttccgt
     9961 acagatcgtg tgggcatgga aatcaagcgt gaagtcaatg agattttgca aaagaaagtc
    10021 cgtgatccac gtgtccaagg tgtgaccatc acagatgttc agatgctggg tgacttgtct
    10081 gttgccaagg tttattacac cattttgagt aaccttgctt cggataacca aaaagcccaa
    10141 atcgggcttg aaaaagcaac tggtaccatc aaacgtgaac ttggtcgcaa tttgaaattg
    10201 tacaaaatcc cagatttgac cttcgtcaaa gacgagtcca tcgagtatgg aaacaagatt
    10261 gacgagatgc tacgcaatct ggataagaac taaagaagag gggttgcccc tcttttt
//
LOCUS       DAVBGY010000063        10100 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVBGY010000063 DAVBGY010000000
VERSION     DAVBGY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10100
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.063"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..70
                     /locus_tag="VQ620_001746"
     CDS             <1..70
                     /locus_tag="VQ620_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2764333.1"
                     /translation="NKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(198..1220)
                     /gene="cbpF"
                     /locus_tag="VQ620_001747"
     CDS             complement(198..1220)
                     /gene="cbpF"
                     /locus_tag="VQ620_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
                     /protein_id="HET2764334.1"
                     /translation="MKLLKKMMQIALATFFFGLLATNTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDPLSGEMVVGWQYIPAPHKGVTIGPSPRIEIALRPDWF
                     YFGQDGVLQEFVGKQVLEAKTATNTNKHHGEEYDSQAEKRVYYFEDQRSYHTLKTGWI
                     YEEGHWYYLQKDGGFDSRINRLTVGELARGWVKDYPLTYDEEKLKAAPWYYLDPATGI
                     MQTGWQYLGNKWYYLHSSGAMATGWYKEGSTWYYLDAENGDMRTGWQNLGNKWYYLRS
                     SGAMATGWYQEGSTWYYLNASNGDMKTGWFQVNGNWYYAYDSGALAVNTTVGGYYLNY
                     NGEWVK"
     gene            complement(1239..1749)
                     /locus_tag="VQ620_001748"
                     /pseudo
     CDS             complement(1239..1749)
                     /locus_tag="VQ620_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
     gene            complement(1797..2147)
                     /locus_tag="VQ620_001749"
     CDS             complement(1797..2147)
                     /locus_tag="VQ620_001749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764335.1"
                     /translation="MIKIDNTLQYPYSTSAMVLSKYYGVADGMNVEGRGSANFIKDNV
                     LITAAHNYYRHDYGKEADDIYVLPAVSPSQEPFGKIKVKEVRYLKEFRNLNSKDAREY
                     DLALLILEKPIVQN"
     gene            complement(2144..2383)
                     /locus_tag="VQ620_001750"
     CDS             complement(2144..2383)
                     /locus_tag="VQ620_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764336.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(2533..2799)
                     /locus_tag="VQ620_001751"
                     /pseudo
     CDS             complement(2533..2799)
                     /locus_tag="VQ620_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(2902..3126)
                     /locus_tag="VQ620_001752"
                     /pseudo
     CDS             complement(2902..3126)
                     /locus_tag="VQ620_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002877158.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3051..3294)
                     /locus_tag="VQ620_001753"
                     /pseudo
     CDS             complement(3051..3294)
                     /locus_tag="VQ620_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3295..3927)
                     /locus_tag="VQ620_001754"
     CDS             complement(3295..3927)
                     /locus_tag="VQ620_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2764337.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYMI
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(3941..4936)
                     /locus_tag="VQ620_001755"
     CDS             complement(3941..4936)
                     /locus_tag="VQ620_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2764338.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSMTCLLVLFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(4933..5631)
                     /locus_tag="VQ620_001756"
     CDS             complement(4933..5631)
                     /locus_tag="VQ620_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HET2764339.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(5709..6719)
                     /locus_tag="VQ620_001757"
     CDS             complement(5709..6719)
                     /locus_tag="VQ620_001757"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HET2764340.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEANDQMK
                     KA"
     gene            complement(6703..7710)
                     /locus_tag="VQ620_001758"
     CDS             complement(6703..7710)
                     /locus_tag="VQ620_001758"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HET2764341.1"
                     /translation="MIAVKTCGKLYLAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIVLMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLTSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(7697..8650)
                     /gene="mvaD"
                     /locus_tag="VQ620_001759"
     CDS             complement(7697..8650)
                     /gene="mvaD"
                     /locus_tag="VQ620_001759"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET2764342.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMAFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(8632..9510)
                     /gene="mvk"
                     /locus_tag="VQ620_001760"
     CDS             complement(8632..9510)
                     /gene="mvk"
                     /locus_tag="VQ620_001760"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HET2764343.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(9632..9961)
                     /locus_tag="VQ620_001761"
     CDS             complement(9632..9961)
                     /locus_tag="VQ620_001761"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2764344.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMIPHDFPLYLTVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
BASE COUNT         2943 a         2376 c         1772 g         3009 t
ORIGIN      
        1 caacaagaga attaaggtaa aactaaaagg acttagtcct gtgcagtaca gaactaaatc
       61 ctttggataa attatttgtc taacttttgg ggggcagtac agaaaaacct tgatactatg
      121 cgttttttga ttgtgaagac ttttatccac ctattataca aagttaagta tccatcacag
      181 tttacaatta gccttcatta cttaacccat tcaccattat agtttaagta gtaaccacct
      241 actgtggtat taacagctaa agcacctgaa tcataggcat agtaccagtt accattgact
      301 tggaaccagc ctgttttcat atctccatta cttgcattta gatagtacca agtcgaacct
      361 tcctgatacc aaccagttgc catagctcct gatgaacgga gatagtacca tttgttccca
      421 aggttttgcc agccagttct catatcacca ttttcagcat ctagatagta ccaagttgag
      481 ccttccttat accagccagt tgccatagct cctgacgaat ggaggtagta ccacttatta
      541 cctagatatt gccaacctgt ttgcataatg ccagttgctg gatctagata gtaccatgga
      601 gctgctttta gcttctcttc atcatacgta agagggtaat ccttaaccca accacgtgct
      661 agctctccaa ccgtcaatct gttgatgcgc gaatcaaagc caccatcctt ctgtaaataa
      721 taccaatgac cctcttcata aatccaacca gtttttaaag tatgataact acgctgatct
      781 tcaaaataat agactcgttt ctctgcttgg ctatcatatt cttccccatg atgtttgttg
      841 gtattcgtag cagtttttgc ttctaaaact tgcttgccaa caaattcttg taatacacca
      901 tcttgaccaa aataaaacca atctggtcta agagcaatct ctattcttgg agaaggacca
      961 atcgtaaccc ccttgtgtgg agcaggtata tattgccagc cgacaaccat ctctccggat
     1021 aaaggatcaa aataatagta cttcccatct atcactctcc agtaggtttc ttttagatcc
     1081 ccctttttgt agtaggttct accattttct tggacaaact gccatccttc agaatcatct
     1141 gcaaatactg tatttgtcgc taacaaaccg aagaaaaatg tggctagtgc gatttgcatc
     1201 atttttttca aaagcttcat tttatatatc ctccaacatt aaatccactc accagatgaa
     1261 gcgaagttat aaactttacc atcgatagtt tggctacctg taaccattgc tccatttgaa
     1321 ttgagataat accattttcc atttactttt tgccaatctg taagcatctt accggaactg
     1381 tctaaataat accaagtatc atttatctcc tgccaacccg tttgtttatc atgttgtcta
     1441 taatggtacc aactaccatt tattttcttc catccttcaa aagagtaacc tttaagaacc
     1501 gaataaataa ataaatggca aatttcgttc atttaattta actgcactgt taatttgatt
     1561 agatccatct cctaaagtat gcactcctac tacgcggtta ctagcatcat aaactgtaga
     1621 tccactagac ccctctaaag tatcaacttg gtagtccaag aacatgccat catcacttaa
     1681 aacttgtttt ttatctgtat acatttgatg aattttaaaa ttatatgatg gatagcctgt
     1741 gatagtcaca gttattcctg tcaaattttt ttgactagta ggaagaccca aagtccctaa
     1801 ttttgcacaa tgggcttttc tagaattaat aaagccaagt catattccct tgcatcctta
     1861 gaatttaaat ttctaaattc cttcaaataa cgaacttcct ttactttgat ctttccaaat
     1921 ggttcttgac ttggactaac agccggaaga acataaatat catccgcttc tttcccatag
     1981 tcatgtctgt agtagttgtg agccgctgta attaacacat tatctttaat aaaattcgca
     2041 cttcccctcc cttctacatt cattccatcg gctactccat aatacttaga taaaaccata
     2101 gctgaagtcg aatacggata ctgtaaagta ttatcaattt taatcaaatc atcattaccg
     2161 ataatacttc tgattgcttt tggtagtatg aaccatatgt tggtgaaatc tcagataatg
     2221 aagaatcatt agatcctgga cctttttcta gtgtctcact tacctcatat tcttcacccg
     2281 tactagaaat aacactcaaa gcagatactg tcgataactg gctagccaat aaagtactcg
     2341 caataattga aatacccaat tttttataaa cagttttctt cattattgta gcctcctaat
     2401 gtaattatag cgtactattc taaatttctt aatctactat agaatcaaga aatctaccac
     2461 cttctttaaa taccctccat tatcacataa acaggtaaac ttttcaatta atgactgcgc
     2521 ttttcaatca cgctagaggt acttacttgc ttctttgata ctaagttcag ccattctttc
     2581 cttgtttttc tcaataaagc atgttaccca agtgggattc gttttggagt agtttcgcag
     2641 aatccagcca atggctttat tgataaaaaa ttctgtttgg ttcaagttat gaagtagaat
     2701 cttttccatt aattgagtat tggtcttctc ttttcttaac aactggtggt caatagcgac
     2761 acgtctcagc cagatattat ctgataggct ccattttata ctcaatgaaa atcaaagagc
     2821 aaactaggaa gctagccgca ggctttactt gagtacggca aggtgaagct gacgtggttt
     2881 gaagagattt tcgaagaata ttaagattat ttcttctagt tcagggtgtt catacaccaa
     2941 actccctact actcgatcta ggatatctac cgtgtcccac aaggattttg tcacgactaa
     3001 ctgctctagc ttaggcaaat cggtttcctt tagataagac tgcattgctt tcaaatagtt
     3061 agcaaccaca tattggtatt ttctaggctc cttttcccag caagtgtctg caaaatccca
     3121 atcgataatc tttgtttttt tcgcttctgg aaaatatttt tatagagttt atttctttca
     3181 ggcaccgcaa tacctaaaaa aagaaaattg atggcgcata taggcttcca tggaccttgc
     3241 ttttttagag tcttttgctg cttctagctc ctcaagtaaa tctgctaaac tcatctaaaa
     3301 ctcctcttgc cccaccaaat ggtgctgaaa ggcatagaca gccgcctggg tacgatcgct
     3361 gacttcaagt ttggcaagaa tattggacac gtgggtcttg accgtcttga gagagataaa
     3421 gaggtcatct gcgatgcgct gattttcgta gcccttggcg atgagttgga gaacatctcg
     3481 ctcacgcgca gtcaattctt catgaagttc catatgattg cggtggtatt caaccttctt
     3541 gctaacctct tgctcaatgg ccagctcgcc agcagctacc ttactgacgg catgaagcaa
     3601 ttcatctgca ctagaagtct tgatcatata gcctttggca ccagcatcta agactggcat
     3661 gattttttca ttatccaaat aagaggtcac aatcaaaatc ttggcttcag gccattcttt
     3721 aaggattgct aaggtcgcgt caatcccatt catctcaggc atgacaatat ccatgacaat
     3781 gacatctgga cgcagttcca aggccaagtc aatcccttga gacccgttgg acgcctcacc
     3841 cacaacttct acatcgtctt ggaggtcaaa gtagcttttc aagcccaaac ggaccatttc
     3901 atggtcatct actagtaaaa ttttcatctt tactccttta tcattcctta tctaacaggg
     3961 gaatacggat atcaactgcc agcccttgct tgggagctgt taataactga accgtccctg
     4021 ccatatcttc aacccgctcc ttgatatttc gcagtccata actcaagtcg tctaagctcc
     4081 ctaactggaa accaatccca ttgtccacca ccttcagttg caattcaaca tctgtctgat
     4141 agaggtagac atctaggcaa gatgcctggg catggcggag cgtattgcta atcaactctt
     4201 gcaggatacg gaaaatatgc tcctcgattt tcttaggcaa tttcgtcata ttctgcttga
     4261 gactaaccct aagatcactc ttgtcctcaa gctcttttaa gagaatttga atcccttcta
     4321 tcaagctctt ctgctccagt tcaactggtc gcaaatgcaa gagcaaaacc cgcaaatcct
     4381 tctgggctgt ttctaaaata gctgtgacac tctgcaactg ggtctgcatc ttttctctat
     4441 ccaatttcaa agcctgctga ctgatacccg ataaaatcat gtgggccgca aacaactcct
     4501 gactgactgt atcgtgcaaa tcccgagcaa ttcgcttccg ttccttctcg atgatttcct
     4561 cttcctgagc aaggctatga ttttcagctt tttgaagagc ctctgtcaaa aggttaagtt
     4621 tacctgataa ggacttgaaa ctggcatcca aatctggatc tacaacctga actacttctt
     4681 gccctgccaa taaacgcttg agattagcct gcatttttct tagagaaagc tcttcgatcc
     4741 ctcgccaaaa caggactaag agacaggtca tggacatgct gaaaaccaac aataaaaaga
     4801 caaatttttc tgttttttcg acatcgtgca aaaagataga ccagtcaaaa tcaagtattt
     4861 ccagcaagct atgggagaaa aaaaagacaa acaggaagga ggtgagagcg ataatgacat
     4921 aggcttgttt tttcatcctc taaccacctc cacatcacca atcatagtgg tcaagaaaat
     4981 cttgacactc ttgttactct tgagatagtc ttttgtttct tgatgatagt gttcattgcg
     5041 gagggctcgc ttgggctggt tgaaaaaagt caaatcccca tagagacagt taacgctgag
     5101 actgacttcc acatctacag gtacgatgat tttggtcgtt cctaccatct ttctgaggat
     5161 aatgacattg tcatgattgg ttaagatgac cctctccaga tgaatagtgt ccttgcccat
     5221 gaagcgaaag agattgatat catcgaattg gcaagtctgg tagcttgaaa aatgatgaag
     5281 atttccaaac caacgatttt tctccttctt aaccgtcacg acctcttcaa aaaccaaatt
     5341 ggtctgctct ttttcctggt tcatcatcgg gtaaagaaga aagaggctat agataaccgc
     5401 aacaaaaata gctagaatca caaaaggatt gagcataacg atgaaaaaga agagaatggt
     5461 tgccgctact aaaagaagat tatttccctc tttaccagtg tagtagcgaa tcaaaagcaa
     5521 aaagaggaat agtatcagca gaaaacgcga aaaatgctct gataccatca aaatcagagc
     5581 tcctgtcaga agacaggctt cgataaataa aaagatttta aattttctca taggttcatc
     5641 ctctcccttc tattttatca caattcaaaa aagtcacctc agtctgagga tggaaaaaaa
     5701 ggcgctggtt acgccttttt catctgatca tttgcttctt ttaattttcc ataaagaaga
     5761 tagtctactt tttgtagatc tgctatggtg gcacagttaa gggaacacat aatcaagcgt
     5821 agatctgctt tccagccttg gacaatgcca atcacttctt caactgtgta ggtttcaacc
     5881 aattccagaa cggttcgtga caatcccaca gccttagcac caaaaaccaa gcacttaatc
     5941 atatccagcg gattccgaac ccctccacta accaagagtt cgaccttatc tttccattct
     6001 tgggcattga gaagggcctg catggtagac tgaccccatt gattgaggta atcacgctgg
     6061 ccactacgac ggttttcgat ataggcaaag ctggtgccac cacgacccga taggtccact
     6121 gtacgaacac cgaattcata ggctctttcg attgtcttgg catccattcc aaagcccact
     6181 tccttgagga caataggaac gggaatttgc ttgctataat ctgctagatg cgattgccag
     6241 cttctaaact tcctttctcc ctcgggcatg agtaattcct gcatgacatt gacatgcact
     6301 tgcaatagaa caggattcat ctcttctaca gtctgaagtc ctaactctac aggcttgtcc
     6361 aatccaatat tggttccaag gaggagattg ggatgactag acttgacaga aaaagaatca
     6421 tccgttggat ttttgagggc tgcgctataa gaacccgtta caaataaaat gccacaggat
     6481 tccgccacct gagccagctt ttgattgatt tctcttccct tattacttcc accagtcatg
     6541 gcattgatat aaaaaggaaa gtcccacttt cgaccagcaa actctgtcga cagatcgatt
     6601 tcatccagat tgtaaagagg caaggaagaa tgaatcagct ccacctcatc aaagctatta
     6661 taggaacttt tctgctcaag ggcatagagg atatgctcgt ccttacgatt tgtcgtcatg
     6721 tcctatcctt tcttgatata agagctcaat ccccagatcg gcccaacgat tttttaaggt
     6781 tttggttgat tgcgcatcaa aactcaaggc gatgccacag tcaccaccac cagcaccact
     6841 actcttggca acggcctgca aatcttgact ggcttctttc aactgtctaa gcaaaggcgt
     6901 gtaaatatct gtactcaagc cttctaaaag cttgctggct acttctactt gctcgataac
     6961 tttttcggct ttcccctgct ccaaggcttc gaccaaagaa accaccgttt cttttgagga
     7021 agttaaaaaa ttttgattga tattttgctt gatttgctgg accatgtgac tcgatacagc
     7081 cacttccttg gtccatccca ctaagaaatc acattctaaa gttggtttca cttgtgaaat
     7141 tgaaaagccc caatcacgct ccagaactgt cgccaagttt tcttcttcta accaagcagc
     7201 caccttctgg cgatcaaatg actggtagag aaccaaatcc tctgccacaa tacaggcaag
     7261 gtcccccatg gaaccattgt ctcctcgctt gagcaagaca gcgctagtca gcttgaacaa
     7321 gagctcctga tcaacagaaa catcatacag agccagtaaa gccttgacaa ccaagacaac
     7381 gacgctgcca ctagaaccta gaccaaactt tttcccttct cgttccattt tgccacagat
     7441 ttctagagaa aaaggtctta aattctgacc acgaacagcg aggaagtctc ccatcaaaac
     7501 aatcgtttct tgaatcaagc tatagtcagg attgggcctt aagtccactg cgaaatcaaa
     7561 catatctgaa tagatacggt agctatcaga aaaagcaatc tcagccctca tatagatggg
     7621 aatatccttt atcaaagcta actgccctgg ctctaaaata gcatattcac ctgccaaata
     7681 gagttttccg caagttttaa cagcaatcat cttgactcaa atcctttgtt tttgacacaa
     7741 tcaagcgata acgctgaccg aaaatttctg acaaatgctc caagtctttc tcctgacaga
     7801 agaccttaac attgggacca gcatccatgg taaagtagca ggcctctcct ttctcacgaa
     7861 gctggcgaac aaaggccata gcctcataag aggcatccgt cagataagaa aaggctggac
     7921 tagcagtctt tgtcgtagca tgcatagcca gagcattttt ctccgttaat tctccaatct
     7981 tggcaaaatc attttccttg agataaatca gcatatcctg atagtccttc tcagactgac
     8041 gaacccagtc gtcaaaagtc gtcgaggttt ccacacaaag tttcatccca tcacggctag
     8101 agattggttt tttcttgtcc tctagcacca acataatcat agctagtttc aagtctgtct
     8161 ctacagggta aatttctcca ctatccttat cccaggctcc tagtggtcca taaaaactcc
     8221 gagaagaaga gcctgaggca aacttggctt cctgcgctaa ctgacttcta tccaatccaa
     8281 gcttgaaata agcattacaa gccttgacca gggcggacaa accactagaa cttgaggaca
     8341 ggcccgctgc cgtaggcata ttgttttgag tatcgatacg gacaaagccc tcaccagctg
     8401 gacgataacg gtcaataatc ttactcatct tggcatgctc gacctcattt tgtagctgac
     8461 cattgatgta aaattcgtca gctgttacat tggctggtaa aggcgacaag gtcgtctctg
     8521 tatacatatt ttccaaagtt agagaaatac tgctagtagc aggcaccatc tctttttctt
     8581 ttttctttcc ccaatatttg ataatagcaa tatttgcgta ggaacgtact gtcacaggct
     8641 ctctatccat gtctgaacag ctcctttctc ttctaatctt tctgctagtt cttgtgcgtg
     8701 tgtcaaattg gttaccaagg ctatgataca acctcctagc ccaccaccgc tcatcttggc
     8761 acccagagca ccatggctaa gagtcgtttc aaccaaaaag tctgcctcag ggctactgac
     8821 tccaatttct tttaaatgta aatgcgcttg actgaggatt tgtcccagtc cttcagcatc
     8881 tttttgtgaa atcgcaactt ctgcttgctg ggttaattct cccaaggcat gcaaaaacgg
     8941 tagggcatcc ttgcccttat tttgaaccac ttggatggct tcacgagtat gaccataaac
     9001 acccgtatcg gcaatcacca aataggcgga taaatccatc tcaagttctg taaatcctac
     9061 gttcttgata aagcgaatag gttggtcact gagacaggtc ttagcatcca aaccactagg
     9121 attcatatgg gcaatcatct cagctcgatt gaccaagatt tctagtacat catgaggcag
     9181 atcagcctga tagtagtcaa ataccgcacg aatggccgct atgctgatag ccgctgacga
     9241 acccatcccc cgtttctcag ggatagccga gtcaatctca caacgaatgc aggcttctgt
     9301 gatatccaaa tactccagtg aggcataaac cgccatggac aaggtatcct cctcataaag
     9361 gcgccaagga ctctctgcag gaactacctt acaggtcacc tccacctcca aaagaggcag
     9421 ggaaatggca ggataaccgt aaacgaccgc atgttcccct attaaaatta tcttactatg
     9481 tgcctgaccg acaccaactt tttttgtcat tttttccttt tactagacga aaaaacgtct
     9541 tatttttcat acaagtatta attctttcct atctatttta ttatattttc acaaaaaaag
     9601 cgattgtttc cattcacaat cgcttctttc attattgaac ccattcgcca ttatagttga
     9661 cagaatagcc atctacggtc gtattcactg ccaaggcacc tgagctataa gcgtagtacc
     9721 atctgccatt gacctggaac caacctgtcg tcatagaacg acgaaagaaa ctccataccg
     9781 ttaagtaaag aggaaagtcg tgagggatca tgcgccattg acaacctgtt ttagtgacgt
     9841 acaaagtctc attaacaagt actcgtttcg gccatttata ggtgcggtgt ttggagaaat
     9901 agggttcaat cttagcccat tcttgatcgt ttaaatcagt atcatatgct ttgcgtgtca
     9961 taactctagt ttaacatttt ttgtgaatac aagttctaaa taatcgacca cgaaaatttc
    10021 ttaagtggaa aacgccttat gaagtatgct acgggaaact tatgcactta atttgacaat
    10081 tcaagatgta aaaatatata
//
LOCUS       DAVBGY010000064         9575 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVBGY010000064 DAVBGY010000000
VERSION     DAVBGY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9575
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.064"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            360..947
                     /locus_tag="VQ620_001762"
     CDS             360..947
                     /locus_tag="VQ620_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764345.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGTLLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQKKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            complement(1034..1237)
                     /locus_tag="VQ620_001763"
     CDS             complement(1034..1237)
                     /locus_tag="VQ620_001763"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764346.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(1534..2238)
                     /locus_tag="VQ620_001764"
     CDS             complement(1534..2238)
                     /locus_tag="VQ620_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET2764347.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            complement(2308..4134)
                     /gene="glpO"
                     /locus_tag="VQ620_001765"
     CDS             complement(2308..4134)
                     /gene="glpO"
                     /locus_tag="VQ620_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HET2764348.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPILDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            complement(4176..5684)
                     /gene="glpK"
                     /locus_tag="VQ620_001766"
     CDS             complement(4176..5684)
                     /gene="glpK"
                     /locus_tag="VQ620_001766"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET2764349.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            complement(5843..7270)
                     /locus_tag="VQ620_001767"
     CDS             complement(5843..7270)
                     /locus_tag="VQ620_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2764350.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLDSAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            7435..8307
                     /gene="hslO"
                     /locus_tag="VQ620_001768"
     CDS             7435..8307
                     /gene="hslO"
                     /locus_tag="VQ620_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET2764351.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQERHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGAGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            8294..9274
                     /gene="dusB"
                     /locus_tag="VQ620_001769"
     CDS             8294..9274
                     /gene="dusB"
                     /locus_tag="VQ620_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET2764352.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
BASE COUNT         2728 a         2175 c         1914 g         2758 t
ORIGIN      
        1 acgtctattt attttttatt gttttttatt gtagagcaga cccaagcgaa tgaacatagg
       61 ttgtaaaaag caaatgaaaa aactgcgagg ataatagctt ctgtgtctaa ataacccatt
      121 tcgagacctc ttttcaacaa caacaagcct gtagtttcga taacgatcaa acataaaaat
      181 atataaaaaa ttttcgcatt aagcatattg ttaccgcttt ctttttattt ggttttattg
      241 tataataaaa gttgaaaaaa atcaaaaata caaaggagtt tccgttatgt ctaagatgtt
      301 taaatgcatg tgtttgctag ttatgtctgt tttctgtatg tctagcattt ccccgtcgct
      361 tgtttgtttt tgctgatgat agcttatcgg caaactctaa agttgtatct ggtgaggctc
      421 agtttgagaa tggctcatca gtgcggttcg gagatacgca agtaaatatt ttaagcgatg
      481 aagttcttga ggttgtcaat ccagatggaa gtgtagacac tattgaacga cgtgctgacg
      541 gcgtctatat caatggggca ttttatatgg cttatcaaaa gaatgaaatc gacctaaata
      601 tatcttttag atcatacgat cctaatgttt ggaattacgt caacacaata catgggaata
      661 aacaagctaa cacattcgct aacttcatga ctggtgctgg aatcagttat atgattggta
      721 ggataggtac tttattgggg ggtccatggg gtgcaataat tggtggagca tattttggta
      781 tacaagctta tcagtcttac ttggattcac aaagcccata tccatactac ataacaagta
      841 cttatatcca tgttgctcaa aaaaaatgga agtttatcac tgaatactat agaaactcaa
      901 actatactgg gtatgtaaaa accgtaacaa cctacgtgaa tttctaaaat ataaaccctg
      961 cctagatata aaggcagggt ttatatttta gttaattgct caagtaattt gtaagcataa
     1021 tacttgcgaa actctatttg ttcaagcggt tcaaaagctc ttgtttttta tcctcaagct
     1081 cagctctcct ttgctcgatt tcttcacgtt tttgggcgat ttctgcttgc gcctcgggag
     1141 tttccaaagt gccttgtgac ttgtgaaatt ctactagcat atcaacgatt tttgggtcta
     1201 attcgttgac ataatcagga tttgaccatt tcggcacgtt gcttgcaggt gcttgctttt
     1261 tgccgccttt tttgggcgtt ttggcttgtt gttcagcttt ctgactgtag ttgacctcgt
     1321 ggaattttcg tgcttgatat gatgcgtttt attatgagaa gatttacttc attttttgct
     1381 gaaattgggc tcttgcccaa gctctatcaa cataattgtt ttctaatact caatgaaaat
     1441 caaagagcaa actaggaagc tagccgtaag ctgtacttga gtacggtaag gcgacgctga
     1501 cgtggtttga atttgatttt cgaagagtat aaactagaaa agtgagaata caagcactgc
     1561 caaggctgct ccgataacag ggcctacaac aggaatccaa gcgtaagacc agtctccgtc
     1621 tcccttgttt ggaattggca agatgctgtg catgatacga ggtccaaggt cacgagctgg
     1681 gttcaaggca taacctgttg tcccacctag tgatagaccg ataccgacaa tcaaagttcc
     1741 cactgcaaag gttccgatac ctgcctgaaa atcgtaaaga cccaaagcaa agattgtcaa
     1801 caccaaaaca aaggttccaa ggatttcgct aatcaagttt gatacagtat ccttgatggc
     1861 tggtccagta ctgaaggttg ccaggatatt gcctgcattt tcttctgcct catagtgagg
     1921 tttgaattgc aaccaaacca aaatctgacc cagcatggcc cctgcgaact gggctaagat
     1981 ataaggcaaa acggaagccc aaggcaaacc accttttaag gccacaccga tggtcacagc
     2041 tgggtttaaa tgagctggac tgagcttgcc agatacaaag actgcaaccg caactgcaat
     2101 cccccaaccc atagtaatca caatccaacc tgagctattg ctcttggttt taggaagaac
     2161 cacacctgca acaacaccat ttcctagaag aatcaggatt aaagtcccta gaaattctcc
     2221 aaataattca ttcatcatct ttctgtctcc attaaaaaga agggacgggc gacaaggaat
     2281 gctgccctcc acctctttta ttttttctta attttttaat tctgctaaat cgttgttagc
     2341 gagagctgct tcgacatcag cacggtaagt tgctttttct tcttctgtcc agtcatagaa
     2401 tcgtcccatt tcatccaaaa ttggctcaac gatgctatcc aagctatcac gcataaagag
     2461 catatggttg gtacgacgaa gaaggaagtc aactgggcta agagccaact cgttgcgcat
     2521 tgcatagtga agggacaaag tatctgccaa gctgagtcct ggcgcttgtt ccaagctgtg
     2581 agcaagtgca aagactttcg gtgcatttga accgtaaaga tttgccagat agtgagcttc
     2641 cttgctatcc aaaccacgtg atactccaag ttgcgcaaag gcttcgattt ctgaatccac
     2701 atttgctggg ttcaattctc cacctgaaac agggtaagtt ttagaattga tcaatttaaa
     2761 gctacggtca aattctgctt tgaggatgtc aaccacgcgc tccatagctc cttcagccat
     2821 cttacggtag tctgtgattt taccgccagc aagagtcaaa agaccattgt catcacggtc
     2881 caagctagac ccacgagaaa ctgcagatgg atccaaatgt ttctcagatg tgctactttc
     2941 aagcttgctg acagcagact caacatcttc acgtgttttt tctttggaga gataagattc
     3001 aacagtcgca atcaagttgt caaagctttc atcactgatg gtaccgttat ttccaccatt
     3061 atagtcagag gcactgttcc ctgcaatcaa tggacgaaga cctgcccagc tgctttcgat
     3121 atcatcaatg gtgatgttgg attctggaaa gcggttgttg acaatgccaa gtagataatc
     3181 tacatcttct tgagttactt ttggatgctc caaatcacct gtgtagtctg tatcagttgt
     3241 accaaagtaa gtcttgtttt cacgtgggag aacaaagacc atacgaccgt cacccaaacc
     3301 tgtgtcaaag taaactggct gtgaaacctt gattttgctt gaatccacta ccaagtgaac
     3361 tcccttagtt gggcgcattt gtgagaattg cgttccctta ttagacaaat tacgtacttt
     3421 atcactccaa ggacctgttg tattaataac cagacgggcc ttgatttcaa atacttggtc
     3481 tgtcaagaga tcacgagcta caacacctgt aatcttgcca ctttcgtcaa agaggaagcc
     3541 ttctgccttc acgtggttgg caatgagggc accgtcttgg ttggcacgtt tgatgttttc
     3601 aatcacgaga cgcgcatcgt tgttacggaa gtcaagatac actccacctc ctaccaagcc
     3661 ttccttcttc aagtttggct ggcgttccaa gacttgatcc ttgctcaaaa ccttgttcgc
     3721 agttggtgtg ttgctaacac ctgccaagag gtcgtacaag tccatggcta ctttaagacg
     3781 gaagaggcta aaggttgctc catcttcatc gtaaactggt aagagcattg ggtctggttt
     3841 tggaatgtgt ggagcgattt gttgaaccac tgcacgttca gaaaccgtat ctgagaccac
     3901 ttctacgtcg aattgtttaa ggtaacgaag tcctccgtga accaattttg ttgaacgact
     3961 agatgttcct tctgcaaagt cttgcatttc aatcaaacca gtctcaagac cgctagctgc
     4021 cgcctgcaag gctacaccag ctcctgtgat tcctccaccg ataatcaaga ggtccagggt
     4081 gcgttcctgc atttttttaa ttgacaattc acgtgttttc tttgaaaatt ccatatttac
     4141 acactttcta aataaatcgc tttattctgc cagtattagt cgtctacttc cgcaaagact
     4201 tgagttgctt tcacagcctt cttccagccc ttgtagagtt gttccttgcg agattcgttc
     4261 atagatggct caaagagttc tcctgtctcg ttcaagagtt tcaactcgtc caagtctttc
     4321 cagtacccta ctgacaaacc tgctaggaag gccgctccta gagctgttgt ttccaggttt
     4381 ttagcacgtg caatgtcaat gcctaaaata tccgcctgga actgcatgag gaagttgttc
     4441 atggctgcac caccatccac cttcagtact tgaatggcgg tctgagtatc cacttgcatg
     4501 gtgtcgatga tatcacgcac ttgataagca atagattgca aagtcgcctt gataaagtct
     4561 tctttgcttg ttccacgagt caaaccaaag acggaaccac gagcattttg gttccagtat
     4621 ggagcgccta gacctgtaaa ggctggaacg acataaactt catcgttgtt gtgagaatca
     4681 cgagcgtatt tttcagattc tggtgaattt tcaaccatgc gaagaccgtc acgaagccac
     4741 tgaatagcac ttcctgcgat gaagatagaa ccttccaagg cataataaac cttaccgttg
     4801 attccgtaac caatggttgt caagaggttg ttttcagaca actgcatctc ttccccagta
     4861 ttcatgatga tgaaagagcc tgttccataa gtattcttaa ccatacctgg ctcaaaagcc
     4921 aactgtccaa agagggctgc ttgttggtcc ccagccatac ctgagattgg cacctctcca
     4981 ccgtagaaat ggaatggagc tgtcttgccg tagatttcgg agttagaacg aacttctgga
     5041 agtatagcct tcggaatgtt aaggatttcc aaaatctcat catcccattt gagttcttta
     5101 atgttataaa gcatggtacg agctgcattt gagtagtcag tcacgtgagc cgcaccgtca
     5161 gtcaatttcc aaaccaacca agtatcgata gtaccaaaga gcaattcccc tttttctgct
     5221 cgctcttgag caccttctac atgatccaaa atccaacgaa ccttggtagc agagaagtaa
     5281 gcatcaataa tcaaaccagt cttttcatgg aatttttcca cataaccttg gctttttagt
     5341 tgctcagcca aaggtgctgt ctggcgtgac tgccaaacga tagcattgta gataggaagt
     5401 cctgttttct tatcccagac aaccgttgtt tcacgttggt tggtaatccc gattgcctcg
     5461 atttgatttg gcttgacacc actttcgatg aaagcacccg caataactga ctgaacagag
     5521 ttccaaattt cattggcatt gtgctcaacc caacctgcct gagggaaaat ctgggtaaac
     5581 tctttttgac tcgagctaac cttttcccct tttttgttga aaatgatggc acgagaactt
     5641 gtagttccct ggtcaatggc catgatgtat ttttcttgtg acatgtactg tcctcctgat
     5701 aaagatcttg aggtgtttcc tctttacact tactagtata caaaataaag cgctttcttg
     5761 tttatcaact ttttttaatt tttaaaaaaa tgtgaggaga ttgtgtgaat ttcacaaaaa
     5821 agacctagat taaccaagcc ttttaagtga ataataactg acgaatccct gccaaatctt
     5881 ccatatccaa gtcgtttttt aaataataga ctggtgtctg ttccttctta tgaatcgggt
     5941 tattggtaac cagcaaatca taatgccgac tagggtcata agcttcaatg gtaataaaac
     6001 gattgtattc caaataccgt ttcaaaatgg ctgccatcct tgaaaagaca agaaaaccag
     6061 atgtcaaatc cagaccaatc cgcatatgct ggccaccgtt ttcagccata tattcgatta
     6121 actggagcca ttcccagaga atcttcttat ctaaatctgt cccctgttga aaagcaggta
     6181 gagcatgaaa aatctcctct gctgtctctt taaaatcatg ttccatcagt aaatatggat
     6241 gacgattctc taactggtac ttgtagcgat cctgtaaaat atagccctta tagagataga
     6301 cttgagcaca aagctgactg agttcatagg tgacatggtc ctctgtataa tcccccaata
     6361 gttcctcctt cttcatttct tgaatcaatt gcgtcagtaa atctgccaac tgccctccaa
     6421 aaccaagaat atactccata gtatgaagag gaagaatgcg atgagatagg agaaaagaga
     6481 agaatatcat catctcacca tcctcaactc ctagtggaat atgttgccca gcaaaaaagg
     6541 acggaacctt tctcagcaaa cgaagataaa agatattgtc aaaataccct cgcattttct
     6601 cttctatgac ttgaaactga caagcattga cccgaagacg ttgttgactg atgtgagccc
     6661 agagaaccaa gtccaatttc tgccccgcag acaaacttgc accgcagatt tcctctaaat
     6721 tggcaatctc ctgttttctc tctggtttct gcatgtgacc ttcccattcc tgactcgacc
     6781 agacctttcg gaaaagacag aaatagaaat agcgaatctg atgctctgga cctctccagc
     6841 gtccattttg gatggataaa tcaaattctg acaaaatctg atttaaacca gccaagtgac
     6901 gaccaagcgt agcctcgcta atcaccaatt cttgagccag ctgatgggct aaaaactgtt
     6961 ggtggtagag aagataaacc aaaatctggt atttaacagc actatccaaa aacaagctcc
     7021 gaatatctct ccccttggta gctgcaccga tagacagacg cagattttca tcttctgagt
     7081 gaatagtcag ttctaagcca ctatccaaag ccttgtcatt gagcagggtg acatatttgg
     7141 ttagggttgc ttttgaaaat cctgtttctt ccattacagc cttgacggtc gtctgagact
     7201 cttgtaatag aaaggaaagg attgaaaatt gaccacactc ggctttctcc atcaaatctc
     7261 ctaaatacat ttgttacccc tttcattttc taccttaagc atagcataaa tcttacaaat
     7321 gctgaaataa tctgtttctc tttttatttt catgctgatt tcctggtcca ttatcctgaa
     7381 aatcagtaaa cacacggctc cccctttggg catttttatg ctaaaatagt agctatggat
     7441 aaaattatta aaactatatc agaaagcgga gcctttcgtg cttttgtcct tgatagcacg
     7501 gaaaccgtcc gcactgctca agaaagacat caaacccaag ctagctcaac tgtagcgctt
     7561 ggtcgaactc ttatcgctag ccagattctc gcagccaatg aaaaaggaaa taccaaactt
     7621 acagttaagg tgttgggctc tagctctcta ggtgctatta tcaccgtcgc tgataccaag
     7681 gggaacgtca aaggctatgt tcaaaatcct ggtgttgaca tcaaaaagac tgcgactggt
     7741 gaagtcctag tcggaccttt tgttggaaat ggtcaattcc tcgttatcac agactacggt
     7801 gctggaaatc cttacaactc tataactccc ctcatctctg gagaaatcgg tgaagacctt
     7861 gccttttacc ttactgaaag ccaacaaacg ccttcagcgg tcggcctcaa tgtccttttg
     7921 gacgaggaag acaaggtcaa ggttgcaggt ggtttcctag ttcaagtctt gccaggagcc
     7981 aagaaagaag agattgctcg ctttgaaaaa cgcatccaag aaatgccagc tatctctact
     8041 cttctcgaaa gcgacgacca tatcgaagcc ctcctcaagg ctatctacgg ggacgaagcc
     8101 tacaagcgtc tttctgaaga agaaatccgt ttccaatgtg actgtagcca tgaacgcttt
     8161 atgaacgctc ttgccagcct tccaagctca gacttacagg aaatgaaaga ggaagaccac
     8221 ggggcagaaa tcacttgtca attctgccaa actacttaca actttgatga aaaggacctg
     8281 gaggaactca ttcgtgacaa atcttaatac accttttatg attggcaata ttgagattcc
     8341 caatcgtacc gttttagcgc ctatggctgg cgtgaccaac tcagcctttc gtaccatcgc
     8401 aaaagagctc ggagctggac tcgttgtaat ggaaatggtc tctgacaagg gaatccaata
     8461 caacaacgaa aaaaccctgc acatgcttca tatcgatgag ggcgaaaacc ctgtctctat
     8521 ccaacttttt ggtagcgatg aagacagcct agcacgcgca gcagaattca tccaagaaaa
     8581 caccaagacc gatatcgtcg atatcaacat gggctgccct gtcaacaaaa tcgtgaagaa
     8641 cgaagctgga gctatgtggc tcaaggatcc tgacaagatc tactctatca tcaacaaggt
     8701 ccagtctgtc cttgatatcc cacttactgt caaaatgcgt accggatggg cggacccatc
     8761 tcttgcagta gaaaatgccc tcgctgctga ggctgcaggt gtttctgccc tcgccatgca
     8821 tggccgtacc cgtgaacaaa tgtatactgg ccacgcagac cttgagaccc tttacaaggt
     8881 tgcccaagct ctaaccaaga ttccattcat cgccaacggt gatatccgta ctgtccaaga
     8941 agccaagcaa cgcatcgaag aagttggtgc tgacgcagtc atgattggcc gagctgccat
     9001 gggaaatcct tacctcttca accaaatcaa ccattacttt gaaacaggag aaatcctacc
     9061 tgatttgacc tttgaagaca agatgaagat cgcctacgaa cacttgaaac gattgattaa
     9121 cctcaaagga gaaaacgtcg cagttcgtga attccgcggc ctcgctcctc actatctccg
     9181 tggaacatct ggcgctgcca aactccgtgg agccatttcg caagctagca ccctggcaga
     9241 gattgaagcc ctcttgcaat tggagaaggc ttaatagttt aaaacccgta actctcttaa
     9301 agagcctctt gaatgccgcc attctttcct catcaaaaaa actccgtagt ctctagcgct
     9361 ataaatccgg ctagagatta cagagttatt tttttatcta aacttcctac aagggtatat
     9421 tatctactga aaggatatgg tatttgtagg gaatacttta aagagttctt acaggaagtc
     9481 agtattaact agttatatta ggtttagttt acccattcac cattggcatt gactctatag
     9541 ctatctacag ttgtgttgac tgcaagggca cctga
//
LOCUS       DAVBGY010000065         9388 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVBGY010000065 DAVBGY010000000
VERSION     DAVBGY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9388
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.065"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(196..654)
                     /locus_tag="VQ620_001770"
     CDS             complement(196..654)
                     /locus_tag="VQ620_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HET2764353.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(651..1091)
                     /locus_tag="VQ620_001771"
     CDS             complement(651..1091)
                     /locus_tag="VQ620_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2764354.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1447..3396
                     /gene="mutL"
                     /locus_tag="VQ620_001772"
     CDS             1447..3396
                     /gene="mutL"
                     /locus_tag="VQ620_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET2764355.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(3721..4188)
                     /locus_tag="VQ620_001773"
     CDS             complement(3721..4188)
                     /locus_tag="VQ620_001773"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET2764356.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(4189..5394)
                     /locus_tag="VQ620_001774"
     CDS             complement(4189..5394)
                     /locus_tag="VQ620_001774"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET2764357.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(5414..6049)
                     /gene="ribE"
                     /locus_tag="VQ620_001775"
     CDS             complement(5414..6049)
                     /gene="ribE"
                     /locus_tag="VQ620_001775"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET2764358.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(6034..7134)
                     /gene="ribD"
                     /locus_tag="VQ620_001776"
     CDS             complement(6034..7134)
                     /gene="ribD"
                     /locus_tag="VQ620_001776"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET2764359.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGNLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(7269..7383)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7540..8133
                     /gene="ruvA"
                     /locus_tag="VQ620_001777"
     CDS             7540..8133
                     /gene="ruvA"
                     /locus_tag="VQ620_001777"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET2764360.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            8143..8706
                     /locus_tag="VQ620_001778"
     CDS             8143..8706
                     /locus_tag="VQ620_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET2764361.1"
                     /translation="MTKRCSWVKMTNPLYITYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            8703..9380
                     /locus_tag="VQ620_001779"
     CDS             8703..9380
                     /locus_tag="VQ620_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2764362.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSVIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVRL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
BASE COUNT         2651 a         1843 c         1863 g         3031 t
ORIGIN      
        1 aaatctgaaa taactgttat gatatagcag attgaaacta gaatagtaca cttttgcttc
       61 taaaacattg ttagaaatcg atttgactgt cctgattgat ttatcctgtt cttatttcat
      121 ttcactatag taaggtgcag caggataaag caaaaccgag aagtttcttt ctcggttttg
      181 cttattatga ggttcctatt ttctatgctc caagagttga ttgatgtgat ctactttttt
      241 agcctctctt ttatagagaa tagcccagat gatgagatag acaatcgcaa actcgataat
      301 gagctggagg tagaaaatcc agtggaaggg gaaccaacca gcaagagttg ctagtgggac
      361 aaagccagcc agcataagga agaaatgagt cagagtggca cgaagcatgc tccagtcacg
      421 gctgaataag cggttgccaa agttgaagag cataccgatg gctgcccaga taagtgtgca
      481 gtagagcaag accagggcac cgtgaacctg atgctgatcc atcacttggc cgatgagaga
      541 gtagggattt agtggtgcgt aggtatttgg tgcataaatg agtgaaaaga ggatggagag
      601 gatgaggccg ataagaacac cggcagctgc atcgtgaaat acttgttttt tcatagttct
      661 aatttctcct tgatggtttt tagataacgg cgtgaagagt aggtgaagct ttcgtttttc
      721 aagaaaattt ctaccagacc gtttggcgtg agcttgaggt gagagatgga atcgatattg
      781 atgatttctg attgggaaat ttggataaaa ttggttggca agagtttaag aacctgatag
      841 agtcgcaaat caatgctgta ggtctgactc gcggtttctg ctagaacctt ccgattctcg
      901 atatagaagc gctgaatctt gccaatctca actagataga cctgatcatc gatttttcct
      961 ttgatttttt ctctttggtc cagattttct gcgaactcga tgactttctg gactttttcg
     1021 gtttcttgag gtgcttggac aatcagcttt tcctcctcgt aagtctcact aatctgtagt
     1081 tctactttca taaatttctc tccttgtttt tcatacaaaa ttaatctcac ttcttgtaca
     1141 cctttattaa tactcttcaa aaatctcttc aaaccacgtc agcgtcgtct tgccgtagga
     1201 tagttgttga ctttgtcagt tttatctaca acctcaaaac agtgttttga gctgtctgcg
     1261 tctagctttc tagtttgctc tttgattttt attgagtata ccactatttt actccctctg
     1321 gcaagggact ttgtctatgt ggagggattg ggctcctatg tggtggagtt tttctgttct
     1381 ttctgaaata tggtataata gcactaatta atttctagga aaatagatac agaaaggggc
     1441 tgaaagatgt ctcatattat tgaattgcca gagatgctgg caaaccaaat cgcagctggg
     1501 gaggtcattg aacgtcctgc cagtgtggtt aaagagttgg tagaaaatgc cattgacgcg
     1561 ggctctagtc agattatcat tgagattgag gaagctggtc tcaagaaggt tcaaatcacg
     1621 gataacggtc atggaattgc ccacgatgag gtggagttgg ccctgcgtcg ccatgcgacc
     1681 agtaagataa aaaatcaagc agatctcttt cggattcgga cgcttggttt tcgtggtgaa
     1741 gccttgcctt ctattgcgtc tgttagtgtc ttgactctgt taacggcggt ggatggtgct
     1801 agtcatggaa ccaagttagt cgcgcgtggg ggtgaagttg aggaagtcat cccagcgact
     1861 agtcctgtgg gaaccaaggt ttgtgtggag gatctctttt tcaacacgcc tgcccgtctc
     1921 aagtatatga agagccagca agcggagttg tctcatatca ttgatattgt caaccgtctg
     1981 ggcttggccc atcctgagat ttcttttagc ttgattagtg atggcaagga aatgacgcgg
     2041 acagcaggga ctggtcaatt gcgccaagca atcgcaggga tttacggttt ggtcagtgcc
     2101 aagaagatga ttgaaattga gaactctgac ctagatttcg aaatttcagg ttttgtgtct
     2161 ttacctgagt tgactcgggc taaccgcaat tatatcagcc tcttcatcaa tggccgttat
     2221 atcaagaact tcctgctcaa tcgtgctatt ttggatggtt tcggaagcaa gcttatggtt
     2281 ggacgttttc cactggctgt cattcacatc catatcgacc cttatctagc ggatgtcaat
     2341 gtgcatccaa ctaagcaaga ggtgcggatt tccaaggaaa aagaactgat gaccctggtt
     2401 tcagaagcta ttgcaaatag tctcaaggaa caaaccttga ttccagatgc cttggaaaat
     2461 cttgccaaat cgaccgtgcg caatcgtgag aaggtggagc aaactattct cccactcaaa
     2521 gaaaatacgc tctactatga gaaaactgag ccgtcaagac ctagtcaaac tgaagtagct
     2581 gattatcagg tagaattgac tgatgaaggg caggatttga ccctgtttgc caaggaaacc
     2641 ttggaccgat tgaccaagcc agcaaaactg cattttgcag agagaaagcc tgctaactac
     2701 gaccagctag accatccaga gttagatctt gctagcatcg ataaggctta tgacaaactg
     2761 gagcgagaag aagcatccag cttcccagag ttggagtttt tcggacaaat gcacgggact
     2821 tatctctttg cccaagggcg agatggactt tacatcatag atcagcacgc tgctcaggaa
     2881 cgggtcaagt acgaggagta ccgtgaaagc attggcaatg ttgaccagag tcagcagcaa
     2941 ctcctagtgc cctatatctt tgaatttcct gcggatgatg ccctgcgtct caaggaaaga
     3001 atgcctctct tagaggaagt gggcgtcttt ctagcagagt acggagaaaa tcaatttatt
     3061 ctacgtgaac atcctatttg gatggcagaa gaagagattg aatcaggcat ctatgagatg
     3121 tgcgacatgc tccttttgac caaggaagtt tctatcaaga aataccgagc agagctggct
     3181 atcatgatgt cttgcaagcg atctatcaag gccaatcatc gtattgatga tcattcagct
     3241 agacaactcc tctatcagct ttctcaatgt gacaatccct ataactgtcc tcacggacgt
     3301 cctgttttgg tgcattttac caagtcggat atggaaaaga tgttccgacg tattcaggaa
     3361 aatcacacca gtctccgtga gttggggaaa tattaaaagt ataaaaaagt ctgggaaaaa
     3421 ttttcaaaat caaaaaaacg cataaaatca ggtgttcaaa aaccttgatt ttatgcgttt
     3481 tatcatggaa atagttactt cattttttcc taattctttt cgaaactctt tttaaaccac
     3541 gtcagtttta tcagtaatct caaaacagtg ttttgagcta attttgccag ttttgtctgt
     3601 aacatcgaag ttgtgtttga ccactctgcg actggtttcc tagtttgctc tatgattttc
     3661 acagagtatt aaattgcgat tttgccaagt ttttttattc gtctaaaagt agagtctgtt
     3721 ctatgcgtct aatgtacgaa ttaggttgac catttcaata gctccttgtg cacactcaga
     3781 acccttattt cctgctttag tgccagctcg ttctatggct tgttcaattg tatctgtcgt
     3841 tagcacacca aacataacag gaatttcgct atttaaactg atttgggcga ttcccttaga
     3901 tacctcgcta catacataat cataatgact tgtattccct ctaatgacag ctcccaagca
     3961 gataattgca tcatattttt tactttttgc catttttgat gcaatcagtg gtatttcaaa
     4021 agctcctgga acccaggcta cctctatatc tttctcgttt acattctctc ttttgagatt
     4081 atctagtgct ccagataata attttgaagt tataaattca ttaaatctcg ctacaacaat
     4141 acctatttta atattgtttg ctactaaatt accttcataa gtgttcattt atttttcctc
     4201 catatttaaa atgtgaccca ttcgattttt ctttgtttct aaataaaaac tatcgtaagg
     4261 attggcttct atttcgattg atattctact ggaaatggta attccatatt tttctaactg
     4321 ttcaaccttg tcaggattat ttgtcagtaa atgaagtgac tgaagtccca gatctttaag
     4381 catttgtgct ccaatatgat attctcttaa atcaccttca aagcctaatg caagattggc
     4441 atcaagcgta tccatgcctt gatcttgtaa atgataggct tttaatttat tgataagtcc
     4501 aattcctcgt ccctcctgtc gcaagtaaag taagacaccc gaaccattct caacaatcat
     4561 tttcatagct ttatcgaatt gctgtccaca atcgcaacgt aaagagccta aaacatctcc
     4621 tgttaaacat tcggagtgga cccgacataa tacattggct tcatcctcta tgtttcccat
     4681 aataagagca agatgatgtt ccccatttag tttatctata tagctaattg ctttgaaatt
     4741 accgtatcta gtaggcatat tgacagttga aactcgttct accagctgat catatacttt
     4801 tctatattct tgtaattctt tgatggtaat tagtggaatg ttgtgtttct tcgagaactg
     4861 aattaaatca tctgttctca tcattttgcc atcatgattc attatttcac aacataggcc
     4921 acactctttt agtccagcta attttaataa atcaacagtt gcttctgtgt gtccatttct
     4981 ttctagaaca ccaccttttt ttgcaattaa aggaaacatg tgtcctggcc tgcgaaaatc
     5041 agagggtgtt atatcttcag ctacacacat acgtgcggtc agtcctcttt cctcggcaga
     5101 aatacctgtg gtcgtttctt tataatcaat tgaaactgta aaagcagtct tatgattatc
     5161 tgtattgttt tcaaccatag gtgaaagcat taattgatta gctaaacttt cgctcatagg
     5221 catacaaatt aatcctttgg cataagtagc cataaaatta acattttctg ttgtagctgc
     5281 ttgtgcagaa caaattaagt ctccttcatt ttctctatcc ttgtcgtcta taacaagaac
     5341 aagtcgtccc ttctgcaatg cttctaatgc ttcttgtatt tttcgatatt ccattgactg
     5401 attatccttt ctgctaaaat ccattttgat ataatagttc cttagatatt tctgattttg
     5461 gagagttatc catcagtttt tgcacatatt tacctaagat atcattttca agatttactg
     5521 tactcccgac ttgtttactc ttaagaatgg tttgttccaa ggtatgaggg ataacagata
     5581 ctgaaaagtt tactttggag actttagcga cagtcagact aatgccgtca attgtaatag
     5641 atcctttttc aactattaaa tctaaaattt ctttttgtgt gttgatttga taccatacag
     5701 cattatcatc tttttttatt gacgagattt ttcctgtacc atcaatgtgt cctgtaacga
     5761 cgtgaccccc aagtcgaccg ttgacagata aggctctttc tagattcacc tcacttccat
     5821 gttttaatag agtaagagct gttcgactcc atgtttcatt cattacatca actgtaaagg
     5881 attgatgatt gaaatgagta actgtaagac agataccatt tactgctata ctatcgccta
     5941 aatggatatc cgttaatatt tttgaggctt taattgatag tttacaatta cgagagtctt
     6001 tctgtattct ttcaactttt ccgatttctt caattattcc tgtgaacatg gataaatcac
     6061 ttcactttct atgagatagt catttcctat ttgagaaaaa gcataaggtt tcaatctaat
     6121 agcgtcattt ggcaaagaaa tgccttcacc tccgacagga aacttggcac tgcctccaaa
     6181 aatttttggt gcaatatata ttttcagctc atcaacaatt tgttgttcca aagcactcca
     6241 attcattaga ctgccccctt ctagaactag gctatcaatc tgcatgtttc ctagatgttg
     6301 cattaaactc gataagtcta tatgattgcc ttttttcttt atggaaagta tttcacagcc
     6361 atgattttga tatagcttca ttttattttt gtcttcagag gaagtggcaa tgtaagtttt
     6421 aatatcattt gctgttttta cgattttaga ggtaagagga gttcgtaaat gtgtatcgca
     6481 tatgatacgg ataggatttt tcccttcctc caatctacat gtcagcaaag gatcgtcttg
     6541 aataacagta ttgactccca ccataattgc actaacatgg tgtcgtaact gatgcacatg
     6601 ctttcttgct tcttcttcag taatccattt ggattgattt gttttagtgg ctatttttcc
     6661 atccattgac attgcatatt tcataaaaac atagggtaca tgctgggtaa tatactttct
     6721 aaaacttttt attaagttaa gacactcatt ttctaaaatt ccaacagtaa cttgaagatt
     6781 attttcctca agtatcttta ctccttttcc agatacaata ggattacagt ctaggtttcc
     6841 aatgactact cttgtaatac cactatcgat tatagcatct atacagggag gtgttttccc
     6901 gaagtgacaa cagggttcaa gtgttacata aagcgtcgct ccgacagggg attctctaca
     6961 gtttttaaga gcatttctct cagcatgtgg gccaccaaaa aactcatgat aaccttgtcc
     7021 gatgatgtga ttatctttta caataactgc gccgaccata ggattgggat tgacgtaacc
     7081 agcccctttt tgtgccagtt ttattgctaa tttcatatat tttgaatcgc tcatctcact
     7141 acctccaaaa aaaatatacc ttgaataggg gactactcaa ggcatacaaa agaaaactta
     7201 tgcgattaac aaaaatactc tgaaatgaca agtaatcatt tcagagcacg caaaaagcac
     7261 aaatatactt ttatcttctt tcatccagac tatactgtcg gctttggaat ttcaccaaat
     7321 catgcctttc ggctcgtggg ctataccacc ggtagggaat tgcaccctgc cctgaagata
     7381 gttattcaat tacagatgat tatagtactt aattttgaat atgtcaacag ataaataccg
     7441 attgtttttg atatactgta tttgtgataa tcgattctcg ctcctcggat aaagaaaata
     7501 tgatatacta gataaacgaa ataagagaga aggaatacta tgtacgcata tttaaaagga
     7561 atcattacca aaattactgc caaatacatt gttcttgaaa ccaatggtat tggttatatc
     7621 ctgcatgtgg ccaatcctta tgcctattca ggtcaggtta atcaggaggc tcagatttat
     7681 gtgcatcagg ttgtgcgtga ggacgcccat ttgctttatg gatttcgctc agaggatgag
     7741 aaaaagctct ttcttagtct gatttcggtc tctgggattg gtcctgtatc agctcttgct
     7801 attatcgctg ctgatgacaa tgctggcttg gttcaagcca ttgaaaccaa gaacatcacc
     7861 tacttgacca agttccctaa aattggcaag aaaacagccc agcagatggt gctggacttg
     7921 gaaggcaagg tagtagttgc aggagatggc cttcctgcca aggtcgcagt gcaagcaagt
     7981 gctgaaaacc aagaattgga agaagctatg gaagccatgt tggctctggg ctacaaggca
     8041 acagagctca agaaaattaa gaaattcttt gaaggaacga cagatacagc tgagaactat
     8101 atcaagtcgg cccttaaaat gttggtcaaa taggagcaga gaatgacaaa acgttgttcg
     8161 tgggtcaaga tgaccaaccc gctctacatc acctatcatg atgaggagtg gggccagccc
     8221 ctccatgatg accaagcatt gtttgagttg ttgtgtatgg aaacctatca ggcaggcctg
     8281 tcttgggaaa cggtactcaa caaacgccaa gctttccgag aagcctttca tagctatcaa
     8341 attcactcag tcgcagagat gactgacact gaattggaag ccatgctgga gaatccagct
     8401 atcattcgaa atagagccaa gatttttgct acacgcgcta acgcccaagc ctttctacag
     8461 ttacaggcag agtacggctc ttttgatgcc tatctttggt cttttgttga ggggaaaact
     8521 gtcgttaacg atgttcctga ttatcgccaa gcgccagcta aaacaccctt atctgagaaa
     8581 ttagccaaag atctcaaaaa acgaggcttc aagttcacag gcccagtcgc cgtattgtct
     8641 tttctacagg ctgcagggct agttgatgac cacgagaatg attgtgagtg gaaaggtctt
     8701 aaatgatgtc taacaaaaat aagggaattc tgatttttgc gattctctat acagtcctct
     8761 ttgtgtttga tggcgttaaa ttgctggctt ctttaatgcc atctgtcatt gcaaattatc
     8821 ttgtttatgt agttttagct ttatatggct ccttcttatt caaggataga ttgatccaac
     8881 aatggaagga gattagaaag actaaaagaa aattcttctt tggcgtctta acaggatggc
     8941 tctttctcat tctgatgact gttgtctttg aatttgtatc agagatgttg aagcagtttg
     9001 tgagactaga tggacaaggt ctaaatcagt ctaatattca aagtaccttt caagaacaac
     9061 cactactgat agctgttttt gcttgtgtca ttggacctct ggtagaagaa ttatttttcc
     9121 gtcaggtctt attgcattac ttgcaggaac ggttgccagg tttactaagc attattctgg
     9181 taggacttgt ttttgctctg actcatatgc acagtttggc cctatcagag tggattggtg
     9241 cagttggtta cttaggtgga ggccttgcct tttctattat ttatgtgaaa gaaaaagaga
     9301 atatctacta tcccctactt gttcacatgt taagcaacag cctctcctta atcattttag
     9361 ctatcagtat agtaaaatga aataagaa
//
LOCUS       DAVBGY010000066         8974 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVBGY010000066 DAVBGY010000000
VERSION     DAVBGY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.066"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            168..854
                     /locus_tag="VQ620_001780"
     CDS             168..854
                     /locus_tag="VQ620_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2764363.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKAAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMADISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPL
                     EIAKKFIEVV"
     gene            855..1787
                     /locus_tag="VQ620_001781"
     CDS             855..1787
                     /locus_tag="VQ620_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2764364.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSPFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFFFPAPL
                     IFALILNEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGIINSLLSSLGMEP
                     ISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTLYEAADMDGANRWHKMWH
                     VTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVSDVLSTYVYRQGILNSDF
                     SYSAAVGLFNNIVALLFVLLANKIAKKLGEEGIV"
     gene            1798..2727
                     /locus_tag="VQ620_001782"
     CDS             1798..2727
                     /locus_tag="VQ620_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2764365.1"
                     /translation="MIFSKNTKKSNRDKETFVDYLFIFILIIITLLMLYPLWWVVMTS
                     LSEPVWLSKNVVKLFPLKIDITAYKFIFGDSKLWQAYGNSVLYAALSTIVSVAVCSMY
                     AYPLTIPEIRGKKFWNTLILIPMFFSGGMIPLYIQVANLGLLNTAWALVLPGAVSTYY
                     IILFRTQFKSIPMELRESARIDGASEFRIFWNLVLPLSKVIFMIIALYCIVGSWNSFT
                     PPLLYLNDDTKMPLSIYLRNLVVTNTTDTAELSLSAGNTYYQQIAAQGGGIGMRIAIK
                     MGTIVVSVIPIMMIYPFIQKHFVKGILLGSVKG"
     gene            2741..4180
                     /locus_tag="VQ620_001783"
     CDS             2741..4180
                     /locus_tag="VQ620_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764366.1"
                     /translation="MQNLKKYLLLDSGLVVGYTLENAQLKLNKLKKDTENNPLFGEEY
                     FAENPKLWEVRFDNSYPNVIYDKELKLYRCWYQTFVSDEASEETPLAERGEKEYIVKS
                     SRMTALCYAESKDGVKWEKPNLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRY
                     KLVSKLEFSPNLHYMVVVFSEDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVIT
                     RTWANNVRLSAITFSDDFINWSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHE
                     GDTLNENYDKVDLELKFSANLEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPI
                     IEDDKIWFYYMGGNGNHTGFRESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYD
                     DEIYILADIPENGYIKVAVGTKYGNAYSDFGQEDISLVQVSDGKYKLEFNKKSILDLG
                     NSPVAFKFEIENAKLYGIEGSIQQHYLKY"
     gene            4249..5826
                     /locus_tag="VQ620_001784"
     CDS             4249..5826
                     /locus_tag="VQ620_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2764367.1"
                     /translation="MVKLNKVLKGSVAFLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDETLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSQHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKSFDWQPLVYPEINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEELDVLTKSEAGLNTFFDENIIKFIEGQRDMSEWDSFINEAKSKDLDK
                     VTKVYNDAYKRVKDRMK"
     gene            5886..7346
                     /locus_tag="VQ620_001785"
     CDS             5886..7346
                     /locus_tag="VQ620_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2764368.1"
                     /translation="MYDVVIIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIKKGGTVFNSMEELMNVNDFYQITIPYQPE
                     VYESVLTDMLIESKVDILLNVEVNNIIYDSKNIKSLEILSNQEVKLIDSKVFIDATGN
                     ATIASASGADVYEKNSSYGCLMRVGGVDISKTIEHIEKTKQWNYIEGYNQWLEEVLAN
                     RKYRATGAVLKDPVSYDHAPMLSKDDHFMNDEKWNYIKERWEKFRFIYTLEVSLFRNE
                     LKKAVESGDFNFEIYHDDKSGVTMNGDGISYGSWGQNVALINVAKAFGFNPKYMEDES
                     HANILSRKYNLTFLSFLKKYVPGFENSFILDQGTRATNRSCRHIDNSSMMDEILDFPM
                     YTFKTMYSYQTPKEITYKSIVGGKLNNLFVIGKGAYQSESFRSQISCMLMGISSAAAA
                     KTIIEDKSNTLNINRDLFKSNLDILFTWEFSNWITD"
     gene            7368..7823
                     /locus_tag="VQ620_001786"
     CDS             7368..7823
                     /locus_tag="VQ620_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET2764369.1"
                     /translation="MNDTNKNQYFVYEFDTSFVEMSYSQFFEGYCILFSKEPYPSLSD
                     MPMEFRKEFLMEMSILGDAMLEVLNAYRINYDILGNSTPVLHAHLFPRYMWEEEERRK
                     TVVHRYDSSVFSNPEFEFTIEKHGPIIKKLRSAIDRHYREYQQLLDDSN"
     gene            7910..8315
                     /gene="cdd"
                     /locus_tag="VQ620_001787"
                     /pseudo
     CDS             7910..8315
                     /gene="cdd"
                     /locus_tag="VQ620_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221146.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
     gene            8328..8777
                     /locus_tag="VQ620_001788"
     CDS             8328..8777
                     /locus_tag="VQ620_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2764370.1"
                     /translation="MELVEFKNGHVNTVHEHIAITKVKEFLNNHDILKDKGGIHKIST
                     DFFYNIIDMETTTEENRPWESHKEYYDVHYLLNGEETILYNFLSQMELSEYKVDDDWQ
                     QMNGTALFSIKLKKDMLLLLEPNDAHRTGLLVEEPLNIKKVVFKVKI"
BASE COUNT         3114 a         1179 c         1675 g         3006 t
ORIGIN      
        1 gtgtattgaa tctataacag tacgccttaa atgctaaaat atttctataa attaatttaa
       61 ctttcctgat agggctgttc atatcttatt tcaattcact atataaataa aattgtagta
      121 gatgttattg aattagtaaa aaaaataaaa aaacggaggt tttctgaatg gaaactaaaa
      181 aaattaaaaa tttaaaaggt caaataattg tttcctgcca agcgttagaa ggagagccac
      241 tttatactcc aaatggagga gtgatgccgt tattagcaaa agccgccttt caagcaggcg
      301 ctaaaggtat tcgtgctaat tctgtccgcg atatttcaga aataaaagaa gaagttgact
      361 taccaattat aggtatcata aaaagagatt acgatggctt tgaacctttt atttcagcaa
      421 ctatgaaaga gattgatgaa ttagttagtg aaggagtaga cattttagct ttggattgta
      481 ctaatcgttc aagaccaggt tatgataata ttactgactt tattcatgat attaaagtca
      541 agtatccaaa ccaattatta atggctgata tttctacttt tgaagaaggg aaagtagctg
      601 ctgaatcggg agtagatttt gtgggtacga cattatctgg ttatacacca tatagcccta
      661 aaaaagataa tcctgatttt gagttagttg aaagattggt aaaagagctt gacgtaccag
      721 ttattgctga aggaagaata agtactccgg aacaagctag aaaaatgtta gatttaggtg
      781 cttacgcagt ggttgttgga ggagctatca ctcgtccttt ggaaatagcg aagaagttta
      841 ttgaggtagt ttagatgaat caagaaatat taaaaaagag taagattcaa ttgacagttg
      901 aacatattca aaaatattgg attttatatt taatgatgat tccagcaact gttctactaa
      961 ttttatttac gtatggacca atgtacggca ttattatggc ctttcaagac tttactgtct
     1021 tcaagggata tactggaagt ccatttgtcg gtttaaaaca ttttcaaaga ttgttctcag
     1081 atcctttgtt ttatagattg ttcaagaata cgttcatggt aggggtttta gattttttat
     1141 tctttttccc agctccgctt atttttgcac tcatattaaa cgaagtcaga aaagtaagat
     1201 ttaagagtgt agttcaatct attagttatc ttcctcattt tattccccta gtggttatgg
     1261 ttggtattat ttttgaacta tttggttcat atggtattat taacagttta ctttcatcac
     1321 ttggaatgga gccaataagt ttctttacaa aatctgaatg gttcttgcct ctctacattg
     1381 gttcaggagt atggaaaaca ataggttggg gttctatcat ttatatggga gcattaacca
     1441 atatagattc aactttatat gaagcagcag atatggacgg ggctaataga tggcacaaaa
     1501 tgtggcatgt aactctacca agtttaagac ctacagtagt tactttgttt atactaaatg
     1561 ctggtggtat catgcaagta ggatttgaaa aggtattctt gatgtctagt cctgcaacat
     1621 atgaagtatc tgatgtatta tctacttatg tttatagaca aggtatttta aattctgatt
     1681 tcagttactc agctgcagta ggattattta ataacatagt agctttgtta tttgttttat
     1741 tagctaataa gattgctaaa aaacttggcg aagaaggaat tgtataaggg gggtaaaatg
     1801 attttttcaa aaaatacaaa aaagagtaat agagacaaag aaaccttcgt tgattatctc
     1861 tttatattca ttttaataat aataacctta cttatgttgt atccattatg gtgggtagtg
     1921 atgacgtcac tttcagaacc agtttggcta tctaaaaacg ttgttaaatt atttccctta
     1981 aaaatagata ttactgcata taagtttatt tttggggatt ctaaactatg gcaagcatat
     2041 ggtaatagtg tattgtatgc tgcactatct acaattgtta gtgttgcggt atgtagtatg
     2101 tatgcctatc cacttacaat tcctgaaatt aggggtaaaa aattctggaa tactcttatt
     2161 ttgattccaa tgttctttag tggaggaatg atcccgcttt atatacaagt tgcaaacctc
     2221 ggtttattga atactgcttg ggctcttgtt ttacctggtg ctgtttctac atattatata
     2281 attttgttta gaacacagtt taaatcaatc ccaatggagc ttcgtgagtc tgctagaatt
     2341 gatggggcaa gtgaatttag aattttttgg aatttagtat taccattatc aaaagttatt
     2401 ttcatgatta tagcacttta ttgcatagtt ggttcatgga atagttttac tcctccatta
     2461 ctttatttga atgacgacac taagatgcct ttatctattt atttgagaaa tttagtcgtt
     2521 acaaatacaa ctgatactgc tgaattatca ctttctgcag gtaatacata ttaccaacaa
     2581 attgctgctc aaggtggagg tattggtatg cgtattgcta ttaagatggg tactattgtt
     2641 gttagtgtta ttccaattat gatgatttat ccatttattc agaaacattt tgttaaggga
     2701 atcctgctag gttctgtgaa aggttaataa ggtaattatt ttgcaaaatt taaaaaaata
     2761 tcttttatta gatagcggtt tagtagttgg gtatacatta gaaaatgctc aattaaaatt
     2821 aaataagcta aaaaaagata ctgaaaacaa tccactattt ggtgaagagt attttgccga
     2881 aaatcctaaa ttatgggaag ttcgttttga taattcatat ccaaacgtta tctatgataa
     2941 agaattgaaa ttatatagat gttggtatca aacatttgtt agtgatgaag caagcgaaga
     3001 gactccttta gcagaacgtg gagagaagga atacattgtc aagtcatcta gaatgacagc
     3061 actttgctat gctgaaagta aagatggagt aaaatgggaa aaaccaaatc tcaatctagt
     3121 aaaatttaaa ggttcaaaag ataataatat tgttctgatg catgcacacg gtgcaggtat
     3181 catgctagat gaacaggagt tggatcctaa gaaacgatat aaattagttt caaaattaga
     3241 attttctccg aatcttcatt acatggttgt tgttttttca gaagacggta ttcattttga
     3301 cgaacctatt ccatggaaaa aatggaatcc tgcagcagat agtcataatt ttccatttag
     3361 agatccaaaa actggtaaat acgctgttat tacaagaacc tgggcaaata acgtacgtct
     3421 aagtgcaatt actttcagtg atgattttat taattggtca gaacctaaag aaatccttag
     3481 gggagatgga tttgaagatc aaatttattc tatgccagta atttcatacg cggatatcta
     3541 tttaggtttt gcttcaatgt accatgaagg cgacacttta aatgaaaact atgataaagt
     3601 agacttagaa ttgaaattct ctgctaattt ggaacaatgg gatagtgtat gtaagggcga
     3661 ctatctcatt gaaaggggag gtggagtata cccgcagaat gctgattttg atgctagttg
     3721 tattttcacg gctgctccaa taattgaaga tgataagatt tggttctact atatgggtgg
     3781 aaatggtaat cacactggct tccgagaaag ttcattagct agaggatata ttaagaaaga
     3841 ccaatttgct tattatacta ataagagaga aggaattcca gctaaaatat ctacagttag
     3901 cttccaaact tacgatgatg aaatttatat cttagctgat attcctgaaa atggttatat
     3961 taaagttgcg gtaggtacta aatacggtaa tgcttattca gatttcggtc aggaagatat
     4021 ctctcttgtt caagtttccg atggtaagta taagttggaa tttaataaaa aatctatctt
     4081 agatttaggt aattctccag ttgcgttcaa atttgaaatc gagaatgcaa aattgtatgg
     4141 tattgaaggt agtattcagc aacactatct caaatattaa ccagaataat tcgttaacaa
     4201 ataatctcta tattagtgga gatagatata taaaatggag gtcttattat ggtaaagctt
     4261 aataaggtat taaaagggag tgttgcattc ctgagtgcaa gtttattgct agcagcttgt
     4321 ggcaataacg ctaattcaaa gcccgctgaa aaaacagctg atggtaaaac gaaaatttcg
     4381 tttctatcta catggcaaat taatgatgtt cgtccgccta aagatgaaac acttgttggt
     4441 caatggttag aagaacagct aggtattgat attgatcttg taactattcc tggcgaggga
     4501 gtaagttcta aagtaaattc tcttattact agtaaccagt taccgactgt tttgttgaca
     4561 acaggagata agagtgatat cgctggtatc aacaaacttg gtaaacaggg tgcattctta
     4621 gatttgtcac aacacatgga caaactacca aactataaga agtaccttga aaaaaataat
     4681 gcattagctc aaatagaaga tgatgaaaaa catatttatg ctttcacaaa attctttact
     4741 gatgagaata tcatgtatac tacaccaatt ctgagaaaag acttacttgt tggttcagaa
     4801 tttgaagatc tttcaaaaat taaaactgtt gatgactata caaaagtttt gaaatatttg
     4861 actgaaaaac aaggttctcc agcatttatt caacgtaatg gctatgaagg cttcatgaag
     4921 cgagttacac cattgtggaa tttaagccat cgtacatact atgactatga atcagattca
     4981 tataaacatc cagttgaaca acctcaattg aaacaattcg ttgaatggtt gaaagaatta
     5041 cgtaaagata acgtacttca ccctgactgg gcagttatga aagatgaaac ttgggaaggt
     5101 ctattggctt ctaacaaggg ttcattcact gttgaccgta tgaatatcat tggagataat
     5161 aactttgata aatcatttga ttggcagcca cttgtttatc cagaaattaa tggaaaacgt
     5221 tatttgcaac cgaaaacacc gtacatttct gattcaggtt gggtaatcaa tgccaatgca
     5281 ccaaaagaag aaattgataa agcgttagaa ttctttaatt tcttatacgc tgatgaaaat
     5341 caagaaaaac ttgctattgg atttgaaggt aaaacattta cacgtgaaaa ccctaatact
     5401 caagcaggta ttaaatggtt gattcaaatt tacggtgaaa atgctggaaa tgataaagca
     5461 gaactgctgt ttaaacatgg taaccaagca tttactcgcg ttcaaactaa attagatatt
     5521 gaggcacagc caggaacatt ccctaaagtt atgtatgatc aagtagataa aattaagaaa
     5581 gatttaggtg gttttagacc agatactccg gttctttcct ttacaactga agaattagat
     5641 gttttaacta aatctgaagc aggattaaat actttctttg atgaaaatat tattaaattc
     5701 attgaaggac aaagagatat gagtgagtgg gattcattta tcaatgaagc taaatctaaa
     5761 gacttggata aagttacaaa agtatataat gatgcctaca aacgtgtcaa agatagaatg
     5821 aaatagacta aatggtagct ctctggtttt tcagagagct actactgcct gagggggagt
     5881 aagctttgta tgatgtagta attataggtg caggaagttc gggcgcattg gctgcaattg
     5941 cttcggggcg tctagggtta aaaacttgtc taatagagaa gggaagtaga attggtggag
     6001 ttcctattaa tacactaatg gggtcatttg ctaatctata ttttgatgat tctggtagag
     6061 taaatgcaag taaaattgtt ggggaattaa tagaacgtat tatcaaaaaa ggtggtactg
     6121 tctttaactc tatggaagaa ttgatgaatg taaatgattt ttatcagatt acaattccct
     6181 atcaaccaga agtttatgaa tcggttttaa ctgatatgtt gattgagtct aaagttgata
     6241 ttttactaaa tgttgaagtt aataatatta tttatgatag taaaaatatt aaaagtcttg
     6301 aaattttaag taatcaggaa gttaaattga ttgattctaa agtttttatt gatgctacag
     6361 ggaatgcaac aattgcttct gctagtggag ctgatgttta tgaaaaaaat tcttcttacg
     6421 gttgtttaat gagagttgga ggagttgata tctcaaagac tatagaacat attgaaaaaa
     6481 caaagcagtg gaactatata gaaggatata atcagtggtt agaagaagta ttagcaaatc
     6541 gaaagtacag agccactgga gcagttttaa aagatccagt ttcttatgat catgccccta
     6601 tgctttcaaa agacgaccat tttatgaatg atgaaaaatg gaattacatt aaagaaagat
     6661 gggaaaaatt tagatttatt tacactttag aagtatcact atttaggaat gaattaaaaa
     6721 aagctgtaga atctggggat tttaattttg aaatttatca tgatgataaa tctggagtca
     6781 ctatgaatgg cgacggtata agctatggtt catggggtca aaatgtagca ttaattaatg
     6841 ttgcaaaagc atttggattc aatcccaaat atatggaaga tgaatcacat gctaatattc
     6901 tttctagaaa atataattta acatttttat cttttcttaa aaaatatgta ccaggttttg
     6961 aaaattcttt tatccttgat cagggaacta gagctactaa cagatcatgt aggcacattg
     7021 ataattcatc tatgatggac gaaattcttg attttccaat gtatacattt aagactatgt
     7081 atagctatca aactcctaaa gaaataacgt acaaatcaat tgtaggtgga aaattgaata
     7141 atttgtttgt aataggtaaa ggtgcttacc aatccgaaag ttttagatca caaattagtt
     7201 gtatgttaat ggggatatca tcagcagcag cggcaaaaac tattattgaa gataaaagca
     7261 atactctaaa cattaataga gatttgttta aatcgaactt agatatatta tttacatggg
     7321 agttttctaa ttggattact gattaacaaa taataaggga atattaaatg aatgatacta
     7381 ataaaaatca atactttgtt tatgaatttg atactagctt tgtagaaatg tcttattctc
     7441 agttttttga aggatattgt attctttttt caaaagaacc ctatccttct ttatctgata
     7501 tgccaatgga atttagaaaa gaatttttaa tggaaatgag tatattaggt gatgcgatgc
     7561 ttgaagtgtt aaatgcctat agaattaatt atgatatctt aggaaattca acacctgtac
     7621 ttcatgctca tttatttccg agatacatgt gggaagagga agagagaaga aaaactgtag
     7681 ttcatagata tgattcttca gtttttagca atcctgagtt tgaatttact atagaaaagc
     7741 atggaccaat tattaaaaaa ctgaggtctg ctattgatag acattataga gagtatcaac
     7801 aattattaga tgattcaaac tgaactccac attttcgatt agatttaata ccttgtatat
     7861 tacaactagg ttgtggatga tataattttt tgaaaaatat attttaggaa tgacttataa
     7921 agcaattata tcagaggcta ttaaatcaat ggccaaagct tatgctcctt attctgattt
     7981 taaggttgga gctgcgttag taactgaaga taaaaaattt tttctggatg taatatagaa
     8041 aatgctgctt tttctcctac ttgctgtgca gaacgagtat ctttttataa agctgtattt
     8101 aaaggagaac gttcgttttc aagaacatgt attgtaggtg gaaaaaatgg tatcttatca
     8161 tcttttatag caccttgcgg agtgtgtcgc caggtaatga tagaatttgt aatcctgatt
     8221 catttgaagt tatattggct agtgatgttg atacttataa agtatataaa cttaaagaac
     8281 tactaccagt tagttttaat caagaaaatt tataacagga gggaactatg gaattagttg
     8341 aatttaaaaa tggacatgtt aatacggttc atgagcatat cgcaataacg aaagtaaaag
     8401 agtttctaaa taatcatgat atattaaaag ataaaggtgg catacataaa atttctactg
     8461 atttctttta caatataatt gatatggaaa ctacaactga agagaataga ccgtgggaaa
     8521 gtcataagga atattatgat gtccattatc tgttaaatgg agaagaaaca attttatata
     8581 acttcttgtc tcaaatggaa ttgtctgaat ataaagtaga tgatgattgg caacaaatga
     8641 atgggacagc tttatttagt attaaattaa agaaagatat gttacttttg ttggaaccaa
     8701 acgatgcaca tagaacaggc ctattggttg aagaaccact taatattaag aaagttgttt
     8761 ttaaggtaaa aatttaactg aaagtacttt cggggattaa gaattttaaa atatttaaga
     8821 aattgtattc taaagttcta aaattataga tatttaattt aaagcttgtt aaaatagaat
     8881 agtaacactc atctgctttc aaaaagcatt ctattccatc tccgattaac gatggacttt
     8941 atcacctcct tctccagtcc ttgtatgaca tctt
//
LOCUS       DAVBGY010000067         8843 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVBGY010000067 DAVBGY010000000
VERSION     DAVBGY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8843
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.067"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            149..1168
                     /gene="galE"
                     /locus_tag="VQ620_001789"
     CDS             149..1168
                     /gene="galE"
                     /locus_tag="VQ620_001789"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2764371.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            1170..2135
                     /locus_tag="VQ620_001790"
     CDS             1170..2135
                     /locus_tag="VQ620_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2764372.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTHYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(2153..2392)
                     /locus_tag="VQ620_001791"
     CDS             complement(2153..2392)
                     /locus_tag="VQ620_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET2764373.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEALINGDNPLPLV"
     gene            2444..2920
                     /locus_tag="VQ620_001792"
     CDS             2444..2920
                     /locus_tag="VQ620_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET2764374.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            2929..3600
                     /locus_tag="VQ620_001793"
     CDS             2929..3600
                     /locus_tag="VQ620_001793"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET2764375.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            3730..4068
                     /locus_tag="VQ620_001794"
     CDS             3730..4068
                     /locus_tag="VQ620_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HET2764376.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            4071..4700
                     /locus_tag="VQ620_001795"
     CDS             4071..4700
                     /locus_tag="VQ620_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HET2764377.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            4940..6091
                     /locus_tag="VQ620_001796"
     CDS             4940..6091
                     /locus_tag="VQ620_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2764378.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            6409..7158
                     /gene="truA"
                     /locus_tag="VQ620_001797"
     CDS             6409..7158
                     /gene="truA"
                     /locus_tag="VQ620_001797"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET2764379.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQAI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFILDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            7148..7921
                     /locus_tag="VQ620_001798"
     CDS             7148..7921
                     /locus_tag="VQ620_001798"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET2764380.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            7899..8360
                     /locus_tag="VQ620_001799"
     CDS             7899..8360
                     /locus_tag="VQ620_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2764381.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            8590..8799
                     /locus_tag="VQ620_001800"
                     /pseudo
     CDS             8590..8799
                     /locus_tag="VQ620_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2525 a         1641 c         1976 g         2701 t
ORIGIN      
        1 caaggcgacg ttgacgtggt ttgaattgga ttttcgaaga gtataagcat tttactgaaa
       61 ttttatcagg tagtttagga tggcaaatga cattccctgt caaaaatggt aaaataagaa
      121 aaaataatct gagaatcgag gaaaaaagat gcaagaaaag attttggtaa ctggtggtgc
      181 cggttttatt ggaacccaca ctgttattga attgatccaa gcaggccatc aagttgttgt
      241 ggtggataat cttgtcaata gcaatcgtaa gagtttagaa gttgttgaaa gaatcacagg
      301 agttgagatt cctttctatg aggcagatat tcgtgacaca gataccctca gagatatttt
      361 caagcaggaa gaaccgactg gtgtcattca ctttgctggt ttgaaggctg ttggcgaatc
      421 aacacgtatc cctcttgcct actatgacaa caatatcgct ggaactgtca gccttttgaa
      481 agccatggaa gaaaacaact gtaaaaacat catcttcagt tcttctgcga cagtttacgg
      541 ggatccgcac acagtgccca tcttggaaga tttcccactt tcagtgacca acccatacgg
      601 tcgtaccaag ctcatgctag aggaaatttt gactgatatt tacaaagcag actcagaatg
      661 gaatgttgtc ttgcttcgtt actttaaccc aatcggagcc catgagagtg gtgatttggg
      721 agaaaatcca aacggtattc caaacaatct cttgccatat gtgactcaag tagccgttgg
      781 aaaattagag caagtgcaag tgtttggaga cgattacgat acggaagatg gaacaggtgt
      841 tcgtgactat atccacgttg tcgatttggc taagggtcac gttgcagctt tgaaaaaaat
      901 ccaaaaaggt tcaggactaa acgtttataa ccttggaact ggtaaaggtt actcagttct
      961 tgaaattatc caaaacatgg aaaaagcggt gggacgtcct attccttacc gcatcgtaga
     1021 acgtcgccca ggtgatatcg ctgcctgcta ctcagaccca gcaaaagcta aagcagaact
     1081 cggttgggaa gcagaactcg acatcaccca aatgtgtgaa gacgcatggc gttggcaaag
     1141 caagcatcca aatggatttg aagactaaga tgatgatttc aatcatcgtc ccttgtttaa
     1201 acgaagagga agtacttcct cttttttatc aggctctgga agctttactt ccagatttgg
     1261 aaacagaaat cgagtatgtc tttgtcgatg atggatcaag tgatgggacc ttggaactct
     1321 taaaggccta tcgggagcaa aatccggcag ttcattatat ttctttctct cgaaattttg
     1381 gcaaagaagc agccctttat gcaggcttgc aatatgcgac aggagatttg gtggtggtga
     1441 tggatgcaga cctccaagat cctcctagta tgttgtttga gatgaaaaat gtactagaca
     1501 aaaatgtaga cttggactgc gttgggacac ggagaactag tcgggaggga gaacccttct
     1561 ttcgcagttt ctgtgctgtt ctcttttatc gcctcatgca aaaaatcagc ccagtagctc
     1621 tgccgtcggg tgtccgtgac tttcgtatga tgagaaggtc tgtggtcgat gccattttaa
     1681 gcttgactga gtccaatcgt ttttctaagg gactctttgc ctgggtcggc tttaaaaccc
     1741 actatctgga ctatccaaat gtcgaaaggc aggctggcaa gaccagttgg agttttaggc
     1801 aactcttttt ttactccatt gaagggatta ttaatttttc agattttccc ttgattatag
     1861 cctttgtagc tggtctccta tcttgttttc tttctctgct gatgaccttt tttgttgtgg
     1921 ttcgaaccct cattttgggc aatccgacat cgggttggac ctctctgatg gctgtcattc
     1981 tctttcttgg aggcattcaa ctcttgacca ttgggattct cggcaagtat atcagtaaga
     2041 tttatttaga gactaaaaaa agaccacttt atcttatcaa agaaaaaagt gaccttcctg
     2101 attttacaga aaaaaataaa gtgaaaagac tataatttta catggaaatg tgctaaacta
     2161 gagggagtgg attatcgcca ttgattaagg cttcctggtt tccaatcagg gcgcgagttg
     2221 ggcaattttt aacagcctct aagacatcct gactaggaga aatttctttt tccagttggt
     2281 cagggtcatc gtaaaaacgc acgattccat tatcgtggta atcaaataaa tcagaataag
     2341 tttggcaaag cccacaggcg atacatcgtt caggtataag tgtgattttc atgttcatat
     2401 ttatattgta ataagaaaag taaaaaaaaa caaggagtaa ggtatggcaa aagaaccgtg
     2461 gcaagaagat atctatgatc aagaagaatc aagagcagag cgtcggcatc gaaaccacgg
     2521 aggggctgat aggatggcta atcgtatttt gacgatccta gctagtattt tctttgtaat
     2581 tgtggtggtg atggtcatcg ttctcatcta tctatcatcg ggggggagta atcgcacagc
     2641 agccttaaaa gactttcatg attctgatgc aagtgtagta caaatctcat cttcaagcag
     2701 ttctcagcct gagcagagtt cagagccaga atctacttct agtagttcag aagaagctgc
     2761 taatcctgaa ggaacgatta aagttctcgc aggagaaggg gaagcagcta ttgccgctcg
     2821 tgcaggaatc tccattgctc agttagaggc cttgaatcct gggcacatgg ctacaggatc
     2881 ttggtttgct aatccaggtg atgttataaa aataaaatag gagccgatat gaaaacaatt
     2941 caaattgcta ttgatggtcc tgcttccagc ggtaagagta cggtcgcaaa gattattgct
     3001 aaggattttg gattcaccta ccttgataca ggagctatgt atcgtgcagc gacctatatg
     3061 gctcttaaga accaattagg agttgaagaa gtcgaagccc ttctagcctt gttggaccag
     3121 catccaatca gctttggacg ttcagaaact ggagaccagc ttgtttttgt aggagatgtg
     3181 gatattaccc atcctatccg tgaaaatgaa gtgaccaatc atgtttctgc tattgcagca
     3241 attcctgaag tgcgtgagaa actggtttct ctccaacaag aaattgccca gcaaggcggg
     3301 attgtcatgg atggtcgcga tattggaact gttgtattgc cacaagcaga attgaaaatt
     3361 ttcctagtag cttctgttga tgagagagca gagcgtcgtt acaaggaaaa tattgccaag
     3421 ggaattgaaa cagaccttga aaccctaaaa aaggaaattg ctgcgcgtga ctacaaggat
     3481 agtcatcgtg agacttctcc tctcaaacaa gcagaggatg ctgtctacct tgatacaact
     3541 ggtttgaaca ttcaagaagt agttgaaaaa atcaaagcag aagctgaaaa aagaatgtaa
     3601 atgtagaaaa gccgatagat tgatatcggt tttttctaaa tttgtcaaaa tgctgatttt
     3661 agggtataat agttgctgtt cgagaaattt tgattttcaa agaatagtga atgatgataa
     3721 ggagaaatca tgaacaattt accaaattgt ccaaaatgta actcagagta tgtctacgaa
     3781 gacggtgccc tactggtttg cccagagtgt gctcatgagt ggaatcctgc tgaagttgca
     3841 gaagtagaag aaggtcttgt cgctatcgat gccaacggaa ataaattggc tgatggtgat
     3901 acagtaactc tcatcaagga cttgaaagta aaaggtgcgc caaaagattt gaaacaaggg
     3961 acgcgcgtga aaaatatccg catcgtagaa ggcgaccaca atatcgactg taagattgat
     4021 ggttttggtg ccatgaaact taaatcagag tttgtgagga agatttaagc atgtcaaaac
     4081 actataaact tgtattttat agccgtatct tcttgtttct agcggctttt acgggagttt
     4141 atcttgaaat tactaagcat ggtggttttg ggatgcttct ctattacacg gttctgtcca
     4201 acctcttggt aactattttt accctttatc ttctaaaggt tatgagccgt gtaggtgaaa
     4261 attggcaaag accaagtctc ttgcgcttaa aaggtggggt caccatgagt atcatgatta
     4321 cctgtgtgat ttaccatttc ctcttggcgc ccattgcgac taatttctat accctagaaa
     4381 atttcctttg ccactatatc gttcccatct ggtttttagc ggataccctc ttttttgaca
     4441 aacagggtca atacaagatt tgggatccag cagtgtggac cattttaccc tttctgtata
     4501 tgatgtttgc tctttttaat ggcttggttc taaaactcaa tattccaaat gccaaggata
     4561 atcctttccc ttacttcttt ttgaatgtga acaagggttg gaatgttgtg tttaagtggt
     4621 gtctgattat ctttgttgcc tatatggtag caggatttat tttctacttt atcaagcaaa
     4681 tcaagagaaa gtcatcctaa tactcttcga aaatctcttc aaaccacgtc agtttccatc
     4741 tgcaacctca aaacactgtt ttgaacaacc tgcggctagc ttcctagttt gctctttgat
     4801 tttcattgag tataagatac aagggattca ttttcgaatt taagaaggag tctacaaacc
     4861 aatgaggctt cttcttttct tgcttatttg ataactatca aaaaagtaga agattaagag
     4921 aaatatagaa agagatttca tgaaacaatt tttagaacgg gccagcattt tggctctctc
     4981 cctcgttttg attacctcct tttccatttc gagtgcccta ccagccatgt ttgactatta
     5041 tcagggttat tctaaggaac aaattgagct cttggtgagc ttgccttcct ttggaatcat
     5101 gatgatgtta ctgctaaatg gtttcttaga aaaaatattt cctgagcgct tacagattag
     5161 tttgggcttg ctgattttat cattgagcgg tacagctccc ttctggtacc aagcctatcc
     5221 ctttgtcttt ggaacacggc ttctctttgg tttgggtctt gggatgatca atgccaaggc
     5281 catttctatt atcagtgaac gctaccaagg aaaaaggcga attcagatgt tagggctacg
     5341 cgcttctgca gaggtcgttg gagcttctct cattaccttg gccgtcggtc agttgttggc
     5401 ctttggttgg acagctatct ttctagccta tagtgctgga tttttggtgc tgccccttta
     5461 tctgctcttt gtcccttatg gaaaatcaaa gaaagaagtc aagaaaagag cgaaggaagc
     5521 aagtcgttta actcgagaaa tgaaaggctt gatttttacc ttagctatcg aagcggcagt
     5581 tgtagtttgt accaatacag ctattaccat ccgtattcca agtttgatgg tggaaagagg
     5641 attgggggat gcccagttat ctagttttgt tcttagtatc atgcagttga tcgggattgt
     5701 ggctggggtg agtttttctt tcttgatttc tatctttaaa gagaaactgc tcctctggtc
     5761 tggtattacc tttggcttgg ggcaaatcgt gattgccttg tcttcatcct tgtgggtggt
     5821 agtagcagga agtgttctgg ctggatttgc ctatagtgta gtcttgacga cggtctttca
     5881 acttgtctct gaacgaattc cagctaaact cctcaatcaa gcaacttcat ttgctgtatt
     5941 aggctgtagt ttcggagcct ttacgacccc attcgttcta ggtgcaattg gcttactaac
     6001 tcacaatggg atgttggtct ttagtatctt aggaggttgg ttgattgtaa tctctatctt
     6061 tgtcatgtac ctacttcaga agagagctta ggattgattc ctaagttttt cttttgatac
     6121 tcaatgaaaa tcaaagagca aactatagtt gattgagttt ggaatagtac gctgtagctg
     6181 ctaaaatatt tctagaaatt aatttgactt tcctaataga gttgttcata tcttatttca
     6241 atctactata gaaagctagc cgcaggttgc tcaaaatagt gttttgaggt tgcagatgga
     6301 aactgacgtg gtttgaagag atttttgaag agtatgagat ttttataccc atacaatatt
     6361 tgttttaggt ccttgtttac agactaattt cttgataaaa tagaggctat gacgagatat
     6421 aaagcaacta tttcctatga tggttatgcc tttgctggct ttcagcgcca gcctcatgcg
     6481 cgtagcgttc aggaagaaat tgaaaaaacc ttgaccagat taaataaagg gcaagccatt
     6541 actgttcacg gtgctggtag gacagatagt ggggttcatg ccctgggaca ggtcattcat
     6601 tttgacctgc cttatcagat ggatgaggag aaactccgtt ttgccttgga tacccagtct
     6661 cctgaagata ttgatgtgat ttcgattgag cttgtggcag atgattttca ttgccgttat
     6721 gccaaacata gcaagaccta tgagtttatt ttggatagag gacgtcccaa aaatcctatg
     6781 cgccgtcact atgccaccca ctttccctac ccactcgatg tggaacgaat gcagattgca
     6841 atcaaaaagc tagagggaac ccatgatttt accggtttta cagcctctgg gactagtgta
     6901 gaggataagg ttcgcaccat cacagaagct agtttaatag tcgatgagac aggacaattt
     6961 ttgaccttta ccttttcagg aaatggtttc ttgtataaac agattcgcaa tatggtgggg
     7021 actcttctca aaatcggtaa caaccgcatg ccagtagagc agattgatct catcttggag
     7081 aagaaggaca ggcaacttgc aggtcccact gcagcaccaa atggtttgta tttaaaggag
     7141 attcgttatg aagaataatc gtattttagc actttctgga aatgatattt ttagtggtgg
     7201 tggactgtca gctgatttgg ctacctatac cttgaacggc ttgcatgggt ttgtagcagt
     7261 gacttgtttg acagccttga cagaaaaagg atttgaagtc tttccaactg atgataccat
     7321 ttttcaacat gaattagata gcttgcgtga tgtggaattt gggggaatta agattggtct
     7381 tctccctact gtcagtgtgg ctgagaaggc cttggacttt atcaaacaac gcccaggagt
     7441 acctgtggtg ttggatcctg tcttggtctg caaggaaacg catgatgtag ctgtcagtga
     7501 gctctgccaa gagttgattc gctttttccc ttatgtcagt gtgattacgc ctaatctccc
     7561 agaagcagaa ttattatccg gtcaggaaat taaaaccttg gaagacatga aaactgcagc
     7621 gcagaaattg catgatttag gagcgccagc agtcattatc aagggaggca atcgtcttag
     7681 tcaggacaag gctgtggatg tcttttatga tggacagacc tttactatcc tagaaaatcc
     7741 agttatccaa ggccaaaatg ctggtgcagg ttgtaccttt gcctctagca ttgccagtca
     7801 cttgattaaa ggtgataaac ttttgccagc agtagaaagc tctaaggctt tcgtttatcg
     7861 tgctattgca caagcagatc agtatggagt aagacaatat gaagcaaaca aaaacaacta
     7921 aaatcgccct tgtatcccta ttaaccgccc tttctgtggt tctaggttat ttcttaaaaa
     7981 tcccaacacc tacaggaatt ctaactcttt tagatgctgg tgtcttcttt gcggcctttt
     8041 actttggtag tcgtgaagga gcggtagtcg gaggactagc aagtttcttg attgacctct
     8101 tatcaggcta ccctcagtgg atgttcttta gcttggtcaa ccatggcttg cagggatttt
     8161 tcgcaggatt taaaggaaaa agtcagtggt taggccttat tttagcaact attgccatgg
     8221 taggaggcta cgccttgggt tctactttga tgaatggctg ggcagcagcc ctcccagaaa
     8281 ttctaccaaa tttcatgcaa aatatggtag ggatgattgt aggatttatt cttagtcaaa
     8341 gtatcaagaa gattaagtaa tactcttcga aaatctcttc aaaccacgtc agcttccatc
     8401 tgcaacctca aaacactgtt ttgagcagcc tgaggctagc tttctagttt gcactttgat
     8461 tttcattgag tataaagagg ttgagtaaaa agtatttcaa ataagaaaaa cgcatagtat
     8521 caggtgttga atgaactgcc ccccaaaaag ttagacagaa aaaatctaac tttttggggt
     8581 gtttttatta tgaaattaag ttatgatgat aaagttcaga tctatgaact tagaaaacaa
     8641 ggatatagct tagagaagct ttcaaataaa tttgggataa acaattctaa tcttaggtac
     8701 atgattaaat tgattgatcg ttacggaata gagttcgtca aaaaagggaa aaatcgttac
     8761 tattctcctg atttaaaaca agaaatgatt cataaagtct gacatgaagg ctggactaaa
     8821 gatagagttt ctcttgaata cgg
//
LOCUS       DAVBGY010000068         8781 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVBGY010000068 DAVBGY010000000
VERSION     DAVBGY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.068"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(135..1103)
                     /locus_tag="VQ620_001801"
     CDS             complement(135..1103)
                     /locus_tag="VQ620_001801"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET2764382.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1248..2063)
                     /locus_tag="VQ620_001802"
     CDS             complement(1248..2063)
                     /locus_tag="VQ620_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET2764383.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFTNTYIHDLLGVLFPD
                     SPFLNAFESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIG
                     YIRTDLPEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAHKEQKVGKKQGLI
                     FLGLALRTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(2088..2585)
                     /locus_tag="VQ620_001803"
     CDS             complement(2088..2585)
                     /locus_tag="VQ620_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET2764384.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2658..4019)
                     /gene="radA"
                     /locus_tag="VQ620_001804"
     CDS             complement(2658..4019)
                     /gene="radA"
                     /locus_tag="VQ620_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET2764385.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKFAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4033..4548)
                     /locus_tag="VQ620_001805"
     CDS             complement(4033..4548)
                     /locus_tag="VQ620_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2764386.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGFPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4550..4993)
                     /locus_tag="VQ620_001806"
     CDS             complement(4550..4993)
                     /locus_tag="VQ620_001806"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET2764387.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(5050..5147)
                     /gene="ffs"
                     /locus_tag="VQ620_001807"
     ncRNA           complement(5050..5147)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VQ620_001807"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5180..5647)
                     /locus_tag="VQ620_001808"
     CDS             complement(5180..5647)
                     /locus_tag="VQ620_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET2764388.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(5848..7134)
                     /locus_tag="VQ620_001809"
     CDS             complement(5848..7134)
                     /locus_tag="VQ620_001809"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET2764389.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGIGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGARNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(7365..7601)
                     /locus_tag="VQ620_001810"
     CDS             complement(7365..7601)
                     /locus_tag="VQ620_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HET2764390.1"
                     /translation="MLQKIYEQMTNFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            7867..8664
                     /locus_tag="VQ620_001811"
                     /pseudo
     CDS             7867..8664
                     /locus_tag="VQ620_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2818 a         2099 c         1404 g         2460 t
ORIGIN      
        1 tgatgacgta cagtcatatt tcttctcttt ttattatatc acagttttaa atctagcttt
       61 actagattca ccgctactat ctatttattc ggaaaaaaga cgaaaaaacc tgagaatcat
      121 ctcaggcttg gtcattaaat ttttttctca atatcgaaaa gtggagaaag tggtcgtttt
      181 tcatgaatac gtacgatagc atcccctagg agatgagcga ttgaaatctg ctcaatctta
      241 tcaatcaaac gctcttctgg cagatagatg gtatccaaaa caaccaattt cttaatagct
      301 gatttttgga tattgtccgt agcaggacca gaaagaactg ggtgcgtaca gcttgcatag
      361 acttcaacag caccagcttc cgcaagagca tctgccgcat gacaaatcgt tccagcggta
      421 tcaatcatat catcaatcaa gatacaagtc ttgccttcaa ccttaccgat gatgttcata
      481 acttcactag tattcatctt atcaacgcta cgacgtttat caataatagc gatagatgtt
      541 ttcaaaaatt ctgccaactt acgagcacga gtcacccctc catggtccgg gctgacaacc
      601 acatagtcag aaccaaccat accacgacgc tcaaaataat ctgcaatcag aggagcaccc
      661 atcaaatgat ccacaggaat atcaaagaat ccttgaattt gcgcagcatg caagtcgatg
      721 gtcaataaac gatccactcc agctacttca agcatatttg cgacaagttt tgaagtgatt
      781 ggctcacgcg ctctcgcctt tctatcctga cgtgcatacc catagtaagg catgacaaca
      841 ttgacagatt ctgcactcgc acgcttcaaa gcatctacca taatcaaaat ttcaagcaga
      901 ttgtcattta caggcgaact agttgattgt aggataaaga cgtgtttccc acggattgat
      961 tcttcaatgt tgacctgaat ctctccatct gaaaattggc gaacacttga tttccccaac
     1021 tctatcccaa tctcctgcgc cacacgttct gccaattctt tattagaaga aagggcaaac
     1081 agctttaaat cagaaaaaga catgatttcc tccggtatat atgtatcgct tgtgcttttc
     1141 acaagatttt ccatctacca ttgtagcgct ttttgcacta tttttcaatc aaaaataaaa
     1201 gaagggcacc atatttgtac ccttgcatca ttcttttgaa aaatattcta ggtcatcaac
     1261 tcattgtgtt tctcaacaaa gcaataagca tgataaaaac catagagagc aatagccgta
     1321 accactggaa tcgctaaagg caactctgtt tccaactcca caaaaggaga gttaaacaag
     1381 aagtgagttc tcaaggctaa acctagaaaa ataaggccct gtttcttgcc aaccttctgt
     1441 tctttatggg ctctgtaaag caagtaaaca cctactacag ctagacctga aaaagtccag
     1501 tgagaggcaa ttcctgagat gatacgctct aaaattcgcg aaatagtaaa gtcaaagccc
     1561 tctggcaaat ccgtacgaat ataaccaata tcctcaatca tttggaatcc caaaccggaa
     1621 gcaattccaa gtaaaaacaa agattttaat tttcgcacag gaatcaaagc caaaacaaaa
     1681 acaagtggca ataatttcaa gggttcttct accaaaggag ccacaatagc actttcaaag
     1741 gcatttaaaa atggactatc tgggaaaaga acccccagta aatcatggat ataagtatta
     1801 gtaaaactag acaaccagcc tgaaaggaac atccctccca ataaagacag aatcaaaacc
     1861 ttctttggca attcccattt tttcccaata cggaagagaa aataaagagc cggaatcatg
     1921 taaaagagag ctagaaagat agaaactccc attagtccat attccgcacc tgacctcgaa
     1981 ccgtccgtat agtagatggt ttcatactgt aaaccaatac atagcaataa aataaaaata
     2041 aataaaatac tgcttttctt catacacttt ctttctaaat gaagtattta taattctacg
     2101 actgtcatac ttcctgtatc aacattgtaa atggcaccag agataatgac atcgtctggt
     2161 attaggggag actcgataag cagttgcata tcctcgcgta cactctcttc tatatcttgg
     2221 aagggcaaga agtcctggtc tgacacatct acacctaatt cctcttttaa atactcctga
     2281 aaaggttcat tttcaaaggt ctgagcacca cagtctgtat ggtgcaatac cacaatctct
     2341 cttgtcccca tttgttgctg ggaaataact agcgaacgaa tcatgtcttc agtcactcga
     2401 ccacctgcat tccgcaagat atgagcatcc ccaagtgcca agcccagagc ttgcgcaacg
     2461 tgcagacgag agtccataca ggtcacaata gctactcttg ttttgggttt aagtggcaga
     2521 tttaactgcc catgtagggc aacataagcc tgattagctt gcataaactg ttcaaaatac
     2581 gacacgattc cctccttgaa aatttgatag tcaaatattt ctcctatctt atcattttta
     2641 agagaatttg tcacggatta tgcaaagacc tttttcaaaa cttcctgaat cgttgtcacg
     2701 ccaatgacct gaatttcctt aggcagagtg attcctgtca aggaattctt aggtacataa
     2761 atcttagtaa agcccagttt agcagcttcg ttgatgcgtt gctcaatacg attcacgcgc
     2821 cgaatctctc ctgtcaagcc cagttctccg acaaaacatt cctgaggatt agttggcttg
     2881 tctttgtagc tcgaagcaat agcaactgca acagccaagt caatcgcagg ttcatccaat
     2941 ttaacaccac cagcaaattt gagataggca tcctgatttt gcaagagaag ccctgcccgt
     3001 ttttccaaaa cagccataat caagctagca cggttaaaat caagtcctgt cgtagtacgc
     3061 ttggcatttc caaacatggt cggtgttacc aaagcctgaa cctccgccaa aatcggacgc
     3121 gtcccttcca tggttacaac gatggaggaa ccagtcgccc catccaaacg ctcttctagg
     3181 aaaacttgac tcggattgag tacctcaacc aagccgcccg actgcatctc aaaaatccca
     3241 atctcattag tggaaccaaa acgatttttg accgctctca aaatacgaaa ggtgtggtga
     3301 cgctcccctt caaagtaaag caccgtatcc accatatgct ccaacatacg aggcccagcc
     3361 aaggttcctt ctttggtcac atgacctacg ataaagatgg caatgttatt ggtcttggcc
     3421 aactgcatga gttcagcggt cacttcacgc acctgagaaa cagacccctg cacccctgaa
     3481 atctcaggag acatgatggt ctggatggaa tcaataatga gaaagtctgg ctggatacgc
     3541 tccacttctg cacgaacact ctgcatattg gtctctgcat agagataaaa ctcactatca
     3601 atatcaccta agcgctctgc acgtagttta atctgctggg cagactcctc cccactgaca
     3661 tagagaactg tccccacttg ggacaactgg gttgagactt gtaggagaag agttgatttc
     3721 ccaatcccag gatccccacc gatgaggacg agacttcctg gtaccactcc gcctccaagc
     3781 acacggttga attcctccat ctccgtctta gttcgattga cattgatgga agtcacctca
     3841 gctagtttca tgggcttggt tttctcacct gtcaaggaca cacgcgcatt cttgacctcg
     3901 gcaacctcaa cctcttccac aaaagaagac caagacccac agttggggca acgtcccaga
     3961 tatttaggag aattataccc acaattttga catacaaatg tcgctttttt ctttgcgatg
     4021 acaaacctct ttctatatct ctaactcaca ctcaatcact tggcaaaaat caatcttctc
     4081 atttggcaca aactggcgca tgagcattcg atgagcaaca actaccacag tctgatgttc
     4141 tcgatactta gacatacatt ctagaaaccg agacttcatt tccgtagctg tctcatattg
     4201 aataggacta ttaggaagca actccccctt gttttctaaa aacagtcttc tagctgtttc
     4261 aaagttttct attcctgttt tatagacctg ccattcatgt aataaaggct ctactcttaa
     4321 aggaaaaccc gtagcacaga ccacatacga agccgtttct aaagctcttg tgactgcaga
     4381 agatacgatt atttcagctg acgagagtaa aggatttttg ctcaatttct ggacttgctg
     4441 ccgtcccatc tcagacaagg gtgccaaatc tatcccaaat cctatataag aacgctcctc
     4501 taactcacgg taatctggct ccccatgacg tacaaagata atcttcattc tagtgccctg
     4561 tcgatccaaa tccaccagtt cgaacgccat cagccgcatc tccatctgca attaagaaag
     4621 tagcaaaaac agcctggaca atacgctccc caacttcaag aacaacctct tggtctgtga
     4681 tattcttcat ctgcgcaaaa atatgccctt catttccagg atttccataa taatccccat
     4741 caatgactcc aactgagtta attaaaacca agcccttctt acgaggattt gaagaacgat
     4801 catagaggta gagaacctca gtcggctgca tataagcctt aacccctgtc ggaaccaaga
     4861 caatctctcc tggcgcaaca actgtacgca cagcaacctt taagtcgtaa ccagccgcat
     4921 gcgctgtctc acgcttgggc aataaatttt catctgtaaa actcgaaacc aattcaaaac
     4981 cacgaatttt cataattttc tcttttctat tatcatttat tctagattat tctatcttat
     5041 ttattcggaa aaagcacgaa aaaaagagca cacaattcaa atcgcttagg gctgctggat
     5101 tcctcccctg acccgcttca cgcagaactg ttgctccact atttattata tcacattcct
     5161 attcatttta aaagcaaaat tattttttcc gtctatttct aaaaaaatcc tgcatgatag
     5221 ctgcgcattc atcttccaaa attcctattt caacctccac acgatgatta agacgctcat
     5281 ctgtcaagat atcgtacaaa cttccagcag cgccaaattt ctggttttta gccccataga
     5341 ccacatttgg aatgcgggca agcccaatcg ccccactaca catgacacaa ggttcaatgg
     5401 tcacaaaaag tgtgcaatcc agcaagcgcc agctctcctc actcaagttc gcatcctcta
     5461 tagccataat ttccgcatgc ataaccgctc gctgtaattc ctcacgcgca ttatgcccac
     5521 gaccaatgat ttccccatct ttgacaatca cacaaccaat tggaatttca tcgtgttcaa
     5581 gagcaatctc agcctctctc aaagcctccc tcataaagac ttctttttct tcaagcgtat
     5641 aatacatctc ttttctcttt tcctacttat cgattttatt attatatcat gaatcccaac
     5701 acaaaaaaag ccaccgaatg cggtgactct atagggagat tattatgaaa aagaaaagtt
     5761 taggatattt gttacaacaa gttaggaggt cttcttgtaa ctgtctatag tatacccgac
     5821 ctatcttaaa caaatcttaa aaatctctta ggaccaaaca ctttctaaaa tatttgtttg
     5881 ttcacgacca ggacctactg agaaagtaga aatacgaacg ccaaccaatt cactcacacg
     5941 acgaacatag ttacgcgcat tctcaggaag atcttccaaa ttgcgagctc cggtaatatc
     6001 ttctgaccaa cctggcaact cttcatagat aggcttgcaa cgtttcaatt gctcaagact
     6061 agctggatag tagtcaatac gttgaccgtc aagatcatag gccacacaaa ttttcacagt
     6121 atccaagccg ctcaaaacat caatagagtt caaagaaagg ttagtaatac cagaaacacg
     6181 acggctatga cgcatcacaa ctgagtcaaa ccaacctaca cgacgtggac gaccagttgt
     6241 tgtaccatat tcatgaccca cttcacggat acgttctccc acttcatcaa acaactcagt
     6301 tgggaaagga ccatctccta cacgactcgt ataagcttta catacaccta caaccttgtc
     6361 aatcttgctt ggaccgatac cagaaccaat tgtcacacca ccagctacag ggtttgatga
     6421 cgtaacaaat ggataagtac cttgatcgat atctagcata acaccttgtg caccttcaaa
     6481 aagcacacgt ttgccattat caagcgcatc attcaagata acagatgtat ctatcacgta
     6541 tttcttgatt tgttgaccat attcgtaata ttcttcaaaa atatcatcga aaacaatcgc
     6601 tttactgtca tacaattttt caaaaagacg attcttttca gcaaggttac gttctaaacg
     6661 ctcacggaaa atatctttat ctaaaagatc tgcaatacga attccaacac gagcagcctt
     6721 gtccatataa gctggaccaa ttcccttaat tgtcgtacca atcttattgt cgcccttagc
     6781 ttcttcttgc aagcgatcca actcgatatg ataaggcaaa ataacatgcg cacgatcaga
     6841 aatacgcaag ttatcagttg ttacaccttc ctcatgaaga tagctcaact cttttacaag
     6901 agatttagga tttacaacca taccattccc aatgacagat attttttcag ggaagaaaat
     6961 cccagatgga atcaagtgca acttaaattt cttaccgtca atcacaatcg tgtgaccagc
     7021 attatcacca ccttggtaac gtgcaattac ttctgcattc gctgaaagga agtctgtaat
     7081 cttcccttta ccttcatcac cccattgggt acctacaaca acaactgaag tcataatttt
     7141 gtctgagccc tcaggctctt cctttctcac atacatggca ggactctcac ctgcaattat
     7201 atcttacaat ttattataat aaaaaatcgc ctttttatca agaagaaaca atagaaagat
     7261 ttgctatttc caactattaa aaaatgattt agaaaaatta ctagctattt actattatct
     7321 ttccataaaa gagtaaatta gttcggaaat ttactaaaat tacctcaaca agaaataaaa
     7381 ccccgattca ttaccaattt ttcaagatac aaacgataag caacacgata atggtaaacg
     7441 ataaaatccc tacgacaacc aatgccatat ctcactaaat aataaattaa aaatttaaaa
     7501 tgaacatgtt cccagtcaaa attatcacca aatgtaggac catactcttc ttcaatacta
     7561 tcatagaaat tagtcatctg ctcataaatt ttttgtaaca taatcaaata ctccttttct
     7621 tttttataaa cttattctaa caaaaaattt tacacattca ctatcaattc ctgaattgtt
     7681 aaaataccag cctcaacaag ataaaaatag gaaaagttga caaaatagaa acaaatttgc
     7741 tacctaaatt tcaaacgatt aaagttctaa aaagaccgcc ttaaaacttt tgggtaaatc
     7801 ctattagaac tataatattt atatatatgt attatggttc tgattttcta gtataaaaaa
     7861 attcaaacta gaattcttct tagaaaaggc cgttttaagt gctatcattg ctcaaaaatg
     7921 gaggtcgctg agacttctat cgtcaagaag aatcatcaaa ttccttgtat catcaaccaa
     7981 aagattgctc agaagttaat tgaaaagact tctatgaccg acattgatca tcagttgtct
     8041 atttcaactt caactgtcat tcgcaagatc aataattttc actttgagca tgatttttcg
     8101 cgtcttcctg agattatgtc ctaggacgtt gaaacagtcc ggggagtgac tgtttcaatc
     8161 gggagatgga gatgagcttt attgctcagg actttgataa actcaatatc ataactgttc
     8221 ttgagagcag aacacaagcc atcatccgaa atcccatgaa tacaaggcta tcaagcgcta
     8281 ctggaagctc attcaacaag atagtcagaa actgagtgat aaacgatttt atcgccctac
     8341 ttttcgtatg cacttgacta ataaagaaat tctagataaa cttttgagct attccgaaga
     8401 cttgaaacac cactataatc tctatcagct cttgcttttc cacctctgtt tcagactttg
     8461 tttaaaacct tcctcaaaga taaagagaaa atcgtcaacg cccttcaatt accttattcc
     8521 aacgccaaac tggaagccac caataatctc atcaaactta tcaagcacaa tgcctttggt
     8581 tttaggaact ttgaaaactt caaaaaagaa aggacgaaat ttgtcctttc tagatcttcg
     8641 ctttcttcaa cccactacag ttgacaaaga accgaaaaat gtctcgcctc taatatagtc
     8701 aattgaaaca agaacaagag aaaagagcct cgtaaaaggt attgcaactt ggtaatacct
     8761 ttttgaggtt ctttttgata t
//
LOCUS       DAVBGY010000069         8751 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVBGY010000069 DAVBGY010000000
VERSION     DAVBGY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8751
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.069"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(60..1163)
                     /locus_tag="VQ620_001812"
     CDS             complement(60..1163)
                     /locus_tag="VQ620_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET2764391.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDNLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            complement(1180..1977)
                     /locus_tag="VQ620_001813"
     CDS             complement(1180..1977)
                     /locus_tag="VQ620_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET2764392.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            complement(1964..2641)
                     /locus_tag="VQ620_001814"
     CDS             complement(1964..2641)
                     /locus_tag="VQ620_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HET2764393.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            complement(2743..5079)
                     /locus_tag="VQ620_001815"
     CDS             complement(2743..5079)
                     /locus_tag="VQ620_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET2764394.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGNVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICISSALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GSSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTGQTLPVYGVMMLVFTVIFILTSRYQAKK"
     gene            5212..5961
                     /locus_tag="VQ620_001816"
     CDS             5212..5961
                     /locus_tag="VQ620_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET2764395.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            complement(6254..6868)
                     /locus_tag="VQ620_001817"
     CDS             complement(6254..6868)
                     /locus_tag="VQ620_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HET2764396.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(7602..7871)
                     /gene="rpsO"
                     /locus_tag="VQ620_001818"
     CDS             complement(7602..7871)
                     /gene="rpsO"
                     /locus_tag="VQ620_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET2764397.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(8012..8641)
                     /locus_tag="VQ620_001819"
     CDS             complement(8012..8641)
                     /locus_tag="VQ620_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET2764398.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
BASE COUNT         2548 a         2023 c         1681 g         2499 t
ORIGIN      
        1 cagtgctttg agtaatccgc ggctagtttc ctagtttgtt ctttgatttt cattgagtat
       61 tattcgcttt ttactcgttt gacatagttt tcaattgggt aatttagagg gtccaaggtc
      121 aactccttgt cttggatcag ttgggctaga tggtaaccaa tgataggacc agttgtgagg
      181 cctgatgaac ctagtccact ggctgcatag acacctgcca attctggcac ctgcccaaag
      241 aagggagaga aatcactggt ataggcacgg attcccacac gctcagccct tgaagtcact
      301 ctagctaaaa ctgggtaatg aggcaaggcc gcctcctcca tttgttggag caagggttca
      361 tctaccgtca aatcaaatcc catgtcattt tcatgggtag cgcccaagga taatttccca
      421 cctgcaaagg gaatcaaatc ccactcccct tctggcatga caacagggta atcttccatg
      481 tcttgggcaa gctgataatc tcgtagttgt cctttttgag gacggacatc cacttcataa
      541 cctaaaggtt ctaacatgtc ccccaaccaa gctcccgttg ccaaaataac ctgatcaaac
      601 acctcttcgc caatctggta gcctgatgat aagagtgtca gagtcacttt ttctttgacc
      661 aacttgacat gactggcttc tagcagacga gttaccaaga gttggccatc tactctcgct
      721 ccaccagaag catagagcaa gttgtcgaat ccctgcaagc cagggaataa ttcattagct
      781 gatgcttgat ccagaatggc taactggcct atcaggggag attcttctct gcgctggaga
      841 gccagttgat agagctcttc caacttggat tcatcctttt tcaagagaaa gactcccgaa
      901 cgctggtaaa agtcgatttc ttgtcctgat ttctctaaat cagctaataa atccacataa
      961 aaatcagccc ccaagcgcgc catcttgtac caggctttat tacggcgttt ggaaaaccaa
     1021 ggactgataa ttcctgctgc ggccttggtg gcttgacctt gctcatggtc aaaaacggtc
     1081 acctctaggt cactttctct cgagaggtag taggcagctg ttgctcccac aattcctgct
     1141 ccaataatgg caactttttt cattgtcttc actttctaac tagatatgac ggaaaggatt
     1201 ggtcgatgct tgactaggta aaatctctag ttcccagcct tttttctctt tccattgagt
     1261 aaggagtgct gcgatttttt ccacaaaaag cacttcgata tagtgacctg gatccaatgc
     1321 taacaagcca tcagacagca tatcctgagc agtatggtag tagatgtcgc cagtgatata
     1381 gacatctgcc ccctttgcca aagcatcctt atagaaagac tgcccgcttc caccacaaat
     1441 tgctactctt gaaataggct tctgcaaatc attctcttga taatgcacca ttcgaaggct
     1501 atctaggtca aagacttgct tgacctgttg ggccaattcc caaaatgtct gaggctgaat
     1561 attcccaata cgtccaattc cacgttctgg acctgtttcc tgcagataag tcgtctcctc
     1621 gattcctagc atctgacaaa accagtcatt gagcccattt tcaacgatat caatattggt
     1681 atggctgaca taaactgcga tatcatgctt aatcaggtcg atgtaaatct gattttgcgg
     1741 acggctggca agcaagtcct tgataggacg aaagataggc gcgtgcttga cgataatcaa
     1801 atccacaccc ttttcaatgg cctctgccac cgtctcttca cgaatatcga gggcaaccat
     1861 gaccctttgg atacccttgt ctaaagtgcc aatttgcaga ccacggctgt ctccctccat
     1921 agaaaattcc tgagggcaaa aggcttcata agcttggatc acttcacttg ctaacatgga
     1981 gcacctcctt gatagcttga atcttatcta ctagaacttg acgttcttcc agattttttt
     2041 ctgggatttg tccgagggcg aactctagct tctcagcttc tttttgccat ttttggacaa
     2101 atactggact gacttctttg gacaagaagg gaccaaagcg aacatcactg gctgatagct
     2161 tcatttgtcc tgcttccacc accaaaatct cataaaactt tccagcttct tctaagatgc
     2221 tttctgctac aatctggaat ccatgatcct gtagccagat acgcaagtcg tcttcacgat
     2281 tattgggctg gaggatcaaa cgctctacat tagctaactt ccccaaacct tcttctaaaa
     2341 tcctagcaat caaacgacca cccatgccag caatggtaat gacagacact tggtcagtct
     2401 cttcaaaagc tgccaagcca ttggctaaac ggacttggat tttctccttt aggccgtgag
     2461 cctcaacatt tttaaccgca gactgatagg gaccttccac cacctcacct gcaatagcgc
     2521 ttttgatttg gcctctctca accaactcga taggcagata agcatggtca cttcccacat
     2581 ctagtaaaat agccccctgt gacacaaagg aagctaccaa ttctaatctc tttgaaatca
     2641 tcttctctca ctttccaaaa ctctattacc tcttattata ccacatttcg gctggcaatc
     2701 ttgtacctaa aaaaaccgca atccacagat tgcagtcttt ctttattttt tagcttggta
     2761 acgactggtc aggatgaaaa tcacggtaaa gaccaacatc atgacaccat aaacaggcaa
     2821 cgtttgtcct gttaaggttg aaatttcaag cagtttttgg attcttggga agagagctgt
     2881 ggctaggaaa cctcctactg accaaacaat caagaggaca cgccataggg taaatggcat
     2941 gcaggctcta aatacggata agaaaccaat tgaccccaag agataataga gtagagttga
     3001 gatttctaac tcagaccaac cttggctact cccaaatatt ttcacaaaca ggacgctgaa
     3061 gacgaccatg agagcgcttg gtagggcacg aagcatggat tttctgagga aatttggctc
     3121 aacaggtttg atatttcgct caaaagtcag aacgaatggt gggaaacctt ccacaaactg
     3181 gtcaatcatg gtaatctgga ttggaatgaa ggggaaaatc aagatccatt cagaccgacc
     3241 tagtaaagca ctggagatac agataactgc gagcaagaat gagtagatgg tctttatcaa
     3301 gaaaatcggg gcgatgtggg caatgttatt gaccacgcga cgaccctcga agagaatctc
     3361 aggaacatca ttaaagtctg agttcaagag aaccagattg gcaatctgac gggttgctgg
     3421 atctccctcc gccatcacga tagaacaatc cgcctcacga agggccaaga tatcattaac
     3481 cccgtcccct gtcatagccg ttgtatgacc cgcttttttc aacgtttgga tgatgagttt
     3541 cttttgatga ggggaaacac gtccgaaaat agctgtctcc tccgccatgg ccatcaattc
     3601 ctcatcggtg atttttgagc aatctacata gctgtgatag tccgcaaaac cagccttctg
     3661 ggcaatgctg gacaccgtaa ctggattgtc accagagata atcttgagtc ccacctcctg
     3721 agaacggaga tagtccagcg tctctgctgc tccctctcga atggggtcca agatttccag
     3781 caaggctaga gcctgaatat cagatggttt ctgtggtttg tgatggtcta atttctcctg
     3841 actgagagct aagaccaaga cacgtgatcc tctctccaag gcctccctgg cttctgggac
     3901 ttcagaatcc agcaacatct caggtgcccc taagaaaact gtccccaagc cttctaactc
     3961 catagccccc cacttgcggt cgctggagaa gggaagattg gaaatcatag gataagcaac
     4021 atttcccaca aaacgctggc gaatggcttg ggcagttgga ttcttatcct cactatgggc
     4081 catgtagcta gtcaagatgc tggcaatagc ctcttcaccg taagtttccg tcagtggaag
     4141 aacagcctcc acctgcatct ttccttgggt gatggtgccc gtcttgtcca gacagagcat
     4201 atccacgcgc gccaaggtct caacagagta catctcctgc accaagacct ttttcaagcc
     4261 caacttaatc actgcagtca agagcgaagt aatggttaaa agggcaattc ccttaggcaa
     4321 cattcccaaa agagctgtcg acgagtttac aacggatgac ttgagaggca ggccttttaa
     4381 aagcaaggct tccagcaaga gagccagacc aaagggaatg ataatcttcc cagtaaaacc
     4441 agccaacttg tccagcgatt tcatgatacg ggagttgatg ggtttaacgg tcttagcctc
     4501 aagcatgagt ttggcagcat agttgtctgc accgacatgg tgaacttgag ataaaactga
     4561 cccactggct aggaaacttc ctgacaaaag taagccgtca acttcctttt gcaccaaatc
     4621 actttctccc gttaacatgg cttcattgac ttccgcaaag ccttccaaaa ccaaggcatc
     4681 actaggaatc tgctctcctg cagacaaacg aatgacatct cctagcacta attcttcagg
     4741 attaagagca acttcctgac catcacggat ggttttgacc ttttccttgg tcatgagatt
     4801 gagcttgtcc accatgtgtt tggctcgtag ctcggtcaca atcccagaaa aagcgttaaa
     4861 gcagataaca gcaaagaaga ccagattgct ccaagcctgc acaaaggcaa gggccaaagc
     4921 aatggcaaag ttcaaagcgt taaaaagggt aaagacattt cgtttaacga tttgccaagt
     4981 gctggtactg gctgatgcgg taaagtcatt aaccaaaccc tcagcctgtc tttccttgac
     5041 ttctctttgg gttaatccca taatcttatt tttatccata aattctatta tatcattttt
     5101 tttaacagaa ttctaatttc atcccaaata tgcatctagt caagggaaaa gtttgccagc
     5161 cctcctttga taaggtataa taataacaag aaaacaatcg aaggagatcg catgttttat
     5221 acttatttgc gtggattagt tgtattgctc ttatggtcca tcaatggcaa tgctcactat
     5281 cataatactg ataaaattcc taatcaagat gaaaattata ttttagttgc gcctcaccgt
     5341 acctggtggg atcctgttta tatggccttt gcgaccaagc caaaacagtt catctttatg
     5401 gcaaaaaaag aactctttac caaccgtatc tttggttggt ggattcgtat gtgtggcgcc
     5461 tttcccatcg accgtgaaaa tcccagcgcc tcagccatca aatatcctat caacgttctc
     5521 aaaaaaagtg accgctctct catcatgttt ccaagtggta gccgccactc aaacgatgtc
     5581 aaggggggcg cagcactgat tgccaaaatg gccaaggtcc gtatcatgcc ggttacctac
     5641 accggtccca tgactttgaa gggcttgatt agccgtgaac gtgtcgatat gaactttgga
     5701 aatccaatcg atatctcaga tatcaagaaa atgaatgatg aaggcattga aacagtcgcc
     5761 aatcgtattc aaacagaatt ccaacgtctg gacgaagaaa cgaaacaatg gcacaatgat
     5821 aaaaaaccaa atccactctg gtggtttatc cgcatccctg ccctcatcct tgctattatc
     5881 ctcgctatcc taaccatcat ctttagcttt atcgcaagct tcatctggaa cccagataag
     5941 aaaagagaag aacttgcata gaagaaatga accttggcca aacagctaag gttttcattt
     6001 atatatcaaa ttgaaactag aatagtacac ctctacttct taaaacattt ttagaaatcg
     6061 atttgactgt cctgatcgat ttgtcctgtt tttgtttcat tttactatat ttataagaat
     6121 ctcctaatca aatcaaaagc cactctacaa tagagtaact ttaagattgt ccatttatac
     6181 tcaatgaaaa agccctctga aaatcagaga gctgatttga gctcgggcta aaatcctagt
     6241 gaaacaaaaa aatctacacg atcagaaaag tctctatcgt gccattttca tacatgatgt
     6301 atagtccaag tagaatgaat actaagggca caatgattcg ctcgtatttt tcaattgtct
     6361 cgaatattaa cggaatagag gataacaccc gactaatctc gcaaaagata attatgccga
     6421 ttacaaacac aagcaaggcc acgagggtct gtgaccaatc taacgaagca aaataaggta
     6481 tatagatacc taaattatct ccgccagacg caattgtcag caatgtaact gtccaaaaca
     6541 gttgatttgc cttgctttgt tctaatcttt caataatttc ttcctcttct tcttccgcat
     6601 cttctccaac aattgcaaag cgaatcccta aatagatagg gattaaacca agcaatccaa
     6661 ccatccattc ttcaggcacg aaattaacga cataagcagc aactaaactc gcccctacaa
     6721 gtaagcctgt gcctagatat tgccccgcat aaatatgcca tttctgttta ttctgtgata
     6781 gctgtgcaaa taaaataatt aaaataatta aataatcgat actggtggaa atataaacac
     6841 caatagcaga tatgattgtc tgtcccataa aaaaaactcc tgtattagtc agtaataaat
     6901 cagcagattt taggagagca cagacacatt aatttagcta tcgctagtgg gtaatgtcga
     6961 acgtaggaaa gcattttact cctcctcaaa acgtagttac aaagtaattt ctaactggaa
     7021 ttcagtgact acttccatgt aaagtataac atactatact ttactttgta aagagtgggc
     7081 tcttctaggg ggcttgcata cagctactcg gcttgaaaag ccgagtaact gcgcaccttt
     7141 atggtgtaat tagctgatac cgtttgaggt ttttgtagtc tccaaaattg ttaccgataa
     7201 aacctcaata aaaaagccct ctgaagtcag agagctgatt tgagcccggg ctaaaatcct
     7261 agtgaaaaag atgaaactcc ttgtgctcat cgaacactgt gtcatttccc tattttcata
     7321 cggatttttg acgcccttag catcatgatt cttgctagat aaatcgttta tagactaggc
     7381 gacgaggcga agatcgtacg aaactttcag tgaaacaaaa aaaatctccc cgaagggaga
     7441 aaagctggtt tattttacct tacagtgcta ggaaccgtaa ccaaagatta tacttgagta
     7501 tagcgaggta aggtgacaac gcaaaaaagc tacccaattt agagtagctt cattatcaag
     7561 atatgctcaa ttgaacacgg cctaagcact tgtatcttga attagcgacg aagtcctaga
     7621 gagttgatca actcacggta acggttaacg tcgtttttac gcaagtatgc aagcaagtta
     7681 cgacggcgac cgattttttt catcaatcca cggtaagtag cgtggtcttt tttgtgttgt
     7741 ttgatgtgtt cgttaaggtg gttgatttcc caagtaagga cagcaacttg aacctctact
     7801 gaacctgtat caccttcgtg acgtgcatat tgtgcaatga tttcattttt tttctctttt
     7861 gagattgcca tgatgtttct cctttttatt tggcttcatc cgagtgacag gttggcatgc
     7921 ctgtaaccaa gaagaagtta tttgtcttta cgacaatccc tattttacca agatttaaat
     7981 tttctgtcaa acattaatac tctcatagtt attatttttc aagtaaagag atacgttctt
     8041 ttatatgatg acctttttca agttcatcta ccaatccaat agcataatct gcataactaa
     8101 tctggcttat tcctttttca ttagtagtaa aaatttctcc tgctagaatg tagtttcctg
     8161 ttttttcacc atcgggaata aaatctacag caggactgac aaaagtccag ttgagattat
     8221 tttttgtacg aagaagatcc aattcatccg cttgagcttt tgctagtgac ttaaattctt
     8281 caggaaaatc agacgtatct aaaagtcgtg tcgtttttgt ttcatctata taaagacttc
     8341 ctgctccccc tacaactaga aaacgtgtct gtgttccagc aagaagctga ttaaatagtt
     8401 cgattgattt gctgtggagc ggtagcgtat ctggtgtata agcaccaaac gctgaaataa
     8461 cagcatcaaa tccagtaaga tcatcttttg tcaactcaaa taaatctttt ttaataatag
     8521 actcagcttg acttttgttt tcagaacgaa caatagccgt tacttcatgt cctcgtttga
     8581 ctgcttcttc aacaattgct ttccccgctt gtccatttgc tgcaataact gctagtttca
     8641 ttttttatac ctctcttgtt gtaattattt tagttacaac aattgtgaca ctcttaataa
     8701 ttaatgtcaa tagtcttgct taattattat caaaatattt ctaccaagaa a
//
LOCUS       DAVBGY010000070         8593 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVBGY010000070 DAVBGY010000000
VERSION     DAVBGY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8593
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.070"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            218..1408
                     /locus_tag="VQ620_001820"
     CDS             218..1408
                     /locus_tag="VQ620_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HET2764399.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(1729..2610)
                     /locus_tag="VQ620_001821"
     CDS             complement(1729..2610)
                     /locus_tag="VQ620_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HET2764400.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(2751..4079)
                     /gene="vncS"
                     /locus_tag="VQ620_001822"
     CDS             complement(2751..4079)
                     /gene="vncS"
                     /locus_tag="VQ620_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HET2764401.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGATMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(4076..4732)
                     /gene="vncR"
                     /locus_tag="VQ620_001823"
     CDS             complement(4076..4732)
                     /gene="vncR"
                     /locus_tag="VQ620_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HET2764402.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALDQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASIAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(4781..4864)
                     /gene="pep27"
                     /locus_tag="VQ620_001824"
     CDS             complement(4781..4864)
                     /gene="pep27"
                     /locus_tag="VQ620_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HET2764403.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            complement(5034..6413)
                     /gene="vex3"
                     /locus_tag="VQ620_001825"
     CDS             complement(5034..6413)
                     /gene="vex3"
                     /locus_tag="VQ620_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HET2764404.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(6465..7112)
                     /gene="vex2"
                     /locus_tag="VQ620_001826"
     CDS             complement(6465..7112)
                     /gene="vex2"
                     /locus_tag="VQ620_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HET2764405.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(7125..8402)
                     /locus_tag="VQ620_001827"
     CDS             complement(7125..8402)
                     /locus_tag="VQ620_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2764406.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITSYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         2434 a         1881 c         1630 g         2648 t
ORIGIN      
        1 acaacctcaa aacggtgttt tgagcagcct gcggctagct tcctagtttg ctctttgatt
       61 ttcattaagt ataaacagcc actctccagt tcaattggag agttttttga tgagagtaga
      121 tatttataga cctcaatttg acaccataca ttttctatga taaaatgtaa caaattcatt
      181 tacaacaaat cgaaatctaa atgaaatgga attttctatg aaatcagtca aaggactact
      241 ctttatcata gctagtttta tcttgactct tttgacttgg atgaacactt ctccccaatt
      301 catgattcca ggactagctt taacaagcct atctctgact tttatcctag ccactcgtct
      361 cccactacta gaaagctggt ttcacggttt ggagaaggtc tacaccgtcc acaaattcac
      421 agcctttctc tccatcatcc tactaatctt tcataacttt agtatgggcg gtttgtgggg
      481 ctctcgctta gctgctcagt ttggtaatct tgccatctat atctttgcca gcatcatcct
      541 tgtcgcctat ttaggcaaat acatccaata cgaagcttgg cgatggattc accgcctggt
      601 ttacctagcc tatattttag gactctttca catctacatg ataatgggca atcgtctcct
      661 tacatttaat cttctaagtt ttcttgttgg tagctatgcc cttttaggct tactagctgg
      721 tttttatatc atttttctat atcaaaagat ttccttcccc tatctaggaa aaattaccca
      781 tctcaaacgc ttaaatcacg atactagaga aattcaaatc catcttagca gacctttcaa
      841 ctatcaatca ggacaatttg cctttctaaa gattttccaa gaaggctttg aaagtgctcc
      901 gcatcccttt tctatctcag gaggtcatgg tcaaactctt tactttactg ttaaaaattc
      961 aggcgaccat accaataata tctatgataa tcttcaagcc ggcagcaaag taaccctaga
     1021 cagagcttac ggacacatga tcatagaaga aggacgagaa aatcaggttt ggattgctgg
     1081 aggtattggg atcaccccct tcatctctta catccgtgaa catcctattt tagataaaca
     1141 ggttcacttc tactatagct tccgtggaga tgaaaatgca gtctacctag atttactccg
     1201 taactatgct cagaaaaatc ctaattttga actccatcta atcgacagta cgaaagacgg
     1261 ctatcttaat tttgaacaaa aagaagtgcc cgaacatgca accgtctata tgtgtggtcc
     1321 tatttctatg atgaaggcac ttgccaaaca gattaagaaa caaaatccaa aaacagagct
     1381 tatttacgaa ggattcaagt tcaaataagt aatcatactc tcctgggatt tcccaggaga
     1441 gttttttata gccaaatgaa ataaaaccgg aacgatggaa ttttggcatt caaatcaatt
     1501 gataagaata ttttatagtg gagtgaaata ggatacgaac aaattgatta ggaaaatcaa
     1561 atgaatttat agaaatcttt tagcagttat gttgtcctat tctagtttca aaacgctata
     1621 gaagcagcat tgtgctagtc tagattcagt ttactatact aaaacgagta gcttgaaatc
     1681 aaaaaaccca ccctcacagg caggttttat ctgtattatt cagctagatt atgctttacc
     1741 ttctgaaccg aatacgtcga tacgttcttc aaccgatgct tggatagctt ttacaccgtc
     1801 agccaagaat ttacgtgggt cgaagagttt tttcttgtcg tattctgctt cgtttgcttc
     1861 gtagtcacga gcaaatttac gagttgcgtt agcgaatgcg atttggcatt ctgtgttaac
     1921 gttaactttg gcaacaccaa gtttgatagc tgcttggatt tgctcatcag gaatacctga
     1981 tccaccgtgc aatacgattg ggaatcctgg aagagcttct gtcaattttt gcaagtggtc
     2041 aaggtcaaga ccttcccagt ttactgggta agggccgtgg atgttaccga taccagctgc
     2101 caagaagtcg ataccagttt caaccattgc tttagcgtct tcgattggag ccaattcacc
     2161 tttaccgatg attccgtctt cttcaccacc gatagtacca acttcagctt ctactgagat
     2221 acctttagcg tgtgcttttt caacaacttc tttagccaat ttaaggtttt cttcaactgg
     2281 aaggtgtgaa ccgtcaaaca tgattgaagt ataaccaact tcgatacact caagtgcatc
     2341 ttcgtagtga ccgtggtcaa ggtggatagc tactggtaca gtgataccca ttgattcaac
     2401 aaggttagcg atcaagttgc gagcaacttt gtaaccaccc atgtatttag cagcacccat
     2461 tgaagtttgg atcaaaactg gagctttttt agcttctgct gcgcgcaaga tagcttgagt
     2521 ccactcaagg ttgtttgtgt taaatccacc aactgcataa ccgttgtcac gggctgcttg
     2581 gacaaatttt tctgctgaaa cgattgccat tttatcaggc ctcctgtata tttttatggg
     2641 tcatcccatt tacattgttc attttatcac tttttgccaa aaaaatctag tttttcccgc
     2701 agtttcgatt gattttcttc taactccatc tatgtaaacc ctttctctcc ctagtcttgg
     2761 acgacttttg gaaaatctat aaagaaggtt aaacgattct cctccatctc gaaacgataa
     2821 gctaattttt catgttctaa tagactctta accacaaaga gccccatacc agaccccttg
     2881 accttgcgac tggcattgtc agaaaaagac tgggctagtt tttcttgttc ctctgagcta
     2941 cagctatttt cgataaaaag ttctccttct ctttctccaa ttcgaactaa gccacctgga
     3001 acagagtgct taatggcatt gctgatgaga ttagaaagaa tcaacttcat aactgatggg
     3061 tttagataag cctgctgatg ggtcaaacta ttgtctatct ggagctctct ttccttggct
     3121 agcaaggcat aatctttgac cagattttgc gtcatctgga ggaggtcaat tgtttcccta
     3181 tcatctcgca attcctgcac agaagagagg gaaagtatct gcagaacatg gtgattgagt
     3241 tcatccacaa tccccaaggc aactcccaga tactggtctc tatccttata acgaccgata
     3301 ttctctctca tattttcgat taggattttc aaactagcca gcggtgtttt caattcatga
     3361 gaagctcctc gtaggaattc gaccttcatc ttctccagct ggagaatggc ttcattcttt
     3421 tcatgcaagt ccgcaataac agtcaagaga tgctggtaga ggctattgat ttgttccttg
     3481 agattaccta tctcatcctt agaatccacg cgcaatcgca cttgggaatc caggtccatc
     3541 atccgacggg tcacccgctt gatttccaag atcggtgcaa caatagtccg agcgtagatg
     3601 taggccacca agagggaaat cagaaaggag gccagcaagg tatagggaag aaactggaga
     3661 ctgatttgct ccgcttcctt ttgtaaatcc atggaagcta gaaactggag aatcatagta
     3721 gcaccgtctt gcgttttcac ctcgcgctcc tcaataaaga gagaggttgt ctggcggtct
     3781 gtgtccagag gaagactgtc cttgacttct aacttgtcct cggtcatctc acctttgacg
     3841 gtccccttga tatcactagt ctgggaatac aagtctaaca cttgctcgat actctgccta
     3901 tctttccctt ctagggactg ggcaatggct gttgcctttt gaccaatggt ttcctgacga
     3961 tgactcagat aagtcgaagg aaaaagaaaa taaatagcta aatgaaggca gataaccaga
     4021 acactaaata tcgagaaggt atagataaat atctttgcaa ataaacctgt tcgtttcatt
     4081 ttcgctccaa tttataacca acattgcgca cagtgaggat acaatccaag tctagctttt
     4141 tccgcaattc cttgatataa acatcaataa cacggtcaaa gggaacctca tctgtcgctt
     4201 tccagacggc atcgataatc tgagatcgag tcaaggcccg gccttcattt ttcactagat
     4261 agtccagaat ttccaactct ttggcattga taggcacttc ttgacctgcg atgcttgcac
     4321 tgtagctttc aaagtccacc ttggtatcct tgtaagaaaa gattcgtcct gtatcgtagt
     4381 agcgcttgaa aatcgcgtcc accctcactt ttaaaaggga gagggagaaa ggtttttcca
     4441 gatagccatc tgccaaagag gcaaaggcac tcatcttgta ttcctcatct tgaaaagctg
     4501 tcaacatcaa gacaggaacc tgactggttt tacgaatctc agctaggact tctaagccgt
     4561 tgagcttggg catctggata tccagtaaaa ccagggccac ctcatagcta gaaaattgat
     4621 ccagagcttc ctgaccgtcc gctgcctcaa tagtttcata gccacaatcc gtcaaataat
     4681 cactgacccc ctcacggatc atctcttcat cttctacaat taaaattttc atactttaac
     4741 tgctctctat tttttatttt tcttagaata aatacctact ctattttcta ttatagtctc
     4801 ttgctggcct tttgtctgca agcaactgac cactagataa aacgttgtga aattcctttc
     4861 tcataaattc cataacttta gtatattata tttaagcact aaagtacaaa gaaagcaatg
     4921 attttcacca ctgctttcgg atttattttg aattgttaaa tagccattcc tatccactat
     4981 tcttgaatag aaacacaaga tgcaatcttt attctagact cattttttca aatttattca
     5041 ccatccagca agagctcttt tggttgtttt ctaaggagat tgcttgaagc aagcgccata
     5101 acgagaacca ctagaaccaa ggcaaggaca aaaatgatga taaagtctga tgtctgaatg
     5161 gaaatgtcta ggctcgacaa ggtcttgcta aagccatcta cttctgcacc accaccaagg
     5221 ttagaggctt gagccgcctt actagcctgt ttggcaacac ctgaagtcac attggcaagg
     5281 acagtgtttc caattgcacg ggcagtgtaa ttagctagga agtaagcaga aactagagca
     5341 gggatagcaa tcaagataga ttcggtgatg aattgaccca agatacttgc ctgcttgagg
     5401 ccgatagaga ggagaattcc cacttccttg cgacgggcgt tgatccaaag gctgagcaag
     5461 agggcaagga ggagaactga gaagctcaag ctaccccaga agaggaggtt ggccatcttg
     5521 tacataccag agatagattg ctcaagagct gggtagttag aggagctctt gacgagtgtg
     5581 tagctcttcc agttgatacc actgatgcca ttcaactctt tcataacatc atccaagttc
     5641 ttgtctgctg ttacaaagaa ggttgcgtcc ccataaatgg ctgtgtcttc tgtgtatcca
     5701 taaagttttg cagcagtgtg aatgtctgta atagctgtgt tttcgtaaag ttcttgtgag
     5761 taggttactg ctgacttatt atgaccatca aagagtccct tgattgtcac ttcaactgtt
     5821 tccttggctc ctttttcatt atctgcatcg tagatattag agtccagttt aaccttgtcc
     5881 cctactttcc agccgtgttt ggctgccaag tccttgtgca agaggatttt atccttgtcg
     5941 tcgttggtta agtgctctcc ttcgactagt ttataagaac cagagacaaa cttgtcttct
     6001 ttagaggagt cattgacacc tgtaatcatc aagctacttc caaaacgctt ggcacgatca
     6061 gcagtgagat tcttcttggt ttctggcgtt tcaatcaggt catatccagt caaatctccg
     6121 atagcgttga tacgtttgac ataagactcg atggccttgt tttcggtgat ttttttgatg
     6181 tcttcaccct tgatattccc agcaccacga ggcgttcctt ggttgacgcg acgattgatt
     6241 tgcatggaga agctattagt gatattttta aaggtctcct gagaagcctt ggcagtagct
     6301 cccttgattg acaagccgac caaactcaag ctcgccatga ggagaataat caggaagatg
     6361 acaatcgatt tgaaaaactt ccttgtaaca taggcaaatg cgttgtgtaa catagattcc
     6421 ctttctagat tttgttttaa tcattctatc aaaataagct caaattattt actagtattg
     6481 cgcgtttcag tcagtttctt atcctttaat tcaagtgtaa tatctgacgc ttgtgccact
     6541 tctttactgt gagttacgac aatcacacat ttacctgttt tctgggcaag tgatttgagt
     6601 agttcgacaa tatctccagc agttttagga tccagatttc ctgttggctc atcagctaga
     6661 ataactggag cttctgagac caaactgcga gcaatggcaa cacgttgctg ttgaccacct
     6721 gataactgga gaacattccg cttgatctgg ctttcatcca aaccaagctc aagaagtgta
     6781 tccttgcttg cctttttgtt gaccaagcgg atattttcca gcggagaaag ataatctatc
     6841 aagttataat tttgaaagac cagggaaata tggtgcatgc gatggtaaga atagcccttc
     6901 ttacgaatat cctctccttg aaaaaggata gaaccttcaa caggactatc tagaccagca
     6961 agtagggaca agagtgtgga ttttcctgct cctgactccc caataatact gtaaaatttt
     7021 ccaggttcaa aattataatc gatctgatat aggactgctt cagcagtatt cttataacgg
     7081 taggtaacat cttgtaattg taataaagtc atgatttctc cttcttaact aatagatgat
     7141 aaaatttctt tcggtgattt tctaaataag aataggaaac aaagggctac agataagcaa
     7201 ctaagcagaa ctagaaaaac ataggattct gcaaaagata agatgctagt tgataaactg
     7261 cttgctttgg ctagtgtatc ttgtaagctt gcctgatctc cacttgctag tagagtttgg
     7321 agtaggtaac ttgtgattcc gtttcctgca acaaatgctg gaagcaaagc tccaagagat
     7381 accaaaacta cctctaaaca gaattgtagg aagatcgagc tcttgccttt tccaagtgca
     7441 agtaaaatcc ccacttcata gacccgttct ctcaaccaga gagacaaaac cagaattaag
     7501 gctccagctc ctgctatcaa catcccataa aggaagatgg tcaagaaggt ttggaaagtt
     7561 gcaactgagt ctttgatttg ttcaaaagcc ttgttttcct tttcgacttg gtagccttga
     7621 ttttccaagg ccaagttttc tacctgcttc atgagtccgt ccatttcctt aggattttct
     7681 acatagaagc gtgctgcact gacttgagct tcactattgc ccaaaagggt ttggctactt
     7741 tcatagtctg taaagacttg attttcactg aagtcagaag acaagcctgt gaatttctct
     7801 tgttttttac cagaaaagat gccgataatc tcaaactcta ctgtttgtcc ttttccagat
     7861 tcagactgac cagcatccaa gccaatcttg tcatgaagtg aaagaccgtt cttcttagcc
     7921 aattcttcgt ggataaggat tttcttggaa tccccttttt gaaggtgtcg cccttctttt
     7981 agattgaaag ccgaactggt aaaggttaca tccttggatg aatcctcaag agccgttaag
     8041 ctaaccaagt tattgtctgc agctgataaa tcatcacgct ccacgctctg ctcaccagtc
     8101 actgcttcct tgtcttttag ttttgcgacc gtctcaagtt caggagagac attttccagc
     8161 cccttaatct tgcttacaga tgctaggtct gacaacttga atgtctgacc attctctatc
     8221 ttcttaatag aaaaagatgt attgagtgat ttataaagat tgctttctac tgttttgttg
     8281 gacttcatca gagtcaaaca ggctgaaatt ccggccaata agaccaataa aatcagaaat
     8341 aaaataaaac ttctcagtcg ctttctgctg acataagccc aagatctttg gattggattc
     8401 atttgtcacc tccatatttg taagactatt ataaaaccca aatatgaaat gtttatgaaa
     8461 tacgaaaaaa aatatcgagt aggggataat ctctagcccc tctcacacca ccgtacgtgc
     8521 cgttcggcat acggcggttc aactaacttt taacgcatgt cgttcaaggt aataatccaa
     8581 acacgaaacc tgt
//
LOCUS       DAVBGY010000071         8575 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVBGY010000071 DAVBGY010000000
VERSION     DAVBGY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8575
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.071"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(122..604)
                     /gene="greA"
                     /locus_tag="VQ620_001828"
     CDS             complement(122..604)
                     /gene="greA"
                     /locus_tag="VQ620_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET2764407.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(675..2324)
                     /gene="mltG"
                     /locus_tag="VQ620_001829"
     CDS             complement(675..2324)
                     /gene="mltG"
                     /locus_tag="VQ620_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET2764408.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMTDSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDIIS
                     DTSRRSRREGAKPVNPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPIDA
                     NSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQKSM
                     STEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAEAFLA
                     KVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDEML
                     AAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDMPL
                     QSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIESSI
                     NQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(2405..2899)
                     /locus_tag="VQ620_001830"
     CDS             complement(2405..2899)
                     /locus_tag="VQ620_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2764409.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(2896..3360)
                     /locus_tag="VQ620_001831"
     CDS             complement(2896..3360)
                     /locus_tag="VQ620_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2764410.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     DLDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(3370..4704)
                     /locus_tag="VQ620_001832"
     CDS             complement(3370..4704)
                     /locus_tag="VQ620_001832"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET2764411.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(4715..5332)
                     /locus_tag="VQ620_001833"
     CDS             complement(4715..5332)
                     /locus_tag="VQ620_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET2764412.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(5380..8478)
                     /locus_tag="VQ620_001834"
     CDS             complement(5380..8478)
                     /locus_tag="VQ620_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2764413.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDSFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLNRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
BASE COUNT         2338 a         1956 c         1640 g         2641 t
ORIGIN      
        1 aagtcagtaa ccatacatac ggcaaggcga cgctgacgtg gtttgaagag attttcgaag
       61 aatagcaaaa tggaaaaagg agtgagtgaa gcacatcgcc tccccactcc tttttctgtt
      121 tttaggctgt tttttcaacc ttcaagattt ttacatcata gctaccaaca ggcgtttcaa
      181 tggttgctgt gtcacctgtt ttcttgccaa tcaaggcctg cccaattggg ctttcatttg
      241 aaaccttacc tgcaaaggca tccgcaccag ctgaacctac gataatataa acttcttctt
      301 cgtcctcacc aatttcttgg atggtgactg ttttaccaat cgctacttcg tcctgggcaa
      361 ctgcgtcgct attgacgatt tcagcatagc ggatttttgt ttctaagcta gagatttgtc
      421 cttcgacaaa ggcttgttca tccttagctg cttcgtactc actgttttct gaaaggtcac
      481 cgtatgaacg ggcaatctta atgcgttcta ccacttctgg tcgacgaacc aatttcaatt
      541 cttctaattc tttttcaagt ttttcctttt cctcaagggt cataggatat gttttttctg
      601 ccatttttct caactttctt ctgataatat tttctaaaga aaattatgtg acgtatcaca
      661 taattttagt ttgtttagtt taatttgctg ttgacatgtt cagcgacatt gcggtcgtgg
      721 tcttcttgat tgttagcata gtagaccttg ccttctgtga catctgctac aaagtagagg
      781 ttatcgctct tagtttgatt gatgcttgac tcaatcgcat ccagacttgg actatcgact
      841 ggaccaggca tgagacctac atttttataa acattataag gtgaatcaat gttggtatca
      901 atcgcaacat cctcagctag actgatattt tgccccagtt ttccttgggc atacaagatt
      961 gcaatattac tttgaagtgg catatcacga tttaaacgat tgtagaatac acctgcaatg
     1021 agcttacgat cttctgtctt ggcaccttct ttttcgacca aggaagcaat ggtcaacaac
     1081 tcattgacag tcaaattttt agatttgata gtactatagt aaggagatag gttcttatcc
     1141 atagcagcta acatctcatc aatcaagctc tcaatagttg tgctttcctt gatagagtat
     1201 gtagctggga aaaggtatcc ttccaaacga taacgcgcac cgctgtcttt tacaggcaaa
     1261 ctttccagta aagtaggata tttcgctact gcttgactga taaacgtctc atcttgaact
     1321 ttagccaaga aagcctccgc tgtcaaagac tctttgaagt caccttgcaa ttgacccaca
     1381 gcttgagcaa tctgatccaa ggtataacct tctggaattg tcaaagtcgc aagtacaggt
     1441 tcttgcggtt catctgttcc acctttttgc aactctttga gtaagtcttc tgtactcata
     1501 ctcttttgca aattgtagta acctgctttc aagtcggtat aatttttata cttggcataa
     1561 aaactaaaaa tcagaccatg ctttaccaaa ccagcttttt caagcgtcgt accgatttct
     1621 tgaacgtttg aaccttctgg aattccaacc gtcacatatt tcttagaatt agcatcgata
     1681 ggtaacaaag aatctagcac gtactggtaa ccaaagtaac cacctgctga gagcaaggca
     1741 aggaatacaa ggaatgaaat cacaaaagct ttcacatgtg acttcttctc tttcttagga
     1801 ttaacgggtt ttgctccttc tctacggcta cgacgtgatg tatcgctgat gatatccact
     1861 gtttctgtag caggcgtagg tgtttctgga cctgcttgtt ctgctttttt ctctgccgtc
     1921 gtataggaaa cagctaccct tgttggggtt tcattgtatt ctctttcaag tttcttaggt
     1981 ctaacaggac ctggtcttga tccactttct tccgctggag aagaaggtac atcttgactt
     2041 ggatgacttg gaacaccagg agtttctctt tgaatctcat ctgctggaga agctggtaca
     2101 ccttgacttg ggtgagtagg cacggtagga gcttttctca taatctcctc taccgttgac
     2161 aaggaatcag tcatgagttc ttcagctgaa ggttcatttg caggagtgcg aactactgcc
     2221 tcatcttctt tcagaacttc atcatagcct tttacttttt ctaaatctct cagaatctgc
     2281 tctttaaagc ttaatttctc ttcttctctt gacttttcac tcaaaagttt ttcctccttg
     2341 ttgataatcc ataatattat atcttaaaag tccaaaaaaa gcaatctatg atacttttcc
     2401 taacttattt tttcatttcc cagaccatat cataccatgt ttcccctgca aaggttgact
     2461 gggactggcc ttcgttgaca aatccatgtt tttcataata ggcgatgaga tagtcatgac
     2521 aggttagatt aatgccatct ctctcatctt caagagcaac ttctttcaaa gctgtcagca
     2581 atttctgacc cagtccgagt ccctgtgcct ccttggcaat agacagacag gtcacagaga
     2641 tataaccacc aggctcatga ctatagtctt ttatttcttc tgtaaaagac tggtcttgca
     2701 gatggcggtg caggccaact ggtccttcga tataacccat gattcttcct tctttttcag
     2761 caaccagaaa agaggtctga atttctctca aatgtgcttc aaagacagaa ggaggaatgg
     2821 cttcttcgac cgaaaaattt tcaaattcaa gttcaacaat ccgatccaaa tcttctaatc
     2881 ttgcttgtct gattttcatt gttcctccag ataaaaggga ttaaaccaaa tcatactata
     2941 gccctggcta gttgcataga gcaaagttgc ttcttcatca acaaaaccgt tcatttcaaa
     3001 ataggaaagc agctcatcag gactctccaa acgaatccct ttgtaatcca gatcaactgc
     3061 cacctctttc aaggctgcaa gaagaagtgt tcccaagccc tgtctctgat ggtcagactc
     3121 gatgactaaa gaatgtactt ttagatattg cggattgtct gactggggac ttgataaaat
     3181 atagcctaaa agttgatttt catccctagc tagaagaaag gtatccgcac acttacggat
     3241 actttcttct aaaatatggg aaagttgctg cttttcagct ggaaaagacg aggtctgaag
     3301 tgcccctatc tcaggcaaat cagacttgct tgcctgaatg atcttaattg gaatttccat
     3361 gggaacatcc tattgaacat tgcttgtcaa gttagacaag agacgctcaa atgagtattc
     3421 ataggtttgg atgtctcctg ctcccataaa gacgtaaaca gcattgtcat ggtctaggag
     3481 cggagaaaca ttttcaacag taatcacttg gtgttttttg ttgattttgt tggctaggtc
     3541 ttctacctta acgtcaccat gatctacttc acgagccgag ccataaattt gcgctagata
     3601 aacagcatct gcttggttta aagcatgggc aaagtcgtcc aacaaggcaa tggttcttgt
     3661 aaaggtatgc ggttgaaaga ctgctacaat ttccttgctt gggtatttct gacgagccgc
     3721 atccaaggtc gcaataattt ctgttggatg atgggcaaag tcatcgataa tcactgtatc
     3781 attgacaatt ttctcagtga aacgacgttt aacaccggaa aatgttttca agtgctcacg
     3841 caccaagttc aaatcaaatc ctgctgtgta aagaagacca ataacggctg tcgcattcat
     3901 gatattgtga cgaccaaagg ttggaatgtg gaattgcccc aagttttgtc cacggaaatg
     3961 aacggtgaag gttgaaccag ttgttgaacg aagaagatca ctagctacaa agtcattgcc
     4021 ttcagcttca aaaccataat aataaattgg tgcatcagac gtaatcttac gcaattcagc
     4081 atcttcacca tagacaaaaa gacccttggt gatttgtttg gcatagtcgt taaaggcatt
     4141 gaaaacatcc tcgagacttg tgaaataatc tggatggtca aagtcaatgt tggtgataat
     4201 agagtattct gggtggtaag gcatgaagtg acgctcatat tcgtcagatt caaagacaaa
     4261 atatttggca ttggccgaac cacgacctgt cccatctcca atcaagaagc tggtatctgt
     4321 aatgtgagac aagacatgag acaacatacc tgtcgttgaa gtttttccat gtgctcctgc
     4381 tactcccatg ctaacaaagt cacgcataaa gctacctaga aactcatggt aacgtttgta
     4441 gctgatacca ttttggtccg cataggcaat ttcgacgttg ttatctggac gaaaggcatt
     4501 tccagcgata atttccatat caccgtctag attcttttca tcaaaaggaa gaatggtaat
     4561 tcctgcctgc tcaagaccgc gttgggtaaa gtagtacttt tcaacatctg atccctgaac
     4621 cttgtgcccc atctggtgca acatcaaggc caaggcactc atccctgatc ccttaattcc
     4681 gataaaatga tatgtctttg acatgttttc tcccctattc tgtcattctg gtcagattca
     4741 actcttgggc aacccgacgt tcttgttctg tttgtttact ttttttattg tagatttggc
     4801 tcttctttag aaaatcataa ttgtttttct ttggagcagg tgctgacact tcttcattct
     4861 tggtagggat agaatgaact tcttccgcca agatataatg agactgggtc aatttttggc
     4921 tatatttgac aaattcacca ggattttcct tttggaaagg agttgtcggt tgattgccct
     4981 gtctaactag actgggctga gaatgacgtc tcgcaaggct gaaatcctga gttagatagt
     5041 tagcagagcg tttctttttc aagtctgcac gcgcttcttc acgcgccacc tccgcatagc
     5101 tctttccttc ttttttaacc cctaaaggag cttttttagg tttccctact tgcttttcaa
     5161 tcggttttac tggtttttct tcagcaatag gagcccattc taaataattt ttatctcgat
     5221 actcaccctt gatattactg atcagatcag actcatcata gagattcatg actggcattt
     5281 cagtcaacat gacctcgtca tctgacacca atggaaatcg ttcttgtttc attttctatt
     5341 tcctttcaac acttcattat agcgtattgt cttgattttt caagtgctgg cttcagaaat
     5401 tcccaaaatt tctctaattt ctgctagggt cagactacca cgtgactctg tgccgtccaa
     5461 tacttgtgac accagatgtt tcttttgttc ttggagttcc tgaatttttt cttcaatggt
     5521 tcccttggtc accaagcgat agacctcaac cgtttcttcc tgacccatcc gatgggcacg
     5581 gccaatggct tgcgcttcca ccgcaggatt ccaccaaagg tcaaccaaga tcactgtatc
     5641 tgcacctgtc aggttcagac cgaccccacc agccttgagg gaaatcagaa aggcatctct
     5701 ttctccttga ttaaaggcct tggtcatgtc ttgtctttcc ttggctgggg ttgaacccgt
     5761 aattttaaag gaagtcaggc ccaagtctgg cagttcttgt tcaatttttt ccaacattcc
     5821 cttgaactga gagaaaatca agacacggtg tccgccgtct gccacctgta ccagtaggtc
     5881 tcggagacta tctagtttgc cgctggctcc ctgataatct tccataaaca gggcaggagt
     5941 gtcacatatt tgacgcaagc gcatcaaacc agataaaatt tccacacgac ttcgctgaaa
     6001 ttcctgttct gacacttgag ccagatggtc tcgcatctgt tgtaactggg caaggtaaat
     6061 agccttttgc tggtcttcca gttcattttt ataaaccacc tcaatcaagt ctggcaattc
     6121 agtcagaact tcttctttct tgcgtcgcat cacgaaaggc ttgataaact gagccactcg
     6181 ctctgctggc aatttcataa attctttctt gcttggcaaa agtccgggca tgacgatttg
     6241 gaaaatagac cacaactcac ccagatggtt ttcaatcgga gttcctgaca aggcaaagac
     6301 cgacggcacc acaaattgtc tcaaggtctg ggcaatcttg gtctgggcat ttttcatgac
     6361 ctgagcctca tctaagaaaa ggaagtcaaa ggccatcccc tgataaaact cactgtcctg
     6421 acggaaggtg gcatagctag tcacatagat ttgatggctc tcggcaagaa tctcctcacg
     6481 acttgctttc aaaccatgaa caacagccac atccaactgt ggggcaaatt tctgaaactc
     6541 atccgcccag ttgtaaatca aacccgacgg agcgagaatc aagactcgac tttcttttgt
     6601 cacttgacta gtcaaaaaag caatggtctg aagggttttc ccaagtccca tatcatcagc
     6661 caaaatccca ccaaaaccat aatgatggag catctgcaac cagccaattc ccttttcctg
     6721 ataatctcgc aagtcagcct tgacctgagt tgcttgcaaa ggaaagtcct ctggatgcgt
     6781 caaatcctgg gccagattct ggaattcttg tgaaaaagaa acacggtctc gcccttcaaa
     6841 gagatgagct aaactgtagg ccaaggattt ccgagcctgc aaggtcccat cttttaattc
     6901 aaattgcccc agttcctgta gattttggcg aattttcttg gtttcttcat cgaaaaagta
     6961 aacttgatta gacgaatcaa tataaaaatc ctgattggca accaaggcct gcatggcttg
     7021 gtcgatttcc tcctgggcaa tattttgaaa atcaaactgg atttccaaga gacctccctt
     7081 ggaggcaatc tgcacctgag gactcgctag gctataaagc tcttctagtt tatctgatag
     7141 gtcaacatgc ccgagttttt caaagactgg aatgatatca tgaaaaaaat gatagacaga
     7201 ctccgctttt aaggcctgac gccaagattg aaaatcggct tcaaagcccg cagccaaaca
     7261 gacttggaaa attctttctt ctaagtccgc gtcacttgaa aatggtaatt cttctagctc
     7321 ttgtcggcta gatacctgtc tatttccata atcaaactga atttctaaac gaatccgatt
     7381 atcttcttcc ctgtcaaagt aaaaagaggg cgcaaaagtt ttgatttgta gacgttctgg
     7441 agctgaaacg gtgcccatct ggataaaaag agtcagacag gaggccaatt tgtctcgatc
     7501 actgctatca aattgcaggt atttctttcc ttgttgaccc acaggtaacg ctttaatttc
     7561 cttgagaaga cgcatctgct ggtctgttaa aaaataaacc tgacctttat ggaaaagtac
     7621 tgctccctga taaaagacat tgaccctagg actctcactg atttccattt caaaatagtc
     7681 cgagtattct gttactgtaa aggcaaatag attggcatca gcatgcatat cctgaaaaag
     7741 cagggtttgg tagctatcca cttgatggtc aaattgaaaa tggggcaagg ccatcagtaa
     7801 attcacaccc tgctcaaaaa aggtcagagg gaaaaagagg tgccgacctt ggttttggaa
     7861 aaagaggtct ggaaccagcc cttcctccgt tagtccgtgc aagaaagtca aaagttcttg
     7921 gctggcatca tcaaaggctt cccaagaaag agactcctca taaatcttgc caatcatata
     7981 cgactttctc tgctcgacaa tccttaaaaa aagtggaata tcccgaatga catagtattt
     8041 ttggctattg atttggccga ttctcagagt ccacaagata tgattggttc ctgcttccac
     8101 ctgacccaca gctgataact cataggcgca ttctgatttt ggagataaaa ttcgattcaa
     8161 aaacttgcca cccaaggtca ccttggtttc aacggcctct ttttcttcat gaccttcttc
     8221 cagactccac aagatttcct gaccacgctc atcatttttc agaaaatgct ctagcgctgc
     8281 caaatgcaca cagtagcccc tcttttgaaa agaatcacag gcacaaaaaa ccaaatcatc
     8341 ctctaaacta tagcgcagtt cttcttctgc aacgcgagcg tagagccgat tgttcttttc
     8401 cttgatgata tcaaccttac cagtttcata aagggcaaca ccttcgatac gaattttccc
     8461 cggaatcaat ttagccatat tttcaccttt accttatctt tttattatac catattttcg
     8521 cctatgaaaa tagccttcta ggaagacttt tctcctagaa ggctggattt ttaac
//
LOCUS       DAVBGY010000072         8559 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVBGY010000072 DAVBGY010000000
VERSION     DAVBGY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8559
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.072"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            221..2830
                     /locus_tag="VQ620_001835"
     CDS             221..2830
                     /locus_tag="VQ620_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HET2764414.1"
                     /translation="MDYNFNLEHPFFFTNNDYSTDTSIKYQVSLPFNWHEVMNNDEWV
                     YQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICHEMRIPFKHLSTEDKFIMRNGK
                     LVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYILSDKRWDEAPIYLRYGVFR
                     PSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPDFLNKWLDKKDKKQGDFPFI
                     IDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFDGTYSSEKLASECKALKILN
                     EWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFPLYEVVDKTKDYLLRVSKIV
                     EKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWEQAVFSNDEKVVHKVAAPGF
                     RAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVSQTRIEGKKLFESLGYTREH
                     IDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIESEQDIQDFIIKLLRGFTLTYG
                     QWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLAKKVKNFKFDDYYEIRTQVI
                     NEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVELFTTHFIEIFENSQNKNLYSG
                     QAGILLVGLYFLSKGEIDNKLGEEILIRLREYTLNYIENPETFCKVGASDVQSNDPYE
                     NFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVDKELVAYKVDSNNSLQYSQGHR
                     LLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERATDVVFCVLPGLFNGFCGLEVA
                     NNIYSDIDDNFSGQKKLIEQLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAIGLV
                     SIMDNKLTILPQI"
     gene            2863..4803
                     /locus_tag="VQ620_001836"
     CDS             2863..4803
                     /locus_tag="VQ620_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET2764415.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEELFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGLSSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDRVLLFLERRTFYEKILSFDLIENTLKTEFERPIIKSNNTKYFSKVIWTKKDSYD
                     VSIPISLFWKSEDTDELPRRKKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHV
                     RGGGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGA
                     VLNEEKNLLQSILLFSPFINPYDTLQNPNDPLSKTEIAEWGDIRDPEVKAYIKSYSPM
                     QNIEKARDSNTVIVNILGEKDPYINNNEVIEWSKKLNSIGVKSLLYLNKAAGHGGFTP
                     SDVLLMIDTLNYFFEEVGRNNL"
     gene            4800..6407
                     /locus_tag="VQ620_001837"
     CDS             4800..6407
                     /locus_tag="VQ620_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2764416.1"
                     /translation="MNYLKFIKKTKLILMGILIFLSSFNGVLLSGIIVYAGSLNQTSS
                     FSDVLRFGAISILGWSAIYISNYYLEVTEASITKDINVKIKQGYFREQYLSSEMVKDY
                     SSIISVLSNDLRLIEENYFRQIFEIISSILLFIVSLSFMLYLNFLVSIIFIVLSALPI
                     IVPVFMKKMLSNSANEYSNSNAEYTHIIKEIFNGFKTLKSYSVTKEIISLSDKKLDKL
                     EDSTFNLKRSEVLSKLVAVLISGFCFLVPLVVGCYFVIYHKSLSFSELIGIFLANDKV
                     LGPIQSIAYSLNKINTTKDLRKPFLKYLSGEKNFIDAEHDNNGLYTSSIDEIHMKDVV
                     YSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLINGSLKPQKGYVNLLSHGKKS
                     SDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVLKKVNLYEELEKIDILNYQC
                     GENGSNLSGGQKQKIALARALIRNNKVYLFDEISANLDNDNSNSIHDILFNLGISFIE
                     VSHHYDLNDKRYTDIYKLENGTLFKIK"
     gene            6422..7162
                     /locus_tag="VQ620_001838"
     CDS             6422..7162
                     /locus_tag="VQ620_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2764417.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            7165..8484
                     /locus_tag="VQ620_001839"
     CDS             7165..8484
                     /locus_tag="VQ620_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764418.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIASKWNLLYRKPY
                     IKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIIS
                     IAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLTFVTSLIAFLLNIKIMALIYY
                     SLEDVLIDDMNLLGLILPNFIVSIILFILIFITKSSYFIFKNAKIIS"
BASE COUNT         2950 a         1060 c         1601 g         2948 t
ORIGIN      
        1 cgttgcggta actggagtgc ttttagttgg gaaaattgtt aattcattta aattgtaaac
       61 tttagagatt ctctggagtt atggatgtcg ttccaatatg tgcacgttgg aatgttagtg
      121 cttatatggg gagaatccaa aggattctcc ctttttattt aaataagtct gtctagctat
      181 agatatatta agtttgataa aatttcttgg agattttgtt atggattata atttcaattt
      241 ggagcatcct ttctttttca ctaataatga ttatagtaca gatactagca taaagtacca
      301 agtaagtctg ccttttaatt ggcacgaagt aatgaataat gatgagtggg tttatcaata
      361 ccctattgga aaatttgttg aaagacaagg gtggaaaata cacattagtt cagagtataa
      421 cagttcacat gaattgttac aagatgtagc aaaaatttgc catgaaatgc gaattccatt
      481 taaacatctt agtacagaag acaaatttat catgcgaaac gggaaattgg ttagtcgagg
      541 attttctggt aaatttatta catgttatcc aaatcaaaat gaattagagt cggttttgca
      601 aaggttagaa agtgccttaa agcaatataa tgggccatat attttaagcg ataaacgttg
      661 ggatgaagca ccaatatatc tgaggtatgg tgtgtttagg ccgtcaaggg atgatgaaaa
      721 gaaggttgct attgatgaat taattgttgg cgatgaagtt gttaaagatg aacgtttacc
      781 agtatttaaa attccaaaag gtatagtgcc ccctgacttt ttgaataaat ggcttgacaa
      841 gaaagataaa aaacagggag actttccctt tattatagat aatgcaatta gattttcaaa
      901 cagtggtggg atatataatg ctaggctaaa agaagatggg aaaaaaataa ttttaaaaga
      961 ggctagaccg tatacaggtc taggatttga tggtacttat tcttcagaga aattagcttc
     1021 ggagtgcaaa gcactaaaaa ttctaaatga atggagtgag acgcctaaaa tatactggca
     1081 cggtaagatc tgggagcaca ccttcttagg aattgaacat atgaagggcg ttccactaaa
     1141 tagatgggtt accaataatt ttcctttata tgaagttgtt gataagacca aagattatct
     1201 attgagagtt tcaaagatag tagaaaaact catcgattta acgaataagt ttcattctga
     1261 aaatgtatat catcaagatt tacatttagg aaatatcttg gttaaggatg aagatgagat
     1321 tagtataata gactgggaac aggctgtctt ttcaaatgat gaaaaagttg tacataaagt
     1381 tgcagcccct ggttttagag catggcgaga aacacttccc agtgagatag attggtatgg
     1441 tattagacaa atagctcatt atttatacat gccactagta actacatcgg atttgacata
     1501 caactatgta agtcaaacca ggatagaagg aaaaaaacta tttgagtctt tgggttatac
     1561 aagagaacat atagattatg tagaaagttt attgtcatat ctagattcta aatgtccaca
     1621 gattgagaat atttccagga aaaaagtttt aaaacccatg catgagatta gaactatcga
     1681 gagtgagcaa gatatacaag attttattat aaaattattg agagggttta cgctaactta
     1741 tggacagtgg cgtaaagaat ttcaatcaag attcttccca gttcattatt atggtctgaa
     1801 ttttaatcaa ggaattgcgt tttcagattt agctatcctt tggagttatc aacaactagc
     1861 taagaaagta aaaaatttca agtttgatga ctattatgag attcgaacac aggttattaa
     1921 tgaggcagta aataatttta agaaatcgtc cctttctggt ctttttgatg gtaaaattgg
     1981 tacaatctgg ctcatttatg aatttgggga gatagatcgg gcagttgaac tttttacaac
     2041 tcattttata gagatatttg agaattcaca aaataaaaat ttatatagtg ggcaggctgg
     2101 aattttactg gttggactgt attttcttag taaaggggaa attgataata aactaggaga
     2161 agaaatacta attaggctta gagaatatac tttaaattat atagaaaatc cagaaacttt
     2221 ttgtaaagtt ggcgctagtg atgttcagag taatgatcct tatgagaatt ttggtggatt
     2281 actatatggt cacgcagggg tagcttggct cttcggtgaa gcttataaat taactggtga
     2341 aagtatctat aaaaatggtc tagaattggc tgtagataaa gaattagttg cttataaagt
     2401 agatagtaat aattcattgc agtacagcca aggtcataga ttactaccat atctagcaac
     2461 tggaagtgct ggcctgcttt tacttattaa tagaaataaa gaaatcttgt caagcaaata
     2521 cttaaaatat ttaacttctt tagagagggc aactgatgta gttttttgtg tattaccagg
     2581 tttatttaac ggattttgtg gtcttgaagt tgctaataat atatatagtg atatcgatga
     2641 taacttttca ggacaaaaga aattaattga acaactttat agatatttgt gtgtaattga
     2701 agaaggattt gttattgcgg gagataatgg tttaaagata acaacagata ttgcttcggg
     2761 atttgcgggt gttgcaattg gattggtatc aataatggat aataaattga cgattcttcc
     2821 tcaaatataa tatttagata ccattattaa ggggtcatac ttgtgaatca gagtaatgaa
     2881 atatttttaa atacaataca ggaaaaaaca cactataaga ggtctatctc ttcatcattt
     2941 caggaattaa aaaaatggaa ttctttgata tttaggagag ggcgatattg gtaccaattt
     3001 gataattgga gtatacccaa tactggtgta ataaaggaat ggtatgtcga agataatgac
     3061 tggattccta aggttagttc ttccagttta gacaattcaa attcacttaa aattgtcttt
     3121 gatgtggaaa aatatacttt gccagatgat agtatcttac aggatgttag gatttcatct
     3181 gatgagagtt atatagtttt ggttgtatcc tcactgcgta gcactaattt aattggaata
     3241 aaaaaatata ctatggaaga gttatttatc attgaagata tttcagtaga aagttcgttc
     3301 tatttgggta aattcggtgt tatgtatact cgctccaaag agtacgggag gccaagtaag
     3361 ttattctata aatcttttga tagctttaca gaagaggaac tgtttgaaga aaacgaatgc
     3421 tcttttcgat taaaaatagt ccacattgat agcaataatt gttttgtaaa gtcagtagac
     3481 tttcagaaag gcaggatttt tctatacagt ttcgaccgga cgggttttgt tagacactct
     3541 tacacagaaa cggtagcccc aacacctaga gatatcgctc tatttagtac agaaaaagca
     3601 cagtactttc ttgggctctc ttcaactgaa gaaaagaaag atcaactaat tctaagggaa
     3661 acttcttcgg gagacagagt ttctattact ataccatatc aagatagggc tagaagagtt
     3721 cactgtatag gacgttatat ccttttagac tgttcaaacg cagcgaattc cgtcttttat
     3781 cttgctacat ttaaaaataa tagtttgagg gagttgagtg ttaataagat aacatttgat
     3841 gaaaagacaa cactttatga aaattcattt tctgatcgtg ttcttctgtt tttagagagg
     3901 agaacttttt acgaaaaaat acttagtttt gatttaatag aaaatacatt aaaaactgaa
     3961 tttgaaagac ccatcataaa atcgaataat actaaatatt tttctaaggt gatttggact
     4021 aaaaaagaca gttatgatgt aagcataccg atttctttat tttggaaaag tgaagatacg
     4081 gatgagctcc caagaagaaa aaaatgtatt ctaagtgtct atggggctta tggtaagaat
     4141 gataattctg atttagatga aattatgtta tctataatag atgcaggttt tatttatgct
     4201 atagttcatg taagaggtgg aggctatttg ggtggtgaat ggtatcgctc aggaaaggcg
     4261 ttaaacaaat ggaattctat cagagatttt attgagggag taaattattt aagggaaaat
     4321 gatgtaattg acagtaagcg attaggttta ataacttcta gtgcaggtgg aataattgct
     4381 ggtgcagtgt tgaacgagga aaaaaattta ttgcaaagca ttctcttatt ttcgccattt
     4441 ataaatcctt atgatacact tcagaatcca aatgatcctc tttctaagac cgaaatagca
     4501 gagtggggag atattagaga ccctgaagta aaggcttata taaagtcata ttcgcctatg
     4561 caaaatattg aaaaggcacg agactcaaat actgttatag ttaatatttt gggtgagaaa
     4621 gacccatata ttaataataa cgaagtgata gagtggtcaa agaaattaaa ttctattgga
     4681 gttaaaagtt tgttgtattt aaacaaagca gctggtcatg gaggttttac tccatcagat
     4741 gttctcttaa tgattgatac tttaaattat ttctttgaag aagtgggaag gaataactta
     4801 tgaattattt aaaatttata aaaaagacta aacttatatt aatggggatt ttaatatttc
     4861 tatcttcgtt taatggtgtc ttgctctcag gaattattgt atatgcaggt agtttaaatc
     4921 agacttcctc attttctgac gttctaagat ttggtgctat aagtattctg ggttggtcag
     4981 caatctatat atcaaattac tatttagaag taacggaagc atcaataacg aaagatataa
     5041 atgtaaaaat taaacaagga tattttagag aacagtatct ttcttctgaa atggttaaag
     5101 attactcatc tattatttca gttttgtcaa atgatttgag attaatcgaa gaaaactatt
     5161 ttagacaaat ttttgaaata atttcttcaa tattgttgtt catcgtctct ctaagtttta
     5221 tgctttattt aaacttccta gtttcaataa tatttattgt attatcagca ttgccgatta
     5281 tagtacctgt ctttatgaag aagatgttgt ctaattcagc taatgagtac tctaacagca
     5341 atgcagagta tactcacata ataaaagaaa tttttaatgg ttttaagaca ttaaaatctt
     5401 attctgttac taaagaaata attagtttgt cggataaaaa gttggataaa ctagaagatt
     5461 ctacttttaa tttgaaacga tcagaggttc tttcaaaatt ggttgcagta ctaatttcag
     5521 gtttttgctt tctagttccc ttggttgtag gatgttattt tgtaatttat cataaaagcc
     5581 tttcttttag tgaactgata ggtattttct tagcaaacga taaagtttta gggccgatac
     5641 aatcaattgc ctattcatta aataagataa atacaaccaa agatttaagg aaaccgtttt
     5701 taaaatactt aagtggagag aagaatttta tagacgctga acatgataat aacggactgt
     5761 atacttcatc aatagatgag atacacatga aagatgttgt atattctatt acaccagaaa
     5821 ataaattaag tattgacttc tcatttaagt caccatttag ggtattatta acaggaactt
     5881 ctggtagtgg gaaaacaacg attttaaatt taattaatgg ttctttaaag ccacaaaaag
     5941 gttatgtaaa tttgttatca catgggaaaa agagttcaga ttcaatacca acagttgatc
     6001 agacaccata tatttttgac actactattc gtgagaacgt aactttattt caaaatgaat
     6061 atttttcaga tgatcagata attgaggtgt taaaaaaggt aaatctatat gaagaattag
     6121 aaaagataga tatactaaat tatcaatgtg gtgaaaatgg tagtaatttg tctggaggtc
     6181 aaaaacaaaa aatagcttta gctagagctc tgattagaaa taataaagtg tacttatttg
     6241 acgaaatatc agctaattta gataatgata attcaaattc catacatgat attctgttca
     6301 atttaggtat ttcatttatt gaagtttcac atcattatga cttaaatgac aagagataca
     6361 ctgatatata taaattggaa aatggaacac ttttcaaaat taagtgattg gaggcaagaa
     6421 tatggataaa gattatatat taaaagtgaa agggctgtat catcaatttt tactaggaaa
     6481 taataaaacg ttgcaagtgc tgaaaaatgt ttctctttct gcttcgagag gagaatttat
     6541 aagtattcta ggaattagtg gttctggaaa gtcaacttta ttaaaatgta tttctagttt
     6601 gcttgaacca acaagtgggg aagtaatttt aaatggaatc aacccctata aaatcagaaa
     6661 tgcaaaattg tcaagtataa gacgtaacga agtatctttt atatttcaag catacaattt
     6721 aataccttcc ctgccggtaa tagaaaatat agcacttcct ttgcgattat cacaaaaaaa
     6781 attaactatt aaaaatgtag aaaacttact caaaagaatg aagtttaatg ctggcttaaa
     6841 cgattttgtt ggaactctgt ctggtggaga acaacagaag gttgctatag ctagagcggt
     6901 tattgctgat agtgatataa tatttgctga tgagccaact ggggctttag acagcgtttc
     6961 tcgtgaagta atttttgaat tattgagaga gttagtaggg gcgggtaagt gtgtaattat
     7021 ggtaacgcac gatatagaat tggcctcgaa aactgatcgt gcattaatat tgaaagatgg
     7081 aaaaattttc aaagaacttc atagacctag cggggaagag ttgtataaaa tcttagaggt
     7141 acaatcaact acggaggaat agagatgctc agattagttt actatcaatt tttacataat
     7201 aagaaacaat ggttaggcgt ttcaccagta atttttgttt cgagtttagt tatgggattg
     7261 gctgttaatg gggtaattaa tgttgagaat aattcgcaag tatttgtcgg acttccagat
     7321 cctaaaccaa tttttatgtt tccgatagta ttcggaggag taacattatt ctttgtgctc
     7381 tctaacataa taaatatgtt ggttgaaatt tttagagatg attacgaact tctggaggtg
     7441 ttgggagcaa gtctccttca actatcattt cttgtggggg gacagatttt tatcatttcc
     7501 tcgattatta gttttattgc atatctatgc tctatatttg ttacgtcaaa ttattattat
     7561 tttttacaat actttttcgg ggaaaatata ttacctgata tccaatttca aacgagtgct
     7621 gttggatgta tcattacagt agtgttgatt tcatttttag cctttctaag tggttgcttt
     7681 tatacgttca agaagattag aaataggaag agttctaaaa taaggcatgt tctttcaata
     7741 gtaaaaagaa tactgctatt ggctggtttt agtgttatct ggttgctttc tttgcaacaa
     7801 attttccaag atagcacaat tttagcgaag gcccagataa tattcaatat tgtaatctta
     7861 gatattgtga ttatttatca attatctcct tttatacagt catgttttat caaactactt
     7921 tctataatta tatttagaaa taattttatg ttcattgctt cgaagtggaa tttactatat
     7981 cgtaagcctt atataaaatc tatcagtgct gcaattacag gagctatatt actaatttct
     8041 agttttcaaa tgatttctca gaatatacta tctcaatttc aagatgacag tgatttagag
     8101 ctaaaagtgg catttattgt ctatgtagga gcaccaattt tgatagtact tgcaaatatt
     8161 atttcaattg catttttgtc atcgcatcaa gaaaggattg agattcagca atttgaaatt
     8221 ttaggaactt ctaattacca gatggttaag attaaagttg gtgaggctat atttttgaca
     8281 tttgttactt cattaattgc atttttactt aacattaaaa ttatggcttt aatatactac
     8341 tctttagagg atgttttaat agatgatatg aatcttttgg ggttaatact tccaaatttt
     8401 atagtgtcta ttattttatt tattttaata tttattacta aaagttccta ttttatcttt
     8461 aagaatgcaa aaattatttc ttaaaaattt gtaagttttc gctatataaa atagttaaat
     8521 ctattttgta tcattgcttg cattttactg gtggagaga
//
LOCUS       DAVBGY010000073         8472 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVBGY010000073 DAVBGY010000000
VERSION     DAVBGY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.073"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(162..365)
                     /locus_tag="VQ620_001840"
     CDS             complement(162..365)
                     /locus_tag="VQ620_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2764419.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(358..1440)
                     /locus_tag="VQ620_001841"
     CDS             complement(358..1440)
                     /locus_tag="VQ620_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET2764420.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1566..2348)
                     /locus_tag="VQ620_001842"
     CDS             complement(1566..2348)
                     /locus_tag="VQ620_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET2764421.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2348..3691)
                     /locus_tag="VQ620_001843"
     CDS             complement(2348..3691)
                     /locus_tag="VQ620_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET2764422.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(3790..5106)
                     /locus_tag="VQ620_001844"
     CDS             complement(3790..5106)
                     /locus_tag="VQ620_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HET2764423.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5299..6525
                     /locus_tag="VQ620_001845"
     CDS             5299..6525
                     /locus_tag="VQ620_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HET2764424.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGSGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIVLALS
                     YYLGRSIKKESQKPLT"
     gene            6724..8298
                     /locus_tag="VQ620_001846"
     CDS             6724..8298
                     /locus_tag="VQ620_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2764425.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
BASE COUNT         2421 a         1638 c         1878 g         2535 t
ORIGIN      
        1 cctgtgcaat acagaactaa atccttcgga taaattaatt gtctaactgt ttgggggcag
       61 tacatattca acacctgata ctatgcgttt tattgtggga agattttcct cattttctag
      121 tgaaattgaa ctttttctaa catcttttga tagatgactt cttaatcaat tatcatttcc
      181 attgttgttt cttggggttt catcccttgt ctttgataaa agcggagagc accctcgtta
      241 tcattccaga cacttagggt tagattattg cagccaattt ctttagcata atagcagaaa
      301 aatgacaata aaatagcccc tagatatttt gaaaggaaag cataggggct atatagttta
      361 atcaaaataa agcaaatctt tgctggtgct tgtaaagagg tcgaagccat ccttggtaac
      421 aacaccgcag tcttcaatac gaacaccgac tttaccaggg atatagatac ctggttcaac
      481 agagaagcac atgccttctt cgatgaccat gtcgtttcct tccatgatag atgggaattc
      541 atggacatcc ataccgatac catgcccgag acggtggttg aagtactcac cataaccagc
      601 tttttcgatg acctcacggg cagcgcggtc cacttcatga gcagtcacac ctggcttgat
      661 aaagtcaaga gcagcttgtt gggcttcaag agtcaagttg taaatatctt tcttgaattg
      721 gtctggtttg ccgacagcga ctgtacgagt catatctgac gcatagccat tgaccagaac
      781 acccaggtca aagaggagaa gagcatcatt ttcaacctta ttagctgctg gaatgccgtg
      841 tggattcgca gcattatcac cagtcaagac catggtatca aagctcattt cataaccttc
      901 acgtttcatg gcaaagtcga tttgtgcgat gatatctgtc tcagtcttat caagagaaat
      961 attgtcaaaa ccaacatgaa cagccttgtc agcataaaga cctgcaacca tcattttttg
     1021 cacttcatca gctgatttga tgaggcgcat gcgttggata cgaggagtga ggttgtcaaa
     1081 ctcagcagtc tcaaaaactg ttttcaaacc atggtatttg gtcaagatga gattgtcaaa
     1141 ctcaacagcg acacgtttga agtcaagttg tggaagagca tgtttgattt tttgccatgg
     1201 attttcagaa tcgacatagc ccactactgg gaaggaaacg gtgctacttg cacgttctac
     1261 ttcaagagct gggacaaaga ggagaggttc ctgatccgct aggacaaaga ggaacatttg
     1321 gcgttcatgg ggatcactgt aaaaaccagt gaggtaattg attgtgacgg ggtcagatac
     1381 gacagcgacg tctagttttt ctgattcaag atatgttagg atttgttgta atttagacat
     1441 atgctacctt ctttctaccc ctctattttg gcaaaaaatg agagaaaatg caagggagaa
     1501 gggtaaaaga aaagacaaaa aactccgaca agataacgga gttctttgat atcattttcc
     1561 tttgtttaag aaaagtcagc cttgcttttg acgtcactgt actcttcagc gatttcttgg
     1621 ctgagaatgt cctcataggc agggagttgg atgtcctgac catatttctt cttgagggca
     1681 gtagtaacta ggcgataagc cagattggca ttacgagaga ggataggccc gtggaagtag
     1741 gaaccaaaga cattcttata atgaacccct tcaccgacct tttcttcgtt gtttccattt
     1801 ccatagacaa cctgtcccag cggtttttgg tcatcagaga ggaaggtacg accttggtga
     1861 ttttcaaatc catagtaggt ttcatcgaaa tcttcattgt gaatcttgat gtcaccgata
     1921 aaacggttat tggtctggtt gagcgtgtag tgtcccatga cccctagccc ttcgatacgt
     1981 tttcctgaag cttcaacata atattgaccc aatagttgga aaccaccgca gatagccaga
     2041 actacaccgt cgttttggat gtagttgtca atgctctctt ttttagcagg taggtcgtct
     2101 gcaatgatac tttgttcaaa gtcttgacca ccaccgaaaa aggcgatgtc gtagtgattt
     2161 tcatcaaagt catcatggag agaaacgatg tcaacggtca catgggctcc cagtttttca
     2221 gccacatact tgagcatgag gatgtttcca ttgtccccgt aggtattcat gagatttccg
     2281 tagaggtggg caatgttgag ctgatagggg taattgctat cttttgagga aagtgaagta
     2341 taaaccatta gttcatctcc tttctaacaa tctgacgact agccagcagt tcacgaaatt
     2401 ccagcatggc agtataagtt gccagaatat aggcatgctt gcagtcttga ttctcaatgg
     2461 tcttgagaac ttgctccaga ttactcgttt cagtgatttt ctcagctgga tagccagtca
     2521 ctcggaggcg acgagcgatt tcagaatgac gaacaccgcc agcgttgatt tcaggaatgt
     2581 ccatgtcagt gatttgctca aagtctgcat cccagatcca gctagtgtca attccatctg
     2641 catagttggc attaaggagg acagataggc taaatggata aggtgctagt ttgatcattt
     2701 cgatagcttg ggttgcaccg actggatttt taatcaagac aagggtacat tccttgtcac
     2761 cgatatgaaa ggtttcttgg cgtccaaaga cagcacggct cttgtcaaat ccctgtttga
     2821 tgagttggga atctgcgccg aggaaacggg cgatggccac agcagctagg gcgttataga
     2881 tattatagag cccgccgatt tggataccgt attcttggcc gtctatgaca aagcgagagc
     2941 gattgttggt caactcaacc agttttgtca aacgatagtc gagatcagga cgtttacatc
     3001 cacaaccttc acagatatag gcacccaagt ttgcataggt attatgctca tatttgagga
     3061 tgccttggca gtcaggacag agaatccctt cggtattgta gtgagccagt tgggctggtc
     3121 ctttttccaa gtcaaaacca aaatactcta tagggtttgg aatagttggc ttgtagaaaa
     3181 gtggactgtc tccgttaagg agaacagtag cagttggaac ttttcgaatg gcatccaata
     3241 tcatgttata ggtagtatag atttcaccga aacggtccat ctggtcacgg aagatattag
     3301 taatgacaaa aagactaggc tggatatagt cacagatacg agatagactg gcttcgtcaa
     3361 tttcgaggac ggcaatattt ttcccagttt ttgaagattt ggctgttagg aaggttgttg
     3421 caatccctgt aatcatgttg gcaccgcttg ggttggttag aacttgacca taaacctctt
     3481 ttaaaatgcc gacagtgagg gcagttgtca gggtttttcc atttgttcca gtgacaacga
     3541 caatctcgta gttcttagct aggctttgta aaatatcttt atcaaattga agggcgactt
     3601 tccctgggag cgtacttcca cgtccaagac ggcttaaaac gaagtgggaa gaacgcccag
     3661 caagaaggcc caaagtagtt tttaagttca tgtttctatt atatcacaaa agagggaaaa
     3721 gacagatttg gtgtttcgta gaagtaaaaa cagcccaaag agggctgtca gttaagatga
     3781 gatttcaact taaatcgcaa acaagtcatt caagttctca gccaaggttg ggtgagtgaa
     3841 gatttgtttt gtgaagtaag tgtaaggaat cttgttgtcc atagcaacag taatgatgtt
     3901 gatgatttct tgagaacctt ctgagaagat gcttgcacca agaatttctt ttgtttcagt
     3961 attaacaaca gctttgaaag ctccgcgaag gtctccattt acgtgaccac gaggcatggc
     4021 tgcaacaggg atttctttca ctgcgtatgg aagtttcaaa tcagctgctt ggctttcagt
     4081 caaaccaact tgtgaaagtg caggtgtgat gaacatagta tttggcacat tgagacggtc
     4141 ctcaagtgtg tagctgccat ctccagcaag gtagctgtaa acaacacgga agtcatcaag
     4201 tgaaatgtaa gtaaattgaa ggccaccgtt gacatctcca actgcaaaga caccaggaac
     4261 gtttgtttga cagtgtttgt ctactttaat agcaccacgt tcagttagtt caatatctgt
     4321 attttcaagt tgaagtggtt ctacatttgg tttgcgtcca gttgcgtaga gaagggcgtc
     4381 gaaacggtaa gtttcgtctt cagttacgac aagcacttgg tcaccatcgt ttttgatttc
     4441 agtagtatgg atattttgaa gcaattcaat gccatcttct tccatgtatt gtttagcaag
     4501 agctgcgatg gaaggttctg cacgaggtag gaatgtatcc aaggcatcta ggactgtgac
     4561 cttgcttcca agtttgttgt aaaggccggc aaattcaaga ccgatatttc cgccaccaag
     4621 gattccaagt ttttcaggta atttgtccaa gctttggata cctgttgagt caaagatgtt
     4681 tttgcttgta gcaagtccag gaattggcaa gacgtttgaa acagcaccag tgttgatgac
     4741 gattgtttca gcagtcagtt ctttcttttc atcaccagct tggatttcga tgactttatt
     4801 tgaaaggaag tgagcttccg catcaaagat atctacgcct gtaccagcaa cagtcgcata
     4861 gtttttaccg ttgaggcgac cagtgatcgt gtttttagta gcaatcactt cttcaaaaga
     4921 caagtccttt tcagcagcaa ctagcaaggt tttagttggg atacaaccaa tgttgataca
     4981 agttccaccg tacatagcct tgctgcgttc aacgagggca acttttttgc cagctgaagc
     5041 caatttacct gctagtgttt taccagcttt accaaatcca ataacaatta aatcgtatgt
     5101 taacatttat cttcctccta ttcgaatttc attctagcac aagaaaaaaa cttttgcaca
     5161 attctgctac ggaaaagaca gaaaaagata aaaacgcttt ccagacaaaa aactagtaat
     5221 tttttgacaa gcttgataaa ctaaaatcct atctattttt atataagaaa gaataaatgt
     5281 tcgaaaatag gaggttttat gaaatcgaat cgttatatta ttgcctttgc tggggtcatt
     5341 ttacacttaa tgctgggttc gacttatgct tggagtgttt atcgtaaccc tattattgaa
     5401 aaaacgggat gggatcaggc ttctgttgcc ttcgccttta gtctagcaat cttttgtttg
     5461 ggcttatcgg ctgcatttat ggggcgtttg gtagaaaaat ttggtccgaa agtcatgggg
     5521 agtctatctg cttttctata cgcaggtgga aatatcttaa caggatttgc aatagaccgt
     5581 caggaactgt ggttgttgta tctagcttat ggcattttag gtgggcttgg tttgggatca
     5641 ggctatatta cccctgtgtc aacgattata aaatggtttc ctgataaacg tggtctcgca
     5701 acaggtttag cgattatggg gtttggtttt gcttctttat tgactagtcc catagcgcaa
     5761 cacctcatcg caggggtagg gattgtagaa actttttata ttttaggagc aagttacttt
     5821 attatcatgc tcctagcttc acaattcatt aagcgtccaa atgagcaaga gcttgcaatt
     5881 ttatcttctt cagggaaaga aaaaacagcc tctttgacgc aaggaatggc tgcaaatcag
     5941 gctctaaaaa gcaatcggtt ttatatgctt tggattattt tctttatcaa catagcttgt
     6001 ggtttaggct taatttcagc ggcatcgcca atggcacagg agatggctgg cttgtctaca
     6061 agtcatgcag cagtaatggt gggtgttttg gggattttca atggatttgg tcgcttgctc
     6121 tgggcgagtt tgtctgacta tatcggtcgc cctctaacct ttagtatatt actgcttgtc
     6181 aatcttttct tttctctctc actttggctc tttacagatt ccgttttatt tgtagttgct
     6241 atgtctattt tgatgacttg ctatggagct ggtttttctt tgattccagc ttatctcagt
     6301 gatatttttg gaaccaagga attggccgct ctgcatggtt atattttaac agcttgggca
     6361 atggctggtt tagcgggacc tattttatta gcagagactt ataaaatggc tcattcgtac
     6421 acacaaacct tgttcgtttt tctcatttta tacagtatcg tcttggcttt gtcttattat
     6481 ctaggtcgtt caatcaaaaa agaaagtcaa aaaccgctta catgattttc tgtagaaatg
     6541 gggctatctt ttgctctggt cttgtgttat actagatagg ttgcaaagaa aacagtactt
     6601 ttcttttgtg gaaaagaagc aacacgattt tatatctagt tatatgaaaa tacgtcataa
     6661 aaagaaaagt ataaactaag ccctataggg tggcttcgca ccacctttag aaagaagaat
     6721 aacgtgaaat ttaatgaatt aaacttgtct gctgatttgc tagcagaaat tgaaaaagct
     6781 ggttttgtag aagctagtcc tatccaagaa caaactattc ctttggccct tgaaggcaag
     6841 gatgttatcg gtcaagctca gactggtaca ggaaaaactg cagcctttgg cttgcctacc
     6901 cttgaaaaaa tccgtacaga agaagcgact atccaagctt tggtcatcgc tccaactcgt
     6961 gaactagctg tccaaagtca agaagaactc ttccgctttg gtcgtagtaa gggagtcaaa
     7021 gtccgttcag tatatggcgg atcaagcatt gaaaaacaaa ttaaggctct taaatctggt
     7081 gcccatatcg tggtgggaac tccaggtcgc ctcttggact tgattaaacg caaggccttg
     7141 aaattacaag acattgaaac ccttatcctt gacgaagcgg atgaaatgct taacatgggc
     7201 ttccttgaag acatcgaagc cattatttcc cgtgtacctg agaaccgtca aactttgctt
     7261 ttctcagcaa ctatgccaga tgccatcaaa cgtatcggtg ttcagtttat gaaagcccct
     7321 gaacatgtca agattgcggc taaggaattg acaacagaat tggttgacca gtactatatc
     7381 cgtgttaagg aacaagaaaa atttgacacc atgactcgtc tcatggatgt ggcacaacca
     7441 gaactcgcta ttgtatttgg tcgtaccaaa cgccgtgtgg atgaattgac tcgtggtttg
     7501 aaaattcgtg gcttccgtgc agaaggaatt catggcgacc tagaccaaaa caaacgtctt
     7561 cgtgtccttc gtgactttaa aaatggcaat cttgatgttt tggttgcgac agacgttgca
     7621 gcgcgtggtt tggatatttc aggtgtgacc catgtctaca actacgatat tccacaagat
     7681 cctgagagtt atgttcaccg tatcggtcgt acaggtcgtg ctggtaagtc aggtcaatct
     7741 attacttttg ttgctccaaa cgaaatgggt taccttcaaa tcattgaaaa cttgactaag
     7801 aaacgcatga aaggtctcaa acctgcaagt gtagaagaat ccttccaatc aaaaaaacag
     7861 gtagctctca agaaaatcga acgtgatttt gcagatgaaa ccattcgtgc caactttgag
     7921 aaatttggta aggatgctcg caaattggct gctgagttta ctccagaaga attggcaatg
     7981 tatatcttga gtctgacagt ccaagaccca gatagccttc cagaagtgga gattgcacgt
     8041 gaaaaaccac taccgtttaa accatcaggt aatggtttcg gtggtaaagc taagggaggt
     8101 cgtggaggcc gtcgtgggga cgaccgtcga gagcgtgatc gccgtggcaa tggtcgccgt
     8161 gatgagttca aaaaaggaag tcgtggcaac gatcgttttg ataaggaaaa acgttaccgt
     8221 aaggataata aaaaaccacg caatacttta agcgaaaagc aaacaggctt tgttattcgt
     8281 aacaaaggcg ataaataaga aaaagcggtt caaaatgaat cgctttttta tataaggaga
     8341 ccgaatgtga aaagacaaat aaaattacta tattatcttt tataatgtta tgatacacaa
     8401 aaataggcaa cttgtcaata gaaaaggaac aatttaatta aaaatattct ggtttttaat
     8461 taaattgtaa gt
//
LOCUS       DAVBGY010000074         7549 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVBGY010000074 DAVBGY010000000
VERSION     DAVBGY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.074"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            172..1563
                     /locus_tag="VQ620_001847"
     CDS             172..1563
                     /locus_tag="VQ620_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET2764426.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIDQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            1677..2519
                     /locus_tag="VQ620_001848"
     CDS             1677..2519
                     /locus_tag="VQ620_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET2764427.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            2792..3553
                     /locus_tag="VQ620_001849"
     CDS             2792..3553
                     /locus_tag="VQ620_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2764428.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            3923..4840
                     /gene="glyQ"
                     /locus_tag="VQ620_001850"
     CDS             3923..4840
                     /gene="glyQ"
                     /locus_tag="VQ620_001850"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET2764429.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            5100..7136
                     /locus_tag="VQ620_001851"
     CDS             5100..7136
                     /locus_tag="VQ620_001851"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET2764430.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPVVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSELFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            7178..7435
                     /locus_tag="VQ620_001852"
     CDS             7178..7435
                     /locus_tag="VQ620_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HET2764431.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
BASE COUNT         2364 a         1324 c         1689 g         2172 t
ORIGIN      
        1 aggtctacta ttctagcttc aatctactag aaattccata gatagaaaac tacataatct
       61 ctacagatac ggatgttgga gttgatgtaa gatgctttgg cttgctagag gaattgtgga
      121 ttgccaaatt gtatcattga aattattgct caaatttgtt atgatataaa tatgaataaa
      181 agtagactag gacgtggcag acacgggaaa acgagacatg tattattggc tttgattggt
      241 attttagcaa tttctatttg cctattaggc ggatttattg cttttaagat ctaccagcaa
      301 aaaagttttg agcaaaagat tgaatcgctc aaaaaagaga aagatgatca attgagtgag
      361 ggaaatcaga aggagcattt tcgtcagggg caagccgaag tgattgccta ttatcctctc
      421 caaggggaga aagtgatttc ctctgttagg gagctgataa atcaagatgt taaggacaag
      481 ctagaaagta aggacaatct tgttttctac tatacagagc aagaagagtc aggtttaaag
      541 ggagtcgtta atcgtaatgt gaccaaacaa atctatgatt tagttgcttt taagattgaa
      601 gagactgaaa agaccagtct aggaaaggtt cacttaacag aagatgggca accttttaca
      661 cttgaccaac tgttttcaga tgctagtaag gctaaggaac agctgataaa agagttgacc
      721 tccttcatag aggataaaaa aatagatcaa gaccagagtg agcagattgt aaaaaacttc
      781 tctgaccaag acttgtctgc atggaatttt gattacaagg atagtcagat tatcctttat
      841 ccaagtcctg tggttgaaaa tttagaagag atagccttgc cagtatctgc tttctttgat
      901 gttatccaat cttcgtactt actcgaaaaa gatgcggcct tgtaccaatc ttactttgat
      961 aagaaacatc aaaaagttgt cgctctaacc tttgatgatg gtccaaatcc agcaacgacc
     1021 ccgcaggtat tagagaccct agctaaatat gatattaaag cgactttctt tgtgcttggg
     1081 aaaaatgttt ctgggaatga ggacttggtg aagaggataa aatctgaagg tcatgttgtt
     1141 ggaaaccata gttggagcca tccgattctc tcgcaactct ctcttgatga agctaaaaag
     1201 cagattactg atactgagga tgtgctaact aaagtgctgg gttctagttc taaactcatg
     1261 cgtccacctt atggtgctat tacagatgat attcgcaata gcttggattt gagctttatc
     1321 atgtgggatg tggatagtct ggactggaag agtaaaaatg aagcatctat tttgacagaa
     1381 attcagcatc aagtagctaa cggctctatc gttttgatgc atgatattca cagtccgaca
     1441 gtcaatgcct tgccaagggt cattgagtat ttgaaaaatc aaggttatac ctttgtgacc
     1501 ataccagaga tgctcaatac tcgcctaaaa gctcatgagc tgtactatag tcgtgatgaa
     1561 taagcaagaa aaaataggtc tgttagatat ttgacagact tattttttac agaatatagt
     1621 actacttaaa aaatgtttta tgctataatt gatgaataaa atagaaggag aagcatatga
     1681 atacctatca attaaataat ggagtagaaa ttccagtatt gggatttgga acttttaagg
     1741 ctaaggatgg agaagaagcc tatcgtgcag tgttagaagc cttgaaggct ggttatcgtc
     1801 atattgatac ggcggcgatt tatcagaatg aagaaagtgt tggtcaagca atcaaagata
     1861 gcggagttcc acgtgaagaa atgttcgtaa ctaccaagct ttggaatagt cagcaaacct
     1921 atgagcaaac tcgtcaagct ttggaaaaat ctatagaaaa actgggcttg gattatttgg
     1981 atttgtattt gattcattgg ccgaacccaa aaccgctcag agaaaatgac gcatggaaaa
     2041 ctcgcaatgc ggaagtttgg agagcgatgg aagacctcta tcaagaaggg aaaatccgtg
     2101 ctatcggcgt tagcaatttt cttccccatc atttggatgc cttgcttgaa actgcaacta
     2161 tcgttcctgc ggtcaatcaa gttcgcttgg cgccaggtgt gtatcaagaa caagtcgtag
     2221 cttactgtcg tgaaaaggga attttattgg aagcttgggg gccttttggt cagggagaac
     2281 tatttgatag caagcaagtg caagaaatcg cagcaaatca cggaaaatcg attgctcaga
     2341 tagccttggc ctggagcttg gcagaaggat ttttaccact tccaaaatct gtcacaacct
     2401 ctcgtattca agctaatctt gattgctttg gaattgaact gagtcatgag gagagagaaa
     2461 ccttaaaaac gattgctgtt caatcgggtg ctccacgagt tgatgatgtg gatttctaga
     2521 aaatcataaa aagaattgta cattattcta atttttgata taatagtcag caggaaagaa
     2581 agtcttatgg cgttcttcaa gcgagcttgg gatagtggga gccaagtagg gcaaaataat
     2641 gagctggcgc tttctgtagt attttcaaaa acaatgaagt aataaattag ggtggaaccg
     2701 cgtttctgac gcccctaggt taaatcaacc taggattgtc ggacgcggtt cttttgctta
     2761 ttcagtctat tgtgtgaaag aaaggagagc cgtggacaac ctttatcttg taaaagacga
     2821 tagtcaacta gctacatttc gtgattttgt agtaagaaat actgaaaagt tgaaagatta
     2881 tcaatctttt ttaaagaatg aacttgcagt ctgtgattta ccgcaagccg ttatttggtc
     2941 agattttaat gctgctacac agattattag ggaaagcgct gttccagcct atacaaataa
     3001 tagacgaatg gttatggcgc ctgatttagc tgtttggaaa gagttgtatt tgtatcaatt
     3061 gatggactac gagtgttctc agcaaactca agcaatagaa agtcactatc attctttatc
     3121 tgaaaatttc ctcttacaga ttgtaggaca tgagttagct cattggtcgg agcatttttt
     3181 agatgatttt gatggttatg actcttatat ctggttcgaa gaggggatgg ttgaatatat
     3241 tagtcgcaag tatttcttga cagaagagga atttcaagcg gaaaaaattt gtaatcaatc
     3301 tctcgtagaa ctttttcaga agaagtatgg ttggcattca ttgaatgatt ttggttcttc
     3361 gacttatgat aagaactatg caagtatttt ttatgaatac tggcgcagct ttttgacagt
     3421 agataagttg gtagaaaatt taggtagtgt acaagcggtc ttagattctt atcatttatg
     3481 ggcaaataca gaaaaaactt ttcccttgtt agattggttt gttcagcaga aattaattga
     3541 aaaagaaata taaaaactaa aggagtaaac aatgtctaag aaattgacat ttcactgcgt
     3601 cagtggcaga aacctcctta cagtcggact gccccacgct cagcactaga gtgcctgagc
     3661 tagacgcagt actaactcgt cttgcctcgt atgatcgacg aggcagactc atgtcgcaag
     3721 taattatttt ttattaagga gtaaacaatg tctaagaaat taacatttca ctgcatcagt
     3781 ggcagaaacc tccttacagt cggactgccc cacgctcagc accctagtgc tgagctagac
     3841 gcagtactaa ctcgtcttgc ctcgtataat cgacgaggca gactcgtgtc gcaagaaatc
     3901 attttttatt aaggagtaaa caatgtctaa gaaattaaca tttcaagaaa ttattttgac
     3961 tttgcaacaa ttttggaatg accaaggttg tatgcttatg caggcttatg acaatgaaaa
     4021 aggtgcgggg acaatgagtc cttacacttt ccttcgtgct atcggacctg agccatggaa
     4081 tgcagcttat gtagagccat cacgtcgtcc tgctgacggt cgttatgggg aaaaccctaa
     4141 ccgtctctac caacaccacc aattccaggt ggtcatgaag ccttctccat caaatatcca
     4201 agaactttac cttgagtctt tggaaaaatt gggaatcaat cctttggagc acgatattcg
     4261 ttttgttgag gacaactggg aaaacccatc aactggttca gctggtcttg gttgggaagt
     4321 ttggcttgac ggaatggaaa tcactcagtt cacttatttc caacaagtcg gtggattggc
     4381 aactggccct gtgactgcgg aagttaccta tggtttggag cgcttggctt cttacattca
     4441 agaagtagac tctgtctatg atatcgagtg ggctgatggt gtaaaatacg gagaaatctt
     4501 tatccagcct gagtatgagc actcaaaata ttcatttgaa atttcggacc aagaaatgtt
     4561 gcttgaaaac tttgataagt ttgaaaaaga agctggtcgt gcattagaag aaggcttggt
     4621 acaccctgcc tatgactatg ttctcaaatg ttcacatacc tttaatctgc ttgacgcgcg
     4681 tggtgccgta tctgtaacag agcgtgcagg ctatatcgct cgtatccgta acttggcccg
     4741 tgttgtagcc aaaacctttg ttgcagaacg caaacgccta ggttacccac ttttggatga
     4801 agaaacacga gttaaacttt tagcagaaga cgcagaatag tgagtaatac tgtactctaa
     4861 aatcgttaga ggcaagtcga agacttgcta gagggatatg caccgccgta ctgttatgtg
     4921 gggatttttg aaaccttagg ttcaaaaatc agtcagctaa atccactttg atgagattgt
     4981 tggaaatctt gataaattta gcattggatt tccatacaga ctcacagagt tagttagcga
     5041 cgtccccagt acctaaggtg catatcaaaa aagattgaag aaaatgtaaa ggaaaaaaca
     5101 tgacaaaaaa cttattagta gaactcggtc ttgaagaatt accagcctat gttgtcacac
     5161 caagtgaaaa acaactaggc gaaaaaatgg cagccttcct caaggaaaac cgcctgtctt
     5221 ttgaagccat tcaaaccttc tcaacaccac gtcgtttggc tgttcgtgtg actggtttat
     5281 cagacaaaca gtctgatttg acagaagatt tcaagggacc agcaaagaaa attgccttgg
     5341 atagtgatgg aaactttacc aaagcagctc aaggatttgt ccgtggaaaa ggcttgactg
     5401 ttgaagatat cgaattccgt gaaatcaagg gtgaagaata tgtctatgtc actaaggaag
     5461 aagttggtca agcagttgaa gccattgttc ctggtgttgt agatgtcttg aagtcattga
     5521 ctttccctgt cagcatgcac tgggctggaa atagctttga atacattcgt cctgtccaca
     5581 ctttaactgt tctcttggat gagcaagagt ttgacttgga tttccttgat atcaagggaa
     5641 gtcgtgtgag ccgtggccat cgtttcttgg ggaaagaaac caaaattcag tcagcattga
     5701 gctatgaaga agaccttcgt aagcagtttg taatcgcaga tccatgtgaa cgtgagcaaa
     5761 tgattgttga ccaaatcaag gaaattgagg caaaacatgg tgtacgtatc gaaattgatg
     5821 cggatttgct gaatgaagtc ttgaatttgg ttgaataccc aactgccttc atgggaagtt
     5881 ttgatgctaa ataccttgaa gttccagaag aagtcttggt gacttctatg aaggaacacc
     5941 agcgttactt tgttgttcgt gatcaagatg gaaaactctt gccaaacttc atttctgttc
     6001 gtaacggaaa cgcagagcgt ttgaaaaatg tcattaaagg aaatgaaaaa gtcttggtag
     6061 cccgcttgga agacggagaa ttcttctggc gtgaagacca aaaattggtg atttcagatc
     6121 ttgttgaaaa attaaacaat gtcaccttcc atgagaagat tggttccctt cgtgaacaca
     6181 tgattcgtac gggtcaaatc actgtacttt tggcagaaaa agctggtttg tcagtggatg
     6241 aaacagttga ccttgctcgt gcagcagcca tttacaagtt tgacttattg acaggtatgg
     6301 ttggtgaatt tgacgaactc caaggaatta tgggtgaaaa atacaccctt cttgctggtg
     6361 aaactccagt ggtggcagct gctattcgtg aacactacat gcctacatca gctgaaggag
     6421 aacttccaga gagcaaggtc ggcgcagttc tagccattgc agacaaattg gatacgattt
     6481 tgagtttctt ctcagtagga ttgattccat caggttctaa tgacccttat gcccttcgtc
     6541 gtgcaactca aggtgtggtt cgtatcttgg atgcctttgg ttggcacatt gctatggatg
     6601 agctgattga tagcctttat gcattgaaat ttgacagttt gacttatgaa aataaagcag
     6661 aggttatgga ctttatcaag gctcgtgttg ataagatgat gggctctact ccaaaagata
     6721 tcaaggaagc agttcttgca ggttcaaact ttgttgtggc agatatgttg gaagcagcaa
     6781 gtgctctcgt agaagtaagc aaggaagaag attttaaacc atctgttgaa tcgctttctc
     6841 gtgcctttaa cctggctgag aaggcagaag gggttgctac agttgattca gaactatttg
     6901 agaatgacca agaaaaagct ttggcagaag cagtagaaac actcgtttta tcaggacctg
     6961 caagtcagca attgaaacaa ctctttgcgc ttagcccagt cattgatgct ttctttgaaa
     7021 atactatggt aatggctgaa gatcaggctg tccgtcaaaa tcgtttggca atcttgtcac
     7081 aactaaccaa gaaagcagct aagtttgctt gttttaacca aattaacact aaataaaatt
     7141 tgataaacgg actttatctt attacaaagg agaagaaatg gatccgaaaa aaattgctcg
     7201 tatcaatgag cttgctaaaa agaaaaaaac agaaggctta acaccagaag aaaaagtgga
     7261 acaagccaaa ctacgtgagg agtacatcga aggttatcgc cgcgctgttc gtcaccacat
     7321 tgaaggaatc aaaattgtgg acgaagaagg aaacgatgtt acaccagaaa aactacgcca
     7381 agtacaacgt gaaaaaggat tacatggccg tagtcttgat gacccaaatt cataatatgt
     7441 actctttctt ttaacaaaga tagatgaaac gataacaaag agactagcag tttgtgtttg
     7501 ctagtctttt ttcgctaaaa aaggaaccat aatggttcct aaaaactat
//
LOCUS       DAVBGY010000075         7394 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVBGY010000075 DAVBGY010000000
VERSION     DAVBGY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.075"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            225..6659
                     /locus_tag="VQ620_001853"
     CDS             225..6659
                     /locus_tag="VQ620_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET2764432.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALSISDKEKV
                     VVDKETENKEKHKDIHNAIETSKDTEEKKTTIIEEKEVVSKNPVIDTKTSNEEAKIKE
                     ENSNQSQGDYTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKGLSNLKNTKVLYT
                     YDRIFNGSAIETTPDNLDKIKQIEGISSIERAQKVQPMMNHARKEIGVEEAIDYLKSI
                     NAPFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDK
                     IPHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNA
                     QIFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRA
                     LRKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQ
                     TVEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLD
                     LRGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEG
                     TKSQVFSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNV
                     GDEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVI
                     NGKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIAL
                     QNTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYG
                     SISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDG
                     KQIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFES
                     VEEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGARSKTLGGYDDDG
                     KPKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNRTSLDQDPDLFAFNNQGVHAESTS
                     GSKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQKDLKVV
                     SREHFIRGILNSKGNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPAT
                     KIRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLI
                     TKDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTY
                     AGEGQGRNRKLDKDGNTIYEISGAGDLRGKIIEVIALDGASNFTKIHRIKFANQADEK
                     GMISYYLVDPDQDSSKYQKLGEIPESKFKNLKNVKDDSLNKETAEVENNLLVDNQSIE
                     GKSLFNIHKTISTIRDFENKDLKKLIKKKYKQEDDFVNGGTRTVERDYKYDDKGNIIA
                     YDDGTDLEYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKSIE
                     IKATKYDFHSKTMTFDLYANINDIVDGLAFAGDMRLFVKDNDQKKAEIKIRMPEKIKE
                     TKSEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKG
                     YALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRALSETTIYADSRNVEDGRST
                     QSVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDK
                     FNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNW
                     ELRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMD
                     GNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKR
                     NNGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDT
                     GEVSELKPHRITVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKK
                     DAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDL
                     QREDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIV
                     GIFLGLKKKNQD"
     gene            7285..>7394
                     /locus_tag="VQ620_001854"
     CDS             7285..>7394
                     /locus_tag="VQ620_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HET2764433.1"
                     /translation="MVLDYFFDKNLVFCLEADSQEQLFDQVATLLEEREIV"
BASE COUNT         2910 a         1013 c         1410 g         2061 t
ORIGIN      
        1 taatcttatt ttttaacgtg tgctaaaaaa taaattaatg catgttaatt tttttgactt
       61 aaattttttt aaatgatata ctgtttttat agagagataa caattatata taaatgtact
      121 actctatttc ctagataata cttagtaaaa ctttttataa caaaggctag caaagttaaa
      181 gtttattatc tattggaagt tatataaata tttaaggaat taaaatgaaa aaaagcacag
      241 tattgtcatt aactacagct gcagttattt tagcagccta tgcccctaat gaggtagtct
      301 tagcagacac atctagctct gaagatgctt taagcatctc tgataaagaa aaagtagtag
      361 tagataagga aacagaaaat aaagagaaac ataaagatat tcataatgct atagaaactt
      421 caaaggatac tgaagaaaag aaaacaacaa ttattgagga aaaagaagtt gttagtaaaa
      481 atcctgtaat agacactaaa actagcaatg aagaagcaaa aatcaaagaa gaaaattcca
      541 atcaatccca aggagattat acggactcat ttgtgaataa aaacacagaa aatcccaaaa
      601 aagaagataa agttgtctat attgctgaat ttaaagataa agaatctgga gaaaaagcaa
      661 tcaagggact atcaaatctt aagaatacaa aagttttata tacttatgat agaattttta
      721 acggtagtgc catagaaaca actccggata acttggacaa aattaaacaa atagaaggta
      781 tttcatcgat tgaaagggca caaaaagtcc aacccatgat gaatcatgcc agaaaggaaa
      841 ttggagttga ggaagctatt gattacctaa agtctatcaa tgctccgttt gggaaaaatt
      901 ttgatggtag aggtatggtc atttcaaata tcgatactgg aacagattat agacataagg
      961 ctatgagaat cgatgatgat gccaaagcct caatgagatt taaaaaagaa gacttaaaag
     1021 gaactgataa aaattattgg ttgagtgata aaatccctca tgcgttcaat tattataatg
     1081 gtggcaaaat cactgtagaa aaatatgatg atggaaggga ttattttgac ccacatggga
     1141 tgcatattgc agggattctt gctggaaatg atactgaaca agatatcaaa aactttaacg
     1201 gcatagatgg aattgcacct aatgcacaaa ttttctctta caaaatgtat tctgacgcag
     1261 gatctgggtt tgcgggtgat gaaacaatgt ttcatgctat tgaagattct atcaaacaca
     1321 acgttgatgt tgtttcggta tcatctggtt ttacaggaac aggtcttgta ggtgagaaat
     1381 attggcaagc tattcgggca ttaagaaaag caggcattcc aatggttgtc gctacgggta
     1441 actatgcgac ttctgcttca agttcttcat gggatttagt agcaaataat catctgaaaa
     1501 tgaccgacac tggaaatgta acacgaactg cagcacatga ggatgcgata gcggtcgctt
     1561 ctgctaaaaa tcaaacagtt gagtttgata aagttaacat aggtggagaa agttttaaat
     1621 acagaaatat aggggccttt ttcgataaga ataaaatcac aacaaatgaa gatggaacaa
     1681 aagctcctag taaattaaaa tttgtatata taggcaaggg gcaagaccaa gatttgatag
     1741 gtttggatct taggggcaaa attgcagtaa tggatagaat ttatacaaag gatttaaaaa
     1801 atgcttttaa aaaagctatg gataagggtg cacgcgccat tatggttgta aatactgtaa
     1861 attactacaa tagagataat tggacagagc ttccagctat gggatatgaa gcggatgaag
     1921 gtactaaaag tcaagtgttt tcaatttcag gagatgatgg tgtaaagcta tggaacatga
     1981 ttaatcctga taaaaaaact gaagtcaaaa gaaataataa agaagatttt aaagataaat
     2041 tggagcaata ctatccaatt gatatggaaa gttttaattc caacaaaccg aatgtaggtg
     2101 acgaaaaaga gattgacttt aagtttgcac ctgacacaga caaagaactc tataaagaag
     2161 atatcatcgt tccagcagga tccacatctt gggggccaag aatagattta cttttaaaac
     2221 ccgatgtttc agcacctggt aaaaatatta aatctacgct taatgttatt aatggcaaat
     2281 caacttatgg ctatatgtca ggaactagta tggcgactcc aatcgtggca gcttctactg
     2341 ttttgattag accgaaatta aaggaaatgc ttgaaagacc tgtattgaaa aatcttaagg
     2401 gagatgacaa aatagatctt acaagtctta caaaaattgc cctacaaaat actgctcgac
     2461 ctatgatgga tgcaacttct tggaaagaaa aaagtcaata ctttgcatca cctagacaac
     2521 agggagcagg cctaattaat gtggccaatg ctttgagaaa tgaagttgta gcaactttca
     2581 aaaacactga ttctaaaggt ttggtaaact catatggttc catttctctt aaagaaataa
     2641 aaggtgataa aaaatacttt acaatcaagc ttcacaatac atcaaacaga cctttgactt
     2701 ttaaagtttc agcatcagcg ataactacag attctctaac tgacagatta aaacttgatg
     2761 aaacatataa agatgaaaaa tctccagatg gtaagcaaat tgttccagaa attcacccag
     2821 aaaaagtcaa aggagcaaat atcacatttg agcatgatac tttcactata ggcgcaaatt
     2881 ctagctttga tttgaatgcg gttataaatg ttggagaggc caaaaacaaa aataaatttg
     2941 tagaatcatt tattcatttt gagtcagtgg aagaaatgga agctctaaac tccaacggga
     3001 agaaaataaa cttccaacct tctttgtcga tgcctctaat gggatttgct gggaattgga
     3061 accacgaacc aatccttgat aaatgggctt gggaagaagg ggcaagatca aaaacactgg
     3121 gaggttatga tgatgatggt aaaccgaaaa ttccaggaac cttaaataag ggaattggtg
     3181 gagaacatgg tatagataaa tttaatccag caggagttat acaaaataga aaagataaaa
     3241 atagaacatc tctagatcaa gatccagact tatttgcctt taacaaccaa ggtgttcatg
     3301 cagaatccac tagtggttct aagattgcta acatttatcc tttagattca aatggaaatc
     3361 ctcaagatgc tcaactagaa agaggactaa ctccatctcc acttgttcta agaagtgcag
     3421 aagaaggact aatttcaatt gtaaacacca acaaagaagg agaaaaccaa aaagacctta
     3481 aagttgtatc aagagagcac ttcataagag gaattctaaa ttctaaggga aatgacgcta
     3541 agggtatcaa atcatccaaa ctaaaagttt ggggtgactt gaaatgggat ggactcatct
     3601 ataacccaag aggtagagaa gaaaatgccc cagaaagtaa ggataaccaa gatccagcta
     3661 ctaagatacg aggacaattt gaaccgattg cggaaggtca atatttctat aaatttaaat
     3721 atagattaac taaagattac ccatggcagg tttcctatat tcctgtaaaa attgataaca
     3781 cagcccctaa gattgtttcg gttgattttt caaatcctga aaaaattaag ctaatcacaa
     3841 aggatactta tcacaaggta aaagatcaat acaagaatga aactttattt gctagagatc
     3901 aaaaagaaca tcctgaaaaa tttgatgaga ttgcaaatga ggtgtggtat gcaggagctg
     3961 ctcttgttaa tgaagatgga gaggttgaga aaaatcttga agtaacttat gcaggtgagg
     4021 gtcaaggaag aaataggaag cttgataaag atgggaatac tatctatgaa attagcggag
     4081 caggagatct aagaggaaaa atcatcgaag taatcgccct agatggagca agcaacttta
     4141 ccaaaatcca taggattaaa tttgctaatc aagctgatga aaaagggatg atttcctatt
     4201 atctagtaga tcctgatcag gattcatcta aataccaaaa gcttggcgaa attcctgaat
     4261 ctaaatttaa gaatttaaaa aatgttaaag atgatagcct aaacaaagag acagctgagg
     4321 tggaaaataa ccttttagta gataatcagt ctatcgaagg aaaatctctc tttaatattc
     4381 ataaaactat ttcaacaatt agagactttg aaaataaaga cctaaagaaa ctcattaaaa
     4441 agaaatataa gcaagaagac gactttgtaa atggtggaac aagaacagta gagcgtgatt
     4501 ataaatacga tgataaagga aatataatag cctacgatga tgggactgat ctagaatatg
     4561 aaactgagaa acttgacgaa atcaaatcaa aaatttatgg tgttctaagt ccgtctaaag
     4621 atggacactt tgaaattctt ggaaagataa gtaatgtttc taaaaatgcc aaggtatatt
     4681 atgggaataa ctataaatct atagaaatca aagcgaccaa gtatgatttc cactcaaaaa
     4741 cgatgacatt tgatctatac gctaatatta atgatattgt ggatggatta gcttttgcag
     4801 gagatatgag attatttgtt aaagataatg atcagaaaaa agctgaaatt aaaattagaa
     4861 tgcctgaaaa aattaaggaa actaaatcag aatatcccta tgtatcaagt tatgggaatg
     4921 tcatagaatt aggggaagga gatctttcaa aaaacaaacc agacaattta actaaaatgg
     4981 aatctggtaa aatctattct gattcagaaa aacaacaata tctgttaaag gataatatca
     5041 ttctaagaaa aggctatgca ctaaaagtga ctacctataa tcctggaaaa acggatatgt
     5101 tagaaggaaa tggagtctat agcaaggaag atatagcaaa aatacaaaag gccaatccta
     5161 atctaagagc cctttcagaa acaacaattt atgctgatag tagaaatgtt gaagatggaa
     5221 gaagtaccca atctgtatta atgtcggctt tggacggctt taacattata aggtatcaag
     5281 tgtttacatt taaaatgaac gataaagggg aagctatcga taaagacgga aatcttgtga
     5341 cagattcttc taaacttgta ttatttggta aggatgataa agaatacact ggagaggata
     5401 agttcaatgt agaagctata aaagaagatg gctccatgtt atttattgat accaaaccag
     5461 taaacctttc aatggataag aactacttta atccatctaa atctaataaa atttatgtac
     5521 gaaatccaga attttattta agaggtaaga tttctgataa gggtggtttt aactgggaat
     5581 tgagagttaa tgaatcggtt gtagataatt atttaatcta cggagattta cacattgata
     5641 acactagaga ttttaatatt aagctgaatg ttaaagacgg tgacatcatg gactggggaa
     5701 tgaaagacta taaagcaaac ggatttccag ataaggtaac agatatggat ggaaatgttt
     5761 atcttcaaac tggctatagc gatttgaatg ctaaagcagt tggagtccac tatcagtttt
     5821 tatatgataa tgttaaaccc gaagtaaaca ttgatcctaa gggaaatact agtatcgaat
     5881 atgctgatgg aaaatctgta gtctttaaca tcaatgataa aagaaataat ggattcgatg
     5941 gtgagattca agaacaacat atttatgtaa atggaaaaga atatacatca tttgatgata
     6001 ttaaacaaat aacagacaag acactaaaca ttaagattgt tgtaaaagat tttgcaagaa
     6061 atacaaccgt aaaagaattc attttaaata aagatacggg agaggtaagt gaattaaaac
     6121 ctcataggat aactgtgacc attcaaaatg gaaaagaaat gagttcaacg atagtgtcgg
     6181 aagaagattt tattttacct gtttataagg gtgaattaga aaaaggatac caatttgatg
     6241 gttgggaaat ttctggtttc gaaggtaaaa aagacgctgg ctatgttatt aatctatcaa
     6301 aagatacctt tataaaacct gtattcaaga aaatagagga gaaaaaggag gaagaaaata
     6361 aacctacttt tgatgtatcg aaaaagaaag ataacccaca agtaaaccat agtcaattaa
     6421 atgaaagtca cagaaaagag gatttacaaa gagaagatca ttcacaaaaa tctgattcaa
     6481 ctaaggatgt tacagctaca gttcttgata aaaacaatat cagtagtaaa tcaactacta
     6541 acaatcctaa taagttgcca aaaactggaa cagcaagcgg agcccagaca ctattagctg
     6601 ccggaataat gtttatagta ggaatttttc ttggattgaa gaaaaaaaat caagattaag
     6661 acaaaagcta tagaaaaaat ggtttatgta ctgagattag atagtgaggt gatgacatag
     6721 ttttgtgaaa acagccattt ataactcaat tatttagttt acttcactat tgatactatt
     6781 tggagttatt aatggactta gtttagataa ctaatgaatt gattgaaagg gttagtattg
     6841 acaatattgg tcatattgat tagaaaatag agtctatcaa aatttaaagg ctaatagagg
     6901 tgataagaca atttcaagga attaagtagc gtttggttgc ttaattcctt tttatttgga
     6961 aggaagtttt tcgtaaccgc ccaataacga agtctattgg acggttacga tatgatcact
     7021 gacttcgtca gtcttatcta caacctcaaa actgtgtttt gagcaacctg cggctagctt
     7081 cctagtttac tctttgattt tcattgagta ttagaacaga aaaaatgctt ggagtatttg
     7141 tttgtgtgtt tatttttaca taacaaacta taaacaaaac aaaaatataa aaaaagagac
     7201 aaaaaagaac agaaagtaat tgacaacggt ttcatatagt gttatactta tggcataaag
     7261 ttaatggaaa gaagggaaaa acttatggta ttagattatt tctttgacaa aaaccttgtg
     7321 ttttgcttag aagcggatag tcaagaacaa ctctttgatc aggttgcaac tttattggaa
     7381 gaacgagaaa ttgt
//
LOCUS       DAVBGY010000076         7170 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVBGY010000076 DAVBGY010000000
VERSION     DAVBGY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.076"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..516
                     /locus_tag="VQ620_001855"
     CDS             <1..516
                     /locus_tag="VQ620_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2764434.1"
                     /translation="GFTVSRTPLSSEDTVMIDATEVKINRPKKQLANDSGKKKFHAMK
                     AQAIVTSQGRIVSLDIAVNYSHDMKLFKMSRRNIEQAGKILADSGYQGLMKIYPQAQT
                     PRKSSKLKPLTAEDKAYNHALSKERSKVENIFAKVKTFKIFSTTYRNHRKRFGLRMNL
                     SAGIINHELGF"
     gene            complement(513..758)
                     /locus_tag="VQ620_001856"
     CDS             complement(513..758)
                     /locus_tag="VQ620_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET2764435.1"
                     /translation="MEQSPQKKKGTTLEHIILLRGVTPNGKNAIPKMSYLVDILTEAD
                     FQQVRTYIQSGNIILESNLALEEIREQVHTLLDFLQN"
     gene            complement(773..1945)
                     /locus_tag="VQ620_001857"
     CDS             complement(773..1945)
                     /locus_tag="VQ620_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET2764436.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSNPI"
     gene            complement(2004..2849)
                     /locus_tag="VQ620_001858"
     CDS             complement(2004..2849)
                     /locus_tag="VQ620_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2764437.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEDGGLLM
                     GYEID"
     gene            2974..3531
                     /locus_tag="VQ620_001859"
     CDS             2974..3531
                     /locus_tag="VQ620_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET2764438.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            3550..4017
                     /locus_tag="VQ620_001860"
     CDS             3550..4017
                     /locus_tag="VQ620_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HET2764439.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            4106..4735
                     /gene="upp"
                     /locus_tag="VQ620_001861"
     CDS             4106..4735
                     /gene="upp"
                     /locus_tag="VQ620_001861"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET2764440.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            4909..5499
                     /gene="clpP"
                     /locus_tag="VQ620_001862"
     CDS             4909..5499
                     /gene="clpP"
                     /locus_tag="VQ620_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HET2764441.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            5578..5826
                     /locus_tag="VQ620_001863"
     CDS             5578..5826
                     /locus_tag="VQ620_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HET2764442.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            5927..7087
                     /locus_tag="VQ620_001864"
     CDS             5927..7087
                     /locus_tag="VQ620_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2764443.1"
                     /translation="MKKKFALSFVALASVALLAACVEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTNMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
BASE COUNT         2259 a         1382 c         1495 g         2034 t
ORIGIN      
        1 ggttttacgg tttcaagaac tcctctcagt tctgaggaca cggtaatgat tgatgcgacg
       61 gaagtaaaaa tcaatcgccc taaaaaacaa ttagcgaatg attctggtaa aaagaaattt
      121 cacgctatga aggctcaagc gattgtcaca agtcaaggga gaattgtttc tttggatatc
      181 gctgtgaact atagtcatga tatgaagttg ttcaaaatga gtcgtagaaa tatcgaacaa
      241 gctggtaaaa tcttggctga cagtggttat caagggctca tgaagatata tcctcaagca
      301 caaactccac gtaaatccag caaactcaag ccgctaacag ctgaagataa agcctataac
      361 catgcgctat ctaaggaaag aagcaaggtt gagaacatct ttgccaaagt aaaaacgttt
      421 aaaatatttt caacaaccta tcgaaatcat cgtaaacgct tcggattacg aatgaatttg
      481 agtgctggta ttatcaatca tgaactagga ttctagtttt gcaggaagtc taatagagta
      541 tgaacctgtt ctcgtatttc ttcgagagct aagttacttt caagaataat atttccactt
      601 tgaatataag ttcgaacttg ttgaaaatca gcttctgtca agatatctac tagataagac
      661 attttaggga tagcattttt tccattagga gtgactcccc ttagcaagat aatatgttcc
      721 aaagtagttc ctttcttttt ttggggggat tgttccattt agtaatgtat atttagattg
      781 gatttgatcc cgccacccag ccggtacaga gggcagaagt gatgttaaag ccacctgtgt
      841 gggcattgat atccataact tcgcctgcaa agtggaggcc aggtaccagc ttactttcaa
      901 gggttttagg attgatttcc ttgagactga ctccaccctt ggtaacaaag gactttgcaa
      961 gggacatttt tccagttaca ggaattttaa gttctttaat ggactggaca agttgttctc
     1021 gttccttttc agtcagttgt ttgacttttt caggatatcc ttgtacaaaa aattcggcca
     1081 agcgttctgg taacaaggtt tttaaagcgt ttttcaagga tttttcccga ttttcttcta
     1141 gaaatgtaac caagtccttc tcagaaagtt gaggcaaaac atcgagtgag agaacctccc
     1201 cacctttgac aaagctagac atgcgtaggg cagcaggacc tgacaaacca aagtgggtaa
     1261 agagtaaatc atgagtgatg acatgcttac cataacttag ggtcacatcg tccaaagaaa
     1321 tcccttgtaa ggctttatgt ggaaaatctg ttaataaagg actttcagca gcctcaagat
     1381 cggtgatggt atgcttaaaa tggcgagcaa tctcgtgacc aaaaccagtc gaaccagtcg
     1441 aaggataaga cttaccacct gttgtgacaa tgagtttctc acaagtgaag gtttgatccg
     1501 ctgacttaag gacaaactgg tcatctactt ttttaacaga aacgatttct atttgagtag
     1561 caacttgacc acctagttcg gtgattttct tttccaaagc ttcgataata gtccgagact
     1621 tgtcactggc tggaaagacg cgtccgtggt cttcgacctt aagtttaaca ccattttctg
     1681 taaaaaagtt gatgatgtca tgattatcga actgggagaa aacactgtaa agaaagcgtc
     1741 cgtttccagg aattccagct agcaggttgt ctaagctacc attgttggtc acattgcaac
     1801 gtcccccacc agtcccagct aatttttttc caagtttccg atttttttcg atgaggaggg
     1861 ttttctgtcc ataaaagcta ctggaaatcg tagccatcat accagcaggt cccccaccga
     1921 tgacaatagt atcaaaatgt ttcatagctc tattgtacca caaaaaaaca agagatgatg
     1981 gtcacctctt gtcaagaata caattaatca atttcatagc ccatcagcaa accgccatct
     2041 tctgcataga aactgcagag accagaggtt ggtagaattt taatatccgc ttgtgggaag
     2101 gtttcacgga ttcgctctga gagctgttga caacattttt cgttattgcg ttgggccatg
     2161 acaatacggc caccagcata tccagctttt actaactcat cataggcagc ttgaactgat
     2221 ttctttgatc cccttgcttt ttgtagcaat tcgagagttc cagtttcact agcttttccg
     2281 accatacgaa tgttgagaag gccaacgacc gtaccgataa gcttgctcaa acggccgttc
     2341 ttcaccaagt tatcgacttt ggctaggaca aagagcaact tagttttttc ttgataggcg
     2401 gtgatagctt caaccacttc ttcaaaagac aagccctggt caatcaagtc attcaatttt
     2461 tctacgagta ggtcaacttc accaccagca gataaactat caatcacatg aatcttagtg
     2521 tcaggatggt cttccagata aatattcttt gctagttgag cactattgtg actgccagaa
     2581 agggtacctg tgatggttac taggaaaatg tttttggcac cttcaaatgc tcgcaaatag
     2641 tcatctgggc ttggacaagc cgattttgaa gcttctgcag ttgcatacat ggtttccatc
     2701 atttggtcaa tatcgagact ggcgtcatca acaaagacct gatcagctac ttgaatggtt
     2761 aaggggacac ttacaaaggt tgtgttaata gctggtgttg gcagttgacg ataatcacaa
     2821 ccagagtcag caataatctt ccaagtcata gaaattctcc atctttgtca gttatacatt
     2881 gacaaaggtt ctgtcttttt ttacaattat atcatgaaaa cccttgaaac aaaagcatca
     2941 tccgtctgtt gtgacaagta gaaagaaaat gttatgtctg aacgtagaat ctctgaaaag
     3001 tctcttgaaa atctcagaaa atcaaaccaa gaatccaatt tattaaccag agaagccatt
     3061 gaaacagccc tcttgcaact cttggaaaaa aaggaactga ccaagattag tatttctgaa
     3121 ttggtcaaac gtgcaggtgt ttcgcgtgcg gccttttatc gcaattatga ctccaaagag
     3181 gaaattttag agagcgtctt taaacgaact gtccacaata ttatggaaca gctgcatcat
     3241 tacgatttaa agacagacct ttatttggtt tgggttcacc ttttccggga ggccagaaag
     3301 gaagctagag taattcaatt ggccttggat taccatctgg aaaaaatctt tgtccaagcc
     3361 atgcaggaat ttctagaaaa ataccatggg aaatcaaaag gtgtcagctc ttatcttcat
     3421 tccttctgga gctcagccat cgtctctgtc cttctaaaat ggatcaagga tggcatgaag
     3481 gtaccagctg aaaagattgc agatttaggt ttaccatttt ttaaaaaata gagaaaaagg
     3541 agaaaagaga tgactgaaaa aagactggcc tgggatgagt attttgcagc ccaagctcta
     3601 ctaattgcga atcgttccac ttgtaaacgt gctaaagtgg gcgcgattct ggtaaaagat
     3661 aataaggtta tttccactgg ttacaatggt tcggtgtcag ggactgagca ttgtattgat
     3721 cacgaatgtt tggtcattga aggccactgt gttcgcaccc ttcacgctga ggtcaatgct
     3781 atccttcaag gtgctgagcg tggtgttccc aaaggcttta cagcctatgt aacccatttt
     3841 ccttgtctca actgtacaaa acaattgctt caggtcggct gcaagcgtgt ggtttatatc
     3901 aaccagtacc gaatggacga ctacgcccaa tacctttatc aagaaaaagg gacagaattg
     3961 acccatttac cacttgagac agtacagaca gctcttaaag aggctgatct aatgtaaaaa
     4021 ttatcaaaaa taaatggttt agaaagattt ttaacccgtt ttttggtata ataagaagaa
     4081 taaattgaaa gaaggaattc caaaaatggg aaaaattgaa gttattaatc acccactgat
     4141 tcaacacaaa ttgtcaatct tgcgtcgtac agatacttct acaaaagctt ttcgtgagct
     4201 agtagatgag attgcaatgt tgatggggta tgaagtactt cgtgatcttc cactagaaga
     4261 tgtggaaatc gaaacaccaa ttacaaaaac agttcaaaaa caattggcag gtaagaaatt
     4321 ggccatcgtc ccaatcttgc gtgcaggtat cgggatggtt gatggtctct tgagcttggt
     4381 tccagctgct aaagttggcc acatcggtat gtaccgtgat gaagaaacat ttcaaccagt
     4441 tgaatacttg gtgaaattgc ctgaggacat tgaccaacgt caaatttttg tagtagaccc
     4501 aatgttggca acaggtggct cagcaatctt ggctgttgat tctcttaaaa aacgtggcgc
     4561 atcaaatatc aaatttgtct gccttgtatc tgctccagag ggtgtaaaag cccttcaaga
     4621 agctcatcca gatgtagaaa tctttacagc agccttggat gaacgtttga acgaacacgg
     4681 ttatatcgtt ccaggtcttg gagatgctgg agaccgcttg ttcggtacaa aataagatcg
     4741 aaaagaagaa tgacttggaa aagtatttcc agtcacgaaa ggaggttggg tttttgtttc
     4801 tgtctaatga aagcagagca aaaatttgac cttttttgac caagatatta taatagtctt
     4861 atcttcagtc atttgaccaa caaaattaaa actcaaaagg agaaatgaat gattcctgta
     4921 gttattgaac aaacaagccg tggagaacgt tcttacgata tttactcacg tcttctcaaa
     4981 gaccgcatca ttatgctgac aggtccggtt gaagacaata tggctaactc tgttattgcc
     5041 caactgcttt tcttggatgc ccaagatagt acaaaagata tttaccttta tgtcaataca
     5101 ccaggtggtt ctgtttcagc tggtttggca atcgtagata ccatgaactt tatcaaggca
     5161 gatgtccaaa ccattgttat gggaatggct gcatctatgg gaacagtcat cgcatcaagt
     5221 ggagcaaaag gcaaacgttt catgcttcca aatgctgaat acatgattca ccaaccaatg
     5281 ggcggtacag gtggtggtac ccaacaaact gatatggcta tcgctgcaga acacttgctc
     5341 aaaactcgta ataccttgga aaaaatcttg gctgaaaatt caggtcagtc aatggaaaaa
     5401 gtccatgcag atgcagaacg tgataactgg atgagcgccc aggaaacact tgaatatggc
     5461 tttattgatg aaattatggc caacaattca ttgaactaat gatgatagaa ggcaaactcg
     5521 actgggtttg ctttttttgg tataataggg agagatttct tagaaagagg atttatcatg
     5581 tttgaaaaag tcaatcgctc tggcttgatt atctatcttt actataatcg tgatgccaaa
     5641 aaactgcagg attatggaga tattacctat cattccaaga aacatcgtta cttacaactc
     5701 tatgttccaa ctcaagaagt ggagcaattg gtcggacgct tgagcaagga aaaatttatt
     5761 aaaaaagtta gggtttgtca tatccaagag ttggaaacac cctttgtggg caatctttat
     5821 cgataggaaa acgttatcat cgaaaaagtt caaggaaaac attgacaatt ttctgataat
     5881 tcggtatatt cttaacagac tatttaagaa ataaggagac aaaaagatga agaaaaaatt
     5941 tgccctatcg tttgtggcgc ttgcaagtgt agcacttctt gcagcctgtg tagaagtgaa
     6001 gtctggagca gtcaacactg ctggtaactc agtagaggaa aagacaatta aaatcgggtt
     6061 taactttgaa gaatcaggtt ctttagctgc atacggaaca gctgaacaaa aaggtgccca
     6121 attggctgtt gatgaaatca atgccgcagg tggtatcgat ggaaaacaaa tcgaagtagt
     6181 cgataaagat aataagtctg aaacagctga ggctgcttca gttacaacta accttgtaac
     6241 ccaatctaaa gtatcagcag tcgtaggacc tgcgacatct ggtgcgactg cagctgcggt
     6301 agcgaacgct acaaaagcag gtgttccatt gatctcacca agtgcgactc aagatggatt
     6361 gactaaaggt caagattacc tctttattgg aactttccaa gatagcttcc aaggaaaaat
     6421 tatctcaaac tatgtttctg aaaaattaaa tgctaagaaa gttgttcttt acactgacaa
     6481 tgccagtgac tatgctaaag ggattgctaa atctttccgc gagtcataca agggtgaaat
     6541 cgttgcagat gaaactttcg tagcaggtga cacagacttc caagcagccc ttacaaacat
     6601 gaaagggaaa gactttgatg ctatcgttgt tcctggttac tataatgagg ctggtaagat
     6661 tgtaaaccaa gcgcgtggca tgggaattga caaaccaatc gttggtggtg atggattcaa
     6721 cggtgaggag tttgtacaac aagcaactgc tgaaaaagca tcaaacatct actttatctc
     6781 aggcttctca actactgtag aagtttcagc taaagctaaa gccttccttg acgcttaccg
     6841 tgctaagtac aatgaagagc cttcaacatt tgcagccttg gcttatgatt cagttcacct
     6901 tgtagcaaac gcagcaaaag gtgctaaaaa ttcaggtgaa atcaaggata accttgctaa
     6961 aacaaaagat tttgaaggtg taactggtca aacaagcttc gatgcagacc acaacacagt
     7021 caaaactgct tacatgatga ccatgaacaa tggtaaagtt gaagcagcag aagttgtaaa
     7081 accataatag aaaaatgttg aaatagggaa tgagcctttg actcactccc tgtttcgata
     7141 tttaatactc ttcgaaaatc tcttcaaact
//
LOCUS       DAVBGY010000077         7056 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVBGY010000077 DAVBGY010000000
VERSION     DAVBGY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.077"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(52..1113)
                     /locus_tag="VQ620_001865"
     CDS             complement(52..1113)
                     /locus_tag="VQ620_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET2764444.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMNLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            complement(1106..3937)
                     /gene="uvrA"
                     /locus_tag="VQ620_001866"
     CDS             complement(1106..3937)
                     /gene="uvrA"
                     /locus_tag="VQ620_001866"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET2764445.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            4069..5013
                     /locus_tag="VQ620_001867"
     CDS             4069..5013
                     /locus_tag="VQ620_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET2764446.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            5025..5708
                     /locus_tag="VQ620_001868"
     CDS             5025..5708
                     /locus_tag="VQ620_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HET2764447.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            5963..6994
                     /locus_tag="VQ620_001869"
     CDS             5963..6994
                     /locus_tag="VQ620_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET2764448.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDNNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
BASE COUNT         1984 a         1662 c         1438 g         1972 t
ORIGIN      
        1 aatttttttt gacttttttc gtcattttcg tacttttttc ttgacaaaca actaaatgac
       61 tatcaactct tttggagcta gggtcaataa ttcacaacct gtctctgtaa tcaggatatc
      121 atcctcgata cgaacgccat atttgccttc gatatagata cctggttcat cggtcaaggc
      181 catacctgtc ttaatagttt ctgtagaagt ctgactaaag tagggttcct catggatatc
      241 cagaccaata ccgtggccaa tgccgtgagt aaagtagtca ccataacctg cctcaatgat
      301 aatatcacga gggattttgt caaagtcacg gaaacctaag cctgccttag cttggtcaat
      361 caaggcttgg ttagctttta gaaccgtatt gtaaatctct gcctgctcat cgctaacatg
      421 ccctagatag atagtccggg tcatatcact gacatagtgg tcatagagac agccgaagtc
      481 catggtaatg gcttctccca actccactgg tttgtgcatt ggatgggcat ggggtttaga
      541 agaattgata ccgctagcta ggatcgtatc aaaagataag ccagatgctc ccaactcacg
      601 catgcggaaa tcaaggaagt tggcaatctc aatttcagtt tttcctggtt tgataaagtc
      661 aagcgcatcg cggaaagctt ggtctgagat agaacaagcc ttgcgaatcg ctgcaatctc
      721 tgcctcatcc ttaatcatac gaagaccttc cacaaactga gtttgtggaa gcaagttcaa
      781 acctgcaaaa gctgcctgca tacggtggta ataagacact gaaatctcat cttcaaaacc
      841 gatacgagtc aagttcatgt ccttaacaat tcctgcaatg acagccaatt catcacgatc
      901 agccacaatc tcaaaaccac tggtttcttg cttagctgcg atgatatagc gagagtctgt
      961 cactaagacc tgacggtcac ggctgataaa gactgttccg tttgagcccc aaaaaccagt
     1021 caaataatag acgtttttaa gattgttgat gatgatacca tctagttctt tttcttgcat
     1081 tttagctaga aatgcttgta cgcgtttatt catgatgtaa ctttcctttc aaatagtgtc
     1141 ctgtatagct ggcttcgttg gcagctactt cttctggagt tcctgttacg atgatggttc
     1201 caccaccgac accgccctca ggtcccaagt caatgatatg gtctgccgtc ttgataacat
     1261 ccagattgtg ctcgatgacg aggactgtat tgccgtcgtc tacaaagcga gctaaaacct
     1321 tgagcaggcg agcaatgtcc tctgtatgaa gccctgtcgt cggctcatcc agaatgtaga
     1381 aagattttcc tgtcgagcgt ttgtggagtt cgctagctaa cttcatacgt tgggcttctc
     1441 ccccagaaag ggtggtagct ggctgtccca aggtcacata gcctagacct acatccttga
     1501 tggtctggag tttgcgttga attttcggaa tgtgttggaa aaattctacc gcatcgttga
     1561 ccgtcatatc caagacctgc gaaatattct tttccttgta gtgaacttct agggtttcac
     1621 tgttatagcg ggttccgtgg caaacttcac aagccacata aacatctggc aagaagtgca
     1681 tctcaatctt gataatcccg tcacctgagc aagcttcaca gcgacctccc ttgacgttga
     1741 aactgaagcg ccccttcttg tagcctcgaa tcttggcttc atttgtctga gcaaaaaggt
     1801 cacgtatatc gtcaaaaact cctgtatagg tagctgggtt agacctcggc gtccgtccga
     1861 tagggctctg gtcaatatca atcaaacggt cgacatgctc aatccctgta atagtcttaa
     1921 acttaccagg tttgtctgaa ttacggttga gcttctgggc aatggctttt ttgagaatgc
     1981 tgttgattag agtcgatttc cctgaacccg acacacctgt cactgcgata aattttccta
     2041 gtggaaagcg agccgtgaca ttttgcaagt tgttctcacg cgctcctgtc acttcaataa
     2101 aacgaccatt tccgacacgg cgctcttctg gtactgggat gacacgtttg cctgacaagt
     2161 actgacctgt gatagacttg ctgttgcgag ccacttgctt aggtgtacct gctgcaacaa
     2221 tctcaccacc aaaaacaccg gcaccaggac caacgtcaat cagataatca gcttcacgca
     2281 tggtatcttc gtcgtgttcc accacgataa gagtattgcc caagtcacgc atctttttca
     2341 gactggcaat caggcggtca ttgtccctct ggtgaagacc gattgacggc tcgtctagga
     2401 tatagaggac acctgatagg ttggaaccaa tctgggttgc caaacgaatg cgctgacttt
     2461 ccccacctga aagggttcct gctgaacgtg acagggttag atagttaaga cccacattat
     2521 taaggaaggt caaacgatcc ttgatttcct tgagaatggg acgagcaatg atggcttcat
     2581 tttcagacaa agttaactgg ctcaccaagt ccaagtggtc agcgatagac aggtctgaga
     2641 tttctccaat atgtggccct tgctggccgc ccacacggac agacaaggcc tggtcattga
     2701 gacgatagcc ttgacaggtt ccgcaggtca gctcattcat gtagagacgc atctgagtgc
     2761 gagtgtaatc gctattggtt tcatggtaac gacgtttgat attattgata actccctcaa
     2821 acggaatgtc gatatcgcgc acaccaccaa attcattttc ataatggaaa tggaattcct
     2881 taccatctga gccgtagaga atcaagttct tatcttcttc tgacaggtcc tcaaaaggct
     2941 tatccatagc cactccaaag actttcatgg cctgctctaa catgtttgga tagtagttgg
     3001 atgagatagg attccaaggt gccagcgctc cctcatgtaa ggttttgctg gcatctggca
     3061 ctaccaaatc agtatccacc tccagcttaa tccccaagcc gtcacactca ctacaagagc
     3121 caaagggagc attgaaagag aagagacgag gttctaactc tgggacagta aatccacaaa
     3181 ctggacaggc ataatgctca gagaacaaca actcagagtc gtccatggtg tcgataatca
     3241 cataaccttc tgcgatacga agggcagctt caatggaatc aaagagacgg ctacgaatgc
     3301 cctccttgat aacaatacgg tcaaccacga catcgatatt gtgttgcttg ctcttagaca
     3361 actctggcac ttcggtcaca tcatagactt ccccatccac acgaacacga acatacccgt
     3421 ctttctgaac cttctcgata acactcttat gttggccttt tttcttgcgg atgacaggag
     3481 ccaagatctg caagcgctgg cgttcaggta actccaaaac cttatcaacg atttgctcca
     3541 cagaagaagc attgatagct ccatgtccgt tgatacagta aggcgtcccc acacgtgcgt
     3601 agaggagacg cagatagtca ttgatttcag tcgtcgttcc caccgtcgag cgaggatttt
     3661 tactagtcgt tttctggtcg atggaaatag ctgggctgag accatcaatg gcatctacat
     3721 ctggtttttc catatttccc aagaactgac gagcgtaggc ggacaaactc tctacatagc
     3781 gacgttgtcc ctccgcatag agggtatcaa aagccagact ggacttccct gaacctgaca
     3841 agccagtcac gacaaccaac ttgtctcgcg gaatctccac atcaatattt tttaaattat
     3901 gggcacgcgc cccatgaatg acaattttat cttgcatctt tgttctttct agtccattat
     3961 tgcttaccat tataccaaaa aaagtgagat tctattaccc aaaaggccga ttttgtagta
     4021 taatagtaca gtgtgaaaaa atctgaaaaa tgagaaagga taagggatat gaaacaagtt
     4081 tttctctcta caacaactga atttaaagag atcgatacgc ttgaaccggg tacttggatc
     4141 aatctcgtca atccgactca aaatgaatca ctcgaaatcg ccaacacctt cgatattgat
     4201 attgccgacc ttcgagcacc gctcgatgcg gaagaaatgt ctcgtattac cattgaagac
     4261 gagtataccc tgattatcgt agacgtgccg gtcacggagg aaagaaataa ccgcacctac
     4321 tacgtaacca tcccgcttgg tattatcatc actgaggaaa ccattatcac tacgtgtttg
     4381 gaaccactac ctgtccttga tgtctttatc aaccgtcgat tgcgtaattt ctataccttc
     4441 atgcgttcac gttttatctt tcaaattctt tatcgcaatg cagagcttta cctaacagcc
     4501 cttcgttcaa tcgaccgcaa gagtgaacaa atcgaaagtc aactgcatca atcaactcgt
     4561 aatgaagaat tgattgagct catggaattg gaaaaaacta tcgtctattt caaggcctcc
     4621 ctcaaaacaa atgagcgcgt gattaagaaa ttgaccagtt caaccagcaa tatcaagaaa
     4681 taccttgagg acgaagacct gcttgaagac accctgattg aaacccaaca ggccatcgag
     4741 atggcagata tttatggaaa cgtcttgcat tctatgacag agacctttgc ctctatcatt
     4801 tctaacaacc agaacaacat catgaaaacc ttggcccttg tgaccatcgt catgtccatc
     4861 ccaaccatgg tcttttctgc ctacgggatg aactttaagg ataatgaaat ccccctaaac
     4921 ggagagccaa atgccttctg gttaatcgtc tttatcgcct ttgctatgag tgtctcgctc
     4981 actctctatc tcatccataa aaaatggttc taagaggagt tcctatgtct caaattgatc
     5041 tacaaaaatt aactaagaaa aaccaagagt ttgtccacat tgctacccaa caattcatca
     5101 aagatgggaa aacagacgct gaaatccaga ctatttttga ggaagtcatt ccccaaatcc
     5161 ttgaggagca atctaaaggt acaactgccc gttccctata cggcgcacca actcattggg
     5221 ctcatagctt cactgtcaaa gagcagtacg aaaaagagca tccaaaagaa aatgatgacc
     5281 caaaactgat gattatggac tcagctcttt tcatcactag cctctttgcc cttgtcagcg
     5341 ccctcacaac cttctttgcg gcagatcaag ctttcggcta tggattgatt actcttctat
     5401 tagttggact ggttggtgga tttgccttct acttgatgta ctactttgtt taccaatact
     5461 atggaccaga tatggatcgc agtcaacgtc cacctttctg gaaatctgta ctagttatcc
     5521 tagcttctat gttcctttgg ttgcttgtct tctttgcaac aagcttccta ccagctagcc
     5581 ttaacccagt actggatcca ttgccactag ctattattgg agcagccctc ctagcccttc
     5641 gcttctatct caagaaacgc ttgaatatcc gtagtgcaag tgcaggacca acacgctatc
     5701 aagaataaga aaacgataaa agcaactgca ggtgcggttg ctttttcact tacttttttg
     5761 agttatactc aatgaaaatc aaagagcaaa ctaggaagct agctgcaggt tgctcaaagc
     5821 acagctttga ggttgcagat aaaactgacg tggtttgaag agattttcga agagtattaa
     5881 aagtattctt ctgaaatccc acatagcttt ctcttatatt ttgtgataaa ataggctcaa
     5941 tctatttcta ggaggatgag atatggtttc tactattggt attgttagtt tatctagtgg
     6001 cattatcgga gaggattttg tcaaacacga agtggacttg ggtatccaac gtctcaagga
     6061 tctgggactc aatcccatct ttttgcccca ttcgttaaaa ggattagact ttatcaagga
     6121 ccatcctgaa gctcgtgcag aggatttgat tcatgccttt tctgatgata gcatcgacat
     6181 gatcctatgt gccatcggtg gagacgatac ctatcgcttg ctaccttatc tttttgaaaa
     6241 tgaccaactc caaaaggtta tcaagcaaaa aatttttctt ggcttctcgg atacaaccat
     6301 gaaccacctc atgttgcata aattaggaat caagactttt tatggtcaat cctttttagc
     6361 agacatttgt gaattagaca aagaaatgct agcctatagc cttcactact ttaaagaatt
     6421 gattgagacg ggaagaattt cagaaatccg ccctagtgac gtttggtatg aggaacgaac
     6481 tgattttagt cccacggccc tgggaacacc tcgtgtcagt catacaaata caggttttga
     6541 cttgttgcaa ggaagtgccc agttcgaggg gaaaatcctc ggtggttgcc tcgaatccct
     6601 ctacgatatc tttgacaact ctcgatacgc agatagcacg gagctctgcc aaaaatacaa
     6661 acttttccct gacttgtcag actgggaagg gaaaatcctc ttgctcgaaa caagcgaaga
     6721 aaagcctaag ccagaagact tcaaaaagat gttgctaact ttaaaggaca ctggcatatt
     6781 cgcggtcatc aatggactct tggtcggaaa acctatggat gaaactttcc atgacgacta
     6841 taaagaggca ctattggata tcattgacaa caatatcccg attgtctata atctgaatgt
     6901 cggccacgca actccaagag caattgtacc ctttggggtc catgctcatg tagatgcaca
     6961 ggaacaagtc attcgctttg actataacaa ataaagctgg gaaaagtttc tcagcttttt
     7021 tctcggtatc attactggca ttttcatcac atgtta
//
LOCUS       DAVBGY010000078         7051 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVBGY010000078 DAVBGY010000000
VERSION     DAVBGY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7051
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.078"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(201..989)
                     /gene="argH"
                     /locus_tag="VQ620_001870"
                     /pseudo
     CDS             complement(201..989)
                     /gene="argH"
                     /locus_tag="VQ620_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
     gene            complement(1049..2245)
                     /locus_tag="VQ620_001871"
     CDS             complement(1049..2245)
                     /locus_tag="VQ620_001871"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET2764449.1"
                     /translation="MSKEKVILAYSGGLDTSVAITWLKKDYDVVSVCMDVGEGKDLDF
                     IHDKALKVGAVESYVIDVKDEFATDYVLVAHQSHAYYEQKYPLVSALSRPLISKKLVE
                     IAHQIGATTIAHGCTGKGNDQVRFEVSIAALDLNLKVIAPVREWKWSREEEIYYAKEN
                     GVPVPADLDNPYSVDQNLWGRANECGILENPWNQAPEEAFGITTSPEQAPDMPEYIEI
                     EFSEGVPVSLNGEVLKLADLIQKLNEIAGKHGVGRIDHVENRLVGIKSREIYECPGAV
                     TLLTAHKEIEDLTLVREVAHFKPIIENELSNLIYNALWFSSATQALIAYIKETQKVVN
                     GTAKVKLYKGSAQVVARKSPSSLYDENLATYTSADTFDQDAAVGFIKLWGLPTKVHSE
                     VQKSAK"
     gene            complement(2262..3068)
                     /locus_tag="VQ620_001872"
     CDS             complement(2262..3068)
                     /locus_tag="VQ620_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2764450.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSIPYYTAKNKLIVKKSDLTTYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVSKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            complement(3275..3916)
                     /locus_tag="VQ620_001873"
     CDS             complement(3275..3916)
                     /locus_tag="VQ620_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2764451.1"
                     /translation="MIELENITKTIGGKVILDHLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLSKKDKLKKVEEILERVGLSSTLHQKVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGEGK"
     gene            complement(3913..6021)
                     /locus_tag="VQ620_001874"
     CDS             complement(3913..6021)
                     /locus_tag="VQ620_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2764452.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLLFNYLMKIGTTILLTVL
                     LVGVGKSLEQENKELDYQKQWISQGNYLTLETFQLNDNLWQEQLAGSGKALDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIANTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     VLASRMEKRSLVELLKGGIL"
     gene            complement(6073..6360)
                     /locus_tag="VQ620_001875"
     CDS             complement(6073..6360)
                     /locus_tag="VQ620_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2764453.1"
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFG
                     YSDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            6786..>7051
                     /locus_tag="VQ620_001876"
     CDS             6786..>7051
                     /locus_tag="VQ620_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HET2764454.1"
                     /translation="MQRLEVYKNYQHLYDLQIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELIEETS"
BASE COUNT         2164 a         1591 c         1143 g         2153 t
ORIGIN      
        1 ctgccaagcc acatagtgtg tttactacta tgtgtccatg tcaccatgaa tcgccgaacg
       61 atttaacgag aaagtatgac ttttacgttt atcccaactc aattatgaca tttttttcaa
      121 aagtcaatat atctcacttt ttcaacgaca agaaagaggc tgccaatcga ccaacctctt
      181 attctgaacc catcactcca tcacttttta gcttcattcg ctttcttagc gactgcaatc
      241 tggtattcga cttggtcaaa ccctgtgcct cccaaggaat ttcgtctttg aacagcagtt
      301 ttaggttgca agtagacata gatatcctcg gtaatgagag aatgataggt ttgtaattcc
      361 tccaaagtcc aatcttgaag atttttagca gacttgatag agtctagcac taatcttccc
      421 acaacctcat gagcttctct aaatggcaag cctttccctg ccaaataatc tgccaactca
      481 gttgcatttg aaaagtcctt ctctgttgat tcttgcattt tttccttgtt tacctgcaaa
      541 ctagataaca tacctgccaa cacatcgagg gaatttaaaa tcgtttcgac cgtatcaaac
      601 attccttcct tatcttcttg caaatcctta ttataagcta aaggcaagga cttcatgact
      661 gtcaacagtc caaacaaatg cccataaact ctgccagtct ttcctcgaat caactcagcc
      721 atatctggat ttttcttttg gggcataata gaggaacctg ttgtaaacgt atctgacaag
      781 gtaatgaatt ggtactcaaa actacaccaa ttgatcattt cttcgcaaaa acgactcata
      841 tgcatcatga gtatgctggc atttgataga aattctaaga taaaatcgcg gtcactaaca
      901 gcatccaaag aatttgtata aggttgctta aactccagca aatcgctaga caattggcga
      961 tcaatgggga aagtcgttcc tgccaaagcg accaccccat aattttgtat tcttagccat
     1021 atcacatcca cctctctatc caatcgtcct atttggcgct tttttgaacc tcagaatgaa
     1081 ccttggttgg aagtccccat aacttaataa agccaacagc cgcatcttgg tcaaaggtat
     1141 ctgcactagt atacgtcgcc aaattttcat cgtaaagaga acttggtgat ttccgagcca
     1201 cgacctgagc gctcccctta tacagtttga cttttgcagt cccattgaca actttttgag
     1261 tctctttaat ataagcaatc aaggcttgtg tagccgaact aaaccacaaa gcattataaa
     1321 tgagatttga taattcattt tctatgattg gtttaaaatg agccacttct ctcacaagcg
     1381 tcaagtcctc aatttcctta tgagctgtca gcagggtcac tgcacctgga cactcataga
     1441 tttctcttga tttaatccca accaagcgat tttccacgtg gtcaatccgt ccaaccccat
     1501 gttttccagc tatttcattg agtttttgaa tcaaatctgc caattttaac acttctccat
     1561 tgagggaaac tggaacacct tcactaaact caatctcaat gtattctggc atatctggag
     1621 cttgctctgg agaagttgtg atgccaaagg cttcttctgg agcctgattc catggatttt
     1681 ccaaaatacc acactcattg gcacgtcccc aaagattttg gtcgacagag tagggattgt
     1741 caaggtcagc aggaacaggc accccatttt ccttggcata ataaatttct tcctcccgag
     1801 accatttcca ttcacgaact ggagcaatca cttttaaatt gagatccaag gctgcaatcg
     1861 atacttcaaa acgtacttgg tcattcccct taccggtaca accatgagca attgtagtcg
     1921 ctcctatctg atgcgctatt tcaaccaatt ttttagaaat cagagggcgg ctcaaggcag
     1981 ataccaaggg atacttttgt tcataatagg catgtgactg atgagccact agcacataat
     2041 ctgtagcaaa ttcgtcctta acatcaatga cataagattc tactgcccca accttaagag
     2101 ccttatcatg gatgaaatct aagtctttcc cttcacccac atccatacaa actgaaacaa
     2161 catcatagtc tttctttaac caggtaatag caactgatgt gtccaaaccg ccagaatagg
     2221 ccaaaattac tttttcttta ctcatgttcc tttccttctt tctatttttc aatggatgct
     2281 ttaaaggcat cctcaatgag tttgtctaat tccccagact ccttcaactt ttgaatggtt
     2341 ttatcgactg cctctttcaa ttccttgcta tcttttttca tggctaccgc gtaggaatca
     2401 tcttgctctt tttcaaaatt gaggtctgcg attgctaaat caggattatt ttccacaaat
     2461 cccttggaaa caggttcttc aaagataacg gcatccactt gtcctgattt taaatctgtg
     2521 attaaattcc catttttagg cagagatacg agggaagaat tttgtagcaa atctttcgcc
     2581 atcgtctctt gaatcgaacc tttctgcgct ccaacctttt tctgcgccaa gtcgtttaca
     2641 gactgataag tagtcaagtc agattttttg acaatgagtt tattttttgc agtatagtag
     2701 ggaatggaaa agtcaaacac cttgctccgt tcatctgtct tagaaacacc tgatatggca
     2761 aggtcggctt ttcctgattg aacactagcc agtacattat caaaactcat gggagatagt
     2821 tccaattcga cacctagttc tgttgcgata gccttggcta attcgatatc tgaacccaca
     2881 atctgatttt tcccatcaac cactttttgg tattcaaatg gagcaaaatc tggattgagg
     2941 gctacaacca attttccttt ggacttgatg gcttcaattc cagccgattg attgttgcta
     3001 caagcaaata gtagggggag catcactaaa ccaaacatcg tcatcaacac cttcttcatc
     3061 ttattcataa caaagtctcc tttatgttta ttctttttgg ttgtataaat aatacaccta
     3121 tcttcatcct ttgtcaagcc taaattggaa aaattttcaa tttgaaacaa aaaaacctag
     3181 aaatactaca taaaaatgtc atttctaggc gtatttatgc tattcctctc tgaaaaacat
     3241 gagtattcag tcagtcaaaa agagctgaac aaactcattt cccctcgccc aattcaatga
     3301 tccgatgaca ttgttgggct acataagcat cgtgggtcac aataatgact gttttcccct
     3361 cgcgattcat ctctaagaga aacttcaaga ccaaatctct attttcagga tctagcgaac
     3421 ctgttggttc atcggctaaa atcagctggc tgggttttaa gatggctcta gcaactgcaa
     3481 ttcgttgttg ttcgccacca gacaactcgg aaaccttttg atgcaaagta gatgacaaac
     3541 ctactctctc taaaatctct tccacctttt tgagcttgtc tttcttagac aatttcacat
     3601 atttcagcgc cagcatgaga ttgtactcga ccgtttcatc atcaatcagg gcaaaatttt
     3661 gaaacagata agagatatgt tcacggatta ttgtttgcga cttagcagaa ttgaccgcta
     3721 gatttgtctg accaaaaatc tcataccgtc cactataatc accatctatc aaacctaata
     3781 aatttaacaa ggtcgacttc ccactaccac tttttccaac aatagctacc aaatccccct
     3841 gatcaatcct gagagacaag tgatccaaaa tcacttttcc cccaatggtt ttggtaatat
     3901 tttccaactc aatcataaga tgcccccttt caataactct actagacttc ttttctccat
     3961 cctagaagcc aagactagca caaatagtat atccaaacat gtaaagcctg caaacagcag
     4021 aagtggcaag aacgcatggg caaagaaaat caagactaga agagggaaac tatagcccag
     4081 caagagcaga acgaggagag gacggtagcg atcgaccagt ttccacccca taaacttctt
     4141 ggtaatgata tccctgcgct tcaataagaa agttgttact agtaagaagt aggaaatcat
     4201 catgctaagg agaccaaaca aagcaaagag taggttaaaa ttccgaacag catctcgata
     4261 agaatccact ttctcttgtt gaatggcttg aatagatgaa aattttaaat aatttccatc
     4321 tgacaatttc tcaactaact ctgtaatctc tttttgatgt tgaaccgtat ttgcaatttt
     4381 aatcggatta tttaagccag ttgttgacag ggaggctttc tcatcccaca tcatatcaga
     4441 atcattgacc aagctaataa ttggattgga gagattttcc tttcgcttat cactatatgg
     4501 gaaaaatgac caatctcctt cataataggc aatctcgaca tccatctcct ctatcgttcg
     4561 tttttgctgc tcttcatact tcatcgaatg aaaggcaatt aacttcccca agagctgatt
     4621 tttatcttct tcacctttcg tacttgctgg catcaaaata acttttttaa taccggtatc
     4681 tggtagcttg aatcccttgc tctttagaaa attgcgattg gcatagtaaa catccaccgt
     4741 atctgttaac tgatattgct gaatctgttc tgattggaca aaatttttta caggaagact
     4801 gctactctgc acatagcccg cctgcgtttt ttctaccaaa tcctgataaa atcgatagaa
     4861 ataatccaat gccttccctg agcctgccaa ctgctcttgc cacaagttat cattgagttg
     4921 aaaagtttct aaggtcagat aatttccttg gctgatccac tgtttctggt aatccaactc
     4981 tttgttttct tgctccaaac tttttcctac cccaactagc aagactgtca gtaaaatggt
     5041 agttccaatt ttcatcaaat aattaaagag tagaccaaat ttgaaagatg aaaaaccttt
     5101 cagcagagag ctgattgtca ttttttggat taagaggtaa gtcaaccaac tgataaagag
     5161 ataaagctgc aacagcaaaa aatgagacaa ccacagcata ggaaacaaat cttttggctt
     5221 ataatcaagc aagaaaaaca cgcctagatt gatcacaaga gccccaccta ggaggaggta
     5281 aaggttgcct tttacaacat cagctaaaac agccctatct tgaaaaccaa gtaatttttg
     5341 taccccaact cttttcatct ccatcatcgg ttgatacact gtcactaaca caagaagcaa
     5401 aatagccaag acaaaaacaa tggcagataa aagcaaatct cgatttatga cttccactgc
     5461 acttttgtag gtcggctcta gcaaggtagc ctggtctatc ttgaaaaaat cgctccattt
     5521 ctgtacaatc ctatccttgt ccatctcttg tgtagaagtt atcgtatagc gaccatttaa
     5581 actacgagat gtatccttga tataggtttg aaaagtcata agctgaatag gtttggcttt
     5641 tagaaaggtc ggaatcgtac caagtttatt ggaaatttct ttattactat agactccttc
     5701 accatctgtg gtaaaatcaa gagaagaaat cccaaactct tggtagggga aggtatcttt
     5761 atcaaaaaca ccagacttga ccacctcatc accactgtct gttttgatga tggagacttt
     5821 atactccttt gatacatcct caaaaaatcg aagaacagac gctgcaggtt cgttaatatc
     5881 tttcaaatac aaatccaaag aatctacagt ctttcccatt tcttgaatcc gaaccacttc
     5941 tcgtgtatag tttacattaa aaacaaaaaa agtggtacac agaagcagga gtaacataca
     6001 gaaattactg atttttttca taaaagtgcc tccactacag ttttccctct ccaaatagga
     6061 gagggaagaa ttttactcaa atccatagaa atattccatt cctgttggcg gaatcttggt
     6121 gagggaagct ctagcccatg cctcaggttt tgcataatcc ttagaggttc tacccccatg
     6181 tctaacagtt gctgtatgat atcgagtaga atgtaagtaa tcagaatatc caaaagttcc
     6241 tgtccaacct actccatagt tccattgacc accctcaacc catactgcta aagcatttgt
     6301 agtagcaagt ataccacata ccattaaaga tgcaacacca attttaacta attttctcat
     6361 gatttatttc tcctattatc gagagctgtt ccgtaaaaac agctatagta atttatcaac
     6421 aagtatcgta gtccattgga ctcaccttcc ttctatctca tttcattttt taacaacgaa
     6481 cttactctta accgggagaa tggtgcgctt ctgtatcagg tccttgaaca accagagtag
     6541 tattaagcac actctaacta ttactcaaga atcagtccaa taaacaatat ggatagcgct
     6601 ttcaaatttt taaactctta tcccatcctt ttatctatat aataagtgaa aatataataa
     6661 ctgtcaagta atcgaagtga attttataaa aaaaattaca agccaaattt gtaaagttta
     6721 cactaagccg ctagggcaat cgtctatcag aatgtccgtt tatttgtcaa taatccgaga
     6781 aaatcttgca acgcttagaa gtctataaaa actatcaaca tttatatgac ttgcaaatag
     6841 caatcctgct caacctttcc acactctatc tatacaatca agataaaaac atgtgtaagc
     6901 aaatctgcta cactttacta gaggacgcca agaataagaa aagctacgat aggctggcta
     6961 tctgctatgt ccgtattggg atttgtacag acgattctaa acttatccaa aaagggttct
     7021 cccttctgga gctaatcgag gaaacttcta t
//
LOCUS       DAVBGY010000079         7049 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVBGY010000079 DAVBGY010000000
VERSION     DAVBGY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7049
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.079"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(353..2044)
                     /locus_tag="VQ620_001877"
     CDS             complement(353..2044)
                     /locus_tag="VQ620_001877"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET2764455.1"
                     /translation="MNTKELIASELASIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            2180..2626
                     /gene="argR"
                     /locus_tag="VQ620_001878"
     CDS             2180..2626
                     /gene="argR"
                     /locus_tag="VQ620_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2764456.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            2677..5211
                     /gene="mutS"
                     /locus_tag="VQ620_001879"
     CDS             2677..5211
                     /gene="mutS"
                     /locus_tag="VQ620_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET2764457.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKLDSQNNFLVAIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSCQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPALAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLITGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTENPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            5358..>7049
                     /locus_tag="VQ620_001880"
     CDS             5358..>7049
                     /locus_tag="VQ620_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2764458.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDVPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
BASE COUNT         1912 a         1374 c         1675 g         2088 t
ORIGIN      
        1 atgaactagg attctagttt tgcaggaagt ctattatttg gttaggtgaa ttagtgaagc
       61 gtttaggcaa gtgtctctgg ttacgacgtc atggactcta aatcgattat atttaggggt
      121 catgactagt gaagcagtta gctagttcgc atataagcgg ctagcgtcta acaattagga
      181 actttagttc caataacttt aagattacga cgttttagga cataaatcga tcatatttat
      241 gtcctaaaac tagtgaagta tgtcctaaaa ctagtgaagc gcctagccaa agtccgaata
      301 ggatttggcg ttagttactt agattgcttt gcaatcaagt aactttggcg atttacatct
      361 tctctggcgc ttctactcca agcaagcgaa gggcttcttt gagaacgact gcggttgcgt
      421 agctgagggc tagacggctg tcgcgttctg ggctttcatc caagatacgt gtatgtgcat
      481 agtatttgtt aaaggattga gccaggctaa ttgcaaattt agcaatgata gaaggttcaa
      541 agttatctgc cgcacggttg ataatacgtg ggaagtcttg aatgagttta atgatttccc
      601 agctttcagt atcattcaag ctatagttgc cagctgtttc tggtttgaaa tcggctttgc
      661 gtaagataga ttggatacga gcgtaggcat attgaacgta aggtccagtt tcaccctcga
      721 aggataccat agcctctagg tcgaagtcgt atccatttgt acggtcggtt ttgaggtcat
      781 agaatttaat ggctccaacc ccaacagcat gtgctacttg gtctttgttt tctagttcag
      841 gatttttagc ctcgatttgg accttggcac ggctaacagc ctctgcaaca gtaggctcta
      901 gcaagatgac attcccttta cgagtagaga gtttcttccc ttcttttgta accaaaccaa
      961 aaggaacgtg agtaatgtcg tcactccagt cgtagcccat ctcttgcaag acagctttga
     1021 gctgtttaaa gtgggcagat tgttcttgac caacgacata gatagattta gcaaattggt
     1081 attcgttttt acggtagagg gctgcagcca agtcacgtgt gatatagaga gttgcaccat
     1141 cagacttctt gatgagggct ggatgttcaa ttccgtattt ctcaagattc acaacttggg
     1201 caccttctga ttcaagaagt agtccttttt cagaaagaat gtctacaact gcatccatct
     1261 tatcattgta gaaggcttct ccgttatagc tgtcaaattc aaccttcaat tcattgtaaa
     1321 ggcggttaaa ttccactaaa ctttcatcgc ggaaccattg ccaaagagcg agagcttcct
     1381 catctccatt ttcaagttta cggaaccatt cgcgcgcttc ttcatccaag ctagggtcat
     1441 tttcagcttc agcgttgatg cggacataga gtttaaggag ttcatcgatt ggatgagctt
     1501 ttacagcttc ttcgtcgccc cattttttgt aggcaacaat caacatccca aattgtttac
     1561 cccagtctcc caaatggttg accttgaccg tttgataacc gattttttgg aaaatatgtg
     1621 acaagctatc tccgataaca gttgaacgca ggtggccaat agaaaatggt ttagcgatat
     1681 tcggactaga catgtcgata acaacatttt cttgtttacc aatattttgg tcagcatagt
     1741 gttctttttc agtggtaaca gcttgcaata cttgagcaga aatggcagat ttatcaagga
     1801 aaaagttaac gtaaggtcct gttgcgacaa ctttttcaaa ggcttggctg ttcatttttt
     1861 cagccagttc agccgcaatc atttgtggtg ctttacgttc gactttggca agagaaaaag
     1921 cagggaaagc gatgtcaccc atttctgagt ttttaggggt ttccagtaac tttaaaatag
     1981 cctcttggtc caggctatca atgatgctag ccaattcgct agcaatcaat tcttttgtat
     2041 tcattaagag ctcctttttg gacttttcta ctattttatc acaattttaa agaaagaaga
     2101 aaaaattttt gaaatctcct gtttttttgg tataatatgg ttataaatat agttataaat
     2161 atgcacgcaa gaggatttta tgagaaaaag agatcgtcat cagttaataa aaaaaatgat
     2221 tactgaggag aaattaagta cacaaaaaga aattcaagat cggttggagg cgcacaatgt
     2281 ttgtgtgacg cagacaacct tgtctcgtga tttgcgcgaa atcggcttga ccaaggtcaa
     2341 gaaaaatgat atggtgtatt atgtactagt aaatgagaca gaaaagattg atttggtgga
     2401 atttttgtct catcatttag aaggtgttgc aagagcagag tttaccttgg tgcttcatac
     2461 caaattggga gaagcctctg ttttggcaaa tattgtagat gtaaataagg atgaatggat
     2521 tttaggaaca gttgctggtg ccaatacctt attggttatt tgtcgagatc agcacgttgc
     2581 caaactcatg gaagatcgtt tgctagattt gatgaaagat aagtaaggtc ttgggagttg
     2641 ctctcaagac ttatttttga aaaggagaga cagaaaatgg cgatagaaaa gctatcaccc
     2701 ggcatgcaac agtatgtgga tattaaaaag caatatccag atgctttttt gctctttcgg
     2761 atgggtgatt tttatgaatt attttatgag gatgcggtca atgctgcgca gattctggaa
     2821 atttccttaa cgagtcgcaa caagaatgcc gacaatccga tccctatggc gggtgttccc
     2881 tatcattctg cccaacagta tatcgatgtc ttgattgagc agggttataa ggtggctatc
     2941 gcagagcaga tggaagatcc taaacaagca gttggggttg ttaaacgaga ggttgttcag
     3001 gtcattacgc cagggacagt ggtcgatagc agtaagctgg acagtcagaa taattttttg
     3061 gttgccatag accgcgaagg caatcaattt ggcctagctt atatggattt ggtgacgggt
     3121 gacttttatg tgacaggtct tttggatttc acgctggttt gtggggaaat ccgtaacctc
     3181 aaggctcgag aagtggtgtt gggttatgac ttgtctgagg aagaagaaca aatcctcagc
     3241 tgccagatga atctggtact ctcttatgaa aaagaaagct ttgaagacct tcatttattg
     3301 gatttgcgat tggcaacggt ggagcaaacg gcatctagta agctgctcca gtatgttcat
     3361 cggactcaga tgagggaatt gaaccacctc aaacctgtta tccgctacga aattaaggat
     3421 ttcttgcaga tggattatgc gaccaaggct agtctggatt tggttgagaa tgctcgctca
     3481 ggtaagaaac aaggcagtct tttctggctt ttggatgaaa ccaaaacggc tatggggatg
     3541 cgtctcttgc gttcttggat tcatcgcccc ttgattgata aggaacgaat cgtccaacgt
     3601 caagaagtag tgcaggtctt tctcgaccat ttctttgagc gtagtgactt gacagacagt
     3661 ctcaagggtg tttatgacat tgagcgcttg gctagtcgtg tttcttttgg caaaaccaat
     3721 ccaaaggatc tcttgcagtt ggcgactacc ttgtctagtg tgccacggat tcgtgcgatt
     3781 ttagaaggga tggagcaacc tgctctagcc tatctcatcg cacaactgga tgcaatccct
     3841 gagttggaga gtttgattag cgcagcgatt gctcctgaag ctcctcatgt gattacagat
     3901 gggggaatta tccggactgg atttgatgag actttagaca agtatcgttg cgttctcaga
     3961 gaagggacta gctggattgc tgagattgag gctaaggagc gagaaaactc tggtatcagc
     4021 acgctcaaga ttgactacaa taaaaaggat ggctactatt ttcatgtgac caattcgcaa
     4081 ctgggaaatg tgccagccca ctttttccgc aaggcgacgc tgaaaaactc agaacgcttt
     4141 ggaaccgaag aattagcccg tatcgaggga gatatgcttg aggcgcgtga gaagtcagcc
     4201 aacctcgaat acgaaatatt tatgcgcatt cgtgaagagg tcggcaagta catccagcgt
     4261 ttacaagctt tagcccaagg aattgcgacg gttgatgtct tacagagtct ggcggttgtg
     4321 gctgaaaccc agcatttgat tcgacctgag tttggtgacg attcacaaat tgatatccgg
     4381 aaagggcgcc atgctgtcgt tgaaaaggtt atgggggctc agacctatat tccaaatacg
     4441 attcagatgg cagaagatac cagtattcaa ctgattacag ggccaaacat gagtgggaag
     4501 tctacctata tgcgtcagtt agccatgacg gcggttatgg cccagctggg ttcctatgtt
     4561 cctgctgaaa gcgcccattt accgattttt gatgcgattt ttacccgtat cggagcagca
     4621 gatgacttgg tttcgggtca gtcaaccttt atggtggaga tgatggaggc caataatgcc
     4681 atttcgcatg cgaccaagaa ctctctcatt ctctttgatg aattgggacg tggaactgca
     4741 acttatgacg ggatggctct tgctcagtcc atcatcgaat atatccatga gcacatcgga
     4801 gctaagaccc tctttgcgac ccactaccat gagttgacta gtctggagtc tagtttacaa
     4861 cacttggtca atgtccacgt ggcaactttg gagcaggatg ggcaggtcac cttccttcac
     4921 aagattgaac cgggaccagc tgataaatcc tacggtatcc atgttgccaa gattgctggc
     4981 ttgccagcag accttttagc aagggcggat aagattttga ctcagctaga gaatcaagga
     5041 acagagaatc ctcctcccat gagacaaact agtgctgtca ctgaacagat ttcactcttt
     5101 gatagggcag aagagcatcc tatcctagca gaattagcta aactggatgt gtataatatg
     5161 acacctatgc aggttatgaa tgtcttagta gagttaaaac agaaactata aaaccaagac
     5221 tcactagtta atctagctgt agcaaggaga cttctttgac aattctccac ttttttgcta
     5281 gaatgacatc acacaaacag aatgaaaagg agctgacgca ttgtcgctcc cttttgtcta
     5341 ttttttaagg agaaagtatg ctgattcaga aaataaaaac ctacaagtgg caggccctgg
     5401 cttcgctcct gatgacaggc ttgatggttg ctagttcact tctgcaaccg cgttatctgc
     5461 aggaagtctt aggcgccctc cttactggga aatatgaagc tatttatagt atcggggctt
     5521 ggttgattgg tgtggccgta gtcggtctag ttgctggtgg actcaatgtt gtcctcgcag
     5581 cctatattgc ccaaggagtt tcatccgacc ttcgggagga tgccttccgt aaaattcaaa
     5641 ccttttctta tgctgatatt gaacaattta atgcgggaaa tctagtcgtt cgaatgacaa
     5701 atgatatcaa ccagattcag aacgttgtca tgatgacctt ccagattctt ttcagacttc
     5761 ccctcttgtt catcggttcg tttatcctag cggttcaaac cttaccttct ctgtggtggg
     5821 tgattgttct catggtagtc ttgatttttg gtttgactgc tgtcatgatg ggaatgatgg
     5881 ggcctcgttt tgccaagttt caaacccttc ttgagcgcat caatgccatt gccaaggaaa
     5941 atttacgtgg cgttcgtgtg gtcaagtcct ttgtccaaga aaaagagcaa tttgctaagt
     6001 ttacagaggt ctcagacgag cttcttggtc aaaaccttta cattggttat gccttttcag
     6061 tagtggaacc ctttatgatg ttggttggtt acggggcggt cttcctctct atttggctgg
     6121 tcgcgggaat ggttcagtcg gatccgtctg ttgttggttc catcgcttct tttgttaatt
     6181 acctaagcca gattatcttt accattgtta tggttggatt tttgggaaat tctgtcagcc
     6241 gtgccatgat ttccatgcgt cgtattcgag aaattcttga cgcagagcca gctatgacct
     6301 tcaaggatgt cccagatgaa gagttggttg gaagccttag ctttgaaaat gtgaccttta
     6361 cctatccaat ggacaaggaa ccgatgctga aagatgtgag ctttactatt gaacctggtc
     6421 aaatggttgg tgtagttgga gcgactggtg caggaaagtc aaccttggct caattgattc
     6481 cacgtctctt tgatccacag gaaggggcta tcaaaatcgg aggtaaggat attcgagaag
     6541 tgagtgaagg aaccctgcgt aaaacagttt ccatcgttct ccaacgtgcc attcttttta
     6601 gtggaacgat tgcagataac ttgagacagg ggaagggaaa tgctactcta tttgaaatgg
     6661 agcgcgcagc caatattgcc caggctagtg aattcattca tcgtatggag aaaacctttg
     6721 aaagtccagt tgaagaacgg ggaaccaatt tctctggtgg acaaaaacaa aggatgtcga
     6781 ttgcgcgtgg gattgtcagc aatccacgta ttctgatttt tgatgattcg acctcagcct
     6841 tggatgccaa atcagagcgc ttggtgcaag aagctttgaa taaggacttg aaggggacga
     6901 caaccattat tattgctcaa aaaattagct cggttgtcca tgcagacaag atcttggttc
     6961 taaatcaagg acgattgatt ggtcaaggca cgcatgcaga cttggttgcc aacaatgccg
     7021 tttaccgtga aatctatgaa acacagaaa
//
LOCUS       DAVBGY010000080         7022 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVBGY010000080 DAVBGY010000000
VERSION     DAVBGY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.080"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(115..390)
                     /locus_tag="VQ620_001881"
     CDS             complement(115..390)
                     /locus_tag="VQ620_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET2764459.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(496..1335)
                     /locus_tag="VQ620_001882"
     CDS             complement(496..1335)
                     /locus_tag="VQ620_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2764460.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVIVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1477..2325
                     /locus_tag="VQ620_001883"
     CDS             1477..2325
                     /locus_tag="VQ620_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET2764461.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2418..3335)
                     /locus_tag="VQ620_001884"
     CDS             complement(2418..3335)
                     /locus_tag="VQ620_001884"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET2764462.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3772..3977
                     /locus_tag="VQ620_001885"
                     /pseudo
     CDS             3772..3977
                     /locus_tag="VQ620_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4288..4581)
                     /gene="rpmA"
                     /locus_tag="VQ620_001886"
     CDS             complement(4288..4581)
                     /gene="rpmA"
                     /locus_tag="VQ620_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET2764463.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4598..4942)
                     /locus_tag="VQ620_001887"
     CDS             complement(4598..4942)
                     /locus_tag="VQ620_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HET2764464.1"
                     /translation="MIQAVFERAEDGELRGAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(4958..5272)
                     /gene="rplU"
                     /locus_tag="VQ620_001888"
     CDS             complement(4958..5272)
                     /gene="rplU"
                     /locus_tag="VQ620_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET2764465.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYIEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5308..5395)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5490..6089)
                     /locus_tag="VQ620_001889"
     CDS             complement(5490..6089)
                     /locus_tag="VQ620_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HET2764466.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQAGNVVFLSVELIQNNVSDVR
                     DKLLTLITFIMGVFLLTIYKEKLRIVKKPILSLIPLAILSIIIGFVPQTVDNIYLVQP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGDVFSAYLTIFYHEKTILGVPIMMSIFYLSMLFA"
     gene            6559..6858
                     /locus_tag="VQ620_001890"
     CDS             6559..6858
                     /locus_tag="VQ620_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET2764467.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         2227 a         1414 c         1157 g         2224 t
ORIGIN      
        1 tacaatattt tcggaacatt caacttttta actctattta ttactagatt tcataattaa
       61 aaagcctact gaccaagcta gaaagcttga tacaataggc tttttaaaga ctgattattt
      121 aacagcgtct ttaagagctt taccagcttt gaatgctggt actttagaag ctgcaattgt
      181 catttcttta ccagtttgtg ggttgcgacc tttacgttct gcacgctcac gaacttcaaa
      241 gttaccaaaa ccgatcaatt gaactttttc accagctgca agatagtcag ctactgctgc
      301 aaatacagct tcaactgctg ctgctgagtc tttcttagtc aattctgtag cttctgctac
      361 tttagcgatc aaatcttgtt tgtttgccat gttaatgatt cctccagata atttctaatt
      421 aacaaatata atcatatcct aaaatccctt ttaggtcaag ttaaaaacgc tatttcttcc
      481 aattttattt attttttagg agtggtaacg tatcaaaata gcccaagcgt tctcacccgt
      541 gtgagtttga ataatggaac ccgtttccaa aacagaaatt ggcttttcaa cataagcttg
      601 taagctttct ttcatctctt ttgcccaatc atcactacca gaatatgaaa ttccaatctc
      661 tgctacagca cgttcagaaa gcgatgttat caactcatct aaccattttt taaatgtttt
      721 agttccacga cctttaacca ttggctgcaa ttcatggtct ttcatttgca tgacaacacg
      781 gatattgaga agggagctca acaagccagt tacacggcca attcgtccac ctttgacaag
      841 attttccaaa gttgaaacac caatataaag ctctgtatgg tttttaacct cttctacatg
      901 agataaaatt gcctccatat ctttaccttc ttgagctaac ttcgcagcct caacaacttg
      961 gaatttcagg gcttggtcag tgaaggaact atcaacaaca atcacatctg cagtagatag
     1021 gctagcacct tggcgtgctg cttctaccgt acccgaaaga gcatgggaca tatgaatagc
     1081 aagaatctgg ccaccatctt tgcataggtc ttcaaaaatc tcagcaaaga cacctacagg
     1141 tggctgactt gttttcggaa gattcttact ttcttgcatc aactgaagaa atttaccttc
     1201 ttctttcaaa tccgcatcag aataaacaac attatcaatc attacagata atggaacaat
     1261 tgtaatatct aattgcttta ctagttcagg ttcaatagta acagatgaat cggttacaat
     1321 cttaattttt gtcatagtat caatctttct attttaggat tcagattggt ttccttactt
     1381 ctaattatat caaaaaaaag attaaaaatc ctaatggagt caatcaaatt ttccgtaaaa
     1441 tttgatataa tcaacttata agaaaagagg tgtcctatga ttaaaaaaat ttaccccatt
     1501 tttaccattt tactaggtgc tgctatttat gcttttggac tgacttattt tgtagttccc
     1561 catcatctct ttgaaggagg ggcgacaggt attaccctca tcacatttta tctttttaaa
     1621 atccctgttt ccctcatgaa cctgctgatt aatattcccc ttttcatcct agcttggaag
     1681 atctttggag ccaaatccct ctattctagt ttactaggaa ccttagcttt gtccggctgg
     1741 ttagcttttt ttgagcatat tccccttcat attgatcttc aaggtgattt actaatcaca
     1801 gcccttatag cgggaatcct attgggaatt ggccttggaa ttatttttaa tgctggaggt
     1861 acaactggcg gaactgatat tctagctcgt attctcaaca aatacactca tatatccata
     1921 ggaaaactgc tctttatctt agatttttgt attctcatgt tgattctcct aatcttcaag
     1981 gatttgagat tggtttccta cacgcttttg tttgatttta ttgtttctcg tgttattgat
     2041 ttgattggtg aaggaggata tgccggcaaa ggctttatga ttatcacaaa acgtcctgac
     2101 caacttgcta aggcgattaa tgatgacctc ggaagaggtg ttacttttat ttctggtcaa
     2161 ggctactata gtaaagaaaa tttgaaaatc atctactgta ttgtcggaag aaatgaaatt
     2221 gtgaaaacga aggaaatgat tcatcgaatc gatcctcaag cctttataac tattacagaa
     2281 gcccatgaaa tcctaggaga aggcttcacc tttgaaaaag aataaaaaga ggtaatgtcg
     2341 tgacctcaaa agttagacta aatcatctat cttttgggtt acagacaacc tcttttttgt
     2401 tttatttact caagctctta agaccaattc cgagttactt cttcatcagc ctttaactga
     2461 tccactaatt ggtcaactga gtcaaatttg gtcatatcac gaatgcgatc aagccaataa
     2521 accatgacgg tttccccata aatatcttga ttaaaatcaa aaatattgac ttcaaaacgt
     2581 gcttcttctc catcaaaggt cacatttttc ccgacactag ccatagcacg atacttctgt
     2641 ctttgaatct caacatcaac gacataaacg ccatctgctg gcatataagt acggtctaaa
     2701 agcactaaat tcgctgtcgg ataaccaatt gtacgaccac gagcattacc atgaaccacc
     2761 atacctcttg atggaagcgg tgcccccaaa agttttcctg cttctttcac atttccatct
     2821 aaaatagctt gacggatacg agttgaacta atctttcctt tctcatcttc tacaggtgga
     2881 acaatgataa cttctccatc aaagtaatcc tttaaatctt ctgctgtttt tttgtcagaa
     2941 ccaaatgtat aatcaaaacc tgcaacaata attttggcat tcatagcctt gatataagtt
     3001 gcaaagaatt cttgtgcagt gagactagcg aattgactac taaaatcaag gagatataat
     3061 tcttctacac cttcgcgctt taattttctt tcacgttcag cagggttcaa aatatgcaaa
     3121 aacaaatccg gatgataagg ctctaaagcg atctttggag attcattaaa ggtcataacg
     3181 acgataggta ataaatcctt tctcgcagcc ttgttggcaa cacgaaataa ttcttgatgc
     3241 cccttatgta tgccatcaaa atagccgaga acaacgactg aatcagatgg tgtgccaata
     3301 tctttttggt tttttatagg aatagtaata atcataaaat aattatatca tagcgatagc
     3361 tatttctgga acagaaaatc tgaaatgttg tttttttcac atgaagtgta cctgttttca
     3421 aaaagcactt tattctatcg ttgcttaact atgaactttg caatattctt ctcaaaaact
     3481 tgtaggacat cttcaaaatt ttgcaaggag tgattagact tgttcggtaa ccataaagtg
     3541 tcatactatg ttatgtatga aaaagcaatg caactaactc ctgagaactt taaattacta
     3601 attggtgccg aaaaggtaga atttagaatc gaggtacacc tatggctgta aaatttacaa
     3661 aatgagacaa cttgggcaag atgtttgaag aatttcctaa actccctgat ttgaagcaag
     3721 tcactttccc tgatgacaaa gaaaaaagcc aaaacagcaa agaaaaacta gatgactgct
     3781 ttccaacaac tcccatctag tgtgcttcag actgggctat ttttctctcc atctgttagc
     3841 ttggattctc agaccgtttc agctaaagaa tatcttttcc cttatcagaa ggaacggctc
     3901 aaaccattca gacaagtgaa gggacgacaa gccaatattt gaaaccagat agcagttctt
     3961 atagtcaatt gaaataaaat ctgaagaaat cgagtaggaa actcatatca atttttaaca
     4021 gtgttctatt ccagattcat actcaatgaa aattaaagtg caaactagga agttagccgc
     4081 aggctgtact tgagtacggc aaggtgaaac tgacgtggtt tgaatttgat tttcgaagag
     4141 tatcaatcca ctatacatat taaaaagtat ggatatcaaa acaaaaaaac aggctctccg
     4201 aaaactcgga aagccttatt ttaagctgtt tctagcttcc tcacctttac atttcaaggt
     4261 tcgggataaa aggttcaatg gaccttttta tttagcgatt gggtaaacag aaacttgttt
     4321 tttatcgcgt cctttacgtt caaagcgtac tacgccttca actttagcga acaaagtatc
     4381 gtctccacca cgaccaacgt ttacacctgg atagatgtgt gtaccacgtt gacggtaaag
     4441 gattgatcca cctgttacag tttgtccgtc agctgcttta gctccaagac gttttgcttg
     4501 tgaatcacgt ccgtttgatg tagaacctcc accttttttg tgggcgaaaa gttgcaagtt
     4561 gttaagagtc atttttaaca taatgttttc ctccgtgtta gttttctgtg ataactctgg
     4621 tttggacgaa ctcagaatag ttctccgata agtttgccat acctaagaaa aatgattcaa
     4681 agaataactg ggtcatttct ctctggtgtg aaggaagatc ttttggtatt tcaaccatca
     4741 gatagccacc ttcatcttcg tttaattcta ggattggttc atagcctgca aatttctcaa
     4801 tagaattgat aaagttaatg gcaagcgtag aaaccgatgc acacacgaca tctaagccgt
     4861 attcgccact ctcggcgtgt ccagtaattt ccgcacccct cagctcgcca tcttcggctc
     4921 tctcaaagac tgcttgtatc atgtgttctc cttaaaatta agcgttaatt gcgttgatga
     4981 caacttttgt atatggttga cggtgacctt gtttacggtg gctacctttt ttaggtttgt
     5041 acttgtaagt aacaactttc ttttgttttc cttgtttttc aacagttcca actacagtag
     5101 ctccagcaac aagtggagtt ccgacaacag tgttttcacc accaacaaga acaacttcgt
     5161 taaaagtaac ttcttgacca gcttcaacgt tcaatttttc aatgtaaact gcttgaccaa
     5221 cttcaacttt aacttgtttt ccgccagttt tgataattgc gtatgtgctc attatgcacc
     5281 tcctatgatt tttatgggtt tccccgattt tttgtgaaga ctcgcctagc atcgtgggac
     5341 aaaccactta aacttgaata atcaagcaac gatgttcgtg cggttgcaca ggatcgtgca
     5401 tagtcaactc ttcaagtata gcatatctcc tattttctta caagtaataa cacctaaaat
     5461 caagcttttt cttttacttt tttctgccac taggcaaaaa gcatactgag ataaaaaatg
     5521 ctcatcataa taggaacacc aagaatggtc ttttcatgat agaaaatcgt caaataggct
     5581 gaaaagacat cgccaaggac aaaactacta agcaggctaa caaatatgaa cccttcacgc
     5641 aaaaaaggag tgtgcttagt tcggaaataa tctccaaaag ctagcattgt tcgtttgata
     5701 ttccctgtca taaaggcatt attgtaggca atacccgaaa cctcaccaaa agcagttgtc
     5761 accagtccca tacagaaggc caagggctgc actagataga tattatctac agtttgtggc
     5821 acaaaaccaa taattattga taggattgct aaaggaatca aggacagaat aggttttttc
     5881 acaattctca atttttcctt ataaatcgtt aataaaaaga ctcccattat aaacgttatt
     5941 aaggttaaaa gcttatccct aacatccgaa acattatttt gtattagctc taccgaaaga
     6001 aagacaacat ttccagcttg tccagctaca agggtattcc ctcgaacaat aaaagtgtaa
     6061 gcatccacat atccagcaca aaatgtcaaa aaagtgctaa ccttttagac tgacgtgata
     6121 tttttcttat aggtaataac ctcattttcc ctcccattgt attttctctt agaaatattg
     6181 taccatttct ttctaaaaaa tcgtagacta ccatttagat tttactatta gcataaaaat
     6241 aataatagac aactatttat ccaaaaatag atagaggtaa catgtttgca aacaaagcat
     6301 acgaaccttt agtaaaatca tttccatgaa actagaatag agcccctctt agcaaaaatc
     6361 attattttaa tttatttcta atcactcctt gacataaata actctcacca ataaaagact
     6421 atgtcttaaa aaaatggtat aataaaatca atacctgggc ttgatggcta tgctactaat
     6481 aacaattagg agagaaaatc aggcacttgt taacaacaag gattatcccc ttgagatgaa
     6541 aggaacttta gaaatcttat gatgaacatg caaaacatga tgcgtcaagc acaaaaactt
     6601 caaaaacaaa tggaacaaag ccaagctgaa cttgctgcta tgcaatttgt tggcaaatct
     6661 gctcaagatc ttgtccaagc gaccttaact ggcgataaga aagttgtcag cattgatttc
     6721 aatccagctg tcgttgaccc agaggacctt gagactcttt ctgatatgac cgttcaagcc
     6781 atcaactctg ctcttgaaca aatcgatgaa actactaaga aaaaactggg tgctttcgct
     6841 gggaaattgc cattctaaaa acaaggagct agaacaatac ttgtcgataa caaaggctaa
     6901 gaaaggtgca aaaattataa aaaacgcata gtatcaagca acctaaaact ttgggctcta
     6961 taatatttgt agtgggtaaa tccactatag atattatgga gcctatttta ttgtagaaaa
     7021 aa
//
LOCUS       DAVBGY010000081         6638 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVBGY010000081 DAVBGY010000000
VERSION     DAVBGY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6638
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.081"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..154
                     /locus_tag="VQ620_001891"
     CDS             <1..154
                     /locus_tag="VQ620_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2764468.1"
                     /translation="FGHKLLPLPPYSPEYNPIEKTWVHIKKHLKKVLPSCNTFYEALL
                     SCSCFN"
     gene            215..1177
                     /locus_tag="VQ620_001892"
     CDS             215..1177
                     /locus_tag="VQ620_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET2764469.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSSNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVIIFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVEPLRYE"
     gene            1354..2361
                     /gene="gap"
                     /locus_tag="VQ620_001893"
     CDS             1354..2361
                     /gene="gap"
                     /locus_tag="VQ620_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET2764470.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2428..3264)
                     /locus_tag="VQ620_001894"
     CDS             complement(2428..3264)
                     /locus_tag="VQ620_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2764471.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQYLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETYVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3390..5585
                     /locus_tag="VQ620_001895"
     CDS             3390..5585
                     /locus_tag="VQ620_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET2764472.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMASQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVAAVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIAYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5638..5790
                     /gene="rpmG"
                     /locus_tag="VQ620_001896"
     CDS             5638..5790
                     /gene="rpmG"
                     /locus_tag="VQ620_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2764473.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5800..5976
                     /gene="secE"
                     /locus_tag="VQ620_001897"
     CDS             5800..5976
                     /gene="secE"
                     /locus_tag="VQ620_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET2764474.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6032..6568
                     /gene="nusG"
                     /locus_tag="VQ620_001898"
     CDS             6032..6568
                     /gene="nusG"
                     /locus_tag="VQ620_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET2764475.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2058 a         1164 c         1441 g         1975 t
ORIGIN      
        1 gtttgggcat aaacttttac ctcttcctcc ctactcacct gagtacaatc ctattgagaa
       61 aacatgggtt catatcaaaa agcacctcaa aaaggtatta ccaagttgca atacctttta
      121 cgaggctctt ttgtcttgtt cttgtttcaa ttgactatat atcacggata cagttgggaa
      181 agaagctatt aaacatttgg agagtccttt tgggatgaac atgtcaaata ttactataga
      241 tgggcaagaa atggatctga gtcaaacgat gccttcttat gctcaagtag tgagtcttta
      301 tgatgataca agtatctctg ttagtagtaa tgagacggac aaagtgttgg ctggctccct
      361 ctatactgat acaaatgaac agggattaac gattccaagc agtttactaa aaaattggaa
      421 tgaacagaca ggaaaaaatt tgacagctaa tgatcttatt ggcaaatcag tctcagccag
      481 cattgtagaa agtgctgccg aaactagtaa gattgctcaa tttcaaacga agattgtacg
      541 tgtaatcaat gatgaagatg acatggagga cagcaacagt ttcatgctgt ctcatcaaat
      601 ggaaacgatt ttgaaagagg ctggatttac gaaagctgta tcttatttta tcttggaact
      661 caaagatcca tcacagacaa aagtagtaac agaagaatta cagaaaaata agaagtatac
      721 tgtgctttct caacagagag ttcttgatat tgtgattatc tttattcgtg ttattcaggg
      781 attattgatt gtgctttcat cacaagctat tgtggtagca gcggttatga ttggtatcat
      841 tatttacatc aatatcatgc agcgttccaa ggaaataggt gtcatgaagg cagttggtta
      901 tcagaatcgt gatgtcaaag gaatttttat ttacgaggct atctggattg taggcatcgc
      961 cttgctgctg gcatttttgg ttgcacaagg ggtgggaagt ttggcgaatg cgattgtaag
     1021 tcacttttac ccatccatca ctaaagtttt tgaattaaat cttttatctg ttttaggaac
     1081 tctagttttc gctctattac ttggttatgt ctcagcctac ttcccggcgc gtaagattag
     1141 taaaatggat cctgtagaac cgttacgcta tgaatagtaa gggattcctt gatttacagt
     1201 cttaaaaaga aactaacata gaaaaacaat ggacatactt gcaattcaac tcaaatagtt
     1261 gtataatagg tttgttgaaa ggtgatttgt agtgattcaa gttactcttt tcacaataaa
     1321 aatttcatga ttttcataag gaggaaatca ctaatggtag ttaaagttgg tattaacggt
     1381 ttcggacgta tcggtcgtct tgctttccgt cgtatccaaa acgtagaagg tgttgaagtt
     1441 acacgcatca acgaccttac agatccagtt atgcttgcac acttgttgaa atacgacaca
     1501 actcaaggtc gtttcgacgg tactgttgaa gttaaagaag gtggatttga agttaacggt
     1561 aaattcatca aagtttctgc tgaacgtgat ccagaacaaa tcgactgggc tactgacggt
     1621 gtagaaatcg ttcttgaagc tactggtttc tttgctaaga aagaagcagc tgaaaaacac
     1681 cttaaaggtg gagctaaaaa agttgttatc actgctcctg gtggaaacga cgttaaaaca
     1741 gttgtattca atactaacca cgacgttctt gacggtactg aaacagttat ctcaggtgct
     1801 tcatgtacta caaactgctt ggctccaatg gctaaagctc ttcaagacaa ctttggtgtt
     1861 gttgaaggat tgatgactac tatccacgct tacactggtg accaaatgat ccttgacgga
     1921 ccacaccgtg gtggtgacct tcgccgtgct cgcgctggtg ctgcaaacat cgttcctaac
     1981 tcaactggtg ctgcaaaagc tatcggtctt gtaatcccag aattgaatgg taaacttgat
     2041 ggatctgcac aacgcgttcc aactccaact ggatcagtta ctgaattggt agcagttctt
     2101 gaaaagaacg ttactgttga tgaagtgaac gcagctatga aagcagcttc aaacgaatca
     2161 tacggttaca cagaagatcc aatcgtatct tcagatatcg taggtatgtc ttacggttca
     2221 ttgtttgacg caactcaaac taaagttctt gacgttgacg gtaaacaatt ggttaaagtt
     2281 gtatcatggt acgacaacga aatgtcatac actgcacaac ttgttcgtac tcttgaatac
     2341 ttcgcaaaaa ttgctaaata attcttgagt tgatagaaag caaggctttg tggtcttgct
     2401 ttttatatgg aaaaatggat gacacgatca tccattcttt tttaattctt tttcaaatgt
     2461 atttgaaagg gtagtgaaag ttagcttctc taaagtaagt gggtgggtaa aggaaagtcg
     2521 gaaggcatga agcataagcc gacttgtctt tgatttacta ttatagagag ggtcgcccag
     2581 gataggaaga ttatgatgcg aaaggtgcac acgaatctga tgggttcgcc ctgtctttag
     2641 cttgcaacga gccaaggaag tcttgtttga gaattgcttt aatctgctta catacgtttc
     2701 agcatattgc ccattttttg catcaactat tctttttcta cgatcatggc gatcacgtcc
     2761 aattttgtct ctgaaaacaa gttcttttct gttgatattt ccatcaacta gagcccaata
     2821 ttctctagaa atctcttttt tctccaataa gcgattgaga atgggcagga taaaaggatt
     2881 tttggcaaag agaactaagc cactggtttc catgtccaga cgatgaacga catagcaggt
     2941 ttggccaaca taggtactga catggttaag aagggcaatt tcgtttggtt gattaccatg
     3001 cgttttcatc ccctctggtt tgtttacaat aatcaagtat tgatcttgat aaacttcctg
     3061 cactaagtct gggttgcccc aagggatcgt cttttgggga taatcttcct cgtcaaaagt
     3121 caactggcaa acatctccag gatttacgat ttcgttccag tggacttctt cttgatttat
     3181 caaaatatgt ttcttgattc tcaaaaaatg acggattttt ctagggatga ggagttgttc
     3241 ctcaagtaat tgctttaccg tcatttgagg tagagaggcg ggtaatgtaa atgtgaattg
     3301 catacagata ttgtaacaaa aaaagcccta tttggatagg aaatagctaa attcttgtct
     3361 tcctatgatg aagatgataa aataaacgca tgaaattaga taaattattt gagaaatttc
     3421 tttctctttt taaaaaagaa acaagtgaac tagaggactc tgattctact atcttacgtc
     3481 gctctcgtag tgatcgaaaa aaattagccc aagtaggtcc gattcgaaaa ttctggcgtc
     3541 gttatcatct aacaaagatt atccttatac taggtttgag tgcaggcttg ctagttggaa
     3601 tctatttgtt tgctgtagcc aagtcgacca atgtcaatga tttgcaaaat gccttgaaaa
     3661 ctcggactct tatttttgac cgtgaagaaa aagaggctgg tgccttgtct ggtcaaaagg
     3721 gaacctatgt tgagctgact gatatcagta aaaacttgca gaatgctgtt attgcgacag
     3781 aagaccgttc tttctataaa aatgacggga ttaactatgg ccgtttcttc ttggctattg
     3841 tcactgctgg acgttcaggt ggtggctcta ccattaccca acagctggct aaaaacgcct
     3901 atttatcaca ggatcaaact gttgagagaa aagcgaaaga atttttcctt gccttagaat
     3961 taagcaaaaa atatagtaag gagcaaattc taaccatgta ccttaacaac gcttattttg
     4021 gaaatggtgt gtggggtgta gaagatgcga gtaagaaata ctttggagtt tctgcatcag
     4081 aagtgagtct ggatcaagct gcgactctgg cagggatgct caaggggccg gaactgtata
     4141 atcccttgaa ttccgtagaa gattctacta atcggcgcga tactgtcttg cagaatatgg
     4201 ttgcagcagg atatattgat aaaaaccaag aaaccgaagc tgctgaagtt gatatggctt
     4261 cgcaattgca cgataagtat gaaggaaaaa tctcagatta ccgttacccc tcttattttg
     4321 atgcggtggt taatgaagct gtttccaagt ataatctaac agaggaagag attgtcaata
     4381 atggctaccg catttacaca gagctggacc aaaactacca agcaaatatg cagattgttt
     4441 atgaaaacac atcgctattt ccgagggcag aggatggaac gtttgctcaa tcaggaagtg
     4501 tagctctcga accgaaaaca gggggagttc gtggagttgt cggtcaagtt gctgacaatg
     4561 ataaaactgg attccggaat ttcaactatg caacccaatc aaagcgtagt cctggttcta
     4621 caattaagcc tttagttgtt tatacgccag cagttgaagc aggctgggct ttgaataagc
     4681 agttggataa ccataccatg cagtatgaca gctataaggt tgataactat gcagggatca
     4741 aaacgagtcg agaagttcct atgtatcaag ccttggcaga atcgcttaat ctacctgctg
     4801 ttgccgctgt taatgatttg ggtgtcgaca aggcttttga ggcaggcgaa aaattcggac
     4861 tcaacatgga aaaggtcgac cgtgttcttg gtgtcgcctt gggaagcggt gttgaaacca
     4921 accctcttca aatggctcaa gcatacgctg cctttgcaaa tgaaggttta atgcctgaag
     4981 ctcattttat tagtagaatt gaaaatgcta gtggacaagt tattgcgagt cataaaaatt
     5041 cacaaaaacg ggtgattgat aagtctgtag ctgacaagat gaccagtatg atgttgggga
     5101 ctttcaccaa cggtaccggt attagttcat cgcctgcaga ctatattatg gcagggaaaa
     5161 ctggaacaac tgaagcagtt ttcaatccgg agtacacaag tgaccagtgg gtaattgctt
     5221 atactccgga tgtagtgatt agccactggc ttggctttcc taccactgat gaaaatcact
     5281 atctagctgg ctctacttca aacggtgcag ctcatgtctt tagaaacatt gccaatacca
     5341 ttttacctta tacgccagga agtaccttta cggttgaaaa tgcttataag caaaatggaa
     5401 ttgcaccagc caatacaaaa agacaagtac aaaccaatga taatagccag acagatgata
     5461 atttgtctga tattcgaggg cgtgcgcaaa gtctagtaga tgaggctagc cgggctatct
     5521 cagatgcgaa gattaaggaa aaggctcaaa caatatggga ttcgatagtc aatctatttc
     5581 gctaagatgc ttgtcaaagc ctagctttct tgttataatg gataggatgg aggcgttatg
     5641 gcactaaaaa aagcaagcct agcttgtgcg gtttgtggtt cgagaaacta ttcaatcaag
     5701 atcagcggaa accccaagcc tacacgacta gaagtaaata aattttgtaa gcattgtggc
     5761 aagtacacta cacacagaga aacgagatag gagagagcga tgcgttttat tggagatatt
     5821 tttagacttc ttaaagacac aacgtggcca actcgcaagg aaagctggag agattttcgt
     5881 tctatcatgg aatacacagc tttctttgta gtaattattt acatttttga ccagttgatt
     5941 gtttcaggtt tgattcgatt tattaacatt ttttagaaga ttagtggagt taattacact
     6001 agaaatcttc tatattatga aaggaaatat catggatagt tttgataaag ggtggtttgt
     6061 tttacaaact tattctggtt atgaaaataa ggtgaaagaa aatctattac aacgtgcaca
     6121 aacctacaat atgttggata atattctacg cgttgaaatt ccaacacaaa cagtgcaagt
     6181 tgaaaaaaat ggaaagagaa aagaagtaga agaaaatcgc tttccaggtt atgttcttgt
     6241 agaaatggtc atgacagatg aagcttggtt tgttgttcga aacacaccaa atgttacagg
     6301 atttgtcgga tcacacggga acagatcaaa accaactcca ttattggaac aagaaattcg
     6361 tgacattttg gtatctatgg gacaaactgt tcaagaattt gatttcgatg ttgagattgg
     6421 tcaaactgta cgtattattg atggtgcttt tgcagactac actggtaaga ttacagaaat
     6481 tgataataac aaagtgaaaa tgattatctc tatgtttggt aatgacacag ttgcagaagt
     6541 aaacctaaac caaatcgcag aattataacc ctgagagagg ctggagcctc tcttttttgt
     6601 gcagtttagg agctaaaggg aacagaatgg agaaaatg
//
LOCUS       DAVBGY010000082         6525 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVBGY010000082 DAVBGY010000000
VERSION     DAVBGY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.082"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            222..422
                     /locus_tag="VQ620_001899"
     CDS             222..422
                     /locus_tag="VQ620_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764476.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            447..863
                     /locus_tag="VQ620_001900"
     CDS             447..863
                     /locus_tag="VQ620_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764477.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1094..1801
                     /gene="ccdA2"
                     /locus_tag="VQ620_001901"
     CDS             1094..1801
                     /gene="ccdA2"
                     /locus_tag="VQ620_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET2764478.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1812..2369
                     /gene="sdbB"
                     /locus_tag="VQ620_001902"
     CDS             1812..2369
                     /gene="sdbB"
                     /locus_tag="VQ620_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HET2764479.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2490..3881
                     /locus_tag="VQ620_001903"
     CDS             2490..3881
                     /locus_tag="VQ620_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET2764480.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4024..4941
                     /gene="adcAII"
                     /locus_tag="VQ620_001904"
     CDS             4024..4941
                     /gene="adcAII"
                     /locus_tag="VQ620_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HET2764481.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            4949..>6525
                     /locus_tag="VQ620_001905"
     CDS             4949..>6525
                     /locus_tag="VQ620_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2764482.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNHFHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLVFDPAQITYRTVNGVAVPHGDHYHFIPYSQLSPLEEKLA
                     RMIAVKGQNGAVLPGMHYLKPAPKPQVQPSTEKKQTDFAVEQVVRKVGEGYVVEIAGV
                     SHYVFAKDLAKDKIDAIENLLSKKTQETHALVAKKENVAPRDQEFYDKAYNLLTQAHK
                     VLSENKGRTSDFQALDKLAERLNNESSNKVKLVDDLLAFLAPITHPERLGKPNAQITY
                     TDDEIQVAKLAGKYTTE"
BASE COUNT         2012 a         1188 c         1371 g         1954 t
ORIGIN      
        1 atggtttcag aaatttctct taccaacatt aaccacacca tcggttatta tagtaaaatg
       61 gaataagaac aggacaaatc gatcaggaca gtaaaatcga attctaacaa tgttttagaa
      121 gtctatatgt actattctag tttcaatata ccataactgc tcctagtcaa cctgcaagtc
      181 ctgtcccagc tccaagctca gctccgactc aagatcctag agtggtagca agcgcttcgg
      241 ctagctcaac gagcacacaa gctcaagagc aagttgacaa gtctgaactt cgtgccttga
      301 gtcaagagtt agaccaacgc ttgaaagctt tggcaacagt atctgatcca aaaatcgatg
      361 caaccaaggc tgtcctccta gatgctcaaa aagctccgga agatagtgcc ttgacagagt
      421 aaggtctgca tactgcagta gagtctgtga aggctgcctt ggactctctc aaagatgtga
      481 aagcaaatgc tagcgacagc aagcctgcac aggacaagaa ggatgcaaaa caaggaacgg
      541 aagatagtaa ggattcagat aagatgactg aaacaaactc agttccggca ggagtgattg
      601 tggtcagtct acttgccctc ctaggcgtga ttgccttctg gctgattcgc cgtaagaaag
      661 agtcagaaat ccagcaatta agcacggaat tgatcaaggt tctaggacag ctagatgcag
      721 aaaaagcgga taaaaaagtc cttgccaaag cccaaaacct tctccaagaa acccttgatt
      781 tcgtgaaaga agaaaatggc tcagcagaga cagaaactaa actagtagag gagcttaaag
      841 caatccttga caaactcaag taaaacatga tttatgaaat gtcagaccta aaagacattt
      901 tcgttcacaa aagcagtgac gagtaatcct tgtcgttgct ttttggctat tttgggggat
      961 aagatccgcc ttgactagca aaagaaaaga ttggtccaat agtgcccgat acgattttgc
     1021 gtagcagaaa gcttacaaat aaaattgaat atagtaaaat aggattagaa ttattaagaa
     1081 agttggttct ttattggaaa cgatagtatt tttaatctct gtttttctag caggtgtttt
     1141 atcctttttt tctccttgta tttttcctct tctgccagtc tatgctggga ttttattgga
     1201 tgatcaagaa agtgcaaaaa gcttttcttt gtttgggaga aaggttctct ggtcaggctt
     1261 gattcgaaca ctttgcttta tcgctggtat ctctctcatt ttctttattc taggctttgg
     1321 tgctggttac tttggtcata ttctctatgc aaattggttt cgatatgtca tgggagctat
     1381 tattatcatt ttgggtcttc accagatgga aatttttcat ttgaagaaat tagaagttca
     1441 aaaaagtttt acctttaaaa aatcagattc taatcgttat tggtcagctt ttttacttgg
     1501 tattaccttt agctttggtt ggacaccttg tattggtcca gttttaagtt ctgttttagc
     1561 acttgcggct tctggaggca atggtgcttg gcaaggagcg atttatactc tcatttacac
     1621 tctgggcatg gcccttcctt tcttggtctt ggcactagct tcaggtctag tcatgccata
     1681 ttttagtaaa atcaagcgtc atatgatgct actaaagaaa attggtggtt tcctcattgt
     1741 tttaatggga attttgttac tattaggaca agtaaatgtt ctagctggaa tttttgaata
     1801 aaggaggaca aatgaaaaaa gtaatgtttg ctggcttaag tctcttgtca ttagttgtat
     1861 tgatggcctg tggtgaggaa gaaactaaaa agactcaagc agcacaacag ccaaaacaac
     1921 aaacgactgt acaacaaatt gctgttggaa aagatgctcc agacttcaca ttgcaatcca
     1981 tggatggcaa agaagttaag ttatctgatt ttaagggtaa aaaggtttac ttgaagtttt
     2041 gggcttcatg gtgtggtcca tgcaagaaaa gtatgccaga gttgatggaa ctagcggcga
     2101 aaccagatcg tgatttcgaa attcttactg tcattgcacc aggaattcaa ggtgaaaaaa
     2161 ctgttgagca attcccacaa tggttccagg aacaaggata taaggatatc ccagttcttt
     2221 atgataccaa agcaaccacc ttccaagctt atcaaattcg aagcattcct acagaatatt
     2281 taattgatag ccaaggaaag attggaaaga ttcaatttgg tgctatcagt aatgcggatg
     2341 cagaagcagc atttaaagaa atgaactaga atcgataaaa tctgattaca agatgtagtc
     2401 agattttttt agaaaaacat tttataaata ttcacatatc tcctatattt atggtaaaat
     2461 agaattatca gtttattttg gagtcaaaga tgaatatatt tagaacaaag aatgttagtt
     2521 tagataaaac agagatgcat aggcatttga agttatggga tttgattttg ctgggtatcg
     2581 gagccatggt agggacaggc gtctttacaa tcacaggtac tgcagctgca acacttgctg
     2641 gcccagccct agtgatttca atcgttattt ctgccttgtg tgtgggatta tcagctctct
     2701 tttttgcaga atttgcctcg cgagtacccg ctacaggagg tgcctatagt tacctctatg
     2761 ctatcttagg agaattccct gcctggttgg ctggttggtt aaccatgatg gagttcatga
     2821 cagccatatc aggcgtagct tcgggttggg cagcttattt taaaggcttg ctctctcaat
     2881 acgggatagc ctttcctcag gctttaaatg gtacctttaa tcctcaagca gggacatttg
     2941 ttgatttatt gcctattctt gtcttggtct tggtgacttc gcttgtttta ctaaatgcca
     3001 aggcagcctt acgctttaat tcgattctag ttattttgaa attttccgct ttagctctct
     3061 ttgttttagt aggaatttgg aatattaagt ttgataattg gagcaatttt gctccttacg
     3121 gttttggaca aatctatgga gctagcactg gtattatggc tggtgcgtcc ttgatgttct
     3181 tcggtttttt gggatttgaa tccatctcta tggctgtaga tgaagtcaag actcctcaaa
     3241 aaaatattcc tagagggatt gtcttatcgc tttctatcgt aaccattctc tatgccttgg
     3301 tgacccttgt tttgactggt gtggttcact atagtcattt aaatgtcgat gatgccgttg
     3361 cctttgccct tcgtagtgtt gggattagtt gggcagccaa ctatgtgtca ttagtggcta
     3421 tcttgacctt gattacagtt tgtatttcga tgacctatgc cctatcgcgt atgatttaca
     3481 gtttagcacg tgacggctta gtgcctgctg cctttaagga actgacgaag actagcaaga
     3541 taccaaagaa tgctactatc ttgacaggtc tagcttcagc agtagcagca ggaatgttcc
     3601 cactagccag tattgcagcc tttttaaata tttgtacctt agcctacttg atcatgctgg
     3661 cttatggtct gattcgctta cggaaagaaa aaggaatgcc caaagctggg gaatttaaaa
     3721 caccattggt acctctgtta ccaattttat cgatcatcat ttgtttatcc ttcatgttac
     3781 agtataatat gaacacctgg attgctttcc tagttgcgtt gttagtagga agtattattt
     3841 acttcactta tggctataag cattctacca tagaagaata aagtgaaagt ctattgagtt
     3901 gttgacactc agtagatttt tatatgtgaa agaaatttca aattttttct aaaaatatct
     3961 tgacagatta aattttcagg agtagaatat ttactagtta attaaatggt taaggagttg
     4021 ttcatgaaga aacaaaattt atttttagtc ctgttaagtg tctttctttt gtgtttgggg
     4081 gcttgtggtc aaaaggaaag tcagacagga aaggggatga aaattgtgac cagtttttat
     4141 cctatctacg ctatggttaa ggaagtatct ggtgacttga atgatgttcg gatgattcag
     4201 tcaagtagtg ggattcactc ctttgaacct tcggcaaatg atatcgcagc catctatgat
     4261 gcagatgtct ttgtttacca ttctcataca ctcgaatctt gggcaggaag tctggatcca
     4321 aatctaaaaa aatccaaagt gaaggtctta gaggcttctg agggaatgac cttggaacgt
     4381 gtccctggac tagaggatgt ggaagcaggg gatggagttg atgaaaaaac gctctatgac
     4441 cctcacacat ggctagatcc tgaaaaagct ggagaagaag cccaaattat cgctgataaa
     4501 ctttcagagg tggatagtga gcataaagag acttatcaaa aaaatgcgca agcctttatc
     4561 aaaaaagctc aggaattgac taagaaattc caaccaaaat ttgaaaaagc gactcagaaa
     4621 acatttgtaa cacaacatac agccttttct tatctagcga agagatttgg gcttaatcaa
     4681 cttggtattg caggtatctc tcctgaacaa gaaccaagtc cacgacaact aacagaaatt
     4741 caggaatttg ttaagaccta taaggttaaa acgattttta cagaaagtaa cgcttcttca
     4801 aaagtagctg aaactcttgt caaatcaaca ggtgtgggtc ttaaaactct gaatccttta
     4861 gagtcagacc cacaaaatga caagacctat ttagaaaatc ttgaagaaaa tatgagtatt
     4921 ctagcagaag aattaaagtg aggaaagaat gaaaatcaat aaaaaatatc tagcaggttc
     4981 agtggcagtc cttgccctaa gtgtttgttc ctatgaactt ggtcgtcacc aagctggtca
     5041 ggttaagaaa gagtctaatc gagtttctta tatagatggt gatcaggctg gtcaaaaggc
     5101 agaaaatttg acaccagatg aagtcagtaa gagagagggg atcaacgccg aacaaattgt
     5161 tatcaagatt acggatcaag gttatgtgac ctctcatgga gaccattatc attactataa
     5221 tggcaaggtt ccttatgatg ccatcatcag tgaagaactt ctcatgaaag atccgaatta
     5281 tcagttgaag gattcagaca ttgtcaatga aatcaagggt ggctatgtga ttaaggtaga
     5341 cggaaaatac tatgtttacc ttaaagatgc agcccatgcg gacaatattc ggacaaaaga
     5401 agagattaaa cgtcagaagc aggaacgcag tcataatcac aactcaagag cagataatgc
     5461 tgttgctgca gccagagccc aaggacgtta tacaacggat gatgggtata tcttcaatgc
     5521 atctgatatc attgaggaca cgggtgatgc ttatattgtc cctcacggca atcacttcca
     5581 ctatattcct aagagtgatt tgtctgccag tgaattagct gctgcccaag cctattggaa
     5641 tgggaagcag ggatctcgtc cttcttcaag ttctagtcat aatgcaaatc cagctcaacc
     5701 aagattgtca gagaaccaca atctgactgt cactccaact tatcatcaaa atcaagggga
     5761 aaacatttca agccttttac gtgaattgta tgctaaaccc ttatcagaac gccatgtaga
     5821 atctgatggc ctagtatttg atccggcaca aataacatat cgtacagtca atggtgttgc
     5881 tgtaccgcac ggagaccatt atcactttat tccttattca caactgtcac ctttggaaga
     5941 aaaattggct cgtatgattg ctgttaaggg acaaaatggt gcagtcttgc caggaatgca
     6001 ttatttgaaa ccagcaccaa aaccgcaagt tcaaccatca actgagaaaa aacaaactga
     6061 ttttgcagta gaacaggttg ttagaaaagt tggagaaggc tatgttgtgg aaattgctgg
     6121 tgtcagtcac tatgtttttg ctaaagactt ggctaaggat aagattgatg ctattgaaaa
     6181 tctcttgtct aagaaaactc aagagacaca tgctctagtt gctaaaaaag aaaatgtcgc
     6241 tcctcgtgac caagaatttt atgataaagc ttacaatctg ttaacccagg ctcataaagt
     6301 cttgtctgaa aataaaggtc gtacttctga tttccaagcc ttagacaaat tagcagaacg
     6361 cttgaataat gaatcttcta ataaagtaaa attggtagat gatttattgg cattcctagc
     6421 accaattacc cacccagaac gccttggcaa accaaatgcg caaattacct acactgatga
     6481 tgagattcaa gtagccaagt tggcaggcaa gtacacaaca gaaga
//
LOCUS       DAVBGY010000083         6489 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVBGY010000083 DAVBGY010000000
VERSION     DAVBGY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.083"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..211
                     /locus_tag="VQ620_001906"
     CDS             <1..211
                     /locus_tag="VQ620_001906"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764483.1"
                     /translation="PNPSNRRDDRVELSHQVIDTFFRLVNEPLVMFDDFITRDKTSMR
                     YQTHYQLKQKAVVRSFRKSSSDWRK"
     gene            298..2064
                     /gene="patB"
                     /locus_tag="VQ620_001907"
     CDS             298..2064
                     /gene="patB"
                     /locus_tag="VQ620_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HET2764484.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTHVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            2203..2892
                     /locus_tag="VQ620_001908"
     CDS             2203..2892
                     /locus_tag="VQ620_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET2764485.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLDEL"
     gene            2896..3075
                     /locus_tag="VQ620_001909"
     CDS             2896..3075
                     /locus_tag="VQ620_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764486.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            3062..4411
                     /locus_tag="VQ620_001910"
     CDS             3062..4411
                     /locus_tag="VQ620_001910"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET2764487.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            4535..5995
                     /locus_tag="VQ620_001911"
     CDS             4535..5995
                     /locus_tag="VQ620_001911"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET2764488.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            6009..6443
                     /locus_tag="VQ620_001912"
     CDS             6009..6443
                     /locus_tag="VQ620_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2764489.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNTYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT         2031 a         1250 c         1396 g         1812 t
ORIGIN      
        1 tcccaatccc agtaaccgtc gtgatgaccg agttgaactc agccatcaag tcattgacac
       61 atttttccgc cttgtcaatg agcctcttgt aatgtttgat gatttcatta cacgagataa
      121 aacgtctatg cgttatcaaa ctcattacca attaaaacaa aaagctgtgg ttagatcctt
      181 tcggaaatcg tcaagcgatt ggaggaaatg aactaatcca cagcggctta ttccaagtat
      241 accacttggg ctttggcagt agctaactgc gctaaatata atataaggag gagtaaaatg
      301 aagacagttc aatttttttg gcattatttt aaggtctaca agttctcatt tgtagttgtc
      361 atcctgatga ttgttctggc gacttttgcc caagccctct ttccagtctt ttctggacaa
      421 gcggtgacgc agctagccaa tttagttcaa gcttatcaaa atggcaatcc agaacttgta
      481 tggcaaagcc tatcaggaat catggtcaat cttggcctgc tggttttggt tctatttatc
      541 tctagtgtaa tatacatgtg tctcatgacg cacgtgattg cagaatcgac caacgagatg
      601 cgcaaaggcc tctttggtaa gcttgctcag ttgacggttt ctttctttga ccgtcgacaa
      661 gatggcgata tcttgtctca ttttaccagt gatttggata atatcctcca agcctttaac
      721 gaaagcttga ttcaggtcat gagcaatatt gttttataca ttggtctgat tcttgtcatg
      781 ttttcgagaa atgtgacgct ggctctcatc accattgcca gcaccccatt ggctttcctt
      841 atgctgattt tcatcgtgaa aatggcacgc aaatacacca acctccagca gaaagaggta
      901 gggaagctca acgcctatat ggatgagagc atctcaggcc aaaaagccgt gattgtgcaa
      961 ggaattcaag aggatatgat ggcaggattt cttgaacaaa atgagcgcgt gcgcaaggca
     1021 acctttaaag gaagaatgtt ctcaggaatt cttttccctg tcatgaatgg gatgagcctg
     1081 attaatacag ccatcgtcat ctttgctggt tcggctgtac ttttgaatga taagtctatt
     1141 gaaacaagta cagccctagg tttgattgtt atgtttgcac aattttcaca gcagtactac
     1201 cagcctatta tccaagttgc agcgagttgg ggaagccttc agttggcctt tactggagct
     1261 gaacgaattc aggaaatgtt tgatgcagag gaggaaatcc gacctgaaaa ggctccaacc
     1321 ttcactaagt tgcaagaaag tgttgaaatc agtcatatcg atttttcata cttgcctgat
     1381 aaacctattt tgaaagatgt cagcatttct gcccctaaag gccagatgac agcagttgtt
     1441 gggccgacag gttcaggaaa aacgactatt atgaacctca tcaatcgctt ttatgatgtt
     1501 gatgctggtg gtatttattt tgatggtaaa gacattcgtg gctatgactt agatagtctt
     1561 agaagcaagg tgggaattgt attgcaagat tcggtcttgt ttagcggaac gattcgagac
     1621 aatatccgat ttggtgtgcc agatgctagt caggaaatgg ttgaggtagc agcaaaagca
     1681 acccacattc acgactatat cgaaagtttg cctgataagt acgatactct tattgatgat
     1741 gaccagagca tcttttcaac agggcagaag caattgattt caatcgctcg aaccctgatg
     1801 acagatccag aagttctcat tctcgatgaa gcaacttcaa acgtagatac ggtgacagaa
     1861 agcaagattc agcatgccat ggaggtggtt gtagcaggta gaactagttt cgtcattgcc
     1921 caccgcttga aaaccattct caatgcagat cagattattg tccttaaaga tggagaagtc
     1981 attgaacgtg gtaaccacca tgaacttttg aagctaggtg gcttttattc agaactctat
     2041 cacaatcaat ttgttttcga ataagaaaga agttgtccta tgtgggcagc tttttcttgt
     2101 ccataaaaaa tgtttatcac agccttaaaa aaaacatatt agacgaaagt cattttgagt
     2161 gatatgatag gactatcgtt agcattcgaa aggagaggca tcatggctag aacggttgta
     2221 ggagttgctg caaatctatg tcccgtagac gcagaaggca aaatcattca ttcatctgta
     2281 tcttgtagat tcgcagagat cattcgtcaa gtcggtggtc tccctttagt cattcctgtt
     2341 ggtgatgagt cagttgtacg tgattatgtg gaaatgattg acaaactcat tttgacagga
     2401 ggccaaaatg ttcatcctca gttttatgga gagaaaaaga ccgtcgagag cgatgattac
     2461 aatctggtcc gtgacgaatt tgaattggca ctcttgaagg aagcgcttcg tcagaataaa
     2521 ccaattatgg caatctgtcg cggtgtccaa cttgtcaatg ttgcctttgg tggaaccctc
     2581 aatcaagaaa tcgaaggtca ctggcaagga ctacctttcg ggacatctca ctctattgag
     2641 acagtagaag gaagcgtggt ggctaagcta tttgggaaag aaagtcaggt caactcagtc
     2701 catcgtcaaa gcattaaaga tttggcacct aatttccgtg taactgctat tgattcaaga
     2761 gaccaaacta ttgaagcgat tgagtctatc gacgagcacc gcattatcgg tttacagtgg
     2821 catccagagt ttctggttaa tgaagaagat ggcaatttag aattatttga gtatttattg
     2881 gatgaactgt aacaattgga acatctggtc gttctttctt ttattttttc aaaattttta
     2941 gaatgtggta ttttacgcaa acgtttgaat tctgataaaa attggagaaa ttcgacaaaa
     3001 aaacttgaaa aaaacgaagg taagcgttat gatagaaaag aagaaatatt ggaggaagaa
     3061 catgtcacat attaaatttg attattcaaa agttttagac aaatttgttg caccacatga
     3121 agtggaatac atgcaatcac aagtaacagc agcagatgaa ttgatccgta aaggaactgg
     3181 tgctggtagc gacttcttgg gatggttgga ccttcctgaa aaatacgacc gcgaagaatt
     3241 tgaccgcatc ttgaaagctg ctgagcaaat caagtcagac agcgatgttt tggttgtgat
     3301 cggtattggt ggatcttacc ttggtgcaaa agcagcaatc gacttcttga atcaccattt
     3361 tgctaacttg caaacaaaag aagaacgcaa agctccacaa atcctttacg caggaaactc
     3421 aatctcatct acttaccttg ctgacttggt agagtatgtt gcagacaaag acttctcagt
     3481 aaacgtaatt tctaaatcag gtacaacaac tgaaccagcg attgctttcc gtgtctttaa
     3541 agaactcttg gttaagaaat acggtcaaga agaagctaac aaacgtatct atgcaacaac
     3601 tgaccgccaa aagggtgctg ttaaggttga agcagacgct aacggttggg gaacatttgt
     3661 tgttccagat gatatcggtg gacgcttctc agtattgaca gccgttggtt tgcttccaat
     3721 cgcagcatca ggagctgaca taaaagctct tatggaaggt gcgaatgcag ctcgcaaaga
     3781 ctacacttca gacaaaatct ctgaaaacga agcttaccaa tacgcagctg ttcgtaacat
     3841 cctttatcgt aaaggctatg caactgagat cttggtaaac tatgagccat cacttcaata
     3901 cttctcagaa tggtggaaac aattggctgg tgaatcagaa ggaaaagacc aaaaaggtat
     3961 ctacccaact tcagccaact tctcaactga cttgcactca cttggtcaat ttatccaaga
     4021 aggaactcgt atcatgtttg aaacagttgt ccgtgttgac aaacctcgta aaaacgtgct
     4081 tattcctact ttggaagaag accttgacgg acttggttac cttcaaggaa aagacgttga
     4141 ctttgtaaac aaaaaagcaa ctgacggtgt tcttcttgcc cacacagatg gtgatgtacc
     4201 aaacatgtat gtgactcttc cagagcaaga cgctttcact cttggttaca ctatctactt
     4261 cttcgaattg gcaattgccc tttcaggtta cttgaatgct atcaacccat ttgaccaacc
     4321 aggtgttgaa gcttataaac gtaacatgtt tgcccttctt ggaaaaccag gatttgaaga
     4381 attgagcaaa gaacttaacg cacgtctata atagaagaaa agagtggttt gcccactctt
     4441 tttactctct ttatccatag aaattggact cagccaagac ttgtgatata atatagaaag
     4501 caaaaaggca gacgcctaga taataggaga aactatgtca aaagatatcc gcgtacgtta
     4561 cgcaccaagt ccaacaggac tactacacat cggaaatgct cgtacagcat tgtttaatta
     4621 cttgtatgcg cgccatcatg gtggaacatt tctcatccgt atcgaagata ctgaccgtaa
     4681 acgccatgtc gaggatggtg aacgttcaca acttgaaaac cttcgctggt taggcatgga
     4741 ttgggatgaa agtccagaat cacatgagaa ttatcgccag tctgagcgtt tggacttgta
     4801 tcaaaaatac atcgaccaac tattagctga aggaaaagcc tacaaatctt acgttacaga
     4861 agaagagttg gcagctgaac gtgaacgtca agaagtagct ggcgaaacac cacgctacat
     4921 caatgaatac cttggtatga gtgaagaaga aaaagcagct tacatcgcag aacgtgaagc
     4981 agcagggatc atcccaactg ttcgtttggc tgtcaatgag tcaggtatct acaagtggca
     5041 tgatatggtc aaaggcgata tcgaatttga aggtggcaat atcggtggtg actgggttat
     5101 ccaaaagaaa gacggttacc caacttacaa ctttgccgtt gttatcgatg accacgatat
     5161 gcaaatctct catgttatcc gtggagatga ccatattgct aatacaccaa aacagcttat
     5221 ggtctatgaa gctcttggtt gggaagctcc agagttcggt cacatgacct tgattatcaa
     5281 ctctgagact ggtaagaagt tatctaaacg tgatactaat acacttcaat ttatcgaaga
     5341 ttaccgaaag aaaggttacc ttccagaagc agtctttaat tttattgctc ttcttggttg
     5401 gaacccaggt ggtgaagatg agattttctc tcgtgaagaa ctcattaaac tttttgatga
     5461 aaaccgcctc agcaagtcac cagcagcctt tgatcagaaa aaactagact ggatgagcaa
     5521 tgattatatc aagaatgcag accttgaaac catctttgaa atggcaaaac cattcttaga
     5581 ggaagcaggc cgtttgactg acaaggctga aaaattagtt gagctctata aaccacaaat
     5641 gaaatcagta gatgagatta tcccattgac agatcttttc ttctcagatt tcccagaatt
     5701 gacagaagca gagcgcgaag tcatgacggg tgaaacagtt ccaacagttc ttgaagcatt
     5761 caaagcaaaa cttgaagcga tgacagatga taaatttgtg acagaaaata tcttcccaca
     5821 aattaaagca gttcaaaaag aaacaggtat taaagggaaa aatcttttca tgcctattcg
     5881 tatcgcagtt tcaggcgaaa tgcatgggcc agaattacca gatacaattt tcttgcttgg
     5941 acgtgaaaaa tcaattcagc atatcgaaaa catgctaaaa gaaatctcta aataaagagg
     6001 atacaataat ggacatctgg gaaaagatgt acgaagaagc acagaaacta tacaatccac
     6061 atgaagtatc agactttgtt tatgccaatc atgttgtagc tgcagtagaa gcagaagatg
     6121 gacaaatatt tacaggattc tgtatggagg gaacctgtgg tgttttccat ctctgcgcag
     6181 aacgggcggc cctcttcaat acgtaccaat tttcaggaca aactaaggtt aagaaagtat
     6241 tagcctttcg agacaaacca ccttatggtg gaagttcagc catgccttgc ggtgcttgta
     6301 gagaattcct tttagagctt aatgctgaaa ataaagatgc agaattcatg atggactata
     6361 atataagaaa aacagttaaa gtcgcagaac taatccctta ttggtgggga gaagaacgtg
     6421 cttctaagtt taatgagcga tagaagagtc agtataattc tggctttttt tactataagt
     6481 tgaaaaaat
//
LOCUS       DAVBGY010000084         5993 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVBGY010000084 DAVBGY010000000
VERSION     DAVBGY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.084"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            120..1889
                     /locus_tag="VQ620_001913"
     CDS             120..1889
                     /locus_tag="VQ620_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HET2764490.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGASPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            1913..3868
                     /locus_tag="VQ620_001914"
     CDS             1913..3868
                     /locus_tag="VQ620_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2764491.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            3870..4307
                     /locus_tag="VQ620_001915"
     CDS             3870..4307
                     /locus_tag="VQ620_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2764492.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKNDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            4369..5505
                     /locus_tag="VQ620_001916"
     CDS             4369..5505
                     /locus_tag="VQ620_001916"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HET2764493.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPTQSHEDSLFVMVEPFNEWVVETKRLKNPDLRLEDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERLENPFIADEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
BASE COUNT         1831 a         1011 c         1226 g         1925 t
ORIGIN      
        1 aagccttttt gcccagcctc gtcaaaagta atgttttgac acaaaatctg tgacaaaact
       61 ttagttttaa aggtttttaa ctttgtatat actagtttta agaaaaggag gatgatctaa
      121 tggaagaaaa agtatcattg aaagtcaggg ttcaaaaact agggacatcg ctttcaaata
      181 tggttatgcc caatattgga gcatttattg cttggggagt attgactgcc ctctttatcg
      241 ctgatggcta tctgccaaat gaacagttag ctactgttgt tggtcctatg ttaacgtatt
      301 tattgccaat cctgattggt tacacaggtg gatatatgat ccatggccaa cgtggtgccg
      361 ttgtaggagc tattgctact gttggtgcaa tcacaggttc tagtgttcct atgtttatcg
      421 gagctatggt aatgggccca ctgggaggat ggactatcaa gaaatttgat gagaagttcc
      481 aagaaaaaat tcgtcccgga tttgaaatgt tagttaataa tttctcagct ggtctcgttg
      541 gttttgcatt attgcttttg gctttctacg caatcggtcc agtcgtatcg actcttactg
      601 gagctgttgg gaatggtgtt gaggctattg tcaatgctcg cctccttcct atggctaata
      661 ttatcatcga accggctaaa gtccttttcc tcaataatgc cctcaatcat ggcattttta
      721 ctcctctggg agtagaacag gtagctcaag ctggtaagtc aattctcttc ctattggaag
      781 ctaatcctgg accaggcctg ggaattctat tagcttatgc tgtattcggt aaaggttctg
      841 ctaaatcttc ttcttggggg gcaatggtta ttcatttctt cggagggatt catgaaattt
      901 actttcctta tgttatgatg aagcctactc tatttttagc tgctatggca ggaggtatct
      961 ctggaacttt tacttttcaa ctcttagacg ctggtcttaa atctccagct tcaccaggtt
     1021 ctattattgc gattatggct acggcgccaa aaggtgtttg gccccatcta aatgttcttt
     1081 taggtgtttt agtggcagca gttgtttctt tccttgtagc agcccttatt cttcatgcag
     1141 acaagtcaac tgaggattcg ctcgaagctg ctcaggcggc tacccaagca gctaaggctc
     1201 agtctaaagg tcagttagta tcaacttctg ttgatgcagt tgtttcgaca gactcagtgg
     1261 aaaaaatcat tttcgcctgc gatgctggta tgggaagctc tgctatggga gctagtattc
     1321 ttcgagataa ggttaaaaaa gcaggtctag agattccagt atctaatcag gcaatctcaa
     1381 atttgcttga tacaccaaaa acattaattg ttactcagga agaactgaca ccaagagcta
     1441 aagacaagag tccaagtgct attcatgttt ctgttgataa tttcttagcg tcccctcgtt
     1501 atgatgaaat tgtagcttca ttaacaggag cttctccaat agcagaaatt gaaggagata
     1561 taccaacttc agcaccagta gatagtcagg aaattgacct taaccatatt gatgctgtag
     1621 tagttgctta tggtaaagca cagggaactg caactatggg ctgtgaaacg attcgggcta
     1681 tctttagaaa caagaatatt cgtattccag tttctactgc caaaatttca gaattaggtg
     1741 aatttaattc taaaaacata atgattgtaa caactatttc tttacaggca gaagtgcagc
     1801 aagcagcacc gaattctcaa tttcttattg tggatagttt agtaacaaca ccagaatatg
     1861 acaaaatggc tgctagaatg tacaaataga actagaggtt tctaaattac gaatgctatt
     1921 aaccaaacga gaagaacaat tattgaaggc tttcctacat gtagggaagc tttcaatgca
     1981 agatatgact gaaatcttac aggtttcatc tagaacaatt tatcgaactt tatcagattt
     2041 gacagatagc atggagcaat atggaatcga aataacgaag catgggaaat actatatttt
     2101 gactggagag ttggatgatt tgccgacaga acttgaagtg ttagttgagt atagtcccca
     2161 agaaagacaa gagttgatta cctatcgcct tctgactgag agcggttttg tcaccaatga
     2221 agcattgcaa gagtgcacga aagtcagtaa tgtaactatt attcaggata tttcagatat
     2281 tgataagcgt cttttagact ttgatctgaa aattgaacga caaaaaggtt atcggatttc
     2341 tggtgattca gttggtaaga gaagattttt ggctatttta ctgacaaact gtatctcagt
     2401 agcagatttt tcaaccggta attttgggag ctttgatatt ttagaagcag atagaactag
     2461 gctggccagt cagattgtta ataagcaact gtcaggtttt ccagatatgg atgctaggat
     2521 gaagatgttt tttgcgatct tgttatctct tataggtcag gagcaaaaca ttgaaaattc
     2581 acctaatact agtaagcagg ctttggaaat ttctcaaaaa atttttcaag cttactctaa
     2641 gcagactgca caattttata gtattcagga aattatctat tttgcgagca tcttggatga
     2701 attaatcatt aaacgtcagg acaatccgct ctttacggag aaatttgatg gtgaattttt
     2761 ctacaatatt tcaaatctga ttgatacggt ttccatgtat accaagattg acttttttaa
     2821 ggacaaggtt ttattcaatt ttcttttcca tcatattcgg ctcagtttag gcgtccctat
     2881 cctttttcag ggtgaaaatt tgccagaatc tgtccagatt ttagttgaaa ggaataaatt
     2941 tctttataca gtcatcagtc ttttagtgaa tgatattttt ccgaaatatc ttcatacaga
     3001 gtatgagtat ggcatgattg ccctacattt tatctctagc ttaggccgta gtccagagat
     3061 ttatccagtc cgtgttttgc ttttaacgga tgaacgtcgg gtcactagag atttattagt
     3121 cagtaaaatt aagagtgttg ctccttttgt agagttgata gatattcagt ctctagtaga
     3181 ttaccacagt attgatctca gtcagtatga ttatatttta tctaccaagc cgctgactaa
     3241 tcaggaaatc gatgtaattt ctagttttcc aaccgtcaaa gaattgcttg aattacagga
     3301 acgacttcag tatgtacagg cacatcgtac aattgtcgcg cgtgatgcta tcgctccaga
     3361 gaaaagttat gacttgcaag attatttaat atctagtagt cagcttttga gtcaatttga
     3421 gttggttcaa ttggagaata atcaatcatt tgagcacacg gtagaacaaa tcatccaata
     3481 tcagaagaat gtgagtgaca gagattacct aacaagaaaa ttgttatctc acttccagaa
     3541 tagtcctatg gctattccta atactggtct ggtgctttta catagtcagt ctagcaaagt
     3601 aacaacaaat agttttacta tgtttgaact caaactacct atctccgcat tgtcaatgaa
     3661 acgagaggaa gaagaggtca aaaggtgtct gctaatgcta atgtctaaag aagctagcga
     3721 ggaagctaga gatttaatga cagctattag tcagtcgatt attgaaaatc atctttatac
     3781 agagatttac aagacgggaa atcaatccat tatttatcag atgctaaata ctatttttaa
     3841 cgaaaaaatt aagaaattgg agaactaata tgaaacttga aaaacatttg attaagctta
     3901 ataaacaatt ttctaacaag gaggaagcta tttgttattg tgggcaagtt ctttatgagg
     3961 gtggatatgt taatgaagac tatattgaag ccatgattga gcgagataaa gagctatctg
     4021 tttacatggg taactttatc gccataccgc atggaacaga tgcagcaaaa aatgatgtcc
     4081 tcaagtctgg tattacagtc gttcaagtcc ctagaggggt tgattttggg aatgtatcta
     4141 accctcaagt ggcaacggtt ctttttggta ttgctggtat tggtaatgaa cacttagaaa
     4201 ttattcagaa aatttctatc ttctgtgcag atgtagataa tgttcttaaa ctagcagatg
     4261 ctcagtcaaa agaggaagta ttgcgcttat ttgatgctgt tgaataattg aatttcgtca
     4321 tttgtcatct agtatatatg tccctcaaat aggaaaagga gaaattgaat gaaacattct
     4381 gttcattttg gtgccggtaa tatcggtcgt ggttttatag gtgaaattct atttaaaaat
     4441 ggtttccata ttgattttgt ggatgtcaat aatcagataa ttcatgctct aaatgaaaag
     4501 ggcaagtatg aaattgaaat tgcacagaaa ggacagtctc gtatagaagt aactaatgtg
     4561 gctggaatta atagcaaaga acatcctgag caagtcattg aagcgattca aaagacggat
     4621 attattacta ctgcaatcgg acctaatata ctccctttta tcgctgaact tctagccaaa
     4681 ggaatcgaag ctcgccgagt tgcaggaaat acacaggcat tggatgttat ggcctgtgaa
     4741 aatatgattg gcgggtctca atttctttat caagaagtca agaaatattt aagtccggaa
     4801 ggtttgacat ttgctgataa ctacataggt tttccaaatg ctgcagtaga caggattgtt
     4861 ccaacacaaa gtcacgaaga ttcccttttt gttatggtcg agccctttaa tgaatgggtc
     4921 gtggaaacca agcgtcttaa aaatccagat ttacgtctag aagatgtgca ttatgaagaa
     4981 gatttagaac cctttattga gcgaaaactt ttttcagtca attctggaca tgcaacttca
     5041 gcttacattg gtgcgcatta tggtgccaag acaattttgg aagctcttca aaatcctaat
     5101 atcaaatctc ggattgaatc tgtattagct gaaattcgga gtctcttgat tgccaaatgg
     5161 aactttgata aaaaagaatt ggagaattat cacaaagtca ttatagaacg gcttgaaaac
     5221 cctttcatag cggacgaggt tagtcgcgta gctcgtactc caatccgaaa attaggctat
     5281 aatgaacgat tcatccggcc gatacgtgaa ttgaaagaac tcagtttgtc atataaaaac
     5341 ctacttaaaa cagttggcta tgcctttgac tatcgcgatg taaatgatga agaaagtatt
     5401 cgattaggtg aattgttggc taaacaatca gtcaaagatg ttgttataca agttacaggt
     5461 ttagacgacc aagaattgat tgagcaaatt gtagagtata tttaatcttt ttcgaaaatc
     5521 tcttcaaatc aggttagcat cgctttgtct taggcatatg ttactgactt cgtcagttct
     5581 atctacaacc tcaaagcagt gctttgagct gactccgtca gtcttatctg caacctcaaa
     5641 acactgtttg agttatctgc ggtaatcttt ctagcttgtc tttgattttt gttgttattt
     5701 ataaggtaaa agaagctgga caaaaagtct tcaaaatcgg aaaaaggcag cctatcgggt
     5761 gttcaaaaat cttgatagga tgtcctttat tatggaaagc cttattggat tttctcctca
     5821 gattgagttt ttgatcagct ttatgagata ggttttgcta gagatgtagc ccatcatgtt
     5881 atttttatgg actgtgggaa aattgttgaa aaaaataatg cccatcaatt ctttagtcgt
     5941 ccaagagaag aacgaaccaa gcaattttgg aacgaattct ttcgaatgcg atc
//
LOCUS       DAVBGY010000085         5278 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVBGY010000085 DAVBGY010000000
VERSION     DAVBGY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.085"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            51..1100
                     /locus_tag="VQ620_001917"
     CDS             51..1100
                     /locus_tag="VQ620_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET2764494.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGSNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(1200..5063)
                     /gene="spuA"
                     /locus_tag="VQ620_001918"
     CDS             complement(1200..5063)
                     /gene="spuA"
                     /locus_tag="VQ620_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HET2764495.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPGNANASLEKTEEKPATEPT
                     TPAASPADPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQ
                     GLWTWDDVEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNL
                     TGDKSVEKLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVK
                     NPSSAQWPDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFT
                     DLKNHSQIFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKH
                     SNITNHLGNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDE
                     TYSYDGKLGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTW
                     KQTLDSTNKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVA
                     KAAFVDPAKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEA
                     FIEKLDYLKDLGVTHIQLLPVLSYYFVNELKNHEHLSDYASSNSNYNWGYDPQNYFSL
                     TGMYSSDPKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFM
                     DADGTPRTSFGGGRLGTTHHMTKRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEA
                     YKAARALNPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGY
                     PNEGQPAFITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSI
                     KKDPSKAENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRNPAYRTPVAEDK
                     VPNKSHLLRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKG
                     LIALRQSTDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVN
                     ADEKAREFNLGTAFAHLRNAEVLADENQAGSVGIANPKGLEWTEKGLKLNALTATVLR
                     VSQNGTSHESTAEEKPDSTPSKPEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENK
                     PSQATADSQAEQPAQEAQASSVKEAVRKESVENSSKENISATPDRQAELPNTGIKNEN
                     KLLFAGISLLALLGLGFLLKNKKEN"
BASE COUNT         1305 a          997 c         1269 g         1707 t
ORIGIN      
        1 ttagaataat ttttcaaatt gaaggacaga gatagacaag gagaatcaca atggtagaat
       61 tgggaatttc aacatttggg gaaataacgg agcttgaagg gactggacaa acttacagtc
      121 atgctgaacg cattcgccag ttggtggcag agattgagct ggctgacaag gttggtttgg
      181 acgtatatgg gattggcgag caccatcggg cggattttgc agtatcagcc ccagagattg
      241 tcctggccgc tggggcagtc aataccaaga aaatccgttt gaccagtgca gtcagcattc
      301 tgtcaagcat ggatccgatt cgtttgttcc aacagtatgc cactatcgat gctttgtcaa
      361 atggacgatc ggagattatg gctggaaggg gctctttcac ggaatctttc cctctgtttg
      421 gttatgactt gaaagactac gactctcttt ttgatgagaa attagacttg cttcagttag
      481 tcaatgaaaa gaccaagtta gactggcaag gtcgattgac tcaaacgatt gctggtaaag
      541 aagtttatcc tcgtccagtt caggacaaat tgcccttgtg gatagctaca ggtggtcatg
      601 tcgaatcaac agtgaagatt gctcaggctg gtctaccgat tgtatatgct attattggtg
      661 gtaatccacg ttattttaaa aagttgattc aggcttatcg tgagattgga agcgaagcgg
      721 gccatgctga caaagacttg aaagtgggag cccattcttg ggggtggatt gcggaagatg
      781 gtgagcaggc ggtgaaagat tatttccatc cgactaagca agtggtggat gctatttcca
      841 aagaccgtcc gcactggcag gaattgcgtt atgagcaata tttggagcag gtagggtcaa
      901 atggcgccat gtttgttggc aatccagatc aggtggccga aaaattgatt cgcatgatag
      961 aggatttagg attggaccgc ttcatgcttc atctaccgct tggttctatg cctcatgacc
     1021 aggttctgag agctattgaa ctcttcggaa cacaagttgc acccaaagta cgagcttatt
     1081 ttgctatgaa agaggcttaa taaaaaatcc taatcaagtt tattaggaca acaaatattg
     1141 tacaaaaatt aatcccccga gctataatgc ttgggggatt tttctatagg agggctagcc
     1201 tagttctctt ttttattttt tagtaagaaa ccgagaccaa ggagcgcaag gaggctgatt
     1261 cctgcaaata ggagtttgtt ttcgtttttg attcctgtat ttggaagttc agcttgtcta
     1321 tctggggttg cagatatatt ttccttgcta gagttttcta ccgattcttt tcgaaccgct
     1381 tcttttacag atgatgcttg tgcttcttgt gctggttgtt cagcttgtga atcagctgta
     1441 gcttggctag gtttattttc cgcatcagct actttggcat ctggtttagt agaatcaggt
     1501 ctagcttctg gagctgggtc ttgatgtgca gggtgagaag cttcattttg atgttcaggc
     1561 ttggaagggg ttgagtctgg tttctcttct gcagttgact catggctagt tccattttga
     1621 gagactcgaa gaacagtagc tgtaagggca ttcaatttca agcctttttc agtccattca
     1681 agtcctttcg ggttggcaat tccgactgat cctgcttggt tttcatctgc caaaacttcc
     1741 gcatttctta gatgtgcaaa ggcagttccc aaattaaatt cgcgagcttt ttcatccgca
     1801 ttgacaaaga ctgcgtagat atcgccgttt ggagcagtga tttgatagcc aatcactaca
     1861 tcctcttttt ccacaccatt ttggcctggg acagtgatga ggtggacacg gtctttgata
     1921 tcttgaagac tcttaagtcg gaaggcatct gtagattgac gaagggcaat caaacctttc
     1981 atatagtcac ggctcttgac attttcagga taagctttac catctgtagc cttagtccag
     2041 tcaaacttgt tgactgcatc actagaatcg taagagtcat ggatgaagta aggatagtca
     2101 aatgggttgc cgtccttatc acgcaacaag tgagatttgt ttggaacctt atcctctgct
     2161 acaggagttc tgtaggctgg gttacggaat tgtttggtac gtccatattc ctggccagag
     2221 tggataaacg gagttccttg agctgtcaag accatgagat ttccaagtct caaacgacgg
     2281 tggatttcag catagttctc agccttgctt gggtcttttt tgatagactg ggcaatgatg
     2341 tcaaagaggg tcaagttatc atgggctgcg atgtattgga tgacatctcc agggctgtca
     2401 gcttcaaagt tagttggctg agcaatgaga tttttaaaga tagtgttaac atcacgcttg
     2461 ccacctgtga taaaggcagg ttgaccttcg tttggataac cagatttgag gttgttacgg
     2521 atgtcatctg aaaagacagc gacagtatcg gtatgtttca tccaatcttg gtcagcagct
     2581 ttagtaggca tgttttcatc accggcatag gttctccaac cttcgcctaa catgattaga
     2641 tttggattga gggcgcgtgc agccttgtaa gcttcttcga tagaagcggc gtcatggtct
     2701 cccatcatat cgaaacggaa tccatccact ttgtaggtat caactaggta tttgatagag
     2761 tcaactagga ggcgtttggt catatggtgg gttgtcccca agcgtccacc accaaagcta
     2821 gttcgaggtg tgccatcggc atccataaag tggtagtagt ttggctctag gtcttcaaag
     2881 atatcgactt tggctgtgtg gttataaacg acatctagga tagctcccat accacgtttg
     2941 tggatttcgt tgatgaggtt tttaaattct gcgattcgtt tttctggatt cttaggatcg
     3001 cttgagtaca taccagtcaa ggagaagtag ttttgagggt catatcccca gttgtagttg
     3061 ctgttgcttg aagcatagtc agacaagtgt tcatggtttt tcaattcatt gacaaagtag
     3121 taagacaaga ctggaaggag ctggatatgg gttacaccca agtctttgag atagtctagt
     3181 ttttcaatga aggcttcaaa agtcccaaat ggtttggtca agtcttttgc aatggcagga
     3241 tctgaagtga agtcacgcac atgagcttcg tagataacgg cgtcttcacg agtcttgaaa
     3301 ttgtgaatct taccataagt caagtcttga ggtccgagtt tagctggatc tacaaaggcg
     3361 gctttagcca ctttatgggc atcgtcaatc ttggcatcgt cgctattcca agcagcaaga
     3421 gatttagcgt aaggatcgag tgcaagaaca gttttacctt gacgctcgat ttggtattga
     3481 taatagtagc cagtgaaatc tgtgattccg agtttgtttg tgctgtctag agtttgtttc
     3541 caagttcctc tttccccttt ttcgagagcg acagttccaa ctactttgtc agggtcattc
     3601 ttgtcgtaga caacaacaga aaccttatca gcacttggtg accaaagggt caaatcaact
     3661 tgttttcctt cttcttttag gtcagctccc agtttgccat catagctgta tgtctcatct
     3721 ttcaggcgcc agcttgtttt ggtagtgaat tggtcggaat tgtagctaac agtataagga
     3781 tgttttgtgt cagagaaatc tccgctgtag gtcactttct taccagcttc atcgattgca
     3841 acatcggtaa tagttacctt gtttcctagg tgattagtga tgttggagtg tttgaggata
     3901 tcttcttttt tagcaccgac aagtgttgaa aagctacttt caatgctaga agtgcctacg
     3961 tgttgggctc ctgtcatacg gatatcatgg acatagtatg gatttgtgta aatcgattca
     4021 tcatcgtctt ttaggaaaat ttggctatga tttttcaaat ctgtgaactt ataattttct
     4081 ttacggattt tcacgtcgtc tccttgtttg ctctcatcta gtaataaaaa tccaaattct
     4141 cttgcggctt cattaagagg aatgtcgata tagcggccat atttgcctgt agccgtaaag
     4201 tctgttccgt caggccattg agcgctactt ggatttttca catctcccca gtaccagaga
     4261 gatttcttgt catagttgcc atctgtgcgg tagtagttga cgcgaacagt tcctgcaggc
     4321 tgtggctcgt aagagaaaac cttgtaatct tggtctaacc aagcctcgtt catttttgga
     4381 actagttttt ctacagattt atcgccggtt agattttttc cagctgtatt gttgatgagg
     4441 aagctaattt tcttggcttg ttctcccttt aatttgacat ctaggtaata gccgtagtca
     4501 tctttcttgg catccttgaa ggacaaagct ccgtttggcc agttttcaga tggtttttca
     4561 acatcgtccc aagtccatag tccttgagca tccttgtttt cttcaggaag ttttttgaca
     4621 tggatacgga agtagttatc ttcgatgggc tcttctgcct tagtttcagt tgttactgga
     4681 ctagtagaag tagttggctc acttgaacga tcttgtccag tttgtggtgc tggatcggct
     4741 gggcttgcag caggagttgt tggctctgtt gcaggttttt cttctgtttt ctctagggaa
     4801 gcgtttgcat ttccaggtga agaaatatca ctttcgttct taccagagcc agctgctgta
     4861 tcagtaagag gttgggcaag ggtggttgta tttctgttgt tgggttcgtt agtggttggt
     4921 gtactttcgt cggatgagat agttggtgta gccatggcaa gcaagacaag gcttgcgccg
     4981 ataaggacag aaccagttcc atttttgagg gaacggatgc tgtagaccat ttttttctca
     5041 gtgtgagatg gtgtttttct cataatgatt ctccaatcaa taaatttcta tatcagtata
     5101 gcatgtagta aaacaatatg caagcgtttt ctttgatttg tgaaaaaaat tcttactttt
     5161 gagaagtttt ctgtactatt ttatatatca gtataaatga cggagataaa aatgtcacat
     5221 aagccaagcc gtattattag gaagatgatg tgttcccagc tatttttatt cagaaatt
//
LOCUS       DAVBGY010000086         5114 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVBGY010000086 DAVBGY010000000
VERSION     DAVBGY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.086"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(449..2728)
                     /gene="pulA"
                     /locus_tag="VQ620_001919"
     CDS             complement(449..2728)
                     /gene="pulA"
                     /locus_tag="VQ620_001919"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HET2764496.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(2840..4798)
                     /gene="ligA"
                     /locus_tag="VQ620_001920"
     CDS             complement(2840..4798)
                     /gene="ligA"
                     /locus_tag="VQ620_001920"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET2764497.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(4891..>5114)
                     /locus_tag="VQ620_001921"
     CDS             complement(4891..>5114)
                     /locus_tag="VQ620_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2764498.1"
                     /translation="FFTQVQTKVESEFLGRVLSTIFTLAILFMPIAKGFMTVLPSVHL
                     SSFLIIGSGVIILSCISFIYVRTHFEKLI"
BASE COUNT         1505 a         1129 c          943 g         1537 t
ORIGIN      
        1 cttgattttt caaagtatac tcaataaaaa taaaaaaatc cactacgtca aggcgaggct
       61 aatgtggttt gaagaaattt tcgaagagcg tgaatgagta tcatctatag taaaataaaa
      121 aaactgaaca atttggttgg ggacagccaa accaatttct cacaatgttt cagaaacaag
      181 ggtgtgctat tccaatttca gcctactata actgtcatag attgctgaaa caaagtctag
      241 gtaaaagtct tcataataaa aagacctcct atcaaatgtt caaaaacttt gataggaggt
      301 cttgtttcgt gaaaatattt atcaaatttt ctatacaagt gagctgttag ccaggttctt
      361 tctattcttt caatttcaat gaatggattt tttaccaata ctcataactg ggaatttttc
      421 tgtctaaaaa tagcgagata gatggtattt ataaaacact caagacagct agactaatat
      481 catttaaaac attatcttct tttgagcgac tgttggttac caacatagct aaatttcctg
      541 cattttcaaa ttgatagggt tctgatttag cattcacaac taccaagagg tgttctttgc
      601 cgtgaacttc atagataagg cagccgctat gttcaatcgc agaatgcaca aagacatgat
      661 ggtaaatttc atcatagcta gagtaagaaa aggcaccagt ttttgtcttc aatcggatga
      721 cttgacggat aaactcaata ctgtcttgac gctcattaat caagttccag ttcacttggt
      781 tcacactgtc aggagcatta tagctattca tcgcacgctc tctatcatca tgggtcaact
      841 caccattttc accagtcgca accagtttgg tacgaccaaa ttcttgaccg atttccataa
      901 aggccatccc ctgcatgagc agattcatgg ctgtggcagt ttcgaccttg cgcatgattt
      961 gctctgaact ttggtctgga tgaagggttg ccaataaatc gtgaagattg taattgtcat
     1021 gggcttctac atagttaagc acctgatttg gatgtgtata gcttcctaat tcacgacttc
     1081 ctaggattgc tttagctaga attggctctg tcgcagcacc actgacaaaa cctgacttga
     1141 tagcaccata aacttctccc cctttgacag catcgcgctg attgtcatta aagaaaccaa
     1201 tatttggcat ctggtaggca ttgtccttct tggccttatc ataaggggca agacctgttc
     1261 ccatatccca tccttctcca tagaggataa tgttggagtc aatttcatcc aagctttgac
     1321 gaatcatctg catggtcttg acatcatgaa tccccatcaa gtcaaaacgg aagccgtcaa
     1381 tattatattc ctgcacccag tatagaagag aatcaatcat atacttgcga aacatttcgt
     1441 gttcactggc tgtttcattt ccaacacccg ttccattctg gaaggtacca tctggattca
     1501 tacgataata gtaatcaggg actgttgttt ggaatggtgc atcaacaact gagaaggtat
     1561 ggttatagac tacatccata atgactccaa tacccgcatc gtgataagct tgaaccatca
     1621 ccttcaaatc acgaatgacc tgagctggat catctggatt agttgaaaaa ctagtttctg
     1681 gcgcgttata gttttgtgga tcataacccc agttgtaggt tacatttcca tcctcatcgt
     1741 attctttatg acggtctgca attggttgca attgaacata attgtagccc agcttcttga
     1801 tgtaatcaaa agcagttgac tggccgtatt ggttaactgt tccagcctga gcagcaccca
     1861 agaaagttcc tcgaagatgt tcatctacac ccgatgtagg tgatttagtc aaatcacgaa
     1921 tatgcatttc acagataact gccttacatg gattttccaa gcgccaagta gcctccgaac
     1981 cgtgcttaac ctcgaagttt tcaacttgct tttctacatg gctcagaata gctgaacgtt
     2041 tgccatcagg gctggtcgcg attgtataag gatcacgtgt cagtgtttgg tgatgaggga
     2101 attggacttg atactgataa gtcttaccta ccaaatcttc ttcaacatcc aaactccaga
     2161 caccgattgt attgtcctta tgattataag agtagctatt gcctcttttc atctcaaaag
     2221 tcttccaaac gggtgcatca ttagcagctg attcataaac gacaacttgc acttctgtcg
     2281 ctgtaggtga ccagagggaa aaatgagcct gattgtcctc tacacggcaa cccaattctc
     2341 cttggtaacc ccaatgatga tcaaaactag cactgttaat ggccttatca aaggcaaaag
     2401 gattttgatt tttatagaaa ggactggcaa tagcaggatt ttcagagtaa taaatcctat
     2461 catcgccttc caaaatccag acctctgtta ataggggata gtgattaaaa cggatagaat
     2521 attctttact agtttgacct gtatgaacca caaaattcaa gctttctata acatgtgaac
     2581 ttgggtgttc aaagctaaat aaagctccaa aataatcttc tttgtaggtt agcaaatcaa
     2641 ttcgttgatc ctgacttttt acaaaggagc aagtgtcata ttctccattc ttacgatggt
     2701 aatgaatgcg catagggtag ttatacattt tttatttttc ctttttactt tgtttctatt
     2761 tcactaataa atttttgtca atctcgtctc aattaacaga catagtcata ttctctaaac
     2821 tctgttttta aacgatccat tacaaacttt ctagccatgc ctcatctctg acctggatac
     2881 caagttcttg tgctttttgc agtttacttc cagcgtctgc acctaccacg acgaggtcgg
     2941 tctttttaga aacactacct gtcactttgg cacccagact ttcgagttta cttttagctt
     3001 ctgagcgctt gagtcgttcc aattttcctg tcaataccac ggtcaaacct gacaaggccg
     3061 catccgctac taccgtctgt cctttatagt ccagattgac cccagtttct ttcaattctc
     3121 tgagcagaat ttcagagcct tctgtcgcaa aataagtctg aagacttttg gcaatcacgc
     3181 cacctagact ttcaatacta gccacttcct ctgaatctgc ctgagacaga ttttcaattg
     3241 aatggaaata ttgaagtaaa agctgactag ccttgcttcc gacatgacga attcccaaac
     3301 caaataagag cttctcggca gaattttcct ttgatgcttg gatagcctga tacagtttag
     3361 cagcggactt ttccttaacc ccctctaaaa ggaggaaatc ctcttcttgc aaacgataaa
     3421 tatccgccac atccttgact aaattagcag caaaaagctt ctcaacaata gatggaccaa
     3481 ggcctgtaat attcatagca tcacgagaag caaagtgaat caagccttcc atgatttgag
     3541 cagggcaacg cggattgata caacgtaggg ccacttcatc ttcaaagtgc aacaagtcag
     3601 agttacaact tggacagttt gtagggatat ctagtttttc ttcagaaacc cgtttggact
     3661 ctaccacacg taaaacggca gggatgatgt caccagcctt atatacaatg accgtatcgt
     3721 cttttcggat atctttttca gcaatataat ctacattgtg cagggtcgca cggctaacag
     3781 tcgtaccggc aagttgtact ggtgttagat tagcagttgg agttacaaca ccggtacggc
     3841 caactgtcca gtcaactgat aagagttgag cttctttttc ttcagcaggg aacttgtagg
     3901 ctactgccca ctttggagcc ttaacggtaa aaccaagttc ttcttgactt gctaggtcgt
     3961 tgaccttgat taccactcca tcaatatcgt aaggcagatt ttcccgttcc tgtcctactt
     4021 cttggataaa attccagatt tcatctatgt tttcagccaa gattcgctta ggattgacca
     4081 caaaacctag ttgttctagg tacttcaaac ccttttcttg gctatcacga gttgaagggc
     4141 tggcttcttg atagagaaac gttgcaagat tacgcttggc aactactgct gtatccaact
     4201 gacgcagagt tcctgctgcc gcattacgag gattagcaaa ttcaggctct ccattttctt
     4261 ggcgcacttg gttaacttgg tcaaaggaag cgcgtggcat gtaacattcc ccacgaactg
     4321 tgatatctag ttcttctggc aaagtcaaag ggatgtcctt aacacgcttg aggttttctg
     4381 tgatattttc accaattgaa ccatctccac gtgttacccc agcaaccaaa atcccctttt
     4441 cataagtcag cgagatagat aagccatcga ttttcagctc acaaatatag gtcggatgag
     4501 ccacttcctt acgaacacgc gcatcaaaag catctagctc ctcacgtgaa aaagcatcct
     4561 gcaaactata aagaggatac tgatgactgt atttttcaaa accatctaaa accttgccac
     4621 caacacgatg agtcggactg tctgctagca cttgctctgg ataagcagtt tctaactcga
     4681 ccaactcacg gtaaaggcgg tcatactcac tgtctgaaac cgagggatta tcgctggtat
     4741 agtactcagt cgcatagcga ttgagcaaag cgactaactc attcattctt ttattcataa
     4801 gaccatttta ccataaaaca agccctcctc acaaacgaga agggcggaaa aaacacttag
     4861 tttgaaatta tttttgaaac tcaagcaacc ttatatcaat ttttcaaaat gagttcgaac
     4921 ataaataaac gatatacaag acaagatgat aacaccactt ccaattatca ggaaagaaga
     4981 gagatgtaca cttggcaaga ctgtcataaa tccttttgca ataggcataa atagaatagc
     5041 taaggtaaaa attgtactca gtactcttcc aagaaattcg ctctcaacct tggtttgtac
     5101 ttgagtaaaa aagt
//
LOCUS       DAVBGY010000087         4847 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVBGY010000087 DAVBGY010000000
VERSION     DAVBGY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.087"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            21..866
                     /locus_tag="VQ620_001922"
     CDS             21..866
                     /locus_tag="VQ620_001922"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET2764499.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(1013..2074)
                     /gene="dinB"
                     /locus_tag="VQ620_001923"
     CDS             complement(1013..2074)
                     /gene="dinB"
                     /locus_tag="VQ620_001923"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET2764500.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            2422..4746
                     /gene="pflB"
                     /locus_tag="VQ620_001924"
     CDS             2422..4746
                     /gene="pflB"
                     /locus_tag="VQ620_001924"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HET2764501.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKSNFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT         1410 a          996 c          962 g         1479 t
ORIGIN      
        1 tataatatag ggagtaatct atgtatctta ttgaaatttt aaaatctatc ttcttcggga
       61 ttgttgaagg aattacggaa tggttgccga tttccagtac aggtcacttg attttagcag
      121 aggaattcat ccaataccaa aatcaaaatg aagcctttat gtccatgttt aatgtcgtga
      181 ttcagcttgg tgctatttta gcagttatgg tgatttattt taacaagctc aatcctttta
      241 aaccgactaa ggacaaacag gaagttcgta agacttggag actatggttg aaggtcttga
      301 ttgctacttt acctttactt ggtgtcttta aatttgatga ttggtttgat acccacttcc
      361 ataacatggt ttcagttgct ctcatgttga ttatctacgg ggttgccttc atctatttgg
      421 aaaagcgcaa taaagcgcgt gctatcgagc caagtgtaac agagttggac aagcttcctt
      481 atacgaccgc tttctatatc ggactcttcc aagttcttgc tcttttacca gggactagcc
      541 gttcaggtgc aacgattgtc ggtggtttgt taaatggaac cagtcgttca gttgtgacag
      601 aatttacctt ctatcttggg attcctgtta tgtttggagc tagtgcctta aagattttca
      661 aatttgtgaa agccggagaa ctcttgagct ttgggcaatt gtttttgctc ttggtcgcga
      721 tgggagtagc ttttgcggtc agcatggtgg ctattcgctt cttgaccagc tatgtgaaaa
      781 aacacgactt cacccttttt ggtaaatacc gtatcgtgct tggtagtgtt ttgctacttt
      841 acagttttgt ccgtttattt gtataagaaa aaccttgaag gggcaactct tcaaggtttt
      901 atactcttcg aaaatctctt caaaccgcgt cagctttatc tgcaacctca aaacagtgtt
      961 ttgagcagcc tgcggctagc ctcctagttt gctctttgat tttcattgag ctttaaaatc
     1021 cagtcatggt aatccccaat aggcggacac ctctttcttt cttgcttaat tcttcataga
     1081 gttgcagggc tatttggctt atctgactag catcttgtgt tttttgagca agactttttc
     1141 gtttggtaag agttgaaaag tcctcgtagc ggattttcaa aatgacaatt tttccagctt
     1201 tttcttgttg atgtagattg agagcgactt tttctgatag aagagtcagc tcttttttga
     1261 tatcttcctc agcaaggaga atcttcccgt aggttttctc cttgccgatt gatttacgga
     1321 tgcgattgga tttgactgga gagttgtgaa tgccacgagc ctttcgatac agatcatagc
     1381 ctagtctacc aaaacggtct attagggtta cctcaggaac ttcaagtaaa tcagcaccag
     1441 taaaaacgcc catttgatga agacgttcta ctgtcttttt tcctactcca tgaaatttgg
     1501 aaatatccat ttgtttgaga aaatcctcag cctgttcagg tagaatcact gtcaaaccat
     1561 gtggtttttg ataatcactc gccattttag ctaagaattt gttgtaagaa acgcctgcgg
     1621 aagcagttag atggagttct tgccagatat ctttttgaat gaggcgagca attttgaccg
     1681 ctgacttgat accgagttta ttttctgtca catccaaata ggcttcgtca atgctcatgg
     1741 gttcaatcaa atctgtatag cgcttaaaaa tagctcgaat ctggagtccc acagacttgt
     1801 atttctcata attccctgag ataaagacag cctggggaca acgttcataa gcttccttgg
     1861 aactcatggc agaatggaca ccaaaagctc ttgcctcata actacaggta gaaacgactc
     1921 cccgtccacc tgtttgccga gggtcgcttc caataatgac aggttttcct ctgagtttag
     1981 gattatccct gatttccact gcagcaaaaa aggcatccat gtcaatatgg atgatttttc
     2041 ttgacaaatc atttaacaaa ggaaaaatca acatgcctag caccttttta tactcttcga
     2101 aaatctcttc aaaccacgtc agctctatct gcaacctcaa aacagtgttt tgagcaatct
     2161 gcggctagct tcctagtttg cttttcgatt tccattgagt gttactgctt attttctttt
     2221 attataccct tttttctgaa aaaaagaaaa aaggacttta ttttttcaaa aatataatac
     2281 agtttgaaat aaaatataga ctgttttaga aaagaaagtg taaaaatagg gaattttcac
     2341 ttgttgaaat cggttactat atggtatact tgtcttatga atgtaacaga tgactgttac
     2401 tagaaaaaag aggacattaa tatggttgtt aagacagttg ttgaagcaca agatattttt
     2461 gacaaagctt gggaaggctt caaaggcgta gattggaaag aaaaagcaag tgtatcacga
     2521 tttgtacaag ctaactacac accttatgat ggagacgaaa gcttccttgc aggaccaaca
     2581 gagcgttcac ttcacatcaa gaaaattgta gaagaaacta aagcacacta cgaagaaact
     2641 cgtttcccaa tggacactcg tccaacatct atcgctgata tccctgctgg atttatcgac
     2701 aaagaaaatg aagttatctt cggtatccaa aacgatgaac tcttcaaatt gaacttcatg
     2761 ccaaaaggtg gtatccgtat ggctgaaact actttgaaag aaaatggata cgaaccagac
     2821 ccagctgttc acgaaatctt cactaaatat gtaacaacag ttaacgacgg tattttccgt
     2881 gcctacactt caaatattcg tcgcgctcgt cacgcacaca ctgtaactgg tcttccagat
     2941 gcatactcac gcggacgtat catcggtgtt tacgcacgtc ttgctcttta cggtgcagac
     3001 tacttgatgc aagaaaaagt aaatgactgg aatgcaatca aagaaatcga tgaagaaaca
     3061 atccgtcttc gtgaagaagt aaaccttcaa taccaagcat tgcaacaagt tgttcgcctg
     3121 ggtgaccttt acggggttga tgttcgcaaa ccagcgatga acgtgaaaga agcaatccaa
     3181 tgggttaaca ttgctttcat ggctgtctgc cgtgtgatta acggtgctgc tacatctcta
     3241 ggtcgtgtac caatcgtatt ggacatcttt gcagaacgtg accttgctcg tggtacattt
     3301 actgaatcag aaatccaaga attcgttgat gatttcgtta tgaaacttcg tacagttaaa
     3361 tttgctcgta caaaagctta tgaccaattg tactcaggtg acccaacctt tatcacaact
     3421 tctatggctg gtatgggtaa cgacggtcgt caccgtgtta ctaagatgga ctaccgtttc
     3481 ttgaacactc ttgacaacat cggtaactca ccagaaccaa acttgacagt tctttggact
     3541 gacaaattgc catacaactt ccgtcgctac tgtatgcaca tgagccacaa acactcttct
     3601 atccaatacg aaggtgtaac aacaatggct aaagacggat atggtgaaat gagctgtatc
     3661 tcatgctgtg tgtctccact tgatccagaa aatgaagaac aacgccacaa catccagtac
     3721 ttcggtgctc gtgtaaacgt tcttaaagcc cttcttactg gtttgaatgg tggttacgac
     3781 gatgttcaca aagactacaa agtatttgat atcgaaccaa tccgtgacga agttcttgaa
     3841 tttgaatcag ttaaatcgaa ctttgaaaaa tctcttgact ggttgactga cacttacgta
     3901 gatgccttga acatcatcca ctacatgact gataggtaca actacgaagc tgttcaaatg
     3961 gccttcttgc caactaaaca acgtgccaac atgggattcg gtatctgtgg atttgctaac
     4021 actgttgata cattgtcagc tatcaaatac gctacagtta aaccaatccg tgacgaagat
     4081 ggctacatct acgattacga aacaatcggt gactacccac gctggggtga agatgaccca
     4141 cgttcaaacg aattggcaga atggttgatc gaagcttaca caactcgtct acgtagccac
     4201 aaactataca aagacgcaga agctacagta tcacttttga caatcacatc taacgttgct
     4261 tactctaaac aaactggtaa ctcaccagtt cacaaaggtg tatacctcaa cgaagatggt
     4321 tctgtgaact tgtctaaact tgaattcttc tcaccaggtg ctaacccatc taacaaagct
     4381 aaaggtggtt ggttgcaaaa cttgaactca ctttctagcc ttgactttag ttatgcagct
     4441 gacggtatct cattgactac acaagtatca cctcgcgctc ttggtaagac tcgtgatgaa
     4501 caagttgata acttggtaac aatccttgat ggttacttcg aaaacggtgg acaacacgtt
     4561 aacttgaacg ttatggactt gaacgatgtt tacgaaaaaa tcatgtcagg cgaagacgtt
     4621 atcgtacgta tctctggata ctgtgtaaac actaaatacc tcactccaga acaaaaaact
     4681 gaattgacac aacgtgtctt ccacgaagtt ctttcaatgg atgacgcctt ggatgcattg
     4741 agctaatcaa gttcttgaat aataaaaagg aaccctcggt caaacgactg agggttttgt
     4801 gcttgggata gtatgagcaa ttccttcggc gcaatatgca atgtttt
//
LOCUS       DAVBGY010000088         4525 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVBGY010000088 DAVBGY010000000
VERSION     DAVBGY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.088"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            103..1128
                     /locus_tag="VQ620_001925"
     CDS             103..1128
                     /locus_tag="VQ620_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HET2764502.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            1118..2125
                     /locus_tag="VQ620_001926"
     CDS             1118..2125
                     /locus_tag="VQ620_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2764503.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLATFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            2125..3132
                     /locus_tag="VQ620_001927"
     CDS             2125..3132
                     /locus_tag="VQ620_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2764504.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            3132..3926
                     /locus_tag="VQ620_001928"
     CDS             3132..3926
                     /locus_tag="VQ620_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2764505.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
BASE COUNT         1649 a          650 c          757 g         1469 t
ORIGIN      
        1 tccttttttg aaaaaattat attattaaat ttgtaactgt atctattgac aatgataatt
       61 attattgata caatagactt gaaatatgtt taaggagttt ttatgaaaaa caaatttttt
      121 ctaatagcta ttttagctat gtgtatagtt tttagcgctt gttcttctaa ttctgttaaa
      181 aatgaagaaa atacttctaa agagcatgcg cctgataaaa tagttttaga tcatgctttc
      241 ggtcaaacta tattagataa aaaacctgaa agagttgcaa ctattgcttg gggaaatcat
      301 gatgtagcat tagctttagg aatagttcct gttggatttt caaaagcaaa ttacggtgta
      361 agtgctgata aaggagtttt accatggaca gaagaaaaaa tcaaagaact aaatggtaaa
      421 gctaacctat ttgacgattt ggatggactt aactttgaag caatatcaaa ttctaaacca
      481 gatgttatct tagcaggtta ttctggtata actaaagaag attatgacac tctatcaaaa
      541 attgctcctg tagcagcata caaatctaaa ccttggcaaa ctttatggag agatatgatt
      601 aaaattgatt caaaagcctt aggtatggaa aaagaaggtg atgagttaat caaaaatact
      661 gaagctcgta tatccaaaga attagaaaaa catccagaaa tcaaaggaaa aatcaaagga
      721 aaaaaagtat tatttactat gattaatgct gcagatacat caaaattctg gatttatact
      781 agcaaagatc caagagcaaa ttatttaaca gatttaggtc tagttttccc tgaatcatta
      841 aaagaatttg agagtgaaga tagttttgca aaggaaattt ctgcagaaga agcaaataag
      901 ataaatgatg ctgatgtaat cataacttat ggtgatgata aaactcttga agctttacaa
      961 aaagatcctc ttttaggtaa aataaatgca attaaaaatg gtgccgttgc tgtaattcca
     1021 gataatacac cgttagcagc ctcatgcact ccaacaccac tttcaataaa ctatactatt
     1081 gaagaatacc taaatctttt aggaaatgca tgcaaaaatg cgaaataaaa aacaaataaa
     1141 cctaggcata atttttgtaa tctgcctagg tcttcttatt acaatatttt tgtcattaaa
     1201 gcttggaaca aaagaaatta atatcagaga ttttttagca acttttggaa tgggcaatac
     1261 aaatgatgat tttattaaat caattatata taaaagaata cctagaacta tttttgcaat
     1321 tttagcaggt tctagtcttg ccataagcgg tgtattgatg caatcagtta ctagaaaccc
     1381 aatagctgat ccaggtatac tcggtataaa cacaggagca agtcttagtg tagtaattgg
     1441 tctttctttt ttaggaattt catcaagcat aagccatata agttttgcaa tcattggtgg
     1501 cttagtaagt gcaatttttg tatacgcgat tgctgtaagc ggaaaagcag gccttacccc
     1561 tataaaacta gccttatcag gaacttgtgt tagtatggct ttaagcagtt ttgtaagttt
     1621 tttaatttta ccgaataata acgtcttaga caaatttaga ttttggcaaa taggtagcct
     1681 tggagcagct acattatctt ctatatctac actactacct tttataattt taggtcactt
     1741 gatagctata tttatttcat cagatttaaa cgctttagct atgggtgatg aaatggctgt
     1801 tggtcttgga gttaatgtta ataggataag atcacttgca ataattgcaa gtgtgctttt
     1861 atgttcaagt attactgcaa ttggtggacc tattggcttc gtaggtctta tagttcctca
     1921 cttttgtggc ttatttataa gcaaagatat acgcacaatg accatttctt catcttttat
     1981 aggtgcagag ctcttgctta tatgtgatat aatcggccgt atgttaggta aaccaggtga
     2041 aattgaagta gggataatta ctgcaataat cgggggtcca gtacttattt atgtaactat
     2101 gaaaaataga ggggttaata actaatgcaa aatttaatta taggtattca aaaaagaaaa
     2161 aatagaataa cactattttc ctcactattt ttattaataa taatcagtct atcatttttc
     2221 attttactta tcggagatga aagttattct ttttcaactt tgattaaagt cttaaatagt
     2281 gaaactgttc ctggagctag tttttcgatt atggaaatta gattaccaaa attattagca
     2341 ggaattatag ctggctggtc ttttggattg gcaggattta tctttcaaac tatgttaaga
     2401 aatcctcttg caagtcctga tataatcggt gtcacaagtt cttcatctat tgcagcggtc
     2461 ttttgcatat tggtattaaa aacaaatagt ttaactactg gaattatttc aataacttgt
     2521 ggactaacat catctttaat attattttta ctagctaaaa aagatggttt ttcagcagca
     2581 agactgataa tattaggtat tggttttcaa gctgtcacaa gagcaggcac ctcattttta
     2641 ttgttgaaag tagcaagata tgaattacaa gaagttatga gatggctcag tggctcttta
     2701 tcttttacaa agttagatga catacctctt gttctaatag taagtattat tgctactata
     2761 ttagttttat tttttaataa aagactagaa attattgaac ttggtgaaga aatagcaatc
     2821 ggacttggag caaatcccga gctttcaagg cttgttttaa ttttttgcgc tgtatcttta
     2881 actgcttttt ctacttcaat tacaggacca atagcttgta tatctttttt agctggtccc
     2941 atagccttaa atattggcaa gaaaagaagc ccaatattag ctggattggt tggaatttta
     3001 ctagttttgt tatcagacat attctctcaa aatattttac cagctagata tccagtaggt
     3061 gttgtaactg gcttgttagg ttcaccatac ttaatatact tactaataaa aatgaacagg
     3121 aggaatatat aatgaaaggt ctgtggtcaa ataatcttac ctgcggttat gatgaaaaaa
     3181 taatcttgga aaatataaat ataaaaatac ctgaagaaaa aatatcagtt attattgggt
     3241 caaatggttg tgggaaatca acactcatta aaaccttgtc tcgacttata aagccattag
     3301 agggagaagt attgcttgat aataaatcaa ttaattctta taaagaaaaa gatttagcaa
     3361 aacacatagc tatattacct caatctccaa taatccctga atcaataaca gtagctgatc
     3421 ttgtaagccg tggtcgtttc ccctacagaa agccttttaa gagtcttgga aaagatgacc
     3481 ttgaaataat aaacagatca atggttaagg ccaatgttga agatctagca aataacctag
     3541 ttgaagaact ttctgggggt caaaggcaaa gagtatggat agctctagcc ctagcccaag
     3601 atacaagtat cctactttta gatgagccaa ctacttactt ggatatctca tatcaaatag
     3661 aactattaga cctcttgact gatctaaacc aaaaatataa gacaaccatt tgcatgattt
     3721 tgcacgatat aaatctaaca gcaagatacg ctgattacct atttgcaatt aaagaaggta
     3781 aacttgttgc agagggaaag cctgaagata tactaaatga taaactagtt aaagatatct
     3841 ttaatcttga agcaaaaatt atacgtgacc ctatttccaa ttcgcctcta atgattccta
     3901 ttggcaagca ccatgttagc tcttaataat tgaatattgg aattcattta aaagtcgcaa
     3961 attttgaact gaccctcaaa agttaggcca aaaaactaac taaaggaggt cagtttttta
     4021 atggcaaata cagcacagaa tttaagataa aattagtaaa agaatatcat gaatgaaaaa
     4081 taggatatag agaataacag caatattaaa aagatcagga ctgataataa acaaaaagaa
     4141 agtacaacga ctagttcaaa agctaaaact tcaagtaaaa agttattcaa gaaaatctag
     4201 aaaatactca tcctacaaag gacaggtagg aaaaatatca gacaacaaaa taaaaagaaa
     4261 tatttattac cgaaaaaaag gggaaatcga gtcaagtacc aacaggctca tctattccaa
     4321 aaagatattt actcgatgga aaaactccta gaattagtgc tacaaatatt gacaatggta
     4381 tcttaggtta ctacgaagat atagatgata aaaattatag agtttttgaa aattttatat
     4441 ctgtttcatt tttaggagca gtcttttatc ataaatataa agcaagccta gatatgaaaa
     4501 ttcattgttt aaaattaaaa aataa
//
LOCUS       DAVBGY010000089         4523 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVBGY010000089 DAVBGY010000000
VERSION     DAVBGY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.089"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(1..419)
                     /locus_tag="VQ620_001929"
                     /pseudo
     CDS             complement(1..419)
                     /locus_tag="VQ620_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(495..929)
                     /locus_tag="VQ620_001930"
     CDS             complement(495..929)
                     /locus_tag="VQ620_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2764506.1"
                     /translation="MLLVLVSLVHSFGSVSIIAHTIHQKFNLKVPNYRQEEDWARMGL
                     PITRKEISNWHIKASQYYLESLYKLLREKLLEQPLLHADETSYRVLESDSHLTYYWTF
                     LSGKAENQAITLYHHDQRRSGLVVQEFLGDYSGYVHCDMLRQ"
     gene            complement(1005..1856)
                     /gene="rfbD"
                     /locus_tag="VQ620_001931"
                     /pseudo
     CDS             complement(1005..1856)
                     /gene="rfbD"
                     /locus_tag="VQ620_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
     gene            complement(1922..2971)
                     /gene="rfbB"
                     /locus_tag="VQ620_001932"
     CDS             complement(1922..2971)
                     /gene="rfbB"
                     /locus_tag="VQ620_001932"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET2764507.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLVAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGIYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQATD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            complement(2981..3577)
                     /locus_tag="VQ620_001933"
     CDS             complement(2981..3577)
                     /locus_tag="VQ620_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HET2764508.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     VLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENLEEADVSEADKHHPLLKDVKPLKKEDLE"
     gene            complement(3578..4435)
                     /gene="rfbA"
                     /locus_tag="VQ620_001934"
     CDS             complement(3578..4435)
                     /gene="rfbA"
                     /locus_tag="VQ620_001934"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET2764509.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGIKLSYAEQPSTDGLAIGEEFISDDSVALIL
                     GDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEKPEYP
                     RSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRGFAWL
                     NTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKNEYGQ
                     YLLRLIGEA"
BASE COUNT         1259 a         1056 c          826 g         1382 t
ORIGIN      
        1 ttactccgtc ccataaccaa tgatttaatg gcgcattcag ctagattatt ggaaaggacc
       61 agatgtccgt ctttcaaaat agtcttaaag gtttcttcat acttgaggct gtattcaatt
      121 gcccttccta gttttgaacc tgctaaaact gactgacgcc ggcaccaagc aaagaagtct
      181 tccattaggg gctggagatg ttcttgacgt ttctgtagtc gttcatcagc tggcaaagcc
      241 tcccagtctc tttccaggga aaataactga tcacaataag ctaaaccttt agctcctaag
      301 gatgatttat ccgtttgctt ggggggcgct tcaaaaaact tccttctcac atgtgcccaa
      361 catccaacaa gttcagcttc ttccagttga cgataagctt ctagcggttc gcagtttacc
      421 aagcttagcg tcgccttgct cctaaacctt ggactgctat cgcataggca gaactagagg
      481 actaaagtcc taagttactg ccgcaacata tcacaatgca catagccaga ataatctcct
      541 aggaattctt gtactactaa accactccga cgctgatcat ggtggtacag cgtgattgct
      601 tgattctcag ctttcccaga caaaaaagtc caatagtagg tcagatggct atcactctct
      661 agaacccgat aagaggtttc atccgcatga agaagaggtt gttctaacaa cttttctcgt
      721 aaaagcttat aaagggactc taaatagtat tgacttgcct tgatatgcca attagagatt
      781 tccttacgtg tgattggtaa acccatccta gcccaatctt cttcttggcg ataattgggt
      841 accttcagat taaacttctg atggatggtg tgagcgataa tagaaactga gccaaagcta
      901 tgcaccaaag agaccagcac caacaacaag ataattgtac atcttaagct cctttggagt
      961 tcattatctg cctcttttat tagactagaa aatcattcta ctacttatct cacttcttgt
     1021 ttgtaaaatt cttgcaatgc atcttgccaa gttggaataa caaatccagt agctttggct
     1081 ttggccaggc tcatcgttga gtttagcgga cgttgagctt tggctggaaa ttgactggaa
     1141 tctactggct tgacttcgac atctgtatct ttcaaaattt caactgcaaa atcataccat
     1201 gttgtgtctt ctgtcgcatc atttgacaaa tgataataac caaattcctt acgattttca
     1261 gctaggtaga tcatgaattc agccaaggta cgagtccaag tcggacgacc gtactggtca
     1321 tttacaactg ttaaagtctt atgagttttc gcaagatttt gcatggtaaa aacgaagttt
     1381 ttgccataat ttccaaatac ccaggcagta cggataatat agaaattaga cacatgcttc
     1441 tcaactaact cttcccccat acgcttagtg cgtccatatt ctgtctgtgg atctggtcgg
     1501 tcatcaactt cccactcttg tccaactggt ttcttaccgt caaagaccta gtccgtagaa
     1561 atataaacta gagttgcacc atgcttttca gatgcttttg cgacattttt tgtccccatc
     1621 acattgatgg cgaagtccaa ctcttttcct tcatcctctg ctgcatcaac agcggtgtag
     1681 gctgcacaat ggtagactaa agtcggtttc acctcttcaa aaactttctc aaccatttct
     1741 tcattggtaa tgtccatctc agccacatct actgccacgt attcttcatt acgttcatcc
     1801 aataaatagc gaagttccgt tcctaattgg ccatttgccc ctgtaattaa aatcatctct
     1861 agacctttct aatatgtaac tcttcccaat atagcaataa aaagcagcga tttcctgctt
     1921 tttatactgt aataatctcc tgagtcttag cataattggc ttcaacagct tctttttctg
     1981 ctttccacca ttcttggtta tctgtatacc acttgattgt tgccttgagc ccagcttcaa
     2041 agttggtaaa ttcaggtttc caccccaact catcacggag cttgctggca tcaatcgcat
     2101 agcgaaggtc atgtcctgca cggtcagtca catgatcata ggcatccgta gcttgtccca
     2161 tttccttaag gataagttcc aaaacttcct tattgttctt ctcaccatca gccccaatca
     2221 agtaggtttc accgatttgc ccttttgtca agattgtcca aactcctgaa gaatggtcat
     2281 tggtatgaat ccagtcacga acgttcttac cttcaccgta aagttttggc ttaattccac
     2341 ttaggatgtt agtaatctga cgtgggatga atttttcgat atgttgataa ggaccgtagt
     2401 tatttgaaca gttggaaatc gttgccttga ctccaaaaga acgcacccag gctttgacaa
     2461 tcaagtctga tgcggccttg gttgatgagt acggagagct tgggttgtat tttgtttcag
     2521 cagtaaattt ctcacctggt ccttcaccat gacctggcaa atcttctcgt aaagggagat
     2581 ccccataaac ttcatctgtc gatacatggt ggaagcgaat atcatactta cgagcagctt
     2641 ctaaaagagt atagattcca atgaagttag tatgaataaa tggcgatgga tcattgagcg
     2701 aattatcatt gtggctttcc gctgcataat gaacgatagc atctgcttga gcaaccaact
     2761 tgtctaccaa ctccgcatca gcaatgtcac caacaactaa ctcaacacga ttgcctaaaa
     2821 tttcctcaat attcgcgcgg tttccagcat aagtcaactt atctaggact gtcacgtgaa
     2881 catctggaaa gttctcgtaa acataatgga caaagttaga accgataaag ccagctccac
     2941 ctgtcacgat aatatttttg tattcagtca tattctttcc ttattccaaa tcttcttttt
     3001 tcaaaggctt cacgtcctta agtaggggat gatgtttgtc tgcttcagat acgtctgctt
     3061 cttccagatt ttcccattca ataccaaggc ttggatcagc gtagttcaca aaggcatact
     3121 tgggtttgag ttcaagagcc cagtaatcat tgaccagata gctatatgac actgtatctg
     3181 ataaaacttg gaagccatta gctacgcctc gaggaacaaa gattcccttg cttgcatcaa
     3241 ttactgtctg ataggtattc ccaaaggttt caccctcgcg tagatcaacc caagaaccca
     3301 gaactttccc tccatctgct acagagatgt acttatccca aggctctgcg tggaggcctc
     3361 gaaggacatt tttacgtgag aaggatacat tgttttgcaa ttttccttct gcaaagaaag
     3421 actctggaaa tccaagtgga agcacttttt ccttttggaa attttcttta aaccagccac
     3481 gattatctcc atgaacgggg atatcaaact ccaacatgcc tggaatagct tcaaccttgc
     3541 gtgccgcaag cgtcttaccg aaaaaattat ctgtcatcta tgcttctcca atcaaacgga
     3601 gcagatactg tccgtattca tttttcttaa gtggttgggc taaggccaat acatcttctc
     3661 gactgatata gcccatacgg taagcaattt cttctaagtt tgctacctga acattttgca
     3721 tccgttgcac tgtttcgatg tactgtgaag cctctagtaa actttcatga gttccagtat
     3781 tcagccaagc aaagccacgt cccataagct caacagacaa atcaccacga tctaggtaag
     3841 ccttgtttac atctgtaatt tccagttcac cacgagggct tggtttaata ctcttggcaa
     3901 tttcgacaac atcgttatca tagaaataca gtcctgtcac tgcatagttg gagcgaggat
     3961 actccggttt ttcttctatg gaaatagcat tcatgtctgt atcaaactca accacaccaa
     4021 aacgctctgg atccttcact tggtagccaa aaacagtcgc acctttctct ttcttggctg
     4081 ctttttgaag cattgtgctc agaccaggac catggtagat attgtcgcct aaaatcaagg
     4141 caacgctatc gtcagaaata aactcttccc caattgccaa accatctgta cttggttgct
     4201 ctgcataaga aagtttgatc ccaaactcag atccgtcttg aagaagctct tgaaatcgat
     4261 gtaaatcctg tggagtggaa ataatcaaaa tatccctaat cccagccaac atcaatgttg
     4321 aaagtgggta gtaaatcatc ggtttatcat aaaccggcat aagttgtttt gacgcagcgc
     4381 gagtcaaagg atataaacgt gtccccgaac cacctgctag aataatacct ttcatagtag
     4441 ggtacctttc tttgttattt tagagtcaat tactattaat tgtgtactgc tctaaaaaga
     4501 aaacaatcat cccatgtttt ata
//
LOCUS       DAVBGY010000090         4491 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVBGY010000090 DAVBGY010000000
VERSION     DAVBGY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.090"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(56..1066)
                     /locus_tag="VQ620_001935"
     CDS             complement(56..1066)
                     /locus_tag="VQ620_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HET2764510.1"
                     /translation="MSFLSKNGAGILACLLISIVSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            1215..2384
                     /locus_tag="VQ620_001936"
     CDS             1215..2384
                     /locus_tag="VQ620_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2764511.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2381..3151
                     /gene="recO"
                     /locus_tag="VQ620_001937"
     CDS             2381..3151
                     /gene="recO"
                     /locus_tag="VQ620_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET2764512.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3148..4140
                     /gene="plsX"
                     /locus_tag="VQ620_001938"
     CDS             3148..4140
                     /gene="plsX"
                     /locus_tag="VQ620_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET2764513.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4146..4379
                     /locus_tag="VQ620_001939"
     CDS             4146..4379
                     /locus_tag="VQ620_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET2764514.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQSVGDVVKIIQGK"
BASE COUNT         1309 a          852 c         1153 g         1177 t
ORIGIN      
        1 aacatatata cggcaaggcg acgttgacgt ggtttgaaga gattttcgaa gagtgttaga
       61 aaatgccgat aagggtctgc ataccaagag tggtgaggat gatggcaatc cagcagatgg
      121 ctccaagaag aatggatttt ccactggatt tgaccatagc gaccagattg gttttgagac
      181 cgatagcact catggccatg acaataagga atttagagag ttctttgaga ggggtaaaga
      241 aactactgga cacacctaga gaggtgagta gtgtagtgag gagagaggca aggataaagt
      301 aaaggataaa aagtgggaag acttttttca gttgtaggct ttgcttgttt tcttgttggc
      361 gactttgcca gtaggagaga aagagcgtga tagggataat agccaaagta cgtgtgagtt
      421 taacaatggt tgcagactcg agggtattgc tttggtaaag actgtcccaa gcgctggcgg
      481 cagccgttac agaggaagtg tcgttgaccg cagtccctgc aaagagggcg aagccttcat
      541 tggatagatg aagccaggtg ccgagggttg gaaagatgag cgcagccaag acattgaaga
      601 aaaagataac ggaaatggct tgggctactt ccttttcctt agcatcaata acgggcgctg
      661 tcgctgcaat ggcagaaccc ccacagatag aagaacctac tccaaccaag gtagccagtt
      721 ttgtatccag tgcaaagaaa cgctggaaga ggtaggcaat aatcagagct attgagatag
      781 tggacaggat gacagggagt gaagattggc caactgcgaa gacctgcgag atattgagac
      841 caaaaccaag caagacaacg gcatattgga gcaacttctt ggaactaaag gtcaaaccag
      901 catccagttg tttataggac gagagaaagg gatgtaggag catgcctatg aaaatggcaa
      961 aaacgggcgc gccaatcaca gggaagaatc ctcctaagta ccaagatacg atagaaatga
     1021 gaaggcaggc caagatacct gctccatttt ttgatagaaa tgacataaaa acctccaaat
     1081 aaaatctatt accattatag acctataaac aggaaaagta aaatagaaag tggaaagcta
     1141 ttctctaatg tatttttgcg gtcggggggc ttttgtagta taatagagat acgttttgaa
     1201 agtaggaggt atctatggac ttaactaagc gctttaataa acagttagat aaaattcaag
     1261 tttcgttgat tcgtcagttt gaccaggtta tttcggagat tcctggggtc ttgcgtttga
     1321 ccttggggga acctgatttt acaacgccag accatgtcaa ggaggcggcc aagcgagcga
     1381 ttgatcagaa ccaatcctac tatacaggga tgagtggtct gctgactcta cgtcaggcag
     1441 ccagtgactt tgttaaggaa aagtaccaac tggactatgc tcctgaaaat gaaattttgg
     1501 ttacaattgg ggcgacagag gctttatctg cgactttgac ggctattttg gaagagggag
     1561 acaaggtact tttgccagct cctgcttatc caggctatga accgattgtt aacttagttg
     1621 gggcagaaat tgttgagatt gatacgactg aaaatggttt tgtcttgact cctgagatgt
     1681 tggagaaggc cattttggag cagggtgata agctcaaggc ggttattctc aactatccag
     1741 ccaatccgac aggaattacc tacagtcgag agcagttaga ggccttggca gctgttttac
     1801 gcaagtacga aatttttgtt gtctgtgatg aggtttactc agaattgacc tacacaggcg
     1861 aagcccatgt gtctctagga acgatgttga gagaccaggc tattattatc aatggtttgt
     1921 ctaaatcgca tgccatgaca ggttggcgtt tggggctgat tttcgctcct gcggccttca
     1981 cagcccagtt aatcaagagt caccaatact tggtcactgc cgcaaatacc atggcgcaac
     2041 atgctgcggt agaagccttg acggctggta aaaatgatgc ggagcccatg aagaaggaat
     2101 atatccaacg tcgggactat atcatcgaaa aaatgactgc tcttggtttt gagattatca
     2161 aaccagacgg tgccttctat atttttgcta aaattccagc gggctacaat caagactcct
     2221 ttgcttttct gaaggatttt gctcagaaga aggccgttgc ctttatccct ggtgcagcct
     2281 ttggacgtta cggggaaggc tacgtccgcc tatcttatgc agccagcatg gagactatca
     2341 aagaagccat gaaacgactt gaggagtaca tgagagaagc atgattcagt ctatcacgag
     2401 tcaaggcttg gtgctttaca atcgcaattt tcgtgaggat gacaagctcg tcaaaatttt
     2461 tacagagcag gttggcaaac gcatgttttt tgtcaaacac gctggtcagt ctaagctggc
     2521 gcctgttatt cagcccttgg tgctggcacg atttctcttg cgaatcaatg atgacggact
     2581 cagttacatc gaagactatc atgaggtcat gacttttccc aagattaata gtgacctctt
     2641 tgtcatggcc tatgcgacct atgtggcagc tcttgcagat gctagtttgc aggacaatca
     2701 gcaggatgct cccttgtttg cttttttgca aaagactttg gagttgatgg aagcaggctt
     2761 ggattatcag gttttgacca atatttttga aattcaaatt ttgactcgat ttggaatcag
     2821 cctcaatttt aatgagtgtg tcttctgcca tcgggttggt caggcttttg acttttcttt
     2881 caaatatgga gcctgcctct gtccagagca ttatcatgag gataagagac gttgtcatct
     2941 caatcccaat atcccctatc tgctcaatca atttcaagct attgattttg agactttgga
     3001 gaccatttcg ctcaagcctg gaatcaagca agagctacgc caatttatgg atcaattata
     3061 tgaagagtac gttgggattc acctaaaatc aaagaaattt attgattccc tagcagactg
     3121 gggacaatta ctaaaagagg aaaagaaatg aaaaaaatcg cagtagatgc catggggggc
     3181 gattacgcac ctcaggccat tgttgagggt gtcaatcaag ccctatctga cttttcagat
     3241 atcgaggttc aactttacgg agatgaagct aaaatcaagc aatatctgac agcgacagag
     3301 cgcgtcagca ttatccatac ggatgagaag attgattcgg atgatgaacc tacgagagct
     3361 attaggaata agaaaaatgc cagtatggta ttggcagcca aggctgtcaa agatggtgaa
     3421 gcagacgctg tcctttcggc tgggaataca ggtgccttgt tggcagcagg attcttcatc
     3481 gtgggtcgta tcaagaatat cgaccgtcct ggactcatgt ctaccttgcc taccgttgat
     3541 ggaaaaggtt ttgacatgct agaccttggt gccaatgcag aaaatacagc ccagcacctc
     3601 catcaatatg cggttctagg ttccttctat gctaaaaatg tccgtggcat tgcgcaacca
     3661 cgcgttggtt tgctcaacaa cggaacagag agtagcaagg gcgacccgct tcgtaaggaa
     3721 acttatgaat tactggcggc tgatgaaagt ttgaacttta tcggaaacgt ggaagcgcgt
     3781 gatttgatga atggcgttgc agatgttgtt gtggcagatg gtttcacggg aaacgctgtg
     3841 ctcaaatcca tcgaagggac agctatggga atcatgggct tgctcaagac agctattaca
     3901 ggtggtggtc ttcgagcgaa actaggtgcc ctccttctca aggacagcct cagaggtttg
     3961 aaaaaacagc tcaattattc agatgttggt ggagcggtct tgtttggtgt taaggcacct
     4021 gttgtcaaga ctcatggctc aagcgatgcc aaggctgttt atagtacgat tcgtcagatc
     4081 cgtaccatgc tagaaacaga cgtggttgcc cagactgcgc gtgaattttc aggagaataa
     4141 aagagatgac agaaaaagaa atttttgacc gtattgtgac cattatccaa gagcgacagg
     4201 gagaggactt tgtcgtgaca gaatccttga gtctgaaaga cgatttggat gcggattctg
     4261 ttgacttgat ggagtttatc ttgactctgg aagatgaatt tagtatcgaa atcagcgatg
     4321 aggaaattga ccaactccaa agtgtaggag atgtggttaa aatcattcaa ggaaaatagc
     4381 aatcggagtt ccaagtcaac ggaagtagat ggtttttaga aatgagaaat atcggacaag
     4441 ctggtaaaat cttggctgac agtggttatc aagggctcat gaagatatat c
//
LOCUS       DAVBGY010000091         4485 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVBGY010000091 DAVBGY010000000
VERSION     DAVBGY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.091"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(56..289)
                     /locus_tag="VQ620_001940"
     CDS             complement(56..289)
                     /locus_tag="VQ620_001940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764515.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            complement(282..398)
                     /locus_tag="VQ620_001941"
     CDS             complement(282..398)
                     /locus_tag="VQ620_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET2764516.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            complement(613..966)
                     /locus_tag="VQ620_001942"
     CDS             complement(613..966)
                     /locus_tag="VQ620_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET2764517.1"
                     /translation="MILRHLGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAKNVTIL
                     EGSNIHVLPSMPYSAQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQV
                     NQNLEKIRQREGDTH"
     gene            1219..3852
                     /gene="polA"
                     /locus_tag="VQ620_001943"
     CDS             1219..3852
                     /gene="polA"
                     /locus_tag="VQ620_001943"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET2764518.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            3937..4374
                     /locus_tag="VQ620_001944"
     CDS             3937..4374
                     /locus_tag="VQ620_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET2764519.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
BASE COUNT         1218 a          829 c         1086 g         1352 t
ORIGIN      
        1 taggctccat aatatccata ggggatttac ccactatgga tattatggag ccattttaca
       61 acagtgcctc aaattcagat actgacatac ctaattgctg gctggcaacc tcagaagtca
      121 gaagaccttg gcgtactatg tctaggaagg caaaaagttt cctttctgcc cttccttcaa
      181 ctttcccacg ctctagtcct tgttcaaggc cttcagccct agcagtttcc aaggcatagt
      241 cctgtgctaa caagacttgt tcttctcgca tatgtagttg actaaacatt ttcctgtcct
      301 cctcggacca gctcttgtag tccagcagtt gatttgcttg ggtaatggct cgttgcggtt
      361 gctgggtaaa gggcttgttg ccgaaaaact ccaaccatgg cttgcgaacc ttgtctttgc
      421 tggtttctct gtatttttta attccaagaa tgccatctta accagatggt tttcctgtcc
      481 attgttggta atcgccaata cctcacctgt tgtgtcttcg cgcatactaa agctatgaaa
      541 agccaggtca tctgagaaat aattactatc cacgatagca atgacgtaaa caggagcgat
      601 gtgtttgtag atctagtgag tatcaccttc tcgctgacga attttttcaa gattttgatt
      661 aacctgactg cacaggtaag cccacaagtg attgatgaaa aaattctgat gatggacttg
      721 aatctcaata attacttgag taccgttatc caactccgcc aagacgtcta tactggtata
      781 aaaatcctgc gctgagtagg gcatggaagg caagacatga atgttacttc cctccaaaat
      841 agtcacattt ttggctggca agtccagcat atcgcggata aattgacaag tgatttctgg
      901 gttgctgaaa attttcttag caaccaagtc attggtcggg ctaattccca gatgtctgag
      961 aatcatcctt ttcctccttc tatagtgaat tgaaataaga tgtgaacaac tctatcagga
     1021 aagtcaaatt aatttataga aatattttag cagtcaaggt gtactattat agattcaata
     1081 tactatatta tagcacattt ttaaatctag gtttactaaa ttctctggct ctatctattt
     1141 attcggaaaa agtttgaaaa atacttgctc tagctcttcc caatggtatt ttttgattct
     1201 ttcctttata atgggtgtat ggataagaaa aaattattat tgattgatgg gtcttctgta
     1261 gcttttcggg cgttttttgc gctgtatcag cagttggacc gttttaagaa tgcggctggt
     1321 ttgcatacca atgcgattta tggttttcag ttgatgttga gtcatttgtt ggagcgggtt
     1381 gagccgagtc atattttggt ggcttttgat gcgggaaaga cgaccttccg gacagagatg
     1441 tatgcggact ataagggtgg tcgggccaag actcctgatg agtttcgtga gcaatttcct
     1501 ttcattcgtg agttgctgga tcatatgggg attcgtcact atgagctggc tcagtatgag
     1561 gcggatgaca tcattgggac gctggataag ctagcagagc aggatggttt tgatattacc
     1621 attgtcagtg gggacaagga tttgattcag ctgacggatg agcatacggt ggttgaaatt
     1681 tccaagaaag gtgtggctga gtttgaggcc tttacgccag attacctcat ggaagaaatg
     1741 ggcctcacac cagctcagtt tatcgatctc aaggcgctca tgggtgataa gtcggataat
     1801 atccctgggg tgaccaaagt cggtgaaaag acgggtatta agctcttgct ggagcatggt
     1861 tcgcttgagg ggatttatga aaatattgat ggaatgaaga cttctaagat gaaggaaaat
     1921 ctcatcaatg acaaggaaca ggcctttttg tctaaaacac tagcgaccat tgataccaag
     1981 gcaccgattg cgattggttt agaggacttg gtctatagtg gtccagatgt tgaaaatctt
     2041 gggaaatttt acgatgagat gggattcaaa cagctcaagc aggctttaaa tgtgtcgtca
     2101 gctgatgtgg ctgagggttt ggattttact attgttgacc aaatcagtca agatatgctg
     2161 agtgaagagt ctatcttcca ctttgagctt tttggtgaga attatcatac ggataatttg
     2221 gttggatttg cctggtcttg tggggataag ctctatgcca cagacaagct tgagctgttg
     2281 caagacccga ttttcaagga tttcttagaa aaaacatctc tgagagttta tgactttaag
     2341 aaggttaaag ttcttttgca acgttttggt gtggatttgc aggcgcctgc ttttgacatc
     2401 cgtttggcta aatacctcct ttcgactgtg gaggacaatg aaattgcgac catcgctagt
     2461 ctttatggtc agacttactt ggttgatgat gaaactttct acggtaaggg tgttaaaaag
     2521 gccattcctg aacgtgagaa attcttggaa cacttagctt gtaaacttgc tgttttggta
     2581 gaaacagagc ctattttact tgaaaaactc agcgaaaatg ggcaattaga gcttctttat
     2641 gatatggagc aacctctggc ttttgttctt gccaagatgg aaattgctgg gattgtggtc
     2701 aagaaagaga ccttgcttga gatgcaggct gaaaatgagc ttgtcattga aaaactgact
     2761 caagagattt acgagctggc tggtgaggag tttaatgtca actcgcctaa gcagttgggc
     2821 gtgcttctct ttgagaaatt gggacttcct ctagaataca ctaagaaaac caagacaggt
     2881 tattcgacag cagtggatgt gttggagcgt ctcgctccta ttgctccgat tgttaagaaa
     2941 atcctggatt accgtcaaat tgctaagatt caatctactt atgtaattgg cttgcaggac
     3001 tggattttgg ctgatggaaa gattcatact cgctatatgc aggatttgac ccagaccggg
     3061 cgtttgtcta gtgtggatcc aaacttgcaa aatattcctg cccgattgga acaagggcgc
     3121 ttgattcgga aggcttttgt gccagagtgg gaggatagtg tgctactcag ctctgactat
     3181 tcacagattg aattgcgcgt tttggcgcat atttctaagg atgagcactt gattaaggcc
     3241 ttccaagagg gggcagatat ccatacttcg acagccatgc gggtctttgg cattgagcgt
     3301 cctgataatg tgactgcaaa tgatcgtcgc aatgccaagg cagttaactt tggagtggtt
     3361 tatgggattt cagactttgg cttgtctaat aatttgggaa ttagtcgtaa ggaagccaaa
     3421 gcctacattg atacctactt tgaacgtttt ccaggtatta aaaactacat ggatgaagtg
     3481 gtgcgggagg cgcgtgataa gggctatgta gagaccctct ttaagcgtcg ccgtgagttg
     3541 ccagatatca attcgcgcaa cttcaatatt cgtggttttg cggagcgaac tgctatcaac
     3601 tcacctatcc agggttcggc agcagatatt ctcaagattg ccatgattca gctggataaa
     3661 gccttggttg caggtggtta tcagactaag atgctgttac aagtgcacga tgaaatcgtc
     3721 cttgaagtgc ctaaatctga attggtagag atgaaaaaat tggtgaaaca aaccatggaa
     3781 gaagccattc aactcagtgt tcctcttatc gcagatgaga atgaaggggc aacctggtac
     3841 gaggctaaat aaaaaggggg ctagtcctcc ttttttgtag tagaattctg caaacctttt
     3901 caaaatgtgc tatactgatg aaaaaggagg ttttctatga gtcaagaatt tatcaatcca
     3961 agtgatggcg tgattcgtca gtatctcgca acgagtaaaa cccttgctgt ggtgggtttg
     4021 tctgaccgtg aagaaacaac cagcaatcga gtgacaaagg aaatgcaggc tcggggctat
     4081 aaaatcatcc cagtcaatcc caaggcggca ggtggcgaaa tcttgggtga aaaggcttat
     4141 gctagcctcg ctgaaattcc ttttcctgta gatattgtca atgtttatcg tcgcagtgaa
     4201 tttctgcccg atgtggcgcg tgattttctc aaagctgatg ccaagatttt ttgggcacag
     4261 ctaggacttg aaagtttaga agcgaaagag attttgcgtg atggtggatg tgatgatatc
     4321 gtgatgaatc gctgtatcaa gagagaacat actcgcttga ttgaggaagc ataagaaaaa
     4381 ggtagctgat gggctacctt ttgtgttata ctcaatgaaa atcaaagagc aaactaggaa
     4441 actagtcgcg ggctgctcaa aacactgttt tgaggttgta gatac
//
LOCUS       DAVBGY010000092         4304 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVBGY010000092 DAVBGY010000000
VERSION     DAVBGY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.092"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(<1..617)
                     /locus_tag="VQ620_001945"
     CDS             complement(<1..617)
                     /locus_tag="VQ620_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HET2764520.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLEN"
     gene            complement(726..1244)
                     /locus_tag="VQ620_001946"
     CDS             complement(726..1244)
                     /locus_tag="VQ620_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HET2764521.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1471..2340)
                     /gene="rsmI"
                     /locus_tag="VQ620_001947"
     CDS             complement(1471..2340)
                     /gene="rsmI"
                     /locus_tag="VQ620_001947"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET2764522.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGTSAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2343..2660)
                     /gene="yabA"
                     /locus_tag="VQ620_001948"
     CDS             complement(2343..2660)
                     /gene="yabA"
                     /locus_tag="VQ620_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HET2764523.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(2700..3590)
                     /locus_tag="VQ620_001949"
     CDS             complement(2700..3590)
                     /locus_tag="VQ620_001949"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET2764524.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(3587..4225)
                     /locus_tag="VQ620_001950"
     CDS             complement(3587..4225)
                     /locus_tag="VQ620_001950"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET2764525.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
BASE COUNT         1194 a          979 c          685 g         1446 t
ORIGIN      
        1 taattctcaa gctgtgagtt cattatgatt tgtaaatccg atatatcttc agcagctaaa
       61 aacacatttt gaaatgttcc aagtccgttt tcaccaaaac tatattctct cttactatac
      121 ttactcttat tattctctat atagttagag tcaaggttct taacctcttg aagttcattt
      181 tctttacttc tgaggttaaa atcttttact tctgaagtta agttttttga cttctgtaag
      241 tcattttctt ttacctgaaa tatactcata aagtctttaa cataaatgat gttcggctta
      301 ccaagtccaa gccttactct ttcgatcagt cctattcctt tttttacatc aagctcatct
      361 aatgttttta tggcagttgg ctttgagata tttcttcttt tcataatttc ttcgacagta
      421 aaatagataa atactctgcc ttccttgtct atccagttat tcttaaatga cattcctgtc
      481 tgtttaagaa gtatggaata aaggataatt gcttctgctg ataagccccg aaattcttct
      541 ctatcaacta atatctccgg tacttttaag aagttaaatc tttccgcttc tctgttataa
      601 aaatattcaa agtccatgca agatcacctc ccttcttaaa aatttgcaaa gaaaaagacg
      661 atagtttttc ttctatcgcc tttggtaacc atttttatga aattttttaa agttaaaaat
      721 ataatttata atactatctt cttccatttt ctgattttag ttctgaaagt tccaaaaggt
      781 gcaaccgtat ttacatgaat aaacttatac acttcccaag tcgctgtttt agtcgcttca
      841 tcagcccact ttctcatatg cggttcaaat aattcttcct cactcaacga atcaatcatt
      901 gcagagatag aattaatatt ttcatttaat tttgctttca actcttgcag agataaatga
      961 gcgtaggtat ctgtgaacca ctgatataat tcaccaagtt gattccattt aaatttatcc
     1021 gatggtgttt ttacttgaag cccctttctt tcatcttctt cccatttaag aaccaagttg
     1081 gtccaaccaa cctgataagc aaggttttct gctggagttc tgtcaacttc atcagctctc
     1141 ttatctttta aattttctgg aatattatca aattctaaaa tatatttctc aaatgttttc
     1201 tctatctcag cttttagttc ttctttattt tcgtatgttc gcaaaaaatc accttctttc
     1261 tgatttatca atcctttgat ttaaaatgac cttatttttc tatttatacc ttctaagaac
     1321 gctataactg ctgctccaaa catcggtatt ccgtatgggc ttaataaaaa acttaatacc
     1381 aaagcttcta taccaagtga tacatcttta tattatcatc actaattaag ttaaacagaa
     1441 acagacagaa ttattacatc ctgtctccct ttattgtttt tcttcccaat cgtggtaggc
     1501 agcgtagagt tgactcttat tccactggta aatcttagct acttccttaa tagcttgatt
     1561 tttcttcatg ccttgctgga tacgggcttg gatttctaag aacaagtcct cctcgtcttt
     1621 ttcctccaca tcctgactgg caccttcaac aataagaagg cattcgccct tgagtggcgt
     1681 ttcagcgatg ctttccagta attcagaaat tgtacctcgt tggtattctt catagatttt
     1741 ggtcaattcc ctgaccaaaa caaccgagcg gtcaccgtag acttctaaca tattttccaa
     1801 cgtgtctgct acacgatgag gtgattcata aaaaatctgt gtttcaggat aatctttttt
     1861 agagccaaaa aattgcttct gttgacctga ttttctcggt aaaaaaccgt aaaagatatg
     1921 tggctgtggc gctaaaccac tggcaatcaa ggcagaaatt cctgcagagg tacctggaac
     1981 agtcacaact gcaatttctt cctcaatagc tgccttaact aaatcatgac cagggtctga
     2041 aatgctaggc aaaccggcat cagagacctg agcaatactt tgccctgctt tcaagaaacc
     2101 aatcaaatca ggaatttttt ccttggcatt gtgctcatga aaactgatct gcttggtgga
     2161 aatgtcaaaa tgcttgagca aaagccctgt attgcgcgta tcctcagcag caatccagtc
     2221 cacttctttc aaggtctgga tagcacgaaa agtcatatca tctagattgc caatcggcgt
     2281 tgccactaga tacagcttgc catagggaga ctgcccctta aaactttttt gaatttgcat
     2341 gcttactccc tgtataacaa ctcatcacag aacatacatt cttcgtcctg ctctcgacgt
     2401 tgtccataaa aatcattaca tacgtgaaat ccatcacggt aaatacgacg gacactttcg
     2461 cgaacatgct tggccttgac gggagcgtct gcttccacct cacccaagcg ttctcgcaac
     2521 ttactatttt ccaagcgaag agctgtattt tcttctacca agctcttgag atttttcttg
     2581 atggcttcca catcggctaa agttaccaat aattgttggg aaaaatcatc cagcgcgtca
     2641 aataattctt ttttgtccat aaaacagccc tttcctttct ttatcattca tttttgagtt
     2701 tatatttctt tcaagaccag atattccatg gcattttgaa agctgacatt agcttgccac
     2761 atttttctag cttctagtaa atcttgtaga atcactctta ctcttacctg caagaggtcc
     2821 tgcccacaga gaacttcaag aatccgtaaa acctgatcct gtttttcctt atcatctgcc
     2881 aagttggcta atttggcaac ctgtagataa ctttcttttt tcttagctac taaccaagtc
     2941 agcaggcgtt cactttcatc gaccaaggtc caaaaacttg cctgattagc caacttttct
     3001 gcttcagctc gcgattgact aaacttagct aaaagagtcg cttttttctt aacaagtccc
     3061 atttgttcta agagtaagat aagtttttct tcttgctttt taaagtggaa gatctgagtc
     3121 cgacttcgga ttgtcggtaa catcttttcc tcatcgctag tcaagaagaa aatataaact
     3181 tcactctggg gttcttcgat gaccttgagc agagaattgg ctgcgttggg atgcatttta
     3241 tccgcttgct cgatgataaa gacctgttgc tggctttcaa tccctgcttg agaaaactgt
     3301 cccaccaatt cccgaatacg ttctgtcttg atgacctgat tgactggctt aatcaaggtg
     3361 acatctggaa actcttcctg ttcaatcagc ttgcaacttc ggcatttctc acatggtaag
     3421 acgccaactt tatccgtaca aaagaggctc ttagctaaaa attgcgccat ttccaagctt
     3481 ccaaagaaac ctgaaaagag ataggcgtga ttgagctggt cttgttctaa gatacggaca
     3541 aaacggtcaa actgagctgg ttgccaagcc tttagttgat cttgtttcat ttggccaagc
     3601 ccattccgtc aaacaagaca gccttggtag tttccacaac ttgctccaaa gggagactag
     3661 catcaatctt gacaatgcga tttccctctt tatccagaag agaaaggtag ccttgacgaa
     3721 cttttttatg caagtccaac ccttccaaat ccaaacgatt aacctcgcgg tcactattag
     3781 cagcaatacg agccagccct tcttccacct cgatgtcaaa atagagtgtc aaatcgggtt
     3841 tgaggccatc tgtcgcaaac tgattgagcc agtcaatggc ttcaatatct aagccacgac
     3901 caaatccctg ataggcaaca gaactatcga taaaacgatc catgatgacc aacttgccag
     3961 cttcaagggc tggaagaact ttttccacca aatgctgtct gcgactggca atatagagaa
     4021 gtagctctgt tttagcatcc atctgagtat gacttggatc caaaatcact tcccgaatct
     4081 tctccccaat caagactccg ccaggttcac gggtcgtcag cacctttact cctttttcct
     4141 ctaaaattgg tagcagagcc tctaaaacac tggtcttgcc tgctccctct ggtccctcaa
     4201 gagagactaa aaatcctttt gacatgtcta actcatttct tttttactac ttctattcta
     4261 tcaaaaaaat aggggtttgt gacaatcttt ttgtcttctc attt
//
LOCUS       DAVBGY010000093         4242 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVBGY010000093 DAVBGY010000000
VERSION     DAVBGY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.093"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(60..659)
                     /locus_tag="VQ620_001951"
     CDS             complement(60..659)
                     /locus_tag="VQ620_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2764526.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(660..1757)
                     /locus_tag="VQ620_001952"
     CDS             complement(660..1757)
                     /locus_tag="VQ620_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2764527.1"
                     /translation="MLERLKSIHYMFWASLIFMIFPILPVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLFWGIMLIYVAGNTAFVAVNYIWFFFFLSNLLSYHFSVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYLLGILTFVDLMTLGSVRIRLVEDLKEAQ
                     TKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQIQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVEIANQLDTASLTQEL
                     ESTASMILLELVTNIIKHAKASKAYLKLERTDQELLLMVRDDGKGFETVTENELHTVR
                     ERVLPFSGEVSVISQKHPTEVQVRLPYKERK"
     gene            complement(1757..2494)
                     /locus_tag="VQ620_001953"
     CDS             complement(1757..2494)
                     /locus_tag="VQ620_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2764528.1"
                     /translation="MKRWIALNKIEFLLTKRQSVYYLLSVGMPTAFYLFFSGMYQDTP
                     GGPANFMRDYLISMTAFSMMSTAIFSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMMVDYLVSILVVFSVGHLVRGVDMSLGSWIGAAFLLIVGSIAFVALGLTLTLLPSS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAIGKCLPTYQLMELLKTFLNEGSINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(2496..3380)
                     /locus_tag="VQ620_001954"
     CDS             complement(2496..3380)
                     /locus_tag="VQ620_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2764529.1"
                     /translation="MNMIKVQGLHKNIKSKAILKDISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVYIQDLPVTSSKLDYTRSYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLNNQEIDQLLQFDQQQKEQFAEKLSGGQKRLLSFILTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     EIEKHFILPLAYKEVVEKSVLVENWVQKQDALQVVTCEADAFWELLVQAGCRIQEIEV
                     NNRSLLDTIFEETQKGDD"
     gene            complement(3367..3561)
                     /locus_tag="VQ620_001955"
     CDS             complement(3367..3561)
                     /locus_tag="VQ620_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764530.1"
                     /translation="MKKAILFLFIGLSLVVWLVEMFTGWFDQSFLHQVIRGAWGLGFM
                     ISVVFPMGMEWLKGEYYEHD"
     gene            complement(3558..3764)
                     /locus_tag="VQ620_001956"
     CDS             complement(3558..3764)
                     /locus_tag="VQ620_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764531.1"
                     /translation="MEEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEGFYHFPWELFAS
                     FGIMSWLIREGLKLVRDVKKELEK"
BASE COUNT         1343 a          942 c          725 g         1232 t
ORIGIN      
        1 tattgttcca ttcactaccg tcaattttag cacatagtct tcatgaaaat attatatcat
       61 cataaccaac cagattcttt tgcgatatta gctgcctctg ttcgattacc agcatccagt
      121 tttgaaagaa tattggtgac atagtttcga atagttccgt ttgagaggta aagctgatct
      181 gcgatttctt ggttggataa gccccgggcg attcccttta agacagcaat ctcttgttct
      241 gtcaacggat tgggacgggt catcaccact tccatcaatt caggcgaata ctccttgcgt
      301 ccttcgagga cggtgtgcaa ggtttgcatg aggtctgcaa tgcttcgttc ctttaataca
      361 taagcatcca ctccagcctt gaccgcacgt tcaaaatacc cagcacgctt gaaggtcgtc
      421 accacaacca cctttgtttc aagcctttct gatcgtatcc actccaagac ttcaagacct
      481 gtcttaacag gcatttctac gtcaaggatg gcgatatcta cagactcctt ttctaatagt
      541 tggattgctt cttgcccatt cttggcttga aggacagact ccacatctgg ttgaagcgtg
      601 agcaattgac acatagcatc tcgcaacata ctttgatctt ctgcgactaa tactttcatc
      661 tactttctct ccttataagg tagtcgaact tgcacttccg ttggatgttt ctgactgatt
      721 acacttactt ctcctgaaaa tggaaggaca cgttctcgaa ctgtatggag ctcattttct
      781 gttacagttt caaaaccttt cccgtcatct ctgaccatca ggagcaattc ctgatctgtg
      841 cgttctaatt ttaagtaggc tttagacgct ttagcatgtt tgatgatgtt ggtcactaat
      901 tcaagcaaaa tcatggaagc cgttgactct aattcctgag ttaagctagc tgtatctagt
      961 tgattagcta tttccacctc aattccagca atttctaaca tctttttcac agtctctagt
     1021 tcggatgtca aagttctaga cttaagattt tccacaatgg ttcgcacttc acgcattgat
     1081 tctttgctaa tctgctgtat ttctcttaat tccttttcca cttgtggata agcctgtatt
     1141 tgaaataact gcaaggctag atcagtttta acactaagca tagcaaaggt atgccccaga
     1201 ctatcatgca aatcttgacc gatacgattg cgctcatttt ctgctagcaa tagatttatc
     1261 tgggtatttt gcttagtctg agcttctttc aaatcctcga caaggcgaat tcgaaccgag
     1321 cccaaagtca tcaaatcaac aaaagtaaga attccaagca gatagactag aaactcaact
     1381 tcgattctct gaaaaatcaa aagtcgcccc acaacaagga cttgagcaag aagaaaagtc
     1441 cagacatgta aagactttaa actacgtacg ctgaaatgat aacttaagag attggaaagg
     1501 aaaaagaaaa accagatata attcacagca acaaaggcgg tattcccagc tacatagatc
     1561 agcatgatcc cccagaaaag ccaagatagg cgctggctct tagttgttaa aacacccaaa
     1621 tatgccacta caaatagaat atcaatcaat aaatgccagg cagaaagcca cccagtcact
     1681 acaggtagga tggggaaaat cataaaaatt aaactggccc aaaacatata gtgtatgctt
     1741 ttcagtcttt caagcattaa gcattctcct tatgaccttg aaggtaaatg gtcaaaccaa
     1801 acaaaactgc tgaaaaaaca agtagataaa ctgtggctga tagattgatg ctaccctcgt
     1861 ttaagaaggt cttgagcaac tccatcaact gataagttgg taggcacttc ccgattgctt
     1921 gcatccagtc tggaaataaa gagatgggca tccagagtcc acctaaaaca gccaagccta
     1981 gatagagaag attgcccacg acagacatca gctgactaga aggcaagagt gtcaaggtca
     2041 agccaagcgc tacaaaagct atacttccta ctatcagcaa gaacgcagcc ccaatccagc
     2101 ttcctaaaga catatccaca cctctgacca agtgcccaac tgagaaaaca accaggattg
     2161 agaccaaata atcaaccatc atacttgtta tctttgatag ataatattct accatattta
     2221 cagggctatg acgcaatgtt ttctgccagt tgttcatctt atcggtatgt aagacagctg
     2281 ggaatgaaaa gatagcggtc gacatcatgg aaaaggcagt catggagatg aggtagtcgc
     2341 gcataaaatt agccggtcca cctggtgtgt cctggtacat gcctgaaaag aatagataga
     2401 aggctgtcgg catccctaca gataataaat aatagactga ttgccgtttg gtcaatagaa
     2461 attctatctt gtttagtgca atccatcgtt tcatcttagt catctccctt ctgtgtttct
     2521 tcaaagattg tatccaacaa actacgatta ttgacttcga tttcttgtat cctacatcct
     2581 gcttgaacta acagttccca gaaagcatcc gcttcgcatg tgactacttg taaggcatct
     2641 tgtttttgta cccagttttc aaccaagact gacttctcaa cgacttcctt gtaagctagc
     2701 ggaaggataa aatgcttttc aatctcctca ctacgcatag ctagaggcgt cgtgtcacga
     2761 atcaactctc ccttatttaa aaccaaaatc cggtcagctg tatgctctac ctcttcaata
     2821 taatgagacg aatagagaat ggtgactcct tgcgctttta ggtcccgaac aatttcccaa
     2881 aaacgttgac gagttgaagt atccatggca gcagttggct catctaaaaa gacaagcttt
     2941 ggtcgcccaa ttaaggtcaa gataaaagat aaaagacgct tttgcccacc tgacaatttt
     3001 tctgcgaatt gctctttctg ttgctggtca aactgtaata gttgatcgat ttcctggttg
     3061 ttcaagggat ttggatagat acgttgaaag aaagcaatca actctttaac ctttagtttt
     3121 tgaacaatta cattttcttg agggagatag gatctcgtat agtctaactt agagctcgtt
     3181 accggcaaat cttggatata tacttgccca ctggtgacca atttatctcc aagcaaacag
     3241 tccaagagtg tcgtcttacc agctccattt ggcccaatca aggcgacgca ttcaccttca
     3301 gctacctcaa aggaaatatc cttcaaaata gccttacttt tgatgttttt atgtaagcct
     3361 tgtaccttaa tcatgttcat aatattctcc tttcaaccac tccattccca tagggaaaac
     3421 gacggaaatc ataaatccta atccccaggc gccgcgaata acttggtgaa gaaaggattg
     3481 atcaaaccaa cctgtaaaca tttctaccaa ccataccact agtgatagtc caataaaaag
     3541 aaagagaatc gcttttttca tttttcaagc tcctttttca catctcttac taatttcaag
     3601 ccttctcgta tcaaccaaga catgattcca aagcttgcaa acaactccca aggaaaatga
     3661 tagaatcctt cgtccaatcc tgagaacatg agataagtca tgactcctgc tactactaaa
     3721 ctcactgcga taatcatttt atttctcatc tcttcttcct ccatttcata ctacaattat
     3781 agtcttttga aatcagagga gatagaagct tctgtcacta aaaaatatga caaatgtcat
     3841 aaaaaattct gttcaaaaca agcaagatac actatacaat aaaacacaat tagataaatc
     3901 taaggcaact tcctcaaaag agatatcaaa ccccattcac actataatgt aaactaatac
     3961 ttatttaaag tcaaaagagc ggaaattttt atcgtacaaa catatatatt ataggtaaga
     4021 caaaaaactc ttcaattact ttcttaaaaa tttataggag ttataaaatc tattaaattt
     4081 caatgtggat acctaaacta cagtatttat tgaaaagact ggagacaaaa agtatacgct
     4141 gccaaaatga attgctgaaa atcaaaaaag agagaagcaa actgattctc tcttaatgta
     4201 tataatatct agttataaaa atacacactc acatatctct gt
//
LOCUS       DAVBGY010000094         4193 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVBGY010000094 DAVBGY010000000
VERSION     DAVBGY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.094"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..139
                     /locus_tag="VQ620_001957"
     CDS             <1..139
                     /locus_tag="VQ620_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2764532.1"
                     /translation="LNYKSPREDFLMANLNLKFSVRNKSRNRFKFCRSFKYPARRCSW
                     C"
     gene            111..755
                     /locus_tag="VQ620_001958"
     CDS             111..755
                     /locus_tag="VQ620_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HET2764533.1"
                     /translation="MLRAGVLGANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGECKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSEYR
                     IPATVLIVGVALLLTGYTSARLGKDPTRTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(797..2785)
                     /locus_tag="VQ620_001959"
     CDS             complement(797..2785)
                     /locus_tag="VQ620_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2764534.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVLRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFVKNDGLKGQKTLTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(2787..3545)
                     /locus_tag="VQ620_001960"
     CDS             complement(2787..3545)
                     /locus_tag="VQ620_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2764535.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVMTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            3973..4164
                     /locus_tag="VQ620_001961"
     CDS             3973..4164
                     /locus_tag="VQ620_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764536.1"
                     /translation="MKHKEHILIGFLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
BASE COUNT         1266 a          827 c          829 g         1271 t
ORIGIN      
        1 cttgaactac aagtcaccca gagaagactt cttgatggct aacttgaact tgaaatttag
       61 cgtcagaaac aaatcacgaa atcgattcaa attttgcagg tcgtttaaat atcctgcgcg
      121 caggtgttct tggtgctaac gatggaatta tttccattgc tggtgtggtt atcggggttg
      181 ccagtgccac gaccaatatc tggattatct ttttatcagg atttgcggct atcttagctg
      241 gtgccttttc aatggctggt ggagaatatg tatccgtttc aactccaaaa gataccgagg
      301 aagctgccgt ttcgcgagaa aaactcttgt tagaccaaga tagggaacta gccaaaaaat
      361 ccctctatgc tgcttatatc caaaatggag aatgcaaaac ttctgcccaa ctcttgacca
      421 ataagatctt tcttaaaaat ccactcaagg ctctggtaga ggaaaaatat gggattgagt
      481 atgaagaatt taccaatcct tggcacgctg ccatttctag cttcgttgcc tttttcctta
      541 gaagtttgcc tccaatgctg tcagtgacca tattcccaag tgaatatcgc atccctgcta
      601 ctgtccttat tgtcggtgtg gcccttcttc tcactggtta cactagtgcc agacttggaa
      661 aggatccgac tagaacagct atgattcgga accttgctat tggtctcttg accatgggag
      721 ttaccttcct gctcgaacaa cttttcagca tttagaatac aagaaatacc tcgattttga
      781 agtcgaggta tcttttttac atttgcacaa tcttgcgata acttcttgaa gtaatcatga
      841 aaatcagcac ataggcgatg aggaagatag cgcagataga caaggtcaca atcaacatca
      901 tagtcgtatc cagtacacca atcactttta aaatcaggct aagcatatgg taggcaaagg
      961 cgagatgtat gaaggcaaag agcaaaggaa ggaagaaaac agttaaaacc tgtttgttga
     1021 tggtttgctt gatttgcttt tggtccaaac cgactttctg caagataata aagcgttcac
     1081 ggtcttcgta gccttcagaa atttgtttgt agtagatgac cagaacagtt ccgaccataa
     1141 agataatgga taggaaaata ccgataaaga agacaccacc aaagagggca ctcatctgag
     1201 aactagcatc tgctagattg ctaccataaa catagctacc ttctgtgtct aattgagcat
     1261 taaattgatt gaggtagttt tcatactcct cagcgacctt gagttgttct tcttcgctgg
     1321 catctacatt cataccaccg taaaactgat tatagatagc cgaatctggg aattgatcca
     1381 aaaaggcttg taaatcagga acaacaaggt aattgtaatc agtagtcaag atattaaact
     1441 tatttggaac atggttgaca atgaaatctt tattaaattc ttctttgaca gaaaattgat
     1501 gatcatttag agttagagtt ttctgtcctt tcagtccgtc atttttgaca aagagaccta
     1561 cctcatttcc tgatagagac agtttttgac cagtcatatt ttcataatct ttttggtcaa
     1621 ataccatgaa aactgttttt ggttggacac ggttttgtcc tttttcaaaa atagttaact
     1681 tggttccttc ttgattcgca acagcaaagt aagtgtaacg aagcacttct ttctctttaa
     1741 tcttataacc tttgtcactt gcaaactggc tcaagagttt gtccaaatct tctttttcaa
     1801 cattttgccc tgaaacccca aaatcatgag gatttagaac ttttttaaag gattctgcgg
     1861 aattgaaaat gcttgtcgct gctgacatgg ttaccaaaac cattgttgac aaaatagcga
     1921 tagttgctag tccaactgca tttttcttca tacggaaaat caagttagaa acagatatga
     1981 gattattagg ttggtaatag tatttcttat ttttctttaa gatttgaaga aaaacggtaa
     2041 tccctgcatt gaacaaaaga taagtaccaa agataaccag taaaacagct atgaagaagg
     2101 ttgttaaggc tgtaagagga tcttttaccg taagggcaag ataatatcca atccctaaac
     2161 ttatggaacc aagaatagtt tggagaggaa ggaagcgacc ttttttctct ccgctagctt
     2221 tctcacgtga gagctggagg gcattcatac gggcgattcg aagggcattc aggaacatga
     2281 ggcctaggaa aatcaaaccg aagacaagaa gtactgtaat gacaactttc atctggaagg
     2341 tagcgaccag ttccaccttc aatttcatca gtttgagtag gaaagcgaaa atcaacttgt
     2401 caaacaaggc tccaataccg attcctgctc caacagttag aatcccaaat agcactaact
     2461 ccttaaaggt catactgatt agatggcgct tctccaagcc taacatgcca tatataccca
     2521 gttccttgga acggtttttc atgacaaaac tattggcata gaggacgata atggctgacg
     2581 caagggtaac gacaaacata ccaaatccaa gtgttgcttg aatggtggtt cctccacgga
     2641 tttccgcaat cttgggattg aaggttaggg agtaaaagag ataggtgatg gtgactgcca
     2701 agagaacagc cagtgcaaag ggatagtaga gtttgcggtt tttaatcaag ttcgataccg
     2761 ctaacttatt ggttaatcga aacatactaa ttcacctcgc ttgccatgac agtcaaggta
     2821 tcagagattt cttggaacat ctgacgctct gtcttctctc cacggtagat ttggttgtaa
     2881 agaatgccgt ctttgataaa gagaacacgc ttggccctgc tagcagctgc tgttgagtgg
     2941 gttaccatga ggatggtttg cccacgctca ttgatttcat taaagacatc aagtaaggct
     3001 gcagatgact tggaatcaag ggctcctgtt ggctcgtccg caaggagaat ttcaggttct
     3061 gtgatgatgg cgcgggctac tgctacacgc tgtttctgac caccagaaat ctcgtaaggg
     3121 tacttctctt gcaattggtt gatacccaga ttttcagctg tcatcaccaa tttcttcatc
     3181 atctccgtta taggtcttct tgacaagaca agcggaagca agatattgtc cttaacagac
     3241 agagtatcta gcaagttaaa gtcttggaag acaaatccta gcttttcacg ccggaaacta
     3301 gaagcctgtg aatttttaat agttgctgta tcagttccgt ttaggtaaac ctgcccacga
     3361 cttggtttat ccagcatagc taggatattg agcagagtgg atttaccaga accagactca
     3421 cccatgatag caacgtagtc acccttttct acggtgaagt gaatatcctt gagggcctct
     3481 acttggttgc cctgaaaacg agttttataa attttttgaa cgtgttttac atctaaaagt
     3541 gtcatgagaa tctcctttct taatatccca tcaaatgaaa tgtagcttgc atcatagtgc
     3601 atcccagctg aatgcgctca atttctttgt gacttggttt tctagttttc ttatgtaaga
     3661 tttgtttcat gatgttactc ctttgttctt tatgagtcta gtttacatca aaaaaagacc
     3721 gcttgccata acctaacctt acaaaagaga ctttaatctt acatttttgt aagattgggt
     3781 gaaatataag caaaattact tagaaaatga tttgatttcc tatttttaaa gttgtaaaat
     3841 ataagtatga aaaggcttac tttaaaaagg aggaagcggg aactaacatt ttagtaaggt
     3901 tacctagata acataccatt caatttaaca cttgcaagtc gttcaaccta taaggaaaga
     3961 aggtgcgttt taatgaaaca caaagaacat atactcatcg gatttctcta tctgctctct
     4021 ccattcatcg gtcagctctt agttgaacac acacacttta tcggtacaga atttaccggc
     4081 actgcttatg ttatttgctg gttatcagtt gttatcagca tccaccattt ttccaaaaat
     4141 gtattgtctc agcagcaaaa atagattaaa aaactgcatt taaattactg ctt
//
LOCUS       DAVBGY010000095         4192 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVBGY010000095 DAVBGY010000000
VERSION     DAVBGY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.095"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            720..1712
                     /locus_tag="VQ620_001962"
     CDS             720..1712
                     /locus_tag="VQ620_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2764537.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYDGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1714..2532
                     /locus_tag="VQ620_001963"
     CDS             1714..2532
                     /locus_tag="VQ620_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET2764538.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2534..3319
                     /locus_tag="VQ620_001964"
     CDS             2534..3319
                     /locus_tag="VQ620_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2764539.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3417..3635
                     /locus_tag="VQ620_001965"
     CDS             3417..3635
                     /locus_tag="VQ620_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HET2764540.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3620..3817
                     /locus_tag="VQ620_001966"
     CDS             3620..3817
                     /locus_tag="VQ620_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2764541.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEASNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNPQNPCQILIKKLE"
BASE COUNT         1160 a          721 c          909 g         1402 t
ORIGIN      
        1 gctatatatt atggtatcga atcttcatca gaatgataaa attaatcaat tgatatctga
       61 ttacaaacag aatatgaaag ctttttatat cactattgaa aaatttatac gagatgatga
      121 aagccttaag tgttatttta taaaggttat ttcaagtcgt tccaaggtaa caagtctaga
      181 tcagattgaa gctgataaaa ctatacaaag aaaatattca agtgagctaa aaaaatttat
      241 tggattttat aatgagatta tttgtgagga aaatagtttc ctacatgtac gaaagaggtg
      301 gtcgagttgg tttaggtagt cgatgcgtga gttgataatt ctcagggtat ggacttcttt
      361 ttcatgaatg aggtaaaaga gcaggtattg tttagagaca atcattctga gcatattttc
      421 tggatagagg gagtatccga ttttatgatc aaagttaata ccgccctctg gtgagaagat
      481 gagtaggttg gtaatttaaa ctattaaaca gaatttttga ttaaaagtat tattttatga
      541 gagaaatcct aatttcacaa tccataggca aactcttgca tttcgttttt tattggacta
      601 taataggttg gtataaagcc ttttgtagta ataaaatgta gaaggtgtag aaagcaagga
      661 tttagaatat ttgtagttaa aaacacaatg ttgctattcc ttacgatagg gagatagata
      721 tggcaatgat agaagtggaa catcttcaga aaaattttgt gaagactgtt aaggaaccgg
      781 gcttgaaggg ggctttgcgc tcctttattc atcctgaaaa gcagaccttt gaagcggtca
      841 aggatttgac ctttgaggtt ccaaaagggc agattttagg atttatcggg gcaaatggtg
      901 ctgggaagtc gacaaccatt aaaatgctga caggaatttt gaaaccaaca tctggttttt
      961 gtcggattaa cggcaagatt ccccaggaca atcggcaaga ttatgtcaaa gatattggcg
     1021 tagtctttgg acaacgcacc cagctatggt gggatttggc tctgcaagag acctacactg
     1081 tcttaaaaga gatttatgat gtgccagact cgctctttca taagcgtatg gactttttga
     1141 atgaagtctt ggatttgaag gactttatca aggatcccgt gcggactctt tcactgggac
     1201 aacggatgcg ggcggatatt gcggcctcct tgctccacaa tcccaaggtt ctttttttag
     1261 atgagccgac cattggtttg gacgtttcgg ttaaggataa tattcgtcgg gcaattactc
     1321 agatcaatca agaggaagaa actaccattc ttttgaccac tcacgatttg agtgatattg
     1381 agcaactttg tgatcggatt ttcatgattg acaaggggca agagattttt gatggaacgg
     1441 tgagccaact caaggagacc tttggtaaga tgaagactct ctcttttgaa ctgctaccag
     1501 gtcaaagtca tctcgtctct cactatgacg gtctgtctga tatgaccatt gatagacaag
     1561 gaaacagcct caacattgaa tttgatagtt ctcgctacca gtcagctgac attatcaagc
     1621 aaaccctgtc tgattttgaa atccgcgatt tgaagatggt ggatacggat attgaggata
     1681 ttatccgtcg cttctaccga aaggagctct aggatgatca aattgtggag acgttataaa
     1741 ccctttatca atgcaggggt tcaggagttg attacttacc gagtcaactt tattctctat
     1801 cggattggcg atgtcatggg ggcttttgtg gccttttatc tctggaaggc tgtctttgat
     1861 tcttcgcaag agtctttgat tcagggcttc agtatggcgg atatcaccct ctacatcatc
     1921 atgagttttg tgaccaatct tctgactaga tccgattcgt cctttatgat tggggaggag
     1981 gtcaaggatg gctccattat catgcgtttg ttgcgaccag tgcattttgc ggcctcctat
     2041 cttttcaccg agcttggttc caagtggttg atttttatca gcgttggcct tccattttta
     2101 agtgtcattg tcttgatgaa aatcatatcg ggtcaaggta ttgtagaggt gctaggatta
     2161 actgtccttt atctttttag cttaacgctc gcctatctga ttaacttttt ctttaatatt
     2221 tgctttggat tttcagcctt tgtgtttaaa aatctttggg gttccaacct acttaagact
     2281 tccatagtgg cttttatgtc ggggagtttg attcccttgg cattctttcc aaaggttgtt
     2341 tcagatattc tttccttttt gcctttttca tccttgattt atactccagt tatgatcatt
     2401 gttggaaaat acgatgccag tcagattctt caggcactcc ttttgcagtt cttctggctc
     2461 ttagtgatgg tgggattgtc tcagttgatt tggaaacggg tccagtcctt tatcaccatt
     2521 caaggaggtt agtatgaaaa aatatcaacg aatgcatctg atttttatca gacaatacat
     2581 caaacaaatc atggaatata aggtggattt tgtggttggt gtcttgggag tctttctggc
     2641 tcaaggcttg aatctcttgt ttctcaatgt catctttcaa catattccat tcctagaagg
     2701 ctggaccttt caagagatag ctttcattta tggattttcc ttgattccca agggaatgga
     2761 ccatctcttt tttgacaatc tctgggcact agggcaacgc ctagtccgaa aaggggagtt
     2821 tgacaagtat ttgactcgtc ccatcaatcc tctctttcac atcctagttg aaacctttca
     2881 gattgatgcc ttgggtgaac tcttagtcgg tggtatttta ttgggaacaa cagtgaccag
     2941 cattgtttgg actcttccaa aattcctgct tttcctagtt tgtattcctt ttgcgacctt
     3001 gatttatact tctcttaaaa tcgcaacagc cagtatcgcc ttttggacta agcagtcagg
     3061 cgccatgatt tacatcttct atatgttcaa tgactttgct aagtatccga tttctattta
     3121 caattctctt cttcgttggt tgattagctt tatcgtgcct ttcgccttta cagcctacta
     3181 tccagctagc tatttcttac aggaaaagga tgtgttcttt aacgtaggag gtttgatgtt
     3241 gatttctctg gttttctttg ttatttccct taaactttgg gataagggct tagattccta
     3301 cgaaagtgcg ggttcgtaaa agctaaagta agactaaaat caagaaagga acttatgatg
     3361 tttgtaattg aagaagtcaa ggatgaaaat caaaaaaggc agttgtcgct gaggttttga
     3421 aggatttgcc agaatggttt ggaatcccag aaagcacaca agcctatata gaaggaacca
     3481 cgacactgca agtttggacc gcctatcagg agagtgattt gactggattt gtaaggttat
     3541 cctattcgag tgaagattgt gcagagattg attgtctcgg cgtaaaaaag cttatcaagg
     3601 tagaaaaatt gggagccaat tgcttgctac tttagagagt gaagctcgta aaaaagttgg
     3661 ttatctgcag gtcaaaacag tggcagaagc ttctaataaa gattatgatc gaacaaatga
     3721 cttttatcga ggtcttggct ttaaaaagtt agagattttt tctcagctat ggaatccgca
     3781 aaatccttgt cagattttga ttaaaaagct tgaataatat tacttgacat ctattctcag
     3841 agtgccatac tgtaagtgta atcgccgatt tagctcagtt ggtagagcaa ggcactcgta
     3901 aagcctaggt tataggtaga taaacgactg aggagttgaa aaaatagata ggtagaagat
     3961 aaccgttaag ccttattctt agcggttatt tatattgttt aatagcgctg atatttcatc
     4021 aattatgcct gttttcgtgt ttctggtagt tgttcaagtt tattgctact atttttgatg
     4081 gtatgaatgt gcttataatg tatcccggtt aacgaaagtt ttggacttat actcttcgaa
     4141 aatctcttca aaccacgtca acgtcgcctt gccgtgcgta tggttactga ct
//
LOCUS       DAVBGY010000096         4177 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVBGY010000096 DAVBGY010000000
VERSION     DAVBGY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.096"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(26..199)
                     /locus_tag="VQ620_001967"
     CDS             complement(26..199)
                     /locus_tag="VQ620_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HET2764542.1"
                     /translation="MNIKKRVLSAGLTFASVLLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYTDNIVN"
     gene            complement(357..1964)
                     /locus_tag="VQ620_001968"
     CDS             complement(357..1964)
                     /locus_tag="VQ620_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HET2764543.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2117..3601
                     /locus_tag="VQ620_001969"
     CDS             2117..3601
                     /locus_tag="VQ620_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HET2764544.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFLNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGISPADRKVAVVARQKAEETGGPALALELPNGDIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            3696..>4177
                     /locus_tag="VQ620_001970"
     CDS             3696..>4177
                     /locus_tag="VQ620_001970"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HET2764545.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
BASE COUNT         1206 a          957 c          799 g         1215 t
ORIGIN      
        1 gaggctcttt tgtcttgttc ttgtttcaat tgactatatt atcagtgtaa tagtctaata
       61 gatagttaaa tgtagttgga tttccactaa aggttgatga gtaagttttt gtatctgaac
      121 ctgattggcc gcaagcagct aaaagcaaaa cagatgcaaa agtcaggcct gcactaagga
      181 cacgcttctt tatgttcatc ttctttctcc ttaatagtgg gaatttgtaa agttaattga
      241 atttcgagaa tgaaagttta taaaccttgg ttataaaaaa caaaggattt ctgcttttat
      301 acagtcctcc cttgttttta tatgatttca attttaaatt tttctgcaaa aaatatttat
      361 agtaattcca cacagaaagc atcccatgga actaagattt gtttttcaaa gacttcttga
      421 gctagggtgt tttcaatcaa gacagatttg acttttcctt ctactgtcaa gtcttgctct
      481 tcattggaca agttagccac aactaggaag cgacggtcgc catccttacg tatataagca
      541 aagaccttat cagccgtatc aagcaattca aagtcagctc gaactagcca gctattctcc
      601 ttgcgaattt ggaccagttt ctgataggta tagaaaatag aatctggatt tgccagcgct
      661 tcttggacat tgatcatctc gtaatttgga ttaaccgcca accaaggttg acctgttgag
      721 aaaccagcgt ttttgctctc gtcccattgc ataggggtac gggcattgtc acgtccaata
      781 acacggatac tgtccatgat ttcttcaatc ggaacacctt tttcaagagc ctcacgcgca
      841 tagttgagag attcaatatc ttctacttga tccagtgttt caaacggata gttggtcatc
      901 ccaatctcct caccttggta gatataagga gttcctctca taagatgaag caagattgca
      961 aaggctttgg cagatttttc gcggtattct tggtcatttc cccagattga gacaatacga
     1021 gggaggtcat ggttgttcca gaagagggaa ttccagccgt cctcaactcc taactctgtc
     1081 tgccatttgt tgaagatttc ttttaactta gcgatattca gctctttttg atagtgccat
     1141 ttaggctgac cttcctgata ctgaagaccg atatgttcaa actggaagac catagacaat
     1201 tcttgcccct ttggatcaga gtagagcttg gcaatctctg gcgttgctcc ccaagtctcc
     1261 cctactgtca agagatcctt atctccaaag gtcgcctgat tcatttcctt gagataggga
     1321 tggagcatag gaccattatt gactaccttc tcgtcaggaa ttttgccaat catgtcaata
     1381 acatccatac ggaaaccacc aataccctta tcaatccaga agttcatcat ctcataaatt
     1441 ttctggcgaa gtttttcatt ttcccagttg agatccggct gtttcttgct gaaaaagtgg
     1501 agatagtatt gacctgactt ttcatcgtat tcccaagcag acccactaaa gatagaatct
     1561 aaatcgttag gctcatcccg ccagatatag tagtctcgct cagggctatt aggattttca
     1621 caggcctcta caaaccaggc atgctcatcc gaggtatgat tgaccaccaa gtccatgatg
     1681 atacggatat cacgcttctt agcttctgca atcagctgat ccatgtcctc catggttcca
     1741 aaaatagccg caatcgcttg ataatcagca atatcgtagc cattatcatc catagggctg
     1801 tcataaacgg gagaaagcca aattgctgtg attcctagct tggctagata gtccaactta
     1861 ctggtaattc ctggcaaatc gccaattcca tctccattac tatccataaa actctttgga
     1921 tagacttgat agactacggc attatgccac catttttctt gcatctgttt acctcattat
     1981 tttcataatc tatagtctat gatagcactt tttaaaattt tgtgcaagcg tttgcttaat
     2041 ctttttaatt tcttttttag ctccgtagtt tcctaacttc caatttttta ttataataga
     2101 gtccagaggt aaaaaaatga aaaaacaagc ttttagttct gaacagtatt tgaatttaca
     2161 acgcgaccat attttggagc gcattaacca atttgacggc aagctctact tagagtttgg
     2221 cggtaaaatg ttagaagatt tccacgctgc tcgtgtcctt cctggttatg aacctgacaa
     2281 caaaatcaag ctcttgcaag aattgaaaga gcaggttgag gttgtgattg ccattaatgc
     2341 tagcaacatt gaacattcca aagcacgtgg cgacttgggc atttcttatg accaagaagt
     2401 tcttcgtttg attgacaaat tcaatgaatt agggattttt gttggttccg ttgtcattac
     2461 acaatacgct ggccaacccg ctgcagatgc cttcctcaac caacttgaga aaaacggaat
     2521 tgattcttat cttcattatc caatcaaagg atatccgacg gatatggatc acatcatttc
     2581 cccagaaggc atgggcaaaa acgactacat caaaaccagt cgcaacttga ttgtcgtaac
     2641 cgctcctgga cccggttctg gaaaattggc aacgtgtatg tccaatatgt accacgacca
     2701 aatcaatggc atcaagtctg gctacgctaa atttgaaacc ttccctgttt ggaatcttcc
     2761 ccttcatcat ccagttaact tggcttatga agctgccaca gctgaccttg atgatgtcaa
     2821 catgattgac cccttccatc ttcaaaccta tggagaaacc actgtcaact acaaccgtga
     2881 tatcgaaatc ttcccagtgc tcaagcgcat gttggaacgt attctcggaa aatcaccata
     2941 cgcttcaccg acagatatgg gtgtcaacat ggttggtttc gctattacag atgacgaggc
     3001 tgctgtcgaa gcttctaaac aagaaatcat ccgccgttac tatcaaacag ttcttgactt
     3061 caaagctgaa aaagttggcg aagctgccgt caagaaaatt gagttgctca tgaacgacct
     3121 cggtatctca cctgcagacc gtaaggttgc tgtcgttgcg cgccaaaaag cagaagaaac
     3181 tggcggacca gccctagctc ttgaattacc aaatggtgac attgtcactg gtaaaaactc
     3241 agaactcttt ggccctacag ccgctgcctt gatcaacgcc atcaaaaaat cagctgacat
     3301 cgctaaagaa gtaaaactaa tcgagcctga agttgttaag ccaatccaag gacttaaaat
     3361 cgatcatctc ggtagccgca atccacgcct tcattcaaat gaaatcctga ttgcacttgc
     3421 tatcacagct acagaaaatc ctgatgctgc ccgcgctatg gaagaactcg gcaacctcaa
     3481 aggaagcgaa gcccactcaa ccatcatctt gactgatgaa gacaagaatg tccttcgtaa
     3541 actgggtatc aacgtaacct ttgacccata ctaccaatac gaccgcttat atcgtaagta
     3601 aagatttcaa cctctccagc ctcggagagg ttttctctct gtctcaggag caagctttat
     3661 gatataatga gagaagaaaa ctgaaaggat ttaccatgtc aaaagaagtt attgtcgaaa
     3721 gttttgaact tgaccacacc attgtcaaag caccctatgt tcgcttgatt ggggaagaaa
     3781 caggaccaaa aggagacatc atctccaatt atgatattcg cttggtgcaa ccaaacgaag
     3841 actctatccc tactgccggc cttcacacta tcgagcacct cttagccaaa ctcatccgta
     3901 cccgcattga cggcatgatt gactgttcac catttggttg ccgcacaggc ttccacatga
     3961 ttatgtgggg acgtcacacc agtgctaaaa tcgcagctgt tatcaaggat tcgctcaagg
     4021 aaatcgctga aactactact tgggaagatg tcccagggac aaccatcgaa tcttgcggaa
     4081 actacaagga ccacagcctc ttctctgcta aagaatgggc aaaactcatc ttggaacaag
     4141 ggatttcaga tgatgccttt gaacgtcatg tgattta
//
LOCUS       DAVBGY010000097         3999 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVBGY010000097 DAVBGY010000000
VERSION     DAVBGY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.097"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            206..2107
                     /locus_tag="VQ620_001971"
     CDS             206..2107
                     /locus_tag="VQ620_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2764546.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGSFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINCVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
     gene            2110..2973
                     /locus_tag="VQ620_001972"
     CDS             2110..2973
                     /locus_tag="VQ620_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET2764547.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            3053..>3999
                     /locus_tag="VQ620_001973"
     CDS             3053..>3999
                     /locus_tag="VQ620_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2764548.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQVIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFILVTFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTI"
BASE COUNT         1197 a          680 c          881 g         1241 t
ORIGIN      
        1 gcgtacgtaa ataatgaaaa gaattaaata ataggcttgt gcgataacta tttgaaacaa
       61 gattagctgg ttagaaaatc caacaagata gtgaaggaat ctaatataaa atatacataa
      121 tttagtttat tttatgttat aaaagttgta ctaaaagact tacgattatc ttgaatgcct
      181 tagggaaaca tgctatacta ctcttatgat tattttacaa gctaataaaa ttgaacgttc
      241 ttttgcagga gaggttcttt tcgataatat caacctgcag gttgatgaac gagatcggat
      301 tgctcttgtt gggaaaaatg gtgcaggtaa gtctactctt ttgaagattt tagttggaga
      361 agaggagcca actagcggag aaatcaataa gaaaaaagat atttctctgt cttacctagc
      421 ccaagatagc cgttttgagt ctgaaaatac catctacgat gaaatgcttc atgtctttaa
      481 tgatttgcgt cggacggaga gacaactgcg tcagatggag ctggagatgg gtgaaaagtc
      541 tggtgaggat ttggataaac tgatgtcaga ttatgaccgc ttatctgaga attttcgcca
      601 agcaggtggc tttacctatg aagctgatat tcgagcgatt ttgaatggat tcaagtttga
      661 cgagtctatg tggcagatga aaatagctga actttcaggt ggtcaaaata ctcgtttggc
      721 tctagctaaa atgctccttg aaaagcccaa tctcttggtc ttggacgagc caactaacca
      781 cttggatatt gaaaccatct cctggctaga gaattacttg gtaaactata gcggtgccct
      841 cattatcgtc agccacgacc gttatttctt ggacaaggtt gcgacaatta cgctagattt
      901 gaccaagcat tccttggatc gctatgtggg gaattactct cgttttgtcg agttgaagga
      961 gcaaaagcta gctactgagg caaaaaacta tgaaaagcaa cagaaggaaa tcgctgctct
     1021 ggaagacttt gtcaatcgca atctagttcg tgcttcaacg accaaacgtg ctcaatctcg
     1081 ccgtaaacaa ctagaaaaaa tggagcgttt ggacaagccg gaagctggca agaaagcagc
     1141 caacatgacc ttccagtctg aaaaaacgtc gggcaatgtt gttttgactg ttgaaaatgc
     1201 ggctgttggc tatgatgggg aagtcctgtc acaaccgatt aacctagacc ttcgtaagat
     1261 gaatgctgtt gctatcgttg gtcccaatgg tatcggaaaa tcaaccttta tcaaatcaat
     1321 cgtggatcaa attccgttta tcaagggaga aaagcgcttt ggcgctaatg ttgaggttgg
     1381 ttactatgac caaacccaaa gcaagctgac accaagtaat acggtgctgg atgaactctg
     1441 gaatgatttc aaactgacac cagaagttga aatccgcaac cgtcttggat ccttcctttt
     1501 ctcaggagat gatgttaaaa aatcagtcgg catgctatct ggtggcgaaa aagctcgttt
     1561 gcttttagct aaattgtcta tggaaaacaa taactttttg attctggatg agccgaccaa
     1621 ccacttggat attgatagta aggaagtgct agaaaatgcc ttgattgact ttgatggtac
     1681 cttgcttttt gtcagtcatg atcgttactt tatcaattgt gtggcaactc atgttttgga
     1741 attgtctgag aatggttcaa ctctctacct tggagattac gactactatg ttgagaagaa
     1801 agcaacagca gaaatgagtc agactgagga agcttcaact agcaatcaag caaaggaagc
     1861 aagtccagtc aatgactatc aggcccagaa agaaagtcaa aaagaagttc gcaaactcat
     1921 gcgacaaatc gaaagtctag aagctgaaat tgaagagcta gaaagtcaaa gccaagccat
     1981 ttctgaacaa atgttggaaa caaacgatgc cgacaaactc atggaattac aggctgagct
     2041 ggataaaatc agccatcgtc aggaagaagc tatgcttgag tgggaagaat tatcagatca
     2101 ggtgtaaaga tggaacatct tggaaaagta tttcgtgaat ttcgaacaag tggaaattat
     2161 tctttaaagg aagcagcagg cgaatcctgc tctacctctc agttatctcg ctttgagctt
     2221 ggggagtctg acctggcagt ctcccgtttc tttgagattt tggataacat tcatgtaaca
     2281 atcgaaaatt tcatggataa ggcaaggaat tttcataatc atgaacatgt gtctatgatg
     2341 gcacagatta tcccacttta ctattcaaac gatattgcag gttttcaaaa gcttcaaaga
     2401 gaacaacttg aaaagtctaa gagttcgacg acttcccttt attttgagct gaactggatt
     2461 ttgctacaag gtctgatttg tcaaagagat gcgagttatg atatgaagca ggatgatttg
     2521 gataaggtag cagattatct cttcaaaaca gaagaatgga ccatgtatga gttgattctt
     2581 ttcggtaacc tctatagttt ctacgatgta gactatgtca ctcggattgg tagagaagtt
     2641 atggagaggg aggaatttta ccaagagatt agtcgccata agagattagt gttgattttg
     2701 gccctcaatt gttaccagca ttgtttagag cattcttctt tttataatgc caactatttt
     2761 gaggcttata cagagaagat tattgacaaa ggtattaagc tttatgagcg taatgttttc
     2821 cattatttaa aaggttttgc cttatatcaa aaaggacagt gtaaagaagg ctgtaagcag
     2881 atgcaagagg ccatgcatat ttttgatgtg ttaggtcttc cagagcaagt agcctattat
     2941 caggaacact acgaaaaatt tgtcaaaagt taattttccc aaataaggga aaaaataaaa
     3001 agctcctttc ggttttgata caatagtttc aaaatttgag aggagcttta atatgaatcg
     3061 ctatgcagtg cagttgatta gccgtggggc tatcaataaa atgggaaata tgctctatga
     3121 ttatggaaat agtgtctggt tggcttctat ggggactata ggacagacag ttttaggaat
     3181 gtatcagatt tctgagctcg tcacatctat tctcgtcaat ccctttggcg gagttatttc
     3241 agaccgtttt tctcgtcgta agattttaat gacggcagat cttgtttgtg ggattctttg
     3301 tctggctatt tctttcataa ggaatgatag ctggatgatt ggcgctttga ttgttgctaa
     3361 cattgtgcag gttattgctt ttgccttttc tcgcacagcc aataaagcta tcataactga
     3421 agtggtggag aaagatgaga ttgtgatcta taattctcgc ttagagctgg ttttgcaggt
     3481 tgtaggtgtt agctctcctg ttctttcctt ccttgtttta cagtttgcaa gtctccatat
     3541 gacgctactg ctagactcgc tgactttttt cattgctttt attctagtga ctttccttcc
     3601 aaaagaggaa gcaaaagttc aagagaaaaa ggcttttact gggagagata tttttgtaga
     3661 tatcaaggat gggttacact atatctggca tcagcaagaa attttcttcc ttttgctggt
     3721 agcttccagc gttaatttct tttttgcagc ttttgaattt ctacttccct tttcgaatca
     3781 gctttacggg tcagaaggag cctatgcaag tattttaact atgggggcta ttggttccat
     3841 cattggggct cttctagcta gtaaaattaa agctaatatt tataatcttt tgattttact
     3901 ggctttgaca ggtgtcggag tttttatgat gggattacca cttccaactt ttctttcctt
     3961 ttctggaaat ttagtttgtg aattgtttat gacgatttt
//
LOCUS       DAVBGY010000098         3237 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVBGY010000098 DAVBGY010000000
VERSION     DAVBGY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.098"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(175..786)
                     /gene="rpsD"
                     /locus_tag="VQ620_001974"
     CDS             complement(175..786)
                     /gene="rpsD"
                     /locus_tag="VQ620_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET2764549.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(981..2033)
                     /locus_tag="VQ620_001975"
     CDS             complement(981..2033)
                     /locus_tag="VQ620_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET2764550.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2030..2728)
                     /locus_tag="VQ620_001976"
     CDS             complement(2030..2728)
                     /locus_tag="VQ620_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2764551.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2895..>3237)
                     /locus_tag="VQ620_001977"
     CDS             complement(2895..>3237)
                     /locus_tag="VQ620_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2764552.1"
                     /translation="TIKINVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPAD
                     TMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLG
                     GLGLKNKKEEN"
BASE COUNT          902 a          729 c          622 g          984 t
ORIGIN      
        1 aggattatac cacattgtgt actatttttg gttcatttta ctatatcact ttggactata
       61 tcggtctagt tatgtctcaa cgattatagc tggatactaa gagaaatcaa aaaagcacta
      121 agggagaacg tcccaaagtg cttaatatca aggcctatct tattcaataa aatattacaa
      181 cattttgttg tagaattcaa cgacaagtgc ttcgttgatt tctgggttga tttcgtcgcg
      241 ttctggcaag cgagtcaatg aaccttccaa cttttcagcg tcgaatgata cgaatgctgg
      301 acgtccaaga gtagcttcta ctgcttcaag gattgctgga actttcaatg atttttcacg
      361 aactgagatc acttgacctg gagttacgcg gtatgatggg atatcaacgc gtttcccgtc
      421 aacaaggatg tgaccgtggt ttacgaattg acgagcttga cgacgagtag tcgcgagacc
      481 aagacggtaa acaacgttat ccaaacgacg ttccaaaaga agcataaagt tgaaacctag
      541 gattccgcct ttgatttttg tagcttgtac gaacaagtta cggaattgtt tttcacctac
      601 accgtaagtg aaacgaagtt tttgtttttc agccaattgc aaaccgtatt ctgacaattt
      661 agaacggttg tttggtccgt gttgtcctgg tacgtagtta cgacgtgcca attctttacc
      721 tgtacctgta agtgaaaggc caaggcgacg agcttgtttc caagatggtc ctgtataacg
      781 tgacatatga atgtcctcct gatataaata atatttcggt ggaaatagtc acttagaaag
      841 ccctgattcg tgcagatgcc cttcgcctaa acagccaagg ttacttgtca taagacacct
      901 gttgacgagc ttcatgcttt cctgctgata tttcacacaa aatctattct accatgaata
      961 gctggatttg taaaggggtt ttaggcttta ttttcactac cagagaggtt gagaacgagg
     1021 gtaaaggtac ttcctagacc gtactggctg ctgactgtga tttccccacc caattgatgg
     1081 gccaattcac gcgcaatcgc aagtcctaat ccatgaccac ctgtcttcat gttacgcgaa
     1141 gtttcgacac gataaagacg tttgaaaata ttttccaaat cctctggggc aataccctgc
     1201 ccttcatcgg tcacactgat tgaaagctgg tccttctcca gcttagccac cacttccagc
     1261 ttggttcctg gagcagaata tttaaaagcg ttatcgacca gattcaccaa gatacgagaa
     1321 agcttagcat aatctccctc aatccgggca gactctggga ttacctgcaa gtggacatct
     1381 cttctctcct gctcaatcaa aaactgaaat tcactcatgc actcaattaa gagcttgtcc
     1441 agaaaaatac tgtctttact ggtagtttcc acctgatttc tagctgtgtt tagggtcaaa
     1501 aaattcaact cctcaaccag tttattgagc ctctccgtct ggcgtccaat ggttgctaga
     1561 taatgagctt gctccgactc cttgataatc ccatccaaaa tcccttctac cgtcgcttga
     1621 atcgaagtga taggggtctt aatatcatgc gacaactggg caatcatcaa gcccttttct
     1681 cgttcgcttt cttccaagga atcaaagctt acctgcaaat catgggacat ctcattaaaa
     1741 gtttgcccta attgctgaaa ttctacagga ccttgaacct ccaaatttga aggaaaatcc
     1801 ttggccgcta cccgcttggc atgctccttg agtttgccca acgacgtaaa gactggcaat
     1861 aggagaaaga gactaatccc agcaccgaca aggctggcaa cgatggtcat cccaagcaaa
     1921 aagtaaatct cgcctttcgc aatcagcatt ttttgaacag cccaaaaaac aaccaaaatg
     1981 gttaagaggg ttgaaataat atatccaacc aaaatataac tttttagttt catgtttgtc
     2041 ctctcggttt ctctatctta tatcccaacc cccaaactgt cttaatagtg ggcgtttggt
     2101 cactactata ttttgccagc tcctgtcgaa gagcatggat atgcacattc aaggtattgg
     2161 tgtcatccac gtagtcttct ttccagatct tttcatagag gtctgtcttg gagaaaactc
     2221 gctctggatt gctagctaaa atccacagca attcaaatga tttaacagtt aaatccagca
     2281 tttcttctcc tatttgaact tcatgactac tatgattcat ttttagattg ccaagggaaa
     2341 tcagctctgt ttcgccccca cgatgaaggc ggcgcaaaat attgtggaca cgcaaaacta
     2401 gctcacgtgg gctaaaaggc ttagcaataa aatcatctgc tcccaagctc aggccgtaaa
     2461 tcttgtcctg ttcactggtc ttagcagtaa taaataggaa aggctgctct ggtgataagt
     2521 attgaacctc actgattaaa tcataaccat ccatccgagg catcatgaca tctgtgataa
     2581 tcaaatcaat cggttttttc ttgaacagct ctagcgcttc cagtccatca tgggctacca
     2641 agacctgata acctgcctga attaagtatc tctgatgaat atctgtgatt tctacctcgt
     2701 cgtcaacgag taaaattgtc tttcccatct gtctctcctt tgataaaaac agtgctatac
     2761 tgctttaagt ataacactat ttttattgtt ttacgatgat tttaatctgc tattgaaaga
     2821 aagtcctgat tgctgattga gttgttaaac taatcttagt cagcccacta tccatcattt
     2881 atttccttag ctgattagtt ttcttctttt ttgtttttca aacctagacc accgagtaaa
     2941 cctgcaagcg caagcccaag gaaaccaata cttgccattg atgtttgagt ctcaccagta
     3001 tttggtagca tagctttatc ctctgacatc atcgtatcag acatcttgtt agcagaagca
     3061 gccatgtttt cacctgccat cgtgttggta gaacttgtca tggtgtcagc aggcatgcta
     3121 tctgtagtac ctgtagcatg attgtgattc atcggagtca cgccagaacc agagttagaa
     3181 ggtgataatg aaccatttgc tgtgtctgat gtttctttaa catttatctt aatagtg
//
LOCUS       DAVBGY010000099         3192 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVBGY010000099 DAVBGY010000000
VERSION     DAVBGY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.099"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(381..914)
                     /locus_tag="VQ620_001978"
     CDS             complement(381..914)
                     /locus_tag="VQ620_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HET2764553.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(1003..1779)
                     /gene="recX"
                     /locus_tag="VQ620_001979"
     CDS             complement(1003..1779)
                     /gene="recX"
                     /locus_tag="VQ620_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HET2764554.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDEETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1817..3172
                     /gene="rlmD"
                     /locus_tag="VQ620_001980"
     CDS             1817..3172
                     /gene="rlmD"
                     /locus_tag="VQ620_001980"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET2764555.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT         1028 a          563 c          580 g         1021 t
ORIGIN      
        1 atttcatttt actatactgt tacgaaatta gatatataaa tgaaaacttg cctcaaggga
       61 gtttttttgc tagtagaaaa gcgatagaca gaaattgttg gactcttaaa aaaatgaagt
      121 tttgctcaaa agagcttctt aaattaaaac gagcaattat cagttcaaat ctgtttcaaa
      181 agaagaacgg ataaattaat cgatgctttg tttttgagtt aggaagttaa gcatgccaaa
      241 ctgagtcgta ggaataaatt tctttataga aatgtgttgt aactctgcta taatggattt
      301 attccttttt gtgtttacac aatttatttt atactaccaa aaaaacaagg tcaggatttt
      361 gttcctgacc tttgacaact ttaccgattc tttagttcta catagcgctt gtaccaaatg
      421 tttacatagg cttctgagaa aggaccacgt ccattgttaa tccaatcaac aagaattttg
      481 acatgttctt ttaaaatata gtccaagtca tcagaataat tcattttgcg tttgtgacgc
      541 tcgtactctt caacgtccaa gagacgtttt tccccatctg taaaaatttt aacatccaaa
      601 tcgtaatcaa tatacttcag tgcttcttca tccagatagt aggggctagc catattgcaa
      661 tagtaagaag ttccattatc acgaatcatg gcaatgatat taaaccaata tttcttgtga
      721 aagtaaacaa tagccggttc tcgagtgacc caacgacgac catcactttc ggtaacaagt
      781 gtatgatcgt tgacaccaat aatggcgttt tctgttgttt ttagtaccat ggtgtcccgc
      841 caagttcggt ggagactccc atcatgctta taactttgaa ttgtaataaa gtcgccttct
      901 tttggaagct tcataactaa ccaactttct acaatttata agtttatcat ttactattgt
      961 accataaaat tacctaaaat ctgtgaattt cacttggaaa tattaaagat attctctaag
     1021 agcgcttgct atatccgaaa aatcgtagcc ctttcgtgct aaaacttgag ttaaacgctg
     1081 cttcagttcg tatccttcat actttcgggc atacttagta tattgcttat caagttcctt
     1141 gaagatgagt tcctgagtcg tttcttcatc aacttgacta tccaattcgt caaaggcaat
     1201 tttagcatca aaataagaga agcccttgtt agtcaagttc tggataatct tatcttgcag
     1261 ggcacgagct ggaagttttc cctcatattt tttcaatagt ttattggcta cacgttgagc
     1321 aacttccgaa aaatcaaaat cattcaagat ttcttctata gtagattttg aaattccttt
     1381 ttgtgctaat ttctgagtca gtacataagg tcccttgtct cctgaaagtt gattggcatt
     1441 gatgatagca taagcgtact gaccatcatt aatccactta tcttctttaa gattagcaat
     1501 aacttgagaa acgatgtttt tatcaatatc gtattttttc agatattctc tgacttcttt
     1561 ttcagtgcgt gctttaaagg ataagtggta gagggccaga ttcttaccat aagaaaattg
     1621 agcaaagtct tgaatctctt tcaattcctc ttcgcttatc accttatctc tcgataacat
     1681 aaaacgaaca attgtatctt cggtgatata gcatttgtcg ccattatcaa gctccatcag
     1741 atagagtctt tttttctttt caagttttgt gattttcata gctctattat aactcaaaat
     1801 gtgataagat aggggtatga atctgaaagt gaaacaaaaa ataccattaa aaatcaagcg
     1861 catgggaatt aacggtgagg gaatcggctt ttaccaaaaa acattagtct ttgtaccagg
     1921 agctctcaaa ggcgaagata tctattgtca gattacttct attagacgca actttgttga
     1981 agcaaaatta ctgaaggtca acaagaagtc taaatttcga attgtgccat cttgtactat
     2041 ttataatgaa tgcggaggct gccaaatcat gcacctgcat tatgataagc agctggagtt
     2101 caagacggac ttacttcatc aagcgctgaa aaaatttgct cctgcaggat atgaaaatta
     2161 tgaaattcgt ccaactattg gaatgcagga accaaaatat tacagagcta agttacaatt
     2221 tcagactcga aaatttaaaa atcaggtcaa ggcgggctta tatgcacaaa actctcacta
     2281 tttagtagag ttgaaagact gcctggtaca agataaggaa acccaagtga ttgctaatcg
     2341 cttagcagaa ttacttactt atcaccagat tccaatcacg gatgagagaa aagttctagg
     2401 tgtccgtact attatggtcc gacgcgcgag aaagaccgga caggttcaga ttattattgt
     2461 tacaaaccgc cagcttaatt taactcaatt ggtaaaagag ttggttaaag atttcccaga
     2521 agttgtgaca gtagctgtta atacaaatac agctaaaacc agtgagatat atggtgaaaa
     2581 gacagagatt atctgggggc aagagagtat tcaagaaggt gtactcaatt atgaattttc
     2641 actatcccct cgagcttttt atcaactaaa tcctgagcaa acagaagtcc tctatagcga
     2701 agcagtaaaa gcgctggatg ttgataaaga agaccatttg attgacgctt attgtggagt
     2761 tggaacgatt ggatttgcct ttgcaaagaa agtaaaaaca ctcagaggta tggatattat
     2821 tccagaagct attgaagatg ccaagcgaaa tgctaaaaga atgggatttg acaatactca
     2881 ttatgaagct ggaacggcag aagagattat tcctcgttgg tacaaggaag gctaccgagc
     2941 agatgctttg attgttgacc caccacgtac aggtctggat gataagttat tagatactat
     3001 tcttacttat gtaccagaaa aaatggttta tatttcttgt aatgtttcga ccttggctcg
     3061 tgatttggta cgcttagtag aagtctatga tcttcattat atccagtcgg tcgatatgtt
     3121 cccacataca gctcgaactg aagctgttgt aaaattaata aaaaaagttt aaaaaaggtg
     3181 ttgacaaaac at
//
LOCUS       DAVBGY010000100         3160 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVBGY010000100 DAVBGY010000000
VERSION     DAVBGY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.100"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..314
                     /locus_tag="VQ620_001981"
     CDS             <1..314
                     /locus_tag="VQ620_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764556.1"
                     /translation="KKGQFDELVSNLNLSEQEQLDLLQQYKEEHPRRAKRGIKSAIIK
                     KVARFLAAKVGQKSVVEITDYLFEWQDNLEAGAENYLVQYGWDRNIAHWTIKTVSFIF
                     L"
     gene            801..1763
                     /locus_tag="VQ620_001982"
     CDS             801..1763
                     /locus_tag="VQ620_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HET2764557.1"
                     /translation="MEKWENQDKILLDKNKRGKDRNWRGRKLLSLKLADIFKELGYRE
                     TLIERVETCGDTLRFIRREDGSLRLYQAYFCKNKLCPMCNWRRSMKYSYQTSQIVDEA
                     IKEQPKGRFLFLTLTVKNVPGKELNATISQLTQSFDRLFRRAKVKKNLIGFLRSVEVT
                     HNQEEETYHPHIHVLMMVKSSYFSGAGDNYVSQEEWGRMWEQSLKVDYVPMVDIRSVK
                     EIGKGLKGAILETAKYPIKPIKLDVENKQVVGDLYNGLYRKRQLGYGGLFKEIRKRLQ
                     LSNVENGDLVYTSDDNDEMLKGTKIVAIWNATKQNYFVKNKGWN"
     gene            2236..2535
                     /locus_tag="VQ620_001983"
     CDS             2236..2535
                     /locus_tag="VQ620_001983"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764558.1"
                     /translation="MMNIAYKNRDLSDEEFKEEAHQFRDKEIKILEEKLKELNISCKV
                     VGINSIKDMNEYKEIMENVNQAKEELKRIDELIAVRSSRIDFLEDKTLRITGNQR"
     gene            2998..>3160
                     /locus_tag="VQ620_001984"
     CDS             2998..>3160
                     /locus_tag="VQ620_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764559.1"
                     /translation="MNKKFLKCGTLFLISCSILGSTIPAVTVFSDEVTITYNSENNSE
                     KNELYNQLSA"
BASE COUNT         1117 a          364 c          654 g         1025 t
ORIGIN      
        1 aaaaaaaagg acagtttgat gagttggtaa gtaatttgaa tttgagcgaa caagaacaat
       61 tagacttgtt gcagcagtat aaagaggaac atcctcggcg agctaaaagg gggataaaat
      121 cagctataat aaaaaaagtc gctcgtttcc ttgctgctaa agtaggacaa aaaagtgtag
      181 tggagataac tgactattta tttgaatggc aagacaattt agaagctgga gcggagaatt
      241 atcttgtaca atatggttgg gatagaaata ttgctcactg gacaataaag acagttagct
      301 ttattttctt ataagtcatt atgaataaag cttggttatt ttggagtgtc atagtcttat
      361 atttttttat aaagtttttt gataaagttt tagatataaa gctttttggt tctattcaaa
      421 tatggctaga taatctccct attccgatga aaatactgtt aacaatagca ttagttttgt
      481 ttttattcat tgtatttcca tatagaggaa aacggtaaaa acggtaatta gatatataaa
      541 aggagctgaa agctccgtaa aaaacctact cgaagagtag gttttttctt atcttgatac
      601 tagtaggaaa taactctcat gctattttta gggtttcgac agggtctaaa cccttgatac
      661 tactgacttt ttaggtaaaa aaatgccctt gacttttttc aacggatctt ttataattta
      721 gttgttcaga aaaaattata aaaagagaaa aaaggcaaag gtatttttgt atttctttgt
      781 aattacaggt aaattataac atggagaagt gggaaaatca agataaaatt cttttagata
      841 aaaataaaag aggcaaggat agaaactggc gaggacgaaa attattaagt ttgaaattgg
      901 cagatatttt taaagagtta ggttataggg aaaccttgat agaacgcgtt gagacttgtg
      961 gagatactct tcgatttatt cgtcgagaag atgggagttt gcgtttatat caagcctatt
     1021 tttgtaagaa taaattgtgt cctatgtgta attggagacg gtcaatgaaa tattcttatc
     1081 aaacatctca gatagttgat gaagcaatta aagaacaacc aaaagggcgt tttcttttct
     1141 tgactttgac tgtgaaaaat gttccaggta aagaattgaa cgcgacaatt agccagctaa
     1201 ctcagtcctt tgaccgtctt tttagacggg caaaggtaaa gaaaaatcta atcgggtttt
     1261 tacgttcggt tgaggtgact cataatcaag aagaagagac ctatcaccct catatccatg
     1321 ttttgatgat ggttaagtct agctatttta gtggggctgg agataattat gtatctcaag
     1381 aagaatgggg gcggatgtgg gaacagtcgc ttaaggttga ctatgtgcca atggttgata
     1441 ttcggtcagt taaagaaata ggaaaaggtc ttaaaggggc aattcttgaa acggcaaaat
     1501 atccaattaa gccgattaag ttagatgttg aaaacaagca agttgttggt gatttgtata
     1561 atgggttata tcgtaaacgg caattgggtt acggtggatt gtttaaagaa attcggaaga
     1621 gattgcaact gtctaatgtc gaaaatggag atctggttta tacttctgat gacaatgatg
     1681 aaatgttaaa aggtacaaaa atagtcgcga tttggaatgc gactaaacaa aactattttg
     1741 taaaaaataa gggttggaat taaagtatca actcttttat tataacataa aaaagagtaa
     1801 aatagacttg atttttttga gtttagatgt atattgaagg tgtgaaaatt aaaaagatag
     1861 ctatgttttt tctgggtata cttgttgggg tatttattgt tctatttttt agaaattatt
     1921 ttgttttact tttggaatat atttttggtt aaaaagcacc ctctaaaaag ggtgcttttt
     1981 gtgcggttat tcgcacgaaa gttctctgca agagttcctc ggaagagcgt ttgtattttg
     2041 attgtacgtc aaaactataa aaacatttac tctttgtcaa agagtagcag aaaagctaga
     2101 ataaaattaa aaattttatt ctagctaata gtgcaagtaa tagcttttgt agcttgcctc
     2161 ttgtctgaat aacggacaag aggcaggagt ttgtatttgt tctaaatgag tatttcattt
     2221 agaacaaata catcgatgat gaacattgct tacaagaata gagatttgag tgatgaagaa
     2281 tttaaagaag aagctcatca gtttagagat aaagagataa aaatactcga agaaaaatta
     2341 aaggaactta atatttcttg taaggttgtt ggaataaata gtataaaaga tatgaacgag
     2401 tataaagaga ttatggaaaa tgttaatcaa gcaaaagagg aattaaagag aatagatgaa
     2461 ttaatagccg tcaggagctc cagaattgat tttttagagg ataaaaccct tagaattaca
     2521 ggaaaccaaa gatgagctta aaaacgaaat ggagagccta aaaagagatt tatctttttt
     2581 tagaaacgaa aatactcgac tgaaaaatgt agtttcagat tgtatcaagg tagtattttt
     2641 ggggtatttt tagtagaaag tatattgtaa tttctgagcg atttatgccg tgaaagctac
     2701 ttgaccgtaa gctagatcaa agtacgctag tcagggacga gccgaaaggc tgtagcgttt
     2761 cggagcggca cggacaaagt acgtcgacct aggttacttg cggtcaagta gaccatggaa
     2821 taaaacaagc gggtatgata taaaaaagtt atcggcaaac cgaaatatag acttgttttt
     2881 ttgtttataa aaatgtatta ttatattgag aataagattg tttaatttat ttagagagga
     2941 gtgtaagttg atcagtaaac gattttaatt ctgcctttaa ataacaagga gtttgttatg
     3001 aataaaaaat ttttgaaatg tggtacttta tttttgattt cttgttctat tttaggaagt
     3061 actattcctg cagttacagt attttctgat gaagtaacaa ttacttataa ttcagagaat
     3121 aattcagaaa aaaatgaatt atataatcaa ctttctgctg
//
LOCUS       DAVBGY010000101         3102 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVBGY010000101 DAVBGY010000000
VERSION     DAVBGY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.101"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(119..751)
                     /locus_tag="VQ620_001985"
     CDS             complement(119..751)
                     /locus_tag="VQ620_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2764560.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(773..1645)
                     /gene="sdaAA"
                     /locus_tag="VQ620_001986"
     CDS             complement(773..1645)
                     /gene="sdaAA"
                     /locus_tag="VQ620_001986"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HET2764561.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(1654..2325)
                     /gene="sdaAB"
                     /locus_tag="VQ620_001987"
     CDS             complement(1654..2325)
                     /gene="sdaAB"
                     /locus_tag="VQ620_001987"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HET2764562.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            2567..>3102
                     /locus_tag="VQ620_001988"
     CDS             2567..>3102
                     /locus_tag="VQ620_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET2764563.1"
                     /translation="MKSITKKIKATLAGVAALFAVFSPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTA"
BASE COUNT          912 a          630 c          678 g          882 t
ORIGIN      
        1 atctacaacc ttaaaacagt gttttgagca gcccgcagct agtttcctag tttgctcttt
       61 gattttcatt gagtattact tcattttctt ctgaaataga gttgtcgccc agtctatgct
      121 attgaaaata cgccaaaact tctaagggtt tgtgagcgat ataatcaggt tgatagttta
      181 gtagatctgc ttgctctcca aatccccaag tgacggccaa tttctgaata cctgtttctc
      241 gagctcccag catatcaaac ttggtatctc cgatgatgat ggcttgttct ggtgctagtt
      301 gatgtgtctg taaggcttgg tgaatgacat ctgccttatg gggtgcttca gggctagaac
      361 cataaatacc atcaaagaaa tgatggattt ccaagttttt ggccatgtct tgagcagtag
      421 atgtattctt tgtcgtggtg atgtagagtg gataactgct cgataactcc tcaagcaagt
      481 ctataatctg aggaaagagt tgagcttcat agatgccttt tgccttatag taagaacgat
      541 atatctgcat ggcttcagaa atttggtctt tggacaggca ggtcgcaaaa ctactttcga
      601 gaggtggtcc cataaaacca cgaatagttt tggcatcagg gctaggcacc cccagctctt
      661 taaaggtata ggtaaaggca ttgtgaatcc cgatagaact atcaacgagg gttccatcca
      721 aatcgaaaaa aatcgctgtg atagaggtca tggtttctcc tatttgataa gcttattctc
      781 cgaaaatctc tttttggagg cgacgaccag taggggtggt agcgagtcca ccttcagctg
      841 tttcacgaaa ggcagttggc atgcttgctc ctacttggta catggcatcg atcacttcat
      901 ccacagggat tttagattcg atacctgcca aggccatgtc tgctgcgatg aaagcaaagc
      961 tagctcccat ggcattacgt ttgacacagg gaacttcgac caaacctgca acagggtcac
     1021 agatgaggcc tagcatattt ttaatgacaa aggcaatagc ttgactggcc tgataaggtg
     1081 ttccacctgc agccagagtc aaggcggcag cactcatagc agaggctgaa ccaacttcag
     1141 cttgacaccc accctcagca cctgagatgg aggcattgtt tgcgatgact agtccaaagg
     1201 caccagcagc aaagaggaaa tccaattgtt gctcgtggct gaggtctaat ttttcaatag
     1261 cagcagtgag aacggaaggg agacaaccgg cacttcctgc ggtaggagta gcgcagacca
     1321 agcccatttt agcattgtgt tcattgactg cgatagcatt tcgggcagca gagagaatcg
     1381 tgtaatctga taaagttttt tcgtttttga tgtagtgatc caatttggca gcatctccac
     1441 ctgtcaggcc actacgagat ttattttcat tgaggccaag ttggacagag gctttcataa
     1501 cttccagatt gcgttccatg agaaggaaga cttcttcacg ttcgcgaccg gtcaattcaa
     1561 actctgttgt aatcatgagt tctgcgacat ttccttgaaa gtccagatct gcttgctcga
     1621 ccaattcttt gatagaataa aacatgcttc ctcctattta aagaaattga cattgtggag
     1681 atgagggatt tttcgaattt cttcgatagc ctcatcacag ttgcgactgt caacttcgat
     1741 aatcataatg gctttttcac cagctttttc acgagtgaca ttcatctggg cgatattgat
     1801 accatagcgg gaaagcgcct ctgtaacaag ggcaatcata cctggaatat cttgatgaac
     1861 gatgatgata gtcggtgtat tcatattgag agagacggca aaaccattga gttcggttac
     1921 ctgaatattt cctccaccga tagaaatacc agtcacgctg atggtcttgt gggcattttt
     1981 aacagtaatt ttagtggtgt tagggtgagg ggcattgctg tctttctgaa tggtccagac
     2041 aatcttgata ccacgcttgt gggcaatttc cagactattt ggaatttcag gatcatctgt
     2101 atccattcct aaaatacctg caacaagggc taggtctgtt ccgtgaccac gataggtctt
     2161 ggcaaatgag ttaaaaagtt ggaattcaac ttctgtcgga gtatcatcaa aaatggaaga
     2221 gacaatcttc ccaatacgaa cagcaccagc ggtatggcta ctagatgggc caatcataac
     2281 tggtccgatg atatcaaaga cagattgaaa acgaagtgat ttcatcagtt tccccttata
     2341 aaaattctta tctctattat atcaaagaat gaggggcttg gctttaattg tggatgaaaa
     2401 cctttctaat acctcaaata gcataaaaat agtatctttt atgacaaaaa acaccttatt
     2461 tagggaaata aaaaataatt ttgtaatatt tctacataaa agtgtcaaga aacagtaata
     2521 tttaaagggt atgatagaac tatagaaaga aggagaattt tcgaatatga aatcaataac
     2581 taaaaagatt aaagcaactc ttgcaggagt agctgccttg tttgcagtat tttctccatc
     2641 atttgtatct gctcaagaat catcaactta cactgttaaa gaaggtgata cactttcaga
     2701 aatcgctgaa actcacaaca caacagttga aaaattggca gaaaacaacc acattgataa
     2761 cattcatttg atttatgttg atcaagagtt ggttatcgat ggccctgtag cgcctgttgc
     2821 aacaccagcg ccagctactt atgcggcacc agccgctcaa gatgaaactg tttcagctcc
     2881 agtagtaagt gaaacagttg tttcaactgt aagcggatct gaagcagaag ccaaagaatg
     2941 gatcgctcaa aaagaatcag gtggtagcta tacagctaca aatggacgtt atatcggacg
     3001 ttaccaatta acagattcat acctgaacgg tgactactca gctgaaaacc aagaacgtgt
     3061 agcagatgcc tacgttgcag gacgttacgg ttcatggact gc
//
LOCUS       DAVBGY010000102         2904 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVBGY010000102 DAVBGY010000000
VERSION     DAVBGY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.102"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            83..>2904
                     /locus_tag="VQ620_001989"
     rRNA            83..>2904
                     /locus_tag="VQ620_001989"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          834 a          584 c          854 g          632 t
ORIGIN      
        1 tctatatcaa atagtaacaa gaaaataaac cgaaaacgct gtagtattaa taagagttta
       61 tgactgaaag gtcagaaaat aaggttaagt taataagggc gcacggtgga tgccttggca
      121 ctaggagccg acgaaggacg tgacaaacga cgatatgcct tgggtagctg taagtaagcg
      181 atgatccagg gatttccgaa tgggggaacc caacaggtag tacctgttac ccacatctgt
      241 taaggatgtg aggaggaaga cgcagtgaac tgaaacatct aagtagctgc aggaagagaa
      301 agcaaaagcg attgccttag tagcggcgag cgaaacggca ggagggcaaa ccgaagagtt
      361 tactcttcgg ggttgtagga ctgcaatgtg gactcaaaga ttatagaaga atgatttggg
      421 aagatcagcc aaagagagta atagcctcgt atttaaaata gtctttgtac ctagcagtat
      481 cctgagtacg gcgggacacg tgaaatcccg tcggaatctg ggaggaccat ctcccaaccc
      541 taaatactcc ctagtgaccg atagtgaacc agtaccgtga gggaaaggtg aaaagcaccc
      601 cgggagggga gtgaaataga acctgaaacc gtgtgcctac aacaagttcg agcccgttaa
      661 tgggtgagag cgtgcctttt gtagaatgaa ccggcgagtt acgttatgat gcgaggttaa
      721 gttgaagaga cggagccgta gggaaaccga gtctgaatag ggcgccttag tatcatgacg
      781 tagacccgaa accatgtgac ctacccatga gcaggttgaa ggtgcggtaa gacgcactgg
      841 aggaccgaac cagggcacgt tgaaaagtgc ttggatgact tgtgggtagc ggagaaattc
      901 caaacgaact tggagatagc tggttctctc cgaaatagct ttagggctag cgtcgacatt
      961 agagattctt ggaggtagag cactgtttgg gtgaggggtc catcccggat taccaatctc
     1021 agataaactc cgaatgccaa tgaattatgg tcggcagtca gactgcgagt gctaagatcc
     1081 gtagtcgaaa gggaaacagc ccagaccacc agctaaggtc ccaaaataat tgttaagtgg
     1141 aaaaggatgt ggggttgcac agacaactag gatgttagct tagaagcagc tattcattca
     1201 aagagtgcgt aatagctcac tagtcgagtg accctgcgcc gaaaatgtac cggggctaaa
     1261 acaatttacc gaagctgtgg atacctttat aggtatggta ggagagcgtt ctatgtgtga
     1321 tgaaggtata ccgtgaggag tgctggaacg catagaagtg agaatgccgg tatgagtagc
     1381 gaaagacagg tgagaatcct gtccaccgta agactaaggt ttccagggga aggctcgtcc
     1441 gccctgggtt agtcgggacc taaggagaga ccgaaaggtg tatccgatgg acaacaggtt
     1501 gatattcctg tactagagta tgtagtgatg gagggacgca gtaggctaac taaagcagac
     1561 gattggaaga gtctgtctaa gcagtgaggt gtgatatgag tcaaatgctt atatctgtaa
     1621 cattgagctg tgatggggag cgaagtttag tagcgaagtt agtgacgtca cactgccaag
     1681 aaaagcttct agcgtttaaa catactctac ccgtaccgca aaccgacaca ggtagtcgag
     1741 gcgagtagcc tcaggtgagc gagagaactc tcgttaagga actcggcaaa atgaccccgt
     1801 aacttcggga gaaggggtgc tgactttaag tcagccgcag tgaataggcc caagcaactg
     1861 tttatcaaaa acacagctct ctgctaaatc gtaagatgat gtataggggg tgacgcctgc
     1921 ccggtgctgg aaggttaaga ggagtgctta gcgtaagcga aggtatgaat tgaagcccca
     1981 gtaaacggcg gccgtaacta taacggtcct aaggtagcga aattccttgt cgggtaagtt
     2041 ccgacccgca cgaaaggcgt aatgatttgg gcactgtctc aacgagagac tcggtgaaat
     2101 tttagtacct gtgaagatgc aggttacccg cgacaggacg gaaagacccc atggagcttt
     2161 actgcagttt gatattgagt gtctgtacca catgtacagg ataggtagga gtctaagaga
     2221 tcgggacgcc agtttcgaag gagacgctgt tgggatacta cccttgtgtt atggccactc
     2281 taacccagat aggtgatccc tatcggagac agtgtctgac gggcagtttg actggggcgg
     2341 tcgcctccta aaaggtaacg gaggcgccca aaggttccct cagaatggtt ggaaatcatt
     2401 cgcagagtgt aaaggtataa gggagcttga ctgcgagagc tacaactcga gcagggacga
     2461 aagtcgggct tagtgatccg gtggttccgt atggaagggc catcgctcaa cggataaaag
     2521 ctaccctggg gataacaggc ttatctcccc caagagttca catcgacggg gaggtttggc
     2581 acctcgatgt cggctcgtcg catcctgggg ctgtagtcgg tcccaagggt tgggctgttc
     2641 gcccattaaa gcggcacgcg agctgggttc agaacgtcgt gagacagttc ggtccctatc
     2701 cgtcgcgggc gtaggaaatt tgagaggatc tgctcctagt acgagaggac cagagtggac
     2761 ttaccgctgg tgtaccagtt gtcttgccaa aggcatcgct gggtagctat gtagggaagg
     2821 gataaacgct gaaagcatct aagtgtgaaa cccacctcaa gatgagattt cccatgatta
     2881 tatatcagta agagccctga gaga
//
LOCUS       DAVBGY010000103         2569 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVBGY010000103 DAVBGY010000000
VERSION     DAVBGY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.103"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            76..993
                     /locus_tag="VQ620_001990"
                     /pseudo
     CDS             76..993
                     /locus_tag="VQ620_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822901.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(1033..1977)
                     /gene="manA"
                     /locus_tag="VQ620_001991"
     CDS             complement(1033..1977)
                     /gene="manA"
                     /locus_tag="VQ620_001991"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HET2764564.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTILVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVESWTLEGQDLELIVSHP"
     gene            complement(2109..2390)
                     /locus_tag="VQ620_001992"
     CDS             complement(2109..2390)
                     /locus_tag="VQ620_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET2764565.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            2455..>2569
                     /locus_tag="VQ620_001993"
     CDS             2455..>2569
                     /locus_tag="VQ620_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET2764566.1"
                     /translation="MKSTKEEIQTIKTLFKDSRTAKYHKCLQIILFRLMGKS"
BASE COUNT          683 a          596 c          470 g          820 t
ORIGIN      
        1 gtgcatcagt tagttgttta cttgcttcat cattcataga actactatgc catattttgt
       61 ttcgcaggaa gtctgatcat ttcaaatcca gccattgctc taggagctca agcgaccttt
      121 atcctattga atatcttcat tgtatcacgt ggggttcaaa aagggattga aagagcttcg
      181 aaagtcatga tgcccctgct ctttatcatc tttgtcgtca tcatcgaacg ctctctcagt
      241 ttgccaaatg ccatggaagg ggttctttac ttcctcaaac cagatttttc aaaactgact
      301 agcgctggtc tcctctatgc tctgagacaa tctttctttg ccctctcact aggggttaca
      361 gccatgttga cctatgcttc ttacttggac aagaaaacca atctggtcca gtcaagaatc
      421 tccatcgtag ccatgaatat ctcggtatcc atcatggcag gtctagccat tttccaagct
      481 cggtccccct tcagactgaa tattgaaggg ggaccgagct tggtctttat cgtcttgcct
      541 caactctttg acaagatgcc ttttggaacc attttctatg tcctcttcct ctttgcgact
      601 gtcacttctt ctgtcgttat gctggaaatc aatgtagaca atatcaccaa tcaggacaac
      661 agcaagcgtg ctaaatggag tgttatttta ggaattttga cctttgtctt tggcattcct
      721 tcagccctat cttacggtgt catggcggat gttcacattt ttggtaaagc cttctttgac
      781 gctatggact tcttggtttc caatctcctc atgccatttg gagctctctt cctttcactt
      841 tttacaggct atatctttaa aaaggctctt gcaatggagg aactccatct cgatgaaaga
      901 gcatggaaac aaggactgtt ccaagtctgg ctcttccttc ttcgtttcgt cattccaatc
      961 atcatcgtgg tcttcatcgc ccaatttatg taatcaaaag gacttgagta atgaactcag
     1021 gtcctttctt ttttatggat gactaacaat taattctaaa tcttgccctt ctagagtcca
     1081 agattcaaca tcacttggta ggataaagtg tctccctttt tggattggat agtttttccc
     1141 gtcaacagtt agttgacctt gaccagccaa gacactcaac aagctgtagt cagctgtctt
     1201 ttcaaagtta acttttccag taatttccca tttgtaaact gcgaagaaat cattggatac
     1261 aaggatagtg gaacgtagat catctgcttt gattgtcact ggacggctat ttgcgggttc
     1321 accaatgttc aagacatcga tagatttttc aaggtgaagt tcacgtaaat tgcccttgtc
     1381 atccttacgg tcaaagtcat agacacggta agtggtatcg ctagactgct gggtttcaag
     1441 aatcaagata cccgccccga tagcgtgcat ggtaccgctt ggtacataga agaaatctcc
     1501 tgctttaact ggcacttttg tcaacagcgc atcccagttc ttatcctcga tttgctggcg
     1561 gagttcttct tttgacttgg cattgtgacc gtagataatc tctgaacctt catctgctgc
     1621 aatgatgtac caacattctg tttttccaag ttcgccttca tgctcgagtc cataagcatc
     1681 gtctgggtga acttggacac tgagccagtc gttggcatcg aggatcttgg tcaaaagtgg
     1741 aaatacaggt tctggacgat tgccaaataa ttcacggtgt tccgcataca aagtagcaag
     1801 atctgttccc tcgtaacgac cattggcaac tttagaaaca ccatttagat gagctgagat
     1861 agcccagtac tctccgactt tttcacttgg gatgtcgtag ccaaactcat cacgtagctt
     1921 ggtaccaccc cagatttttt cttgcataac tgattgtaaa aataatggtt ctgacatctt
     1981 aatctcctgt ctgatttttc tcccctcatt atagcaaaaa aagagttcca tttgaactct
     2041 tttttacatc ttataaagca gggagaagat tttataaaaa tagtaaacaa atgtgctcta
     2101 ctcgatgctt acaccattgc tataaatgac atgtaccaat agaaggactt cttcttgcta
     2161 cgtttgagag tcccatttcc tacattatct cgatctacat agataaagcc atagcgctta
     2221 ttcatttccc ctgtgccagc tgaaaccgaa tcgatacagc cccatgtcgt ataaccaagc
     2281 aagtcaaccc cgtctttgta aatggcatct cgcatggcct tgatgtgggc ctctaagtaa
     2341 gtaatccgat agtcatctgc tacataacca ttctcatccg gtgtatccat agcaccgagt
     2401 ccattttcta cgataatact atactaaaat caaaaagcat tatataatag tgatatgaaa
     2461 tcaactaaag aagaaatcca aaccatcaaa acacttttta aagactctcg tacagctaaa
     2521 tatcataaat gccttcaaat cattctattt cgtctgatgg gcaaatctt
//
LOCUS       DAVBGY010000104         2561 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVBGY010000104 DAVBGY010000000
VERSION     DAVBGY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.104"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..571
                     /locus_tag="VQ620_001994"
     CDS             <1..571
                     /locus_tag="VQ620_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2764567.1"
                     /translation="SYYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIA
                     QKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNP
                     YGWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKE
                     YLIPLPPLPEQSRIVDKIEQFFAHIDALI"
     gene            628..1425
                     /gene="psrA"
                     /locus_tag="VQ620_001995"
     CDS             628..1425
                     /gene="psrA"
                     /locus_tag="VQ620_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HET2764568.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            1436..2041
                     /locus_tag="VQ620_001996"
     CDS             1436..2041
                     /locus_tag="VQ620_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2764569.1"
                     /translation="MVKLTTCYFCDYGSYYGNIPMNWVVIKIKDIFSMNTGLSYKKGD
                     LSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHI
                     GKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQA
                     LYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQLWK"
     gene            complement(1989..>2561)
                     /locus_tag="VQ620_001997"
     CDS             complement(1989..>2561)
                     /locus_tag="VQ620_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2764570.1"
                     /translation="LLLYGNKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEA
                     TFWGTEIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVA
                     ILDIPATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISE
                     LLIPISNHEEMKRIISKVDLLFQKVSQLFE"
BASE COUNT          877 a          368 c          441 g          875 t
ORIGIN      
        1 ctcttattat gaggaagtac cttgtgaaat accagaaagt tgggaatggg tgaggttaaa
       61 cgatataacc tcctacattc aaagagggaa gtctccaaaa tattcaaata ttcctatata
      121 ccctgtgatt gctcagaaat gtaatcaatg gtctggcttt tctattgact tagcaagatt
      181 tattgatccc gaaacagttc atagttacca gaaagaaaga ttactaagag atggtgattt
      241 aatgtggaat tcaacaggtc taggtacgtt aggacgatta gctatttacc atgaaaataa
      301 aaatccttat ggctgggctg ttgcagatag ccatgttaca gttataagag tcttatcggg
      361 agttattaat tgccatttca tctataattt tctttcctct cctattgttc agtcagttat
      421 tgaagaaaaa gcgtcgggta gtacaaaaca aaaagaatta ttaacgaaaa ctataaaaga
      481 atatttaatc cccctcccac cccttcctga acaatccaga atcgttgata aaatcgaaca
      541 atttttcgcc catattgacg cactaattta gtataatagt tttacgtgcg gtggaatttc
      601 tatcaaacat aagataggag ttttacgttg aaagattttg aattacattt gaagaaagct
      661 ggtttagctg aaaatactgt gcgttcgtac ctttatggtg ttcgattttt cttggagaat
      721 tacgaactca aaatggaaga tttatttgaa tacaaaggat atttattaga caattttaaa
      781 cctaaaactg tcaatttgcg actacaagga gtaaataaat atctggcttt tattggtcat
      841 gatgatttga aattaaaatt tgtcaaagta cagcaaaaac cgtttttaga agatgtcatt
      901 agccatgctg attacctttt tcttaaacgt agtttgaaaa aagatggtat tttaaaatgg
      961 cattttgtcg tttggttttt aggtgcaact ggggctcgtg taagtgaact tattaaacta
     1021 aaagtagagc atgtagaaat cggttatttt gacatttact caaaaggtgg taaaattcgt
     1081 agactgtaca ttcctaaaaa attaagaaat agttgtctta gttggttaga atcagaaaat
     1141 cgccgaagtg gttatttatt tttgaataaa tttaacgaac caattacagc aagaggagtt
     1201 gctcaacagt taaaaaatta tgctgataaa tacaaaatga atcctaaagt aatttaccct
     1261 cattctttta ggcatttatt tgctaagaat tttttagcga agtataatga tattgccttg
     1321 cttgcagatt tgatgggaca cgaaagtata gaaactactc gaatttatct aaggaaaaca
     1381 gctactgaac aacaaaatat tgtagataaa attgttaatt ggtaaaaaat aacaggtggt
     1441 caaactgact acctgctatt tttgtgatta tggctcttat tatgggaata tacctatgaa
     1501 ttgggttgtt ataaaaataa aagatatttt ttcaatgaat acaggtcttt cttacaagaa
     1561 gggcgattta agcattaata ataaaggtgt tagaattata cgtggtggta atattaagcc
     1621 tttagaattt tctctgttgg ataacgatta ctacattgat acacaattca tctcctctga
     1681 acaagtttat ttaaaacata atcagctaat aacacctgta tcaacctctt tagaacatat
     1741 tggaaagttt gcaagaatcg ataaagacta tgatggtgtt gtggctggtg gatttatttt
     1801 ccaattaaca ccattcgaaa gttcagagat tatttcaaaa tttctattat ttaacttgtc
     1861 ttctccgtta ttttataaac aattgaaagc aataactaaa ctatcaggtc aagctttata
     1921 taatattcct aaaactacac tgagcgagct attaattccg ttagctcctt ttgaggaaca
     1981 ggaacttatt actcaaaaag ttgagaaact ttttgaaaaa gtaaatcaac tttggaaatg
     2041 attcttttca tctcttcatg attagaaata gggattaata attcggagat actggtacta
     2101 tttaatgttt tccctttgat agcatctttt gaatcaccta aagtagagat aagtggcaaa
     2161 aatatcatta agtaatctct gataatattt tctttattag cataggggaa tatcgatata
     2221 atggcttcat tatgagtggc aggaatatcc aatatggcaa cttttccaat agataattta
     2281 aaactcatta ataaagttcc tttaggtgaa atgtctattt tctttgattt taatgctaat
     2341 ttagaaatag attctctcgt attagttaca taaccagata taggcatatc tgatatagat
     2401 acccaaggta tttcagttcc ccaaaaagta gcttcactgc gtggaggagt ttttcctatt
     2461 ctgaagttaa ctaggctagc aaatttaata tatctccatg cttctgggat ttcatatata
     2521 ggataagagg ttgtttcgtc tttattccca taaagtaaga g
//
LOCUS       DAVBGY010000105         2361 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVBGY010000105 DAVBGY010000000
VERSION     DAVBGY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.105"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(62..1537)
                     /locus_tag="VQ620_001998"
     CDS             complement(62..1537)
                     /locus_tag="VQ620_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET2764571.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            2244..>2361
                     /locus_tag="VQ620_001999"
     CDS             2244..>2361
                     /locus_tag="VQ620_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2764572.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGK"
BASE COUNT          717 a          518 c          427 g          699 t
ORIGIN      
        1 tgccgtgctc taaaatcatc gccaaatcat caaattgaaa taagaaatag tttagataaa
       61 actattgact tttcttatag tttgtctttc tcttaataac gttgatatga ttgacctctg
      121 gatcttccgt cccttggagg gaacaaccac gttccttggc gaattggata tagtctacaa
      181 tttctcgtgt aatacgttca ccaggagcca agataggaat ccctggagga tagcacatga
      241 caaattctcc acagacctgt ccaacagact catctaaggt caaacttttt ctttctgaat
      301 agaaggcttc ttgcggagac agcactaact cgggctgaat atattctcct gctatcaaat
      361 cttttccatc tcttgaatag agtctcttaa tatcagccag agcaccaacc aagcgctcga
      421 tgtcttggat gcggtcgccg atggaaatat aggccaagat attgccgata tcaccaaact
      481 cgatctgaat gtcgtattcg tctcgcaaga ggtcataaac ctcgatacct gttaagccta
      541 ttccctgagt gtaaactgac aacttggtta catcaaaatc acagaccgac acaccatcta
      601 ttaactcttt tgagtaagca tagtaaccgc caatggcatt gatttcatga cgagcgtact
      661 cggatagctc aatgacttcc tcaaacgact ctttaccacg aagggccaag ttgcgacgtg
      721 aaatatccaa actagccatc aataagtaag aggcagatgt agactgggtc aggtttataa
      781 tctgacgaac gtactcggga ttcatctgct ccccgattaa aagaagcgaa ctttgtgtca
      841 aactcccacc agacttatgc atggaaactg ctgccatatc agctcctgca tccatagcag
      901 aaattggaag tttaccagtg aaatgcaaat gcgctccatg ggcttcatct actaaaacca
      961 tcatgccagc ttcatgagcc atttctgtca acccctttag gtctgaacag atgccgtagt
     1021 aagtaggatt gttgattagg atagccttag catctggatg gtcctttatg gcctgtgcta
     1081 ctcggtcatt ttcaagacct aaagcgatac caatcttagg atctacactc atctcgatat
     1141 agatgggaat ggcaccacat agaaccaacg cattgatagc agatttatgg acatttcgtg
     1201 gcagaataat cttatctcct gccttgcagg ttgccagaat catagtctgc accgatgaag
     1261 ttgttccacc aatcattaga aaggcatggc tagctccaaa agcatctgca gccagctcct
     1321 ctgcatcacg aataatcgaa ataggatggc ctaaattatc caagggtttc atcgaattga
     1381 catcaatgcc tacacatttt tctcctaaga gttcgacaag ttctggattt ccccgtccac
     1441 gcttgtgacc tggaacatca aagggaacaa tccttttctt gcgtaacttc accaaggcct
     1501 cataaattgg ggcttggttt tgatctaact ctttcaaaac atactccttt caatattttt
     1561 aggaccttat gaaaactaag gaactaaagg tggactcatg aaaaaagagc aggttttcac
     1621 ctgctctgca atcttctgac gtgtttattt tggtttctgt tgagtatgtc tgttcccgat
     1681 gtttcctaac tctctagtag caaatattac gtactttatt gatcgtttca caagatgacg
     1741 tgtgctttca ccacactaaa aatttatgaa ttagaacaag tgtcctaaca tcaacttata
     1801 aaagtaggct tttaacccta tcaataatgt ggcttttcaa ccacgcttcg gcattcaacc
     1861 ctgcctgtcc gtaggcattt tttactcggg tttatatttg ctagaaaaca tcatctcatt
     1921 ttctaagctt tattactata tcatgttttt aagaacttgt aaagtatttt ttgataaaaa
     1981 ataaaaatat agtagattga aactagaata gtacacctct gcttctaaaa cattgttaga
     2041 aatagatttt actgtcctga tcgatttgtt ctgttcttgt ttcattttac tatatgttcg
     2101 taaatgaaag ttctataatg aagttagcca cccagccctc ctaaaaatta gatggttcaa
     2161 ctagccgata gcttttgtag gcataagaaa acacctctgt gttacacttg ttgttcaacc
     2221 acaaacacaa gaaaggcaca gagatgcaag acaattatac tacaaaagcc aaacatttga
     2281 caatcgatag ccgtcgctta atcgaaagat ggaaaaaaga agggaaatca aatagagaaa
     2341 ttgcctctct acttggaaaa g
//
LOCUS       DAVBGY010000106         2215 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVBGY010000106 DAVBGY010000000
VERSION     DAVBGY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.106"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            101..1078
                     /locus_tag="VQ620_002000"
     CDS             101..1078
                     /locus_tag="VQ620_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2764573.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYKTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            1075..2157
                     /locus_tag="VQ620_002001"
     CDS             1075..2157
                     /locus_tag="VQ620_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2764574.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILTVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
BASE COUNT          700 a          500 c          402 g          613 t
ORIGIN      
        1 aaagctaata gcttatttga aattttctcc tctctgttgg atagtcccgt ctatctatgt
       61 tataatgaaa gaaggattta aaatcggaaa aggagtattt atgctaaaat taggtgtcat
      121 cggaactggc gctatcagcc atcatttcat agaagcagcc cataccagtg gagaatacca
      181 actggtcgca atctattcta gaaaactaga aactgcagca acctttgctt ctcgctatca
      241 gaatatccaa ctattcgatc aattagaagt cttcttcaaa tcttcttttg atttagtcta
      301 tatcgccagt ccaaactcct tacattttgc tcaggcaaaa gctgccttgt ctgctggtaa
      361 acatgttatt ctcgaaaagc cagctgtcac tcaaccacaa gaatggtttg atttgattca
      421 aacagctgaa aaaaataact gttttatctt tgaagcagct cgtaattacc acgagaaagc
      481 ctttactact atcaaaaact ttttagcaga taagcaagtg ttaggagcag atttcaacta
      541 tgccaaatac tcttccaaga tgcctgactt gttggctgga caaacaccaa atgtcttttc
      601 agaccgtttt gctggtggag ccctcatgga tttggggatt tatcccctct atgctgctgt
      661 tcgtcttttt gaaaaagcta atgacgcgac ttaccatgct caacagcttg acaatagcat
      721 tgacctaaat ggagatggta tcctcttcta cccagactat caagttcaca tcaaggctgg
      781 aaaaaacatc acttccaatc ttccttgcga gatttataca acagatggaa ccttgactct
      841 caacacgatt gagcatattc gctcagctat ttttaccgac caccaaggaa atcaagtcca
      901 gcttcctatc caacaggctc ctcatacgat gactgaggaa gtcgctgcat ttgcacacat
      961 gatccagcaa ccagatctga atctctacaa aacttggctg tatgatgcag gctctgttca
     1021 tgagctacta tataccatgc gccagactgc tggcattaga tttgaggcag aaaaatgaaa
     1081 accaaactac cgactgaatg gcaagaactg agtgaccaac tcggtttcca agaattcacc
     1141 cccattcaaa ctcaactatt tgagcccctt cttgctggag aaaacctcct aggagtgagc
     1201 caaaccggaa ctggtaagac cctagcgtat ctcctaccaa gtcttctcag actacaaaag
     1261 aaaaaagccc aacaactctt gattctagca ccaaacacgg aacttgctgg acagattttt
     1321 gatgtatgta aaacgtgggc agaagctatc ggattgactg ctcaactctt cctttcaggt
     1381 tcaagtcaga aacgacaaat tgaacgtctt aaaaaggggc cagaaattct gattggaact
     1441 cctggccgta tctttgaact cattaaactt aaaaaaatca agatgatgaa tgtggaaacc
     1501 atcatcctgg atgaatttga ccaattacta gatgattctc agattcactt tgtagaaaaa
     1561 atcactcact acgcacctcg tgaccaccaa ctggtctaca tgagtgccac gaccaagttt
     1621 gaccaagaaa agattgtgcc gaatacacgc actattgatc tctctgacca aaaattggac
     1681 aatatccagc atttctacat gcaggtagac caacgtcatc gagtggatat gctacgaaaa
     1741 ctggctcatg tagaggattt ccgtggtcta gtcttcttca atagcctgtc agaccttggg
     1801 aatgccgagg aaaaactaca gtatcgtgat atattgactg tttccctcgc tagtgatgtt
     1861 aatgttaaat ttagaaaaat catcttagaa aagtttaaag ataaccaact aaccctgctt
     1921 cttgcaactg accttttggc tcgtggaatt gatatcgata gcctagaatg tgtcgtaaac
     1981 tttgatattc ctagagatag cgaaacctac acacaccgtg ctggccgtac tggccgtatg
     2041 ggcaaggaag gatatgttat tactctcgtc actcatccag aagaaatcaa aaaacttaaa
     2101 aagtttgcaa gtatccgaga aattgtccta aaaaaccaag aactctatat caaataaagc
     2161 cccattgctc tatacacatc cagtgtatgg agctttttta taagcaattc aattt
//
LOCUS       DAVBGY010000107         2206 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVBGY010000107 DAVBGY010000000
VERSION     DAVBGY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.107"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(275..883)
                     /locus_tag="VQ620_002002"
     CDS             complement(275..883)
                     /locus_tag="VQ620_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HET2764575.1"
                     /translation="MQIKFIDKVSNLIMLNLLYVASVVTVIAIGSGESALIATLIKIV
                     RHEESYPYRDFANSFFKDYWKNLGAALISNLPILILLFSLFFLPYIPLPIYIISILRH
                     IGVIYIILHLIATTFLIPLIGRYNNTLKNSLHNSIMLAYKHFFIAVLIRIIEIIPVLL
                     FFILQNQLLVWITLMIFILPSITKYANAFLYNFIFSKYEKLN"
     gene            1049..1219
                     /locus_tag="VQ620_002003"
                     /pseudo
     CDS             1049..1219
                     /locus_tag="VQ620_002003"
                     /inference="COORDINATES: protein motif:HMM:NF013577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            1208..1819
                     /locus_tag="VQ620_002004"
     CDS             1208..1819
                     /locus_tag="VQ620_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2764576.1"
                     /translation="MTKDILELESQKMSSDTFIDEIKNNYLSIVESTRKLIDGRQIEL
                     AIKLIREANQILMIGVGSSGNAAREFESSLLRIGIISKTVIDTHFQLMHTALLKDNDL
                     IIAFSLSGSTKEVEETLLNAKRKNVKIISITNYSSRNIAKLSDCVLLTSKKESYLEGG
                     SLMAKASQLFIIDVICTRLSLINYEDTICKKEEIASLLSNKVE"
BASE COUNT          860 a          226 c          337 g          783 t
ORIGIN      
        1 acttagttct gtgcagtaca gaactaaatc ctttggataa attatttgtc taattttttg
       61 gggtcagtac agtattaaat accatgatct tatgcgtttt ataataaaaa atctgttctt
      121 gttttatttt tagaaaattt actaaagact aaaaatatta tgaataattt aaaaaatagt
      181 caggttatat agttttttga aattaaaatt attttatcgt tgaaattagt taaaaatttt
      241 tataaaatca agatgcaagg ctatgagatg ttctctagtt taatttttca tacttcgaga
      301 atataaagtt atataaaaat gcattagcat attttgttat tgagggaagt ataaaaatca
      361 ttaaagttat ccaaactagt aattgattct gtaatataaa gaagagtaga actgggataa
      421 tttcaataat tctaataaga acggcaataa aaaaatgttt ataggctaac atgattgaat
      481 tgtgtagaga attttttaaa gtgttattat atctaccgat taaaggaatt agaaatgttg
      541 ttgctattaa atgaagtatg atatatatta ctccaatatg tcgtaagata gaaatgatat
      601 atataggtaa tggtatgtag ggtaggaaga acaaactgaa taataagatt aaaattggta
      661 aattagatat taatgctgct cctaaatttt tccaataatc tttaaaaaaa ctgtttgcga
      721 agtctctata gggataggat tcctcgtgtc gtacaatttt tattagagtt gctattaggg
      781 cactttcacc tgatccgata gcaataactg ttactactga cgctacatag agtagattta
      841 acattataag atttgaaact ttatcaataa attttatctg catctttaac tcctgctgtt
      901 tttatactta taatatcaca tcatttgagt ttcatcaact ttgaatacat taagaaagaa
      961 tataactatg aggaaaggaa aaaattatta tgaataaatc caaaaaatat aaagaaagaa
     1021 ttcattatga atacgataat cttactaaat cagagaaaaa attgctgact atattttagt
     1081 tagagatagt gaaatagttt atgaaacgtc ttcagaaatt gttagtaaag taaaagttgg
     1141 agagggaact cttaatcgat tttgcaaaaa gattggatac tctggttttc aggaactaaa
     1201 attaaaaatg acgaaagata tattagagtt agaatctcaa aaaatgagta gtgacacatt
     1261 tattgatgaa ataaaaaata attatttatc tattgtggaa agtacaagaa aattaattga
     1321 tggtagacaa atagaattag ctataaaatt aataagagaa gcaaaccaga ttttaatgat
     1381 tggtgtgggt tctagtggaa atgcggctag agaatttgag agtagtttgt taagaattgg
     1441 aataataagt aagacggtaa ttgataccca ttttcaactc atgcatactg cgttattgaa
     1501 agataatgat ttgataatag cgtttagttt atctggttcc acaaaagaag ttgaggaaac
     1561 tttattaaac gcaaaaagaa aaaatgttaa aattatttca ataacaaatt attcgtccag
     1621 aaatatagca aaattatctg actgcgttct tttgacatca aaaaaagaat cttatttaga
     1681 gggtggtagt ttaatggcta aggcttctca actattcata attgatgtaa tatgcactag
     1741 actctcttta ataaattatg aagatactat atgtaaaaaa gaagagattg cttctttgct
     1801 gtcaaataaa gtggaataaa tcaataattt ttaaatttta ataaaaattt tttaaaataa
     1861 attaaaaaac gatcctaaaa gggtcgtttt tttgctaaag ttttaaaaat aaacaataat
     1921 taagattata gtgcgttgaa tctatattag gacataacaa ttcttaaatc attgttagaa
     1981 attagtttga attttccaat caatttgttc gatttttaca tcattgcatt atatcacgtg
     2041 actattaacc ttgttttctc ctaaaattga ttttcttctt ttcttatctt gtccactcga
     2101 aacaagtatt gtaagaattt gattattttt gaaagtactt ttaatatatt tgagatagtt
     2161 aaaaaagatt tgaaactaaa ttccaaatta gaaaaagact tgaaat
//
LOCUS       DAVBGY010000108         2181 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVBGY010000108 DAVBGY010000000
VERSION     DAVBGY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.108"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            368..1714
                     /gene="gdhA"
                     /locus_tag="VQ620_002005"
     CDS             368..1714
                     /gene="gdhA"
                     /locus_tag="VQ620_002005"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HET2764577.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          695 a          390 c          407 g          689 t
ORIGIN      
        1 ttggatttct tctttagttg atttcatatc actattatat aatgattttt gattttagtc
       61 tggtataaat attgctttcc tccaaaatgg tcataatttt actagcaaat ctaacatatc
      121 acggataaat taacaagtga tttctgaatt gctaaacatt ttcttttctt atagcatacc
      181 atactttaag attttgtctt tgagaaagat atttccaaga aaaacgttcg tttttttatc
      241 tattttcaaa aaaaccaaaa actgaattga aagaattttt agaaaatttc tgttttttct
      301 ttacagtgaa aaaaaattct gctataatgt cttatgtaat aaaatatgac aataaaagga
      361 gaacgatatg acatctgcta aagaatatat ccaaagcgtg tttgaaactg tgaaagctcg
      421 taacgggcac gaggctgaat tcctccaagc tgttgaagaa tttttcaaca ctttagaacc
      481 tgtatttgaa aaacaccctg agtatatcga agaaaatatc ttggcacgta ttactgagcc
      541 tgagcgcgtg gtttctttcc gtgttccttg ggttgaccgt gatggaaaaa ttcaagtaaa
      601 ccgtggttac cgtgttcaat tcaactcagc tgttggacca tacaaaggtg gacttcgttt
      661 ccacccaact gtaaaccaag ggatcttgaa attcctcgga tttgaacaaa tctttaaaaa
      721 tgtcttgact ggacttccta tcggtggagg taaaggtgga tcagacttcg atcctaaagg
      781 taaaacagat gctgaagtga tgcgcttctg ccaaagcttc atgactgaat tgcaaaaaca
      841 catcggacca tcacttgacg tacctgctgg tgatatcggt gttggtggac gtgaaattgg
      901 ttacctttac ggtcaataca aacgtcttaa ccaatttgat gctggtgtct tgactggtaa
      961 acctcttgga tttggtggta gcttgattcg tccagaagca actggttacg gtttggttta
     1021 ctatactgaa gaaatgctca aagctaacgg taacagcttt gctggtaaga aagtcgttat
     1081 ttcaggttct ggtaacgttg ctcaatatgc tcttcaaaaa gcaactgaac tcggtgcaac
     1141 tgttatttct gtttctgact caaatggtta tgtcatcgat gaaaatggta tcgacttcga
     1201 tcttttggtt gatgttaaag aaaaacgtcg tgctcgtttg actgagtatg cagctgaaaa
     1261 agcaactgca acctaccacg aaggtactgt atggacttac gctggaaact atgacattgc
     1321 tcttccatgt gccactcaaa acgaaatcaa cggtgaagca gctaaacgtt tggttgctca
     1381 aggcgttatc tgtgtatctg aaggtgccaa catgccgagc gaccttgatg ccatcaaagt
     1441 ttacaaagaa aatggtatct tctacggacc tgcaaaagct gccaacgctg gtggtgtagc
     1501 cgtttcagct cttgaaatga gccaaaacag tcttcgcctc tcatggactc gtgaagaagt
     1561 tgatggccgt ctcaaagaca tcatgacaaa catcttcaac acagctaaaa cgacttcaga
     1621 aacatacggt cttgataaag actaccttgc aggagctaac attgctgcct ttgaaaatgt
     1681 agcaaatgcc atgattgcac aaggtattgt ttaagattta tttactcaat cctcaatcac
     1741 tacgattggg gatttttagc ttgaattaaa aaattggaaa atctaattaa ataaaatgaa
     1801 atgaaataaa aactgaacaa attgattgcg gaattcaaac caatttctaa catacttcta
     1861 gaaattaatt tgacttttct aatcgatttg ttcatatctt atttcaattt actatactta
     1921 cttaaccctt tttatactca atgaaaatca aagagcaaac taggaagcga gccgcaggct
     1981 gtacttgagt acggcaaggc aaagctgacg tggtttgaag agattttcaa agagtattaa
     2041 acaaaaagaa aaaccgctac tcctaagaat agcgatttaa tcatgttttt aagctactac
     2101 tgtagttgct ctattattta agtgttatga ataacttcaa tatctttaaa gctttttgtt
     2161 agatttataa tttcaactat a
//
LOCUS       DAVBGY010000109         1956 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVBGY010000109 DAVBGY010000000
VERSION     DAVBGY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.109"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            288..1833
                     /locus_tag="VQ620_002006"
     rRNA            288..1833
                     /locus_tag="VQ620_002006"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1885..1956
                     /locus_tag="VQ620_002007"
     tRNA            1885..1956
                     /locus_tag="VQ620_002007"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1918..1920,aa:Ala,seq:tgc)
BASE COUNT          526 a          412 c          582 g          436 t
ORIGIN      
        1 cggcggtaac acgggttcga atcccgtacg gactatggta tgttgtggca ggaccacttg
       61 atgaaaaaaa gtttaaaaaa acttaaaaat cttcaaaaaa gtgttgacaa gtgaaagtgg
      121 ctgtgatata ctaatatagt tgtcgcttga gagaagcaag tgacaaagac ctttgaaaac
      181 tgaacaagac gaaccaatgt gcagggcgct acaacataag ttgtagtact gaacaatgaa
      241 aaaaacaata aatctgtcag tgacagaaat gagtgagaac tcaaactttt aatgagagtt
      301 tgatcctggc tcaggacgaa cgctggcggc gtgcctaata catgcaagta gaacgctgaa
      361 ggaggagctt gcttctctgg atgagttgcg aacgggtgag taacgcgtag gtaacctgcc
      421 tggtagcggg ggataactat tggaaacgat agctaatacc gcataagagt agatgttgca
      481 tgacatttgc ttaaaaggtg cacttgcatc actaccagat ggacctgcgt tgtattagct
      541 agttggtggg gtaacggctc accaaggcga cgatacatag ccgacctgag agggtgatcg
      601 gccacactgg gactgagaca cggcccagac tcctacggga ggcagcagta gggaatcttc
      661 ggcaatggac ggaagtctga ccgagcaacg ccgcgtgagt gaagaaggtt ttcggatcgt
      721 aaagctctgt tgtaagagaa gaacgagtgt gagagtggaa agttcacact gtgacggtat
      781 cttaccagaa agggacggct aactacgtgc cagcagccgc ggtaatacgt aggtcccgag
      841 cgttgtccgg atttattggg cgtaaagcga gcgcaggcgg ttagataagt ctgaagttaa
      901 aggctgtggc ttaaccatag taggctttgg aaactgttta acttgagtgc aagaggggag
      961 agtggaattc catgtgtagc ggtgaaatgc gtagatatat ggaggaacac cggtggcgaa
     1021 agcggctctc tggcttgtaa ctgacgctga ggctcgaaag cgtggggagc aaacaggatt
     1081 agataccctg gtagtccacg ctgtaaacga tgagtgctag gtgttagacc ctttccgggg
     1141 tttagtgccg tagctaacgc attaagcact ccgcctgggg agtacgaccg caaggttgaa
     1201 actcaaagga attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagca
     1261 acgcgaagaa ccttaccagg tcttgacatc cctctgaccg ctctagagat agagttttcc
     1321 ttcgggacag aggtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg
     1381 gttaagtccc gcaacgagcg caacccctat tgttagttgc catcatttag ttgggcactc
     1441 tagcgagact gccggtaata aaccggagga aggtggggat gacgtcaaat catcatgccc
     1501 cttatgacct gggctacaca cgtgctacaa tggctggtac aacgagtcgc aagccggtga
     1561 cggcaagcta atctcttaaa gccagtctca gttcggattg taggctgcaa ctcgcctaca
     1621 tgaagtcgga atcgctagta atcgcggatc agcacgccgc ggtgaatacg ttcccgggcc
     1681 ttgtacacac cgcccgtcac accacgagag tttgtaacac ccgaagtcgg tgaggtaacc
     1741 gtaaggagcc agccgcctaa ggtgggatag atgattgggg tgaagtcgta acaaggtagc
     1801 cgtatcggaa ggtgcggctg gatcacctcc tttctaagga taaggaactg cgcattggtc
     1861 ttgtttagtc ttgagaggtc ttgtggggcc ttagctcagc tgggagagcg cctgctttgc
     1921 acgcaggagg tcagcggttc gatcccgcta ggctcc
//
LOCUS       DAVBGY010000110         1860 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVBGY010000110 DAVBGY010000000
VERSION     DAVBGY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.110"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(3..968)
                     /locus_tag="VQ620_002008"
     CDS             complement(3..968)
                     /locus_tag="VQ620_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2764578.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKVQRLGIAGVEIRLRKLGDKLGIQKVPPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            complement(1086..1499)
                     /locus_tag="VQ620_002009"
     CDS             complement(1086..1499)
                     /locus_tag="VQ620_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HET2764579.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSPSALNMIVNLPEQ
                     FVFGKPLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWISEHSVREKVKK"
     gene            complement(1496..1732)
                     /locus_tag="VQ620_002010"
     CDS             complement(1496..1732)
                     /locus_tag="VQ620_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HET2764580.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
BASE COUNT          542 a          362 c          277 g          679 t
ORIGIN      
        1 ttttaagaga tgaatttttg gtgtgaatgc ttgacattat tttgattgac catggcatag
       61 gccagggttg tgtcaatctt gctatgacct agcagttttt ggacttgttc gataggcata
      121 cctttatcaa ttgccttagt cgctaaagtt cttctgaact tatgtggggg aaccttttgt
      181 atgccgagtt tgtctcctaa ctttcttaag cgaatctcta caccagcaat tccaagcctc
      241 tggacttttc caactagcgt tacaaaaaga gcagggtgac tatcttttct gtcgttaaga
      301 taatttctta aatgaatttt cgtacgagcg tcaaaatata ctggtctctc cttctttcct
      361 ttaccaaaga caacacactc tctgttttca aaatcaatat ctgaacgatt caactgtaca
      421 agctccccta cacgcatacc tgtcgatgct agtaggtcta ttattgccaa atctctcaaa
      481 ttttcacagt tatcacgcat aatttccaaa tgttcatcag tataagtttc cttcacattt
      541 tgctcagtct taattttctt tatccgtcga atgggatttt taatgatata ttcctcttgc
      601 tcaagccaag caaaaaaaga agacaatata cgcctaatat tgtctaaatt tgccttacta
      661 caatcctttt cagactggta atttgctaaa taactcctta aatcatctgt tgtgagttgt
      721 gtcacagact ctttaatagc attaaacaag ttttcaatcg tcctctgata ataacgaatt
      781 gtttttgagc tacagccctc tacctgcttg gcggtaataa atagttgcaa caggttatta
      841 ttcgaatact tctgctgctt ttttccgtct actagatgat gttctaaact ctctctcaac
      901 gctagtaatt gttcattatt taaaagaggt aacatttctt gcatgataat tgtaactttt
      961 gattccataa tttctcactt tcttattcat caataaaaag agacaatatc agtttctgca
     1021 ttacagaatt ctaatattgt ctgttcattt attttcttgt gctcattcaa atccagcata
     1081 ctaggttact ttttgacctt ttctctaaca gaatgttcag aaatccattt ggagtagctt
     1141 gtcatttttt catcagttaa agcttctact gcgatggtta cacacatttt tactgcttct
     1201 tctaccgtta cagaaaaacg atagccgttt aattgtaaaa atttgaccaa aacgaagaaa
     1261 gcagttcttt tattagcatt agcaaaaaca tgcttcttta tcaattggac aaatagtatc
     1321 gttgctttat caaaaattgt tggataaaga ggcttcccaa agacaaattg ttctggtaag
     1381 ttcacaatca tatttaaggc agaaggacta actgtttgaa ttttctcatt tggagaatac
     1441 cgttgaattg ctaaagcatt tattttttca atttgctttt ctgtcagata gattgtcatt
     1501 tttccaccaa agctttgaaa acatcgtcgt attcgtcaaa tattttatct aaatttaagt
     1561 caaaggcatc atcagataca taagaaacct gctgccccaa ttcttcagga gtaaagataa
     1621 tctcaccact tggtaacaat ttcgcttcgt atttaacacc acttggaata ttaaattcac
     1681 ttggaatcgt aatggtgatt gaatttcctt gttttcttgt ttttactacc atctgatttc
     1741 ctcctttgaa gtattataac aaatcattac cgtaattaca atggcagaat atcagccaaa
     1801 atactcctgc atcagagatt tcttcaaagt ttcaagttct tccagagatt tttggattgc
//
LOCUS       DAVBGY010000111         1859 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVBGY010000111 DAVBGY010000000
VERSION     DAVBGY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.111"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..260
                     /locus_tag="VQ620_002011"
     CDS             <1..260
                     /locus_tag="VQ620_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HET2764581.1"
                     /translation="QIKSALEEELSNRGYDVHCDAVMVKDVNEDLMKGYDIFTPIAAT
                     DLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            308..1783
                     /locus_tag="VQ620_002012"
     CDS             308..1783
                     /locus_tag="VQ620_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HET2764582.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
BASE COUNT          529 a          378 c          375 g          577 t
ORIGIN      
        1 accaaattaa aagtgctcta gaagaagaac tttcaaaccg tggttatgat gttcactgtg
       61 atgcagtcat ggtgaaagat gtaaacgaag accttatgaa aggttacgat atctttacac
      121 caatcgctgc aacagacctt ggttttgaac caggtatccc agttatcgaa gctggaccaa
      181 tcttattccg tatcccagca atgagcgctc cagtatttga caatattgaa gcagctatta
      241 aagaacatgg attaagctaa ttctaatttg ttaacattca tataacataa aaaagggagg
      301 aactgttatg gatgcaatct ttgacctaat cggaaaggtt ttcaatccca tcttagaaat
      361 gggtggacct gtcatcatgt tgatcatttt gacagtattg gctttacttt ttggagtgaa
      421 attctccaaa gcgcttgaag gtggtatcaa acttgccatc gctcttacag gtatcggtgc
      481 tatcatcggt atgctaaatg gtgctttctc agcatcactt gcaaaattcg ttgaaaacac
      541 tggtatccaa ttgagtatta cagacgttgg ttgggcacca ctcgcaacaa tcacttgggg
      601 ttctgcttgg acactatact tcttgctcat catgttgatt gtcaacgtag tgatgcttgc
      661 tatgaagaaa accgatacac ttgatgtcga tatctttgat atctggcact tgtctatcac
      721 aggtctcttg attaaatggt atgctgataa caatggtgtg agtcaagggg tttcactctt
      781 tattgctaca gcagctatcg tccttgtcgg tgtgttgaaa attatcaact ctgacttgat
      841 gaaacctaca tttgatgacc ttcttaacgc cccaagttca tcaccaatga catcaactca
      901 catgaactac atgatgaacc cagttatcat ggttttggat aagatttttg aaaaattctt
      961 cccaggcctt gataaatatg actttgatgc tgctaaattg aacaagaaaa tcggtttctg
     1021 gggatctaaa ttcttcatcg gtttcatcct tggtatcgtt atcggtatta tgggaactcc
     1081 acatccaatt gcaggtgttg cagatgcaga taaatggcgt cttgttatca aaggatggtt
     1141 gtctcttggt ttgactgccg gtgtatcttt ggaactcttc tcacttatcg gttcatggtt
     1201 catcgcagcc gtagaaccac tatcacaagg tattacaaac gttgctacta aacgtcttca
     1261 aggacgtaaa ttcaatatcg gtcttgactg gccattcatc gctggtcgtg ctgaaatctg
     1321 ggcttgtgcc aacgtacttg caccaatcat gttgattgaa gcagtgcttc tttcaaaagt
     1381 tggaaatggt atcttgccac ttgcaggtat catcgctatg ggtgttactc cagctctctt
     1441 ggttgtaact cgtggtaaat tgctccgtat gattatcttc ggaacactct tgttgccact
     1501 cttccttctt tcaggtacac ttattgcacc atttgcaaca gaacttgcta aaggtgtagg
     1561 tgccttccca gaaggtgtga gccaaactca attgattact cactctactc ttgaaggacc
     1621 aatcgaaaaa cttcttggtt ggacaattgg taacactaca actggtgata tcaaagcaat
     1681 ccttggtgca gtagtcttcc ttgtattcta tatcggtatc tttgcttggt acagaaaaca
     1741 aatgatcaaa cgtaacgaag agtacgcagc aaaagcaaaa taatgcgctc ctataaaata
     1801 taaacttaat gaaaatcaaa gagcaaacta ggaagctagc cgcaggttgc tcaaaacac
//
LOCUS       DAVBGY010000112         1650 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVBGY010000112 DAVBGY010000000
VERSION     DAVBGY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.112"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            107..406
                     /locus_tag="VQ620_002013"
     CDS             107..406
                     /locus_tag="VQ620_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HET2764583.1"
                     /translation="MDSYAINFWESKGVGIDKKILEFIQKNITVIKNKNFLNKDFDIY
                     VKKIHNLFFVISTLVSKEQYMGIVSSQIHMFANRMGISPTIEVTMLDFFLREDSD"
     gene            408..1178
                     /locus_tag="VQ620_002014"
     CDS             408..1178
                     /locus_tag="VQ620_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HET2764584.1"
                     /translation="MEKYFFDISIFEKIKKVLSYQSKYWEISKPRDKYSFKSQNRISG
                     SNSIKFKDFELDYELESLGKILLKRRSFLLNEFDKNVSFDKLIYLLKTVFSPTNCKFV
                     FGEEVPLFSYPTSGALNSITPFVYIDNMDEVKNGIYEVKYDGLEFKKSMNIIDLETFT
                     PITSKNSDVQNNSFKKVNMIIMLCSNYENIFNKYGLLAYRLLHLEAGHIAQNIQLVST
                     ALGIKSLPMGGWFDDKIETFINEISDKKNIYLYAIAVG"
     gene            1198..1362
                     /locus_tag="VQ620_002015"
     CDS             1198..1362
                     /locus_tag="VQ620_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2764585.1"
                     /translation="MNAEDKFRELENENKLLKLHNKELENEIQNGWKFWIWVTVPILG
                     LIYAIIQKIF"
BASE COUNT          607 a          180 c          269 g          594 t
ORIGIN      
        1 taactattat tacgaaaaaa aattcttgtg taaaattcta ttgactttct atattatttt
       61 tgtatttcat aacgaatgta atttaacaag tgaagaaatt ttaaagatgg attcctatgc
      121 tattaatttc tgggagagta aaggggtcgg tattgacaaa aaaatcttgg aatttattca
      181 aaaaaatatt acagttatta aaaataaaaa ttttttgaat aaagattttg atatttatgt
      241 taaaaagatt cataatttat tctttgtcat ttctacttta gtttcaaaag agcaatacat
      301 ggggatagtt tcttcccaaa tacatatgtt cgcaaatagg atgggaattt ctcccaccat
      361 tgaagttacc atgctagatt tttttctgag ggaggattct gattaatatg gaaaaatatt
      421 tttttgatat ttctattttt gaaaaaataa aaaaagtatt atcataccaa tctaaatatt
      481 gggaaatatc gaagcctagg gataagtaca gtttcaaaag tcaaaataga atatctggct
      541 ctaatagcat taaatttaaa gattttgagt tagattatga actggagtca cttggtaaaa
      601 tacttttaaa aaggagatcg tttttactaa atgaatttga taagaatgtg agttttgaca
      661 aactgattta cttgttgaaa actgtattct ctcctacaaa ttgcaagttt gtatttggag
      721 aagaggttcc tttatttagc tatcctacat caggtgcgtt aaattcaatt actccttttg
      781 tttatattga taatatggat gaagtaaaga atggtatata tgaagtaaag tatgacggac
      841 ttgaatttaa aaaaagtatg aatattatag acttagaaac atttacccca ataacaagta
      901 aaaacagtga cgtacaaaat aattcattta aaaaggtaaa tatgataatt atgttatgtt
      961 cgaattatga aaatattttc aataaatatg gattattagc ttatcgactt ttgcatttag
     1021 aggctggtca tattgcacaa aatatccagc ttgtttctac tgcgctagga ataaaaagtt
     1081 taccgatggg aggttggttt gatgataaaa tagaaacttt tattaatgaa attagtgata
     1141 aaaaaaatat ttatctctat gcaattgcag ttggataaat ttaaggagga agtgtagatg
     1201 aacgcagagg ataaatttcg cgaactagaa aatgaaaata aacttctaaa acttcataac
     1261 aaggagcttg agaatgaaat tcaaaatgga tggaaatttt ggatatgggt aactgtccct
     1321 attttaggtt tgatatatgc tattattcaa aaaatttttt aattatgcta gtttgtcagg
     1381 ttgagtagtt tttgttcaaa atttaataaa cttttttcaa tgaagattat ctaaagattg
     1441 cttctcagat gagaatggct tctctatctt atataaatta tcttcataga gtgtcctgtt
     1501 attcatgtta tcacagaata gatagttttt ttggctctat aatatttgta gtgggtaaat
     1561 cccctataga tattatggag cctatttttg tgtagaaaaa aagtcccata tcatctataa
     1621 tgaaaagcaa ccaaactatc attagaaaga
//
LOCUS       DAVBGY010000113         1643 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVBGY010000113 DAVBGY010000000
VERSION     DAVBGY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.113"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(119..1543)
                     /locus_tag="VQ620_002016"
     CDS             complement(119..1543)
                     /locus_tag="VQ620_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HET2764586.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMLKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
BASE COUNT          480 a          364 c          285 g          514 t
ORIGIN      
        1 atctgtacta ttctagtttc aatatactat aataccaaaa agttcgtatt ttttctataa
       61 ttattattat tttatagcaa aaaataaaac caggaaaaca atttcctgat tttacacgtt
      121 atttcacatc aaaaacaatg gatttgacat ttgtcatcgc ttcaatgcta tatttaattc
      181 cttgcactcc agctccagaa cctttgacac caaggaatgg gaaattatct ggaccacgct
      241 gggttttatt attaatgtgg actgtaccta cttcaagttt ttcagcaatt tcaaaggctt
      301 ttttgaaatc atttgtaaag actgatgatt gaaggccgaa ttcagattcg ttggcaaagg
      361 caatagcttc ctctacacta gccacacgaa tgattggtaa aacaggacca aatggctctt
      421 cccatgccac tttcatatct tttgtaactt ggtcaaaaag cactggccag aggagattgc
      481 cctcacgttt gattggtgta agagcctgag cctctttttc ttgtgcatcc tcaatcaagc
      541 cccaaatgaa gtcggctgaa gcattgtcaa taacaggtgt aatatcagca ttgtcaaatg
      601 gatcaccaac tgttaattta gaaacttctt cctgaagcaa agtagctaat ttatctgcta
      661 cactttcgag aacaatgaca cgtttaatgg ccgtgcaacg ttgtcctgag tagctaaagg
      721 ctcccgcaac aatttgcttg gcagcatgtt ccaaatctgc atcttctagt acaagagctg
      781 catctttccc accaagttcc aacatgatag gacgcatacc agctaaacga ccaatacgtt
      841 ctccaatagg agttgaacct gtaaagttga tgaagttgac ttctttgtgc tcaatgatat
      901 aatccccaat ttctgaacca cgacctgtaa tggtgttgaa aacacctgcc ggaatccctg
      961 cttcttcaaa tgctttagcc aacaagagtc cagaaatgga accttgtgtt ggtggcttca
     1021 acatgaccac attccctgca atcaaggcag gtgcaatttt agaagcagat aaattaactg
     1081 gataattaaa gggagcaatc gctagcacga taccaactgg ttcacgacgg acaacagcca
     1141 gtttgttttt acttgctgcc tcaaaaccac caccttccat tgcttgtcca gtgatacgga
     1201 gaccttcctc agcagcataa cgaatcaagt ctgctgtacg cactacttct ccaattgctg
     1261 ctttaatccc ttttgctact tctttggcaa ggatagtacc aattttttcc ttatcgcgtt
     1321 ctaaaatagc tgctgtttta tgcaaataag ccgcacgttc aattgctgat aaagctcgcc
     1381 atgctggcag ggctgcacgc gcagcttgca tagcctcatc agcttcagtc tgagtcatgg
     1441 ctggaactgt acccaattct tcttgattga ttggtgaata aatcgtaatt tcttgttcag
     1501 atgatttcca ttttccattt actaaattct gatatcttgt caaattcttg tcctcctgaa
     1561 aatgattaag atatatttta ccaaattttc agaaaataac aacccttttt gcaaaatttt
     1621 tgagattatt ttcacaagct tga
//
LOCUS       DAVBGY010000114         1581 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVBGY010000114 DAVBGY010000000
VERSION     DAVBGY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.114"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            141..>1581
                     /gene="pcpA"
                     /locus_tag="VQ620_002017"
     CDS             141..>1581
                     /gene="pcpA"
                     /locus_tag="VQ620_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HET2764587.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDGA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLWYYLNESGS"
BASE COUNT          618 a          212 c          255 g          496 t
ORIGIN      
        1 tgtgtttatg gagagatgac aatttataaa atgtactact ctattctact agataatgat
       61 tgaataaaac tttttataac aacggctagc aaagttataa ttttatatct attagaaaat
      121 aaatatgtaa ggaaatttaa atgaaaaaaa ctacaatatt atcattaact acagctgcgg
      181 ttattttagc agcatatgtc cctaatgaac caatcctagc agatactcct agttcggaag
      241 taatcaaaga gactaaagtt ggaagtatta ttcaacaaaa taatatcaaa tataaggttc
      301 taactgtaga aggtaacata ggaactgttc aagtgggtaa tggagttact cctgtagagt
      361 ttgaagctgg tcaagatgga aaaccattca cgattcctac aaaaatcaca gtaggtgata
      421 aagtatttac cgttactgaa gtagctagtc aagcttttag ttattatcca gatgaaacag
      481 gtagaattgt ctactatcct agctctatta ctatcccatc aagcataaaa aaaatacaaa
      541 aaaaaggctt ccatggaagt aaagctaaaa ctattatttt tgacaaaggc agtcagctgg
      601 agaaaattga agatggagct tttgattttt ctgaattaga agagattgaa ttgcctgcat
      661 ctctagaata tattggaaca agtgcatttt cttttagtca aaaattgaaa aagctaacct
      721 tttcctcaag ttcaaaatta gaattaatat cacatgaggc ttttgctaat ttatcaaatt
      781 tagagaaact aacattacca aaatcggtta aaacattagg aagtaatcta tttagactca
      841 ctactagctt aaaacatgtt gatgttgaag aaggaaatga atcgtttgcc tcagttgatg
      901 gtgttttgtt ttcaaaagat aaaacccaat taatttatta tccaagtcaa aaaaatgacg
      961 aaagttataa aacgcctaag gagacaaaag aacttgcatc atattcgttt aataaaaatt
     1021 cttacttgaa aaaactcgaa ttgaatgaag gtttagaaaa aatcggtact tttgcatttg
     1081 cagatgcgat taaacttgaa gaaattagct taccaaatag tttagaaact attgaacgtt
     1141 tagcctttta cggtaattta gaattaaaag aacttatatt accagataat gttaaaaatt
     1201 ttggtaaaca cgttatgaac ggtttaccaa aattaaaaag tttaacaatt ggtaataata
     1261 tcaactcatt gccgtccttc ttcctaagtg gcgtcttaga ttcattaaag gaaattcata
     1321 ttaagaataa aagtacagag ttttctgtga aaaaagatac atttgcaatt cctgaaactg
     1381 ttaagttcta tgtaacatca gaacatataa aagatgttct taaatcaaat ttatctacta
     1441 gtaatgatat cattgttgaa aaagtagata atataaaaca agaaactgat gtagctaaac
     1501 ctaaaaagaa ttctaatcag ggagtagttg gttgggttaa agacaaaggt ttatggtatt
     1561 acttaaatga atcaggttca a
//
LOCUS       DAVBGY010000115         1432 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVBGY010000115 DAVBGY010000000
VERSION     DAVBGY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.115"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            84..491
                     /locus_tag="VQ620_002018"
     CDS             84..491
                     /locus_tag="VQ620_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2764588.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKIG"
     gene            656..955
                     /locus_tag="VQ620_002019"
     CDS             656..955
                     /locus_tag="VQ620_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HET2764589.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVNEANIKTLYSEKLIGISIPIK"
     gene            1216..>1432
                     /gene="tnpB"
                     /locus_tag="VQ620_002020"
     CDS             1216..>1432
                     /gene="tnpB"
                     /locus_tag="VQ620_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET2764590.1"
                     /translation="MMIVLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRF"
BASE COUNT          492 a          281 c          253 g          406 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagc gaatacagac tatattgctc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gagaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact tctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tccaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattggcta atactcttcg aaaataaaat tcaaaccacg tcagcgtcgc cttaccgtac
      541 tcaagtatag cctgcggcta gcttcctagt ttgctctttg attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcaactag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag acctctgtta atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataaca aaacgcatag
     1021 taacaagagt ttctacaccc actattatac gtttttatca ttttgaaatc tgctgctaat
     1081 caatgaagaa gcaaagatca aactaggcag ctagttacaa gttacgtaag cgcgtcataa
     1141 caaggtatct atcctaattc cccaacttcc agtatactag ataaaaaata attatcagag
     1201 gaaaggtatt gtactatgat gattgtacta agtgatttag gtcaagttca ccttgtttgt
     1261 ggcaagacag atatgaggca gggaatagac tcattagcct atgtagttaa aacccacttt
     1321 gaattggatc ctttctccgg tcaagtcttt ctcttttgtg gtggacgtaa agaccgcttt
     1381 aaagcccttt actgggatgg tcaaggattt tggctactat ataaacgctt tg
//
LOCUS       DAVBGY010000116         1382 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVBGY010000116 DAVBGY010000000
VERSION     DAVBGY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.116"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(697..1382)
                     /locus_tag="VQ620_002021"
                     /pseudo
     CDS             complement(697..1382)
                     /locus_tag="VQ620_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          383 a          282 c          286 g          431 t
ORIGIN      
        1 cagagtccag ttctatcaga ccgttcgttt tacagacaca agtgaagtag gattacttcg
       61 attagttgtt taccccttcg ctccacttcc attacagaag tttcctcact actatgggct
      121 cggctgactt ctcatgattc gttgttacta cgctttcggc gctcatgaga cctcacggga
      181 taagccgtac atctttcctc gtttatacaa gatatgagga cgttaaaagg ttacaatgaa
      241 aataggaaat ctgacgcaga gtctttagac tctaggaaga tttatctttt tcacatagac
      301 cttagtccga attcaattac tctcgtgatt tacgcatata ggttacgact acctttttgg
      361 actttagagt tttaagcccc cttatccgct gtatacgcct tactatcacg ttcctgttcg
      421 tagagcctcg atttcgctat cccttcctct gacgattctt atttcctgcg tcagacttaa
      481 aacggtctat ccccagaccg ttttaatccg ctacctcacg atagtcaggc ttgggaatcg
      541 ctattgggtt caccggtagc aggtgcccac ggaggactta cacctcagat gcacgacatg
      601 cccgtcgtac ttaaaatcag cctaaatgat tcaggctgat ttttctattg attcaaaaaa
      661 acggctcttt gtcaactgta gtgggttgaa gaaaagctaa gatcgagaaa ggacaaattt
      721 cgtcctttct tttttgatat tcagagcgat aaaaatccgt tttttgaagt tttcaaagtt
      781 ccgaaaacca aaggcattgt gcttgataag tttgatgaga ttattggtcg cttccaattt
      841 ggcgtttgaa tagggtagtt gaagggcgtt gataatcttt tctttatctt tgaggaaggt
      901 tttaaagaca gtctgaaaaa taggatgaac ctgcttcaga ttgtcctcaa taagtccgaa
      961 aaatttctct ggttccttat tctggaagtg aaaaagcaag agctgataga gctgatagtg
     1021 gtgtttcaag tcttccgaat agctcaaaag cttgtctaaa atctctttat tggttaaatg
     1081 catacgaaaa gtaggacgat aaaaacgctt atcactcagt ttacggctat cctgttgtat
     1141 gagcttccag tagcgcttga tagccttgta ttcatgggat tttcgatgaa actgattcat
     1201 gatttgaaca cgcacacgac tcatagcacg gctaagatgt tgcacaatgt gaaagcgatc
     1261 aagaacgatt ttagcattcg ggagtgaaac agtctgggag actgtttcag cctgagccta
     1321 gaaatttgaa agcgaagctg tctagctaag tcatagtaag ggctaaacat atccatagta
     1381 at
//
LOCUS       DAVBGY010000117         1242 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVBGY010000117 DAVBGY010000000
VERSION     DAVBGY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.117"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(570..644)
                     /locus_tag="VQ620_002022"
                     /pseudo
     CDS             complement(570..644)
                     /locus_tag="VQ620_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            802..1020
                     /locus_tag="VQ620_002023"
     CDS             802..1020
                     /locus_tag="VQ620_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HET2764591.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
BASE COUNT          397 a          198 c          252 g          395 t
ORIGIN      
        1 ctgacgcagg aaataagaat cgtcagagga agggatagcg aaatcgtggc tctacgaaca
       61 ggaacatgat aataaggcct atatagcgga taaggtatct ggctggggcg accactatca
      121 attatagtaa aatgaaataa gagtaggacg aattgatcag ggcagccaaa tcgatttcta
      181 acaatgtttt agaagaagag atgtactatt ctagtttcaa tctactatag tagctcagaa
      241 gtcggtactt aaacgtgcta tatcaaaacc agtccttgaa aaacgtggac tggtttcgtg
      301 tttggattat taccttgaac gacatgcgtt aacagttagt tgaaccgccg tatgccgaac
      361 ggcacgtacg gtggtgtgag aggggctaga gattatcccc tactcgattt cgaaatctag
      421 tggaatgaat ctggaatagt ccatcgagct ttctaatact cttcgaaaat ctcttcaaac
      481 cacgtcaacg tcgccttgcc gtgcgtatgg ttactgactt cgtcagttct atccacaacc
      541 tcaaaacagt gttttgagct gactacgtca gttccatcta caacctcaaa acagtgtttt
      601 gagcaacctg aggctagttt cctagtttgc tctttggttt tcatttagta taacagattg
      661 ttagaagttg gtttaaattt cctaatcagt ttgttcacat ttaccttcga tatattatat
      721 cccatagtta aggttggtca tacagatgat tatagtcatg gagccgtaaa acttagtgtt
      781 tctttagttg acaaagatgc catgaaaaaa atatctgtaa ctgtaatagg atattttgaa
      841 ataaatatag atgaaaatat caccgatatt ctatacgtaa atggtactgc tattctttat
      901 cattatttac gttctattgt ttcaatagtt tcggcaattg atagcagtga agcaatgttg
      961 ctacctacca ttaatgtttt agagttacta gataaatctc aaccttttga agaagaataa
     1021 tttattagct cactaaattg agggtaagga aaagtaaaat cagtaagaaa aatgtcttgc
     1081 attatacagc aaccttttgg gaatgagtag atggattgaa taaaatttta ttaagagtgg
     1141 atgatttatc tgtagattat tattggacag ttagtcttga agtagtctaa gaattaggtt
     1201 ataatcagta gaatccttgc taataatgag gaggttagtt ta
//
LOCUS       DAVBGY010000118         1159 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVBGY010000118 DAVBGY010000000
VERSION     DAVBGY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.118"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            436..1146
                     /gene="deoD"
                     /locus_tag="VQ620_002024"
     CDS             436..1146
                     /gene="deoD"
                     /locus_tag="VQ620_002024"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET2764592.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
BASE COUNT          340 a          212 c          259 g          348 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtataaac agaaaggtag agcgcgtgtt ctaatttgaa
       61 cacgagtaga aaacttttct aaaaacaaaa acgaaaggat gggtaaactg tattcgctga
      121 actgaatacg ggcgactctc ctctaaatca aaattaagaa agaaaggaat tgaccccacc
      181 ctaaaagcag tgggaaaaag atagttggtc tagcgagcat cgctcactgc gcccaactcc
      241 tattttccct tcgctttttg acgggtttgg tatctttctc aatataaaat ataaaataaa
      301 gaaaggtaga gcgtgtgttt tgatttgaac acgagcggaa aactcggaaa atagataatc
      361 tgactgaaaa atcaggattt ctcgtcggct tcctaatttt cagtcgtttt cttctcgctc
      421 tttgtatcat aaattatgtc tatccatatt gctgctcagc agggtgaaat tgctgataaa
      481 attcttcttc ctggggatcc tcttcgtgct aagtttattg cggagaattt ccttggtgat
      541 gctgtttgtt ttaacgaagt gcgtaacatg tttggttaca ctggtactta caagggtcac
      601 cgtgtatctg tcatgggaac tgggatggga atgccatcta tttcgattta tgcgcgtgag
      661 ttaatcgtag actacggtgt gaagaaattg attcgtgtgg gaactgcagg ttctttgaat
      721 gaagaggttc atgttcgtga attagttttg gcgcaggcgg ctgcaaccaa ctcaaacatc
      781 gttcgtaatg actggccaca gtacgatttt ccacaaattg ctagctttga tttgcttgat
      841 aaagcctacc atatcgccaa agaacttggt atgactactc acgttgggaa cgttttgtca
      901 tctgatgtct tttactcaaa ttactttgaa aagaatatcg agcttggtaa atggggagtc
      961 aaggctgtgg aaatggaagc agcagctctt tactatcttg ctgcccaata ccatgttgat
     1021 gcgctagcta tcatgaccat ctctgatagc ttggtcaatc cagacgaaga cacaactgca
     1081 gaagaacgtc aaaatacctt cactgatatg atgaaggttg gtttggaaac cttgattgca
     1141 gaataattat agccaaaaa
//
LOCUS       DAVBGY010000119         1138 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVBGY010000119 DAVBGY010000000
VERSION     DAVBGY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.119"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            26..>1138
                     /locus_tag="VQ620_002025"
     CDS             join(26..365,365..>1138)
                     /locus_tag="VQ620_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2764593.1"
                     /translation="MVKLIDRYGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSIS
                     LDYALPNQGMLSFWPAQYKKNGYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENE
                     RLRTEVAYLKKFKRVRGKGRSPRARKAETVREMVSGGFRLDFLLETARLARSTYYYQL
                     KQLDGVDKDKEIKTEIQGIDNEHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLT
                     ARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPV
                     LDGFNSEIIAYHLSTSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLE
                     SKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEK"
BASE COUNT          397 a          195 c          261 g          285 t
ORIGIN      
        1 tgtggatgtt tctggtctaa agtacatggt gaaattaatt gaccgttatg gcatagaaat
       61 cgttaaaaaa gggaagaatc gtcactattc ttctaaattg aaacaagaaa tgatggataa
      121 agccttactg gaaggttgtt cacaaagaag tattagtctt gattatgccc tcccaaacca
      181 aggaatgctt tcattttggc cggcacaata caagaaaaac gggtatacta ttgttgagaa
      241 aacaagaggg agaccagcta aaatgggacg taaacggaag aagacttggg aagaaatgac
      301 agaactagaa cgactccagg aggagaatga acgcttacgg actgaggtgg cctacctaaa
      361 aaagttaaaa gagttagagg aaagggacga agccctagag cgagaaaggc agagacagtt
      421 agagaaatgg tttcaggagg atttcgacta gattttcttc ttgaaacagc acgtttagct
      481 cgctcgactt actattatca gttgaaacaa ctggatgggg ttgataaaga taaagagatt
      541 aaaaccgaaa ttcagggcat tgataatgaa cataaaggca actatggcta tcgtcgaatc
      601 catttagaac taaggaatcg tggttttgtg gtcaatcata agaaagttca acggttgatg
      661 agaattcttg gtttaacggc tcgaattcgc cgtaaacgga agtattcttc ctaccaagga
      721 gagattggca agaaagcaga gaatctcatt caacgccagt tcgaagcatc gaggccaatg
      781 gaaaagtgct atacagatgt gacagagttt gccattccaa atagcacaca gaaactctat
      841 ttatcaccag ttttagatgg ttttaacagt gaaatcattg cttatcatct ttctacttcg
      901 ccaaacttag aacaagtgaa gagtatgtta gaacaggcct ttacagagaa atactatgag
      961 aatacgattc tccatagtga ccaaggctgg caataccaac acgattctta tcatcggttc
     1021 ctagagagta agggaattca agcatccatg tcacgcaagg gcaacagccc agacaacggc
     1081 atgatggaat ctttctttgg cattttaaaa tccgaaatgt tttatggcta tgagaaga
//
LOCUS       DAVBGY010000120         1109 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVBGY010000120 DAVBGY010000000
VERSION     DAVBGY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.120"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(<1..1057)
                     /gene="ltrA"
                     /locus_tag="VQ620_002026"
     CDS             complement(<1..1057)
                     /gene="ltrA"
                     /locus_tag="VQ620_002026"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET2764594.1"
                     /translation="MSKLLDKILSRDNMLEAYNQVKSNKGSAGIDGITIEEMDGYLRQ
                     NWRLTKELIKQRKYKPQPVLRVEIPKPNGGIRQLGIPTVMDRMIQQAIVQVISPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAAAKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVWRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSLGNMK
                     SIVASIDERLRTRLRVII"
BASE COUNT          289 a          243 c          199 g          378 t
ORIGIN      
        1 agataatcac tcgtaggcga gtacgcaagc gctcatctat gctggcgact atacttttca
       61 tatttcccaa tgagaaatag tttatccatc ctcgaataga caaattcagt tgctcaatac
      121 gtcttgttag gtctatactc catttcctct gtgttagttt cttcaattta agcttaaatc
      181 tccaaacact atcttgatgt ggacgacttt tccaaccatc tgatgatttc cagaacccga
      241 aacctagata tttcaactct cttggtctgg taatcttagt cttggtcatg tttactttca
      301 gacctagccg tttctcaata aaacgactga ctgaatacat cacacgctta gcggctgcct
      361 cgcttccgac cgtaatcaca caatcgtctg cgtagcgaac aaatcgaagc cctctctttt
      421 ctaattcctt gtccaattca ttaagcatga tattggataa gagaggagat aaatttcctc
      481 cctgtggcgt gccaactagc gttttataac gttgaccgtt aatgataaca cctgaatgaa
      541 gatatttacg aatcaaggat tccgtatctc cgtcttcgat aatgttatgt actaaggaca
      601 tcaatctatc ttgaggaact gtatcgaaaa atttctctag gtctatgtcc actatccact
      661 catagccgtc atttaagtac tctaagagct tcatgatggc tttttcacat gacctatttg
      721 gtctgaatcc atagctcgta tctgagaaat ggggttcaca aatagggcta ataacttgaa
      781 caatggcctg ttgaatcatt ctatccataa ctgttggaat tcctagttgg cggatgcctc
      841 cgtttggttt gggaatctca actctaagaa ctggttgagg cttatatttt ctctgtttta
      901 tcagttcctt agtcaggcgc cagttttgtc tgagatagcc atccatctct tcgatagtaa
      961 ttccatcaat cccagctgag cctttattag attttacttg attgtaggct tcaagcatat
     1021 tgtcgcgtga taatatttta tctagcaatt ttgacatgtg cctattcctt tcttgttttg
     1081 gtgcctgagg gacatcttat attggtgaa
//
LOCUS       DAVBGY010000121         1042 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVBGY010000121 DAVBGY010000000
VERSION     DAVBGY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.121"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            91..>1042
                     /locus_tag="VQ620_002027"
     CDS             91..>1042
                     /locus_tag="VQ620_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2764595.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
BASE COUNT          370 a          187 c          215 g          270 t
ORIGIN      
        1 ctgttgtcaa atgaaatgtg atttaaggta taagaaaaca cctctgtgct atacttgttg
       61 ttcaccacaa acacaaggaa aggcacagag atgcaagaac attatacccc aaaagggaaa
      121 catttgacaa tagataaccg tcgcttgatt gagcggtgga agaatgaaaa taagtccaat
      181 cgtgaaattg caggcttgtt aggaaaggcg cctcaaacga ttcataatga agtcaaaaga
      241 ggtacaactt tacaacaagt gagaaaaggg ctatacaaaa aggtctattc tgccgattac
      301 gcacaaactg tttaccaatt caatcgaaaa cggtcggtga aaaagttaat tttaacaaag
      361 gaaatcagag agaagatctt acactatcat aagcaaaaat tttcgcctga aatgatggtt
      421 aacaagaagc aagtgaaagt tggtatttca accatctact actggtttca taatggtcat
      481 ttaggattga cgaaggccga catgctttat cccagaaaaa ggaaaggtgt caagaagcaa
      541 gctagtccga actttaagcc ggcaggtaaa tctatcgaag aacgtcctga cgttattaat
      601 cttcgcttgg aaaatggtca ttatgaaatt gataccgtcc tactgactaa gataaaaaat
      661 tattgcctgt tagtcttaac cgaccggcgg agcagacacc aaattataag gttaattcca
      721 aataaaactg ctgaatctgt caatcaggcg cttacgttac tattagggga gcatcgtatt
      781 ctgtccatta ctgcagataa tggttcggag ttcaaacgat tgtctgaggt atttcctgag
      841 gaacatatct actacgcaca tgcttactct tcatgggaga gaggttcaaa tgaaaatcat
      901 aatcgattaa ttcggagatg gttacctaaa ggaaccaaga aaacgactcc gaaagaagta
      961 gcttttatcg aaaattggat taacaactac cctaaaaaat gcttggacta caagtcgcca
     1021 agtgaatttc ttttgggtgg ct
//
LOCUS       DAVBGY010000122         1014 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVBGY010000122 DAVBGY010000000
VERSION     DAVBGY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.122"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(419..817)
                     /locus_tag="VQ620_002028"
     CDS             complement(419..817)
                     /locus_tag="VQ620_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2764596.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLQIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT          322 a          197 c          145 g          350 t
ORIGIN      
        1 taacacagag gtgttttctt atgcctacaa gagttttcat tatttccatt ttctttcgga
       61 tttcactcta ttctgaaaaa cttgtgtata tttactgaag ctagcaagtc ttacctgtaa
      121 atttaatgaa agcaacacaa aatcctagag gggaatctcg gattaatgtc aatttaaaaa
      181 gatagagagt ttttagttta aataaattgt tttaaaatat caacaacatc acttcttttc
      241 ttaacctgat aagccttgat tcctaatttt ggagctacga ttatattgtc ctcaatatcg
      301 tctagaaaga cacaatttct aggttataac tggtatttat cgatagttac tcatatacat
      361 cagtccacct ccatacttat gtgcgagcct ctctttgtat tatacctcca tactcacctt
      421 acagattctt ttggtaataa tatctttgcc taatgtagag atagtcttgc aaagaaaaaa
      481 cttccttgta gccatgtttc tgataaaagt ccggtgcctg gaactgataa gtattgacaa
      541 aggcaaaaca acaatttcga ttcttagctt cactttctgc ctgttgcaat agttttgaac
      601 cgattccttg ccctcgcagt tcctctttta caaacaaata ctcgatttgt agccaatttc
      661 caaaagtctc tgctatcaaa cccgcaagga gattgccctt ttcatcttcg acataaagat
      721 taagtggctc actttcagcc tcttcccttt ttgaacggtt ataaacacga atcagattcc
      781 ctatttcttg cgatttatgt gattccttat tttccaatct aaagtataat gaaatgaaat
      841 aaaacatgcg caaatcgatt aaggaattta atctaatttc taataatgtc ttagaaatca
      901 aagtttacta ttttaacttc aatgcactat acatctaata ctcaatgaaa atcaaagagc
      961 aaactaggaa actagccgca ggttgctcaa aacactgttt tgaggttgta gata
//
LOCUS       DAVBGY010000123         1011 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVBGY010000123 DAVBGY010000000
VERSION     DAVBGY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.123"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(4..554)
                     /locus_tag="VQ620_002029"
                     /pseudo
     CDS             complement(4..554)
                     /locus_tag="VQ620_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(630..>1011)
                     /locus_tag="VQ620_002030"
     CDS             complement(630..>1011)
                     /locus_tag="VQ620_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2764597.1"
                     /translation="AHTIHQKFNLKVPNYRQEEDWARMGLPITRKEIANWHIKASQYY
                     LESLYNLLREKLLEQPLLHADETSYRVLESDSQLTYYWTFLSGKSEKQGITLYHHDQC
                     RSGSVVQEFLGDYSGYVHCDMLRQ"
BASE COUNT          288 a          237 c          182 g          304 t
ORIGIN      
        1 ctataattga tgacgtttag ctgtttccaa caaactcata ataatagctc ttgctttagc
       61 tccttcaaaa ctttgagaaa ataaccaccc tcgctttttt aaactgagct taggctaaaa
      121 gagtccactg gactctttta ctccgtccca taaccaatga tttaatggcg cattcagcta
      181 gattattgga aaggaccaga tgtccgtctt tcaaaatagt cttaaaggtt tctttatact
      241 tgaggctgta ttcaattgcc cttcctagtt ttgaacccga taaaactgac tgacgccggc
      301 accaagcaaa gaaatcttcc attaggttgg agctcttctt gacgtttctg tagtcgttca
      361 tcagctggca aagcctccca gtctctttcc aagaaaaata actgatcaca ataagctaaa
      421 cctttagctc ctaaggatga tttatccgct tgcttggggg tcgcttcaaa aaacttcctt
      481 ctcacatgcg cccaacatcc aacaagtgca gcttcttcca gttgacgata agcttctagc
      541 ggttcgcagt ttaccaagct tagcgtcgcc ttgctcctaa accttggact gctatcgcat
      601 aggcagaact agaggactaa agtcctaagt tactgccgca acatatcaca atgcacatag
      661 ccagaataat ctcctaggaa ttcttgtact actgaaccac ttcgacactg atcatggtgg
      721 taaagcgtaa tcccttgttt ctctgattta cctgacaaaa aagtccaata gtaagtcagc
      781 tgactatcac tttctaagac ccgataagag gtttcatctg catgaagaag aggttgttct
      841 aacaactttt ctcgtaaaag gttataaagg gactctaaat agtattgact cgccttgata
      901 tgccaattag caatttcctt acgtgtgatt ggtaaaccca tcctagccca atcttcttct
      961 tggcgataat taggtacctt cagattaaac ttctgatgga tggtgtgagc g
//
LOCUS       DAVBGY010000124          923 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVBGY010000124 DAVBGY010000000
VERSION     DAVBGY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.124"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            123..591
                     /locus_tag="VQ620_002031"
                     /pseudo
     CDS             123..591
                     /locus_tag="VQ620_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          336 a          139 c          173 g          275 t
ORIGIN      
        1 ggagaagagg attgccatct tctccatttt tatgtgcaga gttatagtgg cttgatgctg
       61 ggaaagtaca ctgtgactgc taaaacattc ctagaagctg ctttgatttc cctaatctat
      121 ttatgcaaat tttatgttat tttactataa cagttgttgc taagccaaat aatagtgggg
      181 aagttcattt agacgtaagc attgaagata atcagggagg tagtgggtat aatttcagtt
      241 ctgtttcaag tagctcacaa acagctaaat atgaaggaac tggttataat aacaattcat
      301 cattatatat aacgattgat aaaacgtctg atgcaacagc tcttttgaaa ttaaagttga
      361 ataatgttga taatcaacct gctactgaag ttcctagttc aggaattact gtaaaattaa
      421 atgctaaaga taatgctgga aactggacaa gtgcttcgaa taaagacaag tgcttcgaat
      481 aaaaaagaaa taacagtaaa aattgtttct gctaaaccga catatccaga caaaatctta
      541 gtgaaaaatc ctgataatat aaaagataca gaaaaaaatg ccattattga aaaattgaaa
      601 gaggcaaata aaaatcatcc agcaggagct ccaacctttg ctaaaggtga aggagagcat
      661 gcaaatgata ttgtagcaac ttattcagat ggtacaactt attatgtacc gttaaatgat
      721 gtgacaaaat atgcgaggta gtggctgtac cactcactta ttcacctccc cgtgatttgt
      781 agtagtgata ggctttctca ctattattat aaaacaaaat aaagatcaca acactttttc
      841 attctgtgtt gtgccttgag tgaaacgaaa ggaatgaatt ataaatatga aaagtatagt
      901 cactagcata gatgagcgct tgc
//
LOCUS       DAVBGY010000125          844 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVBGY010000125 DAVBGY010000000
VERSION     DAVBGY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.125"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            123..656
                     /gene="queF"
                     /locus_tag="VQ620_002032"
     CDS             123..656
                     /gene="queF"
                     /locus_tag="VQ620_002032"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HET2764598.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWTMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
BASE COUNT          258 a          194 c          172 g          220 t
ORIGIN      
        1 aagtagaata ttacgatgtt tgggatatgc catcgctaaa aaaacctcga tttggtattt
       61 tttagctccc taatgggagc tttttgcgtg ctctgaacat ttcccatttt ggaaggagta
      121 ctatgaaacg tcaatcagcc ttggtcgtct ttagtggcgg tcaagattca acaacctgcc
      181 ttttctggac catgcaacac tatgaaacag tcgaagctgt cacctttgcc tacggccaac
      241 gtcatcacct cgaaattcaa attactagag aaatcgctaa ggaacagggc attcgtcacc
      301 atatcctcga tatgtctctg ctgggacaaa tcactgctca gccagacttt gcgacgattc
      361 atatttccta cattcctgac aagctctgtg tcgagtcaaa atccctcaaa ctatatctat
      421 ttagctaccg aaatcacgga gatttccacg aaaactgtat caacaccatc gggaaagact
      481 tggtcaactt gctagaccct cgctatttag aagtctgggg aaaattcact ccgcgcggtg
      541 gcatttcaat cgacccctac tataactacg gtaagcaagg aactaagtat gagggcttgg
      601 cagaacaacg cctcttccaa cacgaccttt atccagagaa aattgacaac cgctaaagtc
      661 atactcaatg aaaatcaaag agcaaactag gaagttagcc gcaggttact caaagcagtg
      721 tttgaggttg tagataaaac tgacgaagtc agctcaaaac actgttttga ggttgcagat
      781 agaactgacg aagtcagctc aaaacactgt tttgaggttg tggataaaac tgacgaagtc
      841 agct
//
LOCUS       DAVBGY010000126          830 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVBGY010000126 DAVBGY010000000
VERSION     DAVBGY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.126"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            21..830
                     /locus_tag="VQ620_002033"
                     /pseudo
     CDS             21..830
                     /locus_tag="VQ620_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
BASE COUNT          266 a          174 c          162 g          228 t
ORIGIN      
        1 aaaagtgtta tactattttt atggaaacag tatacgacaa agcacaaaaa cttaactcaa
       61 aaaacttcaa actattgatt ggtgtcaaaa aggaaacctt tcaactcatg ctagaacacc
      121 tgaattcagc ctatcagatt cagcaccgaa aaggtggacg tccacgtagt ctgcccatgg
      181 aagaccagct cattatgacc ctccgttact tgcgatatta tcccactcag cgtctgctgg
      241 cctttgattt tggcgtcggt gtagctacgg taaatgccat catcacttgg gtggaggata
      301 cacttcgtgc gtcaggtagc tttgatttgg accatttaga agccccgagt gctgctgtgg
      361 ctattgacgt gaccgaaagt ccgattcagc gtccaaaaaa aaccaaagca aaaattattc
      421 tggtaaaaag aaacgacaca ccttaaaaac tcaaattatg ctggatttga cgacacataa
      481 agtctgtcaa atggcctttt ctgacggaca tacgcatgat tttactctct tcaaagaaag
      541 tattggacaa agtttgcctg aaacgacgct tgcctttgtt gacctaggtt atttaggcat
      601 cttgaaattt catgagaata ctttcattcc tgctaaaaat tccaaaaatc gccgcctgag
      661 tgaggatgat aagcagttaa ataaagagat gtcagcgata cgaattgaaa ttgaacattt
      721 taacgctaaa ttcaagacct tccaaatcat gtcagtccct tatcgtaacc gcagaaaacg
      781 tttcgagtta cgggcggaat taatttgtgc catcatcaat tatgaagtga
//
LOCUS       DAVBGY010000127          768 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVBGY010000127 DAVBGY010000000
VERSION     DAVBGY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.127"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(74..147)
                     /locus_tag="VQ620_002034"
     tRNA            complement(74..147)
                     /locus_tag="VQ620_002034"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Arg,seq:acg)
     gene            complement(157..242)
                     /locus_tag="VQ620_002035"
     tRNA            complement(157..242)
                     /locus_tag="VQ620_002035"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(206..208),aa:Leu,seq:taa)
     gene            complement(250..321)
                     /locus_tag="VQ620_002036"
     tRNA            complement(250..321)
                     /locus_tag="VQ620_002036"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Gly,seq:gcc)
     gene            complement(344..416)
                     /locus_tag="VQ620_002037"
     tRNA            complement(344..416)
                     /locus_tag="VQ620_002037"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(381..383),aa:Thr,seq:tgt)
     gene            complement(435..516)
                     /locus_tag="VQ620_002038"
     tRNA            complement(435..516)
                     /locus_tag="VQ620_002038"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(480..482),aa:Leu,seq:tag)
     gene            complement(522..594)
                     /locus_tag="VQ620_002039"
     tRNA            complement(522..594)
                     /locus_tag="VQ620_002039"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(559..561),aa:Lys,seq:ttt)
     gene            complement(619..691)
                     /locus_tag="VQ620_002040"
     tRNA            complement(619..691)
                     /locus_tag="VQ620_002040"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(656..658),aa:Asp,seq:gtc)
     gene            complement(694..766)
                     /locus_tag="VQ620_002041"
     tRNA            complement(694..766)
                     /locus_tag="VQ620_002041"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(731..733),aa:Val,seq:tac)
BASE COUNT          196 a          227 c          168 g          177 t
ORIGIN      
        1 cgaacctgcg acaccttggt cccaaaccaa gtactctacc aagctgagct acttcccgag
       61 ttaaatagaa aaatgcaccc tagaggagtc gaacctctaa ccgcctgatt cgtagtcagg
      121 tactctatcc agttgagcta agggtgctcc atattatgcc gaggaccgga atcgaaccgg
      181 tacgatcgtt accaatcgca ggattttaag tcctgtgcgt ctgccagttc cgccaccccg
      241 gcctctctaa gcgaacgacg ggattcgaac ccgcgacccc caccttggca aggtggtgtt
      301 ctaccactga actacgttcg cactgttttc ttctatctaa aaatgccggc tacatgactt
      361 gaacacgcga ccctctgatt acaaatcaga tgctctacca actgagctaa gccggctcat
      421 ttgttatatc ttaatgcggg ttaagggact tgaaccccca cgccgttaag cgccagatcc
      481 taaatctggt gcgtctgcca attccgccaa acccgcatat atgacccgta ctgggctcga
      541 accagtgacc cattgattaa aagtcaattg ctctaccaac tgagctaacg agtctaaaat
      601 aacttgcgtt accttaaacg gtcccgacgg gaatcgaacc cgcgatcttc gccgtgacag
      661 ggcgacgtga taaccgctac actacgggac ctttgggagt taacgggatc gaaccgctga
      721 ccctctgctt gtaaggcaga tgctctccca gctgagctaa actcccta
//
LOCUS       DAVBGY010000128          670 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVBGY010000128 DAVBGY010000000
VERSION     DAVBGY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.128"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            43..>670
                     /locus_tag="VQ620_002042"
     CDS             43..>670
                     /locus_tag="VQ620_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2764599.1"
                     /translation="MLYVGIDIAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALKLLSDPNREQFQHDNRQVDLKILARHIHRLKKKQSDWKVQYTRCLD
                     IIFPELDKIVGKHSEYTYQLLTRYPNPQKRIEAGFDKLIEIKRLTASKIQDILSVAPR
                     SIGTTSPAREFEIIEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIG"
BASE COUNT          214 a          147 c          121 g          188 t
ORIGIN      
        1 ataaccaagt agacttgaaa tactatatct ggaggtactc ccatgcttta tgtgggcatt
       61 gatatcgcta aaaataaaca cgatgttaca gccttgaatg ttccaggaaa aactgttctt
      121 aaaccactca ctttttcaaa taataaagct ggttttgaac tcttagatct gtctcttcga
      181 cagctcaacc aagactgtct catcgctctt aaacttcttt ctgaccccaa tcgtgaacaa
      241 tttcaacacg ataatcggca agtagaccta aaaatactgg ctagacatat tcatcgtctc
      301 aagaaaaaac agtctgattg gaaagtacaa tacactcgtt gtcttgatat catctttcct
      361 gagttggata aaatcgttgg aaagcattca gaatatacct accaactctt gacgcgctac
      421 cctaatcctc agaaaaggat tgaggcagga tttgataagc tgatagaaat taagcgattg
      481 accgcttcta aaattcagga tatcctctca gtcgcacctc gttctatcgg aacaacatct
      541 cctgctcgtg aattcgaaat catcgaaatc atcaaacatt acaagaggct cattgacaag
      601 gcggaaacat gtgtcaatga cttgatggct gagttcaact cagtcatcac gacggttact
      661 gggattgggg
//
LOCUS       DAVBGY010000129          645 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVBGY010000129 DAVBGY010000000
VERSION     DAVBGY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.129"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            216..398
                     /locus_tag="VQ620_002043"
     CDS             216..398
                     /locus_tag="VQ620_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET2764600.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            343..>645
                     /locus_tag="VQ620_002044"
     CDS             343..>645
                     /locus_tag="VQ620_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HET2764601.1"
                     /translation="MKKTLTPTKESIDSLRNYEASKQLTDARFKRLVGVQRTTFEEML
                     AVLKTAYQLKHTKGGRKPKLSLEDLLMATLQYVREYRTYEEIAADFGIHESNLIRRS"
BASE COUNT          256 a          110 c          109 g          170 t
ORIGIN      
        1 tatatcattt ttaaatttta agtcaagaaa aattaaccca ctttaatttc ttttttaacc
       61 cgtgttaaaa aagaaattaa agtgggttaa aaaagaaatt aaagtgggtt aaaaaagaaa
      121 ttaactgagt aaaaactctc gaaattcaat tcgctatatt atcactcaac ttgaacaaat
      181 accagaacca caaaaaacag atgtaaagga aaaatatgga cttaaaattt gaaggggttg
      241 atttagaata caaaaaagct aaaaacaatc ttccagagtc tttctgggaa acctactcag
      301 catttgcaaa tacaaatggc gggaaaataa ttctcgggat agatgaaaaa aacattgaca
      361 cctaccaaag agtcaataga ctccctgcga aattatgaag caagtaaaca actaaccgat
      421 gcacgattta agcgtcttgt tggtgttcag cgcaccactt ttgaagagat gttagctgtg
      481 ttaaaaacag cttatcaact taaacacaca aaaggtggac gaaaacctaa attaagccta
      541 gaagaccttc ttatggccac tcttcaatat gtgcgagaat accgcactta tgaagaaatt
      601 gcggctgatt ttggtattca cgaaagcaac ttaatccgtc ggagc
//
LOCUS       DAVBGY010000130          624 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVBGY010000130 DAVBGY010000000
VERSION     DAVBGY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.130"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            118..312
                     /locus_tag="VQ620_002045"
     CDS             118..312
                     /locus_tag="VQ620_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2764602.1"
                     /translation="MKIIQQQSATIDSLTNALALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLNLFEEESPSEKDGDVPS"
     gene            299..604
                     /locus_tag="VQ620_002046"
     CDS             299..604
                     /locus_tag="VQ620_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HET2764603.1"
                     /translation="MFPVETEEITYKRKKSKGKRQALLAQFDSEEVHHQVEESICSDC
                     QGDLKEIGATLQRQELVFIPAQLKRIDHIQHAYKCQACSDKNPSDKIVKAPIPKAPL"
BASE COUNT          218 a          126 c          117 g          163 t
ORIGIN      
        1 cttttctatc actccaaaaa taaatccatc agaaagtcgt gatttctatt gaaatgagga
       61 ctttcttttt agtataataa aggtagaaaa caaggagggg aagcccatgg aagaagattg
      121 aaaatcattc aacaacagag tgctacaatt gatagtctca ccaatgcact tgcccttctt
      181 cgtgaacaag tggcttatct gactcaaaag ctctatggaa aatcctctga gaaaagtgtt
      241 tgcccctctg gacaactcaa cctttttgag gaagagtctc catctgagaa agatggagat
      301 gttcccagtt gaaacagagg aaattaccta caaacgtaag aaatctaaag ggaaacgcca
      361 agctcttctt gcccaatttg attcagaaga agttcatcat caagtagaag agagcatttg
      421 ctctgattgt cagggagatc taaaagagat tggagcaacc cttcaacgac aagaattagt
      481 ctttattcct gcgcaattaa aacgcataga tcatatccaa cacgcttaca aatgccaagc
      541 atgcagtgat aaaaatccga gtgataaaat tgtgaaagct cctattccta aagccccttt
      601 ataacgagac tattgactca gctt
//
LOCUS       DAVBGY010000131          543 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVBGY010000131 DAVBGY010000000
VERSION     DAVBGY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.131"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..223
                     /locus_tag="VQ620_002047"
     CDS             <1..223
                     /locus_tag="VQ620_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2764604.1"
                     /translation="QAQTPRKSSKLKPLTAEDKACNHVLSKERSKVENIFAKVKTLKM
                     FSTTYRNHRKRFGLRMNLIVGIINHELGF"
     gene            240..543
                     /locus_tag="VQ620_002048"
                     /pseudo
     CDS             240..543
                     /locus_tag="VQ620_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          207 a           83 c           97 g          156 t
ORIGIN      
        1 tcaagcacaa actccacgta aatccagcaa actcaagccg ctaacagctg aagataaagc
       61 ctgtaaccat gtgctatcta aggagagaag caaggttgag aacatctttg ccaaagtaaa
      121 aacgcttaaa atgttttcaa caacctatcg aaatcatcgt aaacgcttcg gattacggat
      181 gaatttgatt gttggtatta tcaatcatga actaggattc tagttttgca ggaagtctat
      241 tatcaaaaat accatcagga ttatataaga ttgatacagg aaaagtttta ttttatggta
      301 taaatattaa tcaaatagat aaaaaaatat taagtcaaaa tttaggagta gttccacagg
      361 attcattttt attgaaccga agtattcttg ataatataac tttaaagcac gaagttactt
      421 cacaaaagat agaggaagtt tgtaaagcag ttcaaatcta tgatgaaatc atggctatgc
      481 cgatgaaatt taatactatc atctcagaga tggggtcaaa tatttcaggt gggcaaaggc
      541 aac
//
LOCUS       DAVBGY010000132          527 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVBGY010000132 DAVBGY010000000
VERSION     DAVBGY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.132"
                     /strain="2041"
                     /db_xref="taxon:1313"
BASE COUNT          169 a           87 c          138 g          133 t
ORIGIN      
        1 ctgactatcg tgaggtagca gggagaggaa gggatagcga aatcgtggct ctacgaacag
       61 gaacgtgata gtaaggcgta tatagcggat aaggaggctt caaactctaa agtccaaaaa
      121 ggtagtcgta acctatatgt gtaaatcacg agagtaattg aattcggact aaggtttgtg
      181 tgaaaaagat aaatctttct agagtctaaa gactctgcgt cagatttcct attttcactg
      241 taacctttta acgtcctcat atcttgtata aacgaggaaa gatgtacgac ttatcccgtg
      301 aggtttcatg agcgctgaaa gcgtagtaac aacgaatcat gagaagtcag ccgagcccat
      361 agtagtgagg aaacttccgt aatggaagtg gagcgaaggg gtgaatactc aaacagtctg
      421 gggagagact gtttgaggtc tgtcgctaga aagagaaaac gacagatcga agtaatccta
      481 cttcacttgt gtctgtaaaa tgagtggtct gatagaactg gactttg
//
LOCUS       DAVBGY010000133          526 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVBGY010000133 DAVBGY010000000
VERSION     DAVBGY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.133"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(238..325)
                     /locus_tag="VQ620_002049"
     tRNA            complement(238..325)
                     /locus_tag="VQ620_002049"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(289..291),aa:Ser,seq:gct)
     gene            complement(332..405)
                     /locus_tag="VQ620_002050"
     tRNA            complement(332..405)
                     /locus_tag="VQ620_002050"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(369..371),aa:Ile,seq:gat)
     gene            complement(441..511)
                     /locus_tag="VQ620_002051"
     tRNA            complement(441..511)
                     /locus_tag="VQ620_002051"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(477..479),aa:Gly,seq:tcc)
BASE COUNT          176 a          118 c           92 g          140 t
ORIGIN      
        1 gcaatgcctt tggttttcga aactttgaaa actttaaaaa acggattttt atcgctttga
       61 acatcaaaaa agaaaggacg aaatttgtcc tttctcgagc ttagcttttc ttcaacccac
      121 tacagttgac aaagagccat aaaaataact tcagattagt aattcaatta agttttacgg
      181 atctgaagct ttattgtctc taaaaataaa aaagagagtt acaaactctc attaaaatgg
      241 agaataaggg attcgaaccc ttgcgccagt tacccgacct aacgatttag caaaccgtcc
      301 tcttcagcct cttgagtaat tctccaatta atgggcacga gtggactcga accaccgacc
      361 tcacgcttat caggcgtgcg ctctaaccac ctgagctacg cgcccaagtt aaaaaacttg
      421 gtaatttgaa caaagttcaa agcgggtgac gagaatcgaa ctcgcgacaa cagcttggaa
      481 ggctgtagtt ttaccactaa actacacccg cataaatact atcaat
//
LOCUS       DAVBGY010000134          518 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVBGY010000134 DAVBGY010000000
VERSION     DAVBGY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.134"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..101
                     /locus_tag="VQ620_002052"
     CDS             <1..101
                     /locus_tag="VQ620_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2764605.1"
                     /translation="FKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            304..>518
                     /locus_tag="VQ620_002053"
     CDS             304..>518
                     /locus_tag="VQ620_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HET2764606.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAA"
BASE COUNT          187 a           67 c          118 g          146 t
ORIGIN      
        1 cgtttaaaat gttttcaaca acctatcgaa atcatcgtaa acgcttcgga ttacgaatga
       61 atttgattgc tggtattatc aatcatgaac taggattcta gttttgcagg aagtctaata
      121 gtaaaaaagt gattagaaaa catctttttt aaaaatagag atgattttga aacaaaaaag
      181 ctaattcaag acgtttcgat gccaattcaa gatttggatg aaaaaaatta atagatactg
      241 ttatactaaa cttgtcaagt ttgtaacaag acaaatatta aaaataaaaa agaggtattc
      301 gttatgaata caaaaaagat gtcacaattt gaaattatgg atactgagat gcttgcttgc
      361 gttgaaggtg gcggatgcaa ttggggagat tttgccaaag caggtgttgg aggaggagca
      421 gtacgaggtc ttcagctagg aattaaaaca agaacatggc aaggtgcagc aactggtgct
      481 gcgggaggag ctatacttgg aggtgtggcc tatgcagc
//
LOCUS       DAVBGY010000135          500 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVBGY010000135 DAVBGY010000000
VERSION     DAVBGY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.135"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(4..381)
                     /locus_tag="VQ620_002054"
     CDS             complement(4..381)
                     /locus_tag="VQ620_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET2764607.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIALV
                     YAIAIVDSNYFSDDLAFHSFIVK"
BASE COUNT          144 a           94 c           96 g          166 t
ORIGIN      
        1 atttcatttt actataaaac tatgaaaagc taggtcatct gagaagtaat tactatcgac
       61 aattgcgata gcgtatacta gtgcgatttg tttgtagctc tggtgggtat caccttcacg
      121 ttggcgaatt ttttctaggt tttgattaac ctgactgcac agataagccc ataggcgatt
      181 gatgaaaaaa ttctgatgat gaacctgaat ttcgatgata acttggattc cattatctaa
      241 ctccgccaag acgtctatac tagtatagaa atcttgcgct gagtagggca tggaaggcaa
      301 gacgtgaatg ttacttccct ccaaaatcgt cacatttttt gctggcaagt ccagcatatc
      361 gcggataaat tgacaaatca ttagttaggc tgatacctgg atgtctgaga atcctccttt
      421 tcctccttct attatagcac atttttaaat ctaggtttgc tagattctat gcttctatct
      481 atttattcgg aaaagaaagc
//
LOCUS       DAVBGY010000136          438 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVBGY010000136 DAVBGY010000000
VERSION     DAVBGY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.136"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..>438
                     /locus_tag="VQ620_002055"
     CDS             <1..>438
                     /locus_tag="VQ620_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2764608.1"
                     /translation="FGDVILNEKEWKVSKWNEILTIRNGKNQKQVEDADGKFPIYGSG
                     GIMGYAKDWIVKKNSVIIGRKGNINKPILVRENFWNVDTAFGLEPVLEKINSEYLFYF
                     CQLYNFEKLNKAVTIPSLTKSDLLNISIPLPPLALQNEFADFVA"
BASE COUNT          166 a           53 c           83 g          136 t
ORIGIN      
        1 gtttggggat gttattctaa atgaaaaaga atggaaagtt agcaaatgga acgagattct
       61 aacaataaga aatggtaaaa atcaaaaaca ggttgaagat gctgatggga aatttcctat
      121 ttatggaagt gggggaatta tgggatatgc taaagattgg atagtgaaga aaaattcagt
      181 tattattgga cgtaaaggaa atataaataa acctatttta gttagagaaa atttttggaa
      241 tgtagataca gcatttggat tagagcctgt tttagaaaaa ataaactcag aatatttatt
      301 ctatttttgt caattataca attttgagaa gttaaataag gctgtaacaa tacctagttt
      361 aactaaatca gatttattaa atatttccat ccccctcccc ccactagccc tccaaaatga
      421 gttcgcagac tttgtagc
//
LOCUS       DAVBGY010000137          416 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVBGY010000137 DAVBGY010000000
VERSION     DAVBGY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.137"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..>416
                     /locus_tag="VQ620_002056"
     CDS             <1..>416
                     /locus_tag="VQ620_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2764609.1"
                     /translation="STLVEIVRGGSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNV
                     KEKIKKSGLNKTRFVKKGTFLLTNSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDY
                     LFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILI"
BASE COUNT          149 a           50 c           72 g          145 t
ORIGIN      
        1 tttctacatt ggttgaaatt gtcagaggtg gctctccacg accaatcaaa gattatctta
       61 cttctgaagt agatggaata aattggataa aaataggtga tactgaaaag ggtgaaaagt
      121 atataaataa tgttaaagaa aaaatcaaaa aatcagggct taacaaaact agatttgtaa
      181 aaaaaggtac atttttgtta actaattcta tgagttttgg tagaccttat attttgaatg
      241 ttgatggtgc aatacacgat ggatggttgg ctatttcgaa ctatgaaaac tcattaaata
      301 aagattacct attctatatt ctttcatcaa atgtagttta ttctcaattt ctatctctaa
      361 ttagtggagc tgttgtgaaa aacttgaata gtgataaagt tgcttctatt cttatc
//
LOCUS       DAVBGY010000138          406 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVBGY010000138 DAVBGY010000000
VERSION     DAVBGY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.138"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..345
                     /locus_tag="VQ620_002057"
     CDS             <1..345
                     /locus_tag="VQ620_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2764610.1"
                     /translation="LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRL
                     EKPVTFHNMIAPDKEVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEP
                     EKLYAYIAEAIA"
BASE COUNT          138 a           71 c           71 g          126 t
ORIGIN      
        1 ttgatcacgc gtgaaaagtc atttccaact ggtttagata tggaatttct aggaaaggac
       61 ttgccaaatg tagcgattcc tcatacagat attgttcata atctagctga gaaagtggtg
      121 gttgttcgat tagaaaaacc agtaactttt cacaatatga tagctcctga taaggaagta
      181 gaagtatcct tgctcttctt tatcatcaat aactcaagtt caagtcaaac aaatattctg
      241 gctcagttga tggacttttt cacaggaaat ggacatcttg aagacctatc aaaaatttcc
      301 gaaccagaaa aactttatgc ttacattgct gaagcaatcg cttaatcttg tctattaaaa
      361 aataaaatcg gaggaaatct aaatgattaa aattcttgct gcctgc
//
LOCUS       DAVBGY010000139          398 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVBGY010000139 DAVBGY010000000
VERSION     DAVBGY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.139"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..>398
                     /locus_tag="VQ620_002058"
     CDS             <1..>398
                     /locus_tag="VQ620_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HET2764611.1"
                     /translation="KSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQA
                     PSRARKLVSQNSVLFSTVRPYLKNIAVVRELKEYLIASTAFIVLDTLLNETYLKYYLL
                     SDNFINRVNNKSTGTSYPAINDYNFNLLLI"
BASE COUNT          151 a           53 c           53 g          141 t
ORIGIN      
        1 taaaatcaat ttattggaat tttgggcaaa ataagccaga gaaatccttt aggtatatag
       61 atacgtctag tattgataga aaaaagaata taatcaacta caaaaatcta caatatcttt
      121 cacctgaaca agcgccttcc cgtgctagaa aattagtttc gcagaatagt gtcttatttt
      181 caacagttag accatatcta aaaaatattg ctgtagttag agaacttaaa gagtatttga
      241 tagctagtac agcatttatt gttttggata ctttacttaa cgaaacatat ttgaaatatt
      301 atttattatc agataatttt atcaatcgtg ttaataataa atctactgga acaagttatc
      361 ctgcaatcaa tgattataat tttaatctat tattaatt
//
LOCUS       DAVBGY010000140          393 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVBGY010000140 DAVBGY010000000
VERSION     DAVBGY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.140"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..>393
                     /locus_tag="VQ620_002059"
     CDS             <1..>393
                     /locus_tag="VQ620_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET2764612.1"
                     /translation="YLHLWELSDWDQEGMLCLHELISREEGLVDDIPRLRKYFKTKFR
                     NRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLWLDDYYLFHETLRDYRNKQSK
                     EKQEELERVLSNERFRGRQRVLRDLRIVF"
BASE COUNT          143 a           57 c           93 g          100 t
ORIGIN      
        1 taccttcatt tatgggaatt gtcggattgg gaccaagaag gcatgctctg cttacatgaa
       61 ttgattagta gagaagaagg actggtagac gatattccac gtttaaggaa atatttcaaa
      121 accaagtttc gaaatcgaat tttagactat atccgtaagc aggaaagtca gaagcgtaga
      181 tacgataaag aaccctatga agaagtggga gagatcagtc atcgtataag tgaggggggt
      241 ctctggctag atgattatta tctctttcat gaaacactaa gagattatag aaacaaacaa
      301 agtaaagaga aacaagaaga actagaacgc gtcttaagca atgaacgatt tcgagggcgt
      361 caaagagtat taagagactt acgcattgtg ttt
//
LOCUS       DAVBGY010000141          310 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVBGY010000141 DAVBGY010000000
VERSION     DAVBGY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.141"
                     /strain="2041"
                     /db_xref="taxon:1313"
BASE COUNT           87 a           38 c           74 g          111 t
ORIGIN      
        1 cgttgcggta actggagtgc ttttagttgg gaaaattgtt aattcattta aattgtaaac
       61 tttagggatt ctctgaagtt atggatgtcg ttccaatatg tgtacgttgg aatgttagtg
      121 cttatatggg gggaacagaa tcctctcttg attgaagaca agttagccat taggctggtt
      181 tgtctctttg tcaactgtag tgggttgata taatagtatt agtgagtggg ataaaagttt
      241 catttagttt attcagtaca aatttaacgg gtcaagattt attgaactgc accccaatag
      301 ttagacagaa
//
LOCUS       DAVBGY010000142          306 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVBGY010000142 DAVBGY010000000
VERSION     DAVBGY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.142"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            complement(<1..>306)
                     /locus_tag="VQ620_002060"
     CDS             complement(<1..>306)
                     /locus_tag="VQ620_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2764613.1"
                     /translation="LIQQDSRKLSDKRFYRPTFRMHLTNKEILDKLLSYSQDLKHHYQ
                     LYQLLLFHFQNKEPEKFFGLIEDNLKQVHPLFQTVFKTFLKDKEKIVNALQLPYSNAK
                     "
BASE COUNT           91 a           46 c           69 g          100 t
ORIGIN      
        1 tttggcgttt gaatagggta gttgaagggc gttgacgatt ttctctttgt cctttagaaa
       61 ggttttaaag acagtctgaa aaagaggatg aacctgcttt agattgtcct caatgagtcc
      121 gaaaaatttc tccggttcct tattctgaaa gtgaaacagc aagagttgat agagctgata
      181 gtgatgtttc aagtcttgtg aatagctcaa aagcttgtct aaaatctctt tattggttaa
      241 atgcatacga aaagtaggac gataaaatcg cttatcactc agtttacggc tatcctgttg
      301 aatgag
//
LOCUS       DAVBGY010000143          288 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain 2041
            SAMEA1677958-rid20186723.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVBGY010000143 DAVBGY010000000
VERSION     DAVBGY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1677958
            Sequence Read Archive: ERR226332
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980147.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980147.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 02:26:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,025
            Genes (coding)                    :: 1,904
            CDSs (with protein)               :: 1,904
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 56 of 121
            Pseudo Genes (incomplete)         :: 59 of 121
            Pseudo Genes (internal stop)      :: 30 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1677958-rid20186723.denovo.143"
                     /strain="2041"
                     /db_xref="taxon:1313"
     gene            <1..>288
                     /locus_tag="VQ620_002061"
     CDS             <1..>288
                     /locus_tag="VQ620_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2764614.1"
                     /translation="NRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEW
                     FDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGFGNASDHVQRNKNG"
BASE COUNT           93 a           59 c           59 g           77 t
ORIGIN      
        1 caaccgcgtg aaagcagcta agaaggtgcc acttgatcgt atgccttaca atcttcaata
       61 tactgtagaa gtcaaaaacg gtagtttaat catacctcat tatgaccatt accataacat
      121 caaatttgag tggtttgacg aaggccttta tgaggcacct aaggggtata ctcttgagga
      181 tcttttggcg actgtcaagt actatgtcga acatccaaac gaacgtccgc attcagataa
      241 tggttttggt aacgctagcg accatgttca aagaaacaaa aatggtca
//
